LOCUS       DACECY010000001        92366 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.001, whole genome shotgun sequence.
ACCESSION   DACECY010000001 DACECY010000000
VERSION     DACECY010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 92366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 92366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..92366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.001"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(203..1654)
                     /gene="cls"
                     /locus_tag="IWO38_000001"
     CDS             complement(203..1654)
                     /gene="cls"
                     /locus_tag="IWO38_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ0312124.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1995..4661
                     /locus_tag="IWO38_000002"
     CDS             1995..4661
                     /locus_tag="IWO38_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0312125.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5025..5777
                     /locus_tag="IWO38_000003"
     CDS             5025..5777
                     /locus_tag="IWO38_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0312126.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5799..6953)
                     /locus_tag="IWO38_000004"
     CDS             complement(5799..6953)
                     /locus_tag="IWO38_000004"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ0312127.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7138..9579
                     /locus_tag="IWO38_000005"
     CDS             7138..9579
                     /locus_tag="IWO38_000005"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ0312128.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9909..10613)
                     /locus_tag="IWO38_000006"
     CDS             complement(9909..10613)
                     /locus_tag="IWO38_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312129.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10800..11045)
                     /locus_tag="IWO38_000007"
     CDS             complement(10800..11045)
                     /locus_tag="IWO38_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312130.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(11062..11913)
                     /locus_tag="IWO38_000008"
     CDS             complement(11062..11913)
                     /locus_tag="IWO38_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ0312131.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            12079..13083
                     /locus_tag="IWO38_000009"
     CDS             12079..13083
                     /locus_tag="IWO38_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ0312132.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(13324..13680)
                     /locus_tag="IWO38_000010"
     CDS             complement(13324..13680)
                     /locus_tag="IWO38_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0312133.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            14048..15235
                     /gene="tuf"
                     /locus_tag="IWO38_000011"
     CDS             14048..15235
                     /gene="tuf"
                     /locus_tag="IWO38_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ0312134.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLTE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(15423..18014)
                     /gene="mprF"
                     /locus_tag="IWO38_000012"
     CDS             complement(15423..18014)
                     /gene="mprF"
                     /locus_tag="IWO38_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ0312135.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(18040..18759)
                     /locus_tag="IWO38_000013"
     CDS             complement(18040..18759)
                     /locus_tag="IWO38_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0312136.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            18968..19600
                     /locus_tag="IWO38_000014"
     CDS             18968..19600
                     /locus_tag="IWO38_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ0312137.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            19659..20519
                     /locus_tag="IWO38_000015"
     CDS             19659..20519
                     /locus_tag="IWO38_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ0312138.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            20626..23019
                     /locus_tag="IWO38_000016"
     CDS             20626..23019
                     /locus_tag="IWO38_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0312139.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            23201..25042
                     /locus_tag="IWO38_000017"
     CDS             23201..25042
                     /locus_tag="IWO38_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ0312140.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            25066..26175
                     /gene="rpoD"
                     /locus_tag="IWO38_000018"
     CDS             25066..26175
                     /gene="rpoD"
                     /locus_tag="IWO38_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ0312141.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            26395..28494
                     /locus_tag="IWO38_000019"
     CDS             26395..28494
                     /locus_tag="IWO38_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ0312142.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            28690..29553
                     /locus_tag="IWO38_000020"
     CDS             28690..29553
                     /locus_tag="IWO38_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0312143.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYGFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            29658..30131
                     /locus_tag="IWO38_000021"
     CDS             29658..30131
                     /locus_tag="IWO38_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0312144.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            30227..31114
                     /gene="xerD"
                     /locus_tag="IWO38_000022"
     CDS             30227..31114
                     /gene="xerD"
                     /locus_tag="IWO38_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ0312145.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            31133..31504
                     /locus_tag="IWO38_000023"
     CDS             31133..31504
                     /locus_tag="IWO38_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0312146.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            31474..32271
                     /locus_tag="IWO38_000024"
     CDS             31474..32271
                     /locus_tag="IWO38_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ0312147.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            32255..32824
                     /gene="scpB"
                     /locus_tag="IWO38_000025"
     CDS             32255..32824
                     /gene="scpB"
                     /locus_tag="IWO38_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ0312148.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            32830..33546
                     /locus_tag="IWO38_000026"
     CDS             32830..33546
                     /locus_tag="IWO38_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ0312149.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     regulatory      33628..33753
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            33876..34487
                     /locus_tag="IWO38_000027"
     CDS             33876..34487
                     /locus_tag="IWO38_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0312150.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     gene            34865..36388
                     /locus_tag="IWO38_000028"
     CDS             34865..36388
                     /locus_tag="IWO38_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ0312151.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            36625..37965
                     /locus_tag="IWO38_000029"
     CDS             36625..37965
                     /locus_tag="IWO38_000029"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ0312152.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            38118..38855
                     /locus_tag="IWO38_000030"
     CDS             38118..38855
                     /locus_tag="IWO38_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ0312153.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(39020..39241)
                     /locus_tag="IWO38_000031"
     CDS             complement(39020..39241)
                     /locus_tag="IWO38_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ0312154.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            39272..40303
                     /locus_tag="IWO38_000032"
     CDS             39272..40303
                     /locus_tag="IWO38_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312155.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            40290..41693
                     /locus_tag="IWO38_000033"
     CDS             40290..41693
                     /locus_tag="IWO38_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ0312156.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            41759..42394
                     /locus_tag="IWO38_000034"
     CDS             41759..42394
                     /locus_tag="IWO38_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0312157.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            42443..43123
                     /locus_tag="IWO38_000035"
     CDS             42443..43123
                     /locus_tag="IWO38_000035"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ0312158.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            43258..44487
                     /gene="rpsA"
                     /locus_tag="IWO38_000036"
     CDS             43258..44487
                     /gene="rpsA"
                     /locus_tag="IWO38_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ0312159.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            44669..45979
                     /locus_tag="IWO38_000037"
     CDS             44669..45979
                     /locus_tag="IWO38_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ0312160.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            46222..46497
                     /locus_tag="IWO38_000038"
     CDS             46222..46497
                     /locus_tag="IWO38_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ0312161.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            46732..47778
                     /locus_tag="IWO38_000039"
     CDS             46732..47778
                     /locus_tag="IWO38_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312162.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            47780..49039
                     /locus_tag="IWO38_000040"
     CDS             47780..49039
                     /locus_tag="IWO38_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ0312163.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            49045..49626
                     /locus_tag="IWO38_000041"
     CDS             49045..49626
                     /locus_tag="IWO38_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ0312164.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(49708..50583)
                     /locus_tag="IWO38_000042"
     CDS             complement(49708..50583)
                     /locus_tag="IWO38_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0312165.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            50731..51063
                     /locus_tag="IWO38_000043"
     CDS             50731..51063
                     /locus_tag="IWO38_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ0312166.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            51175..52383
                     /locus_tag="IWO38_000044"
     CDS             51175..52383
                     /locus_tag="IWO38_000044"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ0312167.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            52537..52992
                     /locus_tag="IWO38_000045"
     CDS             52537..52992
                     /locus_tag="IWO38_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312168.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            53354..53629
                     /locus_tag="IWO38_000046"
     CDS             53354..53629
                     /locus_tag="IWO38_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312169.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            53642..55720
                     /locus_tag="IWO38_000047"
     CDS             53642..55720
                     /locus_tag="IWO38_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0312170.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            55880..57766
                     /locus_tag="IWO38_000048"
     CDS             55880..57766
                     /locus_tag="IWO38_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0312171.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            57778..58725
                     /locus_tag="IWO38_000049"
     CDS             57778..58725
                     /locus_tag="IWO38_000049"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0312172.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            58745..59272
                     /locus_tag="IWO38_000050"
     CDS             58745..59272
                     /locus_tag="IWO38_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0312173.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(59335..59988)
                     /locus_tag="IWO38_000051"
     CDS             complement(59335..59988)
                     /locus_tag="IWO38_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ0312174.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            60120..60962
                     /locus_tag="IWO38_000052"
     CDS             60120..60962
                     /locus_tag="IWO38_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0312175.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            61120..62016
                     /locus_tag="IWO38_000053"
     CDS             61120..62016
                     /locus_tag="IWO38_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0312176.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            62019..62732
                     /locus_tag="IWO38_000054"
     CDS             62019..62732
                     /locus_tag="IWO38_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ0312177.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            62752..63270
                     /gene="msrA"
                     /locus_tag="IWO38_000055"
     CDS             62752..63270
                     /gene="msrA"
                     /locus_tag="IWO38_000055"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0312178.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            63267..63494
                     /locus_tag="IWO38_000056"
     CDS             63267..63494
                     /locus_tag="IWO38_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ0312179.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            63646..64197
                     /gene="lepB"
                     /locus_tag="IWO38_000057"
     CDS             63646..64197
                     /gene="lepB"
                     /locus_tag="IWO38_000057"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0312180.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(64280..64822)
                     /locus_tag="IWO38_000058"
     CDS             complement(64280..64822)
                     /locus_tag="IWO38_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0312181.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(65059..65457)
                     /locus_tag="IWO38_000059"
     CDS             complement(65059..65457)
                     /locus_tag="IWO38_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0312182.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            65717..66577
                     /gene="ylqF"
                     /locus_tag="IWO38_000060"
     CDS             65717..66577
                     /gene="ylqF"
                     /locus_tag="IWO38_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ0312183.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            66570..67337
                     /locus_tag="IWO38_000061"
     CDS             66570..67337
                     /locus_tag="IWO38_000061"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ0312184.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            67393..68250
                     /gene="dprA"
                     /locus_tag="IWO38_000062"
     CDS             67393..68250
                     /gene="dprA"
                     /locus_tag="IWO38_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ0312185.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            68372..70450
                     /gene="topA"
                     /locus_tag="IWO38_000063"
     CDS             68372..70450
                     /gene="topA"
                     /locus_tag="IWO38_000063"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ0312186.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            70419..71795
                     /gene="trmFO"
                     /locus_tag="IWO38_000064"
     CDS             70419..71795
                     /gene="trmFO"
                     /locus_tag="IWO38_000064"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ0312187.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            71993..72898
                     /gene="xerC"
                     /locus_tag="IWO38_000065"
     CDS             71993..72898
                     /gene="xerC"
                     /locus_tag="IWO38_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ0312188.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            72935..73483
                     /gene="hslV"
                     /locus_tag="IWO38_000066"
     CDS             72935..73483
                     /gene="hslV"
                     /locus_tag="IWO38_000066"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ0312189.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            73497..74897
                     /gene="hslU"
                     /locus_tag="IWO38_000067"
     CDS             73497..74897
                     /gene="hslU"
                     /locus_tag="IWO38_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ0312190.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            74915..75706
                     /gene="codY"
                     /locus_tag="IWO38_000068"
     CDS             74915..75706
                     /gene="codY"
                     /locus_tag="IWO38_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ0312191.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            75788..76663
                     /locus_tag="IWO38_000069"
     CDS             75788..76663
                     /locus_tag="IWO38_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ0312192.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(76795..77433)
                     /gene="plsY"
                     /locus_tag="IWO38_000070"
     CDS             complement(76795..77433)
                     /gene="plsY"
                     /locus_tag="IWO38_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ0312193.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            77560..77994
                     /locus_tag="IWO38_000071"
     CDS             77560..77994
                     /locus_tag="IWO38_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ0312194.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            78160..80205
                     /gene="parE"
                     /locus_tag="IWO38_000072"
     CDS             78160..80205
                     /gene="parE"
                     /locus_tag="IWO38_000072"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ0312195.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            80217..82667
                     /gene="parC"
                     /locus_tag="IWO38_000073"
     CDS             80217..82667
                     /gene="parC"
                     /locus_tag="IWO38_000073"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ0312196.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            82944..85175
                     /gene="pflB"
                     /locus_tag="IWO38_000074"
     CDS             82944..85175
                     /gene="pflB"
                     /locus_tag="IWO38_000074"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ0312197.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            85419..86180
                     /gene="pflA"
                     /locus_tag="IWO38_000075"
     CDS             85419..86180
                     /gene="pflA"
                     /locus_tag="IWO38_000075"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ0312198.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            86265..87191
                     /locus_tag="IWO38_000076"
     CDS             86265..87191
                     /locus_tag="IWO38_000076"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ0312199.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            87373..88266
                     /locus_tag="IWO38_000077"
     CDS             87373..88266
                     /locus_tag="IWO38_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ0312200.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            88400..88525
                     /locus_tag="IWO38_000078"
     CDS             88400..88525
                     /locus_tag="IWO38_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0312201.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(88659..89291)
                     /locus_tag="IWO38_000079"
     CDS             complement(88659..89291)
                     /locus_tag="IWO38_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312202.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(89439..89981)
                     /locus_tag="IWO38_000080"
     CDS             complement(89439..89981)
                     /locus_tag="IWO38_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ0312203.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            90116..90505
                     /locus_tag="IWO38_000081"
     CDS             90116..90505
                     /locus_tag="IWO38_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312204.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            90509..91273
                     /locus_tag="IWO38_000082"
     CDS             90509..91273
                     /locus_tag="IWO38_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0312205.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            91402..92349
                     /locus_tag="IWO38_000083"
     CDS             91402..92349
                     /locus_tag="IWO38_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0312206.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
BASE COUNT        31424 a        15925 c        19418 g        25599 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag
       61 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
      121 tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt
      181 tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct
      241 tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg
      301 tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag
      361 cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc
      421 agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga
      481 tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag
      541 gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt
      601 ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt
      661 ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg
      721 gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt
      781 ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc
      841 agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg
      901 tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac
      961 caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca
     1021 atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt
     1081 tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata
     1141 ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga
     1201 tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat
     1261 cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc
     1321 ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat
     1381 cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta
     1441 attctttcaa gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga
     1501 aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc
     1561 aaatggctgc tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta
     1621 atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata
     1681 acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct
     1741 ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag
     1801 gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat
     1861 atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac
     1921 aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa
     1981 atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga
     2041 tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga
     2101 aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt
     2161 ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac
     2221 tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt
     2281 tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga
     2341 aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg
     2401 caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg
     2461 atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt
     2521 cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta
     2581 caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt
     2641 caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc
     2701 aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt
     2761 tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta
     2821 caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag
     2881 ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata
     2941 aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga
     3001 caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg
     3061 actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag
     3121 ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag
     3181 acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa
     3241 accagcctta tcaggaaatc cgaaacaact acccaagaca agcagaactt ccttttgact
     3301 cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta
     3361 aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg
     3421 ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac
     3481 gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg
     3541 aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg
     3601 aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg
     3661 gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa
     3721 acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt
     3781 tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg
     3841 cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag
     3901 cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg
     3961 cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag
     4021 gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag
     4081 gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat
     4141 tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag
     4201 aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta
     4261 cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct
     4321 ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa
     4381 cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta
     4441 tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc
     4501 tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg
     4561 gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa
     4621 tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt
     4681 gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt
     4741 gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt
     4801 tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg
     4861 aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa
     4921 agccaatcat tttattattc atcaaaagag atagaataga taaaagagga gaatgattta
     4981 ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat
     5041 gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg
     5101 aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg
     5161 ttgctggagg atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga
     5221 tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc
     5281 caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg
     5341 aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga
     5401 tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt
     5461 tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg
     5521 taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt
     5581 ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt
     5641 acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa
     5701 tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa
     5761 cctttcacga ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata
     5821 tcattttcga tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg
     5881 tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga
     5941 gaatctagca tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga
     6001 taacctgtaa aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct
     6061 cctggctgta gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca
     6121 gtgtaaagat taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga
     6181 cgttcctgat tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc
     6241 ttagtatacg gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat
     6301 tctttatgtc gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga
     6361 ccatcaacaa caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc
     6421 agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca
     6481 acagcaccga caccttgcgt cacttcacca ccgcttgcca agccgtaacg agcaatatca
     6541 cttgctatga ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc
     6601 aaagctgcgg tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg
     6661 attttcagta aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct
     6721 gttccgacga tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca
     6781 gcatttgctc ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga
     6841 ccaatcccta tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata
     6901 tctaaatata aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca
     6961 cctctttact tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa
     7021 tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa
     7081 caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg
     7141 aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta
     7201 agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca
     7261 aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat
     7321 ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca
     7381 atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg
     7441 atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca
     7501 cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg
     7561 gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat
     7621 gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag
     7681 ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag
     7741 atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa
     7801 aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct
     7861 tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat
     7921 aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt
     7981 atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat
     8041 ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt
     8101 agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta
     8161 ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt
     8221 cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca
     8281 gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat
     8341 cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa
     8401 actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa
     8461 aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc
     8521 aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat
     8581 ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa
     8641 atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa
     8701 gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt
     8761 gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat
     8821 tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt
     8881 gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt
     8941 aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg
     9001 agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg
     9061 aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct
     9121 gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg
     9181 gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt
     9241 ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca
     9301 aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact agcacaagta
     9361 attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga
     9421 atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt
     9481 ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag
     9541 acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga
     9601 ataaagcaag atttacagtt attttattta ctgcagatct tgcttttttc tctatcagaa
     9661 aatatgttta ttattagaat tttttatagt taaggttagg catcttaaga aatcaggcaa
     9721 ctccaagata tcttcaggta gaagagaagt cgcctaacat tgtaggatca taggtgattt
     9781 gttggacagg tcaagctgat atttgagcct gatgttagaa ggaatttctt tttacttgct
     9841 gtagaaatta ttatatgact aaaaaggctg ggattttaaa tcccagcctt ttatgacaaa
     9901 tagaattttt atcgatcagt aatcgctgtc gctgcaaatt gttcgatcaa aggaatcaaa
     9961 gtcaatttga ctttgtattt ccgttcgagt gctttttcga taacgaactc attttcagta
    10021 gtcatagaaa tagttgctaa tttacgaggt tcatcagcat ttttttcatt aataactact
    10081 tcatgttcat tttgttctgt gtaaaattta ggatactcag caacggcttt tgaaaaatca
    10141 gcaaaactta aagcggcatt cgtatcagtt gagcgtgtat tttcagttgt gtccgctttt
    10201 ggtttttccg ttttttcttc tggcacactg actgtttctt ctgtaactgg tgtcgcaaca
    10261 gcttgcgcag ttggttcatc gtttttcaat cgttgatcat tccagtaaga cacgatttca
    10321 ataagaagat tgtcacggaa tcgttcgaaa atcccgtttt ctcctgaacg aattctcttt
    10381 aatacagtat tgaagttgtc atcgattggc acgttgatcc gtgtatcttc atttgttttc
    10441 ccagctaatc cgatcgttaa gatttgacgg tttttgggat caagcatgac ttgtaacggc
    10501 aaaccataac gtcgcaagct ctcttcttct agagaacgga ataattgtcc tcttacacca
    10561 tccaatgaag gacggaattg atttaataat ttttgtttct gttttttctt catatgttct
    10621 ctccttcgct catacccttt cgattataag aaaaaaacag ccaaatgaaa aggagtaatg
    10681 caaaaaaact tgcgtatacg caaattacaa cagaaaaatt tgcgtatttt cccgtttttt
    10741 gaaagaaaaa taaaaaatgg aacggattat gtatatatac ataatccgtt ccattctttc
    10801 tattttttcg gtatcaaggc aaaaacaggt agttcaggtg aataaatgac ttcatgttcg
    10861 tgccagggtt tgaccattag cgaatgatcg aaccgttttt tcagttcttc gatgatttga
    10921 tcatgtgtcc agtttcttgc aggaaaatgt tggattttct ctgcatcgat caaaaagaca
    10981 aaatcggaat gaaaagtctg ggtaacttct tccgagagag aattcaagcg ttcgattgga
    11041 tccataaaat cacctttcga cttaaaattg ggtagtatct gggtcttttg ctttgccgac
    11101 tacaccatct aactgatcga tttgtttcat ttcttcttct gttaattcaa aatcaaataa
    11161 ttcagtattt tctttgattc tgcttggtgt cactgactta ggaagaggaa ggaattcgtg
    11221 ttgcagcgac caacgcaaag caacttgtgc aatcgtcttg tcatgtgctt cagcgatttg
    11281 tttcatttca ggcacatcga agattttacc agtgcctaat ggactatatg cttcgaggat
    11341 catatcatgt ttttttgcat actcaactac agttgtctgt aattcgcctg gtgcaaggaa
    11401 aacttgattg accatcggca taatttttgc tgtttttgct agtgtatcta gatggtgggg
    11461 taagaaattg ctcacaccga ttgctttgat tttacctgct tcatacagtt cttcaaatgc
    11521 tcgccatgta tctgcatttg cttgttccca attatcacga aaagcaacag ggtttggcca
    11581 gtggatcaaa aacagatcta aataatctgt ttggagcttc ttcaatgatt cttcaaaaga
    11641 agacatcaca agttcatagc tgtggtttgc attccataat tttgttgtca agaatatctc
    11701 ttctctagga attccacttt ccttaatcgc ttggccgacg ctttcttcat tcttgtatcc
    11761 ttgggcagta tcgatatggc gataacctgc agccagcgct tctttaacag aggaaacagc
    11821 gacatcccca tcaggtgttt gccatgtgcc gaatcctact accgggattt cgtaaccatt
    11881 tgatagacga taagtgcttg ttaatgaatt catactttca gctccttttt tattcgttca
    11941 actataagtg tagcgcattt ttcgaaaaaa agaatccatt ttgtttctac tcttgaaatt
    12001 tttgtttaaa agaaaatgat tttgattctg gtatttttga ttttttttcc gtaaactaag
    12061 acagaaggga atgagaaaat ggatatctat ttaaaaaaag caattctgca tattattgac
    12121 agagaaactg gttctccagt cttttcacaa aaagagctgg atctgacaaa agaatatatt
    12181 cgtgattttc ttcaaaagaa gatccaaaaa atctcttctg ctcaaacaaa aactggacaa
    12241 ctagcagaag aaagcatgat cgttgaatca cttaaaatcg ctcaagaaaa ttttgtggaa
    12301 gcaagtgaag caatcgttca gcggtggttc gatatctacc aagaaagtga agaagcacct
    12361 agtgcagatg tatttgttgt cttgtatgaa ttagatacag tgatgtactt atctttattg
    12421 aaagtcaatt atcgtgaggc ttacactcac tttgttgaag cagacgaagc agggatcgac
    12481 aaccgcttga ttttgaatcg agcaattcta ggggcgaaaa cacaaaaagc agatgaagca
    12541 ttcgctgtga atttgactga ttggacctac gaattaattg aaaaaaaata tgagttttct
    12601 ggcaaaaaag agctttattt ttctggaaga gtgatcgaaa gtgttcctgc accttcttta
    12661 gaagaaaatg taaaagtgat ccaaaaagta gccaagcatc ttggaaaaaa atttgagaca
    12721 gaagaatttg atatcatggc tgatttgaaa gaagctgtct atgatacgat agaagaaaaa
    12781 ggtcggttgg atcatgagat gattgcggaa aaagtcttca aggaaaacat cacggcaaaa
    12841 atggctttta aagaagaagt acaagagcaa ggatttgtcc cagaagcacc tcctgtaaaa
    12901 gaagtacaag aaatttctga gaaaaaattt ggaaaacaaa aattcaagct atcaaacggg
    12961 atcgaactga tcgtaccagt agatgtatat cgcaacccag atttgattga atttgtgaat
    13021 aatccggatg gtacgatatc cgtgatgatc aaaaatgtgg atgaagtatt gaatcgttta
    13081 taaaaatgac tagaatatta ggtgttcaag taccagtgat cagttaaatg aattgtgtca
    13141 agagaaggat cggaaaagac gaatataaca aaaaaagagc gtgagacaaa agtaaagaac
    13201 acttttgtct cacgctctaa acacgtataa acggcggaag cagaagcaac tccttcttat
    13261 ttcttggagt taaacgcttc tgtcccagcc tctttttatc taataaaatt ttaagaccat
    13321 ttactaatta tcgatttttt ctgccgcatt tttaggcact tcagaagcct ttatttcttc
    13381 ccagcttaat acacctttac gaggattgac tttgagttta agatacgcat tcatctttaa
    13441 tggatgttct ctttcttcgc gaagcgtcac ctgttttttt tcaccatctt tattataagc
    13501 cgcttgcttg taggtataag tcgtgaattt ctctccgtta tctgcttggg tagctgacat
    13561 ttcgccattc gtcgtgattt ttgtataata agcttcgccg ccgtaaaaat aatgataagc
    13621 tgcaaaactt ccgccgagaa atatgcctaa aacgacaagt acgccaatta attttttcat
    13681 gtacttcacc tctttcctta ttattaacga aatagaagat ttcgtctatt gaaagttgtt
    13741 ggcaacagct gaattttaaa cttttcctca ttttgttctt attgcttcaa aagaaagtta
    13801 aggactatct tgaagttttt ctcaaaaaac acattttttt atttataaaa ggtacttttt
    13861 tcgcgtataa gacagacgag tgctcttagt tgactttgac caatattgac gtataataag
    13921 cttagttcga atcttacaaa aagtaagtcg gacgtattct ttttgattta gtcaaaaaaa
    13981 gactataata aaaaggtgag agctgaaatt tttcagtgat ctatattatt taggaggttt
    14041 gtaaattatg gcaaaagaac attatgacag aagtaaacca cacgttaaca ttggtacgat
    14101 tggacacgtt gaccatggga aaactacttt aactgctgcg atcactacag tattaggcaa
    14161 aaaaggccta gcaaatcctc aagactatgc tagtattgat gcagctccag aagaacgtga
    14221 acgtgggatt acgatcaata ccgcacacgt agaatatgaa acagaaaaac gccactacgc
    14281 gcatattgat gcaccaggac acgcggacta tgtgaaaaac atgattacag gggcagcaca
    14341 aatggatggt gcaatcttag ttgtttctgc aactgacggt ccgatgcctc aaacacgtga
    14401 acacatttta ttgtcacgcc aagttggtgt aaaatacctg attgttttct tgaacaaagt
    14461 tgatttagtc gatgatgaag aattgatcga tttggtagaa atggaagttc gcgagttatt
    14521 gaccgaatat ggtttcccag gcgatgacac tcctgtgatc aaaggttccg cattaaaagc
    14581 attgcaaggc gatccagatg ctgaagctgc tattatggaa ttgatggata cagtagatga
    14641 atatatccca acaccagaac gtgatacaga taaaccatta ctattgccag tggaagacgt
    14701 cttctcaatt acaggtcgag gaactgttgc ctcaggtcgt attgatcgtg gtgctgttcg
    14761 tgtcggtgat gaggtagaga tcgtagggat caaacctgaa acacaaaaag cagttgtaac
    14821 aggtgtagaa atgttccgta aaacgttaga ttacggggaa gctggggata acgtaggcgt
    14881 gttgttacgg gggatccaac gtgacgatat cgaacgtgga caagtacttg ctaaaccagg
    14941 ttccattact ccacatacaa aattcaaagc agaagtgtac gtgttgacaa aagaagaagg
    15001 tggacgtcat actccattct tcaacaacta tcgtccacag ttctacttcc gcacaactga
    15061 tgttacagga acaatcacat tgccagaaga tacagaaatg gtcatgcctg gtgacaacgt
    15121 gacgattgat gttgacttga tccatccaat cgctgttgaa aacggaacga cattctcaat
    15181 tcgtgaaggc ggacggacag taggttcagg tatcgttaca gagatcgaag cataagaaac
    15241 gcgactcgaa tattttggta atgaaaaatg tttaaaataa aagagcggtc caaacaggat
    15301 ttctgtttgg accgctcttt gttcttcaat tgtataagaa gagattgtag aagcatgaac
    15361 tttcgtttta cttcagtctc ttttattcat catgagagtg ttcatctatc ggtatgcagt
    15421 ttttaatact ttcttcgtat ccattttttc aaaccactta ttctaccttg tgtctgctca
    15481 actggcgcat tatcaataat cagcaaagca atcataacat atgcaatcca actatctcta
    15541 gaatatagag tataacgtga gacccagttt gtagcgtact tgcttttata ttctcttaat
    15601 ccatggaaag aataaaaatg agaaccaaac tcgtaaacca agtatgcaat cctttcctgt
    15661 aaaaaactct ttctggaagt tccgacattt gaaagagggg ccatccctaa attgaaccat
    15721 tggatatgtt cttctttcat ataggcaaac aaatgcaaga acaagaaatc catactgcca
    15781 gaaggtgctt ttttagaatc atatcgcatc agatcaatcg ttcctacttt attgttcgtg
    15841 taagtaggca tgatcgttgc aaaagcgaca atttcttctt gggtattttt gacaactgca
    15901 ataggcgctc tttgtagata atcttcagag aaaaagccta aagaaaaccc tttttccttt
    15961 cgtgaaccca accaattatc agagatattt ttgaaagtac tcatttgctc agtagaaaat
    16021 ggtggttgta agacatcaaa tgtaaatcct tctcgttcga tccgattcaa gacagcacga
    16081 gtgcccttca tttttttccc tgaagttgta aaactattca ggtctacata agcttcttcc
    16141 cccatcttaa tgaaatcgta gccgaattca tgcaaaatca taacaatctc ttcgcttgtt
    16201 tcgtagaaaa ctggtaaata gcatagtcga tctgtttctt cgataaaagc ttcgattgct
    16261 tctggaaaat cttctttctt accagaagga tcacccatta caatacattt attattgaag
    16321 caagcgaatt ggagaagaac agttggttca ccgtcttttt cataggcaaa catccgctta
    16381 tcttttaaga agatcaattg actatcggag tttcctccat aagttgtaag gatcttcagg
    16441 gccttttctt cattaaaggc ttcgccaatc tgtttctttt ctccttgaag gaaatgaaca
    16501 aatagaacga tcatgaaact aacagcgatg atggccaaca atcccgaaaa ccaaatcttt
    16561 tcagaaggaa ataagaaaaa ggaaataaaa tggtgacgcc gatgagggaa atctggcaga
    16621 ttgtataccc caataacgat gtaaagcaag gacagtgcac caataatgat tccatcgatc
    16681 gtacgccact cccatgaata gatcaattgt tcccgaaata attcgttttt agaagcaaga
    16741 atgatcagaa gcagaataga caagaaaacc accggtgtga aactaaaatc acttaataaa
    16801 acataaagta aagctagaac aatcaagaag attgtaggca aatatgctcg tttcacacgt
    16861 gctgcgatcc ctctgcccat aatgattaaa agaaatccca atagtatact aggaaattgg
    16921 ataataaaat gaaatttcaa tgggttcaaa ctatgaagcc agcgaaactc tgtaaaagct
    16981 tgcgggatag tggcagaaag tactagcatg attccagaaa agtataataa gacaaccacg
    17041 attttatgcg caatttctgt tgctaattgt ttagggatac cagaataacg ctgatcaaaa
    17101 gtggaaccga tatttttgaa gaaaaagacg attccaatca aaaacggaat gatgtagtag
    17161 aacaggcgat agagcaagat ccacacaacg actatttctc ttggcacacc aatagcggac
    17221 aacccgataa tcatcattac atcgaaactc ccaatttctc ctggaatcat agacacgatt
    17281 cctatcactg aagcagcaat gaataatgga attgtttgcc acagatcaat ccggatttcc
    17341 attaaataac caacagaaag gaaactaaac aggacaccgg tccattccaa caatgaaact
    17401 agcaatagct gtccttgagt tttaagtgag gtaccaccaa ggaatccttt ctttttgaaa
    17461 gtagtaaata aaaagacgac aggaaaatat agaccgccgc cgatcaacca tatccaatat
    17521 tgcttcaaaa aaggagcaac tggcgtgaag actacaagta aaaaagaaag cagactgtaa
    17581 atagaaagtc cagccatcaa aaacaagaaa actttcgata atgcttggat cacgtttctg
    17641 ctctctttct tttgtccata tagttcagag cgaagcccca cactaacaaa cccgccgaaa
    17701 ccggcaatat tgttgatcgt attgatcagc cagcttgtct caaacaggta acgaggattc
    17761 tgcttttgtt caagcatccg attcaaaata atatcataac caaccatagg taacacagag
    17821 ataagaccaa tcaatagcat tagtccgctt ttccatacag gtatagtggc aaacgtttct
    17881 cctaattgct ggatagatag agttttaccg atggacatca attcaccgac aataatgaca
    17941 atgacagaaa tcaaaaagat tgttttgaac agtcctaggt ggtttttcag ccattgtatg
    18001 gtatttttta acaaataaat caccttctat tcctaatagt tatgtttcag aaagaaattg
    18061 aagaacttct cgattgaagc gttcaggatc ttttctagct aattcatgtc cttgttcttt
    18121 cacaagcaca aaggaagctt ttggtatagt tttagctata tacaaagaat gttttagctt
    18181 gattacatct ttttttccca cgatgatcaa agtgggggag ttgatttttt ttaaatcatt
    18241 ttctgtaagc ccgatgtcat gcagcaaaag tttgataact aaaagatttt tgcgaagact
    18301 tgggcgaaat aaagagagta accataccca tgcataatga atattactga tgactctggc
    18361 agaaaaacga acgcctttaa caagcgtgtt tccagaattt aaaatcaatc gatgaacttt
    18421 tttagggaag ctgctggcaa ataccagtgc tagatttgct ccatcactaa atccaagaaa
    18481 atctgcctga tcgatttttt ccagcaacat gatcgtattc agatcctcgg ccattaactg
    18541 aaaattcaac atagatgctt cgtttgtcga acgaccatgt cctcggctat ctactagata
    18601 cacggtatag tagcgttcga gtacagggac ctgttctgaa aaaaagcggc cgctgccgtc
    18661 attgccatgc agtaaaaaga gagggaagcc ttgaccgctc ttttcatagt aaatttcgct
    18721 cccatctggc atttttgcat atttcttttc ttttttcaca taatcaccta ctgtgtaaag
    18781 tataacgatt atatcagtca ttgggcaatt gtttttactg aaaaacaacg atcattttcg
    18841 taagatgtca aaaaatacag gaaaattcat tttttcttaa ggcgtacaca gaaatcaagg
    18901 aagaaatagc caagaagata aagaaatagt aagataaaaa aagatgtcaa aataactagg
    18961 tgatgaaatg aagcgaaaac gattatctat tatccgtatt cttttttaca tgatcattct
    19021 gacggttatt ggtgcggtgt tgtttttagg aggaagaact tatcgttcgg taaaacgagt
    19081 ggaaagctat caaatagaaa taacaacagc tgcaaaaaat tatcatatgg aaaactacga
    19141 gaatttgatc aaggcgataa tattgaccga atcaaaagga aaagggacag atctcatgca
    19201 gagctcggaa agtctgcatg gagaagctga gatcattgat aatccagctg acagtattga
    19261 tcaaggtgtc aaatatttgg cggaaatgat agtagaagca aagaaacaag gatgcgattt
    19321 atggacagct gtccaagcat ataattttgg gaaagattat attgcctatg taaaaaatca
    19381 tggcggagaa aacacagtag aattagcaga agcgtattct aaagatgtgc tgagcccact
    19441 tttaggaaat gaggaaggga aacaataccg atattggcgg atccagtcct tgctttacaa
    19501 tggcggctat ctctatcata atggaggtaa catgttttat cctgatattg tcaaaatgaa
    19561 tgaaaaaata atagagacct atgaaaaagt ctttaagtaa aatagcagga aatgttcaat
    19621 catgaataaa attaaatgaa agaaggagtg cgcaaatagt ggaaaaaagg caaaaaatgg
    19681 aaaatcaaga agtggggtac acaaccaaag actggatgtt acggtttgtg aagggaatgt
    19741 ttatcggttc aggatttatc ttgccaggag tcagcggtgg tgcattagca gctgtctttg
    19801 gcatgtatga gcggctgatt tcttttttag cccaccttac aaaaaatttc aaagaaaatg
    19861 ttttgttttt tctgcctgtt ggtctaggcg gagtgacagg catctttcta ctttcttttt
    19921 tagtcagttt tttattaggt gcgtatgaaa caacgatatt atggtttttt gtcggatgta
    19981 tcgctggaac tgttcctgcg ttatggaaag aagcagggaa aaaaggaaga gatacttctg
    20041 atgtgatcat catggttgtt acttttgttc tggcttattt gtttttatta tacggcgctc
    20101 aattatttga cgggcaagtc gatcagaatt ttggaacatg gctgattgcc ggcggattga
    20161 ttggtttggg tatgatcgtc ccaggattga gcccttctaa ttttttggtg tatatgggat
    20221 tgtataaagc gatggctgac ggatttaaag aagtacgact tgaagtgatt gtgccgatcg
    20281 gaattggtgg gattctttgt gttcttggat tgtcaaaaat catggattac attttctcaa
    20341 aggcatatag caagctattc catttcattt taggtgtagt ttttgcttca acgattatga
    20401 tcattcctac cgattacgcg ggattgggag ttgctggggt actagcttgt cctgttcttt
    20461 ttatagccgg tacagcacta ggttggtgga tgagccgttt ggaggaacaa tataagtaaa
    20521 gatagattga taataaaaac agcacacggg tgattcaaac aaaaaggttg gcatcttttt
    20581 tgtaaaatca ttcgtgtgtt tttctataaa ttttataaaa acaagttgtt tttaatcttg
    20641 gaacacgcta gacttaaaca cgtgattatt tttgaaagtt ggcggaaaat ggagaaaaag
    20701 aaaatgaatg gacttacaca gcaagaggta gttcagaaaa tggcttctgg ccagcaaaat
    20761 gactaccagg aagatgcatc aaaaagtaca aaacaaatat tcagcgataa tatattgacg
    20821 ttattcaatt ttctgaactt tggtattggg atttgtctgt tatttgtagg tgcgtattcc
    20881 aacatggcgt atttagcaat tattgtcgtc aatatcgtta ttggaattta tcaagaaata
    20941 catgcaagaa atcttgtacg gaaattatcg attgtttccc aatcagaagt actagtgatc
    21001 cgggacggta aagaacaatc gatttctact aaagagctgg tattaggaga tttggtaaaa
    21061 atcggagcag gagaacagat accagccgat ttacaggtta tatctggacg ggcagaagca
    21121 aatgaagctc ttttgacagg cgaatctgat ttgattgtca aagaaactgg agctgaattg
    21181 ctttctggaa gctttttgac aagcggttct gtcatggctg aagttatccg agtaggtgca
    21241 gataattatg ctgtccgctt gactagcgag gcaaagactc acaaagctat tcgttctgaa
    21301 cttgtagatt cgattcgcaa agtatccaaa ttcactagtt tcgtgattat tcctttgggg
    21361 atcatactat ttttggaagc actgttcatc cgcgatgctt ctatcaaaat ttctgttatt
    21421 gcatctgcag cagctttgtt aggcatgctt ccaaagggtt tggtattgct aataagtatt
    21481 gcattaacga caggggtaac caaactggca aaaaaacgaa tccttgttca agacatgtat
    21541 tcaattgaaa cgctggccca tgtcgataca ttatgcttgg ataaaacagg gaccattact
    21601 gaagggaaaa tgagtatcca gcgggtagaa gcactacatc cgctgtacga gaaagccatc
    21661 ccagaaatta tggggacgta tctggcagaa agcagtgata acaacatcac tatgcgggct
    21721 atgagagatt attttgagct ttctaatcgg tatgatgtga caaacaaagt cgcattttct
    21781 tctgatcgca aatggggcgc gatggaattt ccagaactgg gagtcgtgta cgtaggagcg
    21841 cctgaacgtt tggtggaaga agatcggctt ccggacagtc taaaaacagc ccaagcgaat
    21901 ggctatcgcg tacttatgtt ggggatcgct ccaaatcagc aattgaacga agaaaaacca
    21961 gcatttgttg agccggttgc tctgtttgaa atcgatgatc caatccgaaa aaacgctcaa
    22021 gaaacactag cttatcttca tgaggaaggt gtggatctga aagtgatttc tggagacaat
    22081 ccttttactg tctccaatat cgctagacgt gcaggattac cgaattatga tgcgtatgtc
    22141 gatctttctc aaattactga agaaatggaa gtgcgagaag ctgctcatcg ctatacggtt
    22201 tttggacgag tgagtccgca gcaaaagaaa ttgctggtaa atgaattgaa agaaagcggc
    22261 agaacggttg caatgacagg cgacggtgtg aatgatgttt tggcgttaag agaagcagat
    22321 tgcagcattg ctatggcaga aggtgatgga gcaacacgtc agattgcgaa tctggtattg
    22381 ttggactctg attttactac attgccagaa gtcctgtttg aaggacgccg agtggtcaac
    22441 aatgtgacga aagtctcagg catctttttt atcaaaacga tttattcatt tattttatcg
    22501 attatatgtg ccgttacagc aattggattt ccgtttatcc ctatccaaat cacgctgatt
    22561 gatttagcga ttgaagggta tccttccttc tttttatctt ttgaacaaga taaaagaaaa
    22621 atcacttacc gttatttgcc tacagcactt attaatgctt taccaaatgc cttgcttgta
    22681 gtagtgaata tcattgcggt gtatctactt ggaaaaacat tcgcatgggg aaatgctgaa
    22741 actactactt tgatgtatta cttgctgatt gggatcagtt gcatggcagt tgtaaaagct
    22801 tgttatccct tcaacccatt gcgcatcttc ctagcagtaa cgacgatcgt agggatatat
    22861 gtagctgcaa tgctgttcca tggaattttg gaagtaaact tagggttggg agagagcgta
    22921 ggatacttct ttgtattcat gctcgtaaat attgttcttc gagtattgta ctggaaggct
    22981 gatattcaaa atcagttact gaaaagagta gcaaattgat gcttaatgag atagaaatgt
    23041 gttatacttg aaaaagcaat gaaatatgat acaatcaaac atgtataatt gtgtccttta
    23101 aaatcgcact gatttcagtt agtgcgattt tttaccggtt attctcctga aatatcttct
    23161 gttaaaccca aaagatatca atagagcagg gggtgaaaaa atggctcaac ggattcctca
    23221 agaggttatc gaaacagtac gcagtcaaac gaatatcgtt gaggtgattg gacaatatgt
    23281 ccagttgaaa aagtctggta aaaattactt ggggctgtgt ccttttcatg aagaacgtac
    23341 accgtctttt tctgttgcag aagacaagca gatttttcat tgctttggtt gtggtaaagg
    23401 cggaaacgtg tttacttttc ttcaagaact agaaggtcta agttttccag aagcagtggt
    23461 aaaagtggca gacttcgaac agataccgtt agaagatcaa tggcgtcagc gtactatagc
    23521 tgtccaaaat cctgaatctg ctacggggaa actgattgct gctcatgaaa aagcagcgga
    23581 aatctatcat cacatgctat tgcatacaaa agctggacag ccagctttag aatatttgca
    23641 aagtcgtggg ttgtctttag aattaattga agaattcaac ataggttttg cacctaatga
    23701 gcgttctttt cttcagcaag tattcaagaa tgaatcaatt tcaaatgaat tgttagaacg
    23761 ctctggattg tttgtcacgc atgatgatgg ccgtatgtct gatcgttttt atcaaagagt
    23821 gatgtttcca cttcgtaatt ctcaaggcca gacgatcggt ttttcaggac gctggcttgc
    23881 acctgaaaaa gggcaggaaa aagatcagcc caaatacttg aatagtccag aaacagaact
    23941 gttcaataaa agacaagttt tatttaatct tgataaagca agaagcgata tccggaaaaa
    24001 cggagaaatt ttgttgttcg aagggtttat ggatgtgatt gctgcatggc aggctggtat
    24061 caaaactgga ttagcttcta tggggacaag tctaacagct caacagatcc aacaaatgga
    24121 aaagctgacg aaagaattgg tcttttgcta tgacggagac aacgcaggtt ttgaagcaac
    24181 caatcgaggg attgaattgc ttcggcaaaa cagtcgatta gcactttctg tcgtagtcgt
    24241 tccagaaaag cttgatcctg atgaatatct ccgtaagtat ggagaagcat ctttccaaga
    24301 attagtggct catgggcgcg aaacagtctt tacttttaaa tcacgttatt taaaacaagg
    24361 gaaaaattta gataatgaaa aagacaagat cgaatacctc gaagaattac tagttgaact
    24421 tagccgagtt gtttcaccaa tcgaacaaga tatgtatcta tctcaactgt caacagagtt
    24481 tcagatcggc agagagacga tacaaaaaca actgcgcgat cttcgaagga caaatcagca
    24541 aaatcgtcaa gaacctcagg aaagtcaaca cactcaggtt acgagacaag aaactgcacg
    24601 gaaaaagaga cctttgacgc aagtagaaaa ggcagagcag attctattat atcgtgcatt
    24661 taaagaagtt tctgtccgta atattttaaa agaacgagaa tttcagttta ttcatgatgc
    24721 atatgcagaa gtttattttt tattcgatgc atatgtctca caacattatg aattcaacct
    24781 tgcagagttt ttagactttt tgcaagatga aaatttaagg cggttggtgg tggaaatcga
    24841 atatttaccg gtggcagaag aaagttcgcc acgagaaatc caagacttgc tagatgtgat
    24901 cagcaaatct ggcttagctg atgaaataac attaaaaaaa caaatgcagc aagaagccag
    24961 acgaactgga aacaaacagc ttgagcttga attgacgatt gaaatcatca atttaacgaa
    25021 acagttaaaa caagccaaat agctactcaa ggggggcctt cttttatggc aaatggaaca
    25081 gataaaaaaa cgtatgaagc agcagtagca gctttcatca aagaaaacaa acccaaacga
    25141 caagttgtat atgatgattt atccaatcag cttgctacac cattcacttt gaatgcagat
    25201 gaaatggaca aattgatcca aaagattgaa gacgcaggta tcagtgtggt ggatgaaaat
    25261 ggtgatccat caattcacag cttgaagagt gctgagaaaa aagctgaaca agcaaaaaca
    25321 gaagatcttt ctgcgccaac cggtgtaaaa atcaatgacc cggttcgtat gtatctgaaa
    25381 gaaattggcc gtgtctccct tttaactgcg gaagaagaag tagcgttagc attgaaaatc
    25441 gaagaaggcg accaagaagc gaaacagcgt ctggcagaag caaaccttcg tttagtagta
    25501 agtatcgcta aacgttatgt aggacgaggc atgcagttcc ttgacttgat ccaagaagga
    25561 aacatgggac tgatgaaggc agttgaaaaa tttgactacc gcaaaggatt taagttttca
    25621 acctatgcta catggtggat tcgtcaagcc atcactcgag caatcgctga ccaagcacgt
    25681 acgatccgta ttccagttca tatggttgaa acaattaata aattgatacg tatccaacgt
    25741 caattgctgc aagaccttgg aagagaacct acgccagaag aaatcggtgc ggaaatggat
    25801 cttccaacag aaaaagtccg agaaatcttg aaaatcgcgc aagaacctgt ttcccttgaa
    25861 acgccaatcg gtgaagaaga tgactctcac ttaggtgatt tcatagaaga ccaggaagca
    25921 acaagtccag cagaacacgc agcttatgag ttactaaaag aacaattgga agatgtacta
    25981 gatacattga ctgatcggga agagaatgtc cttcgcttac gttttggttt agatgatggc
    26041 cggacgagaa cattggaaga agtagggaaa gtgtttggtg ttactcggga acgtattcgt
    26101 cagatcgagg ctaaagcatt aagaaaattg cgccatcctt ctcgttcaaa acaattaaaa
    26161 gatttcttag aataagcgtg gaccattgac cagaagagaa atcagtaaaa ctgtttggtt
    26221 tattttaaaa gaaaaaaagc ttgagactac gcagtctcaa gctttttttt ctataggaag
    26281 gaaattcatt cattattttt aagtaaatcc taaaaataag ctattggcgt gacgtataga
    26341 atatgataga ttaaatggta gcgtaaacct agtaaaggag ccgatcgcta agtgatgaaa
    26401 aaatgtccaa attgcggatc gaaaaaaatg gagcagaata ctatctgtcc agactgcggt
    26461 catcaaataa ctgcagaaga tcagcaaata aaagaatcaa ttgaagaaac aaatacagat
    26521 gcagctatgc aggataaatc aactgaagat gcagtctttc ctgatacaga attaaacgat
    26581 ccaatcgaat ggtcggaatt gaaagattta cctctagaat ctgtaatgga attattcgaa
    26641 aaagcagaac cagaagacga agaaccacct gctcacgatc aagtaactaa tatagagaaa
    26701 aatgaacaga agaaagcaac tgttcatgag acttcggaag aatcaggaaa gaatcaagaa
    26761 gaaacgcttg aatcaaaaca aaaaagaaga gtagctgaac tgaaagaaat cgtcgataat
    26821 gaggaagaaa attcgatact atccgcttat atcaaagcac atcgtgaaga tacaaaagaa
    26881 gagcacgcga aagagctgct caaaatgatc agtgaaaaaa ttgcacagga aaacaaagta
    26941 gagccagaaa aggaacaaga aactgaaaaa gactctgata gagaaaacgc cacaggtaaa
    27001 gaaacagtaa aaataaaaga cgtaaaaacg gatatcacag aaaaaactga agtatttact
    27061 gaaaaacaat tagaaaagga aaaaacgaat aaactgccgg aagcagagaa aaaagaaata
    27121 ctcgaaaaca cagacaaaaa gtcaacagag cccgatgaaa taaatagtga atccgatgct
    27181 gaaaatcaat ctgtaagcaa agagggtact gatcttgaag caaaaataga aaaagaagag
    27241 aaccagccta agctgtttga agcagaagcg aaatcaataa caatcgtgcc agatactgaa
    27301 aaaacacatc aagaatcaaa cttagaaaaa acagatccag ataaagcgca aaagaaaaaa
    27361 tcaaaaaaag taccttatct cgttttagca gcagtacttc tactcggtgg aggaagctgg
    27421 gcttattatg accatcatca aaaagtagaa gccgaagtag cagcagaagc aaaaagacag
    27481 caacaaatgg aagaacttca tgagaaactt gctgcctttt atacagatga tcaccatcaa
    27541 tttattcgtg cagatatgat tcatcaagat ttatcaaaac taaaaaatga tttatccagt
    27601 cttaagaatg aaaaaggata ttcagagtta gaaacaactt accaagatat ccaatctaag
    27661 atccgagata tccaaaaagt gaatgagtgg ttcatagcac ctgccatcgt cgatgatcat
    27721 ctagcagatg acatcaagtt gaaagcagat aaagagatcc aagcaatcga aacggataac
    27781 acagattttg gaaaattgat tcaacaagca caaacagaag ctaaaaatca atatgctgaa
    27841 ttgcaaacag ctaaagaaaa aacagctata gtctttaaag acggaaaagt agtggattca
    27901 gcaacacgcg accaatacaa gacggcaaaa gaagcagtag aaaaagtgaa aaatacagat
    27961 ctatctgctc agttgaatga acaattgaaa caagtagata agatgctcac tgataaagag
    28021 aaaaaagaag aagaggaaaa gaaaaaagca gaagaagcga aaaaagcagc tgaagaacaa
    28081 gcaaaacaag ccgctcaagc agcagctgca gctcaagccg ctcaacaagc tgcggtcgca
    28141 caagcagcac agcaagaagc ggcatcctcg accccttcct cttcaaactc agcgaatcag
    28201 ccaatcaaat ctgccagagc aagtgatatt gccgatgcat ctaatccagc atggacgtgg
    28261 gcaccaggtg tccaaagcaa agtgattgct acatgtatcc aacgtggata tatcgttgaa
    28321 ggaggttaca gattagaaaa agccagaatc gaaaatgggg aaggctatta taatctatat
    28381 gccacttcaa caaaatctgc attgatgaaa ggcattggag aaagtgcttt gccattttac
    28441 atcgtaacca tcaactgtaa aactggatgg ttcggaggaa atgggagcaa ttaattttag
    28501 gcaaatgaaa gaggctgtga gcctgccgtt taacaccgtt tattcgaaaa aggggagtat
    28561 gacacaactg ttgtcatact ccttttttca cctttccaaa ccaaggctca gctattcaag
    28621 tagataacat tatgttatga tagcaaagaa atgtattaag aaaacctttc actatagagg
    28681 gagaaaaaaa tgaacgattt attagcaact atatttacag gtattattat tgatgaaaat
    28741 gaaaaaaact actttgtcca aaaaaacgga caaacctttg gcttaagcaa atcagaaggt
    28801 gaacacaaaa tcggtgaagc agttgaagga ttcggttacc aaaatcaaaa acatgaaaat
    28861 cggatcacta cacaaattcc taaaagccgt atcggtcatt acggttttgg aatcgttact
    28921 gctacacgaa aagatttagg tgcgtttgtc gatatcggtc ttccagataa agactttgtc
    28981 gtatctcttg atgaattgcc gacaatgaca gagctatggc ctaaaaaagg ggatcgcttg
    29041 atgatttccc ttagagttga tgcaaaagac cgtatttggg gcagtttagc agatgaaaaa
    29101 atattcaaat ctttaagcaa atatggaaca gaagaaatga aaaacaaaga tgtgatagga
    29161 acagcatacc gcttgaaatt aacaggtacg tacgttttaa cggaagattt ctatcttggg
    29221 tttatccatc cttctgaacg atataatgaa cctcgtctag gcgagcagat aaaaggtcga
    29281 gtgattggtg tccgaccaga cggtgtactg aatctttcat tgaagccaag agcatatgaa
    29341 gctatatcag atgatgcaca aatgatttta accattttag aacgttctgc agatcagcaa
    29401 atcaacttta cagataaatc ggatccagaa gaaatcatga aagcttttgg tatcagcaaa
    29461 ggagcattta agcgcgcatt aggtaatctt ttgaaacaag gattgattac acaagaaaac
    29521 ggtgtgacaa aactagtcaa aaaatctaat tgagaatccc ttgcatgttt ttcttaacta
    29581 gcttataata attttaatta acatagtgtt atatcttttt ttatttgtaa ttattataaa
    29641 ttgttggggt tgagtaaatg gtcatgaatg ctatttcggc gatgaaaaaa acaaaacagc
    29701 agctacatga agcggggttt aagctgacac cgcaaagaga agctactctg cttgtgcttt
    29761 tggaaaatga gaaagagcat ttatctgcag aagaaatctt ctttttagtg aaaaaaaaga
    29821 gtccggaaat tggacttgct actgtttatc gaacccttga aatcttgaca gaattgaaaa
    29881 ttgtcgataa agtaagtttt aatgatggat tagcccgtta tgatttgaga aaagaaggcg
    29941 ccaaacattt ccatcatcat ttactatgct tgaactgcgg cacgatcgag gaagtagaag
    30001 aggatctttt acttgaagtg gagcaagtag tagaagaacg ttatcatttt ttagtaaaag
    30061 atcatcggct aacgttccat ggaatctgtc aggaatgcta tgaaaaagaa aaaagaaacc
    30121 agtccgtctg agtgtctaga cggctttttc tttgctcaga ttatggaaaa agactatcag
    30181 aatgaagcgg tatctgatat gatagagagg catgaggggg attattatga aagagcaaat
    30241 caacgattat ctccactact tgaatattga acggggattg tccgccaata cacgacaaag
    30301 ctatgaacgt gatttggaac agtacttgca ttttctacag gagcagaaaa ttgcttcatg
    30361 gcaggatgtc gatcgctttt tagtcatccg ttttctggag aagttgcatg aagaaaaaaa
    30421 agcgacagca actgttactc ggatgatctc aagtctcaga agattccatc agtttttacg
    30481 gcaagagcgt atcactgatc atgatccgat gcaacatatc gatacgccga agaaagcaca
    30541 gaaattacca agtacattga ctctgacaga agtagaaaag ctgattgaag cacctgatac
    30601 gacaaaaacg ttagggataa gagatcgtgc aatcttggaa gtcatgtatg ctactgggat
    30661 gcgcgtcagt gaattgattg gattgaaatt gggagatctt catctttctc ttggactggt
    30721 acagacagtt ggaaaaggag ataaagagcg gatcattcct ttaggtgatt atgcgatcca
    30781 atggctggaa cgctacttag acgaagcacg tccattactt gtaaaggacg catctgaaat
    30841 gcatgtattt gtcaataatc atgggaaagg tctttctcgg caaggaatct ggaaaaatct
    30901 gaagcagtta gtacgagagg ctgggattta caaagaagta acaccacata cgctgcggca
    30961 tagctttgcc acacatctgc tagaaaatgg agctgatttg cggacagtac aggaattgtt
    31021 aggtcacgcg gatatctcta cgactcagat ttatacacat atcacgaaga aaagaatgac
    31081 ggatgtttat aagcagcatt ttccaagggc ttagtcatag gaaggtgaga agatgttaac
    31141 acattaccgg aaagaaaacc gtaaaattgc tatgggacta ttgagtttcc ataagaaact
    31201 gacaggtaaa acttctttgt tgaaggagat tgatacgtac gaatctgaag acgattatga
    31261 actttttttc tttacgccag aaggttcaac caacattcaa gggattattg gtatttactg
    31321 ggcaacgaac gatcagctga tcatccatga catttctttg aatccgtctt atcgcggtga
    31381 gaatatgggc tttcaaatgt tagatgaatt acaggaaaag tacccggatg tctccatccg
    31441 tgcaactgaa atcacagagc cttacattgg aaaatggcta gcaaagaaaa agaagaaaga
    31501 gtgaatgagt tgacggagat caatattaaa ttagatgtat tcgagggtcc tctcgatctg
    31561 ctgcttcatc tgattcagca aatggaaatc gatatatatg atataccgat cgctacgatc
    31621 acggagcaat atatgaatta tatccataca atgaaaacac tcgagctagc tgttgcagga
    31681 gaatacttag tgatggctgc tacattgatg gcaatcaaaa gcaagacatt gcttcctgtc
    31741 caagaattcg tcacagacga tgaagaatta tgggaagaag atcctcgaga agaattggtc
    31801 actcagcttt tagaataccg taaatataaa tatgcagctg aaagactgac agaaaaagca
    31861 gaggaacgaa gtctttatta cacaaaagaa cctatgaaca tcgatgggtt agcggatgct
    31921 gaaaagccgt tgaagcgagg acaagtcaaa aaaatcgatt tgatccgtgc catgcagaaa
    31981 atcttagaac gtaagaaatt ttctcaaaaa gtggaaaaaa cgatcacgcc cgatgacaca
    32041 acgatcgaag aacgaatggt atttatcgaa gagcgtataa aaagaaatgc gcatggctgt
    32101 actttcgatt ctttttttga gtcaccttct aaaagtgaag ttgtcactac ttttatggct
    32161 ttgctagaat tgatgaaaaa agggcagatc attgcgcaac aaaaagaaaa ctatgaatca
    32221 attatgcttt atccagcagt aggagatacc gaacatgacg aaattaagtg aacttgaagc
    32281 ccttcttttt attgttggcg aggaagggat cagtaatgaa gaattaagcg gactgttaaa
    32341 tctcagcgaa gcagaagtca caagtttagt gaaagaatta aaagaatctt acactcaaaa
    32401 ctcgcactca gctttgcata tttttgaaac ggaaagccat tatgtattaa cgactaaaaa
    32461 agagctggct cccttactta agcgatatgc gcaagggaca tcaaacacat tatctcaagc
    32521 cgccgtcgaa accttagcaa tcatcgctta taaacagcca atcacaagag ctgaaatcga
    32581 gcagattcga ggagttcagg tttcaggagc ggtacagcgt ctagctgccc ataaactgat
    32641 tgaagaaaaa ggtagagtag aaggaccagg tcggccgata ttatatggaa caactggcta
    32701 cttcttagat tattttggtt tgaaatcatt agaagaactg cctgatacac aagaaatgga
    32761 aatttcactt gaagaagacg tgtcaccaga tctattcttt gatggaatga ataaaggaga
    32821 ataattgtaa tggagagatt gcagaaagta attgcccacg ctggcatcac ttctcgtcga
    32881 aaagcagaag aattcatcct tgcaggacgt gtaaaagtaa acggacaagt tgtaagggaa
    32941 ttagggataa aagttgggaa acatgacact gtggaagtgg atgaagtccc aatttatcaa
    33001 gaagaatacg gttactattt attgtacaaa cctaaaggag ttatttctgc tgttgcagat
    33061 gacaaaggaa gaaaagttgt tacggatctt ttgccgatgg taaaagaacg tatctatcct
    33121 gtaggtcgac tggattacga tacttcgggg atcttgctac tgacaaatga tggagatttt
    33181 gctcagcgcc tgacacatcc aaaacatgaa gtggataaag tttacgttgc aaaggtaaaa
    33241 gggatcgcta caaaatcgat gcttgcacct ttgacaaaag gaataaagat cgaaggacga
    33301 aaaacatctc cagctaaatt ccaaatttta tcagtggatc ctaagacaaa ccacagtatc
    33361 gtggaattga cgatccacga aggacgtaat catcaagtga aaaatatgct tcaagcagtt
    33421 ggtcttcctg tgcaaaaact aaaacgtgaa aaatacggag atctgacttt acaaggccta
    33481 cgtcctgggg attaccgtga actgaacaaa aaagaagtca gcagtttgct gaaccactca
    33541 aaatgaacac ttacttttta taagtgttag atgttgcgtt ttttgaatag atatgtataa
    33601 tgaatatgta aacaaaaatt atataaggtt cgtcttcagg ggcagggtga aattcccgac
    33661 cggtggttat agtccacgac ctattttgca aaaaaatagc tgaatcggtg aaattccgat
    33721 accgacagta tagtctggat aaagaagata gagcttattt gacgacgctt gatcaaataa
    33781 ctctaactct attttccctg gaaaatagag ttttttcatt tggagagaaa cgtcaaaaag
    33841 ttcgctgaag aggagtccta acaggaggat ttcaaatgaa gaacacccgt gtacagaaaa
    33901 tggttgcagt agcgatgttt gctgcgatcg cattagtttt acagtatttt gcttttccga
    33961 tcatgccggc ttttagtttt ttgaagatcg attttagtga tatctcggta ttgatcagca
    34021 tgttcttatt cggtccagcg gccggagttg caacagcgtt cttgcgttca gtattacatc
    34081 tgctgactac aggtttttct ccggataatt tggtaggaga cactgctagt ttcttatcaa
    34141 caacgatctt tacattcccg atttattatt tctttagtgt aaagaaacag cataaaggaa
    34201 gaaacaaatt tttaggtgtc attacaggaa ccatcgctct aactattttt atgagtatcg
    34261 caaattattt cgttattacg cctctttact taatgttttt agggctgaac gcaacacaga
    34321 tgctgggcat gtctttagca aactatgtac tgattgggat cgttcctttc aacttgatca
    34381 aaggattcat cgtgagtgct gtctttttag tgctgcatgc gcaacttctt ccatggttaa
    34441 gtcgaaaaca acatcagttc gaacaacgtc atacagcagt caaataaata tcaaaaagat
    34501 caagaaattt cttggtcttt ttttttggaa aaaaatacaa gacatgcaat aatgtaaacg
    34561 ttttatttgt ttgttttgag tatttttttg atttatgcat aaaaatcaat caattctttg
    34621 caaatgtgaa attcttctcc ttttaaaaga cgaaaatgtt tgtttataaa aaaaaggaaa
    34681 cgctacaatg aaaaagaaga aagctttaaa aacctgaaaa aagaggaggg aatagggaaa
    34741 aacgaacttt ttgaacaaaa gttttacaaa atgcgtgtta ttatttttgt tctgatactc
    34801 tttttgataa aataacatta gagccaaaat tgttgtctct aacggaaaag aaaggaagaa
    34861 gaagatggaa caaacaaaaa agaaaaaatt tcaaatgcct tcagcgtata cggtcttatt
    34921 tattattatt gcagtaatcg ctgtacttac ttggtttata ccagctgggc actatagtgt
    34981 agacgatgca ggcaacatcg tcgctggatc atatgaacga gtacaacaaa atcctcaagg
    35041 gctgtgggat attttcatgg cgccaatcaa tgggatgcta ggtgtaccag cgggggagct
    35101 aactaaagaa acacctgctg caatcccaat ctctttcttc attttggttg ttggtggatt
    35161 tttaggtatt atcaacaaaa caggggcact agatgccggg attgcttcag ttgtgaagaa
    35221 attcaaagga aaagaaaaaa tgcttattcc agtattgatg ctgttatttg cattaggcgg
    35281 ctctacttac ggaatggcag aagaaacgat tccattctat gctttactga tcccagtcat
    35341 gatggctgta ggctttgata cagtagtagc tgtagcaatc gtattagtag ggtctcaagt
    35401 aggctgtcta gcttcaacgg taaatccttt tgcaacaggc gtagcttcac aagctgttgg
    35461 gatttcgatg ggtgaaggta ttggtcttcg attcttgatg ttcgttattt tgttagctat
    35521 ttctattgta tatgtgtatc gttatgcatc aaagatcgaa aaagatccaa caaaatcatt
    35581 agtatacaac caacgtaaag aagatatgaa gcatttcgat attgaagcaa tcggtcgtca
    35641 agaagtaatc acgaaaaaac aaaaacatgt taatgtattg ttctttatta cttttggtat
    35701 catgatcatc agtttgattc cttggacaac cttgaatgcg aatttcacga tttttgaatc
    35761 attgacaaac tggttaacaa accttccggt actaggtacg gttctaggta aaagcatgct
    35821 tccattaggt gactggtact tcccagaaat tactatgtta ttcttagtaa tgtccatcgt
    35881 agtagcaatc acttatggca tgaaagagtc tgaatttgtc tctgaatttt tagctggtgc
    35941 ttctgatttg atcggtgtag caatcgtagt agctgttgct cgtggtatcc aagtggttat
    36001 gaacaatgga ttaatcacag acaccatctt acattggggc gaacaaggat taagttcatt
    36061 atcttcaagt gtattcatca ttattacatt tattttctat atccctatgt cattcttgat
    36121 tccttcaact tccggcttag ctgctgcaac aatgggaatc atgggaccta tgggtgaatt
    36181 tgccggcgtc ggtaaggatt tagtaatcac tgcttatcaa tctgcatctg gtttagtaaa
    36241 cttaattaca ccaacatctg ctatcgtaat gggtgctgta gcaattgctc gtttcgatgt
    36301 ttcagtgtgg tggaaattta ccggtaaatt aattgcgata ttatttgtag ccatctgtgt
    36361 gatcctaggt gtagcagcaa tgttctaaac ctagacaaat acaggtacaa ggtctatcat
    36421 aagaatgaag aaaccgataa acggctatcg ataagcacgt ggataagtca atagctccat
    36481 tcagtgagtg ttttttcttt catcatttgt taagaacatt ctgcttgctg atggagcagg
    36541 cttaaaacgt cattatttga gaagaagagg ttgtgtcgga ggaatttgac atgacccttt
    36601 ttaacatatt aggaggataa tatcatgaaa aaatttgtga cagaacaaca tcatgaacaa
    36661 gcattagaat cattgagcga attgatccga attccttccg tactagatga agcagattca
    36721 ggacaaggac atcccttcgg taaaaaagtg attgaagctt tggataaagt attggaaatc
    36781 agtgaaaagc taggatttag aacatttaaa gatccagaag gctattacgg ttattcagag
    36841 attggctctg gagatgaatt attcggtatc ttgtgtcata tggacgttgt tccagcaggg
    36901 gacgaaaata attgggaaac aaaaccattc gatccaacta taaaagacgg ctggttagtt
    36961 ggtcgcggat cccaagatga caaaggtcct tctattgcgg caatgtatgc cgtcaaagct
    37021 ttgattgatg ctggcgtgga attcaagaca cgtattcgtt ttatctttgg tacagatgaa
    37081 gaaaatttat ggcgttgttt agaaaaatat aacgaaaaag aagaaggaat tacgcaagga
    37141 tttgcgccag atgctgaatt tcctttaatt tatgcagaaa aaggcttatt gcaagcttac
    37201 ttgacaggac caggaacgaa cgagttttct gtaaaagctg gaggagcgtt gaatgttgtt
    37261 cctgatgcag ctccttactc tggagaaaag ctttctgaag taaaagaagc attgaaaaaa
    37321 cacgaattcg atttcgaaga acaaggtgaa ggcatcgtcg ttcttggtaa aagtatccac
    37381 gcgaaagatg cagcacaagg agtgaatgct atttctcgtc tggcaattgc tctttcagaa
    37441 gtatttgatt ttggcccaat caatttctta ggaaaattag ttcaagaaaa tgcaactggt
    37501 gaagctgtcg tgggcaaaac agaagatgaa caatccggcg agctaacgat gaactttgca
    37561 agtttggaaa tcacaccaga acaaacaaaa atcggtgtag atatgagaat ccctgttaca
    37621 ttcaagaaag atgatttagt agctaaatta actaaaacag cagaaaaata tgggttgact
    37681 tatgaagaat ttgacttcct tgattcatta tacgtgccac tagacagtga gttagtgaaa
    37741 aatctattgg ggacatatcg cgatattact ggagacatga ctgaaccgtt tgtatcaggc
    37801 ggcgctactt ttgctcgtac gatgaaccag tgtgtggcat tcggtgcgat gttccctgat
    37861 actcctgact ttatgcacca ggcgaatgaa cgatgggaat taagtagtat gtataaagca
    37921 atggaaattt atgcagaagc tgtgtatcgt ttatgtgcta aataaaaata aacatacaaa
    37981 aagggcggat attcaaatgg atattccgtc tttttttatt tgatgctgga aagtttgcat
    38041 ttctctctta tttttttaag gtataatcat acttgattaa gaaaaagggg tcactcagat
    38101 agaaaaggtg ggagaaagtg aaaatattta gacgaatact aattggctta gtcatcattt
    38161 taggattact cggtggagcc ggttattact atatacagca aaatacgtac gaaccatcgt
    38221 cagaagcgca agtgttggta aaagaaggaa aaaacaaaga agattactta ttttttgaag
    38281 gagagaaaaa gaatcatgga aagtctccat tacttatttt ttatcctggt gctctcgtag
    38341 agccagaaag ttatagtatt tgggcgcaag atttagcaaa aaacggcttt tcagtcgcta
    38401 ttgtcaaaat gccacttgat ctagcagtga tgggcggaaa caaagcggat aaggtcaaag
    38461 aagattttcc aaattccgat tatgtgatcg gtggccattc attaggtggc gtaatggcaa
    38521 gtcgttatgt caataagaat cattcagaca aacatttaaa aggggtattc ttcatggcaa
    38581 gttatcccga caaaaaagga acattgacag aatcagctgt tcctgtttta tcgatcacgg
    38641 cgacaaaaga taaagtattg aatcaggaag cttatcaaga agctaagcag tatttaccgg
    38701 aaagaacaga gtatgtatca atcaatggcg gaaatcatgg aggttttggc agctatggtg
    38761 agcaaaaagg agatggagag gcagaaattt ctaacgcaga ccagcaaact gtgatcgtca
    38821 atacattgga aaactggctg aaaaagttga aataatataa atgggtccta tagggtccgt
    38881 gaaaaaacta gtcaattaac tatagatagc aaatgaatct ttcaaaataa gtcgggattt
    38941 cacggaacaa gagaaacaga tcccggctta ttttcaagtt tagagaactt agtgtacgtt
    39001 tttaaagttt cgtttatatt tatattttat tttctaataa aatcgctcga actggacatt
    39061 tttgatacgc tttttttaca tatgtttctt cttccggcat cacgaactgt tgatgagcct
    39121 ctggttcttg ggcaaataaa acgattcctt cttcatcata atcgaaaata tctggcgctt
    39181 cgatttgaca aagaccgcat gcaatacagc gttcaggtac aagtttacat agtagtgaca
    39241 tttctaacca tcctaactta aggagaataa tatgattgtt tttattttat cgctattttc
    39301 atcaggacac aagctgcgga ttagttctct ttaccaactc cttgtgggaa aacgaacgac
    39361 ttcagtactt atttatggat ttacccacga acttttgttt atccataaca gttttccaga
    39421 attgaagcag gataagtttt atcaaatttt gcaaaaaatt gcgcagcaag gttggatcga
    39481 aataaatgaa aatgaagcaa agctaacgcc tgcaggggct gatatgttgt ccgaacatca
    39541 aatagaacat actggtttgc gttttgaccg ctatggcaga acaggggaaa ctagttggcg
    39601 gctcattaaa tttgctgttc aagtaatttc taatctggca acgggtattc aagactatct
    39661 tccagcagag acaagtcctt tttatacttt ccaactaaaa aaatggctta gtgaaagcca
    39721 attgccacgt gaaattttga ttgatactgc gtatgagagt ttggtgcagc tgttttcgga
    39781 gataccagaa gaagcagcag attttttagc taaccaatta tctggaaatg agcgaacagg
    39841 gctgcttcct tatcagctgg caaaaaacaa tgatgagtca gcggtttatc tgcaacaaag
    39901 cagatgtatc caccttttgt tagcacaaat cgaaaaacgg ccagattcgc tttggcacgc
    39961 tttgatcgat tcattattac agcaaaattt caatcaaagc atgatgatca ccaagcagat
    40021 gtttatgaat ggtcagacaa ttgaccagat catggcaatc cgtcatttaa aaagaggaac
    40081 agtaacagat catcttattg agtgggcctt gttttttgat gattttccat acgagtggat
    40141 tctctctgca gaaacaatcg aacgtttgga accaaataag gattccgtgc gagagtggcg
    40201 ctttagcgaa tggaatgtag atggacaatt agattatgga gaatttcgtt tgtatcaaat
    40261 ctatctattg agaaaggaag cgatacagaa tgttaataaa tgaattaaaa aaatattttg
    40321 gatttgattc atttcgtccc ggccaagaag aaattattca ggcgttgctt gatggagaag
    40381 atactctcgc tattttgcct acaggaacgg gaaaaagcct ttgctatcaa ttgacggggt
    40441 atttaatgga aggtctggtc atcatcgtgt caccgctgct ttctctaatg gaagatcaag
    40501 tgacgcaatt acaaaaacga ggggagaaac gagtagctgc tttcaatagt ttactttcgc
    40561 aatctgaaag aagatacgtc ctccgtcatc tatcgcaata taaatttttg tttttgagtc
    40621 ccgaaatgtt gactaatcct tctcttttag agcatctgaa aaaacaaaat attgcgttat
    40681 atgtagtaga tgaggctcat tgcgtgtctc aatggggagt tgatttccgt ccagaatatc
    40741 aacagttagg aaaaatccga aagcttttag gaaacccggt cacactggca ctaacggcga
    40801 cagcaacaga tttagtagca agagatatac gtcatgtact atttgatcga ccaccaaaag
    40861 agatcagaca atcagtcaat cggcaaaata tctcgctttt tgttcggaaa acgcagcaaa
    40921 aagaacaaga acttgaacac ttcatggaaa gagcacaagg agcggcaatc atttattgtg
    40981 caacaaaaaa agaagtcgaa cgattgtatc atttattcag ggaacgtttt acagttggat
    41041 actatcacgg tggtcttgat gcagctcaaa gaagacaact tcaacagcag tttgtaaaaa
    41101 atcagctgca gtttctcatt gctacgaatg catttggtat ggggatcgat aaatctgata
    41161 tccgttatgt ggtccattat gatttgccag acagtctaga aaactatgta caagaaatcg
    41221 gtcgagcagg cagagatcag gaagaaagtg cagcaattct tttttatcaa agtggggatg
    41281 aacgtatcca ttatttcttt aatcaacttt ctagagaaca gcgacaaagt tttgagcttt
    41341 atttggaata tgccacagag caagctccct ttgatgagct gcaaaaaaaa tggatggagc
    41401 tgatccagca gtctgaaaaa ccagaaaatt gggtggaacg gctcaaacgt caggaaaaag
    41461 aaaaagaatt ccgcctgcag cagatgcttc gctatattaa tgaagaaaac tgccgcagaa
    41521 aattcatctt agcttatttt ggcgaaaaac tctccgaaaa gccgcagaat tgttgtgata
    41581 ttgatggagc aaaagcagaa cttctgccaa agaggaagct tttttctaag actgaacaac
    41641 ttcattggga aacgatttta ctaaatttat ttaaaaaaaa catggaacat tgatttttta
    41701 ggacaagaca ctttttccta tggtataata cacacgatag agtttaggag gtaaaacagt
    41761 gagcagaaaa gatagacatc aatctacaga aaataacgaa acacaagaac catgggaaca
    41821 accaatctac gatacagatg atgaagtttc atcaagaagt tctcagagac aacaaaagaa
    41881 aggaaataca ttatttcttt cgctattttt gattttactg gtcttatgta tcgctattcc
    41941 agcaggtgct tatttctgga tcagaaatgg atcaagcaac aatgcaacag cagcaacttc
    42001 ttctgaaact tcgacatcag tagtagaaag ttctacaagt acaacaaaag aggagtcaac
    42061 tgagacttca acagtagaat ctagtacaat cgcggaagaa tctactgtgt ctcaagatac
    42121 aggtgtaacg tcagaaagca gtattgcagc agagacaaca ccgacttcct caagtgaggc
    42181 aacttatcaa gcaggggatt caagcagcaa tgcagcagaa acaacacctt cttcatcagc
    42241 tgcagccggt tcaacaacga ctgttcaagc aggcgaaggt ccaaaacaag tggctgagcg
    42301 tgcagggatc accactgatc aattgtttga attgaatggc ttagatccaa acaactttat
    42361 gctttaccct ggtcaagaat taagaattaa ataataatag ccgcgctttg cggctttttt
    42421 tctgagaaaa aggagttata tcatgacaca aatcagtatt gcaatcgacg ggcctgcttc
    42481 atctgggaaa agtaccgttg ccaagatatt agcaaaagaa ttgaattata tttatacaga
    42541 taccggtgcc atgtatcgcg cagtaactta tttagctatt acgaacaatg tatctttttc
    42601 agatgaagaa gggctagttt cgctgatcaa aaagtatcct atctcttttt cgcaacaaga
    42661 agatggacag catgtattca tagcatcaaa agatgtgact ttagaaatcc gtcaaccaga
    42721 tgtcacaaaa gctgtttctg aagtctctgc acatggaaaa gtcagagaag aattagttgc
    42781 tgtccaacga aaaatagggg aacgcggtgg cgttgtcatg gacggaagag acatcgggac
    42841 agctgtatta ccgaatgcag aagtgaaaat ctttttagta gcaagcgtaa gtgagcgtgc
    42901 agaaagacgt ttcaaagaga accaagaaaa aggaattccg actgatcttg aaacactgac
    42961 gaaagagata caggaaagag accattatga ttcaacaagg gacgtctctc cgctgaagca
    43021 agcagaggat gctgttcgta tcgatacgac tggcaaatcg attccagaag tcgttgcagc
    43081 aataaaagct gttgttttga aaaaaggtta tcttcttttt taaattaaga aaaaaaatga
    43141 aaaacaattg ttttttcaag gaaaacgaac gaaatcgctt tatttttttt gaataatcaa
    43201 ttaaactaaa aacagtaccg tacttacggc agaatgttgg ttaggaggac aagtgttatg
    43261 acagaatttg atcaaaatca agagaacaac cagtcaatgg aagacgcaat gaaaagcgta
    43321 caagaagtaa aagttggcga tgttgttaag ggcgaagttt tagtcattga agacaaacaa
    43381 gtgattgtcg gaatcgaagg ggctggagtt gaaggagtcg ttccggcaaa agaattatca
    43441 actttgccgg tagaagatat cagcgaagta gtaaaagtcg gtgatgtatt agatttagtc
    43501 gttatcagta cgatcggcaa agataaagaa aacggcagct atctactttc aaaacgccgt
    43561 ttagatgcta aaaaagtttg ggaagatatc gaaagagatt tccaagaggg caaggtcatc
    43621 gaagcacctg ttacaaatgt tgtcaaaggc ggtttagtcg ttgacgtcgg tgtacgtggt
    43681 tttgtccctg catcaatggt ggaagatcat tttgttgcag acttttcaga ctataaaggc
    43741 aaaacattga gctttaaaat catcgaaatc gaaccatcag aaaatcgttt gatcttatcg
    43801 cataaagcgg tagtggaaaa cgaaagagaa gaaaagaaaa aagaaattct ttcaactatc
    43861 catgagggag atgttctcag tggacgtgtt gcacgtctta ctgattttgg cgcatttgtc
    43921 gacctaggcg gaattgacgg tctagtccat gtctcagaga tttcacatag tcatgtagca
    43981 aaaccaagtg atgtattggc tgtcaatgat gatgtaaatg tcaaagtgtt gtctatcaat
    44041 cctgaacaag aacgtgtttc cttgtcaatc aaagacacac agcctggacc ttggtcagaa
    44101 atcgatacaa aagctccagt tggcacagta ttagacggaa cagttaaacg tttgacaagc
    44161 ttcggtgcct ttgttgaagt gttccctgga gtagaagggc tagtccatat ctctcaaatt
    44221 tcacataaac atattgcgac accacatgaa gtgcttcatg aaggtgacga agtgaaagtg
    44281 aaagtattag agatccatcc agaagaacat cgtattgcat tgagtatcaa agctttagaa
    44341 gaaaaaccag cttcagaaga agaaccaaaa aatgttgagt cttatgaatt accagaagaa
    44401 aatactggat ttactatggg agacatttta ggtgatgcgt tgaaagaaga agtagaagaa
    44461 aaagcaaccg atgattctga aaaataattg attttgtatc tgtgactgag atgaaagcag
    44521 ctgcttttgt ctcagttttt cttttgaaaa gcgattagta tttcaataag gaataaaaat
    44581 acttcgttta attgacaatc tatgcttgaa agttggcaat gcattcgtta aactaagtga
    44641 gattggaaac ttgtgaggag gaaataatat ggcaaatcca acaattgcaa tcgttggccg
    44701 cccgaacgtc ggtaagtcta cgatttttaa ccgcatcgct ggtgagcgta tctcgattgt
    44761 cgaagatact cctggagtaa ctcgtgatcg tatttatgca aaaggtgaat ggttaggtcg
    44821 agaattcagc gtgatcgaca caggcggaat tgatcttggc gatgaacctt ttatggatca
    44881 gatcaagcat caagcagaaa tcgcgattga agaagcagat gtaatcattt gtgtagtcag
    44941 cggacgcgaa ggcgtgacag acgcagatga gatggttgcg aaaattttat acagaagtaa
    45001 taaaccagtg attttagcag tcaataaagt agataatcca gaaatgagaa acgatatcta
    45061 tgaattttat tccttagggc taggcgatcc ttatcctgtt tcaggaagcc atggactagg
    45121 gattggtgat attttagatg aagcagtgaa gtatttctct gaagagactg aagaagagga
    45181 cgacgacacg attaagttta gtttgattgg tcgcccaaat gttggtaaat cctctttgat
    45241 caatgcgatt ttaggagaag aacgtgtaat tgtttcggat attgaaggaa cgacccgaga
    45301 cgcaatcgac acccacttcg tttctgaaag tggtcagaaa ttcttaatga tagatacagc
    45361 tggtatgcgt aaacgcggaa aagtatacga aaatactgaa aaatacagcg tgatgcgtgc
    45421 aatgcgtgcc attgaccgtt ctgatatcgt tttgatggta ctgaatgctg aagaagggat
    45481 tcgggaacaa gacaagcgag tagcgggtta cgcccatgaa gctggtcgtg gaattattat
    45541 cgtcgtgaat aaatgggatc ttgtaaagaa agaaaccaat acaatgcgtg atttcgaaca
    45601 agagattcgt gatgaattcc ggtatcttga ttacgctcca atcgtttttg tttctgcagt
    45661 gactaaacaa cggttggaac gtctgcctga aatgatcgaa caagtaagta tgaaccagaa
    45721 tcttcgtatc tcttcggctg tgttgaatga tatcattatg gatgctgtag caatcaatcc
    45781 aacaccaaca gataaaggaa aacgcctgaa gattttttat gctacacaag tcgctgtcaa
    45841 accacctaca tttgtcgttt ttgtcaatga agaggaattg atgcattttt cttatgaacg
    45901 tttcttagaa aatcaaatca gaaaagcatt tacttttgaa gggacgtcca tccgaattat
    45961 ccctcgtagg agaaaatagg cattttacca tatcgacgtt tttttttcaa aagaaaaaga
    46021 cagtttcgtt tgttacgttt ctatgactaa aaaaagcatg tgaaaattgg taaaaacctt
    46081 gctattaagc ggttcatatg atatcgtgat aacagaatat tgaagttaat caatatttct
    46141 ttttaaactt ctggaccact cagaaagttt taactaaatg atatttctat catatctctt
    46201 gtggaggagg tgaatctatc catggcaaac aaagcagaat taatcgaaaa agttgcttca
    46261 gctactgatt taactaaaaa agatgcaact gcagcagtag atgctgtatt ttcaactatt
    46321 caagatgctt tagctaacgg cgaaaaagtt caattaatcg gttttggtaa ctttgaagtt
    46381 cgcgaacgcg cagctcgcaa aggtcgtaac ccacaaactg gtgaagaaat cgaaattcca
    46441 gctagcaaag tacctgcatt caaaccaggt aaagcattaa aagacgctgt taaataagac
    46501 agttgaaaaa gcgcttgtgg ctctagggct acaagtgctt ttctttttat ttcctagcag
    46561 aaaaggaata aataagagac agagattccg aatccgtatg tcatacatag aaaacacatg
    46621 tattttattt aatttttatt tatttgcaag ttatgaggtg agctttgctc ttatcttttc
    46681 tagaatttgc ttactaatgg taaaataaaa ataagaagag gagggatgcg tatggaactt
    46741 gattatcaag tagaagatgc gttgacgaaa gtgttttacg gagaaacgct agtcagtcag
    46801 ctaagagaac tttccattga tcagccaatc ttatttttaa cgaatcagcg ctattatgat
    46861 ttgtttgcag ataagatcaa cccgttattt gcaaatcaag caaacaatga ctggtatatt
    46921 tgtgccaaca ctcaatgcaa tcatttaaca gaattaaaga acctgcttga tttcgtcaaa
    46981 cgctatcccg aaaatcaatc catgtttatc atcggatttg gaaatgaagg gatcatggat
    47041 ctggcaggtt tttttcaaaa acatacaacc cttgattcat cactatggat gatccctgta
    47101 tcgatccgtt ctctaagccg agcattaaca ccgcagcgga cgattttgca gctaccaaat
    47161 cgtgtagtgc tgcaaaccat caatctgcca gaacgcatcg tttatgatca aacaatcagc
    47221 gagcggcagg tggacggaaa gcagattgac tttttgactt ttatctgttg cggaatgatt
    47281 tgtgatcatc gttttttgca aaatctgtat aaaaactatc caaataaaaa gcaattaacg
    47341 actcggccgt ttgccgggat gatcgaagaa atgatctatt tttatcaatt agaggcagaa
    47401 aacctgtcaa attatggtaa actgtttgaa caagcttttt atctgaccga aaatggccat
    47461 ctactttctg ctagtatgaa aaaattttta ggaatgatgt ttcatttcgt gtggaatctc
    47521 caagcatatc cttttgattt ccaactaaaa aacttttttg tgtggctgga cttgcttggc
    47581 tatccaatcc actggctgga acagatttca aagatggagt atttggaaaa agttcttttt
    47641 ttggcagaaa aaagcaaaaa aatacttgtt ttagaaaaaa taggtataat agacggatac
    47701 caacatccaa acgaaaaaga attattgaga acaatgacgg tatttcaaca aatcgtcagt
    47761 gagataagag ggaattgaaa tgaatacaaa tagcgaaaaa atgttgcaag ctttatcaga
    47821 tgaagacttg tcacaagcac aaatcttttt agaaaaagca ttaaaagaag acccagcaga
    47881 tatattagct gaactaggcg aagaattgct ggctattggc tttttgatcg aagcaaaaca
    47941 gatttttgaa caactgatca accaataccc agaaaatgac ggactgaaca tcccgttagc
    48001 agaaattgca atcgaagata atgagatcga cttagctttt gaatacctgg aaaaagtgga
    48061 taagcaaagt gattactatc cgcaaagctt attggcgata gctgatttgt accaagttat
    48121 cggaatccct gaagtgagtg aagcaaaatt gaaagaagca gctgggttgc ttcctgatga
    48181 accattgatc cagtttgcgc tagctgaatt gtatttttca gttgatcgtt tcaaagaggc
    48241 agcagtggtt tatgaaacat tgttggcaag cgggataaat gaaatttcag gaatctcgat
    48301 gcaggaacgt ttaggacaat ccttaagtat gcaaggagaa ttcgaagcag ctatcgtacc
    48361 tttagaagaa gcactgaaag aagaaaggac agatgatcga ctgttccagc tagcttttac
    48421 gaatcttcaa ttgaaagaaa atcagcaggc aatcaactat ctggaagaac ttcgagaagt
    48481 taatccgcat tatcaatcgt tatatctata tctaggacaa gctcttcaag aagaagaaat
    48541 gattgaagaa gcacaaacag tgttagaaga aggaatcaaa gaaaatcctt accaagtcga
    48601 actttatcac ctggcctcgg aaaatgcttt ccgtctgcat gatagacagc gagcagaaga
    48661 acttttactg aaagctttag aagtaggaga caaacaagat gaaacattac ttactttaag
    48721 taatctttac ttggatgatg aaagatatga agatgtgata caagtgatca atcagatgga
    48781 agaaactgct aatccttatg cagaatggaa cctagcccat gcttataatg aattagaaga
    48841 ctttaccgtt gcagctgtac actatgaaca ggcttatcat gaactatcac atgaaccaga
    48901 tttcttgaaa gagtatgcct ttttcttaag agaagaaggg cagctgaaaa gagcgcaaga
    48961 attattgact tattatttaa cacttgaacc aggagatatg gaggcactgt cattattaga
    49021 tgacctaaca gagaggtgag aaaaatgcta attgatgtac gggaaaagaa gaatttttta
    49081 acgtggatgg tcaatcatgt atcttttagc cgaagagaag ttttttggat cttgaattat
    49141 cttgccaatc atgaagcgat tttaagcaat gtccactttg ttgaaggggc gaattttgcg
    49201 gatcgaggac tgcagatcag tgatctatct gttgaagggg aacccatgaa attgtttttg
    49261 cagggaaaag aatttacgga cacagaccag atttttcatg aaatccgttt gaattggaaa
    49321 aagcctttat atgtggaatg tatattcgaa aatgcttggc agacaagaga atatctatct
    49381 atcttagaag acaatccatt ttcttcttgg gattcttcag tcagtgaaga agatactgaa
    49441 ttaattgaac gatattttca agaaaaagaa gaagaggcaa aactaaaaat gctttatgct
    49501 caaatcgatc aagcactaga agatggagat agggatgctt ttttgaaatt atctgatgaa
    49561 gttaaccgaa agatcctttt gcagacacaa aagaaaagta accgggtaag tgaacataac
    49621 aattagttaa aaatagtata gtactaaaaa agacaaaggt cagctggatg tggaatcttt
    49681 cctcatagcc tatctttgtc ttttttctta tagttttttc agtaatcttg tctgtggctt
    49741 caaataagta aagccttgcc cttccacttc atgcacattg atgattgaaa ggaaagcttt
    49801 ttcgtcaatc tcgtggacga tcctcttagc ttcagttaat tcactagagc taacgaccat
    49861 atagatgatt ttttttgaat taccagagaa tgcgccttct ccagaaagga aagtcacacc
    49921 tctttgcaga ccggtcatca gtagtggtgc aatctcttcg cttttgttgg agacgacaag
    49981 tagacctttt gcagaatatc cgccatctaa gatcgaatca atcacacgac tgaaaacata
    50041 cgaaacaatc aacgtataca tcatacgttt caagtcgata taggttaatg aagcaagtaa
    50101 gacgatgata tcaaatgcca atagggaacg ccccatagtg atgccgtaat tttgttctaa
    50161 aatccgagcg ataatatcac tgccgcctgt tgttccaccc atgcggtaga cgatcccgct
    50221 tcctacacct gcaactaaac ccgcaagtag agaaacgatc agtagatcat gctgtaaatt
    50281 gatttctagt ggaacccttt gccagatcca taaaaagaat gacaaagaaa gtgtacctag
    50341 aacagtatag gccaaggaac gtttaccgag gatctttcct cctagtaaaa tcaatgggat
    50401 gttgattaat aaagtcgtat aagctggatc aataccaaat aaagctctta agatcaaggt
    50461 gatccctgag actccccctt ctgctaaatg attagcgatg ttcaagtaaa caagaccaaa
    50521 tgcataaata cttgttccta taatgataaa aagaacttct tttactgttt ttcgtttgac
    50581 catatgaatt ccagctttca attatgtatt ttcacagaat ttccatttag aaattccttc
    50641 ctaaatgtag catggaaatt cgattttctc aattgctctt atctaggatt tgcgttaaag
    50701 ttttggtaag atagttttgg agggaaaaag atggataaaa agcgttcttt atacagtatg
    50761 cagcaggaag tggatgaata tatccagcaa ttcaaggtgg ggtatttctc tccattagct
    50821 cagatggctc gattaactga agaagtaggt gaattggcaa gagaagtcaa ccacacttat
    50881 ggagaaaaaa gcaaaaaggc ttctgaacca gttaactcgg tagcagaaga acttggtgat
    50941 gttttattcg ttacgatgat catggctaat tcattgaata tcgacttaac tgaagttttt
    51001 gaaaaaaaca tggaaaaatt caatcaaaga gaccataatc gctttgagcg taaggacgag
    51061 taaacaagat gaagtggtcc tggccttgaa gactctgaaa cggacttgtc aaacagtcac
    51121 catgcaactg agtctttatc tcaggacaag aaacacgcag gaaaggttgg aaaaatgaaa
    51181 ttaaaacaat tacctctgga ataccaaaag gcaattccag ttctgaaaaa attagagaat
    51241 gcaggatatg aagcttattt tgtaggtgga agtgttcgtg atatcttgct gaaccagcca
    51301 atccatgatg tggatatcgc aacaagcgca tttccagaag aaatcaaaca gctttttcca
    51361 aaaacgatag atattgggat cgaacatgga actgtgttgg tattagaaaa tgatgaacaa
    51421 tatgagatca ccacatttcg gacggaatca acttatcagg actaccgtag accagatcat
    51481 gtcgaattcg ttcgttcgtt ggcagaagat ttgaaaagaa gagattttac gatcaatgcg
    51541 tttgctttga aagaagatgg agaaatcatc gatttgttcg atggtctgca agatctagaa
    51601 gaacagatct tgcgagctgt aggcaatcct catgagcggt tccatgaaga tgccttacga
    51661 atgatgcgcg ggttgcgttt tgtcagtcaa ctagggttca aattagagga agaaaccttc
    51721 tacgccatca aagaaaacca cgagctatta ggtaaaatat ctgtagaacg tataaatgtg
    51781 gaattcatca agatgctctt gggaaatgaa cgggccgcgg gaatccatgc atttattgaa
    51841 acagagtgct acatttgctg tcccggtttg aaagaagaag gcgaagcact tttacgtttt
    51901 gctgaattac catccgtccc attgaaagaa gagagccaag catgggcatt attgatcagc
    51961 caactggatc taaaagaaaa tgagattcgt tcctttttga aatcgtggaa gtgttctaac
    52021 caaatgatta aagacgttca agcacttgtc caaggactac gacaacgaga aactggagct
    52081 ttggaaccat tcgaattgta cttcttaggc aaaaaacatg cactgcaagt agaagaattg
    52141 atgatctatt ttgacgaaac acctcagatg gatgccgtag gagaatccta tgatcgttta
    52201 cctatcaagt cattaagcga attagcagta gatggtaaaa tcttgctaga agaaactgga
    52261 aaaaaacctg gtaagtgggt tagtgaagct ttgcaattag ctgaaaaagc cgtcgtagaa
    52321 caaagaataa aaaatgataa aaaacaggtt ttggattttt tatcgaaaga aaaattgatt
    52381 taaaaaacac tagactgtat ctgctcagct ttgttggcta cttaaagtgg gcagatacgt
    52441 cattagaaga aaaaacataa gatgtgacca cttacataaa tttgtgctaa actgaggtag
    52501 tacaagaata aattacatca taaaggagtc tttttaatga gtaaagaaat gacagcgcta
    52561 aaattttatt ttagaaatgg agaaacctgg acgatcaacc gccgtcatat cggcgatctt
    52621 tggatcaaac aaatcacaac aagttttgga cgaatcaatg gcagtgaatt tgttgaaatc
    52681 catccgtgtg ctggatttaa aatcgaaatc ttccatgaag gggacgcagt tgcgacacat
    52741 gatatcaacc taggcggact ggaaatgggc atgtttaatc gcgcattgaa atacgaagat
    52801 atcgagcgaa tggaaatcct ttaccgaaat ggcacacctg atcttgtgta cttcccatat
    52861 ctagataaag gaacagaagg attagacaat caatatcaat caacaaaaat cagcgaaaaa
    52921 acaggtaact tgtatatcgt gatcaatcca gatcaacgtg tagaagatgt atacggggaa
    52981 tttttcgaat aaatcgattt ttacgcaaaa atgaggctgt gaaagagatg tgtcttatta
    53041 ctactgatag gctgtctctt ccacaccgtt tattcagaat aagggactgt gacaagattt
    53101 ttgtcgcagc cccatttttt tgttgtgata aaagaacaag aaaggcagga atgggaaagt
    53161 aaacatcctt ttgtcagaaa cttttatctt tcttaagttt tacttgatat ctgatactat
    53221 ttggaataga atgaggcgtt gaaaaattta tggaaatttc taactagcaa taaacgatga
    53281 aacaatgaaa tatttttcat ccaaaaagaa ctttgctata atgatggaag tttgtgtata
    53341 ggagatctgg aaaatgagag agagttttga acaacaaaaa aaactgttgc atgatcgcta
    53401 tggcgccctt tcgatggatg accgccgtca aatactttgt aagttgcgta aaagaaatat
    53461 attgatgtat cgtcagttgg aacgtctgaa acatgatctg cttcgtttgg agtcaaaacg
    53521 tgtacagtgt gaattagagg ggaaccaaac acaggttgaa gtagtcgaaa ccaaaatttt
    53581 aaagaaaaaa gaacaatttt taaaaatgct tacacaaaat aaaaaataaa ggggatggac
    53641 gatggaattt gctgagttag ttattgtatt tgcagtaacg atcaccttat cgaacatagc
    53701 tagccgcatt ttgccaatga ttccagctcc actgatccaa attttccttg gagtggtatt
    53761 aggtctgaca gagtggggac aatcaatcga ttttgaacct gaattgttct tagttatgat
    53821 tattgcacca ttgttattta gagaaggaga aaaagcagat atttcttcga ttctcaaaaa
    53881 tttcgggaca attttgttct tggcatttgg aggagtcatt ttgacccttg tcggggtagg
    53941 tgcgacgtta tcattcctgt tgccaagtgt cccacttgct gcctgctttg cttttggagc
    54001 agcgttaggt ccgacagatg cggtagcagt cagttctctt tctggacgag tgaatatccc
    54061 taaaaaggcg atgcatattt tggaaggaga aggtcttttg aatgatgcat ctggtgttac
    54121 tgccttccag tttgctcttg gtgcgctgat caccggatct ttttcagcag taaacgcggg
    54181 gatgagtcta gtagtttcta gtattggggg agctttgatc ggctttttgc ttgtctggtt
    54241 caaacaaaaa ataattcatt tgatcgaaaa agcatcagca caagatgtca cagggtatct
    54301 gctgatcgaa cttttgcttc cttttctagc ctatgtattg gcagaggcat ttgatgtatc
    54361 tggaatcatt gctgcagtgg cagcaggtat tctccaagct tctggtttta ggaaaatctc
    54421 tgtgtttgat gcagaattgt cgagcttgtc ccacagtaca tggacaacta ttgcttttac
    54481 actgaatgca ttagtattta tatttttagg aatcgaattg acgcaagtgt tctcgccagt
    54541 atggggagac ggtttatatc caaacggtct tttattagcg attatcgtat taatctctgt
    54601 catgcttttt gtgatcagat tcatttctat cagtcttttt tatgtattca aagatggcag
    54661 taaaaagttc aaaaaacagt tgaacgagat cttgatctta acatttggcg gtgtaaaggg
    54721 aacagtcagt ttggctacga tttttatttt gccattttca atcaataata tgatgtttta
    54781 ccagcggtct ttgcttttgt ttctgacggc gggtgtcatc ttagtgactt tggtcatagg
    54841 gatcattgtc ttgcctatgc tgacggaaac agaggaagcg aaaagcacgg atttgaatgc
    54901 tttgatgatt ttagaagagg tcgttgaagt tttgcgaaaa gaaataaagg aaattgatcg
    54961 taatacaaaa gaatttttag ccactgaagc agtgatagaa aattatcaag aacggatacg
    55021 tgatctgtac ttggaagatt taaccgatga tgaaaagcaa gaagtacaag aaatacaagc
    55081 actgattttg tcgatcgaac gggatggatt ggatgaaagt tatcgttcgg gcaagctttc
    55141 ttctaatggt tatcgttttt actcccgttt tctttctcga ttcgaacatt ctatcacaag
    55201 ccagattttg tcttttatcg gcttttggtt cattgttgtc cgtaggttaa tgcgaatcgt
    55261 tctccatccg aaaatgtttt ggcaaagaag atatgctgat cgtcagacat ttattagtga
    55321 tgaagatatc caagaaatca gagagacata tcaaaaaaat actcagttga ttattgaaag
    55381 tttagataac ttgactgata tttatgatga aacgatggtt caatttttta tccagcaacg
    55441 caaacttgaa ggaataaaaa tgaactcggg gaatttgatt tcttcgtgga tgatccagca
    55501 agatagtctg tttacgaaaa aaatgctaag aggttattat cttgaaagaa aaatcattga
    55561 tgaatacgaa gttgctgaaa aaatcacgac gttttctgcg aacagctatc gcaggaatat
    55621 caatttgtta gaatcttata cgatgaacaa accatctgat aacttttctt tccgatttgc
    55681 gtttcgagca aagtcaaaaa aaacgagtcc aataaaataa aaaggtaaaa attttcaagt
    55741 tttataaaaa acaaaacata tcagaacagg atcgaatcgg tcctgttctt tttttgagaa
    55801 cacaaaaaga acaaatacca aaaaaatttt cctgaagttc actattttta tgatacaata
    55861 tccaagataa ggagggaaca tgttgaaaga attaaaagta actgacctca aaaaaactta
    55921 tggtgaaaaa gatttgtttg accagatttc ttttttggtc cacgaaaaag accgtatcgg
    55981 gttgattggg acgaacggga cgggtaaaac aagtctctta aatattattg ccggtatcga
    56041 tagcagcgac ggtgatcggc aaacagtgtt ttatccaact gattaccgta ttggttatct
    56101 ttctcaagca gctgaatttt ccgatgaatt aactgttctc caagcagtct ttcaaggaaa
    56161 cagccccttg atccaaactg ttcgagcata tgaggaagca ttgatcgaac taggggaaaa
    56221 cggagaagac cctgatgtgc aaaaacgtta tgctaaagca gaagaacaaa tgaataaaga
    56281 agatgcttgg actacagata ccaacgcaaa gattattttg caaaaattag gaatcagtga
    56341 attagataaa aaaatcagta ctttatcagg tggtcagaaa aaacgagtta gtctggcgca
    56401 agtgttgatt gatgaacccg atttactatt actagacgaa ccaacaaacc atttagatta
    56461 tgaagcgatc gagtggttgg aaaactattt gaaacaatac cgtggcgcac tattgatggt
    56521 tacccacgat cgttattttc tagatagagt agcaaaccga atcttcgaac tttcatttgg
    56581 aaaattatac gagtataaag gaaattatga agcttacgtc ttagaaaaag cagaacgtga
    56641 tcgtgtagaa gtagaacaag aagaaaagcg caagcggctt tataaacagg aattagcttg
    56701 gatgagggct ggcgtacagg cacgtggcac caagcagcaa gcacggatca accgtttcga
    56761 agatctaaag gagaacttgt accaagtaaa ccaagaagat gatttagagc tgaacctagc
    56821 gacacaacgt ttagggaaaa aagtactgga aatcaaagat ggcagttacc gtataaatga
    56881 ccaaacactg ctagaacatt tagatttatt gattcaatct cgggaaagac taggtattac
    56941 tggtaaaaat ggtgcgggaa aatcgacatt gcttaatatt ctagctggca gaattcccct
    57001 ggatagcggt acaatgtcta tcggtgaaac ggttcatctt gcgtattata cacaagaaaa
    57061 tgaagaaatg gcaccagata aacggatgat tgcttactta caagaagctg ctgaagaagc
    57121 aaaaacagca gatggcagtc aaatcggagt agcagaatta cttgaacgat ttttatttcc
    57181 aagatttatg catgggacac tgataggcaa actttcaggc ggagaaaaac gccggttatt
    57241 tttgttgaaa ctgctgattc agcaacctaa tgttttatta ttggatgaac cgaccaatga
    57301 tttggacata gcaacattga cgattttgga agattatttt agaagttttc caggtgctgt
    57361 gatcactgtt tcccatgacc gttatttctt ggacaaggta gcagataagt tattagtatt
    57421 ccaaggaaac ggaaaacaag aactttatta cggaaacatg tccagttatt tactgaagca
    57481 aaaagagacc cagcagcctg ctgaaaaagc gaaaccaaaa atacagagca aggaacctgc
    57541 tgaaaagaag aaattatctt acatggaaca aaaagaatgg gaaacgattg aagatgagat
    57601 tgcagaactt gaagaaaaaa tctcattgct ccaagaagag atgaatcatc aaggagataa
    57661 tttcactcga ttacaagaat tgcaaaacga cgtatctgaa acagaagcac aacttgaaga
    57721 aaaaatggca cggtgggaat acttgagtga atgggtagaa gattaggagg aaaaaggatg
    57781 gaacaagcgt atcttgactt aggaagaaaa ttgttggaag aagggcatcg taaagaagac
    57841 agaacaggca caggcacatt aagcttattc ggataccaaa tgcggtttga tctgcaaaaa
    57901 gggtttcctt tgttaacaac gaaacgagta ccttttggat tgatcaaaag cgaattgctt
    57961 tggtttttaa aaggagatac gaatatccgc tatctattac aaaataaaaa ccatatttgg
    58021 gatgaatggg catttgaacg ctacatcaaa agtccagact ataatggacc agatatgact
    58081 gattttggcc atagatgttt gacagatcct gaattcaatg aagaatataa aaagcagagc
    58141 aagctttttt gcgagaagat cctaaccgat gattcctttg ctgaaacata cggcgatctt
    58201 ggacacatct acggctatca atggcgtcac tgggaaacaa aagacggtgg ctttatcgat
    58261 caaatcaaac aagtgatcga agcaatcaaa aagactcctg attctagaag attgatcgtt
    58321 tctgcatgga atccggaaga tgttcctagc atggctttgc ctccttgtca tacgatgttt
    58381 caattttatg ttcaagaggg acggctaagc tgccaattgt atcaacgcag tggggatgtg
    58441 tttctaggcg ttccgttcaa cattgcaagt tatgcgttgc tgactcacct aattgcacat
    58501 gaaacaggtt tagcggttgg cgaatttgtt cacacattag gagatgctca tctttatatc
    58561 aatcatatcg aacaaatgaa agaacaattg agccgagaaa tacgcgcatt tccgacttta
    58621 gttttgaatc aggaaaagca gtcagtattt gattttgaga tggaagatat ccaattagaa
    58681 ggatatgacc cacatccggc gatcaaagct cctatcgctg tttagaatca gaaaggatga
    58741 gaaaatgtta gctgcaattt gggcacagga tgaaaacgga ttgattggaa aagaagaccg
    58801 tcttccttgg cgtttgccaa atgatctgaa attcttcaaa caaatgacag aagcaaatac
    58861 actagtgatg ggacgaaaaa cattcgaagg gatgggtagc cgtccgttgc cgaatcgtca
    58921 gacgattgtt ttgacgagag attcttccta taaagcagaa ggcgtgcatg tcatgcatag
    58981 tgtggaagac gtgcttgctc tggaaaaaga aacagacggt atctttttca tcgctggagg
    59041 gtcagctgtt tatcaagaat ttctgccatt ttgtacgata ttataccgta cagtcatcca
    59101 tcatgtattc gatggagatg cttattttcc acctgttgac tggtctgatt ggtcactgat
    59161 caatactagt caaggagaaa tggatgaaaa aaatcgttat ccgcatcaat ttgaaacgta
    59221 tcagcgtaat gagaatcctg taaaaagcgg gacaggcgaa ttgacgatat aaaaaagcta
    59281 tggatagact agctgttatt tagagctgtc ccatccatag ctttattttt tttattaagt
    59341 aaagaataaa acagagaagt acataaaacc tgcacctaac ataacaaaca aatgccaaac
    59401 aacatgcata tagcggatag aattcaagct ataaaagaat gcgcctatcg tgtaagaaag
    59461 cccgcctgct accatcaaaa taacaccagt cattccaaga gagtgataaa gaggaacagc
    59521 agccacgaca cagagccacc ccataaaaat atagatgatc gtagatactt ttgatactct
    59581 ctcccgttta tgcaacgtca acgctttata tataatgcca agaatcgcaa acaaccagat
    59641 taatacaaat aaagtccaac ctagccatcc tcggatactc agcaaacaaa aaggagtata
    59701 acttcctgca atcaaaagaa aaatcgaact atgatcgaat acttggaaca ccttttttgc
    59761 ttttgtaaaa atcaatgaat gaaacaaagt agacgttaaa aataaaagaa tcatcatcga
    59821 tccatagata gcataagaga caatatggag ggaggaatgc aagtgggcac cttttactaa
    59881 aagaaccact agtcccgcga tactcaatcc agtcccaatt ccatgggtga ctgcattaaa
    59941 cacttcattt aatatttcat acgttcgatt aaatttcgtt ttttccatga aaacggctcc
    60001 tttactaggt gtaattaatg aaaaatcatt atttgtctgt tatactaaaa atagaaattg
    60061 tcttacatgt agtttaacat gaataaggca tcttgttaaa gaaagagaga gagaaaataa
    60121 tgacaaatgt aaaaatcgta acagattctt catgtacgat ggaaaaaaat gtacgcgatg
    60181 ctttaggcat tcgtgtcatg ccgctatcca tcatgatcga cggaacagta tatcccgatg
    60241 atgaccaact agacggcgaa agatttatgg aaatgatggc gaaagcaaaa aatctgccca
    60301 aaacaagcca accaccaatt ggagaattca tctcgatgta tgatgagcta ggagaagacg
    60361 gcagtgaagt gatttcgatc catatgacaa aaggcttgag tggaacagta gaagcagcaa
    60421 gacaagccag taatctaaca aaaacgaaag taacggttat cgacagtgat accactgacc
    60481 aaggattatc ttttcaggta attcgagcag ctgaattagc aaaagaaggt aaaacagtag
    60541 agaaaatcct tgaagaagtc aaaaaagtac aagacaatac aaaattatac atcggaatat
    60601 ccacactaga taacttggta aaaggtggaa gaatcagccg aacaactggt ttgctgtcta
    60661 gtgttttgaa catacgcgtg gtgatgaatt tcgcccattc tgaattaatc cctgtaacta
    60721 aggggcgagg aaagaaaacg ttcgataaat ggtttaataa tttgaaaaat gaactgaaag
    60781 aattgccaaa tgtgcgacaa atcggtattt cacatgcgga tgcaaaagaa ttggcgcaag
    60841 aattcaaaga gggtttgcaa gaattattcc cggacatgca tatccctgta ctgcatacga
    60901 atccggttat cgctacgcat actggtaaag acgcgtttgc aatcatgtat tatgtagatt
    60961 agtcgaaaag aggataagcc ctcagcctga aaggcttgca gcttattctc tttttcttta
    61021 taaaattagg aaaataattg ttttcttttt tcataaagac tacctttttg ataaaatttt
    61081 tgataacatt agaaccaatc gaatacaaga ggtgaaaaaa tgaaattaac gaaacgacat
    61141 ttgatcctcg ttttgttccc attattaatt gcgatcatca tctatgcagg tttagctgct
    61201 tctataccga aagctggccc actaatgaaa ccagtaacta ctgtgagaga agatccaaat
    61261 aaaaaagaaa aacttcacta catagctatc ggagattcgc tgactgaagg agtaggagat
    61321 caaaccaagc aaggaggatt cgttcccctt gtcgcaaatg atctaaaaga ccgttataat
    61381 ttgaccgcgg ttgaaacaga aaactatggt gtaaatggtg aacgaagtga ccagatctta
    61441 aagcgagtaa aaacaaaaga agaaattcga aacaatatca aaacagctga ttttatcaca
    61501 ctgactgttg gcggtaatga tttgatgaaa gttatccaaa ataatttatt cgggttaacg
    61561 gtcaattcct ttaaaaagcc actgaaaaac tatcaggaaa atgtcacaga actattggaa
    61621 gaatttcgat ccctcaataa aaccgcacct atttacattt taggcatcta caatccattt
    61681 tatttgaact ttcctgagat cactgacatg cagacaattg ttaacaattg gaacaaagga
    61741 acagaaacgg tgacaaaaaa aatgccgaac tgctacttta tcccaatcaa tgatttgctt
    61801 tatcaaggat tgaatcagga agtaggtgtc acagaagaag gaacagatca aacaactagc
    61861 acgacagaaa atctaaatgg catcaaaaat aatgtgctct atgaagaaga tcatttccat
    61921 ccaaataatt taggttatca attgatggca aatgcagtga gagatcagct gatcgagaca
    61981 caaaaccaat ggttagagaa ggaggatgat caataaacat gaaaacaaga tctgacgtat
    62041 cttcaaacaa aacaaagaaa acaaattact ggaaattcgc cttccttatc cttcttgggg
    62101 tagtacttgg ttcgaccgta tttttaggca gccgtatttt tgctaatcgg gaaccagaac
    62161 tcccagaaat accagctctt acggaaagac aaggagatcc tgtcttaacg atcaattcaa
    62221 ataaagaaaa agtcaatcaa atcatcagct ttttccttag tgagtatcaa aaagatagtg
    62281 atattgaata taaattttat ttggaaaatg aagcactact caatggtact ttcgaagtac
    62341 taggttttcc tatcgatttc tatttatatt ttgatccgta tgtgatggaa aacggaaatg
    62401 tccaactaaa agctagaagt ctttctatcg gaacattgag tttgccaatc aaagatgtga
    62461 tgaatatgat caagagaaac tataaattac cagattggat cgaagtcaat acagatgatc
    62521 tgactatcat gcttaggttg gatcaattcc gaatgcaaaa cgggatgtat attaaagcag
    62581 ataaaatcga cttagtaaat gatgacattc ggtttagtct atatttgcca aaagatacct
    62641 ctgatgcttc aaacaactca aataccaaca aaactagcac cagcagtact gaaagcaaga
    62701 caaaagaaaa taaaaaatca tcggatgagt agtattagag ggggaatcat aatggaacga
    62761 gcaatatttg caggaggctg cttctggtgt atgatccagc ctttcgacac gcttcctggt
    62821 atccatacag tgatgtcagg atatactgga gggcatgtac cagatccgac ttatgaacaa
    62881 gtaaaaagcc aaacgactgg acatacagaa gcagtggaga ttatctatga tccaacattg
    62941 atttcatatg aacaattagt agaactttac tggcagcaaa ctgatccaac tgacgctttc
    63001 ggacaattcg aggatcgcgg tgacaattat cgcccggtga tcttttattt gacagaagaa
    63061 cagcggatca ttgctgaaaa aagtaagaaa cagttagctg aaagtggacg tttcgaacag
    63121 ccaatcgtta caaaaatcga accagcccaa ccattttatc tagcagaaga agaccatcaa
    63181 gagtattata aaaagcatcc tgaaaatttt aaaagaaacc atgcacgccg ggctgccttt
    63241 atcgaagaaa attggaggaa cgaagggtga atcgaagctt ttttcattat ttaatgacac
    63301 tgcgaggcgg tggcacacat gatgctttat ctgttttcgc tacagaagcc ggtaaagaca
    63361 tccaatttcc taagcacagc tctagctacg aagaaatttc taattatctg gaactgaatg
    63421 tcgactattt gccgtccatg gatatctttg acgaagcatg ggaaaaatat ttagaaaata
    63481 atcatgacat ataagagata gttttgtact ttatgatcaa aaaagttttt tgtccaatag
    63541 cctaattgaa agaattattt ttatttcagc tatctagtcg catttttttg atatttttct
    63601 tataataaag aaagaatgaa aaaaagaggg atggattgat agagattgac aaaaaaacaa
    63661 cgctatatta atcgcttctg gcttgtattc aaatacttac ttgtttctgt attccttgcg
    63721 ttcatgttgc ggggattttt attcatcccg gtaccagtgg aaggcaattc aatggaaaac
    63781 gtgttgaagc aaggtgatat ggttgtgatg gaaaaatttt cggagatcag acgatttgat
    63841 attgttgttt ttcaattagc agatggcacg atttatatca aacgagtaat cggtcttcca
    63901 ggagaaaatg tcagctacca aaatgatcaa ttgaagatca atgggaaagt agttaaggaa
    63961 ccttatctaa ctaaaaacct gaaatcagat catgcaaatg catcctatac gacggacttt
    64021 acactacaag agctaacggg acaatccaag cttccggaag ataattattt tgtacttgga
    64081 gataatcgaa gagtttcaaa agatagtcgt tcgtttggca cgatcaataa aactgatatt
    64141 ctaggtaaag caagatttgt ctattatcca cttgatgaaa taaaatggat ccagtgagaa
    64201 acaataaagc gtgcagtctg ttgagactgc acgctttatt gtttaatcca aaatcagttt
    64261 actaccttag tacgtaaaat caaacagctc gtttaaacac ttttctagcc tctaaagttt
    64321 tgataaatga ataaacaacc attatttgaa tagggatttg gatcatattc ctgatgaatc
    64381 tacctagaaa aattactttg aaatctgcac caaacaaaac agacatcagc caagaattga
    64441 taaaaaggct gactgggatc ataatcaata attgggcagc gatgacggaa ggcaggttta
    64501 tctttttatg atataaaaat aagccattaa taaatccacc aaggaaagcc gtgataagaa
    64561 aagcagcttg aaacgttcct gaaccaaata tcgtgaaatt taaagtgttc gacaacattt
    64621 caagcaaacc agcccagatc ggtccatata gataacccat aagcgccata cttaaaaatc
    64681 cgaatgtgat ttgaaaataa gcaggaccaa tcgctatctt gtataagatg acatcaaggg
    64741 ctaataaaag agcagaggtc gacaacaatc ttgcattcat ttcgggcaat ctaaaaacgt
    64801 cttttgcaat caaagtgttc aaaatattct ctcctttgtc agttaataat aaaaggatac
    64861 cataaaatca ccaatgtgtc atgacacatt ggtgatttac atacactggc atctaacaat
    64921 taaaaaaagg gtgagaagac tgcttttata aactgtaaaa aagagattgt acgaataaaa
    64981 aaaatagagg cggagaatac ttatcgtatc atccgccttt gtttttttgt tataaaaaac
    65041 gtcagattag ttatctgctt acatcttcat ccatacgggt tctaatgtat ttccgtctag
    65101 atcttggact tctaatgaaa acatttcttc ttcaggcatt cccatatcta cttcgtggaa
    65161 agtaccacca ttttcttttg caatttgagc aaattttttt acttcatccg cattttcaac
    65221 actaaacgaa atcaaagcac cacttgtttt tttattatca gctatttctt tatcttttat
    65281 aaatttttta taaaaatcat gagagagaag cattatccaa aaagtgtcat cccataccat
    65341 tgaaccagct tcttcagtag aaaattcttc atttttttta aaacccagct tttcataaaa
    65401 agcaacggaa cgattgacat cagcaacagg aaaattaaca aaaaccatac tattcatttg
    65461 tttcgtctcc tttctgtttt gttcgtctat catctcttat ttaggaaacg ctgtcaaaaa
    65521 tttagattca tatatagaaa ccaaaataaa aatttgcatc atcaaaaaag tgaagatgtg
    65581 atagaatgag gactggttca ctttcactta actaatagat ggaatttctt tataataaaa
    65641 aagtgaattt atattgaaaa agaaaaaaac aagtaaagcc ggggaatatt ccggtatgaa
    65701 aggaatggag agaacaatga ccatccaatg gtttccggga catatggcaa aagcccgacg
    65761 agaagtttct gaaaaaatga aatatgtgga catcgtattt gaattggtag acgcacgatt
    65821 acctttatct tctcgtaatc caatgctaga tcagatcatt cagcaaaaac ctcgccttgt
    65881 tttattgaat aaagcggatc tggcagatcc acaacagaca aagctgtggc aagaatattt
    65941 tcgtaaacaa ggccatcttg cattagctat cacggcacaa gaaagtaaag gaattaaagg
    66001 tctagtacct gcggctaaag aagcattgaa agaaaaacgg gcaagagatg ctgctcgggg
    66061 gatcaagcct cgcgctattc gggcaatggt cttaggtatt ccaaatgttg ggaaatctac
    66121 gttgatgaat cgactagtag gcaagaaaat tgcacagaca ggaaataagc ctggcgtaac
    66181 aaaaggtcag cagtggcttc gtttaggtaa tgagttggaa ttgttagaca caccagggat
    66241 tttatggccc aaatttgaag atgaagaaat cggcaaaaaa ctagcgctga caggcgcgat
    66301 caaagaccag cttgtccatt tagatgattt agcgatttac ggattagact tttttgcccg
    66361 atattattca ggaagaatca gcgatcgtta tggtttatca atcgagcaag aagaaaaatt
    66421 ggcaccggaa ttactgatgg atattacagc aaaacgtggg tataaagaag actatgatcg
    66481 tggcagcgaa ttagtcgtgc atgatattcg tcaaggaaaa cttggacgat acacactgga
    66541 tcgttgcgaa gaaatggagc aaaaaagtga tgactgaatc catccaaagt atcaaagaac
    66601 gattgaaaag catacagaga atagatgatc ctacactaga atcattaaga aatgatccac
    66661 gcaaaggcgt gcagcaagca ctggcttctt ttgaacgacg attgaaaaag gaagcattaa
    66721 ttaaggaaaa atatgaagaa atgttttgct ttgaaaagca agggtacgca caaggtcacc
    66781 aatggattgc cggcattgat gaagtaggaa gaggaccatt agctggacct gtcgtggcag
    66841 ctgcagttat cttgccgaaa aatgagatca tattagggct gaatgactcc aaacaattat
    66901 cagagaaaaa gcgagaactt ctttatcaac aagttcaaga aaaagccctg gcaatcggga
    66961 tcgggatcgt tgatcagcag acaatcgatg atgtgaatat ttatgaagca agtaaattag
    67021 cgatgatcca agcagtagaa aaattgtcca ttgtaccaag ctatttgttg atcgatgcga
    67081 tgaccttgga tttgcctatt cagcaggaaa aaataatcaa aggggatgca cgatctgtgt
    67141 cgattgctgc tgcaagtatc attgctaaag tatatcgaga ccaattgatg aaagaatatg
    67201 acgtgcttta tcctggatat ggtttctcgc ataatgcagg ttatggaaca aaggaacact
    67261 tagaaggact tgaacgacaa ggaattacgc caatccatcg taaaagtttt gcacctgtaa
    67321 aaaaatattt acaataaaaa acagccgctt ttacgtatct tattagaaag agagacgaaa
    67381 ggcggtttta tgatgtatca aatagaagaa aatttattga aacttgctta tacaaaagga
    67441 atcagtagtc aaggaaaatg ggcacttgct aactggatga tgcagtatcc ttataaagaa
    67501 atcggatttg aagacatcat taagataggt aaaataacca aacaccggga gctgtttcgc
    67561 aaaagttggc aggaaattca cgctaactgg ggcaaaatcc aatcgaaaca gtcgtttatc
    67621 acttgctttg atcccaaata tccccctcag ctgcttcact tgacctatcc gccaattgta
    67681 ctgttttata atggtgatct atctttactt tcttgtaaca tgttaagcgt tgttggtgga
    67741 agacacactt ctgctttagc caaaaaaaca gtttattatt tactaaaacc agtgatagaa
    67801 gctggatatg tgatcgtcag tggctgcgcg aaagggatag atacatacgg tcaccaagca
    67861 gcaatctctc atggtggacg gacaatcgca gtcattggaa cagggataga gcaagtctat
    67921 cctgctgaaa atcgtgactt gcaagaggtg attgcaaatg atcatcttct tttgagtgaa
    67981 tatcctcctc aatccggccc gaaaaagcat tgttttccaa tgagaaatcg gattatcgca
    68041 gctttgtcta aaggaacatg tgtctttgaa gccaagaaaa aaagcggctc tctgataaca
    68101 gcacagcaag cattggatct tggaagaaca gttttttctg ttccaggaaa tattttaaca
    68161 gaacattcta ccggatgtca tcatttgatc caagacggtg cagtttgcac ggtttcgggt
    68221 gaagatatct tggcagaatt taaagaataa caccatactt ttcatttgct tttcttgaca
    68281 aacgttttct gaatggatat gattagctac gatttgtatg aatcctcttt taatagtagg
    68341 aacacgacaa tggaaaggag tccattcagt aatggcgtat aaatatttag tgattgtaga
    68401 atcacctgca aaagccaaga caattgagaa atatcttgga cgaaactata aagtaatggc
    68461 cagtgttggc catataagag atttaccgaa aagtaaaatg ggtatcgatt ttgaaaacaa
    68521 ttatgaaccc cattatattt ctatacgcgg aaaaggcgat gtcatcaaaa gcctgaaagc
    68581 cgcagcaaaa aaagctcaaa aagtttactt ggcaagtgac ccggatagag aaggagaagc
    68641 gattgcttgg catttagcgt acctacttgg gttggatctg aaagaaaaaa atcgggtggt
    68701 cttcaatgaa atcacaaaag acgcagtcaa agcagctttt aaggaaccaa gaacgatcga
    68761 tgtagattta gtagatgcac agcaagctcg tcgtacctta gacagaatcg ttggttattc
    68821 gatcagtcct attctctggc gtaaggtcaa gaaagggtta agtgcaggac gtgtccaatc
    68881 tgtcgcttta aaaattatta ttgaccgtga aaaagagatc cgagaatttg ttccagaaga
    68941 atattggagc atcgacggta attttaaaaa agctcgcaag aaattcaaag caaatttctg
    69001 gggaatcgac ggtaagaaaa agaaattacc agatgcacaa agtgtaaaag aagtcactgc
    69061 tagaattgat ggaacagact tcgaagttaa aaaagtagaa aagaaagagc gcaaaagaaa
    69121 ccctgcgaat cctttcacta caagtagcct gcaacaagaa gctgcccgta agttaaactt
    69181 tcgaacaaga aaaaccatga tggtagcaca gcaattatat gaaggaatca ctttgggtaa
    69241 acaaggaaca gtcggcttga ttacatacat gcgtactgat tctacccgga ttgccgatac
    69301 agcaaaagca gaggccgcag catttattga agatacgtat ggaaaagaat tttctagaca
    69361 tgatcaccga aagatcaaaa atgctcaagg cgcacaagat gcccatgaag ctgtgcgtcc
    69421 aacgagtgtg cttcgtacac cagatgaact caaacaatat ttagataaag atcaattgaa
    69481 actttataca ttgatctggt cacgttttgt ggctagtcag atgacaccag ctattttaga
    69541 tacaatgaaa gtgacattag aacaaaatgg tgtcatcttc attgcgaacg gttcaaaaat
    69601 caaattcaaa ggattcatgc aagtgtatgt ggaaggtaga gatgacggca aagaagagaa
    69661 agaaaatatt cttccagaac ttgaagaagg cgatgtagtc caatctgttg atattgaacc
    69721 aaaacaacat tttactcaac caccagcacg ttttagtgag gctacgttga ttcgtgcact
    69781 agaagaaaac ggggtagggc gaccttccac ttatgcacca acacttgata cgatccaaag
    69841 acgctactat gtaaaattga cacaaaaaag atttgagccg actgaactag gagaaattgt
    69901 caattcatta atctgtgaat tcttccctca aatcgtcgat atccatttca cagcagaaat
    69961 ggaaggagat ttggataaaa ttgaagaagg cacggaagcg tgggtcaaag tggttgatcg
    70021 tttctataaa ccgtttgaaa aagaactgac gaatgcggaa gaaaaaatcg aaaaaattca
    70081 aatcaaagat gaaccagcag gattcgattg tgatgtctgt ggacatccga tggtgataaa
    70141 attagggaaa tatgggaagt tttatgcttg cagtaatttt cctgattgtc ggaatacgaa
    70201 accaatcgta aaagaaatcg gtgtgacttg cccagtatgt catgaaggac aagtcattga
    70261 acgaaaatct aagaaaaaca gactattcta tggttgttcc cgtttcccag aatgtgaatt
    70321 cacttcttgg gataaaccag tgggcagaaa ttgtccaaaa tgtgacggat atctggtcga
    70381 gaagaaagtc aaaggcggta aacaagtagt ctgtgtaaat ggagattacg aagaagacat
    70441 ccaaaaataa tagaaaagag gttctttcat tgaaaatggt aaatgttgtt ggagcagggc
    70501 ttgccggaag tgaagctgct tggcagatcg cacaagcagg agtccctgtc cgtttatatg
    70561 aaatgcgtcc agtcaaatcg actgaggcgc atcacacaag taattttgct gaactagtat
    70621 gttccaattc attaagagga aacagtttgg ctaatgcagt agggttattg aaagaagaaa
    70681 tgcgccgatt gaattcggta gtaatcactt cggcagatga aacagcagtg ccagctggtg
    70741 gtgccttagc tgttgaccgc gattcttttt ctgaaacaat tacccgaaaa gtcaaagagc
    70801 atccattagt aacagtgata aacgaagaaa ttactgaaat accggaagga atcaccgtag
    70861 tagcaacagg acctttgact tctcatccat tagcagaaag catcaaagca ttcaatggtt
    70921 cagatggttt ttatttctat gatgcagctg caccaatcgt agacaaagct acgatcgata
    70981 tggataaagt atatttgaag tcccgctatg acaaaggaga agcagcttac ttgaactgtc
    71041 cgatgacaga agaggaattc aaagctttcc atgaggcact aatcaatgcc gaagtggcag
    71101 aacttaaatc ttttgagaaa gaaaaatatt ttgaaggatg tatgccaatt gaagtaatgg
    71161 cacagcgagg attcaaaacg atgcttttcg gacctatgaa accagttgga ttggaagatc
    71221 ctaaaactgg caaacgtcct tatgctgtta tccagctgcg tcaagacaat gctgcagctt
    71281 ctttatacaa catcgtgggc tttcagactc acttgaaatg gggcgagcaa aaacgtgttt
    71341 tccggatgat tcctgggtta gaaaatgctg aattcgttcg ctatggtgtg atgcaccgaa
    71401 acagctttat gaattcgcca gaattgctga aaccgactta ccaatcaacg aaacgcgaag
    71461 atctgttctt tgcaggtcag atgactggtg tagaaggcta tgttgaaagt gcagcgagcg
    71521 gactacttgc tggaatcaac gccgcacgct tagcaaaaga aatggaacca gtcgagtttc
    71581 cacaagaaac agctattgga agtatggctt actacattac tcatgcggaa ggaaaacatt
    71641 tccaaccgat gaatgccaac tatggtctgt ttcctgaact gccagagcgc attcgagaca
    71701 aaaaagcaag atatgaagca attgcacaac gagctttaaa agctaatgag gcaataaaaa
    71761 cagaattaga aaaaactgca atcccaaatg agtaaggaaa ctccatgaag agggtgagac
    71821 ggatagtctc gctctttttc ttgcgttttt cttaagaatt tctcaaacaa aatgtgagga
    71881 attgcgccaa ttgtgaacaa acttagaaat attggttaaa ttctgacaat ttatttgtct
    71941 ttttataaac gctgtgctaa attattctct gggacaaaag gaaggtgaga aaatgacaga
    72001 agccacgtgg gaacaaaaat ttttgcgtta tttgattgtc gaaagaggat attcagaaaa
    72061 aacgaaagaa gcatatgaag aagacattct ccatttcaaa cattttttgg aggaatctgg
    72121 agacgctgat ctgctgaagg cggaatattt tacggttcgt gtctatttga gtgcattagc
    72181 tgatcatcgt cctagctata gcagaaattc tatcagtcgc aagatagcca gcctacgttc
    72241 gttttatcag tatcttctca aagaagaagt gataaaagaa aatccatttt cttacgttca
    72301 tctcaaaaag aaaaatttga gactgccacg ttttttttat gaaaatgaaa tgcaagcact
    72361 ttttgacagc gtagcaggag atagtccttt ggatttgagg aaccgcgctt tactggaagt
    72421 cctttacggg tcaggcatcc gtttaagtga gtgtagcagt ttgtcggtat cagacattga
    72481 ttttgattca gaggtcatgt tgatccatgg taaagggaac aaagagagat atgctcctct
    72541 aggttctttt gctcaagatg ctctgcaaga atatttcagg gaaggccgcc aggtattgat
    72601 gacaaaatat cacgaagagc atgatatcgt ttttgtgaat catcatggaa aaccaatcac
    72661 gcctactgga attgaatatg tcttgaatca aatgatcaaa aaaagcagtt tggatagtga
    72721 tatccatcct cacatgctgc gccatacatt tgctactcat ttattaaata atggggcaga
    72781 tttgcggaca gtccaagaat tgctaggaca tgccaattta tcgactaccc agatctatgc
    72841 ccatgtaaca aaagaaagtt tacaaaaaaa ctatcgttct ttccatccaa gagcgtaaaa
    72901 ataaggaaaa gaacaaattt aggaggaaac aaacatggtc gaatcaccat ttcattcaac
    72961 cactatctgt gccgtcgaaa aagacgggaa atttgctatg gctggagatg gccaagtaac
    73021 aatgggtgaa tctgttgtca tgaaaggcac tgcaaagaaa gtacgacgta tctacaatgg
    73081 agaagttgtt gtaggtttcg ccggaagtgt tgcagatgcg tttactttag aagaaaaatt
    73141 tgaaggcaaa ctaaacgaat ataacgggaa tctacaacgc gcagctgtcg aattagctca
    73201 agaatggcgg acacagcaat ctatgcaaaa acttgaagcg atgctgatcg taatgaatga
    73261 taaagaaatg cttcttgttt caggtacagg agaagttatc acaccagatg atggtatctt
    73321 agctatcgga tcaggaggga attttgcgtt ggctgcagcc cgagcaatga aaaactatgc
    73381 tcaaaacgac atgagtgcga aagaaattgc agaaaatgcg ttaaatattg ccgcagatat
    73441 ctgtatcttt actaatcata atattatcgt agaagaactg taagaggtga gtagatatgt
    73501 atgaattaaa acaaacacca agacaaatcg taagtgaatt agatgaatat atcgtaggac
    73561 agcacgcagc aaaaaaatct gttgccgttg ctttgcgtaa ccgctatcgc cgtttgcagt
    73621 tagatgaaaa aatgcaacaa gatattacac caaaaaatat gctgatgatc ggacctacag
    73681 gggtcggtaa aacagaaatc gcacgacgtt tggcaaaaat cgtcaatgca ccatttgtca
    73741 aagtcgaagc aacaaaattt actgaagtcg gttatgtcgg aagagatgta gaatcaatgg
    73801 ttcgggattt agtagaaaat gcgatccaaa tcgtagaaaa agagcagtac agccgtgtat
    73861 acacaagtgc taaaaaaaat gcagaaaaac gtttggtaaa aatcttggta cctggtatca
    73921 aaaaagaaca gaaacaatcc actaatcaat ttgaacaaat gatgaatctt tttaatgcta
    73981 gtcagcaaaa taccgctgaa ccgcaagaag aagtgacaga agatattcga atcaatcgcc
    74041 gtacagtctt ggaacaacta caaaagggtt tactggatag tcgtgaagta accattgaag
    74101 ttgaagacca aaagaaacct gcaccaatga tgaacaatgg attggagcaa atggggatcg
    74161 acctaaatga aactctaggt gctttgacac cgaagaaaaa agtggaacgg acggtaacag
    74221 tcaaagaagc tttagagtta ttgatcaagg aagaatcagc aaaattagtc aaggatgctg
    74281 atatccatag tgaggcaatc aaattagcgg aagaaagtgg aattattttt atcgatgaaa
    74341 tcgataagat cacttctaaa tcacagcaat ctggagaagt atccagagaa ggcgtccaaa
    74401 gagacatatt gccaattgtc gaaggttcac aagtaaacac aaaatacggg atcatccaaa
    74461 cagatcacgt cctattcatt gcttcaggag ctttccattt gtctaaacca agtgatctaa
    74521 ttccagaact tcaaggacgc ttcccgatac gggtagaatt agatgatttg acagctgaag
    74581 attttgtgaa gattctgacc gaaccaaata atgctttagt aaaacaatac gtcgcgttga
    74641 tcggtacaga aaatgtgtca gttacgttta caaaagaagc aattgaacgg atcgctcaaa
    74701 tcgctttcga tgtcaaccgt gacacagata atatcggcgc acgcagactg cataccattt
    74761 tggaaaaact actagaagat ctattatttg aagcaccaga tatgcaaatg ggagaaataa
    74821 cgatcacaga agcatatgtg gatgacaaac ttggctcaat tgcggcaaat gaagatttaa
    74881 gtcgctatat tttataaaaa atcggaggac gacaatgaca gacttgctaa cgaaaacccg
    74941 caagataaac acacttttgc agcaaaagaa tacttttgac atgcaagcag atttgcctta
    75001 cgataaaatg gctgtcacac taggtggtat tcttgacagc aacacctata tcataggaag
    75061 tgacggagta ttactaggtt ataatgaacg tcacgatgtg aacaatgaac gaatcaaaac
    75121 aatgtttgtt gagaaacaat ttcctgaatc ttatactgat acagtggcac agttgatgaa
    75181 aactgaagct aatatcccaa ttactagtga tattactgca tttccggtag agtcaagaga
    75241 gaaatatcca tttggtctga cgactgtcgt tccgatcttt ggagctggcg aacgtttggg
    75301 tacgattatc ttatctcgta tggaacaatc gtttgcagat gacgatctag tcttagcaga
    75361 atatggagct acggttgtcg gtatgcagat cctttatcag aagtctcgaa gcatcgaagc
    75421 agatgtacgg agtgcaacgg ctgtccaaat ggcaatcaac acattatctt atagcgaatt
    75481 gaaggctgta caagctatct ttgaagcatt ggatggagat gaaggtcgtc tgactgcttc
    75541 taatattgcc gatagtatcg gcatcactcg ttctgtgatc gtcaatgctt tacgaaaatt
    75601 ggaatcagct ggaatcattg aatctcgttc gttaggcatg aagggaactt atttaaaagt
    75661 attgaattcc cgttttaaag acgagttagc aaaacacagt tattagaagt aaggggactg
    75721 gggcagatag aaatgtttgt tccagccttc ccattttaaa aagtaagtcg aagttggagg
    75781 aatggcaatg actgttatta ttcaaaatga ccagctgatc gccgaaatcg ctgaacacgg
    75841 tgcggaattg atcagtctga aatcaaaaga acatggttta gagtatatct ggcaaggtga
    75901 tccagcgtat tggggcagac atgcaccggt tttattccct tttgttggac gtttaaaaga
    75961 tgatcagtat gtttatcaag ggaaaacata tcaaatgggg caacacggat ttgctcggga
    76021 tatggatttt gaagtcattg aacatggaca agaaatggct agtttcatct taaggagtac
    76081 gccggagaca ttagaaaagt atccatttga ttttgaatta gtgatcagct atgaattagg
    76141 aggagacggc atcacggttc attatcaagt ggcaaatacc ggtgaaaaag aaatgttctt
    76201 ttctatcggt ggacatcctg ctttcaatgt acctcttgaa gaaggattgt catttgaaga
    76261 tttctatctg gcattttctc cattgaaatc aagattacgc ctaccattga aaggcccatt
    76321 catcgatatg gatcaaaaga cgttaggtca gacaaatacg aatctggctt tgaatcgtga
    76381 actttttgag caagatgcct tggtttttga aacaaggggt ctaaatgcgt acgccattcg
    76441 aagtgaaaaa tcttcccata gcgtaacttt aagctataaa gatatgcctt atgtagggat
    76501 ctggtctcct tatccaaaag aagcgccttt cgtttgtatc gaaccgtggt gcggaatcgc
    76561 tgacacggtg aacagtagtg gaaacttgat ggaaaaagta gggatcaatc aactaggtgc
    76621 ccatgaaatc ttcaagacaa aatattccat cacagtgaag taagaggttg tgaccgagaa
    76681 gttagcaggg aacaccgttt accaaggtgt gtaaaaaagc tatcctgtaa gtaataaggg
    76741 agtgtgacat cgatttgtca cactcccgtt ttatctcttt ggaatgaaaa agtatcagga
    76801 ttttttcttg ctgctattca aaccaaacgg gattcgactt tcagtatggt ttctgatacg
    76861 cttgatattg tctttatgcc gataaaagat aaaggaagaa actaaaaagg caatgatcgt
    76921 cagtaaccaa ttgggcttag ccaaaatcac aggagctaca taaggaagga cgatcgtaga
    76981 taaagtgatc aatactgcac tgatcatact gctcaaactc accatacttg tacaaaataa
    77041 actaacgata aagatgatac aagaataaac aaaaaataca ggattaaatc ctaaaagcat
    77101 tcctgcactt gtcgcaacag cttttcctcc tttgaaccca gcaaaaatcg gaaatgtatg
    77161 gccaagaatg gcgcataccc caaaccaaag agggttaatc ccagaaaggt ggaagatcat
    77221 cggcaaacaa gtagccaatg taccttttaa aatatccata agcaggacaa caatcccggc
    77281 tgttttgcct aatacacgaa atgtattggt cgttccagta ttaccgctcc cgtgttgtcg
    77341 gatatctttt ttgaaaaaca atttgccaat ccagacaccg gaaggaatag aacctaaaag
    77401 atatgctaaa ataagtaata agatcaattt cattttttac cctctttcaa gttgaaaact
    77461 atcctttatt ttagcatgaa taggtaacag gaacaattga aaaaatgaaa gatacaagta
    77521 taatagatgg aataaaaaga aaagcaaggg gtggcagaaa tgtctgtaaa aaatccaaca
    77581 caagaaacga ttttcaacta tttggaagaa gcaaaaaata ttgccgtcgt aggcttgagc
    77641 aataaaccag aacgaacaag ttacatggtt gcacaggtat tgcaagacaa tggatataaa
    77701 atcataccag taaatccatt attggctggt gaagaaattc taggcgagaa agtctatgcg
    77761 aatttgacag aaatcgatga tccaatcgat atcgtagatg tatttagacg gagtgaattt
    77821 ttacctgaaa tagcaggtga ttttttgaaa actgatgcta aagtgttctg ggcacaatta
    77881 gggattgaaa acaaagaagc agctgagatg ctaagaaaag caggacgtac agatatcgta
    77941 atggatcgtt gtattaagat tgaacttggt aagttgaaag aaaaccaaaa ataaaaatga
    78001 ccacaaagaa gagatgatcg atagagggga atgtcgaaaa agcggttagg agataattaa
    78061 aattgatttc tcgtctaccc tctttttttt cacgaccttt tctagtatga taatcgtggt
    78121 gcggttttgc cgcaaagtat tattgaagga gcgtttgcct tggcaaaaaa agtaaacaac
    78181 gaatacaatg actcgtccat ccaagtattg gaaggattag aggcagttag aaaaagacca
    78241 gggatgtaca ttggatccac tgacagccga ggcttgcatc atttagtcta tgaaatcgtc
    78301 gataatgcag ttgacgaagc attatcaggt tatggcagtg agattgatgt gaccatccat
    78361 gaagacaata gtataactgt tgcagatagc ggacgtggga tgcctgtagg aatgcatgct
    78421 tccggaatac caacagttga agtgattttt actgtgctgc atgctggcgg aaagtttggt
    78481 caaggagggt acaaaacttc tggtggactg cacggtgtag gagccagtgt ggtaaatgcc
    78541 ttatcgaaat ggctaattgt aacgatcgtt agagatggcg tagagtatca gcagaaattt
    78601 aaaaacggcg gtaaacctga cggtactttg aaaaagatag gaaaaacaaa aaaagcaaac
    78661 gggacgactg tccatttttt accagatgat acaatattca gtacaacgaa attttcatat
    78721 gaaattttag ccgaacggtt aagagaatct gcctttcttc taaaaggcgt taaaatctct
    78781 ctttctgatc ttcgtggaga agaaccagtc aaggaaattt ttcattacga agaaggaatc
    78841 aaagaatttg ttgattatct gaatgaagaa aaagatacat tgacaccagt tgtttatttt
    78901 tcaggagaaa aagaaggtat cgaagtagag gtcgcttatc aatacaatga cggttattca
    78961 gaaaatgttt tatcttttgt caataacgtt cggacaaaag atgggggaac ccatgaagct
    79021 ggtatgaaag cggcgatgac gaaatcttat aatgaatatg cgagaaaagt tggtttgctg
    79081 aaagaacggg acaagaatct ggaaggtagt gatttcagag aagggttagc tgctgtactt
    79141 tctatccgtg tacctgaaaa tcttctgcaa tttgaagggc aaacaaaaga aaaacttggt
    79201 acgccagttg ctcgtaccgt agtagataac gtcatcagtg aacaaatggg cttttatttg
    79261 caggaaaata gcgaaatgag ccagatgttg gtccgtaaag caatcaaagc cagagaagcg
    79321 agagaagcag cacgtaaagc cagagaagaa agccgaaatg ggaagaagcg taaaaaaggc
    79381 gaatctcttc tttcaggaaa attgacaccc gcacaatccc gaaaccctaa gaaaaatgaa
    79441 ttgtatttag tcgaagggga ttctgctggc ggatctgcta aacaaggaag agacagaaag
    79501 ttccaagcaa ttctaccttt acgaggaaaa gtcatcaata ccgaaaaagc taaaatgcaa
    79561 gatatcttga aaaatgaaga aatcaatacg atgatctata cgattggtgc aggagtcggt
    79621 cctgagtttt ctatcgaaga ctgtaattac gacaaagtaa tcatcatgac cgatgcggat
    79681 acagacggcg cccatattca agtattactg cttactttct tttatcgtta tatgaaaccg
    79741 ctgattgaag ctgggaaagt atatatcgcg ttaccgcctt tatataaagt atccaaagga
    79801 caaggaaaga agcaagtcat tgaatatgcg tggacagatg acgaattggc agcaatgatt
    79861 aaaaaagtcg gcaaaggata tatgctgcag cgatataaag gacttggaga gatgaatgcg
    79921 gaacaattgt gggaaacaac gatggatccg acttcccgta cgttgatccg cgtccgtatc
    79981 gatgatgctg cgcaagctga acgtcgagta accacactga tgggtgacaa agtagaacct
    80041 cgaagaaaat ggatcgaaaa tcacgtgcaa tttacgctcg aagaagatgg cagtatctta
    80101 gataaaaaag aggatactga aatctctcct tcagtttcta atgatttatt agatgaagaa
    80161 cgagcagata aaaatgaaaa caatcagtta tttgaagtag aataggaggt cacctgatgg
    80221 aaaatcgaca agaagtacaa gaattaactt tagaagaagt gatgggtgac cgcttcggac
    80281 gttattccaa atatattatc caagaacgtg cactgcctga tatccgtgat ggattgaagc
    80341 cagtgcaacg ccggatatta tttgccatga acaaagatgg caatacgtat gacaaaggat
    80401 tccgtaaatc agcaaaatct gtcggaaata tcatggggaa ttatcatcct catggtgaca
    80461 tcagtattta tgaagcgatg gtccgcatga gccaagactg gaagttgcgg gaagtactga
    80521 tcgaaatgca cgggaataac gggagtatgg acggtgatcc acctgctgcg atgcgttata
    80581 ctgaagccag attatccaag ttaagtggag agatgctagc agatatcgag aaagaaacag
    80641 tcgatctcgt gtggaatttt gatgatacag aaaaagaacc aacagtcctt ccagctcgat
    80701 acccaaacct tctcgtcaat ggatcgacag gtatatctgc tggatatgca acagaaatcc
    80761 cgactcataa cttagctgaa gtgatcgatg gcacaatcta tatgatcgat catccacaag
    80821 cgagccttga aaaattgatg gaatatattc ctggacctga ctttccaaca ggagggatct
    80881 tgcaaggaaa agatgaaatc aaaaaagctt atgaaacagg acgaggaaaa gttattcttc
    80941 gttcgaaaac aaagatagag gcaatgaagg gtaataaaca gcaaatcgtg atcacagaaa
    81001 tcccatatga agtaaataaa gcgaccttgg tgaaaaaaat ggatgagatc cgtttgaata
    81061 agaaaatcga tggaatagct gaagtacgag atgaaagtga tagaacaggt ctgcaaattg
    81121 tcgtcgaatt gaaaaaagac gccaatgctc aaggaatttt gaattatttg ttcaaaaaca
    81181 ctgagctaca gatcaattac aatttcaata tggtcgctat cgaccatatg actccgcatc
    81241 aagttggact gaaagacatt ttaagaagtt atatcgagca tcgaaagcaa gtcataacaa
    81301 aacgtagtca attcgattta gccaaagctc aaaaaagaca gcatatcgta gaaggattga
    81361 tgaaagcttt atcgattctg gatgaagtaa tcgcaacgat tcgtgaaagt aaagacaaaa
    81421 aagacgccaa aaagaattta gtcgacgtct ttcaatttac agaagaacaa gcagaagcaa
    81481 ttgtcacatt gcaattatac cgtttgacga acacggatat cacagagctt caaaaggaat
    81541 ccgaaagctt gattgcgcaa atcacagagt tgaataaaat cctttcaaat gacaaagagc
    81601 ttttctctgt tatgaaaaaa gaattgcgag aagtgaaaaa aaattattct tccgctcgtc
    81661 taacgacaat cgaagacgaa atcgaagaaa tcaaaattga tacgcaagtc ttagtggcac
    81721 aagaagacgt aattgtcagt gtgacgcgag aaggctatgt gaagcgaaca agccttcgtt
    81781 cttatagtgc ttccaaacca gaagaaatcg ggatgcgaga aggcgactac ttgctatact
    81841 caggtgaatt gagtacattg gaccatgttc ttctgatcac aaataaagca aatgtgatct
    81901 accgccctgt ccatgaacta cccgatttga aatggaaaga tgctggtgag cacatttctc
    81961 aaacaatctc caatctgtct gtagacgaat cgattttagc tgttttccca tatcaaaaaa
    82021 ttcaagcaga gaaaacattt gtattcatca gcaagaatgg attgatcaag caaacacgaa
    82081 tgacagagtt cgaaccatgg cgcacctata agagccgtcc gctcagctgt atgaagctaa
    82141 aggcggaaga agatgaactg attgctgtgt atctgaaaga agatcagcca ggcttagatg
    82201 ttttcttagt aacgcatcaa ggaatgggtt tgcgctatcc tttaacagat gttcccgttg
    82261 ttggagcaaa ggcagctgga gtgaagtcta tgaatttgaa agatggagat aggattatca
    82321 acgggctgct tgtgttagca gaaggtgata cgccgatcgt aatcgttact cagcgaggag
    82381 ctgttaaacg catgcttgct caagaaatca gccaaacttc acgtgcgaag cggggagtga
    82441 cggtcatgcg tgagctgaaa aaaaatccgc accgtattat ctatatgtcg gaaggaagat
    82501 caaaagaaat aaccttgatg aatcaaaaag ggcagcagtt aacgattgac ccaactgatt
    82561 tccctatagg tgatcgaaca tcgaatggat catttgcgat ggatgagaaa aaaggtggag
    82621 aagtcatcga agttatcgat gctcctgaga ttttcatcga gtcgtaactg ttgtagtatt
    82681 ttaatgaaga gacgccatct gtattaatac aggtggcgtc tctttttcat tcatttttaa
    82741 taaatcctta tacgtttgca aaaaaagatg aaagtgttca caatgcttaa atgttggtat
    82801 gtcaacgttg atttaaatat atgtaaataa aacataaaaa ttgttaaata aaaaacaaga
    82861 aaacccttgc aaagataggg tgctgtgtta tactaatcac gtaaaaactg ttacataaaa
    82921 cagaaattta ggaggtccat acaatggagc aatggaatgg atttaaaggt accaaatgga
    82981 aagaaggcgt agacactaga gactttattc aaaataacta cactgaatat cgcggtgatg
    83041 acagcttttt ggaaggccca acagaagcaa cagaaaaatt atgggatgaa ttgcaaaagt
    83101 taaatgatat ccaattcgag cgtaacggtg tctatgatat ggacacagaa atcgtatcaa
    83161 ctattacttc acacgatgca ggctatttgg ataaagattt ggaaaaaatc gtcggtctac
    83221 agacagacaa acctttgaaa caagcattca tgccgttcgg cggaatcaat atggctaaca
    83281 atgcgctaaa aagcaatggc tatacaccaa acgatgaatt gacacatatt ttcactgact
    83341 ggcgcaaaac acataatcaa ggtgtatttg acgcttatac tgctgaaatg cgtgctgctc
    83401 gtaaaaacaa aatcattaca gggcttccag acgcttacgg tcgcggacgt atcatcggtg
    83461 actatcgtcg tgtggcattg tatggaatcg actatttgat ggaacaaaag aaaaaagatc
    83521 atgatatgac tggttatcat acaatgagtg aagaagtgat tcgtttacgt gaagaaatca
    83581 ctgaacaata tcgtgcatta gcgcaaatga aagaaatggc tgcgaaatat ggctatgata
    83641 tttctaaacc agctgcaaat gcaaaagaag ctgtccaatg gctatacttc ggatatttag
    83701 cagcaatcaa atcacaaaat ggtgctgcga tgtcaatcgg acgtatctca gcattccttg
    83761 atatctatat ccaacgtgat ttggataatg gagtaatcac agaaaaagaa gcacaagaat
    83821 tgatcgacca cttagtaatg aaattacgta tggtcaaatt cgcgcgtaca cctgaatata
    83881 accaattatt ctcaggattc cctatctggg ctactttatc cattgcaggt atgggattag
    83941 atggtcgttc attagttaca aagaatgatt tccgtctgct tcatacatta acaaatatgg
    84001 gaccttcacc agaaccaaac ttgactgttt tgtattctga acaccttcca gaaggattca
    84061 gaacatatgc tagcagaatc gctatcgaaa gttcttcgat tcaattcgaa aatgataatc
    84121 agcttcgtga acaatggggt tctgatgatt gtgcgattgc ttgctgtgta tcaggaacag
    84181 ttgtcggaaa agacatgcaa ttctttggcg ctcgtgctaa cttggctaaa acactattat
    84241 atgccatcaa tggcggagtc gatgaagtga ctggcgccca agttggacca gaatatcgtc
    84301 caatcacatc agaaatacta gactttgaag aagtgaaaca taacttcatg aatatgatgg
    84361 attggttagc agaactatat gtcaatacgt tgaacatcat tcattatatg catgataaat
    84421 attcttatga agcagctcag ctggctttga tggattctga cttgcaacgc acatttgcta
    84481 caggtattgc tgggatctct catgcggcag acagcttgtc agcgatcaaa tatgcaaaag
    84541 taaaagcaat ccgtaatgaa aaaggaatag cagttgattt tgaagtggat ggcgacttcc
    84601 ctaaatacgg aaatgatgat gaccgtgcag atgaattagt ttcatggatc ttggaatact
    84661 tcatgggaca aatcaaacgt cataaaactt accgcaactc aaaaccaaca acttcattat
    84721 tgacgattac ttcaaatgtt gtttacggta aaaatacagg aaatactcct gatggccgtg
    84781 aagctggaaa accacttgcg ccaggagcaa atccaagtta caatgcggaa caaagtgggt
    84841 tattagctag cctgaactca acagctaaaa tgaattacgg ctgtgcattg gatggaattt
    84901 caaataccca aacaatcaat ccttcaggtc ttggaaaaga acaagataca agaatcgaaa
    84961 acttgcgtaa tgtcttagat ggatacttct caaaaggagc ttatcatttg aatgtcaatg
    85021 tattcagtaa tgatttattg cgtgatgcaa tggaaaatcc aatgaaatat ccgaacttga
    85081 cgatccgggt atctggttat gcagttaaat tccgtgactt gacaagagaa caacaattag
    85141 atgtcttgat gagaacttca cacgacagaa tgtaaaaagt tcgtgaaaaa agtttttcga
    85201 cttagcgtcg aaaggacgtt gaatagccat cgcatcaaaa tcgaataatc agtatgctta
    85261 tttgaaagaa acgatttaca ggggcaggac ttgtatgttc cgtccctgta aattttacac
    85321 ataacgaagc ggcaattgcg aaaaattttg ttcagaaagc ttcgcttcga cagctaactt
    85381 gtacttaaaa tgaaatcaaa gaaaagatgt gaacttaaat ggaagaaaaa acaatcggct
    85441 atgttcattc tatcgaaact ttcggttcag tagacggacc tggactgcgc tttgtcgtgt
    85501 ttatgcaagg atgccgcatg cgttgccaat tttgtcacaa tccagatact tggaatattg
    85561 gcggagggaa agaatacaca gcagatgaac ttttagataa agcagaacgc ttcagacctt
    85621 actggggaga taaaggtggt attactgtta gtggtggaga acctctattg cagatcgatt
    85681 ttttgatcga actatttaaa aaagcaaaag agcggaagat gcatactaca ttggatacat
    85741 gcggaaaacc ttttacgtat gaagaacctt tcttttcacg ttttcaagaa ttgatgaagt
    85801 atacagattt actgttattt gacatcaaac acatcgataa tgaagagcat aaaaaactaa
    85861 cgcattggga caatgaaaat attttagaaa tggctcaata tttgtcaaaa atcaacaaac
    85921 cggtttggat tcgccatgta ctagttcctg agcgaagtga ttatgatgaa tatttgatcc
    85981 gactagacaa ctttattcaa accttgtcaa atgtagacag agtagagatt cttccttatc
    86041 atacgatggg aaaatacaag tgggaaacac ttggtttgaa atatccatta gaagggatcg
    86101 aaccacctac aaaagaacgt gtcgaaaatg ctaaacgatt gcttcataca gaagagtata
    86161 caaaatattt aacgcgatag gtcgcatcgg aaagaagata agtattgctt ctttccgttt
    86221 ttttgctatt attaaatagg agaaatcaca aaggagcgaa tgacatgtca aaaattttag
    86281 tttttggtca ccaaaatcca gatacagatg caatcggggc agccattgcg ttttcacact
    86341 tgcaaaatga attaggtaaa gaaacagaag cagttgctct aggtacacct aatgaagaaa
    86401 cacaatacgc attagaccac tttcagctat ctgcaccacg agtagtagag tcagctaaag
    86461 cagaaaatgc agatgtcatg cttgtggatc ataatgaatt ccaacaaagt atttcagata
    86521 tcagcgaagt aaatatttta gctgtagtag accaccatcg tattgcaaac tttgaaacag
    86581 ctaatccatt gtactatcgt gcagaaccag ttggatgtac aagtacgatt attttaaaac
    86641 tatataaaga aaaccaagta acaattccta aagaaatcgc aggaatcatg ctctcagcta
    86701 tcatttcaga tacattgttg ttcaaatcac caacatgtac gcctgaagat gaagcagctg
    86761 caaaagaact tgcagcaatc gctgaagtcg atctaaacac ttatggactt gaaatgttga
    86821 aagcaggaac aaatctaagt gataaatcag ctgcggttct tcttgatttg gatgcaaaaa
    86881 gtttcccaat gggcagttcc aacgttcgta tcgctcaagt caatacagtg gaccttaatg
    86941 aagtgatgga tcgacaagca gagctagaag cagcaatgga agatgaaatg aaagcaaatg
    87001 gctacgatct gttcgtatta gttgtcacaa acatcttaga cagtgattca gaattacttg
    87061 ttgctggtaa aggaaaagaa aaagtagaag aagctttcca aacaactttg accaacaacc
    87121 gtgctttgct taaaggtgtc gtttcacgta aaaaacaagt cgtacctcaa ttgacggctg
    87181 tatttagcta atcaaatggt agtatgaagc attcaaaatg aatggataag cgattgatat
    87241 aaaaagaaac atagaacata ctaaaggccg aagaaatgga ggagaataac agtcaattat
    87301 gactcttgtt cttcctcctt tttacgtaaa agccttttaa gtaaaataaa acggatagaa
    87361 aagagaacat atatgaacta ttattcaaaa aataaggaaa acttttatca aaaactgacc
    87421 gatgatccgc ttttttcttc attgaccgat tatctatatg aacatcgaga acaagaaacg
    87481 attctaagag aattgaaaaa agaattttcg caaaataaat tttcccattt tcttgattta
    87541 ttgatcgatg caggattaat caagagggaa gaacgaagat atcatttgaa ctttccaata
    87601 tttgatccta aagattactt gcaacaagcc acttcggctg ctgaaaccat agctgaacaa
    87661 ttgaagagac tctctgttga tgaacaaaaa ttagcgatgg gagaagttat ttgggcgtat
    87721 tgtttcgaag atgaacggaa agaagcgtat ttttatggag taagaaacag ccgagagaca
    87781 gaattgctga ggacaactgc aggaaatgag aagtaccgtt ttatcacact ttcctctata
    87841 gaacacttcc cgcttacttt ggctaattac ttttttgtac agaagaatca gcttcctgta
    87901 acgaaggcat tcaaagaatt agccgaactt attggagacg taaatgaatc gtactttttt
    87961 gatcaaatag aagttatcgt tgacaggata cgtaagaata aatataaaaa tcgccggcct
    88021 agtattttcc atcagtctct acttgtgacg aatactataa aggaagaaga atcattcaca
    88081 ttagaattac ctattgtcga aaaaaacaat tttgagattg aattaccaac attggatcct
    88141 tcattgacaa tggaggaaat ggcctttctt aaacgacaaa tcttttctga gctgagcaag
    88201 aaatttatac cgcatgcatt ttcttatatc aaagaatatg gaacagtttt agtgagtaaa
    88261 acctaagaga gcagtaagca aaaattatac tgactcttgc aggaggagcc tttctcttcc
    88321 tttatggtta aaagaattat ttgagtattt tgcctatcta ccacctatta gattcactac
    88381 atggtctgga aacaaaggaa tgcaaaaaaa tggtgagaaa tgtggaatga caaaggaagt
    88441 agtgataaga aaagttagat ttttaaataa tcaatattat gattcagtca agtatggcat
    88501 tttatgggaa gaactagctg attagattag gaagtccatt tgataatcca gaatcactaa
    88561 gaaacgaatg gcgagtcatt aataatttat ccaatcttgg gttattcttt tagattgaag
    88621 tgtttaaaac taaaaaagca tttatgaagt cgtaatagtc aggcttcaaa atgcttttta
    88681 ttttgtttgt tttttctttg ccacataaat acacctagac tgacgatgaa gagcatatcc
    88741 aacagatttc ctgcgacttg gatcgtcgaa gggttggtca gtggcattaa agcaaggaca
    88801 aaaaaggaaa acaagctgta aataaaacag gtgacaggaa ctaatcgaaa gaagagcgga
    88861 catttcaatt ggctaaaaat gagtgaaaaa ataaaataag taaaaatgga taaaatcacg
    88921 actaaaccat tcatgataaa gaagaaaagc gtattgtgat taaacgaata aaaaagaatt
    88981 gctaaaaaag aaaacgtttt aaatgcaatc aatacgcttg aagcttcgtt taacgtacca
    89041 ggatgtcgat gaaaaaaata ggcaccgaaa taaacagaaa acagtaagcc gcaaagagaa
    89101 gacaaaatca aaaaaaacgg attttcttcg gcttctgtga tatcagaatg atccaaaagc
    89161 aaccagccta atgcactaga aggcgcatgt gaggtcttaa ggtatgaata acatagaaaa
    89221 aataagatcg tcaaagttgc aaaaaaagag cctaaatgta aaaaattagt ggttctacgt
    89281 tgaatttcca taatatctcc aaattaaatt gataagtata taaagtataa taacaaattg
    89341 atgagtatat gtcgatgttt tttaatcaaa ataataaata aaaactgtct aacatttaaa
    89401 atttaattta gatattagac agtttttaag tttgtgagtt attttccttg tcgttttctc
    89461 acttttttag tccagaaacc acctgaaata tattttggct cataagaaat gataaaagca
    89521 cgctcttcga tttctttgat caggcgatac agttcacgtt cgctttttct aggagaaagt
    89581 atttcgagaa ctagccgctc accttccagc cccattgcag aagtctgtgt cacaccataa
    89641 ccgtgttctc tcagtatacc cggcaaactt ttatcttctg cgctatttgc tggcaaaatc
    89701 gttgtgacca tgatgtatcc taatgccaat ttgtcttcaa tcaaaattcc gacactgata
    89761 cctactgcgt aacctaacgc atagacaaat aaattcagtg gattatctaa acgatctaaa
    89821 acaagggata agccgacgat ataaatcgtg atctcgacca tactgacaag tggagcaatc
    89881 agtcgatagc ctttcattgt caacatgaaa cgtaacgtgt tcagagtgat ataagcaaaa
    89941 ttaatgagga agatcatccc caacattttt ggatcaatca tttgtaaaac tcctttcatt
    90001 tatataacaa caatatcaaa aaaaatcgta agtgaagaaa aattgaaact ttatacatac
    90061 aaaaaaatga taaaatgatt ataatagaat tataaagata ggttaggtga ttaaaatgaa
    90121 aacggtcaat cgtgtgtttt gtgccagcca cgtgctaagt ctccttctca gtatatactt
    90181 aggtgtctat tcgattcaac agccaattga ggtagagaac attgtttttc agatgtcgct
    90241 gacagatggt ttaattttaa gcgttttttt ctttttgata ctgtttattg gtaatatttt
    90301 cggtagcgtt gccagttttt tgaatttttc tatttatcca ctcctttcgc tagcattagg
    90361 agtggtggga ctttgttctc tgtgcttatt tcccgagcca ttcagtcctg catttatatt
    90421 atttggtaca ctgaacctgt tccagacaac tgttggagca tggttgctat ggcgatcagg
    90481 aaatttaatg aaaataggtg agtaacaatt gaatccaatt atattgaaac gttctgtcca
    90541 tcatattcca gtgttagaag ttgttctgaa agaattaaga gaggaacgta ttcccgttgt
    90601 catctattat cacggatggc agacaagtaa ggagttagtt ttgacgcaag gaagaaaaat
    90661 tgcgcagcag ggcattcgtg tgatcctgcc agatgcgatg aatcatgggg agcgcaaaca
    90721 gccggtttct tctattccat cctatacatt ttgggatagt atctacggaa atctgtttga
    90781 atttgacaca cttatcaagc atttagaaaa aagaggtctt ctacatgatc agcttgcagt
    90841 tggcggggtt tcgatggggg gaatgacaac atgcgccttg ttggctaaac atcctcatat
    90901 agtagcgggc gtgtgtttga tgggttcccc tgcacctttg gaatatggaa aagaaatcgt
    90961 aaggcgcgca tcggaatata attatgtcgt accaaaagac tattttgatt tgatttcatg
    91021 ggttaagagc tatgatttgt cattgcagcc ggaaaagtta gctggacgtc ctctgttctt
    91081 ttggcatgga caagctgacg aaaaaatccc ttatatacaa acggaacgat ttgtagaaaa
    91141 aaatcggcat aagtcttacg gaaaagaaat tgtttttcgt tcctcaaaaa cacagcgaca
    91201 tatggtacaa ataccactaa tggaagaagc ttccactttt ttagcaaaaa atttgataaa
    91261 aaagtataaa taattgaaac ggaaaatcac tcatgacgat agaggatttt ccgtttttta
    91321 ttttgggcta taataaactc tgttaggaaa atttggtata attgatagta agaatgacaa
    91381 catgttagaa aggaagagag catgcccact ttagcagatg ttgcaaaacg tgccaatgtt
    91441 tcaaaaatga cggtctctcg agtcatcaat catccggaac aggtgactga cgaattgaaa
    91501 gagttagttt tctcagcaat ggctgaactg gattaccgtc caaatattgc tgcaaaagca
    91561 cttgtcacga atcggtcgca gatcatcaaa ttatttatac tggaagaaat cgacacaact
    91621 gagccctatt acatgaattt gctgatggga atcgctaaac ggatcggtaa agcgcattat
    91681 tctctccaat tagttacaaa tgatgccttc gacgtcggtt cttgtgatgg atacatcatc
    91741 acaggtgtca gaaaaaaaga cttcgattgg atcgaccgtt tggaaaaacc agttgtcttg
    91801 tttggggaaa attctgaagg atatgattat gtagacagcg ataatgaata tggtacagca
    91861 aaagcaacgc aatatgcctt agaaagagat tatcaaacga ttatttatat cggtatcgaa
    91921 gtggacgagc cttttgaaat ttcaagggaa actggttatt tatctgtgat gaaggaagtt
    91981 gatcttccta gtaaaatctt gcggtttcca aatcattcga cagaagtaga aagatatatg
    92041 acagaaaact gggcagattt tacagataaa acttgttttg tatgtagttc agatcggcta
    92101 gcactaggag cagtaagagg gattataaat gctggaggac acttgcccga agaatttggt
    92161 gtgattggat ttgatggggt atttttagac caaatcagtt cacctaaatt gacaacgatc
    92221 aagcaagata ttatacgtat gggtgaagta tgtggagaaa tgttgttaaa aaaaatagaa
    92281 gaaaaagggg cgagtcaagg ttatcgtcgt ttttttcctg aactagttat cagagagaca
    92341 acgaattaag tcttattact atatat
//
LOCUS       DACECY010000002        81266 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.002, whole genome shotgun sequence.
ACCESSION   DACECY010000002 DACECY010000000
VERSION     DACECY010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 81266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.002"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..411
                     /locus_tag="IWO38_000084"
     CDS             <1..411
                     /locus_tag="IWO38_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ0312207.1"
                     /translation="GLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAP
                     QEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLA
                     ELKGYQVTTGEPVCQTRRLNSRIDKVRYMFNKIT"
     gene            509..697
                     /locus_tag="IWO38_000085"
     CDS             509..697
                     /locus_tag="IWO38_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0312208.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(755..1108)
                     /locus_tag="IWO38_000086"
     CDS             complement(755..1108)
                     /locus_tag="IWO38_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0312209.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1639..2061
                     /locus_tag="IWO38_000087"
     CDS             1639..2061
                     /locus_tag="IWO38_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0312210.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2058..2288
                     /locus_tag="IWO38_000088"
     CDS             2058..2288
                     /locus_tag="IWO38_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0312211.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            2786..2986
                     /locus_tag="IWO38_000089"
     CDS             2786..2986
                     /locus_tag="IWO38_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0312212.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3013..4206
                     /locus_tag="IWO38_000090"
     CDS             3013..4206
                     /locus_tag="IWO38_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0312213.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="IWO38_000091"
     CDS             complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="IWO38_000091"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ0312214.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(5898..6821)
                     /locus_tag="IWO38_000092"
     CDS             complement(5898..6821)
                     /locus_tag="IWO38_000092"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ0312215.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7017..7622
                     /locus_tag="IWO38_000093"
     CDS             7017..7622
                     /locus_tag="IWO38_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0312216.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            7930..8946
                     /locus_tag="IWO38_000094"
     CDS             7930..8946
                     /locus_tag="IWO38_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0312217.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9087..10388
                     /locus_tag="IWO38_000095"
     CDS             9087..10388
                     /locus_tag="IWO38_000095"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ0312218.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            10434..11096
                     /gene="deoC"
                     /locus_tag="IWO38_000096"
     CDS             10434..11096
                     /gene="deoC"
                     /locus_tag="IWO38_000096"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ0312219.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11117..11515
                     /locus_tag="IWO38_000097"
     CDS             11117..11515
                     /locus_tag="IWO38_000097"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ0312220.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            11581..12663
                     /locus_tag="IWO38_000098"
     CDS             11581..12663
                     /locus_tag="IWO38_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0312221.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            12875..14437
                     /locus_tag="IWO38_000099"
     CDS             12875..14437
                     /locus_tag="IWO38_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0312222.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            14430..15563
                     /locus_tag="IWO38_000100"
     CDS             14430..15563
                     /locus_tag="IWO38_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0312223.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            15563..16519
                     /locus_tag="IWO38_000101"
     CDS             15563..16519
                     /locus_tag="IWO38_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0312224.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            16618..17784
                     /gene="deoB"
                     /locus_tag="IWO38_000102"
     CDS             16618..17784
                     /gene="deoB"
                     /locus_tag="IWO38_000102"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ0312225.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            17808..18626
                     /locus_tag="IWO38_000103"
     CDS             17808..18626
                     /locus_tag="IWO38_000103"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0312226.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            18643..19350
                     /gene="deoD"
                     /locus_tag="IWO38_000104"
     CDS             18643..19350
                     /gene="deoD"
                     /locus_tag="IWO38_000104"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0312227.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(19648..20334)
                     /gene="gpmA"
                     /locus_tag="IWO38_000105"
     CDS             complement(19648..20334)
                     /gene="gpmA"
                     /locus_tag="IWO38_000105"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ0312228.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(20506..21087)
                     /locus_tag="IWO38_000106"
     CDS             complement(20506..21087)
                     /locus_tag="IWO38_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0312229.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(21220..21903)
                     /gene="rpiA"
                     /locus_tag="IWO38_000107"
     CDS             complement(21220..21903)
                     /gene="rpiA"
                     /locus_tag="IWO38_000107"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ0312230.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            22222..22635
                     /gene="rpsL"
                     /locus_tag="IWO38_000108"
     CDS             22222..22635
                     /gene="rpsL"
                     /locus_tag="IWO38_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ0312231.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            22719..23189
                     /gene="rpsG"
                     /locus_tag="IWO38_000109"
     CDS             22719..23189
                     /gene="rpsG"
                     /locus_tag="IWO38_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ0312232.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            23283..25370
                     /gene="fusA"
                     /locus_tag="IWO38_000110"
     CDS             23283..25370
                     /gene="fusA"
                     /locus_tag="IWO38_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ0312233.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            25542..26729
                     /gene="tuf"
                     /locus_tag="IWO38_000111"
     CDS             25542..26729
                     /gene="tuf"
                     /locus_tag="IWO38_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ0312234.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            27136..27444
                     /gene="rpsJ"
                     /locus_tag="IWO38_000112"
     CDS             27136..27444
                     /gene="rpsJ"
                     /locus_tag="IWO38_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ0312235.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            27475..28104
                     /gene="rplC"
                     /locus_tag="IWO38_000113"
     CDS             27475..28104
                     /gene="rplC"
                     /locus_tag="IWO38_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ0312236.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            28132..28755
                     /gene="rplD"
                     /locus_tag="IWO38_000114"
     CDS             28132..28755
                     /gene="rplD"
                     /locus_tag="IWO38_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ0312237.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            28755..29045
                     /gene="rplW"
                     /locus_tag="IWO38_000115"
     CDS             28755..29045
                     /gene="rplW"
                     /locus_tag="IWO38_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ0312238.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            29084..29917
                     /gene="rplB"
                     /locus_tag="IWO38_000116"
     CDS             29084..29917
                     /gene="rplB"
                     /locus_tag="IWO38_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ0312239.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            29960..30238
                     /gene="rpsS"
                     /locus_tag="IWO38_000117"
     CDS             29960..30238
                     /gene="rpsS"
                     /locus_tag="IWO38_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ0312240.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            30261..30608
                     /gene="rplV"
                     /locus_tag="IWO38_000118"
     CDS             30261..30608
                     /gene="rplV"
                     /locus_tag="IWO38_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ0312241.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            30622..31278
                     /gene="rpsC"
                     /locus_tag="IWO38_000119"
     CDS             30622..31278
                     /gene="rpsC"
                     /locus_tag="IWO38_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ0312242.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            31281..31715
                     /gene="rplP"
                     /locus_tag="IWO38_000120"
     CDS             31281..31715
                     /gene="rplP"
                     /locus_tag="IWO38_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ0312243.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            31705..31893
                     /gene="rpmC"
                     /locus_tag="IWO38_000121"
     CDS             31705..31893
                     /gene="rpmC"
                     /locus_tag="IWO38_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ0312244.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            31918..32184
                     /gene="rpsQ"
                     /locus_tag="IWO38_000122"
     CDS             31918..32184
                     /gene="rpsQ"
                     /locus_tag="IWO38_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ0312245.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            32242..32610
                     /gene="rplN"
                     /locus_tag="IWO38_000123"
     CDS             32242..32610
                     /gene="rplN"
                     /locus_tag="IWO38_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ0312246.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            32647..32955
                     /gene="rplX"
                     /locus_tag="IWO38_000124"
     CDS             32647..32955
                     /gene="rplX"
                     /locus_tag="IWO38_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ0312247.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            32983..33522
                     /gene="rplE"
                     /locus_tag="IWO38_000125"
     CDS             32983..33522
                     /gene="rplE"
                     /locus_tag="IWO38_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ0312248.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            33541..33726
                     /locus_tag="IWO38_000126"
     CDS             33541..33726
                     /locus_tag="IWO38_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ0312249.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            33762..34160
                     /gene="rpsH"
                     /locus_tag="IWO38_000127"
     CDS             33762..34160
                     /gene="rpsH"
                     /locus_tag="IWO38_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ0312250.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            34192..34728
                     /gene="rplF"
                     /locus_tag="IWO38_000128"
     CDS             34192..34728
                     /gene="rplF"
                     /locus_tag="IWO38_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ0312251.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            34889..35245
                     /gene="rplR"
                     /locus_tag="IWO38_000129"
     CDS             34889..35245
                     /gene="rplR"
                     /locus_tag="IWO38_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ0312252.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            35266..35766
                     /gene="rpsE"
                     /locus_tag="IWO38_000130"
     CDS             35266..35766
                     /gene="rpsE"
                     /locus_tag="IWO38_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ0312253.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            35781..35960
                     /gene="rpmD"
                     /locus_tag="IWO38_000131"
     CDS             35781..35960
                     /gene="rpmD"
                     /locus_tag="IWO38_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ0312254.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            36004..36444
                     /gene="rplO"
                     /locus_tag="IWO38_000132"
     CDS             36004..36444
                     /gene="rplO"
                     /locus_tag="IWO38_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ0312255.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            36444..37739
                     /gene="secY"
                     /locus_tag="IWO38_000133"
     CDS             36444..37739
                     /gene="secY"
                     /locus_tag="IWO38_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ0312256.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            37801..38448
                     /locus_tag="IWO38_000134"
     CDS             37801..38448
                     /locus_tag="IWO38_000134"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ0312257.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            38639..38857
                     /gene="infA"
                     /locus_tag="IWO38_000135"
     CDS             38639..38857
                     /gene="infA"
                     /locus_tag="IWO38_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ0312258.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            38890..39006
                     /gene="rpmJ"
                     /locus_tag="IWO38_000136"
     CDS             38890..39006
                     /gene="rpmJ"
                     /locus_tag="IWO38_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ0312259.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            39024..39389
                     /gene="rpsM"
                     /locus_tag="IWO38_000137"
     CDS             39024..39389
                     /gene="rpsM"
                     /locus_tag="IWO38_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ0312260.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            39417..39806
                     /gene="rpsK"
                     /locus_tag="IWO38_000138"
     CDS             39417..39806
                     /gene="rpsK"
                     /locus_tag="IWO38_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ0312261.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            39886..40824
                     /locus_tag="IWO38_000139"
     CDS             39886..40824
                     /locus_tag="IWO38_000139"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ0312262.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            40852..41232
                     /gene="rplQ"
                     /locus_tag="IWO38_000140"
     CDS             40852..41232
                     /gene="rplQ"
                     /locus_tag="IWO38_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ0312263.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            41504..42343
                     /locus_tag="IWO38_000141"
     CDS             41504..42343
                     /locus_tag="IWO38_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0312264.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            42319..43188
                     /locus_tag="IWO38_000142"
     CDS             42319..43188
                     /locus_tag="IWO38_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0312265.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            43178..43975
                     /locus_tag="IWO38_000143"
     CDS             43178..43975
                     /locus_tag="IWO38_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ0312266.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            44178..44924
                     /gene="truA"
                     /locus_tag="IWO38_000144"
     CDS             44178..44924
                     /gene="truA"
                     /locus_tag="IWO38_000144"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ0312267.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(45257..47926)
                     /locus_tag="IWO38_000145"
     CDS             complement(45257..47926)
                     /locus_tag="IWO38_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ0312268.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(48164..48352)
                     /locus_tag="IWO38_000146"
     CDS             complement(48164..48352)
                     /locus_tag="IWO38_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ0312269.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            48622..48984
                     /locus_tag="IWO38_000147"
     CDS             48622..48984
                     /locus_tag="IWO38_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0312270.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            49036..50718
                     /locus_tag="IWO38_000148"
     CDS             49036..50718
                     /locus_tag="IWO38_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ0312271.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            50835..52871
                     /gene="recG"
                     /locus_tag="IWO38_000149"
     CDS             50835..52871
                     /gene="recG"
                     /locus_tag="IWO38_000149"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ0312272.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            52920..53921
                     /gene="plsX"
                     /locus_tag="IWO38_000150"
     CDS             52920..53921
                     /gene="plsX"
                     /locus_tag="IWO38_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ0312273.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            54043..54285
                     /gene="acpP"
                     /locus_tag="IWO38_000151"
     CDS             54043..54285
                     /gene="acpP"
                     /locus_tag="IWO38_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ0312274.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            54553..55557
                     /locus_tag="IWO38_000152"
     CDS             54553..55557
                     /locus_tag="IWO38_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0312275.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            55558..56502
                     /locus_tag="IWO38_000153"
     CDS             55558..56502
                     /locus_tag="IWO38_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0312276.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            56502..57464
                     /locus_tag="IWO38_000154"
     CDS             56502..57464
                     /locus_tag="IWO38_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0312277.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            57491..58405
                     /locus_tag="IWO38_000155"
     CDS             57491..58405
                     /locus_tag="IWO38_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0312278.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            58431..60212
                     /locus_tag="IWO38_000156"
     CDS             58431..60212
                     /locus_tag="IWO38_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0312279.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            60560..61246
                     /locus_tag="IWO38_000157"
     CDS             60560..61246
                     /locus_tag="IWO38_000157"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ0312280.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            61266..64847
                     /gene="smc"
                     /locus_tag="IWO38_000158"
     CDS             61266..64847
                     /gene="smc"
                     /locus_tag="IWO38_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ0312281.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            64856..65665
                     /locus_tag="IWO38_000159"
     CDS             64856..65665
                     /locus_tag="IWO38_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0312282.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            65676..66674
                     /gene="ftsY"
                     /locus_tag="IWO38_000160"
     CDS             65676..66674
                     /gene="ftsY"
                     /locus_tag="IWO38_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ0312283.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            66861..69128
                     /gene="gshAB"
                     /locus_tag="IWO38_000161"
     CDS             66861..69128
                     /gene="gshAB"
                     /locus_tag="IWO38_000161"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ0312284.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(69217..69669)
                     /locus_tag="IWO38_000162"
     CDS             complement(69217..69669)
                     /locus_tag="IWO38_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ0312285.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            69819..70769
                     /locus_tag="IWO38_000163"
     CDS             69819..70769
                     /locus_tag="IWO38_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ0312286.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            70762..71721
                     /locus_tag="IWO38_000164"
     CDS             70762..71721
                     /locus_tag="IWO38_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ0312287.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            71718..72473
                     /locus_tag="IWO38_000165"
     CDS             71718..72473
                     /locus_tag="IWO38_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0312288.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            72496..73449
                     /locus_tag="IWO38_000166"
     CDS             72496..73449
                     /locus_tag="IWO38_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0312289.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(73972..74268)
                     /locus_tag="IWO38_000167"
     CDS             complement(73972..74268)
                     /locus_tag="IWO38_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ0312290.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            74623..74979
                     /locus_tag="IWO38_000168"
     CDS             74623..74979
                     /locus_tag="IWO38_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0312291.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            74989..75888
                     /locus_tag="IWO38_000169"
     CDS             74989..75888
                     /locus_tag="IWO38_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAQ0312292.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            76405..77136
                     /locus_tag="IWO38_000170"
     CDS             76405..77136
                     /locus_tag="IWO38_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0312293.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            77281..79308
                     /locus_tag="IWO38_000171"
     CDS             77281..79308
                     /locus_tag="IWO38_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAQ0312294.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            79416..80513
                     /locus_tag="IWO38_000172"
     CDS             79416..80513
                     /locus_tag="IWO38_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ0312295.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            80820..>81266
                     /locus_tag="IWO38_000173"
     CDS             80820..>81266
                     /locus_tag="IWO38_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0312296.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT        26641 a        14563 c        17368 g        22694 t
ORIGIN      
        1 ggtttatact atagccctgt tagtactcca gcagattttc ggatgcttac tcctattgta
       61 ctggagcaag cctttagaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 gtttatgcac cacaggaata tctttcacgg gcatataacg atgctcccaa atattgtgca
      181 aatatcgtaa atactcaact gaaaaataat gaggtcatta ttattggaga aattcctgct
      241 cgatgtattc aagattatcg caatgattta acttttttta caaatgggct tagtgtttgt
      301 ttagcagagc taaaaggata tcaggttacc actggcgaac ctgtttgcca gacccgtcgt
      361 ctaaatagtc ggatagataa agtaagatat atgttcaata aaataactta gtgcgtttta
      421 tgttgttata taaatatggt ttcttattaa ataagatgaa atattcttta atatagattt
      481 gaattaaagt ggaaaggagg agattgttat tataaactac aagtggatat tgtgtcctat
      541 ttgtggaaat aaaacaagac tacgaatacg agtggatact atacttaaaa atttcccttt
      601 atactgcccc aaatgtaaga acgaaacttt aattaatgtt caaaaaatga atataataac
      661 aatcaaagag ccagacgcca agacgcagag ccgataattt gagaaatgaa actctcatct
      721 tatcggctct ttttgtttat ctgaatttta ctgactagcc ttcaatattt cttgcttcat
      781 tgataccttt tgctagtgct tccattaagg atagttcttt gtctgtaaag ctatccatgt
      841 atttttctac ctgtaacctt cgagtgcttt taatcaaatt atctgtaggt aagaaaaatt
      901 catcaataga tacatggagt aatgatacaa gatcatacag cacttgtgta cttgggtgtt
      961 gtcctttgtt ttctatattt gttaaataac gtgggtcaat ttcaatcatt gttcccactt
     1021 gttcacgagt gagaccccgt ttcattcgag cctctttaat cgctaaacca acggctctaa
     1081 aatcgtattt atcttctttt ttacgcatac taaatcacct ctatacattt tactgttcct
     1141 atataaccat aaacaggtat agaaaaacat gttatatagt ttatgagttc ctatagaaca
     1201 ctgtggaatt tattaatgcg atgaatccaa tttcaaatag aaaggtcgct gattttattt
     1261 acctatacaa ctttataaaa tttagtcata ctcctagaaa gtagataaaa ttggcgactt
     1321 aatggaggga aaagtcgcca tgagaaagat aatctgtcat ctagttctct taattaagtt
     1381 cttatcaaaa aaacttatcc acctgtactg gatagatatg accatcaatg tggttttgca
     1441 gattacatag atagaaaaat caaagcatat gaacagagaa aacaatctgt ttatgtgctt
     1501 ttttggtcac ttaataaata tcttttagga tttctcttca tttatgcaac aaatcccccg
     1561 ttcgtgtcgg ggtttagtat gaagggagat aaagaagcac atgccactcc ctttcacgac
     1621 agaaaggggg tgaaaagcat gaaaccatct tcttttcaag aaaccataga aaatcagttt
     1681 gactatatct gcaaacaagt tattgaagat gaacgaaaag attacttcaa acatctagac
     1741 agactatcag caaaagaagt ctcgttttct gatatagata actaccttgt caactctttt
     1801 tctactgttg atacgtacgt cactgatttc caactattta aattatttgg attaagaatc
     1861 aatgttgaaa gtgatttatt aagtgaagcc ctgcgtcatc tttcagagaa aaaacggaac
     1921 attattttac ttcattactt tatggatatg agtgatgtgg agattgcgga gttactaaat
     1981 ttgaatcgtt cgactgttta tcgtcatcga ataagtggac tagccatgat taaagaattc
     2041 atgaaggagt gtaaagaatg aaaacagctt atccaagggt tcccttcccc ctcattgtaa
     2101 aggcgacaga tggcgacgtg gaagcaatta atcagattgt taaacattat cgagggtata
     2161 cctcaaaacg ttcattacgt cgcatgacag acgaatatgg aaattctcat atggttattg
     2221 atgaaaccct acgtggcaga atggaaacga aactcatctc taagatttta tcgttcgaaa
     2281 ttaaatagtc ccattttttc gtggaagcgt gtcgaatcgt atccacgctt cccgatttgg
     2341 gaggtttgtt attccatcaa agcatattgt gtgttcagta ggttttgata ggctaacaag
     2401 ccaaacgttc tttgacaacc aaataaaggc aatcgaatac gtttcggtaa gaaaagagct
     2461 gatgaactaa cacgccatga cctacaaaat tagtgagcga ttcatgttag taattcgaaa
     2521 gtaatcttta gcaggattgc tgccatgact ttcttatcgt gataatgata ctcccgtata
     2581 gccatagtcc gagcgttaga agcgtcacag gcaatgggta cggctacatg agaaccatgc
     2641 agaagtgaaa ttcctgtgag ctttgctaga gctgttcgat tgctaagata ttcttctctt
     2701 gtcagtgtat tattataaaa ctatttgata taattatagg tgaatattgg agttttccta
     2761 gcctataatt gggaaaggag taataatgat aacggtcaca aaaaaggatt tagttgagct
     2821 aggatatgga acttcttttt cagcagacat cataaaaaaa gcaaaagaat tgatgattac
     2881 aaaagggcat atctactatc aatctcgaaa gctagataga gtccctcgtg aagctgttga
     2941 agaaattcta ggaattcggt tttcaaatga aaacaggtct gagtagtttt tgcacagtaa
     3001 ggagtaaaaa ctatggttaa agacccaata aaaaaagcaa agaatggaac ctattatttt
     3061 cgtgcaaatc taggttatga ttctaaaggg aaaaaaattc aaaaatatcg tagtggattc
     3121 actactaaaa aagaagcaag agaagaatat tcgaaactgc tattgatgaa accagaagaa
     3181 ttaagtgaga ataaggataa aatgacgttt aaacattata tctttgaaat tttccttcca
     3241 tggtataaga cacaggtaaa gctcagaaca tatgaaaatc gcttaccaac tattaagaag
     3301 catttctctt attttgataa aatggctgtt tccgatattg agcctattga tgtgcaaaat
     3361 tggcaactaa aattatcaaa gaagtgtaag tcttcttatg taagagctgt tcaaggacta
     3421 ttttcagttg ctatggatcg agcaattgtc cttgggataa ccaccacaaa tccatctaag
     3481 attattggca atgtgaaaaa gcaaaagtct aaaattgaat tttggacaaa agaagaattt
     3541 gaaaaagtaa tctctctaat ttacaaagaa gattactatc aacacttttt atttatctct
     3601 ctgtggtttt tatttatgac tggaatgcga attggtgaag ctactgccat tcaatgggaa
     3661 gatattgatt ttgactcagg agtactaacc atcaacaaaa cactctatta caagaatcaa
     3721 tcgaattata gatttgttga accaaaaaca aaggcgagcg ttcgtcatat tgtattagat
     3781 aaatgcacat taacttattt atccgaatgg aaggaagtac aacaaaatct aattcagaca
     3841 gactttgtaa tgagctataa cggtattcct acccaaaaac atacactatc atatgctatt
     3901 gaacgctatg ctaagctggc tggtatccat cgaatcagaa tccatgggtt acggcattct
     3961 catgcttcct tgttaatcag tatgggagaa aatccactta tcattaaaga tcgtctagga
     4021 cacgaagata ttgagacgac tttaggaacg tatggacatc tttatcctaa cagtaatttc
     4081 gaggtagcta acaaattaaa tggagccatt tcctttaaag aagcaaaaca aaattatgat
     4141 acttcaccaa agaatcaatt tactgtagac tacttgcgaa acaaagaaat gaaaaaagtg
     4201 caataaaagt gcagtaaaaa gataagaaga gccagaaacc cttggtataa gaggatttct
     4261 agctctccta caactattcc cactccactg ttgctggtgg tttagaagtg atgtcataca
     4321 cgatacggtt gacgtgggct acttcgttta cgatacgtac agagattttt tgcaagactt
     4381 cccatgggat acgggcaaag tcagcggtca ttccgtcgat tgaagttaca gcacggatac
     4441 cgactgtgta gtcgtaagta cgtccgtcac ccataacacc gacagagcgg atccctggaa
     4501 gaacagtgaa atattgccag atatcacggt caagtccagc agctgcgatt tcttcacgca
     4561 agatagcatc agattcgcgt acgatttcta gcttgtcttc agtgatttcg ccaagaacac
     4621 gaataccaag accaggacca gggaatggct gacgccaaac gattgaatca ggcatgccta
     4681 actgagtccc taattcacgg acttcgtctt tgaataatgt gtttaatggt tcgatcaatt
     4741 caaattgcat atcttctggc agtccaccta cattatggtg agatttgatc gtctgtgctg
     4801 tttctgtacc agattcgata acgtcagtat acaacgttcc ttgtgctagg aaagagacgc
     4861 cttcttcacc agcaagtttt gttgcttcgt catcaaaaac atatacgaat tcattcccaa
     4921 tgattttacg tttttgttct gggtcagata cacctttcaa cttgtctaag aagcgatctc
     4981 ttgcatcgac tttgatgatg ttcaagccga attttccgcc tagactttcc attacttgtt
     5041 ccgcttcgcc tttgcgtagc agaccgtggt caacaaagat agatgtcaat tggtcaccga
     5101 tcgctttttg taaaagaacc ccaacgacac tagaatcaac gccacctgaa agaccaagca
     5161 agactttttt gtcaccaact tgttcacgga ttttttctac ttgcatatcg ataaagttat
     5221 ccattgtcca gttaccttca cagtgacaaa catcaaaggc aaagtggcgc agcaattcga
     5281 ttccgtattc ggaatgacgt acttctgcat ggaattggat accataaaaa ttacgtgttg
     5341 tgtcttggat agaagcaatc ggacaatctt tgcttgttcc gactgtttca aagccttcag
     5401 gaactttagt taccaagtca ccatggctca tccatactgt ttgttgtttt ggtgtttctt
     5461 taaacaagac agcttgatcg cttgttactt ctaacaaggc tttcccatat tcgcggtttt
     5521 tagcaggctc tacggaaccg ccaagtttgt acgtgatcaa ttgcatacca tagcaaatcc
     5581 ctaatacagg aatacctaaa ttgaagatct caggatcgat cccaaatgca ttctcatcat
     5641 atacactgtt tggtccgcca gaaagaatga ttccctttgg attcatcgct tttacttctt
     5701 ctgcagtaat gcggtggctc aataattcgg aaaagactcc aagatcacga atacgtcttg
     5761 tgatcaattg gttatactga ctaccgtagt ctaatacgat aatcttttca acgttcgtca
     5821 tatcggcaac gtttgtcaaa tttattaccc ctatcccttt aaattttatc tcaaaatagt
     5881 caaactattt taaaatgcta atattttcgc atgaaaatcg aatcgatgat atgattttca
     5941 gttttatgga gaattatatc ggctcttcct cgtgtaggca agatatattc ctgtaaattt
     6001 ttcaaattca catttttcca tacatctttt gccattttaa aggcttcttc acgatctccg
     6061 atagcaaaac gataataata attctctgga ttttggaaag ccgtatccag taacgcacca
     6121 aaacgatcca aataccattt ttcgatcagc acgggatcgg catcgacata gacagaaaaa
     6181 tcaaaaaagt cgctgacgta gatttgctga ttcgcaggta attgaagcgt attgatcccc
     6241 tcaacgatca agatatcagg ctgctggatc aattcgtact tgtctggaat gatatcatac
     6301 acatcatgtg agtaaacagg tacttttatc tcttcctgac cagattttac acggttcaag
     6361 aaatcgatca gcatctccat atcataactt tcaggaaatc ctttacggtc catgattcca
     6421 cgctctttta atatacgatt tggataaaga aatccgtcag tcgtgatcag ctgaacgttt
     6481 cttcgtttga aggtacgaga caaaatcgtc tgcagcagtc tggctgttgt actttttcca
     6541 acggctacac ttccggcgat cccgataata aaaggcggta cttctacgta tttatgaaga
     6601 aataaacctt tactcaagaa caaagattca aattctttca tataaagata aatcagatga
     6661 gcaagaggta catagatatc tcgaacatcc tgcatggaaa tgcggtcatt caaacttttg
     6721 atattatcta gttcatcttg tgtcaaaggt gcttgcccat tacgataaaa ttctcgccat
     6781 tcttctcttg ccacacggta gtagttcatt ggatcattca tattagcctc ctgcgttctc
     6841 ccatcaatgg aactatttta acacaaaata aacgcaaacc ccaccactta agagagattg
     6901 ccgatctttt tgtataatct ttataataga aagaaacgga gtcttattct tgacttgcgc
     6961 acggatacat aaaaggatag actgttgtca tgaaagaaat gagaaggatg gaagacatga
     7021 caaatcatta ctattctgaa cgcccagata cggcgcacga ttttgaacaa tgggcttttg
     7081 agttaaaagg cagaaccttt caatttgtaa cagattctgg tgtattttca cgtgaaacag
     7141 tggattatgg ttcacgtgtc ctgattgatg ctttcgattg ggaagagtta cctgatgaag
     7201 gaaagatctt agatgtggga tgcgggtatg gaccgattgg gttagctttg gcatttgcga
     7261 cacagcggtt tgttgaaatg gtcgacatta atgaacgagc ggtaagtcta gcgcaaggaa
     7321 acgcaaagcg taaccaaatc gaacatgttg atatccatca atcgaatatc tatgaagcag
     7381 ttcatgaaga aacctatgca gctatcgtaa gcaatccgcc gatccgtgct ggaaaaaaag
     7441 ttgtacatga aattttgaca ggcgcatatc ctcggttgaa gaagggcggt acactgacaa
     7501 ttgtgatcca gaagaaacaa ggagcgccga gtgcgcaaaa gaaaatggaa gaaacatttg
     7561 gtaatgcaga aatcgtcaca aaagataaag gatattatat catcaaaagt gtgaaagaat
     7621 aactagctga aaaatagtgg agcgacgaaa gagtggaact gggcgtgcat gtacgggtac
     7681 gtatcagttc tttttttgtt gagaagtttt taaaaacaaa gaactatttt ttcttcaaaa
     7741 agaaatagta ggaacagagt cgttgttttt tacgataaaa taaataagat acagaacttg
     7801 tatagaaggc tgccaagtcg gtagataaag gaggttttta tcaaaaaaca tactttcctt
     7861 ttttctctat tcttttcttg ggggttttgg tacactgata gggaatttag atagaaggga
     7921 gccaatagga tgaagctgac catcagagat atcgcagaaa tggcaggcgt atcagtaaca
     7981 actgtctcac agattttgaa taacaaagga agcagattta gtgataagac aagaaaaaaa
     8041 gtgttgtcga tcgtgaatga acatcattat aagcccgatt atttcgcttc aaatgtgatc
     8101 aatcgacatt caaagaccat cggaatgatc gtccctgatg tcactgattt cttcttctcc
     8161 aaactgatcg aaggtgtaga gagctatttg aatcctttgg gctatgtgat catgttatgc
     8221 aattctcgtc atagccaaga aaatgagatc aagtatttac aagagctttc ccaccgctca
     8281 gtggacggaa tcttgctggc cacaccaaat attttacctg agaaatatgc attagacagc
     8341 ggtttttatc aaaaaatgcc aatcatttta atcgatcgcg gattgaatcg tcgggataac
     8401 ggacggctta tcgtaaagga atatgagggc gcctatcaag ctgtttcgct gtttattgaa
     8461 aatggccata cgaagatcgg catgctgaag gaaaccactg gatactatca attagaagaa
     8521 cgtttcaacg gatatcgtca tgcgctgaaa gatcatggcc tgccttttaa tgggaaattt
     8581 gttgaacatg gagagttgac tgtccaaggt ggatatactg catcaaaaaa attactcaaa
     8641 cataaagata tcacggctat tttttgcggg aatgatgcca tggcgatcgg atgctatcag
     8701 gcgatcgatg aattgggcaa aaaaatccca gaagatatct cagtcatagg attcgatgga
     8761 ttaaagctgt cagaatacat gattccgcaa ttgacgacag tgcagcagcc aagttttgat
     8821 attggatttt atgctgcacg tttcctgatc gatacgatcg aatttcctca aagaaaagtt
     8881 ccgaacaaag tgttcgaaac aaaattaatt attcgtgaga gcgtaaaagt attgacaaag
     8941 ggctaactcg gcggtatatt gcttatgtaa gcatttacaa aggctagtta gtgctttttt
     9001 tatttttagt aaaccagttt actaaagtgg tttacctttc ctaaaaataa tgtataatac
     9061 cgaatgaaga caaggaggat caggcaatgc gaatggtgga tctgattgaa aaaaagaggg
     9121 atggaaacga gctgtcgaaa gaggaaatcg agtacatcgt cacgaactat acgaatggaa
     9181 agattccaga ttatcaagtc agtgcattat tgatggcaat tttttatcaa gacatgacaa
     9241 atgaagaaat cacgaactta acattggcga ttgctaattc tggtgatgtc atcgatttga
     9301 gttctcttga aggaataaag gtagataagc attcgacagg tggcgttggt gacacgacca
     9361 cattgatttt agccccccta gtagctagtg tcggagtgac agtggctaaa atgtctggac
     9421 gcggtctagg ttacacaggt ggtacactag acaagctaga agcaattccc ggatttcaga
     9481 tcgagctttc ggatgaagaa tttgtacgca ttgttaatga aagtaaagta gcggtaattg
     9541 gtcaatcagg aaacttggca ccggcggaca aaaaattgta tgccttgcgt gacgtcacag
     9601 cgaccgttga ttctcttccg ctgattgcca gttcgattat gagtaaaaaa atcgcggcag
     9661 gagcagatgc aatcgttttg gatgtgacca ctggagacgg tgctttcatg aagaacatcg
     9721 aagatgcaag acgtctggca aagaccatga cgagcattgg gaaactggct aaccgagaaa
     9781 cagtggcggt tatctcagat atgtcagaac ctcttggcga agctatcgga aatagtctag
     9841 aagttgtcga ggcaatcgaa acattacaag gaaatggtcc agaagatcta gtagaaatgt
     9901 gctacgcctt aggaagccaa atggttgttc ttgctggcaa agctaagaca accgatgaag
     9961 caagaacgtt gcttcaagaa gctctggaat caggtaaagc gttagcaaaa ttcaaagaaa
    10021 tgatccaaaa tcaaggggga gacccagcga tcgtggaaca tccagaacga attttgactg
    10081 cacgctacac aatggagctt cctgctaaac aaagcggagt tgtgtcaaaa atcgtggcaa
    10141 atgaattagg aattgctgca atgatgctag gtgctgggcg taaaaccaaa gaagaagata
    10201 ttgatcacgc tgttggattg aaactgcata aaaaaattgg tgacactgtg acaaaaggtg
    10261 agtcactatt gacgatctat agtaacgacg aagaaatcac ttctgtcatt gaattattat
    10321 ataaaaatat tgaaattggt gaatcggcaa tgaagccaac gttgattcat gacatcatca
    10381 cagaataaag gaaaaaagcg gactttaccg ttttaaagaa aaggggaaaa gaaatggaac
    10441 taaaccgaat gatcgaccat actattttga aagcagacgc atctaaagaa gctgtgatgc
    10501 aaattatcga agaagcaaaa aaataccatt tttattctgt ctgcatcaat ccagcatggg
    10561 ttgcgctagc gaaagaacaa ttacaaggtg agccagtagc cgtatgtaca gtaatcgggt
    10621 ttcctctagg agcaaacaca cccgagacaa aagcatttga aacaacagat gccatcaata
    10681 acggtgcaga tgaagtcgat atggtcatca atatcggtgc tttgaaatca aaggatgatc
    10741 aacaagtcca aaaagatatc gaagcagttg ttgaagcagc aaaagatcgt gcgcttgtca
    10801 aagttattat tgaaacatcg ttattagata gagaagaaat catccgagct tgcgaaatag
    10861 caaaagctgc aggcgctgat tttgtcaaaa catcaactgg tttctcaaca ggcggagcaa
    10921 aggtagagga tgtccgtttg atgcgtgaaa ctgtaggacc agaaatgggt gtcaaagctt
    10981 ccggcggtat ccataacgaa gaagaagcaa tggcaatgat cgaagctggt gctacaagaa
    11041 tcggaacaag cgcaggagta gcaatcgtat ctggttcaac tggtgaaggc tattaatttt
    11101 ttcaaaggag aagctcatgg cagaactcaa acaagaatgg atcaacacag ctattgaagc
    11161 gttagataaa gcctatgttc cttattccca ttttccggta ggcgcttgtc tcgtaacgga
    11221 atcaggcaag atttatcaag gcatcaacat tgagaatgca tcatttggat taacgaattg
    11281 tgccgagcgc acagcttttt tcaaagcagt atctgaagga gaaaaaacat ttacgcatct
    11341 agtcgtagcg gggcacaccc ccgatcctat ctctccttgt ggtgcatgtc gacaagtaat
    11401 ggcagaattc tgttcccctg atatgccggt tacgctagta ggagacaatg gtgtgacgaa
    11461 ggcaacgacc gttcgtgcac tgctgcctta tgcatttact gaaaaagatc tataactcat
    11521 gttcattcag tcagctgact gttgaaaaat aaaacaatta ttttaggagg ctttacagaa
    11581 atgaaaaaag caaaattatt tggtttaggt gctgtcgcac tagcagcagg gttattttta
    11641 ggtgcatgcg gcaacaacgg ctctactgat tcatccggtg gcaaaagctc atcagacaca
    11701 acaactgcag cattgatcac agacacaggt ggtgtagacg accgttcatt caaccagtca
    11761 gcttgggaag gactagaaaa atgggggaaa gatcaaggac tttcacgagg aaatgatggc
    11821 ttccaatatt tccaatcttc aaatgaatca gattacatcc caaatattga ccaagcattg
    11881 aatgctggat tcaaaacgat ttttggtatt ggttataaac taaaaccagc gatccaagaa
    11941 caagcaacaa acaatacagg tacgaacttt gttattatcg atgatgtcat cgatggatta
    12001 gataacgttg tatctgcaac tttcaaagac aacgaagctt cttatcttgc aggtgttgca
    12061 gcagcttata cgactgaaac aaacgtagtc ggctttatcg gcggtgtaaa aggtgaagta
    12121 atcgatcgtt ttgatgctgg attcaaagcc ggtgttgatg caggtgcgaa agaattagga
    12181 aaagaaatca aagtattgaa tcaatatgct ggtgacttct ctgctccaga taaaggacgt
    12241 tcaattgctc aaggtatgta tgcacaaaac gcagatatca tcttccatgc ttcaggcggt
    12301 acaggtaacg gtgtattcca agaagcaaaa tcattgaacg aatcaggcga caaaaaagtt
    12361 tgggttatcg gtgttgaccg cgaccaatca gacgaaggcg aatacacatt aaacggcgaa
    12421 aagaaaaact tcacattgac atctacactg aaagcagttg gaacagtagt agaagatttg
    12481 gcacaaaaat cagcggacgg taaattccct ggcggcgaac acactgttta tggattgaaa
    12541 gaagacggtg ttggtctaac agaaggacaa ctttctgacg aagcgaaaaa agcagttgat
    12601 gaagcaaaag aaaaaatcat ttcaggtgac gtaaaagttc cagaaactcc tgaagaaaac
    12661 taaaaccatt tctatagcct tgttagacac atgtttgaca aggctatggt ttgattagaa
    12721 caagaattgt tcatttatcc aagctgcctt gtgacagttt ggataaggga acaactccct
    12781 gaagtaatgt ttgtttcaaa agaagaacgc tcactaagcg tgaatcttag tcaatcgttg
    12841 ccctagatag aaatcgttag gatgtgaaaa aaaggtggtt caagaaaata ttgtcataga
    12901 aatgcagaat attaccaaag aatttggaac attcaaggca aatgacaaca tcaatttaca
    12961 agtaaaggca ggagaaattc atgcgttgct tggagaaaac ggtgctggca aatctacatt
    13021 gatgaacgtg ctttccggat tattagagcc gacatcaggg aaaattttga tgcgtgggaa
    13081 agaagtaaag atcacaagtc cgaccaaagc caaccaatta gggattggga tggtccatca
    13141 gcactttatg cttgttgatg cctttactgt aacagaaaac atcgtgttgg gaagcgagcc
    13201 tagtcgcgca gggatgcttg accataaaaa agcacgaaaa gagatccaaa aagtttctga
    13261 acagtatgga ttgtcagtca acccagatgc ttacgttcgt gatatttcag ttggaatgga
    13321 acaacgggta gaaattttaa aaacacttta ccgaggagca gatgtactga tttttgatga
    13381 gccgacagct gtattgaccc ctcaagaaat tgatgaatta atcgtgatca tgaaggaatt
    13441 agtcaaagaa ggcaagtcga tcattttgat tacgcataag ttagatgaaa tcaaagcagt
    13501 agctgaccgc tgtacagtta tccgccgtgg aaaaggaatc ggtacagtca acgttaaaga
    13561 cgtaacctca caacaattag ccgatatgat ggttggaaga acggtttcat tcaaaacgat
    13621 gaaaaaagaa gcgaagcctc aagaagtcgt tttgtctatt gaaaatctag tggtgaaaga
    13681 aaatcgtgga ttagaagccg tgaaaaactt gaacttagag gttcgtgctg gcgaagtact
    13741 tggtatcgct ggaatcgatg gaaacgggca gtcggagttg atccaagctt tgactggttt
    13801 gcgaaaggca gaaagcggac atatcaagct aaaaggggaa gacatcacta ataaaaaacc
    13861 ccgaaaaatc actgaacatg gtgtaggaca cgtgccagaa gaccgtcata aatacgggtt
    13921 agtcctagat atgacattat ctgaaaatat cgcactacaa acctatcatc aaaaacctta
    13981 cagtaaaaac ggcatgctga attattcagt aatgaatgaa catgccagag aattgatcga
    14041 agaatatgat gttcgaacaa cgaatgaact tgttcccgca aaagctttat caggcggaaa
    14101 tcagcaaaaa gcaattatcg ctcgtgaagt cgaccgagat cctgatctgt tgattgttgc
    14161 aaatccaact cgtggactgg atgtaggtgc gatcgaattt attcataaac gtctgatcga
    14221 acaaagggac aaatacaaag cagtgttact gatcagtttc gaattagaag aaattttaaa
    14281 tgtttcggat cgtattgctg ttatccatga aggagaaatt gtcggtatcg ttgatccgaa
    14341 agaaacatct gaaaatgaat taggtctatt gatggcgggc tataccttag aagaagcacg
    14401 aaaagagtta gaaaaggggg ctggcgcaca tgaatgatcg cagcgaaaaa ttccgtaatt
    14461 tattagtgcc tgtactttca gttgttttag gattgatctt aggcgcgata ttgatggctg
    14521 cttttggttt tcagcccatc caaggttatt cttctatgct agatgcagcc cttggttcgc
    14581 aaagagggat aggtgaaacg ttaaggcaag caacgccgtt gatttttaca gcattaggtt
    14641 tttctgtagc taactcagct gggtttttca atattgggct ttctggacaa gcactttgcg
    14701 ggtggatcgt cagtgtctgg gtggcacttg cgtttccaga tctgccaaaa gttgttttgc
    14761 ttccgttatg cgtgatctta ggtgccttag ctggtgcgat ggcagcggct gttccaggtt
    14821 tacttcgagc attttttgga acaagtgaag tgatcgtaac aatcatgatg aactatatct
    14881 tattatacgt aagcacattt gctttacatg aagtgatgcc tgaaagctat cgctcaagtt
    14941 tggactcttc caatatgatc agtcaaaatg cctcactgcg gttaccatgg ttaacacaag
    15001 cttttggcgg ctctcgcgta aactcaggat tatttttagc aatcatcgcg cttattttcg
    15061 tttggctgct gatgaagaaa acgactttag gatttgaaat ccgttctgtt ggattgaatc
    15121 cttttgcttc tgagtatgca ggaatgagta gtaaacgtac gatcgttttg tcgatggtca
    15181 tttctggcgc tttggcaggg cttggcggtg tggttgaagg attaggaacg tatcaaaact
    15241 tctttgttca aacgacttcg ctatctattg gatttgacgg aatggctgtt tccttgcttg
    15301 gttctggatc agcaatcggt attcttttat cagcgatctt gttcagtatt ttgaagatcg
    15361 gtggacttgg catgcagaca ggagcaggtg ttccgtttga gatcgtcaat gtatcgattg
    15421 ctttgattat cttctttgtc gggatcaact tcctgattcg tttcgtcatg gctaaagtct
    15481 tccaagggaa aaaacaagaa gagatcgttg cgacaataga atcgaaacca acgaatcaaa
    15541 accatgaagg gggcgaacta taatgtcgac tatcgaatta attgccttga tccttacttc
    15601 tacactggtt tattcaacac cgctaattct aacgtcacta ggcggaactt tttctgaacg
    15661 aagcgggatc gtaaacgtag ggctagaggg aatcatggtc atgggtgctt ttagttccat
    15721 cgttttcaac ttgacgatga gtccatcact tggttctatg acaccatgga tcgctatttt
    15781 agtgggtggc gtcgtaggta tcctattttc tctgattcac gcagtagcaa cagtcagttt
    15841 gcgtgctgac catattatca gcgggacagt aattaatctg atggcacctg ctttaggtgt
    15901 tttcttgatc aaagcatggt atggtaaagg tcaaactgat aacatcacgc agaatctggg
    15961 gtatttttct ttcccaggat tagcagatat tccggtcatt ggaccaattt tctttaaaaa
    16021 tacattctta cctgcttatt gtgctatttt gattgctgtc ctagcttggg tgatcatctt
    16081 caagacgaaa ttcggcttgc gcctccgttc agttggtgaa aatcctcaag ctgccgacac
    16141 attaggtatc aatgtatatg ctatgcgtta ttcaggcgta ctgatttctg gcttgttagg
    16201 cggtatgggc ggtgctgttt ttgctcagtc gatttccggt aacttttcag ctggtacgat
    16261 cgttggacaa gggttcatct ccatggctgc tatgatcttt ggtaagtgga atcctttagg
    16321 ggcaatgggt gcctcattgt tctttggttt tgctcaaagc ttgagtatca ttggcgcaca
    16381 attaccagtt atttcttcga ttccgccagt attgctgcaa attgctccat atttgctgac
    16441 aatcattgtg ctggttgttt tcttagggaa agcttctggt ccaaaagcaa acgggaagaa
    16501 ctatatcaaa tcaaaataat cttttagcga ataaaaaggg tgggttacgg gaagtgtttt
    16561 tcgtctcgcc ctttttgttt taaaggcaat aaaaataaaa agaaagaagg aataatcatg
    16621 tttaaacgcg tacatctgat cgtcatggat tcagtaggaa ttggagaagc accagatgcc
    16681 gataagttcg gtgatgttgg ttctgataca ttaggacaca ttgcgaaaga agcaggattg
    16741 aacgttccac atttagagca gcttggttta ggaacgatcc gtcctcttga aggtgtggaa
    16801 tcagtgaaag atcatcaagg ctatgcgacg aaattagaag aagtttctgt agggaaagat
    16861 acgatgacag gccactggga aatcatggga ttgaatatca aaactccatt tcgtgtgttt
    16921 cctaatggat tccctgatga tctgatcaaa caaatcgaag aattttctgg ccgtaaagtc
    16981 gtatgtaaca aaccttatag cggaacagct gttattgacg attacggtga acaccaaatg
    17041 aaaacaggcg atttaattgt atatacttct gccgatcctg tccttcagat cgcggcacat
    17101 gaagaaatca ttcctttaga agaattatat cggatttgcc aattcgttcg tgatataaca
    17161 aaagatgatc cgtatatgat tggccgaatc attgcacgtc cgtacgtggg tgaaccagga
    17221 gacttcacac gaacaagcaa tcgccatgat tatgcgttga atccattcgg acacacagtc
    17281 cttgattctt tgaaagaagc aggaaaggac gtgattgctg ttggaaagat caacgatatt
    17341 ttcaacggac aaggaatcac tgaatctgtc cgcacgaaaa gcaatatgga tggcgttgac
    17401 cagctgctga aagttatgaa aaacgatttt tcaggaatca gctttaccaa tttagtagat
    17461 tttgatgcat tatacggtca tcgtcgggat gtagtcggct atgcgcatgc gattgaagaa
    17521 tttgacttac gaattccaga attgcttgaa gcgatggaag aagacgactt gctaatgatt
    17581 acagccgatc acggaaacga tccgacgttt actggtaccg atcatacgcg agaatatgta
    17641 ccgttattgg tttacagtaa aaaaatgaaa gaacaagggg cattgcctca aggatattat
    17701 tcagatattt cagcaacgat tgctgagaac tttagtgttt cttcgactga aaatggcgaa
    17761 agcttcttga aattattaaa ataatctttt taaataggag aaagcaaatg accaagttaa
    17821 gcgagatgtt acaagaaacg acccagttta tccttgataa aggagtaaaa gaaattgatt
    17881 ttggtttgat tctaggttca ggattaggtg aattagcaga tgaaatcgaa gatgctgtaa
    17941 tgatttctta tgatgagatc ccgttctttc ctgtttcgac agtagttggg catgcaggaa
    18001 agctagttta tgggtctctg gcaggcaaga aagtgttagc catgcaaggg agattccact
    18061 actatgaagg tcattccatg caaacagtga cctatccagt acgtgtgatg gccggactag
    18121 gggcccattc agtcattgtg actaatgcat gtggtggtgt caacgaatca tttgtaccag
    18181 gagatttgat gctgattacc gatcatatca attttactgg tgaaaatcct ttgatcggtc
    18241 cgaatgaaga tgcaatgggc cctagatttc cagatatgag tcaagcttat acgcctgact
    18301 atcaagcaac agcaaaagca gcagcagaaa aactaggttt ggatcttaaa gaaggcgtat
    18361 atatgggcta ttctggacca acttatgaaa cgcctgcgga gatccgaatg tctcggacaa
    18421 taggagccga tgcagtaggg atgtcgactg ttccagaagt catcgtagca gctcacagcg
    18481 gattaaaagt gttaggaatc tcttgtatta ccaacttagc agcaggtatg caagcgaatc
    18541 tgagccatga agaagttgta gaaacaactc agagagtcaa acaatctttt aaagcattgg
    18601 ttaaagaaac attgacatta ttgtaatagg aggaacaata aaatgagtgt gcatattgaa
    18661 gcgaaagaag gcgagatcgc cgataagatt ttacttccgg gagatccttt gcgagctaaa
    18721 tatattgcgg agactttttt ggaagatcca gtttgttaca atcaggtacg cggtatgtta
    18781 ggctttaccg gcaaatataa aggagaacgt atttctgttc aaggaacagg aatgggaatg
    18841 ccatcagcca ccatctatgc ccatgaattg atccagtcgt atggtgtaaa gaaattgatt
    18901 cgagtaggta catgcggtgc attgtcaaaa gacgtccatg ttcgtgattt agtactagca
    18961 caaggtgcag cgaccagttc ttcgatgatc gaaaaaaatt tccaagcctt tcattttcct
    19021 ccaatcagtg atttcaatct attactgaaa gcatatgaga tagctaaaga aaagggctat
    19081 acggttcatg taggaaatgt tctttcggaa gattctttct acaaagatga tttgacagag
    19141 acgttccaat tagctgaact tggcgtttta ggagtggaaa tggaagcagc ggctctttat
    19201 tacttaggcg cgaaatacca tgtgcagaca ttaagtttga tgaccgttag cgaccatttg
    19261 atcaccggtg aagaaacaac agctgcagaa cgtcaatcta cgttcaatga aatgatcgaa
    19321 gtaggattgg aaacagcgat agcagaataa gaaggaaatg aaaaagctgc cctgcattaa
    19381 gtaataagaa catccaagca aaaatagctc ctcatgtttt tgacttggct ggacttatta
    19441 ttgcaaatgt aagcttttga acaccgttta ccaagaggtt gtgaatctgc cgtttaactc
    19501 tgagtcacaa ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg
    19561 tgaccgagaa gttggcagga gaaaaccgtt tatacgaaaa aagggactgt gacaaatcgt
    19621 gtcacagtcc cttttttata gtgtttttta taggtagtat tttttagcaa ctgttaagtc
    19681 atcatttaat tcataaacaa gtggttgacc tgttggaatt tcaagatcca tgatgtcttc
    19741 gtcagagata ccttcgatat gtttagctag ggcgcgtaat gagttaccat gtgcagctac
    19801 taaaacagtt ttgttgtcta gtaatgctgg agcaatttca tcttgccaga atggcaaagc
    19861 gcgttctaat gtcactttca agttttctcc accaggaata tcgcgtttgt ctaacattgc
    19921 gtaacgacgg tcatttgctg cagaaccttc gtcagtttct tccattaatg gaggaagagt
    19981 atcgtaagaa cgacgccaga tgtgcacttg ttcgtcgccg tatttttcag ctgtttcttt
    20041 tttgttcaaa ccttgtaatt taccatagtg acgttcgttt aagcgccatg atttgatttg
    20101 tggtacccaa agttgatctg aatattctaa gatcaagtta catgttttga ttgcacgagt
    20161 taaaacagaa gtgtaagcaa catcaaattc gattccagct tctttgattt tgcgtccgcc
    20221 ttcttttgct tcttcgattc cttctggtgc tagatttaca tcagcccaac cagtaaattg
    20281 gttcaatgca ttccattcac taagaccgtg acgagaaaaa actaattttg gcatgtttag
    20341 ctctcctttt aagataggtt tatatttctg aaattttcag ttctgtttta ttgtacaatt
    20401 ttttggaaga gatttccatc gaaaaattag ataagattct atgagaaaac aagtagagaa
    20461 tataaaaaaa cgaacagtgc ccacgcaact gttcgttttt aggtattaga tgcataattg
    20521 ctcgatttct ttttcaggta aattgatata tttataacat gttccaactg aaacaccgca
    20581 ttttgaagaa ataaattgga tcgtttcttt tttttcataa tagagacgtc ggattttttt
    20641 gatcgttttt gcatcgattt ttggtcttcc gccgattttt ccttttttac gagcttcatc
    20701 taatccaact aatgtacgtt ctttgatcaa tgcgcactcc atgtttgcta ggttttccat
    20761 taattggaaa tagatttttc ccatttcgcg cgtatcgatt ccttcatcaa gactaatcaa
    20821 gtgcagcccg cgatctttga ataattgcgt gagttctgtt aattgccgcg tagatttgcc
    20881 taatcgatga agttggcaaa tgactagtgc atcgccttgt tgcatgtcag cgatcactga
    20941 ctccaaatga acagcagtag aatcattttg ttccacttca tatgtctcat ggaaaatttc
    21001 ttgacaacca aagtcagata attgatcaac ctgagttgtt aaatcaacgt catttatggt
    21061 tgtgcgtgca taaccaataa ttttcatggt gattcctttc tagcagtgat tttctaactg
    21121 gatataacag taaaaatgta gcataaagta cgtcaaaaag atagtttttt cgcaaatttt
    21181 ccgctgtctt tttttattgg atgatcaagc agtttttctt tatttacgac ggattgcgtc
    21241 aagaatctca acgccttttt ctgagccgac aactacttta gaaaccatgt ttaggaaaag
    21301 cccatgttct actaccccaa caagttcatc taaataggta gctaagcttt ctggctgttc
    21361 gatgacttct agatgcagat caatgatgta atgaccacca tctgtggtta agacttctcc
    21421 gttttcgtct gtacgcaaga cgggttggaa tcttttttct tcaaaaatat gaagtagctg
    21481 ttgggagcca taagggatca cttcaactgg gagtgggaac ttcccaagtt tatcgactaa
    21541 tttagaagaa tcaacgatcc aaatcgtttc ttttgagtac gtagcgacta ttttctcgaa
    21601 aagcaaagca gcgccgccgc ctttgatccc ttgaaaatca gcactgattt catcagcacc
    21661 gtctattgtc aaatcgacta cggggacttc atcaatactc tttaacggta ttcctaattt
    21721 ttccgcttgt tcttttgtcg catttgaagt agtaactcct gtgatacgta aaccttcttc
    21781 acgcatacgg cgaccaatct cttctaccat ataataggcg gttgaaccgg tcccgagacc
    21841 tacgatcatc ccgtccttta cgaatttcgc ggcttcaatg ccagctaatt gttttaaatt
    21901 catctttgcg tccacctttt tttagtttga gcatcgtgct ttcctcacta ttttagcgtg
    21961 atttacggta aaaagatacc attctttttt attccaataa agctttttca aaaaaaattg
    22021 catctcttgt tgacatgttt tttcattcgt gatattctaa caaaggtgct ttatctacag
    22081 gtttctgtga agaaacgtgc tgactgttta tcaaagcata tttgataagg atgcagacat
    22141 tgcatttatt ttttatcaca aaaaaagaac cacctggatg tgtggaacta gacgcaaatc
    22201 gtgggaagga ggaaacaagg aatgcctaca attaaccaat tagtacgtaa acctcgtaaa
    22261 tcaaaggtgg aaaaatctaa ttcacctgct ttgaacaaag gatataacag ctttaagaaa
    22321 actcaaacta acgtaaactc acctcaaaaa cgtggggtag caactcgtgt gggtacaatg
    22381 acacctaaaa aaccgaactc agctttacgt aaatatgcac gtgttcgttt gtcaaactta
    22441 attgaagtta ctgcttatat cccagggatt ggtcataact tacaagaaca cagtgttgta
    22501 ttattacgcg gtggtcgtgt aaaagactta ccaggggtac gttaccatat cgttcgcggt
    22561 gctttagata ctgctggtgt taacgaccgt aaacaaagcc gttctaaata cggaactaaa
    22621 aaacctaaag cttaatttta attattaaac aaatgaaact tgcttctact atatagaagt
    22681 aattactatg aacaatattt cggaaggagg agttacggat gccacgtaaa ggtcctgttg
    22741 caaaacgtga tgttttacca gatcctattt ataactcaaa attagtaact cgcttgatca
    22801 accgtgtaat ggttgacgga aaacgcggga ttgcagctaa cattatctat aatgcctttg
    22861 acttgatcaa agaatctaca ggtaacgatc cattagaagt gtttgaacaa gcaatgaaaa
    22921 acgttcaacc tgttcttgaa gttaaagcac gtcgtgttgg gggttcaaac taccaagtac
    22981 cagttgaagt tcgccctgaa cgccgcgtta ctctaggtct tcgttgggta gtaaactacg
    23041 ctcgcttgcg cggtgaacac acaatggaag aacgcttagc aaaagaaatc atggatgctg
    23101 caaacaacac aggtgcttct gttaaaaaac gtgaagacac tcataaaatg gctgatgcca
    23161 accgtgcgtt tgctcactat cgctggtaag attctctctg tttggtcggc ttttataagt
    23221 cgacctcaga tagtgtcctt atttttagca actagatagt aaagagagga gaaacaagga
    23281 gaatgaccag agaattttct ttacaaaaca ctcgtaatat cggtatcatg gcccacatcg
    23341 atgctggtaa aacgactgct actgaacgta tcttgtacta tactggtaaa atccataaaa
    23401 ttggtgaaac tcatgaagga gcttcacaaa tggactggat ggaacaagaa caagaacgtg
    23461 gtattacgat cacttctgct gctactacag cacaatggaa gggtcaccga atcaacatca
    23521 tcgacacacc aggacacgta gacttcacag ttgaagttga acgttcattg cgtgtacttg
    23581 atggtgctgt aactgttctt gatgcacaat caggtgttga acctcaaact gaaacagttt
    23641 ggcgtcaagc aacaacttat ggtgtacctc gtatcgtatt tgcaaacaaa atggacaaga
    23701 tcggtgctga cttcttatat tcagtatcaa ctttacatga ccgtttgcaa gcaaacgctc
    23761 acccaatcca attaccaatt ggtgcggaag acgacttcac aggaattatc gaccttgtaa
    23821 caatgaaggc tgaaatgtat acaaacgatc ttggaacaga gatcgaagaa acagaaattc
    23881 ctgaagaata ccgcgaatta gctgaagaat ggcgcgaaaa attggttgaa gctgtagctg
    23941 aaactgatga agaacttact ttgaaatact tagaaggtga agaaatcact gaagctgagt
    24001 taaaagaagg tatccgccgc gcaacagtta acgtagaatt ctacccagta ctatgtggtt
    24061 cagcatttaa gaacaaaggg gttcaattac tattggacgc tgttcttgat tacttgccat
    24121 caccgttaga tatcccagct atcaaaggga ttgaccctaa aactgatgaa gaagtagaac
    24181 gtcctgcaga cgacagcgct cctttctcag ctttagcatt taaagttatg actgacccat
    24241 tcgtaggtcg tttaactttc ttccgtgtgt actctggtgt tttacaatca ggttcatacg
    24301 tacaaaacgc aacaaaaggc aaacgtgaac gtgttggtcg tatcctacag atgcatgcca
    24361 actctcgttc agaaatctct gaagtttacg ctggagatat cgctgctgct gttggtttga
    24421 aagacacaac tactggggac acactatgtg atgaaaagag tctagttatt cttgaatcca
    24481 tggaattccc agaaccagtt atccaagtcg ctattgaacc taaatcaaaa gctgaccaag
    24541 ataaaatggg tgtggcttta caaaaactat ctgaagaaga tcctactttc cgtgctgaaa
    24601 caaacgtgga aacaggcgaa acaatcatcg ctggtatggg tgaacttcac ttagatatca
    24661 tcgttgaccg tatgcgtcgt gaattcaaag ttgaagctaa cgtaggtgcg cctcaagtat
    24721 cttaccgtga aactttccgt gctggcactc aagccgaagg taagtttgta cgtcagtctg
    24781 gtggtaaagg tcaatacggt cacgtatgga ttgaatttac accaaatgaa gaaggtgctg
    24841 gtttcgaatt tgaaaatgcg atcgtcggtg gggttgttcc tcgtgaatac attccagctg
    24901 ttgaaactgg tttgaaagat gctatggaaa atggtgtctt agctggctat ccattagtag
    24961 atatcaaagc taaactttac gatggttcat accatgatgt cgattcaaat gaaactgcct
    25021 tccgtgttgc tgcttctatg gcattacgtg cagcagctaa gaaagctaat ccagtaatcc
    25081 ttgaaccaat catggctgta gaagttgtta tccctgaaga ttatttaggt gatgttatgg
    25141 gtcacgtaac tgctcgtcgt ggacgtgttg aaggtatgga agctcgcgct ggcggccaac
    25201 aagttgttcg tgcgatggtt ccacttgctg aaatgtttgg ttatgctaca actcttcgtt
    25261 ctgctactca aggtcgtggt acatttacaa tgacatttga ccactacgaa gatgtaccaa
    25321 aatcagtaca agaagaaatc attaagaaaa acggcggaaa agctgaataa tttcttaaaa
    25381 ccggatagtc ctgcaaagag attatccaaa gagactatat ttttaatata gtttcgtgta
    25441 aaataattat cgatgatatg ggcatgtggt gtaccttgtg tacatcatat acctatcaga
    25501 caaataaaaa atatattgta tttttaggag gacattttaa aatggcaaaa gaaaaatttg
    25561 accgttctaa accacacgtt aacattggta ctatcggaca cgttgaccat ggtaaaacta
    25621 cattaacagc tgcaatcaca actgtactat ctaagaaaaa cggcggccaa gctatggctt
    25681 acgatcaaat cgatggtgct ccagaagaac gcgaacgcgg aatcacaatc tcaacagctc
    25741 acgttgagta tgaaacagat acacgccact acgctcacgt tgactgccca ggacacgcgg
    25801 attacgttaa aaacatgatc actggtgctg ctcaaatgga cggagctatc ttggtagttt
    25861 ctgctgctga cggcccaatg cctcaaactc gtgaacacat cctattgtct cgtcaagttg
    25921 gtgttcctta catcgttgta ttcttgaaca aagtagacat ggttgatgac gaagaattac
    25981 tagaattagt tgaaatggaa gttcgtgacc tattaacaga atacgaattc cctggtgacg
    26041 atgttcctgt agttgctgga tcagctttga aagctctaga aggcgacgct tcatacgaag
    26101 aaaaaattct tgaattaatg gctgcagttg acgaatacat cccaactcca gaacgtgaca
    26161 acgacaaacc attcatgatg ccagttgaag acgtgttctc aattactgga cgtggtactg
    26221 ttgctacagg tcgtgttgaa cgtggacaag ttcgcgttgg tgacgaagtt gaagttgttg
    26281 gtattgctga agaaacttca aaaacaacag ttactggtgt tgaaatgttc cgtaaattgt
    26341 tagactacgc tgaagctgga gacaacattg gtgctttact acgtggtgtt gcacgtgaag
    26401 acatccaacg tggacaagtt ttagctaaac caggtacaat cacacctcat acaaaattct
    26461 ctgcagaagt atacgtgttg acaaaagaag aaggtggacg tcatactcca ttcttcacta
    26521 actaccgtcc acaattctac ttccgtacaa ctgacgtaac aggtgttgtt gaattaccag
    26581 aaggaactga aatggttatg cctggcgaca acgtaacaat ggaagttgaa ttaatccacc
    26641 caatcgctat cgaaaacggt actaaattct caatccgtga aggcggacgt acagttggtg
    26701 ccggtgtcgt tacagaaatc aaagcttaat tcacttaagt tttaaagtaa agcaacattt
    26761 attcggacgt aatcgctcgg aacgaaggtc ttaggatctt tgttccgagc ttttttattt
    26821 attgcaggat caggaaaaaa gacaggagta ttataaatag aagaaacacg aaataaaaga
    26881 gtcagtattt tctaaatgaa actttatgaa aacaaaatgt tttttacaag aaaaagcctt
    26941 gctttgtgag tttatatata ttataatgac aaaggtgcgt ttactttagt gaattgcaca
    27001 gacgtaatca gaatgatttc gtcggctaag aaacgagagg ttgcgacacg cccggacgct
    27061 ttgccacgaa cgagcgtgac ggaaattttc gtggagctat gtctactttt caaaataggc
    27121 gaaggaggga acaagatggc aaaacaaaaa attcgtatcc gtttaaaagc gtatgaacac
    27181 cgtattttag atcaatcagc ggataaaatc gtggaaactg caaaaagaac tggagctagc
    27241 gtgtctggtc caattccatt accaactgaa cgcagtcttt acacaatcat ccgtgcgact
    27301 cataaataca aagattcacg cgaacaattc gaaatgcgta cacacaaacg tcttatcgac
    27361 attgtgaacc caacaccaaa aactgttgat gctttgatga agctagactt accatctggt
    27421 gtaaacatcg aaatcaaatt ataaaactaa aacaaaaata atggaggtgt actcatgacc
    27481 aaaggaatct tagggaaaaa agtgggaatg acacaaatct tcactgaatc tggtgaatta
    27541 attccagtaa ctgttgtaga agctactcca aacgtagtac tacaagtgaa aacaatggaa
    27601 acggatggct acgaagctat tcaagttggt taccaagata tgcgtgaagt tttatcaaac
    27661 aaacctgcga aaggtcatgt tgcaaaagca aacacggctc ctaagcgctt cattcgtgaa
    27721 ttcaaaaatg ttgagctagg agaatatgaa gtaggaaaag aaattacagt agatgttttc
    27781 caagcaggag acattattga tgtaacaggt actacgaaag gtaaaggatt ccaaggggtt
    27841 atcaaacgcc acggacaaag ccgcggacca atggctcacg gttctcgtta ccatcgtcgt
    27901 cctgggtcaa tgggtccagt tgcacctaac cgtgtattta aaaacaaacg tcttgctggc
    27961 cgtatgggtg gcaaccgcgt aactatccaa aacttggaag ttgtacgtgt cgatgcagaa
    28021 agaaatgtta tcttgatcaa aggtaatgtt cctggagcta aaaaatcttt aattactatc
    28081 aaatcagctg tgaaagctaa ataattgtag gaagaaagga ggaactaagg aatgccgaat
    28141 gtagcattat tcaaacaaga tggaagccaa aacggtgaaa tcacacttaa cgaagaaatc
    28201 ttcggaattg aacctaatga aagtgttgtt tacgatgcaa tcgtaatgca acgtgcgtca
    28261 ttaagacaag gaacacacgc ggttaaaaac cgtagcgctg ttcgcggcgg cggacgtaaa
    28321 ccatggcgtc aaaaaggaac aggtcgtgca cgtcaaggat ctatccgctc accacaatgg
    28381 cgtggaggtg gcgtagtctt tggaccaaca ccacgttctt acagctacaa acttcctaaa
    28441 aaagttcgtc gtttagcaat gaaatctgta ttgtcagaaa aagttgctga aaacaacttg
    28501 gtagcaatcg aaggcttgaa cttcgacgca ccaaaaacaa aagaattcaa acaagttctt
    28561 gctaacttat ctatcgactc taaagtattg gttgttctag aaaaaggtaa cgactttgct
    28621 gctttatcag cacgtaactt accaaacgtt tctgtagtaa cttctgataa tgtgagtgtg
    28681 ctagacgttg tatcaaatac aaaagtgtta gcaacacaaa ctgctcttac tcaaattgag
    28741 gaggtgcttg cataatgaac ttactagacg taatcaaacg cccagtgatc actgaaaaat
    28801 caatgcttgc catggatgac aagaaataca cttttgaagt ggacactcgc gcaaacaaaa
    28861 ctttagtaaa acaagcggta gaagctgctt ttgacgttaa agtcaaaaac gtgaacatcg
    28921 tgaacgtacg tgcgaaattc aaacgtatgg gcaaatatgc aggatataca aaaaaacgtc
    28981 gcaaagcgat cgttacattg actgaagatt caaaagaaat ccaattattc gaagctgctg
    29041 aataagcact aatagataac tcattaaatt aggagggaaa aacgtggcga ttaaaaagta
    29101 caaacctacc tcaaatggtc gtcgtaacat gacaggttct gattttgctg aaatcacaac
    29161 atcaacacct gaaaaatcat tgttacaacc attgaaaaac catgctggac gtaacaacaa
    29221 cggccgcatc actgtacgtc atcaaggtgg cggacacaaa cgccaatatc gcgtgatcga
    29281 cttcaaacgt aacaaagata acgttgttgc aactgttcaa acgatcgaat atgatccaaa
    29341 ccgttcagct aacatcgcgt tagtacacta tgaagatgga gtgaaagctt acatcttagc
    29401 acctaaagga ctagaagtgg gtatgaaact tgtttcaggt ccagaagcag atatcaaagt
    29461 cggaaatgct cttccattag aaaacattcc agttggtact gttatccaca acattgaaat
    29521 gaaacctggt aaaggtggac aattgatccg ttctgctggt acaagtgctc aagtacttgg
    29581 taaagaaggc aaatacgtat tgatccgctt gaactctggt gaagttcgta tgatcttagc
    29641 tgcatgccgt gctacaatcg gttctgtagg taacgaacaa cacgaattaa tcaacatcgg
    29701 taaagctgga cgctctcgtt ggatgcgtaa acgcccaact gtacgtggta gcgtaatgaa
    29761 ccctaatgat cacccacacg gtggtggtga aggtaaagct cctatcggac gtaaagcacc
    29821 agtttctcct tggggtcaac cagctcttgg ttacaaaact cgtaacaaaa aagctaaatc
    29881 agacaaactt attgttcgtc gtcgcaaaaa taaataatta ctttgaccat gtgtcgcaca
    29941 ttttgagagg aggttcacca tgggtcgtag tttaaagaaa ggaccttttg tcgatgagca
    30001 tttaatgaaa aaagttgaag ctcaagcagg ggttgaaaag aaaaaagtta tcaaaacttg
    30061 gtcacgccgt tcaacagttt tcccaagttt tgttggatat accatcgcag tttatgatgg
    30121 acgtaaacat gtaccagttt acatccaaga agacatggta ggacacaaat taggtgaatt
    30181 tgcacctaca agaacttatc gtggacatgc tgctgacgat aagaaaacga aacgctaatt
    30241 tgaggggagg ataaatccaa atggcagaac aaatcacatc agctaaagca actgcaaaaa
    30301 cagttcgcac ttcacctcga aaagcccgtt tagtaattga ccttatcaga ggtaaaagtg
    30361 tcgcagatgc aatttcaatc ttgaaattca caccaaacaa atctgctgga atcattgaaa
    30421 aagtattgat gtcagcagtt gcgaatgcag aaaataactt tgacttagat gttgaaaact
    30481 tggtagtaag cgaagctttt gttaacgaag gacctacaat gaaacgtttc cgtcctcgtg
    30541 caaaaggatc agcttctcca atcaacaaac gtacaagtca tatcacggta gtcgtatcag
    30601 aaaaataagg agggataatc agtgggtcaa aaagtacatc caattggaat gcgtgtaggc
    30661 atcatccgcg actgggatgc taaatggtat gctgaaaaag agtatgctga gttcttacac
    30721 gaagatttaa gaatccgtaa atttatttca actaaacttg ctgatgctgc tgtatcaaca
    30781 gttgaaatcg aacgcgcaac aggtcgcgta aacatttcaa ttcacacagc taaaccaggt
    30841 atggttattg gtaaaggcgg ttctgaagtc gaaagcctaa gaaaagaatt aaacaaatta
    30901 actggtaaaa gaatccatat caatatcgtg gaaatcaaaa aaccagatct agatgcaaaa
    30961 ttagtaggtg aaggaatcgc acgtcaatta gaaaaccgtg ttgctttccg tcgcgctcaa
    31021 aaacaagcca tccaacgctc aatgcgtgct ggcgctcaag ggatcaaaac tcaagtatct
    31081 ggtcgtctaa acggagcaga tatcgctcgt tcagaaggat actcagaagg aactgttcct
    31141 cttcatactt tacgtgcgga cattgattac gcatgggaag aagcagatac aacatacgga
    31201 aaactaggag ttaaagtgtg gatctatcgt ggagaaattc ttccaactaa aaaaaacact
    31261 gagaaaggag ggaaataatc atgttagtac ctaaacgtgt aaaacaccgt cgtgaattcc
    31321 gcggaaaaat gcgcggggaa gctaaaggcg gaaaagaagt agcattcggt gaatacggtt
    31381 tgcaagctgt tgattcacat tggatcacaa accgccaaat cgaagctgct cgtatcgcaa
    31441 tgactcgtta catgaaacgt ggtgggaaag tatggattaa aattttccct cacaaatctt
    31501 atactgccaa agcaattggg gtacgtatgg gttctggtaa aggggcacct gaaggatggg
    31561 ttgcaccagt aaaacgtggt aaaatcatgt ttgaaatcgc aggcgttcct gaagaagtag
    31621 ctcgtgaagc gttacgtcta gcttctcaca aattaccaat gaaaactaag atcgtaaaac
    31681 gtgaggaaat gggtggtgaa tcgaatgaag gttaaagaaa tcagagaatt aaccactgcc
    31741 gaaatgcttg atcaagaaaa acaattaaaa gaagaattgt ttaatcttag attccaatta
    31801 gcaacaggtc aattagaaaa cactgcacgt attaaagaag tacgtaaatc gattgcacgc
    31861 atcaaaactg tgttgcgtga acaagctaag taattatgga aggaggccat taagcgtatg
    31921 actgaaggaa gaaatcaacg caaagtttat caaggtcgcg tggtttcaga caaaatggat
    31981 aaaacaatta ccgttgctgt cgaaacaaag aaaaaccacc ctatctatgg caaacgcatg
    32041 aactattcta agaaatacaa agtgcatgat gaaaacaaca cagcaaaagt tggcgacatc
    32101 gtgagaatta tggaaactcg tccattatca gctacaaaac gtttccgttt gttggaagta
    32161 gtcgaagaag cagttattat ctaataacac gagattttcc tatatagatt gaatgcaaaa
    32221 tctgaaagga ggatacacaa cgtgatccaa gcagaaagtc gtttaaaagt cgctgataac
    32281 tcaggcgcac gtgaaatttt gacgatcaaa gtccttggtg gatctggtcg taaaactgct
    32341 aacatcggtg atgtgatcgt tgctacggtt aaacaagcaa cgccaggtgg ggttgtcaaa
    32401 aaaggtgacg tcgttaaagc cgttatcgtt cgaacaaaat caggagctcg tcgtacagac
    32461 ggttcatata ttaaatttga tgaaaatgct gcagtgatta ttcgtgatga taaaagccct
    32521 cgtggaacac gtatctttgg gccagttgca cgtgaactac gtgaaaacaa cttcatgaag
    32581 atcgtttcac tagcaccaga agttttataa tcttgagaca cttatcaaag gaggtgcgaa
    32641 acagttatgt ttgttaaaaa aggcgataaa gttaaagtta tcactggaaa agacaaaaac
    32701 aaagaaggcg ttgtactagc agcgttcccg aaaaaagata aagttattgt cgaaggtgta
    32761 aatgtcgtta aaaaacacca aaaaccttct caagctgctc ctcaaggtgg aattgttgag
    32821 atggaagcgc caattcatgt ttctaacgtg atggtggttg actctactgg tgtagctggc
    32881 cgagttggct acaaagaagt agacggcaaa aaagtccgtg tttctaaaaa aaccggtgaa
    32941 gttttagata aataaaagtt taaggaagga gggtcttact gaatgaaccg cctaaaagaa
    33001 aaatatctta aagaagtaac tccatcattg atggaaaaat ttaactatag ctctgttatg
    33061 caaacaccta aagttgaaaa aatcgtcatc aacatgggtg ttggtgatgc ggtatcaaac
    33121 gctaaaaact tagataaagc agttgaagaa ttaactttga tcactggtca aaaaccaatg
    33181 attacaaaag ctaaaaaatc aatcgctgga ttccgtttac gtgaaggaat gccaattggt
    33241 gcaaaagtta cattgcgcgg agaaagaatg tacgaattct tagacaaatt ggtttctgtt
    33301 tcactaccac gtgtacgtga cttccatggt gtaagtaaaa aagcctttga cggtcgtgga
    33361 aactatactt taggtattaa agaacaattg atcttcccag aagtagatta tgatttagta
    33421 gataaagtac gtggtatgga catcgttatt gtaacaacag caaactctga tgaagaatct
    33481 cgtgagttgt tgacacaatt aggcatgcca ttccaaaaat aattaaagga ggcgaactac
    33541 gtggctaaaa aatcaatgat tgctaaaaac aaacgtcctg caaaattttc aacacaagct
    33601 tatactcgtt gcgaacgttg cggacgtcca cattcagttt atcgtaagtt ccatctttgc
    33661 cgtatttgct tccgcgaact tgcctataaa ggtcaaattc ccggcgtgaa gaaagctagc
    33721 tggtaaaaag taaattcgca taagaaggag gtaaaagatc aatggtcatg acagatccaa
    33781 ttgcagattt tctaactcgt atccgtaacg caaacatggt taaacatgag tctttagaag
    33841 tacctgcatc aaaaattaaa cgtgacatcg ctgaaatctt gaaacgtgaa ggtttcgttc
    33901 gtgatgttga atacatcgaa gatgacaaac aaggcgtgat ccgtgttttc ctaaaatttg
    33961 gtaaaaacgg cgaacgtgtt atcacaaact taaaacgtat ctctaaacca ggtttgcgtg
    34021 cttatgtcaa agctgacgaa gtaccaaaag tattgaacgg tttaggtatc gcaatcattt
    34081 cgacttctga aggtgttgtc actgataaag aagctcgtga aaaaaatatc ggcggcgaag
    34141 ttatcgctta cgtatggtaa tcaagtagaa tctacaagga ggtgtcttta agtgagccgt
    34201 atcggaaata aagtcgttgt tatccctgaa ggagtaacag ttactcaaga cggaaacaac
    34261 gttacagtta aaggaccaaa aggtgaattg actcgtactt tttcagcaga tatcaaaatg
    34321 aatatcgaag gaaacgaagt aactttcact cgtccaaacg acagtaaaga aatgaaaaca
    34381 attcatggaa caactcgtgc gaacttcaac aacatggttg taggtgtcag cgaaggtttc
    34441 caaaaagcgt tagaactaat cggggttggg taccgtgcac aattacaagg atcaaaactt
    34501 gtattgaacg ttggttactc acatccagta gaaatcacac caccagctgg agtaactgtt
    34561 gaagtaccag ctaacacaca agtaattgtt aaaggtgcga acaaagaaga agttggcgaa
    34621 ttagctgcaa acattcgtgg aactcgtcct ccagaacctt ataaaggtaa aggtattcgt
    34681 tatgttggcg aatatgtacg ccgtaaagaa ggtaaaactg gtaaataatc gtttagaaaa
    34741 attgcataga agccagcagc aggaaaaagg catttggctg aagtttacct tcaaaaaaat
    34801 acatttttac ttgtgccgca gcttctattt ttcgaaacgt atttactgct tgaaatagta
    34861 gtacaataaa aataaagagg tgacaattgt gattacaaaa ccagataaga acaaaacacg
    34921 tcaaaagaga catcgtcgtg tacgtaacaa tatctctggt actgctgagc gcccacgctt
    34981 gaacgttttt cgttctaaca aaaacatcta cgctcaaatt attgatgacg tagcgggtgt
    35041 aacgctagca agtgcctcta ccttggataa agaaatttca ggtggaacaa aaacagaagc
    35101 agcacaagct gtcggtaaac tagttgctga acgtgcagct gaaaaaggca ttaaagtagt
    35161 agtctttgac cgtggtggat acctttacca tggccgtgtg caagctttag ctgaagctgc
    35221 tcgcgaaaat ggactagaat tttagaagaa ggaggaatac cattcatggt ttatatcgat
    35281 ccaaaacatt tggaattaga agaccgcgtt gttgcaatta accgtgtaac aaaagttgtt
    35341 aaaggtggac gtcgtctacg ttttgctgct ttagttgttg tcggtgacaa aaacggacac
    35401 gtagggtttg gtacaggtaa agctcaagaa gttcctgaag caatccgtaa agcaattgaa
    35461 gatgcgaaga aaaacttagt tgaagtacct atggttggtt caacaatccc acacgaagta
    35521 atcggtgctt ttagtggtgg tagaatccta atgaaacctg ctgtagaagg ttctggggtt
    35581 gctgctggtg gaccagttcg tgccgtatta gaattagctg gggtagcaga tatcacttct
    35641 aaatctttag gctcaaatac accaatcaac gttgttcgcg caacagttga aggtttgaaa
    35701 caattgaaac gtgcagaaga agttgcagaa cttcgcggca aatcagttga agaattaatc
    35761 ggataaggag gacaacaata atggctgaat taaaagttac tttaaaacgc agtattatcg
    35821 gacgtcctca aaaccaacgc gatacagtta aagcgttagg tcttacaaag attaacagtt
    35881 cagtggttaa gccttctaat gaagcaatta aaggcatgat caatactgtt tctcacctag
    35941 tggacgttga agaaatttaa tttagaaaaa tcataactag aacattaagg aggtgccaaa
    36001 cctatgaaac ttcatgaatt aaaacctgct gaaggttcac gccaagtacg taaccgtgtt
    36061 ggtcgtggta cttcatctgg taacggaaaa actgccggac gcggacaaaa aggacaaaaa
    36121 gctcgttcag gcggcggtgt gcgtttagga tttgaagggg gacaaacacc attgttccgt
    36181 cgtttaccaa aacgaggatt cacaaacgtg aaccgtaaag actatgcagt cattaatctt
    36241 gatgtattga accgctttga agacggagct gaagtaactc ctgttgcttt agttgaagct
    36301 ggaatcgtga aaaacgaaaa agctggaatc aaagtattag ctaatgggga actaaacaag
    36361 aaattaactg tgaaagcagc aaaattctct aaagcagctc aagaagcaat tgaagcggct
    36421 ggtggatcaa tcgaggtgat ctaatgttta gactattaaa agatgctttt aaagtaaaag
    36481 acattagatc gaaaatccta tttactgtat ttgtgttgtt tgtatttcgc ctaggtgcac
    36541 atattactgt accaggcgta aatgctgctt cattgcaaaa cctttcttct ttgccatttt
    36601 tcaacatgtt gaacttggta agtggtagtg cgatgcaaaa cttttccatt ttctcaatgg
    36661 gggtttcacc atacattact gcttctatcg tcattcagct tcttcaaatg gacatcgtgc
    36721 ctcgatttgt tgaatggtcg aaacaagggg aagttggtcg taaaaaactg aatcaagcga
    36781 ctcgatatct gacattagta ttggcattta tccaatctgt cggtatcact gctggattca
    36841 atcaatacgc tcaattaggt ttcgtgaata atcctaacgt agcaacttac gtaacgatcg
    36901 gtttgattct aacagccggt acgatgtttg ttacttggct aggagaacag atcacagaaa
    36961 aaggtatcgg aaatggcgta tcaatgatca tttttgcagg tatcatttct agattgcctg
    37021 aaagtatcag agaaatttat gaagattact ttataaatat tgatccttca gacatatgga
    37081 aatctgctat ttttgtggcc attttactag tagcggtctt gttgatcgtg acattcgtaa
    37141 catttttcca acaagctgaa cgcaagatcc cgatccaata tacgaaacgc gtagctggcg
    37201 cgcctacaag cagctatctg ccgttgaaag taaatgcagc tggagttatt ccagttatct
    37261 ttgctagttc gtttattgct acgccaaatg ctgttttaca agcattgagc ggtagattcg
    37321 gttctgaaaa ctggtacaaa gtgatgatgg agatctttaa ctataataca gcacctggtg
    37381 ctacgattta tacgattctg atcgttgcct tcactttctt ctacgccttt gttcaagtaa
    37441 accctgagaa attagcggaa aacttgcaaa agcaaggaag ttatatacca agcgtacgcc
    37501 ctggtaaggg gactgaagag tatgtctcaa gattactgat gcgtttaagt acagttggtg
    37561 cagtcttcct tggtttagtg gcattgcttc caatcattgc tcaaatggtt tgggatcttc
    37621 cacaatcaat cggattaggc ggtacaagct tactgatcgt tatcggtgta gctttagaaa
    37681 ctgcgaaaca gttagaaggc ttaatgctga aacgtaaata tgttggattc attgagtaag
    37741 aaagcgtgtt gaggtttccc ctcaacacac gttctcacac atttattagg agggaaaaca
    37801 atgaacctca ttttaatggg gctgccaggc gcaggtaaag gaacacaagc tgaaaaaatc
    37861 attgatgctt atggtattcc gcacatctca acaggagata tgttccgcgc agcgatgcaa
    37921 aacgaaacag ctcttggcct ggaagcaaag tcttatatgg ataaaggtgc attagttcct
    37981 gatgaagtaa caaatggaat cgtgaaagag cgactagctg aaccagatac agagaaaggc
    38041 ttcttattag atggctttcc acgtacgcta gatcaagcaa aagctctgga cgcaatgcta
    38101 aaagatttga ataaaaaaat tgatgctgtc atcgatatcc atgttggtga agaaatcttg
    38161 gtcgaacgtc tagccggccg atttatctgc agcaattgtg gagcaactta ccataaaatt
    38221 ttcaacccta caaaagttga agacacatgt gatcgttgcg gtgggcatga attctatcaa
    38281 agagaagatg ataaacctga gacggttaaa aatcgtctgg ctatcaacat caaaaatagt
    38341 gaaccaatct tggcttatta taaagaacaa ggtttgctaa acactattga tggtgctcgt
    38401 gaaatcgatg ctgtatttgc agatgtacaa aaaatcattg aaaaatagca cgattgcccg
    38461 tagccgactt gcattatgca gttactcttg tcaagttcaa taaattatga tagaatatgt
    38521 cagtccgact tctagagaat cctctagatg atccattttg agaattttga ggtgggaact
    38581 gcagtttccg ccattttatc gtgtgaaaat gcgaaacaag tacaaggagg tactgtgcgt
    38641 ggcaaaagaa gatatgattg aagtcgaagg tacagtcgtc gaaactttgc cgaatgcaat
    38701 gtttaaagtt gaactagaaa acggacacca agttcttgct actgtttcag gtaaaattcg
    38761 tatgcattac atccgtatct tacctggtga caaagtaacg gttgaattgt ctccatacga
    38821 tcttaaccgt ggccgtatta cttatcgctt taaataattg tactccgtaa aactacagga
    38881 ggtattatca tgaaagtaag accatcagta aaaccaatgt gcgaacattg taaagtaatt
    38941 cgtcgtaaag gacgcgttat ggtgatttgc ccagcaaatc caaaacataa acaacgtcaa
    39001 ggataattgg aggtgctata aatatggctc gtattgcagg agtagatatt cctcgtgata
    39061 aacgtgtagt agtttcactt acttatatct atggtattgg taacactact gctaaaaaga
    39121 tcttagctga tgctggtgta tctgaagatg ttcgcgttcg tgaattaacg aatgaacaaa
    39181 cagatgctat ccgtgcagaa gtagataaat taaaagttga aggggatctt cgtcgtgaag
    39241 tgaacttgaa catcaaacgt ttgatggaaa tcggttctta ccgtggaatc cgtcatcgcc
    39301 gtggattgcc tgttcgtgga caaaacacga aaaacaatgc acgtactcgt aaaggcccag
    39361 ctcgcgctat cgctggcaag aaaaaataat tcttaagtga aggaggttaa aacttcatgg
    39421 cagcaaaaaa agtgaatcgt aaacgccgtg tgaagaagaa tatagaagca ggtattgcac
    39481 atatccattc tacattcaat aacactatca tcatgatcac tgatactcac ggaaatgcat
    39541 tagcgtggtc atcagctggt tcattaggtt tcaaaggaag caaaaaatca actccttttg
    39601 ccgctcaaat ggcagcagaa actgctacaa aagcagcaat ggaacacgga ttaaagactg
    39661 ttgaagtaac agttaaagga cctggttctg gacgtgaagc agcaattcgt tcattacaag
    39721 ctacaggttt agaagtgact gcaattcgtg acgtgactcc agttcctcat aatggtgccc
    39781 gccctccaaa acgccgtcgt gtttaatgag tgccgctcat tttgagtttt atcagtaacg
    39841 tcattgaaca agacactttt cgttttgaaa ggggtaaaga taagaatgat tgagttcgaa
    39901 aaaccaagaa tcgcaaaaat tgatgaagaa aaagattatg gcaagttcat cgttgaacct
    39961 cttgaaagag ggtatgggac tactttaggg aattccctac gtcgtatttt attatcttct
    40021 ttgccaggtg ctgccatcac taatattcaa atcgatggtg tactgcatga attctctacc
    40081 atcaaaggtg ttagagaaga cgtcactcaa atcatcttga acattaaagg cttagcgctg
    40141 aaaatgtatg gccaagaaga aaaaaccctt gaaatcgata ttaccggacc tgctacagta
    40201 actgctggcg atattatcgt tgatagtgat gtagaaattc ttaacaaaga tatgtacatc
    40261 tgtagtgtct ctgaaggagc tacgttccat gctcgcttaa cagtgaaacc tggacgtggt
    40321 tatgttcaag cagatgaaaa taaaaaagaa gatatgccaa ttggcgttct tccagtcgat
    40381 tcaatctaca cacctgttcg tcgtgtgaac tatcaagttg agaacacacg tgttggacac
    40441 cgtgaagatt ttgataaatt gacgatggag atttggacag atggttcaat tgaaccgttg
    40501 gaagcaatga gtttgtctgc gaaaatcatg actgaacatt tggatatttt tgtgaactta
    40561 actgatgaag caaaaaatgc tgaaatcatg gtcgaaaaag aagaaacaca aaaagaaaaa
    40621 atgttggaaa tgacgatcga agagttagac ttgtctgttc gctcttacaa ttgtttgaaa
    40681 cgtgcaggaa tcaatactgt acaagaactt acaaataaat ctgagcctga aatgatcaaa
    40741 gtccgtaact taggacgtaa atcattagaa gaagttaaag caaaattgca cgacttaggt
    40801 ttaggtttac gtaaagaaga ttaaaacctt ttacgaagga gggaaaccaa cgtgagttat
    40861 cgtaaactag gacgcacatc tagccaacgt aaagcgatgt tgcgtgattt aacaactgat
    40921 ttattaatca acgaacgtat tgtgactact gaagctcgtg cgaaagaagt tcgctcaact
    40981 gcagaaaaaa tgatcacttt aggtaaacgc ggagatttgc atgcacgtcg tcaagctgcg
    41041 gctttcgtac gtaatgaagt tgccagcgtg cgtgaagaaa atgaggacat cgtcattgaa
    41101 tcagctttac aaaaactgtt caacgatatt gcacctcgtt acgcagaacg tcaaggcgga
    41161 tacacacgca ttttgaagac agaaccaaga cgcggtgatg gtgcaccaat ggttgtctta
    41221 gaacttgtct aaaatcacac taataacatc actttataga tgattctttt agagtgttat
    41281 gatgttggaa gatatttttc cgagtctagc tcagcgctgc tcctcaagcg aatatcgctt
    41341 ggggagtaag cactcatcat aaagtgtttt tttatacctt caattacctg tcatgacaga
    41401 tatgtttgaa ggcataaaaa aacattttaa gcgagaaata attgaaaatt ttgtcttttt
    41461 ttgatagtgt tagtaggaac tttaggatga agaaggagta atcatggagc caattattga
    41521 attagaaaag ataaattata aataccagcc agatgatctt cgccccgcat tgaaagatgt
    41581 ctcctttaca attgataaag gtgagtggat cgctattatt ggacacaatg gttcaggaaa
    41641 atcgacactt gccaaaacga tcaatggatt gcttctgcca gaatcaggga tcgtgaaagt
    41701 gggaaatcaa atattggatg aagaaaatat ttggacgatc cgacagatgg ttggaatgat
    41761 ttttcaaaat ccagacaatc agtttgttgg ttcgacagta gaagatgacg ttgcttttgg
    41821 tcttgaaaat caagggattc ccagagaaga aatgctagtg cgtgtgaaag atgcacttga
    41881 gaaagttaga atggctgagt ttgcttcacg cgagcctgct cgtttatcag gagggcaaaa
    41941 acaacgtgtt gccattgccg gcgtggttgc cttacgacct gatatcatta ttttagatga
    42001 agcaaccagt atgctagatc cagaaggtcg tgaagaagtg atctctacga tcaagaagat
    42061 caaagaagaa agtcaattga ccgttatatc tatcactcat gatattgatg aagcagctaa
    42121 tgccaatcgg atactagtga tgagacaagg cgaacttgtc cgtgaaggta caccaaaaga
    42181 aattttttct gctggtcctg aactgatcga cttaggtcta gatctaccat ttcctgaaaa
    42241 actgaagagc gcactgaaag aacgtggggt tgatgtgccc agtgaatata tgacagaaga
    42301 aaggatggtg gattggttat ggacatccgt tttgaacaag tagacttcac ttatcagccg
    42361 aatacaccat ttgaacaaag agccttattt gacatcaata tgacgatcaa agaaaacagt
    42421 tatactgcat tagtagggca taccggaagc gggaaatcaa ccttacttca gcatttgaat
    42481 gcgcttgtta agccaacaag cggtacagtc catattggag aacgagatat tcagccggat
    42541 acggataata agaatttaaa gcccatccga aaaaaagttg gcatcgtctt tcagtttcca
    42601 gaagcacagc tattcgaaga aacggtagca aaagacattg ctttcggtcc taaaaacttt
    42661 ggtgtcagcg aagaagaagc actagtccta gcaaaagaaa cattagaaca agttgggctg
    42721 gatgaaagct atttggaacg ttcgccattt gaactttcag gagggcaaat gcgtagagta
    42781 gcaatcgctg gtgtgcttgc catgaggccg gaagtacttg tattggatga accaacggca
    42841 ggacttgatc cccagggcag aaaagaaatg atggagatgt tttggcgtct tcataaggaa
    42901 cagcagatca ctattgtatt agttacgcat ttaatggatg atgtagccaa ttttgcagat
    42961 tatgtctatg tgttggaaaa gggacgaatc gttaatagcg gggagccgca agaggtcttt
    43021 caaaacattg aatggctaaa agaaaaacaa ctaggcgtac ctactgccac cgagtttgca
    43081 gaagaattaa tggctaaagg gatgaacttt gctaccttgc ctttgacagc tgaagaatta
    43141 gcagacgcta tcgtccatta tgctggagga aatgcccatg atgaataaat tgatatttgg
    43201 tcgttatatg cctggagatt ccttgatcca tcgattggat ccccgagcta aattattagc
    43261 cagtttttat tttatcggaa tcattttttt agcaaataat tgggaaacgt accttttgtt
    43321 ggcatttttc acattacttt ctgtcttctt gtcaaaagta gatttgaaat tttttatccg
    43381 aggcattcgc ccattgatct ggctaatcct cttcactgta gctttgcagg tcttgtttac
    43441 aagtggcgga gaaacttatt ggagttgggg tgttttccat atcactgaat ttggtgtgat
    43501 gaacggcctc tttattttct gtcgattcgt gttgatcatt tttatgtcga ctctactcac
    43561 gcttactaca ccgcctttgg aattatcgga tgcgattgag tacttgcttc gtccattgaa
    43621 agccgtacgg ttcccagtcc acgaaatttc attgatgctg tcgattgctc ttcgttttgt
    43681 gcctacattg atggacgaaa cagaaaagat catgaacgct cagcgcgctc gcggtgttga
    43741 ttttggtgaa ggaaacctag ttcagaaaat gaaagcagtc attcctttgc tgattccttt
    43801 atttgtcagc agcttcaacc gtgcggaaga tcttgcgact gctatggaag caagaggcta
    43861 caaaggtggg gaaggaagaa cgaaatatcg tgtattgcat tggcataata gagacacgat
    43921 ggttatcatt gcttttgtat tattaactgt gctactgatc tttttaagag gataaacgtt
    43981 cagtgggccg ctatctattc ttttcttgct gatctgtttg caagaaagga tgcaagcagt
    44041 gtatgacaag aaatattagg gtctgtggca gactaaggtc acaggcctct ttcttttatc
    44101 tgaaaagaaa gaacgaaaaa attttataga aaaatcatta ttccagataa ccttttagga
    44161 acaaaggaga gaaaaacatg gtacgttaca aagcaatcat tgcgtacgac ggaacaaatt
    44221 ttaatggatt ccaaaaacag ccaaatgggc gtactgttca agaagaagta gaaaaaacat
    44281 tacaaaaaat ggcaaatgga aaagaaatca cggtctttgg ttcgggaagg acagatgccg
    44341 gagtacatgc gatcgggcaa gtaatccatt ttgattatcc agaggagcgt ccgttagaac
    44401 gaatgcgttt cgcgctagat acccagtcac cagaggatat tgctgtaaga caagtagaaa
    44461 ttgtttcaga agacttccac gcaagatatt tggtcaaaga aaagacgtat caatttcgag
    44521 tggatatagg gaaaccacgc agtccttttc gacgccatta tgcaagttat tttccttacc
    44581 cacttgattt ggaaaaaatt cagcgggcgc ttcctgattt attagggact cacgatttca
    44641 cctctttttg tgcatcagga agctcgatag aggataaaat acgtacgatc tacgaggcta
    44701 agatggaggt aaatgaaaca aaagacgaat tgttgtttac atttagggga aatggcttct
    44761 tgtataagat gatacggatc ttagtaggaa cgcttttgaa aattggcaat ggccgattgc
    44821 ctgaagatag tatcccagat attattgcga aaaaagatcg taatgcagca ggacctacag
    44881 cacaccctga aggactttat ttgtatgaag tgaaatatga ataagaggtt atgatagaag
    44941 tggtcagctt caagaaataa gaaggtattt acgaaaatta cttctcaaaa ttttgcaaaa
    45001 tttcggctta ttttcgaaga agctacttct ggaacaccgt ttaagaaaga ggtcgtgagc
    45061 ctgtcattta actctgagtc acaaagagca atttcaaaaa acagcttctc atctttgatg
    45121 aatatcgtga cttgtggacg aggcgttgat aagtgaatca tctttttgtt acctattttt
    45181 tctgtaacca aaaaacgatg gaacgttgtt ttgattgccc ttggtgcgga cagtctgaac
    45241 aggttccatc gtttctttat tttctacgtc ttcttctgtg tgtgtttcgt ggttgttctt
    45301 ctattgctgg atcaatcgtg ccataaaagg ggcgattgcg gtcaaggtac cagacataaa
    45361 gaataaacaa tgcaatacat aggacaaaaa ggattgcacc tatcgtaaat ccttcaggca
    45421 agtaactgaa agtgacagta tgtttacctg ctggaacttt tatcatcaag aatgcgtctt
    45481 tgaaattgct gaccttgact ggtttcccat caattttagc tttccatcct ttgtcataag
    45541 gaatagttgt caccaacatg gaatcttcag cggtatttac agtcccagaa gcttttctgc
    45601 cagtggcttc catttcgaca cctttttctt tgatctgatt cattgcggct tgataggcta
    45661 caacatctag accaacgact ttaggctcca tgaaacttat ttctttcgtc ccataaaaac
    45721 ttgcagtaaa tgtcactgtt gtctcttgag gataatagcc aagatcatag tattgaccag
    45781 aaatgttgat ttgcgttttt cgcgatgtac cattgacagt gatcgtcgca ctagaacttt
    45841 ctaattgcgc aaaattggtt gggaacaaac ttaagtaagc ttgtgtattt gcaggcacat
    45901 ggacttccca agtgagctct tttggctggt tatctgctgt ttctgagtaa gtgacgccag
    45961 tcgttgtctt tgtgatcgtc gtattcaact gattcttcaa agtaggtgta tagaatgtaa
    46021 aatactgttc atttagacca gacaatgtat tgatcaagtt tgtctgactg gtcaaattat
    46081 ctgtcactgg ctgctcaaca tcattaacgg acaacggtgc agtaaatgct agtggcaatg
    46141 cattgacatt ctgatacagt gtatagtcgc cgctttgacc agcttggcgg aagccatatt
    46201 tactgaacgt gctttcgttt ttagaaatgt tatatttgat tcctgtaaac ccatccatca
    46261 gcaaagtatt attgttataa cggatattca aatttgtccc tcttgaacgg aaacctaatt
    46321 tatccaggta agaggaagaa ttacgatttc ttatagaaga aaacaaactg ataccactgt
    46381 aaccatagtt gatcgaatcg tttgcagata ctggatccag attttccaag cggtaaaacg
    46441 agtcggaatc tttttttgtt tcatttacaa gagctttcaa gtcaggataa ggatcagtat
    46501 ataaactgcg ggaagcataa ttccaatcat caagaatgcc atggatcata ctgttcgtat
    46561 tgaccagtgc ttcagcagac atgatcaata ataacaatat actcagatga ttgaatgaaa
    46621 gttttttatg ctgataaaat cccacagcaa aagcataaat ccctaagaaa accactgtca
    46681 atacaaagga agtcatcgtc acataggtgt agcttccttc aggtttgatg ccccatgcca
    46741 ccgcaaagag agcagctacg accaaaatga ttcctaacaa cagcccacca tcgtcttgtt
    46801 ctatcttttc ccatcctttt cctgcaagca ggagcactaa gaaagagaac agataggcat
    46861 aacggaataa aaacatattc ggtgcatgca taccatgcca gaacaaattc aatggaacaa
    46921 gataaaaact tgcgataagt atggcgaaaa gactgccgta caaaaattta tttttcaacg
    46981 gtactttccg actggcaaaa taaaacagac agaggactaa tggtagcaga cctgcgtaaa
    47041 cgaacggaat cgatccgtat tttgtcgtgt cataaacacc aatcatattt ttcatgatta
    47101 gatccagata agaagtcgct tctgttttca acgtagtgat ctgactcagt gcctcgccat
    47161 ttgaccgaag atccagcact gctggcaata caatgatcat tgaagcgccg cctgccagta
    47221 aagaagtgat accataagga acaatcgttt ttttatattc tgaccagttt gtcaatagac
    47281 gggcgataaa atataagaaa gaaaatacgc caatcataaa acccatataa aaactggata
    47341 aaaacaagag taaataactg ataaataaaa gtttcggccg tttaagatcc ataacacgat
    47401 gaatccccaa aatcactaat ggaagataaa caaatgcatc cagccacatg atgatctccg
    47461 aatgtgcagt gataaatgag atcaatgcgt aactgacgct aagtgcaaca tgcccccagc
    47521 gaggaatctt gaacgtacgt gcaagaaacc aaaaagccaa tccggcacag ccgaccttaa
    47581 gcaaagtcag aacatacagt gcatctggca tcatctggtt tggaaaaaag aacaccagtg
    47641 gcgtgaagat cccgcctaga tagtaagaaa ttaaagataa ataattcaat cctagagaag
    47701 cattccatgt ataaaaaata ctttgctttc caagaagaac atttcggaag ctggcatgaa
    47761 aattagaaaa ctgtgaaaag gcatcacttg ccaaaatact tctgctgctt ccaggataga
    47821 ttccgatact tagataaaca agtgctaaaa tcaaaaacgg aatcaggaaa ctagctgctg
    47881 cgtagcgtcc gttttctcta aagaatgact ttaaattttt tttcatattt attttccacc
    47941 aaatttctat gaagttgtat tcagtttatc atatccttgt ttttctctgc ttaaaaagag
    48001 tctagttttc acaaaaaaat aaaatttaga ataaataact tcataatttg ttcgtataac
    48061 aaaaataaac gcaaaaaaag ggtaggaatc aaaagtccca cccttaaaaa tttttattaa
    48121 ataaaaaacc gttcactatc tgaacggtcc aagtatcatt caattaaacg cgttcaactt
    48181 ttcctgattt caaagcacga gttgacaccc aaactttttt aggttttccg tcgaccataa
    48241 cgcgaacttt ttgtaagtta ggtttaacag tacgttttgt tgagttcata gcgtgtgaac
    48301 ggttgtttcc actttttgtc ttgcgacctg taaagtaaca aacttttgcc atggtaattt
    48361 cctcctttgc cttgatttgg agcaatagaa taattcagct cataccagac taacatatca
    48421 tatagaatgg gaatttgcaa gtacattttc atagatacaa ttgacaattg agaagaataa
    48481 ttgtaaatta aacaaggtta tacctgaaat tttgcaaggt ttttgagtac tttgggtaac
    48541 tgccttttat ttcaagttgc gctatgataa aatatgatag aagaaaaatg ggagtttttt
    48601 acccgttaag gaggctcatt catggctgta aaaattcaaa cgtcagctgg aaccattgaa
    48661 attaccaatg atgtaattgc aacagttgtt ggtggcgccg cgacagatgt gttcggtgtt
    48721 gtaggaatgg ccagcaaaaa tcaaattaaa gataatatta ctgagatttt gcgtaaagaa
    48781 aattactcaa gaggagtagt tgtccgccaa gaagataatg gtattgctgt agatgtttat
    48841 acgattatca gctatggtac gaaaatctct gaagtttccc gcaacgtcca agaaaaagtg
    48901 aaatacaatc tagaggcatt attaggcgta actgccaatt cagttaatgt ctttgtacaa
    48961 ggtgttcgtg tactgccaga ctaggcagga gactggctgg taactcagca gtcgatctga
    49021 aggaggatta tctaggtgag tgtaacagaa ataagtgcaa gccagttcca ggaaatggtc
    49081 caggcaggtg cgaatcgtct gcaagtaaat gcagaatacg tcaattcatt aaatgtattc
    49141 cctgtgccag atggcgatac gggaacaaat atgaatctat ctatgactag tggagctaag
    49201 gcagtagtag attcaacctc agaaaaagta ggagaattag cagcaatgct ttcaaaagga
    49261 ttgctgatgg gtgcgcgcgg aaattccggc gttattttgt ctcaattgtt tcgtggattt
    49321 tctaagcaaa ttccagatgt tgtctcatta aatgcttctg atcttgctgc agccttcact
    49381 catggagttg aaactgcgta taaagcagta atgaaaccag tagaaggaac gattttgaca
    49441 gtagctagag aagcagcgaa agctggagaa aagaaagcaa aaagtactga tgatgtcgtt
    49501 gaagtaatga cagctgttgt aaaaggagca aagcgtgctt tagctaaaac tccagacttg
    49561 cttccagtcc taaaagaagt cggtgtcgta gacagtggag ggcaaggact gctgtttgtt
    49621 tatgagggct ttttaagtgc attgaatgga gaattccaag cggatgaaac atatgaacct
    49681 tctccagcag aaatggatga tatggtcaat gcagaacatc atcgcagtgt ccaaggtcaa
    49741 atggcgacag aagatatcca ttttggttac tgtacagaaa tcatggtaaa aatcggagaa
    49801 ggacctacag tcgatagtac attcgattac gaagaattcc gtaactattt agatggtata
    49861 ggagattctt tacttgtggt caatgatgac gagatcatca aagtccacgt gcatacagaa
    49921 aacccaggag aagtcttgaa ttatggacaa aaattcggtt cattagtcaa aatcaaagtc
    49981 gacaacatgc gcttgcagca tgagacgatt ttggaacacg atgaagaagt gaacgagttt
    50041 gcgaaaaaag aaataccacg tatcccgtat ggtatcgtag cgatcgctgc tggtaaaggc
    50101 gtccaagagc tgtttgaaag tttaggcgct aattatgtta tcagtggtgg acaaacaatg
    50161 aatccaagta cggaagacat tgttaaagcg attcaagaag taaacgcaga taaagtgatt
    50221 gtcttgccaa acaataagaa catcttcatg gcagctgatc aagcagcaga agtagcagat
    50281 atcccagtag cagttgttcc ttcaagaacg atttcccaag gattgacagc tatgctttca
    50341 ttcaatgatc aagcttcttt agaagaaaac aaagaaacga tgacagaaat gttgtctagc
    50401 gttgtgagcg gtcaagtcac tcacgcgatc agagatacct caattgacgg tgtagagatc
    50461 aaagaaggag actttttggg catgatcgat ggaaaaatcc tgatctctaa cccagatatc
    50521 acagcaacag cgttaagtac actagaacaa atgatcaatg aggacacaga gatcgtaacg
    50581 atcttggcag gtgatgaagg aacagttgaa caagcaaatg aatttgctga gaaactaaca
    50641 gaaaaacatc ctgatttgga aatcgagatc caccaaggag atcagcctgt ctatccttat
    50701 ctattttctg ctgaataatt tctgtgtcag aatatctttc taacacagta ttcattcata
    50761 aaaggccttc ggttagtgat caggtatgat cgctgaccaa gtcctttttt tgttaaagga
    50821 gggaggaaaa atttatgcgt tctttagatg atccaatcac tatgctggca ggagtcggag
    50881 aaaaaagagc agctagtctt gccagcttgg gcatccaaac gattgaagac ttactgactt
    50941 attatccttt tcgctacgaa gatattcagg aaagaaatct aaacgagatc caggatcaag
    51001 aaaaggttac tttgaaagga atcgtagtct cgcctcctgt gatgaatcga tttggttata
    51061 aaaaaagccg attgcaattt cgtatgatgc aagaacatga tgtgttcaac gtttcttttt
    51121 ttaatcaacc ttatctaaaa gaaaaagtca ttttatcgga ggaaatcgcc gtatacggta
    51181 aatgggatgc aaagcgtaaa gccttgaacg ggatgaagat tttaggttct caaacaatgg
    51241 atgacttttc gccgatatat cacgtcaaca aatcaattcg tcaaacgaca ttagttgatc
    51301 taatcaaaaa agggtttcaa gaatacggca cattgattga agaaaatctg ccaaacaacc
    51361 ttgtggagaa atatcgactg cttgaccgag cttctgctgt aagaagtatg cattttccaa
    51421 aagaccacga agaaaaccat caagccaaac gacgtgtcgt ttttgaagag ttctttttat
    51481 ttcaaatgcg tcttcaagga ttgaaaaaag cagaaaaagc agaaacgaat ggtttggaga
    51541 tcctttatga tgtccaaaaa ttgaaacaat ttactcaaaa gctacctttt gaactgacaa
    51601 atgcacaaaa acgtgtaacg aatgaaatct gtcgggatct tcgcagtccg cagcatatgc
    51661 agcgactttt gcagggagat gtaggtagcg gaaaaacagt ggttgccgcg attgcgttat
    51721 acgctaccgt tacagcaggt ttccaaggtg cgttgatggt gcctacagaa atcttggcac
    51781 aacaacatat ggaaagcttg caacagctgt ttgatcccaa tgaagtaacc atcgcattga
    51841 ttacggggtc aacgaaagca aaagaacgac gtgagctcct tgaaaaacta gagcaagggg
    51901 aaattaacgt cgtgatcggc acacatgcat tgatccaaga tggagtcgaa tttcagaatt
    51961 taggtctagt tattacggat gagcagcacc gttttggcgt gaaccagcgg aaagtacttc
    52021 gtgaaaaagg ctggcgacca gatgtcttat ttatgacagc gactccgatt cctagaacat
    52081 tagctattac agcatatggt gagatggatg tatctatcat tgatgaactt ccagctggac
    52141 gtatcccgat cgaaacacgc tgggtaagaa cacctcaatt ggacagtgta ctggattgga
    52201 cgaaaaaaga actggcaaga ggacatcaaa tgtatgtcat ctgtccgctg attgaagaat
    52261 cagaagcatt agatgtaaaa aatgcagtgg aaatctatga aaaacttcgt gaactatttg
    52321 caccaaaata tgaagttggc ttgttacatg gtaaaatgaa gaatcaggaa aaagaagtaa
    52381 tcatggaagc cttcaaagac aaccagatgc agatattagt ttcgactact gtaattgaag
    52441 taggggtaaa cgtgccgaac gcgaccgtca tgctgatcat ggatgcggat cgatttggat
    52501 tggcacagct gcaccaactt cgtggacgtg ttggacgcgg aagtgatgct tcctactgta
    52561 tcttagttgc taatcctaaa aatgaactag gtgtggaacg gatgaaaatc atgacagaga
    52621 ccaccaatgg ttttgtacta agcgaaaaag atctggaatt acgtggtcct ggtgaagtat
    52681 ttggcttccg tcaatctggt ctgcctcaat ttgcgattgc tgacttggtg acggatggaa
    52741 atgttttgga ggtagccaga gaagaggctg cagccctttg gcaaataaag gattggcaga
    52801 tgcttcctga gtatcagcct ttagcgcaaa gcctccaaat agaaaatcag gataatcgtt
    52861 atttcgatta gcagactgtg ctatacttta tactgatgac ggaaaaagga gtggaaacaa
    52921 tgaaaattgc cgtagatgca atgggcggag atcacgcacc tcaagcaatt gtagaaggcg
    52981 tggctttagc gcagcaagat tttccagaag tagaattctt actttacgga aaagaagcag
    53041 aaatcaagaa atacttaaca aatgaaaaaa acatcacgat tatccataca gacgaaaaaa
    53101 tcaatagtga tgatgagcca gtaaaagcaa ttcgacgaaa aaagactgca tcaatggtcc
    53161 ttgctgcaca agcagtgaaa aacggtgaag cagatgctgt attttcagca gggaatacag
    53221 gcgcgctact agcagcaggg ttgttcatcg taggacgtat caaaaatatc gaacgccctg
    53281 gcttgatgtc taccttgcca gtagtaggga aagaaggtgc cggtttcgat atgctggatc
    53341 ttggcgctaa cgcagaaaac aaaccagaac atttgttgca atacggtatt ttaggttctt
    53401 tttatgcacg taaagtccga ggtatcggac gtcctcgtgt cgcactctta aacaacggaa
    53461 cagaagcaac aaaaggtagt gaagtgacga aaaaagccta tgaactcctt cagaatgaaa
    53521 caagcttgaa ttttattgga aacgtagaag ctcgtgagct actcaatggt gtggctgatg
    53581 tagtggtcac agatggattt acaggcaacg ctgttttgaa atctattgaa ggaacagcaa
    53641 tgaatatgat gaatctatta aaatctgcta ttctaaatga agggatcaaa ggaaaaatgg
    53701 gcgctatgct tttaaaagac ggcttacgtt ctttgaaagc agaaatggat tattccaaac
    53761 atggcggtgc cgttttgttt ggcttgaaag caccagtcat taaaactcat ggagcaacag
    53821 gccccgatgc tgttcgctat acgatccgcc aaatccacac aatgcttgag acagatgtag
    53881 ttggacaatt agtcgaacaa tttgaaaaaa gcgaagaata gtatttggat atctgaagcg
    53941 atccgttcca ttgaaaaaaa ggtggacatt cctgaaaaat gccagtaaaa ttagccatgg
    54001 tgtaggtaat tgacaagtgt atgattcttg gaggtgaaaa agttgactcg cgaagaagta
    54061 tttaacaaag tagcgaaaat tatttcaaat cattttgaga tagataccga taaagtgacg
    54121 gatgaattga acatcaaaga tgatttaaaa gctgattcaa ttagtattat ggaattcgtt
    54181 ttagagcttg aagatgaatt cggtacggaa atttcagatg aagacgctga acaaattgaa
    54241 acagtcggtg gcgcagtaga ttatatcagc agccatctta aataatttca aataaaagca
    54301 aagaaggata aaaagctgca tgcagctttt tatccttctt tttttaatct ttttgacacg
    54361 taaaagtcat ttttctgata attctggcga taaacagaaa ttatgttgca aagtaaagcg
    54421 gttttctata taattattaa taattcatta cacaaagata agaataacct ttgttataaa
    54481 acgatttatt ctgtgtaatg tcctttttta ttaagaaaaa cttccattta ggaagcagag
    54541 aggggtattc gattgtccga taaccaatta ttagaagttc agaacctaca tacaggattc
    54601 cgactcaaag atgactatta cgatgcagta gatgacgttt catttacttt agacaaaaac
    54661 gagattttag caatcgttgg ggaatctggg tgcgggaaaa gtaccttggc tactacgatt
    54721 atgggattac ttgatcctaa taacacaaaa attacaggtg agattttata taacgatctg
    54781 aatttaatcg acctgaacga aacgctctat aataaaatcc gcggaaatga tatcggaatg
    54841 attttccaag atccgttgtc tgcattaaat cctctcatga gaatcgaaga ccagatcaaa
    54901 gaaggacttt cctatcacac aaagatgaat gcagaacagc gtcaagctcg tgcactagaa
    54961 ttgctagaac aagtagggat tcctaatcct gcgagagttg gacgccaata tccccatgaa
    55021 ctatcaggcg gtatgagaca acgtgtcatc atcgcaatcg caatcgcctg caaacctcca
    55081 atcgtcattg cagatgaacc gacgacagcc ctagatgtaa ccatccaagc acagattctt
    55141 gatttactga aagaccttca ggaagaaaca aaaacaggga tcatcttgat cactcatgac
    55201 ttaggagtcg tagcagaaat ggctgatcga gtggcagtga tgtatggcgg acagttcgtt
    55261 gaagtagcag gtgtaaaaga actgtttgat catcctaagc atccttatac acgctcttta
    55321 ctgaactcta taccacaaga aggaaatcat gatgcagaac tccatgtgat cgaaggtgtc
    55381 gttccttctc tgaaaaacat gcctcgtaaa ggttgtcggt ttgcaccacg tattccatgg
    55441 ataccagcaa gcgatcatga agaagagcct atccttcatg aagtcgcacc gaaccacttg
    55501 gtacgttgct cctgctataa gcatttccac tttagagacg aaaaaggaga ggtgtagatg
    55561 acagaattaa tcacaatcaa agacctgaaa gttcattacc ctattcgcag tggattcttc
    55621 aatcgagtaa cggatcatgt atatgctgta gatggtgtgg attttattat cgaaaaagga
    55681 aagacatatg gtttagtggg agagtctggc tctggaaaat ctactacggg aaaagccgtt
    55741 gtgggattag aaaaggtaac agccggacaa atcatgtatg aaggaaaaga tgtaacgaag
    55801 cgaagcaacc gcaaaaaaat gggttataac aaggatgtgc agatgatctt ccaagattca
    55861 atgtccagtt tgaatcctaa aaagcgagtg ttggatatca ttgctgaacc catccgaaat
    55921 tttgaacggt taagcgatca agaagaaaag aaaaaggtaa aaggactgct agatatcgtt
    55981 gggatgccgg aagatgcgct atataaatat cctcatgaat tttcaggcgg acaacgtcaa
    56041 cgtctaggag tagcccgtgc tgttgctacc aatccaaaac tgatcgtagc cgatgaacct
    56101 gtatctgctc tggatctatc tgttcaagcg caagtattga atttcatgaa acgaatccag
    56161 caagaatacg gcctcagcta tttattcatt tcacatgatt taggtgtagt aaaacacatg
    56221 tgcgacaata tcgcaattat gtacaaaggt cgatttgtag aaatcggtac aagagaagac
    56281 atttataatg acccgcgaca tatctatacg aaacgtctgc tttcagctat tccgcaaatc
    56341 gatgtagaaa atagggaaag ccacaaagaa aatcgacgaa gagtagaaca agagtatatc
    56401 caaaaccaaa aagaatacta tgatgcgact ggtcgagtct acgatttgcg tacgatcaca
    56461 actacacaca aagtggcgtt gaaagatgga ggtgcaagct aatgtggaaa acgattctgc
    56521 gacgtgtttt attgatgatc ccgcaagtta ttattttaag tgttctgatc ttcttattag
    56581 cccaagcgat gccgggggat ccatttaccg gtttgatcaa tccaaatcag gatccggcag
    56641 tgatcgaaca gatgcgacaa gcagctggct tgaatgaccc atggtatgtc cagtacttcc
    56701 gttggatcgg aaatgctttg caaggcgatt ttggtcaaag tttcttatac aaacgctctg
    56761 ttgctgactt gatcggcgaa cggatcatca acactttata tctatcgatc ttgacagttg
    56821 tccttactta tttgattgct gtgcctctgg ggatgcttgc tggccggtat caaaattcaa
    56881 tcttggataa gattgtcgta atctacaact tctttagttt tgctgttcct ttatttatct
    56941 tcggcttgat catgctattt gtttttggtt atcgtttagg ctggttccca acaagtggat
    57001 cacaaacact aggtttgcaa ggtggattta tcactcaatg gatagaccgt ttgaaatata
    57061 ttctgttacc atcaattacc caagcatttt tggcgactgc cgtcacaatc caatatctgc
    57121 gaagtgaagt aatcgattcg aaatcgctcg attttgtcag aacagcccgt tccaaagggg
    57181 taccgacgaa taaagtattc agtcgacata ttttccgaaa tgcagcgttg ccgatggctt
    57241 ctcaaatggg gtatgaaatc acttcgttga ttgctggttc tgtagttatc gaaaagattt
    57301 ttggttatcc gggagtcgga aaactattta ttgattccat cggccaacgg gactataccg
    57361 tgatcacatc cttagtattg attttaggaa tcgcgacgct agtggggaca ttactttcag
    57421 atatcatcat gagtatcgtt gacccgcgta ttcggattca ataaaaccaa tcagaaagga
    57481 gaatggatcg atgagtgaaa aaaacaatac agcagttcaa gaaagtattc ctcctatggg
    57541 gatacgaatg attgctcgag aatttaaaaa agataaaatt gctatttttt cattaggttt
    57601 gctagtggtt ttattattag tgatctttat cggtgcgatg ctgaccgatc aagacaaagt
    57661 gatgtatgtt agtatcttcg acaaatacgc tgcaccagga acagtttcga cacaaggaca
    57721 aaagttcatt ctgggagcag atgaaggcgg acgtgatgta ttaggacaat tgatcatagg
    57781 tgctagaaac tctgttacga tcggatttgc gatcacgatc cttacttcga tcatcggggt
    57841 tggattaggg atcatctctg gctactacgg agggatcatt gacaacatct tgatgcggat
    57901 cgttgatttt atcatgattc taccgatcat gctgatcatt atcgtcttcg tatcgattat
    57961 ttccagatac aatgtctggt cctttatatt catcatgagt gcattttatt gggtagctaa
    58021 agcccgatta ttccgaagca agacattatc tgaagcaaga agagattatg tcagtgcctc
    58081 aaaaacaatg ggaaccaatg attttaaaat catgttccgc gaaatcatgc cgaatctaag
    58141 ttcattaatc attactaatt tgacgatgaa ctttgcggca aacatcggta tcgagacaac
    58201 tttgactttc ttaggtttcg gcttgccggc aaatgtacct agtttaggta cattgatcgg
    58261 ttatgcaagt aacggagaag tcttagtgaa tcggcaatgg atctggttac ccgcatcaat
    58321 ccttatttta gttttgatgt tgagtatcaa ttatgttgga caagcattca agcgttctgc
    58381 agatgcgcgc caacggttag gctagtatag aagcgaaggg ggaaatagga atgaagaaaa
    58441 cgatttttgg atttgcagcg ctgatgtctg ctgtcgttct tgcagcatgc gggggcggag
    58501 gtacagatac ctctggaaac aactctggag gcaacggcaa taacaaagag gaattaactt
    58561 ttccaattgc aacaaaaaat aatgatgatg caatcgaaga cggcgaatta gaagctgcgg
    58621 tcgcaacgga cagtcagttt aaaggattgt tccagtggga gttttaccaa gattcatatg
    58681 atgcagcttt tatggctcct tctcatgaac cgctatttgc aaacgatgac gatttcagaa
    58741 tcacagacga cggagctgca acgcttgatc tagatgaaga tacgaaaaaa gcgacaatca
    58801 aagtaaaaga caacgtcaaa tggtcagatg gtgaagatgt aacggcggac gatgtgattt
    58861 tcccttacga aatcattggg aataaagatt acactggtat ccgttatgac gacactttcc
    58921 gtaacatcgt aggtatggaa gaatatcatg atggaagtgc cgacacaatc tccggaatca
    58981 aaaaaataga cgacaaaaca gttgaaatcg aatataaaga agtaaaccca agcatgcttc
    59041 aatcgggtgg tggtatctgg tcatatgcag caccaaaaca tacgttagaa ggtgtagcaa
    59101 tcaaagatat ggaatcaagc gatcaagtac gtaaaaatcc agtgactttt ggcccttatt
    59161 acatgagtaa tatcgttgca ggagaatcag tagaatactt gccaaacgag cactactgga
    59221 atggtacgcc taaattgaaa aagatcacga tgaaatctgt accgatggca agtgcaactg
    59281 aagcattgaa ctctaaatta tatgatatga ttttccaaat gccaacagat acttacgata
    59341 cctataaaga aattgctggc tatacaaact taggccgtga acaaacttct tatacttatc
    59401 taggattcaa gttaggtaaa tggaatgcag atacaaagag tgtggaatac aatccagatt
    59461 caaaaatggc tgataaatca ttacgtcaag caatgggcta tgcattagat aataatgcag
    59521 taggtgaacg tttctatgca ggtttgagaa gcaatgcgac ttcattgatt cctccagtat
    59581 tccagtcata ccatgatgat gagatgaaag gattcacaca agatctagac aaagcgaaaa
    59641 aactattaga tgatgctggc tacaaagatg tggatgacga tggtttaaga gaagacaaag
    59701 atggaaataa actaacaatc aactttgctt cgatgtccgg tggggaaaca gcgcaaccat
    59761 tggcagatta ctatatccaa caatggaaag aaattggttt ggatgtccaa tacacaacag
    59821 gtcgtctgat cgaattcaat tctttctatg ataaattaga aaacgatgat cctgaaattg
    59881 atgtttacca agctgcttgg aatacaggaa ctgatccaaa tccaagtgga ctatggggac
    59941 caaatgctgc gttcaactac actcgttatg aatcagaaga aaatacaaaa ttgatcaacg
    60001 atatcagttc aagcaaagca tttgatgaag agtaccaaaa agaagcatat caaaaatggc
    60061 aacaatatgc atttgaagaa gcatttgcga ttccaacgtt gttccgtaat gaagtattgc
    60121 cagtcaacga ccgtgtcaaa gactgggatt ggtcttatga tgtaccaaac ccatgggcaa
    60181 atgtttctgt tacagcagat tcacgttcat aatcactaaa aatgaccgaa aaagggcgat
    60241 gtcctttttc ggttttttga tatttttctg tttatttctc taatattcag gtcaaggcgc
    60301 tgatttcatg acttctccaa taaacggtgt tcaaagtcca gtttctcggt attaagtcaa
    60361 atttatgcaa aaatatggaa agccattttc acaaaatttt ttcttcatac tcgaaactaa
    60421 acgaattttt cacaacttct cgtcaatcac taggattatt aaagctttac ttaccttttg
    60481 accgaaccat ttcaaatttg acaaaaaacc agtataataa ggaagtatcg aaaaaatatc
    60541 cataaaagga atgtggaaaa tggacaacca gctgacaaaa gaactaaaag aaaactataa
    60601 catcgttttt cacgatctaa atttgcttga acaagctttt acccattcat cctatgtgaa
    60661 tgagcatcgc aatctgcaat tatcggataa tgagcgactt gaatttttag gagatgccgt
    60721 tttagaactg atggtttctc agtatttatt ccgtttatat cctcatttgc cagaaggaaa
    60781 attgacaaaa acacgagctg cgatcgttcg cgaagacagc ttgtcgaaat ttgccaaaga
    60841 atgtcacttt gatcaatatg ttttgttggg caagggagaa gagaattcag gaggacgtac
    60901 gcgaccagca ttgctttgcg acctattcga agctttttta ggcgcgttat atcttgacca
    60961 gggattcgac acgacccatg cattcatcga aaaagtgatt tttcctaaag tcaaagcagg
    61021 tgctttttca catgagatgg atcataaaac aaaactacaa gaagtcttgc aaaaatcagg
    61081 tgatgtatca atcgaatacc gattgatcaa tgaagaaggt ccagctcatg aacgagtatt
    61141 ttggatcgaa gtttatgtag atgatcaatt gattggcact ggacaaggga aatcgaaaaa
    61201 attagcagaa caagctgcgg cggaaaatgc attggcagca ctgtaaaagt aagtgaggga
    61261 aagctttgta tttgaaacga attgaaatag ccggttttaa atcatttgca gacaagacag
    61321 tcattgattt tgaaaatagc gtcacagcag tggttggacc aaatggcagc ggaaaaagca
    61381 atattacaga ggctatccgc tgggtattag gagaacaatc tgctaaaagt ctgcgaggcg
    61441 gaaaaatgcc ggatatcatc tttgccggtt ccgatacccg taagcagctg aatattgcag
    61501 aagtcacagt gattttagac aatagtgatc attatctgcc attggagtac aatgagatca
    61561 gtgtaacccg ccgttatcga cggactgggg aaagcgagtt tttcatcaat aaacaaagct
    61621 gcagattaaa agatatccaa gaattgttct tagattctgg attaggaaaa gaatcgttct
    61681 caatcatttc tcaaggaaaa gtagaagcga ttttcagtag taaaccggaa gaccgtcgtg
    61741 ggatctttga agaagcagct ggtgtcttga aatacaagca gcggaaaaag aaagcagaac
    61801 agaagctttt tgaaacagaa gacaacctca gccgcgtcca agacatcatc cacgaattgg
    61861 aagaacagtt aactcctttg gccgcccaga gtgaggcagc aaaggagttt ttacggctaa
    61921 aagaaacact gactcaaacc gatgtctctt tgatggttgc tgaaatcaag acagcaaaaa
    61981 aagattggga caacaaacaa gcacagcttg cgaaattcaa tctagaatta ggcaaactat
    62041 ctgagtcgat ccaagaacaa gaatccattt tggcgaagca gcgtaaagaa aatgcgcaag
    62101 ccgatcgtct gatcgaaaaa aatcagcaag tattattaga tctttcggaa aaactgaagc
    62161 agacagaagg acaaaaagat gttttgcagg agcggacaaa gcatacacaa aaaagctcgc
    62221 aagaatatca aacttcatta gcagaagcac aaaaaaaggt taaacatttt gaaaagcttc
    62281 aagaaagctt gatgaaagca gcagcagaaa aagaaacaga gatccaaaaa gctgaagcaa
    62341 atctcataaa aacacagcaa gaattagaaa aataccaaaa atcaacgaaa gaattattgg
    62401 cagaattaag agatcaatat gttgatttga tgcaagaaca agcagctgta ggaaacgaac
    62461 tgaagtatct agaacgtcaa tatatacaag aaacagcaaa aagcaagcag acgttagcca
    62521 agcaatcgga agtagaagca tcagtagacc gattgatctt acaaaaagaa gaattgacac
    62581 aaaagcaggc gcagctaaaa tcatctttaa ctgaaacaaa agagaagtta gaaatgatcc
    62641 aacaaaacgg gaaaaagttc caagaagcat tagcaaaaga acagcctaaa atgtatcaat
    62701 taatgaatca agtccagcag ctacgtgctc gtaagaaaag ccttcaagaa atccaagaaa
    62761 actattttgg tttttaccaa ggggttcgat tggtcttgca gcataaacag cagctttcgg
    62821 gtattgtcgg agcagtggct gaactgatcg atgttcctgc agatttcacg ttagctatcg
    62881 aaacagcttt aggtggggca gcccagcatg tcatcgttga aaatgaaaaa gatgctcggc
    62941 aagccatcac gtatttgaaa cagcaaagag gaggaagagc aacattcctt ccactgacga
    63001 caatcaaacc aagacaatta ccggctcata tattgacaca agcagcagct gtagaagggt
    63061 ttatagggat tgccagtgaa caagtcagtt atcccgatca aatccaaact gtggttcaaa
    63121 acctgctagg aacgatcctt ttagccaaag acttaactag tgccaatgca atcgcgcaaa
    63181 cgatccgcta tcaatatcga gtggtttcct tagaaggtga tgtgatgaat gccggaggat
    63241 ccatgacagg tggagcgaat aaacgaggaa accaaggcag tttgtttgta caaaatcaag
    63301 aattgaaaca gttgactagt gaatttgaag aagcagataa acaactccaa gcacaagaaa
    63361 aaaaagttca ggagctgcaa caagaaacag ctcgtttagc cgaagaacaa gaagttttgc
    63421 gtacacgagg cgaacaatta cgttttgaag aacaagaagc gacaaatcag ctacaaaaca
    63481 tcataaatga acttgaacgt tttgaaaaag aaaagcaaat ctcaactttt gagacacgtg
    63541 aactgcagca gtttatcgaa gattacgaaa agcaaacgaa cgaattgaaa gacaaacaaa
    63601 cagatcttga atcgcaacga caaaaaatcg acgaagaaat caaatcgtta agtcaagaaa
    63661 gcgaccagat ggaagccaga agagcacaag tacaaagtca aaaagcacaa gaacaagcag
    63721 atcttgctgt cttgaaagag cagtttaatc atctgcagat ccagctccgc ggtgcccgag
    63781 tccaaaaagc agaggcaacc gaacgtcaag aagcgataga aaagcagttg gcgacattga
    63841 cagcagactt ttctgatcat gaagtcacag aagaaagttt ggaaaaacaa attaacgaat
    63901 tatctgctca gcgagaaaca ttaaaagccg agttggcaaa agccaaagag cagcgagata
    63961 gaaggcaaaa ggagatcgat aagcttgaag cagtcttggc agagcgtaat cgtgagcaaa
    64021 aagcacgact gtcggaacaa tccaaattag aagtccaaaa agaccgagct gaaatgattt
    64081 tagataatca tttgacctat ctgcagtcag aatatcagct gacatttgag aaagcttccc
    64141 aagactatca agaaacaacc gatatcgaag acagtagaac aaaggtttca tccttaaaag
    64201 aacagattga aaaactggga ccagtaaact taaattcaat cgaacaatac gaacaagtca
    64261 gtgaaagaca tacattctta gcaacacagc gagatgatct gttagcagcg aaaaaccaat
    64321 tgtttgaaac gatggatgag atggatgatg aagtgcggac aagattcaaa gaagtatttg
    64381 aagctatccg tcaagaattc aaaattgtct ttccaaatat gtttggcggc ggtaaagctg
    64441 aattagtact gacggaacca actgatttgc tgaaaacagg tattgagatt gaagtacaac
    64501 ctcctggcaa aaaactgcaa agtctaagtt tgctttctgg aggagaacga gcacttacag
    64561 ccatcgcgtt gctcttttcc attatccgtg tctgtcctgt tccgttttgt atcttagatg
    64621 aggtagaagc agcgttagat gaagccaacg tgaaacgttt tggtcgttac ttgagcgatt
    64681 tccaagatga cacacaattt atcgtagtca cacaccgcaa aggaacgatg gtcgcagcag
    64741 atgtactgta cggagtgacg atgcaggaat ccggtgtatc aaaaatcgtt tctgtacgga
    64801 tggaagatat caatgaagag gcgaaaatca acgaaacgaa agtgtaggaa cgaaaatgat
    64861 caaattagta gcaatcgatt tggacggtac attattaaat gaacaaaagc tgatttcaga
    64921 tgagaataaa caagcattag cacaagcaaa acagcaaggg gtcaagatcg ttctatgtac
    64981 aggacgcccc ttagctgcga tggcgcatta tctgcaagaa ttaggactgg tagatgaagg
    65041 tgactacagc atcactttca acggcggttt ggtacaaaaa aatgatactg gtgaaatcat
    65101 ggaaaagaaa gtgatggaag tatcagatat ccaacgctta tacaaactgg ctcagcaatt
    65161 ggatctgcca ctagatgttt tgtctgacaa tgtggtttta cagctgccta gtgccccaag
    65221 gaagcaatcc ttgtacaatg tgttgaataa gctgctgcaa ttccaaccag cggatataag
    65281 cgatattaca gatgaatttg tgcttaataa ggcagtaatc gcttatgacc aagaagaatt
    65341 agatcctaaa atcaaagaaa taccagcaga attccatgaa atgtatgaga tcatcaaaac
    65401 aagaagtatg cttttggaat ttatgccaaa aggtgttaca aaagcttatg gtatttcttt
    65461 attggcaaaa gatcttggtt tgaaacaaga agaaatcatg gcgatcggtg atgaggaaaa
    65521 tgatttaccg atgatccaat atgctggact aggcgtagcg atggagaatg ctgtgccttt
    65581 cgtcaaagaa gcagcagatt tcgtgacagc cagcaatgaa gaaaatggcg tagccaaagt
    65641 gatccaaaaa tttgtattag agtaggggga gtcaaatggg attttttgat aaaatcaagc
    65701 gtgcttttac tggcgaagag aaaacagaag aagttgaagt acaggagaag tacgacaagg
    65761 gtttagaaaa gacgcgtaaa acatttggtg aacggatgaa tgagttgttt gccaattttc
    65821 gaacagtcga tgaagacttt tttgaagaat tagaagaaac acttatcggt gcagatgtag
    65881 gttttgaaac agcgatacga attaccgaag cattacgtca agaagtgaaa ctcagaaatg
    65941 cgaaaaaatc ggcagaagtc caaaatgcga tcatcgagaa acttgtggat ctctatgaag
    66001 cggaaggaat caatgaagta aatgaactaa atatccaaga agacggacta acagttattt
    66061 tgtttgtcgg tgttaatggt gtagggaaaa caacaagtat tggtaaactt gcccatcagt
    66121 tcaaaaacga aggtaaaaaa gtattgatgg cagcagcaga taccttccgc gctggagcaa
    66181 tcgaccagtt agttgtctgg ggcgaacgcg ctggtgttga agttgttcgt ggcaatgcag
    66241 gaggcgatcc agcagctgtc gtattcgatg ctttggaaag agcaaagaca gagcaggcag
    66301 atgttttact agttgatact gcaggccgac tgcaaaataa agtgaacttg atgaacgaat
    66361 tagaaaaaat aaaacgagta atccagcggg aattaccaga agcaccccaa gaaacacttc
    66421 ttgtattaga tgcaaccact ggtcaaaacg cgatggttca agcaaaacaa ttcaaagaaa
    66481 caacagatgt gaccggtctt gttttgacaa aattagatgg tacagcaaag ggcggtatcg
    66541 tacttgctat ccgtaatgaa ttgcatttac cagtgaaatt ggtcggtctt ggtgaaggaa
    66601 tcgatgactt ggagccgttt gatccaaatg attttgtcgt tggattgttc aaaggattac
    66661 taaaagaaga ataacccatg tctaaaaagc aggagctgtg acaaaagttg cagctccttg
    66721 tttgaatgat tgtttttaac atagctgtgt ttttgatgcc ttcattttta tacttatttg
    66781 taagcggttc tcaaacaatt tcgttctttt tcttagactt tttgattata attagattca
    66841 tatgaattgg aggtcactta atgatgaatt ttaagcaatt attattgcat gtcaatgcgc
    66901 gcccttttat cgaccaagct cgatttggta tagaacggga aggacagcgg gttgatcttg
    66961 caggaaatct agcaaaaacc gatcatccag caatctttgg cgatcgatcc tatcacccct
    67021 atatccaaac agattttagc gaaacacaaa cagagatgat cacccctgtt accgattcta
    67081 ttcccgaatt atttcagtat ctcgctgctg tttatgatgt gactgctcgt tctataccga
    67141 aagaagagat gatctggcca ttaagtatgc cacctgcctt accggaaaaa gacgaagaga
    67201 tcattattgc aaaattaaaa aatttcgaag atgtcttgta tcgacgttat ttagcaaaag
    67261 aatacgggaa gcgaaaacag atggtaagcg gtattcattt caattttgaa tttggcgatg
    67321 agttgctaag aacattgttc agccatcagg aagaatttca agatttttcc gaattcaaaa
    67381 cagaacttta tttgaaaaca gcgagaaact ttatgcgcta tcgctggatg atcacttatt
    67441 tattcggtgc ttcaccaatg agtgagaaga actacttttt agatgaatca catccgcaag
    67501 aacctgttcg cagtattcgc aacagtgcgc tgggctatac gaatcatcca aatgtgaaag
    67561 tttcctatgc ttctatgaaa cagtatttag cagatatcga gcgaatgatc gaagaaggga
    67621 aactttcgga ggagaaagaa ttttatacac cgcttcgttt ccgaggcgga aagaaagtcg
    67681 cagatttagc aacaacaggt gttcgttata ttgaattgcg gaatatcgat ttgaatcctt
    67741 atgcaagatt gggtatcaat ccagagcaag ttcggttctt acaattattc ctgatgtaca
    67801 tgttatggac agaagaaaaa gaagactgtg accaatgggt agcagaaggt acgactcgaa
    67861 ataacaaagt agcactagaa caaccatcag atcaaacaga atttcatcaa gaaggtagag
    67921 aaattcttga aggaatgaag caaatgctgg ttgaattgga ttggttggat tctctttatt
    67981 tagtagagga agcattgact caaatggatc atcctgaaca aacgttagca gcgaaactct
    68041 atcaagaagc acagctatcg agtcagcaag aagttgctgt ggcattggga catcaatatt
    68101 ataaagaaag ccatgaacgt ccctatcagt tggccggttt tcgcgaaatg gaactttcta
    68161 ctcagatatt tatgttcgat gccatccaaa aaggtgtcca agtcaaagta ttagatgagt
    68221 cggatcaatt tttgcgtctg caattccaag accatgtaga atatgtgaaa aatgcaaaca
    68281 tgacaagtaa ggacagctat atcgtcccac ttatcatgga aaacaaaaca gtaacgaaaa
    68341 aagtcttaaa agaagctggg ttccgggtac caggaggcgc agaattttca tccatggaag
    68401 aagcggtaaa agcttatccg agatttgccg agcaggcatt tgtgatcaaa cctaaatcaa
    68461 ccaattacgg gttaggtatc acgattttca aggaaggagc tagtctggaa gattatcaag
    68521 caggtttagc cattgctttt cgtgaagaca gttcggtttt agtagaagaa tttatgccgg
    68581 gaacggaata tcgtttcttt gtgatcgacg gagaagtcca agccatcatg ttgcgagtcc
    68641 ctgctaatgt tattggtgac agtatccgaa cagtgaaaga acttgttgaa gaaaaaaata
    68701 gtgacccttt gcgtggaacg aatcatcgtg cacctttaga attgatccaa ttaggtgaat
    68761 tagaacaatt gatgttgaaa gaacaagggt tgacgatcga atcagttccc caagccaatc
    68821 aaattgttta tctaagggag aactcaaata tcagtacagg tggtgattcg atcgatatga
    68881 ctgatgaatt ttctgaagct tacaaaaaaa tcgccgtttc tgcggtagaa gcactaggag
    68941 ctaagattag tggtatcgat ttgattatac cagataaaga aatcgatcct acaactgata
    69001 aaaaagcata tgggatcatc gaagcaaatt tcaatccagc catgcatatg catgtttatc
    69061 cttttgcagg aaaaggcaga agattgacga tgaatgtatt gaaattgtta tatcctgaag
    69121 tattttaaaa gaaaaagctt ttgacagatt gtcctatttg atgtcaatct gatcaaaagc
    69181 tttttttgca tgtaaaaatt tatgtgtttt ttcgttttaa aataaacgat cccagaagct
    69241 ttttttcttt tttggttttt ctttttcagc tggcgaagag ttttcttctg ggtatttttt
    69301 ttcgatatca atcacaggtt catttactgc ttcttttgct gttaagagca atccgaattc
    69361 ctcaggattt gcagtgacgt aatcgttgac tacagtgaaa cggctattgt ttttcgttgc
    69421 tagctggata tattggtttt ggacagaaag gggtagagcc ccattcaaga gtatggaagc
    69481 atctggatgt tttttgagtt cttttaaaaa gttttctttg ttttttggtt ggatcatttc
    69541 agcaacagtc atactaagat agcagcgttc cctgaatgtc cctaaatatt tatgctgttc
    69601 ttcagggttc actaaaggtg taccataaag tcccttatcc aaatggcttt ttacatcatc
    69661 ttgtgccata aaaaataccc tccttcttct ctttgtcttt cttacgatta tagcatattc
    69721 ccaataatac ctaaaacata tgaaaaagcg gttgcttttg ttcaacgatt cacttaaaat
    69781 aagaatgaaa atcattctca attagaaagg tggattggat gcgctacgga cgactagtag
    69841 cattattagt tattttaagt agtatttcat tgttcatcgg agtatcgaat gtacaattgt
    69901 cagaactgct ccagttagat gggcaaagct tacaaatctt gctagtgact cgtttgccac
    69961 gaacgatttg tttgatcctt gtgggagcga ccagtagtat ttgtgggttg atcatgcaac
    70021 atttgaccca aaataaattt gtttcaccaa cgactgcggg cacgatggat agtgcgcgtt
    70081 taggtatttt agtggctatg attttcctac ctggtgcatc cttgattgtc cgatccttga
    70141 cagcattttg ctttgctttt gcaggaacat tgcttttttt atcccttacg cgattgttcc
    70201 tccagaaaaa tcaattgatg cttccgttga ttggagtcat gttagggaat atcattggtt
    70261 cagttgctac tttttttgct tatcaattcc agttggtaca aaatatgact tcatggcttc
    70321 aagggaattt tgcgactgtg atacgaggaa attatgagtt attgtatgct gttgttccat
    70381 tgttgctctt ggtttggcta tttgcttacc gatttacgat cattggtctg ggcgaatcgt
    70441 ttgcagtaaa tctgggaata gattaccaga agatgcagtt tttaggcatt ggcttagtat
    70501 cattggctag tgcagtcatg ttgatcatgg taggaagcat tccttttatc ggagtgatca
    70561 ttccgaatct tgtctctaga atatatggag atcaagtgca caaaacaatt ggcgtaactg
    70621 ctttagcagg tagtttgttc ttagtatttt gcgatcttgt cgctcgcgtg ttgatttttc
    70681 cttatgaaat accagtcagt gtgatcgttg gaatcatcgg tggggctatc ttcctttatc
    70741 ttctgataag gggacgacga tatgcttaaa aaaaagccat ctatttttat tggtatgctt
    70801 gttcttgttt tatttgttac aggaatgtac ttgacgatcc atacatatgg aaactggcat
    70861 tttgcatgtg cattgaggag taagaaaatt tttgcattta tccttgtctc gctagctgtc
    70921 agcagtgcca caatcagctt tcagacaatt acgcataacc agtttttgac tccttcagta
    70981 ttgggacttg agtcgttata tgtccttgtc caaacggtcc ttttcttctt tatcggagga
    71041 gtgacgatgc ttcagcagac aggtacagtt tttttcttat tgaacgttgg gctgatggtc
    71101 gggctgagtc tattgttatt tttctttctt ttgaaaaaaa tgagagcgaa tcttttttta
    71161 ttactgatgt cagggatgat tttaggtacg ctttttggta gtgtcagtac ctttttacaa
    71221 gtcatcatgg atccgaatga atatgatttg cttcaaggca gattatttgc aagctttggc
    71281 aatatccaga gtcagtatct tttgcctgct tctttgatca ttatcggctg ctggtacctg
    71341 ttatttcgat atcgacatgt gctagacgtt ttccatctcg gaaatgaaca agcagtaaac
    71401 ctcgggatag acacagatcg ttttgaattt ttgattttat gttgtgtcag tcttttgaca
    71461 gggacatcaa cggctcttgt gggtccagtt acttttttag ggtttattgt cgcaaatatc
    71521 agttatcgtg tcttttctac ttatcgtcat ggtcagctat ttatcggaag ctttctggta
    71581 gctatgctgt ttctgacagg aggacagctc gcagtagagc agattttcgg ctggaataca
    71641 acagtgagtg tcgtcgttga atttgtcgga ggaatttatt ttgtcacaaa attgattcgc
    71701 gaaaggaaga gtatcctatg attgatttac aaaaggtcaa taaactgtac catggaaaat
    71761 cagtggttaa agatgtacag ttaacgattc ctgaaaagaa attgactgct tttatcggtc
    71821 caaatggtgc tggaaagtca acggtgttgt cgatgatcag tcgtttgata ccaaaagata
    71881 caggtgaaat ctatttagac cataacgaag taaaggcttg gaaatccaat gaattagcca
    71941 aaaaattatc gatcctacga caatcgaatg aaataaatgt ccaaatcacc gtgaaagaat
    72001 tagtagaatt tggacgattt ccttatacga aaggaaaatt gacgacaaaa gacgaggaaa
    72061 tcgttcagga atcactggaa caccttgggt tagaagaaat agcggatcaa tcgattgaca
    72121 ctctttcagg cggtcaactc caacgtgcgt atattgcaat ggtgctagcg caagacacgg
    72181 attatatttt actggatgaa cctttgaaca atctagatat gaattttgcg gttcaaatca
    72241 tgcagatttt aaaaaattta gtacaagaaa tcgggaaaac aattgttatt gttcttcatg
    72301 atatcaactt tgctgcaagt tatgcagacg aaattgttgc aatgaaagat ggacagttat
    72361 ttacgcaagg aacgacccaa gaaatgataa aaaaagatgt attggatgaa ttatacgaaa
    72421 tggacatacg gatttgtgag attgaaggcc gcagattctg tctttatttc taaaactaac
    72481 attaggagag aagagatgaa aaaattagta gtaggtatct tgattctagc atcatttggt
    72541 ttagcagcat gtggaaatag caacaacacc agtcaggcag acacaaaaaa aagcagcaca
    72601 caaactgaaa ctacattgac gataacagat agtaatggag accaaatcga ggtgccaaat
    72661 aatccagaaa aagtcgtcgt attcgacaat ggttctttag atacgatgga tgctcttggt
    72721 gttggagata aggtagttgg agcagctaca agcagccttc ctgagtatct ttcatcctat
    72781 aaaaaagtag aatcagcagg tggaataaaa gaaccagact tagaaaaaat taatcagctt
    72841 cagccagatc taatcatcat ttctggccga caaagagatt ttcaaaaaga tctaagtcaa
    72901 attgctccga ccattttcct ttctttagat gcgaaaaatc catgggaatc tttccagcaa
    72961 aatgtcactg ctttagggga gatctttggt aagcaagaag aagctaaaac tcaattagag
    73021 gaattatcta gtgcaatcga tcagacgaaa aagaaagcag aagctactga caaaaaagca
    73081 ctagtcacac ttgtgaatga agggcaatta tcagcctacg gatctggttc tagattcggt
    73141 ttcatccatg atttatttgg ctttgaacaa gcagatgatc aaatcgaagc atccactcac
    73201 ggtcaaagtg tttcttatga atatgtgctg gaaaaaaatc cggatatctt atttgtagta
    73261 gacagaacaa aagctattgg tggagacgac agcaaagatg atatttctgc caatgaactg
    73321 gtagcacaaa cgaatgctgg taaaaatcag cagatcatct cccttgaacc agatgtctgg
    73381 tatttaagcg ggggcggttt agaatcaatg aaactgatga tcgaagatgt gaatcaagca
    73441 tttaaatagc ataaataaca gaggattaaa ctctcatact atgtaacaat aatgtttctt
    73501 acattcttaa atagcaagag cctatcctca aagaagttat caaaaaccat ccgtaaaatc
    73561 gtattgatga ttttacggat ggtttttatt tatttgagaa atcttttttc aacgtgaatt
    73621 cattatctac atcatacggg ttgattctag aatggggagt ggcaaaaaat agtgtgatcc
    73681 attttttcag catcaagatc gcctcctttg atttgaatca aaggtacaaa gcgaaactta
    73741 gataaaactt aaatttagat atttttattt tgtaccgatc tcttctttaa acgatgttcc
    73801 atctgctgct tctcggcaat aagtccacta gactagaaaa tatgagaagc tattttctgt
    73861 ccagcgttct tattactcaa agcaaatcgc agttgtcaca atctcttcta aacaatgttc
    73921 aatcagctga ccttcggtat tagctcaaag cactgatttc acaacctcta tttaggcatg
    73981 tgtctcagca agcggttgat agttgatgat ctgcattttt cgataaaaat cggcgggaag
    74041 cttactccac cgttttaata attgatagaa ataactctct tctgctttga ttttatactc
    74101 gcttaatatc aatccaaaac tattcgtttt ttttgaagct aattttttat aaatatccaa
    74161 tgctttggtt ttatcttcat tgacaaagag tgtttttact tcaatataag aaccctcgat
    74221 attttgtgtt tcttcgataa aatagatgtt ttttatatat tccttcatag tgattcctcc
    74281 aaagaagtct tttatttgat aagtttactc taccgaagag ttgttaataa tccgtgaaca
    74341 aaaagtaatt ctttttccaa tagttgttaa aggtttttta ttttttatag tattttttgt
    74401 tatttaagaa aggaataagg ttaaacaaat aacaggttgc tttttgggtc tttttccgtt
    74461 caagaaactg aacgaagaaa agggaaagtt gtctcttttg tgataagaga aatcataata
    74521 aacgaatgac ttactttcca ataaattgag aacagtcaga accagacaaa ttagttgcat
    74581 ctgatgaata aagtgattat tctatgacat gaggtgatac agatgaaatt agaagtgaca
    74641 aaagaagcac aagaagtatt gaagaataaa ttagaagcag acgatcaact tttattagat
    74701 atcgaaaacg gtgacggtcc atttgcagaa agccaagtca gttgccaatt ggatacggca
    74761 tttcgattga tcattgtcaa aaaagataca caaacagatc taagtcttta ttctgtagtg
    74821 gctgatacac cagttggacc aatcaaaatc aaagacagtg caatacttta catggatgat
    74881 ccatccacac ttgctttaga accaacttat aatagtttac aattaaaagg accaagcggc
    74941 ttgagaaaag gcaacttgca agttgttcga aaagactaga gggataaaat gggaaaattg
    75001 gcgatcatca gcgacctaca tgtggacatc aataaattcg gcgaatcaga gctgatgcag
    75061 ctttggcaag ttttgcaaaa gcacaagatc actcggcttc atctagccgg agatacggct
    75121 aataaagtag accgttgcct agctgttgtc gaatttttcg tcaataaagg attatctacg
    75181 acatttcatt tcggcaatca cgagttggca gatgtgccca atgaacagat gatggaccat
    75241 tttccagacc cacatttttt aaatgaacgg tatattgctt taaatgagca gaccgttctt
    75301 ttagggatga atggctggta cgactatcag ttttccgaat tgaaagatta cgaggcaatc
    75361 gtacgaatga agcgtttgta ttggtatgat cggttcatcc agcgtgggaa aacagatctc
    75421 gaggtcaatg cagaaatgct tgcagctgca aaaaatttgc tagaccagct ggaagcaaaa
    75481 aaacttgagg tgattttatc cactcatttc gttcctaaac gggcatttat catgtatcaa
    75541 tctgctcctt atgagcgttg gaacaaatta aatgcatttt taggatcaga atcattcgga
    75601 gaattacttg accgttattc aaatatcaaa caagtagttt ttggccatac gcatcgtcgc
    75661 tttgaagata aatccatcgg tcacacgatt tacagctgca gaccttttgg ttattattat
    75721 gaatggcagc tgacccgaga ttttgtcttg aaagaacagt tgctagacag ctatgatcct
    75781 atgaaattac gtgtcctctt gcgtgcccat cacccagctt ttcaggccta tcaaacacaa
    75841 catttgcaag aggaatttga acaagcgatg acaatcgttc cgtattgaga cagcattagt
    75901 aatgtgtaaa agaaaatacc ttgtaaaaca taaggataag taagagcagt agatccattt
    75961 atcgtggata tgctgctttt tttgcggaaa ataatcagcg aatgtttatt tgatcggatg
    76021 atttgtaact cgcagagtac tagatgcaag cgtgtattct ttttttgaac gataatcaca
    76081 aaaaaaaata taatatacat tttttattta attataatta atattgattg aagtctaggc
    76141 agtcaagtca cattttgata gataggaggg ggacttacga attattttaa aggctatgac
    76201 agctgtattt ctagattgtg tttgcatcaa gttgcaataa aaactagaaa tcaaatcggg
    76261 ctgataatca tttataacta atcgatacat aggaccaaat ataggtctat gttcttttat
    76321 ttcataagta tcgtaaaaca gaattttgct atggattaat ccaatcgact gatatgttta
    76381 gtaaataaag aaagaggaat gtcaatgatg aaaaaagaaa tcgtattaac agcagcaatg
    76441 atgttattca gcatggtagc aagtacgact tttgtttctg caactgaagt atacccaaag
    76501 gaatataaca cagaaggaac gattacattt gaagcaggag atgagggtgt aactcctcct
    76561 gttgatccag agaatcctga tcccaataaa cctgtcgatc catcagatcc tccaagccct
    76621 ggaacaggag gggcattatc gattgattat ggatcaaaat tcaaatttgg gactcaaaaa
    76681 atttctactg cggataaaac gtattatgca gctgctgatg tgatgaacga tgggtctaga
    76741 aaaccgacat atgtccaagt gacagatagg agaagtacct tatctgggtg gaaattaagt
    76801 gtctctcaac cagaacagtt taaaactgcc agtggggatg aactagttgg agcacagctg
    76861 aaatttacga aagggcaggc tgtttctttg gttgatccaa catatacgcc gcaaacggtg
    76921 aattctgaat taacgttgac accgggagga aacaatacgt tagcaattaa tgctaaatca
    76981 ggaacaggtg tggggacttg ggtctatcga tttggtgcaa atgaaaatga aaatcaagat
    77041 gcggtacagc tttcggtacc cggtaaatct gtcaagttag cgcaacaata ttccactaaa
    77101 ttagtttgga cattagaaga tacacctaac aactagttta ctgaaaagaa ctgttaaaaa
    77161 taaatacgta gtcatattgt cttgcgaggt aatcgagcaa aaattgtcaa aggctaggct
    77221 aaatttgttt ggatacgaat agttcatcta ggaatttaac gatcaggagg aaaagatata
    77281 ttgaaaaaga ggaggatcgt gctcatttta agcagtctct tagtgactaa tatctttccg
    77341 atcatagggt actctttgga attgaatcag ttggagcatg gaaatagaag agacgaagaa
    77401 agcaaactca cttcgacatt cgacagttta atagaatcca accagacaca agaaagcagt
    77461 gaggatttaa gtatttcgag tagtggagag accactgaca acgtagaagc tgagggttcg
    77521 gacaaacaag gactagaaga tgcaccaatt tctgcggttg agctgcctga cgatcccaat
    77581 attatttcta tcgataaaat ttttcaaaca cctatcggta ctagcacgtc catattggaa
    77641 aatgggaagt tactgcagtt gaacccagca gaaaggtctc aaagaggagc tatttggtca
    77701 aataagaaga ttagtttact ctcggatttt acatttaaat cttatttata cttaggaaat
    77761 caacgagata gagcgggaga tggcatgacc tttacattga ctggcgatcc tagaatggct
    77821 agtaatccca gtcaagttat tggatcgcca ggtatgggaa ttggctcata ttctactcgt
    77881 tctggccaac cttacattgc gaatgcgctc tcgattgagt ttgatacgta taaaaatagt
    77941 ggttcttctg atcgaatgga tcgagagata actgctgata atagagggca tggacatgtc
    78001 gcttttgtca cgcctaaacc aaataataat aattattctg gagagcatac tggcgtgacg
    78061 gtagctccta catttctttc taatggcaca tggcgcttgt taataattaa atgggacgcg
    78121 gagcaaagaa gtcttactta tgatttggaa ggtgtcggtc actcttctcg gattatacct
    78181 gatctcatta ggcagtttgg ttctacagaa gtaaattggg ggtttacttc ttcaacaggt
    78241 actttttttc aagaaaatgc tgtcgctgtc acgcgacttc catcgaatgt cacatcaaca
    78301 gctgatgtaa aagtcaacga tagtgattat ggggccatcg ctgaagctgg aggaaacgat
    78361 aagataacta tcagaaatac attgaaggtc gagggggatt ttcttgaata taagaaaatg
    78421 cagactagta tcgacctccc acaagaatta actattcccg agcatatctt gatcaatgga
    78481 aatcaaattg atgccaaaga tatcttgata accggaaatc gagtcacgct aaatctgaca
    78541 gattatttta ttgctggaga tacgttaata atcgaattgg agacaacttt gcaggaagta
    78601 attcctgaaa aagtactcac tatgcatttt gaataccttg aaaacgggaa tttgattgag
    78661 caatcgaatg aaataaacct aaagatcgca aagctgaagg agatagatat ccatgtttat
    78721 tataaagata aagacagtca gattgatatt gctgaaatga aaacaatcac tgggaaaata
    78781 ggagagaagt ataaagaaac tcctattgat attgaagggt atgtttttga ctcggattca
    78841 gcgaatacag aaggcatctt ctccgaaaat acagaggata tctattttta ttacagaaaa
    78901 ggcaaacttt atctctcaac tgctcctcaa tatgtcaatt ttggaaaaca taaaatatcc
    78961 agtagaccat taagtgaatt cggacagttt tcaggtgggt tgaaaattat agatgaacgg
    79021 gcttttggaa gttggaaatt acaattgaaa caagagaaag ttcttacaaa tggagatttt
    79081 gagcttccag aagctctttc ctttgttacc tctgaaaata caacggttat agggacatcc
    79141 gctgttacga ttttcgaaag tgatcaaaag ggtgaaagcg atttgtcctc tcttttagat
    79201 tctacagctc atcgtggaat cagaatagat attcctgtag aaaaccaaag aatcggaacc
    79261 tttgagggga aactatcttg gatacttgca gatgtcccag gaaattaaca ggaagagaag
    79321 aatataacaa aattattgag tttcaagaag ggcacaggta ccataggctc ttgtgtccca
    79381 cagagacaag attttgttat agtcttctct tacatatgat taaaggagcg attttcttga
    79441 gaccaaaaca acaattattt ataacactcc tagttatttt aggggcactt tttaacaata
    79501 tatcagtgtc agctagtgca tttaattttt cagtgacgcc gataacttca gagaatcaaa
    79561 tcgacaagag aaaaacttac ttcgatcttc aacttgatcc ggatcaggaa gtagaagtaa
    79621 aagcggaatt gagaaatgat acagaaaaag aagtgaaaat cgatatttct gtgaatagcg
    79681 caaccaccaa ttcaaatgtg atggttgaat acggaaaaaa cgagattgaa aaagacatga
    79741 gcctgatctt tgatctggta gattatgtga gctatccaca gatagtgacg ctaaaaccta
    79801 aaagtgtcca aaccgttgct tttcatgtca ggatgccgaa tgagcgattt gatggtgtat
    79861 tggctggtgg gatcacattc caagaaatcc agacggaaaa ggaccaaaca gaaactaagg
    79921 atcaaagctt atcgattgag aatgaataca cctatattat tgctttgttg atgcaacaga
    79981 cactgaacga agttgctccg aaccttactc ttcatgaagt taaaccagat caaatcaatg
    80041 caagaaatgt catcttagca aacgttcaaa atgatcaaaa aacctatatc aatcaagtag
    80101 ttattgaaac aaaaattaca aaaaaaggac attctgaagt tttatatcaa gaggaaaaag
    80161 aaggattgca gattgcgccg aatacaaatt tttcttttcc aactgcttta aatggtcaac
    80221 cgctaacccc aggtgaatat caccttacaa tgactgtttt aggaaatgaa aatgaaaatg
    80281 gaaaattttc gagaaaaaaa ggaaatacta cgatcaacta taccaatcaa tgggtatttg
    80341 aaaaagattt tacaatagac ggaaaagtag ccaaggaatt gaacacgacg gatgttacga
    80401 ttaaaaaaga caataattgg atttatatct tagtagcttc acttttctta ttgtttattt
    80461 tattatgcat acttttgttt gttttgagaa aaaaagaaga gaaagaaaag taaggttaag
    80521 ataacaaata gtaaattttt attattgtag gatggttggg ctctttttat aacaatataa
    80581 aatataccat tttattaata aaaatgcata ctagtaaatg atataattct aataacaggt
    80641 ttgatcgcga gaaaggccaa ataaaacgct ctccaaaatg ttaaaaaaaa gacaggacga
    80701 tgtatattat acaaacaaga atcgctgaca acagtgactc ggatccagga aatcagtttt
    80761 atccccagct agttggtgaa tgcatggaaa gtaatatacg tagaaagagc tgatgactaa
    80821 tgaattatga gtttttttta agcaaagaag atcacagaag ttattcatta cttaaatatt
    80881 tagaagcaag ctcaaattta tcagagtcaa tatcgaatgt tcaagaagag ttgtcgctat
    80941 cgacatttct cttgaaaaaa acgatcgata agctgaaaca tgatttacaa aagttcgatc
    81001 tagagaaaaa tttgcgagta accgtcagtg aattggatat atgtctggaa attaatggaa
    81061 aatgttctag caaagctctg ttatctcgtt atataacgga ttctttatct atgaaaatgg
    81121 tattggcttt ctttcaggaa aaatatatct cagtagaaaa ttttgctaaa cagcatcacg
    81181 tgagttattc tgtggcttat aaagtattac aaagactaaa aagaaatcta aaaaaatatc
    81241 aaattttttt tgaaaagaga aagttg
//
LOCUS       DACECY010000003        80429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.003, whole genome shotgun sequence.
ACCESSION   DACECY010000003 DACECY010000000
VERSION     DACECY010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 80429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.003"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(717..3866)
                     /locus_tag="IWO38_000174"
     CDS             complement(717..3866)
                     /locus_tag="IWO38_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ0312297.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3879..5078)
                     /locus_tag="IWO38_000175"
     CDS             complement(3879..5078)
                     /locus_tag="IWO38_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ0312298.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(5068..6663)
                     /locus_tag="IWO38_000176"
     CDS             complement(5068..6663)
                     /locus_tag="IWO38_000176"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ0312299.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(7247..7954)
                     /locus_tag="IWO38_000177"
     CDS             complement(7247..7954)
                     /locus_tag="IWO38_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312300.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(8019..8855)
                     /locus_tag="IWO38_000178"
     CDS             complement(8019..8855)
                     /locus_tag="IWO38_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0312301.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(8873..10282)
                     /locus_tag="IWO38_000179"
     CDS             complement(8873..10282)
                     /locus_tag="IWO38_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0312302.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(10296..12119)
                     /locus_tag="IWO38_000180"
     CDS             complement(10296..12119)
                     /locus_tag="IWO38_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0312303.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(12962..13288)
                     /locus_tag="IWO38_000181"
     CDS             complement(12962..13288)
                     /locus_tag="IWO38_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312304.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(13334..13696)
                     /locus_tag="IWO38_000182"
     CDS             complement(13334..13696)
                     /locus_tag="IWO38_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ0312305.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(13733..13948)
                     /locus_tag="IWO38_000183"
     CDS             complement(13733..13948)
                     /locus_tag="IWO38_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0312306.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            14075..14410
                     /locus_tag="IWO38_000184"
     CDS             14075..14410
                     /locus_tag="IWO38_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0312307.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(14486..16483)
                     /locus_tag="IWO38_000185"
     CDS             complement(14486..16483)
                     /locus_tag="IWO38_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312308.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(16581..16856)
                     /locus_tag="IWO38_000186"
     CDS             complement(16581..16856)
                     /locus_tag="IWO38_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0312309.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            17088..17822
                     /locus_tag="IWO38_000187"
     CDS             17088..17822
                     /locus_tag="IWO38_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0312310.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            17861..19003
                     /locus_tag="IWO38_000188"
     CDS             17861..19003
                     /locus_tag="IWO38_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0312311.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(19098..20489)
                     /gene="sufB"
                     /locus_tag="IWO38_000189"
     CDS             complement(19098..20489)
                     /gene="sufB"
                     /locus_tag="IWO38_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ0312312.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(20494..20964)
                     /locus_tag="IWO38_000190"
     CDS             complement(20494..20964)
                     /locus_tag="IWO38_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ0312313.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(20951..22186)
                     /locus_tag="IWO38_000191"
     CDS             complement(20951..22186)
                     /locus_tag="IWO38_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0312314.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(22183..23469)
                     /gene="sufD"
                     /locus_tag="IWO38_000192"
     CDS             complement(22183..23469)
                     /gene="sufD"
                     /locus_tag="IWO38_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ0312315.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(23482..24255)
                     /gene="sufC"
                     /locus_tag="IWO38_000193"
     CDS             complement(23482..24255)
                     /gene="sufC"
                     /locus_tag="IWO38_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ0312316.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(24452..25300)
                     /locus_tag="IWO38_000194"
     CDS             complement(24452..25300)
                     /locus_tag="IWO38_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0312317.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(25324..26010)
                     /locus_tag="IWO38_000195"
     CDS             complement(25324..26010)
                     /locus_tag="IWO38_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0312318.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(26003..27037)
                     /locus_tag="IWO38_000196"
     CDS             complement(26003..27037)
                     /locus_tag="IWO38_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0312319.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(27432..27770)
                     /locus_tag="IWO38_000197"
     CDS             complement(27432..27770)
                     /locus_tag="IWO38_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ0312320.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(27772..28128)
                     /locus_tag="IWO38_000198"
     CDS             complement(27772..28128)
                     /locus_tag="IWO38_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ0312321.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(28134..29321)
                     /locus_tag="IWO38_000199"
     CDS             complement(28134..29321)
                     /locus_tag="IWO38_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0312322.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(29512..30357)
                     /locus_tag="IWO38_000200"
     CDS             complement(29512..30357)
                     /locus_tag="IWO38_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0312323.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            30591..31556
                     /locus_tag="IWO38_000201"
     CDS             30591..31556
                     /locus_tag="IWO38_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ0312324.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            31933..32976
                     /locus_tag="IWO38_000202"
     CDS             31933..32976
                     /locus_tag="IWO38_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ0312325.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(33038..33502)
                     /locus_tag="IWO38_000203"
     CDS             complement(33038..33502)
                     /locus_tag="IWO38_000203"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ0312326.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            33748..34695
                     /locus_tag="IWO38_000204"
     CDS             33748..34695
                     /locus_tag="IWO38_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0312327.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(34906..36978)
                     /locus_tag="IWO38_000205"
     CDS             complement(34906..36978)
                     /locus_tag="IWO38_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0312328.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(36911..37288)
                     /locus_tag="IWO38_000206"
     CDS             complement(36911..37288)
                     /locus_tag="IWO38_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ0312329.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(37417..37977)
                     /locus_tag="IWO38_000207"
     CDS             complement(37417..37977)
                     /locus_tag="IWO38_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0312330.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            38127..39419
                     /locus_tag="IWO38_000208"
                     /pseudo
     CDS             38127..39419
                     /locus_tag="IWO38_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
     gene            complement(39479..40597)
                     /locus_tag="IWO38_000209"
     CDS             complement(39479..40597)
                     /locus_tag="IWO38_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ0312331.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(40611..42032)
                     /gene="arcD"
                     /locus_tag="IWO38_000210"
     CDS             complement(40611..42032)
                     /gene="arcD"
                     /locus_tag="IWO38_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ0312332.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(42278..43399)
                     /gene="mnmA"
                     /locus_tag="IWO38_000211"
     CDS             complement(42278..43399)
                     /gene="mnmA"
                     /locus_tag="IWO38_000211"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ0312333.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            43803..44402
                     /locus_tag="IWO38_000212"
     CDS             43803..44402
                     /locus_tag="IWO38_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ0312334.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(44540..45826)
                     /gene="efmA"
                     /locus_tag="IWO38_000213"
     CDS             complement(44540..45826)
                     /gene="efmA"
                     /locus_tag="IWO38_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ0312335.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(46097..46441)
                     /locus_tag="IWO38_000214"
     CDS             complement(46097..46441)
                     /locus_tag="IWO38_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ0312336.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(46578..47714)
                     /locus_tag="IWO38_000215"
     CDS             complement(46578..47714)
                     /locus_tag="IWO38_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0312337.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(47903..48877)
                     /locus_tag="IWO38_000216"
     CDS             complement(47903..48877)
                     /locus_tag="IWO38_000216"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ0312338.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(48957..49832)
                     /locus_tag="IWO38_000217"
     CDS             complement(48957..49832)
                     /locus_tag="IWO38_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ0312339.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            49962..51869
                     /locus_tag="IWO38_000218"
     CDS             49962..51869
                     /locus_tag="IWO38_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ0312340.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(52410..55688)
                     /locus_tag="IWO38_000219"
     CDS             complement(52410..55688)
                     /locus_tag="IWO38_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ0312341.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(55959..56504)
                     /locus_tag="IWO38_000220"
     CDS             complement(55959..56504)
                     /locus_tag="IWO38_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0312342.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(56516..57208)
                     /locus_tag="IWO38_000221"
     CDS             complement(56516..57208)
                     /locus_tag="IWO38_000221"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ0312343.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(57227..57511)
                     /locus_tag="IWO38_000222"
     CDS             complement(57227..57511)
                     /locus_tag="IWO38_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312344.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(57524..58087)
                     /locus_tag="IWO38_000223"
     CDS             complement(57524..58087)
                     /locus_tag="IWO38_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ0312345.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            58315..58977
                     /locus_tag="IWO38_000224"
     CDS             58315..58977
                     /locus_tag="IWO38_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ0312346.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            59101..60297
                     /locus_tag="IWO38_000225"
     CDS             59101..60297
                     /locus_tag="IWO38_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ0312347.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(60398..61774)
                     /locus_tag="IWO38_000226"
     CDS             complement(60398..61774)
                     /locus_tag="IWO38_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0312348.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(61811..62677)
                     /locus_tag="IWO38_000227"
     CDS             complement(61811..62677)
                     /locus_tag="IWO38_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ0312349.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(62766..63791)
                     /locus_tag="IWO38_000228"
     CDS             complement(62766..63791)
                     /locus_tag="IWO38_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0312350.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(63809..64483)
                     /locus_tag="IWO38_000229"
     CDS             complement(63809..64483)
                     /locus_tag="IWO38_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0312351.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(64667..65560)
                     /locus_tag="IWO38_000230"
     CDS             complement(64667..65560)
                     /locus_tag="IWO38_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0312352.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(65634..67331)
                     /locus_tag="IWO38_000231"
     CDS             complement(65634..67331)
                     /locus_tag="IWO38_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0312353.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(67324..68637)
                     /locus_tag="IWO38_000232"
     CDS             complement(67324..68637)
                     /locus_tag="IWO38_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0312354.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(68637..72674)
                     /locus_tag="IWO38_000233"
     CDS             complement(68637..72674)
                     /locus_tag="IWO38_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ0312355.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTAINRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(72687..73145)
                     /locus_tag="IWO38_000234"
     CDS             complement(72687..73145)
                     /locus_tag="IWO38_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0312356.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(73443..73961)
                     /locus_tag="IWO38_000235"
     CDS             complement(73443..73961)
                     /locus_tag="IWO38_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ0312357.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(73963..74580)
                     /locus_tag="IWO38_000236"
     CDS             complement(73963..74580)
                     /locus_tag="IWO38_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ0312358.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKFGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(74577..75431)
                     /locus_tag="IWO38_000237"
     CDS             complement(74577..75431)
                     /locus_tag="IWO38_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0312359.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(75428..76339)
                     /locus_tag="IWO38_000238"
     CDS             complement(75428..76339)
                     /locus_tag="IWO38_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0312360.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(76380..77714)
                     /locus_tag="IWO38_000239"
     CDS             complement(76380..77714)
                     /locus_tag="IWO38_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0312361.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            77922..78752
                     /locus_tag="IWO38_000240"
     CDS             77922..78752
                     /locus_tag="IWO38_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ0312362.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(78996..79245)
                     /locus_tag="IWO38_000241"
                     /pseudo
     CDS             complement(78996..79245)
                     /locus_tag="IWO38_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(79436..79630)
                     /locus_tag="IWO38_000242"
     CDS             complement(79436..79630)
                     /locus_tag="IWO38_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312363.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(80135..80320)
                     /locus_tag="IWO38_000243"
     CDS             complement(80135..80320)
                     /locus_tag="IWO38_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312364.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
BASE COUNT        23253 a        17028 c        13994 g        26154 t
ORIGIN      
        1 gactaataca ggtagtcctc ttttttgtta ttttgatatt ctaaatcatt ttttattgga
       61 ttattccctt gttcgatata gacccaaatt gatctatcaa taattatcaa actgagtcat
      121 atagagacaa aaatttcaaa aatgacctgt taaatggtca tcgaatatca tttaaaatca
      181 atacgacgga gtcagacacg gagtcaaaac aagcccgctg agagtaaaaa atatatacag
      241 tcccataggt aaagtgccac aggactgtat atttttttat ttgagattac ctgaaatgat
      301 tccatttact ttttatctca ttttttccta ctagttagta tcaaatactc tcctattttc
      361 tcaataaact attttgggtt acatggtatc tgaaacagct aaaaccttgt cattacagca
      421 aaaaatcact ttatctccgt ttcatttcag atacatttca cttaagcgga ttccaaaaaa
      481 acaaaagaaa aaggaacccc tagttagcct atgcttttta ttggttcctc tcaaaacacg
      541 gtcatataac aacggggtgg ggtaaatgaa tgaaaaaggt ggaggaaaca ttattcaata
      601 gctgtcttaa atacttcgtt ataaaatgtg aacaaataaa agacatctat ccctattttc
      661 tacacttcca ctttactacc atctttgaat cgtacaattt tcttaactct tctcgtttac
      721 ctcaattcat tttcaagtgg aatcagctca tcatctaaca ttttacgcca tgctcgcttc
      781 atttcttgtg ggtatttaaa tggcttcgct tctggatggt tggctttata tccttcgtaa
      841 tccttactgt tgataattgc tttcatatta ggaattggat cttcacctgg catatattgc
      901 agattagaag catgtaattc tttctcagaa acaaaccact cattggaaaa gacatgaatc
      961 aaatcatcac tcgcttgtgt aaaatagcga cgtttaatga caaagatatc ttcattacta
     1021 tctaaagtcc ccacatcaaa cgcatttaaa atttgtcggt aaacttcttt gactccttct
     1081 gttttattca atttagccaa agctttttca atttcttctc gtgcatctga actattcgcc
     1141 gcataagttc ctagtaattg atcaatataa gtagaatcaa tatcatacaa cttcgtggta
     1201 ttttcactat aaaattcgat cgtggaaaaa tcttctccct ctttttctgg ttcttcctct
     1261 attaatgaac ccttcacggt ctcgtacacg ccaatttgtt cttccaaaag ttgaatttga
     1321 ttgttcaagg ttgaagattt caccatgtca tcattgtaat cgtcataagt cactaacgat
     1381 tcataagcac gattcaattc ttggaatgct ttgacgtact cgatacggat ttcaagtggc
     1441 gtatgttcat caatggattc catggatgaa acctctttta acttaccata tgcttgcttg
     1501 aatctttttt tggattcctg ataacttgga taaatcaaac ccgattcttc ttttgcttct
     1561 gaatacagtt ttgtcgcatc ctcgacattc ttctccattg tggctggctt tcggaaagta
     1621 acaattagtc cttttgtttt ttctggatac gtacggttcg tacgtgaaaa agcttggatc
     1681 aatcctgcat actctagatt acgatccaca aatagggttt gaatcgtagg tgcgtcaaaa
     1741 cctgtcaata gacgatccac tacgatcact aaatcaattt gtttcccaaa ttgtttgaat
     1801 tccgctcttt ttcgtgccaa acgattattg atgtctccgt tataacgctc aatgtctgct
     1861 aatgaccagg aagtatcata atactgatta tattcctcaa taattttttg catttcttct
     1921 tgttgggcac ttgaatctcg gctattttct tctaatgaat aggtaatagc gattcgtgga
     1981 aaatcaggat cctccatcgt tcgtccttca cgaatgagct gattgggaaa ctcttttgtt
     2041 aaccaatcag gctctttcgt catctctttg atggcccgat aatacttttt ggccatgtca
     2101 atggaactcg tggttaagat tgcagatttc gttggcctgc catttcgaaa atcaaatttt
     2161 gtataggcat tatctggacg gaacatttta tgaatgactt tttggatatg ttcatctgct
     2221 tcaaaaacag ctgggtccaa ataactttct tttttgacag gttccatttg atcaatcata
     2281 cgattgatct gttctgatga atactctgca tatgtttcta cttcttttaa tttccgatag
     2341 attttattct ctagtgaagt cggctccacg gtgttttcat gctctacttg aaatcctaag
     2401 acagagccat cttctagcgc attcttaatg gtataggtat gcaagacctc tccatactga
     2461 tcatgggttg tacgtgccaa ttgccctttg gcttgtttcc gattctcagg aaaaatcggt
     2521 gtacctgtaa agccaaacca agtggatttc ggaaacattt ttttgatttc ttccatattt
     2581 tcagcactta acgctcgatg acactcatct actacaaaca cgatatgttg gcccattaat
     2641 ttttggaagc gattggttcc ttttttctct tcttgtttct ttgcatattt caacgccgca
     2701 tctagttttt gacgggttgt aatgattaca acatttgcat tggcatctgc caacagggtt
     2761 tcacttaatt ctttggcact tcccgttcca acgatcaagc tattcgcttt ggcgttcccg
     2821 gaagaaatcc cagtattaaa ttccgaagca aattttgtaa actctgtggt ggtttgatta
     2881 tctaaatctt tgcggtccac taacatgatc gttcgatcga tccccgattt tcgtgctaaa
     2941 agcttagtag atacaaaact agtcaacgtc tttcctgaac cagtcgcatg ccacacatac
     3001 cctgattgat gtttatttgc ggcaataaac aaggcttgaa tcgcatgtac ttgataagga
     3061 tgtaacacca tgagggtttt attatcttga tcttcactga cgattgtata atccgcaatc
     3121 aaacgatgag catccgggat atttaacact tgtttacaaa actcatataa gttttctact
     3181 ttttcattgt ctttcgttcg ccaactaaat aaaaacttag ggtgcaagtc tttcggtaag
     3241 gcatttgcaa aataccgagt ggtttgttca ttggaaacca caaacaactg taaggtcgaa
     3301 aagatattgt tccgaaacat accttcttct gcatattttt tgatttggtt aaatgcctga
     3361 taaacaccat cttttgcagt tacttgtttc agctcgattt ggacaatcgg taatccattg
     3421 attaataagg tgacgtcaaa acgtcgatcc cgtgcttcta tgttacttcc acgttttgca
     3481 atttgatgga ccacttcata agtagagatt cctccaccaa tgtcttgatt ggaataaagg
     3541 ataagtgaga cagaacccaa ttgcgaatct tcccgttcaa tcgtgatccg agccatccca
     3601 ttttcacctt ttaaccattt cgcagcatca aatggggttt tcgttcgcag cagtaattcc
     3661 gttttgatcg tttcaaattc tttatcggtc aaaggaaaat cattcagttc tgcttgatta
     3721 tttgagataa ttttttggcg taaattgacc caaaggtctt cttctgattt tagatccgga
     3781 cgatacgtcc actgattatg cccttctcca agtacacgaa tcaactgttc ttcgacttct
     3841 gcttcctcac ggtgactaat tttcattctt cttccctcct agacaaacat cttttgtaag
     3901 aagctttttt tcgtttcttt tagtaaatct aacttacgtt gatgaagagc gatcgtatca
     3961 tctagctgtt tgaagaaagt accgattttt tgttgttcat cctttacagg aaccttaaca
     4021 ctgatattct caatatcaga actgttataa gaaggcaatg cccctacttg ggagtatctt
     4081 ggcaaattta tagtttcaaa caatatttta ccaaaataga tatcccaagt attattaaat
     4141 aaataagcca tggtattgtt atctattaag aaagggcttg tgaccaaacg ttttctatta
     4201 agcattattg ctgcgccaac tttagcaaat ataatcgctg gcacatcttt tattggcttc
     4261 cattttttac gctcaatttg ctcattagat acataatgat ttgcatttct catttcatac
     4321 tcattaccaa tgttattcat gtcggaaact ttaaaaaatg gagtaccttc agaaccacct
     4381 tgctcggcat caggaaatcc aattcccgat tgagttttac ctaattctcc caacttacgc
     4441 tcttcccaat cttccgtaaa accaggaaac cggatttctg gcacttttgc tccgttttca
     4501 gggaacattt tttgtaagaa tccttttttc gtttccttca gcaaatctaa cttacgttga
     4561 tgaagagcga tagtgtcgtc gagttttttg aagaatatgc cgattttttg ttgttctttt
     4621 aaatcattgg gaatatagaa tactgtatct gaaacttttt tccagtctga acgaggcatt
     4681 tttgttccag atgtatcatt tgcagccttt tgatatctat cagcttgaat aagagaataa
     4741 ataaagtccg agtcgctctt cttagctcta aaaacccaga aatccccaat cgctacgcct
     4801 gtaaaagttg ccttgagcca attttttaaa tatggacgta actttccata tagaatatca
     4861 tttggtaaaa attcaatccc ttttctatta tcaaatttct ggctcacatc tttattcagt
     4921 ctaccttctc ctgccacgat atcttcaaat tcaacttttg gaagtctact ggagtttgta
     4981 cttttattaa gtctaacaac taaatcaatt aacttacgct cttcccaatc ttccgtaaat
     5041 cctgggaaac ggatttctgg tcgtgtatca ttgctcatgt ttgaacacct ccagcgcacc
     5101 ttgtaaccac atttcgtttt caggtgttgt ttgcagggaa gagatagctt ctaaaagttc
     5161 tttttctagc acttgctgtt cttgtcgtat cttcttgatg tcatttccga catgaaccat
     5221 atcaattggt tcttcttctt cgaaagtatc tacatagcgt gggatattta agttatagtc
     5281 attttctttg atttcatcaa aggttgccaa atgagcgtat ttctcgacat cttttctttc
     5341 agcgtaggtt tcgaggattt tttgaatgtt ttcttccgat agtttatttt gatttttccc
     5401 tttaacaaat tcacgactag catcaatgaa taatacatct cgagtttgac gattcttctt
     5461 caacacaatc accgtcgttg gaatcgatgt cccaaagaac aagttcgcag gcataccgat
     5521 cacagcataa atactgccat cttctagtaa tttttgacgg atgacccctt ctgcagcgcc
     5581 acggaataaa actccgtgtg gtaagacgat ggccatggta cctgtttcct tcaaatgata
     5641 gaacccatga agaagaaacg caaaatctgc tttggatttt ggcgctaatt tcccataacg
     5701 attaaaccga gaatcatcta agaaagtcgt atctgctgac caattagccg aataaggtgg
     5761 gttcattact actgcatcaa atgtgtaagg ttcatctgtt ggccagtcct tattcaacgt
     5821 atccccattg cggagattca tttcttcggc gtcgacacca tgtaaaatca agttcatttt
     5881 tgctagattg tatgtcgttg tatttagttc ttgcccatga tatttaacgt tatctgggtg
     5941 tgttaagtag ttacgtacgt ttaacatcaa tgaacctgaa cccatcgttg gatcaaaaac
     6001 actaaagaat cgtcgttcct tttgatcgag cgttacgatt tgcgccatca tatcagaaac
     6061 catatgaggt gtgtaaaatt ctccggcttt cttccctgct tctgaagcaa attgactgat
     6121 cagaaactca tacgcatccc cgatcacgtc cccatcgtgt tctaaaacat ctacatcatt
     6181 caatttcttg atgacttcgg taatcgtcac atttcgttgt tgctcatctg ttcctagttt
     6241 ttttgattgg agatccacat catcaaataa cccattaaat tgattgtagg tagatgccaa
     6301 ttgcacaaag gctttgttta gatcgttcaa ttggaacgtt gcttgttttg cttgatccgc
     6361 taagacgttg aaaagatact ctggtgcaat tgcatatccc aaaatatcaa cgattgtggc
     6421 aatcagatct tcttttgatt cttcatctga aagtaattca cgatacaaca ttgtttgttt
     6481 gcttactgtg tcatattcct ctaaggattc atctgctaaa agaaccactt gttccaacaa
     6541 tcgatcggat aaatatttat agaaaatcag tcccaataaa taatttttat attctgacgc
     6601 atccatttta ctacggagat tatctgccgc actaaataat ttttgattta attctgcacc
     6661 caaaaagata cactcctatc tcttatttaa agttgctata taccctctta ttttagcata
     6721 ccccctttta taaaaagcaa aaaaaaaaga aagaaactta ttcttgctaa taaactacgc
     6781 gaaaggagtc taacgttgtt tgatataact tagactgatc gttaacttat tttttatatt
     6841 tctacatagt tatctttaga accatttcag atacatatga aacaattaca tatgattttt
     6901 tcataaaaaa acagtactgt ctagtagtat aaaatataca aatttcttaa aaattttttc
     6961 actaaaatca ctacctgaaa cagtctaaaa cacttatata tcaacgttta atgaaactat
     7021 ctccgtttca tttcagatac aaataaatat agtcgggctc caaatccgaa aaaaaatacc
     7081 cccagtagct tatcctaagc tactggagat gtataagtac ggtcatataa caacggggta
     7141 gggtaaataa atgaaaaaga tggaagaatc attattcaat aactttttta aatacttcgt
     7201 tgtaatcagt tacctgtccg ttcttaaata ccacagaata atctttttac ccattttcaa
     7261 gtaaccgaag tggaatgtct ttaccaactt gctctgtcac tgcattactg atacctgtta
     7321 gattagctaa taaatctgat aaagattgag catctgtaga aatctctgta tcagtattta
     7381 tcgtgattcc tttatcttca tttacaagct tccaagtagg tggtatagct gtgtatgttt
     7441 cgatgaatga atcaattctt tcatctatcg tcttcccagt atctacttct gtctcattac
     7501 tcttagatga atcgattctt tcatccatcg tctgctccgt aactgcttct gtctcattat
     7561 tctcaccgga aactctcttc acacccaaac ttatatattg tccatcttta gtgtgcatag
     7621 ttaaaattcc cacgctttta ctaataaatt cactataacc agttctttca atagagactt
     7681 caatgtattt cacactatca tactcaaaag aaaaatagac atgtaaaagc attttgttgt
     7741 ttacatctac ataataatcc tttgactcta cttgagacct tcctatacgg aatacagctt
     7801 tactgtctga tatctctata ccagcatcct tattattaat ctgccaatct ccaagtagct
     7861 cttctggcaa gctagtagtt tcttgtgaac actttaccac ttcactggta gaggtcgaaa
     7921 cattcacctc cgcactgtta gaagaatttt ccaaggaatc aggagcctta gcacaaccaa
     7981 caagtcttta tatattgtgc agccgatatt ttcttcattc agtacaccct tgaaatatgc
     8041 agtgttagat aaagcagttc gtcctgattt gctgaggcac ccatctcttc ttgaacgtac
     8101 tccttgattt tcaaggccgc ccgatactct ttcggaaaac gctgcttcac tgaagccagc
     8161 aaaaaatcat ccttctccgg caactcttcc ttcttaatca ttcgggcaat aaaaaactgt
     8221 aaatgcgtca caaatcttaa gtaaagcatc gaatctttat caaccttttt ttcgggaaac
     8281 agatatccta gcatagccaa gatatccttt agtgctctta ccgagagcat ggtttcttcc
     8341 atatttgctt gttcactttg ggcatttaca atgtgaaacg caatgtttcc agcctcctct
     8401 tctggcaact gaacattcat ttttttgttg atcaattgga tagcatagca gccgatctca
     8461 aattcttttg ggtaatactt ttgaatttca atcagcaggc gattttgaaa aacaatgcct
     8521 ttttgactgc gttcaatggc atgcttcaag tgatcaacca aggtaaaaaa tagactttta
     8581 ttcagcttaa agtcaaactt ggcgttggca taatcgataa ttcgcttgac gcaaataatt
     8641 agatcatccg gagcagcctc aaacagttta atgtaatcct tttttagagc atcagaaagt
     8701 gaatctaatt taaatacttt ttggattttg tcagccggaa ccaaatcgcc tttctttagt
     8761 tgaaatccaa tccctttccc catcaaaatt acttctttgc cttgcttatc taagcaaaga
     8821 attaaactgt tgtttaacgt tttaattact ctcattagat tcaccgttct ctctaagcta
     8881 agccatttct tgcgattact ttttgatacc aaaagaagct ttcttttttt attcgtttta
     8941 attctttaag attctcatcg gttcggtcaa catatataaa accataacgc ttattgaagc
     9001 cttgatgggt actaacaaca tcaatcgctg accaaggtaa ataaccaaaa acatctacac
     9061 catctgttaa agctgcttga atagccagaa tatgtttggc tagataatca attcgatcct
     9121 ggtcattaat tcgtctatct tcctcaacta tgtcgggtgc cccatagcca ttttcagtaa
     9181 ttaaaatagg taaatcatag cgatcgtaca atttcctcaa tgttaatcgt agtccagtag
     9241 ggtcaactac ccagccatat ggcgttttat ctacataagg attttcagct gcccggtaga
     9301 caccttcttc tcccaacatg atctgttgat cccccgctct agcggaaaca tctgatccgt
     9361 catttttgct ggcagcgatg gtggcagttg aataataatt aatcgcaata aaatcgggtt
     9421 taccggagga taaaattgcc tgatcctctg cctcaatttt aggatataat ccacgatcat
     9481 tcaaatagtt ttcaaacaaa taattgtatt ttccccgaac tgctacatct aagaaagacc
     9541 agcctctcaa tgtttcccaa ttatgggctg caattgcatc ttctggactg gaggtagctt
     9601 gatacatagc cgttaaattg attgctggac cgatttttcc tttaagattc aactgatgat
     9661 acaagtgaat tactctagct tgagctaata aaacatggtg attaatttga aagagttctt
     9721 tttttgaagg cagctctcca cttggtggca aaccaatcgc tccaggatga aggatcatcg
     9781 tgttttgctc attaattgtc agccaatagt tcaccttgtc accatactca gtaagcaata
     9841 cctgtacata gtccacaaaa taatcgatag ttgctcgatt taaccaaccg ccctgctcct
     9901 ctagaaaata aggtaaatcg aaatggtaca tggtgacaat cggttcgatt ccctgattca
     9961 ggcattcatc aattacttga tgataaaaag aaattccctt ttgattggtt ttattatttt
    10021 caatatccat aattcgcgtc caagcgatgg aaaagcgaaa agcttttaag cctaattctt
    10081 taaacagctg tatatcttct ttatagtgat ggtaaaaatc cgaggcaact tcaaaattag
    10141 ctaccccttc tgtatgctga tacttgtcaa taatagacgg ttgtttccca tcctcctgat
    10201 aggcaccctc tgcctggtag gcactaatgg aggcacccca taaaaacttc tctggaaatg
    10261 ctgaaagctg tttgtattgc acaaaaatcc tctccttaac taatttgcat caatggttcg
    10321 tgttccttta agcttccgct tgcttcacta attcctgcgt aattttcact gttggtcact
    10381 accacaatca ctgtatcatc gtagccagcg tttctaatgg cagcgaaatc ggcgttgatt
    10441 aataattgtc ctgctgacac ctgatctcct gcttttacaa atacttcaaa gggttcccct
    10501 tttaaattca cggtgtcgat ccccacatgg atcaataatt caataccttc tttagaacgt
    10561 agaccaatcg catgtttaga ttctaaaata gtaatgactt ctccctcaac aggtgaataa
    10621 atgctgtttt cttctggatg aatggctacg ccctttccca atagtccctt agaaaagaca
    10681 gcatcgttca cttcagaaat aggacacacg gttcctttta ccggattttc aacaatgact
    10741 gcttcggcta ttgccgtttg ctctgattca tcagaagtat cgtcaaatcc aattagataa
    10801 gcaatgataa aggttgcaac aaaggcgatt acacacgtaa taattgcgtg aacaatattc
    10861 attggatttt ctccaataaa ggctggtaag gctgctaagc caggggaaac aaaggcatat
    10921 ctgactaaac cgctcaagcc agcatacaaa ccagcaattg caccaccaat catggaagca
    10981 actaatggtt ttttcagctt caaggttact ccatagagtg aaggctcagt aattcccatt
    11041 aaagcagtga tcccagtgga cacagctagc tgcttcaact ctttgttttt agttttcgcg
    11101 gctacagcaa aggtagcacc tgattgagca atattagaag ccagcattcc tggcccattg
    11161 atcatttcag aacccgtact gctcaattgc atagtagcga ttggcgtcat tgcccaagct
    11221 gtacccgtaa ccaccaaaaa tggctgcaaa ccacccatca aaaatggaat taaccaagct
    11281 gctttgccgt taatccattc agcccccatt gctataagat cgttcagcaa cgttccaaac
    11341 ggtccaatga caaccaacgc taaaggcccc ataattaaaa ataccagcat cggcttagta
    11401 aagaatttaa taaacgaagg cgaaattcga tccgcaaagg gctctacata ggacatgatc
    11461 caaacagtaa agataatcgg taaaactgat cccgcataat ctgcagctaa taccggaatt
    11521 cctagaaaag aaacggcatc gccagtagaa aaaagctgtg aaatatttgg atgaagcaaa
    11581 atgccagcaa cagtaatcgc caaaattggg ttgacatcaa actttatggc tgccccatag
    11641 gctaatagta ctggcataaa ataaaaggct gcatcagcaa tagtattcaa aatgatgtaa
    11701 gtttgactac tgtctgatag tacaccagtc aaagtcaaga cggccaaaat ggcttttacc
    11761 atcccggcac ctgttatagc tggaatcatc ggtgtaaagg tggtagaaat gacactaatc
    11821 aatcttgaaa taatactttc ttgattgtct gaagaggggc cctttttaat tgaagttccc
    11881 acttcctttt gaatttcctt gaaggctgtt tggacttcat tgccaatcac cacttggtat
    11941 tgtccaccgc tatccaccac actaattaca ccaagtgttt tttctaaggc tttcgtatcg
    12001 gctttccttt tatctttgag ttcaaaacgc agtctggtgg cacaatgggt taagctgacc
    12061 acattttctt ttcccccaac ctgttgcaat acatttcttc ctacttctct gtaatccatt
    12121 attctctctc cttaaagcag taaaaaagac caagtcaaac cacgccccaa tcaacattga
    12181 agtgttagtt tgtcttggtc ttgcctgcat aatcagtaac atgccaataa cgttattttg
    12241 ctgtaatcca ataatagcac attatgatag cgcaatcaac agggaattta attttttatc
    12301 ttttcgtttg accgtatagg gtaatataac agttagatca catcaactag ggtatatctg
    12361 cgtatactta ctgctaaaat aagtatatct aatattaact ataccagtaa aaccattcct
    12421 acataaccag cgggaaattc ccaaaatcgt gaaagcatca aaaatgcata taacggatca
    12481 cacaacttca acaattctgc tcttgggaaa aattgatgaa tttggtgagt aaaggatgga
    12541 ataaacagca ttccaacaat ataggcaaca atagtgatac ttgttttttt ggtaaaaatc
    12601 agtaagcaat agccaatttc aatcattccg agatataaag gtatcaatag aatgatttgg
    12661 gctagtattc ttaaaaatta actagcaaat gtttaaactg atttatttct aatctacatg
    12721 tagtaagtat ttcaattcca ccaattatca ataaaaatac caaacatatc cataataatt
    12781 ttttcttttt catttctttt atattgttat aacagtgaat aacataaaaa ttcccgacaa
    12841 tcattgtaat atttttttgt cacctgtatt agtatttaat ttataggaaa ctataaaaac
    12901 tccctagtga atttacacct ttgggagttc ctgtttggac ggtatgcata atacatttaa
    12961 atcaatttaa cattaacacc tcagcatatg gtatctccac atatatacgg tctttctttg
    13021 gatagtactc aatatttatg ttgatagcac ttccattgtc taatatagct ttagcccggt
    13081 atgtgttatc tccattttca tgagtaatcg tcacatcatc acatgatcga tttaagttgt
    13141 actgttcttg taaaatctgt gttaccaatg gttttgatga cttctctaat ccagatgtac
    13201 ctgtcatttt tgaaaaaata atacctataa tagccaagac aacaatacct ataatgacaa
    13261 acttcttgtt catatttact ttattcaact ttttatctcc tatctccaaa ataatttgtt
    13321 cttatccctc ttatcagtac attgacatcc ctaaaagtac accaaagatt gctccagcta
    13381 cccctgctat catcaaaatc gtacccattt tctcatcttt ttctcgatat aagtagccca
    13441 taataacagc aacagcacca aatacaacag gaataaatgc taacgatact accatagaaa
    13501 tccaaccaat aatcaaatac agcttccctg tctcttgtcc atgtacgatt tgttccttca
    13561 tttcattaat tgtctgctca gtacttttct ttactgtgtc tgttccggtt gtctcatctg
    13621 tttcttgtag tgctccacaa aactggcaat acttactttc atctgttatt tctttgccac
    13681 attttctaca atacatgtct tctcctccta aaatcattca attgcttatt agttaactgt
    13741 ttactttatt aaatacatca gaaaacagct cattcattgg tacacttaat atctcagaaa
    13801 gcatcttcat ttgatagggt ttaggaatcg ttctccctgt ttgccacgaa cttacacatt
    13861 gctgtgtaac attcattttt tttgctaact ccgtttgaga aagtcctttt tcttttctgt
    13921 atcggctgag attacttact tttttcatga ttctcccctc ctgttttatt atatacaaca
    13981 atatgatgta tttcaactca taaaatataa aaaacttcaa ataccattac aaatacaaca
    14041 acttgttgta ctatactgag atgggggtgt attaatgaaa cagaaacaat atgcaaaggt
    14101 cattagagaa aagcgaaaag ctctcgggtg gacacaaaaa gaattagctg aaaaaatctt
    14161 ctctacacaa caggcagtag caagatggga aaattctgta acagagccta acctcgatag
    14221 cttaacagct ctttctagag ccctaggaac ccctgtaagc cattttctag acaatgttgt
    14281 cgtagattat gaggaagagt ttttggctct ctatcgttct cttagcactg aagatgcagt
    14341 tcgaacaatt gactatatga agctattaaa aaggcaagaa aatgaacgca atcagctatt
    14401 gaaagattga aaaataaata acccaccgaa ctttaacggt gggtgtattg agatgaaata
    14461 agattaagag ctatggataa cctgtctagt ttaatacaat tgggtctttt ctcttcctta
    14521 ctctccgtcc atcatctctt gttacacttg taaattgata cagaggaact atcccagctc
    14581 ctgataatat caattctttt attgtttcat ctatccctgc ccctttcgct aaatatatta
    14641 aaaaactact acttgctttc acattgtata gtttcttaat atccttagaa gctcgttcaa
    14701 gagtgaatcc ttgtttaaca aagtagcctc tgttttttat taaagcattt ttcacgctgt
    14761 tatccccaac attacgtagg cgttcacaaa cagtgttaat aagttctttc tctgtcatta
    14821 gatatgtccc ttgccacgat aagccctgta aaacttcatg tgcttcatct aaaatcaatt
    14881 gtttttcctt gctgagttct gatcgggaat tttgaggaaa tactctttta gcctcttttt
    14941 cgttaaaaca tatcttaacc cattcataag aaaaatattg atatgtaaaa tgaatttttt
    15001 ccagaagtgg aaggatatct tcattcaatt tttgaatcac agaagttgca ttaaaaacgt
    15061 ctaatcgata gacattgctt ctcttatgat taaagtttcc taacgctttc tcttccttta
    15121 acctatttag gtattcgagc atcttcaaag gaacatcttc atcatctaat ttttttatca
    15181 atcctataaa agtcataagt gatagtaact tattacattt atctatgtca taagttttac
    15241 catagtaaga actcattttt ttagctaatt gttcccccga caaaatacaa gtcacacttt
    15301 ccttactatc atcaaaaaaa ttaacatcag aaagcaagac atcatagaca cgtcgcattt
    15361 cctctagata attccttatt gtcctgaaaa gtttgtcttt cattgattca agagtgtcaa
    15421 aaagtacttt tgattcctct ttcaattttc gaaaatgtgt aggatacaag tctaggttct
    15481 ctaatagcca attaaatgtt tctacccgac tgtctttctt taaaattagc atcacaactc
    15541 caataaaatt ttcactgata tcacaattac tggaatgaca ataaaaaatg gaatgcccag
    15601 ttttactggt aaaaatacta gcagagggat tatgatcgtt gtgaaacaga catcttaagt
    15661 ttttacttga cttaagtaat ttattcatat caatggttaa caatctttca taaatctcat
    15721 taaaagacat gtcggaggaa tcaaacaggc agtctttaaa ccactgtttc atttcttctt
    15781 tgtcgtcatt ttttatcaac ttaacaaaat cggtttcatt gactagttca tactctctcc
    15841 ctgaatcact ttgacaattt ttagaacatg tcatggtaaa atcaatcgga agctcagtaa
    15901 tatctaacat gttgtcttca ttaattatta tcactgcatc tgacttatat cctccaaaaa
    15961 acattctatt cgaagcattt acattgtcat cacacccggg gatgttcttt ttcaagtatt
    16021 cgtaaacagc aaccatttta ccatagtcag taattgcttc ttttaataga aagacaatct
    16081 tgtatttttc tagattttca gagtaccgaa tacttttttg tatcgcaaac gcagaattat
    16141 gaactaaatt gatagataaa accttttcta tgcttaagta tctactatca tctttactca
    16201 aatctactac cttccccttc tcatctttaa cagtgttatc gacatccaga ataattactc
    16261 tggtttccac aaaactctcc ttgtcattgc ctttattctt tgtcttgctt ggaataaatg
    16321 attgtgatga atttagtatc tctacaaact catctcttgt aacttctcta tagttatcta
    16381 aacacctatc tcttacatca gttttttctt tttttgtaac tggcttctct aataattttt
    16441 cttgatctac ataaattgga atattaatca ttgatgtttc catttgtgtt tcctccttat
    16501 aaaattctgt taaagactaa aacatacaga taataaaaac aaactcttta ttactatatg
    16561 ttttagtctt atttgtttac tcaaccttta taccgttcca tgaaactatc aatatcagat
    16621 tttttaaatc taatgaccgt atcaattttt ataacaggta acccgtattg agaaacatat
    16681 tttctaatcg ttcctactga tacacctaga tattcacttg cctgctgata gtccatatac
    16741 tctagagtga ctccagaaat aatgtcttct agcataggct ttagcactgt aattatctct
    16801 ttcacttgtt gttcaacttg tatagaaaca atgctatcaa tgatttgtag agacatattt
    16861 ccccctcctt tcgtgattgc ttgaacaaat tttctaagaa ttatcttcgt attttttgct
    16921 caaaataaaa gtattttgtt attaaccgat taatcaaaca aaaaatgatt accagttcat
    16981 cgacatacac ataatacctc gataaaaaag gggtgtcaat agttttgtaa aatttattta
    17041 caacacccct tttttgaggt atactaattt taggaggtgt ggtcaatatg ataaaacgtc
    17101 caccaataaa ttatttagaa cgaaaaaaga tactcggaac aaaaataaaa gctataagaa
    17161 agagtaaaaa attaacacaa ccagcatttg gtcttatgat taacaacggt caattaattg
    17221 acaaaaagac tatctacgaa tgggagaagg gaacctattt accaatacca gagcgattat
    17281 cccgaattgc tgatttggga aatatgagta tagaggagtt agtttgtggc aatgtagaag
    17341 aatatatttt gggaattatt ctttatagag atagcattgt actagatggt ataacatttc
    17401 cagataaaaa cctgttccag catcttcgtc aacagttccc accagtacac tctaatttag
    17461 atacatggct ggatagatat tccaaattag aaccagagat gcaagaattt attgctaaca
    17521 agacttgtaa taaagttaaa aatgaaaaaa ttagtctttt caatatctta aaaatagaag
    17581 aattattcat caatgctatt gtagaagaat ttgacaataa cattcttttc ctcactagca
    17641 gtatagaaga actactagaa agaatggtag atgaatggct acctattcaa ttgaaagaca
    17701 tgtcttatcc cgaagaagct gtgagagaaa ttactgataa tataaacaag ctagaacaaa
    17761 cgatttcatc aattgggaaa aaatacacta agaaaaaaat gaagggaggt gataccatct
    17821 agtggtaagt actccttgta aactaagagg agatgatttc atggcaaaaa tatcaaagta
    17881 caaaaagaaa aacggtcaag tggcgtggat gtataaagga cacattgcca ccgatccacg
    17941 aactggtgaa aaaataaata ctacgagaag aggttttagt agcaaacaag aagctcaaaa
    18001 agattatgac gagtacagac atcaaatatt atacggtgtt aaaaagaaag cacctgatat
    18061 gacatttgaa gacctgtata atgaatggat aactcatcag cgtaccagtg ttaaagcttc
    18121 aacaatcgca atctcagtaa gatacgcaaa taatcaaata cttccagcat ttggtaagct
    18181 taaactatcg aatatctctg tcccatattg tcaaaaagtg gtagatgaat ggcacagtaa
    18241 atatgaaagt tacgattata tgagaaaaca gactgcacaa attttacgct acggggttgc
    18301 aatgcaatat attgacaata atcctatgga aaaaacactt cttccacgaa aaaaagagta
    18361 tgagaaaaat cgtaaatttt actcaaaaga ggagcttaat aatttactgg atgcttttaa
    18421 agattttggt aacatgaaac aatacgcctt cttcagatta ttggcataca caggtatgag
    18481 aaagagtgaa gtactcgctt tacagtggaa agacatagac acgtttaata aagaactaca
    18541 tgtcaataaa actcttgctg tagatgaatt tggtaaagtt ataattcaat ctccgaaaac
    18601 aagagcttct agacgagtta tctctttaga tactgagact ctttctatcc tcaacaactg
    18661 gaaattacag caaaaagaag agtatttaaa acttggatac aatactagct ctaaagagca
    18721 acatgttttt acaacagtta aaaacactct atacattcca aacactgtta atgattggtt
    18781 aagatatatt ttgaaaaaat ataacttgcc tcgtattaca cctcatgggt ttagacatac
    18841 acatgctagt ttattattag aagcaggaga atctgtcaag gtagttcaac aacgattagg
    18901 gcatgaaaat agtaaagtga cattagacat ctatgcacat attacaaata atgcacctaa
    18961 gaaaacaggt caagactttg ctgatatgat ggctcatcag taaaagagct ggtcaaaatc
    19021 tggtcatttc tagtatacag gcaaaacaaa aaagctagaa cccttatata tcaagggttc
    19081 tagctcctat aacaggttta cccaacactg ccttccatct cataactgat caatcgattg
    19141 agttcgactg catattccat tggaagttct ttggtgaatg gttcaacaaa gcccatgacg
    19201 atcatttcag ttgcttcaga ttcggtcagg cctcggctca tcagatagta gagttgttct
    19261 tctgagattt ttgagacttt ggcttcgtgt tctaaagaga cttggctgtt gtggatctca
    19321 ttgaatggga tcgtatcgga tttcgaccat tcgtccatga tgatcgtgtc acattcgata
    19381 tgagagatcg aacccgcact gtttttggca aaggttactt gtcctcgata gtttacttct
    19441 ccgccgtctt ttgaaattga tttagagacg atagagctag atgtatttgg tgcattgtgg
    19501 atcatttttg cacccgtatc ttggatttgg tttgctcctg caaaggcgat ggagagcatg
    19561 gtgcctcgtg caccttttcc atctaagtaa acacttgggt atttcatcgt tgtttttgca
    19621 cctaggtttc cgtcgatcca ttcaacggtt gcgccttcgt atgcttttgc tcgtttggtt
    19681 actaagttgt acacattgtc tgaccagttt tggatcgttg tgtaacggca atacgcatct
    19741 tttcttgtaa agatttctac gatggcggca tgcaggctgt tacttgaata agtaggtgcc
    19801 gtacaacctt cgacatagtg gatgcttgct ccttcgtcaa caatgatcag cgtacgttcg
    19861 aattgccccg tgttttccgc attgatccgg aagtacgttt gtaatggaac atcgactttg
    19921 acgccttttg gcacgtaaat aaaggtccca cctgaccata cggctgaatt cagcgctgct
    19981 aatttgttgt cggttggcgg tacaagtttt gaaaagtact ctttgaataa ttcagggtat
    20041 tctttcaaag cagagtcagt gtcagtaaag acgattccta acttttcgaa ttcatctttc
    20101 atattatggt acacgacttc tgattcatat tgtgcggaag caccggctag ataagctcgt
    20161 tcagcttctg gtataccaat cttttcaaat gtatctttga ttttttctgg tacgtcttcc
    20221 cagtcacgag cgggtttgtc acttggtttt tggtaatatt tgatggcatc gaaatcgatg
    20281 tctgaaaggt cggggcccca tgtttgcatt ggcattttgt taaatgtttc tagagatttc
    20341 agacggaatt caagcatcca ttctggttcg cctttgattc ttgacatctc acggatcact
    20401 tcttctgtca agccttctcc tgtactgaaa accggttcga catcatcgtg aaacccgaat
    20461 ttatattctt ctaattctgg tacgcccatg ttctcactcc tcttccttgt ggtaatgatg
    20521 gaaagctgcg gtaccatcgt tttccattgc ttttcctagt gctttccagg caagtgtcgc
    20581 acatttgatc cgagcgggaa atttagctac accaccaagc atagcagcat cgcctaattg
    20641 gtcgggatct ttgacttctt ttccttggac aagctgtaga aagtctacag ttaaatgttc
    20701 tgcttcttct attgattttc cgatgacggc gtctgtcatc atcgaggcac ttgccgtact
    20761 gatggagcag ccactgccgt caaaggcgat atttttgatg attccgtctt ttacatcgac
    20821 ttgcaattcg atcacatctc cacatgtggg gttgttcaat tcgatcttgc ttgtcgagtc
    20881 agtcaagctt ccacgatgat gcggatggga agaatgatcc aagatcactt gacggtataa
    20941 attgtctaat ttagatagtc ccatgttgga aaaactcctt tgttgccatg attgcttcaa
    21001 ctaaacggtc ggcgtcttgc gttgtgttgt agaaataaaa gctcgcgcga gcagtcgctg
    21061 atacttcaag gtacttcaat agcggttgcg cacaatgatg acctgctctg acggctaccc
    21121 cttccatgtc taaagctgtc gcgacatcat ggggatgtaa cccatccaaa ttgaatgcaa
    21181 cgactcctgt ccgaaaattc gggtcttgtg gaccataaac agtcaatcca tcaattgctt
    21241 gtaattttgg caacacataa gcggctagtt cttcttcata tcgatggata ttgtccatac
    21301 cgagtttttg caaataatcg attgcatggc ctaatgcgat agcgccggcg atatttggtg
    21361 tccctgcttc gaatttccaa ggtagttctt tccatgtact gtcgtataat tcgacaaaat
    21421 cgatcatttc gccaccaaat tcaacaggtt ccatttcttc taaccatttt cgtttaccat
    21481 ataaaacacc aatacctgtt ggtccgcaca ttttgtgtcc gctgaaagca aagaaatcac
    21541 agtctagtgc ttggacatca atggccatgt gcggggcaga ttgagcacca tctactacga
    21601 tgaccccgcc gttttcatgg acaagttcag ctaattcttg aacaggattg aagacaccta
    21661 agacattgga tacatgagca atggagacga ttttggtttt gtccgtaatc tttttcgctg
    21721 catcctccat atccaggaag ccttcttctg ttagttcgat atatttcaag acagcacctg
    21781 ttcgttttgc caactgctgc cacgggataa cattagaatg atgttccatg taagagagca
    21841 ctatctcgtc tcctgctttg atatagcggt cgccgaaggt tctggctacc caattcaagc
    21901 ttgtcgtcgt accacgagta aacagcactt ctgctgtttc ttttgcatgg atgaacgccc
    21961 gtactttttc tcttgcttgt tcataatcat gggtcgcgcg ttctgctaac gtatggacac
    22021 cgcgatgaac attggcgtta tcgtgttcgt aatagtacgc taatgcctgg agtacttgtt
    22081 ttggcttttg ggtcgtcgct gcattatcca gataaactaa aggttcatca ttgacgtctt
    22141 gaaataagat aggaaaatca ttgcgtagct ttttagcatc gatcatccat tcaacttccc
    22201 ttcgattact tcgactaatt ctttccgaac ttgttctaca gggattgctg ttaagacaga
    22261 accgaggaat cctcgaatca ctaagcgctc tgcttcttct ttatgcaatc ctctactcat
    22321 cagatagtac atttcttctg gatcaactcg tcctacactg gcagcatgtc cagcagttac
    22381 ttcgttttca tcgatcaaaa gaataggatt cgcatcacca cgtgctttat cagaaagcat
    22441 caagacacga ctttcttgtt gggcatctgc accttttgcc cctttcaaga tgtggccgat
    22501 accattaaag gtcaatgttc ctttttctcg gatcactccg tgttgcaaaa tatgcccgat
    22561 cgtatgaggg gctttattgg ttactcgtgt atcgattcct tggatttgtc tgcccgagct
    22621 gattgccacg attttgactt ctgcgtgtgc accttctcct gctaaatctg agtcaaagtc
    22681 agcgatcaca tgaccgtcat tcatgacacc aattgcccag tcgacagaag catctcgtaa
    22741 aatgtgccca cggcgattca tataagaagt aatattttct cctaattgat ctacggctga
    22801 atatttgatt tttgaaccag ctttagcgat gacttccacg atgatattac ctgatgattt
    22861 tgctacttgt tctttggttg tctggaaacg ttccagataa gaaaattcac tatgttcatc
    22921 tgccacgatc aacacatgct tgaagaagtg ctcatcggat gcgccatctt gaatgaataa
    22981 ggactcgagt ggttcatcga tcacgacatt tttaggcaca tacaggaaaa taccgctgtt
    23041 cataaaagct acatggagtg cagtcaattg gtcttcgttc atgtttactg cttttttcat
    23101 ataatattct tggactagtt ctggatattc gatcattgca gtgaacaagt cggtaaaaat
    23161 gactcctttt tctgctaatt ccacaggcag ttgttcaaaa atcgtccatg aaccttgttg
    23221 gacaagcaca ggattgtctt tcattgcatc aaatgccgga atcgtgcctg tttcactgat
    23281 ggtttgtgtt tctttcacat caaataacga ccagcgatgg aatttcaccc gatcgatatg
    23341 cggcaatggc agttcatctg ctttcgctag tgcgtcttga cggaaggttc tcatccaatc
    23401 aggttcgcct ttacttaaag aaaaatcatt taccgcgtca agataatcga gccagttttt
    23461 ttctttcatt cttgctcccc cttacgcttc ttcttcttta tagtcgattc ctaattcttt
    23521 actgatacca gcgtagcctt ctgcttccag acgtttagct aggtcagcat tccctgtcat
    23581 gactactcgg ccttccatca tgatgtgaac gacatcaggt gtgatatagt tcaataatcg
    23641 ttgatagtgg gtgatgatca atgcaccgaa attgtctccg cgcatttcat ttactccttt
    23701 agcaacgact ttcaaagcat cgatatccag acctgaatcg atttcgtcta aaattgcaaa
    23761 tgtaggttct aacatcaaca attgcaggat ctcattccgt ttcttttctc cgccagaaaa
    23821 accttcgttc agataacgtt cagccatttc ttctggcata tttagtaaag ccattttttc
    23881 atctaacttt ttcaaaaatg ccataacaga aattttgtcg ttttcatcac gtttcgcatt
    23941 gatcgctgca cgcatgaatt ccgcattcgt gatacctggt atctcacttg gatattgcat
    24001 agctaaaaat aagccaagtc gcgcacgttc atctacttcc aagtctaaaa catttttccc
    24061 gtcaaacaaa atttcgcctt ctgttacttc atagtttgga ttgcccatga tagcggcaga
    24121 taaggtagat tttcctgtgc cgttaggtcc catgatggca tggatttctc cggttttcat
    24181 tgttaggttt acgcctttaa ggatttcttt ttcctcgatc gacacatgta gattttttat
    24241 ttctaagaca gacatgcatt atacccccaa attctctttc gttctcactc attttagact
    24301 aattgagaat gaagcgcaat gaccttcctt actaaaatca aatatctttt atttattatt
    24361 aaacaatttt gatttctaaa ttagaatagg tataaaaaag atgagaaata agccattaca
    24421 gacttatttc tcatcttttt tgttccatac cttattttgc atcagccttt actggtttaa
    24481 cagagccgcc ccatttgtct gtgatccatt cttggacttc tttgcggtga agcacttcaa
    24541 tcagtttttt gattttgtca ctgtcttcat cacctttacg agtcgcaata atattcacgt
    24601 aaggagagtt gtccttttca agcaatacgg catcttctaa tggattcaac cctgcgccgt
    24661 aagcgaagtt tgcgttgatt gcaactaggt caccttcatc attttggtac gtagaagcta
    24721 aaagtgatgg gtcgatcgta tggttgaact tcaagttctt agggttttct tcaatatcat
    24781 cgaatgttgc tgtttccaga tctactccat ctttgacttt gactaagcca gcatcttgta
    24841 gaattgtgat gatgcgtccc cagtctgatt cagaatttga agtaatgatt gttgcaccat
    24901 cttttaattc ggaaacatct ttgacttttt tagagtacaa gcccattggt tcgatgtgga
    24961 cagcaccggc attgacaaag tcatatcctt tttctttgat ttctttgttc aaataaggaa
    25021 tatgctggaa gtaattggca tcaatgtcgc cttcttccaa agattggttc ggtaatacat
    25081 agtcatcgaa tttgacgatt tcaagatcga ttccttcatc tttcaataat ggtttcacat
    25141 gttccaaaat ctctgcgtgc ggtgtcggtg aagcgccgac tttcaatgtc gtgtcgtctg
    25201 ctttatttgc tgaatctttg ctgtcgctgt tgttaccgca tgctgctaat cctacagtca
    25261 gcaaaagcgc tgctgcaaat ccaaaaattt tctttttcat atcctacatc cccctatatc
    25321 ctattatctt ttgtctgttt tcttagttaa gaaatcaccg attccttgaa cgataaatac
    25381 gattagtaaa ataattaaag tcgcaacgaa tgtcactgtt agattaccac gctggtagcc
    25441 ttcctgccaa gcaagacctc caagtccacc tgcgccgatc gcgccggcca tcgcagtaaa
    25501 tccgatcatc gagatcgttg taactgttaa tccagaaatc aatgcgggaa tactttcagg
    25561 tatcaagacc ttccagatga tttcccagta acttgctccc attgcatctg ctgcttccag
    25621 tacaccaggg ctgacttcac gaaaagcaat ctctactaga cgggcataaa atggtgctgc
    25681 ggataaaaca agagctggaa tggctgcttg cgcacctagc atagtgccta cgattgcttt
    25741 agtaaatgga atgatcaaaa ccatcaggat cataaatggc gttgaccgga aaatattact
    25801 gatgatcgat acgattccat aaatgattcg agcatacggt ttgtttttgc gtccaataga
    25861 gtaaaggatc aatcccaaaa ctaatccgat gatcgtcact aagaccattg agattaatgt
    25921 catatatagt gtatcaagga tagcctgatt catcgtttcc gggttgattt gtttaaaatc
    25981 tagatatgtt tctgaaaagc ttttatccac gatggatcac ctcaattcct actccttgtt
    26041 ctttgatgaa cacttctgct tgatctaatt ctttggtttc accagtcatc aagacagtca
    26101 atgaaccaaa ggaaccttct ttcacttgtt tgatctttcc atacacaacg ttgacatcga
    26161 tgttatagcg tcgaatcgct tgggaaatga ctggctcgtt ggcatttctt tcgctaaagg
    26221 tcaaagttgc caaacggccg cttggatttt ctttgatgag ttcagctaat agttctgtcg
    26281 aatcagcttc tggttcgatg tcttgttgta caaagcgttt ggttacttct tttttcggtt
    26341 gtctgaagac agtcaatacg tctccttctt ctacgacttt ccccaactcc atcacagcga
    26401 ctttgttgca gattttgcgg atcacattca tttcatgtgt gatcaagacg atggtcaaat
    26461 tcaatttctt attgatcgct aacaataagt ctaatacttc ttctgttgtt tgcgggtcca
    26521 atgcgctagt agcctcatca caaagcagga tctcaggatc atttgccaat gctcgtgcga
    26581 taccgacgcg ttgtttctgt ccgccggaaa gttgtgaagg atatgcttgt ccgcgtcctt
    26641 ctaacccgac taaacgcaac agttcttctg cccgttcttt acgtttgctc tttggtacgc
    26701 cagctaattc cagcggcagc tggatatttt caagtacagt tcttgaccat aacaggttga
    26761 aatgctggaa gatcataccg attttctttc ggaatgtacg taattctttg ttttttaatt
    26821 ttgtaatatc ttgatcgttt acgatcactt cgccgctcgt gggaagctca agtccattca
    26881 ggagacggac taatgtactt tttcctgcgc cagaatagcc gacgattcca taaatatccc
    26941 ctttttcaac gtttagtgaa acgtcattga cggcttggat gtttccgttt ttcccattga
    27001 atgtcttttt gacattcgtt agttggatta atgacatgcc attcacatct ttctcttata
    27061 atttttcaaa caaaaaagcc ttcgccctct tgccttaagc aaaaggacga aagcttgaac
    27121 ttccgtgtta ccaccttgat ttaccgttac ttcgcaataa cagcctcttc cagtacttgg
    27181 acttagcttc tttccgtata ctgtcgcgct gtaacgggca ctcccgtagt aagtttgctt
    27241 acgctcgctt actctgctct aagaccatct tccatctcat tttcgttacc cattttcagc
    27301 aaacagggct ctcttcaaac gtccgtgaca tgtactcttc ttttcaaagc tttctcgttt
    27361 catataggat tgcttatgat tatagcaact ggaagaatca gtgtcaactt ttttcaagtg
    27421 attttctgat tttataattc gtcaaattct tttggatcaa catctttgaa acttaagatc
    27481 cacgcattca tttcatctga atcattcaag atattgatat cttcaccgat tttatcatta
    27541 acagaagaaa ctgtacccgt tagcgggctg gcaaattcat tgaccgcttt ttccgcttca
    27601 acttcgatca gaggttcatc tttttttagc gattgtcctg cttttggcag attggcaaac
    27661 gtgatgtctc caagttcttc ttgcgcttca tttgtcaaac caacacagta ttcttttcca
    27721 ttaaataaga tccaaaggtt gtcctttttc ttcaaatgct tgcctgccat tttatatcag
    27781 ttctccttca taaatttttt cattgaatcc aattgcaaca agctggtcct ctcgtaagat
    27841 cagtggtcgc ttgatcagca taccatctgt cgataagact tggctcgctt ttgaagccgt
    27901 aaactcgcct acttgatctt tcagtccaag ttcccgatat ttcatcccgc ttgtgttgaa
    27961 caggcgacgg attggtactt gactcgtttc tagccaagtc tcaatcgttg aggccttcgg
    28021 tacttctttg atcatatcaa cggtttgata actaacgcca tgctggtcta accacgcttt
    28081 ggcttttttg catgttgaac atttcgggta ccagtaaaag gtgtacaatt ttcttacctc
    28141 cttgaagatc ttgcaggtgc ttcattttgg tatctcatcg ataatatcaa cccgataccg
    28201 atcatgttcc ctaaaatcga ggatcctccc tgactaatga aaggcaatgg gatccctgtt
    28261 aaaggaagta atccgatatt tgctccaata ttctcaaaga catggaacag cagcatcatg
    28321 atcaaaccgc ttgcgatata ggcgtaaaat tcattattcg tatcgaaaca cacccgaatc
    28381 atacgataga ttaaaataaa atacaagaaa atgacgaatg caccaccgat aaagccgaag
    28441 ttttcaccga taacggtaaa aatcatatcg gactcacgaa caggcacata gatattgctt
    28501 acgttgaatc ctttaccaaa caagccaccg gtaccaattg ccaaaagtcc ttgggccggc
    28561 tgataactca ttcctgacgt atcatggaaa gggtccagcc atgaatcaat acgtgcaaac
    28621 tgataagatt tgaatccaac tttataaagc aagtctcttc cgccttcagt cgtaaccaag
    28681 aagatcgttc ctgctccaac gactactgcc aagattacta caggtgcgat gatgcgccaa
    28741 gagattccag acatcaggaa aatcccgcca aagatcgcaa gaaagacaag catcgttcca
    28801 aagtcatctt gggccatgat caatgctaaa accggtattg tcacgatcaa gagcttccca
    28861 atcagccaaa agtccgattt catcgtccgt tcttttactt gcgtattatg tttagttacg
    28921 atcagtgcca tcatcaagat gtacgcaatc ttcatcaact ctgctggctg gaaagtaaac
    28981 gcaccgaatc gaaaccagtt tttagaccct gttgaagcag ctaaattctg atcatagaat
    29041 ttgagcaaga gtcccattac cactaacccc aaaccataaa ggtaaggagt cagtttccag
    29101 atccatttgg aattcaaatg catgatgaca acgattgctg ccgctccaac taaataccag
    29161 acaacttgca tccctacgcc tttcaagact gctggattgt tcggatcatg gctaagtgct
    29221 acatataatg agagcagacc aatcaaacat aacagaaaga ctggtaaaat cacgccataa
    29281 tcaatacgat tatcgttcga agcttttaat tttttttcca tgtttcatcc cttttcttaa
    29341 aaaccatact gctgattatt atagcggtcc agttagataa tggaaagtct ttccatagac
    29401 atttgggaaa aattaacttt ccgtttgtgc agttgcttga gcaaatgcag tacaaaagcc
    29461 tactgcttat ctttagtgta tcatagaact ttttttgata catgcaatta tctaccagct
    29521 gagattctcc tcgctgtgag aagtttctgt gctttcattg tatcgaagga tctcttcggc
    29581 aatctctagc agctgatcag aaaccagatt ggttcgttgg atgaaataaa aatatcgatg
    29641 gaaattcaac ggagtcattg gcacattttc tggaacagag cgcttagaca aaatcgagcg
    29701 accgattcct tccttcagca acgcaacaat tacatcgtta cttttcacga tcatcttctt
    29761 atcttggata ttcatctctt caaaataacg ctgcgtgtaa tgaaaaacac cagagtttgc
    29821 ttctcttaca agccacaatt tgctttttgg gtccccagcg atgactagct ggtcttccat
    29881 caaagagtat ctttgtacgc cggaagtctc taatggcttt tcgataaaac caaaatcgga
    29941 ttcatgtttc gctacttgtt ctaatgattc atgagaattc gtgatttcga tctcaaaatg
    30001 cactttagga aaccttttga tcagatgttt catcaactga ggcaaaagat agacagcaaa
    30061 ggtgtgggaa gcaacgatcc gacatacttc ttcttcattt tgttcattga gcaatttttg
    30121 ctgagtttct tcccattctt ctaacaaaac cagtgtccgt tcatataata agtcggcttg
    30181 cttagttgga agcatctctt ttcttccgtt acgttgaaaa agatcgatat tcaattcccg
    30241 ctccaactgt ttgatttgat tcgagactgc tggttgagat aaaaacagta cttctgctgc
    30301 tctggaaaaa tttcttgttt catatactgc tcgaaaagtc gttaataact taaacatggc
    30361 accacttcca taacttttta tgataattac tataaaaact atttgattta aatatacaaa
    30421 aaaccatcta taatagaaat gtacaacaaa acagttgctg atccaactag taacttagat
    30481 gaagacaatc agctgtctgc ttgctcgtaa tcatacattc ctattcattt tacaatgtat
    30541 tcgttcatct tggtactaaa atctattaaa taaaaaggag aatattatat atgaagaact
    30601 ttttgaaaat tattccgatc ccgatctgcg ggttgatcct tgggttagct tcccttggaa
    30661 atctgtttaa agactatcac catgttgctt taggcaatct aactggcggt atctctatga
    30721 ttcttatgat attcattttt gttaaattgt ttatcttatt cgaacacacg aaacaaacgt
    30781 tgcatgatcc aatcatcgcc tccgtttcac caacttttac gatgtcactc atggttattt
    30841 gtacttattt tgtttcatat gaaagtattg caccaattgt caaattcatt tggctgtgcg
    30901 ctgttatctt ccaagtgatc cttgtctttt atttcaatta tcaccatgtt atcaaagctg
    30961 atctttcaat tgaagcaatt tatccaagct ggtttatcat ttacgttgga tttggggtca
    31021 tcactgtaac tgccgggaac ttcttcccac ttattggaaa aattttcttt tggatttcat
    31081 tgatctgcta tgcgttctta ttgccgatca tcatcaaacg tgtctttttc gtaagaaata
    31141 tggctcatcc gacacgtccg ctgattacga tcgttgcagc acctggttct ctttgcctaa
    31201 caggttatct taaaaacttt gctgaaccta atttttatct tgtcgtagca ttgttcgctt
    31261 tatctcaagt attgtatttc gttgtgctgt ctatgcttcc acgtttgttc aagcttcgtt
    31321 tttatccaag ctatgcagcc tttacattcc cgcttgttat ctctgcaaca gctatattta
    31381 ctactgttca ttacttcaac agtttaggta tctatagtag cgtattggat ttcttaaaag
    31441 ttgcggaatg tctgtttgca agtgtagttg tcttctacgt tcttgctcat tacgtacgtt
    31501 tcttaattaa agaacatagc aaacatggaa aatttgtttc taaaaaaatc gcttaataat
    31561 cgattcaata ggttgtgaaa aagctgttct gcatcaagta ataagaacgg ccaaacaaaa
    31621 acagctgttc atgtttttgt actggttggg cttattaccg cagatgcaag cttttgaaca
    31681 ccgtttacca agaggtcgta aaagaagtgg tcagcttcaa gaaataagaa ggaatttacg
    31741 aaaattgctt ctcaaatttt tgtgaaattt cggcttattt ccgtagaagc tacttctgga
    31801 acaccgttta ttcgaaacaa gaggtcgtac aagacttttg tcacgacctc ttgttctttt
    31861 gtcaaaatac tggtttagtg ctactatgtt cgagtatgct tttagtattt attgacgaaa
    31921 ggaaccgatc aaatgaaatc attgtcacat ccaataaaat ctgtaaagga ttcagcggtc
    31981 cgcgaaatct tacccggttt actgctttct tttgccatcg ctgccttcag caggcttctt
    32041 gctatttatc tgccacagtt aggtggagcg actattgcta ttttattggg tattttatta
    32101 ggaaatactt tttttaaaca gcctgttcta gcaatcggca caaaatttgc tgaaagcaaa
    32161 ctattagaat tctctgtttt tttactaggt gcaactgtca cagctcaaac gattgctcaa
    32221 attggtgtaa aaggaattgt ttttgtgtta ctgcaaatgt cagtcactat catcggtgct
    32281 tacttgattg gtaaaaaact gcaattcagc gatacttctt ctttgatgat ggctggagga
    32341 aacgctgttt gtggttcttc tgctattgct tcaatcgccc cttccatcca tgcgaaagag
    32401 gaagaaaaag gacagatcat cacactagtc aatctactgg gtactgtgat gatgttgact
    32461 ctcccttttc ttggattaca gttgtttggt ggaaatctca ttgaaaaaag tgcacttttg
    32521 ggtggtacac tccaatctgt tggtcaagta gtcgctgggg caagcttact tgacaattct
    32581 gttgtcgaat tcgccatgct atttaagatt ctacgtatta tgatgctcgt tgttgttgtc
    32641 tatctgtttg aaaaattcat gcacaagaaa caaccagctc tgtttgaaac gccaactgat
    32701 acgcagaaaa aacgaaaatt accgcttcct tggtatgtat tagggtttgt gatcttttgt
    32761 ttggtcaaca gcttgttttc tttacctgta gttattgacc aatcggcaca ttttatcagc
    32821 agctggtttg agatcactgc tttagcggca attggtctgc gtttagactt tgcaaaattc
    32881 atgaaagaag gtacacgctt cttatcttat ggattagctg tcgggcttct ccaaacagtg
    32941 atggctttac tatttatttg gctgatcaaa gtttagaatg aacatgaaaa aacagttgtg
    33001 attctcgcat ggaacaatgt ccgaatcaca actgttttta attttgcaga tatccttcta
    33061 acaacaacaa ttggcgtttg atttcaaccc catctattgc gtttcccata tatcctgtca
    33121 gttttccatt ttgcccgatc actcgatgac aaggaataac gattggcaat ggattggttc
    33181 gatttgcctg cccaatcgct cgcacagctt tcggactatc aacagcaacg gctacttctt
    33241 tatacgttcg gatttctcca taaggaatgg tcgacaaagc tttccagaca ttttgctgaa
    33301 aaggcgtacc tctctttata gataaaggaa catcaaattc ttttcttttt cccgcaaaat
    33361 attcttcaaa ctgttcttgt gtgcgcgtta aaattcgctg attcgctgat tcgctttctg
    33421 aaattggctg aaaagataca gcagtcaatc cggattcatc tgcatctaac cataaaaagc
    33481 cgataggtgt gtgtattttc attttgtttg ctcctagtca atagactgga acttcctcct
    33541 ttttctctat ccaaaactac ttgccaacta ataaaaattg ttcttaaaag tttgataaac
    33601 cgaggaaaca tggtaaacta aatacgttag tttacaaaaa gaatacctgt tcttttacgt
    33661 aaaaccaaat tttaaaggtg ttatttaagg tttattaaat tttgaatgta aaccatatat
    33721 aaacatacga ttgaaaggat agaaacgatg aaagtatcaa ttgttgtccc atgttttaat
    33781 gaagaaaaaa ctgttcctct tttttttgat gaggttgaaa aattcaaagg tgcccatgag
    33841 ttcgaatatt tgtttattga cgacggatca aaagacggta cgttagcagc aatcaagaag
    33901 ctagcctcag cttatcctta tgtccgcttt gtctcatttt ccagaaattt tgggaaagaa
    33961 gcggctctgt acgctggatt gcaagcttct acaggagatt tagtgaccgt gatggatgtt
    34021 gatctgcaag atccgcccga actgcttcct gagatgatca accggatcac tacttctgat
    34081 atcgattgtg tcggcacccg gcgttcgact cgtgaaggcg agcctgcgat acgcagtttc
    34141 tttgcgaaaa aattctacca gttgatcaat aaaatcagcg atacagaaat ggtcgatggc
    34201 gctcgtgatt tccgcttgat gactcgtcaa atggtggatg ccgtcctaga attaacagaa
    34261 tacaaccgat tttcaaaagg attatttagc tgggtcggat ttaaaacaga atatatttct
    34321 tatgaaaacc gcgaacgtgt ggctggcgaa acatcttggt ccttctggaa attattcaac
    34381 tactcgattg atggtatcgt gaacttctcg gatgcaccgt taacgattgc ttcatttatc
    34441 ggcgctttgt cttgtgtcgg ttctggtatt gcaattttgt tcattatttt gcgggcattg
    34501 ttgtttggtg atcctacatc aggatggcct tccatggtct cgatcttcct gttcattggt
    34561 ggaatccaat tgttatgtct tggaatcatc gggaaatata ttgggaaaat cttcttagaa
    34621 acgaaacagc gtcctgttta tatcataaga gaaacagaaa aagtgaagcc aaatgaattg
    34681 aagcctcacc aataatatac gtcaaatgaa gtaaacattt ttttaaatga aaaaggactg
    34741 tgacaaaagt cttgtcacag tccttttttc cgaataaatg gtgttccaga agtagcttct
    34801 tcggaaataa gccgaaattt cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta
    34861 tttcttgaag ctgaccactt ctatcacaac ctctttcata tcttattact tcttcattgc
    34921 tgcttttagt gctgctgcta atgggctttc ttctggttct tctgcttgtg catatttttt
    34981 cagcaagcgt cgttcttcat gtttggtcat tttcttcttc tgctcttttt tatcaagcaa
    35041 tttttctgtc gttccatcgt atttacaacg gaaataagca ccgtttttac cttcgatgat
    35101 ttccattttc cggtgacatt gcgggcatcg atgattagag acttttggat cttttcttct
    35161 gcggtagttg caatctgaat tggtgcagac atagattttg ccgtcacgtg tgtttttctc
    35221 gcgcagattg gccccacatt ctgggcattt cttttgggta atcgagaaat cttgatattc
    35281 ttgtttgctt gatttgatct cattcaccaa gcgttttgtt tctttttcga ttccattcaa
    35341 gaaggttcct gcttgtttct ttcctgcagc aatagcttca aggtcttttt cccagctttc
    35401 tgtcaaatct ggtgttacta aagagggatt taccaatact aataattgct tacctttagg
    35461 agatacttgc agtccttgag gggtacgttc catcagttca gatttgatta gtttctcgat
    35521 aatctctgca cgcgtagctg gggttcccag actgtatttt tccatttgac caagcaagct
    35581 tgcttctgac aatggtttag gcggtgcaga tagttctttt ttgatggcga aatcgggatt
    35641 gattctcatt cctttttccc atttgactgt ttccgtctca gtttcagttt cgcctttcca
    35701 tcctgcttgg acgacacgat tttgccgaaa aatgaatcgt tctttaccaa attcaaccgt
    35761 aacttttgtt tggctgactt tatggggttg agcaaacagt cctaaaaaac gagagacaat
    35821 catttgatag atttttcgct cttcattact taatttttca taacgtgctc tgttttcagt
    35881 tggcaaaagt gcatggtgat cggtcacttt actgtcttgg aatacttttg tctgttggac
    35941 gatggctccg tttttcaaat actgcttgac ctcaggagca aagtcgctga ctgcttgcaa
    36001 tcgttctttc atcgtacttt tcatgtcatt tgtcagatat ttgctgtctg tacgcggata
    36061 agtaacgatc ttatgggtct catacaaact ttgtacaaga cttaatgttt tctttgcaga
    36121 atagccatag cgttgattgg cttctctttg gatctctgtt aaatcataag gcaatggcgc
    36181 agattccgtt tttaattttt cttggacttc tgtgacagtt cctttgcttg tactgatcaa
    36241 ttgaacaagt ttttgtgctt cttcttgtgt ttttaaagca aattgatttt tttgaagcat
    36301 tttggcttgt tcttttccag cttgcaacgt gatcgtgaaa tacgtttgtg gacggaactg
    36361 ctcgattttc ttttcttggt ctctcactaa tgctagtgtc ggtgtctgca cacggcctgc
    36421 tgataaatta tccgcgtact tcacagttaa ggctctggtc acattcaatc ctaccagcca
    36481 gtccgctttt gcccgggcaa gagcagattc atacaatgca tcatattctt tcgcaggtct
    36541 tagttgcttg aatccagttt tgattgcttt atctgtttga gaagagatcc ataaccgctt
    36601 cactggttta ttgaaatgca catactctaa gatccagcga gcaacaagtt ccccttctct
    36661 tccggcatct gtcgcaatga ctgcttctga gacatctttt cgctgagcca gctgcttgat
    36721 tgcttttaat tgatgtcctg ttttaggaag tggtttgatt cctagttttt tagggatcat
    36781 tggcaacgtg tccatttgcc agctctgcca ttccttattc agatcttccg gcattttcaa
    36841 tcctaataaa tgaccaaggg cccaagtaac gatcaccttg ggtccttcat aataattttt
    36901 gtgcttttga ttagcaccaa gcaccttgct taggtctttc gcaacgcttg gtttttccgc
    36961 tagaattagt tgtttcataa aattcctttc tgctttctac tgtgatgggc tgatgatttt
    37021 tcaacaacgc aagtcccttg tattgctgta aatcatcgtc acactcttca caccagcgct
    37081 catctccgtc attccaaaag gcgctgagaa agttactttt tgcattgata tattcataat
    37141 tttgatggat ttcgttccat tgtttttcgt acgcttgtcg tgcttcttcg aaagactcga
    37201 atgtttcttc ttcttcaata tcttcttccc agtcttcaaa aaaccaccaa ggctcattgt
    37261 ccccgaacat ggtaattact tgatacatac tcgtgctcct tctttcagct tttctagact
    37321 catcttagtc acttttcaag aaagttgcaa gaattttctc ttataaaagg gtcatttaat
    37381 gaacatctta aaattgttac aaatgaatct gattgtttaa ggcaaagtag cgataaagtt
    37441 attgatcatc caaaaatagc tgtcttctgg atcgattgtt ggttcttttg taaagtatcc
    37501 taaaaaacgt aaggaaagat aaccgtggat catactacga agttccctgc ttcgatgaac
    37561 tttttcttct ttcgttaatt gaaaagcctc aaatatctgc aaaatgatct gattcgtttc
    37621 atggattcca tctaataaaa cagcttcatt ggtatgcggt acactgatca gtaactcata
    37681 cactgcttga tttgaaaaag caaaggatcg ataagtttct gcatagatct gcagtgcttc
    37741 ctgaccactt tttcccacaa gagcccgtcg cagctggtca ttaagttcct gcagaagttt
    37801 cgctgttagc gctgttttga catcacgaat atttttgaaa tggttataca gcgagggagc
    37861 cttgatatcc aagcgtttgg ctaaatgctg aaaggttatt tgctgaacat ccatctcttc
    37921 tgctagttct cgaaagcagt cgatgatttt ctcttgagac agatttcttt ttttcatttc
    37981 catgtttcct ctcataaaat aaatccttca ctctttcagt ttaccacgaa aaagaaagag
    38041 aaaaaagaga ctttcattga taaactacat attgtagttt ataataaaga gctgtagatg
    38101 aaaattttca gaaagaagga gtctaattgg ctgcaagaat aaaggaaaaa ttatttagga
    38161 aaagagaaac ttgggaacaa aacttgtttg tcttatggtt tggaacattt atggctggta
    38221 tcggatttag tttagtcatg ccttttatgt cactttacat agatacgtta ggtaattata
    38281 gtacgtcaca attgaacttt tggagcggat tgactttttc atctactttc ttggtgacta
    38341 cgcttatctc tccttggtgg ggaaaattag ccgaccaaaa aggaagaaaa ttgatgctgt
    38401 tgcgtgcttc attaggaatg gcaatcgtca tttctttgat gggttgcgtg actagtgtgt
    38461 atcaattagt cgcattgcgt ctgcttcaag ggattttttc tggttatatc agtaatgcca
    38521 ctgcccttgt cgcaactggg acgccaaaag aaaaaagtgg tcaagttctt tgaaccttag
    38581 caactggttc tgttacagga acattacttg gaccgcttct tggcgggatc tctgcttcag
    38641 cgtttggcta tcgccctacc tttttcatta cagggatcat cttatttttc gtcttcttgt
    38701 tgagtctgtt tttcgtccat gaaaaatttg tcccagtgaa aaaagaaaac atggtttctg
    38761 ccaaacagat tttcaaagac ttgaaatatc cacacgtcgt catcggtatg ttcgtcacaa
    38821 cgatgatcat ccaagcatcc aacaattcca ttagtccaat catcagtctg tacattcgcc
    38881 aattgctgca tggacatggg aatgttacgt tgatcagcgg aatcattgct tcgatacctg
    38941 ggattgccac tttgatagcc gctccaagat ttggtcgctt aggtgacaag attgggagtg
    39001 aaagaatact tgcgattggt ttgggttttg cgattctggt ttatatccca atggcattcg
    39061 tcacgaatgt ttggcaatta gctgccttac gttttttgat tggtatctct gatgcttgtc
    39121 tgctccctgc tgtccaagca ttgatcacaa aatattcgcc tcatcaagct gctggacgga
    39181 tcttcagtta taaccaatct ttccaagcaa ctggtaacgt agtagggcct atgatcggct
    39241 ccagtgtttc cagtgcattt ggttatcgcg gcgtctttct ttcgacttct cttcttgttt
    39301 tggtcaactt cttgcttgta cgacacaata cacaagagat ccatcagcaa gaaactgcag
    39361 aagaaaaagc ttcaggagtc tccatagctt cagggcactc tccttctcat agacaataag
    39421 cgtaaatgaa aaacactgct tgaacagttg tcacagactg tccttgcagt gtttttgttt
    39481 aggcctgttt tttcaagaac tcagataaga ggaataatcc cttttccaag gtttcttttt
    39541 tacaacagta acctagtcgg gcatgcttcg gtaaatcaaa ggcacttcct ggtactaata
    39601 gtactcctgt ttcttctaat aaattgatac aaaatgtctg gtcatcttct tcgatggcta
    39661 atttaatgaa agaagtagag acgtaattgg gagcgacaag ttcaactttc ggttcattgg
    39721 ctacccattg ttttaaaatc acaaggttct ctgtaatgat ctttttgttt cgcgccaata
    39781 ttttttcttt attcttcaat gcatgtacag caagatcatc tgacaggact ccgccgcaaa
    39841 tcatcgtgta gtctcgatac tttcgaaaaa cttctgccaa ctcaggtcca gtagctgtcc
    39901 agccaatacg aatacctgga atggagtagg ttttggatag ggaattggtt gctatacctt
    39961 tttcatagac atctacgatc gacaagaatt cacctttatc cgttaaaggc gcatatactt
    40021 catctatcaa tacatatgca tctactgtac gagcaatttc tgcgattttt tctaaagttt
    40081 tccgatctag caatgtgcct gtaggattgt ttgcactatt caaacagatg atttttgttt
    40141 caggtttgac taaggcttct agctgttcta aatcaaactg ccaatcttct tcttccttca
    40201 aatgaacaaa gtcaacagcg gcacctaaag atttggggat gtcatataat tgctgataag
    40261 taggaagcat cgaaacaaca tggtctcctg cttccactaa cgcgtataga gctaaaagat
    40321 tagcaccagt cgctccattc gtttgtaaga tgttttccgg atcgacggtt tgataaagag
    40381 aagcgacttc ttgtttgaaa acatctgatc cttcgatcca gccatagtcc aatggttcgt
    40441 ttttgcgttt ttcgaaaaat ttacttacgc tagtcccatc taagccaacg agttcgtcta
    40501 atgtcaatgc ttcgatcgag ctttgtgcaa tatcatatac cgctttcttt tcccatttat
    40561 ttagccattc ttctacaccg aaatttgcga ttttcatgtt tctttccccc ttatatcttg
    40621 atccagccta cagcaagcaa cccgattgaa agcagtgcgg ccacacaaat cagcccaatc
    40681 attacttttt cttttcccgt aatttgatgt ttggtttcac gacaagcgat ccagtagaag
    40741 caaaaaccag gaagataggc aatactacat aaaaggacat aagaaaatcc tgctaaaatg
    40801 atgcccatca attcaaaaac tacagcaatc ccaccgatca ctacttgttt tttgattcct
    40861 tcttttaaag aaaactggac ttgatagatc ccgacaagta gataacaaat caaaattgcc
    40921 gctgtacata aagaataggc aaattgatag gcataatcag taactaagaa agtcaataag
    40981 aatagattag tcaagattgc agtaacaact aacgaataag ttggggcatt ttttttattc
    41041 gtttttcccc aacctttcgg caacagaccg tctcgagcca tcaacatcgt tgtttctgct
    41101 gggagcatcg tccaagacaa ccaagaacca atgatcgaaa taatcaagcc gatattgatc
    41161 agcgctgctc cccaatttcc cacgattcct tttaaaatat ttgccatagc aggttgtgaa
    41221 atagttgcta actcttcttg agataagacg ccgtatggca agatcgaagc aaggatatag
    41281 ataaacaata aacaaagcag accaatgatc gatgcttgct gggcttctga acgtttcttc
    41341 gcgcggttgg ctaaaacact tgcgccttcg atccctacaa agacccacat cataaccatc
    41401 aaacaacctt taatctgcaa ccagatcgtt ccttgctgcc ctgttccatt cactagatta
    41461 tcagatacgt taccccaaaa atcagcagtg aatatattca ctttaaatgc gataaataca
    41521 aatattaaaa ataggaataa tggcactaat ttacagatcg tcacgattgt attgatgacg
    41581 ctcgctcctt caactccatt gtttactaat aaagtcagac cccaaataaa aatacttgca
    41641 aataaaatag aaggaacatt ttgtccacct ttgaatatcg ggaaaaattc tcccatggcg
    41701 ctcatcagca tcgttgcaaa agcaacattt cccagccacg cagacaacca gtaaccccaa
    41761 ccactgatga agccacctaa tttaccaaat gaagcttccg cgtaaccgaa aataccacca
    41821 tctagatctg gacgcttttc tcctaaatga ttcagcgata ataccaaagc taaaatacca
    41881 atcccaacga ttatccatga aaggatcgct ggccctggag ctgcagaact agccagatca
    41941 cttgtgatcc caaacacacc gcttccgatt gacgaactga caaccagagc aaccagtccc
    42001 catttgctaa tacccgtcga tttcttttcc atttccatca ccctcactta taaaattgaa
    42061 caaatcactt gcaaaagatt cccttctatt ttgcaactgc tcttgcttaa caaaaagcaa
    42121 ctttattcat attattggat attatgaact ttttacacaa gtatttcaag tataaaatga
    42181 ataaaaatta attttattgc aaaaaaacag ctatgctatc caaaagaaaa ggtgtttctt
    42241 ttgttagcca tagctgtttt gattcattta atatcgctta aacgtattgt aaaacttttg
    42301 tttcttgata tgcgtggtcg atcaaaccac cacccagaca ttccattcca tcataaaata
    42361 caactgcttg tccaggagtg attgcacgta caggttcgtc gaatacgact tctgcacgat
    42421 tatcatccaa taagcgaact gtcacaggaa cgtcttgctg acgataacgg aatttcgctg
    42481 tacacttgaa ttcttttggc attggttcat tcgtcgtaaa gtggatttca cttgcatcca
    42541 agcttgtggc atacaatgca ggatgatgga acccttgccc cacatataat gtgtttgtcg
    42601 ctagatcttt tcctacaaca aaccagggtt cttgcgaatc tcctccgcca ccgatcccta
    42661 gtccttgacg ttgaccaatc gtataataca tcaagccagc atgttgtcct ttgacttcac
    42721 catcaagagt gaccatattt cctttttttg ctggcagata gttgctcaaa aattgtttga
    42781 agttcttttc accaataaag cagattcccg ttgaatcttt tttcttagca gtcgcaaggc
    42841 ctgcgcgttc agcaattgcc cgtacttctg atttttccat gccgcctaga gggaacatcg
    42901 tttttgaaag ctgttcttgt gataattggc taaggaagta agtttgatct ttattattgt
    42961 ctactccacg aagcatgtgg accacgccgt tttcatcgcg agtgacttgc gcataatgtc
    43021 cagtagctac ataatcggcg ccaagatcca ttgcataatc caagaatgct ttgaatttga
    43081 tttctttgtt gcacatcacg tctgggttag gtgtacgtcc tgcgcggtat tcggctagga
    43141 aatattcaaa tacacgatcc caatattctt tttcaaaatt cacagagtaa taaggaatcc
    43201 cgatttgagc agctactttc gctacatcct tataatcttc cgtggccgtg catacaccgt
    43261 tttcgtccgt gtcatcccaa tttttcatga atattccgat cacgtcgtat ccttgttcct
    43321 ttaacaaaag tgctgttaca gatgagtcga caccgccgct catccctacg acaacgcgtg
    43381 ttttgctgtt gtctgtcatg atatcaccat catttcaatt agattctgaa acatctacga
    43441 gttgaaggtc gcaacttttc agtattttac attatacgtc atatttctga aaatttcatc
    43501 ccttaattat caagtcgtaa aaatactgtt ctactggacc tattcagtac acaaacatga
    43561 tagtcaacca aaaaatcact catcttttga gcattcgata taaaaaattg gcatactcat
    43621 aaaaagggcc agcctttcgg caataaggaa taattttata aattgtgaaa aactattttt
    43681 caaaattgct tcttatcgct taaagctgca cgtctcattt acaacctttt ctttttagat
    43741 gttatgagtt gctcagataa gtgattttgt ttactctaag aaaaaaggga ggcaaaagat
    43801 acatgaatta ctttatcagt gatatgcact ttttccatga acgcttatta ggaaaaaacg
    43861 actttgctcc gcggctcttt gcaaatgtgg atgaaatgaa ccaacagttg atcactagct
    43921 ggaatgcagt aataaaagaa accgaccatg tttatcatct aggcgacctt gctatgcatc
    43981 cgcaatatga aaaagcaaat gcagaaattt tggaactggt cactcaactg caaggaacaa
    44041 tccacttgat caaagggaac catgacagtc gcgctttttt ccgttattta gaaaaaaatg
    44101 atcctaggct tactgacgga agtcccaaat tttattttta tgatgtaggt gccatcgtta
    44161 aattcgacca tcaacaatat tacttgactc attatccgat gttgctcggg cctaacgaca
    44221 ctatccgcaa tcttcatgga catatccatc attacagcgt acctatcgag aacgatgtca
    44281 atgtaggcgt agacgcaccg gaaagagaac tcttaaagga aaaactacca tttggcactc
    44341 cgttatctga gcaacaaatc aaagaaatct atgaagcaaa gaggaagtac gataggtcgt
    44401 gaaaaaactg ccctatatca agtattaaac aaataaagat aattactcat atttttcatt
    44461 tgagtaggct tattgccaca ggaacattag gatcagaaat ggagttgtaa caagacttct
    44521 gtcacaactc cattccatac tattcgtctt tttgctcatg gtgttctttt accagttttt
    44581 tctgtaattc tttatcataa tttcgggcag ctgacgttaa aaataaaacg attccgcata
    44641 ataaaatccc gattccggaa aacaagaaca ttttttctac ccctaaacga tcagctaatg
    44701 gtcctgcaaa taataatcca attggacctg ccaggttcat caaagagttg aagttcccta
    44761 agacacgacc taaaacattc gattcataac tctgctggat cattgccatc agcagtgtat
    44821 tgaaatatgg cgttgcacaa cctgctcctg cattcaagat aagaaagtac aagaaccctt
    44881 gactatcgtt tggaaccaat ccgcttgcac cgatcgtcag gcccataatg atgtaggcta
    44941 taataatagg tttcattcgg tctttccatg ttcctataaa actgatcagt gcaccgccta
    45001 agagcattcc gactgcgtag actacttcca ctgctcccgc ctgtccaacg gaaccaccaa
    45061 agtaatctag cgtcatcaat ggatacagac tgattgctgg catgaacaac agcatcacaa
    45121 atgcaccatt gatcgttatg taccataacc ctttattatc catcaattgc tgcagcccaa
    45181 attttgcatc tttaaaaata gtaagcggaa cttccaatat ttcaggagaa acttttggga
    45241 tcttcacaaa aatcaataag ccgacaccaa aaactgcccc caatacatct aataaaataa
    45301 ggtaattgac aggaacgact gaaaacaaag ccgcacctag agctggcgca atgatgaaat
    45361 tcgctgattg gaccattcct aactgtccat tcgtccttgt tatgtgagaa gatggaacga
    45421 ttgtcggcat aatcgactgg atcgttggca tttggaatgt ttgtgcgacc gaacgcataa
    45481 acaaagacac aaaaactagc caaatcggga aggattctga aatcgttccg actactgcta
    45541 aaataagcgc gaaaatcgct acaataatat ccgtgacaat caaaagagct tttttatccc
    45601 agcgatccac tagcggtcct acaaatgggc ttagtaaaac catgggaatc attcctagta
    45661 aagtagcaaa actcaaaatt gtcgccgaac cggtttctct tgtcaaatac cagatgatcg
    45721 catattggac gaccatacta gtgattcctg acaaaaactg tcccgataaa aagaacaaat
    45781 aatttctctt ccaattcgtt aaaacgaccg actgttcatt ttccataaac atccctcttt
    45841 tctttttcat tttgtatagc gtttgcacaa caatcattat acgggattct tttttcaaaa
    45901 aatacctttt tccctaaata acaatcgttt caatttaaat ttctttactt agaaacaata
    45961 aatcagcaaa atattaaaaa taacaacatg tgttaaattt aaaaataaaa aagacaacta
    46021 atttgcgtcg tccttacaaa gttagtcatc taacaatagg ccgtttcaaa actagttgtc
    46081 tttttatatc cacagatcat tcgacttttt cgaataaccc acggctaatc atcccatcca
    46141 tcagaaaata aaatttatct tggctcattt tctgttttgg atctttgaaa atattgactg
    46201 ctttcaatcc atttgctgta gtgatcgaca ttttcaatgt ttgaatatct tttgatacag
    46261 tgatttttaa tttgaatcct tctttgcttt gcggcgtggc atctaatggt cggatcaatt
    46321 gataattgcc tccgtttgtt tctgtaaatc cattatcgcg caatgtatat ctttttgcat
    46381 caggatgcaa acgatagcta attggtgcgc ccagtaccgt tgctgttgtt tcaaatgcca
    46441 tgattttcac ctcgataaaa ttagtttctc tttattataa aagtttatcc tgatttttac
    46501 taggtttttc agttaagttc cctaaaaaat gaaggcgttc tctcagacat ctgttcatat
    46561 aagaatgact tcactagtta ttttttgaca gcacgaacta attgatcaag gaaatactgg
    46621 atgtcgtctt cggtatttcc caaaccaaag ctgatacgga tggattcttt tagtatcggt
    46681 gtatcttctc catacattgc ttctaatacg tgtgaaggct cgatatttcc tgctgtacaa
    46741 gctgaaccag tagaaatagc gaccccatta agatcaagct tcatcagtag caaatcattt
    46801 gcttcgccaa ttaagcgaag attcaacaca tgagggagtt tattcgttaa atcaccattt
    46861 acttgataag ggatttcgtt tgcttctaat gtatcaagaa tcaattgctg gaatccttta
    46921 tatttgttgt tacgtgcttc acgttcttca ggtgacagca gttcaactgc ttttgccata
    46981 ccccagatcc ctgctaaatt ttctgtacct gcacgccgct tttcttcctg ctccccgcct
    47041 aaaagcaatg cagggatatt tactccgtct cgtttatata agaaaccgac gccttttggc
    47101 ccattgattt tatgagcaga gatacttaat aaatcgattc ctaattcttc tggatggatc
    47161 gtttggtttc cgtacgcttg taccgcatct gtatggaaat aagctggatg atccgctaaa
    47221 agttcaccga tttctttgat tggcatcaaa tttccgattt cattgttccc gtacatcaca
    47281 gagaccaaga tcgtatcttc tcgtaacgct tttttgacct cttcgatcga cagattccct
    47341 ttttcatcta caggcagata tgtaacgtca aacccctttg tctctaaata tttcatggta
    47401 ttaagaacag caggatgttc gatagctgta gtgattagat gtctgccttc tttttgacgc
    47461 gagaacgctg tttctaagat agctgtatta tccccttctg ttcccccact gttaaagatg
    47521 atctcgtgag gttttgcttg cagactgtct gcaatgattt gtcttgctgt ttccaatttt
    47581 tcatgcgcgt ggcgaccaaa tccatgtatg cttgaaggat ttccaaacgt gtcttgcatg
    47641 attgccgtca tcgcttcaat tacttgtgga tgcatggggg tagttgccgc atgatccaga
    47701 taaatagttt tcacttcatt catcccttcc aaattattgc taggcacatt ctagcataaa
    47761 atagcgaaca aaccaaagaa gagctcattt ttgtctagag gacgtttcat gggcatagaa
    47821 aaaaggagct gtgacaaaag tcttgtcgca gctccttgaa gctgctcact tctgtcacaa
    47881 cttctttccc tctttttttt gtttattcga attctttttc ttccagacgg aataacggac
    47941 tcatcggtgt attttcatgg atgcgtttga ccgcttcacc cattaattct gaacatgtaa
    48001 cgattgttaa gttttctgga tgacgatttt cttctgtgaa aacagaatca gtgatacaaa
    48061 tatctttgat tggtgcattg tctaaaatac ttttcgcatg atcggacaat aatccatgtg
    48121 acgcacaggc atatacttct tttgcgccgt tttcttttaa tacatttgct gctcgtgcaa
    48181 gcgttgctcc tgtgttcaag atatcatcta ccatgataca tgtctttcct tgaacatcac
    48241 cgatcacata accaccctca gttgaagcat catctgcatg atctacgatg gctaatgtgc
    48301 tgttcaaata ttctgaaaga ctacgtgcac gctgaacacc gctgtttttt ggcgaaacaa
    48361 ctacgatgtc atctcccgtc agacctaatt gacggtaata atgagcaaac aacggcatcg
    48421 taaataaatt atctacagga atatcaaaga atccttgtac ttgaaccgtg tgcagatcta
    48481 aagtcagtac tcgagttgcc cctgcttctt cgatcaagtt agcgatcaat ttcgcagtga
    48541 tcggttcatg aggtttcgcc gtgcggtctt gcctagcata cccatagtaa ggcaatacga
    48601 cattgatcgt tttcgcactt gctcgcttca ttgcatcgat catgatcagt agttccatat
    48661 agtaatcatt gacaggtgca ttcgttgatt gaacgatata cacatgatcc cctcgaatac
    48721 tttcttcgat attgatggaa atctcgccat cactaaattg cttcacgacg cttttaccta
    48781 attctgttcc gaacactttc gcgatttttt cagctaatgg acggttgcca ttcaaactaa
    48841 aaatcttcaa cgtgtcatcc tgatacttat tactcatgta gtcctcccca aaacttctct
    48901 attcttcgat agtttaacat atttttccaa gattttctgt taatttcagc aaattcttaa
    48961 ggaagtgaaa ctgttccgtt actccaactg gcaggaatga ctttatgata atcgccccag
    49021 ttgtcgtgta ctttgtaata acgttctcct gtttcagttt caaaataagc gtacgctgtc
    49081 acccaatgat tgccatatgt actgccaagt actttcaaaa taccaatcat cacaggtttg
    49141 ccttcacgta tacgttttgt agctcgctgc catgaaccta gaagagttgc tcgtgcacgc
    49201 tcatgattgc cattcttctt taaaaaacta gaaatccctg tactaacctg aaaaggaatc
    49261 gtaggaagtc ctagtggttg gatctggcta cgcaatgcct tcgtcaaaac caacgaatca
    49321 gccgtatttt tcttgcgaat ctcatttgga agcatccacc ccttacgata gtcttgataa
    49381 taggcaagga gaactgctgc cgcgtaagta ccacaaagat agcctgaggg ggtagcccat
    49441 ttcttatacc tcaaaaaacg ttcttctggc aatcccaccc agcaatttgc gagttccttt
    49501 cctgaccttt tttcttcgat caaaacggga atatcttctt ttctgttgat ccatctagat
    49561 aactcgattg gctcttcatt gatcagcttc cattttttac aagaaagacc ctcttgataa
    49621 gtatattttg ctggagaata ttctaccgga tgtcctgatt gatcgataat cacataactt
    49681 tgatcttcaa aaaccagagc taaaaaaagc aatttacttt ttcttgactg cagctgctga
    49741 gtgctggcta attttttaat ttttttatct actttgaaat ataaaggttt atccactact
    49801 tgattcagtt gggatcgcct cctttttttc attataagtg aaggattgaa aaaaacaaat
    49861 cagtttgatt gttcgaattt tcaatttttt atgttctatt tgttaattcc ttcattttgt
    49921 aagataatgg gcacacgaaa caaaaaagga gcgatcgatg aatgaacaaa gaacgattga
    49981 aacaagattt ctatgaagca gtcaatgaag aatggcaaaa aacagcaaaa atccctgcag
    50041 ataaaccagc tacaggcggt ttccaagatt tagttgacgg gatcgaccaa cttttgatgg
    50101 gagaaacaga cgatctgctt gcccatccag aaaaagtgtc ttcagaaaaa atgaaacatt
    50161 tccttgcata ttatcaactg gcaaacgatt atgaccgaag aaatgagttg catgctcaac
    50221 cgcttcaacc tataatcgaa aaagttgaat cattgcagtc tttcgacgaa ttgaataagc
    50281 aatttccaga atggctgttg gatacattac cattgccatt taatctggat gtagatgcag
    50341 atatgaagaa tgcgcaaacg aacgccttct ttgcttatcc gccttcttta ttccttccag
    50401 ataagacgta ttatgcatca gaccatccca acggtgcaca actaataggc gttttctttg
    50461 atatggctgt tcagttatta gagcttgccg gcaaagaaaa aagtcaggca gaagcaatca
    50521 cggaacaagc agtgcaattt gataaattga ttgctcctca tgtaaaaagt gcagaagaaa
    50581 acgcagatta cagcaaaatg tataatccgc aatcttttga tacatttatt aaatacacag
    50641 ataaactgga cttgaaaaaa cttgtcacgg gtttgattgg gacaactcca gataaagtga
    50701 tcgtaactga tccagtgtac ttcgatcatt tgaatgacat cctgacagaa gctcattttc
    50761 cgttattcaa aagctggatg atcattaaaa cagtacgttc tcttagtggt tatctatcag
    50821 aagaattccg ccaagtcagc ggaatctatt ctcgcgtatt gtctggaaca gacgaagcaa
    50881 tgcctcagaa aaaagcagcg tattaccttg cctctggaca attcgatcaa gtagttggcg
    50941 attactacgg aaaaaaatat tttggcgaag ctgctaaaaa agacgtagaa caaatggtga
    51001 aaaaaatgat cgccgtttat aaaaaacgtc tagaagaaaa tgactggctt ggggatgcaa
    51061 caaaagaaaa agccgtcata aaattaaata acttaggtat ccaagtaggc taccccgata
    51121 agatcccagc attattcgac cagttcaaaa cagtacctgc agatcagggt ggtactttgc
    51181 tttcaaatac tcttgttttc agcaaactca cattaaaaga tcgtttcagc aaatggaaca
    51241 aaccagtgga tcgttcagaa tgggaaatga gtgctgacac ggtcaatgct tattatcatc
    51301 catttcggaa tgtgatcgtc tttccggcag cgatccttca agcaccattt tacagtctag
    51361 aacaatcaag ctctgctaat tatggtggaa tcggagctgt tatcgctcat gagatttccc
    51421 atgcatttga taataacggc gctttatttg acgaatatgg caacttgaac aactggtgga
    51481 cagaagaaga tcttacacaa tttcaaaaca aagcacaagc aatgatcgaa gaattcgatg
    51541 gtctgccttt tgctgacgga aaagtaaacg gaaaattaac cgtttcggaa aatattgctg
    51601 atgcaggcgg tctaagctgt gcattggaag cagcaaaaac ggaatcagat ctttctatcg
    51661 aggaattctt tgtcaattgg gcaacgattt ggcgtacaaa agcaaaaaaa gaataccaac
    51721 aacttttact gcaaatcgat gtccatgccc ctgctaaact ccgagcaaac gtgcaaccaa
    51781 aaaaccttga cgaattctat gaaacatttg gtatcacgga acaagacgct atgtatctag
    51841 cgccagaaaa aagagtgcga atctggtaag aggttgtgcc agaagtggtc agcttcaaga
    51901 aataagaagg aatttgggaa aatcgcttct caaatttttg tgacatttcg gcttattttt
    51961 cgaagaagtt acttctggaa taccatgtat tagaggttgt gaaaaagctg cttagtttca
    52021 agtagtaaga aaaattttgt gaaaatggct ttccacattt ttgcataaat ttgacttact
    52081 accgagaaac tggcttttga acaccgttta tcgagaggtt gtgaaatcag cgtcctgacc
    52141 tgagtattag agaaaaaaac ggaaaaatag ttcctcatat tttgccgttt gttgagctaa
    52201 taccgagggt cagctgattg aacaccgttt agtggagaga ttgtgaaaaa gctgtcctgc
    52261 atcaagtaat aagaacagcc aatcaaaagc agcaaggaga acctcatcta taaaaagata
    52321 gaaggaagtg agacatctat tggtctcatt tccttctatt tttactttcc tcagtcaatt
    52381 gttttacatc ataaaaggag aaattccact tatttttttc gctgtttcat gacataaaca
    52441 actacaccga ttaacaaaat tcctccagct aatgctagca acgggttatt aaattctccc
    52501 gtttttggga gtccttgagt gccatttttt tcactttctt ttgctgtcct atccggatga
    52561 gaaggttttc catttggttt tccatttggt gattcatttc ctggattttc tctttctggt
    52621 ttgtcatttc ccgggggcac atcggcagtt tctcgcacag tgatcgcttt ttgctgggta
    52681 acagattctg tatgagcaga ccatttttcc ccatcaaata tttccgaacg ataagtgact
    52741 tctagcgtat attctccttt tgaaggtgct gaaaaaatag cggtatgatt agaaaattga
    52801 gcacttgact gtgtttcaac gatttggatc ttttctggga aataacgcat atcttcaaag
    52861 acaggcgttc cttgatttgg tgtgaaagtt gacacaaaaa ctgtaaaagg cttcagttca
    52921 atgatatttc cttctgcatt ctccgccaca ccatttgctt cttgtataat aatcggatta
    52981 ttttgttcat ttggttttgt ctgatttggt tcttgtacga ttgcatactc tggatattcc
    53041 gaagacggtg aggtttctcc gtcaaaaata gcacctacag ataatggaag accatccact
    53101 ctttcctttt ctgtttgacg attccccggt ttaatctgca tcagtccatg aacatggata
    53161 cttccatttt catagcgcgt aacaggctgc tgaccttctt tcgaacatgt atttagagac
    53221 tcgatccaat cttttgatat ggtatgtcca agagtatttt ctgattgttt ctctcgttca
    53281 ttccaatagt agttcaacga gtggtagatt tgatcttcgt tttgattcaa tgggcggatc
    53341 tgctctctca tctcaagatt ctctgttcgg aaagataaga taccagaagc atggtagtct
    53401 gtgtttcctg ctgaagaggt ataaagagcc acattatatg aaccgttatt aaaacttgta
    53461 ctgctattga cgataatatc tgttccgcta ttggaatcga ttcctttcgc aagattttcg
    53521 aaagacaaac tattgatcaa ttcatgaggg cccgttaaag aagaaccgcc cattttgaat
    53581 ccattaccgt taccttctcc ctcaattcct ggtacccagc cgtttcgata agcaactgag
    53641 tttttgatga ttacttttcc gatcgaacct gtttcatttt tagcaaaaag gtcccaaccg
    53701 tcatctgcat tatgatacgc gatacaaccg tcaaatacat ttccttctcc tattgtcagt
    53761 ttggccgcaa atccatcggc atcttcaaat cctggatcgg cattttcaaa agatgtacag
    53821 ttcttgacta agttatgcgc gggccatgct tcgaaaggat catttgagct accgctgatt
    53881 tggattcctg tatttccatt ccgataagca tgaatctctt cgatcaaatt atgatttcca
    53941 gaaatctgta atcctttttg catagctgca ctatttgtaa tgtcaaaccc tcgtaaaccc
    54001 cagtagtgac tgaacagtgt catcccattt ccagttcctt taaaatcaag tacaggacgc
    54061 ttgttttctg gatttttctc tgcaatcaac tgaatcgggt tttctcttgt cccgttaacg
    54121 ccttttggaa tcgtcaatcc tctttcgaag gaataagttc caggcgccat gacgattgtc
    54181 tgcccagcat atgcgtatct caatgcttgt gtcagagata atggattttg tctgcttccg
    54241 tttcctgcat ctgacccttc tggtgttaca taaactgtcg aatatttagg tttacggtag
    54301 tccactgtat gaagaatctc ttttgtttca taactagtca tctctacgta ttcttctggc
    54361 ggatagtttt tatctggcgt aaaactgaaa gtaaattcgt tcgttccttc catcagtttt
    54421 gtctgataat caaattcccg attcgcagtg atagccaagt tttttgcgtt cttaagcacc
    54481 tctcctgttg ctttattttt aattgtcaat aatccgtcag catttgctct aaaggacata
    54541 gtatgatctt ggtttcctga atgattacta gaggtcacag tgtaaatcgg atcaatcaag
    54601 cgagtaggtc tttcttgccc aggaggatct gtcgcaggat ctgagtaagc aacttcgatt
    54661 ccttctacct tgatcgtcat attccttgct gcagcaaatc ctgcataaat aaccgattca
    54721 tctgaagcgt ataatttttc ccagtcatac atgatgtcag aagccatttc ttctcctgtt
    54781 tcaggatcgt aataatatgc ctctaaacca gtattcgttt ttttcatctc tacgtccagt
    54841 ttagtaatcg agttcgtggc cggaaacaga ttcccatgac cgtttccgaa tatattataa
    54901 gaacctgttt ctttttccaa gaaccctgct gaagtctcta aaggtgttgt ttccactgag
    54961 acactcccag gagctggcgc agcttgcgga tcttcagatg atattcctgt gattgcccgt
    55021 gtccccaatc caagtcgcat cgtatatttg ctgccgctcg tgttgaccac ttcttgtcta
    55081 tccgagtccc agacatattc gatccgactg cctagtatgg caaatgaatt tgaataaaat
    55141 gcctgcccaa acaaatgatt ttttggtact gcgtctctta ccattaaagc aaatccttct
    55201 tgggcattcg tataggtcca agattctatt tcgatcgtcg cccgcatcct aaagttgatg
    55261 tctttgggga ttgcttgata ataataggtc attccatctg atccgcttga ctggaatttt
    55321 ccgccgttat tcgtggaaga gagccgcacg ccgtcttcta atggttcaat ggcataccta
    55381 ctagcacttg tgctttctcc gaaaacaacc cctttccact cttcatttgc ttcttcagat
    55441 tgcttttttg tcttttcatt ttctgattct ttcatcatag gatcatttcc ttcttccgta
    55501 tccatatctt cactagttct tggtatttcc tccattgttg acatcccttc aatgttcacg
    55561 tgtgcatttt cttcgttcac agactgttgt tcgttagcag caacttcaac gacagaaatt
    55621 aaaaccgggg tactgagcat aaggagattc atcattagcg cactgtattt tatacgtttt
    55681 tttaacaagt gtattcctcc cgtaacaaat tagaaagcgt ttacttaaag tattacctaa
    55741 tttattttta ctgtcaataa aaaacaaaat tataataagt gtattttata aaaagaaaaa
    55801 atataactta agttgttgat aataaagaga tttattaacg gaaaaagaat aagttttttt
    55861 aaaataacgt ataaaaaaga gcataaaaca aagtcgtttg ttttatgctc ttcttttatt
    55921 attttgtgct ttttatttta tcaaatcact ttgttttttt ataaaatttc tgcgcctagt
    55981 gtggactcaa aatgtctcaa tgcccattca tgtccaacgg gatcgaaact tgccacggaa
    56041 tctttgaaaa tatggagttt gtatcctaga ttatacgcat cgactgctgt atgcaacaca
    56101 cagatatctg tacatacgcc ggtcaaataa atatctgtga tctggcgttc tcgcaaacgg
    56161 atgtctagat ctgttccact aaaagcggaa taatgccgtt tgtctatcca ataaacattt
    56221 tcctcttgct catagtcttg gtataaactt tttaaagagc cgtacaattc ccgtccaccc
    56281 gttcctatca cattatgcgg aggaaataag cggttctccg gatgaaagga atcttttgga
    56341 tcatgcgcat caatggcaaa aacgacaaaa tccttttgat caataaactt ctttgtatga
    56401 gcagtcagct ttttttcaat tacttgtcct gctgtcccag tagtcagctt tccatcctct
    56461 gcaacaaaat catatgtgta gtcaatcgag atcaatgcgt tcatcttagt cctccttata
    56521 cgagatgttt gacaaattcg atcaccattt ctgctgatcg tttgcctgcg tcttcgataa
    56581 attcgtcaaa gctttgtgtc gccgagtgat ctgctgtgtc gctcattgca cgtacgatca
    56641 aaaatgggat attgaactga cgagctgttt gtccaacagc ggctccttcc atttcgcaag
    56701 ctaatgcttc agggaaattc gttaagattt ctttgatttt atctggcgag tcgacaaatg
    56761 tatcacctgt aacgatcaat ccttcttttg cgttcaaatt cgttgctttt gctgctttaa
    56821 ccatttcact gcgcaaatat gtgcttgctt cataatatag aggcattcct ggtaattgtc
    56881 ctgggttgta gccaaatcct gttgcatctg catcaaaata agctactttg tctgatataa
    56941 cgatgtcacc tacttgtaag ccttcaccga ttccgccagc tgaacctgta ttgatcacca
    57001 tgttcacgcc gtattgttgg atcaacagac ttgtggtaat agatgctaag acttttccta
    57061 taccggaacg tacgacgatc acttcgtggt tacctaatga acctgaaata aataaagcgc
    57121 ctgcgcgttc ccatgataat ggttcgctta ggttctctct caaaattttt acttcttctt
    57181 ccattgcgcc gataataccg attttcatct ttttaattcc ttctttctat ataaatgcga
    57241 ttgctaaaaa aaccacacat aacattagga taacaataat aagagatttc atcaaaaaag
    57301 agttccattt ggtcgtcttt tcccgttcgc tgatccttgt tttcgtgatt ggtttattct
    57361 ttttgctttc tttacgataa aagctggcaa tctttttttc ttcttgctga taagctgttt
    57421 ctgcttttcg ctgttttttt aatgactcgc ttgcttgtgc ttgttgtcgc ttgcgaagtt
    57481 cgcttcgcgt aataagcggt cctttggcca taagccgatc cctctacttt cctttggtca
    57541 atagttccca gtattgaatc gcataaatcg tctttgcatc acaaatcaac tgatctttca
    57601 tcgcttgttg cgcctcttca agagttagat gatacaactc cagtacttcg tcttcatctt
    57661 gtgctaaagg attctcgacc ttctctaccc cttgtgcatg ataaatatga aggacttcat
    57721 ttgcaaaacc tggtgaaaga tacatcgaag taagatggct cagcgacttc gcacgatagc
    57781 cagtttcttc ttctaattct ctagctgctg tcatttcggg attttgtcct tctcctggat
    57841 cgatttttcc agctggaatc tccaaaatca ctttttctaa aggttttcga aattgtttga
    57901 caagaagcag acgatcttgt tcgtcaaaag caatgatccc gacaccgcca ggatgaaata
    57961 ccagttctct cttcgaagtt cctccatctg gcaaacgaac ttcgtctaca gcaacatcaa
    58021 taatttttcc gctatagatt tctttgcgac tgattgtttt ttcttcaaat tgctcattgt
    58081 tcagcatatc ttctcctgct ttctttgtaa tcataactgc ttttttttca tttttcaatt
    58141 tttctcaaaa actccttatt agtttaccat agataaacct tagattttct ctaaatatag
    58201 aaaatccttc tttagtccga tactctttct agtagtccgt atgctaaaat aagtatgtaa
    58261 tcgaataacg aaaagaattc aatactcaac gataaatgac atggagggtt tattatgata
    58321 aaacgaaatt ggccgtggct tctagcattg gtcttgctat ttctctttgt ccgaaacaat
    58381 agtttttctt ttgttctagg acttttattg ataggtgtag ttttagtttt gtggggactc
    58441 tttggttcgc gcaggaaaaa gggaaaacaa gaagaaattc ctgcactgac tgacgaactt
    58501 gaaactcact atgaaaaaag cggtatgaca gcgaatgaaa tttcattttt tcgacaaaca
    58561 atgaatcaaa caaaaaatga gatcaatcaa ttgcaaatga atatgcagca gacagccaaa
    58621 cttaaagcaa tcgatctccg ccatgatcca gtcaaagcag cgaaagcttt attcaaagaa
    58681 ttggtgaaag aaccaaaccg actgcatgaa gcaagtcagt ttctttatac gcatctgccg
    58741 aatctagttg atttgacaaa taagtataat gagatcaatg accatgagat caaaaacaaa
    58801 cagacctatg aaaaactaga agaaagcgcg cagatcatcg accagctgtc agctttaatc
    58861 gcacaagatt atcaaaaatt cgttgctgag gatctagatg atatcgacgt agagatatct
    58921 gttgcgaaac agagcttaaa gcgtgataat aaatatgaac cagaacaaaa ggattaactc
    58981 ttttgttcat aaaagaagac tcggaacatt cagatgatgt ctggctgttt cgagtacttt
    59041 ttctaaagta gagacattca tgtaagaatc gagaacagat tcaacaagga ggaatttttt
    59101 atggatcata aaccgactga aacaaaagat gttacaccag ttaacgatac acttgaagat
    59161 ttattgaaca acccgttttc aactccaaca gatacgctga ccacttcaca acaagcggag
    59221 attgatcagc tccaagagca acagacagct gctcgtttaa ttgacaaact accggcggaa
    59281 cgccaagaac aagctaaaca gctagcagca aaaatcgatg ctaatgatgc acagtctgtc
    59341 atcagctacg gatcggctgc ccaagcaaaa ttaagtgaat tctcacaatc gatgttgaat
    59401 catgtgcaag cacaagatat tggtcctgta ggagattcct taactgaact gatgtatcgt
    59461 ctccaagaag ccaatcctga cgaactgcgt gccggagaag ggaacatctt ccagcgtgtc
    59521 ttcgggaagg tcaaacaatc gatctatgaa gtgacagcaa aatatcaaaa gattggtgca
    59581 caaattgaca agatttcagt gaaattagat aaagaaaaag acggtctgct aaaagataat
    59641 ctgatgttag aacagcttta ccagaaaaac aaagactact ttgatgcact gaatatttat
    59701 atcgctgctg gagaattgaa aatggaagaa cttcaaacaa cgatcattcc agaagcgatg
    59761 aaaagagcag aagaaactgg cgatcagatg gatgttcaga ttgccaatga ttacacgcaa
    59821 ttcctagatc gtctggataa acgtacgcat gatcttcgct tagctagaca gatcacgatc
    59881 cagcaagcac cacaaatccg tttgatccaa aacacaaatc aggccttggc tgaaaagatc
    59941 caagcatcta tcgctacagc cattccgctg tggaaaaacc aagtcgtgat cgcattgacc
    60001 ttgcttcgtc aaaaagatgc agtaactgct caacgccaag tctctgaaac aacaaacgac
    60061 ttattgaaga aaaattcaga aatgctgaaa atctcagcca tcgaaacagc gaaagaaaat
    60121 gaacgtggaa tcgtggatat cgagaccctt caaaccactc aaaatgattt gatcgaaaca
    60181 atccaagaga cattgcgtat ccaaaaagaa ggaaaagaaa aacgtcgtca tgcagaagta
    60241 gagcttggac acatggaaga agatctgaaa caaaaactgt tggatttaac ccaatagatc
    60301 acctttcgct tgttactaaa acaaacgccc aacaacagtt tattacaact gttgatgggc
    60361 gtttttctta tctttcttct aacttctcca ttggagtcta tttctcattc atcttgctgt
    60421 gtccatagcc atagccaaaa taaatgatca gtccaataac cattacgata ccgaacacga
    60481 tccatgtcac agtttgcaga cgaagcatca atacaataca agtcaagatg gagacgattg
    60541 gcaatagcgg aacgaatggc actttgaatt ctccagcttt tggtttgcca aacatttttc
    60601 tcaacttcag taagcctaga ctaacaagca tcaaataggc gagcgccatg atattcgtta
    60661 gttccgctaa tacattcaat gggaaaaatc cggcaaagaa tacagctgcc acaccagcgg
    60721 cataagtcgc ttttttcggt gtacgatgct tttgatcgat ttctgtcata aacttaggaa
    60781 gtaagccatc cttgctgaca gcaaacagta aacgagcaag tgaatacatc atcgagatcg
    60841 ttaccgtcag tagcgttaaa attgccccta cagcgatcac cgcgcccact gttccatgtc
    60901 ctacatacct cattgcaaaa gccactgggt cattgactcc tagttcagta tacggaacga
    60961 ttcctgtcaa gatcagcgtc acgatgatgt aaaggacagt agcaatcagt atcgaaccaa
    61021 tgattcctct tggcacatct ttttgcgggt ttttgacttc ctcggctgcc attgcaacgg
    61081 catcaaatcc caagaaagca aaaaacacaa gagctgcacc atctagcaca ccggaaaaac
    61141 caaaaggcat gaatggctgc cagttatcag gtttaacgaa aaatattcct acaatgataa
    61201 acaacgcgat gataccaaat ttgacatata ccatggcatt attcaaacgt aatgctttct
    61261 tggcttcaag cgacacgatg taagtgacga atatcactac gagcaccgca atcaagtcaa
    61321 tgtatgtacc attttccgga ttgtacccgc cagttaatgc ttgtggaagt tcggccccta
    61381 aacttttcaa aaaaccttgc atatagccag accatccgga agcaacagag gaaaccgcta
    61441 gtaaaaattc cccgatcaac aaccaccctg tcagccaggc agcaaattca ccaaatacaa
    61501 catacagata ggcatatggc ccaccaatca ctggcactcg cgaagcaaat tctgcgaaac
    61561 ttagtccaga caaaatgaca acgattgcag ctaagacaaa tgaaagtgac agtgctggac
    61621 cagcattttg attggccgca acccctgtca ccacaaatat tcccgtaccg atgatcgcac
    61681 caattccaag catgatcaaa tcagacgttt ttagttcttt tttcatctca ctcgtatgat
    61741 tgagattgat ctctttctta cgaaacaaat ccattgaagt tcctccccat tttaggttgg
    61801 aaaacagcga ttaaactgtt ttcatatacc ctaaaaggat accacctaaa atgactaaaa
    61861 ggcaaccaac aataacataa atgagctctt ttttcgtttt tctttcgcca aggatgaaaa
    61921 tcccacctaa agtagagata atgatcccca tttgtgaaag tgagaagcca acagctaaac
    61981 caagtgattt gactgttaga agcatgcaga tgtttcctaa gccccataat aatccacaga
    62041 tcatgttttt ccacacaaac tgatcgactt taactttacg gaaagcaaag aagcttgcgc
    62101 ctaagatcat tccgatactt tgaggtaaga taattgacat cgcatccaaa ttcgcccagt
    62161 tgacgatgat ggtatataca ccataaccaa tcgtagagaa gatcaatgct ctaaaaccct
    62221 taccaaaatc aagcatttta ttatctgtat ctatttttcc ttcgtcatct tgtcgggcag
    62281 tcaaatacgc accgcttaca agaagagcta aagcaataat accgtataca taatctttcg
    62341 tttgtgtcca ttcatggaag aaaacggctc ccgcaatcgt attcaaaatt aattgaaaac
    62401 cagtagacaa tggtagccct acagaaacac ccatgtactt cattccatgg aactgtccat
    62461 tttgtccaac actccaagct aagccggata aaaatccgat caagaaaatc caaggggtga
    62521 ttacaggact aacaacgaaa aagacaacta aagaaaacag aaatgcaccg atcgtcattc
    62581 ctagtgtttg ttgattggca tcgccaccaa ttttaccact tactaaacca atactacccc
    62641 atgccaacat agggactaat gctattaaaa tttccataac caatctcctt ttatttcaca
    62701 tgataagaaa cttatcagcg aaaacgttaa caacacaagc actttttttc gtttctaacg
    62761 attttctaac gtttttcatt ataaaaattt aagattggga aaaataaagt cattgttgtc
    62821 ccttttcctt cagttgattc agccgtcaaa tggataccta atttttcggc cagatttttt
    62881 gctaaataaa gtcctaatcc tgttgaatgt gttttgctaa gtcgaccatt tctcccagta
    62941 aatcccttat caaagatccg tttcaagtct tccgctggga taccgatccc tgaatcttta
    63001 actgaaaggc tgactccttg cgcttccttt tctatgctca caatgatgtt tccgtgatcc
    63061 ggcgtatatt tgatggcatt actaagcaat tggttgaaga taaaagcgat ccatttagca
    63121 tcggtcaaaa cagtatgatc ctcaccaacg atctcgtagc gaatattttt ttggataaaa
    63181 taatttgcct gactgcgtat cacaggctga atgattcctt ttagtccata ttcttggatg
    63241 aggtagtccc gcgagaaact gtcgagtctt gcataataca aaacttgttc tacgtattca
    63301 tcgatttttg ataattcatt ttctagtaat gtatactttt gatcgtcaat atcgaactcg
    63361 atcgaacgaa ctagcaattt agaagcggaa agtggcactt tgatttcatg gacccatgag
    63421 tcaatataat ctttttggtc ttgttgatta ctgattgctg attgcattac ttcttggtgt
    63481 tcacggatca gctgattgat atattcttct accagttttt cttcttctgt cctcccacca
    63541 ctcaaataat tttgcaaagg ggaagttttt tgtatgtcgg ctaattttcc ccaccatctt
    63601 ctcttgctca agtaaccacc cagcagataa aaaatcagaa agacgccctc aatcaaagct
    63661 aaataagcaa ttgtgctcaa atcaactttg atgtttggcg ccagccacat cacaaagatc
    63721 gtcaatgcaa taaaaaacag ccatcccaag atcaatgtcc actgatcttt caaatattta
    63781 aaaaaattca tcttaaccgc tcatcctttc agggaacgat atatccctgt cctacttttg
    63841 tttcgatata tccttggata cctgcctgtt cgatttttcg gcgtaaccga ttgatgttga
    63901 ctgtcaatgt attgtcatct acaaatcgtt cgtcatccca cagtgctcgc agcaattttt
    63961 cccgacttaa gatctttcca ttgtgcttca tcaaaataaa taatagacga tattcatttt
    64021 tgctcaaatc aatgatctct ccgttgattt ccatacttcc gttgtcaaca ttcaacgtaa
    64081 tgccattgtg gctcatcatt tcactagata attctgtgta gttgtaagaa cggcgaagta
    64141 atgcattgat ttttgcaatc agtacatcga tttggaaatg ttttgtcaca aaatcatctg
    64201 cccccatgtt catagccatg atcatatcca tattcgtatt cctgctggag ataaaaatga
    64261 tcggtacttt cgatacttca cgtatttttt gacaccaata atagccatcc aaaacgggaa
    64321 gattaatatc tagtaataca agctgtggtt cttctctttc aaagtctgag aacacttgat
    64381 tgaaatcggt cgttccaaag gtttcaaatt gccatttctc aagttcctcc atgatcaatt
    64441 gtcgaatgat ctcttcgtct tcgactacca tgatttttgc cataaaaaat ccctccatct
    64501 ttcttcttgc tactacaata tcaaagaagc ccttgtgaaa caagttggct tacctggaaa
    64561 atgtcatttt agcaaacgtt aaaaaaaact ggaaatatgc gatttaatcg cgtttgtctc
    64621 ttccaagcag cttaaattta ttatgttatt cttaataatt ggtctattat ctgttatttt
    64681 tctttcgttg tttttgtctt ttaaattttc gataacacca ataaccgaaa agcgttagca
    64741 ctagcaatat acctagaagg agtataagta atcgataaag atggtatttt tttgtctgtt
    64801 ctatctttga agagacttct tctgtaaagg gaactcgtct tcctgtcact agtaatcggt
    64861 gagtgttcac ggtgtaaggt gtacaagtga taagtgtgac tagatcctgc ttttctctaa
    64921 ttgctaatgt atttgttttt gttggaagga caacaatttt ctcaacgact tcataagcga
    64981 gcatccgacc atacacttca agatagaatc ggtcatcttg ctccatttct tcaagatccg
    65041 taaagagttt tttctcagca ataccgctat gggcggtaat cacggaatgt gtactctctc
    65101 ctccagttgg aaaagaggtg ccttgcagga ctgtcgctcc tctatctaac aaaagatcat
    65161 tcgtttcatc aaataaaggc aagctaacat tgattttcgg aatatagatg gctccgatca
    65221 tatgttgttc gtaatattct tttcctggat tttggacatt tctaaccgct tgctcaaaag
    65281 gatctttgac ttgtcccatt cctgggatta ctgatgtatg agcaagcgtc tgatttttct
    65341 tttcatgcac tgctaattgt tgctcatgtt tttcttcttt atcgcgttca agctgctttt
    65401 gataattctc gatccttctt tgatccaaat aattgctgag agcatctgct aaaaatggat
    65461 atgtaaaaac tgcagctccg gcaagaaaca tcactgccat aaataattgt aataaaagac
    65521 tgatttgggt ttgaggtcta ttacctattt tctttttcat tttcttcacc ttcttattta
    65581 aaaaaactgc agcaaccttt ataaaaccga ttgctgcagc ttatttttgt tgcttatact
    65641 tcagacttca ttttagattt acggtaccag ctgtacgctc caatcatcaa acttattccg
    65701 attagtaaga atgccaagag cccgttccca ccagtagcag gaagtgaccc gcgtttcata
    65761 ttcggtactc tttctggccc tggattttct actcctacga tctgaatttc ttcattatag
    65821 cttccataag agatgataaa ttcagtaaag gctgcctcac ctgttggaag tacataacct
    65881 tctggcgcca tggtctcttt catttgatag gttccataat ctaacccttg aacctcaaag
    65941 tatccatttc catctgcgac aaattctgtt gcttgtccgc ttgttgccca ctcaattcta
    66001 tctgctcctg attcataaac agctaaaggc gactcccctt tataaaatat tgcgtattca
    66061 gaacggtctt cattccacaa ttcgaatctt gcatctttaa gcaattcact gcttgatgca
    66121 tcatattttt caaactttct tccacctgtt gtgactttct ctggcggctc tactccttct
    66181 tcataatcaa attcatcatt tcgtccgatt tcaataaccg ctgtgttgtt atggaaatca
    66241 tccgcttcta gttctgtgga aaccttcaaa ttatacgtga tcgttaaatc tcctccacgt
    66301 gcatctgcta atctacctaa agtatctact ttggctactc cattttcttc tgtcaaaatc
    66361 gtcacgacaa acccgttgct tactacttct actatataat cttgtccttt tgttaaaatc
    66421 gtctcgccgg ctcgcacttc tatttcctga tcataataag ttagaccaac tgtaggcgta
    66481 tctgtgattt tgtaattttt gataacagcg cttccgtttt caaggacaga atcgatgaaa
    66541 taaggaatag gtgctgtgat cgtgtaagaa gccgtttttc ctctttctag atcaatataa
    66601 gaaatcgttt cttgttctcc agcttcatta ataatcgtta cttgtctaag gtcgctttcc
    66661 tctacattca tctgtttatc tgcttctctg atttcgtttt tcggatacaa atgcacatct
    66721 gtattgtatg tgtctttcca tgtagaacca tcccacacgc catccaccat atcaggcatc
    66781 atcactggca tcataacaac cattggagac gcgattctgg aaatatgagc tggcgaataa
    66841 gtttctaaga aaagatagac tttatctcta gaagaaggtg cttcttccgt cacttggacg
    66901 gtagagaata ccgcttctcc agcttcattc gttgtttgtt tgtcaatact tgtcagtcga
    66961 tattttaaaa accaactttc tgtgtgccgt ttttgaatcc attcaatcgc ttcggcagaa
    67021 ttaagaccgt cttctcgttc cggtgttaaa ggattatctc gaatcaaatc gtaatatact
    67081 tccgttacgt caaacaaatc aaaatcgact cctggaatat tttcttcacc aaaatccgta
    67141 gaaaccaatc cgctattcgg tcgttcttcc ggaacagatg agaaaccttt tttgtgtaat
    67201 gtaatcgtca cttcacttgg tttttgtgcg acttcttcgg ctttgactag ttttccgttc
    67261 ccgaaaatcc ctattcccgc agtaacgagt aacagtacac tcattcccgt gacaatcatt
    67321 tttttattca tttttttctc cccgttcttt ttttattaat aaaacagcac ctaacaataa
    67381 aagaattcct agtaaagaaa tcaacgttct tgcttcattc gtttgcggga atacggacca
    67441 ttttcctgat tgacctggat tgcttggacc actcggctct ttcggtttgt ccgttgtttc
    67501 ttttacggtc tcgttataaa cacgtggtgt tgcggattca taggcagaga ctggaacttt
    67561 cccggattct aattcagcca tctcctgtcc gttaactact acatattgtg gttggccttt
    67621 tgcatccacc caaaattcag ggatgatgac ctcgattctc ttacttgctt catccatctc
    67681 gtaaccttcc acacctttta attcttcaaa ataataggtg ccagaaggca agaaacgttg
    67741 tcctgtcgta acaaggccct ctttatccga aatgaatttg cttacatttt tgtcattcaa
    67801 aggatcattg tctgctggtt tgatccattg atttttcaag tcattagcag gagccatatc
    67861 taaataatat ttcccccctt ctatcatctg atatagagca aaaacagcac cttctaatgg
    67921 ttttcctttt tcagttgttc ctctttgacg tccatacttg aagaagtatg gatctcttag
    67981 ataaccgaca tttttaggat agatatgaat ctcttgcagt tcctcggttg attctgtcgg
    68041 gtgataaatt ggcaaaatag cagcaattgg gatagctgtc tgatcgatat caacatccaa
    68101 tccaacttct ggatcaatcg attcttcgaa aatcaaataa actgcatccc gatcttggtt
    68161 tttcttaggt gctttgatac gtgcaatccc gtctcctaat ccgccaaccg tatctttccc
    68221 agttgttacc gaagtcccta tttggacaag cctttcttct gtgattagct gacggatatt
    68281 gtttctgttc gtttcagcga tttgctggac gaattcttta ggagtcatgt tttcagcagc
    68341 ttttttttca tgataataat cggtcatgtc gtaaatggca aatgtcgctc catttagtgg
    68401 caccgtctgt gaaatgatat ctgctgtttc ccctgcttcc tcagagatag ccaagccatc
    68461 attttcatgg tattcaaatt gatcgttata atcgatatct cgaatcatcc gtttatgcaa
    68521 cacgaattcc acgacatctt cccctgattc ttctgccgac atcgcatgtg gaagaaggac
    68581 tgctgcaagc ggtagtaaaa caagtaattg aagcatcaaa cacaacagct ttttcattac
    68641 cttgctccct cctttttaga ttgtctgaca aacaaaccat aggctgctcc tgcaagcgcc
    68701 atgaagaatg cagcaagcag gatatagaat aatcgtccgg atcctcctgt agcaggcaac
    68761 ggattttcag gatcgttccc gacactaagc tgtaatctga ttcgatttgt ctccaaaagt
    68821 tcatattcta actcttgctg tagatcaggc gtctctgctt cttcgtttgg ataatacgaa
    68881 atctcaactt ttagtgtacc cgcttctttt cctggaacta cccgatcatt ttctatgtca
    68941 ggttcttctc gataactaat ttctaaaata aagtaacctg gaagttttct atagctgtca
    69001 ggtgacttca cttccttgat ccgatagacc ccttccaaca agtctgaagc attgaatagg
    69061 gaagaatcgt ttggatctgg tgccaagcct ccctcgatga tttcatactc tcctgcacta
    69121 ttttgtttct caattgaaaa ttctgcgcct tcaagacgtc ggctattcat actgtcaatt
    69181 ttcaaaagtt ccaaatcgat tggcgctagt tcatcttcta aggttacttg tagtacacca
    69241 tcgatgattt catagccttc cggcaaggga gcagtttcgt cttcgatgac cgtatcttca
    69301 atgataaact taccttcttc attcagtgca aactggaaca cttgatcaga tagccgatac
    69361 ccatttggtg cgacagattc cgagacctca tattcaccaa tagacagatg cagaggttgg
    69421 tcgtaattta aatcgacata cttttctact cctacggcac gagaatagaa gtatccgatc
    69481 ccaggcaaac gtttcgattc ttctggttct aacggattac cagtgtatgc tcgatacagc
    69541 gggaaggaag aatcccccac tttcctgata tcgaaacctg caccatctaa tgtatggcct
    69601 tcatggtcta attttctaac tcggatctcc cgataatcaa acgtattgta agcagtgaat
    69661 gctacccata aacgatcctc ttcatctgtt gtatacgtga gatattgcca attttcttca
    69721 cctgtttgct tgagttggat agtccctata gcattatcgt aaagtcgcaa gatccatgtg
    69781 tcattttcat caggttcttt gaaaccttct gggttctggc tctcaatgat tttgtattct
    69841 gcatagttgg tagtttcttc actatcctta taaacaaatg aatctggctc tccgattgga
    69901 tgaataactt tctcaccatc cacgattaca tatttcccgt cttcatctac tgaatagaac
    69961 gtgagtctcc ctttctcttc ctctacttcg ttcacaccag aaagcccttt cgctactgga
    70021 tatccgattt ccagtccgtt accatctgtt tcataaagtt caaactctgc gccattgatt
    70081 ggcatattac tgccttcaat tagtttattg atattcatgt ggattggctt gaattcattt
    70141 cgcatttcca gtttgacata caactcatca tactctgtgc cttctatgtt aggctcttgt
    70201 acaacagaaa gcttgtgata ttctccttca taataggaat tcggatcttt accttccggt
    70261 ccgccgactc tcgaaccaga tgtatattca tctacaagct cgtagtgacc taacatctca
    70321 tcttcgttgg ctgcatattt tttgatttgg aattgatacg tctcatctac taactgataa
    70381 cgtggtggtc cagcgatctc tctgatccaa aatatgtcgc tgactgctcc agtttcatct
    70441 cctaaaatcg gcatcttcgt ttcaaagatc gctattccgt ccgtggtagt tttacttgtt
    70501 tcgattggtt cggcttcccc actctctgga ttttggtcat ataatgcaaa ttcgactcca
    70561 gccaacggct cgccattttg acccttcttg ctgacttgga aattacggcc tttatgttcg
    70621 taacgaatgg aagaagatgg gaaatacatc cgtccagtag tgatatcatt ctcataatcc
    70681 tccaggttct gaatataggc catattgttc actgtgcaaa aatttccgtc ttgagctccc
    70741 gcatcaagtt ttacttggta cttgatcctg accatttccc cttcacctaa agaaatcgga
    70801 gaaaatttat aagcttggtt ttcccgtccg acatacgttg tcggagtcgc cactacttct
    70861 ggtggggctt gtccatgacg ttcttcatcc catatttctg gctcttggta cttcatgccg
    70921 ttttctcctg gatctagatg gtaaaactga atatctgccg atctgccagc tccaaatggt
    70981 gagacattaa aaccagtttc atcataaagt gtggcttgct ggatcgttct cttgaaggta
    71041 gaagagatat cctccaaggc tgtaacaatg tcattcttgt tgttcgcatt gtaatatcgc
    71101 ggtgttacgc catcttcttc taaatcagca atatttttca atacatttcg tccaatagca
    71161 tctagtcgtt gtctcccgct aacgtttcca tcgattccta aaccaatgga gaagatactt
    71221 ggcgggttct cttgatactt atttttcagc caatgatgat aagcaactgt gttgatcgca
    71281 gatgactttt cttgcgaagt aggactgcca ttcgtattgt tattagaaga accatcacca
    71341 acccagtgag tcgctttatt atctccttct tttacctctc cataacgata gtgtacattt
    71401 acttcatctt ctggacggtt aaagtcagat gcataggtaa ctggatgagt aaaccctgca
    71461 ttagaagtat tcccagaaaa ctcctcaaaa tttctgaaca aaacgtcatt atcttcagca
    71521 atcttatctt ccatcacagt ccagttgctc caagctccat tagtgctcgt acgataactt
    71581 gactgaatcg gtgcataact gaaggtcggt gtcccatctc ccacaacgat taggattttt
    71641 tctggatttc tattttctcc gccattgtct ttatacaatg tttcatagcc atttttaagc
    71701 cctagtgtaa gcggagttcc tccgattggt gtacgattta ttgctgtatt ctcacttccc
    71761 gtcaaacgat tgatatcatt tgtcaatgga aagatgtccg aactgatttg ctcatggtta
    71821 ttgatagctg tactattatg gtaaaaattc accataccga ttcgaatatc cagactcggg
    71881 tcagagagca gatctttact catttcgttt actgcctcaa tcaacgcagc atttttagtt
    71941 tgactattgg tcccagcggt taattcactc atactcgctg acttatctag aacaaaaacg
    72001 atatcgattg gactgatttc ctttttctcg cccccgatga catctagata gacattgtaa
    72061 agggttgttg catcttcagg atgtagatca gatgctgtgc tgcttggcag aacaggttct
    72121 acccattttt tgatgtacgc cccgttataa tccaagtaac tgttttcaaa attccgttca
    72181 tttccccaaa gctgaacggt ttgagctgga acgtttctat cttcttcgcc tgcagttgat
    72241 ccattattag tagcatagtc gtaatttcgc cagttcgctc ccccgtttgt ataatcacta
    72301 tcatcgatca agttctctcc gtcatatggc gagcctttgt ctccttgata gcgttcattg
    72361 ctttgtgtcc aatttcctaa cacttttgta tagtcattcg ttgtaaactt cgggtagcga
    72421 tgctgttcgt tttcaacttc tccgctagga tagatatatt cataggggtc ttgcgtagct
    72481 gttggcccaa gacgctccaa aataccgttg aataactggc gttgaatgcg ctggagtgct
    72541 tgaccgctgg gtacgattgg ttcgtccagc ttttccatct cattttcttc gctacgttga
    72601 atttcagcgg aatcttctgt atcagtttca ttttcttgta attcttggtt atctattctt
    72661 tcatcaacca ccacttgaag aggtagttac ccttcgttca catcggggat cttttctgtt
    72721 tcaaaaatca atgtgccggt ttctgcttct tttgaataaa cttttccgac ataccattct
    72781 tgaccatcga tcgtctcgac ttttaggtct tctccatctt catcatagga ggatagacct
    72841 gttccacgaa cgttttttac ggtaccaatc ccggcggctg ccgcatcgat tgctaaacgt
    72901 attctacctt cattggcact ttcctttttt tcaaaagcca gatgccattc aagggtttct
    72961 tctttttcaa tatacttccc ttcaattttt gcatagtcct catctaagaa tacctcatcc
    73021 tcctttgttt ctgttgactc agcagctaca tccagggtcg tcatatgata gactgactgg
    73081 aaatatggtc caaatagcaa actgacagtt actaactgca aaagcttcaa ttttaaccct
    73141 ttcacatcct tatactcctt tcaaaaatat ttgtcttctt tcacaacaat caacaaaaca
    73201 atgcccatct aattataatt taatttaatt ttaattttaa atcaaatgat atccttttta
    73261 gttaaaaaac accaattata caacatttat tattaaaatt aaattataaa attttataac
    73321 gttctcattt aacaaaactt aagttattaa gacaaatgaa taaacaacta cgctttcctc
    73381 gaaacaaaaa aataaagcac aaatgcgcat ttacgcactc atgctttatt cgtacaatta
    73441 attcagataa cggcagcgca taggatgcca ataacacttc ctaaaatagc tcctcctaaa
    73501 atgtcagtag gataatgtaa aagtaaaaaa atcctcgaat aagcgatcaa acaagcaaat
    73561 aaaaaggcta atattcctgc aactggactt aattcgaaca acgcagctac tgataaaaag
    73621 gctagcgctg tatgtcctga aggaaaagaa gaagtcttcg gtttacttgt gtaaaggaca
    73681 agccgacgat ttttttcata aggtcttttc cgtcttgtcg tttttttgat aaaaagcaaa
    73741 ccaacaaaaa aatagatcaa actgccgata agaatggcta gaccatattc tctagtatca
    73801 ggtatcaaga ctaaaacagc agcaaggagc aaccaaataa aaccaccatt agagatatta
    73861 ctgataaatt tcaaaaaatt gatttttcga ctatccgttt tccgttgctt ggcgaacttg
    73921 ttgataatac tatcatcgat tttagcaatc aaacgcaaca tgttattcct cctttttaag
    73981 aaccttttct tgcgtacgat ggataaaaag caggacttga aaataaaaca agaaactgaa
    74041 acctaataaa gcaaaataga ttggtcctgc aaaccagaat gcgacaagaa ggatcaacgt
    74101 gcctgccaaa tagaaaagcg cgcttttgaa atcattaaaa aacaagaaaa atgcagtaat
    74161 ccaagaagct tctttttgtt ccagttgaag caagattttg taactaaaga gaagcgcgct
    74221 taatagaagc aaaaacaacg tagtatacat caatgtatag ccaattcccc caccccaata
    74281 gcggaaaatc tgataatcga tcaatagaag aagaaaataa acacttgcta aactatcttg
    74341 ctttccataa agagagaagt tctcacgaaa ctttttccag aatagtgaac cgaacttttt
    74401 ctcttcattt tttttgtaat gataaatcat atacagaact gttcgacttg cagttcctaa
    74461 tgtgaaaaca ggcaagacgc acaggaacca tagtagctgt aaatggataa gacgtggaat
    74521 ccattcaatg atttctaaga aataatcatt ggctttttta ccaatcgatc gattcatcat
    74581 ccttttgaac ctcctgtcaa atccatccct tcaatgaaat aacgttgtgc aaaaaagaac
    74641 agcaaaatcg tcgggatcaa gacaagtgtt gctcctgcca tcagataatt ccattgtgtc
    74701 gttgcttggt tctgaaaaac ttgcaagcca atctgtaacg tgtatttttc ttgatcttgg
    74761 atatataaca gaggacctaa gaaatcattc caagctgcgt tgaaggaatt gatcgcaatg
    74821 gtaatcaagg caggtttcgt caatggaagc atcaaatgac tccaaatata caaatgattc
    74881 gctccgtcga tttcagcagc ttcgatcaat tcattattga tcgataagta aaattgacgc
    74941 atcagaaata tattgaacgc attgccgaag aacgatggaa cgatcagggg caaataagta
    75001 cctacccagc cgatcttgga gaataataca tattgaggga tcatcgttac aaaccccggg
    75061 atcatcatcg tagaaagaac caaagcaaac atcagctttt tcccaggaaa atcaagcttc
    75121 gcaaatccat aagcaataaa agaatttgaa aggacattac cgatcactac tagcacagtg
    75181 atgaataatg tatttcttgc atagcgccaa aaaggaaagg cggcaatcgt atccagatag
    75241 ttttcccata cgattttttc tgggaaaaag gtcggaggat aacgaacgat ttcttccatt
    75301 ggtttcaatg aagtaaacac catccaaagc aatggaatga tcaaaatgat tcctattact
    75361 gtcatgagga cataacttag tatttttttt gtttgccgat tcacactggt ctttttgaaa
    75421 taatccatca tttgccgtca cctccagaat aatagaccca cttaggtgca agcttcaaat
    75481 tgacaaacgt cagaaccaac acgatcagaa acaggatcat tgacatagcc atcgcataac
    75541 ccatatcaaa agctacgaac gccttgttcc acatatgcag attataaaaa agcaaagaat
    75601 ttgcaggtcc gcctgttcca tttttcgtca tgacataagc ttcttgaaaa atttggaaag
    75661 aaccgatcgt agaggtgatc acatcgtaaa aaataatagg agtgattaat ggcaaagtaa
    75721 tacttttgaa ctttccccaa acgcctgctc catcgatttc tgctgattca tataaagatc
    75781 taggaacatt ttgtaatgta gctaagtaaa gtagcattgc tcctccagaa ctccatattt
    75841 tcatcaaaat caacgcaggt ttcgtccatt gtggatcaaa cagccaagct ggtccaggga
    75901 tatggaacca gcccagaaat gtgttgatca atcctgcaga tggagaaagc agctgcatcc
    75961 ataagatgta gactgctact cctgataaga ctgccggtaa atagaaagct gtacggaata
    76021 cactgattcc tttgattttt tgattgagta ctgtggctaa aaacacactg aaaatcgtcg
    76081 tcagcggtac attgaagatg acgaaataaa cggtgttgta taacgatttc caaaataaat
    76141 catcgtgaac gaacattctt ttgaaatttg ctagaccaat gaaattcatc ttggatgtca
    76201 aattataatc ggtgaaacta ccaataactg aaaaaagcat aggccctagc ataaatacca
    76261 agaacccgat aataaaaggc gagataaata aataggccca tcttgcttct tttttctttc
    76321 ggatagataa acgcttcaaa ttttcttgcc tcctctgtac ctagacagtc aaaaatggat
    76381 tattgatttt gcttgactaa gttttctact gctttttgtg catctgccaa tccttctttt
    76441 ggtgattttg ttcctaaaag aatctggtca atctgttcat tcactaatgt cgtgtaatcc
    76501 ggagcatcta aaggaactgg tgtgatcacc gtatttttaa agttttcgtc cgccatttga
    76561 tagatcattt ttcctttatc cgttaggttg tcattattca ctaaattctc attggctgtc
    76621 ttgctcgcca tgatatcaaa acttttttcg ccgaattttt cttgtacttc tggagaagaa
    76681 agatatttga tgaattcata agaggcctca ggatcttttg ctccgtatgg tacttccaag
    76741 acgaatccgc caccccacga ccaatggcct gaaccttctt ctttttctgg gagctgcatc
    76801 acgccaaaat caaaatcatc cggtgcattt tccatcaaac tagagtaata attgatattt
    76861 tgggcacgca tggctactaa tccggaaata aatggatcag atacaccaga cccaaattcg
    76921 gcttctaatt tattgatcgt atttttccca taataatctt gccagtcttg gatccactcc
    76981 aaggcttcca ctttattttt agtatcaatc ttgacttttt catctttgtc aaaccaacta
    77041 gtcccgttat ctgcgtttag cgcccataca tcagccccta aattccataa tggataaaaa
    77101 ccgatgcgtg taaattcttt tccttctttt ttatctagtt tacgtgctgt ttcttctaat
    77161 tcatcccaag ttgtcggtag atcttgttca ctgattccag cttctttaaa taacttttta
    77221 ttataaaaaa tgacttgtgt atctgtattg aatgggatcg cataaggatc accttcatat
    77281 tctactgtat cccaaagctg cggataaaaa tccttagaag tgtctttttc tacatatttg
    77341 gagagattag ttgcttgctg tgcttctgct ctttgggcaa cggaatttat gtcctgtaca
    77401 ataacatcgg gtgcatttcc agctgttatt gcagataaag atttagtcca gatgtcaccc
    77461 catggctgat acttataatt tacatggatc tcgttttgtg attgattgaa atcctcaatg
    77521 atttcttcga tagcttcttt tcgtgcaccg gatccccaaa aggaccagaa atcaatattt
    77581 tttcttccat cacttgtttt ttcagaagaa tttgctgcac ttgaactggc atcgcttgca
    77641 ttacttgatc cgcatcctcc taataaaaac aatgctgaac ttgccagcaa ccctgtaaac
    77701 aataattttt tcatatttta cctccaaatt ttaatgtaac cgatgccggt tttaatataa
    77761 cgatatcatt ttgttctgaa aataacaatt tataaggtat gattctaaaa aaattattaa
    77821 tgataccaat actttatata attcgcgagc gctttctttg aattttttaa tctttcgtag
    77881 cagaatgaga atgtattaaa aatagattag gaggaatttt gatgtttaga caccagaaag
    77941 aattgcagtt tgaagcaaaa ccagatcgtc cgaatccatt aatcgcgaaa tatctgcagg
    78001 agctgatcgg cggccaatat ggcgagatga gtgtagctat gcagtacctg ttccaaggct
    78061 ggagctgccg aggagaagaa aaatataaag atatgctgat ggacattgca acagaagaaa
    78121 tcggccacgt ggaaatgttg gcgacaatgg ttgctcgttt attagagggt gcgcctgttg
    78181 aagatcaaga agcagcgatg caaaaagatc caacaatcgg tgccattatc tctggaatga
    78241 atccacagca cgcgatcgta gctggcttag gacctcgtcc tgctgacagc gtgggcaatc
    78301 catggagcgg tgcctatatc attgcgagtg gcaacttgtt agcagacttt agagctaatt
    78361 taaatgccga atcacaagga agacttcaag tagcacgtat ttacgaaatg atcgatgatc
    78421 gtggcgtaaa agatatgctg tctgttctcc ttgcaagaga tagctatcat caaaatctat
    78481 gggaatgtgc agtgaaagaa ttagaagaaa aagaaggttc aatcttagtc ccaagtactt
    78541 tcccgcgtga caaagaacga aaagacattg cttatgactt ctataacttc tcagagggag
    78601 accaaagtag tgaaggttct tgggctagtg gcaaagcact ggatggcgaa ggcgaatatc
    78661 aatggttgaa agaacctaag atcgaaggcg ctgcaccaaa attagaacca gtaacaccaa
    78721 aaatgtatgg gacacctcca ttcaagaaat aaatatctac ccgtagaaac acccgttttt
    78781 tgttccacac gctttgtgag caaaactgac taaagtcatt aatcataacc acccttcaaa
    78841 agggtggttt tctctagagc tataagctca aatcaccggc cagcgcctaa agacgctggc
    78901 tttcatattt tatgttcaag ccatattgcc tttgccagtt ttgcccacct cttaaaaggt
    78961 ttatgtacta ctccttagcc cttaaaagga tcgtcttact ctcgtacact cagcttatct
    79021 aacgcactat catgtttttc ttgttcttga atgtactttt tgatggtcgc ttcattttgt
    79081 cctacttttg tcacatagta tccttccacc caaaagtgtc gatacccaaa tttatatttt
    79141 agatttgcac aatctcttga tgatctcctg aatattggct cgatattgat tataaattat
    79201 ttttcttcta tactttggtg tgaatacaat gtggtattta cacatctact ttgtacgggc
    79261 taaactattt ccctttattg ctccacccgc atagcaagtg gttttgtatt tcaatcgctc
    79321 ggcgcgctca attaggtcta aagacaataa atctaaaaac ccgctgcttc actgacagaa
    79381 acaacgggtt ttcagaaaaa aatgaaatga aaaagatctc ttacggcaat ctattttatt
    79441 tttttgattt tttataaatg gaaaatgctt caagaagtcc aagaaataaa gccgtataaa
    79501 aacaagaacc tatgaaatct ttatttatta gatattcaac agaaatcccg ataacactag
    79561 ccaacaatat atttatcaga agtgtaaaat aaggatgctt tttatagaat agaactagtg
    79621 ttgttttcat agactctcct ttcttcatat ttccaattaa tcacaaattc acggcattac
    79681 tagaaaaatt aaaggggcgc agaagtggaa aaatactctt cgactttccc tacaaaccag
    79741 gccatcttgt gtttaataac tataccaaat ctaaggtacc tctaaatcta aattccattt
    79801 gtggcggtga cctccaattt ttaactcttc tatctcccat ccagtataca taaccttgat
    79861 acttcgacca cttatatatc gaacacacaa atacctatcc attgcaggag tactacctaa
    79921 cttagttgct acttgcatct aaataacagc tgaaaagata ggacttactc ttagtaaagt
    79981 atctgaacta ttaattttta aactatcatt tttatcctca actacatgca catctgttgc
    80041 aacttcactt tctgtctcat ccagataaat gattaaggaa tcgggaattg caatatcttc
    80101 agctgacgct ggaatagttg agctaaatta gtcatcatat ctttattata aaataaccaa
    80161 cagcaaaaaa tacaatgaaa aacaaacttg gaactaatgt atcattaata aatgttgaaa
    80221 aattcagcaa ctcattactg tatgatttta gcagcccaac caacatatac cctataaaag
    80281 ctaataagat ggttaggaat cctttagaat ttttactcat tcatagacac ttccttaaat
    80341 aacttttatt atagaaggta ttgtaaataa taatattgac aaaaagtgac tcaaaataaa
    80401 aaaaacctta cattatactg gaatgaact
//
LOCUS       DACECY010000004        80225 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.004, whole genome shotgun sequence.
ACCESSION   DACECY010000004 DACECY010000000
VERSION     DACECY010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 80225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.004"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            173..445
                     /locus_tag="IWO38_000244"
     CDS             173..445
                     /locus_tag="IWO38_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312365.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            460..1383
                     /locus_tag="IWO38_000245"
     CDS             460..1383
                     /locus_tag="IWO38_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0312366.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(1523..2275)
                     /locus_tag="IWO38_000246"
     CDS             complement(1523..2275)
                     /locus_tag="IWO38_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0312367.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            2498..2860
                     /locus_tag="IWO38_000247"
     CDS             2498..2860
                     /locus_tag="IWO38_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ0312368.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            3497..4969
                     /locus_tag="IWO38_000248"
     CDS             3497..4969
                     /locus_tag="IWO38_000248"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ0312369.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            4981..5805
                     /gene="nadE"
                     /locus_tag="IWO38_000249"
     CDS             4981..5805
                     /gene="nadE"
                     /locus_tag="IWO38_000249"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ0312370.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(5878..6768)
                     /locus_tag="IWO38_000250"
     CDS             complement(5878..6768)
                     /locus_tag="IWO38_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ0312371.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            6966..8483
                     /locus_tag="IWO38_000251"
     CDS             6966..8483
                     /locus_tag="IWO38_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ0312372.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            8473..9744
                     /locus_tag="IWO38_000252"
     CDS             8473..9744
                     /locus_tag="IWO38_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0312373.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            9919..10815
                     /locus_tag="IWO38_000253"
     CDS             9919..10815
                     /locus_tag="IWO38_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0312374.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            10829..11692
                     /locus_tag="IWO38_000254"
     CDS             10829..11692
                     /locus_tag="IWO38_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0312375.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            11698..12261
                     /locus_tag="IWO38_000255"
     CDS             11698..12261
                     /locus_tag="IWO38_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ0312376.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            12271..13794
                     /locus_tag="IWO38_000256"
     CDS             12271..13794
                     /locus_tag="IWO38_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ0312377.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            13806..14765
                     /locus_tag="IWO38_000257"
     CDS             13806..14765
                     /locus_tag="IWO38_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ0312378.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            14851..15930
                     /locus_tag="IWO38_000258"
     CDS             14851..15930
                     /locus_tag="IWO38_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0312379.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(16182..17582)
                     /locus_tag="IWO38_000259"
     CDS             complement(16182..17582)
                     /locus_tag="IWO38_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ0312380.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            17800..19461
                     /locus_tag="IWO38_000260"
     CDS             17800..19461
                     /locus_tag="IWO38_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ0312381.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            19418..20116
                     /locus_tag="IWO38_000261"
     CDS             19418..20116
                     /locus_tag="IWO38_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ0312382.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            20132..21556
                     /gene="araA"
                     /locus_tag="IWO38_000262"
     CDS             20132..21556
                     /gene="araA"
                     /locus_tag="IWO38_000262"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ0312383.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            21863..23022
                     /locus_tag="IWO38_000263"
                     /pseudo
     CDS             21863..23022
                     /locus_tag="IWO38_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            23140..24999
                     /locus_tag="IWO38_000264"
     CDS             23140..24999
                     /locus_tag="IWO38_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0312384.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            25109..25543
                     /locus_tag="IWO38_000265"
     CDS             25109..25543
                     /locus_tag="IWO38_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312385.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(25982..27004)
                     /locus_tag="IWO38_000266"
     CDS             complement(25982..27004)
                     /locus_tag="IWO38_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ0312386.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            27132..27485
                     /locus_tag="IWO38_000267"
     CDS             27132..27485
                     /locus_tag="IWO38_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ0312387.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            27497..28663
                     /locus_tag="IWO38_000268"
     CDS             27497..28663
                     /locus_tag="IWO38_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0312388.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(28755..29375)
                     /locus_tag="IWO38_000269"
     CDS             complement(28755..29375)
                     /locus_tag="IWO38_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ0312389.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(29567..29863)
                     /locus_tag="IWO38_000270"
     CDS             complement(29567..29863)
                     /locus_tag="IWO38_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0312390.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            30067..30717
                     /locus_tag="IWO38_000271"
     CDS             30067..30717
                     /locus_tag="IWO38_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0312391.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            30733..32460
                     /locus_tag="IWO38_000272"
     CDS             30733..32460
                     /locus_tag="IWO38_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0312392.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            32457..34349
                     /locus_tag="IWO38_000273"
     CDS             32457..34349
                     /locus_tag="IWO38_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0312393.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            35053..35922
                     /locus_tag="IWO38_000274"
     CDS             35053..35922
                     /locus_tag="IWO38_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ0312394.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            36131..37156
                     /locus_tag="IWO38_000275"
     CDS             36131..37156
                     /locus_tag="IWO38_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ0312395.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            37195..38145
                     /gene="manA"
                     /locus_tag="IWO38_000276"
     CDS             37195..38145
                     /gene="manA"
                     /locus_tag="IWO38_000276"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ0312396.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            38239..39942
                     /locus_tag="IWO38_000277"
     CDS             38239..39942
                     /locus_tag="IWO38_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0312397.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            39944..41977
                     /locus_tag="IWO38_000278"
     CDS             39944..41977
                     /locus_tag="IWO38_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0312398.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            41988..42428
                     /locus_tag="IWO38_000279"
     CDS             41988..42428
                     /locus_tag="IWO38_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0312399.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(42628..43755)
                     /locus_tag="IWO38_000280"
     CDS             complement(42628..43755)
                     /locus_tag="IWO38_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0312400.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(43758..44435)
                     /locus_tag="IWO38_000281"
     CDS             complement(43758..44435)
                     /locus_tag="IWO38_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0312401.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            44699..46465
                     /locus_tag="IWO38_000282"
     CDS             44699..46465
                     /locus_tag="IWO38_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ0312402.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            46480..46917
                     /locus_tag="IWO38_000283"
     CDS             46480..46917
                     /locus_tag="IWO38_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0312403.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            46930..48081
                     /locus_tag="IWO38_000284"
     CDS             46930..48081
                     /locus_tag="IWO38_000284"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ0312404.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(48143..48841)
                     /locus_tag="IWO38_000285"
     CDS             complement(48143..48841)
                     /locus_tag="IWO38_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ0312405.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(48834..50186)
                     /locus_tag="IWO38_000286"
     CDS             complement(48834..50186)
                     /locus_tag="IWO38_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ0312406.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(50409..51809)
                     /locus_tag="IWO38_000287"
     CDS             complement(50409..51809)
                     /locus_tag="IWO38_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ0312407.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            51970..52707
                     /locus_tag="IWO38_000288"
     CDS             51970..52707
                     /locus_tag="IWO38_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ0312408.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            52832..53311
                     /locus_tag="IWO38_000289"
     CDS             52832..53311
                     /locus_tag="IWO38_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ0312409.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            53308..54075
                     /locus_tag="IWO38_000290"
     CDS             53308..54075
                     /locus_tag="IWO38_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312410.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEELVVYSRSQRRRHS"
     gene            54305..54874
                     /gene="lepB"
                     /locus_tag="IWO38_000291"
     CDS             54305..54874
                     /gene="lepB"
                     /locus_tag="IWO38_000291"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0312411.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(54978..55580)
                     /locus_tag="IWO38_000292"
     CDS             complement(54978..55580)
                     /locus_tag="IWO38_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0312412.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            55686..56155
                     /locus_tag="IWO38_000293"
                     /pseudo
     CDS             55686..56155
                     /locus_tag="IWO38_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            56261..56845
                     /locus_tag="IWO38_000294"
     CDS             56261..56845
                     /locus_tag="IWO38_000294"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ0312413.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            56848..57921
                     /gene="prfA"
                     /locus_tag="IWO38_000295"
     CDS             56848..57921
                     /gene="prfA"
                     /locus_tag="IWO38_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ0312414.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            57914..58753
                     /gene="prmC"
                     /locus_tag="IWO38_000296"
     CDS             57914..58753
                     /gene="prmC"
                     /locus_tag="IWO38_000296"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ0312415.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            58746..58925
                     /locus_tag="IWO38_000297"
     CDS             58746..58925
                     /locus_tag="IWO38_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312416.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            58947..59972
                     /locus_tag="IWO38_000298"
     CDS             58947..59972
                     /locus_tag="IWO38_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ0312417.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            60147..61391
                     /locus_tag="IWO38_000299"
     CDS             60147..61391
                     /locus_tag="IWO38_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ0312418.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            61544..62491
                     /locus_tag="IWO38_000300"
     CDS             61544..62491
                     /locus_tag="IWO38_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0312419.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            62624..63253
                     /gene="upp"
                     /locus_tag="IWO38_000301"
     CDS             62624..63253
                     /gene="upp"
                     /locus_tag="IWO38_000301"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ0312420.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(63392..63634)
                     /locus_tag="IWO38_000302"
     CDS             complement(63392..63634)
                     /locus_tag="IWO38_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312421.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            63755..63827
                     /locus_tag="IWO38_000303"
     tRNA            63755..63827
                     /locus_tag="IWO38_000303"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:63788..63790,aa:Thr,seq:cgt)
     gene            complement(63946..64584)
                     /locus_tag="IWO38_000304"
     CDS             complement(63946..64584)
                     /locus_tag="IWO38_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0312422.1"
                     /translation="MNNILGKKIELKKGILGTIVVVFGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(64707..65339)
                     /locus_tag="IWO38_000305"
     CDS             complement(64707..65339)
                     /locus_tag="IWO38_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ0312423.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            65690..65989
                     /locus_tag="IWO38_000306"
     CDS             65690..65989
                     /locus_tag="IWO38_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0312424.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            66484..68649
                     /gene="acm"
                     /locus_tag="IWO38_000307"
     CDS             66484..68649
                     /gene="acm"
                     /locus_tag="IWO38_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ0312425.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(68921..69406)
                     /locus_tag="IWO38_000308"
     CDS             complement(68921..69406)
                     /locus_tag="IWO38_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ0312426.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            69886..70527
                     /locus_tag="IWO38_000309"
     CDS             69886..70527
                     /locus_tag="IWO38_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0312427.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            70546..71181
                     /locus_tag="IWO38_000310"
     CDS             70546..71181
                     /locus_tag="IWO38_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0312428.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            71199..72014
                     /locus_tag="IWO38_000311"
     CDS             71199..72014
                     /locus_tag="IWO38_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0312429.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            72333..73769
                     /locus_tag="IWO38_000312"
     CDS             72333..73769
                     /locus_tag="IWO38_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0312430.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            73958..76405
                     /locus_tag="IWO38_000313"
     CDS             73958..76405
                     /locus_tag="IWO38_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ0312431.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            76635..77702
                     /gene="rlmN"
                     /locus_tag="IWO38_000314"
     CDS             76635..77702
                     /gene="rlmN"
                     /locus_tag="IWO38_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ0312432.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(77895..78998)
                     /locus_tag="IWO38_000315"
     CDS             complement(77895..78998)
                     /locus_tag="IWO38_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0312433.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            79551..80099
                     /gene="aac(6')"
                     /locus_tag="IWO38_000316"
     CDS             79551..80099
                     /gene="aac(6')"
                     /locus_tag="IWO38_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ0312434.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT        26821 a        13795 c        16506 g        23103 t
ORIGIN      
        1 cattcactaa taataaagat atatttacag tatcattata atgtattcaa tttaaaataa
       61 gagactttgg taataatgaa agaggaataa aacttttctt tggcaaaaca aactccttgc
      121 tatgagacgc gtttcataca ctatactcaa agtatcaaaa ggagggcttg caatgaatac
      181 ttatgtagag ataacagatg gcacaacgaa caattatttt tatgcgtttg tcgagttcaa
      241 gggatccgta ttaacgttgt acagttttca agggttgaat aggaatatag taaaagaaat
      301 accaatgaat gaaatcgaaa atttgacaaa agatatatat tggggcgggc agcggatcag
      361 tttttcgcat gaaggaaaaa tgtatcagtt ttttgaatgc ggtccagctg tggtagacta
      421 tttacaagaa aatctttttg tataggtggc gaagcagcag tggcaaacat tcgagatatc
      481 gcaaagctca caggttattc agtttctact atttcacgtg taatcaatgg acatccatat
      541 gtagatgagg aaaaaaggaa agaaatactt gcggtcatga aagaagtcga ttatatccct
      601 aatgcaaatg ccagacagtt aagttatgga aaaacgaaaa gcattggagt gattctgcct
      661 tataccaatc atccgtattt tgatcagttg atcagtggga tcattgaagc agcgtttgat
      721 tatgattaca aagtgacact tttaccgacg ggctatcata aagaaaaaga gcgaaggtat
      781 cttgaagaat ttgcagctaa agctttcgat ggattgatca ttacgtctcg tgccaataca
      841 ctagatgatt tattggcata tcaaaaatat ggacctctag ttttttgtga agaaatcaag
      901 ggaaaacagg caagctgtgt ctttattgat cgagaacaat caattacaga aggtcttact
      961 tatttgaagc agcaaggtat aaagcaattg ggcatcacgt tggggcgaag tggcaggcta
     1021 agttataact caaaaatcac tttgcagcta tgtcatcaac tttttccatc ttttgatgaa
     1081 tcacttgttt tttgggattg tatagatgca gagaaaggaa aacaagcagg cttctttttt
     1141 aaagagcttg gagttgatgg tgtctttaca aattcggata tggttgcagc aggcatcttg
     1201 caaagctatc tgcaagataa gacaccagtg gtgattggac gcgataattt gctgatcagc
     1261 gagcttcttg gtttcccaac catcgaccac catctggaag cttgcggaga aatggcattt
     1321 caattgtttc ttacagaaaa aaaggataaa gtgaaaatac cttatacgtt tattcaacga
     1381 tgaatatatt taaagtagga tgaaaaaact tgtgaaagaa tggagcagac accaaaagtt
     1441 agcataaaaa tctaacttgg gtgtctgctg ttttgttatt aggtcatttt acgtgtactt
     1501 ttttgttgga tcatgtaaaa gattacgcct ttttttggat aatttcattt aaagcttttg
     1561 ctgcaccatg accacttcgc tcgtcataat atgcagtgaa ccgttcatcg gcttcgtaca
     1621 tcattcctag tcctttatgc gcttttggct gatattcagg ccagctgtaa gagaaccact
     1681 tcttatgagc ttggaagata cggtctgcta agctttcatc ttctggttct tttaagtaaa
     1741 gagtgagatc aaataacatc tgcttttcaa cagtttgcat ttcttcatac gtttctttat
     1801 ccatattcat ccatttttca ttggatgctg taaccttttt ttttccgtat ttttgtcgaa
     1861 tctcttgtcc ataggattct tcattttctt tcaacttttg ttgtttaaac ccttcaaatt
     1921 tttctttgtc actcattttt cttcctcctt cgtacatatc aagtgtctgt tggacagttg
     1981 tcaagagggt atcgatttgt gcccgttttt ccagcaacat ttgctgatgc ttgattaaag
     2041 ctttctggtg gtcaaaagat ggattgtcta agacctcttg gatatcatcc aacttgaatg
     2101 ccattgttcg ataaaataag atgtgttgca gacgatcgac ttcttttttg ccataaatcc
     2161 gataacctga agaattgatc ctagctggtt ttagcagtcc aatctcatca taaaagcgca
     2221 aggttctcgg actgactcct gatatctctg acattttctt tattgtgtac tccattattt
     2281 ttcctcctga caaatgtaag cataatcctt tacgtaacga gatggtcaag aaaaaattgt
     2341 atttttttct cacgaaatta tgttatacta ttaagtgata aaaggtaaat ccttttgtct
     2401 ttaaaggttc aattgtaacc gattccttaa tgctaggaaa gatggtaaca taacttaaga
     2461 ttggatctaa atcgactata tagaaaagag gaatgagatg aagctaatcg aaacacctgt
     2521 gaatagtaat ttaaatatta agacatttta tcctaaagta gtggaattct tttttgggaa
     2581 tacggctatt aattattaca aattattttc gttagatcga acacaactgc tattggtaga
     2641 cacatatgat aaaaagcaag tcgtgatgat caatacgaag aaaaaaatca caaggcaaga
     2701 aatcgactat gcgattcatc acgttttaaa aatgacacga gaagatgtca aagtccatgt
     2761 aggagtgaaa caggagctag aacgtgccgg aatacaattc aaacgtccaa ataaagatat
     2821 tgtagtgata gaacaaaaaa atacgaagga ttctatttaa taacgactaa taatcatctt
     2881 tatggtaacc tgctccatat ctaatgtata caataaaaaa caaggcgaga ctgattgatg
     2941 tctcgccatt tttgtttcca ttctattgac agctgctttc ttactttgta tctttttaaa
     3001 aacgtgtgat attttttccg cgagtttttt caacgcaagg tatatcttgt cataattcgc
     3061 ctttccaaaa aaattcaata cataataagt tatatccgtt tctttgggga taagaagcaa
     3121 aaaaggaaag acgtattatt gcttcaaaaa cttttgccat tgattatgtt gtagagacat
     3181 ttggttatat ttgttcaatc atcggtatct taacggctac gattgtagta ggaaaatcta
     3241 cttattttat caagtaagaa aaatagaaaa cgctaatttc ttcgtgtctc ggatacattg
     3301 aataaaataa gtattctttc tcgtttaggt taaataccaa taagaaagga tagttttagc
     3361 agatggagtt catttttagc aagaaatatc cgtaaaacat gtgctttttc agaaaaaatg
     3421 gaagatttct tttcctatta attttttttg ttataatggg taggaaatga aaacttgcca
     3481 agaggagtaa tacaagatga ataatactta cccagacgac agtttgacgc tgcatacgga
     3541 tctatatcaa atcaatatga tgcagacata ctgggaatta ggtcgagctg atttacatgc
     3601 tgtttttgaa tgttactttc gagaaatgcc ttttaaccac ggatacgcgg tttttgcagg
     3661 attggaacgg ttagtcaatt atttagagaa tctgacgttc aatgattccg atattgctta
     3721 cttgcgtgga ttagaagtct atcctgaagg ttttttggag tatttacaaa attttgaatt
     3781 taaagcaaca gttcgttctg ctcgtgaagg agaactagtt tttgcaaatg aaccattaat
     3841 ccaagtggaa gggcctttag cgcactgcca attagtagaa acagcactgt tgaatatggt
     3901 caatttccaa acgttgatcg cgacaaaagc agctcgtatc aaatctgtca tcggagaaga
     3961 tccactattg gaatttggaa ctagacgggc acaagaactg gatgcagcag tctggggaac
     4021 tagagcggca tatatcggcg gagcagatgc aacaagcaat gtacgtgctg ggaagatatt
     4081 tgggatacca gctagcggga cccatgctca ttcgttagtc caatcttatg gaaatgatta
     4141 tgaagcattt atggcctacg ctaaaaccca taaagactgc gtgttcttag tcgacactta
     4201 tgatacgttg aaatcaggtg tgccaagtgc gattcgcgtg gcgaaagaat taggtgacaa
     4261 aatcaacttc caaggcgtac gcatcgacag tggagatatg gcctatatat ctaagcgtgt
     4321 tcgggaacaa ttagatgcag ccggatttac ggaagcaaaa atctatgctt cgaatgactt
     4381 ggatgaagca acgattttga atttaaagat gcaaaaagct aaaattgatg tatggggtgt
     4441 tgggacaaaa ttgatcacag cctatgatca gccagccctt ggtgcagtat tcaagctagt
     4501 atcgatcgaa gatgatgaag gaaaaatgat ggatacgatc aagttatcaa gcaatgcaga
     4561 aaaagtaact actccaggga aaaaacaagt gtggcgcatc actcgaaatt ttgatgggaa
     4621 atctgaagga gactacgtga cattatggga tgaagaccct cgagaagaag aagctatctt
     4681 catgttccat ccagtgcata cgtttatcaa taaaactgtt cgagacttta cagctcgccc
     4741 agttttacaa gatatcttta tcgaaggaaa aagagtttac gaattacctg cactaaacga
     4801 aatcaaagaa tatacaaaag aaaatctaga ttcactatgg gaagagtata aacgagactt
     4861 gaatccgcaa aaatacccag tcgatctgtc tactgaatgc tggaaccata aaatggctat
     4921 catggaacga atgaaaaaaa gcgtagcaga acttcataca gaagcatagg aggagaataa
     4981 atgagcctgc agacagaaat tatcaatgaa ttaggtgtca aaccaacaat tgatccaaaa
     5041 gaagaaatac gtaaaagtat tgactttatg aaagcatatt tgaaaaaata tccgtttctt
     5101 aaaacttttg tactaggtat cagcggcggc caagattcta ctttagcagg ccgtttggcg
     5161 caactaacga tggaagaaat gcgtgaagaa acaaaagatg aaaactatca atttatcgct
     5221 gttcgtcttc cttatggtga acaagcagat gaagaagatg caaaagctgc tcttgacttt
     5281 atccagcccg atgttagtct gcgagtaaat atcaaaccag ctgttgatgc acaagtacaa
     5341 gtattaagcg aagcaggtgt gggaatcagc gattttaaca aaggaaatat caaagcacgt
     5401 caacgtatga ttacgcaata tgccgtagct ggagaaagag caggagccgt attagggaca
     5461 gatcatgctg cagaaaacat tactggattc ttcacgaaat ttggtgatgg tggagccgac
     5521 attttgccat tattccgttt ggacaaacga caagggaaac aattgcttca agcattgaat
     5581 gcaccggaaa aattatacac aaaaattccg actgcagatc ttgaagatgg gaaaccaatg
     5641 attgcagatg aagtcgcact aggtgtcaca tataatgaaa ttgatgatta tctagaagga
     5701 aaagaagttc ccgctcaagc acaagaaaaa atcgaagcat ggtggaacaa gacacaacat
     5761 aaacgacatt tgccaatttc cgttttggat gatttctgga aataacaata aagcaaaaac
     5821 agccgtccct tcatagggat tggctgtttt tgttttataa aatagaaaag aaaaatatca
     5881 gctgtagtaa acttttaatt tgatatcctg atcgtaatta ccgaatcctt taccaaataa
     5941 agtacagccc cccacatgtt tggcatcgtc tttcacttcg attcgcaaat gccaagtgtc
     6001 ttgctcttta gggatgtcgt tgatcgtgat gtttgacaag ggttctccat tcatattcgt
     6061 caagtgtttg gtaattcgga tcgtcttcag caagccgtat tgattaagat tatctggata
     6121 ccaatcaggc gtatatttac cgcggatatc agcaaaatct ccagggctcg tccatgttcc
     6181 tagctccaca ccatttaggc tgaaagtgat atctgaaggc caattgtcat ttgaaaaagg
     6241 aaattcagaa gaaatttcca cagaaacttc caacatctcc aatgtatcct ctgtatttaa
     6301 aaaattaggt gcctgatact cgacaaatcc gctggtaaac cagagaatgc gggcatccat
     6361 ccttcgagga tccataaaat agcgtggttc atccactttc ccgataaaat catggatcgt
     6421 agctaaaccg catgttggtt caattgcata atttgtatag tgaccaatag gtatcgatgt
     6481 ttctttcgta tcaaaagcat tgaatatttt ttctggaaaa ctgatatcga ttttatcgac
     6541 acgaaggctc gaaatttttt gttgtcctac tttttctgtt ttgatgatgt ttgcttcctc
     6601 tagctttttc acgtgcatcg tcgtgatagc actgctcact ccaagttctt ttgccaaatc
     6661 tttgacattc atcttttttt tggaaagtag ctggatgatc ttgatccttg ttttactagc
     6721 taatgcttca tataccggta aggattcttc gtttatttgt agttgcatat atatcacctc
     6781 ttaaaaggtt tttcttatat attaacatat ttgttaataa aagttatatt aaattatttg
     6841 ttttaatcat tattaatatt ttggttaatt aaaatcgata aaaaataagt aagtttaaca
     6901 tttatattat tgacaacgct ttcaacgagg tgtattattc atgtataaat taaaggaggt
     6961 cagttatgga agccacaatg ttgattagta gtgtcaagcg tgtaggcaaa gttgatccgc
     7021 ggttgtacag ttcatttatt gaacatatgg gacgagcagt atatgaaggg atttatcagc
     7081 cagatcatcc atcagctaat aaaagtggat ttcgccaaga cgtcattcac ttggtaaaag
     7141 aattgaacat tcccatgatt agatatcctg gtggaaattt tgtgtcagga tttcgttggg
     7201 aagacagtat tgggcccata gcaaatagac ctagaaggtt ggatttagct tggcggacaa
     7261 ttgaaacgaa tgaagttggg attcatgaat tttataactg gtgtcagcat gtgggggccg
     7321 aaatcaatat ggctgtcaat ctgggtactc gtggagtaga tgcagctcgt aatttagtcg
     7381 aatattgtaa ttttcctaaa ggaacttatt ggagtgattt aaggcgttcg aatggtcaag
     7441 agagcccttt tggaattaag acatggtgtt tagggaatga aatggacggt ccttggcaga
     7501 ttggacataa aacagcggaa gaatatggac ggttagcaga ggaaacggct aaggcgatga
     7561 aactcgtaga tgattcaatt gaggtggttt tatgcggaag ttcgaatcgg caaatgccta
     7621 catttggtga ttgggagtta acagttcttg atcaagcgta cgatcaaata gattatttgt
     7681 ctctgcatca atattatggc aaccaaaaaa atgatctttc tcattatctt gcacgttcct
     7741 tagacatgga tcggttcatt tctgaagtga cagcgatgtg cgatacagtc aaggcgaaga
     7801 aacatagtaa gaagcagatc aacctgtcct ttgatgaatg gaatatttgg tatcattcag
     7861 cggaacaaga tgagcaagca gcgccttggc agattgctcc tcctttacta gaagataact
     7921 ataattttga agatgcatta ctattaggct gtctattaat cactttattg aaacatgcag
     7981 atagagtaaa gattgcttgt cttgctcaat tggtaaatgt tattgcacca atcaggacag
     8041 aaacaaacgg agaagcttgg agacagacga tctattatcc atttatgcaa gctgctgtgt
     8101 atggacaagg agaggtgtta agcccacaaa ttcgttcagc aaactatgcg acggaagatt
     8161 ttgaagaagt gccgtattta gaaagtatag ccgtaataaa tgacaaagaa cttgtcatat
     8221 ttgcagttaa ccgtggagaa gaagaaatga atctaactgt tcacttgtct gaattatcat
     8281 tagagggagt gattgatttt tctgaaatga gtggatttga tacaaaagca actaatcttc
     8341 ctaaaagtga acaagtaaaa ccaagtcatt caacaaatgt aaaagctgaa gatgggaaaa
     8401 tcaattgttt attgaaaccg ctatcttgga atgtggtcag atgtcgttta tcagatgaag
     8461 gaggaacaaa aaatgaaagg taaaaaatgg ttaattggct ctttgttggt agctgcaaca
     8521 ggtattttag gagcttgcgg tggcggaagt tcagcagaat ctgattctga taaagaagtg
     8581 actttgactt tttggaatgg gtttactgca tctgatggtg aaatattgca agaaatcgtt
     8641 aatgaattta ataaaacaaa tgataaaaac atcaaaattg aaatggatat catgacttgg
     8701 gccaatttga atgaaaaact tcctacagcg atttcttcta aaaaagcacc tgactttatt
     8761 gcattaaatt atccagattt tgcacagtat gtagaaaacg gagcggtaca accattagac
     8821 gatttctggg aatatgatgg agtagataaa tctgatttta aagaaacggc tattgaatta
     8881 ggtgaagtag aaggtaaaca gtattttgtc cccatgcagg tacaaggcat gtatatgtat
     8941 tggaataatg atttgtttaa acaagctgga ttagatactg aacaaccacc tactacttgg
     9001 gaagaattga cagaaatggc accaaaatta actgatagta gtaaaaatgt atcaggtttt
     9061 gtcttcaata aggatggaac tgcaccgtta tataactgga tgttagcaaa tggcggtaaa
     9121 ttagtgaacg atgattatac taagtctgaa tttgcgtctc ctgagaacct taaaacgtta
     9181 aaagcgatcc aaaaaatgat ccatgtagac aagactggtc cagaatcgat ttctggcgcc
     9241 gaaatggaca atttaatgaa tgctggtcaa ctagccattg aaatcaatgg gccatggtta
     9301 aataacggat tgaaagcaaa cgagattgat tataatgtga ctaccttacc acaagggagt
     9361 gattcagaca aaaaaacagc tattttagat ggcgtaggat atgccattcc ggcaagtaca
     9421 gataataata aaaaagaagc tgtctatgag tttttgaaat attggaatac aactgaaatt
     9481 ggtaaaaaat ggagtatcga aaatggtttt cctgcctact tggattcagt agcaaaagat
     9541 gaagaagtaa aaaatgatcc tattgtatct gaattatcga aacaaatgga ctatgcagag
     9601 ccatatttac caggttttac ccaaatctct gcgatcaata acgatatcat caatccactg
     9661 attgaaaaat tattagcagg agaagatcct gagacactca tgaacgatgc agatgatgcc
     9721 atcaatcaat tactgcaaaa atagtacaga actttataca ggatagactg ttggcaagat
     9781 acagatatct gaatgatcga cctagtattt gcagataggt gttattgcaa aaatcacagc
     9841 gattgttcgg atatcttatt cagattcgtt taaaccaaga ttgtatattt tatcaaaatg
     9901 aaagatgagg tggactatat gaacggattc accaaatatt ggaatcgtcc tgatcgtgct
     9961 gcgtatttgt ttttaactcc atctttttta gttttattat tatttactgt cataccgctt
    10021 ttaggaacat ttgctattag ttttacagat ttgaatattt tctttacatc aagaaatttt
    10081 gtaagttttg aaaatttcac acgtatattt agtgatgaac gagcagttaa ttcgttgatt
    10141 cattctttat atttcaccgt actcgaaacg ccaacacaaa ttatcgttgg tttattagca
    10201 gcagtagcat tatctaaaaa taccagattc aatcgttttt gtcgttcgac attttacatt
    10261 ccagttattt gttcattgac gtcaattgcg attatttttt ctatgttgct tgatccgaat
    10321 gtaggggcga ttccttactt gttttcacaa gtcggcttgc cgatccctca attttttcgc
    10381 gatccaaccc ttgctatgcc gacggtagcc atcatgactg tttggaagaa ttttggtgtt
    10441 actatgacga ttctcttgac tgctattcag ggaatctctc cctctttgta tgaatctgct
    10501 caaatggatg gtgcaacaaa tcgacagcag ttttttaaca ttacgctacc tcagatcgtt
    10561 ccctccttag gtttttgtat tttaacgaat ctgattggtt cgatgcaagt gtttgaccaa
    10621 gtatatgttg caaccgctgg tggcccacaa tttaagacag aaacagctgt tcaatatatt
    10681 tacagtcgag gatttacagc accttatgaa ttgggctatg catcagcact gtcttcggtt
    10741 ttgtttatca ttgttgcaac tatagctatc tccactaatg tgtacatgtc acggaaagaa
    10801 aaacaaatga aataggaggg aaacctgtat ggcaccagaa agctcgatta cagttactgt
    10861 gaatagtcga aaagcagtta gtggtaaaaa aatcattcga acgattagta tgctagttat
    10921 attgttggtt attgcgtttc cttttttgtg gctgattatt tcttctttta aacacgaaaa
    10981 agacattatc tcatttccgc ctagaatatt tgcagatagc tataccctag ataactatat
    11041 taaggtttgg acaacgatcc ctttattaga ttacatcaaa aatacagtga tttttgctgg
    11101 agggacagtg atcacttctg tattctttga ttctcttgcg gggtatgcat ttgctcgtat
    11161 gcgtttcaaa ggaaaatcag ttttgttcta tttcgttttg ctgacaatga tgatcccatt
    11221 ccaagtcttc atgattcctc tatttattga agtcaatttg cttggcatgc ttgatactta
    11281 tgcaggtttg atcattcctc gtatgacaac tgcatttggt atcttcatga tgcgttcctt
    11341 ttttattact ttaccagatt ctttggaaga ggcagcacgc attgatggat taagtgagtt
    11401 taatattttt ttgaaaatca tgttgccatt gagcaaacct actcttttat cattagggat
    11461 tttcacgctg atgaatagtt ggaatgactt gctttatcca ttgatattga caagtagttc
    11521 aaaaatgaga actttaccag cagggttagc tttatttaca gggcagaaca tttcttttta
    11581 tggacctgta atggctggaa cggttatttc aatgctgcca ttactagtgg tttatatttt
    11641 tgcacagaaa tattttgttc aaggaacagc tatgtctgga atgaaagagt gaggaagatg
    11701 aaaatagttg agactaaact ttatggacag ttgattgtgg tttctgatta catcttatta
    11761 ggtattctct gggtaatcgt gtgtttgcct gttattacta tcgtgcctgc tacatgtgct
    11821 gttttttatg taatggcgca atggaaaggt gagggatcag gaaatatcat ttattttttc
    11881 tttcaaggaa taaagcaaca ttttggtaag aacttgttcc ttagtctgtt aacgatcatt
    11941 atttattttc ttgttaatca attattagaa gaacagacaa tcgtactttc aattagtgga
    12001 tatgtagttt tacttattta ttccatgttt cttttatcct ggatatatgt gatttctgaa
    12061 aacaaagata tcacattttt acaaattttt gaacaaagtg catggaaagt attttttgat
    12121 tttttgagaa atattctttg ttgcgtattg ttattaggat ttgtactgct catatttctt
    12181 ttccctcctt ttatttttat ctttgctggt ggcgtatgga aacttgtatc tattatcttg
    12241 actataggaa atgaaaggta gggaaagctg ttggacgaag aagtgaaagt aaaaatcaga
    12301 gaggatttta cggtaggaga gatcagttct cggctgtttg gctcatttgt tgagcatatg
    12361 ggaagtgtcg tctatacagg gatttatgaa ccgggccatg aaacagctga tggtgacggg
    12421 tttagacaag atgtcctctc ttatgtaaag gaattagaat taggtgtcat tcgctatcct
    12481 ggaggaaact ttacatctgg ttatgattgg gtggatactg taggtaggaa agaacaacga
    12541 ccaaaaaaaa ttgatttagc atggcgaggt atagaaccaa attcttttgg actccacgaa
    12601 ttttttaaat ggatcagtca tgtacaagcg aaaccgatat tcacggtgaa tttaggaaca
    12661 aaaggaattg atgaagcaaa aaatttagta gaatattgta attttccaaa aggaaccttc
    12721 tggagtgatt taagaataaa aaatgggcaa aaaaagcctt ttggcgttaa attatggtgt
    12781 ttagggaatg agctagacgg tccatggcaa gtgggggcta agagtgctta tgaatatggt
    12841 aggttagcaa atgaagcctc aaaagtcatg aagttagtgg atgattcaat tgaaacaatc
    12901 ttagttggaa gctctacgcc acgtttacca tcgtaccctg aatgggatcg tatcgcatta
    12961 gaagaagctt atgagaatgt agattattta gctttgcata actatattga taagtatgat
    13021 gaagaagact tgactaaacc tcctaaaaat gagcgtgatg atacagcgac ataccttgca
    13081 aaagcttatc gattcgatcg tcaaattgaa gaaatcgttg ctacttgtga ttatgtaaaa
    13141 ggatatttga gaagtgagaa aacaatgtat ctagcgtttg atgaatggaa tgtacatgcc
    13201 caaccggaaa aaagacatag ggattttgaa gtcggtagtc ccatcgattg gtgttacttt
    13261 actatggaag atacattatt atttggttca ttagggctgg ctattttacg acacgcagat
    13321 cgcgtaaaga ttagttgtca atcattgtta gtaaacacaa tacctttaat actgacagat
    13381 gctaaaggac aagcatggag gaatccaact tattatgtga tgcaacactt atcgaaatat
    13441 gctaaaggtc aagtattgaa acaacaaatc atttgccctg tttatgattg tgaaaagata
    13501 aaaaatgttc cttatgtgga cagtgtgatc gtagataacg gcaatcagct gactattttc
    13561 ttgatcaatc gcacaaatga acaacaaaag ataacgattg aacatgattt caatttgcta
    13621 gatcaaggag agcactacac attaaccagt agcgatctgt tggacaaaaa tacacgtgaa
    13681 gaaccagaaa aaataaaacc tgaaataaac tatttttcaa tagaaaaaca tgacgaatta
    13741 aatgtaggga tagaaaaata ctcttggaat gtaattagaa taaagaaaat gtgaggaaat
    13801 ttgttatgat gaagacactt tataataatc ctatcgttcg gcaacgggca gatccttggg
    13861 tatataaaca tacagatggt tattattatt ttacaggatc ggttccaggc taccaagtga
    13921 ttgaattaag aagggcaaag tcactgaatg aattagaaca tgctgcaagc ctgattgtat
    13981 ggcaggctca tgaagaagga ccgatgagtg aattgatctg ggcacctgaa gtccattata
    14041 tcaatggaaa atggtatatt tactttgctg caagcaatca taaaactata cgtgatgaat
    14101 ctcatcatca ccgcatgttc gtacttgaaa atcagcatat agatccaatg aatacagatt
    14161 ggatagaaaa gggacaaata aaaacgaaaa gggaaacttt cagtttagac ggaacaactt
    14221 tctcttctca aggaaagcta tattatgtgt gggcccaaca agatcctgaa attactggga
    14281 attctaatct ttatctatcc gaaatggata atccttggac gttaaagggg aagcagatcc
    14341 tcctttctat acctgagtat gaatgggaaa aacggggttt ttcagtaaat gaaggtccag
    14401 cagtccttac tcgcaatggg aaaatattca ttacgtattc tggtagtgca acagatgaaa
    14461 actatgccat gggcctgctg tgggctgatg agcacaagaa tcttttatta aaagaatcat
    14521 ggcataaatt atcggagcca gtattcgttt cttctgaaaa aaataagcaa tttggtcctg
    14581 gtcacaactc gtttacggtt tcagaagacg ggtgctttga tattttgatc tatcatgctc
    14641 gaccagagaa gaaccaaaaa ggggatccgt tagacaatcc taatcggcac gcgaacgcgc
    14701 aatgctttac gtggaatgag caaggatttc cagaatttgg tgaacctgtt ccgtatgcct
    14761 tatgagcaca agaaaccttg atttttgctt gaataaacct tgcagactcg ttatgatata
    14821 tgtacgtaca aataaaaagg ggcaaggcaa atggcaaaat atcaagatat tgcagaagag
    14881 ataaaacaaa agatccttcg tgaggaatac gaagtaaata caaccattcc ctcagaattc
    14941 aagctgcaag aaatatatga cgtcagcaga catacgattc ggcaagcaat cgctgtgttg
    15001 gtaaatgagg gctatctgcg taaagaaaaa gggtcgggca catatgtaga tgacagctat
    15061 aagaaagaaa aaacaccatt gaacagaaca aaaacgatcg gtgtcatcac cacatatctc
    15121 tctgattata tttttccgtc gattatccga ggaatcgaaa atgaattacg agaatctggg
    15181 tattcattgc ttttagccag tacgaataac gatcacgaat tggagaaaga gtgtttgaag
    15241 aaaatgattg cttatggggt agatggtttg atcgttgagc ctacgaaaag taatcagtat
    15301 aacccaaact tagcacttta tgttgctttg agagaacaag gcattccgat ggtgatgatc
    15361 aatgctgctt atgaagagtt aaatgcctct tatatttgtt tagatgatgt gaaagtcggt
    15421 tttatggcag cagaatattt gataaaaaaa ggacatcagc gtctgttgtt catcacaaaa
    15481 atcgacgatc ttcaaggaaa atacaggatg aaaggtttta tcagcgcgtg tgaacagtat
    15541 aatgtgcgtt ttttgacgga agatatcatt actttcacca cggaaacacg agaaacaatc
    15601 gctgaaaaaa cagtacaacg gttgaaagaa gaacaagctt tagatggcat catttgctat
    15661 aacgatcaaa tagctagttt aatgctagat aagttgacag aaaatggcta tcatattcca
    15721 gatgacctat cgatcgtagg aacagacgat tcttctttaa gccatttagg gaataaaaaa
    15781 ttgacaacat tgacgcatcc aaaagaaaag ttgggacaag atgccgcacg gtggatcgtc
    15841 acaacgattg aaaaaggaca ggcacagcca aatattctct atgaacctgt actagttgaa
    15901 cgggaaacag tgaaagaaaa cgaaaaataa gagacgggtt tttaccgcct cttattgctc
    15961 ctacggttac tcgtcgcaat gtttgtgaga gcttttgtca tcgctcaagg ctgaacgttt
    16021 ctttcaccac ctctccacta aacggtgttc tcctaccaac tggtcacaag tcacaatatt
    16081 catcaaacat gagaagctgt tttttgaaat tgttccttgt gactggaaac taagcggttc
    16141 cttcttattt cttgaagctg accacttctg tcacaacctc tttaagaatg atggatagaa
    16201 atacgtttag gataagtttt tttagatgca ctttgttcga tttcctctaa agatttccca
    16261 cgagtttctg gtacgatctc ttgaataaat aaaaccccta aaaagcagat gatcccaaaa
    16321 atgccgaaaa cgatttcttg agacatagaa gcagtcatca caggaaaaag caagccaact
    16381 aggaaagaac caatccaatt aaaagaagag gctaggccag atgctcgccc gcggacagct
    16441 agtgggaata tttcgcctac gattacccat gtcagtggag cccatgtgca agaataaaat
    16501 gcgacataaa tacataaaaa caagagaatc aacaacgaat ttgtatgagc atccagtaca
    16561 gttcctaaaa cagctggtag gatgaaagaa agtcccatca cgctaccgcc tattttcaat
    16621 aaagttcgcc gattgaactt gtctgcaata accaaaaaga gcaaagaacc agcaactaaa
    16681 atgattcctt ggatgatcgg ccacatcaat gcatcgctcg ctgcatgccc agtcgctttt
    16741 tctacgatca gcggaatata gtaaaaaata gcattagctc cttgaaattg ctggaatact
    16801 gctacaccaa taccggcaat gactaaggaa cgatattttt ctaaaaacaa agtatgccaa
    16861 gaagttccgg ttgtcttttc ttgtttgaca ttcagctgga tttgttcgaa ttcctgttca
    16921 gcttcttttg gtgtccgaat atagttcaat actctttttg cctcttccaa gcggccagat
    16981 tgcataagaa aacgcggtga ctctggtaga gctaaaacac cgaaaaataa aatcagcgcg
    17041 gggacagcag cgagccctaa catcacgcgc caagccatcg tttcaggcaa cccttttagc
    17101 aaataatctg caacatagga aaggagcatg ccagaagcaa tcatcacttg attgatccct
    17161 gacaatcttc cccttagtct agcaggagcc atttcagaca tataagcagg aactagggca
    17221 gatgcagcgc caacagctaa acctagaaga atccttgctg caatcagaaa ataagaacca
    17281 ttgtgagggg cagatgcaga aaggacagaa ccagccataa aaataagtgc agaaagaaga
    17341 atcatttttc gtcgtccaag cttatcagaa agaagaccag ataaagagcc gccaaggatc
    17401 gcacctaaca tgaccgaaga cgtgatccat cctatcaaag aagctttacc tgccaaaccc
    17461 caatcatgct gtaaaaaagg taaggcgccg gtcataacac caatatcata tccaaataga
    17521 atgccgccaa atgcgccaaa aaaataaata aacgatgagg aaactttttt ttcttttttc
    17581 ataagtattc tctccttcta gaatttcgag aaggcggttg atcagatttt gtaagcaatt
    17641 tcattttata acttatgtac gtacaaaaca agtgtttttt attgtattct tgtaaataac
    17701 tgcttgacat gtacgtacaa aagtatacaa taatcattgt aagcgctact aaaaagacgg
    17761 tttgatcagg tttgacgaga aggagggagt taaatcatta tgaaggaagt aaatgcgcaa
    17821 agtatgttag atgtcataga tggtcgtaca tctttaggga ttgagtttgg atcaactagg
    17881 atcaaagctg tattgattga ttcatctttt actcctattg ccgctggtag ttatgagtgg
    17941 gaaaacaaac tagaaaatgg tatctggacc tattcattag aagatatttg gaaaggactg
    18001 cgcgtcagtt accgaaaaat ggcagcagaa gtattcgaaa tctatggcga gaatcttacg
    18061 acaatcgggt cgattgggtt cagtgcaatg atgcacggtt atctagcatt tgatagcaag
    18121 gaacagttac tagtaccatt tcgtacttgg cgaaatacaa tcactgaaga agcagaacga
    18181 atattaacgg aaaaatttca atacaatatt ccgcagagat ggagtatcgc tcatttgtat
    18241 caatctattt tgaataaaga agaacatgtg aaagaaatta attttttgac aacgcttgca
    18301 ggttatgtcc attggcagct tactggtgag aaagtactag gcgtaggaga tgcatcaggc
    18361 atgtttccta tcgatccttc aacgaaaacc taccatcaag aaatgaaaaa tatttttggt
    18421 cagttaatcg aaaaagcgca cgtagcgatt gacttggatc acattttgcc taaagttcta
    18481 gtagcaggag aagaagctgg caagttaagc gaatcagggg cttatctgat cgaccctacg
    18541 ggaaatttga gaccaggaat cccggtttgt ccgccagaag gggatgcagg aaccggtatg
    18601 gtcgctacga acagtgtagc caaagggaca gggaacattt cggctggtac ttccgccttc
    18661 gctatgatcg tattagaaaa agaactagaa aatgtccatc cggaaatcga catggtcact
    18721 acaccttcag gagaactagt aggcatggtt catacgaata actgctcttc ggatatcaat
    18781 gcttgggtca gtatttttga agaatttacc aattctttgg gtatggagat aagcagagat
    18841 aaattattca cggtattatt caacaaagcg ctagaaggcg atacggattg cgggggatta
    18901 ctttcttatg gttatttttc cggtgaaaac attacaggag taaatgaagg aaggccatta
    18961 tttgtacgta caccaggtag ccgttttgat ttagcgaatt tcatgcggat ccatttagcc
    19021 agtgcttttg gtgcaatgag aatcggaatg gatatattga aatctgagaa tgtccaaatt
    19081 gatcgtttag tggggcatgg ggggattttt aaaacaccag aagttggaca gcggattcta
    19141 gcatctgcta tggaggcacc agttacagtg atggacacag ccggagaagg tggagcgtgg
    19201 ggaatcgcgt tattagcagc ctatatgatg gatcgacatg ggaagtcttt agaaaacttc
    19261 ttggcagaag acgtgtttgg acaagaccaa ggaatcacca tcacaccttc agcagaagaa
    19321 atagcaggct atcagatatt tatgagacgt taccgtgagg ggatcgacat tgaaaaaaca
    19381 gcgattcatc aattgcaaga aaaaggagtg atagaaaatg ctggaacaac tgaaagaaga
    19441 agtataccaa gcaaatcttg acttgcctaa acacggactc gtcaaatata catggggaaa
    19501 tgtgagcgct tttgatcctg aaacacgata tttcgttatc aaacctagcg gagtcagtta
    19561 tgaagagatg acagcagacg acatggtcgt tgtggacttg gacaatcata tcattgaagg
    19621 taaactcaat ccctcttcgg atacgcctac tcatgctgtt ttatatcgtt catttcctca
    19681 aatcggagga atcgtccata ctcactcgac atgggcgacg atctgggcac aagcaggatt
    19741 ggatgttcct gcgatgggga cgacgcatgc agatactttt tatggatcgg taccttgtgc
    19801 tcgatttttg acacaacagg aaatcgacag cgggtacgaa tatgaaacag gaaaagttat
    19861 catagaaaca ttcaaagaac gcaaaattga tcccttagca attccagggg tacttttaca
    19921 cggacacggt ccatttactt gggggaaaga tgcgcaaagt gcggtaatga acgcagtcgt
    19981 actagatgaa gtatgcaaga tgaatttatt taccagacag ctcaattcat tttcagaaga
    20041 gctgcctcaa cggattttag ataaacatta tttacgaaaa cacggaaaaa atgcgtatta
    20101 cggacaaaac aaataaaagg agtggctaat tatgttgaat attggagaaa aagaattttg
    20161 gtttgttgta ggatcacagc atttatatgg ggaagaagca ctgcgagaag taaagaaaca
    20221 tgcacaagaa atggtcgatg aactgaatga aaatggccaa ttgccttatc caatccgcct
    20281 tcaagaatta gcagttactg ctgatacgat cacaaaaatc atgaaagagg tcaattatcg
    20341 agaagaagta gcaggtgtga tcacttggat gcatactttc tcaccagcaa aaatgtggat
    20401 ccgcgggaca aagcttctac aaaaaccatt gcttcattta gctacgcaat ataacgaaag
    20461 cattccttgg aaaacgatcg atatggattt catgaattta aatcaatccg ctcatggtga
    20521 ccgcgaatat gggttcatca atgctcgatt aaataaacaa aataaaatcg tcgttggcta
    20581 ctggaaacga cctgagatcc aaaaagagat tgctgattgg atggatgttg cagtagccta
    20641 taatgaaagt tttggaataa aagtggctcg ttttggtgac aacatgagaa acgtgggtgt
    20701 cacagaagga gacaaagtgg aagcacagat tcaatttggt tggactgttg attattttgg
    20761 tatcggagat cttgtacaag taatcgatcg cgtatcagac gaagaagtcg atcaattatt
    20821 tgaagagtac aaggaattgt ataccttcga ctatggtgat tatgaagaaa agacatggga
    20881 ggaacatgta aaagtacaag ctcagcagga aatcggtatt cgccgtttct tagaagaagg
    20941 aggctataac gcttttacga ctaattttga agacctgtat ggaatgaaac aactacctgg
    21001 actagctgta caacgtttga tggcagaagg atacggtttt gctggggaag gcgactggaa
    21061 aactgcagcg attgatcggt tgctgaaaat catggctcga gggaaagata caggattcat
    21121 ggaagattat acttacgagc tagctagtgg ccaagaagca atcttggaat cccatatgat
    21181 ggaagtagat ccgacgcttg ctgcgacgaa accaagaatc gttgtttctc ctttatcaat
    21241 gggagataga gaagatccgg cgcgtcttgt atttgatggg aaagcaggag aaggggtagt
    21301 cgtatcgatg gcagatttcg gtacgcatta taaactgctg atcaatgaag tagaagcctt
    21361 tgaaccaacg actgaagcac ctaatttacc tgttgcacgt gtgttatgga aaactaaacc
    21421 gaatttccat gaaggtgtcc attcatggat tcaggcaggt ggcggtcatc ataccgttgt
    21481 ctcgctaaat ctaacaacag atcaaattga aacgtgggca aaattagttg agttggaaac
    21541 agtggttatt agataaaaag aggttgtgaa aaagccactt agttttaaga aataagaaga
    21601 gattgacgaa atttgctttt caactttttt gaaatttagt cttattatca atgtcagcta
    21661 acggaacacc atttatcaag atatctcacc ctcaatattt tgctctcgaa aataaggctt
    21721 atggcagtaa cttgtcatag ccttattttt atcactcagc ctactctgct ctaactgctc
    21781 tttttgcttc taggaagtca aatggtaaac agttgtctaa ttgcagaaat ttcgagatac
    21841 ttgttagtaa ggagggggtt ttatgtatca cgttcatttg attgatgcta ttccagaaca
    21901 agagatagaa aagttacaag gggaatattt caaaataata gatgatttgc cagccgaagg
    21961 aatagttatt cgtagtacga aagtgaaaga tgaatggctt acaccagatt tgctagcaat
    22021 ttcaagagct ggtgttgggg tgaacacgat caacgttgaa aaagcttctg aaaacgggac
    22081 gatcgtcatg aatacaccag gtgtaaatgc taatgcagta aaagagcttg ttttgtgctg
    22141 tttgctctta tcttcccgtc cgataatcga agcaagtcgt atggtacaaa cactaacagg
    22201 tcctaatatt ttagaacagg cagaaaacaa aagaagtgct tatgtgggac gagaattgca
    22261 aggcaaaacc atcggtcttc ttggtcttgg agcgataggc acaaaagtag cgctaagttg
    22321 ctatagctta gggatggatg tcttgggtta ttctatccgc gatgcccagt tggattatgt
    22381 tcggcaagca gatttagaaa cagttctttc tacctcggat tacatcgtag tcatgctgcc
    22441 gttgacagaa gatacaaaag gattgatcga tcaggcgaat atagaaaaaa tgaaaaaaga
    22501 tgctgtactt atcaatgtag gaagaagtga aattgttgat aaatatgctg ttatgcaagc
    22561 attggaaaaa caacatctgg ctaaatactt gactgatttt ccagaagaag agttcttaga
    22621 aaatgaccgt atacttatgt tgcctcatct aggggatcga ctcaggaagc tttggcagac
    22681 agcggacgtt tggcagttga agccttaaaa gattatcttt tgttcggtac ggttagagaa
    22741 gcggtcaatt atccatccgc acgaatgttt ttccaagcgc cgtttcgatt cactatattt
    22801 tatcaaaaga aaaccaatat attagcagag atattttctc tgctgaatga gaacgagcta
    22861 gcgatcgcag atatatcgcg taaccataag aatgagcatg tctacatatt gattgacata
    22921 gacagtactg attttgaaca gctcaatcaa gtgaaacaac agctagaaaa aatttctgga
    22981 gtacgaaagg tccgcttatt aaagaaacca gaaaggccat aaagaaagag aatatttgga
    23041 tttcattaat tcttctttcc gttctttact ttcgaatttc gtacaactaa aataagagaa
    23101 gcaacattct tttattgata gaaatgaagg agcaagcatt tgaaaagata tataagcaga
    23161 ctttcgacca aacagaaaaa gattttaaaa ggaacagcag caatcatcag tatctttttt
    23221 cttgttgttt ttagtaattt atttttgcag tggtgtcaaa atgatctatc cgtagacctt
    23281 gcgttgaaat ttgctttttc ttggcatacg gaaaaattct tcttagcctg tctcgtcttg
    23341 ctgattattc ttatttttct gattgcacta gcaggatctg tccctttagg ttcccttacg
    23401 tatgtcgtag ccatcggtgt attaggtttt gcaaattata tgaaaatgag ttatcggcaa
    23461 gaaccgattt atccagacga tttgaagatg atcactgaaa tcggattgct gaaagatatg
    23521 accggcacta tgctgtttac agtgatctta gctgccgcag gaaccgtgat aggattgttt
    23581 tgctggtaca tgtttcgaag cctgaaaaaa ggaaggcgat tccaattgat ccgattaacg
    23641 acactgcttg tggcaattgg actgctaggg tatatcagta atttcaacaa tcccgataat
    23701 ttgttgcgaa aagcatataa taaaacggca ctatggatac cttacagcca gaaaatgaac
    23761 tactataaca ctggttttat cggtgggttt ttgtacaatc tgaaggtaga accaatggat
    23821 gaacctgaag gatattcgaa agaaaagatc aaagaaatca ctgaaaaata ccagaagcta
    23881 gccgatgaaa aaaataaaac tgtagaggaa gagtcgccga acatcgtgtt cgtgatgagt
    23941 gaaagtttct cagatcctag tcgattaaac ggcgtagaag tatctggtga gccattggca
    24001 gattattatg aagtagctga tcaaacatat agtggcaata tgctttctca aaactatggc
    24061 ggtggaacag caaatatcga atttgaagca ttgactggtt tttctatggc gttatttaat
    24121 gcgcaactca ccacccctta cacgatgctt gtaccaaaaa tggatcagct tccttctatc
    24181 gtttcagcgt taaacgcaca atcataccag acgacagcga tccatccata caatacttcg
    24241 atgtataaaa gagaggatgt ttatcagaca ttaggatttg accagttcat cagcgagcgt
    24301 acgatgacct atacggatac gatagagaat aatccatata tttctgatga gtcagcttac
    24361 aaagaagtaa tgacacttct caaggaagaa aaaacacctc agttcattca tttagtcacg
    24421 atgcagacac atatgcccta cgatggaaag tatgataatc tagactatcc tgcaaaaact
    24481 tcagatggaa gtgggacact tgacttagag aattatctgc aagatatttc ctatagcagc
    24541 acggcactga agcagttcac ggaagaattg aagaatcttt ctagaaggac tttagttgtt
    24601 ttttgggggg atcatcttcc agggatctat tcagatacga tccaagcaaa aaatgacaaa
    24661 caaacattgc atgaaacaca atttcttatg ttcgacagta aaggagaatt agaaaagcaa
    24721 acgactcacg atgcaatcac cagtccgttt tattttgcag cgaatctaat ggaacagacg
    24781 aatcagacaa caaatggctt ttatcagctg cttttatcat tagaacagga acttccggcg
    24841 tttgaacgag aactgtacta tcaaaacgga cagtggtata aagaagcgca attcaatcgc
    24901 tcgcaacaag agatttacga cgaatatcaa ttgatccaat atgacattgt cgcagggaaa
    24961 caatatagtt tagcagatgg tttctttgag cacgaataag aatggcgagc tgtatagaaa
    25021 ccattaaatt ttttcaagta cttgtcattt gaggataaaa acaagtagaa taaataataa
    25081 caacaatgga gaaagtagga aaacgaacat gttaccgaat attggctatt atgtgcagtc
    25141 catcgatcaa atcgtcaagg aaacagaagc aatcggagaa ggaatgcatc cgaaatatga
    25201 agtgatcaga aacgcaattg atgaaggaaa aacaagtgaa ctggacaaat cgactcttga
    25261 agagatcatc ctgttatttg aagaaggaac aaaaaaatat cgggcaatgc ttatgaaagt
    25321 cagtactttg cgtccaccag caaaagcaat gggcattcat aaaaattttg aaaaagctta
    25381 tctttcatat gtagcaggct gtgaagaaat gatccaaagt atcgatcttg aatcaggggt
    25441 agatgaagat ctattcaata catctgaagc aaaacaagac caagcgacag accaattgac
    25501 agctgccatc caaaaaatga ctaatttgtt gttgaaaaaa taaaagaaat ctttgataac
    25561 aataaaaact agagttatct aatataaatg taatattacc tttatgatag agtgatcaaa
    25621 atcctttatg ataatgacat acctataacc aacaacaacc ttgtacttac tcaaaatcga
    25681 gtaaatactc cttttccgtc gagtctaacc atctcggcgg ttttttattt gtagaaaaga
    25741 acatcaggat agacggaaaa gtcttgtcct gatgttcttt tcagatagta tggaaagctg
    25801 attagttttc tacattctgt acatattgtt caaagtagtt ctgtaaatcg gctttgagtt
    25861 tctgatattc ctcttcgcta tactctttat cttcgatttt cccatcaaag aaaggaatat
    25921 ttagttcttc ccgtagtctg tctaatacgt cttgctgatt catttcattc tccttttctt
    25981 actaacgatc aaaataaaca cagacacatt ctggtacata gacatctttt ttcagtaatt
    26041 ccagtaatcc tgtttcagga tgacggcgat ataatgtcag attatcggtg ttctgattgg
    26101 cacacacgac aaaattattg cttggatcta gtgcgaaatc gcggggaaaa tccccttctg
    26161 ttgagatgat ttgcttgttt tgaatgtggg aaccatcttc aaaaactgcg aatacagcaa
    26221 tcgagttatg tcctcggtta gaagcataaa ggaacttgcc atcatttgaa atgcggatag
    26281 ctgcccctcc attttcaccg tcgaattctt ctggcaaggt cgatattttc tgtaattctt
    26341 caaaagcacc tgtttcttta ttataagaaa gcacgcggac tgtgctgtct aattcaccaa
    26401 ataaataagc gaattgtcca tttggatgaa agacaagatg gcgagggcct gttcctggct
    26461 ctgcagtgaa ggtagttaca agattcaatt tgccgttttc ggacacatca taagtataca
    26521 cacgatctgt accaagatca catacgacta atcgttggtc aggagtcaga tccgtatagt
    26581 ggacatgcgc atgatcttgg tttttatgag gacctgtcgc ttcttgatgg aatattgaat
    26641 ctgctgcttt cagttcgcca tctcctaata tatggtagac atttacttca cctttatgat
    26701 agttcgcccc gtatagtaat tgacgtgctt catcaaccgc tacataacac aaaggagcgc
    26761 cagactctgt taccgcattc aaaaaatgaa aatccttatc gtacgcaccg gcaccaccta
    26821 atccatccac actagtaact gtgtaaagat tgccagcttt actttttgct acataggtag
    26881 ggctattttc ttcaacagct aatgaaaggt tggctaattc ttctttttct gtatcaaggt
    26941 cgatcgtata gattccttta ctttcgcgac gcgtataggt tcctaaaatt atcttttcga
    27001 tcatatgagc tgattcctcc taattgtaat gaaatagttt tcgtttttat tctatcacat
    27061 aattattcca cacggctaaa gaatgagaat gtgttatact gtttttgaaa gcaattaaag
    27121 gagagagtgc aatgattcag gcaaagatta cagaaattgg gacagaagca ataaacaagg
    27181 aagaacccat gctgattttg ttcgataaaa cagcaacagc tgccttacga aaatattcgg
    27241 tgattcaaga agtatcaaaa gacgaacctt tttctttgaa agcaggcgga acaattgctt
    27301 ttgatgatca ggtatatacg atcgattatg tcggtccgat ggccaatggg aatttaaaga
    27361 cagtagctca cgtatcattg atttttgatg cttgtccaga agaaggacaa attgcaaatg
    27421 gaatctatct tcatccgcat agactgcctg ctattggttt gggaagccaa attttttata
    27481 actaacagga ggaattatgg aaggaaaaaa taagaagaac agaccggtat cgtggttttg
    27541 gcgctggttt ttaaataatc aagtagtcac agccttactt gttgtattgt taatattgct
    27601 gatcgtcttt ttatttacaa aagtttcata tttgtttgca ccaatctggc aatttttagc
    27661 aatcgtaggt ctgcctatca ttttagcagg aatcttgtat tacttgatga atccggtagt
    27721 tgattacttt gaaaagcgaa aagtaccaag gctttacagt atcattgggc tgtttgtttt
    27781 ggttgttgca ctaatcgcat gggggagcgt agtgatcatc cctaagattc aggaacaaac
    27841 agtgagcttc atcagtaact tccctcaata tgtagatacg attgataata aattaaccga
    27901 gattttgcgg gacccgctgt tcaaccaatt tcgcgaacag ttggaaacag caggtgataa
    27961 gttcatgaat tcggcaggcg atatgatcca agatatatct aagagtacag ttcagagtct
    28021 tggaagtttt gtcggcgcgg tagcgactat tttagtggct ctgctgacga tgccgtttat
    28081 cttattttat ctattgaaag acggtaaaca attagcacct tattttgtca aatttttacc
    28141 gacacgcatg caaaaaccga cgttgaatgt gctgagtgaa atgaatagtc aagtttcttc
    28201 ttatataaga ggacaactaa ctgtggcttt tgctgtggca atcatgttta tgattggttt
    28261 tgcggtcatt ggtttagagt atgctgtcac gttggggatt attgcgggtt ttttaaacct
    28321 gatcccttac ttaggatcat ttttagcgat gattccggct atctttttag ccattgtagc
    28381 aggtccattc atgatcatca aagtactgat cgtttttgca gtagagcaga caatagaagg
    28441 acgcttgatc tctccattag tactagggaa tcagttagca atccatccag taacgatttt
    28501 attagttctg ctgacttctg ggaaattgtt cggaatcatc ggtgtgatct tggggattcc
    28561 tgtttatgca gcagctaaag tagtgattac tcatgttttt gaatggtaca caacaatctc
    28621 ttctctttat gaagaaaatc cgttgaaaaa gcagaaagaa taacaaaaag agacagatgg
    28681 gaaaatatcc aaggcacaaa tgctgctttg aagatttttt cctctgtctc ttttgtatgc
    28741 atttatctca atttttaaaa tgatttacct aaagcagcag ctttatctaa tgcattttgc
    28801 atcaactcat ctgcacggtc agggtcataa tcgattcctt cgatgaatag ctgatctact
    28861 tgatcgatgc ctacaaagtt taaaatacct tttacatatt gagaggcaaa gtcttgtccg
    28921 ttgtatacac cgccattaga ttggatgtgc agtgcttttt tacctgtcac taatccttct
    28981 ggcccgtttt ctgtatagcg gaacgttttt ccagctacat tgacggtatc gaaccaagct
    29041 tttaattttg ttgggatatt tagattccat aaggcgtttg cgataacgat tttatcagca
    29101 gcaaggaact gatcagtcaa ttcattgaaa cgagcaattt tttgttgttg actttctgat
    29161 aaggaagtga attctgcacc agcacgtaat gcttcccaac ctgataataa ttcttcatcg
    29221 atttccggaa tatcatcgcg ataaagttcc aaaacagtca cttcatcatc tggatgattt
    29281 tctttataac ttgttaaaaa agacgtcaat gccttaactg tgcgcgattc ttctgcagtt
    29341 aggggatgac ctttgactac taaaacagat gccatatgct taccttctcc ttttattgct
    29401 atgattgtta taactatgac taacaaaatc agtttactcg aaaaagaaaa ggctatcaag
    29461 tcatattgat aaggttgaag aataaataac taaaaaaaga cagggtataa aagaggctgt
    29521 gacacgattt tttgtcacag cctcttttga atctaacaag caatattcaa gcaacactcg
    29581 actgaagagc ttccatccca ccaatgatat aaacagcacg aaaaccatta gcagtaagtt
    29641 gatcggctaa tgcttctgat cgcctgcctg tatagcttag aatataataa agtttctttg
    29701 gatctagatt ttttaattgt cttggcaaga tagcagcggg gatagaaagc gagtggctta
    29761 agtggttttt tgtgtaatct aaggtatttc ttgtatctaa aacgatgctg tcagcatcga
    29821 gaggcaattt attgaagtct tggacactaa ttgattgggc catttatctc atcctttgtt
    29881 tagtatgtgg ctattttaat gggcaactct cattaattca aatggaagga ttcctcttat
    29941 cactttttta atgtatcgga tgataatgaa aaaaactatg caaatgtctt gcaaaacagg
    30001 taggaggtgt tataatgaca tagcgtcata tgacaatgtg tcatcttgaa agaaaggaga
    30061 atccatatgc cgacacagac atttttccat ttgcctaaag aaaaacaaaa aagactaatc
    30121 gaggctgcga gaatcgagtt ttctagagta cccttaaaag aagcttcgat tgctaatatc
    30181 gtcaaattag cggatatccc tagaggcagt ttctatcagt attttgaaga taaagaggat
    30241 ctttattttt attactttga gacaatgaga cgtgacagct ctaaagatat gttacaacta
    30301 atgaaagaat ccgatggtga tctattcaaa ggattcgaat cctatttttc taaaatgatt
    30361 tatgagattt tggaaggcga gaatgcttct ttttatcgta acttgtttat gaacatggat
    30421 taccgtgcct caagaaaagt cgctcctcat acgagtcgcc aaaaaacgcc tcacgatcca
    30481 cataaagaac acaaacaaga tgcaaaagag ttgcttgaat tgatcgatcg gacgaagctg
    30541 aaagtaaaaa atgatcatga attggaatta ctgatccaat tagtgatgaa tactgtattt
    30601 acaacaattg cgcattcgta tcgcttattt tcaacatctg aagattactc agtagagcaa
    30661 gcaatcagtg aattcaattt gaaactttct tggctgaaaa acggagtata taaatagaaa
    30721 gaagggaact agatgctaaa aatcgtaaaa cggatctcct tgacttcagc aattgcagct
    30781 gcagttttca tggtgatcca agtactggca gatttatatt tgccgacact tacctccaat
    30841 attattgata aaggggtagc gcaaggagat gtcgattata tttggcatac aggttttgtg
    30901 atgatcggct tttctttgat cagtatctta gctgctatcg caaatacatt ttttgctact
    30961 cgtgaatcac agaaactggg taagcaattg cgtacggatg tctataaaaa atcagagagt
    31021 ctgacaaaag atgcgtttga taaatatgga acggcctctt tgatcacaag aacgaccaat
    31081 gatgtgactc aaatccaaat ggttacacaa atgtttttac gtatgatgat caatgcacca
    31141 atcacgttga ttggcgcgag catacttgct tatcaaaaag atcatcagtt gacaaaaatc
    31201 ttcttagttg tcatcccagt aatgattatt ttgatcggcg gaatcatgta ctttgctgtt
    31261 ccattattca aaagtatgca aaagaaaact gaccgtttaa acctggtatt tcgagaagga
    31321 ttgacagggg tcagagtgat tcgagcattc gataagacac gctttgaaga aaatcgtttt
    31381 gatttagcga ataaagatta tacaaatacg gcaatcaaag ttaatacgat cgtcgcattg
    31441 atgatgccaa tgatgacatt gatcatgagc ggtacaaacg tagcaatcac ttggtttggt
    31501 ggacactaca tagcagatat gactttggaa gtcggtaatc tgattgcatt tatgacttat
    31561 gcgatgcaga tcttgatcag ctttatgatg ctttccgcta tttttatcat ggtgcctcgt
    31621 gcacaagctt cagctgaccg tattaatgaa gtgttggatg aaaaaatcgg tatccatgac
    31681 cctgaaaatc ctaaaacagt ttcttttgct ggaaaaaacg caacattggc cttcaatcat
    31741 gtcaattatc gatatcatgg agcagaaaaa cttgcattgg aagatattga tttccaagcc
    31801 aagtcgggcg agatcgtcgc aatcataggg gggactggtt caggtaaaac gaccttagtc
    31861 aatttgatcc ctcgattata tgacatcgaa tctggttcga tccaaataaa tggaacggac
    31921 attagtgaca tgactcaata taatttgcga gaattaatgg gctttgttcc tcaaaaagca
    31981 gttctgttct ctggaacgat ccgagacaat atgcagtatg gtaagccgga tgcaacagat
    32041 gaaatgattt ggaaagcatt agaaatcgca caagctaaag attttgtttc agagatggaa
    32101 gatggcttag atagccatgt ggaacaagga ggaggaaact tctctggtgg acaaagacag
    32161 cgtctggcaa ttgcacgggc attagtcaaa acagcagata tctatgtgtt tgatgattcc
    32221 ttctcagcgt tggatttcaa aacggatgcc aatcttcgcc aagcattgaa aacgaatatg
    32281 aaagaaagta tcaccgtttt agttgctcaa cgagtaagta cagtaatgga tgcagatatg
    32341 atccttgtgc tagatgaagg aaaactggtt ggcaaaggaa cacatgaaga actattagca
    32401 acaaacgaaa cttatcaaga aatcgttcat tcacaactaa gagaggagga cttagcatga
    32461 gtaaaacaaa cacacgtccc tctcatggac cagcaggcgg tccaggacat ggtggacctg
    32521 gcagaaatct cggtgtaaaa ggtgaaaagc ctaaaaattt ctggggaacg gtacgccgat
    32581 tgtttggcta tatgtctaaa cgtatggttg cgatcatcgc tgtatttgta ctagcaatag
    32641 cagccgtgat cttccaaatc caaacaccaa aggtcttggg gaaagcaacc acagaaatat
    32701 tcaaaggtgt aatggctggc gctcaacaga tgcaacaagg ccaacaagta acaaaatttc
    32761 cgatagactt tgataaaatc gcgcagatca ttgcgacagt aatcgtgatg tatttgattt
    32821 ctgctgtatt caacttttta cagcaattta ttatgacacg tgtttcccaa cgaacagttt
    32881 atgaattacg tcatgatttg gaagccaaaa tgaatcgtgt accgatttcc tattatgata
    32941 cacatacaaa tggggatatc atgtctcgag caatcaatga tatggataac atcgcaagca
    33001 cgctgcagca aaatttgact caatttgtaa ctagtgcagt cacattgatt ggggtcatcg
    33061 tgatgatgct ttcaatcagc tggcaattga cgttagtcgc gttattgatg atccctttaa
    33121 gcttgatcat cgtgatgatc gtagcgccga aatcacaagt gtttttcgca gatcagcaaa
    33181 aaagtttagg tttattgaat aaccaagtag aagaaacata tggtggtcat accattgtta
    33241 aaacattcaa tcatactgaa aaagaccagg aagtatttga aaaagaaaat cagaatctgt
    33301 atgccgctgg ttggaaagct caatttattt cggcaatcat catgccgttg atgaatttcg
    33361 ttaaaaatct aggctatgtg ttcgtcgctg tactaggcgg agtaaaagtt gcaaatggga
    33421 acatgacatt aggggatgtt caagcgttct tgcaatatac aacacagttc tctcaaccaa
    33481 tcactcaact tgcaagtttg atgaacacta tccaatcaac agttgcttca gccgaacggg
    33541 tatttgaagt gttggatgaa gaagagatgt ccaatgaaaa atctggcctt ccagaagaaa
    33601 aagatacacc atacaaagtt cgttttgaaa atgttcaatt cggctacaca ccagataact
    33661 tgctaatgac tgattttaac ttggatgtca aacctggtga gatggtcgct attgtaggtc
    33721 cgactggtgc agggaagacg acactgatca accttcttga acggttctac gatgtttctg
    33781 gaggtagtat tcgttacgat ggtgtagata cccgtgatct ttcacgtgat gaattgcgcg
    33841 cacagttttc aatggtactg caagatactt ggctgtttac aggaagtatc tatgacaata
    33901 tcaaatatgg gaacgacgat gcaacagacg aacaaatcat tgaggctgct aaagctgctc
    33961 acgtagacga ttttgttcgt aaattgccag aaggatataa cactgtatta aacgaggatg
    34021 caagcaatat ttcgcaaggg cagcgtcagc taatcacaat tgcgcgggca ttcttagcaa
    34081 atccagatgt cctgatcctt gatgaagcaa catctagtgt agatacacga acagaaatac
    34141 tgattcaaaa agcgatgaac cgcctattgg aaaaccggac aagtttcgtt gttgctcacc
    34201 gcttgtctac aatacgcgat gcagacaaca ttattgtgat gaatcatggt tcgatcgtag
    34261 aaacaggaaa ccacgataca ttgatggaaa aagacggatt ctatgcagat ctttataaca
    34321 gtcagttttc tgaagaagaa gcttcataga atagtacaaa tttattattt tttttagggg
    34381 ctgtgacaaa agtcgtgtca cagtcccttt ttccgaataa acggtgttca atcagctgtc
    34441 cttcggtatt agctcaaaaa acggcaaaat atgagaagct atttttccgt tttgtatctc
    34501 taatactcag gtcaaaccgc tgattttaca acctcttcct taaacggtgt tctcctgcca
    34561 actcctcggt cacaagtcac aatagtcaac aaacctgaga agctgttttt tgaaattgct
    34621 ccttgtgact cggagttaaa cggcaggctc acaacctctt ggtaaacggt gttcaaaagc
    34681 cagtttctcg gtattaagcc aaatttatgc aaaaatgtag aaagccattt tcacaaaatt
    34741 tttcttacta ctcgaaacta agcgactttt tcacaacctc ttccttaaac ggtgttcaaa
    34801 agcttgcatc tgcggtaata agcccaacca aacgaaaaat aggaggagct atttttgttt
    34861 ggctgttctt attacttgat gcagaacagc tttttcacaa cctcttttta taagagtttt
    34921 tactcattaa tttacaaaaa acaagcaaaa tgcgaatttt ctcacatttt ttttaacaaa
    34981 atcaaagtta ttgcttgaaa gaagaagaat ccatcgttaa aataaagctt gacgtagagg
    35041 ggagcaagaa atatgaaaga ttttcaattg ggggctgcca cctatttaaa aagtgtagcc
    35101 attcgggtga aagaccgaga taaaatgatt gatttttaca agaacgttgt tggctttgat
    35161 ttgaaaagag aagaaaacga attagccatt ttaggaacag aagaagctgg aagtgaaatg
    35221 ttgctgctgg aagaaagtcc tagagcgaat gatcacatgg gggtgatcaa aaagctgaac
    35281 cgtttttcat tgattatccc tagcacagaa gaaatggcag atatcctttg tcgtatccgt
    35341 aaaatgaatt atccaatcaa agaagcttta gaagatcatg gacgtatggg gattttgctc
    35401 gtagacccag aagagaatga attggaaatt taccatgaag agaataagaa cgaccaaaga
    35461 gaaattccag aaacaatgga tcaagaagca ttattaagaa aatctgaaga aaaattcagt
    35521 aaactatcaa caggttctta ttttgaaaaa atccatttaa atgttactaa cttaacaaaa
    35581 gaacgccaat ttcttaatca tgtacttggt ttgaaggtac ataatgaaga agagggcatc
    35641 catgtattga atgaaggaga tttccatgtt ggtttgacag aagctagcgg aggcgcaatt
    35701 gatttgccta cagatgaagt gattggactt gattttctga aattcatgat cgatactgaa
    35761 agcatggaag gactgattgc tcacttgcag caactatcag ttgatttctt cttagacaaa
    35821 aagcacacag tactcacaat ctatgatgct ataggaatcg aatggtggtt cgtcagtgag
    35881 ccaaaagaaa agaataaaac tgacattcga tgcgacgctt aactttttct ttcttaacga
    35941 ttgtcgcatg tctcgtcatt ttatgaacgg tcatactatc gcttattcta gagatagaaa
    36001 gagaaaatag tataccgggg gaaactgcgc ggtttttcgc gcagtttttt tgtgtaaaaa
    36061 aggttgattc ctcatagatt aagctttaag cttggactgt aagaatctga tagaaaagca
    36121 ggagaaaaag atgtatccac acacgaaaca gcttattttg aaaaagatgg aagaaggatt
    36181 ttatccaggt gtagcagtcg ccttcataaa agaaaatcaa gtagaaacat ttacagccgg
    36241 ctttgcccaa gtcgttcctg aaaagcagct gatgacagaa gatctgttgt ttgatgcagc
    36301 ctcactgacg aaagtcgttg caaccacttc attagtttta cgtctattag aagatggaaa
    36361 gatagaactc gatcaaccgc ttcataaata tcttcctgaa tttgaaaatc agacaatcac
    36421 tttgcgtcat ttactgacac atacttctga tatcaaaaca tggataccaa acagagataa
    36481 gctatctcag gaagaattga tcgccgctta tttgaagcta gaaccaggtg agcaactagg
    36541 aaaagaagta aaatatacag atgccggtac gattcttctt gggttgatgc tagaggaaat
    36601 ttaccaagaa actgcagtgg aaatctttca agaacaagta ttggaaccat taggaatgat
    36661 gaacagctta tttcttcctt ttccttccaa aaaaatcgtt cccacagaac aactgccttc
    36721 gggtgaggtt ttgcgtggac aaactcatga tccgaaagca agagttttgg ctgaacatgc
    36781 aggtaacgcg ggtctcttta ttgatttgaa agacagcatt acttttgtcc aaatgtattt
    36841 gaacaaggga aaaatccgta atggtcgttt tttagaagaa gagacgattg attctttact
    36901 gcaagatcaa acaccgaaaa aaaatggcta tcgttcttta ggatgggact taaaaaagga
    36961 tgtagaagac ggtcatgcga ttctttttca tactggttat acaggtacgt ttttcttgat
    37021 tgatataaaa acacaagaag cgttgatatt tttgtcaaat cgcgtacatc ctgtcgatca
    37081 tcgtgcggag tatctcatat gtcgagatga aattgttgaa aactttttga aagagaaagc
    37141 ggtattaccg aaataatgct ataatagcta tgaaaaatga gaggagtttg aattatgcag
    37201 gaacctatgt ttttaaaacc agtttttcaa gaaaaaattt ggggaggaag tcgtttacgt
    37261 tcagtctttg gttttgatat ccctaacgat aaaatcggag aagattgggc gatcagtgcg
    37321 catcctcatg gtgtcagtgt agtagaaaat ggcgaattca aagggaaaag attagatgaa
    37381 ctatggtcag aacacaagga attgtttggc catccttcag aaccagtgtt tccattattg
    37441 atcaagatct tagacgcaga agatgatcta tctgtacaag tacatccaga tgatgcctat
    37501 gggatgaaac atgaaggtga attaggaaaa actgaatgct ggtatatcat cgatgctgaa
    37561 cctggtgcag aaatcatcta tggtcatcat gcaaagacaa gagaagaact agctgagatg
    37621 atcaaagatg ggcgctggga tgaccttttg aagaaagtac cagttaaaaa aggtgatttc
    37681 ttttatgtac caagcggaac gatccatgct attggtaaag gaatcatgat tttagaaaca
    37741 cagcaaagtt ctgacacgac ttaccgcgtg tatgactacg acagaaaaga tgcgcaagga
    37801 caaacaagag agctgcatat ccaacaatcg atcgatgtaa caactgtccc tgcgaaaaca
    37861 ccagaattac aaatcaaaga agtccgaaaa gggaattcat cgatcgtcac ttatttagaa
    37921 actgagtttt tcaatgtata tgagtgggat attaaaggaa tcacatcttt caaaaaacaa
    37981 gccccttata cattgatgac ggtaatcgaa ggagcaggag accttgtcgt tgacggaaaa
    38041 acatatccat tagaaaaagg aacaagctgt attattccaa atggcgtttc tgagtgggcg
    38101 gtccaaggtg aactagcaat cattgcgtct gaaccaggca aatagttccc agatagctga
    38161 aagcagcaga cggtttttcg tttgctgctt tttctacata aaatccgtcc atcaataaat
    38221 agaagtctgg tgaaaaagat ggaatacaaa tggaaagcaa acaacagcag tcgcgtgtct
    38281 atgaaacaac tgagaaaagc tggacatcac ttaaatgaaa tgatcatgcc aaatctaagt
    38341 ctgtttatcg cttggggaat cttgtcatta gcaacaaaag gtgtgtctgg cgatttgcac
    38401 accacattag aagaggtatg ccattggatg atccagctgc ttttacccat cttgatcagt
    38461 tattcaggcg ggaaatcggt agggggacaa aaaggggcag tcgccggtgc aattgcttct
    38521 ttagggttga tcgctcgttc caatgctcca caaatcgtag gagcgatgat tatcgggccg
    38581 cttgccggca tcgtatatga acaatttgtt tctagattcc atgtgaaatt caaggctgga
    38641 tatgaaatgc ttgtcagcaa cctactagta ggactgactg gtagcgttat ttgtataatt
    38701 ggaatcgttt ttattgaacc agttttaaat agtgttcact tgttgctcgc ctccgttgtg
    38761 tcttggctag tcacaatgaa tctgttacca cttgtcagtc tgatcttaga acctctaaaa
    38821 gtactatttt tcaataatgc aatcaatcat ggtgtattga cacctttagg aatcgaattc
    38881 tcacaaacag tcgggtactc ttccttattt ttgatggagg caaatcctgg acctggttta
    38941 gggatcttac tttctatcct ttgttttgca gaaaaacagg aaaaagtaaa tgcgagtgga
    39001 gcattgatga tccatgcgat tggcgggatt cacgaaatct attttccatt tgtcttgctg
    39061 cgaccgtatc tatttttagc tgtaatggct ggtggggcaa gcggcacgtt gatatttcaa
    39121 tggttagata cgggattagc tgcacctgta tcgcctgggt cactactcgt gatttttgcc
    39181 aatgcgcctt atccacaact agctggggta agtataggga tagcaatcag tacatttgtg
    39241 accttcattt gtgcagcgat actattgaaa ttaccaaata aagaaaaaga gaacaaaacg
    39301 gtcaaagaaa ggggagaaat cttggaacca tctatcattc attccgtcat ttttgcttgc
    39361 gactcaggta tgggatcaag tgctatggga gccgccctgc tgaaaaagaa attagcagca
    39421 gaaaaaatcg atctttctgt cggatacagc tctatctatg aattgaataa ccaagtaaat
    39481 cagctggtta tcgtgcaaca tgaactagcg ggtcttgcaa gagaaaaagt acctcaaagt
    39541 caaatctatg tactggatca ctttttggaa acagatatct tgctgtcacg aataaaagaa
    39601 gcgatccaat ttccaaagcg ggaagaaaaa aaagagatag cagatatcac agaacaaaaa
    39661 acgtcgcctg ctaatattgt atttttgtat aaaaacaaca gacgcggctc acaaactatg
    39721 ggcatgacga ttttaagtca attagcaaaa aaagcgaaaa aacagtttgt tatctcaaaa
    39781 gaatcaatag aaaaaatcaa aatccaagaa gaaacgattt atatcgcaca aagagaacta
    39841 gctgtaactg aacagctaga agaaaagatc cctcggctag ttctagttga acattgggta
    39901 gctacgaatg aatacgaaaa actcgtgaag ggagaaatat aagatgtatt tatcgcccag
    39961 agaaaaacaa ttattagcag aatttctcag tagcccgtca cctgtatcta tccaaaaaat
    40021 gatgaattta ttgaaggtca gtaaacggac tatttaccgc gaattagata atcttgaagt
    40081 ctcattgaaa tcggtaaatg cttctttgaa aaaagcagct agaggctcat ttgtcattga
    40141 ggcatcagaa gaagtattag agcaattaaa agaaaataca gatgggaaaa gtaatgactt
    40201 atccactgct aaacgacaac acgcgatttt agccgagttg ttactgacga cagagccgtt
    40261 gagtcttgct catttcatgg aatcatatcg catcagtaat accacttttt atgcagatat
    40321 caaacagttg gaagaaagta ttcgccaact gcctttggaa atcattcgga accagggata
    40381 cgagatcgaa ggaccggaaa aataccgccg gcttctgaca gcgaatgtat tagaattaga
    40441 aatcaatgaa tatgaacttt ttcatagtat ttctttcgat gcatcactga attatttttt
    40501 tcgattcgtt gatccgcaac atctatctct tgcacgcaaa gtaatcgggg aagaattgat
    40561 ccaaaaaaag accaatctaa gtgatagaaa gcttgaacat ttggttttga tgctgacatt
    40621 gacgatggag cgtgtgacga aaaatcattt actgacaaat gagacatata atagtctggc
    40681 aaacaaagaa ttactacaga cagctaaacg attgttttca cggatcgctt ctgagacaaa
    40741 acaactttat ccagtaaatg agatcgtctt ttttgcagga ttattaaatg acttcacgaa
    40801 ctcatttgat cagaatttct ttgaagagac gttcgataca cagcttgctt atttagtaaa
    40861 acaactgatt caagaagtaa gtgaagagac gaatgtccat ttttatgaag atgaaacttt
    40921 gtacaaaatg ctgttgactc atttatctgg tgtgttttca agagccgttt tacaagaaga
    40981 gacactgacc aatccgattt tagaaaaaat catggttcaa tacgaggaag ttgcgaatgc
    41041 aatccgtaaa gcagtagagc ggatttttca agaaaaaaaa ttatccgaag aagagatcgc
    41101 ttatatggtc cttcattttg cgaattcatt ggaacgaagt ccgaaagaaa gcaatatcag
    41161 tattgcagga ttttcgccaa gtggattagc ttctactagc atgttggaac tacgattgaa
    41221 aaggtatttt cccttcatcc aacaaattca tttctatcgg attgctgatt taaaaaaaat
    41281 cgatttacaa gaagaatatg atgttgttat ttctacatct attttaccag gatacggtgg
    41341 aaaatatatt ttagtctccc cgctactgct agaagatgaa gtcaagcagc taaaagaagc
    41401 tttttcgcat attgagaaga aaaaaaggca aaaaattacg tataacagca ataacgagtg
    41461 gatgatagaa gatacttatg aggaaacaat gcatttcatg gatcaaatca atcaattact
    41521 cggtcgtttt tttatccaag aactggataa tcctgagacg atttccgaaa cgattcgcag
    41581 cttatttcct tattttcctg aaggactagt aacggatgaa gaggtggttc atcgccggct
    41641 gcttaagcga tatgaacaag cacctatcgg tattcctcat acaaatatgg ggttattcca
    41701 tacatccagc tcattgattt ctaaaccgat cttttgtatt ttcaatttga aacatccgtt
    41761 gaccattgaa gggatggata gacagccagt cgatttgtcc cgtatgttag ttatgctggc
    41821 gccttcgcca atccaagaaa aagacgggat tcttttagga aagatcagtg gagcaatcat
    41881 catgaatgac ttgaacaccg agatcttccg ctcaggaaat aaagaaatcg tttatcagtt
    41941 gttagcgaaa atcctgattg aagaaataaa gaaatgaagg tgaaaagatg atacagctac
    42001 cagcagaaaa aatattgctc gatcaatcct atcaaaattg gaaagaagcg ttgaccgaga
    42061 tcggccagtg gatgacccaa caagggggaa tcaaaccggc atatatccaa tcaatgatcg
    42121 aaagacaaga aaaagcgagt gtctatatcg ggaatttcgt agccttgcca catgggactg
    42181 acaaagatga agaagtagta gaagaaggaa tctatttagt ccaagtgcct gatggtgtgg
    42241 actttggaac aaaagaggaa ccaaaaatcg cgacactctt atttgtagtg gctttaaaaa
    42301 aagaacatca gttagcggtg ctccaagatt tagccttttt ctgttcagac atcgaccaag
    42361 taatggcgct cagtgatgcg gaaaccattg aagaagtcca aatgattttt gaacatggac
    42421 atatataacc aatgttcaca gtaaaaaaag ctgccttgca tcaagtaata acttcaacca
    42481 agataaaaat aagaggagct atttttgttg ggaggttctt attactgcgg atgcaagctt
    42541 ttgaaaacta tttattctag aaaaccgttt atttgaaata ggggacggtg acaaggtttt
    42601 tgtcaccgcc cctattattt ttgtgtttta atagctcttt tttatataga tagtgcccat
    42661 acctatgaat aataaaacga tactccatat accaagtgtc aagataccat tcatgaaagt
    42721 aacctgttct gaagctagtg gcgagaaaat atctcctcct atgatcacat tttgaaaatc
    42781 aacataagaa gttggcaagt aatgactgat tgattctatc catccaatcg aagaaaacaa
    42841 gcgatactct gctaagaaaa gacaaagaag tgtacctaaa agattgacta gagaatgttc
    42901 aaataaagca gatagaagaa aactgattgt acttaaaaag agcacccaca acaacaggaa
    42961 cagaaagctc tggaaaagat actcatacat cggcaggatt cgtatctcct gaccaatgat
    43021 cgttcctaca ggataacgga agtctcccaa accatttttg attattacta gtatagagac
    43081 gatcaaaaat ggcatcacga gacttagcat agctgcacca taataaatgg taaaacggat
    43141 gctatgtttc tttaccatac tatccggtaa tagattgact agagagatta cctgctttct
    43201 tttttcataa gagatgatgg aagaaagaaa caaggcggtt atacataaaa tcaaaaaact
    43261 agaaatcatt cctgaaaaaa tattttcgta gtaatttgct aaagagagat gcgctggcaa
    43321 gtcaaaaata gaatatcttt ggatctgatg ttctttcata taacggagca actcgacacg
    43381 tttttgttgt tcaatgattg gaattccttg tctttgacca gaaatcagtc gatccagtat
    43441 atctttttca taatgataag ttgcttcgac catttgctgg acattccctg tgttttttgc
    43501 atgtaagatt gcttcgatca gtttgttgct tttctctaaa ctttccacaa attctttctc
    43561 attctcatct tctttcaacg tttcaatcaa acgttgcatc tcttcatagt tatgattcag
    43621 ttcatcgaat tctgtataag caagtttatg atcaaaaaaa agtgggaatg caaagagcac
    43681 gaccaacaat agaggaaata aaaagtttac tttgtttttt agtaagagat tgcgttcaaa
    43741 tgtaagtaaa gacatagtta cacctcatat aatttacgat aaatagctaa aatctcttcc
    43801 atatttgagg attctgtgac gatttctcca ttttttagaa aacatgcttt agaacaaatc
    43861 gattggatat tcgcgatagc gtgggaagaa agaagaatcg tcttcccctc tttctgccat
    43921 tgtctgatga ggctattcaa gatatcaata cttcctgggt ctaatccgtt caacggttca
    43981 tcgaataaca tgacagatga atcactgatc aagtaagcag caagcaagac atgttgtttc
    44041 attcctagag acatttttcc aatcggcaaa gtaaggtact cagtcatctt caatcgttgg
    44101 aaaacagttt ggcattctac tttgctctgc caatagtatt tgatcaaagt caaataatca
    44161 acagccgata gatagtcgga taactgattc gttgattcta gaaaaaagaa agaccggcga
    44221 taatctgtat aagcagtagg agagatgttg tgaagggaca attgatcata agttgttggc
    44281 agcagttggg caatgccatt cagcaaagtt gttttgccaa atccgttcgg ggcaatcaaa
    44341 gcagctactt ctcctgattc tactaaaaga ttcaattgtc gaatcacacg ttttttacca
    44401 cgtttcatgt tcatgttttt gatcagtaat gtcatagata ggcctccaat ataaaattta
    44461 ataacataat agaatggaaa acatctaaat taaatgctaa caaaagttat tgtttgcata
    44521 ttataaaaaa attaaaataa cagttttatt taaaattgaa gttttctgat accgaaatct
    44581 ttttattttg ctgacatact caaacctttg gcacaaagtt tcgtgccaaa gtacgactat
    44641 ttctcttttt agtaagcgga tacaattaga gtatcaaaag gagagaggga gagacaaaat
    44701 ggaacggacg attcccaaaa acaacatttc gctaaaagcg agggtacaaa aattaggcag
    44761 tactttatca agcatggtca tgccaaatat tggagcactg attgcctggg gtgtattaac
    44821 ggctctattt atcccagatg gttatttacc aaatgaggct ttcgctacca tggtaagccc
    44881 aatgctaact tatctgattc ctttattgat cggttatact ggcggaaaag taattgctgg
    44941 tgaccgcgga tcagttgttg gggcaattgc aacgatgggt gtgattgtcg gaacagatat
    45001 tccaatgatg ctaggcgcga tgatcatggg acctcttggt ggatatgcca tcaagaaatt
    45061 tgatcagcta ttccaaaaac ggatcaaatc cggttttgaa atgctagtca ataatttttc
    45121 agcaggtctt atcggttttg gattagcgtt attaggtttt tctgcgattg gtccagttgt
    45181 cgatgcattg actcaagcaa tggcaaaagg tgtagagatc attttatctg cccatttgat
    45241 tccattgaca agtattttta ttgaaccagc aaaaatctta tttttgaata atgcaatcaa
    45301 tcatgggatt ttgacaccat tgggaactga acaagtagca gaggctggaa aatctattct
    45361 attcttgctt gaagcaaatc ctggtccagg acttggcgta ttacttgctt ttactttgtt
    45421 tggcaaagga gcagcaaaat cttcagcacc aggagccatg atcattcact ttttaggcgg
    45481 gatccatgaa atctattttc cttacgttat gatgaaaccg ttattattct tatcagttat
    45541 aggcggagga atgtcaggaa gtttcatttt ccaattgatg gatgctggac taagagcgcc
    45601 agcttctcca gggtcgatca ttgcgattct agcgatgaca ccgttcaatt cttaccttcc
    45661 tgttattttg ggcgtgattg ttgccgcagc tgtttcgttt ggtatttctg cagtaatttt
    45721 gaaagcggat gcgaaagata caaatgaaga cttcgaaaaa tctgttcaag agacaaaggc
    45781 agctaagcaa gcttccaaag gtgcccaagg tattcctcaa ggagctacat tctctggcgt
    45841 acaaaaaatc atttttgctt gtgatgcagg aatgggatca agtgcgatgg gcgcttcgat
    45901 cttgagaaaa aaagtacagg aagcagactt gcctcaaacg gtgaccaatc aagcaatcag
    45961 ccaactgaca gatgactcga atacattgat cgttactcag gtcgaactac aagaaagagc
    46021 aaaacaaaaa gcaccgagcg cgcgttttgt ctcagtagaa aactttttga attcacctcg
    46081 atatgatgaa atcattcaag aattaagcag caagcagaaa acatcagttt ctgaaattga
    46141 gaatgagcca gcagaagagt ttctttcagc aaacatcaaa caaatcaaag agattctact
    46201 tgtccacgat gatcgttccg gatcagcaac aatggggatg accgtgctca aagatatatt
    46261 agacaagaac catttaacta tcccgttaag aaaagtcaat atccaagatt taaaagatga
    46321 acctgattca ctgatcgtag ccaaagaaag tttagcggaa aaagtacaga tggctgcacc
    46381 tcaatccatt catttatcag tcaacagcat ggtcaatccg caaaaatacg aatcgattgc
    46441 acaatatttg aaacagacag cttaagggag gaaaacagca tgcaaagttt gccattcgac
    46501 atgatcgagc tcaatcgatc ttttaaaacc aaagaagaag cgatccgcta ttgcggtcag
    46561 atgcttgtag aagcagggtg cgttgatgag gaatatatcg atgccatggt agatagagac
    46621 aaaatgttat ctgtttatat ggggaatttt atcgcgattc ctcatggaac agatgaagca
    46681 aaaaaatatg tgaaaaaatc cggcatctgt gtcgttcaag taccagatgg tgtagatttt
    46741 ggtaccgaag aagaagaaaa gattgcgact gttttgttcg gtattgcagg agtcggagat
    46801 gagcatttgc aacttgtaca gcaaatcgca ctttattgca gtgatatgga caatgttgtc
    46861 cagctagcag acgcgctcag caaagaagaa gtaacggaaa atctagcaat tgcttaagga
    46921 gagagaaaaa tgagagcagt acattttggt gcaggaaata tcggtcgagg atttattgga
    46981 gaggttttgg cagctaatgg atttgagatt actttcgtag atgtgaatca cacgatcatt
    47041 gatgctttaa atgaaagaaa agaatacgaa atcgaattag cagatgaatc aaaagaaaaa
    47101 atcactgtaa aaaatgtcaa aggaatcaac aatcaaacaa atccagaaaa agtaatccaa
    47161 gaaatcgctg aagcagattt agttactacc gccattggcc cgaatatctt accttttatt
    47221 gctgagttga ttgcaaaagg tatccaagaa agagaaaagg aaggaagcac aacatcttta
    47281 gatatcattg cctgtgaaaa tatgattggc ggttcagcat ttctagaaaa agaagtgtat
    47341 aaatatcttc cggaaacatc atttactgac aaatatatcg gctttccaaa tgcagcagtc
    47401 gatcgaatcg tcccattaca acaccatgaa gatcctttat tcgtccaagt ggaacctttc
    47461 aaagaatggg tcattgacga tagccaaaga aaaaataaaa aaatccaatt aaaaggggtt
    47521 ttatatgtag atgatctaga accatatatc gaacgcaaac tatttagtgt caatactggt
    47581 catgcgactg tcgcttatac aggtgccttg ttaggttacc agaccattga tgaagcaatg
    47641 caagatgcgt tagttgtggc acaattaaaa tcagttttgc aggaaacagg aagtttgctg
    47701 attgccaaat gggggtttga tgcagagcag catcatgcgt atattgaaaa aatcattcat
    47761 cgcttccaaa acaaaaatat ctctgatgcg atcacacgtg tcgctcggac accattacgt
    47821 aagttaggtt atcaagaaag atttacacgt ccagtgagag aactacaaga acataacttg
    47881 acttgcccac atttgacggc aacaatgggg attattttta attactatga tcctgaagat
    47941 gaacaaagcc gtcaattgca tgaaatgaag atccatgaaa acttggaaca attgattcaa
    48001 gaagtcacag gaatcaatga tccgaaaaca atcgggaata tcaagcaaaa cgtgaaccgt
    48061 tatgccaagc aagtcgctta aaataggata cgaaaaaggg gaatgtgacg aaatttttgt
    48121 cacattcccc tttttcgaat aactaggctg tttgtccttc aagatagggc agatccaatt
    48181 ctttaccgta acgttgttct aaagctaggc ggatcaaacg agctgccagg ttctcatttc
    48241 ttgcaaggat aggtccatgg aaataagaac cgaagacatt tcggtaaatg acaccttcag
    48301 attggtcttc accattattt ccttgacctt gtaccacttt ccccaaaggt cgttcacctt
    48361 cacctaagaa agtccgacca ttgtggtttt caaatccata ataagtttca ttgaattctt
    48421 cgttatggat aacgatatca ccgataaacc ggttattttc ctgacttaac gtgtagtgat
    48481 ccagtgcact gattccttgg atcttttctc cattagctcc catgtagtag tgacctagca
    48541 attggtagcc accacaaatg gctaacatga cgccatcatt ttcgatatag ttgatcaaag
    48601 aatctttctt tttctggatg tcttttgaaa tgattaattg ctcaaagtct tgaccaccgc
    48661 caaagaaaac caagtcatat ttatcagcat caaaaggttc atgtatacta acgatctccg
    48721 tatggcaaga aacccccatt ttttctgcta tatatttcaa catcaaaagg tttccgttat
    48781 caccatatgt gttcatcaaa tttccataaa ggtgggcgat attgagttca taattagcca
    48841 cggttcattc ctcctttgat aaatccttga gcagctagtt ctttacgaag ctgcaataca
    48901 gcagtgtaag tagctaaaat atagacatgc tcagttggca attctttgat tttttcgatt
    48961 acttctggta attcttttgt ttctgtcaat ttattttctg gaatcccggc aacttttaga
    49021 cgtaaagtca tatctgtatg acggtctcct ccagaaataa cagctgggat atccatatcg
    49081 gcaaagtttt caaaattacc atcccaaatc cagctgacat cgattccgtc tgcgtaattg
    49141 gcatttaata aagccactaa agaaaatgga taaggagcta gaccaatcat atcgattact
    49201 tgattgatac ctacaggatt tttgacaagt actaatgtac attttttccc attgatattg
    49261 atgacttctt gtcggccgaa tactttctcg tcatatccta aaccttgttt gattttatca
    49321 ggatcgacat tgaagtactc tgccactgca gtagctgcta aagcattgta gacattatac
    49381 ataccgccaa cttcgattcc gtattcatga ccatcgatca cgaaatctgc tgacacattg
    49441 tccatccgta ccatatcagt taatcgataa tccaattcag gacgtttgaa gtcacaattt
    49501 ggacagtaat atttccctaa gtttgcataa gtgatcattt tataatgcaa gatgtgatga
    49561 caatttggac ataagacacc gtctgtattg taatgagcca tttgttcacg atcttcttca
    49621 tggtcaaagc cataatattt tctaggattg actgtttcga tagaattgaa gattggtgaa
    49681 tcaccgttac tgataatcgt tgcatttggt gactttgcgg caccgtctac aatcatttta
    49741 taggtcgtat agatttctcc ataccgatcc atttgatcac ggaaaatatt tgtaaataag
    49801 aacagttctg gtttcatata ttctgtgact ttgctcaaac ttgcttcatc tatttctagg
    49861 acagcaaact ttttctgtcc tttttttggt tttgcctgca agaaagtcga tacaattccc
    49921 tgtaccatat tcgcaccagt agggttggtt agaacttcat cgaattgctg gcgcaaaata
    49981 ttgactgtta aagcggtagt caatgtttta ccgtttgttc cggtgacaac gacgacttgg
    50041 tagtcttttg ccaaactatc tagtatatgt ggatcgactt tcagcgctag tttacctggt
    50101 aaacttgatc cgcctttaaa aaaagttttt aatgcccatt gcgacgtttt tccaactgca
    50161 atcgcaaaat gactgcgtat tcccattatg taacctccaa aaacgatttg ctgacgaata
    50221 aattcaacta atcataccat atgttgaaaa aaaggttaaa ttgcttttct tattcttaag
    50281 aaaaggttac ggctgaaagt gcatgaaatc ggacaaaatc gcatgattta acaaaaaaac
    50341 agcttgaaac aaaatgattg tttcaagctg ctgtgatttt attattccct cttacctata
    50401 ttcccatttt aaccgataat aattttctcg cttggatatt catagcctag atctcgtgtt
    50461 tcttttggca cgaataacat caatgtatat agcattccga tccgcccaat aaacatcagt
    50521 gtagcaatcg tgattttgcc cacaacagac aagtctccgg taatccctaa agataaacca
    50581 gttgttccaa acgcagaagt tacttcgatg atgatcgaga tcagcgtttg ttcttctgta
    50641 gcagataaaa aaacgataca aaagaaacac ataccaagag aaagcataaa gacaacgact
    50701 gattttcgca catcatcttg atcgattctc cgtccaaaaa tattgatgtt atcttcactt
    50761 ttgaggaagg aatatagata caaaccgatg attgctacag tagttgttcg aatcccgccg
    50821 cctacagaac taggactgca tccgataaac atcagtaaag aaaaaatgat cagtgttgtg
    50881 atttgaaaat cacccaaatc gtgaatctgc agaccagcat tacgagtcgt aatcgaataa
    50941 aacatcgaat tgatccattt gacactcata ttggaatctt ggaataaatg gtccttttcc
    51001 aatagataaa tcaagacagt tcccgaaaca aacaagataa caaaagctaa cacagctaat
    51061 ttggtaaaca aggaaaaacg aaacggaagc ttcgcattgg aacgtttgta taatagccat
    51121 tcccggcact ccatcaatac aggaaaacca attcctccga caaaaatcaa aaacatgatc
    51181 aggatcaaga aaaaatagtc atgtgcaaag ggcttgatgg aatccccagt gacgtcaaac
    51241 ccagagttcg tgacagccga tatcgcctga taaaaaccgt aaaaaacggc atcacgccaa
    51301 cgatcaaaat agcctctgta ataaaaataa atagaaaaga atgtcccaaa aagtaattga
    51361 aaccagatta aaatagcaaa agtgatccga atcagtcgga cgataccaga caaacgcggt
    51421 tgattcatat ccgtcatgat cagctgtcgc tgtttcagtg tgatccgtcg tttagaaaaa
    51481 atgacaaaag cagtagaaat catcatgatc cccaaaccac caacttgaaa taatatttcc
    51541 agcagtataa taccgttatc attgaacaca gaattgatat caaaagtcga tagtccagtc
    51601 acactcactg tactgattgc catgaacagc atgtccacaa aagaaacatg gctatctggt
    51661 tcgcgaaaaa tcggcatata aaatagaacg agtgataaga ccgtcatcaa tatataataa
    51721 aaaacgatga tttggatcga tgagaaatga ttggcagcaa acttctgtcc tcgccgctgc
    51781 agccttcgaa agaataaatc aacatgcaca gtactatcct cctgacaata gtcgtttcta
    51841 ccagtataca aggggaatta ggaatactca aagaaattat caaacagata acaatacagc
    51901 tttcgattaa aatagaaatg gcttataata aggaaaatcc tagaaaaaag aaggaggagg
    51961 aataacaaat tgattaatgc tcaacaaatc gtagaaaaaa tgaaaaatca aaaaataaat
    52021 tatgatacag ttctgaaaaa aatgatcatg caatgggaga aagaagaagt aaagcctaag
    52081 atattaatac atacatgctg tgctccttgc agtacgcaca cattggaatt catgtctcaa
    52141 tatgcagaga tcacgttatt tttttcaaat tcgaatattc atcctaaaag tgagtatttg
    52201 cgaagattac atgaacaaaa acgatttgta gaccaattta atgagaaaac tgaccatcat
    52261 gttgcgctaa tcgttgatga ataccgtcca aatgaattca ccaaaatggt tttaacaaac
    52321 catcttgaaa acgaaaaaga aggtggtgct cgctgcagtg catgcttcaa tatgcgatta
    52381 gatcttgttg cgcaaaaagc gcaagaacta ggatacgatt actttggaag tgccctgacc
    52441 atctcgccaa agaaaaatgc agctttgatc aatcagatcg gaatggatat ccaaaagatt
    52501 tacgggacac aatacttgcc aagtgatttc aagaaaaaca aaggctatga acgctctttg
    52561 caaatgtgca aagagtatga tatttatcgc caatgttatt gtggttgcgt gttcgctgcc
    52621 aagcagcaag gatgcgatct gaaagaaatc aatcgggaag caaaagctta cttaaaaaca
    52681 tcaaccgtag tttttgagga aaattaaaga aatctttcgg aaaaacgtaa gaaaaaaata
    52741 aagaatttat ggtagaaata aggtagactg tataatttgt ccgtagtaaa gtaaggaaac
    52801 aagaataaat ttcaatgaag gaagagataa gatgcgaaaa gtagcaccta ttttgaagcc
    52861 aatcgctgta ttaacaatct cgacgattgt cgcgactact acaggtattg tgaatagttc
    52921 cagctaccgt gcaccaagtt caagttctag tactgtagaa gaaacaacac caactacttc
    52981 agtctcgaca tctgaaccaa caacaacgga aagtttgaat gaaaccacta cgacaagtac
    53041 atgggacgat acaacaacta gttcgtcgta tgatgagaca tatacaactg acagctcaag
    53101 cgaaacaagt aatacggtac cagatggcac tccatcggat tcaaccaatc caagtacaag
    53161 tccggataca agtgctagct ccgaaacgat accaagcagt acgactgtag atgataacac
    53221 gcaaaatgat gatgcagcac gtacagcaca gcaaaaccaa gattcaggaa atacccaaac
    53281 gtataaccag caagcaggtg attctcaatg acatttttac tagaccttat caacggggtg
    53341 cataaattcc ttggctattt agacatcagt cctaaatatc ttaaccgtgg atatacgatt
    53401 ttaagtgtga ttcccacgct ttatttattg agaattgttt atggactttg gcaaaaccaa
    53461 aactatcttc aatttttctt atatggactt gcatttctag tgcttgttta ttttactgtt
    53521 ttaaatgtat tttattactt tttagataaa aacacgaaag ccgatgtcac tcagcttttt
    53581 gtgaagtatt tacctgatga agcgttcaac atccagacag aagaatcgat agataatgga
    53641 aattcgatcg ataaagtgaa tacacaagag gtcatggttt cttatgacga agattatcaa
    53701 ttgaaattag ccgagaatat gcggtatttg attggaaatg gtgagatcaa aacaaatgaa
    53761 ctcggtcgca tggatggatt tctggtcgat agaaatacat tgtatcctta ttactatgtc
    53821 aagaaaacag gagagaaaga gtatgttcta aatattggac gaagctactc ggacatggaa
    53881 aaagtaggga ccattcgact tgactcaaca gaagacacat tggaacctgt cggattgttc
    53941 atcgtcggcg gtgattttgt caaagacggg ttacgctatc atgagcctta tcgtctgaaa
    54001 ttgattgcaa aaaaggcaca gtcgcaggaa gaactagtgg tttatagtcg tagccaaaga
    54061 agaagacatt cataatgtga tgaaacgccg tgtatcagtg gttgcgataa aaacgtttat
    54121 tcggaataag ggggatgtga cacgtctttt gtcacgttcc ttttttatat aacgagaaag
    54181 ataagacgaa cacatcactt attctttcct ttcatttata aacattgtag aataaagaaa
    54241 gtacaacgaa gtattgtgaa aaaacgtaat aaataacgag gaaaatacaa gtaggaggta
    54301 agctgtgaaa gaatttttga aaaattgggg gattttgatc cttgtattag cggctatttt
    54361 attagctaga gtgtacgtgt ttacaccagt tactgtaaac gggcattcga tggaccctac
    54421 attaagtgat ggacaacgat tgatttcatc taagatctca aactatgaac gaatggatat
    54481 tattactaca aaggaaccgg gggatgaaga acggatgatt gttaaacgga tcatcggtat
    54541 gcctggagat acagtcaaga tggaaaatga ccagttgacc attaatggca aaaagtatga
    54601 tgaaccttat ctggatgagt tcaaaaaaga attttcagaa gataagttac aaggagaata
    54661 tgcttatagc tcaggattcc aagcacaagc agaaagttca tccaccttta cgagtgactt
    54721 tgaatatact gtgccaaaag gaaagtatct tgttctaggc gataaccgct tgatttcaaa
    54781 agacagccga atgtttgggt tagtcgacaa agatatgatc caaggaaaag ttgttttccg
    54841 ttattggcct ctttcagaaa ttaaaatcat gtgatgaaaa aataaaaaga agctgtgaca
    54901 tgttttttgt cacagcttct ttttccgaaa attccttctt atttcttgga gttgctcact
    54961 ttttttgcaa tctcatttta agctttttgg ctgatggata agaaaagcga agcgtttttg
    55021 ttcataaata gcttttcccc acgatggaag gtatgactag tagcagagac aaagcccgtt
    55081 ttttccaatc gattattcaa ttcttcttgg ctaaagccat tatggacttt cggatgacta
    55141 atttgttcat tcttgtcaaa gtctacgatg atcaattgac cgccaggagc taaaatttca
    55201 taaagttttg taagaatatt ctccgtatca ggaatgtgta aaagtacaag tgatagaagg
    55261 atgagattag cttggacagc aggaaattcg acagaagcat cagcgtggat cgttcggaca
    55321 tttttcagat ctgctgcttg gatcttttct tctgccattt tcagcatagt ttctgaagca
    55381 tcggcaatga tcagttcttt gaatctttcc gcaagcggta aactaacaag cccagttccg
    55441 cctccgtaat ccagcaggac tttttggtgg ctgtcgcgcg gtagataagt agttagttct
    55501 tcgcgaataa tcttggctaa tgcttgacgg tcaggggaat cataatggtt agcgatggaa
    55561 tcaaaaatat tttttgtcat caaatagctc cttacgtaaa actttgaaat tttaacttat
    55621 tatagcacgt gaaaagaaag aaagcatgct atactcaatt caaaaaggga gaatctagaa
    55681 agaatatgcc gatatcgttt gtaaaagata gagaggaaaa aggaaagtgt gtccgagaaa
    55741 tattactaga cttgcctgag tggtttgggc ttcctgaatc aacggaaaaa tatatcgagg
    55801 aatcaagtaa actaccttta tggtgtgaaa aaagaaaaga agaatattta ggctttatca
    55861 ctctttcaca gacaagtgaa gatactgccg aaatctattc tattgtatgg gaataaaaaa
    55921 agactatcat caccaaggat taggaacaaa actttttaaa gaagcagaag gttacgcagc
    55981 aaaacattac aaatatttgc aagtgaagac tgtagacgag gggcactact ctatatatga
    56041 tcaaactatt tgtttctacg aatcattagg attttcaagg ttggaagttt ttccaaattt
    56101 atgggatgaa tggaacccat gtttagttct tgtcaaaaaa ttggaacaaa agtgagcaat
    56161 agatttaata ctaatctgtg cttgatgaaa attctcactg taaaaaatgc tgaaaatcaa
    56221 gtataatacc ataggaacaa actggaaggt gaaaaagagc atggcacaac tattttttaa
    56281 gtatggcgca atgaatagcg gcaagacgat cgagatccta aaagtcgcac ataattatga
    56341 agaacaaaat aagccagttg tcttaatgac aagcggtatt gatacaagag atggtttagg
    56401 cgttgtttca agccgaatcg gactgaaacg taatgcgatg ccgatatttg aagaaacaga
    56461 cgtatttgac gtaattcaga aaatggaaag caaaccctat tgtgtgctga tcgatgaagc
    56521 gcaattctta aaaaagaaac atgtcattgc ttttacgaga attgtggacg aattggatat
    56581 tccggttatg gcatttggtt tgaaaaatga ttttcgaaac gagttatttg aaggatcaaa
    56641 atatttattg ctttatgcag acaaaatcga agaaatgaaa acaatctgtt ggttctgcca
    56701 caaaaaagca atcatgaatt tacattatat tgatggaaac cctgtttatg aaggcgatca
    56761 agtgcaaatt ggcggaaatg aagcttatta ccccgtttgt cgaaaacatt actttcaccc
    56821 atcaatgatt acagaaggag aataagcatg tacgatcaat tacaagcaat tgaagaccgt
    56881 tatgaagagc ttggagaact tttaagtgac ccagaagtga tttctgatac aaagcgtttt
    56941 atgcagcttt caaaagaaga agcaaacacg cgcgaaacag tcgaagttta tcgtcgttat
    57001 aaagaagtag tacaaggaat caccgataca gaagaactgc ttggtgaaaa actggatgat
    57061 gaaatggctg aactagcaaa agaagaatta tctgaattaa aaaaagaaaa agaagtctta
    57121 gaagaaaaaa tcaagatctt gcttttacca aaagatccaa atgacgataa aaatatcatc
    57181 atggaaatcc gtggagcagc aggcggagac gaagcagcat tattcgctgg cgatcttttc
    57241 aatatgtacc aaaagtatgc tgaagcacaa ggctggaaaa cagaagtgat ggaagctaac
    57301 gttaccggaa tcggtggata taaagaagtg atcatgatga tcaccggaga caacgtatat
    57361 tcgaaattaa aatatgaaag tggtgcgcac cgcgtccagc gtgttccttc aactgaatcg
    57421 caagggcgta tccatacttc caccgcaaca gttgtcgtga tgccagaagc tgaagaagta
    57481 gagatcgata ttgaagacaa agatatccgt acagatatct atcatgcttc aggtgctggc
    57541 ggacagcacg tcaataaaac agcatctgct gtacgtttga ctcacttacc aacagggatc
    57601 gttgttgcta tgcaagatga acgttctcaa atcaagaacc gtgaaaaagc aatgaaaatc
    57661 ttgcgtgctc gtgtgtacga taagatccag caagaagcac aaagcgagta tgatgccaat
    57721 cgaaaatcag cagtcggaac tggggatcgt tcagaacgga tccggacgta taatttcccg
    57781 caaaatcgtg tgactgatca tcgtatcgga ttaactatcc aaaaattaga tcagatttta
    57841 gcagggaaat tagatgagat cgttgatgca ttgatcatgt atgatcaaac atcaaaactg
    57901 gaagctatgc aaaatgggta aaatgaccta tcgagaagtc ctgtcacggg cttcttcttt
    57961 tttagaggat catgggaaag aagcatatag tatccaattt ttatttttgg aacgaaaaca
    58021 atggaaaaaa cttgattggc tccttcatat gaacgaagag atttcagaag aagaacagcg
    58081 gttgatcgaa accgatctgc gacttctgct tgcagatcat ccacctcagt atttgctagg
    58141 atatgcagat ttttatgatc atcggttaaa ggtaactgaa gcaacactga tccctcgacc
    58201 agaaacagag gagttagtcg aatggtgttt agacgagacg cctggtgtac cgttagaagt
    58261 gatagatatt gggacaggga ctggagcaat tgctattagt ttgaaagcag caagaaaaaa
    58321 ttggcatata tctgctgtgg atctttcaga agaagcatta gaagtagcga aagaaaatgc
    58381 tcagaaagaa ggaacgaaaa tcagttttta tcatggagat acattaggac cagtaatgga
    58441 tcagcaattt gatgtgatta tttctaatcc gccgtacatc agtcggaatg aatgggaact
    58501 gatggatgaa agtgtccggt cattcgaacc taaaatggcc ctttttgctg agaacgatgg
    58561 attagctatt tatgaaaaaa tagcagttga agcaccaagc gtactaaaat caaatggaaa
    58621 gattttttta gaaatcggat ttcgccaagg tgaagcagta aaaaaaatat ttcagcaagc
    58681 atttccagat aaaaaagtgg cgataaaaaa ggatcttttt ggcaatgaac gaatgatcca
    58741 agtttatggt tgaagttgtg gatgaaaaat tttttatttg tttttttatt ataaaaaaac
    58801 acttatttga caaggatgta aacgactttt tggtttatca acaagtttat ccactattga
    58861 caaatagatt atccacaaac agtggaaaac ttgaggataa actcagacaa aataagaatg
    58921 tatgaaaaag gggttgtgga taaactgtgg aaacgaaacg atatgatgaa acagaattgc
    58981 aagaggcagc cgaagcactg aaagaaggag aacttgttgc ttttcctacc gaaacagtat
    59041 acggattggg cgccaacgct cttttaccaa acgctgtcaa gaaagtgttt tctgtaaaag
    59101 ggagaccgca agataatcca ttaatcgtcc atgtcgcctc ttttgaacaa gtaaaagagt
    59161 atgtggacaa ctttcatccg gcagcagaga agattgtgaa gaacttctgg cctggaccat
    59221 taactttgat atttaagatc aaaaaagaca ctttgccctc agttgtgacg ggaggattat
    59281 ccacagctgc atttcgtata ccggataaca aaaaaacact agaagtaatc gaactttccg
    59341 gtgttccgct tgtcggtcca agtgcaaata cgtcgggaaa accaagtcca acgactgcag
    59401 atcatgtata tcatgatcta caaggaaaaa ttaccgggat tattgatgat ggagctacgc
    59461 gaattggtgt agaatccact gttcttgatt taagtgatcc aacagccatg cctatgattt
    59521 tacgaccagg tgcagtaacg aaagaacaaa tcgaagcagt catcgaatct cctgttgcta
    59581 ttgatcagca tcttgtaaaa gaaaatgaaa caccaaaagc ccctgggatg aaatacaaac
    59641 actactcacc cgatacacgt gttctgatgg tcagagatgg cgattggtca actgcagttc
    59701 aatgggcaaa aaataagaag atccgtgcag gtgtcatagc aagccccgaa attgcggatc
    59761 aagtgcggac agatactgca gctgtttata tgtataacga caactcagta gaagcggcgg
    59821 ctaaaggatt gtttgcaggt ctaagaggat tagatgaacc aacacttgga ttagatttga
    59881 tttttgtaca agtgtatcca gaaactggat tggggaatgc ttacatgaat cgcttgaaaa
    59941 aagcagcagg acaaaattat tttgaaaaat gatcaattgg ttattgtcgc attcttttag
    60001 tatgatacaa tgctatcaag taaatcttct atcagtgaac gaaaaactaa gcgtaactaa
    60061 tcgtaataga agagatacga atatttggca atttgttttt gatgggaaaa ataaaattgt
    60121 aggaaaataa aaaggagctg ttgtttgtgg tagattacaa aacgtttgac cctgatcttt
    60181 gggctgcgat cgccaaagaa gaagaaagac aagaacataa tttggagctg attgcctctg
    60241 aaaactttgt ctccgaagca gtgatggctg cacaaggaag tatcttgaca aataaatacg
    60301 cagaaggtta tccagggcat cgctattatg gtgggtgtga atttgtcgat atcgttgaaa
    60361 atttagcgat tgaccgtgca aaagaattat tcggagcgaa atttgcgaac gtccagcctc
    60421 actccggctc tcaagccaac acggctgctt atctcgcttt ggtagaacca ggggacacga
    60481 tcttaggaat ggatctttcc gcaggcggac atctaacaca tggatcacca gtcaacttca
    60541 gtggcaaaac ctatcacttt gttgcttacg gtgttgatcc gacaacagaa gttattgatt
    60601 ataatgtggt acgtatttta gctagaaaac atcaaccaaa attgatcgta gcaggtgcca
    60661 gtgcttacgg acgaacgatc gactttgcga aattcagaga aatcgcagat gaagtaggcg
    60721 caaaactgat ggtagatatg gcacatatcg caggattagt agcagcagga cttcatccta
    60781 atccagttcc ttatgcagat atcacaacga ctaccactca taaaacattg cgtggccctc
    60841 gaggcggcat gattttgaca aacgatgaag cattagcaaa aaaaataaat agtgcggtat
    60901 ttcctggtat tcaaggcggc cctctagagc atgtgattgc gggtaaagca gtagcattca
    60961 aagaagcatt agatcctgca tttaaagaat atagtgaaca aatcatcgcc aatgcaaaag
    61021 caatggtcaa agttttcaat caagcaattg gaaccagagt catttcaggg gcgacagata
    61081 atcatctgat gttgatcgat gttcgcgagt taggtataaa tggaaaagaa gcagagagca
    61141 ttttggacag tgtgaatatc actgtaaata aaaactcgat tccttttgaa acactaagtc
    61201 cattcaaaac aagcggtatt cgaatcggca cgccagcaat cactactcgc gggttcaaag
    61261 aagaagatgc tgtaaaagtg gctgaactag ttgtcaaagc attacaagca aaagatgata
    61321 acgctcaact agacgaagtc aaaacaggtg tacgcgaatt aactgaaaaa tttccgttgc
    61381 ataaaaaata aaaatttaaa ttccattagt aaacagaaaa gttcaatgtt gggcttttct
    61441 gtttattttt tgggaaaacg gattctttga caatatcggt gatgctctct agtatatgag
    61501 gtgtaatttt agtcttcaca ctaaatctca gggaggaaaa gtcatgactg atcgtatctc
    61561 gttagaaaag gaagcagtag cattcagtga tgcaaatgcg cctcatccgc gtatctatga
    61621 actagcaccg gaagaaggaa gagttttact agaagaagta caagaatcac ctgtgaacaa
    61681 atattccgtc gatattgaag acttgatggt ggatactggc gaatggggaa aactcaatat
    61741 tcgtctcgta cgaccagaag ggaatatgga taaattacca gttatttttt atattcatgg
    61801 agcaggctgg gtttttggca gtgctcaaac ccatgataaa ttgatcagag aacttgcggt
    61861 tcgtacgaat tccgtcgttg tctttccaga atatagtcga tcaccagaag caaaatatcc
    61921 aacagcaatt gaacaaaatt atgctgtgtt gcaacagctg aataaattag ccaagtcaaa
    61981 aaatctggat ctcgagaatc taatcgttgc aggagattca gtaggtggta acatggctac
    62041 agtcatgacg atcttaacaa aagaacgaaa gggcctccca atatccaagc aattgctgta
    62101 ttatccagtc acaaacgctg attttgatac agattcctat catgaattcg gagaaaacta
    62161 ttttttgaca agagaaggaa tgatgtggtt ttgggaccaa tatacgacga atgaagaaga
    62221 gcgtgctgaa atcacagctt cgcctttgag agcaaatata aatgaactaa atgatcttcc
    62281 ttcagcaatg attttaacgg gagaagcgga tgtattaaga gatgaaggtg aggcttatgc
    62341 gcgcaaactt cgtgatgcag gggttcctgt tacacaagta cgcttccaag gaatcatcca
    62401 tgattttgtc atggtaaatg ctatggatca gacaaaggca acgcgtgcag caatggcatt
    62461 atctacttct tggatcaaga aaaaagaata agctttgctt gtccgcagga aacttctttt
    62521 caaaaacgga ttttcttagg aaaaatggcg agtttgacta gtcaatcact tgattttgtt
    62581 ttacaattaa aaagaaacga cagaacaaag gagagcttaa aaaatgggca aatttcaagt
    62641 gatcgatcat ccattgattc aacacaaact aactattatt cgggacaaaa actgcggaac
    62701 aaaggttttt cgtgaagtag tcgatgaaat cgctatgttg atggcatatg aagtatcacg
    62761 ggatatgcca ttggaagaca tcgtgatcga aacaccgatc actgaaacaa cacaaaaaac
    62821 gttgtctggt aaaaaagtag cgattattcc aatcttgcgc gcaggtattg gaatggttga
    62881 cggtatcttg gaattgattc cagctgctaa agtgggacat atcggtcttt atcgagatga
    62941 agaaacattg gaaccacatg aatatttcgt caaacttcca gaagacattg atgcacgtca
    63001 gttgtttgta gtagatccaa tgttggcaac aggcggatca gcaatcatgg caatcgatgc
    63061 attgaaagaa cgtggcgcgt cgaacattaa atttgtttgt ttggtagcag cacctgaggg
    63121 agtaaaagct ttgcaagaag cgcatcctga tattgacatc tatacagctt ctctagatga
    63181 aaaattgaat gaacatggtt atatcgttcc tggactaggg gatgcgggtg accgtttgtt
    63241 tggtacaaaa taagtaaata cgaaaaaggg tgtgacacaa cttttgtcac atcctttttt
    63301 ggtcattttt aacgtttgat gcagaacagc tttttcacag ctttcgtttg catataaaaa
    63361 aaccatccca aaaatgcaga gatggtcaaa ttcaaatctt ttttagttct ttgtagttaa
    63421 cgattactac acccttactt tgcgtcatca agttcgcatt ttctgcagtt tcatcaatct
    63481 caacaacagc ggaatttttc aattgctttg aaatcgttcc agtttgcgac ttgccgtgaa
    63541 ttgtgaacat tacgtgagta ttgatcttga atttttcacc aatttccgat ggtacgatag
    63601 ttgtatcaaa ttttccaaaa gttgccattt tcataaaacc tgcctttcac atcaattata
    63661 ccacgtttta tgaaaaatat catgaaatta aactaatttt ccatcggact ctttacaatc
    63721 agccaaatag agtatataat ttcttttgga taaggccagc ttagctcagt tggtagagca
    63781 acgcactcgt aacgcgtagg tcgtaggttc gatccctata gctggcatta aaagagaaga
    63841 cagatatatc aaggattttt aatccattga tacatctgtc tttttgttta aaggaaataa
    63901 agtatgcttt cttagcaata gagcatgtat tatttctata aaaatttagg agaataaaac
    63961 gactagcaat atagctgtga aatcaaatat cacatgaact gcccaacttg taatgatcga
    64021 gttgctttta atggcagctt ggttgaataa caatcttgcc accccttgta tcaaaaggat
    64081 gtgtgctaca gtgtgtaaaa tatttccatt atcataagta ctgtaatgga taaatccaaa
    64141 gatgattgct gacaagaaac tagcgacagg aatgctgaac ttactagaaa aaatagcgag
    64201 aaaatacact gagaacaatt cttctccaag tatcatgata ggcaaaataa taaataataa
    64261 cgggctgaaa gcctcattta ttggattgcc tgttaaaccc catttcaaaa tttgttgcaa
    64321 aaataccgag actgcggcac tgattactac attgataaaa aaatatttgg ctatattttt
    64381 gacaggagcg acaggtttcg agaacatttt tttcgtatct tttttaccaa aaacaagtgt
    64441 aaatgctata aatccagtga tagttaaaga aaaaatgcta ataatggatg gcgcaaatag
    64501 caaccctaac aaaccgaata caacgacaat agtgcccaat atcccttttt taagttctat
    64561 tttttttcct aaaatgttat tcatgataat aactcctact attcagtttt tgaagataga
    64621 gtcagtatac atgaagagtt aaaataggca atgttttttt atggatattt gtattgctaa
    64681 aaatttatct tatatattca ttttgattat actactttta tttgtaaaaa ttttctagca
    64741 aaaccaagtg cttgcaactg attaagcgta caatttttta gatgttccga gcgtacttca
    64801 atcatttcaa aggagttgct acttaaatcg atatttttta aatcagtatc aaaaaagcta
    64861 gcattttcta aatcattagt agaaaacatt attttagtga gggaacagtc tacaaaatct
    64921 gcgcttgata agacagagtg ctcaaaggta ctttctttta tgtttgaacc agataaattt
    64981 acatagtcca ttttacaatt ctcgaataac gtgttcccaa tataactttc catcaattta
    65041 gtacctgtta atttacaatt gacaaatttc acgcgatgca acatgctttt tgtcaatata
    65101 cagcctgaaa aatcacagtt ttcgaatata cagtcggtaa tctccattct ttgaaaatca
    65161 ttggaagcaa aaaatgcata cttgaaaata gatttgtaaa taattggatg gcagattatt
    65221 tggttttcga tgacctcttt ttgtgaaagg atattataca gttcaaaatc ttcgttttca
    65281 tagatatgaa aaaaggatcc tttattcaag cttgcttcat taactttagg aaaaatcata
    65341 tttaattacc tccattttca taaagtcata ttaacaaaag ctaaaccaac ttacattagc
    65401 agaaaatgag ataggttttc gcctttttcg ataacgatag tgtcgctatc tcttgaaacg
    65461 ttcttagaat tgccctgaga gacacaaatt aggcgtaatt ctaataaaaa tcttagaata
    65521 aaaattttta ctgatttaat ttagaatgtt tttaaaataa aaatcgttgc cataacagtg
    65581 tattgaaagg ttttaacaat ccagtaatga tttaaaaaaa tctagatgta gcgtatactt
    65641 tgtttataga atgtagtggg aagaaatata tttttgggag gaaatcgtga tgtctaaaag
    65701 tgaagaaata gaaaacatgg tactaaaatt atatgatctc gttttaaatc cagagatcaa
    65761 agaaaaggaa cgtatacttt tgatagacgc aaagacaggc ttagaaaaag gtcagtatta
    65821 tcctaaagtc ataaataatt tggaacgtag tttacgtccg ttagcaatca ggggagaatt
    65881 gtctaaacca gtatcaccgt tttatatgga gatatcaaca ataggaaaat ttgaaaaaga
    65941 attaggtcga ggaatggctt cggcaccgat tacttttggg catttataaa aacgagttat
    66001 tcatctcaat gaattaaaaa gtatagtcaa tctcataatg caggctaggt tcatttattt
    66061 gaacagagtc tgtatttttt tattctaaat aaagaaacag atgtttcttt atattgtatt
    66121 ttatccttta attaacacaa aaacagctag tataaactaa taatgttaat taaaagtgta
    66181 ttttatgaaa ctatcgttat ttattaatgt tgcatttttg taagttaaaa attggaaata
    66241 tagattgaaa aaatgaaagc aataagtaaa ataaaatgaa tatatgcgaa aaaataattt
    66301 acattaggaa aaaccttgta aaaacagttt tttggcatat ttatttttta taaatatttg
    66361 taaaataact atcaattttt tagttaaaag attggaaatg agtgggggag gaattgaaga
    66421 aaggattttt gagagatgat atagtaggtt ttatttttag ttaaaatagg ggagatagaa
    66481 aaaatgaaaa agtgtagaaa tattatttta tcaatgttat ttattattac taatataaca
    66541 tcactgattc ctgttcatgt ctatgcggat gcaggcagag atatcagcag taatgtcact
    66601 tcgttgaccg tggacccaac gaatattaca gatggaggaa acataaaagt aaaattctcg
    66661 tttgacgaaa aaaaacaaaa tatccaacca ggtgattatc tttggatcaa ttggccaagt
    66721 gaaggaaata ttcgcggtga aggattccaa aaagaaatcc cattaatgat tgaaaataaa
    66781 aatgttggaa ctttaacggt cagaaaagat tctgcgcagg ttgtgtttaa tgaaaatatt
    66841 aaaaacctag actcagtaga aggttggggg caatttgaga tacaggccag aaacgtaacc
    66901 gataccggtg aagaaaatac gggaccattc accgtgacga gcggtgataa aacagctact
    66961 gtaaatgtga caaagccagc ttctggttct tcttctagcg ttttttatta taaaacagga
    67021 gacatgctac ctgaagatac taagcacatt cgatggtttt tgaatattaa caataacggc
    67081 acttatgttg aacaaccagt taaaatcagt gatgaaatcc aaagtggaca aagattagat
    67141 ccaagtacgt ttgaaattaa tcaaatacac ttaggtgaac agaaggttta tagaggagaa
    67201 gagggaatcc aacaattttt acaagatttc cccagcgcta catttaattt ttcagtaact
    67261 gataattaca ttgaaataac tataccaaaa aactttgtca atcttaggaa aataatggtg
    67321 agttataaga cgatcataga aaacccagaa caaataaatt ttgagaatca ttcggaagct
    67381 tggtttaaag aatttaataa acctgctgtt gatggagaaa gtttcaatca tacagttaaa
    67441 aatatttcag cttctggtgg agtcaacggt acagttagag gcgaattgaa gattttcaaa
    67501 tatatcaatg acacagagat tggtatccca aatgtgactt tcgaattaag acgagcagat
    67561 gaacagccta ttcaaggaca gtctagtatc ctgttgactt cgaatgaaca gggagaagct
    67621 agtatcaaag gcctgcaagt aggagactat gttgtaaaag aaaaagaagc acccaattgg
    67681 attgattttg atccactaag ttcaaatgaa ctcaaattct ccataaacga aaatgataca
    67741 gagggagtca gtttacctat ttataacaag aaaaaggtta cgaacatcac agcaactaaa
    67801 atttggaacg gtggaaccac tccaagaccg agtatttatt ttaaattatt cagagcaagt
    67861 acaaacaatt gggaaccagt tcctgatgca gaaacaaaac gattagacaa tggaataact
    67921 tcagtgactt gggaagatat ccaacagtat gatgatagtg gaaacgaata tacgtttaaa
    67981 gtgcaagaag tagatcaaaa cggaaatgat tatgtccctt caggttatca aaaaattgaa
    68041 aacgggctag tcgttacaaa tgagaataaa gaagtcattt cagtctcagg acaaaaaaca
    68101 tgggaagaca atgagaacca agacggcaaa cgaccgacga cgatcacagt aaatctatta
    68161 gccgacggtc aaccgatcca acacaaagaa gtaagtgaaa aagatgattg gcgctatcgt
    68221 ttcacaaatc tgccaaaata cagagacggg caagaaatca tctatactgt aacggaagac
    68281 aacgtgcccg aatacagtac gacgattgac gggtataaca tcaaaaacag ttatacaccg
    68341 gaaaaaacaa atatatctat accaggtagc catataacac cgtggaaaaa ttttccagag
    68401 aagacagaga gagagaaaat tagaaatcta ttctctcaaa aattgcaaat atttgctcat
    68461 acaggagaat ccagcgaaca aagaagcaat tatattagta agagagctgt acctaaacag
    68521 ataacaaata aatcaaaaag cattcttcct aaaacaagta gtaaaaaatc tagttttgct
    68581 gtaatcattg gtttattgat agtaactatc tgtgctggga tattcttaca aaaatataaa
    68641 aaagtttagt tagaatataa tattttttgt taaatatgac tgaaatcaaa agaataaaat
    68701 tccgatttat aatcgaaaaa agaagcattt ttatatatgc ttcttttttc gtagaatcaa
    68761 taaatatgga tttggctaac tatggaagaa tactcgtatt agttttcagc gttcatacta
    68821 gttgttacat tgtttgattg gccaaaagaa tttctgatat attttgtgct aaataaagaa
    68881 aaagacgctt gaattgggaa ataagaaaac tactctatca ttacaaattt tcataaactg
    68941 gcagatgttc taagtaaaca agtatctttt caaagtcgga aaaatccata atcaacgtag
    69001 tggtattgat atttggatga aagcctacgg tatcttgacg atcaatagca gcatcgatca
    69061 cgacttggat ttttttctct ttgtcgtgca tcaatgctaa tggagtcact gtacctgctg
    69121 gaacgcctag gaggtcagga agctgttctt cagaaacaaa agataagcgc ttttctttta
    69181 attgttcagc tagttgtttt aaatcggcgt gcttttcatc aggcaagatg acaagataga
    69241 tttgttttcc ttttcttgat ttcaataata gattttttac ttgcggacca ggtagggaaa
    69301 aagggacatt tttgactgac gtaatcgcag gatgatcgac tttttgaaaa gagataccca
    69361 gtttttttag tagttcataa gcttcttgtt cggttgcacg actcatcgcg catcaccctc
    69421 ctttttttat tagtgtatca taaaaaagag ttgacaaatg aaaaataatc atgtaaatta
    69481 cacgtaaagt taaaacaaat gacatcgaaa agaagagtag ccgttaggtg tttttagaga
    69541 gtctctggtt ggtggaaaga ggcagcagtt aaccgtgaaa cacatcttgg agtcgatttt
    69601 ctgaagcgta tagtaggaaa atccggggag cccgttacag ctgcggtcgt tgctagatga
    69661 ccagataagg ctggcttttc cgtgaggagt cggtaaaaaa aggtggaacc acgtgattaa
    69721 cgtcctttga tcttcggatc aaaggacttt ttgttttcat ctgaacggca acgactgtgt
    69781 gaggtttttt ctgcaaggaa aaaataaaca gaggtggaac cacgtataat acgtcctctt
    69841 ggcatttgcc aagaggattt ttttatttta aaggaggaaa agaagatgga gtatttgatt
    69901 gaagttatgc cgcaattatt agtaggagca ctcacaacat tgaaattatt tttatttacc
    69961 ttgattggct cattaccatt agggattgtt ttagcattag gattgtcttt gcattttaag
    70021 ccgctacgct acttgcttca aacatatgta tgggttatgc ggggaacacc gctgttgttg
    70081 caactgattt ttatttttta tgggttgccg acgattggga tcgtttttga tcgtttcgaa
    70141 gcagcactca ttgcctttat cttaaactat gcagcttatt ttgcagagat ctttcgtgga
    70201 gggatacaat cgattcctca aggtcaatat gaagcagcgc aagttttgca attaagtccc
    70261 tttcaaacgg tgacgaaaat cgtcatccca caagtgacaa agattgtttt accttctgta
    70321 gggaatgaag ttatcaactt ggtcaaagat tcttccttga tctatatatt aggtttaggt
    70381 gacgtaatgc gtgctgggaa aatcgcgatg gaacgcgacg cgactttact gcctttgcta
    70441 ggcgtggcaa tcatctatct tacattgact ggtattctaa caatcgtaat gaaacaagtt
    70501 gaaggaaaat taaattatta ccgctaactg ataggaggaa gaaatatgat tgaattgaag
    70561 aaagtgaaca aagcatttgg tgaaaaaaaa ataatcaaag atttgaatct aatcattcca
    70621 gatgggaaaa cattagctat cgtaggtcct tccggtggcg gtaaaaccac gcttttacgt
    70681 atcttggctg gcttagaaag ttctgacagc ggagaatttt atttagacgg tgtaccgttc
    70741 aacccattcg agcttaaaat gaaagaacaa gttgtaggag tcgtattcca agacttccag
    70801 ctatttccac atctgaatat ttttgaaaac attaccttag caccaaaaat ggtattgaaa
    70861 cagacaaaag aagaatacac caaaaaagca gaagaattag cggcttcatt agggattagt
    70921 gatttattga atatctatcc ttatcagctt tctggcggac aaaaacagcg ggtagcgatt
    70981 gccagagcat tggctatgaa tccacgggta ttggcttatg atgaaccaac aagtgcttta
    71041 gatcctgaat tacgccagca agtcgagcgt ttgatcctat cattgaaaga agatggggta
    71101 acccaaatta ttgtcaccca tgatttagta tttgcagaaa atgtcggtga tcaattacta
    71161 aaagtttccc caattcatta agaaaggaga agatagagat gaaaaaaacc agttttctat
    71221 tcgctttgat cgcaggtctt cttctttttg caggctgttc gaacaaaaaa acttcagcag
    71281 ataattgggc gactatcaac gaagagaaaa aaatcgttat aggtttagac gatacatttg
    71341 ttcctatggg atttagagat aaagacggga agctcacagg atttgatatt gatttagcca
    71401 aagcagtctt tgaacaatat gggattacgg tagactttca gcccattgac tggtcaatga
    71461 aggaatttga actgaacaat ggaacgatcg atttgatatg gaacggctat tccaaaacgc
    71521 cggcaagaga aaagaaagtg caatttacaa aaccttatat ggaaaacgat caagtgctga
    71581 tcacacctaa agaatcagga atcacaaact ttggacaaat gaataataag agattaggtg
    71641 cgcaaaatgg gtcatctggt tatgatgtat ttacgaaaca accagaagta ttaaaagaga
    71701 tagtcaaaaa ccaagatgct gttttgtatg atagttttaa tgaagcattg atcgatctga
    71761 aaagcagacg ggtcgatggt ctgctgatgg ataaagtata cgcagactat tatttgaaac
    71821 aaagaaacga actagaagac ttcaacataa caaaaggttc ttatgagagt gaggattttg
    71881 cagttggtgc acgcaagtca gatagtacat tagtgaaaaa aatcaatgaa gccctagatc
    71941 aactagagaa aacaggtgaa ttccaaaaaa tatccgataa atggtttggc gaagatgtta
    72001 cgccgagaaa ttaacaaata ggggactgtg acaaaagacg tgtcacagtc cctttttccg
    72061 aataaacagt gttctcctgc caactccttg tgactcggag ctgaatggca ggctcacaac
    72121 ctcttggtaa acggtgttca aaagccagtt tttcggtatt aagccaaatt tatgcaaaaa
    72181 tgtagaaagc cattttcaca aaatttttct tactactcga aactaagcga ctttttcaca
    72241 acctccttta catattttta ttttgttgtg gaaggggttt cgtgttattc taaaagagga
    72301 acgattgaag gttataggaa aggaagagat gaatggaaca caaagaactt acctcatttc
    72361 caaaagattt tctgtggggc tctgcttctg cagcgtatca agtagaagga gcttggcaag
    72421 aagatgggaa aggagaatca gtctgggatc gatttgtacg gatacctgga aagacattta
    72481 aaggaacgaa cggtgatttg gcagtagacc actatcatcg ttataaagaa gatattgctt
    72541 taatgaaaga acaaggtctg aaagcttacc gcttctctgt agcttggaca cggatttttc
    72601 ctaatggacg aggagaagtc aatcaagctg ggctcgcttt ttatgagaga ttgatcgacg
    72661 aattgattga aaacgatatt gaacctatgt tgactttata tcattgggat cttccacaag
    72721 cattgcaaga tgaatataat ggttgggaat cacgtcagat cattgaagat ttcactcatt
    72781 atgcagaaac attatttgag gcattccgcg ggaaagtcca ttactggatt agtttgaatg
    72841 aacaaaatat cttcacttca ttaggttatt tgttagcagc acatccgcca ggtgtgacag
    72901 acccgaaacg aatgtatgaa gttaaccata ttgctaattt agcaaatgca tctgtcatca
    72961 ataaatttca cgagttagaa atgcctggaa aaatcggacc aagctttgcg tatacgccaa
    73021 attatccaat tgacagtaat cctgaaaatg ttctggctgc tgaaaatgca gaagacctga
    73081 tggccaatta ttggatggat gtctatatgt ggggaaaata tccgattgct gccatgaggt
    73141 tcttagaaga aaaaggatgg gcaccaacga ttgaagctgg cgatgcagaa ttgctagaat
    73201 ctgctaaacc agattttctt gggatcaatt actatcaaac ggcgacgaat gcattcaatc
    73261 cattaaacgg agttggtgct ggaaaaatga atacgacagg aaaaaaaggt tcatcagaag
    73321 aaactggagt tcctggaatg tataaaaaag ttgagaatcc atttgtcgaa cgaacaaatt
    73381 gggactggga gattgaccca gaaggattaa ggatcggact gcgtcgtatc acaagccgtt
    73441 accgcatgcc ggtactgatt acggaaaatg gcttaggaga gtatgacaag ttgacagaag
    73501 ataaacaaat ccatgacgac tatcgaatca attacttaca aagccacgtc aaagccatca
    73561 aagaagcaat ttcggatgga gcagaagtcc taggctattg cacctggtct tatacagatt
    73621 tgcttagctg gctgaacggt tatcaaaagc gatatggatt tgtatatgtt gatcaagacg
    73681 aaacacaaga aggatctttg gaacgatata agaaagacag ttattactgg taccaaaaac
    73741 tgattgaaga aaatggagag aacctttagt tcacatactt aaaacaagaa ggtacggctg
    73801 gatatggccg tgccttttct tgataaaatc atttagaaat taagaaaaac tgtaaaataa
    73861 gctgaaaaat gaaagaaagc gtcagttctt tcaaacaaaa ctttagttcc cttacttttt
    73921 tttgtataat gaaacttgat gaaatgaggg ataataaatg gcacaaaaga aacggcctac
    73981 taaaaaaagg aaaaagacga aaaaaacgaa aaagcaaaaa cagcagcagg aacaaatgct
    74041 gacgattggt gttgggtttg ccttgatttt atttgctgtc ttcggctttt taaaattagg
    74101 ttttttagga atattgattg caaacggctt tcggatcatt gcaggaaata cttaccaaat
    74161 cctctgtctt ttattagccg tattaggatt ttggattgtc attaagaata cagagttttc
    74221 aattggtaaa aaccgacgct ggtttggcgg catactcttt tatttcggta tcttattatt
    74281 gttacatgcc catttatttg gcaaactcca tactggggaa ccaaatatca tggggacgac
    74341 atgggatttg ttagccagtg atataaaaca aagtcaagta gacaataacg ttggtggtgg
    74401 gatgattgga gcaatccttt atcattttac ttacttcttg attgcacagc ctggaagtta
    74461 tttagcagca gtgctattac tggcaggagg agcattcctg ttcagtaatc tggaaggcta
    74521 tcagctgtta aatggtatcc aatcaattgg agaacgagta caggaattat tggaaggtga
    74581 tcctgccaag caagctcgaa agcaagcggc aaaggaagag agaatgaaac agcgcgcaga
    74641 agcgaaagaa gcaaggcgct tagctgcaaa agaagctgca gaaaaagaag ctgcagaata
    74701 tgagaagaaa aaagcggtga gccagcaaag ggaaaatact gctgatgaat ggcaggaacc
    74761 agaaaataca gagccggagc agctaagttt tgttccaatc gacagttttc aagaaaatat
    74821 ccatcctgcg aatctagaga aaccagtacc agatacacca aaacagacca atacagcaga
    74881 aggtttttca gatgagttgc cagaggatga cggtacctca ttagaatttg agattgaagc
    74941 agaacaggaa aatcaagact atgaattacc gacagttgat ttgcttgatt ctataccaac
    75001 tgtggatcaa agtgatgagt ataaaaaaat tgaaaaaaat atcggcgtat tagaacaaac
    75061 tttccaaagt ttcggtgtcg atgccaaggt cgtcaaagcc agcttaggac catccgtcac
    75121 aaaattcgaa gtccagccgg ctgtaggggt aaaagtaagt aaaattgtga acttaacaga
    75181 tgatatcgcg cttgctttag cggcgaaaga tgtccggatg gaagcaccga ttccaggtaa
    75241 atcactgatt ggtattgaag taccaaatgg aaagatcagt atggtttcct ttagagaaat
    75301 catcgaagca caaccaaacc acccagataa attactagaa gtacctttag gacgtgatgt
    75361 ttctggacgg gtacaaacgg ctgatctttc gaagatgcct cacttactcg tggctggttc
    75421 gactggtagt gggaagtcag tagcaattaa tgggattatc acaagtatat taatgcgtgc
    75481 taagccccat gaagtaaaac tgatgatgat cgatccgaaa atggttgaat tgaatatgta
    75541 caacggcata cctcatctgt tgacgccggt cgtgacgaac cctagaaaag cagcgcaagc
    75601 cttacaaaaa gtagtacaag aaatggaaga acgatatgaa aaattcgcgg caaccggagt
    75661 aagaaatatt tctggttaca atgaatttgt tcaacagaaa aacttggaaa acggaacgaa
    75721 acatccaacc ttaccattca tcgtagtgat cgtagatgaa ttagctgatt tgatgatggt
    75781 agccagcaat gaagttgaag atgcgattat tcgtttagcg caaatggctc gggcagcggg
    75841 gattcatatg atcctagcta ctcaacgtcc aagtgtggat gttatcacag gaatcatcaa
    75901 agcaaatgta ccttccagaa tggcatttgc ggtatcaagc ggtacagatt ctcggacgat
    75961 cattgattct aatggggcag aaaagctctt agggcgagga gatatgctat tcttgccaat
    76021 gggtgagaat aaaccgatcc gtgtgcaagg agcattcatt tctgaccacg aagtagaacg
    76081 ggtcgttcaa tttgttacag atcagcaaga agctcattat gaagagaaaa tgatgccgac
    76141 agatgaagtt gaaacgacag gtgctccaga acagccacaa gatgagctgt ttgaagaagc
    76201 aaaagcactt gtcgttgaaa tgcaaacggc aagtatttcc cttctccaaa gaagatttcg
    76261 gatcggttac aaccgagcag cacgtttagt agatgaactg gaagctcatg gagttgttgg
    76321 cccgtcagaa ggaagcaagc ctcggaaagt attcattgag caggaagaaa catcagaaac
    76381 gttgccggac gataatgaca tgtaaaactt gtgtgatgaa gggttgaaaa acgaataacc
    76441 aataaaaaga caaggcatga tgataccaaa agaaaaaatg gtttttttcc atcttttcta
    76501 atacggtatg atgtcttgtc tttttagttt ttgtgagatt aaagttctat tcgatttatt
    76561 tgtgaagtat tgcattaagg aaatgaatca tgtatagtat gtagcgaaac tttgagaaaa
    76621 agagggaaaa tactttggaa aaacagtcca tttatggctt gacgaatgaa gaattaatca
    76681 attggtttat agaaaacggc gagaaaaaat tccgagctgc ccaagtttgg gaatggttgt
    76741 atcaaaaacg tgtaagtaat ttcacagaaa tgacgaatct atcgaaacaa ttgatcgaaa
    76801 aattatcgga gcattttatc atcaatcctc taaaacagat ggtcgtccaa gaagcttcgg
    76861 atggtacagt aaaatatttg tttgaactcc ctgataaaaa catgattgag actgtactga
    76921 tgagacaaga atatggcttg tcagtttgtg tgacgacaca agtaggatgt aatatcggct
    76981 gtacgttttg tgcaagcggg ctgctcaaaa agaaccgtga tctgactgct ggagaaattg
    77041 ttgcacagat catgatggtc caacattatt ttgatgagcg caatcttggc gaacgcgttt
    77101 cacatgtcgt tgtaatgggg atcggtgagc catttgataa ttacgacaat gtaatggact
    77161 ttttgcatat catcaatgat gccaaaggat tggcaattgg tgctcggcac attacagttt
    77221 ccactagtgg attagcacac aaaatcaagg aattcgctaa taacggcttg caagtcaatc
    77281 tggctatttc cttgcacgcg ccgaataatg aggtgcgaac atcgatcatg cggatcaacc
    77341 gaagtttccc aattgagaaa ttgatggaag ctgtggatga gtatttagag aaaacaaacc
    77401 gtcgaatcac gtttgaatat atcatgttga atcaagtaaa tgatagacca gagcatgcgc
    77461 aacaattagc agatctatta aaagataaga aaaaattagc atatgtcaac ttgattccat
    77521 ataatccagt cagtgaacat gatcaatatt ctcgcagtcc aaaagctgat gtattgagat
    77581 tttatgatgt attgaagaaa aacggcgtaa actgtgtgat acgtaaagaa catggtacag
    77641 atattgatgc agcttgcgga caattgcgaa gcaagcaaat gaaaaaaaca gttgccaaat
    77701 aatcgtatcc tactcgtttt ttcaattacc ataaaaggag cagttttctt aattaaggaa
    77761 ctgttcaact taaataagag aaaagattct atagcgaaag ctataaagct ttttttgaca
    77821 agaaaaaccc tcatcaagca aagaaatgtc ctcaatttga tgacagcctg cttgatgggg
    77881 gttttagcaa ttatttaact gattttctta tatccttgct ggctttcttg tgtagcatga
    77941 taagtttcta cgtcaagaat ggcctgttta aatttttctc gggtgatttc ataaggcaca
    78001 tgtttaatat catttgttgc catggcttta tctaagattg cttctaaatc ggtcgtgaca
    78061 ttgagattca tgtcggttaa cgatactgga agttggttct ttttcataaa cacatagatt
    78121 ttatcaaaca aatcgaactg ctgatccatc atcaataagg tcagtacacc ataactgaca
    78181 gatgctccgt gaaggtatcc ctccattttt tctagaacag tcgtaccgta atagaatgag
    78241 tgtgccatag cagaattata gttattgtta actaaaacag aaaccaatcc ggtagtaaca
    78301 atgatatccg aaacgacttg ttcaattgca tatgagggac gatcgtcggc actatctttc
    78361 aatgcttgcg taccataagt caagagacga tcaatgcaca tttttccgat ttggattcct
    78421 aatctatccg tgtagcctag atcatcgcca cgagcagaaa aagtagattc gacttctttg
    78481 gataaagcat cgccaatacc tgcccataag tatttatcag gtgcttcggc aataattgct
    78541 gtatcaatga aggtgtgtac aggtggtttt gaaggaaaga agagtccgtc cattacgcct
    78601 tcttcgttat agatgacaca taccgcagtg acgggcgaac agttcgaacc aatagtggga
    78661 aaggtgaaga ctggtttagc tagtttttca ccaatgactt ttacagtatc gcaagcacgg
    78721 ccaccaccga ctgcaaacag gacgtctgct ttttggacac tttcatgctg gatcaactgt
    78781 tcaatatttc cataagttga attacccccg taccagagac tggcgacaac ctctatttcc
    78841 tgtgtcaaag ctacttttat tttgttttct gcctttgaaa gtgcagtctt ccctccaatc
    78901 agcactactt ttgtcccata ttctttgacg attccatttg ctttttcata cgcatctgtt
    78961 ccaatggtat aatttggtaa gaaaacactt tgactcatat tcatttcctt ctttcttcgt
    79021 tgaaacgggt taaaatttta aagagtgatc gatctgactg cggctgtaga tggagtagag
    79081 gtgaacctaa gacaggataa gtgatgaata aggtggcatg aacaccttga aatcatcaat
    79141 aagtatccta aaaacgaaca agtacaaaaa cattgaaaac gacaataatg aatttttgtt
    79201 cctcttagta tttctaccga ttccctttgc ttttttgtaa ttatctaaaa attctaaaaa
    79261 ttaagattat tatatgaaaa caggttttat ttgtcaattc ataattggtt ttttttaaag
    79321 caaaagataa tctatatcat gatgaacaaa cagagttttt tcctccaatt gatggctttc
    79381 ctgatctttc ctattttgaa taaagtgttt ctgtataatt tttttatttt ataagtaaga
    79441 taaataatga aagaatagag atactgattg gtagctaaag atttttgagc gagaacattt
    79501 cgttttttca aaaggactgt tagaataagc aagaacaagg gggaagacgt atgataatca
    79561 gtgaatttga ccgtaataat ccagtattga aagatcagct ttctgattta ctgagactga
    79621 cttggccgga agaatatgga gacagctcgg cagaagaagt agaagaaatg atgaatccag
    79681 aacgaatcgc ggtagcagcg gtagaccaag atgagttagt aggatttatt ggtgcaatcc
    79741 ctcaatacgg tatcacaggt tgggaattgc atccattagt tgtagaaagc tcccgacgaa
    79801 agaaccaaat aggtactcga ttagtcaatt acttagaaaa agaagtagct tccagaggag
    79861 gaatcacgat ttatttaggt acggatgatt tagaccatgg aacaacgtta agtcaaacgg
    79921 acctgtatga gcatacattt gataaagtgg cttccatcca gaaccttcgt gaacatccgt
    79981 atgaattcta tgaaaaatta ggttataaaa tcgtaggtgt cttaccaaat gcaaatggct
    80041 gggacaaacc ggatatttgg atggcaaaaa cgattattcc tcgaccaaat tctcaataat
    80101 aaaaagaggt tgtgacagaa gtaatcagct tcaagaaata agaaggaatt tacgaaaatt
    80161 acttcttgaa tttttgtgaa attccaactt atttccgaag aagctacttt tggaacaccg
    80221 tgtac
//
LOCUS       DACECY010000005        77163 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.005, whole genome shotgun sequence.
ACCESSION   DACECY010000005 DACECY010000000
VERSION     DACECY010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 77163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.005"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            98..1162
                     /locus_tag="IWO38_000317"
     CDS             98..1162
                     /locus_tag="IWO38_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ0312435.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            1408..1833
                     /locus_tag="IWO38_000318"
     CDS             1408..1833
                     /locus_tag="IWO38_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0312436.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            1826..2275
                     /gene="nusB"
                     /locus_tag="IWO38_000319"
     CDS             1826..2275
                     /gene="nusB"
                     /locus_tag="IWO38_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ0312437.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            2372..3226
                     /locus_tag="IWO38_000320"
     CDS             2372..3226
                     /locus_tag="IWO38_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ0312438.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            3227..4564
                     /locus_tag="IWO38_000321"
     CDS             3227..4564
                     /locus_tag="IWO38_000321"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ0312439.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            4569..4793
                     /locus_tag="IWO38_000322"
     CDS             4569..4793
                     /locus_tag="IWO38_000322"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ0312440.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            4795..5685
                     /locus_tag="IWO38_000323"
     CDS             4795..5685
                     /locus_tag="IWO38_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ0312441.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            5694..6512
                     /locus_tag="IWO38_000324"
     CDS             5694..6512
                     /locus_tag="IWO38_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ0312442.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            6619..7068
                     /locus_tag="IWO38_000325"
     CDS             6619..7068
                     /locus_tag="IWO38_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ0312443.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            7086..8768
                     /gene="recN"
                     /locus_tag="IWO38_000326"
     CDS             7086..8768
                     /gene="recN"
                     /locus_tag="IWO38_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ0312444.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(8823..8939)
                     /locus_tag="IWO38_000327"
     CDS             complement(8823..8939)
                     /locus_tag="IWO38_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ0312445.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(9012..9968)
                     /locus_tag="IWO38_000328"
     CDS             complement(9012..9968)
                     /locus_tag="IWO38_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0312446.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            10139..10507
                     /locus_tag="IWO38_000329"
     CDS             10139..10507
                     /locus_tag="IWO38_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ0312447.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            10677..11108
                     /gene="mraZ"
                     /locus_tag="IWO38_000330"
     CDS             10677..11108
                     /gene="mraZ"
                     /locus_tag="IWO38_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ0312448.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            11129..12088
                     /gene="rsmH"
                     /locus_tag="IWO38_000331"
     CDS             11129..12088
                     /gene="rsmH"
                     /locus_tag="IWO38_000331"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ0312449.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            12093..12494
                     /gene="ftsL"
                     /locus_tag="IWO38_000332"
     CDS             12093..12494
                     /gene="ftsL"
                     /locus_tag="IWO38_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ0312450.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            12495..14687
                     /locus_tag="IWO38_000333"
     CDS             12495..14687
                     /locus_tag="IWO38_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ0312451.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGIAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            14713..15675
                     /locus_tag="IWO38_000334"
     CDS             14713..15675
                     /locus_tag="IWO38_000334"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ0312452.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            15689..17050
                     /locus_tag="IWO38_000335"
     CDS             15689..17050
                     /locus_tag="IWO38_000335"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ0312453.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            17076..18164
                     /gene="murG"
                     /locus_tag="IWO38_000336"
     CDS             17076..18164
                     /gene="murG"
                     /locus_tag="IWO38_000336"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ0312454.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            18179..19399
                     /locus_tag="IWO38_000337"
     CDS             18179..19399
                     /locus_tag="IWO38_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ0312455.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            19555..20880
                     /gene="ftsA"
                     /locus_tag="IWO38_000338"
     CDS             19555..20880
                     /gene="ftsA"
                     /locus_tag="IWO38_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ0312456.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            20903..22144
                     /gene="ftsZ"
                     /locus_tag="IWO38_000339"
     CDS             20903..22144
                     /gene="ftsZ"
                     /locus_tag="IWO38_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ0312457.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            22161..22838
                     /locus_tag="IWO38_000340"
     CDS             22161..22838
                     /locus_tag="IWO38_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ0312458.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            22852..23451
                     /locus_tag="IWO38_000341"
     CDS             22852..23451
                     /locus_tag="IWO38_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ0312459.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            23466..23753
                     /locus_tag="IWO38_000342"
     CDS             23466..23753
                     /locus_tag="IWO38_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ0312460.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            23802..24584
                     /locus_tag="IWO38_000343"
     CDS             23802..24584
                     /locus_tag="IWO38_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ0312461.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            24605..25312
                     /locus_tag="IWO38_000344"
     CDS             24605..25312
                     /locus_tag="IWO38_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ0312462.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            25574..28372
                     /gene="ileS"
                     /locus_tag="IWO38_000345"
     CDS             25574..28372
                     /gene="ileS"
                     /locus_tag="IWO38_000345"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ0312463.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(28421..29947)
                     /locus_tag="IWO38_000346"
     CDS             complement(28421..29947)
                     /locus_tag="IWO38_000346"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ0312464.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(29962..30609)
                     /locus_tag="IWO38_000347"
     CDS             complement(29962..30609)
                     /locus_tag="IWO38_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ0312465.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(30793..31122)
                     /locus_tag="IWO38_000348"
     CDS             complement(30793..31122)
                     /locus_tag="IWO38_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ0312466.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            31299..32027
                     /gene="sfsA"
                     /locus_tag="IWO38_000349"
     CDS             31299..32027
                     /gene="sfsA"
                     /locus_tag="IWO38_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ0312467.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            32043..33056
                     /locus_tag="IWO38_000350"
     CDS             32043..33056
                     /locus_tag="IWO38_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0312468.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            33056..34333
                     /locus_tag="IWO38_000351"
     CDS             33056..34333
                     /locus_tag="IWO38_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0312469.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            34396..37098
                     /locus_tag="IWO38_000352"
     CDS             34396..37098
                     /locus_tag="IWO38_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0312470.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            37250..37567
                     /locus_tag="IWO38_000353"
     CDS             37250..37567
                     /locus_tag="IWO38_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0312471.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            37597..37917
                     /locus_tag="IWO38_000354"
     CDS             37597..37917
                     /locus_tag="IWO38_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0312472.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            38025..39485
                     /locus_tag="IWO38_000355"
     CDS             38025..39485
                     /locus_tag="IWO38_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0312473.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            39553..39774
                     /locus_tag="IWO38_000356"
     CDS             39553..39774
                     /locus_tag="IWO38_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312474.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            39805..39987
                     /locus_tag="IWO38_000357"
     CDS             39805..39987
                     /locus_tag="IWO38_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ0312475.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            39987..40400
                     /locus_tag="IWO38_000358"
     CDS             39987..40400
                     /locus_tag="IWO38_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ0312476.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            40523..41704
                     /locus_tag="IWO38_000359"
     CDS             40523..41704
                     /locus_tag="IWO38_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0312477.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            42093..42166
                     /locus_tag="IWO38_000360"
     tRNA            42093..42166
                     /locus_tag="IWO38_000360"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42127..42129,aa:Arg,seq:cct)
     gene            complement(42235..43374)
                     /locus_tag="IWO38_000361"
     CDS             complement(42235..43374)
                     /locus_tag="IWO38_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0312478.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEVK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINAVFDSLYKNDN"
     gene            complement(43490..44125)
                     /locus_tag="IWO38_000362"
     CDS             complement(43490..44125)
                     /locus_tag="IWO38_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAQ0312479.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLILLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTAKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAQNNVLQAQTGYFGTETVSIALHN"
     gene            complement(44208..44630)
                     /locus_tag="IWO38_000363"
     CDS             complement(44208..44630)
                     /locus_tag="IWO38_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0312480.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFETEADRIAIMILLNIFIENELTDE
                     SQFKLENFMEFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            complement(44648..44968)
                     /locus_tag="IWO38_000364"
     CDS             complement(44648..44968)
                     /locus_tag="IWO38_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0312481.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            45706..45963
                     /locus_tag="IWO38_000365"
     CDS             45706..45963
                     /locus_tag="IWO38_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HAQ0312482.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(45934..46242)
                     /locus_tag="IWO38_000366"
     CDS             complement(45934..46242)
                     /locus_tag="IWO38_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312483.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            46320..47066
                     /locus_tag="IWO38_000367"
     CDS             46320..47066
                     /locus_tag="IWO38_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ0312484.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            47081..47284
                     /locus_tag="IWO38_000368"
     CDS             47081..47284
                     /locus_tag="IWO38_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0312485.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            47300..47638
                     /locus_tag="IWO38_000369"
     CDS             47300..47638
                     /locus_tag="IWO38_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAQ0312486.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(47893..48558)
                     /locus_tag="IWO38_000370"
     CDS             complement(47893..48558)
                     /locus_tag="IWO38_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312487.1"
                     /translation="MSLIDFIKQFDWNNIVKTIKDFILPITSLVFSTFAFYISYTDRK
                     NKKFNLKLDFFAECEEWLIDRESDSKPDVYHQNKFRIIDSVLLTNNSSLPVTIIEFSI
                     SGIPDPLNAFTMIGDNYSVTVKSMYDELPHGIRAYSGKSLKKGADLSKFPPLSLPITI
                     PPYESKITTLVFRYDESLVEKNITINVLTSRGTAKFNRFVSSSQISQLDTGYAPPQLD
                     EFD"
     gene            48613..49311
                     /locus_tag="IWO38_000371"
     CDS             48613..49311
                     /locus_tag="IWO38_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ0312488.1"
                     /translation="MQELVILKNKEAVTTSIQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFSEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNTKVMEVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDENE"
     gene            49489..49830
                     /locus_tag="IWO38_000372"
     CDS             49489..49830
                     /locus_tag="IWO38_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312489.1"
                     /translation="MEKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            49823..50494
                     /locus_tag="IWO38_000373"
     CDS             49823..50494
                     /locus_tag="IWO38_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ0312490.1"
                     /translation="MSENKGTTTFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            50500..51186
                     /locus_tag="IWO38_000374"
     CDS             50500..51186
                     /locus_tag="IWO38_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312491.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            51193..52044
                     /locus_tag="IWO38_000375"
     CDS             51193..52044
                     /locus_tag="IWO38_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ0312492.1"
                     /translation="MADNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDI
                     QSFIGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESS
                     GKTYKNYLATIRSWARKEKSESKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERAN
                     RQSKAPF"
     gene            52056..52325
                     /locus_tag="IWO38_000376"
     CDS             52056..52325
                     /locus_tag="IWO38_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312493.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            52487..52807
                     /locus_tag="IWO38_000377"
     CDS             52487..52807
                     /locus_tag="IWO38_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312494.1"
                     /translation="MDKKAAWRKLMLLIQDENWQEDEAVVAEVQRLEKIANGRIRKKP
                     DKRKLRKGKIIVVLYEGNILMQGTARELSAETEYTSGTIRTYAWRNHVDKKGHEYKYL
                     EGSK"
     gene            52804..53193
                     /locus_tag="IWO38_000378"
     CDS             52804..53193
                     /locus_tag="IWO38_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312495.1"
                     /translation="MIPKFRAWYTPFKGKTIGQEMKYGQAGRLITHAEMAPDKYVLMQ
                     STGLKDKNGVGIFEGDVVLVNVSNGFDHLVNEKTVVQESEFHSGLVCKSLISGMEYRV
                     FKHKKMDYEYEVIGNIYENSELLEEQR"
     gene            53190..53783
                     /locus_tag="IWO38_000379"
     CDS             53190..53783
                     /locus_tag="IWO38_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ0312496.1"
                     /translation="MNKQETIKKLESIKAIGNDAIAACYNESMNAGITLMKKIDEPQK
                     HVMPKFFDKWAKQVLEKRDKFYAISLIARAGWGYGVDFELSENGLSSENKELLYWLVD
                     KCSDNYPNKKKAIEALLYGYEVEKEPLYHVKLKIPGVTYYLIQTFLMPGDTHMCFSAA
                     TERYGAKWKNTFTESEIKAIDERYWPFAVPVEEEAEG"
     gene            53780..54157
                     /locus_tag="IWO38_000380"
     CDS             53780..54157
                     /locus_tag="IWO38_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312497.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQQIVFEWLKLTAPTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKEQFEVLAAFAQWGLEQEEAE"
     gene            54154..54357
                     /locus_tag="IWO38_000381"
     CDS             54154..54357
                     /locus_tag="IWO38_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAQ0312498.1"
                     /translation="MNNRHRRITKLRKQELNVLKAKFEKEYGVSAEEAYKVASQCVAD
                     ASNAIRKFGISILSDDRKWEDIE"
     gene            54354..54563
                     /locus_tag="IWO38_000382"
     CDS             54354..54563
                     /locus_tag="IWO38_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312499.1"
                     /translation="MKVYVVRFGNKFYRSDERSIGNNVLFLVDFLQNARWFDNLEEAN
                     QVSRRLGGLTQVYELVTIDHEGVER"
     gene            54560..54853
                     /locus_tag="IWO38_000383"
     CDS             54560..54853
                     /locus_tag="IWO38_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312500.1"
                     /translation="MKLKDGFYASSHGIGGLMLGMPTKNPKTRKKPKFKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKRLAKSKENLAVVRLVDMEVIKS"
     gene            54883..55323
                     /locus_tag="IWO38_000384"
     CDS             54883..55323
                     /locus_tag="IWO38_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0312501.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            55398..55865
                     /locus_tag="IWO38_000385"
     CDS             55398..55865
                     /locus_tag="IWO38_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAQ0312502.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGMSIYVTAQRNNTSEESVKAESNKIIIQFASSLELVAFK"
     gene            complement(56275..56628)
                     /locus_tag="IWO38_000386"
     CDS             complement(56275..56628)
                     /locus_tag="IWO38_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312503.1"
                     /translation="MAKIDDYRSDMIEYIELFAKNSNNNIIIQTGGATICGTPIDFDA
                     EVKVNPLIDAMMDSFAEFRSKKIDDIEKDDEQSLVVKSIFLKDVTIIGERTTNIPFLV
                     VFADQISAISLGNLE"
     gene            57559..57903
                     /locus_tag="IWO38_000387"
     CDS             57559..57903
                     /locus_tag="IWO38_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ0312504.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            57908..58189
                     /locus_tag="IWO38_000388"
     CDS             57908..58189
                     /locus_tag="IWO38_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312505.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNIFGYSIEEINYYIDFAEAFIPMVR"
     gene            58292..58606
                     /locus_tag="IWO38_000389"
     CDS             58292..58606
                     /locus_tag="IWO38_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAQ0312506.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            58584..60278
                     /locus_tag="IWO38_000390"
     CDS             58584..60278
                     /locus_tag="IWO38_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ0312507.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPGLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENAGTGFTPLVF"
     gene            60298..61476
                     /locus_tag="IWO38_000391"
     CDS             60298..61476
                     /locus_tag="IWO38_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0312508.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRAQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYKLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            61439..62125
                     /locus_tag="IWO38_000392"
     CDS             61439..62125
                     /locus_tag="IWO38_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ0312509.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKREEKIAAFTAAL
                     NKTFGQGDVK"
     gene            62125..63285
                     /locus_tag="IWO38_000393"
     CDS             62125..63285
                     /locus_tag="IWO38_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ0312510.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            63295..64170
                     /locus_tag="IWO38_000394"
     CDS             63295..64170
                     /locus_tag="IWO38_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312511.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            64167..64478
                     /locus_tag="IWO38_000395"
     CDS             64167..64478
                     /locus_tag="IWO38_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312512.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            64468..64821
                     /locus_tag="IWO38_000396"
     CDS             64468..64821
                     /locus_tag="IWO38_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ0312513.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            64811..65212
                     /locus_tag="IWO38_000397"
     CDS             64811..65212
                     /locus_tag="IWO38_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312514.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            65205..65609
                     /locus_tag="IWO38_000398"
     CDS             65205..65609
                     /locus_tag="IWO38_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312515.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            65621..66229
                     /locus_tag="IWO38_000399"
     CDS             65621..66229
                     /locus_tag="IWO38_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0312516.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            66249..66611
                     /locus_tag="IWO38_000400"
     CDS             66249..66611
                     /locus_tag="IWO38_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312517.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            66614..66796
                     /locus_tag="IWO38_000401"
     CDS             66614..66796
                     /locus_tag="IWO38_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312518.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            66813..70232
                     /locus_tag="IWO38_000402"
     CDS             66813..70232
                     /locus_tag="IWO38_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ0312519.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFIDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            70283..71020
                     /locus_tag="IWO38_000403"
     CDS             70283..71020
                     /locus_tag="IWO38_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0312520.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            71030..73321
                     /locus_tag="IWO38_000404"
     CDS             71030..73321
                     /locus_tag="IWO38_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312521.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            73345..75588
                     /locus_tag="IWO38_000405"
     CDS             73345..75588
                     /locus_tag="IWO38_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312522.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGNPNLLSKLSYDLIE
                     NQNTSAGTLSKGENSFKYNKISAETEGGVELYYKRRGIANWLPSNKTLVMTVKLRAGV
                     DYSPADGKKILIRYRFVDNGTGKIVLDLPINSNSITQEWKEFSITGTTPTFGPQAYHP
                     WIQFRAQDGILGEIEMSYDIKIEEGPTATPFQPNLLAEPYNMCREYPNENIVNPTVKF
                     PIKTSNNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQLQHQATLLP
                     VEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEKGNTRTPNIS
                     EYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQ
                     LTASINEVDTTAKDARTKANANATAIDELDNKIDERINDAATTAFYGAQGEVSNKTKV
                     AEYGVGTGYTTTAVGETHFERQSNGYVKCLKAGKYSISAQIRVQLGGKYASWLYTDLY
                     KDGTSVDNLVSYGVDALQNRFAASGNIVTDLEVNDVLNMRTEIGVADGILQFTSCRTL
                     VLTRLSD"
     gene            75666..75908
                     /locus_tag="IWO38_000406"
     CDS             75666..75908
                     /locus_tag="IWO38_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ0312523.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYELLREKAESAKDRADQVYSIA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            75924..76121
                     /locus_tag="IWO38_000407"
     CDS             75924..76121
                     /locus_tag="IWO38_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ0312524.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASALVAMLGKAYGWNGTDMIVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            76132..77151
                     /locus_tag="IWO38_000408"
     CDS             76132..77151
                     /locus_tag="IWO38_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0312525.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
BASE COUNT        26504 a        12531 c        16161 g        21967 t
ORIGIN      
        1 aaaaagaagt tttccggaaa tttttattta actgtcgtgt acgaagaaaa gtttgctata
       61 atggaaaaaa gcgtgcaaag aaagagggag tttcattatg atgctacgtg tagaaaaatt
      121 gcgcaaaaaa atgcaagaag aaaatttgga ttcatttttg gttaccagtc cgtacaatct
      181 tcgttactta acgaacttta caggtacgac aggattagct gttattactt tggaaaaagc
      241 attttttatt actgattttc gttataccga gcaggcggca gcgcaggctc aagggtttga
      301 aattattaaa aatgtcggac ctatttttga tgaggtagca aacttagtac gcaaagaaga
      361 actgcactca ttagcttttg aggaaacaac agtctcattt ttagaatatt cggttttaga
      421 agaaatcatc gatgcggaat tagtacctgt ttcacagatg atcgaagaac tcagagaagt
      481 gaaagatgaa gaagaaatcg ctattatcga aaaagcatgt catattgcgg atatggcata
      541 tgatcacatc ctgaaaatga tccaaccggg catgactgag attgaagtcg caaatcaact
      601 agacttttat atgcgttctc ttggcgcttc aggtgtttct tttgatacga tcgttgctag
      661 cggattgcgt tcggctatgc cgcatggtgt agcaagcaaa aaaatcatcg aacaaggtga
      721 cttgatcaca ttggactttg gttgctatta cgaagggtac gtttccgata tgacacgtac
      781 attcgctatt ggtgatcctg gggaaaaatt aaaagaaatt tatcaaatag tcttagatgc
      841 tcaattagca gttattgatg ctgcaaaacc aggaatgact ggtgtccaat tagatgcagt
      901 tgctagagat tatatttcaa aacatggtta cggggaagca tttggacatt ctacaggaca
      961 tgggatcggt ttagagatcc acgaaggacc aaacgtttct tctcgtgcag aaaagcaatt
     1021 tgtaccagga aatgtgatca ctgatgaacc agggatctac ttaccaggaa tcggtggagt
     1081 acgtattgag gatgacttgt tgatcacaga agatggaaat cgtgtgttga ctcgttcacc
     1141 aaaagaattg atcatcttat agtttcatca atagaagaag taagcgtaaa aatcagtgtt
     1201 cacagacttt gttatagaaa atcactcgat ttaatgatac actatagaag aacttttaag
     1261 ctgtaagaga taataaagtt gggtgttttc tccaattcat agcagtttgc agctgatcgg
     1321 cggcagtttt cttaccacgt gatcactatg ccgcatcata gagaattcgg atgtatctcg
     1381 tgcttaggca aagggaggaa taaataaatg gcagatgaaa aaaatttgat tttaaacgca
     1441 aaccaagatt taggagagat cgtgatcgct cctgaagtca tcgaagtaat tatcgggatt
     1501 gcagcttcta aagtagaagg cgtatacgga atgcgcggaa cttttgccag caatgtcact
     1561 gaattattag gacgtgcagc tcacggaaaa ggtgtttact tagcaaacga ggaagatggc
     1621 ttgaaagtag atctttactg ttatctagaa tatggtgttt cagttcctaa agtagcaatg
     1681 gaaatgcagg aacgggtcag ccagcaagtc cttttcatga cggatatcga tttatctgaa
     1741 gtcaatatcc atgtagtagc tgttgttcct gaaaaattag aacaaccaac aatcgaagaa
     1801 ctttttgaag acgaagagga agagaatgaa taaagaatta tcaagacacg agattcggga
     1861 aatggcattg caggctttat ttccattaga cttcaatgca gacctaacaa aagaagatgc
     1921 gattttcaac gcaatcgaat tagaccaccg cgacatgatc aacgaagatg aatcagagtt
     1981 tgttcctgtt tatcttgata cgctagttgg cggtgtctgt gcaaaaaaag atgaattgga
     2041 taaagtgatt gaaaaacatt taaaaaacaa ttggcacatc aatcggatct caaaaatgga
     2101 tttggtgatc ttgcgtatcg cgatttttga aatgacttat gtgacagatg taccagcggc
     2161 tgttgcttta aatgaagcaa tcgagttggc aaaaacattt agtgatgacc gttcacgtaa
     2221 attcgtcaac ggtgttttat ccaatgtatt gaaagaatta gaagctgggg cgtaagtcct
     2281 agcttttttc tatgaatcat cataaacctg ttataaatcg ggaaaattct atggtagaat
     2341 gaaaacacaa aaaatatctg ggagtggtta gatggcagaa ttgattaacg gaaaagaatt
     2401 agctgaaaaa atgcaggcag agattgctga aaaaatccaa aaattgaaag ataatggtat
     2461 ccatcccgga ttagtcgttt tattggtagg ggaaaatcca gccagccaaa tctatgtaag
     2521 aaataaagaa cgtgtagcaa aagaaatcgg aatccattca ttggtagagc gttatcctga
     2581 gacgatttct gaagacgaac tacttgcaga aattgaaaaa tacaatcaag atcctagttt
     2641 tcatggtata ttggtccaat taccattgcc aaagcatata gatgaagaaa agatcctgtt
     2701 agctatccgc cctgaaaaag acgtagacgg ttttcatccg atgaatttag gcagactcct
     2761 tgcgggtaaa ccggacaaga ttccttgtac tccatacggc atcatgaaaa tgtttgaagc
     2821 ttattctatc gatccggcgg gtaaacgagc agtagttatc ggacgaagca atatcgtcgg
     2881 taaaccgatg gctcagctac tcttgatggc agatgcaact gttactgttg ctcattctaa
     2941 aaccgaaaat ctggctgaac tggcacaaga agcagatatt ttagtcgtag caataggacg
     3001 tggacatttt gtcacaaaag aattcgtaaa accaggtgct gtagtgatag atgtagggat
     3061 gaatcgtgat gctaacggta agctgatcgg tgatgtgaaa tttgatgaag tggagcctat
     3121 tgcaggctgg atcactcctg tgccaaaggg tgtagggcca atgactatca cgatgctgat
     3181 gtatcaaacc gtaaaatctg ccgaagcttt tggagcaggt gaatagatgg cacaagaata
     3241 tttaactgtc actacattaa cgaagtattt gaaaagaaaa tttgatgccg atccttattt
     3301 agaacgcgta tatttaacag gcgaaatatc gaatttccgt ttacgtgcca atgcccatca
     3361 atatttcagc ttaaaagatg accatgcaaa aatatctgct attatgttca aaggagcatt
     3421 ccaaaaatta cgattccagc caaaagaagg aatgaaagtc cttgtcgttg ggcgtatttc
     3481 attatatgaa gcgggaggtt cttatcaaat ctatgtagag cacatggagc ctgatggaat
     3541 aggtgcttta tatcaagaat tggaagaacg aaaagaaaag ctgatgaagg aaggcctttt
     3601 tcaagggcct aaaaaaatgc tgccacgttt cccaaagcga atcgcggtcc ttaccagtcc
     3661 aagcggagcg gttatccgcg atatcatcac gacggtgaaa agaagatatc caattgtcca
     3721 attggttttg ttcccaacag ttgtacaagg aaaccaagca gcggacaatg ttgtcaggaa
     3781 tatccagaga gtagaagaga tgggcaattt cgatacaatg atcatcggtc gtgggggtgg
     3841 ttcgattgag gatttatggc cgtttaatga agagcgggtt gctcgggcga tttatcaagc
     3901 taatacgccg gtgatttcat ctgtaggaca tgaaacagat accacgatcg ctgatttagt
     3961 agcagatgtt cgggctgcca ctccaacagc agctgcagaa ttagctgttc ctgttttgtc
     4021 agaagagttg atgcgcattc aagaacggca agctagattg cagcaggcgt ttgtacgcca
     4081 gatccagcgc aaacaagaac ggttcgagcg tgctcgacgt tcctatgtat ttcggcagcc
     4141 tgaaagattg tatgaaggtc agaccatcaa attagaccag ttgaaacagc gattatatca
     4201 agctacacag caaatttatc atgcgaaaga aaaacaaacg atcgcattaa gccaccaatt
     4261 ggaacaagtg gctccgcttt atcgagttaa agcagctcgc caagaaactg cttatctaga
     4321 aaaacgctta gaagagaaaa tccaacaata tatgaaacaa cagcagcagc gttttgctca
     4381 agctgttcaa tctcttgatt tgttgagtcc attgaaaatc atgggaagag gctacagcta
     4441 tacgacgtta aatgatcaag tagtaaaaag tgtcaccgag attcatcaag gggatttgct
     4501 gacgatccat tatcaagatg gaatcgttaa aggacaagta caagagatag acaaaggaga
     4561 ataaaaatat ggaaaatcca acttttgaag agtcactgca agaattagaa aaaattgtca
     4621 tgcagttaga acaaggagac gttccattag aatcagctct agattcgttt aaacgaggaa
     4681 tggaattaag caaacattgc caagatacat tagctaaagc agaagagacc ttgacgaaaa
     4741 tgatgacaga atcaaatgaa gaagttgatt ttgacggaaa cgaggaaagc tgagatgaga
     4801 ttaactgact tttcagctat tcatctacct gctgtagaaa aagaaatctt gtctttttta
     4861 gatgagcata caacggatag aggactctat gatgcgatga gttattcagt gaaagcaggc
     4921 gggaaaagaa tacgtccctt attactttta acggccgttg cttcctttga tgagcctatt
     4981 gatgttcccg tctatcaagt agcagctgct ttagaaatgg tgcatacgta ttccttgatc
     5041 cacgatgatt tgcctgcgat ggacaatgat gatcttcgta gaggaaaacc taccaatcat
     5101 aaagtattcg gcgaagcatt agctatattg gccggagatg gtttgcttac aggagcattc
     5161 cagctaatca gtatggcaca cttaggtaat tctcctaagc ttctgctgct tcagcaatta
     5221 gctgtttgtg cgggatcaca aggtatggtt gctgggcaag ctgcagatat cgagggcgaa
     5281 tcgaaaaagc tttctttaga agaattagca tttattcatg aaagaaagac agggcatttg
     5341 attcgttatg ccttgctggc tggtggcata ttagcgaagc aacctgaaga aatcttattg
     5401 ctacttcaac gattagccga acatttaggt ttagcttttc agataagaga tgatctactc
     5461 gatgtgattg gtacaacgaa aacattaggg aaaacagctg gtaaagatga acgtatggaa
     5521 aaaaatactt acccgcgttt attaggccta gaaaagacac gtgaggcgtt ggagattgag
     5581 ctgttatccg ccaacaagat catagacaaa ttagaagagg acgttttttc ttttgatggc
     5641 gggctgatgc gccagatgat caagcaattt gatgtggaga aatgagggaa agaatggaaa
     5701 aagaacgcgt agatgtatta gcggtcaaac aaggattatt tgaaacaaga gaacaagcca
     5761 aacgttcagt gatggctggt ttgatttaca acgaaaaaaa cgagcggttt gataaaccgg
     5821 gagaaaaaat tccagtgagt agtgaattaa gagtcaaagg taaaaaactt ccttatgttt
     5881 ctcgaggggg attaaaatta gaaaaagcac tcaaagcatt tgatctatct gttgaaggaa
     5941 aaacattgtt agatattggt gcatccactg gcggatttac tgatgcagct ttgcaaaatg
     6001 gcgcaaaaat gagttatgct ttagacgttg gctacaatca gctagcttgg aaaataagac
     6061 aagatcctag agtcgtcgta atggaaagag tgaattttcg ttatgcaaaa cccgaagact
     6121 ttaccttagg aattccagaa gttgctgtta tcgatgtttc ttttatttca ctaaaactga
     6181 tgctgcctcc attacatgct attttaaaag aagagggaga agtgattgct ttaatcaaac
     6241 cccaattcga agcaggcaga gaagctgtag ggaaaaacgg aattgttcgt gaccctcaga
     6301 cccatcaaaa agttttagaa gacattttgt catttgcggc acaaggtgga tatgatgtgc
     6361 tggaattaag ttattctccg atcacggggg gagaaggaaa tatcgagttc ttggctcact
     6421 tgaggaaagt acccgaaagc ggaacaatca attcagctat taatatggca gaagtggttt
     6481 caaatgcaca cgaacagttt gaccataaat aaattgtaac tgtgaaaatc aagtgagaag
     6541 tatgcggatt cttctttatt ataccaagga aataagctat cattgattaa gaagaatatg
     6601 ctaaggagcg aagacgtaat gagaaaaaaa gatcggcatc gtttgattac tcgattgttg
     6661 agtgagcaag atatccgaaa gcaggaagaa tttgttgaaa tcctgaaaag caaaggaata
     6721 tctgttactc aagctacgat ttccagagat ataaaggaac tgaaactgat caaggtaccg
     6781 gcttcagatg gcggctaccg ttatagcata ccagctgaaa caaacgaaga tgtaaatact
     6841 aaattggaaa aactattaaa agacgctttc gtggcagttg accaaatgga aaaatttgtt
     6901 attttgaaaa cattgccggg aaatgcttcg gcagcagcaa atttgattga taaacgttac
     6961 aagaaagaat tattttcgat tatcaatgat gacgataatg ttttgatgat tacacgaact
     7021 gaagaccagg cagttttatt gaagaaagac ttcctgcatt acctttaagt tgaggagcga
     7081 atgtgatgct acaagaaatc agtattacta actttgcgat tattccggaa ttgcggcttt
     7141 cttttcacga aggaatgacc gcgctgaccg gagagactgg tgcgggtaaa tccattatta
     7201 tcgatgcttt aggtctgtta gcaggaggaa gaggttcaag cgattatata cgtcaaggag
     7261 cagaaaaatg tgtgcttgaa gggttgtttg aactaccaaa acaagaagga ttctctgaac
     7321 tgatggttga attagggatc gaaacagatg aggacaatct gattgttcga cgggatatgt
     7381 ccttaactgg aaaaaatgtt tgtcgagtga atggtcatat tatcacatta gctaatctga
     7441 gaagaatcgg cagttattta gtagatatcc aaggacaaaa cgaacatcag gaactgctcc
     7501 agcctgaatc acatcttgct ttattggatc gttttggaga tgtagctttc caacagaaga
     7561 aaaagagtta tcagcaagaa tatatctctt atcgtgagct ggaaaaaagg gtacgaaaag
     7621 tgcagcaaaa tgaaaaatcc tatgttcaga gaatagatat gctccatttc cagcaagaag
     7681 aaatagctgc tgctgatttg caagtcggag aagaagagaa attaaaagaa gaaagagaaa
     7741 aacttagcaa ttatcaaaaa attgctgatg gattagctgc aggttacgga gcactgacga
     7801 ataatgaaca aaacagtgta gatggtgttg gcttggccgt ttcagaactg caagggatcg
     7861 cccatctaga tgtggagtat gaagcaattt atgaaaatat ccaaagtgct tactatttgc
     7921 tgcaagatgc aatcggcgat atgagtcgtc agattgattt attggagtta gatgagagtc
     7981 gtttagaaga agttacgcaa cgtttagaat taatccgaca gttgaaacgt aaatacggcg
     8041 aatccattga gtcaatattg gcttactatg acgaaatcac cgaagagcta gcttcttctg
     8101 atttttcaga aggtcaattg gacaaaatga aagaggaact agagcaaaaa gagcttctac
     8161 ttcagcaaca agccgcagat cttcatgaag cgcgtaaaaa aatcgcaaaa gaattggaaa
     8221 aatccattct gcatgaattg aaaagtctgt atatggaaaa cactgagttt gaggtacgtt
     8281 tcttaaaaga agaaaataga caattgaatc atgatggatt tgaccaaatc gaattttaca
     8341 ttacgaccaa tccaggagag ccactgaagc ctttggtgaa agtcgcttct ggtggcgaac
     8401 tgtctcggat gttgcttgct ttgaaaacta ttttttcttc tgagcaaggt gtgacaagta
     8461 ttatttttga tgaagtggat acaggtgtga gcggaagagt tgcacaggcc atcgcagata
     8521 aaatttctca aatatcgaaa tactctcaag ttttgtgcat cactcatctg cctcaagttg
     8581 ctgcagtcgc cgactatcaa tattatattg tgaaagcagt gattgatgga cgtacgcaaa
     8641 cttctgtttc agagttgaaa acaaaagaaa gagaagaaga aattgcacgt atgctggctg
     8701 gaagtgagat tacaaaactc acactcgaac atgcaaaaga actattgaaa ctcgcaaaaa
     8761 ggacctgagc cacctcgcag tggttcaggt ccttttcagc agctgatagc tatattttat
     8821 ttttacatga cactcattac agcagtcaat acaacagatc caatcgttaa aatcagcatt
     8881 gtccaaatga caaattttgt tactttagca aaagtacttg tttgtttttt ttcacgcatt
     8941 gttttcaacc tcgtcattaa ttagttgatt caagtgtaca atcttttttc catttccaca
     9001 agagggcttt tttataaaaa cttccctttt tttaaataat aggtagttac gatacaaata
     9061 aataatgtta atagaactag ccatataaag gcatcatccc tttcagcaat aggtaactta
     9121 atgttcattc catagatact gccgacgatt gtcggtatag ttaaaataat cgttaaagaa
     9181 gttaaaattt tcataatatt attcagatta ttcgatacga ttgctgaaaa agtctgattc
     9241 atttgttcta ctagttttaa ttgaatgcga gaagtagtca ttgcctgctc tgtttccacc
     9301 aatacatcac gtaaatgatt tttcaaagca tgattttctt ggaaaagttt attttgcgct
     9361 agttctttca atgcatctaa attagcagaa gtcgcttctc tgaaataaac gaggcttttt
     9421 tggatatcca tgatttgata aagttgtttg ttttcagtgg atacttgaag tttggcttcc
     9481 aaagtatcgc attgagattg aagcgtagct aaataactgt tgtaagagag tgtgacctgc
     9541 caaatgaatt gaaagaagat attgagatta ggcgaaatat cactttttgg aaaggtctgg
     9601 ctaaagactt tctttaaaaa cagtggttca tgattcgtaa ttgtgataag cttctttttg
     9661 ggcgtcaaaa taatagagag cgggtatgta tccacttgaa aataaccgct aggacttgtt
     9721 ttagcaaaag gatattgtaa caataataac gctggctttg tcaactttga ttgattgaat
     9781 tcttctgtac gggaattttc tgcatcgtcc aatatggaag tgatgtaatc tgacggaatt
     9841 tcaaattgat gtgtcagctg atgaatctct tcttgattag gacgttctac acaataccag
     9901 atcgtttggt cagtttcctt tccggtatta cgcagtttct cattatctaa tgtaaaatat
     9961 ttgatcatga ttttcctcct gttgcctgtt tcttttatta taacggatag ggaattggaa
    10021 tacatggaat gtgtttgtaa gatatagaat ataactgcat aaatttaaat aaaatgaaac
    10081 agttgtgtaa agtatataca gtatacttga agcaacgata aaaaaggagt ttacgttcat
    10141 gggcacattc acaccactta tcttgttttg gtatatttac ccggtaatcg ttttgtttgg
    10201 ttgccaattt cttgtaaaat tattttcgtt aaatcgtcgt tttaaaataa aagcccctga
    10261 tttggcgatt ccttttttgt ggggcggtat gcatgcattg tctagaaata cagggacaat
    10321 ttctatctta ccgtttttct ttatttctgt tttgctaatg ggaatttgta ttgctgtttt
    10381 ccaagcatat tattatgaag aaattattta tccgcgttat ttcaagatga cttggcgttt
    10441 ggtttttctg ttgacaatcg tcttgtacgc tgtcttgatc attgtgaaca tactttctta
    10501 cctatgaaaa aaatagaaaa gctgtaaaaa gtcttttaaa aggctttttg cagcttgatt
    10561 tttaaaaaaa tgtttatgaa taaaaaaagt tgttcccgga tttcccagaa aaggtggtag
    10621 aaagtggggg attgtggtag actaatttta gttagtggag gaatgggggg ctttcaatgt
    10681 tgatgggtga attccaacat aatattgatg caaaaggacg tttaatcgtg ccctcaaaac
    10741 ttcgtgaaga acttggcgaa aagtttgttc tgacacgcgg attggatggc tgtcttttcg
    10801 gctatccgat gagcgaatgg gaaaatctgg aagctaaact gaacgaaatg ccgttggcta
    10861 aaaaagacgc acgtactttt gtgcgattct tctattcggc tgccactgaa tgcgaacttg
    10921 ataaacaagg acgaatcaat atacccagca cgttaagaaa ttatgcagct ttgacaaaag
    10981 gttgcgtagt catcggtgta tctaaccgaa ttgaaatttg ggatgagaca cgatggcaag
    11041 atttttctgc agcagcagaa gagaactttg acgaaattgc tgaaagtatg attgatttcg
    11101 gactataaga ataggaagag gctgactaat gactgaaaca tttcaacact atacagtcat
    11161 gttgaaagaa acggtagatg gattgaacat caaaccggat ggcgtttatg tagactgtac
    11221 actcggcggt gccggacata gtgaatattt attaagccaa ctaagttcta aagggcatct
    11281 gtatgcgttt gaccaagatc aaaaagcaat cgatcacgcc aagctgcgtt tagcttctta
    11341 tgtcgaacaa ggtatggtca catttatcaa agcgaacttc agagaattag aagaacgctt
    11401 gagtgagcat gtagataagg tagacggcat tttgtatgat ctaggggttt cctctcccca
    11461 gctagatgaa gcagaacgtg gatttagtta tcatcaagat gcaccgttgg atatgcgcat
    11521 ggatcagtca gctcctttat ccgcttatca cgtaattaat gaatattcct accatgaatt
    11581 agtaaagatt ttcttccgct atggcgagga gaaattttcc aaacaaattg ctcgtgaaat
    11641 cgaacgagta cgaaaaacaa aaccaatcca aacaactggt gagcttgtcg aaatcattaa
    11701 acaagtcatc ccagctcccg caagaagaaa aggcgggcat cctgcaaaaa gaatttttca
    11761 agctgtgcgg attgcagtaa atgacgaact cggtgccgaa gaggcctctt tagaacaggc
    11821 aatccggtta ttgaaagtca atggaagaat cagcgtaatc acattccatt cattggaaga
    11881 cagaattgtc aaaagtatgt ttaaagaata tagtacggta caagatctac caccgggact
    11941 tcctgtcata cctgacgaat tccaaccaga actgaaagtc attactcgta aaccaatcct
    12001 tccttcagag gaagagctaa cagaaaacaa tcgatcacgc agtgcaaagt tacgtattgc
    12061 agaaaaaata agaatgaaag aggagtagaa tgatggcaga attaaagaaa atgcaagatt
    12121 acgaatatga tttacctgaa ttggtggatc agccggaaca acaaatgcct gattcttctt
    12181 cgaatcaaga aatcattctt ccacaatcac cggcaaaacg attaaagcag atttctgctt
    12241 tggaaaaaat tgctgtggca agcgtggcga ttgctattgt tgctttatgt gttttaacag
    12301 ttatgctgcg cacaaatatc agcggagctg aaaaagaaat cacgtcaata caaacagaaa
    12361 tcaatgataa aaagcaagaa aaaacgagtt tagaacaaga aaaaaacgaa ctttctcgta
    12421 ctgaacggat taaacaaatc gcagaagaaa aaggattgtc tattaatgac gacaatttaa
    12481 ggaaagtgaa gtaaatgagt cttaaaaata aaattcggcg gtttatggaa aagaaaaatc
    12541 taaatccgat gaacaatcgc aaaaaagtag gtatcatttt atttgctacg agtattggat
    12601 tgttcttttt atttgccgtc cgtttctcgt atatcgttat tggaggtcat gttgctggta
    12661 cctcattagc tgaaaaaaca aaacagttgt accaaggaag tgaagtagta aaagcaaaaa
    12721 gaggaacgat ttatgatcgt aacggtgtgg cgctcgcaga agatgcctcc tcttattcaa
    12781 ttaaagcgat actctcaaaa acatatacct ctggggataa aaaactgtat gttgaagaaa
    12841 agaattttga taaaatagca gaaatcctgc ataagaatct ttctatagac aaaaaagatg
    12901 cactgaatat tttagaggac ggcgcaaaaa aagagctgta tcaagttgaa tttggtagct
    12961 atgggaaaaa tatctcccaa gaaaccaagc aaaacatcga agcggacatg aaaaaagaag
    13021 gtgtcgctgg tctttatttt gtcgaccatc aggctcggat gtatccaaat ggcgtctttt
    13081 cttctcattt tattggatac gcagttccag acaaagatga gaatggctta gttggtaaac
    13141 taggtttgga gtctgcttac aatgatatct tgagcggtaa agatggtaag attatttatc
    13201 aaaaagataa ttttcaaaat cctctgccag gtacagtggc agaagaagaa aaagctgtcg
    13261 acggacaaga tatctacacg actttagaca gcagactaca aagttattta gaaacactga
    13321 tggatcaagt aaatgaagaa tatcagccag aagaattgac agcagtcctg atgaaagcaa
    13381 aaaccggaga aatcttggca atgggacaac gacctacttt caatcctgaa acaatggaag
    13441 gattgactgg aaaagatgct atctggcgta atttcttggt acaagatagt tatgaaccag
    13501 gatcaaccat gaaagttttc actactgcag ctgctatcga agaaggagaa ttcaacgaga
    13561 atgaaacctt ccaatctgga aaaatccaag tagaagatgc tacgatcaat gaccatgact
    13621 ttggtgaaaa aggtgtgctg accatgcgtc aagctctttc atggtcaagt aacgtcggga
    13681 tggtcatact ggaacagcga ttaggcggac gatggtataa ctatttacaa aaactgggat
    13741 ttggacaaag tactcattct ggattagatg atgaagtaaa cggtgcttta cctacattaa
    13801 atattgtcga ccgagcaatg agtgcttatg gacaagcggt aggtgtgacg aatttccaaa
    13861 tgatgaaagc tttcacttct attgcgaata acggtacaat gattcaacct cgatatatca
    13921 gcaaagtggt ggatccccaa actggagaag agcgaacaac tcaaacagaa gttctagggc
    13981 aacctttttc aaaagaaaca acagaaaaag tccgtgaata tatgagagat gttgtcgaaa
    14041 gcgaaaacta cggtattgct tatggtgtat atagtgtacc tggatacaat gtatcggcaa
    14101 aaaccggaac agcccaaatc gcttctgata cagggggata tcaaacagga gatacagcct
    14161 acctgtattc gattgtggag atggttcctt ctgaagatcc agactatgtc ttgtatctaa
    14221 caatgaaaca ccctaagaca tatgatcgaa tggcattggc taaaatcgca aatccattga
    14281 tgaaacgggc aatggatttt aaagaaactg aagaagattc agatacagaa acaaaaacag
    14341 aaaaagtatc cgtagccgat tatcgtaatc ttgaagcgga cgttgcagca gctgatgctc
    14401 aaaaaagtgg actgcaacct gtagtgatcg gtaatggtaa aaaagtacaa aaacaatcta
    14461 ctgccaacgg tgatcagttg atctctggag aaaaactgat cctatatact ggtggtgata
    14521 agctgatgcc tgacgtaaca ggatggtcaa aagcagatat catgaagctg gggaaaatcc
    14581 tcggtattga agttagtttt gatggagatg gttattgtgt caaacaagaa cttgctccat
    14641 atgaaaaaat cactgaagac aaacttagtt ttactttaga agaataagaa gagaaatagg
    14701 agagaaaaga gcatggaatg gacacaagca ttgattccca ttgtcagcag ttgtgcaatg
    14761 acaattgccg caatgccgct atttattggt tattttcaaa tgaaaaagca aggtcaggcg
    14821 attcgtgagg agggaccaaa gtggcacaat agcaaagccg gaactcctac aatgggcggc
    14881 ttagtgtttc tgatcggctc tattttgaca ggaatttggg ttggagcttg gcaaaaacag
    14941 ctaactccta ctcttttcat cttgcttttt gtattagcgt tatacggagt aatcggcttt
    15001 cttgatgatt ttataaaaat attcaagaaa agaaacatgg gactgaactc gaagcagaaa
    15061 ttgattggac agattctagg gggaatcatc ttttatcttg tttaccgttc tgaaggttat
    15121 ccaggtgtat tgaatttctt cggactagat ctacctttag gaatcattta tggtattttc
    15181 gctctcttct ggcttgttgg attttcaaat gcagtaaatc tgacagatgg catcgatgga
    15241 ttagttgctg gcttaggaag tatctctttt gcagcttacg gtctaattgc ttggcatcag
    15301 catcagtatg atgtattaat catttgctta agtgttttag gaggtttact gggtttcttc
    15361 gtctacaaca gaaaacctgc caagattttt atgggagatg tcggttcatt agcgttgggc
    15421 ggacttttag cggcaatttc tataatgcta aaccaagaat ggacattgct tttgattggt
    15481 ttgatctatg ttatggaaac agcaagtgtg atgcttcaag ttacttcctt taagcttaca
    15541 ggaaaacgaa tttttaaaat gtctccaatt catcatcact ttgaaatgtg cggatggtcg
    15601 gagtggaaga ttgatacagt tttttggttg atcagcattg tgacttcgtt gatcacatta
    15661 tggatcgttt ggtaggagat gacgggaaat gaaaaaaata tcaacatatg aaaataagaa
    15721 agtgcttgtc ttaggactcg caaaaagcgg agtaagtgcg gccaaattat tacatgaatt
    15781 gggcgcactt gtcaccgtta atgatggaaa accttttgac gaaaatccgg aagcacaaga
    15841 gctgctttct ttagggataa aagtaataac aggtagtcat ccaatcgaat tgttggatga
    15901 agaattttca ttgatggtca aaaatccagg gattccctac agtcatcctt tagtagcaaa
    15961 agcccaagaa atgggtattc cggtaattac ggaagttgag ctagcttacg aagtagcgga
    16021 atgcccaatt attgggatta caggaacgaa cggaaaaaca acgacaacaa cgatgacagg
    16081 actactactg aatgcaggag cagatcaagg aattgcacgt ttagcgggta atattgggta
    16141 tccagcaagt ggtgtagcgc aagaagcaaa atcagaagat aaaattgtga tggaattgtc
    16201 tagcttccaa ctcatgggaa tcacagattt ccgccctcat atagcggtga ttaccaatat
    16261 ctatgaagca catattgatt atcatggtac aagaaaagaa tatgtcaaag ctaaatggaa
    16321 ccttcaaaaa aacatgacag aaaaagatta tctgatcctt aactggaatc agtcagaact
    16381 acaagaatta gcgcaaagaa caaaagcacg cgtactgcct ttctcgacaa aagaagtgtt
    16441 ggaagacggt gtgtatgctg acgattactc aatatactat aaaaaagaaa aaataatgga
    16501 aatatctgaa cttggcgtac caggtaagca taatgtagaa aatgcactag ctgctatttc
    16561 tgtagcaaaa ttgtatggta tatcaaatga agctatacga gaaacgttgc attttttcca
    16621 tggcgtacca catcgtacgc aatatgtagg agaaatccaa ggcagaaaat tttataatga
    16681 ttcaaaagca accaatattt tagcgactaa aatggctcta agtggatttg aaacgtctaa
    16741 ggtcgtgtta ttggctggcg gattagacag aggaaataca tttgatgaat taattccttc
    16801 attaaaagga atcaaagcaa tggtcgtatt tggccaaaca aaagaaaagt tgatggatgc
    16861 tggaaaaaaa gcaggcatcg agacaatagt aacagccgat tctgtagaac aagcagttcc
    16921 tttagcatta gaaaacagta cagatggtga tgtagtttta ttatcaccag cgaatgccag
    16981 ctgggatcaa tacccaaatt ttgaaactag aggaaacaga ttcatggaag ctgtgaaccg
    17041 attaaaatag aaaaagtaga agcgagcgat aaaaaatgaa aattttggtt acaggcggag
    17101 gaacaggggg acatatttat ccagctcttg cctttgtcaa ttatgtaaag actcaagaac
    17161 cgaatgctga attcatgtat gtgggagcaa aacgaggtct agaaaacaaa atcgtccctg
    17221 atacaggaat gcctttccat accttggaga ttcaaggatt taaacgaaaa atttccatgc
    17281 ataacatcaa gacggtccga ttgtttctta agagtatccg tcaagcaaaa aaaatcctca
    17341 gtgattttca accagatatc gttatcggta ctggaggata tgtttccgga gcggtagtgt
    17401 acgctgcagc caaaatgggt attccaacag ttatccatga acaaaacagt gtaccaggtg
    17461 ttaccaataa atttttaagc cgatatgtgg atcggatcgc tctttcattt aaagatgcgg
    17521 cttccttttt tcctgaaaac aaagcagttc ttgtaggtaa tcctcgagca caagaagtag
    17581 cagatactaa aaaatcagat attttgaaaa catttgattt ggatccagaa aagaaaacag
    17641 tcttgatttt tggcggtagt caaggagcac ttaaaatcaa tcaagcagta tcagtttttt
    17701 taagtgaata tattccttct tcttaccaag ttttgtatgc ttcaggagat agatattatg
    17761 aagagatcct aaataaaata aatcctgaaa tacaaaatat tagtgtccag ccttatatca
    17821 aaaatatggc agaggtcatg gcttccagtg atctgctggt ggggcgtgct ggtgcaactt
    17881 cgatcgcaga attcacagct ctggggttac cggcagtgtt ggttcccagc ccatatgtta
    17941 cgaatgatca tcagactaaa aatgcaatga gtcttgttca cgcaggagca gcaaaaatga
    18001 ttgcagataa tgaactaaca ggtgaatcac taagtcagac agtcaacgaa atcatgggtg
    18061 atgaagaatt gcaaaagcaa atgtgccgag cttctaaaga acaaggaatc cctgatgctt
    18121 caaaacgtct ttatgactta gtaaaacaaa taatcaaaaa ataaaggcgg tggtcttagt
    18181 gagtaagaaa aaagaaccaa aaaaattgtc tcatccgtct tctgatgaaa acaatttgac
    18241 tccttggcaa aaggctaacc gaaaatatct ggcagaacaa gagatgaaaa accaagagcc
    18301 aaaaaaagaa gacataacat tagatacacc cctttcagat tctgaagaag aaaaaacggt
    18361 tcaagaggaa cagcaagaag aaagccagtc cgagaaaaaa atcgaatttt ttgaagagat
    18421 caagcaaaag aaaaacggtg gaccatataa cggttctttt ttgaaccggc tgcccaattt
    18481 aaaaagtcaa cggcacaaag tactatttcg tcggttagct ttgatcatca ctgtattggg
    18541 tataccgctg atttttttga tctattatgt ttctccttat agcaaattac aagcagttgc
    18601 agtatcaggc aataagacag tcaacagcca agaaatcatc tctgatacaa aattgtcatt
    18661 aggagaaaat gtttggggac agtattttca tcgatctact tatatcgatc gccttaaaaa
    18721 agcacagcca agaatcgaaa cagcaaatat acatttcaaa gggatgaatg aattcgattt
    18781 ggatgtaaca gaatataaag agattgcttt gatcgcaaaa aacaatcaat actacccagt
    18841 gattgaaaat ggaacagtat tagatgaaaa agtagcaaat cctacaaaaa atctccctat
    18901 cttggaagag ttcaaagact ctgcaaaaat caaagaatta acaaagcaat acaatcagtt
    18961 atcatcagaa ttacaaaaag caatttctga aatcaagtac acgccaagag caagcaataa
    19021 aaatctgatc caattgaata tgaatgacgg caatcaagtt attgtaaata tcaataatct
    19081 tgcgaaccaa atgaaatatt attcgcaagt agccaaagat atggatgaaa aaggtgtgat
    19141 cgacatggaa gttggtatct tctcgcatcc ttacggtagc actcaagaaa cggaagaatc
    19201 gggccaatcg gaagaaacaa ctgaaagtga agaaaatgcg gatcaaacaa acgaatcaag
    19261 tgaagaggca gaaacgcaaa aagcacaaac acctggatcg tctagcacaa acggatcatc
    19321 tgattcaaat gattcaagtg aaacaagtca aatagaagat aatccaagta caactccgtc
    19381 aagtttaaat gaaaattgat aaaacaactt taaaaagtaa gaatgtactt tctaaaaacc
    19441 agtaatttat ggtagaattg gaagcagtga atatatctat taataagtat attttaaaac
    19501 ggacgctagt tgttcgcttg tatatggaat agtaagtagg agggaatccc attcatggca
    19561 aaaacaggaa tgtatgtagg ccttgatatt ggaacgacat ctgttaaagt tgtcgtggct
    19621 gaatatatcg acagccaaat gaatattatt ggagtaggaa acgcaaaatc agaagggatt
    19681 aaccgaggca ttatcgttga tatcgataaa acggtccaag cgatacaacg agcagtacga
    19741 caagcagaag aaaaagcagg tatccagatc aaaggcgtaa gtgtcggttt gccagcaaac
    19801 atgcttgaag tagaaaattg ccagggaatg attgcagtta acggagattc aaaggaaatc
    19861 accgatgaag atgtcagaaa tgtggcttca gctgcccttg ttcgttcaat acctcctgaa
    19921 cgtcaaatcg tgtcaatatt gccacaagat tttactgtcg atgggttcga gggaatcaaa
    19981 gatcctcgtg gaatgatcgg cgtaagatta gaaatgtacg gtctgttgtt tacaggacct
    20041 aaaacgatca tccataacat acgtaaatgc gtggaaaatg ccggattaat cgtcaacgaa
    20101 atggtgatta ctcctttagc gctgactgaa tcaattcttt ctgacggaga aaaagatttt
    20161 ggtacgatcg tcattgatat gggtggcggc caaacaacaa cggctgttat gcatgataag
    20221 caattgaagt ttaccaatct tgaccaagaa ggtggcgaat ttgtcactaa agatatctct
    20281 attgtattaa atacgtcatt taacaatgca gaagcgttga aaataaacta cggggatgca
    20341 tacccagaac ggacatcacc agatgaagaa tttccagtag atgtgattgg tcagtcagaa
    20401 ccagtgaaag tcgatgaacg ctacttgtca gaaattattt cagctcgaat ggaacagatt
    20461 ttcaacaaag caaaagatgc attggaccaa atcgaagcat tagagttgcc aggtggcgtc
    20521 gtattgacag gcggagctgc tagtcttcct ggtgttgtgg acttggcaca agaaatcttc
    20581 ggcgtaaatg taaaattgta tgttccaaat cacatggggc tgagaaaccc ggtatttaca
    20641 aatgtaataa gtatcgtgga ttattcagca aacttgagtg aagtgtatca attggcaaaa
    20701 attgctgtta cgggtgaaac ctcagcagct cgtcaaatgg tagttgaaca agaaacaaca
    20761 aacacatatg agagctatga agcacctgaa gaaacaatct acgatgagcc ggaaccaaaa
    20821 gagtccggag aaaatgtaaa aaataaaatc aagggtttct ttacaaatat ttttgattaa
    20881 ttcatcctgg gaggaataag atatggaatt ctcaattgat aataatatca atgacggcgc
    20941 agtaatcaaa gtcatcggag tcggtggtgg cggtggaaat gctgtaaacc gaatgattga
    21001 agaaaacgtt aaaggtgttg aattcattac agccaacaca gacgtgcaag cattaaaaaa
    21061 ttcaaaagct gaaacagtta tccaattagg ccccaaatat actcgtggtt taggtgctgg
    21121 ttctcagcca gaagttggac aaaaagcggc agaagaaagt gaacaatcct tacgcgaagc
    21181 tttagacggt gcagatatga ttttcatcac tgcaggaatg ggtggaggaa ccggtacagg
    21241 tgctgcacca atcgttgcag gtatcgcgaa agaattgggc gcattgactg ttggtgtcgt
    21301 gacacgtcca ttcacttttg aaggaccgaa acgtggccgt tttgctgcag aaggtatcgc
    21361 tcgtttgaaa gaaaatgtgg atacattatt aattatctca aataaccgtt tattagaagt
    21421 tgtagataaa aagacaccga tgcttgaagc tttccgtgaa gcagataatg ttttacgaca
    21481 aggtgtacaa ggtatctctg atttgatcac agcgccaggt tacgttaacc ttgactttgc
    21541 tgacgtgaaa acagtgatgg aaaaccaagg tacagcattg atggggatcg gtgtggctag
    21601 tggagaagat cgcgtagtag aagcaacgaa aaaagcaatc tcctctccat tgcttgaaac
    21661 atctattgat ggagctgaac aagtacttct taacatcaca ggcggtttgg atatgacatt
    21721 gttcgaagca caagatgctt cagatatcgt agcaaatgca gcaactggcg atgtaaatat
    21781 cattctaggt acatctatca atgaagaaat gggcgatgaa atccgtgtga cggtcatcgc
    21841 aacaggtatc gatgaatcaa aaaaggaacg caagtctagc cgaccagcta gacaagcaca
    21901 aatgcaatca cctgctcaaa agactgtatt agatatggat caagctaaac caacttcttc
    21961 agaagaagaa aacagttttg gcgattggga tatcagacgt gaacaaaatg ttcgtcctag
    22021 agttgatgat tccaattttg acaatatcga aaaaaaagaa tttgatacat tcaaccgcga
    22081 agaaacaaaa tctcatggcg atgatgaatt aaatacgcca ccgtttttcc gtcgtaaaaa
    22141 ataaaggaga ggaagagcac atgattgctg acaacttgca gaaaatcaag caagaaattc
    22201 aggattcgtg tgctcttgtt catcgtgatc cgaaagaagt tacattagta gctgtaacaa
    22261 aatctgttga atccgctcaa gctgaagaat tggttagagc tggcgttatg aattgtgcag
    22321 aaaaccgagt agataaatta ttagaaaaga aacaagattt atttgatttt cctgaaataa
    22381 agtggcattt aattggtaat ttacagcgca gaaaggtaaa attgattgta aatgaaattg
    22441 attactttca tgcgttggac agtttaagat tagcagaaga aatccagaag cggttagaga
    22501 aagaattatc ctgttttatt gaagtaaatg tttctggtga ggaatcaaaa catggaattt
    22561 caccagatga gcttttaccg tttgtggaat ctttagctgc ctgcacaaaa atcaaggttg
    22621 ttgggttgat gactatggcg cctaaagaag cttctgatga gcaaatccat aaagtgtttg
    22681 gtacgctgaa agaattgcgt gatcttgttc aagcaaaagg ttatgcccat gcaccatgta
    22741 gtgaattaag tatggggatg agtcaagact ttcaaattgc gattgaagaa ggtgcaacat
    22801 ttatccgtgt aggaacagcg ctattcaaag acgaataaag gaggaacaaa aatgccgctt
    22861 ttaaacagag gagcaattgc taattttttc ggattagctg atgaggaaga gtacgaatac
    22921 caagattatc ctgaaacaac aaaaaaggct gtacagccaa ctaaaaaaaa tacaacacag
    22981 aatcaatcaa ctcattctgt atctgctgcg aatactcatg aaaaacaaaa aacggcgact
    23041 tcacgtcctg taacgcatga aagtattcag ccggctgaaa agaaaaaagt aattgaacta
    23101 catcaatcac ctacaaaaac tgtagcgaaa gaaagaacag cttcaagaac ctctacacaa
    23161 cctaaagcaa atcttgctgg aaaaatcact gtaatggaac caagagctta ttctgaagct
    23221 atggcgattg ctaaacgaat cattgctgga gaagcaacct tagtgaattt tcatttagta
    23281 gaagaaaaac aagcgagaag aatcgttgat tttctaacag gaacagttta tgcactagat
    23341 ggagatatca aacgtgttgg agatgagatt tttctttgta caccgactgg gatggagatt
    23401 gacagttcaa ccgctcaatc ttttgcagat gcgaatttgt ttgatctgta agaggaggaa
    23461 ctacgattta tactattgca ctattacttc atttacttca acgggcagcc tatttctata
    23521 gcattatctt gatggtatat gcattgctct cttggtttcc aggcgggtat caatcagggc
    23581 taggacgatt attagctaag atctgcgaac cgtatatcag tttgttcgat cgtctgcctt
    23641 tgaggattgg acccttggat ttcacaattt taggggcaat cctgatcttg aacttagccg
    23701 tacaaggttt agcacagatc gttatatgga taactgtcca tttagtttat tgattgaagc
    23761 aacaaaatct ttattctcag aatagatagg aggggaaaat tttggatgca aatgtatacc
    23821 agcactttcg ggcagatgaa cgtacattta tcgattcggt aggcgattgg attgaacagg
    23881 ttcaaggaca atatgcaccc tatctgaccg aatttcttga cccaagacaa tcctatattt
    23941 tagaaacact gattcgacaa agcagtgatt tgcgtttcat gttttatggc ggatatgaac
    24001 aggcagaaag aaaaagatgt ttgattttcc ccgattatta cgaacctgta gaggaagatt
    24061 ttgaaacagc attgtatgaa atccattatc ctagcaaatt tgccagccta agtcatggga
    24121 aaatccttgg aactttagtg ggaacgggga tcaaacgctc ttactttggc gatatcatct
    24181 cggacggtga tcgctggcaa gtatttattg caaatgaggt ggatcatttt gttgctgcac
    24241 aagtaacaaa gattggaaag gttgctgtac gcttggaacc aatcaaatac acagaaatga
    24301 ttatgccgaa agatagctgg acacaagaaa gaggaacagt tagttccatg cgactagaca
    24361 gtgtgatctc tgagatttat aatatttcca gacagcggtc caagcagctg atcgaagctg
    24421 gtaaagtaaa agtgaactgg acagaaaata caagacctga ttttgtactt gaactattag
    24481 atattatttc agtcagaggt tatggcagag tccagatcca agaacttgag gggaagacga
    24541 aaaaagaaaa gtacagggta ctgttcggtg tattacgtaa ataaaataaa aaggtggttt
    24601 actcatggct ttaactccat tagatatcca aaataagaca ttcccgacaa aaatgcgggg
    24661 atataatcaa gacgaagtag atgatttctt agatttagtt gttcgtgact atgaggaatt
    24721 gactcaaaga aatcgagaat tagaaaaagc agtcaaacat tctgaagaaa aacttgagta
    24781 cttcaatgaa ttaaaagatg ctttgaacca atcaatcatc gtagctcaag atacggctga
    24841 taaagtaaaa acaagtgcaa gcaaggaatc cgaagtgatc gttacttctg cacaaaacaa
    24901 agcagatgag ttagttgcga acgctgaaaa acgtgcacat caattaacaa ctgatgcaga
    24961 agaaaaagca agaaaaatct tgacagatgc aacagaaaaa gcaagacagc ttgcgacaga
    25021 aactgaagat cttaagaaaa aaacacgagt attccatcaa cgtatcagct tgatgcttga
    25081 gtctcaatta gaacaagtga aaagtccgga atgggatgaa attttacaac cattctccag
    25141 ctacgtgact gacagccatg aagtgatcaa agaagtttta gcaaaacagc ttgacaatga
    25201 aaatgatact gatgtaaact cagacacagg agtcatagaa gcacctatga cggcatttga
    25261 tacacaggct attgatatct cgatcgtgcc tggtgaaaat gaaaacgagt agaacagaaa
    25321 agcactttgt tattcttaaa gcgagccgat gagagtggaa gttcggtaag acactgcatg
    25381 gtgaaagatc ctctacttga gacctgcttt tccgaaatta gagtaagaag cagcggctga
    25441 cctcgttatt ggtcacagag agtttgcact agatagatgc aactaaattt gggtggtacc
    25501 acggcatgtt cgtcccttgc ggctgaacat gcttttttgt gtccggaaaa caaataaagg
    25561 agtgtatggt tgaatgaaaa tgaaagaaac cttgcaatta ggaaaaacaa aattccctat
    25621 gcgtggtaat ttgccaaacc gagaaggcga gtggcaaaaa gaatgggaag aaaatcgaat
    25681 ctaccaaaaa agacaagagt tgaacgaagg aaaacctagc tttgttcttc atgatggacc
    25741 tccatatgcc aatggtaata tccatttagg acattcattg aataaaatca gtaaagacat
    25801 tatcattcgt tcaaaatcaa tgtctggcta tcgtgcacca tatgttccag gatgggatac
    25861 acatggattg ccgattgaac aagtattggc taataaaggc gtgaaacgta aagaaatgtc
    25921 catggcagaa tacttggaaa aatgtcgtga atatgcactt tcccaagtag ataagcaacg
    25981 agcagatttc aaacgcctag gagttgcagg agattgggaa catccttatg ttacattaga
    26041 tccttcttat gaagctgcac aagtacgcgt atttggtaaa atggctgaaa aaagctatat
    26101 ttacaaagga ttgaaaccaa tttactggtc tccgtcaagt gagtcttctt tagcggaagc
    26161 tgaaattgaa tataaagatg taaaatctcc atctattttt gtagctttca atgtcaaaga
    26221 cggcaaagat attcttgatg aagatacagc ttttgtcatt tggacgacaa caccatggac
    26281 attgccagca aaccaaggta ttgctgtaaa tccaaaattt acgtatgtat tggtggaagc
    26341 cgatggccgt aagtttgtcg tagcgaaaga tttgctagaa acagtccaat cagaaatcgg
    26401 ttgggaaaat gtaacagttc taaaagaatt tgctggacaa gaaatggaat atatgactgc
    26461 aagtcatcca ttctatgaac gtgaatcact tgtgatttta ggcgatcatg ttactttaga
    26521 cgctggtact ggattagtcc atactgctcc aggacatggg gaagatgact atatcgctgg
    26581 tagcaaatat aaactatctg tagtgtcacc tgtagactca aaaggattgt ttacagaaga
    26641 agcacctgga tttgaaggta tcttttatga caaagcaaat cctatgatca ctgatctatt
    26701 aaaagaaaaa ggagctttgc tgaagctaga tttctttact catagttatc ctcacgattg
    26761 gcggacgaaa aagcctgtga tctatcgggc aactccacaa tggtttgcat ctatcgataa
    26821 attccgtcag aatattttgg atgaagtaga aaaagtagag tgggttatcc catggggtaa
    26881 aacacgtttg tataatatga tccgtgaccg cggcgattgg gtaatttccc gtcagcgtgc
    26941 atggggagta ccacttccga ttttctatgc tgaaaatgga gaagcaatca tcacaccaga
    27001 aacaatcgaa catgtggcaa aattatttgc tgaacatgga tcaattatct ggtttgaaaa
    27061 agaagcgaaa gaacttcttc ctgaaggatt tacccaccca ggttctccta atggcgaatt
    27121 tacaaaagaa aaagatatca tggatgtctg gtttgattct ggttcttctc atgaagctgt
    27181 tttacgtcag cgaccagaat tatcattccc agcagatatg tatttagaag gatcagacca
    27241 atatcgtggc tggttcaact caagtatcac gactagtgtg gctatcaatg gtgtagcacc
    27301 atacaaatca gttatttctc aaggatttgc tttagatggt gaaggccgta agatgagtaa
    27361 atctttagga aacatcattg caccagaaaa agtgatcaaa caatttggtg cagatatcct
    27421 tcgcttatgg gtatctagtg tagattatga agcagatgtt cgtgtttcta tggatatctt
    27481 aagtcaagtg gcagaagtat atcgtaaaat cagaaatacg atgcgtttct tattagcgaa
    27541 tacagaagat ttcgatccta aaaaagatac tgtttcgtac aatgatttac gttctgtaga
    27601 caaatatatg accgttcgat tgaaccaagt aatcaaagaa atccgagaag aagggtacga
    27661 caaatataac ttcatgcata tttaccgtac agttatgaac ttcttgacgg ttgatctatc
    27721 gtcattctac ctggattttg ctaaagatgt agtttatatt gaagcagaag atgattatca
    27781 acgtcgttgc atgcaaactg ttttctatca aacagctgta gctttgacaa aattattgac
    27841 tcctatcatt ccgcatacag cagaagaaat ctggtcattc ctgaaagaag aagaggagta
    27901 cgttcagtta agcgagtttc ctggttatga agagtttgca aaccaagaag aattgatgga
    27961 tacctggact gctttcatgg acttccgtga taatgtctta aaagcattgg aagaagcaag
    28021 aaacagcaaa ttgattggta aatctttgga agcaaaacta actgtttatc caaaacaaca
    28081 agttcgtgaa atgttaaaag ctttggatgc agatattgca caattattga tcgtttcacc
    28141 agactacttt gaagttatgg cagcagatga acaagtgcca gataacgcta tggtatttga
    28201 tgatgttgct attacagtgg aaaaagcaga cggggaaact tgcgatcgct gtcgccaagt
    28261 tcgtaaagat gtaggagtag atgaaaaact tcctcatcta tgcggtcgtt gtgcaaaaat
    28321 cgttgaagac ttctatcctg aagctgtagc tgaaggattc gaagaaaagt aaaagggaaa
    28381 agggggtgtg acatgttctt tgtcacaccc ccttttgtta tcaatcgttt aattttccac
    28441 gttcacgata ccagttatct ggttgccaga tccattcgaa tccgtctttt tcaagtagtt
    28501 cgaaagctgc tttaggtccc attgatccag ctttgtagtt gggaaaatcc ggttgtgttt
    28561 ggtcccaagc ttttcgaatg acatccacga tatgccaaga ttgagcgact tcttcccaat
    28621 gagagaagtt cgttccatca ccattcaacg catctaatag taatttttcg taagcttctg
    28681 ggctattttc aaccatttct gcactatttc taaattccaa tttgattggt tccgtttcaa
    28741 atccttgccc tacttctttc ccattcaagg ataaagaaaa tccttcagtt ggctgaatat
    28801 agatcgttag aacattaggt ggaagtgtcg tatcgtcaca tggttcatcg acagatgttt
    28861 tgaatacatt aacaggaact tgtttgaata cgatgttgat ccgagtccct ttttcagtca
    28921 aacgtttgcc tgtacgtaca tagaaaggaa cacctgacca acggaagtta tcgatagtga
    28981 atttgcctgc gacgaatgtt tcagtctctg attgagcatc cactttgtct tcttcgcgat
    29041 agcccttgaa ttgtacacca tccaattcac cttgagcata ttgcccgcga acgaagtttt
    29101 ctaacacttc ttcttcgtta taaatgcgga tagctttcaa tgctttgatc ttttctgtac
    29161 ggatttcttt ttcggaaaat gctactggcg gttccattgc aagtaatgct acaacctgta
    29221 aaatatgatt ttgcaccatg tctttaagtg cgccactatg atcgtagtag cccccgcgat
    29281 cttctacacc gaggttttcg ccaaaagtaa tttggatatt gtcgatatag cgattgttcc
    29341 attgagcctc aaaaatatta ttggcaaaac ggatagctga gatgttttgg atcatttctt
    29401 ttcctaaata atgatcaatc ctgaaaatat cttgttctgg gaatacttct ctgatttgct
    29461 ggttcaattc aaaagcagac tggtaatcag aaccgaacgg tttttctatg atcaaacgat
    29521 tgaagccatt ttttgtcata atattttgtg atttcaaatg agaaacgatc gttccaaaaa
    29581 attgaggtgc catagccaaa taatagacac ggttaccgtt taattgatag tgtccatcta
    29641 atcggtcaga caattctttt aatgtctgat aatgcgttga atcatttaca tcatgggatt
    29701 gatagtaaaa atggcttgca aaggtagttg cttcttcatc tgaaggattc aatgcacgga
    29761 ttgtttcttt gacaacttca cggtagtgct catcgctcca gggacggcga gcggttccga
    29821 ttacagcaaa atgctcgcct aaatttcctt ttctgtaaag gcggaacaaa gacgggtaca
    29881 atttacgctg ggctaggtca cctgtaccgc cgaatatcgt aaacaataca tttttttcat
    29941 tagacatgtg gatacactcc tttatatttt atcgacaaaa atcgtgctgg ccgccttaaa
    30001 gctgacgggg attttctttc tatcattttg gatagtgatc ggtccttcat aagctgcgat
    30061 gtcaacgatc tcatattctt cgttgatatt caggtcaata gtgactaagt aatccaacaa
    30121 ttctttttca tcaagcactc gtgcgatacg cacttttgta ccgacaggat aggactccaa
    30181 tgtttgtcgg cgttcttcat ggacaacttc ctctttttta ggaatcgatc ctccatgagg
    30241 acaatgttca ggatgattca aatagttttc taaatgatct gcaagatgtt ccgaagtcac
    30301 atgttccaat acttcagcat catcatggac ttcattccag ctatagtcta aatgttcaac
    30361 taaaaaaact tcccataagc gatgtttgcg aatcagactg ctagcttttt gcagtccgct
    30421 tgcagtcaat tgtacacctt gataagggga atgttctaca agctcttctt tcacaagttt
    30481 agaaatcatt tcactgacag atgcagctga gacatctaga ctagatacga tttgtttatt
    30541 gttgactttc gttgtatctc cacctagttc taaaatgatt ttcaaataat cttctcgatt
    30601 tggtgtcata ttcgatcatc ctttcatggt aaaaaaccat cattagtaag tgtaacaaaa
    30661 aaaacgttaa ttgactattt ttctaaataa aaaacaaagg atttttagac gtttttacaa
    30721 caaaaaacga aagatgatag agaagactca ttctttcgtt tataaaggat atatatatac
    30781 attgggaggg atctattttt cagataacaa cttatatgaa gtgtctgcag attcgtcata
    30841 aagagtttgt tcgggataag aaaagataag ataaaaacca tactgatcac gtacgactaa
    30901 acgatattca tctatttcta ccacagatgt gccaagagac tgaccgttga tcaaaaacgt
    30961 gtaatcagat gtaatctgta attgttgttt ttgctggcta tcactaaaga accatgtacc
    31021 ttgatacttt gagagtcctt ctgacttttc cgcttgtttt ttctttttca agtaaataaa
    31081 acttgagaga acggtaatga gtgaaccaag aaaatagatc atttttttta tcaccaaatc
    31141 actcctttat gagaaaataa tcgcatagac atattctttc ttctttattt cattgtaccg
    31201 aataaaagct aaaatttctc taagactttc gactggtttt tttactgttt ttctacaaat
    31261 tatgatagga tacatagtag gaagaaaggt gacataattt gaagtatcag caagtggaaa
    31321 tagctttttt tattgaccgc ccgaatcggt ttatagcaca ttgtatgaaa aaagacggag
    31381 aaataattat tgcgcatgtc aaaaatacag gtagaggaaa agaggtcctg ataccaggcg
    31441 ctgaagtggc tattgtattt gctcctggac ctaaacggaa aaccgcctat gatctgattg
    31501 ctgtaaaaaa gaagggtgac tggtttaata tcgatagtca attgcctaat cggctggcaa
    31561 ttgatggtat tttagacggc acgatccttt taccaggatt aactggtcca tttacggtat
    31621 acaagcgtga agtaacttat cttcactcca aatttgatat ttatttagag acggtggtcg
    31681 gacaaaaagc atttgttgaa gtcaaaggta tgacacttga aaataaagcg attggtgctt
    31741 ttcctgatgc gcccacactc agaggattga aacatatagg agaattaacg tatgccgcac
    31801 aagatggtta tgccgcttat gtcttgttta ttgctcagtt cgaacatctt catctggcaa
    31861 cgatccatga agaaatgcaa ccagcactag ctgatatggt tcgacatgcg cagcaaagcg
    31921 gagttcaaat cctcgcctat aattgtcaag tatcggcaga tgaggtaact gtcaagcagg
    31981 ccattccatt tgatgtagat atcccttttg aagatcctat acaatagaaa gaaggtaaaa
    32041 taatgatcaa cttaggtatt atcggaacaa attggattac tcatcaattt gtccaagcag
    32101 ctttaagcac aaaaaaatat gaattgacag cggtatattc tagacatttg gaaaaagcac
    32161 aggaatttgg ttctcattat gaaggggata tcgaattcgc aacagatttg aagacttttt
    32221 tcaatattac tcatatggac accatttata ttgcttctcc gaatagtctt cactttagtc
    32281 aagctaagca agcaattttg gcaggaaaaa atgtgatagt tgaaaagcca gctttttcga
    32341 cgccaaaaga aatggatgaa atcatccatt tggcaaatga acacaatgtg tatttttttg
    32401 aagcagcaag aaatatccat gaatcaggtt ttaaaaagat tgccgattac ttaccattaa
    32461 aagacgagat aatcggtgca aatttcagct atatgaaata ttcttcgcgc tatgaccaag
    32521 tattagatgg agaagaaccg aatatcttct cgactcattt ctcaggtggt gcattgatgg
    32581 atttaggtgt ttaccttgta tatgcagctg ttgcttggtt tggaatgcca aaagaagtcc
    32641 attatttccc aagaaagatt tctactggtg tagatggtct tggatggggg atcttacgtt
    32701 atgagaattt tgatgtatcg atccagccag gtaaaatcgg tgattcttat ttaccgtcag
    32761 agatttattt tgactcagga acgctagtga tgaatggagt caatgcaatt gaacaagcac
    32821 aatttcatga tcgcgcgcat aaagaaatca ttgatttggc ggtaacagct gcagaaaatc
    32881 caatggcaga tgaagcagag gattttgcca atgtcatggc tcatccgaaa gatccagctt
    32941 ggggattgtt gtatgaagag tggatcgagc tagctagaaa tgtgaaccaa gtgatttatg
    33001 atttaagaaa aaatggcggc atcaaattcg atgcagataa tgaggaagat ttttaatgaa
    33061 cgaatttgta caagtatttc ccgaaaattg gcagaaaaaa tatgaggata aagggtttat
    33121 ctcaccttct cttattcaac gagcagtttt tcagccacta agtaataaac aaagtatagt
    33181 agcggtttct cctacaggtt caggtaaaac actggcttat ctttggcctc tactactaaa
    33241 cgtggaacca ggtgaagcaa gtgcgttggt catttttgct tcttctcaag aactagctat
    33301 tcaagtagca gatgttgcac gtgaatgggg aaaagacaaa gagttgaaag tccaatcttt
    33361 ggtgggcgga gccaacgtca aacgtcagat tgagggtttg aaggaaaaac cagaaatctt
    33421 gattggtaca ccaggaagaa tattggaatt gatgaaagca aaaaaaataa aagcacatca
    33481 agtgaaaaca atggtatttg atgaagcaga ccagctattt gacgctggga acagccaaat
    33541 cattgatcaa attcttcatc aagctccaac agagtatcag ctagcttttt tttcagcgac
    33601 agcagatcgt tcccttgaaa gcattgaaaa aatcactggg aaaacgtttg agacaattga
    33661 cgtgacagca gaggacgatt ctcgaaaagg actgagacat tactttttac gtgtaccgat
    33721 acgaaagaaa gatgactact tgagacgtct cacgcatata gaagattttc atggtctgat
    33781 tttctttaat caactgactg aattaggcgt aatggaagaa aaattgctgt atagaggtgt
    33841 accggtagga agtctggcct ctgatcagaa caagctactc cgaaaagcag cgctagaaca
    33901 attcaaaaag aaaatggtca gagcactttt gaccacagat gtggcggcta gaggtttaga
    33961 tattacaggt cttccttatg tagtgaatgc agaagttccg ttaagtgaag aagcttattt
    34021 gcatcgttcg ggaagaactg gtcgaatggg taatgaagga atagtaatta cactcgtaca
    34081 agataataat ctaagagatt taaaacggct tttacgtcca tcaaatataa cacttgaaga
    34141 gattttttta tacggaggta agctgcaaac tgagcagcca accaaagaag atgcagctcc
    34201 tgcagtaaat aaaaagtatg cgaaaacttt ttctggaaag gagcagaacc cagaatataa
    34261 caaagaaaaa ataagaaaaa aagttaaaaa taaatcaaaa aaagagagaa ataaaggagc
    34321 aaggcgcaaa taagcgcctt tttatgctat gattatgaaa acgggaacaa aattggttgc
    34381 cagaggaggg gttatgtgaa agaacgtcaa gaagagatct tgcagctatt gacagaaaac
    34441 caacaaggat tgacagcaag tgatgtagct gagcgcttaa ctatcgatcg aagcaatgca
    34501 agccgatatt taagtgaact atacaaggcc catcatattg taaaaaccgc tggccgacca
    34561 gtcgtttact ctttgccaac ggaaaagtca aaatctgatg aagtgcatgt cgactcttct
    34621 acgcaagtga cttttgaaac actcgttgga gaaaatgatt ctttaaaagt cagcatacag
    34681 caggcaaaag ctgcgattct ttatccaccg cgggggttgc atacgattat ttttggagaa
    34741 actggtacag ggaagtcgat gtttgctgaa tgtatgtatc attttgcaat cgattcagag
    34801 atgctttctg cagatgcgcc atttgtttct ttcaattgtg cggattatgc tcaaaatccg
    34861 caactgctct ttggacacat ctttgggatc aaaaaagggg cttacacagg agcagctcaa
    34921 gatagtcccg gtctaatagc caaagcagac ggcggtatat tgtttttaga tgagatccac
    34981 cgtttaccgc cggaagggca ggaaatgctg tttactttca ttgataaagg tatttaccgt
    35041 ccgcttggag aaagtgcaca agtacatgaa gcatctgttc agatcattgg ggcgacgact
    35101 gaatcttcag aaagtttctt atctacgttc aatcgacgga ttcctatggc aatcaccttg
    35161 ccgaatcttg cttcacgttc actggatgag cgttatgaaa tcatctctct ttttatcaaa
    35221 caagaagcga atcgtttgaa tcaacgtatc gatatcgaaa aagaagcgat tcttgccttc
    35281 atgctttatg atgcggaagg aaatatcggt caagtgaaac gagacttgaa actagtctgt
    35341 gcaaaatctt ttttacatta ccgtactcat catgagaaaa agctaatcat tcgaaaagaa
    35401 gaactttccc ttcaagtaca aaaaggatta ctgaaaatca aggaaatacc ggaacgtctc
    35461 gaccgattca tggacagcaa aagtcagtat ttgacatttg aaccaggatt tgctgatgtt
    35521 gtctggtcac aagatccaga acgaaacatg caggtctata acgatattga ggaaaaagtc
    35581 acttctcttt ccgaaaccgg tgtggagaac attgatctag agaccctcat ttcaaaagat
    35641 gtggatgctt atttccagac atatgtcaaa gaattgacta aaagttcgat tcccaaagat
    35701 cttttgccag atgaaatctg gcggttaacc aatcaattgt atgatgtggc tgaaaaacgg
    35761 ttagaccgta tttataatga aaaagctcgt tttgcttttg ctttgcatct gcaaagtaca
    35821 ttggatcgtg tgagagaagg tcatatgatc gtccacccag atttgaataa cgtacggaaa
    35881 aattttaaga gggaattcca ggtagcaatc gatctttcaa cgatcattga agaagaagaa
    35941 caagttgaga ttccttttga tgaaatcggc tttattagta tgtttctttc tatcaatcta
    36001 ggagaaagtg agcctgttaa agaaaacaaa gtagatgttc tcatattgat gcacggcaga
    36061 cagactgctt caagcatgtt ggagatggca caagaactat taggaacaaa agtagggaaa
    36121 gcttttaata tgccactggt tatggaagtt caaacaatgt atgaagaagt gttacaatat
    36181 gtcaaaaact atcaagagca tttgacgaat ggcctactct tgttaacaga tatgggatca
    36241 ttaaacacct tcagtgattt gatttatgaa gagactggca ttcggacgaa agctatcacg
    36301 atgacaagta ccatgattgt gatagaagcg atacggatgg caagtgttgg aagaagctta
    36361 gaagatattt atcaaaatat ccagctttcc tttgaatcga tcgtacgtga tcagttcaaa
    36421 gcggaaaagt ctgaaacttt acagcgtaaa aaagccgtaa tcgtgacatg ctttactggt
    36481 gaaggagtag ctgccaaatt atatcagcgt attatgccag tgattaatca aaataaggta
    36541 gaactgatcc agatgcagtt tttagaacgt gaatcattca aaaaacacat cgatgcctta
    36601 ttggaagaat atgagatcaa agcaattgcg ggaacagttg atgttgatta tcaaaatatc
    36661 ccattttttt cagcctatga cgtttttgat gatgaaaaac taaatatttt aaagcgtata
    36721 gctgatgatg agatcccgac agctaaaatt gtcgattcac ttgctggatc attaagcgat
    36781 gtcttttcta ttgatgaact gatccgttca ttgcaaaaga cagttcagca gatccagacc
    36841 gatttgtcga ttatcgtgga acctggagta gatactggaa tcgttatcca tttagccttc
    36901 atgttagacg gattgatcaa acaagaaagc catcggcaat ttgagaaact gtctattttc
    36961 tcaaaaattt atcgtttgga aatggatatc gttcgggcaa acttgatggc actagaacga
    37021 aaatatcagg taaaaattcc agaagatgat atcgcatttc ttacacaaat gtttcttgaa
    37081 aataaaataa aaaaatgaac acactaatca tactgtttac acactaaaca tctgtgtgcg
    37141 cactataaaa aagagctaaa aagcatgaaa acgctatttc agctcttttt tttattggca
    37201 tgcttcttgc gttatataaa agtgtaaaaa aataaaagga gtgttaaaaa tggcaaaaaa
    37261 aacaatcatg ttagtttgtt cagcagggat gagtacgagt ttattggtca caaaaatgca
    37321 aaaagcagca gaagaaaaag ggatggaagc cgatattttt gcggtctctg cctcagatgc
    37381 agataataat ttagaatcta agaatgtcga tgtattatta ttaggtcctc aagtacgttt
    37441 catgaaagct caatttgaac aaaaacttgc gcctaaaggt attccattag atgtcatcaa
    37501 tatgcaagac tatggcatga tgaacggaga aaaagttctt gcacaagcag aaaatctaat
    37561 gaaataaatc aagtatgcag ttgggggtat aacaacgtgg aagatcaaca aaatctagaa
    37621 gccatcatgg ggttaatcat gtttggcggt aatgcaaaga gcgatgcaat ggaagcgatt
    37681 gcggctgcta aaatcggtga ttttgagtta gcggatagga aaatcgctga tgcagaagaa
    37741 tcacttatac aagcgcatca tgcacaaaca gacatgttga cacaagaagc acaaggaaat
    37801 catatgcaag tgacattatt gactgttcac agtcaagatc acttaatgac atctattgcg
    37861 tttactgatt tagcaaaaga aatcatcgaa ctttatcgtc gcgtatacag cgaataaata
    37921 caacttcttt tttgaacgga agaagaaaaa tttttgaacg gattttttat tttgttataa
    37981 ggagaactct ctttatataa aaaacttata ggaggaagta aaaaatgaac ggactatcaa
    38041 gttggatgga gaaatacatc ctgccggttg ccgcaaaaat cggagcgcaa aaacatttag
    38101 ttgctttgcg tgatgctttt atcggcacac tgcccgcaac aatggcggga tcagtcgctg
    38161 taatgatcaa tgcaattatt cgtgatttac cgcaacagtt taacagtacc tatgctgaca
    38221 gtttagcagc agacaaagga atctttgccg tattgaatgt catcatcgga atcaatggtt
    38281 ttgtctggaa cggtacacta gcaatcgccg gattgatctt tgccttttct tgggggtata
    38341 atcttgcccg agcttatgga gtaaatgatc tagctggggg gatcgttggt ttagctacct
    38401 tgattcaagg tatttccttt ggtccagcat tatcagcagc attagatgtg aatgttcctt
    38461 caaatgtcgc agatgcaatc aatagttctg agattggtgc aacgattgca gatggcacac
    38521 tgtcagttgg attatggggt tggctaaaac tagatcattt gaatggaaac gcttatttta
    38581 cagttatgat catgggagct atttcagtca ttattttttg taaattgatg cttgccaaca
    38641 ttacgatcaa aatgcctgat tcagtaccac cagcagtatc taaagcattt gcagccattt
    38701 taccagcgac gatcgcttta tacgtcattg ctattatcaa ttatatcgta ggtcgtgtag
    38761 ctgatgggca actgatcatt gatttggtgc aaaaatatat tgcagaacca ttcctaggat
    38821 tatctcaagg gttgggagca gtattgatcg tcacagtatt tgttcaagta ttctggttct
    38881 ttggtattca tggaccaaat gtattggcac cagtgctaga gggaatctgg ggacaagcac
    38941 aactaatcaa tattgatatt ttccaaaaag gttataacgg aaaaactggt acaccagccg
    39001 tgttagatgc aatcgaagat ggaaaagctt atatgtgggt ccgcggatcg ttcgatgctt
    39061 ttgcatggtt tggcggatca ggcggaacga tcgtcttact gatagctatc ttgattttct
    39121 ctaaacgtgc agattatcta acagtagcta aattatccat tgggcctggt atcttcaata
    39181 tcaacgaacc agtgatgttt ggtctgccaa tcgtattgaa tgcaatcatg tttatcccat
    39241 tcttgattgc tccagtagta gcaacagcaa ttggatggac agctacttat cttggcctag
    39301 tcgctccggt atcgcagcaa gtcacatggg tagtaccgcc gttcttattg tcattcttag
    39361 caacaggagc ggattggcgg gcaccgatcg taacattagt atgtatggtc gtgactttct
    39421 tgatttgggc accatttgtc atctctgcaa acaaaatgga ccctgccgca ggcgaacaac
    39481 aataagatga acgtgaggag cgggacgctt taagtttcgt tcctcataat gaattttcga
    39541 ggaggaactg aaatgattac aattgccagt gcaatgcaac aagatgaaat caaagaattg
    39601 ttagaaggat acaatgaaga aggaatgact tttacattta aagaaaaaca aggaatcaag
    39661 ctgttctttg aaacaagtgc agaagatccg gaagttgcgg ctaaaaaagc aaaggaacta
    39721 atcaaagcac agccttgggg aacagtcctt tattttcaat caacagctgt ttagaaaatg
    39781 gtttaaaaag ataggatggt tgagatgata aaaaacggtt tatttttatg tagtattggt
    39841 tctttgatcg tgttgtatag tttgaatcca cattcgcaaa ggtatgattt gtatagtatg
    39901 gtcacaggtc tttttctaac aggattagga atctatttct tcatgaaggg gaaaaaaaga
    39961 gaagaagcaa aggagaaaaa taactaatgg aaatcacgaa aaaattaaat attccagcat
    40021 cgttttttta cgataaagtt atggactctg tccttttcga tgtacgtaaa gcgaccggca
    40081 aatcagtaac tagaaaacag ctgaaaaatt tggagtacgt caaacaattc tcgcaaaata
    40141 accgggctcg cataaaaatc gaagaagtca ttgaaaacca gtcttataag tttcgtacat
    40201 cgacaacgaa gaatgaattc gtcgttcatt atcaaatcaa accattggac gattcgacct
    40261 gtgaggttca ttacacagaa aaaatggagt cttatggcat gcttcaaaag ttgaatgata
    40321 tgcttttagg aacaatcttg atgtatttta aacggagacg tttcaaaaaa atgctagata
    40381 tgatcgaagc aacgtattaa atcaatgaaa catggtacta tttagttatt gaaaaatgaa
    40441 gtaagctaga aggaattttt cataggaaga tagatctttc ctgcgaatcc tcactagctt
    40501 ttttgtagga aggggcagca aaatgacaaa caaagtaaga gagctaggtg tatcttcgat
    40561 tgaggaaatg tttgatttag ccacttatgc atttaatggg gcagatacac ctgaaaggcg
    40621 agaacgtttt cgtacacttg cggagaattc ttggaattac ggattttttg atgaggagga
    40681 tcggctaaca agtcaagtca tggcgacccc ttttccagtg aatttttacg gacaagagta
    40741 cctaatggcc ggcattggct atgttgcttc ttatccagaa gcaaggggac aaggcgggat
    40801 caatcagatc atggaaaaga tccttgaaga ttgccgtgat cgtaaagtaa gcttatctta
    40861 tctggcgcca ttctcctatc ccttctatcg gcgctatggc tatgaacaat catttgataa
    40921 aatcagttat caactaaaca gtcgtgatgt gccgtttatc aaaaaaaaat ctggaaaaat
    40981 aaagcgtatg gattttgaag atgcaaaagc tgcaatcaga agaatttatg aacagatgcc
    41041 ggaaaaccaa cgagctggtc tgaagcgcga agactggtgg tatacgtata aattcaagca
    41101 aaaaggcaat taccagtttg ctttgcattt tgatgataat gaggaagcaa cgggatatat
    41161 cgtttatcag cttgcaacgt cttcctttat tattgcggag tggggatttt tggatcatga
    41221 tgctttttgc agccttgttc gcttcgtaag ttctcacaat ggtgcttttg aaacatttga
    41281 atattcatgc ggctacggcg gtaatgatca aaattatcta ttagaaaatc catttgcttc
    41341 tgtaatgatc acaccttata tgatggcgag gatcgtagat atttctttgt ttttggaaaa
    41401 ttatccattt aaaaagagac aagctgcatt tgccctacaa atagaagaag atcattatgc
    41461 gaaatggaat gaaggaatcg tggaagtaac aatcgaaaat ggaaaaaatc atctctcaaa
    41521 agtagaacaa acaaaactcc caatcgttcg aggaagtatc caacatatca cgcagctatt
    41581 attagggtat cgtaaagtgg aagagttggt attccaagaa agaatacagg tgacaaaaga
    41641 atatgaagaa acgttaagct caatcttgcc acagcaacag ccaattctta atgactattt
    41701 ttgataaaaa acgggtgatg acaatatttt tgtcaccacc cttttgtttt tcatttggca
    41761 tttgaaggaa aatgttaaag taaatcctct tttttcgtaa atttgaattt tttgttttct
    41821 tttgttttag ggttttcttt tttgtggtct tggattgctt ctttcgtaat cttttcccga
    41881 ttttttttag atgaatctaa gcgttctgcg tatacttttt cttttggctt atcattcatt
    41941 ggaaaggacc tccctacatt atttcattct acctctagtt tatcatctgt taaggactag
    42001 tggaaacgtt ttgcctaact tataagctga tttttttagc agataagctt gcaagtaaaa
    42061 aacaaacaag ctacaattga tttattgaaa ttgttcctgt agctcagtag gatagagcaa
    42121 tcgcctccta agcgataggt cgctggttca attccagtca ggaacgtaca aataacccct
    42181 gtatcctttg tgatacaggg gtttgttttg tgtttgtgcc aaaaatgtgc caaactagtt
    42241 atcattttta tataaactat cgaacacagc attgattttt tctttatcct gatcttcaag
    42301 ctctcgaaca atatgaagat aagtagacat tgtagtttct aaactgctat gtcctaaacg
    42361 tttcgatacg ctaagtatat ttactccttg ataaagtaaa atagatgcat gtgtatgcct
    42421 tagtccatgc aaagtaaatt gcttttcaat ccctaattct tttaattttt tgcgcaaata
    42481 tttgctgacg gcgtttgatg aaactaatcc attttttaag ttgaaaaaaa caaaattatg
    42541 agggttttta acttcaaaat tctcgaataa ttctttttga ttgattttaa acttttttaa
    42601 gagatcaatc gtatgtttat caatggaaat tttcctattt gaagtttcgt tttttgtttt
    42661 tccccattca tttaatttat aattccatgt tttattaata gtgatgattt gatcttcgaa
    42721 atcgatatct tcccatgtta atcctagaag ttcagcaaat cgcattccag tagcaccagc
    42781 aactaacacg agcataggag aagagtactg cgcagaaagg ttttcttcta cgagtttcat
    42841 taaagattta aattgatcat aatctaaata tttatcttct tttggcttca atgaatcttt
    42901 tcctttgatt actgcttttc tagttggatc aaacggaatt aatccttctt ctacggcatc
    42961 tttcaacgat gctctaatat ggttattaaa cttaataacg gtggatttta catggtcttt
    43021 cgcatactta tttaaaaagc gttgatatcc aactctatct aaatcagaaa tcaataccgc
    43081 tggcatatat ttctttatgt tcattaacgt atcttcgtat tttctataag tgataggaga
    43141 tactgttcct tctttataaa gttgcatcca atcctcaaag taatctgata ataatagatt
    43201 tttgcgatcc cctttaaggc ctttgttcaa gttatattct aattcgttgg cggcatcttt
    43261 tgcttcgcct tttgtcttaa atcctgattt tcttatcttc gtgtattttc cgtcatcttt
    43321 tttgtatgaa atttcgtatt gccaactatt tccacgtttg actaatcttg ccataattga
    43381 atcagctctc tttctctgat acaataggaa ctataaagaa gcctactgta taggtttgtt
    43441 ttttcataga acacgcttgc tttggacggt gggcgtgttt ttatatcaat tagttatgaa
    43501 gagcaattga aactgtctct gttccaaagt aacctgtttg tgcttgcaat acattatttt
    43561 gtgcatttgc ttgggcttca gagatatcaa aaactatttt accagtttgt tgcatatcag
    43621 gatttaagct ttccataaag aaagagttag tgatattacc attttcatct tgatttgccg
    43681 acatagatgc agccgaatca gcttcgaaag tttttccatc tgcttttaat ttgaagaatg
    43741 agctatcaac agtcacagcc ttatcacctg cattttttac agataagtca actactaaga
    43801 aagtgtcttt tgcgttagta ggaagtacag atgggccaac ttgttttact acttcaacgg
    43861 agtttacttt gtattccatt ttccctacag caacgtcatc accgatttta taagtctttt
    43921 cttcttttgc tgtagtttct tttgtagttt ctttattagt acttgttgat gttgcagtag
    43981 ctgaatcttt gttttctccg ccacttagag caccaccaat accaaaaaac aaaagtaata
    44041 ttaataccca aaaccacact tttttgtaaa aaggtttact aactttatac attttcccat
    44101 cttgacccat aacttttttt gccatttaaa tattcctcat ttcttgttat aatatatttg
    44161 tgatctcaga aatgaggtat gagtccgtgt tgcagcacgg gcttttttta ctgtgcataa
    44221 gagtattttt tcttgaaata agactggcaa acattaaaac attctgttct taacttatta
    44281 ttgatagaat agaactccat gaaattttct aatttgaact gagattcatc agtcagttcg
    44341 ttctcaataa agatatttag taaaatcata attgcgattc tatcagcttc agtttcgaat
    44401 tttgaatgaa aagttgtaga gttatcgtac agtactgaat attcaaaatg tgaagcaatg
    44461 aaatgaccga gctcgtgggc taaatgaaaa gcttcagaac tgtcttcgtg tagtttttca
    44521 ttcagaaata ctattcttgg ttttggataa taaaaacctg gttcttccat ttccatatag
    44581 attaacttta aattatactc actcagcatt tctttcaact ttaaatacat acaacccatc
    44641 actccaacta ttcattttcc tctaaagctt tagcaattgc aatcgcttta cgcattgtct
    44701 ccttagatat ttcttttcca tcaaaagaaa aaacagtatc gttttctgat aaatccacat
    44761 gtttaggggt ttctctttct tctctaccta gaaggtagtc tacagagact ccaaagtaat
    44821 cagctaaccg agctaactca atagaattag gagtttgtct tttccaactt cctaaatatc
    44881 cattcgaata cccgaaattc atttcgagct gtctaatgga taatcctttt tcttttgcta
    44941 actcctttat tatttcgtaa gtattcattg ataaatcaac ctttctgaat gcttacaaaa
    45001 aaagtttaga taaataatgt aaaaatagtt gacttatata cattattaat ctatactatg
    45061 ttttgtaaac aagtttaaca actaaaaaga caacaaaaaa tactattgat taataaatgc
    45121 caaccgccaa gaaagctttt taaatcaaag tttatatgtc ttatttaact atgcatttag
    45181 tatagattat taatctaata aagtcaacgc agttttttaa aaagttgtta aatttgttta
    45241 cgaatataaa agaaaggaga gaagaatatg gaaaaaacag tctcggcaaa aatcgaagat
    45301 ttgaaattag acatactgaa acaagcaaaa gtggcgatgg aacacgcggt agataaagaa
    45361 gactctgcca tggttgcagc catagcagag attttagctc acgtttagtc atcgttttta
    45421 cggtcttctg gaagctgacc atagataaca gcataatgaa tatctaaata agcttgaaca
    45481 atttcttttg gcgaaattga ttcgccttta gtagatactt gtgattcgtg atttagccat
    45541 gcaacaacta aatccgcagc gatttcaaac ggcgttacac tttgtgtcag ttgccataaa
    45601 gaatttcaca atatgtatgg tcgtggtaat aataccaaag agcaatttga ggaatttatg
    45661 gaaattaaaa aatacaaaaa atcatttatt aaaggaggct aaacaatgaa caaggaaatg
    45721 agccaaacaa tcaaggtaca aaaaatgata gatgatctaa cacatggaat tgatagccaa
    45781 gccgataaaa tcatcaagga attgcaagga caaaaagtta aggatgcaaa aatgcttctc
    45841 aagactatca actttgaaat gaatccaaca aaaagaaagc tcgccgatgt gttggaagaa
    45901 aagttggctt ccgctatcaa cgagcaagaa ttactatttg aaaccgatac ttttaacagc
    45961 tgatgtttta tagatgacag tttcatccga atctatatca tcgccgagag aaaagaatgt
    46021 tgcttttgac aaaaagccga taagacctac aagcggtctt gatgttgcaa gagtagatac
    46081 cccaatatca ccttgcaagg tattcacgta aatttgagaa gaatagaaca ttccatcttt
    46141 ttcttcgata tttgacgcat catcgtatcc aacgaccaaa gtatcggcaa atattttgat
    46201 tttttctcca tcaagcatag taattacaaa ttctggtttc ataaattttc acctccttaa
    46261 caattatttc agcctgtcac actgataagg aaattatacc aaagaaagga atgagaaata
    46321 tgaatacacc acaaattttt aatttcgagc aaaacgaagt tcgaactatt ttagtaaatg
    46381 atgaaccata ttttgtagga aaagatgtag cagacgttct aggatattcg aatccgcaaa
    46441 aagctattcg agatcatgtg gacttggagg ataagacgca gaacgattcg ttcaccgtca
    46501 atggaacagc agttgttttg attaacgagt caggccttta cagtttaatc ttaaaatcaa
    46561 aacttccctc tgccaaaaaa tttaaacgtt gggtaacgag tgaagtcctt ccaacaatta
    46621 gaaaaacagg tagctattca aacgtacctc aaagttttgc acaagcattg cgtttagcag
    46681 cggatttaga agaaaagaac caattactcg aacaacaaat tgccgagtac gaaccaaaga
    46741 ttagctactt agatacgatt ctttcatcga cagatacggt agctacttct caaattgcag
    46801 ctgattacgg aatgtcggca attgctctaa acaaattgct aaacgagtta ggtgttcagc
    46861 ataaagttag cggacaatgg atactttacc gaaaacatat gaaccaagga tacacaaaat
    46921 cgcacacaag tgagataccg aaagccgatg gcggcactaa agttgtaatg aataccaaat
    46981 ggacacagaa agggcgtcta tttatctacg aattactaaa aaaagaagga tattaccctc
    47041 aaatggattt agaggaaatt ggttagaaag gagttttagt atgactgaca ttgcagaaat
    47101 cactcaacga gatagagaaa aaatcaaaga atatgtcgaa agttcaaagt tcttaactta
    47161 caccatgctc gctgaaagat tcggcattag caaaagctac ttatctttaa ttttaaacgg
    47221 taaaaagact tctgcagaag caaatagaat catagattcg attattacta tgtacgaatt
    47281 gtaaagggag gaacagctaa tgcaatatct agaagcgaaa atcccagttc cagaaggcta
    47341 tgtaattatc tcccaagtgg attatgagga gttaaaaaaa gctgatgata ctggtagatg
    47401 gatgacgttg ccagaagtgc tagaacggat taacagaaaa tatgattggt ttacttctag
    47461 agttttaaag aacccaagat atagaaatat tatcgatatc gaaaaaaaca aaaatggatt
    47521 tgtctattat ccagttgaag gaagagacac atatctattt ttaagaagta aaacacttga
    47581 atttttagaa acaaattttt cggaaatctt aaggaggcaa gcggatggga aaatttaata
    47641 gagcgctagt attcagcgca ccgctaatca tctacgcttt aggactttgg ggaagcagac
    47701 aagcgttgat aggaacgatt gtttacatgg tttggatttt tatggggctg gatgaagctg
    47761 agtacaaaac aaaaaagcca gtcgggaggg actgactaat gaaaaaaagt ttaatgacta
    47821 taaacgaaaa acaattgaaa gaaaaattta atgatctcat caaagaattt gtaaaagaaa
    47881 ctggagaatt tcctaatcaa attcatctag ttgcggaggg gcatagccgg tatcaagctg
    47941 tgaaatttga gatgaagaaa caaatctatt gaatttagca gtacctctac ttgtaagaac
    48001 atttattgta atgttttttt cgactaaaga ttcatcgtat ctaaaaacca acgtagtaat
    48061 ttttgactca tacggtggaa tggtaatagg taatgacaac ggaggaaatt ttgatagatc
    48121 agcacctttt tttaaagatt taccagaata cgcacgtata ccatgtggaa gttcgtcata
    48181 cattgatttt accgttacag aataattatc accaatcatt gtaaaagcat ttaaaggatc
    48241 aggaattcca gaaatcgaaa actcaataat tgtaacaggt aaagaactgt tatttgttaa
    48301 caatacagaa tctataatcc tgaatttatt ttgatggtaa acatctggtt tggaatcact
    48361 ttctctatct atcaaccatt cttcgcattc agcgaagaaa tccaatttta aattaaattt
    48421 tttatttttt ctatctgtat aggagatata aaaagcaaat gttgaaaata ctagagacgt
    48481 aataggcaaa ataaaatctt taatggtttt aacaatgtta ttccagtcaa attgtttaat
    48541 aaagtcaata agactcaatt tataccacca ccatttttta tctacattat atcaaagagg
    48601 agagaagaaa taatgcaaga attagtaatt ttgaaaaata aagaagctgt gactacgagc
    48661 atacaagtgg cagaaagctt tgagaaaaaa catcaacatg ttttaagaga tattgatgca
    48721 ctaaaaaaag atgtgtccaa ttttggacag atgttttctg aaggtaatga gccagattcc
    48781 tacggaagaa atcgacgagt tttcttcatt agcagagatg gatttttctt gttagctatg
    48841 ggatttacag gaaagaaagc tatttacttc aaacaaaaat acattgaagc attcaacgaa
    48901 atggaagatg ttattcgcaa gaatactgtt cctcaaacaa ttgaagacat gatgatctac
    48961 caactagaag aaatgaaaga tgttaaaaaa gatgtctcca tgcttaaaga tactatgcga
    49021 attagcggac aacaagagtt tgaaattaag caaaaaggaa atacgaaagt tatggaagtt
    49081 ctagggggaa aagaaagccg agcttatgaa gaaatcagca aaaaagtatt ctcaaaattt
    49141 tggtctgaat ttaaacgtac cttttcaatc ccaagatatg gcgagttacc tcgtaagaga
    49201 ttcgatgatg ctgtttcatt tattgaaatg tggttaccag aaactgcgat tcgcatggaa
    49261 atcgatcaac tgaacagaca acaaagactt tttggtgatg aaaatgaata gagctgaagc
    49321 gctaagaatc gggacggtaa ttgctaatcg ctggtggaga cacaataaac caatcatcct
    49381 aagccaacaa catattgaca agcaaaaagc atggcaacaa ataaaaaagt gactccgccg
    49441 gcaagcaaag agtcacaaag aaaacacatc ataaggagat tttagcatat ggaaaaagaa
    49501 ctttccactc tagatcaata tttgactgat tctgaatggg gcaagtcgaa tatcaaggaa
    49561 acaaataatc gaaaaatcag acgaaatctt ttgacggatg aagaactagc atgtgatcaa
    49621 gacgatttgg gaaattttgt gagtatttgg gatcatgtct atcttatcca tttatcgagg
    49681 aagtccaaaa aacctgaata tatctatgtc atcgaagatg gcttgattga tgcgctagaa
    49741 gagtatgaca gagataactt gattgatatc tcttattacg gatcaggtaa gaaatacatt
    49801 gctgaaatgg aggcagaatt tgatgagtga aaacaaaggg acaacgactt ttgaaaaact
    49861 ttttagtcgc aagttaaata aaatactcaa gaaaaaagga aattttgatt atttatcttg
    49921 ggctcacgca tgggagatta tgaaaaagaa tgatccacag gcaacggtaa ctattaatga
    49981 gtataaacac tacagagttg tttctggaac tcatcaagac tttcttgttg aggaatataa
    50041 accttttctt atggatgaaa ctgggactta tgtatctgtc tcagtaacgg ttaaaggaca
    50101 cacggaaacc gaattatttc ctgttttaga ttatcgaaac caaccagttg ttaaaccaaa
    50161 tgcaatgcaa atcaataact cattgaagcg atgctttgtg aaagcattgg ctctacacgg
    50221 actgggatta tatgtatttc aaggggaaga tattccaaca ccacctagaa tcgatacgaa
    50281 gaaattaaac atgctagaga cgattctaga agctttcaat gagcagatgg gtaaagatat
    50341 gaccaaaacc ttaatcgaat atgttaatga gcagacagat aaattagggc tcttagctga
    50401 taacgttgaa actattgaac agttaagcta tgagcaatgt gccttgatgg agcgagcaat
    50461 agcagctaag agaaaagaat tagataagaa gtgatatgag tgtttaaacc attaatcgat
    50521 tcatattcag cggttctgaa aaagttcaaa ggaaaagaca taggtgcaac catcaatgag
    50581 gaagtgaaca ttgatcgact aaagacgatg tatgacggct acgatggtga tcgagttatt
    50641 gaaattcgtt ttattgatcc tagacgtttc acagtacagc aacgaaactt catctatgca
    50701 ctcataggcg atattttcat cgatacaggc atgccaacgg acttctggaa ggaattcttt
    50761 tacttccgct ttgaaggtgt cacagggcgc aaaataagcc tcaaagatga atcgaatacg
    50821 actgtgagtg atgctaacgt cttagcaaat atcatcttag atttcatctt tgaacatcat
    50881 attcctttca aagaaggcta tgagatttta ccagcgaatc aagaatatta cttctacaaa
    50941 tgcatcacaa aaagagtctg ttgcatctgt ggcaaaacag gagctgatat cgatcacttt
    51001 gacaaagcgc taggaagacg aaagcgcaaa gaagttgatc attcagagta cacatttgca
    51061 gcactctgca gaatccatca cacggagaaa cacaagatag gtgtgatcaa tttcaaaaat
    51121 aaatatcaaa tcaaagggat caagttaaac caggaaacaa ttaagaaact tagaatagga
    51181 ggataaaaaa tattggctga caacaaacga tactactatt taaaactaaa agagaacttt
    51241 ttcgatagcg acgagatggt tcttttagaa agtatgccag atggatacat ttattctaat
    51301 attcttctca aactttattt aagaagtctg aaacatgaag gcaaactaat gtttaatgac
    51361 aggattccat tcaattctac aatgcttgcg actattacaa gacactctgt gggagttgta
    51421 gaaaaagcag tacaaatatt tcgtgattta caacttattg aggtattaga taacggagca
    51481 atttatatgt ctgatataca aagttttatt gggaaatctt caactgaagc tgataggaag
    51541 agaaaataca gaaaagaaat tgaggaagca aaacagaatt taataacaag tggacaaatg
    51601 tcggacaaat gtccggacaa aactacacca gagttagaga tagagttaga gaaagatata
    51661 gagttagaga aagatataga gaatgtaacg ccttcgaaaa aatcgaaggc taagcccgtc
    51721 cgtcataaat acggagagta taaaaatgtt cttttgtcag atgagcagat ggagaaactc
    51781 aaaatagaat tccctaatga ctaccaagag cgaatagaac ggctatctga gtattgtgaa
    51841 tcatctggta agacttataa aaactatttg gcaactattc gaagctgggc aagaaaagaa
    51901 aaaagtgaat ctaagaacgc aagcagtgga tacaagcgca caggaagacg agagaagctt
    51961 cctgagtggg caatcgacca agaagcctat ctcaagaaaa aagcgctaga acgagctaat
    52021 agacaatcaa aagcaccatt ctaagaggtg ggaaattgaa aatcgattat ctagaactaa
    52081 ttaatgaaat agcaaagtat aaaactggtg aggaaataga aatcctgaga gacgtatatg
    52141 atcaactcga agaagctgga atcgaaggaa ttaagaatga tcgttcgagt tggagtaagc
    52201 tcagatacta tttcgcactt tatatcgata caacacaatt aagaaattta gcatatacca
    52261 aattactatt tgttgattgt gttaaaggct tgcaaaaaca tcttaatgaa cttgagcagg
    52321 tgtaatcaga tggacctaaa gacatttaca gcacagattg aactaatgca tcaagaagct
    52381 ttaagacaaa gtgtatcgta cgaagacaag tggctcaaca cgttccacgg tggacgtgag
    52441 agcgcacttg atcaagtact caaattattg aaaggggaac gtcgggatgg ataagaaagc
    52501 ggcttggcga aaattaatgt tgctgattca agatgagaac tggcaagaag atgaagcagt
    52561 ggttgctgaa gttcagcgtc tagaaaagat tgctaacgga cgtatacgaa aaaagcctga
    52621 taagcggaaa cttcgcaaag gaaaaatcat cgttgttttg tatgaaggta acattttgat
    52681 gcaaggaacg gcaagagagt tgtctgcaga aactgaatat acgagcggaa ctattcggac
    52741 gtacgcgtgg cggaatcacg ttgataaaaa aggacacgaa tataagtatt tggagggcag
    52801 caaatgatac cgaagtttag agcgtggtac acaccattta aaggtaaaac aattggacaa
    52861 gaaatgaaat atgggcaagc aggaaggttg atcactcatg ctgaaatggc tccagataaa
    52921 tatgtgctta tgcaatccac aggactgaaa gacaagaacg gtgtggggat atttgaaggg
    52981 gatgtagttc ttgtcaatgt aagtaatggt tttgatcatt tagttaatga aaaaacggtt
    53041 gtacaagaat ccgagtttca ttctggatta gtttgtaaat cattaattag tggaatggaa
    53101 tatagagtat tcaaacacaa aaagatggat tatgaatatg aagtcatcgg aaatatttac
    53161 gagaattcag agttattgga ggaacagcga tgaataaaca ggaaacaatc aaaaagttag
    53221 aaagtattaa agcgatagga aatgatgcaa tagctgcttg ctataacgag agtatgaatg
    53281 caggtattac gttaatgaaa aaaatagacg aaccgcagaa acacgttatg ccgaagtttt
    53341 ttgacaaatg ggcaaaacaa gtcctggaaa aacgtgacaa gttttatgcc atttctctta
    53401 ttgcacgtgc aggttgggga tatggtgttg attttgaact tagcgagaac ggattatcat
    53461 cagaaaacaa agagctgttg tactggcttg ttgataaatg cagcgacaat tatcctaata
    53521 aaaagaaagc aatagaagct ttgttatacg gctacgaggt cgagaaagaa ccgttgtatc
    53581 acgtgaagct aaaaatacca ggcgtaacat actaccttat ccaaactttt ttaatgccag
    53641 gagatactca tatgtgtttc tcagcggcta cagaacgtta tggggctaaa tggaaaaata
    53701 cttttacgga atcagaaata aaagcaattg atgaaagata ctggccattt gctgtgccag
    53761 tggaagagga ggcagaagga tgaaaaaatt gcaccaactc atatctgaga aagaaagcga
    53821 actccaaaat ttagaagata gtttgggatt ggggtttcca attgttgagc aagctaaaat
    53881 gactcaaatc agtcatctac gacttgaatt ggaagattta agacaaatag aaaaatcaat
    53941 tcaactcaac gacaaccagc aaatcgtgtt tgagtggttg aaactaactg cgccaacagg
    54001 aaagccaatg caagttgtat tttggatgat gaataatgca gcatgggggc atttagacga
    54061 attaagggat cctttgatgg aattaacaga caaagagcaa ttcgaagtcc tagcagcatt
    54121 cgctcaatgg ggattagaac aggaggaagc ggaatgaaca acagacatcg cagaataacg
    54181 aaactaagaa aacaggaact gaatgtacta aaggcaaagt tcgaaaaaga atacggagtt
    54241 tctgcagaag aagcatataa agtggcaagt cagtgtgttg ctgatgcgag taatgctatt
    54301 cgtaagtttg gaatttcgat attaagtgat gatcgtaaat gggaggatat agaatgaagg
    54361 tttatgtagt aaggttcggg aataaatttt atagaagtga tgaacgctct ataggcaata
    54421 atgtgttatt ccttgtagat tttcttcaaa acgcaagatg gtttgataat ctcgaagaag
    54481 ctaaccaagt ttcacgacga cttggtggat taacgcaagt atatgaactg gtcactatcg
    54541 atcatgaggg ggtagaaaga tgaaactaaa agacggattt tacgctagta gtcatggtat
    54601 cggcggttta atgctaggta tgccgacaaa gaaccctaaa acacgtaaga aaccaaaatt
    54661 caaagtcggt gacatggttc gctgcgaagc agaggagttc atctatccgt ttcgtggata
    54721 tgtagagcat gtttataatc actcagcgat cattcgcatt gaaaacacga tggaatgtga
    54781 caagcggtta gcgaaaagca aagagaattt agcagtggtg agattggtgg atatggaagt
    54841 tataaaatct taattttgct tttttacaac tatcctacaa caatgagcgt tattccacaa
    54901 ctggaaggag aagaggcaat tgtgaacatt ttagagaata tagatattaa gcaaacaagg
    54961 aaaaatgcta gacgattact aaaaagatac agaaatttag aacgtttggt cggaccagtg
    55021 aaaatagact tttccgtgat gactgttacc aaaaatttga aattcacaat tgacagtcaa
    55081 aacgaagaaa tcattgaagc gataagtact cgagattcgg ttatcgaagc attaacacgg
    55141 ctaagtagaa tccatttcca ggtactttat tatagttatt gttttcctaa taagatgtca
    55201 atgtatcaaa taggagaaaa attaggatat tccgatagaa ctatcgagag aatgaaggca
    55261 gtagctttgg ttgaatttgc tgaggcgtat aaatcaggag aactcatttc acgtacaaaa
    55321 taaaaaaagt cggattcctc cgactatgaa taatatttcc gacataagta ttatatcata
    55381 attgggggaa tcagaggatg gtacttttcg atgtaaagaa atatgagaca ccaagcgcga
    55441 aagatgttga tatggaacgc acaaaacata atgtcgctgt gtttctttca gcatatctat
    55501 cagctagatg tagagtaggt caacctcgag aaccaaaagt gacagcatct tattccttgg
    55561 ttccaccttc tacagctgat catatatttg aagcagaaag aatgctgatc gataaagaag
    55621 aagcacagga agaatttgag tatctgcata aattgtttgt ccgaggctat tcagcgatac
    55681 agcatccgca taagcctgat gtgactgaaa ggcgcaagaa gatattctat gatcgttata
    55741 tcaatggcat gtcgatttat gtaactgccc aaagaaataa tacgagcgag gaatctgtaa
    55801 aagcagaatc aaacaaaatc atcattcaat ttgcttcttc cttagaactg gttgctttca
    55861 agtagccagt ttttacactt tttataccct tttattacca atttggtttc cattttatac
    55921 cttttttata ccaatcactt acctattcaa cgttgtatta tgatagtgtc gaaagattag
    55981 gaaacaggat cgacaaaata aaatgtaagg gaggaaatct ccctcatcgt ttaattaagc
    56041 ttcgataggc agcaacggaa atattaagaa taaggatgtg aatttcaact ccttctaaat
    56101 tgttcttatt atctatcatc cgttgctgtc tattaattta tgtattggag ggtaagaata
    56161 tgaaattatc gattgaaggt acttcaggag aaataaaaga aatcctctca gctatttgta
    56221 atagccgaga gattagcaat ttaatcaata tagataattt aaatgaatct attgttactc
    56281 caagttcccg agagaaattg cagaaatctg atcagcaaaa acaactaaaa atgggatatt
    56341 agtagttctc tcgcctatga tggtaacatc tttcagaaag atagatttga ctaccaaact
    56401 ttgctcatca tctttttcta tatcatcaat tttttttgag cgaaattctg caaaagaatc
    56461 catcatagca tcaataagag gatttacttt tacttcagca tcaaagtcta taggagttcc
    56521 gcaaattgtt gctcctccag tttgaataat aatattattg ttagaatttt tagcgaagag
    56581 ttcaatatat tcaatcatgt ctgaacggta atcatcaatt tttgccattt ttttaccacc
    56641 ttaaattatt tcagcggacc actcgctgat aaataaaatt atacgcttag tatttatttt
    56701 cacaatatta atttatcgta ataacttttg agtaaataga acaaaaaacc tgcactagtt
    56761 tccgctagta caggcagtga ctatatcggt tgataatcta gcatatatta aaaataattg
    56821 caagaaaaga aatttatagt ggtttatacc aaattatcaa agcaatgtta ttttgttgct
    56881 gtctattgtt ttttaattat tcacacgata actaaaggtg ggtgaagaga aatgattcca
    56941 ttaataattt caatttttgc gctctgtctt aatgtctata tgattggatt taaaaatggg
    57001 caaaataaaa aatagtagca gccaagaata attttatagt gtcactgtgg cggaaagggt
    57061 agacgctaag catgtgtgct aggtcaatgc ttcggcaacc atgcaaggtt cgattccttg
    57121 ccagtgactt tggcaaccga ggcatcggcg gtttaaaaat ataggggtgc gcaatttcgt
    57181 acgcgttttg tgcatcgtgt aggttgctat tacataactg gttaggttag attgagattt
    57241 gggattcggt acaaatgaat cgtcaaatgt ctcaagcaca ggatcggaaa cgtccctgcc
    57301 tgtgcattac atattagatc actcgttgag tggtcttttt attttgcaaa aaggaggtaa
    57361 caacaatgta tagaccacaa tacttagaac agaagcatga agtaatcact gtgcaaaacg
    57421 gtaacggtga gatagtacga aagtatagaa gaccaataaa gagcaataca tataaacgaa
    57481 aggaaagcaa tgaagttatt ccattgtatg gcaaaagaat agctaagtat taaataagat
    57541 tgcgaaagga gatgggacat gaccgaggaa ttctatagat ggttattaca gttgataaga
    57601 gaagatcgtt tggttaagtt ctatcagtct cctaaatggc gcaggcttag agagaaagcg
    57661 atgaaacgag atcactatga atgccaagag tgtagaagac taggtaagta tcatagagta
    57721 gagaacgttc atcatataaa ggaagtcaag gatagacctg acttagcttt agatttagat
    57781 aatcttattt gtttatgtgt tgaacatcat aatgaagttc atggcagata tcttacagca
    57841 ttagataaac aagagaagaa gatagaaagc tttgctaact tcgatgcaag tgaaaggtgg
    57901 taagtgcatg atcatcaatg acaatggcag agagtatgac acagaaaaga ttgaagagta
    57961 ttcatcttat actcaaggat taattaaacg tttgatatac gttcgctatg taggtattag
    58021 ggatctgtta tcagataact gttgcagtaa atacaaagtg aatcaagtaa gagaagcgtt
    58081 gaataaagat aataacgttg aaagaataaa aaatattttt ggatatagca ttgaagagat
    58141 taattattac attgacttcg ctgaagcttt cattccgatg gtgagataac ccccccttaa
    58201 aataaatcgc aaattttttg ggggtgatga aacggagggg gctgtcagga aaagagattt
    58261 tttcgaactt tatcatgaaa ggagggctaa aatgtttaaa aacgaattgt ctcaaaatcg
    58321 ctacagagaa aaattacgcc gctctttaat aagccaattg gaaagtcaga aaacaaatat
    58381 tgagccattc ttagataatg ttgatcgtta tatcagttta tgggaaacgg cgatatcact
    58441 ggaagaagat atatccgaga acggcattag actggagaat ggtaaaaaga atgaatcagt
    58501 agcgttgctt gtttctgtca acaaacaaat gggattgatg ttggataaac ttgccattac
    58561 tcctgaattg gtaggtgaag caaatgaatc aattcctgag ttataagcat attgaaaatt
    58621 ggttcaaagc tatagaagaa ggcactatca aggtatgcaa agagcaatta ttgctaaaaa
    58681 attatctaga agaaagagtc tttactagag aagatattta cttcgataag cagatggtag
    58741 aggattcaat caatatacca gcacaatact ttccattcga attaattccg tgggaaaaat
    58801 ttctacaatg ttttatttat ggtgttcgat ggaaaaaaga taaaacgcta gtgttcaata
    58861 gatatctttc attaatggga cgtggtaatg gtaaaactgg ttttgcttct tggaacaatt
    58921 ttttcttgct gactgcaaaa cacggtatta aaaattatga tattgatatc tatgccaata
    58981 acgaaagcca agcaaagact agttttgatg atgtatttaa agtaattaaa gatcatcctg
    59041 gtttagataa aaaagtattt aaagctacga aggaagttat tcaaaatatc gctacaaaca
    59101 gcaaacttcg ttataacacg gcaaacgcta gaacaaaaga tggtaaacga ccaggagcaa
    59161 accgctttga tgaaattcac gaaaatgaag attattcaat gataaatgta gctacttctg
    59221 gtggtggtaa aattcgagat tatagagaat tttatgatac aactaatggt catgttcgtg
    59281 gtggtccgct tgatgacatt atagaagaat caaaaatgat tctttctgga gaacttggaa
    59341 ttgacaagga tggagcagaa ttttctagtt tgtttccatt tatttgtcgc ttggataatg
    59401 ataatgaagt tgatgatccc gatatgtggg aaaaagcttg tccaactatt aattacaatg
    59461 cagatttaaa acggaaaatg tttcaagaat actctcaaat gcaacgtaat gctggtttaa
    59521 gacttacgtt catgacgaaa cgaatgaata gaccaatgga agatacgcga tttgctgtag
    59581 cttcatatga tgatgttctt catacgaaag aaaaagaatt tcctgaaaaa atggatgaag
    59641 tgataggaac agttgatttt gctgatagac gagattttgc cagcgttggt ttgttaggaa
    59701 aatatgataa agatgtgtat tttacacaac atacttttat ccacgaatca gctcttcgat
    59761 tacagaacat aaaacgagag gttatagata tttctataga tcaaggaaaa tcacagatcg
    59821 ttcatgggaa aaatatagaa gctgattata ttgtaggttg gtttcttgaa atgagtaata
    59881 aatattacat taaaaaaatc gctatggata tgtaccgtgc aaaaatattg aagcccgctt
    59941 tagaagaagc aggttttact gtagaaattg ttcgaagcgg atctgttaca catggtatgt
    60001 taaaagacct agttgatgac ctttttatta atcaacgttt attttttggt gacgatgcga
    60061 ttatgcgttg gtattgcatg aatgtatatg aagagcatat ttctaatgga aatatacgct
    60121 atgaaaaaat agaacctgaa actagaaaaa cggatggctt tttttcattc cttcatggtt
    60181 tgaatttttt agatgatatt tatgattctg ctcctgtaac agtcacaaat agttcagtag
    60241 aaaatgcagg aactggattt actcctctag tattctaact tgaaaggagg tgagaaagtg
    60301 gggatttttc aaaaggcggt aggatacttc acaaaaaaag caacggttcc tttagaagaa
    60361 tacttttgta aattgcaagt tgattttgtg tatcgaaaat ttgcaattga aacttgtatt
    60421 gatttgattg caaatgcgat gagcaaagcg gaattcaagt catatgaaga tgggaaaaat
    60481 aaaaagaatg atctttacta taggctgaat gtagctccta ataagaaaaa taatgcaaca
    60541 gaatttagaa aaaaactgat caggagatta atattctaca atgaagtatt gatcgtttct
    60601 ccgtctaata attctagcga aatatttatt gcggatagtt gggatgtcac agagtatgca
    60661 ttaaaagatg atgtgttttc tcaagtgcaa attaacaaca tagtccttga tagagaattt
    60721 ctagaaagtg atgttatcta tataaaatac gcagatcaac aaattaggca actagtcgat
    60781 gcgtattatc aagcgtatgg gaaactcatt tctagtgcca tgaatgttta caagcgttct
    60841 aacgctcgta gatacgtact gaaagggaat ttattccgag cgcaagacaa tacaacacaa
    60901 gatcaaatca ataaaatgat gacatcacaa tttaaggctt ttatggaagc tgataatgca
    60961 ggtgcggtat ttcaattaca aaatgagtac aaattagaag atttcagcgg aaactttcaa
    61021 agcaattcaa gagatataaa aaacttaata gacgacatct ttgagatgac agcagcagcg
    61081 tttcacgttc cgaaaaacct actaaaggga gacatgagtg gattatcgga tcaagtggac
    61141 gcttttttaa tgtttgaaat cataccgatt gctgaactta ttcaggatgc gtttaacgct
    61201 agtctctatg aagtagaaga atacttgtca gggaattttg tacgtgtgga tacaactatg
    61261 atcaagatta ctagcttcaa agatttggtt gacgctattg atgtaggcat tagaaatggg
    61321 gtatttacaa tcaacgaagg aagagaacgc gttggaaatg atcgctctga taaggcgatg
    61381 gcagatgaaa tatttataac taaaaacaat caacaagtat cgaaaggagg tgaggcgaat
    61441 gacgacaatg aaaacatttc tagcagtaaa gaatgaaggc gcagtaccgc aaatttttat
    61501 tcagggattt attggttcta gttggttctt tgaagggaat actgacaagg gaatcaaaaa
    61561 tattttggat agtctaggtg atcaagaaga gattgaagta gtaattaatt caaacggtgg
    61621 agacgtattt caagggattg ctattgggaa cttacttaag tcaaataaag caaaagttaa
    61681 cgttgtgatt aacggcttag ccgctagtgc tgcttcaatt atcgcaatgg ctggcgatac
    61741 tataaaaatc tacaacaatg cacaattgat gattcaccgc gcttccacat acggagaagg
    61801 aaatgtcgat gacttccgta cgattgctga ccaactggaa tcaattgata aatcggtaaa
    61861 ggcttcatat aaaacacgat tcaatggcac agatgaagca ttgcaagaac ttcttgaaaa
    61921 agaatcgttt atggatgcag aaacagcttt gagttatgga ttggtcgatg aaattatcga
    61981 tgcagaaaat agctcaggta ctgaagctaa aaaagaacaa agcgttgaag aaattttgaa
    62041 tgacgttaaa gaaaaaagag aagaaaaaat tgctgcattt acagcagcat taaataaaac
    62101 atttggacaa ggagatgtaa aataatgaca gttaaaaatt taaaaggtgt aacagctgca
    62161 agcgaccaat tgatgaaagc ttttaaagat ggtaacgaag aatcttttag cgcagctatg
    62221 gtaagcttat ctaaggaaat tcaggataaa attttagaag aagcaacagc aaaaaatcaa
    62281 gatcaattag tattaatgaa ccgtggtcag cgtgtattaa ctacacaaga aacaaaattc
    62341 tataacgaag tagtgaaaaa cgaaggtttt gcaggggttg aagaattagt gccagctact
    62401 gtatttgaac gtgtatttga agatttagaa caatctcatc cactattgca aaaaattact
    62461 tttgttaaca caactggtgt aacagaatgg attgtgtcac gtggagtcaa tccagcatgg
    62521 tggggtaaac tttgcgaagc tgttaaaaaa gttttagata atggctttga tgtaattaac
    62581 atgaagcagt tcaagctatc aggttatatt cctgtatgta aggcaatgct tgatttaggt
    62641 ccagtatggt tagatcgtta tgtccgtact gttttagtag aatcattgag aattgcatta
    62701 gaacaagcaa ttgttgatgg tactggtaaa gatatgccag tcggaatgat gcgtgacatg
    62761 agcaaacaaa ctagcggaga atatgctgaa aaaaaagcag aacctattac agctttagat
    62821 gctgtaacta tgggcggttt gatggcgcga ctatcaaaat tcaatatcga aggtgtgaat
    62881 gatccgattt atcgtaatgt gaatccttct gatgtggtcc taattgtgaa tccaactgat
    62941 tactggtcta aagtattccc agctaagact gtactaactg ctaatggaga atacgtacaa
    63001 gtattgccag taccagtttc agatttgcag tcaacggctg tgccagaagg aaaagcagtt
    63061 attggggtag cctcagatta cttcatgggt gtaggatcta cactaaaaat tgaagcttca
    63121 gatgaatacc attttgttga agacgaacgc atttatctag ctaaacaata tgcaaacggt
    63181 caacctaaac gtaacgatag tttcattgta ttagatatta gcgctttggg aactactact
    63241 acaactacaa aaccaacaac cacaacaact acaacacaag cgtaggtgat cagaatgaag
    63301 tatattcttt gtcagccggc aatcaatcgg tttaaatggg agcttgaagt ttgtttaact
    63361 aatctgaaga aactaggaat caaagatatc gtattgcttt tcagcagaca cgatgatcag
    63421 attcctattt tttttgagaa ggaatatggt gttgaagttc atgtgtacga tgatctgcgg
    63481 gacgacaaag agtatattcc ttcgattaaa ccatatttat ggtggaaata tttagaagaa
    63541 gatcattcgc gtgaggacga ccgatatttc tatatcgatt cggatgtcat tttcaataaa
    63601 agaattaatt tgcgcaaatt gccttctaaa gatgatgttt ggtattgtag cgactgctgt
    63661 agttatctaa gtcttgatta tattagaagc tgtgaaaacg gagaaaatat tctaaaagat
    63721 atggcaaaca ttgtaaatgt tacagtagaa tctttggaaa ctataaacac taattcagga
    63781 ggcgcacagt gggttattaa ccgtcctaaa gcgaattatt ggaaaaaggt ttatctggat
    63841 tctaatcggc tatatcgcta ccttagaggg caaaaaacaa atatacaaat ctggacagcc
    63901 gagatgtggg cacagctttg gaacatgatg tatttcaata ttggtcctaa agttcacgag
    63961 gaattagact tttgttttgc tactgatcca atagaaaaag ttaaagaagt aaaaatctta
    64021 cacaatgctg gagtaacaac aaacgatgaa gatttatttt tcaaagggag atacgtgact
    64081 tctacgcctt ttgatgaaga tttatcattt gtaaacaaga aaaaatgctc ttacgcatat
    64141 gttaaagcaa ttaaggcggt ggttagatga cgcctgaaca agtgactgaa gaattgctaa
    64201 tagctgtgaa ggataatatt tacgttacct ggaacgaaga agatgagtca attaaaaaga
    64261 tgatagctaa aaatgctgtt tatcttcaaa gtaaagtgag tacaacactt tctttttctc
    64321 ctgaaagctt agaatacgga ttgctaatcg aaagatgtag atacgactgg aatcgtgctt
    64381 tagatgagtt tgaacaaaat ttcgctagtg agttattagg tttcattcaa cattatgcgc
    64441 tacaagaata tattgcaggt gatgtgaatg gcgaataatc gtagactcga agaaacattc
    64501 aacgatggtt ggttaaagat tttgacgcaa actaccaaaa gaaatgaact aggaaaaaag
    64561 attggtgtag aagatacaga aatcacttct ttaaaattta gaaatctttc catgagagat
    64621 agtgatataa cagctatgga tgcgatggga tcgaaattaa ctaagaaagt aaagactcca
    64681 tttcatccaa tcgccaagaa atttaataaa gatcaatatt ttatcgtaat cgatagtatg
    64741 cgttacaacg ttatctatgc cgattacgat aatttttata tctattttta tcttgaaagt
    64801 gtgggtgaat atggtgattg ataattctaa agaaaaagaa cgtttaaata agcaaatttc
    64861 tgctatcaaa acttccttag aagagcattt tggcctcaaa ctctttcaag actccgtagg
    64921 cgaggatgag ctacctgatg attttaatta cttcattctc gaaacaggag aaatagaaat
    64981 gatcactgag ccaaaatata gcgtgggtca aaatctatat ctaactttct attcagaaaa
    65041 tagagaagat ttaacaggag actcactaga tattatttca ttgattcaaa atcgttcgat
    65101 tcgttttcag agaatggatc ccaatcattt aaaactagag aaccaagatc gctatatcga
    65161 tcaattggta tttacgttta gacgattatt gaagagtgat tgtcatggct aaaaatagtt
    65221 gggagctaaa aataaatgga catgatgaac ttcttgtgcg gatggaacgc tattcaagcg
    65281 agagcgaacg actgattaac gaagcattga aatcaaaagg ttcggctatt gcagtggata
    65341 ggattacaga aaaaattcct gtttctgaag cagatttaag aagagggcac caacacgcaa
    65401 aaaatagtcg tccacttaag actcaataca ttaatttggg tttcatcatt agacctacaa
    65461 gaaaatttga gtatttaaaa tatcctgatt tggggatagg tacttctaaa agaaatcagc
    65521 cggacgaatt tatgagaaga ggattaggtc ttgcacttga tccaattaca gaacttctga
    65581 ttcgtcaatt cgataaatta aataaatagg gggaacaaca atggctaaaa caaaaactgt
    65641 agtaacaacg ttcgataacg taagtatcaa acgaattgct tttaatttta agaacgcagg
    65701 aaatgcaatc gcaacgaatt gtaacggaca attagatggc gaaacagaaa tgcaaacggt
    65761 ggttaaaaaa tgtggagcga cagaagtaaa atcaaaatct aaaccaatca atatgacggt
    65821 aacaattact gcacatgtac cgatggaagt ttatcgacgt ttcaatgggt tgaaacaaga
    65881 tggacgtatt aaaccaggca tttactctta cggtcctgat tccgtaggcg aagatttctc
    65941 acttgctgca gagatcgtgg atgacttcga agaaaataga aagttagttg gtatgttagc
    66001 atgcacttcg aatacaggat taacattctc tattgaaaat ggtgcggatg aagtagctgc
    66061 gttagaacta gaaacaaaag ttatgcaaga tgaatttggt aaattttatc atgaagcaat
    66121 tgttgcagaa cttgaagaag acttaacaga tcaatggatg acgaatctat ctgctgatgt
    66181 gattaaaaaa acttcagttg tgacaactac ggccactcaa tcacagtaaa aaaaacggag
    66241 gtagcgaaat gaacgaagat tactcaaaaa ttgaactaaa cgatggaaca attttgaatt
    66301 tagaacctaa actgaatatc aagaaattat tgatgatcaa tagagatttt aacacagacg
    66361 agtttgcaaa aatgactgtg ggaaaaggat ccatggatat ttctgttatt caaggtgcaa
    66421 aggctgtgta tattgcttac cgccaagcga acatgactga ttatatttca ttcgatgaat
    66481 ttatcgataa atgggatttt gatatggcta ctgccagcta tatttatcaa ttgatgatgt
    66541 tcaaacaagc acgcgatgct tatcaaaaag aatttgaaaa agcaaataag gaaaaaaagc
    66601 ttcaaaagta aaaatgccaa agctcttagt tgaaacgtgg gtcgatgtct attcgatgtt
    66661 gaccgacgtt ttttctatgc cttcagattt ggttttaagc gatatctgtt tagatgacat
    66721 tttgcaaatg gcttacaaca agagtgctta tgaaggatgg aaaaactatg caataaacca
    66781 atctcagaaa aactaaagaa aggaggtaaa aaatggctaa aaagagaaca gaagcagaag
    66841 taactttcat agctaacgat gacggattga aatctacgtt aaaagaaatc agtgctgaat
    66901 taactaaaaa tagagcagaa ttaaaactag aacaagctca attacaacag actggttctg
    66961 aatcagacaa gttaggaagt aaattatctt ctttagaaaa gcagtatgaa ttacaaagcc
    67021 aaaaagttga agtaactagc caacgtttag ccaatgccaa aaaatattat ggagaaaatt
    67081 ccaccgaagt tcagaaactt gagagagaac tgattaacca acaaacagcg caacaacgtt
    67141 tgtcaaacga aattgataaa acgagtaatg cactagctca agcaaaaggc gaaatacaga
    67201 cgtacgagtc tacaatgcaa cagttggata gtgaacaaaa aaatgttcaa gctagtgctt
    67261 ctctgattga atccgaatac aaaaaatggc aagtaactgc tggtcaatca gcttctgaat
    67321 ccgagaaatt agcgaaagcc caagaatatg tttctcaaca atctgaaaat gcagagaaaa
    67381 cgatagatat cctgagacga cagttagaag ctacacagtc tgagtttggc gctacatcca
    67441 cagaagcaat gcagatggag gcgaagctta atgatgctga acgtgaattt gaagagttag
    67501 gacaagctgc taaaaatgta gatacaacta acttggacga tatcggaagc aaaatagata
    67561 tgaataattt aatggaagct tctgacgttt taagcgacat tggcgataag cttacagaat
    67621 tagggaaaca agcagtggac tcagctaaca gtgtaggtag ttcccagagt aagatacaag
    67681 ctaattttgg tttgactaaa caagaggctg aagaattaac gaatgtagcc agagacattt
    67741 attataaagg ttttggagaa tcgttagatc agtccacaga tgcattgatt ttggtaaagc
    67801 gtaatttagg cgatttaaat aatcaagatt tacaaaatat cacggaacaa gctatggtcc
    67861 tagaaaacac catgggcgct gatatggatg aaacgttacg tggtgtaaat ggcttaatgg
    67921 tcaatttcgg cttgagtgct caagatgcaa tggatttaat ggtttcgggt actcaaaacg
    67981 gtttagataa aacgcacgaa ttaggcgaca atatggcaga atatagccaa ttatggagtc
    68041 aaatgggata ttcagctgat gaaacgttcg gaatgcttca aaatggttta gatgcgggtg
    68101 cttataacct tgataaagtc aatgacttag ttaaggaaat gggaatatcg ttaacagatg
    68161 gtcgatttga gcaaaacatg gatatgttta gtgaaagtac tagaaaagct tttgaagagt
    68221 ggaaaaatgg cggaggaaca caaaaagacg ttattaattc catgattcaa gattttagca
    68281 atatggatgg tcaatacgac caattaaata aagcttctac aatttggtct gcacttggcg
    68341 aagataacgc gatgaaagtt gtccaatctt tgactgatgt taaccataca tttgatgatg
    68401 ttagtggatc tgcacaaaaa atgaatgaag attctactac tccgttacaa gaattaaacg
    68461 gaaaaatagc tgaattaaag gattcattag ctcctatagg caacacaatc atagatgcac
    68521 tcgaaccagt aattgatttt ctaggaaaga tggctgatgc gtttaataat cttccacaac
    68581 cagtacagga ttatgccgta gcgattggcg gattgactgc tgcatttact ttattaatgc
    68641 caataatagt tggcttcatg gctctaggtg gtcctactac attaataata ggagcagtta
    68701 ttactgctat tgctggagtt atagcaatta taaaaaactg gggcgcaatt actgactggt
    68761 ttaagggaat atggagtaaa ttcactgatt ggttgggtgg tacttgggaa agcataaaag
    68821 aaggtgcctc atcagtttgg gatggagtca aagaaacctg gtctggattt gtagattggg
    68881 ttcaagaaat ttggcaagga gtttctgatt ggtttggaga gttatggagc ggattagttg
    68941 aaggagcttc caacatctgg caaggagtcc aagagacttg gcaaacattc attgattggg
    69001 tttcaaatat ttggaacgga gtcaaagaag tatggtcgat tatttgggca gacattgtag
    69061 gaattgttca aataccatgg acattaataa cgtcattgat tcaagctggt attaatatta
    69121 tcgtgggtat ctttgatgta gctggacagt tattaggcgc agcttggcaa gctgtttgga
    69181 cacctatttc tgatttcctt aaaaatactt gggatactat gacacaatgg ataagcatcg
    69241 cttggaatgg aattgtaact acattccata ctatatttga tccagtagtg gcatggtgga
    69301 atggtatatg gacatctatt agtactacgg cttcaaatat ttggaactca attagtgcaa
    69361 cagcttctag tatttggaat agtatcaaga atacaatcac tagcttggta caagcagctg
    69421 ctacagtaat tcaaaatatt tggtcaactg tatctagttg gttaggtgga atttggaatt
    69481 caatcagctc tacagcatca aatatctgga gtagcgtgac tagtagtata agcaatgcta
    69541 taaacgcagc taaaagtgcc attcaaagtg tttggaatag tatatcttcg tggatcagcg
    69601 gaatttggaa cggtatcaaa aacactgctt tgaatctttg gaatggaatt acaagcacta
    69661 ttagctctaa agtaaacgat ggaaaaaatg caatttcaag cggttggtcc aatctaacag
    69721 gtattgtttc cgacatattc aataatgtta aaagcacgat agctaatata tgggaaggta
    69781 tcaaaaagac tgttagcgct ccgattgatt ggattagaga taaaatcagt agtgtctttg
    69841 ataatttgaa tatttctata ccacatattc cgttaccaca ttttaaattg agcggagaat
    69901 tcaatccatt gaaggggaaa atcccaacat tgggtgttga ttggtatgcg aaaggtagtg
    69961 tgtttaattc tccaaatatt atcggtgtag gtgaatcagg acctgaagca gttttacctt
    70021 tgaaaagatc tgtgctgcaa gaaattggtg atcgtatctt gagtagcacc tcagtttcat
    70081 ctaggacaca agcggttcaa cctgtgaaca attacgaatt caatttcaca attgatggta
    70141 acgcagatga agtcactatg aagcaaacaa ctcaacaaat cattgatagc attacaaaag
    70201 ttcaaaatga taatgcttcg gcatggcgtt aaacaggaga gtatttctcc tgttttttta
    70261 gtattaaaaa ggatgtgaaa aaatgactga ttgtatacat tctataatcg atggatttcc
    70321 tgattatttg cataaattgg ctttagctga aagaccaacc ataccttctc caaaaagaca
    70381 gagagttgaa acttctgttt taggaaggtt aggtggctta gtgcaagatt actcgtttga
    70441 agacatgtcg tttacattgc actataacta tttagaggat gtggaagacc atcaagcgtt
    70501 caagcaatcg ttttatatca tgcgtcattg gttaaactat gcaaagaaat tagaattctc
    70561 tgacgatccc aacgtctatt acgttatcca gactatcgat attggggatg cagaaaacga
    70621 tattgttgaa tggggagagt tcgatgtaaa tattactgcg aaaccattcg caagagttca
    70681 agaagatgta cctataaccg tagataaacc acagtcattt aacttgctga ataatagttt
    70741 agaagaaagt tttccaaaga ttatcatcac tccttcagct acttcatgcc agttcatctt
    70801 aaatgattat gtgtttagtt tcgaaggctt agtagcagga actgatgtag tcattgatag
    70861 tgatttgatg ctttgctacg aagagcaatc ggacggagat attttagatc ggtccaacaa
    70921 aatgaagacc atgcaatatc cgacattgca agtggatatc aatcatttta attgcactgg
    70981 tttgagcaaa atacaaattt atcgtaacgg gttaaggtag gtgaaataga tgatcgataa
    71041 tttaataact atttacgata aaaatgacgc gaataattta gctgaacatt tatatgatac
    71101 gcaaggttta ggcgctttgt cagactggtt aacagctact gttagcaata aactaaacgg
    71161 agccgagata tttcagggta cttattcaat aaacggaact aatgcagatt tgattgtaga
    71221 aggacgtatt attcagtgtt atgtagatga aaatcgagca aaacagcgtc tacggattta
    71281 ttatgcaaaa acttctgtaa taggcaatac gatagaagta aaagctgaac ctattttcaa
    71341 tgatataaga aaatcggtgt tgaataaata tgacagcgga acagaaaaaa tcactgctac
    71401 tcaggcatgg caaaacgcaa aagttttagc gaaaccagct atcccttcgc agttttcttt
    71461 ctcgtcatta gtagatacgc ttgctaatgt gaagatagaa aaggcgaatt ttttagaatt
    71521 ctttggtgga aaagagggat ctattctaga tcgatttcat ggtgagtttc taaaagataa
    71581 taacacatta cgtcatgaaa aaagtctagg tacggatcat aaaatcaaag cgatttatac
    71641 taaaaactta actggtcttg acttagagat tgatgctcaa agtgttttag ttggagttta
    71701 tccatttatt agcagctctt cagaaggaga agacgagatc actctaccag aagaagttat
    71761 tttcacggat tacgtggatg attatcctgc tggatatgtt tcttttgttg attttaaaga
    71821 caaagcgact gatgtagcca cattaaggga agctgctaaa gactggttga aaacaaacat
    71881 agataaacaa aaaccacaag tgagtggttc gattgaatta gtaccattga ggcatcaaag
    71941 aggctatgaa aaatttgttg atctagaaaa agtttcgatg ggtgacggag tagatgtgta
    72001 tcatccacag ttaaaagtga atatgtcagc gagaattgtg gaatatacgt ttaatgttct
    72061 aaccaattca tacgataaat tagttgtagg aaacgtcaaa acaaacttct tagaaaacac
    72121 agagaataat gtaagcaatt tgattaatga tgccattgat caattgaaaa atggtggcga
    72181 aatcagtgat ttaatcaatg atattgtaga tcatcaaact gatatgatta ctggtcaaaa
    72241 tggtggttat gttttattag atcctaaaga agcgcctagt cgtattttga ttatggacac
    72301 accagataag aataccgcaa ggaatgtttt acaaatcaac aatgctggta ttggtttttc
    72361 taaaactggc attaatggaa catatgaaac cgcatggaca ttagatggcg gattcaatgc
    72421 ctcgtttatt acggctggtg aaatagtagg aattactatt agaggtacta cattaattag
    72481 tgatggcact gattatagaa caagtattgc taatggcaaa atgacttggt actcaaaaaa
    72541 agttaacaaa gatattatgg agctagaagc acgtgattat gtaagtgctg atgccggtat
    72601 tgtatcatac accatgaaaa ctggtggtgg tttcatgatt agaaatccac agggtaactt
    72661 ggtttttagt acgtgggata atggtaataa cagaccgttt ctatcttttg gtgcgcccaa
    72721 tttcaggtat agcaatgcta gttatgtaac ttctggcgac ggtagttctt taagcattaa
    72781 tggtagtgcg ggtaactcat gggaatttaa ggtagctggt aggactatga aatttactag
    72841 tgatggtatg ctaacgttgc caggttgttt ttttggttca tgggaagatg ggaaacttgc
    72901 taggtttgaa caatcaacgg tacaagtata taaagatttt actgttagag gtactaaaaa
    72961 ctcaaccgta ccaacagaac attatggaca acgactatta aacgcttatg aaactccaga
    73021 atattatttc gctgattatg gggaagccgt tacaggtgat gatggtaaag ttcgtgttga
    73081 tattgacccc atgtttgctg agacagtaaa tctaagtcga tatatgacac atgtgacacc
    73141 tacagaacta gttttgtgtg ctgttactca tgaagatatt gaccatttca tcattgaaac
    73201 tagtaagcca aacgtattag ttagatggaa tttagtggca caccgtctag ggtatgaaga
    73261 tattagatta aaagaggata cagcatatga tagcacagtg cttgaccaaa aacgttttta
    73321 aaacgaagac aaggaggtat ataaatggct agcagtttat ataatttggc tctagatttc
    73381 agcaaagaat taaactacac caaagctatt atggctcgtc aaggtgataa agggattaca
    73441 gtgactgtta aaccattttt aaatggcttg cagatggata cgagtggcgg aacatttact
    73501 ttaaaaggaa caacgccatc taaccgttac gtagatagtg tggcaactag tgtaactagt
    73561 gaagaagtca cgttttctct tgatggcaca tttatgagtg aagcaggata ttataaacac
    73621 tgctacgtag aatatagaaa agacaatcaa attttaacaa cgcaagatat catttttttc
    73681 tcactaggag tgtctgacat ttcgcaaggc caagccgatg aatacgtttc gcaattggaa
    73741 gagttgattc aaaagtataa cgaaactttt gatgctttta tggctgaaat cgaaggtaga
    73801 gtggacagct taaatcaaca gattactgat ttaactggtc aagctaaaac actacaagac
    73861 aagttagacg ctctgaaaga agaaatttct aaattaggta acttgcaggt gatgtacagt
    73921 aacagcatcg acttcggggg atatgattat agcgggaatc ctaatttgtt aagtaagcta
    73981 tcatacgact taattgaaaa tcaaaatact tcagctggaa cactttctaa aggtgaaaac
    74041 tcgtttaaat ataataagat atcagctgaa acggaaggtg gagtagagtt atattataaa
    74101 cgaagaggta tagctaactg gttaccctct aataaaacgc ttgtaatgac cgttaaactt
    74161 agagctggag ttgactatag tccagctgac ggaaaaaaaa tactgattag atataggttt
    74221 gttgacaatg gaactggcaa gattgtttta gacttaccta ttaacagtaa ttcgataact
    74281 caggaatgga aagagtttag tattactgga actactccaa catttggccc gcaagcatac
    74341 catccttgga tacaatttag ggctcaagat gggatacttg gggaaataga aatgagctat
    74401 gacatcaaaa tcgaagaagg tccaacagcc acaccgttcc aacctaactt attggcagaa
    74461 ccttataaca tgtgccgcga gtatcctaac gagaacattg tcaatcctac agttaagttc
    74521 ccaattaaaa catcaaataa tcctatttct ggttttgata tttcagaaga attaataata
    74581 ggtgaaactt atacggtatc tttaaaaggg actaagccag ctactaaaga attccactta
    74641 tactacggag aagcaaatta ccagcagttg cagcatcagg caactttact tcctgtggaa
    74701 ggattaacag atgtgtggag tgccactttt acagccagaa atactagtga aacgacaaat
    74761 ttacttagag tagcgttatg gcaaaaacct aattctgcaa catatgatac tgttcaaatt
    74821 gactggctca agattgaaaa aggcaacacc cgaacgccca atattagtga gtataaatat
    74881 cgtggtactg gtatgcgtga ttcaaacaat ccaaaagatt atgtttggga tctagcacca
    74941 gaatatgtcg aagataattt ggccacagat attaaaattt ctgaaattac tggtaaagca
    75001 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
    75061 gaagtagaca ccacagctaa ggatgctcga acaaaagcga atgctaatgc gactgctata
    75121 gatgaattag acaataagat cgatgaacgc attaatgatg cagctactac cgcattttat
    75181 ggcgcacaag gtgaagtctc taacaaaacg aaagtagcgg agtatggagt aggaacaggg
    75241 tatactacta cagctgtagg agaaacacat ttcgaacgtc aatctaacgg atacgttaaa
    75301 tgcttaaaag ctggtaagta ttcaattagt gcgcaaatac gtgtgcaatt gggcgggaaa
    75361 tatgcttcat ggttatatac tgatttatat aaagatggaa cttctgttga taatttagta
    75421 tcttatgggg tcgacgcctt gcaaaataga tttgcagcta gcggaaacat tgtaactgat
    75481 ttagaagtaa acgatgtact aaatatgcgt actgaaatcg gcgttgcaga tggaatttta
    75541 caatttacat cttgccgtac tttagtcttg actagactat ctgattaata caatatttat
    75601 aattttgcta tcaacacgct caaaggaggg tgttttttat tttgcaatga aaggaggcta
    75661 gttggttgaa agacgaagca atacaagacg tggtagaacg cttagtgcgt attgaaacga
    75721 aactggataa ttacgaatta ttacgtgaaa aagcggaaag tgcaaaagat agagcggatc
    75781 aggtatattc tattgcgctt aataatgcag aagacatcaa agaaatgaaa gccaataata
    75841 aatggtcgtg gggttacatg attggtttag gcattacgat cattggctat ttcttgacta
    75901 aattgtaaag gaggtgagaa gaaatgattt taccagataa gtattatcaa gtcattaaat
    75961 ggacagtttt aacagtattg ccagctgcat ctgctttagt agccatgtta gggaaagcgt
    76021 atggatggaa tggaacagat atgatagtgc tgactatcaa tgcagtagca acatttttag
    76081 gcgttatcac tggtgtgtcg gcttataatt tgaaaaaata ggaggaaaca aatgaaaaag
    76141 aaaattacta ttactgcgat gagcctgtta acggctcttt ttttattgcc aattaacgga
    76201 tttgcctata ctatcaacaa tgaatttaat ttgggcgcaa atgaaggtag ctcacaagta
    76261 gcaaataatc agtacatttt actgcatgaa acggctaatg aaacagcaac aggacgcaat
    76321 gaagcgcagt atatgcaacg ttcatggact agtgcttaca ctgcttacat tgtgggtgat
    76381 ggcggaattg tttaccaagt tggacaacct ggttatgtac agtacggtgc tggttcgtat
    76441 gctaatgcca acagtcctgt gcagattgag ttacaacaca cacatgataa agcaacgttt
    76501 gagaaaaact acaaggcata cgttgaattg gctagagatt cagcaatgaa atatggtatt
    76561 ccattaacgt tggacactcc ttataaccaa ccgggaatca aatcgcattt atgggtaaca
    76621 caaaacatct ggggcgatca tacagatcct tatggttatc tttctgaaat gggcgtaagt
    76681 aaagaaaaat tagcatatga tttagctcat ggatttaccg atgaaaatcc gacaacttca
    76741 gatgataaac cagtcattga tccaactaga gcaggtgcag caaatcctac gctgacagat
    76801 ggaacaaatt acgcccacat tgatcagttt ggggaaatcg aaaacgcaaa cttgcatgtg
    76861 gctggatggc acattgctaa ctataaatac gagtatatct tcattatgga ctacaatact
    76921 ggaaaagaac tagctagagt aagagctgat gggatttata gaccagacgt aaatcaagct
    76981 tataatactt taggaaatgt tggctatcat gtatctttca atatgcgtaa ttttcctaat
    77041 aagaaagtct atgtaatgat gcgggcaacg aatgatccag aagggaacac taaaggcgga
    77101 gcacaagatt ttcatgataa acgctggtac ttgaatattc ctaaacgata aaaatagccc
    77161 ctc
//
LOCUS       DACECY010000006        72432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.006, whole genome shotgun sequence.
ACCESSION   DACECY010000006 DACECY010000000
VERSION     DACECY010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.006"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(159..443)
                     /locus_tag="IWO38_000409"
     CDS             complement(159..443)
                     /locus_tag="IWO38_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312526.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(461..1351)
                     /locus_tag="IWO38_000410"
     CDS             complement(461..1351)
                     /locus_tag="IWO38_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ0312527.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1371..1589)
                     /locus_tag="IWO38_000411"
     CDS             complement(1371..1589)
                     /locus_tag="IWO38_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312528.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1559..3259)
                     /locus_tag="IWO38_000412"
     CDS             complement(1559..3259)
                     /locus_tag="IWO38_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ0312529.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3526..4299
                     /locus_tag="IWO38_000413"
     CDS             3526..4299
                     /locus_tag="IWO38_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312530.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4380..4949)
                     /gene="thiT"
                     /locus_tag="IWO38_000414"
     CDS             complement(4380..4949)
                     /gene="thiT"
                     /locus_tag="IWO38_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ0312531.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5015..5117)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5123..6094)
                     /locus_tag="IWO38_000415"
     CDS             complement(5123..6094)
                     /locus_tag="IWO38_000415"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ0312532.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6188..7561)
                     /gene="glmU"
                     /locus_tag="IWO38_000416"
     CDS             complement(6188..7561)
                     /gene="glmU"
                     /locus_tag="IWO38_000416"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ0312533.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8064..8882)
                     /gene="purR"
                     /locus_tag="IWO38_000417"
     CDS             complement(8064..8882)
                     /gene="purR"
                     /locus_tag="IWO38_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ0312534.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9049..9873)
                     /locus_tag="IWO38_000418"
     CDS             complement(9049..9873)
                     /locus_tag="IWO38_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0312535.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9846..10526)
                     /locus_tag="IWO38_000419"
     CDS             complement(9846..10526)
                     /locus_tag="IWO38_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0312536.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10549..11487)
                     /locus_tag="IWO38_000420"
     CDS             complement(10549..11487)
                     /locus_tag="IWO38_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ0312537.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11732..12583)
                     /locus_tag="IWO38_000421"
     CDS             complement(11732..12583)
                     /locus_tag="IWO38_000421"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ0312538.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(12737..15310)
                     /gene="mprF"
                     /locus_tag="IWO38_000422"
     CDS             complement(12737..15310)
                     /gene="mprF"
                     /locus_tag="IWO38_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ0312539.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15451..16257
                     /gene="proC"
                     /locus_tag="IWO38_000423"
     CDS             15451..16257
                     /gene="proC"
                     /locus_tag="IWO38_000423"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ0312540.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16336..17892)
                     /gene="rny"
                     /locus_tag="IWO38_000424"
     CDS             complement(16336..17892)
                     /gene="rny"
                     /locus_tag="IWO38_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ0312541.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18303..19352)
                     /gene="recA"
                     /locus_tag="IWO38_000425"
     CDS             complement(18303..19352)
                     /gene="recA"
                     /locus_tag="IWO38_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ0312542.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19461..20702)
                     /locus_tag="IWO38_000426"
     CDS             complement(19461..20702)
                     /locus_tag="IWO38_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ0312543.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20942..21520)
                     /gene="pgsA"
                     /locus_tag="IWO38_000427"
     CDS             complement(20942..21520)
                     /gene="pgsA"
                     /locus_tag="IWO38_000427"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ0312544.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21729..22613)
                     /locus_tag="IWO38_000428"
     CDS             complement(21729..22613)
                     /locus_tag="IWO38_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0312545.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(23391..24677)
                     /locus_tag="IWO38_000429"
     CDS             complement(23391..24677)
                     /locus_tag="IWO38_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ0312546.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(24670..25938)
                     /locus_tag="IWO38_000430"
     CDS             complement(24670..25938)
                     /locus_tag="IWO38_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ0312547.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            26190..26651
                     /gene="mscL"
                     /locus_tag="IWO38_000431"
     CDS             26190..26651
                     /gene="mscL"
                     /locus_tag="IWO38_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ0312548.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(26710..27156)
                     /locus_tag="IWO38_000432"
     CDS             complement(26710..27156)
                     /locus_tag="IWO38_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0312549.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(27249..27494)
                     /locus_tag="IWO38_000433"
     CDS             complement(27249..27494)
                     /locus_tag="IWO38_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312550.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(27609..28187)
                     /locus_tag="IWO38_000434"
     CDS             complement(27609..28187)
                     /locus_tag="IWO38_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0312551.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(28264..28794)
                     /locus_tag="IWO38_000435"
     CDS             complement(28264..28794)
                     /locus_tag="IWO38_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ0312552.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(28798..29658)
                     /gene="rlmB"
                     /locus_tag="IWO38_000436"
     CDS             complement(28798..29658)
                     /gene="rlmB"
                     /locus_tag="IWO38_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ0312553.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(29651..30046)
                     /locus_tag="IWO38_000437"
     CDS             complement(29651..30046)
                     /locus_tag="IWO38_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ0312554.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(30043..31455)
                     /locus_tag="IWO38_000438"
     CDS             complement(30043..31455)
                     /locus_tag="IWO38_000438"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ0312555.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(31452..31988)
                     /gene="cysE"
                     /locus_tag="IWO38_000439"
     CDS             complement(31452..31988)
                     /gene="cysE"
                     /locus_tag="IWO38_000439"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ0312556.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(32310..33770)
                     /locus_tag="IWO38_000440"
     CDS             complement(32310..33770)
                     /locus_tag="IWO38_000440"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ0312557.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(33940..35097)
                     /locus_tag="IWO38_000441"
     CDS             complement(33940..35097)
                     /locus_tag="IWO38_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ0312558.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(35131..36501)
                     /gene="radA"
                     /locus_tag="IWO38_000442"
     CDS             complement(35131..36501)
                     /gene="radA"
                     /locus_tag="IWO38_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ0312559.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(36564..37091)
                     /locus_tag="IWO38_000443"
     CDS             complement(36564..37091)
                     /locus_tag="IWO38_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ0312560.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            37321..38127
                     /gene="proB"
                     /locus_tag="IWO38_000444"
     CDS             37321..38127
                     /gene="proB"
                     /locus_tag="IWO38_000444"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ0312561.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            38124..39371
                     /locus_tag="IWO38_000445"
     CDS             38124..39371
                     /locus_tag="IWO38_000445"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ0312562.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            39447..39791
                     /locus_tag="IWO38_000446"
     CDS             39447..39791
                     /locus_tag="IWO38_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312563.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(39908..40234)
                     /locus_tag="IWO38_000447"
     CDS             complement(39908..40234)
                     /locus_tag="IWO38_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312564.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(40607..41808)
                     /locus_tag="IWO38_000448"
                     /pseudo
     CDS             complement(40607..41808)
                     /locus_tag="IWO38_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            41925..42440
                     /locus_tag="IWO38_000449"
     CDS             41925..42440
                     /locus_tag="IWO38_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0312565.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(42639..43085)
                     /locus_tag="IWO38_000450"
     CDS             complement(42639..43085)
                     /locus_tag="IWO38_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ0312566.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(43132..44610)
                     /gene="msr"
                     /locus_tag="IWO38_000451"
     CDS             complement(43132..44610)
                     /gene="msr"
                     /locus_tag="IWO38_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0312567.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(44964..45593)
                     /locus_tag="IWO38_000452"
     CDS             complement(44964..45593)
                     /locus_tag="IWO38_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0312568.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(45577..46041)
                     /locus_tag="IWO38_000453"
     CDS             complement(45577..46041)
                     /locus_tag="IWO38_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0312569.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(46528..47796)
                     /locus_tag="IWO38_000454"
     CDS             complement(46528..47796)
                     /locus_tag="IWO38_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ0312570.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(48027..48764)
                     /locus_tag="IWO38_000455"
     CDS             complement(48027..48764)
                     /locus_tag="IWO38_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0312571.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(48831..49400)
                     /locus_tag="IWO38_000456"
     CDS             complement(48831..49400)
                     /locus_tag="IWO38_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ0312572.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            49528..50400
                     /locus_tag="IWO38_000457"
     CDS             49528..50400
                     /locus_tag="IWO38_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0312573.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            50742..51614
                     /locus_tag="IWO38_000458"
     CDS             50742..51614
                     /locus_tag="IWO38_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0312574.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            51611..53569
                     /locus_tag="IWO38_000459"
     CDS             51611..53569
                     /locus_tag="IWO38_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0312575.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(53820..54671)
                     /locus_tag="IWO38_000460"
     CDS             complement(53820..54671)
                     /locus_tag="IWO38_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0312576.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            54794..55261
                     /locus_tag="IWO38_000461"
     CDS             54794..55261
                     /locus_tag="IWO38_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ0312577.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(55524..56006)
                     /locus_tag="IWO38_000462"
     CDS             complement(55524..56006)
                     /locus_tag="IWO38_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0312578.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(56106..56969)
                     /locus_tag="IWO38_000463"
     CDS             complement(56106..56969)
                     /locus_tag="IWO38_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0312579.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            57567..58415
                     /locus_tag="IWO38_000464"
     CDS             57567..58415
                     /locus_tag="IWO38_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312580.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(58586..58927)
                     /locus_tag="IWO38_000465"
     CDS             complement(58586..58927)
                     /locus_tag="IWO38_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ0312581.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            59013..59720
                     /locus_tag="IWO38_000466"
     CDS             59013..59720
                     /locus_tag="IWO38_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ0312582.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            59707..60030
                     /locus_tag="IWO38_000467"
     CDS             59707..60030
                     /locus_tag="IWO38_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0312583.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(60119..61156)
                     /locus_tag="IWO38_000468"
     CDS             complement(60119..61156)
                     /locus_tag="IWO38_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0312584.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(61603..62586)
                     /locus_tag="IWO38_000469"
     CDS             complement(61603..62586)
                     /locus_tag="IWO38_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ0312585.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(63002..63772)
                     /locus_tag="IWO38_000470"
     CDS             complement(63002..63772)
                     /locus_tag="IWO38_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0312586.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(63765..64700)
                     /locus_tag="IWO38_000471"
     CDS             complement(63765..64700)
                     /locus_tag="IWO38_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0312587.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(65294..66943)
                     /locus_tag="IWO38_000472"
     CDS             complement(65294..66943)
                     /locus_tag="IWO38_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ0312588.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(67138..68034)
                     /locus_tag="IWO38_000473"
     CDS             complement(67138..68034)
                     /locus_tag="IWO38_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0312589.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(68027..68707)
                     /locus_tag="IWO38_000474"
     CDS             complement(68027..68707)
                     /locus_tag="IWO38_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0312590.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(68708..69490)
                     /locus_tag="IWO38_000475"
     CDS             complement(68708..69490)
                     /locus_tag="IWO38_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0312591.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(69483..70400)
                     /locus_tag="IWO38_000476"
     CDS             complement(69483..70400)
                     /locus_tag="IWO38_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0312592.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(70403..70687)
                     /locus_tag="IWO38_000477"
     CDS             complement(70403..70687)
                     /locus_tag="IWO38_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0312593.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            complement(71388..72008)
                     /locus_tag="IWO38_000478"
     CDS             complement(71388..72008)
                     /locus_tag="IWO38_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312594.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            complement(72089..>72432)
                     /locus_tag="IWO38_000479"
     CDS             complement(72089..>72432)
                     /locus_tag="IWO38_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312595.1"
                     /translation="NIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQDK
                     KNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQKK
                     RDNNDQYLPKK"
BASE COUNT        20828 a        15441 c        12751 g        23412 t
ORIGIN      
        1 ataccgagaa aatggttttt gaacatcact tattcaaaat aaaaaaagat tgtgacacga
       61 attttgtcac aacctttaaa tgtattccta gagcaaagaa gctaagcgaa gaaatccaat
      121 tctgattatt tattgtattt ctttgttcag catccttgct aaaagcccat attggacaaa
      181 gtattcacta cacccatgat ctcatctttt cgagaacgtg ctttttctgt cggatcttct
      241 acctcttctg tgtcaattcg aacaataaat ttccgattca tcgtcacgta agcaccgtcg
      301 ttgatcaaaa taaacagttc gttctcatta tagtctgcac aggcatcttt ccatacgtca
      361 aagccgtttt tttcagaagg gatatcccgt ttaaccacag tttttccact taattcgtgg
      421 aacgttacgt cataatattt cattatctct acctctttcg tcattcaata ttttgtactt
      481 gttctctgat tttttctaag atcgtcttag actggacgac ttgattttta atttcgattg
      541 catttgattt ggaaccgatc gtgttgattt cccgattcat ttcttggatc aggaaatcta
      601 attcccgacc tacaggacat tgtgcttcga taagttcatc tagtttttgt acatggatcg
      661 ccagacgatc cagttcctcg tgaatatctc cgcgttccaa caagatggct agctccgtca
      721 acaaccgttg ctcatcgact ttcgctccaa gaaattcctc cagctttttt tgatacctta
      781 tttgaaattc cttttcatag atttctacaa aactatcgag tttttttagt acttctttga
      841 attcttcagt attctttttc aacactaaag ctaaagcttg accttctttt tctcgactcg
      901 cctcaataga atcaagtgcg cttttcaatg tatctagagc taactgtgcc aattcttcca
      961 tgctctcttc cggtttttct tctaccgtta cgaagttttc gtccagcgcg aagcgttccg
     1021 ccatccgact aattgacact tgattttgac taccgtatcg ttgttgaatt ccgtctgtca
     1081 attgagtgat aagtgaatca atcaattccc agttgatgac gatctgtttt tcgtttttgc
     1141 caatataagc tagattcaca aatacttcga ttcgaccgcg gcttaactgc tgtttaataa
     1201 gctgacgaaa atcgttttca acaaagttca ggattttagg acttcgtatt tgaacatcta
     1261 gaaaacgctg gttgacactt tttatttcaa tatctaactg atacgctttt gtttcgcgga
     1321 tggctttgcc aaaaccagtc atacttttca ttcgacaggg tccttcctca ttacatattt
     1381 tctattgctt tttgcagttg ttcaaactct tcgttcgtca atgtcaggcc tttccccatt
     1441 ttttcatggt ctggcgccca atctcgaaga tcgaattttg gcgggcgacc attccagctg
     1501 actaggttca gttctttacg ccaccccttg ttgttttctg acaatatagc gatttcttct
     1561 acgatttcat atgaaaattc ttgtgccatg gttgttcctc cttgagataa tataaatata
     1621 ggatcagtaa tgtgtcaact ggttgcttaa gcaattcatg ctgcaaacga tccttgcgtt
     1681 ttccttgtag cagataatca aaataaacag ttttcgtgcg ctcattttgt attttttgct
     1741 ggtattttcg ataccttcgc cattcttcag tacttaattg accaatttgt gccaataaat
     1801 catccggcat actttttata tcttgttggc agatcatttg aatcatcatg tctacttgat
     1861 agatttcatt gggcagtaaa aaatgttttt ttgctaaaag ttctagcatc gccaatccgc
     1921 atgtctctgg tacgaccctg ttcttatata aattacgcag tttttgccaa aattttatgc
     1981 ttttgaaaaa agataattct aactgaattt cttttgttaa gtaagacaaa aatcctcgcc
     2041 agtatgttct tgcaggttta acagaatgaa atgtatacca gttcccatag taatagccta
     2101 atcgtttttt catttccaga tagcctaatt gtaaatttaa ttggttcctt tggctatcaa
     2161 aaagcagaaa gctgcctagt gtccacaatg tttgacattt ccaatggata aaaatgtctg
     2221 gcatacgaat ttttttcgct gggcctggcc cttgcacatc tacgacaaat gcctctgttg
     2281 ctcctttgtt aatcgcaata tcaataggta ttttgttgcg atacccaccg tctgcatatt
     2341 tattttttcc gatctttcga taagccatag cgggataaaa tgaagcagag gcgagtatcc
     2401 aatctggtat ttcttctgtt tttgtctgtt ggatatcagt cactacttcc gctagttctg
     2461 gcagtttagt agacaccgta tataaacgta ttttgggatt atgtttgagt aaggacaaat
     2521 ccaaattgtc tttgaccatc ttttccaatg gagcagaaga aaccccgcct tcaattagtg
     2581 ctgttttcgt catttggcgt ctttctcgat agaagcgttc acgaaggtct tcaactgcag
     2641 ccatttctgg taacgcaagc acttgtcttg tcgacagctt tttccataaa ttcacagctt
     2701 ttttcatatc tccttgtaaa atcaatgcgc cgttcaacgc acctaccgag gtacctgtga
     2761 taatctcaaa gttgatatca tgttccttca gcgcctgcca cacaccaatc tgataagcac
     2821 cgtgcgctcc tcctccacct aaaacaagtg ctgtatgata gaccagcttt ttatttaatt
     2881 gtgtggttcc ttgatagccg tttttatgaa aaatacgcaa ccattcttct gatagaggag
     2941 attgaaataa aaaacaacag ttttctttaa taaaaaaacg agcgcaagct tccatatacc
     3001 gaaaaaattt tttccattca aaagtttcac cttcaatcaa cagattatta aaaataacat
     3061 ctcctctact tttagcaata gtaaaataga cggtatattt tttcgatact gctttaaaag
     3121 cacattgctt ttttgcagaa cgccaaaaag cgtctctact ttcttgatga ctttgaaaaa
     3181 atggaagggg ttgttttgct tgctggatag gcgaatacag cgacattttc tgtgaaaagt
     3241 aaactttttt tatctccatc gcgctctcct ttttcttcaa gtataccagt acgtcataaa
     3301 aaggtcatga gtctcgtatt cgactttgta gattccataa gaaaacaatt cataaaacct
     3361 cctttaaata aaaaaataca aaaatcacaa aaattaattg aaaatgagag tgttttcaat
     3421 gaaaacgtga ttttgtaatg gaattttaaa ataaaagaaa caagcttttt taatgcaaat
     3481 gttgtaaaat aaaagagatt aagaggaaat aagaggagga acatgatgac tgagtcagtt
     3541 tatgtacata ttgataccac aagtaatgct gttttaacaa aagggctgac tgctactgac
     3601 tttgcaaaca gtatcgtgca ttttcctcaa aacttgctct tgcttgatcc atctgcttct
     3661 gctggagaat atgaatccca tacagggctg aaagtaatcc gtggtacaga aaatattcag
     3721 cgtttctttt cctcttctcg taacaggcgt ggattcgatg aattgaagtg gatcgacttt
     3781 actgatttaa cgatgctgaa agaattgaca cctttagaaa tttcagaatt attatatttt
     3841 ggtcatatga aaacacatct tcattcgccg tttttttata aactacagaa taattttgta
     3901 tacttcgatc ttaatgatca gctgaatcgg atttattatc gctatttaga tgaattttat
     3961 aggatttttg ctagtaaatt aagttcgatc ttgtcagata agatcaatgt aaaaaaatct
     4021 tttttcaaaa aagaaacacc tgttgaaaaa atcagtgtgg aattattgaa agaaatgcgt
     4081 aatttgatgc aggaagggat cattttttct tttgatcaag ttggtttgat cgatggtaaa
     4141 tatcgtatcc caatctatgt gattgaagat tatatttgga aattaaaagg cacccggtat
     4201 cgagaagagg aaatcatcgc tactttaatc tactctgtca ccgatcgaca ctggcaattg
     4261 gtagaagatg ccgatctttc ttatctgcca ttgaagtaga tctatttctt atcaaaaaat
     4321 aaaaaggaaa caagaaacta ggacatgatt ctgtctcaag tttcttgctt cttttttatt
     4381 tacagctcct gtcgtttatc tggcacaaaa atcgaaggag tcgtttgttg gatccctaat
     4441 aaaacgatga tcgttacaat agctgtactc aacccgctgg caccattcat tacgaaagaa
     4501 aagagccaag gattcatccc ccatagtgca tacgctcccc agaaaaccac tcctgcaata
     4561 aaatgccaaa aatatctgga aagtgcgcca atcaatgcac caaacacgat gctttttcta
     4621 atttgtttcg tattatcgtc tacatagcct ttttggacat ttgaagccat gagccctgct
     4681 gcgcctgcaa aagtataggc aatcgggtac tcaatcaaca cttgaacgac acttaaataa
     4741 taaacttgtc ccaacggaaa atgtaggatg ccccaaatga aacctgccag catccctgcc
     4801 tttgtccctc ttcttaacgc aaaaagagtc aatggaatct gccctaatga aatagaaaaa
     4861 ctgctgccga tctggatcgg aatcaacgac aacaccatcg ctaacgctgc aacaatcgtt
     4921 ccttctaccc aaactctatt ttttcccata aaaaaacttc ctcctttgaa taaaaaagtc
     4981 acaaaggagg aagcagcttt cgaaaatagt caaacagctg tttattcaca attcctacgc
     5041 tcgcataacc gatacaggta cgaagggttt agaaatagat tcaatctcag cccaattgga
     5101 ctcccctttg tgagtttatt tattatttat tttttgtttc aaacaatgga ctaactggtt
     5161 tgttttcatg gatacgtttg attgcatctc ccattagtcc gccaacactt acttcatcga
     5221 ttttgtcgat tttacgatct tctggaagat agatcgagtc tgtaacaact agacgctcga
     5281 tagctgaatc ttctattctt tgtaaagcag ggccggataa tactggatgt gtacatgaag
     5341 catacacact agttgcacca gcttctttca aagcattggc agccaatgtg atcgtacctg
     5401 ctgtatcgat catatcatcg atcagtacac aagttttgcc tttgacttgt ccaatgatat
     5461 tcattacttc tgcaacattt gcttttggac ggcgtttgtc tatgatcgca atcggagatt
     5521 tcaaaaattc cgctaattta cgtgcccgtg tgacaccacc atggtctggt gaaacaacga
     5581 caacatcatc gcctttgatt cctttttcta agaaataatt ggcaataagt ggcgcaccca
     5641 tcaaatgatc gactggaata tcaaagaaac cttggatttg aactgcatga aggtccaaag
     5701 tcagcattct tgttgcacca gctttttgaa tcatgtttgc tactaatttc gctgtgattg
     5761 gttcccgtgc acgtgcttta cggtcttgac gcgcatagcc ataataaggc atgactacgt
     5821 tgattgtttt tgcacttgct cgtttcagtg cgtcaatcat gatcagcaac tccatcaagt
     5881 tatcattgac tgggctgctc gttgattgaa taacatagac atgcgatcca cgaatacttt
     5941 cttcaatgtt tacttgaatt tctccatcac taaattgagt aacagatgat tttcctaact
     6001 cgacgccgac tgctgctgca attttctctg ctaatgggcg attggaattt aacgcaaaaa
     6061 tttttaatct tgggtcaaag taatgttttg acatggggac ctccactttc tttttcacaa
     6121 ctctagaatt ctacctaata aatagtagtc atatttttta aataaatcaa gtgattttgc
     6181 aaaagtctta gtttagataa gggagttttt ttgcatatcc ttctttattg acttgtcttg
     6241 ctctcgcaat tgccagcgca tattctggaa tattgtctgt aattgttgac ccggcagcaa
     6301 tcgaactgtt tttagctacc tcgacaggtc cgatgatatt cgtgctagaa ccaataaagc
     6361 tgtggtcgcc gacagtcgta tgatgtttgt tctttccatc atagttgacg aagacaacac
     6421 cacatccgac attgatttct tctcccagcg tagcatcacc aacataagtc aagtgaccga
     6481 ctttagttcc tttgccgatt tgagcatttt tcacttccac aaagtttccg atatggacgt
     6541 tttcgccaat ttcagctttt ggtcgtaaat gtgcataagg tcccacatct gcatgtttct
     6601 tgacatggct gctttcaatg actgagtttt caatgacgac gtgatcctca atcctactgt
     6661 ctacgatttt tgaatgtgac ccgatgacac aatcagaacc aatgactgta tttccttgga
     6721 tttgtacacc tgcttcgatc aatgtgtctg gaccgatttc tacgcctgca tcgatgtatg
     6781 tcgttgctga atcgacaaag cttacgccat tgaccatatg catctggtta attcgctttc
     6841 tcatgatctc atttgcttta gctaacgcga tccgatcatt gactcccatt gattcttcaa
     6901 aatcatccgt ttgataagca gcaactgtat ggccttcttc ttttaagatt tcgatgatat
     6961 ctgttagata gtattcacct tgagcattgt ttgttccaac tttgcttaac gcatcgaaca
     7021 aagcttcgtt gtcaaaacag taagttcctg tattgatttc ttgtacaaga gcttcttctg
     7081 gtgttgcatc tttttgttcc acgatttttt ctacgatccc aatatggtca cggatgattc
     7141 gtccatatcc tgttgggttt tctgcttgtg ccgtcaagat cgttgcactt gcattttttc
     7201 cttggtgata ttcaaaaaga ttgttcagcg tttcagtagt caataatggt gtatcgccac
     7261 tgataactaa cgttgttcct ttttttcctt ccaagaaaga agcagcttgc aaaacagcat
     7321 gtcctgttcc caattgttct gcttgtaacg catatttact gcgttctcct agttgtgctt
     7381 taacttgttc tgctccgtgt ccaacgatcg taatcacttc atctggtttc gtttcactga
     7441 cccgattaat gatatgttct accataggtt taccagaaac gggatgtaaa actttatata
     7501 attttgactt catgcgggta cctttccctg ccgccaaaat aattgcataa cgtgcgtcca
     7561 actgcgtcac tcctaaatat agtttgctga tttcagttta tcccagtata cccatttttt
     7621 caacaaaaca agtgattttc ttttatccca gcgttttctg gataaaaaaa tagatagacc
     7681 ataacgggcc tatctatttg gtaaccggtg ctctcctgcc aactcctcgg tcacaagtca
     7741 cactactcat aaaacatgaa aagctgtttt ttgaaatcgt ttcttgtgac ttagagttaa
     7801 acggtaggct tacaacctct tcattaaacg tttttcaatc agctgacctt cggtattagc
     7861 tcaataaacg gcaaaatatg aggagcaatt tttccgttta ttgagctaat actcaggaca
     7921 ggacgctgat ttcacaacct ctagataaac ggtgttcaaa agccagtttc tcggtattaa
     7981 gccaaattta tgaaaaaatg tggaaagcca ttttcacaaa atttttctta ctactcgaaa
     8041 ctaagcagct ttttcacaac ctcttattct gaaaaataat ttcctggcac aaccgtaata
     8101 tttttcgttt gagtatctac atctttgaca taaagcagtg acgtataatc atcgatggcg
     8161 cgatgaccgt tgaattttga ttcggcaaat acagtgatgc caacaagttc tgattcgaat
     8221 tcttcaatca agctcttcat tccgttgact gttccgccgc ctttcatgaa atcatccact
     8281 acaaggacac gtgaaccacg tttcaaactt ctcttagaca gttccatctt ttcaattctt
     8341 tcagaagatc cagacacata gttgacactt actgttgatc cttctgttat ttttgagtcc
     8401 cggcgtacaa taacgaatgg cgcattcaga taataggaca cagcttgggc aataggtacg
     8461 ccttttgtgg ctacagtcat tacagcatcg attttttcac caaggtattt agaagcaatg
     8521 attcttccaa cttgacgcag cagttcaggt tctcctaaca aatcagagag ataaacatat
     8581 cctcctggaa gcagacggtc ttgttctgat agacgttctg ctagagattc aatatatttt
     8641 ttcgcctctt catatgaaat ttcagggatg aacaatacac cacctgcagc acccgggatt
     8701 gtttccaagg ttccataccc tcgttcttta aatgttttct tgatgattcc caaatcttca
     8761 ctgatagaag atttagcaga ttcataacgt tcggcaaagc ttgttaagga aatcaattca
     8821 tgcgggtgat tcaataaata ctgtgtcatg tcaactagac gttcactgcg acgtactttc
     8881 acctattcca actcctttta agaaatttta tcgttttaca ttatataagt ttttcttcaa
     8941 aaattcgagc attttatgaa aaaataagaa aatagttcgg tatttattaa aataaaaaga
     9001 agcctataaa aaatttttcg tttttataag gcttcttttc tttataattt agttattttt
     9061 tcttctgaag gcaaccaata ttcgtttgat gatatttaca atcaggaaca aagcaataaa
     9121 gactaatgtg attgttgctc ctggaggtgt atctagataa aacgagctag ttagtcctga
     9181 cagcatccct agaaatccta cacaaacact gatagcaatc acgacattga agcttttgcc
     9241 taatcgcata ccgattgctg cgggaagaac catgattgcg gatatcaaaa gtgcaccggc
     9301 aatcgggatc atcacagcaa tagccacacc agtaatcaca ttgaacatca tcgacatcaa
     9361 atgaacgggt agcccatcca cgtgggcggt atcttcatca aaagtcagaa catacatcgg
     9421 ccgtttgaat aaaaagaaca atataccgat ggcaacgaat aagattccta aaaagatgac
     9481 ttgctcccat gtaatggtca cgatcgaacc aaacagatag gattggatac ttgttgctga
     9541 attccctccg ctcaaattca tcaatactaa cgctagcgcc aaaccaccag ccattaaaat
     9601 ggcaatggag atttctgaat aggttcggta gatcgttcgc agatactcca atatcacagc
     9661 tgctaataca acgactagca tggtcgtaag tgtaggatta aggttcaaaa agaatcctaa
     9721 cgctacccct gctaaagaga catgagaaag cgtatcagcc atcagcgatt gtcttctgat
     9781 cactaaaaac acacctagca tcggggcgat tccggcaatg aagatagcgg caagaaacgc
     9841 tcttctcatg aattcatatg aaagcaacgc cattgagaat cctcctttct caccagtcgg
     9901 atctgtctgt ccgcatactg tttgatatct tcatgatcat gggtgatcat tagaactgat
     9961 ttcccatgga catgcgcact atgtcggaga agacgataaa attcatttct ggattcttca
    10021 tccatacctg ttgttggttc atcaagaaca aataagtcag ggtcagtagc aaaaatacgc
    10081 gctaaactga ttcgctgctt ttgtccgcca gacaattcac ctattcgccg atttcgcata
    10141 tcccacatcc ctacagcttc taaagcacgc tgtacatgtt gatgatcttt ttcagagaga
    10201 cgcttgaacc agcggcctct tggatatctt cctgaacgga ctaattcgat caccgtacta
    10261 ggaaaacctg cattgaaaga tgcaacttgt tgaggaatat aaccgatact taattttttt
    10321 ccttcttgat ttttctcagc aatctttatc attccttttg aaggtttcag taatcctaac
    10381 gatgctttca ccaaggttga cttggctgct ccattttctc cagttaaaat gacaaattca
    10441 cccgcatcta catgataaga gacatcttct agtacaggtt catcatcata gtaaaaggtc
    10501 aaatcagata tttcgatata acgcacctta caacctccat tctaaaactt aattgatact
    10561 ttcttttaaa gctttcagat ttttttccat gacagaaata taattttcgc ctgctttgat
    10621 ttgttcatta gtcaagcttt ctaaaggatt caacacttct agcttcactc ctgtttcatt
    10681 cgacaatgtc tcagctacct tcgaagaggc attttcttca aaataaatca ctttgatgtt
    10741 gtttttcttc acaaattctt ttagctcagc taagcgactt ggtgtgggtt cctgatctgg
    10801 tgaaacgccc gaaatcgcta cttgttttaa gtcgtattga ttcgctaaat aagcaaaagc
    10861 agcatgctga gtgacaaatg tgcggtttgt tgcatctttt agctcatcgg agtatttttg
    10921 atccagtgct tcaagctttt tgatataagc atcggcattc ttcgtaaaag tttccttttt
    10981 atctggatat ttttcgccta attcttcagc aatcgttctg acttctttta tagctaatac
    11041 cggatcgagc caaacatgtg gatcgtattc atgtgaatga ccatgccctt cttctgattc
    11101 ttcatgttcg tctgcttctt gcgattctaa acggtcgatt cctttagctg cttcgatcat
    11161 caaggtctgt ttgctatcca ctgaatcctt taacgaatcc acaaaaaatt ccatgtctga
    11221 actattatac acgaaaacat ctgcatcgga aatttctgct ctttcttttg cacttggttc
    11281 gaaatcatgc ggctccgttc cagcagggat caaaagtttc acgtttcctt catcccctac
    11341 gatctctttt gtaaaatcat acatcggata aaaagtcgta acgatcgtca aatcctcttt
    11401 tttgtctgct tccttgttcg tatttccaca tgctgcaaac agtaaagcac ctatcattac
    11461 cgtagctgtc aaaagatatt ttttcattta tatgctcctt tcaatgaaca aatcggaatt
    11521 ttacgattta ttaacgtgaa ctaatgtatc aaaataaaaa aacacagtca atagaaatcc
    11581 atcaatttct gatttaagca tgtatattct tgattaaatc gtaaaatgga tgagttgctg
    11641 ctcatatcta gccatttcac tacaataaaa aactgaagaa taccatttta ggattcctca
    11701 gtttttcatc acctatttta cttcttatat tttattgcag ggttcgtact aaataaactt
    11761 cctcacaaaa accttttaac ccattatata ctctctgcgc tcttgaataa tgttgacaca
    11821 aggcaaaaac ggttggtccg cttccactca tcaatgcagc atctgctccg tacttgatca
    11881 tacgctcttt gatttgatgg atgactggat gccgctttcc cgttatatct tctaaactgt
    11941 ttcccatttt tttgatcatc tgctgataat cttgctctcg gatggcttca gcaagtccgt
    12001 ggatgtcttg atgttggatg gcttgcgtat ctacttctct aaacactgta cgtgtagaga
    12061 cactcattct tggtttgacc aacacaaccc agcactgtgg catagaaggt aatacttgga
    12121 tttcttcccc tttgccaccg acaaatgccg tacttccgta gacacagtaa ggaacatctg
    12181 taccgacttc caaaccgatt tctgccagtt cttctaacga acagcccaga ttccataatt
    12241 ggttgatccc tcttagagca gctgcacagt ctgtacttcc tccgcctaat ccagctgcta
    12301 ctggaatatt cttcttaaca aagatcttga caccggtttt gatattgtat ttatttttta
    12361 gcaataaagc agcttgataa acattgttcc gttcatctac cggtaaaaat gctttatttg
    12421 tttctacaat gattttgtct tctgttattt cttcaaaaaa tagatgatct gccaagtcta
    12481 cgctcgacat caccatttcc aactcatgat acccatcttg ccgtctatat aatacatcca
    12541 gtcctaaatt tatcttggct ggcgcttttt caatcaattc catcctctca cccattcgaa
    12601 aagtctaagt cgcaagtatt ctatcatagg gaaaaataaa agtatagctt atgtctgcct
    12661 agtggttgat tttgtcatcg tttcaacagc tttcacagaa ttttctcttc ataatccaaa
    12721 caagttctag tgattcttat aattctgtga gcacttcttc tgccaaactc ggaacatttc
    12781 ctttttcttt tcctatcaga ctgagtagct gcatggtagc aaatccgaaa ttacttcctt
    12841 tcctataagc aaaataacgt ccttcccaat gatcgacata ttttaatttc ttttcataag
    12901 cagactggaa ggaatagaaa aaattaccat aattatatac gatatgcatg actttttctt
    12961 gaaagaaaga aaaatctgtt tctccaacat tagctaatgg agctaagcct agattggctt
    13021 ccgagtagcc cattttctcg tatacaataa acagattggc taacagatag tcagctaatt
    13081 cctcagattc tttaggtaag acccgcaaca aatcatatcc cgcctgcttc ttcgtcatcg
    13141 gctgagcggt gatgaagcca acgacttttt gatctttacg gacaataccg acaccgctag
    13201 ataatacata tgatttatca aatcgtccca cagagaagta tttttctcgt gcaccttgaa
    13261 gccatttttg tgaaacttca tttacagctt gatgtaattc ttccggcagt tgatcgtacc
    13321 aagtaaaatg gtatccttct ttcatcaaac gttgatattc aagttgctga ctcagccgag
    13381 acaagtcttc tgttgctaga tcaacaatcc ctgcttcacc tactttcgtg aagtgaaaac
    13441 ccaattcatg aagcatcact acgtagcttt ctgatatctt ataaaacgca agctgatagc
    13501 ctaaacaatc tgcttgtttc ataaactgat tcgttgctgc ccgcatcatt ttaggattgc
    13561 caactggatc tcctaaaaca aagcatttgt ttcctttgat ctgataacca aaaacgactt
    13621 gatcttggtc gtcttcttgg taataaaaat aactgtaacc tgacaagcga agctgatggc
    13681 ttgtagctgt gcctccgtat gtttccaata aacggtcaaa acggtctttt tcgtactcac
    13741 tgccaagctt cgtagtatct tcagctaaat attgataaag cgccactaaa gctagcaatg
    13801 agatgcccaa accaataagc cctgagaacc acacatcatc tgaaggaaac aaaataaaat
    13861 gatttcctgt tattcgaccg actaaaaagc gtccgctgtg atagcctgca attgcgtaaa
    13921 cgataaacaa aaagccgaac aagctagcat caaagattat agctccccaa gaataaacga
    13981 attttgttcg ataaaattct tttctggata accacacagc taacagaatc aagacatata
    14041 ccaatatcaa ctgccaagat aacgtacgtg tgattgtatt taaaatacca aaagatagta
    14101 aagcaatcgt aggcagaaat gcctttttga cttttactga aagtgctcta gcaaggccga
    14161 gtaataaaaa gccaaccaat agattcactg tctgatctaa aaaattgaat gagaacggca
    14221 ataatacttg aaatagccga ctgattgcag acagattagt gattgttgat agaagtacca
    14281 tcatgatccc cgcaaaatag acagcactta cgagcacgat atgcgcaacc ttttggaaaa
    14341 acagtcgcgg aatgttatct aaaaatcgat tgattttgac tcctgtatgt gtgatgaaca
    14401 atagaagtcc cgtcataaaa ggtagaacat aataaaacaa ccgataatat aatagccaaa
    14461 ctactgctac atcttgccga acaggcaatt gactcattcc taagatcatc aaaacgtcaa
    14521 aagtccccat tccacctggg accatcgtca gcattcctat caatgtagca atcatgaaca
    14581 taggatagat cgaaagcatc gaaatatcta ttctcatcaa cttgccgatg atcaaaaata
    14641 ccgcaagggc tcccgtccat tgtccaaagg atgccagtag cagaccaagc aagccttttg
    14701 gataaaactc gaagaacaat gtctttctct ttagataagc aaaaaagaaa atgactggtg
    14761 ctaaaaaact accgccgaaa agccagatcc aatagcttcg aaaaatactt tctgactgaa
    14821 taaaaaagac atcaataaat gtaagaaacg accatacaga caaaccagtc agcataaata
    14881 gtgcgacttt tgatacagtg gctaaaacca tttttctatt tgtctgcttt ccataaaaac
    14941 tagcccgcag acttgctcct accacaccac caaaaccggc gagattgttg atcgtgtttg
    15001 ttacccacgc agcaagaaga aactcttttc tttccatttt tggttttcct tgtttttcta
    15061 agactctgat cgtcacccaa tcatacaaca acatcggagt gacacccagc agtccagcca
    15121 gaatcatgag caggatcgtc atgcggcttt gttgtcccat cctttgccaa atatcttgcc
    15181 aactcatgcc attagcaata tttgcgactt gatttgccac aaagactaac acggagccga
    15241 aaaaaatcag ttttaaaatc agactatgtt cctttatcca ttgcagcatc aagtatcgtt
    15301 tatttttcac attttcccat tcttaacttg tatacttttg tgtcataccc caatgttatc
    15361 ttatttattt taatttttcc actttagacc tgttctttgc ttgcgatgat agaaaaagaa
    15421 agatatccta atgctaaagg agaggataac atgaaagtag gatttatcgg cacagggaat
    15481 atggcacaag ctattattga tggtttatta aaaaaaggaa cagtctcgaa agaagaggtg
    15541 ttcgtttcca gtggacattt tgaaaatgca caaaaatttg ctgctgaaac tggcgtccat
    15601 gcatgcaaaa gtaatcagga agttgcatca caatcagata tcgttctttt agccgtgaaa
    15661 ccaaaaatca ttgaaaaagt attggaagaa ctaagagaat atagccagaa agtcttattc
    15721 atttctattg cttcaggaaa atcattaaca gatttagaaa cagcgcttga agatcctcag
    15781 gcctccatcg tacgtgtgat gccgaatgtc aatgtcagcg taggagcagg cgtttctgcg
    15841 atttgcaaaa atgaagctgc cacggaggaa cagcttcagc aagctacgat gatttttgaa
    15901 gcaatcggca gcgtctatcc tttagcagaa tcagatttca gtacattcat tggccttgct
    15961 ggcagctcac ccgcattcgt ttacatgctg atcgatgcga tgggacggac tggtgtactg
    16021 aatggtttgt ccaaacagac agccactgag attgctgcaa aagcaattat gggttcttgt
    16081 caaaaattcc tttcaagtga taaaaatcca tgggaacttg tagaccaagt ttcttctcct
    16141 ggcggtacaa ctgttgcagg tgtagttgcc ttagaagaag caggattcat cccagcaatc
    16201 atcaaaggaa tcaatacgac gatcgagaaa gatcttgaga tgttgaataa aaaataatga
    16261 tagcaatgtt ttttcgtgcc ttacaaaaaa agaagagctg tggcttttgc cgcggctctt
    16321 cttttgtatt ttttattatt tagcataatc agttgcacga gtttcccgaa taacgattac
    16381 tttgatgtgt ccaggataat ccagttcttc ttcgattcgt ttacgaatat cacgtactaa
    16441 acgaacagcg tctaaatccg agatttcttc tggcttgacc attacccgaa cttcgcgtcc
    16501 agcttgaact gcgtaacttg attctacacc ggcgaagctg ttcgaaatat tttccagact
    16561 tcttaaacga cggatgtagt tttctaatga ttcacttcgc gcaccaggtc ttgctgctga
    16621 gagcgcatct gctgcagcta caaggacaga gataacagaa gtagcttcca catctccatg
    16681 atgagaagcg atcgcgttga ttactactgg gttttctttg tacttggcag ctaattccgc
    16741 accgatctca acgtgagatc cttcaatctc atggtcaagt gctttcccaa tatcatggag
    16801 caacccggca cgttttgcta attggatatc ttcgccaagc tctgctgcta agataccagc
    16861 taattttgca acctcgaccg aatgcttcaa gacgttttgt ccataactag ttctgaatcg
    16921 cagacgtccg atgatcttga ttagatctgg atgaagtgta tgagcaccga cttcaaacgc
    16981 tgcctcttcc ccgtattcgc gaatacgttc atccatttct ttacgagatt tctcgaccat
    17041 ttcttcgata cgcgctggat ggattcgtcc atcttgtatc agtttttcca aggtcatccg
    17101 tgcgatttct cttcggattg gatcgaatcc ggaaagaaca actgcttccg gtgtatcatc
    17161 gatgattaaa tcgataccag tcagcgtctc aagcgttcga atattacgac cttctcgtcc
    17221 aatgatacga cctttcattt catcattcgg aagggtaacg actgaaacag tcgtttctga
    17281 aacttgatct gctgcgcagc gttgaatcgc taatgataat aagtttttcg ctttgcgatc
    17341 tgcttcttct ttggcacgct gttcagattc ttttaccatt agagttaact catgattcag
    17401 ctcttcttca gtcgatttca tgatgacatc tcgtgcgtcc tcttttgata aaccagcaat
    17461 tttttctagt tctttttgtt gatcttcaat cattttttca acgtcttttt ctcgctcttc
    17521 aattaattgc tgttttgaac taagtgcttc ttctttactt tcaagtgagt tctcgcgttt
    17581 ttctaacgag tcatctttac gattcagcgt ttgttcgcgt tgaatcaaac gattctcttg
    17641 agattttaat tctaacttac tttctctcag ctcactttca acttcagaac ggtatttctg
    17701 attttcttcc ttagcttcta acaatgcttc tttttttaga gtttctgctt ctttttttgc
    17761 actatcgatg attccagcag ctgatgattg tgcaccagct atttctttct cgtgacgtga
    17821 ctttgcgacc ataaacccta aaccaagacc gacaattaaa ccgatgatag cgaggagaat
    17881 gttgaatacc ataaaatcca cctccatact atctttttca tttttatcat ctatacagct
    17941 tctatgataa attattctaa actcgtcaat atgcatcttc gcatatatgt gtacaaaaaa
    18001 catgcacaac tttattctaa tgtttgtacc ccgtagtgtc aactttaaat aaacaaagaa
    18061 acggctttct acttattcat taaacgatgg tttttaaaat aaatgacact agtttcatat
    18121 aggtaaacct ttaaattgag aaacttttct attaaattat tctttgtaga tgaacagata
    18181 tttttatttt atgtacccac tcgtataatt aaataactca cgatttcttt attcaataaa
    18241 aaaacggcag gtcaaggaga gtacctactc ttcagaccag ccgtttttgt ttatcataat
    18301 ttttattctt ctactaaagg caattcttct tgtccttttt catcttctgg gacagcgact
    18361 tcttcaccaa taccataagc tgctctaact agagcagaaa cttctgccat catttcagga
    18421 tgattagcca tatagatttt agcgttttcc cgtccttgac caatccgatc ttctttataa
    18481 gaataccaag caccgctttt atcaacaatg tctttttcta ctgccatgtc tagaagttct
    18541 ccttcttgtg agatccctaa accatacatg acatctactt cagcgatttt gaaaggaggt
    18601 gctactttgt tttttacgac tttgatcttt gtacggttac caacaatatc tgtaccttgt
    18661 ttcaattgtt ctgcacgacg tacttccaaa cggatcgttg cgtagaattt caatgcgcgt
    18721 cctccaggag tgatttctgg attcccaaac atcacgccta ctttttcacg gatttggttg
    18781 atgaaaatag cgattgtttt tgttttgttg atcgaaccag agagtttacg caatgcttga
    18841 gacatcaaac gtgcttgtaa cccgacatgt gagtcaccca tttcgccgtc gatttctgct
    18901 cgtggaacta aagcagcaac tgaatcgact actacgatat ctacagcccc acttgatact
    18961 aaagcatcag cgatctctag accttgttct cctgtgtcag gctgtgaaag aagtagttca
    19021 tcgatattca cacctaattt ttgtgcatat tgcggatcta acgcatgctc agcatcaatg
    19081 aaagcggccg ttccgccatt tttttgtact tctgcaatag cgtgtagtgc aactgttgtt
    19141 ttacctgaac tttcaggtcc atatacttcg atgatacgtc cacgggggta accgccgaca
    19201 cctaaagcga catctaacgc aagagagcca cttggaattg tagagatttg ttggtcgatt
    19261 ttttcgccca atttcataat agagccttta ccatagctct tttcaatttt tttcagtgca
    19321 gcatctaagg ctgctttacg atcatctgcc aatacgataa tcctccttat atttacaaat
    19381 cagagcaaca tttcattatc cttatcatat cttttttagt gaaaaaaagc aagaaaaaac
    19441 cgaacaaata ttcgcattta tcattttctt aaaagctgtt ttctcaaaag atcaaatcct
    19501 tgcataaccg cattatatct gatagcagca cgatctctgg caaagtgata tttttccgaa
    19561 accacgccgt ctttagatga aatagcgatc catactgttc ctgcagattg accttctaac
    19621 gtgtccggtc cagccactcc ggtgaaagct acactgtaat ctgttcttgc tatctcacgt
    19681 gcttgaatgg ccatctgctc cacacattct ttgctgactg ttccatattc tgctaatagc
    19741 tccggatcaa tctccagaaa accagctttt gtttggagcg agtaggtaac gaatccgcct
    19801 ggaaatactt cagatacacc agcaatgtcg cctaatgcgg cttggaaggc gccggctgtc
    19861 aaactttccg ctgctgttac agtctgcttg ttttctttca gtagttccac tacgactttt
    19921 gctagcgaat tatcatcgcc gtatccataa aaatattccc ctactcgttc ttggattttt
    19981 tcttctaatg ctaaaagagc ttgattccct gcctgaacat catttgtttt cactgtcagt
    20041 cgtaatgtca cttcatttgg tttggcatat ggtgcgatcg ttggattgat ctgtgtctca
    20101 atcagatctt ttagctcagt gaccagtcgc gattcaccaa tcccataaaa gcgcaaaact
    20161 ttagaaatta gtttttcttc agaaggaaaa cgttcctcta gtaaaggacg tacttgttcc
    20221 acaaacattg gtttcaactc attaggcgga ccgggaagaa ggatatacgc atgtgtatct
    20281 gtttgataaa aagtccctaa agcaagccct gtacgatttg gtagaggaac cccgccttcg
    20341 ataatctggg actgttgcag attgtttttg gtcatttttc gatgggttgt ttcaaaatat
    20401 gccaataatt ttttgtatcc ttccgtattt tgtatgagag acttaccgag gtgagccgca
    20461 gttgcttctt ttgtcaggtc atcttctgtc ggtcctaatc cgccacataa gagtatgagt
    20521 tcacttcttg tttcagctag agcaatcaac tcttctaaac gctgctgatt atctcctaca
    20581 actgtttgat aataaacttc gatacctaga tcagctagct gttctgacaa gaaagtggca
    20641 ttcgtattca cgacttgtcc tagtaaaagt tctgttccga cagctattat ttctgctttc
    20701 attacttccc gctcccttat atagtatacg ctgtattttt atattttttt atttaaaaag
    20761 agggcaagga gctgtgacaa aaatcttgtc acagctcctt gttccaaata aacagtgttc
    20821 tcctgccaaa acctcggtca taagtcacaa tatacataaa acatgaaaag ctgttttttg
    20881 aaattatccc ttgtgactca gagttagacg gcaggctcac aacctctcca ctaaacgata
    20941 cttacattga tcctttgaac acaccgctgt ttttgatgaa gtaatccaca cctgaataga
    21001 tagtaaagat cagacaagca taaagcatga tttgatctac tggaataccg atcaggctga
    21061 atgggatatt attgataaac agcaagataa tagcaaccat ttgtgtagcc gtcttgactt
    21121 ttcccggcca agctgcagcc atgacttctc catgttcgac aagtagtaaa cgcaagcctg
    21181 tgacggctaa ttcacgacag acaatgatcg ctactaccca accaggggcc tttccttggt
    21241 caaccaacat gataaaagca gtcataacta gcattttatc tgccaacggg tccgcaaact
    21301 tgccaaaatt agtgacaagg cctcgcgcac gggcaatttt tccatctaac cagtctgtga
    21361 tactagctaa agcaaaaata actgcaccta ctagctgtgt gacagccagt gtggtgtctc
    21421 ctacgttaaa ttgtccccaa tctaaaggta aacctacaac caaaatgaaa atcgggatca
    21481 tacaaattct aagtacagtt aatttatttg gtaaattcac actatcgact cctttcttcc
    21541 ctattctacg tgactatcag cgaagtttcc accgatatta tttacttttc caaatgaatt
    21601 tattcaaagg agtattttta ttgaaattga tctttttgaa ttaacgaaga aggaccgcga
    21661 cacagatttg tcacgatcct tcttttttat acctacaagt aaacagatgc gtaaactaat
    21721 ttatacgatc attctgcgta ttgaagagta aggttcacat tttttggact ctgattgttt
    21781 tcccctggat taactggtac ggattctcca ttcgctttga tcgttgcatt gcttgctgca
    21841 ccaatcgtga tggtcgcatt tgttgctgtt tccggtaaca cggtactctg tgtttcattt
    21901 gctgccagtg tcccttgata aacataggca ttgtctacta gcacacctaa ccatacacga
    21961 ttgacagcag taaattcaag ggttacaggc ttggtagcgt ctgtgatatt tactgcgatg
    22021 gcactgccag aatcttctcc tgtagctatt gccattttct tttcttcact tgattttgtt
    22081 gttgattcta ctgtagaaga ctctttggtc tgcgtgcttt gctctgagga agaggactcc
    22141 tcgactgatc cttctacttt aagcgaagaa gctgtgtctc cgattattgg atcagcttga
    22201 cgatcttgcc aagtcatata gcccacgaca acgacaatcg ccaaagcaac caatcctaat
    22261 aaaatgacag gaagacttgt ccaaaagcga cttggatgcg attcttcctg atgcattgct
    22321 tttctagaag tacttactgt ttcaggctgc ggacgttcag ggaccgatcc tgtcaatttg
    22381 cttttcccat caaaaacatc gactaaatgg tctccatctt ctcctacagc ttcagcgtat
    22441 tgccgaataa atgcccgcac gtaaaattta ccaggcaatt ggtcaaaatc atcattgtcg
    22501 attgcttcca aataacgttt ttgaattttt gtaatctgct gtaattcatc taaagatatt
    22561 tttttattca ggcgtgcttg ccgtaatttt tctccgatgg tttcaaaagc cactcgttca
    22621 tcttctccag tctgtttgat tctccgaagc atctgctgtt gcaacgaaag acgttatgtt
    22681 tgggtgctgt gacaaaagtc atgtcacagc tccttttttc cgaataaaca ttgttctcct
    22741 gccaactcct cggtcacaag tcacaatatc caataaatat aagaagctgt tttttgaact
    22801 tgttccttgt gactcggagt taaacggcag gctcacgacc tctttcttaa acggtgttca
    22861 atcagctgtt cctcggtatt agctcaaaaa ccggcaaaat ataaggagat atttttccgt
    22921 atgtttctct aatactcagg tcaaaacgct gatttcacaa cctcttcctt aaacggtgtt
    22981 caaaagcttg catctgcggt aataagtcca accaagttaa aaacattaag aagctgtttt
    23041 tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct tggtacacgg
    23101 tgttccagaa gtagcttctt cggcaataag ccgaaatatc acaaaaattt gagaagcaat
    23161 tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tctttgatta
    23221 cttcgtttga ttttatttta atataagttg attaaaagag agcatgattt gttttatttt
    23281 ctcactaatt tttccaacac aactaagaat accataaaag ctgattttgg aggaggttcc
    23341 tactacaagt ctaaaacgaa atttaagact tttcctcata aaatactggt ttattttggt
    23401 ctcatgtaaa actcgctgaa ggcctcttca cttataaatg cttccccagc tgccagtacg
    23461 tctttcattt ggatcgaatc gatgatttca ggaagatcaa ataaagttcg atcgccaaat
    23521 aaactttgcg taaattgatt ggcaatatat tcgatcgaat ttaatgattg gaaatattgt
    23581 ccaagcattt tcttcttcag caaatcgaga tttgtttctg acacttccgg atcatcagcg
    23641 aatccaagaa tgatttcttt gactttttct gctgctttct ctggttcgtc cgtatctccg
    23701 ctgaagtccg caaagtgaaa ttcacgatcc aagctgaatt caaaaccaaa gctgtcatcg
    23761 ataattcctt gattgtacat cgctagatag tttcgagaag tattgcctaa aatcatttgg
    23821 aacaacagat tgattgctgt tttatagcgt aacagttctg ttccttcttg cggcaatgtg
    23881 tctaatcctt taataccaag tacaaattta tctcgtgtaa tggctgcttc caatgcactt
    23941 tgtttgatga tttcttttgt gttttcaggg aaataacgta caatttcttg ctttggaggg
    24001 aaattttttg cttcttggtt ttcccgaata agtttcatca atttctcagg ctccatctta
    24061 ccaactacaa ataaaaccat attgcttggc tgataaaacg tccgatagca cgtatacaag
    24121 tcttgagctg tgattttgtc gatactttca accgtgcccg caatatcgat atgcaatggg
    24181 tgtgtaggat acaagttatt tagtatgcca aaaaacatcc gccagttagg gtcgtcttca
    24241 tacatttgga tttcctgacc gatgattcct ttttccttgt ttacagtttc ttctgtgaag
    24301 taaggtgctt gtacaaaatc gatcagagtc gtcagattct tttctacctg atccgtcgta
    24361 gaaaacagat agctggtttt cgtaaaactt gtgaaagcat tggcggaagc tccttgtttc
    24421 ccaaataatt gaaagacatc accatcttct ttttcaaaca acttatgttc taaaaaatgg
    24481 gcaattccat ctggaacttt caccttttct ttttcaccat agggaataaa ttcgttatcg
    24541 atcgaaccat aattcgtact gaacaaacca tatgtcttgt gatagtcatt tttaggcaac
    24601 aaatacacgg tcaaaccgtt tggcagtact tcatgataaa gtgtttcatt gatttgctca
    24661 tattctgttt tattcatttg catttcctcc tgctaaaaag aagattgcct gcaattcgat
    24721 ttgctcagct acttgttgta tctctttgat cgttacgctc tccatacgtt tcaaccattc
    24781 ttcatcagat aattttgttt ccggcagcca gctgtccaaa aaagaagctt cgattgccgc
    24841 ttgcggagag tctagagaaa gcagatattg gtttctcagc attgcttttg tctgagacag
    24901 ttccaactct gttatttcac catttcgcaa actttctaat tgctcgtgaa tcagatgaag
    24961 tacttgattt cggttctttt catcgatacc ggtttggaca ctcatgaatc ctcggaacgt
    25021 atccacgcta ctagaggcat aataagctag actttctttc tctctgacat tcatgaaaag
    25081 tttagagtga ggaaaaccgc caaataaacc gttaaagacc attagcgcaa aacgttctgg
    25141 ttcatcgtaa tacacatttg tctgataagc taggttcagc tttgcttgaa cgattggctc
    25201 acgtacttgt cgttccttca cgatattcac ttgaggttgg gtataaaaca gatctggata
    25261 aactgtttcc cgcgactcaa atggtaattg cccaactgct tcagctgctt tgtccggatc
    25321 aacatcgccg acaacaaaaa tgtcgatttg atcatttgcc atcatttctt gatagtagga
    25381 cgctagcgaa ctagcagtga tcttgtccaa atcttcgact acgcccaacc ctgggatctt
    25441 ttgttccggg ctgttttcaa aataaagttc ttgcaaggca taggaagcaa aagtttgttt
    25501 atcttccttc aaactttcca aatatagacg cagattgttc ttttccaagt caaacgtcaa
    25561 ctgatcaaac tgatgatttt taatatttgg ataaaataaa acctctttta aaaaatctac
    25621 cgcttgagaa aaaaggtttg gatcattgac atattcacca ttcacaaaac tgatccctgc
    25681 gtttagccaa tgtaaatttc ctttttttct aactgataat ccaaagcttg cgccataaag
    25741 ctctgcaagt tgcgcactta actttgtctg atccggataa ttcaaactat ttgtttccaa
    25801 catgctgctg agaagtgaac gtttcgtgat gacttcttgt tgcagacgcg cagtaaaacg
    25861 gataaaaatc cggatcgttt tgtattgttt tgttggtaag acatgaagat tcacgccttc
    25921 tgccaattga tacaccatat ttatatgctc cttttcacag ttgattgccc gcttactctt
    25981 ttaatgggtc aacctgcctt ttttacttat tcctctgctt gaaatttata agttcaggca
    26041 attcgccgtt ttttctaaat gatgttacac tttatagtat agcatagatg ttttttatca
    26101 aaagaaacag acggctaaaa gcgtatattg tttttttatt ttttattttt cgatatacta
    26161 aagacaaata aaagaaggga gggtcattca tgatcaaaga attcaaagag ttcatcatgc
    26221 gtggagatgt cttggatctt gctgtcggcg ttgttattgg tagtgcgttt acaagcatcg
    26281 taaaccaagt ggtgcaaggc ttgatcacac cactaatagg ttggtttgtc gcaatgatca
    26341 caggtacttc tgatttagaa ggagcacttt caattcttga ctggtcgccg acaaaaggcg
    26401 tcacatttgc gtttggtaac gttattagtg ctatcattac atttctgatc actggttttg
    26461 tcttattctt agttgtcaaa gcggcaaacc gtgcgaagaa tttacgctcc aaagaagagc
    26521 aagtggaagt ggaagaaact cctactgcag aagattatct tagtgacata cgcgatcttc
    26581 ttgccaaaca agtggcagaa gacaatacac atccagtaga gcaaacaaat ctttccgata
    26641 cttctaaata ataaagaatc taaaaaaaag aaagccgatt ttcagtgtcg gctttctttt
    26701 tttagattct taacttgcta cttgttcctt tttcatgtcg atatgtggaa tatcatcttc
    26761 caaatacaca tctgagattg ccttgaatcc aaacgactca taaaaagctt gtaggtattc
    26821 ttgtgcgccg atcacgattg gtttttctgg aaaatttttc tggatcatgt taatcgtttc
    26881 ttgaacaatc tttcttccta aaccatcttt acggttgtcc tgatgaacaa ggacacgtcc
    26941 aaaatggata ctctccgctt cttcatatac tcgcgtgtag gcaaggatct tcccatcagt
    27001 atcttgaaaa aacgtatgcc aagcttgtcc atcaatgtca tcaacttctt gataaggaca
    27061 ctgttgttcc acaacaaaaa ctgcgattcg ttctttgacg atcgcgtaaa actctgctgt
    27121 tgacaattct tcaaatttct ttacctgata ttccactaag caaacgcccc ctgctttttt
    27181 attcttagca taccgattcg cacacaaaaa agcaaacgaa ccttacgaat ctcaggctcg
    27241 tttgcttttt aaactgcttc agttaaaaat tcgatctcaa ctgtccgagt aagtacatct
    27301 gaatagctgt aagatactcg ttcaaaagaa ttttcttcag gatccaaatc aacaacgaat
    27361 actgaaggat aggtttctgt taaaacgccg cggcgttctg tttgacgctt tcttcctgtc
    27421 tgagcaacca acatgattgg gctgccgata cgaccttcta aatctttctt gattgaagct
    27481 aaagttgttg gcatacagtt cacctctact atcaactata ccacatattt ttaaaatttt
    27541 caagtttacc ataaaaaaaa aagaaatgca ataaaaataa ataagaagct cattaagatt
    27601 tttctaaatt attcctcatt ataaaatttc aagtgttttt ttattttgag ttttactctc
    27661 ccgtaagcgc tcattacttg cttgtgatcc attgctaaac attcagccat gtctacaaaa
    27721 tcaacgcctt gcatataata atgaaaaatt ttagcttcta tttttgaaaa ctggatttcg
    27781 caattttcga ttgcttcttt taataaaaga tgctgaatcg ctaaaggttc ttgcaatgtg
    27841 atctctgcta aaaagtctgg tccttctgtc tctatttttt ggtcgattga gacttcatct
    27901 ttttgatcac gacgcttcag cgcgttctgc ttcctcaaca gagaatagat ttgattgctt
    27961 ataatgcttt tataataact tccaaagcta acacctttgc ttttttcgta agttctcaat
    28021 gcctgatata aagcgatcct tgcttcttgt tcccaatcct cagaaacata atttcttaaa
    28081 taataggtgt ttttcaaacg tagtaaaagt ggaaaatatt tttgatacag ggcagcaaat
    28141 gcttcttcgt caccattacg tgcagcgcgc aataactcct cagacataaa ataattcctc
    28201 cataaattcg tttatggagg aatcttatca tcaatgattc atcgaactga ttagaacgat
    28261 atgttattta ttttttctca ttaaatcgtc caatttttgg gacaattttt ttaactgctc
    28321 ttcattccat ggagaattac gtcggaaatc aataaaatgc atatctttat tttcttgggc
    28381 tattgttttt tccgtttttt ttatcgtctt atataattca taagcagaag ttcgtaatgc
    28441 tccttgggaa aagatcatcc actgttctgc taaatcgctt gtcgccactg tcacttgtgt
    28501 caaacggtca ttcagctgac cagcgattct ctcgatataa ctgtcagccg tttcgtcttc
    28561 tttcgtaaaa acaacatcca actggtattt cttataattt tgttggatgc ccggaactag
    28621 ttgggcatcg aagacaacga tgacttccat tccttcatat ttcgcataat ttgataaacg
    28681 atgtaaaaga gcttctcttg catccgccat tttgtcctgc tttttaagct ggacaagttc
    28741 cggccacgaa ccaatcatgt tgtagccgtc tacaatcaac agctgttttt tcatggtcta
    28801 ctccccctga cgttgggaaa aagcctgata cataagcaaa ccagcagcaa cactggcatt
    28861 taagctctgg acgtgcccgg tcatcgggat cgtcagcaat tcatccattt cttttttcaa
    28921 tcctggactc attcctttgc cttcattacc aatgactaaa gcaattgatc ctttggcatt
    28981 ccatttttgg aagtttgtgc cggtcatatc tgtgccaaat acccaaaatc ctgtttcttt
    29041 gagctgttgc acagcttgta ccaaattcgt cacgcgtgca acagggacat gttcgactgc
    29101 cccagtggaa gtctttgtta cgataggtgt gattccaact gctcggtgtt ttggtatgat
    29161 cacaccatcg acgccacttg cgtctgctgt ccgtaaaatc gacccaaaat tgtgtggatc
    29221 ttcgatgcta tccaaaatca aaaagaaggg gtcttctttt ttctcttttg ttcgttcaat
    29281 cagttggtca agggataaat attcataagg tgtaatcgca agaacgactc cttgatgcac
    29341 gccgtgatcg ctcaaagttt ctaatttttg tttaggaacc catttgactg gcacagcttg
    29401 ttcacgtgcg atttctttca gttcttctac tttttctcct cgtgaatctt cttgaagaaa
    29461 gagtttattc cctcgccctt gttgtagcgc ttctactgcg gcatggtgcc caaagacgaa
    29521 attatcttct aatgcttctt cattttgatc agaaactgac tgatttcgtc cagtttgctt
    29581 tttatttgaa tatctatttt gttttttgtc ttcaaatcca cgtttcgatt gatgtttgtt
    29641 tgttctttct ttacccattt ttttcaccaa ctttctgaat acaccagcgg atcaattctt
    29701 ccatccgctc ggtttgtttt gtcaaatgca gatagcccat gagcgcttcg aaccctgttg
    29761 cgacccgata cgtcgtaaca tcagcatttt ttgcgctcgt atggctttta gcatttcttc
    29821 ctctgcgata ataaagttct tcttcttctg tcaaaagatg ttcttccagc atttgctgaa
    29881 tcaaaaaggc ttgtgcattc gcagaaacat aattcgttgc cgcacgatgc agcttattgg
    29941 gttttgtttc tccttgatca atcaggtggt cacgaacata gatttcgtaa atcgcatctc
    30001 caacataagc caaggctaaa ccgttcaatt gtgtgtaatc tctcatgctt ctcttctcca
    30061 tcgggttcct tgcggtgtat cttctaataa gatccctttt tctttcaaca aatcacgaat
    30121 ttcgtcgctt cggacaaaat cacgattgct tcttgcttga tttcgttctt cgatcaattg
    30181 ttcaatctct tcatctaata actcttctgc tgtaaataaa ataccgaaga tcgaaagcca
    30241 ttgcgagaat aaagcttctc cttgtgccaa gacagcggca gaaactttcg gctggtctag
    30301 ataagtgttc agccacttag caagctcata gaccactgtg atcccatttg ctgcattaaa
    30361 atcatcgtcc atttcttcga tgaatcgctg ttctaaattt tctaacaaag cgattttatc
    30421 cgcatcatct gccaaattct ctgaggcagt ttcttggcgg aatttcaaat tgtcaaacgt
    30481 attcttcagt cgttgtaaat tggtggctgc ttctttcatc gtcgtttcac tgtagcggat
    30541 cggacgacgg tattgtgtgg tcgccatgaa gaaacgcaag acttgtggat caacgttttt
    30601 gatcatttca tgtacagtga tgaaattccc tagagattta ctcatttttt catcgtcttc
    30661 gccaatcgtt acatagccat tgtgcatcca gtaattagcg aattttttcc ctgttttcgc
    30721 ttcactttgt gcgatttcat tttcatgatg aggaaattct aaatcttgcc cgccaccatg
    30781 gatatcgatc gtttctccta aatgttttgt tgccataacg gaacattcaa tatgccaacc
    30841 gggacgtcct ttgccccatg gtgaatccca agaaatctcg ccaggttttg cttgtttcca
    30901 taaagcgaaa tctaatgggt cttctttttg ctgctgttct tcccctgtac gctgacttgc
    30961 tccgacttcc agttcatcga tggattggtc acttaatttc ccataatttt caaatttacg
    31021 ggtacggtaa tacacatcac ctgaagattc ataagcatag cctttttcaa ccaacacttg
    31081 aataaatgct aaaatatctg gcatatgatc catgactctt ggatgcagtg tagcaggctc
    31141 gacattcaac gctttcgtat cttcttcaaa tgctcggatg aaacgatcag caacttctgg
    31201 tgctgtaata cctaattcgt ttgctgtacg gatgatttta tcgtctacat ccgtgaaatt
    31261 cgatacgaaa tcgactttat agcctcgata ttcaaaatag cgtcgaattg tatcgaaagc
    31321 aatggtgcta cgagcatttc cgatatggat ataattatac acagtcggtc cgcagacata
    31381 cataccgaca tgtccctttt gtatagggat gaattcttct ttttttctgg tcaatgtatt
    31441 gtagattttt atcattctga tctcctcact ttttctcctt tgattcgaac gacttttgct
    31501 ggtactccta ccgctgtagc gtcgtctgga acatctttca acacaacggc acctgcaccg
    31561 attttggcgt tttttccgat cgttaccggt cctaatatct gcgcattcgc agagagcatg
    31621 gctccttgtt taacagttgg gtgtctttta cccgtttctt ttcctgtgcc gcctaatgtt
    31681 acgccatgga ataaaatcac atcgtcctcg atttctgcgg tttcgccaat gacgattccc
    31741 attccatggt cgatgaacac gccagttcca atcgttgctc ctggatggat ctcaacacca
    31801 gttaggaaac gccagaattg agcattgatc ttcgccaata aaaagagccg gtgctgatat
    31861 aaaaaatgag agaaacgatg ccaaaacaag gcatgaaaac cagggtatgt caaaacgatc
    31921 tcagctgtcg aacgtgcagc cggatcgttt ctctttgctg catcaatcgc tcgggtaaac
    31981 caactcatga ctttccactc ctttcgtaag aattttatgt aggaaatcat cttcttataa
    32041 agaaaagaag cgccttttaa ataagtttcc ttatcaaaag acgctttccg cgtggttcca
    32101 cttttcttca taatcaatga ttatctctga cagctaacgc ctgttatacg ttcttggcta
    32161 ctttgtttcc tataaaacag tttttcctgt tttctgtgtt tcgccaagac cactcccaga
    32221 ggcatttcgg caaccattca ttgatacctt gcaccacccg gtatctctct tgtaatgatc
    32281 agctgcttac ttttttctgt tcatcgtgtt tagttcagta cttgatttaa atgtgctaat
    32341 gatttttcac gaccaagcaa ttcgatcgtt tcaggcaatt ctggaccatg catttgtcct
    32401 gatacagcta cacggattgg catgaaaaga ttttttcctt ttacgccagt ttctttttga
    32461 acagctttga tggcagcttt gacagttgga gcatctacta cttccatttg ttctagctgt
    32521 gctttgaatg cgtggagaac agtaggaact gtttcaccag caagtacttc ttttgctgct
    32581 tcattcaata caggatgttc attgaaaaat agcgtagaca gatcaacgat ttctgcagca
    32641 tagctcattt gaggttgata caggctaacg atcttcttca cccattcgat tgtcttttca
    32701 tctgggtttt ctgctacacg tccatcagcg attagataag gcagacacat gtcagtaaat
    32761 gcatctagat ccattgcttt gatgtaatgg ttgtttaccc attgcagttt tttcgcatcg
    32821 aaagctgcgg gtgatttact taggcgtttt ggatcgaaca ttttgatcaa ttcttggtgt
    32881 tcgaagattt cgtcttcgcc cacaggtgac cagcccaata atgcaataaa gttgaacatt
    32941 gcatctggta gatagcctaa ttcgcgatat tgttcgataa attgcaagat tgattcgtca
    33001 cgtttgctta gttttttacc tgtttcactg ttgatgatca atgtcatatg tccgaaggtt
    33061 ggtggtgtcc agccaaatgc ttcatagatc atcagttgtt ttggtgtatt ggcaatatgg
    33121 tcatctccac gtaaaacgtg agtgattttc atcaaatgat catcgaccgc aacagcaaaa
    33181 ttataagttg gcataccatc gcgtttttgt atgacaaagt cgccaccgat attatcggat
    33241 tcgaacgaaa tagcgccttt caccatatct gtaaatgcgt attctgtatt gcgaggcaca
    33301 cggaaacgta caacagattc tagaccttgt gcttcttttt ctgcttgttc ttcaggtgtc
    33361 aagttcgcac atgttcctgc gtaatgaggc atttctcctc ttgcgcgttg tgcttcccgt
    33421 tctgcttcta attcttcttc tgtgcaatag catttgtatg ctcgattgct agcaagcagc
    33481 tgatcaatca gcggttgata gatttcttta cgttcagatt gacggtaagg gccgtattct
    33541 ccaggatttt caggagattc atcccaattc attcccaacc aagcaaggtt ttccaactgg
    33601 cttttttcgc catcttcgat attacgtttt tggtcagtat cctcaatacg aataataaag
    33661 tctccatcat tgtggcgggc aaacaaataa ttgaataatg cagttcttgc atttccaata
    33721 tgcaggtgcc cagttggact tggtgcatag cgtacgcgta ctttcgtcat tttcttcctc
    33781 ttctttcaaa ctagactttg actaacagca aaaattccgt cagttacggc ttctcaaata
    33841 tcatttattt aaagccactc agcacaaatt ttaccttttc taaggtaaat agtaaagcca
    33901 actggttgaa tttccttacc agttggctga atttcttttt tattttgatt gtttagtctt
    33961 tttctctgtc tcttttttat tgtcgattcc tcgtcctgca tgtgcaggtt tagcaaagat
    34021 catgcgtcca gcagctgttt gaagggcact tgttacaacg acttcgatac gttcattcat
    34081 gtaatgctgt ccatcttcta cgacaaccat cgtgccgtca tcaaggtagg ctactccttg
    34141 ttggcgttct gtcccggctt ttactactag aacattcatc gtttcaccag ggatgacgac
    34201 tggttttacg gcatttgcca acgcattgat attaagaaca ggtacatttt ggaattcaga
    34261 gactttattc aagttatagt cattggttac aatgacacca tctaaaagct tagccaattt
    34321 gatcaatttg ctgtctactt cactgatatc ttcaaagtct ccatcgtaca tttcgacaga
    34381 tataccctct tctttttgca aggcatttag gatatctaat ccgcgacgac cacgcacacg
    34441 tttcaaacta tcccctgagt ccgcgatgta ttgtagttcg taaagaacaa aattagggat
    34501 catcaaggtt ccttctaaga atccagtctt tgtaatatca taaatccgac cgtcaatgat
    34561 cacgcttgta tccaagattt tatatttatg gaaattttct cctgattttc gttctaacag
    34621 ttgactctca acttgactag ctgtttcttg ctcgattttt ttgcttcgtg aaccaaaaac
    34681 tttcttccat tcttcgattc gtgtcgttcc cattctcaat cccagatagc ctaagatgat
    34741 catcagtaaa atcggcaata cactattgac gaatggaatt tccatattgt aaagaggggt
    34801 cgagatcaca acacctaaag tcaatccgat gatcgcaccg atcgcaccga agagaagata
    34861 agtcaagctg acctcactta gtttttgttc tacttgtttg ataatagctg agatatgttt
    34921 ggccaacagc aaagataata aaaagaaaat aagtgcgcct aacaggctgt tggtaaatgt
    34981 attattcagc catccgtttg actgttgacc gattgcctgc caagctgagg gtaaaaatga
    35041 aatacccaag cttgcgccga caatgatcat cagcaaagta atgacacgtt tttgcatcgt
    35101 ttttcctccg tttcgacaat catttattag ttagcggaac acacgcttca acgtttcagc
    35161 caaagttgct actccaacta tctcgattcc ttttggcgct tcccagttac ctaagttgtt
    35221 tttaggtagg tatacttttg tgaagccgag tttttggact tctttgactc gttgttcaat
    35281 atgactgaca cgtctgattt ctccagtcag tccaatttca ccgatgaaac attccgttgg
    35341 ctgtgttcct ttttctttat agcttgaagc aatactgaca gcgatagcta aatcaatcgc
    35401 tggttcattt aattttaccc cacctgcagc ttttaagtaa gcatcttgat tctgaagaag
    35461 caacccagct cttttttcca gcactgccat gatcagagat acccgattga aatcaagacc
    35521 tgttgttgtt cgtttggcat ttccgaacat agtaggtgtg actaatgctt ggatctctac
    35581 caaaattgga cgtgtccctt ccattgccac aacgatagca gagccagttg caccgtctag
    35641 gcgttcttct aagaaaatct gtgaaggatt agcgacttct tccaatccat gctcatgcat
    35701 ttcaaaaatc ccaatctcat tcgttgagcc aaatcggttt tttactgctc gcaaaatccg
    35761 aaaactatga tgttgttcac cttcaaaata taacaccgta tccaccatat gttctaacat
    35821 tcttgggccg gcgatcgagc cttctttggt aacatgaccg acgataaaaa tggctatccc
    35881 atttgtttta gcgattttga gtagttctgc agtggtttct cttacttggc tgacacttcc
    35941 tgccacactt gtgatgtctg gctgcgtcat cgtttggatg gaatcaatga taacataatc
    36001 aggggagata tgttcgatgg cacgactgat ttcgttcata tctgtttcag cgtatagata
    36061 aaattctgtg ttgatacttc cgaggcgttc cgcgcgtaat tttatctgct ctgcgctttc
    36121 ttcgccggat acatataaga cttttccgcc tatggctgct aattgctgag aaacttgtaa
    36181 cagcaaagtg gatttcccga tacctggatc gccaccaatc aagaccatag accctggcac
    36241 cacacctccg cctaacacac ggttcagttc ttctaatttt gttttgatcc gaggttcttt
    36301 tttagggacg acgtctgcga tttttgttgg ttttgctttt tctcctgtca aacttgtccg
    36361 agttctgcgg tccgttgtat cttgttcgat ttcttcgacc atcgtattcc attttccgca
    36421 atttgggcag cgtcccaaga acttaggcga cacatacccg caattttggc aaacaaattg
    36481 tacttttgat ttttttgcca ttgtaccgcc tccttttatc ctaatcatga gacattaaat
    36541 caagcttaag tatctttaac gttttattta ctggaagaac caaatccgcc ttgacgttga
    36601 gcagtcgttt gatcttgatc tgcttttaaa aacggcaaaa agatcccttg gccgatacgc
    36661 tcgccttttt tgatcactac atccttgaaa ccaaagttgg tgaattggaa catgatatgg
    36721 ccttcatttg cctcattatt gtaataatca ctgtcaatca cacctactcc gtttgtcaac
    36781 actaaaaacc gcttcaacgg attgcttgaa cggttagcaa gctgcaaaaa ttcatcttct
    36841 cccatgtaag ctttgattcc agtaggcacg agggttggtt tcaattgttt ttcattttct
    36901 gatacatctt ttttcaatcc atctgcgact gtttccaacc cctttttcca gcctgctttc
    36961 cagatacttg gaatcacgac ctcttctgct gcttcaaaat catagccagc agcatgatgt
    37021 gtagcgcgct gcggtagatt gatattttga tctttatatt tagaaataac ttcaaatcct
    37081 ctttttctca ccggttttcc tccctaaata aactactcct attctaacat atccgaggct
    37141 gtaaaagact ttctttacaa attcttcaag aaattctgct atcttaaagt aaaccattaa
    37201 ttcgttcttt ttccaaacga ctgcaagttg tacaaaaaaa ctatttttta catgacttta
    37261 ctcgtcaaaa caagtagaat agaacgaaat taatgagtgg atatttgatt gaggtgtcac
    37321 atgcgtaaag aaattaaaaa tgcaaaacga attattgtca aagtaggaac aagtacattg
    37381 atttatccca atggaaatat caatcttgcg gcaatcgatc agttagcttt tgtcctttct
    37441 gatcttagaa atcggggaaa ggaagtgatc ttagtctcat caggagctgt aggcgttggt
    37501 atgcataaat tacatttgtc agaacgtccg actttgattc ctgaacagca agctgcggcg
    37561 gctgttggtc aagccgaact gatgaatatc tataatcaaa gattcagtgt atataatcaa
    37621 cagacggcac agatcctcgt gacaagagac gtggtagatt atcctgaaag tcgtaaaaat
    37681 gtgaggaata cattggaaca gcttttgatt atggacacga tccctatcgt gaacgaaaac
    37741 gatactgtag cagtggatga attagatcat ctgacaaaat ttggcgataa tgatcagctg
    37801 tcagccatca tggcaaaact tgtcgcggca gaccttttga tcgtcctttc tgatatcgac
    37861 gggttttatt cagataatcc gactacaaat cccgaggctc agatgtatcg ctatatcgat
    37921 aaaatcgatg aaaagattct ggcacaagca ggtggtgctg gtagcacatt tggaacaggc
    37981 ggtatgcaga gtaaattaaa agccgcttct aggatatttg aagcaaacag cgcaatgatt
    38041 ttagcaaatg gaaaaaatcc ggcaattatt tttgatattc ttgcaggaaa agaagtaggt
    38101 actttattta aggaggactt gaaatgactg acttgatgca attaggacaa caggcaaaag
    38161 atagcgcgca acaactagca cttatgccta caaaccgcaa aaatgagcta cttgttcaaa
    38221 tggcacaaac catcaaaaag aatcaacaag cgatcattga agcaaatcat aaagacttag
    38281 aaaaagcagt cgaaaacaat atcagtgaaa cgatgcaaga ccgcttgcag ctgacacctg
    38341 agcgaatcaa tgccatggct acagagatcg aaaaaattgc ttcgttagac gatcctattg
    38401 gaaaagtaga tgagatgtgg acgaacaccg acggattgag aatcggcaaa aagcgcgtgc
    38461 cactaggcgt gaccggcatc atttacgaat ctcgtccaaa tgtcacgaca gatgcagcta
    38521 gtctagcatt caaatcaggg aatgccgtta tccttcgcgg tggcaaagaa gcattcttct
    38581 cgaatcaatt attagtccaa ttattgcagc aagtactggt cagtgctggc gaatctcctt
    38641 atgccatcca gtttgtcgat gatacttctc atgaaactgc aggacagttg atgcgattga
    38701 cagaatactt agatgtgctg attcctcgag gcggagcaaa attaatccaa cgagtcaaag
    38761 aacaggcaac ggtacctatc attgaaacag gtacaggaaa taatcacgta tatatcgata
    38821 aagaagcaca gttatctatg gcagtgaaca tcatcgtcaa tgcaaaagct tcccgtccat
    38881 ctgtctgtaa cgcggcagaa acattgctga tccattctga gatcgcacct tttttcttgc
    38941 cagctattga aaaagaactg gtagaacacg gggtctcttt acgtgcggat gctcgtgcgt
    39001 tagaatatct agagacggct gctttagcag aagacacaga ttgggatact gaatatctag
    39061 attatatcct tgctgtcaaa gtggtcgatt ctttggatga agcaattgca catatcaatc
    39121 ggcataatac taaacattca gaaacgatcg tgacagatag ttatgcggct agtcaacgtt
    39181 tcttaaatga agtggacgcg gcggctgtct atgtcaatgc ctccactcgt tttacagacg
    39241 gatcggtatt tggttttggt gcagagattg gtatctctac tcaaaagttg catgcccgtg
    39301 gtccaatggg gttaaatgaa ttaacttcca caaaatatat catttatggc gatggacaaa
    39361 tccgcgaata atgtttcgtg tgaaacatta ttcccgcctt ttgccttttt tctgtaaagg
    39421 ttctatccaa ctagaaaggt gtcaatatgt ttgaaatttt aatgattttt tttatttacc
    39481 ttatttctct gaatatcgct gcttttctgg gagtcggtat tttatccctt ttctttcaat
    39541 tcaaaaaacg aagcataggg agtcaacggg aaaaatggtc gcagtatttt gacaaaatcg
    39601 ggcaaaaagg gctggttaca cgcttgcaca ttagttacat ggttgcatta tgcttattag
    39661 cgacaatcaa ttactattct tttttcgatc attcaatcgc ttatacgatt acgctactga
    39721 ttgctgggat ttttcactta tcttataaat atcagctgaa taaaaaccac ttgaatcgga
    39781 ctttccgcta aaaaatatcc gtacgataag tagagtgcct tgaagatcgg caaaaaactg
    39841 ccggactttt tagaggctgt gacaaagatt ttgtcacagc ctctttttcc atataaacgg
    39901 cgttcgccta ccagctgcct ggtaaatctg tttcctcttc gttaagaagc gaaaatccgt
    39961 caaatgtttc gccatcataa taacgaagca gctcatccat ggacaagttc atcgtttttt
    40021 ctttttcttc ttccgctgat tcagccagta gctgttcccg atacacctga tacataccag
    40081 ctattttttg ctgcatcgct tttctagttt caccttccgc attgattatt ggcaattctt
    40141 tgatatagcc acgataaatc aaccgatcat ctgaaaattc aatgggtaat tcaacgagtg
    40201 tcaacccgac ttcctgaaaa tattcttcca gcatctatta tccccccatt ctttttctta
    40261 aatgaatttt agcttaccta ctgaaaattt tccagtttca ttattttgag aaaaaaaggg
    40321 aatgtgacaa aaatcgtgtc acatcccctt tttccgaata aatggtgttc ttctgccaac
    40381 tcctcaatca taagtcacaa tattcaacaa acatgagaaa ctgttttttg aaattgttcc
    40441 ttgtgattca gagttaaacg acagtctcac aacctcttgg taaacggcgt tcaaaagctt
    40501 gcatctgcgg taataagccc aaccaaacga aaaatatgag aagctatttt tgtttggctg
    40561 ttcttatgac ttgatgcagg acagcttttt cacaacctct gatttcttat tcttcttcat
    40621 ttgtagcaaa ataattacgg tgataaatga tacaacatac actgataaga acaatggcac
    40681 ctgcaaaata aaacgcatag ctttcaccaa tatgttcacc aatcacacct gcaataaatg
    40741 gtgctaaagc tccaccgaaa aagcggacaa aactatagct ggacgaagca acgtttcgtt
    40801 gcagacctgg tatttccata gcaatcgtcg tcaacagtgt attgataatt ccttggaaaa
    40861 atcctgctaa cacaatgctg atcggtacaa ggactggcat agaagttcca gctcctaata
    40921 acagcaaaca aataatgaat aagacaaatg ctgctaaaat tgtccgataa gtcgtaaact
    40981 tccgctcaag agccggtgca acgaaaatcg aagcaatagc cagaagcaca ccccagccaa
    41041 aaaatacaaa accgacttct aattcagaat agccaatcaa taaaaatggc gcatacgcaa
    41101 gtaaagtaaa gaatccgaaa ttatataaca atgcgattag tccaactgaa cgaagtttat
    41161 gattgcttaa cgctttgaca ccttcccaaa ggcctacttt tctctttggt ttctcagttt
    41221 caggtaaaag taccgctagg gctataaaac cgcaaaacat caaaaatgct accccaaaaa
    41281 atggataacg ccatgaacca ctgcccaaga ccccaccgac tagagggcca acagccattc
    41341 cgcagccaag tgctgcttca tacatcatga ttgctttttc tgtgttacca actaaaatac
    41401 tgacgatcgt tgctaaagca gtagagacaa ataaagcatt cccgacaccc cagccggcac
    41461 gtaacccaat cagcattccg atattcccag ataatccgcc aagaccagcg aatacaacga
    41521 tgataaacaa tcctgtcatc aatgtttttt caaaccgatc cgactagata agaaacctgt
    41581 aaatagcatc accacaccag tgactaacat gtaactagta aataacaggg tcgtttcagc
    41641 tggcgtagcg tttaactgtg cagcaatcgt ttttaagatt gggtctacta gacccactcc
    41701 cataaaagcg atcatacagg tagctgctac aactagcgct gaacttttcc cctttgtttc
    41761 ttcatttgtt tgttcttgta tatctatttg attctccaat atttccatcc ctttctctct
    41821 attagcatca tacatgattt attttacatg taaatatgca catgtaaaac gtggaaactt
    41881 tactgtgata taataaagaa aataagacga gaggtaggat ttttatgaat actttagcct
    41941 atatattatt atgcgtgctt ttatccaaac cacgttctgg gtatgaattg aagcagctga
    42001 tcacaatttt ttgggaagcc catcacagtc agatctatac aacgcttgcc aagttagacc
    42061 agcagggtta tgtgaccgtt ttggacaaag acgagcactc tcaaaaaaag atctaccact
    42121 tgactgatga aggacgtgct cttgtagagg aatggatcaa agaggaaact cccgctccta
    42181 cgcaaaaaga tgaatttcta gcaaaaatct atgctttttc gacgctcgat aaaacaacag
    42241 ctactggact gctattcact agagaacaac agctgaacaa aatcatgcaa aaaaatcaaa
    42301 aaaaattgaa tgacctctcg ctagacaaga aacaagaatt tggacgttat gttgtgatcc
    42361 agcgcagaat tatgctttgt cagcaggaac tccaatggtg tcagcaagta agagaacaaa
    42421 tgcagcagtt ttttgagtaa gggataaaca gaacctttct agaaatatct attgaagagc
    42481 aaattatctt caataaaaga gcaatacagt tggaaaagaa aaagcgatcc gaacgacagg
    42541 ccaaaaccta tcgttcggat cactattttt ctagaactgc attcaagaga ctatgacaaa
    42601 aagcatgcca aggccttttt tcaaataaca gtgttccatc attcattact ttgaattgct
    42661 tttagcacac ttcttgaaag cgggccaacg atgatcaact gcaacggtaa agcggcaatc
    42721 acattcattt tccaagcatt catataagca gagaataatt gatcaggtat tccttgttcc
    42781 atgatcacac cgaataaaga catacatgtc accattccta aaaccatcaa gcatgaaata
    42841 gttaagatca tttggacctt gctttctgga tttactggta gtttaaaggc aatttttttc
    42901 gccagcacac cgatgatgaa gacatccaaa atgaaagcca ccacgaatcc aggcactaga
    42961 ccaatcaaca aacttgataa atgaagatct tgatgcagcc ataaattata cgcgctcatc
    43021 cccaagacca tcaaaaaaca catcattgtt gtaaaaatta ttccttcttt tttatttgta
    43081 ggcaatctgt accactcctt taaatttgtc acgaaaatca tcgtagcaca tttaaaaatt
    43141 tctcgtaagt acttttttta ttttctgccc ttttatttca taaacttcat cagccgtacg
    43201 ctcgacaaag taagaatcat gagaagtaaa caaaatcgtt cccggatata tttgcattag
    43261 cttctctaaa gcttcgatcg ttgccatatc aataaaatta gtcggttcat ctaacagcaa
    43321 cacattactt ggcttagtaa aaagcaaagc aatcgtcaga cgggtcgctt ctcccccact
    43381 taatgtacat aacggtttcg tcaaagtttc ctgagcaaac cctaagtgat tcagcaaact
    43441 acggacaagc gattctgaaa actctgtttg tctcatgaca aaacgaatga tggattcttc
    43501 agatgtcatt tgataaccct tttgctgata tacttgaaag cttactttcg gagagagaag
    43561 gattccttct ccttgtttgc ggatatgttc taataaggtt gtcttacctg aaccattttc
    43621 accgacaatc gcgatttttt tacctaaaga aaactgaaaa tcaccattta ccagtaatgt
    43681 tctcccactt ctttccaatt ggacattttg tcccatgatg ggatagcggc tgtggagttc
    43741 aagagatttt ggtactggaa aacggatttg tttcatctgc tgcggcttgg ttgtttctcc
    43801 tattttttgg agtcttctct ctaatgtttt cgcattcttt tggatggctt tttgtacact
    43861 gtctttttgt ttagaggaag acaaccgatc cggtctgatt tgttgctttt tctttttact
    43921 ggacactttt tccaactttt ccgcttgttt tttcttttct tggatcgatt tttccaatcg
    43981 ttttttctct ttcatgatcg actctgcttc tcgtagctga gtctttttct ccaattcctt
    44041 ttgagtgaga taggcactat aattccccga aaactctcgg atttctccgt cttttacttc
    44101 ccagattttc gatgctagct catctagaaa atagcgatca tgactaacaa aaaccagcgt
    44161 accataataa taacgaagct cttcaatcag tcgatcaata ctttttctat ctagatgggt
    44221 tgttggctcg tctagaagca tcccttgttc ataagaagaa agaagacgta tcaactcgat
    44281 ctttcgttct tcccctccgc tccatttccg ctctgaagtt tcttccagtc ttaactggaa
    44341 atgactggct aattccaaat ttaggtcctt tgcttcagcg agataggtta attgaggcag
    44401 gtagttgaag gtgatctctg tttggatttt cccatgatcg ggaaaaagtt caccggctat
    44461 tagtttgagc aaagtcgatt taccgacacc attttctcct atgatcccta tacgatcttg
    44521 ctgatagaca cgaagagaat caatagatag ttctagctgg ttgccaaagc tgacttgtag
    44581 atttgttata tttactgcta aattttccat atgttttaca ctcctatttc atgtaaaaca
    44641 acaattatcg atactaaaaa agagcagaaa aatctgtttg caaaaaaact gcatcacatc
    44701 ttttcctact ctgaacgaaa taactatgac taaatcacca aatctgtcat agctttcacc
    44761 tattctactg tcgttcgaaa aaatggacat actccatcca tgaaattgtt gtttatacat
    44821 gtcttttgaa tttatttagt tgttattcaa aagttcgaaa cgtaatttca tcgatgcagt
    44881 catttttccg acctcctttt ttattaggtt accacacacg attcaaaaaa agcaatagct
    44941 actgaatagt aactgaaaac cttttattcg aacgtagtga tcttttctgg tgctaaacga
    45001 acagactcgt aaccttcttc taccgctttt cctattgata aaataataac tggaacataa
    45061 cggtctttat ccaaaccaaa tgcctctgcc aactgatctg cttcgaatcc accaattgga
    45121 tttgtttcat agccatgatc acgcgcaact aacatgaatt gcatagccgc taagctagaa
    45181 tcgactttga ctacatcgtt catttcttct cttgaaaaac tcttgtaata aggaatgatc
    45241 gcagctaatt gttgatctct gacttctttt ggcatcttcc ctgattcgta agcttggtta
    45301 tagatttctt cgccatattc gtaacattgc atatcgccaa aaatcatgac cattgcagaa
    45361 gaagtatcat tttgtctcgt attgaaacgg atcaaaggac gcaagatttc ttttgcttcc
    45421 tcgctttcag cgaccacaaa gcgccatggt tgcatattga cagaagaagg tgcagtcgtc
    45481 gcttctttga tcatttccag catttcttcg tgtgggattt tgtatgtttc atcatatacg
    45541 cgaacggatc ttctaccagt cataattgtt gaaaaatcat ttttttgttc cattattatt
    45601 aacctctttc gtttctgttg ttatattgca aagttgggta ttcaatttat ctaaaagtgt
    45661 tagaaattgt ttcatttcta catcgctgaa agccggggat agcacggaac ctgtatcttt
    45721 atggtccatt tcacaatgct cgattttctt ctgggctttt tctgttagcg ctacatacac
    45781 ttcccggttg ttctgaggat ttctctgacg cgaaacatag ccatttgctt ccaatacctt
    45841 cagatgacga gtaactgctc cttgatcaat ctgcaacttt tcttgcaaac tgctttgtag
    45901 caatggattt tcttctttca gtctcatcat gatctcgtat ctagtaagac ttataccgat
    45961 attttcttca aagatctgga cgattttttg atcggctaat ttgatttgat ataaaaggtg
    46021 actaatctct tctaaagaca taatttcctc ctttctaata attgactcgt caacgattga
    46081 cttgtcaata atagaataaa aaaagaaaaa agtcaaagaa tcagctgtgt aataaaaaaa
    46141 acgctctcaa tagaaaagta tagagaaaag aaattttttg tagaatgaac ggaaaaaatg
    46201 ataaagaaag cacaacgcta aaaccaagat tattgattct tatcaaacta tagaagatag
    46261 ctcttctgca gtagaaaatt ttacaccgcc aaaaatttcc gaaatcgcct tttgcaagaa
    46321 tcccgtcgaa tattagaaat tggatcagta tgcttaaaca gtaataaaaa agttatgaca
    46381 agatgttgtc acaacctttt ttgagtaaac ggtgttcaaa aacttgcatc tgcggtaata
    46441 agttcaacca aacaaaaaac atgaaaatct gtttttgttt ggctgttctt actacttgat
    46501 gcagggcagc tttttcacta cctcttttta ataaccataa aatcctattc cttcatatct
    46561 ccagcccatc ggtgttagcc aatctctttc cttagcactc aaggtatagt gatgggtacc
    46621 agttttttca tttggattaa atagtcgata aattggcttg gaaccaccag aataaaaact
    46681 tagaccttca taacgccaac cgatttttgt taaagagttg atctcagatt gtgccattgt
    46741 gtaatgatgg tctccactgt aaggattgta taaacggtag acaggttgtc cgctgtttgg
    46801 tgcttgccaa gcaatccctt catattccca gcctacagtt ggcagataat ttttttctcc
    46861 ggcattcaat gtatataaat gttctcctgt atgacggtta tacagacgat acatcggaga
    46921 agttgaagtt ggtgctgtat catactgtgt cagattatag gtttcgatta cattgttcaa
    46981 tgtagaacca taattaggcg cagtggcata acgacctgtt agccaagccg tagcatcttt
    47041 gtaagagttc gtattgcttt tccatgcacc ggaatagtag taaacgcctg gctgaaaaga
    47101 agtcgtcttc aataccgtta cgttatcttg caatgattct ttgtacgaag gatattttct
    47161 aaaagcggca ttcaccgtaa tccattgccc attcacatat tccgaagtag gcatcgtaac
    47221 agattggcca ttatagcttc ctttaatccc aaatagattg taattagggg cagaagccaa
    47281 agtactgttc ccccagccac tttcaacgat ggcttgagca atcatgactg atgcatataa
    47341 gtcattggca cttgccaaac tttgtgcagt tgaggcaata ctatcaatga acgcttgttg
    47401 agaattagca gatgagcgag cataaaaagc atagccagta tagttttcga attcagacaa
    47461 atctgcttga tgggtatgtg tcaatgtttg cccatctgtc aattggttcg tgatttcttc
    47521 tgcattctct ggattgttct gagcttctgc tgatgtactg tcttcggttt gtgtttgttc
    47581 tgttgtttca gttgttggtt cttcggttga aattgtattt tctgagacag tagctgactg
    47641 caaagtactc tctgtatcgt tttcaattcc tgtttctgaa gaactcgatg tctgagttgc
    47701 gacagtttct gtcgattctt cagctagcac tgttgtgttg gcaactgcta gatttcctaa
    47761 aagaataacg ctaaataata aattctttgc tttcatcttt tttctccttt gaattatgaa
    47821 acttttccta aataacagtg taactaaaaa agtaaaagca agtcaatgtc catatttaaa
    47881 taacatttgt ttctttttga taaatttgct ttttctacat aaaaagacgt aaatgaagaa
    47941 tattcttctc cattcacgcc ttaaattgat ggtcgtttga ataactattt aacaagtaat
    48001 tctctttgga tattatccgt tatggattat acttcctgtg tcgtcggacg gataatcatg
    48061 ttactgacag acatccggtc tggcgtttct atcgcaaatg ctacagcaga agctatatct
    48121 tcggaggtca atccccattc tttttgcgct tcatgaagtt caagagcagc ctttttgtcg
    48181 ctgatggttt gatataattc agtttgaaca gctcctggag agatgatcgt agatttgatg
    48241 ttattttgcc gttgctcttg acgtaatcct tccatgattg cacggacagc aaattttgtt
    48301 ccgcaataaa cggcggaatc aggataaaca acatgtcccg ccacagaatc agttgaaatg
    48361 atatgcccag atttttgttt ttccatgatt ggaagtacgg cggaaattcc atttaacaca
    48421 cccatgatgt tgatatcaag catattttgc cattcatcac ggtgaccttc aactaatgga
    48481 gcggttggca tgattcctgc attattgtac aatacatcga ttcgaccgaa cttttccatc
    48541 gctaaatcga taactgcttg gacttgtgca aaatctctta catctgctgt ttgaatatat
    48601 agctcagctt ctggtaaaga ttcttttatt gcttttaggc gatcttctcg gcgtgcagct
    48661 ataactagtt tagccccttt ttctgctaat agcttcgttg ttgcttcacc gattccgctt
    48721 gaagcgccca tgatgacaat cactttttct gataaatcat tcattctatt ctcctcctgt
    48781 tttgaaaaat ctcttgctta tactcactat ctcttatttc atcttactgt tcaaataatc
    48841 cgttcgacag gtttgacgat tttggcaggg attcctgcaa cgatggtatt gtctgggaca
    48901 tctttcgtaa cagttgcatt cgctgcaaca atggcattct cgccaatcgt tacgcctggc
    48961 agaatagttg ctcctgctcc gatccaagca tttttcttga tgtggattgg ctttactgac
    49021 aagcctcttc gttcttttgg atcggttaga tggttcaccg tgatgaggtt gacacgagga
    49081 ccaatcaata ctgaatcttc taaacagatg ccacctaagt caacgaacat cacttgacga
    49141 ttgataaaga catttttccc tatactaata tgtttgccga aatctgtata aaaaggcagc
    49201 agaacagtca ccgtatcatc tactttgtca ctaatgatat tttctagccg tttgcggata
    49261 tctgctggtt catggtaccc agtattcagc gctgctaaag gctcttcgtt ttcagcttgg
    49321 atctgatgga tcacttcaaa gagcggatcc gttactcgaa tctctttatt gacgatttgt
    49381 ttttcgatga ggcttgtcat tttgctccct ccttttatcc ttactttata acggacggct
    49441 tatcatgtct aatacttata aaacatactt aactattcct tttaactata cttagtggta
    49501 tgctttttta aaaacaggag ggaaaaaatg gatattcgta tactgacgta ttttttagca
    49561 gtagctagag aaaaaaatat cagccgagca gcaaaatctc tgcatatcac gcaacctact
    49621 ctctcaaaac aactgaaaga tttagaagaa gaacttggtg tgacactttt cactcggggc
    49681 tcccgagaga tacagttgac ggattctggt cgatatcttt attcaaaagg aaaagaaatc
    49741 ttgtcgttgg tagaaaaaac ggcgaacaat ttaacaaatg acgcaatcat ctctggagaa
    49801 atctacattg gcggaggcga aactaaagca atctctttca tttctgatag ccttcattcg
    49861 ttgatcaagg cgcatcctga tatccagttt catttatata gcgggaatgc agatgatatc
    49921 atagacaaac tggatagcgg attacttgat tttggactcg taatcgaacc gacaaacaaa
    49981 caaaaatacc agtcatttcg actgccagcc caagatcgtt ggggactttt gatacatcga
    50041 aatcatcctt tagcaaagca aaccgtcatc catccgcagg aactgaaaac tgttcctttg
    50101 atcatttccc ggcaaacgtc agtggacagt caactagctg aatggctagg tttcagcata
    50161 gatcagttat cgatcgtcgg aacatataac ttattgtaca atgcttcttt gatggtcgat
    50221 aacgggactt ttggtgcatt agctctagat gggatcatca atacttatgg gacggaaaaa
    50281 atattcatcc cactcgatcc gccattacaa gcttcactga atctcatttg gaaaaaagac
    50341 cagcctcttt ctcctgctgc tcgtaaattt ttagatgtcc taacggagaa atacagttaa
    50401 gtagagtttt tttccttcgc tttcagcttt ttaacaacga ataagataag caaaagatcc
    50461 aacgattaaa taacataagt ttcttaattt atttcttcct tttatttctg ataattccag
    50521 taaattacta taattataaa aaaagaattg acaaaacata actgtctata taaaattgat
    50581 tacgttaaca ataattcgtt tcatgtgact agatcatact aggcatgaaa aatctctgag
    50641 gaacatggtt tccctaggag gtttttcgtg tctatttttg tttggccgca atcaactggt
    50701 ctagcaaacg tgaaaaccaa atcttcaaaa agggtgcgcg catgaaaatc aaaaagatct
    50761 ttaatcaaaa tgcagtattg gtagatgacg gtggacagga aaaagtagct attggaaaag
    50821 ggattggatt tgacagaaaa agaaatgatt tgatttttga tcgtgatgtc gaacggatat
    50881 ttgtcatgga accagaaggt caaatcaagt tgcaaaattt acttaatcag attgatgaac
    50941 gctttttatt tgcagcagaa acgatcattg atcatgcgga aactgttttg atggaaaagt
    51001 tgaatgaaca tgtattgatc gctttgaccg accacattgc tttttctgcg gagaacatta
    51061 ataacggtat cgtcatccgc aacaaacttt taagagaaat cgaagtgctt tacagtgatg
    51121 aattcagtat tgctcaatgg gcagtagagt atctgacaaa agagcttggg attccatata
    51181 cttatgacga agcaggatat atcgcaatcc atattcacag cggacgaagc ggtcggaaca
    51241 ataaccatcg cagtatccgt gaagtgacga ttgtatctga catcatccat ttagtggaat
    51301 cagaattgga aacagatatc cattcagaac cattttcact gaattattca agactggtga
    51361 atcatttacg actgcttcta caacgaaccc atgcgcaaca atatgctgtt ttagacacgg
    51421 aaatcgtaga aatggtcaaa cgaaaatacc cagaaagcta taagatttct aaaaaaatcc
    51481 gtgtgttgct gaccaaagaa taccagctcg cgatcacgaa agaagaactg ggttacttgg
    51541 ccatccatat cgagcgttta agaagcgcaa tcgtacaaac aaatgttaac aatcatgagg
    51601 aggaaaaaaa ttgaaagcat atctacaacg aatgggacgt tcccttcaac ttccagttgc
    51661 ggtcttacct gctgctgctt tactagtagg gatcggaaac tggtgggcat cttatagtaa
    51721 cgacattgtc gctcattttt tacaagctgg cggtaacgca gttttaggtc aattaccttt
    51781 gctgtttgcc gttgggttag cactagggat gtcagtggac aaatcaggtg ctgctgcctt
    51841 ggcaggtttg gtcgcatttg aagttccagt aaatgtttta aaaactgatt cagtagctac
    51901 tttattgaat atcaaaccag aatcagttga tccatctttt gctcagatca gtaacgtatt
    51961 tattggtatt attgccggat tgattgcggc agctctttat aatcgtttcc acgaagcaaa
    52021 attaccgatg gctttatctt tctttagcgg caagcgtcta gtgccaatct tggctgcctt
    52081 tgtcatgttg ctgatcagtg cagtgctact gctcgtatgg cctccggttt actctgcttt
    52141 agtttctttt gctaaattca ttttgagttt aggctggggt ggtgcaggtc tttatggctt
    52201 cttcaaccgt ttactgatcc ctacaggatt gcatcaagca atgaactccg tgttctggtt
    52261 tgacgtagca ggaatcaatg atattggtaa atttttggct tctgaaggaa caaaaggcgt
    52321 aacaggaatg tatcaagctg gatttttccc aatcatgatg ttcgggcttc cagcaggagc
    52381 atatgctatt tacagaaatg cacgtccaga aaacaaagca aaaacagctt ccttgatgct
    52441 agcagcagct tttgcttcat tctttactgg tgtgacagaa ccacttgaat tttcatttat
    52501 gttcgttgct tggccattat atgtcttgca tgcaattttc actgggattt ctttagcagt
    52561 gagtgcattc ttccattgga cagcaggatt tgcctttagt gccggatttg tcgattactt
    52621 cctatctttg aaaaatccga ttgcgaatca gccattgatg ttgatcgtcc aaggattagt
    52681 aacagcagtg atttactact ttggctttaa ctttgctatc aaaaaattcc atctgatgac
    52741 tcctggccgc gaggaagcag atctttcaga tgacgatact gcaacgacta attcagacaa
    52801 taaatatgcg gcacaagcaa gcaaaatcta cgctgcatta ggcggagccg acaatgtaac
    52861 ttcgatcgat aactgtacta cacgccttcg tttgcaagtc aaagatacag gcctgatcga
    52921 tcaaaataaa atcaaagcca ctggcgtacc tggtatgaag atcatcgacg gaaaaaatgc
    52981 gcaagtcatc gtaggtacag aagtacaatt cgttgcagat gagatggcaa aactacatgg
    53041 tggtgctgca gcacgacctg cacaaacaaa caccgtggtt aaaacagaaa catttgccgg
    53101 tgaaacgatc gaacaagata tttacgctat cgcaaatggg aaactgattc caatcactga
    53161 agtttcagat gatgtattcg cggaaaaaat gatgggtgac ggctatgctg tattaccaga
    53221 aaatggagaa atcttctctc caatcgctgg tacgatcaca aacattttcc caacgaaaca
    53281 tgctgtcgga atccaaacag atgctggtat cgaagtcttg cttcatatgg gcatcaacac
    53341 tgttgactta aaaggcgaac cattcacttt atatgtagaa gaaggtcaaa aagtcgcaag
    53401 aggtcaattg attgctttag ttgatcttgc cgctattcaa tctgcaggca aaaacacgga
    53461 tatggtcgtt gtattcacaa atggcgacaa agtccaaagc ttagaaatcg aacccgcacg
    53521 tgatgtaaaa gcaaatgata aaatcggctc tgtttccaac aaagcctgag ccatcgtctt
    53581 ccccagactt ttttatcgat aaacaaacag gagctgagac aaaaatcttg tctcagctcc
    53641 tttttccgaa taaaaacggc gttctcctgc caactccgcg gtaataagtc cagctaaacg
    53701 aaacatatga agagctattt tttatttgac tcttcttatt acttgatgca gaacagcttt
    53761 ttcacaaaat ttttcttcat actcaaaact aatcggcttt ttcacaacct ctttatacat
    53821 tattcctgat ccaatatttc ttttaatgct gcttcaagag aagttaatgg ttttcccaat
    53881 actttttcta aatcatcaga ggaaatagct aactgatcct ttttaatatc atgttggatc
    53941 gctaaaaaca tgtcagctgc ttcttgaggg aatccggctt cttttagatt ttcgacaaac
    54001 gctgcatcac ttgcttcaat aatttctaac tctttacctg tcgcacgctc taatgccttg
    54061 gctaattctt catagtcagt tagattgccg gataattcta agatttctgg agagtcactt
    54121 tctactagta cttttgctgc agcttctgca tattcacgtt tcaatgccca acctgttttt
    54181 ccatttcctg cagcataaac gaattgacca gtttttaaag cctgcccaat gataggtaat
    54241 tcattctcta cataccaatt attccgtaaa aatgtatggg aaatacctga atccttaatc
    54301 attttttcag tgaattggtg atctggcgct aatacactga tcgagtgatc ggcatctgca
    54361 aaacttgtat aagcgatata agatacccca gctgcttgtg ctgccttcac tacattctta
    54421 tgttcggctt gacgatttcc tggaacgcca gagacaaaaa gcaatcgatc tattcctttc
    54481 aacgcttctt tcattgagtc taaatcatca taatcaccaa tacgtatatt aaatccagct
    54541 tctttcagtg cttctccttt ttcttcacta cgtactagta catagatttc attttgtgga
    54601 accatctttt ttaagaattc cagcgcataa ccaccaaaac ctccagttgc tcctgttact
    54661 aaataatgca tatttgccac tccctattct ttttatttgt taagatttac agctgtacat
    54721 ttataaatta cacctttatc ataaagagtg tggtaagata agtcaagcaa aaaaaatgaa
    54781 aggatgacaa ctgatgaagt attctctgca atttagtgat gcaatccatg tacttgccta
    54841 tattgaaatg tttcaagata cccgatcact ttctagtgag atgattgcca gtagtatcga
    54901 aacaaatgca gcaaatgtac gtaaaatcat gagtcagctc aaaaaatccg gtttgatctt
    54961 tacaaaaacc ggaaaaccgc agccttccct tgccaaaagt ccagatcaga tttctttact
    55021 agatgtctat cgcagcatcg aaggtaatac acagcttatc caagtcgatc caaaaacaaa
    55081 tccaaattgc gtcgttggtg cgaatatcca agaagcttta gaagaaagct acgctcggct
    55141 gcagaaaaag gcagaagaag aaatggctaa gatcacactt gatagtctga tacataaaat
    55201 agccgttttg gaaaaagaaa aaagaccaga aaacacaggc ctcgtagaga gatttttata
    55261 attctctcta ctgtgcctgc atttttctga tcggaatcgt ctacataaat tcttttattc
    55321 tccgggggac aaaagccttg tcatgcccct ttttcctctc taatgctcag gtcaaagcgc
    55381 tgaagtcatg acgtcttgat aaacggtgtt caagtagctg accctcggca ttagttctga
    55441 aaaatgcaaa atatggaaag ctatttccca tttttcctct ctaatgctca ggtcaaagcg
    55501 ctactttcac gacctctata ttatcatggt ttattgacta ccggtatcca gatttctccc
    55561 atatatttcc cgttggtaac tcccatttcg actacagcat ttggaccccc aacataggcg
    55621 taatcttcta cttctcttaa aacatgccca aaggccgtat tcgtcaaatc atttcttaat
    55681 tcttcaggcg attctgctaa agcgcttaca acaagatacg ttccttttgg gaatgggatg
    55741 attcgcgtag tgtttggaac atcctgactc gtttgaacac cgacatagta catcattttc
    55801 ccgttatagg gttcgttgac tgtaaagaaa taatgatttt cagcagcttg tttcaatgca
    55861 tctattgttc cattaccgat aactttttgc tcaaatgctt gtttctctgc agccaaaccg
    55921 acaaaatccg tattgctact cttcaattcc acaccaatac ccgtcaccgt aaaatctggt
    55981 ttttcttcga tcctatactt tgccatttct cccacccttt ctattatatt aagttcatct
    56041 tacataaaat taaagggaaa tgacacgatt acgctctttt ttctagggat atttttattt
    56101 tttaactatt tttttgcttt cactgcaatc caaatctcac tgtaaacatt actgaaatcc
    56161 tctccccatt ttgtaaaaga gatctcggga gcttcgacta acccataatc tgaagaagga
    56221 agccattctg catagatttt cccccaagta tcttgtaaag tttggggaaa tggcccttta
    56281 ttaggaaaaa tggcccaagt gtgttcttct atagcaagca tttctaaatc agcgtacgga
    56341 ttttcttttg tcgtagcaaa gccaatcatg tgcatcaaac tgcccttttc ttccaagcgg
    56401 tctccatcaa atccataaga aacgttgatt acttgatttg gatataaatc tcctaactta
    56461 tgcatctctt ccctttgagt ctccgtgatc gtctgcgcta acttttgtat ttcttgattg
    56521 actccttcaa atttgacagg aacttttttc gccacacctg ccacataaaa ctgttctttc
    56581 ttttcgatac gaaattccat cgattttcct cctctgacat caataaaaaa tgtcaagcgt
    56641 ggaaaagatt tttgtatgcc gtatctactg atttctgaag gcagatatcc tgaccaatca
    56701 cgaaaagctc ttgaaaaacc ttcgactgtt tcatatccat atctaaacgc cgtttctgtc
    56761 actcttgatc catctagaag atcgcgatta gccaaagcca atctacggtt tttgatatag
    56821 tccattagcg acatccctgc aataaacgta aagatgcgtt ttaattgata ttcagagatt
    56881 cctgtatttt cggctattat tttataggaa agtccttctg tcaaatgttc ttcgatgtaa
    56941 tcgatcaatt ggttcagatc tttaagcata tttatcttcc tccttgattg cctttttatc
    57001 atattatcaa agaaagtcaa ttacttgatg aatcaggaac caatttagtc agatgtagaa
    57061 aatgttgtat ggatctaaag aaaattctca agcagcgttt cgtaacggat aattggtatt
    57121 atctgcctat ctatgtttta cctttagaaa aataaatttt ggggaagaca gattctaagt
    57181 aaacaaagcg gtaaaaatat cttttattcc cttagttaat ccgcttacta cctactgtac
    57241 aaagcaaatt gttgttcaaa ctgctgatca gaacgctgct tggatttttc tttgcgatac
    57301 atcatacctc ttttatcaat tggaacaatt atttatttct aacaaaatca cttcattttt
    57361 gtcagataaa taaaaaacaa aaaaagttcc atctttaaaa cacgctattc ttttactttt
    57421 tctcacattc tctaagaaaa gaaaaggaga atgaattttt gacaattcga gcaaaagtga
    57481 ttatcaaata tgtaaaatca gctacttttt tcttaaacgt ttctttatac taaatataaa
    57541 taagtgtttc atgaaaggag aactagatga ataattggct tgagtatttt cctgaaaacg
    57601 ttttggaacg cggttacagc tatcatctgc acggttttgt ccgtcatttg aactatacaa
    57661 gcaagtactt gtccgcaaca gtttctggca cggaagatta taaggtagtg atcacttggg
    57721 atgaaaagac caacatgact tgtgattgtc tatatgccat agaaggaaaa aaatgcaaac
    57781 acatggctgc tgtcttattc gcttacgaag aacgtccaat aaaaaaatcc aactactcac
    57841 tttccgaact ctcgtccctt gtttcctccg ctagttcctc tcttgtacgg gagcttttaa
    57901 cagagattct tatagaacat ccgcacttta tcgaacgctt caaagttaag atgccatttc
    57961 atgcaataaa ttattcagat aaattaacaa ctatcatcca taaatacgat catattatca
    58021 agaaaaacaa aaacaggaaa acagcgaagt tcatcatgga gatgcgaaag ttcatccaag
    58081 aagcagttga atcgcttatt cagcaaaatg cctatcttcc cgcattcgaa ttgatcaatg
    58141 aagtgattgc tactctcgaa acattctatt gggagcctga ggatgaacga acccttcttc
    58201 tgatagagga ctgttattat ttatggaaag aattattagc agaagcacct catgcagaaa
    58261 aaaggcaaat gttctcttgg ttcgtctgcc aagtcgatca tactgacgcc tcttattcaa
    58321 aacgctactc tataaaaatc ctgaaagaag actttcggga aaaagaattt tctaaccaaa
    58381 agaaaaaaat agataaaaaa ctaaagaaac ggtaaagaaa gacgatttca atgaaaaata
    58441 gcaagtaaac gataacaacc atcctaaatc cacaagaaat acagcagacc ttgctttaaa
    58501 aacacgaatc atgcaaagga tgaagtaaag aagacttaac gcgtacccaa tattagtcat
    58561 ttgactatta ggtacgcgtt ttgttttata agtcatgaca aatttccttc gttgctggat
    58621 agattttccg gcattgttga tagattttat gatacttttc tacattttgt aagagaggtt
    58681 ccgctgcatc tgtatattgg ataaaattct gacaacaact ttcaggtgtg tcgaaccacc
    58741 ctaaccctag tgcagcaatc attgttgcgc ctaatgcagg tccttcctcc atacttaaac
    58801 aatagaccgt tgcatcgaaa atatcagctt ggatttgtag ccatgcaaga tttttcgcgc
    58861 ctcctcccac cgaaacaata cgtgtaaatc tgcgacccgc tagttcttta cgtttcaatc
    58921 ccaccattga caaatgctca ttaaaaattt agaatattca caagtttaac gacaattatg
    58981 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
    59041 cgatcaaaga cacgttacct accgttttcg gttatatcgg tattggactt gcatttggta
    59101 tcgttgggaa agctgccgga tttcatccac tagtcgtcac gttgatgtcc ctgctggtct
    59161 atgctggttc tgcccaattt atcacagtca gcatgcttgc tagtcacagc ccattgcttt
    59221 ccatcgtttt ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
    59281 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
    59341 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
    59401 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
    59461 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
    59521 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
    59581 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
    59641 tcccaagcaa cttagtgatt gtagttgtaa cactgattgg gtgcggattg ggggtgtgga
    59701 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
    59761 tggctttcta gggtcctacc atttgtctta ctgaaaaaat tcgatttacc gcaaccgttg
    59821 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
    59881 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
    59941 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggcaggaatt
    60001 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
    60061 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
    60121 atccgttgaa cggaccacta acaacgactt ttcctaacat atgtcctgtt tctacgtctt
    60181 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
    60241 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
    60301 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
    60361 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
    60421 caaatggtcg gacaaggtgc ttgatttgat caaagtagcg ggtaatatcg aataatacag
    60481 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
    60541 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
    60601 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
    60661 cggatccgtt gatcactaag atttttttcc cgcgattggc attttcttct ggagttagac
    60721 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcagctt
    60781 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaact agttggtatt
    60841 cttgattgct tcctgctctt gttgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
    60901 atttttgcac ttcgtcacct acagcaacaa ctttcccgac tccatcaaaa cctaagatac
    60961 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
    61021 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
    61081 caataaagct ttttggatca tcgatcggca acccttcata aaaaccgact accttcatta
    61141 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
    61201 gttcatttca atatatatag tatgctattt gttccacctt ttttattcat cataattatt
    61261 ctcttactta aagtaaacta tttgcacttt tttggttaaa aaagaggttg cgacaagact
    61321 tttgtcacaa cctcttataa acggtgttct cctaccaact cttcggtcac aagtcaaaat
    61381 attcatcaaa catgagaagc tgttttttga aattgctcct tgtgactcag agttaaacgg
    61441 caggctcaca acctctgata taaacggtgt tcaatcaact gaccctcggt attagctcaa
    61501 aaaacgacaa aatatgaaga gctatttttg tttggctgtt cttattactt gatacaggac
    61561 agctttttca cgacctctaa ttcacggtgt tcaagcaaga aatcattgtt cactgaaaag
    61621 cgttggatgt tctttttcaa aggtctctaa gatctctcgt acttcatcag tagatttcgt
    61681 attcatcaac tgaactcgca agtcatttgc tcctggaaat cctttgatat agattttaaa
    61741 gaaacgatgt agtcctgtga tggaacgcgg gatgatttct gaataatgat cttgcagatc
    61801 tagttgcaga cgaagtagtc cgatcagttc tttttctgta tgttccctcg ggtctttttc
    61861 aaatgcataa ggattcttga aaatccctcg gccaatcatg atgccatcaa cgccatattt
    61921 ctcggccaat tcttctcctg tctggcgatc ggggatatct ccattgatcg tgattagtgt
    61981 ttgtggagcg atttggtcac gtaaagcaat gattttagga atgacttccc aatgtgcatc
    62041 tactttactc atctctttac gcgtacgcaa atggacagaa agattggcta tatcttgatg
    62101 aagtaggtga ctgatccatt cttccatctc agacatttcc gtatagccga tacgcgtctt
    62161 gacgctgaca ggcaaaccgc cagctttagc tgcttcaatc aattgtgccg ctacttctgg
    62221 acgaaggatc aagccactgc cttttccacg ttctgctaca ttagggactg ggcagcccat
    62281 gttgatatcg attccttgga agcccatttc tgccatagca atgctcattt ctctaaaaaa
    62341 ttctggcttg tctccccaaa tatgggcaac catcggctgt tcatcttctg taaatgttaa
    62401 tcttcctcgt acactgtctt ttccatctgg atgacagaaa ctgtctgagt ttgtaaactc
    62461 agtaaagaag acatccggtg ctgctgcatg ctttacgaca tggcgaaata caacatctgt
    62521 tacatcttcc attggtgcta aaacaaaaaa aggctttggc agctctttcc aaaaattagt
    62581 agtcatttat ttgttactct ccattctatc aagcagtgaa gtcgcaatct ttctcaaaaa
    62641 cattgtcatt atactaggaa acaaaaaaag aagcaaagtg aacagcttaa aagatgtttc
    62701 tgattgagca aaaaaaagac tgtgacaaaa atcttatcac agtccatttt ccggaataaa
    62761 tggtgttcaa aagcttgcat ttgcggtaat aagtccaacc aaacgaaaaa taggaggagc
    62821 tatttttgtt tggctgttct tattacttga tgcagaacag ctttttcaca acctctccac
    62881 taaacggtgt tcaatcagct gaccctcggt attcgctcaa aaaacaacaa aatatgagga
    62941 actattttcc cgtttgtttc tctaatactc aggtcaaaac gctgatttca caacctcttt
    63001 tctaacttct caatcctctt cctttattga tcaaatacca agtaacggcg tacaaaacaa
    63061 cgatgaataa cacaagtacg atcatcgatg ttgctactgg cacatcgatc gttccaagga
    63121 atccgtaacg gaaaccggag atcatataaa cgatcggatt gacttttgaa acagcttgcc
    63181 aaaagggcgg taacatagag atggcataga aaacgccacc tagataagtt aacggctgca
    63241 agacaaatgt aggcacgatc gatacatcat cataagactg agcaaaaatc ccgttgagca
    63301 gtcctgctaa tgaaaacaag attgccgtca tcaataatgt aatgatgacc atgaaccatg
    63361 aatacacatg aagtggtaca aagaacaagg aaatgatcgt tacgagagag ccgacgagaa
    63421 tactgcgacc cacaccacca atcacaaagc cccaaataat cacatgcgta ggaacaggtg
    63481 ctaccaagat ttcttcgata tttttctgaa atttttgaga gaagaaggac gaagaaacat
    63541 tggcatacga actagtgata gcagacatca taatcaaacc aggaacgatg aattccatat
    63601 atgaaaaacc acccatgtca ccgatccgcc caccgatcat ttttccgaaa ataacaaaat
    63661 acaaggatgt cgtgatcact ggcggaacca gtgtttggac ccatatacgc aaatagcggt
    63721 tcgtttcctt gatagctaaa ctttttaatg cagtaaaata aagattaaac atttttctcc
    63781 tccgtatgat tattttcttc tgtgattttc aagaacagtt cttctaaacg attcgactta
    63841 ttcctcatag aaagaacttt gattccttgt cttgtaagct gatcgaagat tccgttcacc
    63901 ccttgatttc gttcaacttc gacagcgatt gtctgctcat cttcaaatgt acaagcatat
    63961 ccttcaatca caggtttggt atcatatggc gctaaatcaa agataaatgt ttcaaattgc
    64021 agttttgaca gaagctcttt catacttgta ttctcgatca gttcacccga ttggatgatt
    64081 ccgatgttgc gacaaagcat ttcagcttct tctagataat gcgtcgtcaa aatgatcgtt
    64141 gtaccatttt cgttcaattc tcgtagaaac tcccacatct cgcgtctcaa ctcgatgtca
    64201 actcctgctg ttggttcatc caagatcagc aaacgtggtt catgcatcaa tgccctagca
    64261 atcatcagtc ttcgcttcat tcccccggaa agcatacgtg cacggacatt tctcttttcc
    64321 caaagattgg actgcttcaa atatttttcg ctgcgtttca tcgcttcttt gcgagagaca
    64381 ccgtagtagc ctgcttgatt cacgacgatt tgctggaccg tttcaaaagg gttaaaattg
    64441 aactcttgtg gaaccaatcc aatctgttgc tttgctcgaa ctagatcctt gtccagatca
    64501 taaccgaaga cattcacttt tccagatgtt ttattgacta aagaagtaat aatcccgatc
    64561 gttgtagact tcccagctcc atttggccct aatagcgcat agaaatctcc ttctttcacc
    64621 gaaagatcga ttccccgcaa tgcttcgacc ccacttccat agatcttttt caaattattt
    64681 atttcaagcg catttgccat ttatttccta tctccttata gacatgattc ttaattgtat
    64741 catggaatac agcagatgaa aaaacaactt ggcttccaca caaaaacaca cctgctaaca
    64801 aagaggagat gtgttttcta gttcatgatc atcttcgata gaataactat ctaaacaact
    64861 ctggaacttt taaaatcata aaagtaccga cagcaactac tacgatgaca tatagaagat
    64921 ccttataact gaggttcttg tcttttttat tcatgttaag ctttcctccg tccttttatc
    64981 gaaagctttt ttcttttatt ttaaccgaaa taaataaaat ggaaatagtt attttcaatt
    65041 tctgttttcg ctgtttgttt tacggagaac tttgccatgg aacgtgcgtc tcgtcgctga
    65101 tgctaagttt tttcgataaa agctgctaga aggaagacag cgtggtttat tttgtatcgt
    65161 cttttgctaa atcttccaag atcatctgga cagcagactc gttcccattg aattctgcca
    65221 cttttttggc atcaatgtac ttcttttgat aatcgtcttt ttggacccac gcatcatgct
    65281 tcgtttgata aagttaatca aacggatacc actgacgctg ttcatcgaac aacaaacttt
    65341 ctttttgggc ctgttcaact atggatttag gataattcaa aactttcagc aaggcaatcg
    65401 catttcgggt ccttgaaggt ccttctttta atttgaaatc aaaactgatt ccttttcctt
    65461 ctgttacttg ttcttcaaag tggacattct cacaataatt ctttaaaatc tctgtcaatt
    65521 cgatatcatg tgtggcgaca aatgctaaag atggatactc cgcaagccac cgaacgactg
    65581 aagcggaaga agcaatccgt tcgatcgtat ttgttccttt taaaatctca tctacgaagc
    65641 agtagcagcg ttctttggaa gccacttggt ccagcaaacg tttgatcgat ttgatttctg
    65701 aaacaaaata actgtcgcct tcaaataaat catcttcgat tgccatggaa gtcaacacat
    65761 gtccaggttg cagggtaaac tgttctgcaa tcgctgtttg gatcgtctgt gctagaatac
    65821 agctgatggc tatacttttg acatacgtag attttcctga tgcattggaa cccgtgacta
    65881 atgtattctt ctgccagttg actggattaa ctaccgcttc ttttaacaat ggatgataga
    65941 tatctttcgc tgtcacaccg ccctcagtaa actctggtcg gcaagtgatc ggcatatagg
    66001 tgcgataatt caaaacggct gctgcaactt ccaattctcc cagcaattcc cacagcttga
    66061 tggcttcagt tgaatgcttt tctaatttag tgagaacgaa atgataggag ataaatggca
    66121 gcatgaacat gatgttgaca tattcaaaaa tcaattctgc ttcactcccg ctcttcattc
    66181 gaaatgaaaa acctaattta gggatgtctc ttaatggttt agtttgctgt ttcagctctt
    66241 tttgaagtgg tgtcgtcatt tttgctaact gattggcggc tgaaatcgtt gaaaccaaat
    66301 aactcatgct attcagctct gtttctagtg tttgtttctt cactagataa tagaccgtat
    66361 tgaatactaa gccaccgatt gctagataga ttcctagtac ttgccctaaa agaagcaaac
    66421 ctacacccac cagcggaagc aagccaagaa aaataaataa acctacgcgt ccaatctttt
    66481 tactttttcc atccgctaga tattgttttg caaaattatg atcttttttt cctaaacgag
    66541 cgaattgata ttggattctt tctctcagtt gaggattttc ctgataaaaa gcaattagtt
    66601 tttctaactg ataatcttct ccaaaatcaa agctacgtaa acgctgatac aacgcttctg
    66661 aacctacact agaataggta ctgttgatcc cttcaaatac ttcgaacata tccaagtcat
    66721 accaagtaag atcgtcgatt tcactatccc atgaacggaa ttttttctcc gtgagccacg
    66781 catccttcaa actttcttct ttatcaaatc tcggttgata aggaattttt ccccaattac
    66841 tacgaactgt ttcctttact ttcaaacgtg aaaaaatgtc taaaacaact aataaaataa
    66901 caactgcaat aataattaaa acgataaatt ctctttggcc catatccctc tccgttcctt
    66961 gatttgttag ttttagtata gcactgttgc gctatttgaa aagggggtac aaaaataact
    67021 gtcttcgtta cctaattcgt atcaatcaca ccaaaataaa aacaccttcc aaaaaagaag
    67081 atgtctttaa aacgagatac aattttttgt cattcacttc aggtaaatta gagccaatta
    67141 aaccctccgc aacttgacgg agatggagaa gatgcccccg tctaatctag cggtcatctc
    67201 tcctcccatt ccttccacca atttttttga caggtaaagc ccgattccgc tagattccgt
    67261 atttgtgcgg ctggtatttt cggtataaaa acgctggaag actttttcaa cagaggtttg
    67321 cgctcctggt ttgataggat tggccactgt taatcgaatg aatgaatcct cttttgaaaa
    67381 tgtcaccttt gcttgtccat ctgcataacg caataaattc tgtatgatat tttgcagcac
    67441 tcgcagcaac atgagcggat ctgctaatac agttgtattt atcgagtgtt cagcgaacgc
    67501 tacctcgata tttttctcag aaaattgttc ataaaaagag aaaaatactt cttccacctc
    67561 tttagaaaaa gaaagcgtat ggagatccat ttctttttgc ttcgtctcta ttcgagcaag
    67621 atcataaaac tgatccgtca tactgagtaa tcttgcaaca gattgtttga tgatcgttaa
    67681 atacttctcc cgtttctccg tatcctctac ctgttccagt aattgtacat aaccgttaat
    67741 agcagtcaaa ggagttctga gatcatgaga aaggccgacg atcgatagtc tgatgttttc
    67801 ttccatttct tgaaatgctt gtcctttttg ttcattttca tccacccatg tattcagtgc
    67861 atctactagg tccattagat tcttttctcg aaattcgact gaaagacgac caccatattc
    67921 ttgtcgttca gaaagagtgg cgattttctt agttaatctc ttgatttccc tttgcaggaa
    67981 gaaaataaaa cttcccataa agataacaac gagaagcaac agccacttaa tcaaatctaa
    68041 accccactcc ccacaccgta tgaatataca cagcatgtgc ctttgctagt ttccctcgaa
    68101 gattggaaat atgcatattc actgttttct cactatcaaa atatggttct tgccaaactg
    68161 tttcataaag atttgctcgc gaaaatactt ttttcggatg ttctaaaaaa agcttcagca
    68221 gatcgaattc tcttcctgtc aaatggacag ccacaccatt cacatttaca tccctcgact
    68281 ctttgtccag taagatttcc ttgtaataga tgcgttgcga cagatctttt acttggacag
    68341 aagtctgatg acgaagttgg atgccgattc gtaacaacag ttcttcgata tcaaatggtt
    68401 tcgtcacata gtcatccgct cctgctgcca acaaggaaac tttatctctt tgatcgcttt
    68461 ttgctgaaag aacgatgatc ggaacattcg atgtctgtcg gatttctgct aacaatgctt
    68521 cacctgaaag ccctggaagc atgagatcta gaaggatcaa atcgacagga tatgtgctta
    68581 acaataattt tccttccgta cctgaataag catcaagaag tttgtatctc tgttcaagaa
    68641 cctcctccag taaagaatga acagcttcat cgtcttcgat aatcaaaata gtttccagca
    68701 ggaacaccta cttaatgtca gatttttgga aaataaacat tcctagagct gatgatacca
    68761 cgattgttac tagactgacc agaatcattt tcggtgcttg cgaataagtt gcttgttgcg
    68821 caaagtccgt atacatgagc cacgtttccg taaaccactc cgttagaaat tccaagcctt
    68881 tgatttttgc gaaaacaccg ggaacgaagg ccgttcccat gatataaaga aaagaaagaa
    68941 gcaaactgac actggtactt ctagtgatga atgtcaaagc cactggaaca gataatgtcg
    69001 ctaatatcag cagaagcaat acaggaaata cttttagaac ataatttcca tagtttttga
    69061 ttccctcaac ttcaccaaat ccgaaagcaa gtgtgtaagc aatcatggct agccccgtaa
    69121 tcaaaaccac gccaagtgtt gtgatcaaaa gcgtaacgag aagtttcgac acataataag
    69181 tcattcgatt ccgtcctaaa gataacgtat tgcgaatcgt accgttttga aattcttccc
    69241 caatataaaa gcacgcaaat gctgcgaaag ccagaagaat ccatgcagcc attttttcca
    69301 ccattaaaat ccctgttgca ccgttgatga ctgtatccgt tgcttggttt aattcattgc
    69361 cgtattttgt atgcatatac cattcgttcc aagaataaaa aagcggaagt acgaccatca
    69421 gtcctagtga gacccacatt ttacggctct tccatacttt tattttttca gcagttaata
    69481 gattaaacat gtggattgcc tccagttaat tgtaagaaat aatcttccag tttttgccca
    69541 actaggtgga taccatccac aggaactttt gcctctacca atgcttgatt gatggctgct
    69601 acttggtcaa ttccttcaaa aagctggata acacggctag attggacaaa ataatcccta
    69661 taaccaagag agtcgagaac cgttactgct tgttccgtcg ccgaagtgtc aatttttata
    69721 aattgacgag attcttgtgc caattcttct ttcgataatt ctttgatcaa gtgtccttca
    69781 tgcaaaatac cgtagtgagt ggcgatctgt gacaattcat ctagcaagtg gctagaaagc
    69841 aagacggtga tgccccgttc ttttaccaaa cgttggatga tttctcgcat ttctgccatc
    69901 cctgctggat ccatcccatt cgtcggttca tccaaaatca aaaattctgg attcgtgatc
    69961 agtgcatttg caatagctaa acgctggcgc atacctaaag agaaattctt tgctttcttt
    70021 ttccctgtat tttccaatcg catcattttc agcaggtctt cgatttctct atccgacaca
    70081 ccgccgttcg ctgcttggat acgtaggtta tctcttgcag ttaattctgg ataaagagca
    70141 ggggtttcaa tcgattgacc catacgtcgt cttgctgcct gcaaatccgt cgttccaaat
    70201 aattcgatcg aaccttcatc aatcgagatc aaacccataa tagttctcat aaaggtagac
    70261 ttacccgctc cattctcccc aatcaaacca tagatcattc cacgtttgat ttcgatcgat
    70321 actttatcta atgctttggc agcaccgtat tttttggtta taccagttgc tttcaagacc
    70381 gtttcatttc caaaatccat acttatcctt ctttcttatt gataagtgaa gtatagttga
    70441 cgggtataaa gaaagcagcc agaaatgtat aaagaaagtg taaattttcc aattgaattt
    70501 caagaaaaat cagactgtct cgcagacgtg gttgagttgt ctgccaatca aagagcgcac
    70561 caaacagtgc taatccaagt ggactggcca ccatggtaat cgttgttatc gttgacatca
    70621 gacgtccttg tatatgcgtt ggaacttctc gctgaagcca gaccatataa aaagcaaaag
    70681 aaaacattaa tccttaaaaa cggacttatg aaaaataata catgccgttt gggatttaca
    70741 attagctacc aaataagagg ttatgacaaa agacttttca caatcccttc ttccgaataa
    70801 acggtgttct cctgccaact cctcggtcac aagtcacaat atttatcaaa catgagaagc
    70861 aatttttgta aattccttct tatttcttga agctgaccac ttctgtcaca acctcagaag
    70921 cggataaata atattgtttg atgatacttt atctttttcc cgtttgttta tagatcgaat
    70981 gatattatag accaagaatc ctacctatta acatagctaa aaacacaaaa aaggcacttt
    71041 ccaaattgtg gaaagtgcca gaaaagttag gattaaggta actgcgctca gcattgcttc
    71101 gcccagtacg attctcgtaa ctcgtgaact cgaacgataa tcgcaattaa cgttttgaga
    71161 actgtgaagc tttacgggct ttcttaagac ctggttaaac aaaactctat tttattgcat
    71221 tttacaggaa tgtatttcat gcgaaattaa taagtctttg tttatagagt tttactaggg
    71281 tataaaaagt tgtatgttat cattacctca aaacattacc tcagcttggc gagttggttg
    71341 attgaagaat aaatcattta tctagatatc tcaataaaaa atgatttcta agaaacttgt
    71401 attttcgtgt tattattttt ttgccctatc aaaatttcta attcttctag cgtaatatga
    71461 ttatgacaca taatcgattc aattacttca atacctattt ttttcgagaa agatcccttt
    71521 tgttcactca caaaaatact ttcagaagac atatataagg aacgaagttt tggaattagt
    71581 atatcgtaca aatagaccac cataaaatcg tccttgcaac cattctcaaa aagaattaaa
    71641 tttgctgatt gtgttacata tatcactgaa ttacattcat ggtcaattcc aaaaacaatg
    71701 tctacttcat tttcttttct aattcgcgca gttacatttt tcaaactttc accattactt
    71761 ataaatgctt tagttactgt aagctctgat gtttgttcag atattccttt tataatattt
    71821 tgttcatcag ccacattccc cataaatccg ccgattcctt ttaagtaata attctcacct
    71881 aagagtcgct tgcattcact gaattgataa aaaaaccagt taaaaaaatc ttctggaaaa
    71941 ataaattctg atcgaacttt atttatattt ttagctccta ttaattttat aactcgatta
    72001 atgttcactt gctgcgatga acatataact gaataaattg agttgtttaa ttcaaaaaaa
    72061 caactttaac atatgcatca ttaattcgtt attttttagg tagatattga tcattgttat
    72121 ctctcttttt ttgcttccca tactccaact tagaaattgc cgttataaaa tttattttaa
    72181 aggtgacttt ttcccctgaa gtatcggtag taggaatact taatacctca gcttctgagg
    72241 aggtatccca attatagtta tactttgcat catcaccata aagaacaatt ccattttttt
    72301 tatcttgtgc cgttagcttt actggttcgc ttacttttct tgttaatatc ttggacttta
    72361 catcatcgat attgcttatt ttatcaagct taaataattt acaaattaat ttcgtactag
    72421 tttttatatt tg
//
LOCUS       DACECY010000007        62165 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.007, whole genome shotgun sequence.
ACCESSION   DACECY010000007 DACECY010000000
VERSION     DACECY010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.007"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(148..2301)
                     /locus_tag="IWO38_000480"
     CDS             complement(148..2301)
                     /locus_tag="IWO38_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ0312596.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVLVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2594..3442)
                     /locus_tag="IWO38_000481"
     CDS             complement(2594..3442)
                     /locus_tag="IWO38_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0312597.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3666..5606)
                     /gene="thrS"
                     /locus_tag="IWO38_000482"
     CDS             complement(3666..5606)
                     /gene="thrS"
                     /locus_tag="IWO38_000482"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ0312598.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5943..6122)
                     /locus_tag="IWO38_000483"
     CDS             complement(5943..6122)
                     /locus_tag="IWO38_000483"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ0312599.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6169..7992)
                     /gene="cadA"
                     /locus_tag="IWO38_000484"
     CDS             complement(6169..7992)
                     /gene="cadA"
                     /locus_tag="IWO38_000484"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ0312600.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8270..10549
                     /locus_tag="IWO38_000485"
     CDS             8270..10549
                     /locus_tag="IWO38_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0312601.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10642..11289)
                     /locus_tag="IWO38_000486"
     CDS             complement(10642..11289)
                     /locus_tag="IWO38_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312602.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNNVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11468..12187)
                     /locus_tag="IWO38_000487"
     CDS             complement(11468..12187)
                     /locus_tag="IWO38_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0312603.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12318..13469)
                     /locus_tag="IWO38_000488"
     CDS             complement(12318..13469)
                     /locus_tag="IWO38_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ0312604.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKTTGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTYSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDVGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13686..14426)
                     /locus_tag="IWO38_000489"
     CDS             complement(13686..14426)
                     /locus_tag="IWO38_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0312605.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEIFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14430..14786)
                     /gene="rsfS"
                     /locus_tag="IWO38_000490"
     CDS             complement(14430..14786)
                     /gene="rsfS"
                     /locus_tag="IWO38_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ0312606.1"
                     /translation="MIDSKEQLKIAVEAADSKRAEDIIALDIRNVSLLADYFMICSAN
                     SERQINAITEEIIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERSFYNLE
                     KLWSDAPLVDLSEWLD"
     gene            complement(14810..15403)
                     /locus_tag="IWO38_000491"
     CDS             complement(14810..15403)
                     /locus_tag="IWO38_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0312607.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDVEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15387..16034)
                     /locus_tag="IWO38_000492"
     CDS             complement(15387..16034)
                     /locus_tag="IWO38_000492"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ0312608.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16047..16358)
                     /gene="yhbY"
                     /locus_tag="IWO38_000493"
     CDS             complement(16047..16358)
                     /gene="yhbY"
                     /locus_tag="IWO38_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ0312609.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16374..17483)
                     /gene="yqeH"
                     /locus_tag="IWO38_000494"
     CDS             complement(16374..17483)
                     /gene="yqeH"
                     /locus_tag="IWO38_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ0312610.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17480..18007)
                     /locus_tag="IWO38_000495"
     CDS             complement(17480..18007)
                     /locus_tag="IWO38_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ0312611.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18234..19019)
                     /locus_tag="IWO38_000496"
     CDS             complement(18234..19019)
                     /locus_tag="IWO38_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ0312612.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRFDQHELLEKRYKRFRQY"
     gene            complement(19012..19881)
                     /locus_tag="IWO38_000497"
     CDS             complement(19012..19881)
                     /locus_tag="IWO38_000497"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ0312613.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19882..21261)
                     /locus_tag="IWO38_000498"
     CDS             complement(19882..21261)
                     /locus_tag="IWO38_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ0312614.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDHF
                     GNVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21269..21688)
                     /gene="fabZ"
                     /locus_tag="IWO38_000499"
     CDS             complement(21269..21688)
                     /gene="fabZ"
                     /locus_tag="IWO38_000499"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ0312615.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21713..22189)
                     /gene="accB"
                     /locus_tag="IWO38_000500"
     CDS             complement(21713..22189)
                     /gene="accB"
                     /locus_tag="IWO38_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ0312616.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPETMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22192..23427)
                     /gene="fabF"
                     /locus_tag="IWO38_000501"
     CDS             complement(22192..23427)
                     /gene="fabF"
                     /locus_tag="IWO38_000501"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ0312617.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIIGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKRDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23446..24183)
                     /gene="fabG"
                     /locus_tag="IWO38_000502"
     CDS             complement(23446..24183)
                     /gene="fabG"
                     /locus_tag="IWO38_000502"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ0312618.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24195..25112)
                     /gene="fabD"
                     /locus_tag="IWO38_000503"
     CDS             complement(24195..25112)
                     /gene="fabD"
                     /locus_tag="IWO38_000503"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ0312619.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25219..25443)
                     /locus_tag="IWO38_000504"
     CDS             complement(25219..25443)
                     /locus_tag="IWO38_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ0312620.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25496..26458)
                     /locus_tag="IWO38_000505"
     CDS             complement(25496..26458)
                     /locus_tag="IWO38_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ0312621.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26468..26908)
                     /locus_tag="IWO38_000506"
     CDS             complement(26468..26908)
                     /locus_tag="IWO38_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0312622.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27214..28179
                     /locus_tag="IWO38_000507"
     CDS             27214..28179
                     /locus_tag="IWO38_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0312623.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRAQLPDLQ
                     EMTFEQKKKELQGLFQGQNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKHFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMIASADVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQSEIELLNKKEAEKQLN"
     gene            complement(28236..28466)
                     /locus_tag="IWO38_000508"
     CDS             complement(28236..28466)
                     /locus_tag="IWO38_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ0312624.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28552..29439)
                     /locus_tag="IWO38_000509"
     CDS             complement(28552..29439)
                     /locus_tag="IWO38_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ0312625.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29528..30751)
                     /locus_tag="IWO38_000510"
     CDS             complement(29528..30751)
                     /locus_tag="IWO38_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0312626.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30768..31847)
                     /locus_tag="IWO38_000511"
     CDS             complement(30768..31847)
                     /locus_tag="IWO38_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0312627.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYSIRVPIQAISNGIDLEKYRPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(31978..33705)
                     /gene="ptsP"
                     /locus_tag="IWO38_000512"
     CDS             complement(31978..33705)
                     /gene="ptsP"
                     /locus_tag="IWO38_000512"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ0312628.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRVLAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(33705..33971)
                     /locus_tag="IWO38_000513"
     CDS             complement(33705..33971)
                     /locus_tag="IWO38_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ0312629.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            34450..36684
                     /locus_tag="IWO38_000514"
     CDS             34450..36684
                     /locus_tag="IWO38_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ0312630.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            36832..37152
                     /locus_tag="IWO38_000515"
     CDS             36832..37152
                     /locus_tag="IWO38_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ0312631.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(37357..37581)
                     /locus_tag="IWO38_000516"
     CDS             complement(37357..37581)
                     /locus_tag="IWO38_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAQ0312632.1"
                     /translation="METEWQIRERKKQKKEEMLRLCRLLDLKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(37779..39359)
                     /locus_tag="IWO38_000517"
     CDS             complement(37779..39359)
                     /locus_tag="IWO38_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ0312633.1"
                     /translation="MNNPELKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(39760..41136)
                     /locus_tag="IWO38_000518"
     CDS             complement(39760..41136)
                     /locus_tag="IWO38_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ0312634.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(41264..42382)
                     /locus_tag="IWO38_000519"
     CDS             complement(41264..42382)
                     /locus_tag="IWO38_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0312635.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            42524..42790
                     /locus_tag="IWO38_000520"
     CDS             42524..42790
                     /locus_tag="IWO38_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0312636.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGMPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILLKMLACLIFI"
     gene            complement(42882..43415)
                     /locus_tag="IWO38_000521"
     CDS             complement(42882..43415)
                     /locus_tag="IWO38_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ0312637.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(43509..44687)
                     /gene="mutY"
                     /locus_tag="IWO38_000522"
     CDS             complement(43509..44687)
                     /gene="mutY"
                     /locus_tag="IWO38_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ0312638.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(44680..45477)
                     /gene="recX"
                     /locus_tag="IWO38_000523"
     CDS             complement(44680..45477)
                     /gene="recX"
                     /locus_tag="IWO38_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ0312639.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNRGKSQKELLRKIQQTLMTKGFSQEVIQQAMADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIERFITEKEEESE"
     gene            45584..46966
                     /gene="rlmD"
                     /locus_tag="IWO38_000524"
     CDS             45584..46966
                     /gene="rlmD"
                     /locus_tag="IWO38_000524"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ0312640.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMICEINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(47140..47718)
                     /locus_tag="IWO38_000525"
     CDS             complement(47140..47718)
                     /locus_tag="IWO38_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ0312641.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            48040..48510
                     /locus_tag="IWO38_000526"
     CDS             48040..48510
                     /locus_tag="IWO38_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ0312642.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(48618..49844)
                     /locus_tag="IWO38_000527"
     CDS             complement(48618..49844)
                     /locus_tag="IWO38_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ0312643.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(50598..50798)
                     /locus_tag="IWO38_000528"
     CDS             complement(50598..50798)
                     /locus_tag="IWO38_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0312644.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(50932..51540)
                     /locus_tag="IWO38_000529"
     CDS             complement(50932..51540)
                     /locus_tag="IWO38_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ0312645.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(51625..52095)
                     /locus_tag="IWO38_000530"
     CDS             complement(51625..52095)
                     /locus_tag="IWO38_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ0312646.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(52143..53057)
                     /locus_tag="IWO38_000531"
     CDS             complement(52143..53057)
                     /locus_tag="IWO38_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ0312647.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            53203..54006
                     /locus_tag="IWO38_000532"
     CDS             53203..54006
                     /locus_tag="IWO38_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ0312648.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            54184..55131
                     /locus_tag="IWO38_000533"
     CDS             54184..55131
                     /locus_tag="IWO38_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ0312649.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(55199..55492)
                     /locus_tag="IWO38_000534"
     CDS             complement(55199..55492)
                     /locus_tag="IWO38_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ0312650.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(55520..56185)
                     /locus_tag="IWO38_000535"
     CDS             complement(55520..56185)
                     /locus_tag="IWO38_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ0312651.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(56325..56957)
                     /locus_tag="IWO38_000536"
     CDS             complement(56325..56957)
                     /locus_tag="IWO38_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0312652.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(56964..58031)
                     /locus_tag="IWO38_000537"
     CDS             complement(56964..58031)
                     /locus_tag="IWO38_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0312653.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            complement(58028..58759)
                     /locus_tag="IWO38_000538"
     CDS             complement(58028..58759)
                     /locus_tag="IWO38_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ0312654.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(58927..59406)
                     /gene="greA"
                     /locus_tag="IWO38_000539"
     CDS             complement(58927..59406)
                     /gene="greA"
                     /locus_tag="IWO38_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ0312655.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(59542..60717)
                     /gene="mltG"
                     /locus_tag="IWO38_000540"
     CDS             complement(59542..60717)
                     /gene="mltG"
                     /locus_tag="IWO38_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ0312656.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            60882..62042
                     /locus_tag="IWO38_000541"
     CDS             60882..62042
                     /locus_tag="IWO38_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ0312657.1"
                     /translation="MSKGKHRKKQKISISFLVILLLSVSFFSTGIIIGGQLTKIPHSQ
                     TYKAEPTQLTSNLTPDEQRLASKIDQLLQKSDYIGSIYVRKNNRVLLEKGYGYANLRV
                     KESNSPSIYYQIGSIQKAMTALLILKQIQAGKLTFDTKLAEFYPQIPGSQQITIKNML
                     YMRSGLHRTASPDKPMSDSEIIQFALHHLKLTDYDTYHYEPLNYTLLTGILIKLTNQP
                     YETLLKKEIIRPLHLEHTDFYDNVKNSPQHAVSYQMSATDDYSKALIEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGGIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVVAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVHLSN"
BASE COUNT        17929 a        13127 c        10490 g        20619 t
ORIGIN      
        1 ttttgtcaca accttttttg ccgagtaaac ggtgttcaaa agccagtttc tcggtatgaa
       61 gtcaaattta agcaaaaatg tgagaagcta ttttcacaaa atttttcttc atactcgaaa
      121 ctaagcggct ttttcacgac ctctttttta ttttgccatg aacatatcat aatacgcgtc
      181 cattatttct ttagcaatcg tcaagtttat gtggtcttgt tcgtctaata agttcggcaa
      241 gacaacactg atggcaattt ccggattgtc tgttggtccg tatgccacga tattactatt
      301 gactgtcgca atatctggat gaccttccgc aaccgtttct gcagtccctg tttttgcgga
      361 aaggtccatt ttcgcactgg ctaatggttt ggctgttgtg taaggatcgg taccatggac
      421 aacttggtgg aatccttcac ggaggatcgc catattttct gcagaaatat tgaccgtatt
      481 ttctactgtt gtgccaattt ctttcttcac ttcacccaag tttccatttt cgtcattgcc
      541 atagatcgct ttgactaggt gcggctgtac tcgtttcccg ccattagcga ctgttgaagc
      601 atattgagct aattggattg gtgtatatgt gtcaaactgt ccaaatgcca aatcggtaaa
      661 gttacctgga gcataccatt ctttgccatt atccgcattg aacttcttca tataatcaac
      721 agaacgtgag atcccaggag attcattcgg caaatcgatc ccagtcgttg taccaagacc
      781 aaactctttg aaagagtctc tcaattgctg ataagcttta gcttcttcat ctaaactagg
      841 tagtcccatt ccaggtgtat agtctagtcc taacatcttt aacgccactt tgatcatata
      901 cgtattagaa gacaattccg atgctttgac tgcatcaaga gcaacttgtc cgcttcgatt
      961 aaagacagag cttttttctg aagcaccttg cagacgaatc ggctcatcaa tcaaaacttg
     1021 atttccgctg atcgcgttac ttgcccagcc tgctgtcaaa gtaccagcct tgactacaga
     1081 ccctggatca aaggcgctag taatcgtgcc taaagcattc tcggtgattt ctttggaacc
     1141 ttgttcatga ctgaaaccag tcattgctag cacttctcct gtctgaggat tcatagccac
     1201 tgcataagca ccaggcgaat actgtgcttt gccgttgttg atcaatgttt gataattcct
     1261 tttcaaaatt tcttctactt ttgattggaa ttctgcattc atagataaca ttaaattaga
     1321 acctttttcc ccagagaaga tttctttttg attcgaaaca tttccttcat tatcaaggga
     1381 aacttcatat tgggttttgg ttccttgcag gacatcttca tattgttttt ccaaataact
     1441 ttgtcctaca cggtcattcc gagaataacc ttttgctagg tatttctctg cttcatctgc
     1501 tggaagtcct tgcttttcag tcgtcacact gccaagaata cttttcagtg aacttgctgc
     1561 attatattct cgtgtccagt ctgtgccagt cgaaactcct ggaagttcag aagcgcgttc
     1621 tgccacaaca gctaactctt gatcagtaac accagagttt ttgatataga cagtgtttaa
     1681 ttcatatgca ctattcatcc gtttaaagat ggtagcaatt tttaattgct cctcgtttaa
     1741 attaatctca tcagctgtga ctttattgac catcgcacta tattgttcgc tgttccctaa
     1801 ttgcttttct tttgccgaaa gtctttttgt ggcttctttc aaatgtttgg gatcagccag
     1861 ccaaaaatct tttttatctc tatctgtcag attttcatct accagaacat cgatcagcga
     1921 gttcagcttc gttgcgattt caagcaaatc tttagccgta acttgtgttc ctctcgtata
     1981 tgtgatggcc tgattcgctt tattcgtaac taaagctttt cctgtggagt cgtagatcat
     2041 tccgcgagga gtactttctt ggaccgtaat cgtagaagta gacttcactc gcgcctccat
     2101 ctcttcgccg ttcacgatct gcaaataacc caatctagca atcaacgcaa caaataacgt
     2161 aaatataatg aaaaaaagaa aatttaagcg aaaaggaaca tgggaccggc gaaacgtttt
     2221 gttcttaggt tcttgtgcac cttttcttat tttttctaac caagaagtat ttaatatttt
     2281 tttcataaaa tctttcatca tttcctatcc ttccgcctat ttacattcat gcttcattgt
     2341 acccaaaaac ttcttaataa aaaagaaaaa atgggcggag caagaaattc tttacctaat
     2401 ttcaagataa aatcaacttt ttgtttcaca gcaatcactt aaagttacat atccaactat
     2461 tttttcatta aaaaataaac ttatttttga aaattatgtt ttcttaagtc gtgaaacact
     2521 ttgatggatc atttatttgt ggaaaactac gttttgttat tctaaaaaac ttatataaaa
     2581 aatagtaagg tttttatttc gaatccataa aatattcttg gattgcttct cgtgtttttt
     2641 tcactttttc gctaagcttt tcgtttttag ccatatagaa ataaaaaatg atatcgatcg
     2701 tagcaaattg ggccaaaagt gaattagttg ccgcacttcg attcgtgatt tcttttggtc
     2761 tagatgtttg tacgagtaca tcggctatct tgctaagcgg gttatttcca aattgcgtca
     2821 acgcaatgat ttcgatattc atgcttttcg ccagctcggc taatgcgact acatctgcac
     2881 ttctacctga gtttgaaaca agccaaagca ctttcttttt ctcattgctg acaagttgcg
     2941 ggagcaagac gtggatatct ttttcaaaaa tgattggctt accgacacga gaccattttt
     3001 gcagaatatc ttctactact aaagaagagg cgccaacccc atataaaaaa acagtctctg
     3061 ccgattccaa acactctacg actttttgga tcgtttcttc ttctagaata tctactgttt
     3121 cttttatcgc tacttgtgca ttgttaccaa gtttgttttt gatagagtaa aaggactcat
     3181 ttgcttctat atcactaaaa ccttcatcga ttccagtctg tagttctgtt gaaatcgtga
     3241 ttttgaagtc cgtcagactt tgaaaaccga tttttttgga aaaacgtaca actgtcggcg
     3301 cactcaaccc ggaagcttct gctaattcat tagctgtcat tgtagggacc ttctcaatat
     3361 taccgataat gtagttggcc aacttttttt ctgctgaaga ataatcttcc atcatacttt
     3421 caatacgact aattacgctc ataagttcct ccaccctttt aatgatagtc tatgtaagta
     3481 taccatatca gacccttatt cacggtcgta tactggataa aaaacaaaaa aacaatccgc
     3541 ccaacagaat atcttatccc atcaatccca cagattctat tgagcatctg tgtctttgag
     3601 gaatacctgt tatctattgt tcggattgct ttctatctac tactttttta caggaaattt
     3661 acctattagt tttctctgct gtaatttttg acttctgctt ccatagcatc aataaagata
     3721 ttcaaatctt ctacagctgt ttctttactt ccataacgac gtacgttcac tgttgcatct
     3781 tccaattcct tgtctccaac aactaattgg taagggatct tttgtgtttg agaggcacgt
     3841 atcttatagc ccattttttc attacgatca tcaacttcta cacgcaaccc cttcatttgc
     3901 aaacgttctt tgatttcata agcataatcg ccatgtgcat caacagaaac agggatgatt
     3961 gttgcttgta taggtgccaa ccatgttgga aaagcgcctt tgtatacttc tgttaaataa
     4021 gcgacaaaac gttccattgt agaaacaatt ccgcggtgga taactactgg acgatgcgta
     4081 ttttccccgt cttctccaac atatgtcaaa tcaaagcgct ctggcaataa gaagtctaat
     4141 tgtatagttg ataatgtttc ttctgtacca agagctgttt tgacttgaac atctagtttt
     4201 ggtccgtaaa aggctgcctc gccttctgct tcaaagtaat ctagttctaa ttcatccata
     4261 gcggctttca gcattgcttg ggctttttcc cacattgcgt catcatcaaa atatttatct
     4321 gtattgtttg gatcgcgata gctcaagcgg aaacgataat ctgtaatatt gaagtcagca
     4381 tatactgcca ccatcaattc aagcgtacgt ttaaattcat ctttgatttg atcaggacga
     4441 acaaatgtat gaccatcatt caacgtcatt tctcgtacac gttgtaaacc tgacaaagca
     4501 cctgattttt catagcgatg catcattcct aattcagcga tacgaattgg taattcacga
     4561 taagaatgga tcgtgttttt gtagaccatc atgtgatgag ggcagttcat tgggcgaaga
     4621 acaagcattt cgccatctcc catatccatt ggcgggaaca tgtcttcttg ataatgatcc
     4681 cagtgtccag atgttttgta caactcaaca tctcccatta ttggtgtata tacatgttgg
     4741 taacctagac taacttcttt gtctacgata taacgttcga tcgtgcgtcg gattgttgcc
     4801 ccttttggaa gccagaatgg caaaccagaa cctacttctg gggaaaccat gaacaagtca
     4861 agttcttttc ctagtttacg atggtctctt tcttttgctt cttcacggag acggatatat
     4921 tctttcaaag ctttcttgtc aaaaaaggct gtcccgtata cacgttgcat catttgattg
     4981 tttgagtttc cacgccaata agcacctgcc actgataata atttaaatac ttgtatacgg
     5041 ccagtggatg gaacatgtgg tccacgacac aaatcgatga attcgccttg ttgataagca
     5101 gtaatcactt cttcttctgg caattcacta atcagttcta ctttgtacgg atcacttgca
     5161 aaaatttcta atgcttcctg ttttgaaaga acacggcgtt cgatcggcag attttctttg
     5221 acgattttca tcatttctgc ttcgattgcc ggcaaatctt ctgcagttac ttggttttgt
     5281 ccattatctg tatcgtagta aaaccctgaa tcgatagctg gtccaacgcc aaagtggata
     5341 tttggataga ggcgacgcat ggcttgggcc atcaaatgag ctgctgaatg acggactaag
     5401 ccaagtgcat cttcatggtc tggtgtgatg atctcaatac ttgcatcttc ctcgattgga
     5461 cgagtcaaat caacgagttt tccgttcact ttaccagcta aagctttttt tgctaagctt
     5521 tttgaaatgc tttcagcaat tgccaatgtt gttgttccag actcaaattc tttgactgca
     5581 ccatctggaa aagtaatttt tatcataata gtttcctcct tgaaatgttt tgattgatca
     5641 gatcatcaag ctgaaaaaca aaaaaatcct agcatctctt gaaaagagat actaggacga
     5701 aaagttcgtg gttccaccta atgttcaaac gcaaatgcgt tcctcaagcg ttgtaacggt
     5761 ccgaccgccc ttgcttcaac aagggtttcg aaagtggtca tcctttggtt ttctcctgga
     5821 aaagtttcag cctagctttt cctctcttat cgaagactct caaattcagt tggctttctc
     5881 atcaatcaat tatgtgttta tttaaccact aataaataga aaaatcaagt gggaaactaa
     5941 acttattttt tccattcccc atttacaccg tatgttcctt ttttcatctc attgatgatg
     6001 acatggatgt gttcttttgg tgcctgcgtg tttttatgaa cagcttccgt gatatctttc
     6061 atcatatttt ctaattgttc cggactgcga ccttctacta attcgacatg tacgaatggc
     6121 ataatgctct ctcctctttc tgatcgttct tttttcaact cgattctatc atactatgtt
     6181 tgtcttgggt actgaatcag gaaaaatggc tggatctttt tttgctctca aggcactcaa
     6241 aatagagacc gtatccacga cttcttgcaa aacagctccg aataatgcgg ggattagtcc
     6301 cgtacttgca attaacatca aaaaaataca aataaagatg ccaatcagta cagactgttt
     6361 cgctattctc atcgtatctc ttgaaatcac tactgctttt gctacacgca agagatcatc
     6421 tttcaagatg accgcatctg cactttcact agcagctgta gaaccatgag cccccattgc
     6481 aataccgata tctgcagcag caagtgctgg tgcgtcattc acgccgtctc ctaccatcgc
     6541 aacaggtctt tgatctttag ggatactttc taaaatggtc agtttatctt gaggcaagct
     6601 acgggcatgt acagaatcta attggacttt ttctgcaatc gtttgagcaa tggcagatcc
     6661 atccccggtg atcatcatca aatttttcac atcaagtttt cttagctcct tgattgtctt
     6721 tgctgcttct gggcgaatct gatccgaaaa taggatcgtt cctgcaaaag atcgatcaat
     6781 tgttacatag attgctgtat tggacgtttc tatttcttca gctgaaacaa aagaggcacg
     6841 tccaatcttt acttcatgat tattgactac tgcttggatt ccttgaccgg tcacttcttt
     6901 cagttgacta acaggataca gttggctgtt tttcacgttg ttcactaacg aacgagctaa
     6961 gatatggcta gattcctgtt ctgcactagc tgcatacaga agtagctctt ctttttttat
     7021 tccattgact ggctgaatcg cctctacttc caatatccct ttggtaagcg ttcctgtttt
     7081 atcaaaagca atcgtttttg tacgtgcgag cttttcaatc gttgttccag tttttacaac
     7141 gatcccgttt ttacttgaac ggctcattcc tgcaactaaa gcaatgggcg cagctagaat
     7201 cagtggacaa ggtgatgcca ctaccagtac ctcagcaaat cgtaaagggt cttttgagaa
     7261 ccaccaagaa ataccaccga tcaaataagc aatcaacgtg aaagggactg cataacgatc
     7321 agctaaacgg acaaatcggg caggcttttc ttttgattct tcgactagtt taataatcat
     7381 ttgatactgg ctgtctgcgg cacgtttttc agcgatcatt tggatcgatc cgtctccatt
     7441 taaagaccct gacataatct gatctccagg tttcttggtt tccgggcggg actcacctgt
     7501 caaagaagat tcatcgaccg aagattctcc tttggtcact cgaccatctg cgggtacaag
     7561 ttcgccaggc ttgactagta actggtcatc aatatttatt tcatcgatta ctatatcctg
     7621 caattttcct tctttgaatc gatgggcaat tcttggggtg ttatctaaca aagactgcag
     7681 ttctttaccc gctcgtttat tcgcgtaatc ctctagacta tctccgcccg taagcatgat
     7741 caatatcatc aaactagccc agtattcttt gacggctaat gttgcaagga ttgcggtaat
     7801 cgctaataca tctacgccat atcttcctga acgtaacgtc ttgatcattt ccaaaaacat
     7861 caagaaggcg agaatccctc cagtaaatgc gacaatcgca aatgcaaggg ccggctggtg
     7921 aaaaacaaat tcacttaata aagctatccc accaataagc aaagtaagta aaaatttttt
     7981 gaaatgtgtc attgtttttt ctcctctttt atgtgactta gagaggttag taataaattt
     8041 ccagctgcct ctttcatctt aagcatagtc cttgactagc actttttaaa acaataggcc
     8101 ttaattgaaa acaactatca ctatcagcgt ttccaaaatt catcagttct acaaacaaaa
     8161 atattcagca aggattactt tttcgattca tcagttaaaa agtacctttt ttcgaaaaac
     8221 tatgctatac ttttaattcg ttatttacgg cggaaggagc aaacatgcga tgaatgagaa
     8281 acaactagaa caaacgcacg tggatgaaac gatcgaacta atccagatcg aacaaaatat
     8341 gctggataca aaacaacaag aattgactca ggaaatgaat cattcgacaa aagacacagc
     8401 taatcacaag attcgagctg gttccaatga agctttttat gaatctgctg ttgaatatcg
     8461 ccaacatgag caggaattgc ttttgaaata ccatactctt gaagcacagc aaaaacgcct
     8521 gcaaacgttg tctgtcatga aagatagtcc ttattttgcc cgtatcgact ttaaagaaga
     8581 ccgtgaaaca gaaacattat atctaggtat cgcttcttta agagatctgg cagaagaaac
     8641 aatcgttatt gactggcgcg caccaattgc caatctttat tatgaaggtg agattggtcc
     8701 cgcattttat gaaactgaaa cagatagaat agatgtcgat ctgcagttga aacgacaatt
     8761 taaaatccaa gaaggacgta ttttatctat ggtagatacg tcggaagtaa tcaatgatga
     8821 ctttttgcta gagatattgg atgaatcatc aagcaaccat atgaaaaaca tcgtctctac
     8881 gatacaaaaa gcacaaaatg caatcattcg ggacacaaca agtaaagtaa tgctgattga
     8941 aggaatcgcc ggaagtggaa agacatccgc tttgcttcaa cgtattgctt ttctgttgta
     9001 tcataatcgc aagtggctcg aggctgaaaa cgttcttcta ttttcaccca accatttgtt
     9061 ttctgattac atctcgactg tattgccttc attaggtgaa agcggcgtgc ctacacagac
     9121 attcaaaaat tatttggtgc agctgctgcc agagttttct ttactagatg aacagcaaca
     9181 agaaagtggt tttttgctag gggaaaaaga tccgatacaa gtgatgaaaa gcggattgac
     9241 cttagtcgat cagattgatc gctatatcca aggcatcaca aactacggtc ccttgttccg
     9301 tgatatgaaa ataaatggcc gtacgatcct atcaaaggaa tcgattcgcc aatggtataa
     9361 agaaacaaat gaacaattac ctttacatca tagactttct ctattacaaa cgaagctcct
     9421 gaaaaaatta ggcggtctgc aaaaagacga aacacgtcag caatgggtca aagatctggc
     9481 ggaagaacag ctccaagaac tatacgcaac agatccaaat cttgaatata cagaacaaaa
     9541 ggagcgttca ttacgtaaaa aactggccaa ccaaatcgta cgtaaaagat tccgtcgaat
     9601 ccatcgaggg atcatgaatt atcaatttgt caatataacg aaacaatatc tccactttct
     9661 acaaactgtg ccactttctc ttctaacacg acataaaatc agcgagacca ggtggcagga
     9721 acatttgaca actgttcgta cacacttgcg tcaaagagaa ttgcgtcaag aggatgctgt
     9781 actattcttt ttactgatgc gccgaacata tccagtgacg gttacacaca aagctcgcta
     9841 tatctttatt gatgaaatgc aggatttcgc accatcacaa gtggcattgc tgcaagcctt
     9901 gttcccaaaa gcaaatttga ctttctgtgg agatctaaat caaaatgtct ttggaaatga
     9961 aacgatcgtt ggatcattgg aagacctttt cgtggaccgt gaagtcactc gtttccaatt
    10021 aacgacaagc tatcgctcca caaaagaaat cactgatttt gctaatcatt ttattgtgaa
    10081 agaacatcaa gtagaaacaa cagcaagaaa aggagacctt cctttgctca tccaaggaac
    10141 aacagatgat atcaaattga attggttagc agataccatt cgaacgactc agggaaaagc
    10201 ccggtattgg cgtacagcaa tcatagctaa aacacaagaa gaatgcgaag ctctttatgc
    10261 tcaactgcca gacgagttga aacaacaagt ccagctgatt ttaaatgaga gtgactttat
    10321 gaaacgttcc attattttac ttccagcctt tttagcaaaa ggtctggagt ttgaccgagt
    10381 tttcttgtgg gatgtagatg cagaacattt ttcttctcag caagacaaat tgatccttta
    10441 caccatgtgt acacgtgcga tgcatgaatt gacccttttt acctccaata atgaaagtcc
    10501 tttgttaaac aatatggata tttcatctta tgagaaaaaa gtactgtaag taaaaagaca
    10561 actgctatca ttaatgttag cagttgtctt tttatttaca ataataattg cagaaaaagt
    10621 aattcaatca tttaaggtca tttattctat atataaatcc aaatctgttt caggatattt
    10681 taatttgaca agcgaaataa tcattttcag ttcttccatg ttttctggat attctgggtc
    10741 aaataccttg ctatgacggc caatcccgcc attgtcgatc aatcggtaaa ttcgattcca
    10801 ttcaatgaat tggtcaattg attggaaatt gctgattgga tagccgccac aatccaaaat
    10861 ctctgataca cgaaatgcta ggtatgcact atcgatcaca ttattttttt tcgtgtctat
    10921 cttctcattt ctttcgataa gaattttttc aggacgatgc ttcaagctcc actctaatag
    10981 tagaaagaaa gcctccatcc gatactcttc ttcaaataac cgaaaggctg aatcagaacg
    11041 acgaaaaagc agcggaatca gataattttg tctataaatc agcattggtg cgcggtccac
    11101 catacactct aaataacggt tcagataccc tgtaccgaca tagtttccta atcgatagcc
    11161 ttcaacgccg aatcttgcgt atttttgcgc taattttgaa caatcagtta cgttcacagc
    11221 taactcttct actttgcggt aaccgcttaa gatacgttca aaaggttcta aagtgatttt
    11281 tgcttccata ttcttccccg tccatcttcc acatacttca tatcatttca catgatactt
    11341 atgctctcat cataccacag gttgaattag aaaagaaaat aaatcacaca aaaaagcaag
    11401 acaatctgtt agtgagattg tcttgctttt cgaaaaagac tgaatacttt tcgttaaaac
    11461 tggttgctta gtcttctaat gccacattat ggaacacatg ctgtacatct tctagatctt
    11521 ccagtgcatc aatcattttt tcaaatgttt cttgatcttc tggagatagt gttacctcgt
    11581 tttgagggat catttggatc tcagcgattg aaaaatcagt gattcctttt tcttttaatg
    11641 cttcttgtat actatggaaa tccgctactt caccgtaaac gatgatttgt ccttcttctt
    11701 ccattacatc gcgaacatcg atgtcttttt ccattaggta ttcaaggatc tcgtcagcgt
    11761 cttctcctgc aaaagcaaag attgctgtat tgtcaaacat gtaagaaacc gctccggaaa
    11821 cacccatgtt cccgccattt ttcccaaatg ctgcccgtac gtctgctgct gttcgattca
    11881 cattgttcgt caatgtatca acaatcacca ttgagccgct tggtccaaat ccttcatagc
    11941 gcaattcaga gtatgtttca tctcctgcac cttttgcttt ttcgattgct cggtcaataa
    12001 tatgttttgg tacattataa gttttagcgc gatcaatgac gaaacgtagt ttttggtttg
    12061 catgcggatc aggatcacct gattttgctg ccgcgtagat ttcaatccca aattttgcat
    12121 aaatccggct attattagca tctttagcag ttttctttgc tacgatattt gcccatttac
    12181 gtcccatatt ttcacttcgc tttctgattt gatagtttca taacgcaaaa acgtcctgtt
    12241 ccattatact tgtgaatcac gattctgtga agcttttttt ctttcttcct tcattcttct
    12301 catttttagt caattatcta tactcggatt gggatttgcc cgaaattctg ttccggaata
    12361 ttgcctccca attgatagat ttgatcactt ctaactaaca gttccgcttc ttgtgcctct
    12421 ttttttccga ttcgggaata aatagaaatc tctttttttt cttttaagaa tcttcttcca
    12481 acatcggtaa atccaagtag tcgaagataa tttttatttt gttggacagg aatctcttgc
    12541 tttttgatat tcagcaagac ataacaagat aatcgttgga tccgtgtcca tgtatatctt
    12601 tttgttttta cctttgacac aaaatcctca aaactatcag cagttgcagc tgctgttttc
    12661 atgcgcgatt ctattccttc ggtcatctga tagatatctg tcagttccag caaagtagaa
    12721 gataacaacc gataacgcaa atacggccaa taagattccc aagtctgcag cgaataagtt
    12781 gaaagatctt cttctgtctg aggcggcaca agtccagcta ttctttttcc ttcttctaac
    12841 gcatggcgaa tggctgttgc actggctatc ctctgatctg tgatgatttt ttcatgatag
    12901 cctgcacttt tacgtcggat cggatgtaat tccatagggt ggacatactt ggcattttcc
    12961 tttgcataac ttaatcccaa gatatgattt ggcgaagaaa aatcaagcgt aatatcggga
    13021 tacagactgc gaaaaacctc agtcattttt tgcggataac tatacgtttg atcagacagt
    13081 tcttgaaatg cctcttctat cctatcttga ttctcttgga caaagcgtcc gaaactgctg
    13141 taatcaaacg aagtctcttc ttctgttcca aaacacaaac tgtcacagtc cagtgtttgc
    13201 aagattttta ctgcaccttt agcaaaatag tcagctgact gaaccgccca ttcgacggga
    13261 agctccacta ctaaatccac accattttgt aaagcttcat tcgcgcgatg ccatttatcg
    13321 atgatagccg gttcgcctcg ttgcaaaaaa ttcccgctca ttacagcaat gacgacctct
    13381 gctcctgttg tttttcgtgc catttccaca tgatagcgat gtccattgtg aaaaggattg
    13441 tactctacga taatcccgca gcttttcatg cgcatcctcc ttcatagtta tcgataaaaa
    13501 agaaatagag acaagtaaaa aaaggcaaaa atcggaaaaa atacccgaac tatataaact
    13561 attctcgcaa acacaatact tctgcgacga aaagctgcac tgcaagagca atgcctatat
    13621 cgttcggata tataatttat gttgaagatt tattcttttc ttgttaactc gagcaggcaa
    13681 aaccgctagt ctttgtatgc tacaaagaac cagcgctttg atttctctga tgctgcttcg
    13741 tctgtaaaat cagaaaatgc ttcgactttc gaaaagccgc tgttttccag cattcttaaa
    13801 taactttcca atggataggt acgctcttga tgtagttcat cttcacgaat aaatcgacca
    13861 gacccttctt gttcttcaac gaaaaaggta agaaaatgtt cgatgctgtg tttctcgctt
    13921 cctggataac tatcccacaa aaaagcaaaa ttatcccctt gataatgata gctatattct
    13981 ggaaaaattt catctacttg ataggtggag tgtacgtcaa ataaaaatcg gccattttct
    14041 tctaacgctt gatagacttc atcaaacact tgctgtacct cttgtctatt tttcatatag
    14101 cacagcgaat cagaaaaaca ggtaattgcc tgatattgtc cgatttctga taaatcgagc
    14161 atattgcctt ccacaaattg gatcgatgcg tcttcttcat atgctcgctg gctggcaatc
    14221 atcaacatct cttctgaaag gtctaaccct gtcactttat atccatcttt tgcaaaggca
    14281 actgctaatg ctccagtacc gcatgccagt tccaaaatat tttttgtgtc ttcttgtaaa
    14341 tgacgtttcg aaaaagctag ccattgctga taaagtgttt cgtccatcac ttcatcgtaa
    14401 atgaaagcaa atgtttcata agccatatgt taatccaacc attcactaag atcgactagc
    14461 ggagcatctg accatagttt ttccagatta tagaaacttc gttctggtgc atggaaaaca
    14521 tgtacgatca catctccaag atcgatcaat acccattttc cgccttcttt tccttcaata
    14581 cgttttactt catatttgtt ttcttcttct ttatcaatga tttcttctgt aattgcgttg
    14641 atttgacgtt cgctatttgc tgaacagatc atgaaataat ctgccaacaa ggaaacattt
    14701 cgaatgtcta aagcgataat gtcttctgcc cgttttgaat ctgctgcttc aaccgcaatt
    14761 tttaattgtt ctttactatc tatgatgatt tcctcctaat atgagcagct tatttttttg
    14821 ctacccagta attatatgtt tcgattgttt ttggataaat ctgttgttct tgcgcaagca
    14881 aatgggccaa tgtatgtttt gtttcaaatg ctacagcttc gtctagatca gcccatgcaa
    14941 tggctcgtgc atcttgtacc ccaggaaaat ctcgacctgg ttcaatatag tctgctacat
    15001 aaatgatttt gtcaaggaga ctcatcttag cagccccagt cgtatgcacc cggattgcat
    15061 gaagaatttc tacatcggtg attcctaatt cccgctctac aaacgaagcg ccaacaagac
    15121 catgccagat tgcattatta taattcaata actctggatc aaaaccgtct cttacgatta
    15181 ccattttgaa ctcctcatct ggtcgttctt ttgcataatc atgtgtcaat gcagcaatac
    15241 tagctttttc aggagaagca ccgtattttt tggcaagagc cacggctgtt tcttctactc
    15301 caagcacatg cttgaagcgt tgttcgctca ttctcatctg tattttttgc atcaggtttt
    15361 ctctagaata agcagtatat tcattactat agtccatatt catacaaccc ttcattttga
    15421 atataatcaa ttactttatc tggaacaagg tagcggatag aacagccttc tttgatcttt
    15481 tgacgtattt tcgtggaact gatgtcgatc tcaggtacat ctacccaaat tacaggatat
    15541 ggagtatccg ttgtgtatcc tggtcgacgt atacccacaa agttgaccat agaagtcagc
    15601 tcatcgattt tataccattt tggcaaatat tctaccatat ccccaccgat aataaagtaa
    15661 taatctgtat ccggattgtt ttgcgtcaat tccttcatag tatcataggt gtaacttttc
    15721 ccaccacgag ctaattcaat cgtttcaatc tgcaaaaaag gattgtcttc cacagctaat
    15781 tccaacatgt tgagtcgatg ttttgcatca atcgtctgtt tctcatcaac atgaggtggc
    15841 tgataagaag gcatcaaaaa tacgcggtca agacctaaat tccggccagc ttgttcagcc
    15901 atcactaaat gtgcaaggtg aacgggattg aatgttccgc ccaagatccc tacttgcttt
    15961 ttcttctcta agaattgagg catttcttca ggaaataatt gtgaacgaac aaatgtctta
    16021 gcttgcgtat tcatcatggt cactccttat attgctttta cttcttttga tagcctttga
    16081 tacttttctt ttgtggaagg tttgtataat acaataacac gtccaatgat ttggacgatt
    16141 tcgcaatcta tttctttttc taatacttga gctgcttctt ctgcaatctc atctgtattt
    16201 tgaagtaacg atactttgat cagttctctt ttttctaaag cttcttcaat ttgaacgatc
    16261 attgctgaat tgatcccacc ttttccgatt tgaaaaatcg gctgaagatg atgcgctttg
    16321 ctacgtaaaa aacgcttttg ttttcctctt aaactcataa ttttccttct tccttaaatc
    16381 aatgctttgc gtttcaatac atctacaccc tttggcgccc agcccgcaat tactactggc
    16441 gctgttacag tgatccagcc tagaccggca aaaacgatgt ctgttttttc tttcacagaa
    16501 aactcaaaac gaacaagctc cggaaatgcc tcgacttcat caggacgagg aggctgtaat
    16561 aatccaccta catgtttttg ataaaattcg tctgcttttt ccagtttcgt tcgatgaata
    16621 tttacatcat ttgacacata agcaataaat gaagcacgat tgccgctgat aaaatcaaat
    16681 cttgcaagtc cgcccaagaa aagtgtttgc ccttcattca gctgataaac tttaggtttg
    16741 atttctttta acggtgcaat gatccgcaaa tcttttttac ctaagtagtg cgccatttga
    16801 tggcgatgga taattcccgg tgtatcaatc aaaaaatgcc cgtcatctaa aggaatctcg
    16861 attttatcca aggtcgttcc tggaaactgc gaagttgtga ttacatcttg tactcccgct
    16921 gtatttttga tgatttgatt gataagcgtc gattttccga catttgttac tccgacgata
    16981 tagacatccc gtccatcgcg gtattcttcg attttttcta atagttccgt catctcatga
    17041 gatttcttag cactagttaa taatacatct actggacgaa ttcctgcttc gtaagcacgt
    17101 tccttcatcc attgtgttaa tttagttttt ttcaatgatt taggtaatat atccactttg
    17161 ttcccaacta aaagtactgg attgtcaccg acaaaacggt gtagtcctgg gattagtgaa
    17221 ccattgaagt caaaaatatc caccacattg acgatcaatg catctttctg ccctaattca
    17281 ttcagtaagc gcaaaaagtc gtcatccgtc aatgagacat cttggatctc attataatgg
    17341 cgtaatctga aacaacgctg acaatagact tccccggaag ctaaaccttt ttccaaagcc
    17401 gtttgcggcg tgtatcctaa ttcaccgggt ttgtttgttt gtataacggc accacagccg
    17461 atacattgta attcttctgt cattcgattc cacctcgcca gcccatatca gggtgttttg
    17521 ctaatagata ggccataatt cttttttccc aaaaacgatt aaattgtgtc ttccaactat
    17581 ctgttgcgat aatgggtttt acaaggatgc tacgtatccc tgcacgattg gctccacgta
    17641 tatcagtcat aatctgatcg cctaccatta ctatctcatc atctgctaaa tccaattgtt
    17701 tctttgctat gttgatccct cttgctaaag gtttcattgc tcgtgcaaca tacaaaagat
    17761 caaatttttc aatcgctcgc gcgacacgtt ttgaattatt gtttgaaaca acaacaacag
    17821 ggattcctgc atttttcatt tctaaaatcc aagtaagcaa ctcttctgtt ccatctggat
    17881 tattccatgc aatcaacgta ttgtctaaat cggtcaatac tgctttgatg cctagattct
    17941 ttaattgggc aggtgtaatc tgatagatgg cttctaccat ccatgttggt ttgtaatttg
    18001 aaaacatgct gtctcctcat ctgcaaaaaa tgaaggagca cataaagtac tcctcaaccc
    18061 cgtattatac tgcatatcag caaaaaaaac tacctctata ctcgttcgtc tttcagaaaa
    18121 agcatttctt tacaaaatga ccaaaaataa agattgatca cagaaaaatt tagaaacaaa
    18181 aaacaactaa gcgagcaaga ccattcttta ctctttgctt agttgttaaa cacctaatat
    18241 tggcggaacc tcttgtatct tttttccagt aattcatgct ggtcgaatcg aacaagttcc
    18301 gtcaatcttt caatcaacgc ttttttcata atgtgtgcga ctttatatcg tggcaactgc
    18361 tcgccgttga acgtttcagg aataactcga tcaacgattc ctaactcgcg tagttctggc
    18421 gcagtaattt tcatcaactc agctgcttct gcggcgcgac taccgtcttt ccacaaaatc
    18481 gacgcaaatc cttctggaga aagcacagca taaatcgttt cttccagcat ccagacttca
    18541 tccgctaaac ctagagcaag ggcaccgcca cttccacctt caccaatgat gatggcgatg
    18601 atcggtacct ttaaatcaga catttcgagc agatttctgg caatagcctc tccttcgccg
    18661 cgctcttctg ctcctacacc gcaatatgca cctgctgtat tgatcaatgt aatcaccggt
    18721 ctattgaact tttccgcttg tttcatcagt cgtaaagctt ttcgatagcc ttcaggatgg
    18781 ggagaaccaa aatttctgcg gatattttcc tgcagatccc gtcctttttg aataccgatc
    18841 actgttaccg gtttgccatc taatcgagcg attccaccaa caatcgcttc gtcatctgca
    18901 aacaaacgat caccatggca ttcctcgaaa gaatcgaaga tctcttcaaa aaaatccaag
    18961 cttgtccatc gttctttagc tcttgctaac tgaacgacat catgtgctgt cttatccacg
    19021 atctatccat cctttctgtg tatgcaattc aagaagcgta tggattttct gcttcaattc
    19081 catgcgggga acaatctgat cgacaaaacc atgagataat aagaattcag ccttttggaa
    19141 atcttctgga agttcctgct tgatcgtctg ttcgatgacg cgtcttccag caaaaccgat
    19201 caagctttgg ggttctgcta gaatgatatc gccttccatt gcaaaactgg cagttactcc
    19261 tccggtcgtc gggtccgtta aaacagttag ataaaaaaga ccttcattgc tatgccgttt
    19321 gacagcagca gagatttttg ccatctgcat tagagaaaag attccttctt gcatccgtgc
    19381 gccacctgaa gctgtgaaca agacgactgg aagactttcc tttgttgctc gttcgaataa
    19441 tctggtgatc ttttctccga caatcgttcc catacttccc atgataaaat taggatccat
    19501 cactccaata atgaagggaa tcccttgaat ggttgcttta ccagtgagga ctgcttcatg
    19561 aagtcctgtt ttatcttgca tttttttaat tttttccgta tattcgggaa atgctagcgg
    19621 atctttggtt acaagatccg tatcccattc ttcaaaactt ttttcatcaa taatcaaagc
    19681 tagacgctgc catgcaccga ttcgaaaagc gtatccgcaa tgagggcata ctttttcttc
    19741 accgatgtct ttcgtataaa ggatatgttt gcaatttgga cactttgccc acatgttatc
    19801 aggaacagat ggcgagttag caggtttgct ctctctatta ggattgatcc gaatatattt
    19861 ctttttctta aacagagcca tctatgcgcc tcctatctcc gtttctggtg tccaattcgg
    19921 taaaaattct tcttgtaaaa atgctgtgct gtaatcacct gctatgactg cagggtgact
    19981 aatcaaatcc atctgaaatt ctgcgttcgt gatgattcct tctgtgataa attcacttaa
    20041 ggctcgctgc atcttcatta atgcttcaaa acgcgtattt ccatggacga ttatttttgc
    20101 aatcattgaa tcataatatg gcgggatcgt atatcccggg taaactgcac tgtctacccg
    20161 cagtcccatc ccacctgctg gaagcagtaa gttttggatc ttccctggag aaggagcaaa
    20221 atggaaagca ggattttctg cattaatccg gcattcgatt gcgtgtcctt ggaatactac
    20281 ttcttcttga cacacattca aaggctcacc ggctgctatc tccacctgtt tttttactaa
    20341 atcgattcct gtgaccattt ctgttactgg atgttccact tggattcgag tattcatttc
    20401 catgaaataa aaatcgcctt cttcatccat cagaaactcg atcgttccgg cattttcata
    20461 attgactgct ttagctgctc gaacagcagc gtctcctagt gcttctcttt ttgtttgtga
    20521 gatcactaca gaaggagatt cttccagtac tttttgatta ttacgttgca aagaacaatc
    20581 tcgttctcct aaatgaatga cattgccaaa atgatcacct aaaatctgaa cttctatatg
    20641 gcgagcagga taaatgattt tttccaaata catatcatca tttccaaatg ccgctgctgc
    20701 ttcttgctga gctgaagaaa aatgctgtgg gagctcttct tttgattgaa ctttccgaat
    20761 gcctttcccg ccaccacctg cagctgcttt cagcatcact ggataaccaa ttctgtctgc
    20821 tatttccaat gcttcttcta ctgtagataa tacaccagta cttcctggaa tgactgggac
    20881 atttgcttct tgcatcaatt gacgcgcatt gatcttattt cccattgcat cgatcgttgc
    20941 acttttagga ccaataaatg tgatattgca ctcttcacac atggaggcaa atcggctatt
    21001 ttcagataaa aaaccaaatc ctggatgaat tgcttcggct tttgtcacga ttgcagcgct
    21061 cagtacttcc tgaacattca gatatgaatc aacggctttt gccgggccaa tgcagattgc
    21121 ttcatctgct aactgtgtat gaagtgcttc ggcatctgct tctgaatata tcgctacggt
    21181 ttggatccct agttcgcggc atgcacgaat gatacgtaca gcgatttcac cgcggttagc
    21241 tatcaatact ttagaaaaca tatttcacct atccaatcat aaaggttaat tccgcttcgg
    21301 ctactttttt accgtctact ttagcgattc ctttgcctat tccggctgaa gctttcactt
    21361 tcaatatctc aacttcaagg tataaggtat ctcccggtgt aactttctta cggaattttg
    21421 ctttgtccaa tccgccgaaa taagccgtct ttcccttgaa ttgttcaaga gaaagtaatg
    21481 caactgctcc tgcttgagcc atcgcttcaa caattaatac ccctggcata acaggttctt
    21541 ctggaaaatg tccttgaaaa aagggctcat tgatcgttac atttttctta gcaacgatcc
    21601 gttctccttc aaccatttct tccactcgat caatcaaaag cattggataa cgatgtggaa
    21661 tgatttcttt tatttcttgt atgttcattg atttccctct ttcctgcttt gcttattttt
    21721 gctgaatcct aaagagtggc tgtccatatt cgacaactga ctcgttttct acaaggattt
    21781 ctgtgatcac tccgtcggcc tcactagtta tctcattcat cagtttcatc gcttcaataa
    21841 tgcagataac ttctcctttg gcaacttgat cacctacttt tttgaaagct ggtttgtcgg
    21901 gcgccggttg cagataaacg attcccacga ttggcgaaac aacctcgatt ccatcaaata
    21961 ctgccgattc tttttctttc attgtttctg gagcagttgt tgcttgttca ggtatgacat
    22021 tgactggctc cgaaactggc aacgtttcgt ttgtttgaac catattcatg cctcgacttg
    22081 ttttattttt attcatatac aattcaaatt gaccttcacg caaatcgaat tccgttagcg
    22141 aggattgatc aaattgactg actaattctc tgatttcatt gatatccaaa attattcctc
    22201 ccagcgtttc atgcacagaa ccgcattatg tccgccaaac cctaatgaat tactcattgc
    22261 ataagtaaaa cttgcaggtt tgctttcgtt tttgacaact gtcaagtcaa tctcaggatc
    22321 tcgtttttct acattgatcg taggtgggat aaattgatgt tctaatgcca gcaaagttgc
    22381 caaggcttcg atcccgccgg ctgctcccaa taaatgtcca gtcatcgatt tcgtgctgct
    22441 gacgtaagta tctctgaaat tttcacctaa tgcgatttgg attgcttttg cttctgcctg
    22501 gtcattggct ggtgtacttg ttccgtgagc attgatatat ccgacttgtt ccggctgtac
    22561 accagcttct tccatcgcaa gcttcattgc tttagacgca ccagaaccat ctggagtagg
    22621 agctgtcata tggtaagcgt cacagtttgc accgtatcca atgatttctc catagatttt
    22681 ggcaccgcga gcttgtgcat gttctaaaga ttcaagaact agaacacctg ctccttcacc
    22741 catcacaaaa ccattccgat tttcgtcaaa cggaatcgaa ccacgattag gatcagttga
    22801 ttctgagagt gctgttaaag ctgcaaatcc agcaatcccc atctcacaaa tcgttgcttc
    22861 tgttcctcca gcaagaatga catcagaata gccatgtttg atgttacgga atgcttcccc
    22921 aattgaattc gttccgctag cacaagctgt tacgatagct gtacaaatac cttttgcacc
    22981 tacgcgcaaa gcaatatttc cagctgccat gttagcaatc gccattggta cgaacattgg
    23041 agaaactcgt tttgcacctt tttcatgtag acgaataact tggttttcga ttgttggaag
    23101 accaccgatc ccagatccga tgatgacgcc aaaccggtct tgattcattt tttctttatc
    23161 taaaccgctc atctccattg cttctaaagc agcgtagatt ccatagatcg aaaacatatc
    23221 cattcgtttg ctatcttttt taataaaata tttatcgtaa gggaaatctt tgacttcacc
    23281 cgctaaggta atccctgttt ctgaagcatc aaatttagtg atcggtgcga tgccattcgt
    23341 cccctctttt aagctattca aaaagctgtc tgcgtcattt ccaataggtg aagtgactcc
    23401 atagccagta attaccactc gattcatttg tttgctctcc ttttcttatc cgtgcatgac
    23461 caatccgcca tcgacattga tgacttgtcc ggtgatatat ggattttgtg ctaagaaaac
    23521 tgctgtttgt gcaatatctt ctacttgtcc gaaacgctgc atcggaattt gcttttccgc
    23581 ttgttccttg actttgtctg ctaaaacttc cgtcatatca gttgtgatga atcctggcgc
    23641 aatggcatta catgtaatgc ctctagttgc agcttctcgt gccacagatt ttgttaatcc
    23701 tactacgcca gctttactag ctgcataatt tgcctgacca atatttccaa tcaacccaga
    23761 tacactagag agattaatga tggccccttc tcgctgcttc atcatttttt tcaatacgtg
    23821 ctgcgtcata ttgaaggttc caatcaaatt gatatcaaga cattttttga aatcttctgc
    23881 atccattcgc atcacaagtt tgtcatttgt gattccggca ttgtttacta acacatggat
    23941 cgatccaagc ttttcttctg cttctttgat catttgtccc gctttctcat aatcagagat
    24001 gtcaccggaa actcctacgc attttacacc gaatgcttcg atttcttcta tcttttcttt
    24061 tggaatctct ccgcgaccat tcaaaatgat atttgctcca gcttttgcaa aagctaaggc
    24121 catcgctttg ccgattcctc ttgtactacc tgtgataaat acattttttc cttttacatc
    24181 cataacgttc ctccttatag tattgctatt gtttcatcaa atgtctgctt attctcaaca
    24241 cgcagaactg gtattgtttt atcgattttt ttcataaatc cgcttaatac ttttccgggg
    24301 ccgacttcga tgacttgttc gatgccgatt gtttttaact tatggatact ctcatagaaa
    24361 cgaacaggtt gcatgacttg ctgttcaagt agtcccgcga ttgtttcttt cttcattatt
    24421 tccgttgttg tgttgcttat gataggaaaa gaaggttcag agaattgaat ctgacttagg
    24481 tccttcgcca gtttttttgc tgccggttct aaaatagctg tatgaaaagg cccgctgaca
    24541 tttagaggga tcattctttt gaaccctttt tcttttaata gtaaaacagc ttcgtctact
    24601 gctttttctt cgccaccaat cacgatctgc tgaggtgtat tataatttgc aggagatacg
    24661 atgccgtact tgctagcttc atgacagctt tcctcgatcg tttcaatcgg cgcattcatc
    24721 actgcaacca ttttcccgct tccagctggg gcagcttctg tcatataagc gcctcttttt
    24781 gctactaacg caactgcttc agtgaaggat agcgcattac tagctactaa tgccgaatat
    24841 tctcctaaac tcagtcctaa agctgcatct gggatgattc catgctcctt caataagcga
    24901 taatacgcga tgctgactgt tagaatagct ggctgtgtat attgcgtctg atccaaacgt
    24961 tcattttctg taaagcaaag ttcagccata tcgtagccga gaatctcact tgcttcatca
    25021 aatgtctgct ttacaacggc ttcttcatat aaatcttttc ccataccttg atattgtgca
    25081 ccttggccac taaataagaa tgctgttttc atgtttttct ccaatatatc cagaatatat
    25141 gtttgattct tcctattcaa aaaggccgag acattcctat ttcaggaaat gtcccagccc
    25201 ccaacctaat tttaatgatt atgcagtttg tttttctacg taagtcacta aatcttgaac
    25261 cgttttgatt ccgtcttctg tttcgatttt cacgtcaaat tcatcttcaa tttcattgat
    25321 gatttggaat aaatccaagc tgtcagcttc taagtcttct tggatgtcag tagtcaattt
    25381 gatttcttca gtttcttttc ctaattcttc tgcgatgatt tcttgaattt tttcgaatac
    25441 cataataata tattcctccg atattgtttg ctttttattt ttataattta acagtctaca
    25501 actccatgag aatacttccc catgttaaac ctccgccata gcctgtaaat attactttct
    25561 gctgttttcc caaggtgatc tttccactag aaactgcttc atccaataag atagggatgc
    25621 ttgcagcaga tgtattccca tatttatcca tattggtcag gaacttttct tgcggcatct
    25681 ttacttttcg ggcaattttc tcaataattc tgaggtttgc ctgatgcaaa agcagataat
    25741 caactggttt gtctttcatg acttcttgga tattgtttgc aacgtctcgt acggcaaaat
    25801 caaaaatatc tctgccagtc atttgcaaat aatacgaacc ttctgaatct tctgaataga
    25861 acggattttt attttgctgg tagccagcgg tcaatgagct acttctggta ccgtcagctg
    25921 ctaatttttc tttcagcaat tttttctgtg gattcgcttc taagagaatc ccacctgctc
    25981 catcgccaaa caacacagca gtcgtacgat cagaccaatc gattagctta gatagtgttt
    26041 caccaccaat cactagtccc ttagttgagc cgcagcggat cattttttca gccatgctta
    26101 acgcataaac aaatcctgag cacgcagcac tgatatcaaa agcgatagca tgagttgctc
    26161 ccaatttccc ttgtacttgt gctgctacag atggcgtcgt gtaatcgggc gacattgttg
    26221 ctactaaaat aaaatccaaa tctttagggt ttagattttt ctgttttatc aatttttccg
    26281 ccactaaaat gcataaatca gaagtctcct gatttgttac acatcttcgt tcacgaatac
    26341 ccgtccttga cgaaatccat tcatcagagg tatccatcat cttggaaaga tcattattag
    26401 taataatctt ttcaggaagg acgctggctg ttgcagtaat cattccaaat tgttccatta
    26461 aatatcctca cttatagtct tgcaagaagt cgtgcaagtt tttcagtgca tccagcaagg
    26521 catgctgttc ctccgtagcc attccgtcta agatgttctt gaccatttca cgatgaaaat
    26581 gctgatggac ccgaaatagt aactttcctt ttttcgttag tcccaacttt acaacgcggc
    26641 ggtcatcttc actgcggata cgttcgacat agcctttttt tactaacttg ttaatagcag
    26701 tggtcaaggt cccaacagta attgatagtt ctttggctac ttctgaagtt gtttttttct
    26761 tgtacatccc gattgcctcg atcgtgtgca tctctgtgat tgacagatct ttgaattgag
    26821 attttttcag ctcagactct tcgattgtca aaatatcatt gaagacacta acaagataat
    26881 catttactgt ttttaaatct ggttccatgt tccaccgtcc actaattagt ttgattatca
    26941 aatcttttga tgttcaaact atatcttaac ttttttcaaa atgcaacctt aaaagatcat
    27001 ttttttacta tatgagcttt ttttaagatt ttactcattt aaaacaactg ataaatcagc
    27061 atttataatt tcttttatta gaaaaatcat ctatctcaca tattttgatt atcaaagtat
    27121 tttctgtgaa cagaactata gaattcctta aaacttgtcc attatttcaa aatttgttat
    27181 agtcttttta ttcaaagcgt aaaaggagat cctatgttaa aagaaaaaat gaaaactctc
    27241 cccatgctgc cgggtgtcta tcttatgaaa gaccgtgatg gtaaggtcat ctatgttggt
    27301 aaagcaaaac ggctcaaaaa tagagtaagc agctattttc atcagaataa acagcattca
    27361 aaaaaagtat tacgtatgat tcatcatata acagattttg atttcgtcgt agtagacacg
    27421 gaattagatg ctttattgct cgagtgtcaa ttgatccaac attaccgtcc tttttataac
    27481 cgacaaatga attatttcag taattataat tatgtacata ttaccaataa gggatttgta
    27541 ttaacagata ctcctacggc tcgaacatac gggccttttc gtctctataa aaaaatgccg
    27601 tctattttgc gtataatgga agaaacttat caaatgcctt ggttgtctga aattagtttg
    27661 ttagctttgc gagcccagct tcctgaccta caggaaatga cttttgaaca gaaaaaaaag
    27721 gaactccaag ggctgtttca aggccaaaat aaaaagctac taacttactt gaaaaaaaga
    27781 caacaacatt tcatctatca gttgaatttc gaaaaagccg gtatgcttca aaaagatatc
    27841 gaattagtta cttattttat tagacggatc caggaacaaa agcatttttt aagaacacca
    27901 tcgctcacct tcagcatgcc attagcagca gatgagagtc agaaaaagca ctacctcgtt
    27961 tgttatggtc aacttgcaga aacaatgatt gcctctgctg acgtttctcc tgacttctat
    28021 tatgaaaaga aagaagctca tttatctttg aaacgtcaat tgtcaaaaga agaaatcgat
    28081 cctgtacaaa tcttgatcag ttaccgcaaa aaattagaaa aagaacagtc tgaaattgaa
    28141 ctgttaaaca aaaaagaagc tgaaaagcaa ctaaattagt tgcttctcgg cttcttttag
    28201 gcatcttcaa cttcaaatgt caattattct aaaatttatt ggattaagtc atagatttcc
    28261 atcgctacga tatcgatatt atcaaattcg tattgctgat gagtatctgt ttcagttaat
    28321 tcaaatgtat cggtcgcttt atcataagtt actgtacact tctctacgcc ttctttttca
    28381 aaacggcgaa cttgcagttc attatctgct gactcgacca tagcctccag tcgttttatg
    28441 attgcgacta attgtgaatg tgtcatcgga ggtcctcctt ttcttatttc tgttatattg
    28501 tatcaaaagc atagcctact tgcattgatt ttctctaaaa aaactcgttt tttatgattt
    28561 tttttctggt atttttcctt caggccacca aagtttgatc aaagcttggg ttccgtcatt
    28621 ctcaaaacct ttatctgcca actcttcata tagtttcaat gcttgggtag ttgctggcaa
    28681 tacaaggtcc attttttctg cttcttctaa tgcaatcttt aaatctttga taaaatgttt
    28741 gacaaaaaaa ccaggtgtgt aatcttcttt taaaatacga ggagaataat tgcttaaaga
    28801 ccagttagct gcacttccac ctgaaagcgt ctcaatcact ttctttaaat caagtccgtt
    28861 tttttgtgca tagacaagca tttccgtcat tcctgtcatc gtacctgcaa tcatgatctg
    28921 attagccatt tttgtatgct gacctttccc agcagaacca tgcaatgtaa atgtcgtacc
    28981 aaattctttg aataaaggca gtacttgatc ataaacggct tcttctccac cgaccatgat
    29041 cgtcagtgta ccattctttg ctcctaagtc accaccggat accggtgcat ctaacgcatc
    29101 tgcgcctttt tcttttgctg tttttgcaat tttttctgcc aggcttggtg tacttgtagt
    29161 caagtcaact aaaatatgtc ctgagatatc tgcagaaaaa atccccgact gtccatagta
    29221 gatttcttct acatctttgg ggtaacctac cattgtaaag agaatattac tttgttctgc
    29281 taccgctttt ggtgtatctg cccagatagc tccttctgtt actaaaggat cagcttttgc
    29341 ttttgtccga ttatagacag ctacttcgtg tcctgcttct agtagatggc gtgcgacagc
    29401 acttcccatg acgcctgttc cgataaaacc aagtttcatt ttttcgacct cctaaaaatc
    29461 aaaaatcctt gcttctatgc tagtatacgc tagaaacaag gattttttca taaaaaatgt
    29521 ttgaatttta tcttttaaat gaatttaatt ttattttgat tcgttcaatc gagtcttctt
    29581 cttcttttct tcgcatctct tcttcatgat aaacaaccat gtcttgataa aattcgatca
    29641 tagcttcgcc gaaatgtgta gatgaaatat cataaagttt agcttctaaa acttttggat
    29701 ctttaggaac ttcactggaa atataatcaa taagtgctgg tgcaaagtct tcgtcggttt
    29761 caaaagtttt ccctaatgaa ggatgatcga acaatcggtt caaataagca tttccttcca
    29821 cgacacaagg tgttccggct gccatcgctt ctgtataagt aagcccttgt gtttctgaag
    29881 tagaagcact gacaaaataa tctgccgctt tataataaag tgctacatca ttgttgtcca
    29941 cttctcctgt gaactgcaca aattcttcta actgcagttc tgagatcaat tcctctaact
    30001 cttctacata aggtcctttt cctacgataa ctaatctggc gttaggataa gcggagatga
    30061 cctgaggaaa cccattaatc aaggcttgga tatttttttc ataagaaatc ctgcttaaag
    30121 aaagaatcat caaatggttt tcctgaagac ctagtgactc ccttaatttg ctgatatcct
    30181 gctgagtgat atcagaacgt ttgaattttt caatgtcgat accagtagga atcacgcgaa
    30241 gcggtgctat tacaccatag ctacgtaatg tatcaataac acgttcactt ggacaaacta
    30301 cacctgtcgt atgatttgca aataatctag acaagtattt tacatgggat tgcctgatca
    30361 cttttccttt accgatataa tgaaggtagt cttcatacat cgtatggtaa gtatggatca
    30421 gaggtatttt caacttctta gctaccatct tcccaagtat ccctgctccg aattctgtat
    30481 gggtatgaat aagatctagt tctagttctt tggcaaccaa ataagcatac cacatgcctc
    30541 taacaacaac tcttcgatcc ttgaaagaaa taaatggtac acttggcatt cgaatcacat
    30601 ctttttccaa cttatccgca ttaggatcgg tcgttgtaaa aatatagact tcatgcccat
    30661 gtctttctaa ttcttgtttt aatgttttga tcgatgtagc aactccgctg acttgcggaa
    30721 agtaggtatc ggtaaaaaag ccgattttca tgtttttcgc ctccaaacta gtccttgacc
    30781 tgatttactt tttttaattt gctagagact ttttcattca gtacactttc gtatacattt
    30841 ttaagttctt caccgatttt attgatactt cgctcttttg ctgtttgata accggcttgt
    30901 gtcgtactcg gtagttcttg attggtaatc tgttggatca gtcggataaa ttcttcgttt
    30961 gtcttcccca tatagcagtt cttgctatct tctaaccagc cattatacac tggaatatct
    31021 cgaacaagta cgttttgttt gcttgctaaa gcttctaaaa caacgatccc ttctgtctct
    31081 tcataagaag ggaaaaagaa tagatctgca cctgaatacg caccttctat gatctctcca
    31141 cgaatgtaac caggaaacag gacattttca ggatgatctt cttttacaat tttccggatg
    31201 ttccgaggaa tcgagtacat gggaacatga ccaaaccaaa taaatgtgta ttcgggcatc
    31261 ttcttagcaa tttctacgaa atcaatgatt cctttacgtt cgaaaaacaa accgacacaa
    31321 atgattactt tttgatccgg agacagtttg aaatattctc tgaacttttg ttctttcatt
    31381 ggatcagggc gatatttctc taagtcgatc ccattggaaa tagcttgaat cggcacacgg
    31441 atactatagc tttccaacaa ctgttttgaa taaggtgttg gcgtgatcag atagtccgct
    31501 ttttggtaca atcctaccag atattttttt acgataggag aaagctggtt cgatccgata
    31561 aaggaatttc ggaaatcttc ctctgtagaa tgcgcatgat agacaacttt ttttccttct
    31621 cttctggctt tattgaccat attatgacta ttgattccgt atgtattgat gtgcaggatg
    31681 tcataatctt ctttttcatc tagtgtataa gagataccga cttccgtcag agcacgtttt
    31741 tgatgatcaa gcgcacgccc gattccagat tttgcaagca tcttttcact ttcaaaatat
    31801 aataatacct tcaccttacg tcccccttca cttatatagt ataacatagc ctacaaactg
    31861 tcactcaaac ttaagcccta tttttatcta ttcttactga caaaaacgtt ttcttaaaaa
    31921 agaacaaaaa aactggcgta gctcttttac aagccacacc agcttttgat acgttgttta
    31981 ttttacatat tcatgaacaa gttcaacgac ttcttccatc gtatcgcatt ctttcaatgc
    32041 acgatcagca agttcagcca ttttcgctgt gtccaagcgt ttcattaaac tacgcgtttt
    32101 aagaatagat gtcgcactca ttgagaactc atctagaccc atacctacta gcaatggaac
    32161 agctgtttga tctccagcca tttctccaca catacctgcc cattttcctt cagcgtgtgc
    32221 tgcatcaatt acatttttga tcaaacgtaa aattgatggg ttatatggtt ggtacaagta
    32281 agaaacgcgt tcgttcatac ggtctgctgc cattgtgtat tgaatcaaat cgttcgtacc
    32341 tacagagaag aaatctactt cttttgcaaa cttgtctgct aagactgctg ctgctggaat
    32401 ctcgatcatg atgccgactt ggatcgtatc agatacttcc ttgccttcac tgatcaattt
    32461 ttgtttttct tcttcaaaga tcgcttttgc tgcgcggaat tctttgagtg tagctaccat
    32521 agggaacatg atacgtaagt taccatgaac agatgcacga agcaatgcac gcatttgtgt
    32581 acggaacatt tcgtcgcctt gttcagataa actgatacgc aatgcacgat atcctaagaa
    32641 tggattcatt tcatgaggta attgcagata tggtagttct ttatctccac cgatgtccat
    32701 tgtacgaaca actacaggtt ttccttccat accttctaat acagctttgt aagcttcata
    32761 ttggtcatct tctgttggga agtctggaga atccatatac aggaattcag tacggtacag
    32821 accaactgct tctccaccat tattatggac accaactaga tcttttggtg ttccgatgtt
    32881 tgcagctaat tcaaaatgct tgccgtcagc tgtgacagtt tctgcatttt ttaatttttc
    32941 ccattctgct ttttgtgctg cgtaatctgc acctgctttt tcaaattcag ctttttgttc
    33001 atctgtagga tcgatgatta catcgccttc gatcccgttg acagccaaaa tatcgccttc
    33061 tttgacttta gcagtaattt cttttgtacc aacgattgct ggaatttcta aagaacgtgc
    33121 catgatagca gaatgtgaag tacgtccacc aatgtctgtt acaaacgctt ttacaaaatt
    33181 gcgatctaat tgtgctgtat cacttggagt caagtcatgt gcaacaacga ccacttcttc
    33241 gttgatcatt gatggatttg gtaaagtaac gcctaataag tgcgctaaaa cacgtttagc
    33301 aacatcacga atatctgcag cacgctcttg catataagca ttatcttcca ttgcttcgaa
    33361 cataccgata tacatatccg ttacttcttt tagcgctgat tcagcattta cgctgttgtc
    33421 tttaatattt tgcttgattt gacctaccat ttctggatct gaaagaacca tcaaatgggc
    33481 atcaaatact tgcgcttctg cttcacctaa gctttgcgct gctttttcac gaatttgctg
    33541 caattcttca gtagatttag ctaatgcagc atccaaacga gcttcttcag ccgcagtatc
    33601 ttctactgtt gccttactga atgttaaatc cggttgaact agcaggtaag cttttgcaac
    33661 ggctacaccg tcactagcgg caatcccttt tagcatttca accattattc agataagcct
    33721 tctttcttca ttgtttcaac gattgctgcc attgcatcag cttcgtcagc accttctgca
    33781 gtaatagtaa catcagaacc ttgacctaca cctaaagaca taacgcccat gattgatttc
    33841 aagtttactg atttaccttt atactctaag tttacatcag agttaaattt gcttgctgtt
    33901 tgtactaata gtgtagctgg acgagcgtgg atcccagttt ctgctactac gtgaaattct
    33961 ttcttttcca tattaatcgg tctcctttaa gtgagtgttg tttttatgtg agggttttct
    34021 tttttagtaa ttcagagaat cctaacaaat gccacccttt catctgtaaa gattaacatt
    34081 ttttttcgtt agatacaaca gttttcattc aatttcagat aaaagtattc aaattattcc
    34141 ggattctttt tcatcctttt cttcacattt tataacaaac gtgtttattt tctttttctt
    34201 gtcgctttct cttctcctga cgtttatcat tctttccttt gaccctcttt gttttcatat
    34261 gaaacgaatt acttgcttcc ctttttattt ttagtataat aagagtcaaa ggtcaggaat
    34321 ggtcaataga gatttcacct tttctacctc ataaatctct taaaaaacta ggggttattc
    34381 ttttacctca tgaaaaacga gatgtaaaat atggatgtac ccatttttgt gaaaggacgt
    34441 gaatgattca tgctttgtca aaactgcggc aagaatgagg caacgattca cttgtatgcc
    34501 actgtcaatg gtcagcggac acaactcgat tactgtcaga gctgttatca aaaaatcaaa
    34561 aatcaacaaa atggaggtct gactaatatg gcccaaaatg atccatttgg atttggcagt
    34621 ttggacgatc ttttccgttc catgtctcgc caaatgcaac agcaaggaaa ttatgagcaa
    34681 acaccaccaa cacaatttgg cggaaacaac aacttcaatg gtggtcagcc acctcaaggc
    34741 aatacagaag gtttattggg agaatacgga atcaatatca cagaacaagc acgacaaggt
    34801 gatatcgatc ctgtgatcgg acgcgatgac gaaatcaaac gagtcattga aattttaaat
    34861 cgccgaacca agaacaatcc tgtcttgatt ggagaaccag gtgttggtaa aacagcagtt
    34921 gttgaaggtt tagcacaaaa aatcgtagat ggtgatgtac cgcaaaaatt aatggacaaa
    34981 gaagtcattc gtttagatgt cgtatcttta gtccaaggta ctggtatacg aggacaattt
    35041 gaagaacgta tgcaaaaatt gattgaagag atccgtcaag cagaaaatgt gatcctattc
    35101 atagatgaag ttcacgaaat cgtcggtgct ggttctgctg gtgatggaaa tatggatgct
    35161 ggtaatattt taaaaccagc tttagctcga ggagaattgc aaatggttgg tgctactacc
    35221 ttaaatgagt accgcatcat cgaaaaagac gctgctttag aacgtcgtat gcaacctgta
    35281 cgggtagatg aaccaactgt cgaagaaacg atcagtattt tgaaaggttt gcaaaaacgt
    35341 tatgaagact atcatcatgt aaaatatact gatgaggcca tcgaagcagc cgcaacttta
    35401 tccaatcgtt acgtccaaga tcgcttcttg ccggataaag ccatcgattt gctagatgaa
    35461 acaggttcta agaaaaactt gacgatccaa atcgttgatc ctaaaacaat cgagaaaaaa
    35521 ctgcaagaag cagaagaaca aaaagtttta gcttctcgag aagaagattt cgaaaaagca
    35581 gcttattatc gcgatcagat caataaactt caaaaaatga aagaacgtca gctcactgaa
    35641 gaggaaacac ctgtgattac cgaaaaagat atggaaaaaa tcgttgaaca acggacaggt
    35701 attccagtgg gtgaattaaa agaaaaagaa cagacacaac tgaaaaactt agcagatgac
    35761 ttgaaagctc atgtcatcgg acaagataac gctgtagatc gagtagcgaa agcgattcgc
    35821 cgaaaccgtg ttggattaaa caaacagaat cgtccaatcg gctccttctt atttgttgga
    35881 ccaactggtg taggtaaaac agagcttgcg aaacaacttg cctacgaact attcggttca
    35941 caagattcaa tgattcgttt cgatatgtct gaatatatgg aaaaacatag tgtctctaaa
    36001 ttgattggtt ctcctccagg ttatgtcgga tacgaagaag ctggtcaatt aacagaaaaa
    36061 gtacggagaa atccttacag tcttgtcttg ttagatgaag tagaaaaagc tcatccagat
    36121 gttttgcata tgttcttgca aatcttagat gatggtcgat tgacagatgc acaaggaaga
    36181 acggtcagct ttaaagatac gatcatcatt atgacaagta atgccggaac tggtaaagta
    36241 gaagcaaacg ttggttttgg tgcggcacgc gaaggtgtta ctcgttctgt attgaaccaa
    36301 ttaaacaact actttacacc agaatttctg aaccgttttg acgggatcat cgaattcagt
    36361 gcattatcaa aagaaaactt gatgacaatc gtcacactaa tgctagacga tgtcaaccaa
    36421 atgctggcag cacagcaact tcacatcgaa gttcctacga atgtcaaaga aaaattagtt
    36481 gacttaggct atgatccatc tatgggtgct cgtcctcttc gacgcacgat ccaagaacaa
    36541 atcgaagatg gtatcgctga attttatctt gatcaccctt ctatccatga gttgaaagca
    36601 aaactggata aagatggaaa aatcatcgtt acttctaaac cagaacgttt ggctaaagaa
    36661 tcagccgaag aaacggctga ataaatccag ttttatagcg tgagacggta tttctttgag
    36721 aaatgccgtc tcattctttt ttcagaaaag tcaaaatact gttcatttta taaaaaagaa
    36781 attataataa gagaaacaag caattaaaaa tacataaaag aggtgagcgc catgaaattg
    36841 gtaaatgtaa caaacagcca ttcccgtctt gtattaaacc agcttgaaaa cactgatgcc
    36901 catctagtca aggtctacac agccggaaat accactattg tctacactga agcccctgaa
    36961 cataatgaaa ttctgctgat caatgacaaa cgtaaaatcc agccgaaaga aatcgaagat
    37021 gctaaaaatt atttcttaga taaaatcaaa gacgccgttt accacgagga tgaaatcaaa
    37081 gtaatcgaat tggcaggcct tgttgaaatc tcgattccaa aacatgaatt aggcgctaca
    37141 ttagccgtgt aaacaaattt tgaacataat aattaaagag cgtgggacaa aagtaaagaa
    37201 cacttttgtc tcacgctcta aacacgtata aacggcggaa gcagaagcaa ctccttcgga
    37261 aataagctaa aattcacgaa aattgctttt caaatttttg tgaattccag cttatttctt
    37321 ggagttaaac gcttctgtct cagcctcttt ttctttctag ctttttgatt tttctctttt
    37381 cttttgtact tttctctttt cattttcagt tctacgcgtg tctttatttt taactttttc
    37441 ctctttatgg atgattgatc gtactttttg tgcaatttgc tcgtttttgt cccagtcttt
    37501 taaatctaat aatcgacata atcgtaacat ctcttctttt ttctgttttt ttctttcacg
    37561 tatttgccat tctgtttcca tgtgtcacac tctcctttct ttaatcatat gtacgcaaaa
    37621 taatttttat atttttttga ttagacattt ctatgatttt aatatagtaa aaagagccgt
    37681 cacggaaaat ccgtcacggc tctaaacaaa atatgagaga ctgtttttgt ttggctattc
    37741 ttattacttg atgcagggca gcttgtgtca agacttcttt acatcaaact ttttaatttt
    37801 acattaggat atttatccgc aaaccagcgt tctgcaaatt ggttctcgaa taagaacaat
    37861 ggttggtcga aacggtcacg agccaagata ttgcgacttg aactcatctt ctcatccaaa
    37921 tcttctggat cgatccagcg agcgattttg tgtcccattg gagacatgat tacttcggcg
    37981 ttatactcat ttaacatacg atattggaat acttcgaatt gtagctgacc aacagctcca
    38041 atgatatatt cttccgttag atatgtttta taaagctgaa tcgctccttc ttgaactaat
    38101 tgatagattc ctttgtggaa ggatttttgt ttcatgacat tttttgcagt cactttcata
    38161 aacagctctg gagtaaatga agggagttct tcataagcaa cttttagttt tccttcgaac
    38221 aatgtatccc caatctgata attgcctgta tcatatacac cgatgatatc gcctgctact
    38281 gctttttcga cgttttctct cgaatctgcc ataaattgtg tgacattgct taatttcagt
    38341 tttttatttg tacgttccaa ccagacatcc attcctcgtt cgaatgttcc agaacagatt
    38401 cggacaaaag cgattcggtc acgatgggct ggattcatat ttgcttggat cttgaatacg
    38461 aaacctgaaa attcttcctc ataaggactt accatctgat catcttgtgt tttgtgtcca
    38521 taaggagctg gtgccaattc aacgaaagtc tctaaaaaag tctgtacacc gaagttagtc
    38581 aatgcagaac caaaaaatac tggtgtctga tcgccacgag caattttttc ttgattaaat
    38641 gcatctccag cctctttgat tagttctact tcttctaaga cttgttcata taaggaattt
    38701 tcagttagag gatgattttc gggaagcagt ccatcttcat ccaattctac taatcgttca
    38761 tcctgataat tttccggacg ataaaactct acacgattat ggtagatatc ataaagacct
    38821 tctagccctt tacccatacc gattggccaa ttcatcggat aagattcgat atctaatagc
    38881 tcttctaatt cttctaacaa ttccaatggt tcacgaccat cacgatctag tttgttgata
    38941 aaagtaaata tcgggatgcc acgttttttt acaacttgga ataatttctt tgtctgtgct
    39001 tcgatccctt ttgcactatc gattaccatg accgcactat ccacggccat caaagtacga
    39061 taagtatctt cagaaaaatc ttcatgtcct ggtgtatcca agatatttac tcgtttccct
    39121 tgataatcaa actgcataac agaactagta acagaaatcc cacgttgttt ctcgatttcc
    39181 atccagtctg atttggcaaa attacctgtt tttttgcctt ttacggtccc ggcttgacgg
    39241 atagccccac caaacaatag taattgttct gtaatcgttg ttttcccagc atccggatgg
    39301 gaaatgatcg caaaagttct gcgattgtcc acttgttgct ttaattctgg attattcata
    39361 acctaactcc atttcttcca ttcaattatt tatattttct ctaggcacag attgtccgat
    39421 ctcttaaaga aagacaattt catatagggc tgtgacaaaa gccgtgtagc agtctctttt
    39481 tccgagtaaa cggcgttcta gagacagcct atcagcaata agcccaccca agccaaaaat
    39541 acgaaaagct gtttttgctt ggctgctctt attacttaat gcaggatagc tttttcacaa
    39601 cctttgtcaa ctactacaac tgaatcagta tagcatggat tcatcgaatg aaaaagccac
    39661 caaaccataa gagtcaaata tttttttgtc agctgactaa aagaaaagac gtgttgcttg
    39721 tttattcaag caacacgtct ttcataacca agtttccaat tatcttcttg gctcgtcatc
    39781 ttcgaattct tttctgaagt atcttcgttc ttcttcaggt tccgcttctg tttcttccgg
    39841 atcatgaaaa atgacacgga ttttaaggat tcgagaacct tccatttctt cagaaaccag
    39901 cgtaatatta tcgacatcaa acgataactt ttcgccttca tccggaatca tgcctaatgc
    39961 tgtaatcaaa taaccagcca tcgtatctac atcactcata tgcagatcac tgtcaaaagc
    40021 ttcattgaac tcatcaatca gcatacgtcc ttggatgata tattcgtgtt catcgatttt
    40081 ttcataaaga ttctcaactt catctgattc atcatcgatt tctccgacga tttcttctaa
    40141 aaggtcttct agtgttgcta acccaacgac gccgccatat tcatctaata aaatagccat
    40201 ctgattctgc gtctttttca tttcatataa cagatcatca ataaagaccg tttcaggaac
    40261 gaataaaggc tcctgcatga ttttcttgat atctaaatta tcaaaaccga atttatgggc
    40321 agcttttagt aaattctttg tatgaaggat tccgactact ttatctttgt cttcgttata
    40381 aacagggatt cttgaatagt tttcggataa aacttcattc acattttctt ccacagtgtc
    40441 gttgatatca atcataaatg catccgttcg tgggaccatg acttctcggg caactttcgt
    40501 atcaagagaa aaaacccctt ggagcatttc taattcttca ttattcaata cgccttcgtt
    40561 ttcaagcata taacgcattt catctctggt catttttgaa tcttcatcat caaaagtcat
    40621 cggcgtgata cgcgccaata aacttgtgga agcagaaagc agccagacga agggcttagc
    40681 tactacacca atcaagcgta tcagccctga agtgaacttt gcgacttctt ctgatttatt
    40741 taaagcaata cgtttagggt acaattctcc aaaaacaatc gatacatagg tcaaaatagc
    40801 cagcacaatg atatttgcaa tattttttgc cgctgtgccg ccacctaata ccggagctaa
    40861 ccgttccgat aaagtattag ctagtgaagc accagacaaa atgttgacca aagtgattcc
    40921 tacttggatt gtcgatagga aattatttgg attctgcaat atttttaaaa gcttttgaga
    40981 tttcacatct ccttcatcag ctttttgctc gattcgattc ttattgatcg aaacaacagc
    41041 aatttcggaa gctgctaaaa aagcattgat caatgtcaaa acgatcaata ataaaatctg
    41101 cgctaacagc gactgactct cagggtcagc attcataatc gcattctctc cttataaaat
    41161 taatttttag tttacgatag aaaaaaacgt ggcactatag ccacgttaaa atggtatcac
    41221 aaagcaattt tctttgcaat aaaaacaagt gtactaaaac tacctacgag gtcgtagcaa
    41281 ttttcttctt gttttcattt tgcttgtctt ctttgacgat tttgattata taggaaatga
    41341 tcgtcttgca gattgcgtaa aaaggaacac ctaagaagat accaagcaat ccggcaatat
    41401 ttccagctac tagaagaacg ataatgatcg ttaatggatg gattttcagt gatttcccaa
    41461 ttacattagg ataaatgata ttcccatcca actgttggac gattagcaca accacacaac
    41521 aaagtaatgc ttttaccgga tcattgaaga ctgttactag aaatgcaggt gctagtccta
    41581 gataaggtcc taaatatgga attaggtttg tcaatcccgc aatgacccca aataagaaag
    41641 cataatccac accaattacc atatatccta aaaatgtgaa ggtaccaacg aataaacatt
    41701 cgattgcttg tccgctaatg taattagcca atgttttatt cagttgcccc aataactcca
    41761 caatttggtt tcttcttttt tctgggaaaa actgttggat gtttggcact agtttttctc
    41821 cgtctttcaa catatagaac aagatgaatg gtgctgtaat gattactacg gttgccgaag
    41881 cgatcgttcc aacgatcgaa cctaaactat tggatactcc actaaggaat tgctgaatga
    41941 ttgtcccgta agaaatgtct aattgctcaa aatatttatt gatatctact tccttgaaca
    42001 aaggcaattc ggctacctct tttaaccagc tttctacctg tttgatgaaa tcaggcatat
    42061 tagatgccaa tgaggaaatt tgctgtacta gacttgggat gacactaagt aaaatccaaa
    42121 taattgctgc aactaaaagg agcagcacaa ttgcgacagc ccatattctt ttgatttttg
    42181 ctttcatcaa cagcttgaca ataggattca ataaatagta aagaaagcct gcaatcagta
    42241 caggtgcaaa taacgtggaa aaaaatgtcc cgatcggttc gaaaataaag tttatttctg
    42301 atgaaatcat gatcaatgca gctatgatca ataattcaag cgaccaaaac attagtttag
    42361 actgtctaag tttttcaaac aatagatttc ctcctttctt tcttactagt atatattata
    42421 ttcgttaaaa attcttttat tcttttttta tttttgaaaa cttgccatac tttttatttg
    42481 ttttattttc cagtatacta tacgaaagga aggtggctag accatgaaag ttgtacaaac
    42541 aaaagataca atgagcaata tttacttaga tgctgtacgg atcaggcatc aagtttttgt
    42601 agtcgaacag ggtatgcctt tgtctcgaga aatagacaaa gatgaagccc attgtatcca
    42661 cttcgtcctg tactcagata aaaaagaacc gcaaggaact gtacgtcttt tgcctttaga
    42721 aaacggaaaa atgaaattac agcggatggc gatccttttg aagatgctgg catgcctcat
    42781 atttatatga aaaagacttt ataaaaattt atttcgaaaa aagcaaaaaa cagcaagctt
    42841 ttgagaaaaa tctctcactg gcttactgtt cttttttgtg tttacttacg cgacaattgc
    42901 ttgtaacggt tataccaaat atcaatatag ccttccgaga acgggccatc tccattattg
    42961 atccagtcaa ccagaatctt cacattttct tttaggatga agtcaatatc gttcggataa
    43021 ttcatcattt tgctgtgcat ctcatattca tcgacatcca atagtcgttt ctcgccgtca
    43081 ggaaatacct tgatatccaa gtcatagtcg atatatttca aggcctcatc gtccagcaaa
    43141 aaaggtgaag ctagattaca ataataggaa accccctttt ctctgatcat tgctattaca
    43201 ttgaaccagt attttttatg aaaataaaca atcgctggtt cgcgcgttac ccatcggcgt
    43261 ccatcagatt cagtgactaa tgtgtgatca ttcacgccga tcatagagtg ttcgcttgtt
    43321 tttaatacca tggtatctcg ccacgttcga tgcaatttgc cgtcgtgctt gtaactttgg
    43381 atcgtcacaa actctccttc ttttggaact cccatagcaa cccttcctta agcagcattt
    43441 tgtgctttgt gtttgggtgt tcattacacc gacatcataa ttattatatc aataaaaatg
    43501 agctttgcct agaaaggatt cctattgttt cgatttttct ctaactgatc taccagtttc
    43561 atttggacct taggaaaaac gaccgaatca aatgcatcag gcttcaacca cttcgaagtt
    43621 ttgttctcgg taaattcttg ttctgctccc tcggttgccc gtccgtaaaa aaggagaaca
    43681 tgccatttca gatgactaaa aatatgagta acttctccta aatgtcgtgt ctgccaaaca
    43741 aagagttcct gcttttcaaa tgggagattt tgctcttctt ctgctactag gtcatcaaac
    43801 agatcgattt ctggctttgt ttcccattct tttttcaatc gctcatactc ctcttgtgtc
    43861 acttccatca ttggaaatgt ccacatattc gccagcaatt tttggctatc tctttcttca
    43921 aaataatacg ctccagaatg attttgaaga gccgcactga tataataaac gtcttttggc
    43981 tttgcttttt ttgtttttac aggaaaactc gtttggattc ctcgtttgtt agctaaacaa
    44041 aaggcttgga tcggacaagt ctcacacttt ggagaagttg gtgtacagat agctgaacct
    44101 agatccatca ttgcttggtt gaattcacct ggatgctttt catcaatgat tttccgcatc
    44161 gcttcatcaa agattttcct gctagaagct tttgcgatgt ctgcttcaat gcaaaacaat
    44221 ctgctgacta ctcgcatcac gttgccgtca accgcgggtt ctggaagtcc aaatgcgata
    44281 cttgcaatcg cgcctgtcgt atatggtcct attcccttca atgagctaat ttcttcaggc
    44341 gtttgcggca tttccccatc aaattctgac atgatctgtt tagcagcagc ttggatgttc
    44401 cgggctcttg agtaataacc aaggccctcc catgctttta aaagcttttc ttctggagca
    44461 tttgctagct cttcaattgt aggaaaccat tccatgaaac gataaaaata atcgatcact
    44521 gtgtctactc tggtttgctg aagcatgatc tcagagatcc agatacgata aggatcacga
    44581 ttatagcgcc aaggcaggtt tcttttttct tgttcatacc attgaatgaa ctgatcttga
    44641 aattcttttg tttctttatc tgtccatttt tcccattctt tattcacttt cctcttcctt
    44701 ttccgtaatg aatcgctcga tcatatccaa atcaaatcct ttttgatata agctttgctt
    44761 tacttttaga tttctttttt taggttcaaa ccttaaattt cttcgccata gtttatctcc
    44821 ttgtttgact aaataatcat attctatttc ttgatctgct tcttgcggaa gatcagccat
    44881 cgcttgttgg atcacttctt gagaaaaacc tttcgtcatc aatgtttgtt ggatttttct
    44941 caataattct ttttggcttt ttccgcgatt ttttccaaac gctttttcgg ctgtctgatg
    45001 tgcattttga acttggtctt caaatgtata ttggctgagc gcttgttcaa tgatttcagg
    45061 tttcaaacct ttttgctgca tctgctgcgc taatttcttt ggtcctttgt cacttaagcg
    45121 catattcgtg cgcacataac tttccccata gaccaaatca tcgacaaggt ccaattcttt
    45181 taagcgggct acgattttat ttcgatcttc ttgaaggatt tccttttctt ttaagtatgt
    45241 tcggatttct ttttccgatc gcaactgata acttaagtaa ttcattgcta ggtgaaaacc
    45301 gaaatcagct ccgctactct tcttgatctc ttggatagtt tcttcgtcta attcccgtcc
    45361 ttttaataat cgatagcgta ctaatacatc ttcagatact tgtaaagctt ctccttcaga
    45421 taaatctacg cgatagaaag ggcctttgcc tttactgatt ctcgtaacgg ttaacataaa
    45481 catcctcctc gaacatatat tctctatcat accacagaaa atccccgtaa tcaggtatta
    45541 tatgttagaa taaaccacag aaaaaagaaa aagggagata cttatggcag aaacaattgt
    45601 caaaactgga caaacggttc ctttgaaaat caagcgttta ggcataaatg gtgaaggaat
    45661 cggttatttc aaacggttga tcatatttgt tccatatgca ctaccaaaag aagaagtact
    45721 agtgaaaatc acgaaagcaa ctcctcgcta tgcagaagga gaattaataa aagtcaaaaa
    45781 aacaagcaaa gatcgcgtag tggcaccatg tcctgtttac tatgaatgcg gtggctgtca
    45841 attacaacac ttagcttatc aagctcaatt ggattttaaa aaggatctgc ttttgcaagc
    45901 attggaaaaa ttcaaaccag caggctttcg atcttatcaa cttttgccga cgatcgggat
    45961 ggatgagcct tggcattata gaaacaaagc acaatttcag ttacgaaaaa ataagcagac
    46021 tcaaaaaatt gaagctggtt tatacgaagc aaactctcat cagttagtcc ctataacaga
    46081 ctgcttggtt caagaacctc gaacacaaaa agtaatgaat actgttgttc aactattgaa
    46141 taaatttgat gcacccatct atgatgaacg gacaaacagt ggaatcttcc gcacaatcat
    46201 ggttagaatc ggtgttaaaa ctggggagtt acaagttgtt tttattactc gcagtaaaaa
    46261 attcccgcaa aaaaacgcaa tgatctgtga gatcaatgaa cgactgcccg aagttgtttc
    46321 aatcatgcaa aatgtccagg cacaaaaaac gtctctcgtg atgggggatg aaacgatcca
    46381 cttatgggga aaagaagcca ttgaagaaca aatcaatgaa gtgactttcg atctttctcc
    46441 tcgtgccttc tttcaactca atcctcagca gaccgaattg ctttatggcg aaggaatcaa
    46501 agcattagat gtccagccta atgaaacgat cgtggatgct tattgcggag ttgggacgat
    46561 cggcttaagt gtagcaaaac aagccaaaga agtacgggga atggataccg ttcctcaagc
    46621 aatcgctgat gcaaaaaaaa atgcggaaag attaggatat cacaacactc gatatgaagt
    46681 cgggacagca gagcaagttc tgcctaaatg gttgaatgaa ggttttcagc ctgatggtat
    46741 catcgtagat ccaccacgta cgggtttgga taacgcattg atccaatcat tattgaaata
    46801 ccctccaaaa aaacttgttt atatctcctg taatgtttct acacttgcca gagatctggt
    46861 taatttaagt aaaaagttca agattgaata tttacagtct gtcgacatgt ttcctcagac
    46921 tgcacgttgt gaagttgtcg tgaagatgac gagactcgct gactaaatga aagaggttgt
    46981 gaaaaagtct gccctgtatc aagtaataag aacagccaaa caaaaatagc tcttcatgtt
    47041 ttatgaatac cgtacttgtg accgaagagt taacaggagg acactattta tttaaaaaaa
    47101 gggttgtgac atagtttttg tcacagccct ttttgattat tattggatat tcacgtctat
    47161 cacttgataa aatgcatttg tcgtatcgct gatattccat actgctaaaa tcacattata
    47221 tccttttcga tcctgcggaa gtgtgatcgt ttggttgact gtttttggtg gaacagttgc
    47281 tttgtcgtct atttttgtga tcaattcaaa atcactaaat ttcaaaggtt gattaggatt
    47341 ccaagttggt ttggtaatgt agtaatccca agtacttgtc cgatgttgtg ctgttaaatt
    47401 ccaagtaata tttaatgttc ctggattaac catgttttta tgccatctgt tagatgtctg
    47461 ttcatctaaa ggctcaaagc cgctgactcc tgcactagca atttttccat caatgaatgt
    47521 atttttggta gcttcgatac tttgcggttc ccattgtgct cttccgacat tttgatttaa
    47581 gtttcctgct gctgatgttc ctaggtatgc acggcttcct ggagaagtaa tataaccatg
    47641 cgcaaaacct ggaatgctaa aaccgaacaa ccctaaacta actaacccaa taaacaataa
    47701 cgtactaatc tttttcatgt agaaacctcc ttcatttgat aaccacattg taaacgcttt
    47761 attgtattaa cacaaacatt atgcttttat aattttatgt tgaagtaaaa aaggtgaaaa
    47821 aagtaagatg cagattataa cagaagtaga cgagaaaagt acaaatagaa aacatgagaa
    47881 ccaaatcata tgatcttttt caaaaacatc attcatataa acaaatgttc aactttttaa
    47941 taacaacaat catcaatttt tttcttactt cacaaactaa atacaggtct tgctgtgagc
    48001 cttcatggta aactactatt atagaaagga gttagtaaaa tgaagatgat aaaagatatt
    48061 cgtttttggg tttgtgttat aggaattgtc atattaggct ttttatcagg gttgcttagc
    48121 ggaaatcccg gagaatatta ctattctttg caacttccac catttgcacc acctagctgg
    48181 atatttgggc cgatgtggac cttactgtat atattgatgg gtatttctct ttatttgtta
    48241 cttaatcaca acaacaaaaa acagcgaaac aatcttgtcg gtctttttgt gattcagttc
    48301 atttttaatt ttatatggtc tgctctattt tttaatttaa gaaatatttt cattgcagca
    48361 atcgatatta cacttttagt tattttcttg tcagtgctta tgtatcagtt gtggctacat
    48421 catcgcctgg caatgtggct catgattcct tattatttat gggtactatt cgcaactcta
    48481 ttgaattatt caatttattt tttaaactaa gcgctaaaaa gagacgtcct gtaatttaac
    48541 acatagaaaa agtgccacct aagttagatt ttttgtctaa tttaggtggc actctttatt
    48601 aaccaaacta tttttttcta gtacccactt gttgttccat actcattatt ctggtcataa
    48661 ccataaccat tttgttcctg tccagcgtaa ggatcataac tttgagtatc ttgacttggt
    48721 gtatagtact gacttgtatc ttctgtttgc tgataatagg tattcggatc ttggtactga
    48781 tcatatgtgt tttctgaata ggaagaataa tcggtgtacc cttcactatt cgatccatca
    48841 ccgtttgtcg aatcgccatc ttccgaatca tcaaagaaca tcattgaagc attctcattt
    48901 ttcaaattag gtaatgtatc gcttgttttt atatttaatt gtttcttcag ttcattttgg
    48961 atcgaaagaa gatcattttt tcctaaaatt tgatagtaga tatcattgat catttgtcct
    49021 tcgccttgca actggtcttg tttgattgtt tcaaacgcag gggtgtagtt cgtcgctaca
    49081 tcaagcatgt catcccaatc taagtccgtc gtcacattgt tctcaactgc ttttaaaatt
    49141 tttctataat tgcttacgcc atctaaactt aaaactttat tgacgatttt cgtcactact
    49201 tcccgctgac gacgttgtcg accgatatcg ccttctggat cttcgtgtcg cattcgagaa
    49261 taggctaaac ctttttctcc attcaatgtt tgttcaccta ctggtacatg gacaccatcc
    49321 aaagtaaagt caattttatt attcactttg atgccaccga ccgcattgat caaatcttcc
    49381 atgccatcca aattgatcgt tacataatga tcaataggaa tatcgagcag ttgctcaatt
    49441 gaatccatgg ccatttcgac accaccaaat gcataggcat gatttaattt atctaccgtt
    49501 ccataaccta cgatattcgt ataaatatct cggtccaaac tgatgatcgt tgattttttt
    49561 tgtttcggat taactgttac taccatcatc gtatcagaac gtccttgttc cgttcttcct
    49621 aatccaccgg tatctaaacc taatagcaaa accgaaaaag gttctttgtc atcaatgctt
    49681 actctctcag aacgtttgga ggatattcga ttagagtttt tagaaatttt gtttactgtt
    49741 tggctcgctt ctcccataaa tctgattccg tatgctgata atccggcaat cagcaaaaca
    49801 aataccccta acaaggttaa tagaactttt tgccatcgct tcataataca cctctgtttc
    49861 acattttcta aaatattgtc tcatataact gttcaatcag caaattcact ttttaatcat
    49921 aagaaaactt taaaaaacat ttttgtgtaa cgattactat tttttaaaat ttctcaaaat
    49981 tcttaactat cataccatac cgtgaaaaag atcgaaagac cgaatacacg cagttcgcaa
    50041 tcgttttatc tttttttacg tattttttcc agaaaaagcg aacaacaagg tattttccaa
    50101 ataatcgtgc agtaaaatga tcgcacatac ctttcccatc tttttaacag tctttgccta
    50161 ccgttcagat tatcattcga tttcacgaaa atttcttcct atgactggtc cttttgttaa
    50221 acccatcttc tattcgtttt atttccagct cactgcatgg ctcttaaaat gactcgaact
    50281 agtgatttca ttaccatcat cttttaatgg atagatatgc tcctcttcaa agtaaaacga
    50341 ttttctttta aagtaataga attgacatgg taatgagtat gtatactagt ggtttcacaa
    50401 atggtctttt attcaacatc caaaaactga aatttcttta aaatccatta ctaacgaaga
    50461 aaatgttcct tgcttgataa ttagttagga catgtcaagt gaacaaatgc atttacttag
    50521 ccaaaaaatc taaaaacttc ataaatagac aaaaagacac gactctctaa gaagtcgtgt
    50581 ctttattcaa caaattatta taatttagtc acttcagcag cttgaggtcc gcgtgcacct
    50641 tcaacaatcg tgaattctac ttcttgacct tcttctaggc tcttgaagcc gtctccttga
    50701 attgctgaga aatgaacaaa tacgtcatct ccgccgtcta ctgtaataaa cccaaatcct
    50761 ttttcattgt tgaaccattt aaccgttccg tgttccataa atgtatagcc tccaaaaaag
    50821 ttcttgcaag taaccttgca agaacattat acccaattta gaaaacgcta gcaagctttc
    50881 gctcttttat tttccaaatt atttttaaga ctgatatgac ggaggaaatg gttattttgc
    50941 tgcttcgaaa cgtttgttta cttcatccca attaataaca ttccagaatg ctgcaatata
    51001 atctggtcgg acatttttat atttcaaata ataagcatgt tcccaaacgt ccaaacctag
    51061 aattggtgtc tttccttcca tcaatggaga atcttgattt gcagtagagg taatagctaa
    51121 ttttccattt tccattacaa gccaagccca tccagaacca aatcgtccag cggctgcttt
    51181 tttgaattct tctttaaaag aagaaaaatc accaaaagct tcattaatcg cttcttttat
    51241 ttctcctgta ggttcgccac cagcatttgg tgccatgatt tcccagaaaa atgaatggtt
    51301 agcatgtccg ccaccattgt tacgtacagc tgtcttgata tctgttggaa tagcgtccat
    51361 atcagataat aattcttcta ttgttttttc gcctaattct gggtatttct caattgctgc
    51421 atttaaattc gtcacataag tattgtgatg cttatcatga tgcagatgca tcgtttcttc
    51481 gtcaatataa ggttccaatg cgtcatatgc ataaggtaga tctggtaatg tgtaagtcat
    51541 aaaaaaattc ctcctcaatg ttttctttat aatgtctaca ttacaaagag taacaaagag
    51601 aaggaaattc ttcaattgtt atgcctagtc gtctttttca ctttgaccta cttcttcttc
    51661 aaaaacctca gtttctttaa aaataaacaa cttgctgaaa atatagttcg ctaggataac
    51721 aataatttga gtgatgattt tcgccacata ctcattgctg ttcataacat tgatcaacaa
    51781 taccattgcc cccatgtcca atccgtaaga aaggatacgg tagaagaaga acttaccgca
    51841 ttcaataagt gcatgtgtaa aattcggtga ttttgaatgg aatacccaaa gtttgttcgt
    51901 tacaaaagca aacaatacag aacaaaacca cgaaatagaa ttggataagg ccatacttaa
    51961 atcaaaatat tggcgtgcga gagcaaaact aacaaaattt acaatcgtag ccaaaccacc
    52021 aaaaaagaga tagactagta tctcccaata tccttttgct tctaaatatc cttttaactg
    52081 tcgatactgc ttcatgagcc aacctacctt attaaaaatt cttatttatc atactctata
    52141 tttcaataac tttcatcttt atttaaaaaa attaattttt tttgatcctt tgcctttgtc
    52201 tctgcttgga tcgtcacatg tgtaatacca tattttaatt ttaataaaga aattatacga
    52261 ggatatatct tttcaatctc gcttagtagc tgatcttcaa aatcaatatg tccgtcgaaa
    52321 ataattatct tttcgctata ctgccaaata tggacatgat gaacattctt taccccatcg
    52381 attgcttcaa tttcttgttc cattttttca tagtcaatag caggtgcact ttgtatcaaa
    52441 atagcggcag cttttttgat caccattatc gcttctatca atatatatat cgaaatgatc
    52501 agtgtaataa ctggatcgat ccagtagata tcgaaaaaac ggatcaaaat tgctccgata
    52561 atcacactta ccgaagacag tgtatcactt aacaaatgca aataactaga ttttatattc
    52621 aaactttcat ctgcttcttg tcgtaataat aatactgaga acaaattcgc gagcaatcca
    52681 ataactgcta ctgttagcat caaatgacta tttatctttt ctggtgaagt gaaccgcttg
    52741 aatgcttcaa cggttaaaaa caaggataat atgatcaata tgaatgagtt gatgaaagca
    52801 ctcaatattt ctaaacgctg gtagccatac gtacgttttt tatttgcttc ttttcgggcc
    52861 attttttgtg ccccgtaaga aaaaataatt gccaatgtat cactaaggtt atggaaagca
    52921 tctgaaacaa gagacaaact ccctgacata atccctccaa taaattctga taaagtgatc
    52981 accgcattca aaaccactac tccaaatagt ttttttgatg atctctgtgg ctgtgtaggc
    53041 actattcctt cctccatttt atttatattg gatagtcttt agacaagaat aacatttttt
    53101 tgatatttca ccaatcattc agatgtttcc ctaaagcact tgtatttcac ttgatacttc
    53161 gctagaattg caagtgatga cactactaga tgaggagtac ttatgacaac aaaacaatta
    53221 attatcagtt cctttatacg attcaaagac ctaaaggagg cgagaaatgt cccattttgg
    53281 aaagccattg tctatctttt agttttgagt ataattatgg ctttgccaat ctcttttcaa
    53341 gtttttcaag tcttaaataa cattcaattg gatgggcaaa agattgctac tcgcattcct
    53401 gattttacaa tccaaaacgg gcagcttcaa accgaagaaa aagaaggatt catttaccaa
    53461 acaaactcca ttatcttcac atttgatcca gagggaaaac gaacagaaca ggacatctct
    53521 actgatttga tggggaattt tgttagtgtc gggatgttaa aaaacaaact tatcatagct
    53581 cttcctaata caggcacgac ttctgctttg ttgaacaata accaattaga acttccctac
    53641 accaatgaat ctttaaaaaa cttaacaggt aagcagctcc gttctttttt aagtgaagca
    53701 tctataccac tttggataaa agtcctgaca tttatatttt ctatttatcc tagttttctg
    53761 aatctgctga tcactttgct atttaccaac attgctgctt ttctctatgc acgctttcga
    53821 ctaacaaaag ctacttttct tgattgttta aaaacattga tctattcaat ttcattgcct
    53881 actataattg caacaatctt gatggtcttt cttccttctt ttgacactag cgccttcatt
    53941 gcttttgcag gaatctttat ttttgctcaa gctgtcaaag gatgggcaaa aatatcgatc
    54001 aattaagaaa taaagacaac tcgttatttc tacttaaata gaccctttta gaggttgtga
    54061 catttttttg tcacagcccc ttttttattt gaaaaatatt tctttctcaa aaaacaagca
    54121 aggcgcttca aagttcttct tgtatgcgct acacttagag taaagaaagg cgggaaatta
    54181 actatgtcag aaagaatcat ttatgcagat cgtccatttc gtgatgaatt tatcgaagaa
    54241 atccatacta tagcgcctgg ttatatcttt aaaacagaat tacaaccaga ggatctacaa
    54301 aatgtagaga tcagtttggg ctggaactca gactaccaag aaaaactatt agcctcttct
    54361 accttgaaat gggtccaatc tatctcagct ggtgttgatc agctacccct tgagactttt
    54421 tcaaagaaac atattttact ttcaaatggt agtggcatcc acagtgaatc gattacggag
    54481 catgtcttag gaatcatcct tgggtattct cgtggtcttt ttcaagcaca aaaggcacag
    54541 agtgacatga aatggttagg ttcatcggtc cattaccaat ctatccaagg gaaaaaaata
    54601 ctaatcattg gaactggcca tattggaaaa atgattgcaa aaaaatgcaa agcattagac
    54661 atgaactgct atggaatcaa tacatccggt catccagcag aaggaatgga aaagactttt
    54721 tctttaaatc aactaaagga agttcttcct gaagtagatt tcgtagtcaa tatcttacct
    54781 ctaacggagg aaacaactgg gctctttgat cagcatatgt ttgaacactt tgatccaaaa
    54841 tctgtattca tcaatgttgg ccgtggtgct tctgttaaga cacaagatct ggtgcaggct
    54901 ttaaataacc agcaactttc ctttgcagca ttagacgttt ttgaggaaga gcctcttcca
    54961 caagatcatc ctctttggaa aatggacaat gtactgatta cttcccacat tgcaggtttg
    55021 acacctgatt ttcaaaagaa attaatggct atttttttaa ccaatctgaa aagctacttt
    55081 gccacccatg agcttcaaac caatcaagta aaattgtctg caggatatta atagctgaaa
    55141 acaatatttt caaaagaaaa aagagaaggt gacaaaaatt gtgtcaccct ctcttttttt
    55201 attgtttttt aaagtgatag gttggctcct ccaggttggg atcataatca acaagtaaat
    55261 catatccttt aaagaaccat tcatctgctt cctcgataaa aaaaagtgtc ccatccattt
    55321 ccactttctt tacaggatgt tctggctgtt caactgacat tccaatcgaa aaaccatcat
    55381 gaacctctgt tttgccgtag acttttccaa aaaaacgtat tccgtaagtt tctggcagat
    55441 caatttctga gtcaaaccat ttttgtgctt ttggcgttac cgttaatttc atcccaacaa
    55501 cctactttct cttttctttc tattgattca tcttatcgtc tagaatatcc acatcgcctg
    55561 tctctccaat aaccagtaga ttatcatttt cacgaacata ttcatctgct gcaggagaag
    55621 cgataaccgt ttgatctact cttcgtatgg cgactactgt taagtcaaaa cgttgcctga
    55681 aatttaattc agctaatgtt ttgttgaaaa atttaggatt ggagactcgg atttctgcta
    55741 aagaatattt atctgacagc tctaagtagt ctagaatatt ctttgatact aagttatgtg
    55801 caatccgtat ccccatgtct cgctcagggt gtacgacacg atctgctccg atcttctcta
    55861 gtacacgggc atggtactca ttttgggctt ttgctaaaat attcggtacg cccatttcct
    55921 ttaccatcag agtaactaag atgctggctt ggatatcttc tccaatagcg acaattacat
    55981 gatcgaaatt tcttatacct aatgaacgaa gtgtcatctc atcctgtgca tttgccacaa
    56041 cagcatgggt ggctatattc atatattcat tgactcgatc ttcattactg tcaatagcca
    56101 gtacttcttg tccagcttcg atcagtgtac ggcagatgct tccgccaaat cgtccaaggc
    56161 ctacaattgc aaaattttgt ttcaccattt tcctccttta gtatcttctt ttctcagtat
    56221 aacaaagcct ttccaaaaac aaaagcaagc atcattattg taaaaaagcc aagaaaaata
    56281 atagttattt tctcagcttt tgattatcct ctttatcatc gtttttattt caccaaacca
    56341 tgtttaaacg catagatagc tgcttgagtt cggtcttcta cctctaactt agctaatata
    56401 ttcgacacat gcgttttgac tgttttcagt gtgataaaca attcatccgc gatttcctga
    56461 ttacttttcc cttcagaaat cagcatcaaa atttcatttt ctcgattcgt taattcttca
    56521 tgcaatactg gatcatttcg ccgactcatt ttttccatca tttttgttgt cacttcaggt
    56581 tctagtactc gctcgccgcg ttgcgtagcc cttatagcat cagctatttc atgtgctgta
    56641 gacgtcttca acaaatatcc tgctgctcct gcttcaattg caggataaac tttttcatca
    56701 tcaataaaac ttgtgacgat aatgattttg gcttttggcc aatcttttaa gattgctttt
    56761 gttgattcga tgccatccat ttcttccatg actagatcca ttaaaatgac atccggtcga
    56821 agtgccatcg ccttttcata gccttgacgt ccattttcag cttctccaat cacttcaatg
    56881 tcttcttgga tagataaata agaagagacg cctaagcgta ccatttcatg gtcatctact
    56941 aataaaactt ttatcatctt tatctatgcc tccttcatca aagggacctt ggtctccacg
    57001 ctcgtacctt gccctttaaa actaatgatt tttactgttc caccaatacc agctactcgt
    57061 tctttgatat tgttcaatcc ataacttccc gttttggttt cattcatatt aaatcctgtc
    57121 ccgtcgtcaa tgatacgtaa taaaagattg ttatctattt tatgcaaata gacttctaat
    57181 tcattagcct tagcatgtct taatgtgttt gataacaatt cttgaacgat tcggaacaag
    57241 tgatcctcaa tggaactagt taattttaca tcttctacct cccattttag cgaaatctgg
    57301 attttattct gcaattcctt caataactgc tcaatgcctt gttttaaact tttttcttct
    57361 aaattgactg gacgcagatg aagaagcagc gcacgcatct cagactggga agcgttgatg
    57421 atctctgcca ccattttcaa ctgcttacgg aacatttctg gtgtctcact tttttctgct
    57481 tgctccgtca atgccgacat catcatcatc gctgcaaata attgctgtga aactgaatca
    57541 tgaagctcac gagctaatcg atgcctttct aattttaaaa tctcttcttt ggtttgtcca
    57601 tctacatatt gaggacggct agtcataatc tgtaattcac tggatacttc gatcattttt
    57661 tcttttattt tagtaatttc catatcaata tctttgatgt acagatcatc cgacgcgctt
    57721 gggatcggct gatgcaaaag tttggattca taattgccag aagaaagtgc acgaagcttt
    57781 tcttcgattt tcccgtattt tgccttttga ataagtgata acaatagaaa tacgatcaat
    57841 cctacaccta atgaaattgc catcaaatgg aagataagcg gaacgtataa taaacgcgca
    57901 cgcagcaatt ctccccacca atgactttgg ttactggcat aaaaataagt gaaaagtgaa
    57961 aatagaataa taaggaaagc cgcaatcccc gaataaacag ctagcattgc tttggatatt
    58021 cttcccatca tattcgtatc acctcgacat ctccaaccaa agtattggta atgatcttca
    58081 atctgcgagg attttcatca taatcactgc tgcagatttt gatctgttcg ttttttaagg
    58141 aaaactgctc ttcttcaaac aagacatttc ctactaatgt cgcatgctct aaacgaatag
    58201 ccactcccag gggtactaag atccgtgttc tgcctatccc ttttcttaca atcacaatat
    58261 tatcatcttt tggcaaaagt gtattgccca aatcgatgat agtatcacca gaaatgatgg
    58321 caatgttgat atcatcccat tcatagacgt cgtttccaat acgttggtta ccaaataatt
    58381 gctgtctttt acgctcatta ttatgtgttt ttaattgctc tgtttggacc atcatgatct
    58441 gtttttttcg ccaaaaagca ttttttgtca aatctattcc tgaaatctct acacctttca
    58501 gcccgataaa taaaacggcc aatacaacca tcatccataa agcaggactg ttgactaagc
    58561 taaaaaaaat gaccaagctc cctaagatca attgaaaatt ttgaaattta gtacgaggat
    58621 atttgcgcat agctagatat atattgaaaa taccgaatat tactaaaagc aaaagtccag
    58681 tattgtttac gatttgccaa acagcaaata acagcagcag tgcttcgact accacaaaaa
    58741 aacgccaaga actattcatc gctttttctc cctcctgaca cattctcatt tcatcatacc
    58801 tatttttttg actgttggct ataggacttt agatgtaaga tcgttcagtg aaacctgcaa
    58861 tactcaaaga gaaaagatct agagtgcgtc atcagtaaga tgaactctct agatctttca
    58921 gtcagactac gcttgttcta ctttgacgat tttaactaac atatcgccac ctggtgtagc
    58981 aatggttact tcatcatttt tacgtttgcc aatcaatgct tgcgcgatag gagaatcatt
    59041 ggaaatcttt ccagaaaacg ggtctgcttc agcacttccg acaatcgtgt actcttcttc
    59101 atctccatca ggcaactcga tgaacgtaac tgttttaccg atcgatacct catctgcatc
    59161 tacgccgtca ttatcgatga tttgtgcaaa acggatcata ttttcaattg ttgtgattct
    59221 tccttcaaca aatgcttgtt catctttagc tgattcgtat tcggagtttt cggaaagatc
    59281 tccgaaaccg cgagcgattt tgattcgttc aatgatttct ccgcgtttaa ctgtttttag
    59341 ttcttctaat tctttttcta atttttcttt accttctaga gtcattggat aaactttctc
    59401 taccatgtgt aacgtctcct tttttcgata aaataccgca aaaggtaatc ttttgaggaa
    59461 tatagtccaa aaaagattac cacgcttttc ttaaaatgta aatgtttttc taaaaatgat
    59521 caatgactag cccctgtttt tctaagaatt atttacatat ttttgcacta gttccatatg
    59581 ttcttcatac gttttagcga aataaacatt tccagtgctc gtatctgcta cgaaataata
    59641 atagtcattt tctgctggtt ctaatactgc cttgattgcc tcttcgcttg gattgtcaaa
    59701 aggaccaggc ccatagccgg tattggtata caaattgtat ggtgaatcta cagctgtatc
    59761 ttcatacgtc accaattctt tatgttctcc taatgcatat aaaacagaga tatctgactg
    59821 aagtggcata tttgccttga ttcggttaaa gaagacttgt gcaatatttt tacgatcatt
    59881 ttcttttacg ccttcttttt caactagaga agccaaagtt aaaacttctt gtacagtcaa
    59941 atttttctgt ttgatagctg aataatagtt ttgcatgacc gtatttgttt tgttcaccat
    60001 ctcaatcaca agatccttaa gcgaagtact cgtgtagtaa tcataagtag ctgggaataa
    60061 gtagccttcc aaacggtatt ttgtgttctc agctttagag gcactttcca acaattctgg
    60121 gaatttttga tacaactcgt taaagaaagt ctcatcattt actaaatcta aaaattcttt
    60181 cttgtcttta ccagttacct tcgctacccg ctcagcaatc tgatcgatgt cgtagccttc
    60241 tggaatggca attttcccat ctgcaacttt tgttggctca gaagtgccgc cttcttgcag
    60301 ctgtttgcca atctcgtcaa gagtcatatt aggcgctaac tgataatagc cagcttgaaa
    60361 gccagttaaa tttttaaatt ttgtataata attgaaaaca atcccgcttt taataatatt
    60421 gtccttctcc aaaatctcac caatctgctt gttagaagag ccactcggga tctccacttg
    60481 gactaattgg tcatctgatt tatctaaagg cttcaacccg ctatctacat aacgatacac
    60541 ggtaaatcca agaaatccgc caataattaa taatgtaaga gcaatcacta aaatgatctt
    60601 cctcacaatc ttatcttctt ttttacgcag atttctccgc ttgtcgatta tatctgctgt
    60661 agctgcctta ctggcttctt ttttatcttt acttgggtgt tgtttgtttc ctgacaaaac
    60721 aactcctcct tactatcatc ttcgtaactc tgtttattat acatgaaatt tctactgccg
    60781 tgaattattt taccaaatat tatgtcaaaa ttgtaagtgg caaatactct ttttgaggat
    60841 tacaatgaaa aatagttaaa aatggataga aaggaattaa catgtcaaaa ggaaaacatc
    60901 gaaaaaaaca aaaaatttct atttcttttc ttgtaatttt attattgtct gtcagcttct
    60961 tttcaacagg catcattata ggaggtcaat taacaaaaat acctcattcc caaacttata
    61021 aagcggagcc aacacaactt acttccaatt taacgcccga tgagcagagg cttgctagca
    61081 aaattgatca attactacaa aaatcagatt atatcggttc gatttatgtt cgcaaaaata
    61141 atcgcgtact attagaaaaa ggatatggat atgcaaatct gcgcgtaaaa gaatcgaatt
    61201 caccttctat ttactatcaa atcggttcga ttcaaaaagc aatgactgct ttactgatac
    61261 taaaacaaat ccaagctggt aagttaactt ttgacacaaa gttagcagaa ttttatcctc
    61321 agattcctgg aagtcagcaa atcacaatca aaaatatgct ttatatgcgc tctggtcttc
    61381 atcgaactgc ttctcccgac aaacctatgt cggatagtga aatcattcag ttcgcccttc
    61441 atcatttgaa acttactgat tatgatacgt atcattatga accattgaat tatacattgc
    61501 ttacagggat attgatcaaa ctaacgaatc agccttatga aactcttcta aaaaaagaaa
    61561 tcattcgtcc actccatcta gaacatacag atttttatga caatgtaaaa aattcgcctc
    61621 aacatgctgt atcttaccag atgtctgcaa ctgacgatta ttcaaaagca ttgatcgagc
    61681 ctgaaaccga catccgaaat gaattaggta caggaaacat aagtatgtct gtttatgact
    61741 tgaacaaatt ctttaccagt gttctttctg gcggtatcat accaaaagaa ctacttttct
    61801 cattgtggca aaataatggt gataaacatc cttacagcgg cggtgtttac agtggaaacg
    61861 attatatcct tgctcagggg aatatcaatc gattccatgc agtagtggcg atgaaaaaag
    61921 acactaaaga tgcaatcgtc atggaaagta atatccaatc ggataagaac atcaaattgc
    61981 cggccactga tttaagaaat cagatttatg aactgatgga aggtgttcac ttatcaaatt
    62041 aaattttgac ataatattag ttgtcatctc tgtatagtca atttagttag tactctatct
    62101 gaaaagtaga aataaattaa caaatattat ctgtccttat tatttgttaa tttatttctt
    62161 atttt
//
LOCUS       DACECY010000008        59297 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.008, whole genome shotgun sequence.
ACCESSION   DACECY010000008 DACECY010000000
VERSION     DACECY010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.008"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(451..1761)
                     /locus_tag="IWO38_000542"
     CDS             complement(451..1761)
                     /locus_tag="IWO38_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ0312658.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="IWO38_000543"
     CDS             complement(1773..3191)
                     /locus_tag="IWO38_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0312659.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="IWO38_000544"
     CDS             complement(3431..4999)
                     /locus_tag="IWO38_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ0312660.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="IWO38_000545"
     CDS             complement(5003..5413)
                     /locus_tag="IWO38_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ0312661.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="IWO38_000546"
     CDS             complement(5410..6333)
                     /locus_tag="IWO38_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ0312662.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="IWO38_000547"
     CDS             complement(6330..7208)
                     /locus_tag="IWO38_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ0312663.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="IWO38_000548"
     CDS             complement(7195..8532)
                     /locus_tag="IWO38_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ0312664.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="IWO38_000549"
     CDS             complement(8535..9638)
                     /locus_tag="IWO38_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ0312665.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="IWO38_000550"
     CDS             complement(9712..11220)
                     /locus_tag="IWO38_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0312666.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="IWO38_000551"
     CDS             complement(11186..11878)
                     /locus_tag="IWO38_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0312667.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="IWO38_000552"
     CDS             complement(11900..12874)
                     /locus_tag="IWO38_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0312668.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="IWO38_000553"
     CDS             13174..14046
                     /locus_tag="IWO38_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0312669.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="IWO38_000554"
     CDS             complement(14504..16702)
                     /locus_tag="IWO38_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0312670.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="IWO38_000555"
     CDS             16934..17926
                     /locus_tag="IWO38_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0312671.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="IWO38_000556"
     CDS             17986..19407
                     /locus_tag="IWO38_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ0312672.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19400..20899
                     /locus_tag="IWO38_000557"
     CDS             19400..20899
                     /locus_tag="IWO38_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ0312673.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20938..22332
                     /gene="uxaC"
                     /locus_tag="IWO38_000558"
     CDS             20938..22332
                     /gene="uxaC"
                     /locus_tag="IWO38_000558"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ0312674.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22491..23174
                     /locus_tag="IWO38_000559"
     CDS             22491..23174
                     /locus_tag="IWO38_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ0312675.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23176..24012
                     /locus_tag="IWO38_000560"
     CDS             23176..24012
                     /locus_tag="IWO38_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0312676.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24023..25777
                     /locus_tag="IWO38_000561"
     CDS             24023..25777
                     /locus_tag="IWO38_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0312677.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26053..26394)
                     /locus_tag="IWO38_000562"
     CDS             complement(26053..26394)
                     /locus_tag="IWO38_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ0312678.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26462..28633)
                     /locus_tag="IWO38_000563"
     CDS             complement(26462..28633)
                     /locus_tag="IWO38_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ0312679.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28844..29701
                     /locus_tag="IWO38_000564"
     CDS             28844..29701
                     /locus_tag="IWO38_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0312680.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29772..30629)
                     /locus_tag="IWO38_000565"
     CDS             complement(29772..30629)
                     /locus_tag="IWO38_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0312681.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30614..33316)
                     /locus_tag="IWO38_000566"
     CDS             complement(30614..33316)
                     /locus_tag="IWO38_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0312682.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33329..34618)
                     /locus_tag="IWO38_000567"
     CDS             complement(33329..34618)
                     /locus_tag="IWO38_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ0312683.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34768..35814
                     /locus_tag="IWO38_000568"
     CDS             34768..35814
                     /locus_tag="IWO38_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0312684.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35816..37969
                     /locus_tag="IWO38_000569"
     CDS             35816..37969
                     /locus_tag="IWO38_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0312685.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(38442..39893)
                     /locus_tag="IWO38_000570"
     CDS             complement(38442..39893)
                     /locus_tag="IWO38_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0312686.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(39890..41614)
                     /locus_tag="IWO38_000571"
     CDS             complement(39890..41614)
                     /locus_tag="IWO38_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0312687.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(41607..42221)
                     /locus_tag="IWO38_000572"
     CDS             complement(41607..42221)
                     /locus_tag="IWO38_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0312688.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(42566..44023)
                     /locus_tag="IWO38_000573"
     CDS             complement(42566..44023)
                     /locus_tag="IWO38_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0312689.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(44064..44984)
                     /locus_tag="IWO38_000574"
     CDS             complement(44064..44984)
                     /locus_tag="IWO38_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0312690.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(44996..45943)
                     /locus_tag="IWO38_000575"
     CDS             complement(44996..45943)
                     /locus_tag="IWO38_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0312691.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(46422..47141)
                     /locus_tag="IWO38_000576"
     CDS             complement(46422..47141)
                     /locus_tag="IWO38_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ0312692.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(47190..48836)
                     /locus_tag="IWO38_000577"
     CDS             complement(47190..48836)
                     /locus_tag="IWO38_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0312693.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            49015..49605
                     /locus_tag="IWO38_000578"
     CDS             49015..49605
                     /locus_tag="IWO38_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0312694.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            49697..51202
                     /locus_tag="IWO38_000579"
     CDS             49697..51202
                     /locus_tag="IWO38_000579"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ0312695.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(51286..52638)
                     /gene="celB"
                     /locus_tag="IWO38_000580"
     CDS             complement(51286..52638)
                     /gene="celB"
                     /locus_tag="IWO38_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0312696.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(52638..53156)
                     /locus_tag="IWO38_000581"
     CDS             complement(52638..53156)
                     /locus_tag="IWO38_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312697.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(53172..53498)
                     /locus_tag="IWO38_000582"
     CDS             complement(53172..53498)
                     /locus_tag="IWO38_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ0312698.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(53511..55490)
                     /locus_tag="IWO38_000583"
     CDS             complement(53511..55490)
                     /locus_tag="IWO38_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0312699.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(55511..55825)
                     /locus_tag="IWO38_000584"
     CDS             complement(55511..55825)
                     /locus_tag="IWO38_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ0312700.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            55993..56286
                     /locus_tag="IWO38_000585"
     CDS             55993..56286
                     /locus_tag="IWO38_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ0312701.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(56364..57476)
                     /locus_tag="IWO38_000586"
     CDS             complement(56364..57476)
                     /locus_tag="IWO38_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0312702.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            57629..57853
                     /locus_tag="IWO38_000587"
     CDS             57629..57853
                     /locus_tag="IWO38_000587"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312703.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            57863..58042
                     /locus_tag="IWO38_000588"
     CDS             57863..58042
                     /locus_tag="IWO38_000588"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312704.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            58236..58406
                     /locus_tag="IWO38_000589"
     CDS             58236..58406
                     /locus_tag="IWO38_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0312705.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(58830..>59297)
                     /locus_tag="IWO38_000590"
     CDS             complement(58830..>59297)
                     /locus_tag="IWO38_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0312706.1"
                     /translation="VELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQS
                     GLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRK
                     VADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT        17760 a        12086 c        10360 g        19091 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgatct gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaatttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tgtcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcctccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg
    19441 agacagtgtg gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca
    19501 gcaaacgatc accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt
    19561 gaaagctgga gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt
    19621 aactgccggt caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga
    19681 ttatcgatat gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca
    19741 aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac
    19801 cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc
    19861 agatctggga cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct
    19921 tggaaaagat catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc
    19981 cggaggagtt ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa
    20041 aattttaggt gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga
    20101 tgagatcgaa caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca
    20161 gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg
    20221 tctatcggga ataactgcta atcctttact tggtgcattt tctgactact tgattgctca
    20281 aggaggcagc acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat
    20341 ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa
    20401 acagtatttc ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc
    20461 aggtgggatc actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc
    20521 tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt
    20581 gcaagctccc gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt
    20641 ggtcttgttt accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt
    20701 atctacgaat acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt
    20761 cttattagaa caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt
    20821 agctagtgga gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa
    20881 aaacggagta acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg
    20941 tttttatctg atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac
    21001 gcaaaaaaca tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa
    21061 aacaacaatt tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc
    21121 ttgatgcgag cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa
    21181 aaatttttag cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg
    21241 tcacatcttg aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca
    21301 gagaaaattt ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa
    21361 atcgcaaaaa aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg
    21421 cactatcatc agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt
    21481 ccagataaag cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa
    21541 gaagcagctg accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg
    21601 atagaatact tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc
    21661 tataaagaag cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa
    21721 ctattatctt cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac
    21781 ttctatcatt ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat
    21841 acacaaatgt tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct
    21901 ttgactattc ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag
    21961 acgatccttt attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt
    22021 ttccaaaaag aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact
    22081 cgagctggta tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt
    22141 gtcggcatgc tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt
    22201 cgtgttcttt gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa
    22261 caacttttag gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc
    22321 ttttttgcct aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac
    22381 ggaatccgtt tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct
    22441 cttgctgttc tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta
    22501 tccatattgc cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag
    22561 gttggggaga aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa
    22621 agaatggacg cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac
    22681 agtttcagcc gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag
    22741 aagaaagagg tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat
    22801 gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact
    22861 tagaaaacgg aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat
    22921 ttgctgagaa acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc
    22981 agaccttctc aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta
    23041 aaaattaccc agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag
    23101 ttgcccgaat aattgttgaa ttgataaaag aatcccgtct gcctcttgct aattatttac
    23161 aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt
    23221 cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt
    23281 gcaccacaaa aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct
    23341 gcctattttg gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat
    23401 atcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag
    23461 caaattgctc tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc
    23521 tctgtcggtt acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct
    23581 atcattgaat tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg
    23641 atcaatcatc caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca
    23701 ccaaatttaa aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat
    23761 caggaaaaag tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat
    23821 ctcaaagact atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt
    23881 ttagatttcc gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact
    23941 cttgaagcta ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac
    24001 caagattgtt aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca
    24061 tgcaaatgct tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat
    24121 gtcaggactc ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc
    24181 caatgtaatc aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa
    24241 cgcttttttg atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa
    24301 ataccatagc acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa
    24361 aatccaagaa ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa
    24421 gatcatccgt gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt
    24481 aaatatcgct gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt
    24541 tttcatgttt ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa
    24601 aaggatcaga cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt
    24661 gatcgaagtg atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac
    24721 aactcgctta aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct
    24781 gcaatctgtt ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt
    24841 gattttcact gggatattag ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta
    24901 tcagacgtat ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac
    24961 gatttcaaaa ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt
    25021 agaaacttat cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga
    25081 tgtttctttt ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt
    25141 gaaagctgga gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct
    25201 taacttattg attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc
    25261 tttggatgaa ctggatatgc gaagtgtgcg aaattattta gccgttgttc ctcaaaccac
    25321 tttgttgttc tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa
    25381 agaacgttta gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc
    25441 tgatggtctg gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca
    25501 acgtatctcc attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc
    25561 aacctcagct ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac
    25621 agaaacaccc actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa
    25681 aatcgttgtg atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc
    25741 tcaaaaaggc tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag
    25801 acttttgtcg tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac
    25861 aagtcacaat agtcatcaaa catgagaagc tgttttttga aattgttcct tgtaactcag
    25921 agttaaacgg taggctcacg acctcttggt aaatggtgtt caagagacag cctatcggca
    25981 ataagaagca attttgtcat cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt
    26041 cataaaatag ttttattcgc tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa
    26101 gtcattgatg attgtactga agccttgctc tttttgcatc aagtcattga ttacttcttc
    26161 tgattggatt ttcacagcca tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg
    26221 tccactcatt tgtttttctt gtaattcgat ttgcatcgct tggaagtctt tgaacagttt
    26281 gtgtgcttct tcgtttgctt gtactttctc gtacgcttct ttcaacgctt tgaattctgg
    26341 taattcacgg atctcacgtt cgatttgatt cgcgctatcg tagatgtttg acatgttatg
    26401 ccctcttttc ttttagtagt tactctctat tgtaacatgg ggttattcgt tttcaaaggg
    26461 gctactctcc ttgatatttc cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc
    26521 cttctttcac catatcgccg aagtttttca gcccttcttt tgcttgttca ccatattgat
    26581 tcaagttatc ttgccaattt ccagactctc cattagaatt tcctgtgttt ccaacttgat
    26641 ctgcagctac cacttgtcct cctgtggcat aggcatctgc tacactaaat cctgtttttg
    26701 aagtatacgg aaggatgcct tctgccgctg atttgaagac tttcccgaca acatttccac
    26761 ttgtcccttc caaatagtgc agcttgctgg attcttcgta acccatccat gtcgaaatca
    26821 ctacgtctgg cgtgtaaccg atcatccatt ggtcatttgc ttttgtggca tcgaaattag
    26881 tttctgtggt tcctgttttc cctgctatcg tataacctgc aggattcgca gcagccgctg
    26941 ttccatttga aaatgtacca agaagcatgc tggtcatttc atcggctgtt tcctttgata
    27001 tgacctgttt tggttttttg tttgtgttat cgacgatcac cgctccagtc gaatcaacga
    27061 ttttcgtgat aagatgcggc gtatacatct gaccatcatt ggcaaatgca ctgtaagcac
    27121 ccgccatgat caatggagat actccttttt ccagtccacc aagggctaag ccataatact
    27181 tatcagattt tgctaaaggc aggccgaatt cttctgcttt atcatatcct ttttgtaaac
    27241 cgatctcatg caaaagccat acagtaggca aattcaaact ttgtgcaaca gcttgataca
    27301 tcggtacttc tccttggtat gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt
    27361 cgtctttcaa gatactggaa ggcttgtatc cagcttccag tgctggtgta tatacgctta
    27421 atggtttgat cgttgatcct ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa
    27481 agacatgatc tcctcttcgt cctactagtg cttgtacacc acctgtattc ggatctaaag
    27541 ctactgaagc agattggacc atggcaccat ctgaagcatt tgctgggaat aatgcactgt
    27601 tttgataagt agcttctaat tgcttttgat aatcctgatc taaagctgta tagattttat
    27661 agcctttatt catgacatct tcttcatcaa gaccatactc gttaactgct tcatcgatca
    27721 ctgcatcaaa ataataagga taacgataac cagaatctga attatcgtat gtgtcattaa
    27781 gatacgcgct gatatctgtc tggctttctg cttgttcttg ttcttttgtg atttttccgt
    27841 tttcttccat tacacttagt actgtgtttt tccgatcatt cgcatttttt gggtgatcga
    27901 tcggattata aataccagga cctttcagca ttcctgccaa agtagcagcc tcagacagcg
    27961 taacattcgt tgcatctact ccaaaatact ttctggaagc gtcttggaca ccccataccc
    28021 cgttgccaaa ataggcattg ttaagataca tggtcaagat ttcttctttg ctgtacttct
    28081 tttcgatctc gattgccaaa aataattcct tcgcttttcg atcaaacgtt tggtctaatg
    28141 tcagatacgc atttttggca agctgctgag tgatcgtact tcctccgccc cctgatgttc
    28201 cgccagacaa gaccattcgg actgctgcac gggcaatccc tttgatatca aacccgtgat
    28261 gctcgtaaaa attcctatct tctgtcgaga ttactgcatc ttggatatag ggagaaatcg
    28321 cgtttaattc tacatatgtt cctttttggg cgtacagtgt gccagcttct tcgtttttct
    28381 tatcataaat gactgtcgat tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt
    28441 ttgctaaaat aaagaggtag atacttgtca ctaagacaca gatcaaacca atcagcaaaa
    28501 tcaatttgtt cacatgatat tttttccaga tgcgctttcg ccatcgatgg aattgtccga
    28561 gatatggtct gatccaacgc caaaaactga tcaaagcagc ttttatcttt tctaaaatgc
    28621 tttgaaaatc catttgcttc ttctcctgtc tatttgattc aatcgtctta ttttaccata
    28681 gtgaaagagt gttttcttcc tgcttaagtt gtagttcaat atgttttttt acgttagtgc
    28741 aagtagagta acataagaat gaacagaaaa atgtaagttt tttcttaaat atgttttaaa
    28801 aatggtacaa ttagtctgcg attgcactga aaggagtaca cttgtgaaaa tcacgatcac
    28861 tttacctatg aaccatcctg aaacaacaat tcgcgacttg cttgaaaagg aatggttagt
    28921 tcctagaaag gttcgacact ttttgcgtat acgtaaaaac gtttggatca atcaacaacc
    28981 tgctttgttc catcatgaag taaagggcaa tgaccagatc acattgttat tcgaagaaac
    29041 agattataat tatcaagaag ttcaattagg agatgcgaag cacatccgtg ttttatatga
    29101 agatgagcat ttgattatag tggataagcc tgcaggaatg aagactcatc caaatgaacc
    29161 gacagaaaac agtaccttac tcaatcattt agctgcttat ctcgacacta aacagcaacg
    29221 accttacgtt gtccaccgct tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa
    29281 tccatttgtc ttgccgatac ttggaagaat gttggaacaa aaaatgatct atcggagata
    29341 tcaagccatc gtatggggag aaattgccaa agatatgacc atcaccaata aaattggacg
    29401 agatcgccat gatcggagaa aaagggtcgt agatgataaa aatggtcaga gcgccttgac
    29461 acatgtcact gtcaacaaag tactttctga acaaacacaa gtctattgtg ttttggaaac
    29521 cggacgtaca caccagatca gagttcattt agctcataag ggacatccga tcgttggaga
    29581 ccctctctac caaaatagaa cagctgaacg actgatgctc catgcattag aactgcacat
    29641 gatccatcca tttactcaag aaaaaatagt cggtaaagct ttacctggat tatgggaata
    29701 gaaataaaaa agaacagagt cttatcagca gataatgata agactctgtt ctttttgtgt
    29761 aatttgtctg cttacttttg atgttcgtgt tctgctaaca gcaaactacc gataatccca
    29821 ctttcattcg gcagtcccca acgaacgata tattcactta cttgaggtgt ttccatataa
    29881 ccacccatca attcaacaaa ttgctgacgg attttttgaa ggagatgatc ctgattcatt
    29941 accccaccgc ctaaaataat cttttcaggt gctaacgtca acgtatagtt gatcagtgct
    30001 tgagcaatat aataagcctg aatagcccaa acaggatgat cttgcggcag atcttgccct
    30061 ttgatccctg tgcgtgcctc aatagacggt cctgctgcta atccttctaa acaatcttta
    30121 tgataaggac aagtaccttc ataatggtca tctggatggc gtttcaccca aatatgtccc
    30181 atttcaggat gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt
    30241 cctgttccca cggtaaggta aatacaacta ttttttcctt gtgctgctcc ttgcttcaat
    30301 tccccatatg cagcagcatt gacatccgtt gtccaagcag caggaatatt gtagcgttcc
    30361 ttgattgtgc caagaaaatc gaattgtgcc cacccttttt tgggagttgc caaaacgtat
    30421 ccgtaattat cttttgtttt gtcgatacca atcgggccga atgagccaat cccgattccc
    30481 ttcaattcat aacgatcaaa aaattcaaat acctgatcca atgtttcttc tggtgttgtg
    30541 gtagggatac ttaatttttc taaaatttct ccaccatctg aaaccgcaca cacaaatttt
    30601 gttccgcctg cttctattcc gccatacatt cgttttcctc ctcgaatccg attgtccgaa
    30661 tctcataagg ttggattatt gggacaaccg cttgcttcgt tggttcttca agcagattca
    30721 atatgacagt ttttccattg tctttagtta tttccaattt ttctaaatga ctagacatat
    30781 tgaaacctct tactactact ttatcactaa acttgctgcg tttcaaagct gtaattgcaa
    30841 acgtttcact ttttatcgtt aaatattgtt gttttgaaat caatgttcct tcatgatgtt
    30901 tgatttgttg agtggaaaaa ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt
    30961 tttcttctgg tccatgtagt tcaatgctgt aattgaaccg atgctcacct agacactgtg
    31021 cttctggtgt tggaaaatat ccccaatcgc ctagttctcc cacactgcga agtagcgtca
    31081 ctgcgatttg tccgtcttct gttacttcat attcattcaa accaaagttt ccgactgtta
    31141 caccgtactc ttcatcatga atattgacaa atgcttgctg atgttgcgga ttcgtggggt
    31201 tttcccaact tttagacaca tggtttgggc gcttcaccac ttcaaaaata ctatctgctt
    31261 cgtgatgttc cacatgtaac tttgtaggaa acaagacacg taagcgatga tctttcatct
    31321 gattgtcgag agtcgtttca aagtcgattt ttttactatc ttttctgatt gtcatgattg
    31381 tttttatttg aaggattcgt ttttctttgg atcgttctgc ttttcgataa cgaaattcca
    31441 caaccatctg ctgttctttt tctaaacgct cgtctgcaga aacagggatt tccatttcct
    31501 gtagaatact tatttttgct atttccggtg tatctactaa tacttttact tcagagtttt
    31561 ctttgttact ggataggatt ggctgatcac agaaaggttg tttaaagata tactcatttc
    31621 ctatatcgcc gatatcttca aaaatatgca gattattcaa ctttcgtttg tttttgcgat
    31681 cttccattgt aatggtacca tttttttcaa tcgtcaaatg tagagggccg ttttcgatga
    31741 ttcgtccact ctgatgaatc attgtttcct tttcttctgc tttcgcttcc ccttctacaa
    31801 gcgcgaatgt ttcccaagaa aatgctggca tgttttctaa tggtaaagtc actttgatca
    31861 ttcttgccat atatggcaca cggaaacggt ctttcggcaa atcataacca aattggacag
    31921 tttcttcaga taaaatagct ggtatagtgg ccccactttt tgtttctaca tgatacttac
    31981 gtttaggcaa attttctaac tcttgatata gttgttcagg tattccttca gcgaatcttt
    32041 ttctttctaa ttcgatggtt atttctgctt caccagtttt atccattcct gcagtattta
    32101 caatgacaaa tggaaagctg tcttttggga atccggtcgt atcgattgct gcggtcagct
    32161 gttctaagga atcttgtgca agatactttc caacttcatc tgctttttca aaacgaggta
    32221 tcatttcccg atggacagaa tcgactgaac aaccgcaaat actgtcgtga ggatgatttt
    32281 gcattaatgt cttccaagca taatctaatt gatcatgggg atagtttcca ctaacttcat
    32341 aagccatcgt cgcaagtggt tctgtaatat tttctagctg tcgctggacc ttggtattcc
    32401 attgttttaa gtaaacccga gcagatgctg tattggctaa agtataccag ccatccgttt
    32461 cttgacttgt caattctcct tctactgagc ctaagtcttc tggcacatct ttttgaactg
    32521 cttccagata gtcagtaaaa ttagaatgaa taaactcgta atctggaaac agttcatttg
    32581 cgagatggat tgcttttgat atatcttttt gtaccggttg atgatcaaca ccattcatca
    32641 tcaataagtg gttcgtagag gcatattgtt ctgcatccgc taatttttgc ttccaaaagg
    32701 caagagctgc ttctttttct actggaatct cattcccgtt gctataccaa ttggcaaata
    32761 gcagaccaaa gattgctgtt tgatcaggtc ctttccacca catctctgag tattgggaag
    32821 aatagttttc tgcttccaaa acttgattat caaagccaat tggttttaca ccacggccaa
    32881 acgctgctgc tgaaatacca gcttgtttca ttaattgagg cgtctgtccc atatttccaa
    32941 atgtatcggg aaaatagcct aacatgacag gggttcccca tttacggctt tcttccatgc
    33001 caatcagcat atttctcacg ttcgattccg agctgataag aaaatcatct tgcaagatat
    33061 aaaatggtcc aattctcaat tttccttcat tgattgcttg ttggattgcc tgtctttttt
    33121 ctggccttac ttgaagatag tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt
    33181 tgaaggacgg atcaacttca aatagctcta gtagatcgtc tatcaattca atcagtcgca
    33241 tgtgatgctg ttcgtaagcc atgtaccatt cacgatccca gtggctatga gaaatgatat
    33301 atactttctt tttcatttac gatacctcct acttttctac tctaatatca aagtaatcca
    33361 tgactaattc gcaaaacatc atatttgccc aagaaaacca ttctctcgta taattttcag
    33421 gattgttcac atcgaatcct tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta
    33481 aattcaatat ccgctctttt tcagatttgt ctgacgttgt cattccttcc atagctaatg
    33541 caatcggcca aacgtatttt tctggcgtat gggaggatcc aattcctttt gcgtactttc
    33601 cttcatagaa atatggattt tctttactca acaatgttct tcttgtttgc agataatctt
    33661 cctcttgttc agaacaatat cccaagtaag gtgcggcgat cagattgggc acattactgt
    33721 catccatgac tgtggcgttt cccattccat ctacttcata tgcataaatc gtttgccctt
    33781 tttgattaac cgttcgtccg tgattctgaa tcccctcttg gatctctttc ttcaatttct
    33841 gtgtttgctg tacaatttct ttatcatcga gtatcgtttg aaaaatttct tccacataac
    33901 ctaacacgac caccgcaaac atattggatg gtaccagata tccataacgg caagtatcat
    33961 cgctaggacg gaaaccagac catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt
    34021 cattgatcag cgtatcttct tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt
    34081 catgatcttg ctctgttttg aacacgtcca agattttttt gataccttca ataaaagatt
    34141 gattgaattg atcagttttt cctgtatttt tgtaaagaag ataggccaac tgtattgggt
    34201 agcataaaga atcaatttca tattttcttt cccagatcca atcgttcatt tttgtatgat
    34261 ctgtttggtg gccagcaccg ttagcttctt cgttgaaggc attcgcatat ggatcgatat
    34321 tgatataaaa aaattgcttt ttgactaacc cgctgatcat gtcagctaat tcctcatctt
    34381 cttttgcaat cactaaataa ggacgaactt gtgcagtaga gtctcttaac cacatcgctg
    34441 gaatgtctcc cgtcaataaa aaggtggttc cgtcattttg ctttttgact gttgtcaaca
    34501 gagtattggc aaatgctgct gtaaaattct ttgcccagtc cgcatggata tccccacatt
    34561 tttgtgtgat atcttccata aatttctgca ccgattttgg tatatcttta taagccacta
    34621 ataaaacctt ctttctattt gatatatctt taaagattat acttcataca gccaattatg
    34681 tctacgcttt caaaaaatat ctttttattt actttttcat ccattatggt attatttgaa
    34741 tatgatatat cattaagagg tgtttatatg aatcaaccat tgtaccagac aatatttaat
    34801 gatttgcaaa cggctatttt aactggtgaa ttacctgtag acagccaact accaactgaa
    34861 aaagaacttt cacgtaccta taaagtaagc agaatcactt ccaaacgtgc attgactgaa
    34921 ttagagcaat taggacttat ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct
    34981 tctccggaac agattcttgc ttctcctaca aaaacacgac ggattctgtt tcttcttcca
    35041 tatgtcgctg atttgtctgt tggtgatttt agcaagggat tgaaccctgt gatgcaagat
    35101 aaacagattg atgtcatgat gacgacgctt gactttctac aaaataaaac agcaaaagag
    35161 ctaatcaatg aatttgacgg attgatttat tatgctcttg atacagaaca acatcttgac
    35221 ttactttttg agctttcatt gaaagaattc cctgtcgtca tattagataa aaaaatctat
    35281 gagttacctt ttcctaccgt tctttccgat aatttccaag gcggtgcgct tgctgcctct
    35341 tatttgatcg aacagggaca ttcaaaaatc gcttatttgt ttggtacgca gactcatcca
    35401 caatccgttc gacagcgtta ccttggttac cttgaagcat tggacaaagc tggtttgact
    35461 ttccacactt cactggatga caaaagcgcg acattagatg aattgctttc ttatatcact
    35521 aagtatgaag taactgcttt tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat
    35581 ctcttgagac agcatggtta tcatatccct gaagattttt caattatcgg atttgatgat
    35641 atccaagcag cagctcttgt tgacccgcca ctgacaacca tcgcacaaga ttttgaacag
    35701 ttaggtaagc ttgcgggaga tgctttggtc acttggttag agacaaaaga tcgaccatcc
    35761 gatagtaaat ttccagtttc attcatcaaa agacaatcaa caaaggagaa ataaaatgaa
    35821 tatcggtcaa atagatacta gacatgcaac agcgaatcag ccaaatttct ctaatgggaa
    35881 ctgccagcct tatactggcg ttccttttgg gatgaattat tttgcaccgc aaactacgga
    35941 tcaaaacggc agctggtggt ttcatcctga tgaccgcgtt tttcaaggct accgtcttac
    36001 ccatcaacca agtccttgga tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg
    36061 aaaactacag gagaacaccc ttttccatgc tcaaagttct taccgcccag aagaaagtat
    36121 attttgcccg actcatctgt cgatctgtca attacgttat ggcatctgtt ctacattgat
    36181 acctagcatg tatggcggaa tcttatctat tgattacagc aaaaatgaca gcggtttact
    36241 tttatccttt ccgggacgcc atcagctctt tgttatagat ccttacacag tggaaggaaa
    36301 aatcacacat tttgctggtt cagaagatcc tgatttttct ttttatttta tattgagatt
    36361 tgaatatcct ttaacaattg atgagttagc tgtttccaac aaaaatagcg actcgatccc
    36421 gttgtatttt gaacagacaa aaaaacaaac tatccgattt gggacatctt ttatcagctc
    36481 ggaacaagca gcttataacc tggaactaga acaaaaccaa agcaaagaga cttaccttgc
    36541 tcacagtcaa gaacaatggc agtcctattt tgatcgtata cagctcacac accatgatca
    36601 acaacaattg cggacgtttt atcataattt atatcgggtg tttttatttc cacaaacatt
    36661 ttatgaaatt gatcctagtg ggcaaaaaat ccactatgat acgactagtc gatctataaa
    36721 acctggtgtt ctctatacaa ataatgggtt ttgggatacg tataaaacgg tttatcctct
    36781 ttattctttg attgctgtag aaaaatacga agaaatgctg gaaggttttt tgaacagcta
    36841 tagagaaagt ggctatttgc caaaatggct gtctcctgat gaacgtggac ttatgcctgg
    36901 gacattgatc gatgcggtca ttgcagatgc tgctgtaaaa gatatccgac gagacttgat
    36961 gcctgaattt ctaacggcaa tggaaaaagg tgctacggtt caaagcaaac atccaaatta
    37021 tggacgccaa ggaacagaag attacttgac atatggctat gttcctaatc actaccacga
    37081 atcagtcaat cacacattag actatgctta tagcgatttt tgtattagcc aagttgccaa
    37141 ggtactagaa gaagaggaaa tgacgaagaa atatcagcag caatcattga actatcaaaa
    37201 tatctttgac tatgagtcag gatttatgcg ttcaaaagat aaagatggca agttcagaga
    37261 accattttca gctaatcgtt ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag
    37321 ctttgctgtt tatcacgatt tttcaggact gatcggaaaa tatggtggca gtgagagata
    37381 taaagaaaaa ctcattgagc tgtgcaatca gcccccaacg tttgacccag gaggatatgg
    37441 ttttgagatc cacgaaatga gtgagatggc cgcgatagaa tttggacaat tagctatttc
    37501 aaaccagcca agttttcact atccttactt gttcagttat ataggacaac ctgaaatggc
    37561 acagccactg cttaaacagt tgatgactca gaccttcgac gattctccaa tgggttaccc
    37621 tggagatgag gacaatggaa gtatggccgc ttggtatatc ttcaatagtc tgggatttta
    37681 tcctgtcacg cctggaaccg gtgaatatgt tatcggtatg ccattaattg acaaggctgt
    37741 tttgcttttg tcaaatggaa aggaattgac gattgaagca acgcctaatc ggccgcagca
    37801 acaatttatc cataaaatca aacggatgga caaagaatat acgaaattat attttaccca
    37861 tcaagatttg ctgaaaggcg gcacgatctc ttaccaatta gggatcatcc ctcaccctaa
    37921 gaaatatcaa gcaaaagatt tgcctttttc cttatcaaac tataattaac aagaggttgt
    37981 gaaaaagctg tcctgcatca agtaataaga acagccaagc aaaaatagat cctcatattt
    38041 ttcgtttggt tggtcttatt accgcagatg caagcttttg aacaccgttt attgagaggt
    38101 tgtgaaatca gcgttctgac ctgagtatta gagagacaaa tggcaaaata gttcctcata
    38161 ttttgccatt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tagtggagag
    38221 gtcgtgaaaa agctgtttag tttcgagtat taagaaaaat tttgtgaaaa tggctttcca
    38281 catttttgca taaatttggc ttaataccga gaaactggct tttgaactcc gtttatcgag
    38341 aggtcatgaa aaagctgcct tgcctcaagt aataagaaca gcccaaaatt agatttctag
    38401 acaatttttt ataagttatt tcattacatt ggctttttct tttatgattc tccactgttt
    38461 tctcggtact ctttagggga tctgccgacg attttcttga ataatttaga gaaatacgtg
    38521 gtgttgttgt agccaatctc acttgctatc tcattgatgt tttgattact gtgcaaaagc
    38581 agttgtttcg ctttttccat acgtaaatgg ttcaaataag ctgaaaaact ttttttcgtt
    38641 tcttttttga acagctgtcc tagatacatg acatttaaat gcaggttttc tgacacttct
    38701 ttgagtgtaa gtggttcttg ataatgctgg tgcaggatta cgattacttt ttccacattg
    38761 tcactgtact ttttctgtct ctgatgcttt ttgatgaatg acagaagtag ctgctgaagt
    38821 tcttgcaccg tttttgcctg atggattttt tcgattcctt gtaaatattc ctcatcttcc
    38881 agatcaatca attcccgttg aatctccata aacagtaaaa aactggaata tcgtatatct
    38941 tccggcatca tggctgctaa ctgaaactgt tcaaaaaaat cagtgatcat tttttgagcg
    39001 acttccaatt gtttattttg cagcacgcgt ctgaaacttg aaaaatcgat ggtttgatct
    39061 tttgtttctg cttgtactgc gtaaaaaaca tgctcgtctt tatctccata aaattgatgc
    39121 aactgtagat tgtctttcgc ttgttggaat gtttcaggga ttttgtccaa agaatctgtc
    39181 tcttccccga tactgatcag ccattcctga tcaggaaagt tttcttgaac aaaacaacag
    39241 aagccatcaa cttcatctgc ctccaacaac aacgtgatca atacacggtc tccgtaattt
    39301 cgttgatagt aaaagatttg ttgccgttct tgcaaccatc tattgataag agtatcatga
    39361 gttcttgcca gttgaatcaa tactatccta tgctgcttct cacttatttg tcctattatt
    39421 tcttcttcac ttatttcatc caattcttca ttcaaccatt gactgaataa cagttcttga
    39481 tagatttctt gttgattttt ttgttcattt tgctgatcta accgtgtttt gattttcttg
    39541 aggctttcaa ccaattcgaa cttattcacg ggtttcatca aataattcac ggcgcctaat
    39601 tgcatacctc ctttcaaata atcgaattct tggtatcctg ataaaatcat aaattcaaaa
    39661 ttatgacgtt ctttttgagc cgcttcaata aattccagtc catttatttc tggcattgtc
    39721 acatccgtta ctactaaatc aatctgtcgc tgttcaagta cagctagtcc ttctttggca
    39781 ttctctgctg ttgctactat ttgaaagcct aaactttgcc aatcaattat cttttttaac
    39841 ccattcaaaa tcatgtattc atcatctacc aataacactt gatacacgct catccctcct
    39901 tgaatgtaat agaaacggtc agtccacctg tctcattatt ctcaagcgac atataataac
    39961 tgctgccgaa gctggcacgt aaacgttcat tcacgttttg cagaccgata gaaccgtgaa
    40021 gttccacttt aggatgagac agtttttctt caatctgttt tagccgtttt tcagtcatcc
    40081 cttttccatt atctttgatg atgatcctta cccgtttgcc ttcctgcaag actttgacac
    40141 ttagcgcatt atcgaatcga gtaaaatcaa taccgtgttt aaaataattt tcaactaatg
    40201 gctggatcac aaatttaggt acttcgatct tttccaaatc aggatcgatc ataaaatgat
    40261 aggctactcg attaggataa cgcatttgat acaaatagac atatttttcg caaaagtcca
    40321 gttcttcttt taaagtgatc gttttctctt gattcgtatt gtttcgaagc aaagcggaaa
    40381 acgcatacac aacatctgct aattcttcac ttccttcgct gattgcatac attcggatat
    40441 attctaatgt attatataaa aagtgtggat tgatctgtgc ttgaagcgcc cgcatatgtg
    40501 cgtcttgctg tttgatttct aatttgtaga tgtcttctac aaactgattg atgctatcca
    40561 acatttcgtt gataccgatt gaaagttcct tcagttcata ttccgtgttt gttatgtcga
    40621 tccgcgtctc taaattccct gctgctgttt gatccatggc ctgcatgacg atagatactt
    40681 gctgtgaata tcgtttgaat gttttataaa gaaagataag cagtatattg tctaaaataa
    40741 tgataccaat gatcagcggg gcaagatttt ttcccgtttc gatttttaca ctttttttat
    40801 ctagtacagc taatatacga taatcatcag acagttcttg gtgttgaaca aaattgtttt
    40861 tttccagatc gtcaatcggc aattgtgaat ttttcttcag actctctgtg atgatcattt
    40921 cttgtctttc aaacgtttct ttagctattt tattatcatg gaatgtgtat agtcgatttg
    40981 tcgttccaga aatcatgtat aaagagagtc cattgaatga cgttagattt tctaaaatca
    41041 tatctagttc tttcttttga aaaccaataa aaatacttcc tgactcagct cctccttgtg
    41101 tgataggcaa cttgatataa aaggcgtcta gtaattttgg tgtccccttt tttatttccc
    41161 cacctttgtt ttctctagaa gattcataat actccggtag ttgattcaac acgatatata
    41221 tcgcacttag ttcttcataa tttgcgtaga gatctttgat ttgatttggg aaagaaacta
    41281 attcatcggt actgttttgc tgttcgtata catacatcag atactgatct atgggttttg
    41341 ttaagtaggt cgtcacattt tcgatctttt cggaattccc tacaagctgg tttttgatga
    41401 tcctactcaa ggttgtttta tcattcagca ttctgcttgt gctttgaacg gcatcacttg
    41461 tcatgttttc tgcctgtttt tgattttgtt cccaagtatg gatactaaaa ataacggtag
    41521 cagtcatcgt tacaccgatc atgataaccg cgtatttccg caacagacga tttagtaagg
    41581 ttccttttga aaatagaatc cgaaacttat tcataacttt ccaactgttt atctaattct
    41641 tcacgccaat gtgttgtcat cgtaccattc caaaccgtca aaagaccaat gacaccaaac
    41701 aggatcaatc ctttgaattg ccaagtcacg cctaaaatta taccgctacc aataattatt
    41761 tttagaaaag acgaaaagct ggaaaaaacc gaaataaaac ttagctttag cagattagga
    41821 acagagatcg tatattcact atctaaaatc atactgtatt gataagtagc aaataggcag
    41881 agcaaggcaa aaagtaaaat aaaatcaata gctaaaaaaa tgagcccttt gatttgaaca
    41941 gataaatata aattgtaact caataaagca gtaccagata aaaataaact aaatagcaga
    42001 ttgcctctta aaaaatttct tttatatata ttccagcctc tttttattgt gatttcttta
    42061 tactcgaatc cgtacaaata aaatagttca ttgactgttt tccatgccgc acctaatcca
    42121 aagatgatcc cgccggcaaa tgtatatatc cagaaataca aggttagttt tataatgacc
    42181 cagatccgga taaataactc ttccaaaacc ttccctaaca tatcttctcc tccaaacatt
    42241 aagtttatac tttctcatta tacaaaaaaa ggttgggcaa tcagcacctt gacctgatta
    42301 ttagagaaac aaacggaaaa atagttcctc atattttgtc gttttgtgag cgaataccga
    42361 gggttagctg attgaacacc gtttaccaag agtttgtgaa atagccgctt agtttcgagt
    42421 agtaagaaaa attttgtgaa tatggctttc cacattttta catatattta gcttaatacc
    42481 gagaaactgg cttttgaaca ccgtttattc ggaacaagag gttgtgacac gtcttttgtc
    42541 acaacccctt tgtctttatt cattattatt cttgtcggaa ctcatcatat tgtttttgca
    42601 tttctttcaa taccttatca tagccagcct tgtccaaagc gtctttcaat ttcggtaatg
    42661 tttcatctgg atcaacagtc cctgtattca atccatctaa atattgattc atgacattag
    42721 aaatattact taattctgtt ttgacactgt cagtgttgaa gctgaatcct aaaatcggtg
    42781 aagtttctgc atcagcaatc gattgatcac gtttggcaat catgtcatcc gtaatagact
    42841 cttgtgtata tagaatcttg ttgtttcctg tattccaagc agacatatgt gtatttggct
    42901 gataaccatc tagcaatttg attttatcat ctgaaccatc gactttttcc catgcttctc
    42961 cctcgatccc ccaaacaagc ccattcaata attctggatc actgttaagc agccctagaa
    43021 gttctactga tttttcttta ttttttgaag tatttgatac gacaaaattt gccatttgtg
    43081 cttgagctgt agattttagt ggaacagtaa ttgcttttga gacaagttct tgttttgctg
    43141 catttgtcaa aattgtatcg ccataatcat acggtccttg tgtttctcca cgcattagcc
    43201 aagtatttcc ttctagtgga taatccttat tgctcgttgc agcgtcactt ggaatgagtc
    43261 cttgtttgta ccagctatgc attgtacgta acagtccgat atacgtttca ttatcgtatt
    43321 ggttgatgat tttcgaatca tctccatcca gatcaatagc aaatggtaac gtattcccta
    43381 aaggataatc aaagttccct tggactttga agccttgtcc aatggcaaaa gctgccgtgt
    43441 ttggttcttt ttcatggaat tccttcaaga ctttttctgc atcagcgtaa gattggattc
    43501 catcaattga aagatcgtat ttatctagta aagctttgtt aaaagtaagt acttgttgag
    43561 caaagacatt tccattgact gggaaagcat ataattttcc attgaccaga tttcctttaa
    43621 tatatgcttc atctaaatca ttataagctt tttcagcgta cttaggcgct aattcagtca
    43681 aatcagcaaa ggctcctttt tgggcattgg aaacatagtt gttcgcaaag gcgatatcat
    43741 aattttcccc ggaagaaatg atgacgttca ttttcttttc gtaatcgccc cagccaatgt
    43801 aattgatatt gacttttaca ccgatctctt tttcgattcg tttgtttgct acttccatca
    43861 attgatcgaa attatctggt ttatctccga tttgatacat ctgcaaagtg tccgaattgc
    43921 tagcttctgc ctggtcttta gaaccagaac ctccgcatgc tgctaatgtt cccaacaaca
    43981 acgctgaaac acctgtaaag gctagtttct tccataactt catttttctt cctcctaatt
    44041 ttatgattta tatttttgtt tctttattct tttactccgc cgattgttag acctttgaca
    44101 aaatgcttct ggaaaaatgg atagctgatc gcaataggca acgtcgagat gactacgatc
    44161 gccattctgg cagattctcc tggaactgag gctaatccgc ctgtcaattg gcttcctgct
    44221 cctgaatttt gtgtcagata ttgaatatta ctttggattt tcatcaaaag atactgtagt
    44281 ggaactagct tatcatcttg gatgtatagt aaagcattga accagtcatt ccagtaaccc
    44341 aatgcggcaa ataaactgat tgtcgcaata cctggaacag ctaacggtaa aacgatctga
    44401 acaaatattc gcagttcgct agctccatcg atccgtgccg attcaataat cgaatcagag
    44461 accgaacgtt taaagaatgt gcgcatcaca ataatattga agggactgac agccattggc
    44521 aagatcaatg cccaaatcgt atcttttaac tgaagaagat ttgtcataac aagataattg
    44581 gccaccatcc ctggtgagaa caacatcgta atcaaacaaa aaacggtaaa aaaacgacgg
    44641 aatggaaagt ttgaccgtga aatcgcataa gcatataaag aagtagccgt gctattgatc
    44701 agtgtaccta aaacagtaac aagtacagtg acgaaaagcg cttggaaaat tttatcctgc
    44761 atctggtcga acaagtacgt ataaccaaat atactgaact tttctggcca aaaactgtac
    44821 ccgtgagtcg acaatatgga ctcatcggta aaagagatgg cgataacaaa tataaatggc
    44881 aaaatacagg aaatagcaaa gagtgcaatc attatattag agaaaaaatt agccgtctta
    44941 ttgaaagagc ggacttggac tctctccact ttcttttttt tcattttttt cctccttaga
    45001 ataatgcaga atcgctatca aaacgtcggg cgatcgtatt tgtgatcatc aacaagacaa
    45061 agccgacaac tgattggtaa agtcctgcag cagccgtcat accaaaatct cctgtggatg
    45121 tcaggccatt gtagatatac gtatccaaaa cttgagtcac atcatataat gcaccggaat
    45181 tcctaggaat gttataaaac aggccaaaat cagctcggaa aatgtttcct acggctaaaa
    45241 tcgttaaaat cgtcattagt ggaataagtt gtggtatcgt cacatgtttg atctgctgcc
    45301 atttgctagc tccatcaacc atagcagctt cataataagt cggatcgatt cccataacag
    45361 atgcaaagta aataatgctg ttataaccaa tccctttcca tgtaccaatg aacaccaaga
    45421 taaacggcca atatttcggt tcattatacc aattgacagc ttctcctcca tgtccagtga
    45481 tccactgatt gaatattcct ttgtcaggac ttaaaaaagc gtaaacaaag taactgataa
    45541 tgacccaaga tagaaaataa ggtagtaagg acatcgtttg ataaacttta acgagtcgtt
    45601 tatttctcaa ctcgctcata atgattgcaa atacgactga gatcagcaga ttcaaaagga
    45661 taaatgctac attatataaa attgtatttt tagtgatgag aaatgcttga tccgaagaaa
    45721 ataaaaattt gaaattttcc caacccaccc aaggactttc cctcaagcta gccaagaacc
    45781 catctgctga aatatgaaaa tttttgaatg ctacgacatt cgccattaca ggtatgtaaa
    45841 agaaaaaaat catccaaagc atacccggta cagccatcag gacaagtgct ttataacgcc
    45901 atagattcat aaaaaaatga ttgaacccct ttcgtttttt catctaaaaa cccctttcga
    45961 ttcattcgca acgtaattat agcgctttca ataatgagaa taaataaaca aagtataggg
    46021 aaaatgaaac aaagtatagg agaggttgtg aaatcagcgt tttggcctga gtattagaga
    46081 aaaaaacggg aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag
    46141 ctgattgaac accgtttatc agaggttgtg aattcagcgt tttggcctga gtattaggga
    46201 gatcgtgtcc ttactatcta actctgagtc acaaggaaca atggggactg taacaaaagt
    46261 cttgtcacag tcccttattc cgaacaaacg gtgttccaga agtagcttct acggaaataa
    46321 gccgaaattt cacaaaaaag ccatccgaaa gtcagacgga caagtctgct tttcagatgg
    46381 cgctacaact actctgctta ttcttttagc tatccattca attatttttc tctcacttgt
    46441 tctagctctt ctttggtcat gggtcgatat tgccctttat tcaaatcttt aggcagccaa
    46501 aattttccca tacggattct ttttaaatat aagacttctt ttcctactgc tttgaccatt
    46561 cgcttaactt ggtgaaactt tccttcatgc aaaatcagac ggataattga agtttctgtc
    46621 tcttgatcta cttgatcaat ttggagtttt gccggcaatg tctgctcatc gccatcaata
    46681 atcagtcctt ctgcaaataa acggatatct tcttctgtca ttataccgct aacctcagct
    46741 agatattctt tctccacatg ttttttaggt gatagtaact gatgggcaaa caccccgtca
    46801 ttcgttaaaa tcaagaggcc ctctgtgtct ttatctaatc ggccgactgg aaaaagatct
    46861 tcccgataat cctggtcttc caataaatcg attacggttt gatcatgatt atcctgagta
    46921 gcagaaatga ctcctgcagg tttatgcaaa atgtagtaaa aatctttttg atacgtaatg
    46981 atttctccta aataagtaac cgcatcttcg aattcattta cttgcgcttt atcgcttttc
    47041 accacttgtc cattgacttc gatcgttcct tttttgatta atcctttgac ttctttgcgg
    47101 ctgccaagtc ccatatctgc taaaaactta tctaaacgca tgcgtttccc ccatttcaaa
    47161 agagcatcaa tctagctctt ctgattcact tattttattt ttaatttatg tcgtaaacgc
    47221 gctattcctg gtcctagtag tctttctgcc aagcggattt tcaaagctaa ataaccatac
    47281 actaaagccc ctatacctgc tactacaaga atcaacaata gagactggaa tttgctatct
    47341 tcgtttaaaa aagcaccaaa aatcatcttt gtgatccacg aagccaaaag cataatcaag
    47401 gtaagaataa agatcagcaa tgtcctgcgc cagacaaaac ttggtttaaa gcgggctact
    47461 tcatggattc tctttaagat caaggtacaa gacacagcaa atccgatcat tgtcgccaat
    47521 aaagggccgt atacttcaaa taaacgaatg gctgggtatt gaagaaggag tttcactaga
    47581 aacccaataa ccaaataatt aattgccgcc ttgttttcaa acattccttg cagcatgttg
    47641 gaaactagca tatatagtcc taggaagagt cctacaaaac ttgcttggat cagtacatta
    47701 cttcctagag aatccggtgt ataaaataat gtatttagcg gataggcaag taacataaca
    47761 ccaaatgtcg ccggaaacat aaaaaatgaa aacaactgta aatttccgct tgtcaatttt
    47821 gccagaccta ctcgatcttt caacgtaaca gcttctgtaa tcaacggtaa cccagtagac
    47881 gcgatcgatg ttgccaatgc aataactacc atcgtcaact tatctggatt ggcactaaaa
    47941 atcgaaaaaa gatccagtaa ctgtgcattg gaatactcgg tggatgcaga catgatcttc
    48001 ataaaagtga actgatcgac taatttgaag atcgttaccc ctgaacccac aataataaac
    48061 ggaatcgctt ctttaacggt atctaacaac aattccttcg ttgcaatatt tattttgttt
    48121 tcgctgtagt gaataaacgc cgatgtatac gcttgatgtt tcttcaaaaa atacaaaagg
    48181 acaagaatac ttgctagcat accgataaat gccgcgaaag ttgattgcgt gaccgccgtc
    48241 acatagtcac catccatgac tttcatgatg atgaatgtag ccaatagcat ataaaaaata
    48301 cgtgccacct gttctacaat ctgcgacagc gcataaggca tcatttcttg attgccttgc
    48361 aaataccctc gtatcacact catacaagga aaaaccaata cagcgatact caaagaacgc
    48421 atcgttggaa tcagttcttc tccgccacca gaagcatgag ctagccaagg agaggcgatg
    48481 tacatgaaca aagcaaacaa caggcctaac ccgcccatca tctgtaatgc acggataaat
    48541 aatcgtcgtg aagtgccata ttcattcaat gaattatagc gtgcagtttg ttttgcaatc
    48601 gcagcaggta ttcctgctgt agagatcagt agaaacaatg catatatatt gtatcccata
    48661 ttgaataatc cattggcagc cttagcattt tcgcccatcc aagcatacca gggaatgata
    48721 tagacagcgc ctagcaatct tgaaatgata ttactggcgg tcatccaagc agagcctcgt
    48781 gccatttttt cttgcggtgt taattgatct aatgctggtt ttctttgatt atccatcggt
    48841 cagattccaa ctttctacaa aacattacta tcattttaat tggtaaaaca gaaactggca
    48901 atcgatttta tataactttt ataagaacaa ttgttttttt gaattcggtt tcgttttttt
    48961 ggtaaagtaa agaaatatac agccttttat ggctaacatt tataggagat atcaatgaca
    49021 caagaaataa aaaaactacc cgttatattt ttaggtcttt tctttatcac agttggattg
    49081 aattggttct tgctccctca cgatatagct tccgccggag tgggcgccat cggacattta
    49141 gttgaaacgg ccttttctat ccaacgctca gttactgttt ggagcatcaa tcttatgatg
    49201 ctgcttctag ccgctgtcct attaggaaaa tcagtatttt ttaagactgt agcgggaagt
    49261 ctgctttttc ctgttgtttt agagattgtt ccaaaagtgg aaatgatctc ctcacatttc
    49321 ttttcactga tttcaggaag tcttttgttc agtcttgggg tttacacatt gtacacagtc
    49381 ggggcctcaa atggcggtat aacgataccg ccgattattt tccacaaatt ttttcggctt
    49441 ccaatgccgc aaggactttt acttaccaat agtcttatcg tctttttgaa ttattatgtt
    49501 tttggattgt tggagacact attcgttctc ctatctatta cagtcagttc attgtttatg
    49561 aaaatactca tccgcttgtc ccctgtgtca agaaaatttg aataaagctg gattttgtca
    49621 tagcggaaac aaacgatata ttaaaaagag tgtgctataa taaaccaaga aattgaataa
    49681 agcgagggat caatcaatga gcttctcttt agaagacatc cgtcatttac tattaaaaga
    49741 aaatctatta aaagaatttg tttcaacaag aggctggcat cttgatgtac cagaaaaagc
    49801 tgaatttctt cagttaagtt atgattcaag aaaagctgat gcttctactt tgtttttctg
    49861 taaaggaatg aatttcaaag aagaatattt ggattcagca atcgaacagg gtattcaata
    49921 ttatgtatca gaacaaccat acgaaaattc agcagtagga atcatcgtaa cggatatccg
    49981 taaagcaatg gcgcttcttg ctatggcttt ttacgactat ccgcaaaata aattgaaagt
    50041 gatcggattc actggaacaa aaggtaaaac aactgctgcg tactttacaa aagcaatctt
    50101 ggatcacaca acaaataaaa aaacagcatt gctttctact atgaatacaa cgttagatgg
    50161 caaaacatat ttcaaatccc atttgacaac gcctgagtca ttagatttat atcgtatgat
    50221 gtcagaagca gttgaaaatg gtatgactca tctagtgatg gaagtttctt cacaagctta
    50281 caaaacacaa cgcgtatacg gcttgacttt agatgtcgga atcttcttga atatctcgcc
    50341 tgaccatatc agtccgatcg aacatccgac atttgatgac tatttctatt gcaagcgaca
    50401 attgatcttg aattcaaaaa cagttgtttt aaatcatgaa agtgactatt ttgatttact
    50461 tagggaaaca gccgatctat tccagattcc aacgatcact tatggtcgtt cggaagaaag
    50521 caattatcaa gtgatccgtt cagataaagg tactcacggt tttactttat cttctcatga
    50581 agatcgctta gctatctgcg atactgctta tgatattttg ctggctggag gattcaacca
    50641 agaaaatgca gccagtgcga tcattgccgc agcactaaca ggtgcctcca gtcaagatgc
    50701 acaagacgga ttaaaagaag ctcgtgtacc aggccgaatg gatcaattga tccaatccaa
    50761 tggagcgcat gtctatgtgg actatgcaca taattatcta agcttgaaaa cattgctgaa
    50821 atttgccaaa aatgaacatc cagatggccg tgtaattgtt gtattaggca gcccaggaaa
    50881 caaagcaatc tcccgtcgac atgattttgg aaaagtgcta tcagaaacag ctgacatcgc
    50941 ctttttgacg gcagatgacc cagcatttga agatccgaaa aaaatcgcgg aagaaatcaa
    51001 tgaagcaatt actaatccag atttgatcgt tcattatgaa atggatcgtc ctgaagcgat
    51061 tcgacgcgct ttagcagaaa gcgctccgca agattcagtc gttattgcgg gaaaaggcgt
    51121 ggatccctat caaaaaatca atggtgtaga tgaaccttat gaaggggatt atgcaatcgt
    51181 gaagcggttg attgaagaat aatggtattt atatactagt ggtttcccat ttctaagaaa
    51241 aaaagggact gtgatttttt gtcacagttc cttttttctt agaaatcatt ttaacttttc
    51301 actagctaat tctgctgcct tttctctttc ttcatttaat aatttattat catagaattt
    51361 gataaatggg aaccagataa tgaatgcgat gattgcacaa atgactgcta acacagcagc
    51421 tttccaatca ccaccactac caacaaatgc tccgatcccg actggtgaag gccacggcat
    51481 ctgtgcaagc atcggtttga cgatttccag ccgaatggca aaataagcga gtgtagcaga
    51541 agccatcgga gctaagaaaa acggaaccgc taaataggga ttgtagacga ttggcatccc
    51601 gaaaatgatt ggttcattga tattgaataa tcctggtaca agtgaggctt ttcctaacac
    51661 tttcagctga tcagatttcg cgaggaatgc aataaagatg atcaatccta aagtcgcacc
    51721 tgatccacca acggttacat aagaattgtt gaattctcct gccaacggat aatttgcgcc
    51781 ttgtgcgtta gctgccatat tagctaatac gatcggggtc aagaaagaag tgatgatatt
    51841 cgctccatgg atccctacga tccacaatgc atggatcaag aaataaatca ccatgacacc
    51901 aagccatgta ttcgtcaact gcgtaacaaa cccaaatggg atagcaatca ttttgaagat
    51961 atccgttcct agtgcaacta ataatccatt gatgatcatc accacaaacg caatgacaaa
    52021 tgttgggatc agagcagtga aagaacgaga aactcctggc ggaacaacat ctggcatttt
    52081 tatgacccaa tttctcttga cacacataca atacaattcg accgcaataa tcgacataat
    52141 aatagctgta aagattcctg aagttcctaa acgttcgaca aaagagccca tcctaatacc
    52201 atttactaca gtttctgtct cattgataga agcaatcaag ctgattttac catcagtcat
    52261 gatcaattca gggatacaca taaagaatgc aaaaacagat agtaatgccc ccgtgagcgg
    52321 attaacattt aatttttgtt catctcgttc aattgacgtt aattcataag caaacacaat
    52381 ggcaaagtaa atagataaaa tccccatcgt cattgtattg acgatcatgt acaagtcact
    52441 aactttgaaa aaagttgcat taaaaaatcc ttctaaaaat gggaacggta atggcaaaat
    52501 atttaaaacc aaaaacatcg agcctacaat cgtgaaagga atagaagcca tccccgctgc
    52561 catgacagcc cgaacgattt taagttgcgc aattttcccc ataggtccca tcaggtattt
    52621 ctgcaaaaaa tcaaacatta gttattctcc tttcctaaat ataacgcttt ttcatattgt
    52681 ttaatacgga tatactgttg gtcttggttg agtgaaattt tctttaaacg atactgtata
    52741 aatgcactgg caaaaatacc gcttacaaca agaatgaaaa caaataactg tcctccattg
    52801 ttatttgcca taaaaggata caagaatgta aatcctgagg taaacaacac tggaatcact
    52861 aataaattaa agacactctg gaacaaaaaa taccactttg tccatggaac gttcgtttgg
    52921 tgttttcgat aaagctttac ttgttctcct actgcaggca aaatagctaa aatcagaatc
    52981 atcggtaaac tacttcctaa acttttagct gatagaagca gaatcatcca ttgcatgttc
    53041 atgaaaaaga aaaaggcagt caggtaacgt acgattagat aacgattgaa atacatgttt
    53101 ttcaaactaa gttgcttttt ttctaattcg attttccttt ttactgtatc ctgcacaatg
    53161 atccttcctt tctatttatt tttttgatac aaataattca tttcctgtgc aacttccaat
    53221 aatgtcatcg tcgtcatcag atgatcttgt gcatggacca tgataatttc catttctatt
    53281 tttgtcccgc tggcatattc tttcagaagt tgagtttgtg actgatgcgc atgaagaatc
    53341 tcttcatttg cttgtcgcaa cttctcttct acttcttcaa acatctcttt acgcatcgag
    53401 cgaaatgctt catgtatcag tgtccgtgca tttccgctat ggaaaatgat atcaaaagaa
    53461 gtcacttgta gtgtttcact gtttattttt tcagccatct gttttcctcc ttattcgatc
    53521 agtttgataa atttactgct gaaattctca aaaataggtt cagctaagat ttcctgctgt
    53581 acttttagct ggtcaactaa cctaacgatt gctttagtca cgaccgtaat tccttcattt
    53641 tcgacataag aaggcgaaac taaaaaaaca aaatgaattt cttgatattg atcccactgc
    53701 atcccagaag gaatcaatgc aacggctacc tttgtttcga ttccaactgg aacagcggga
    53761 tgtgggactg ccactgtatc actgaatatg atttgtccca tattttcacg ttgctggatc
    53821 tgctttcgca tttctcttat atatccttct tcttcattta tgctcagctt ttgcagcaaa
    53881 tcatgaagaa tttcttcctt tttcggttcg tacgtataag tcttgaaaaa tttttcagac
    53941 aactgttcgg tgtaaatctg ctttttttgc tgtagagaaa atgctggtat cggtctatct
    54001 gaggaatgat agggtataga ttctccaact acctttcgta ttttacgaac atcatcttca
    54061 ttcaaaaaaa tactgacatg taaaactgga attttgaaaa acattgtcga aagatcaata
    54121 gaagaaataa tcaggtcgat tccttttaaa gttgtttcat tgatctcata atagcctttt
    54181 acacttacca ctgtaatatt tttaccgaat tcactgacta cgcgattctt taatagttgg
    54241 gcactgccgt aacccgttgc acagataatc aatgcatgta gtttatgcgc agctctttct
    54301 ttttccaatg ccgccatgag atgcaaagcc aaataggccc attcatcttc attgatggta
    54361 tagtttttca aagccggcat gttgctgaaa tgccttttta ctgcgtcaaa gactttggga
    54421 ttttctttct ttatctcctc agtcaatggg ttctcaatgc gaatacctcg gctcaatcga
    54481 atcatcatcg gtgacaaatg atcgagtagt ccattgaaaa gctggagatc ctctactaat
    54541 gaaaaatgaa attcctgaga caatcttttc agaactttcg ctaattcctt tgatagttcc
    54601 tgatcatttt gattttcttt gtgattcgac ttagccatga gatgtaatgt caaataagca
    54661 atttcttctt cgggaaacga tacacctgcg attacctcaa tacgttgaac gatccgctgc
    54721 gccacttgat actctactcg cttagaaact tcttgttcga tgcctaattc cttgatttca
    54781 aacccttctc ggagccgttt gatccccaaa gccaagtgta aagtcagatt ctgtatgata
    54841 aaatccgaaa cttttaactt agcttctctt gtttcatcga gaatgataat gactagctct
    54901 tcaaaactga tgtctgggaa aaaacggctt tgccctaaat attctttcaa tgaatttcca
    54961 taatggtttc caaaaaaagt atccatgata aaatgccgtc gatttctttc ttccccttcg
    55021 atccatgtcc cttgaccatg cttagaaacg atttttagtg aatatggaat caatcgctcc
    55081 ttgatttctt gcatgtcctt tgtcaaactg gaacgactga tataaagctc ttctgccaaa
    55141 tcatccataa aaagaatggc atcttccaaa agcaattggt tcaatatata cttttgtcga
    55201 tcttcggact cataaaatcc ttgcggttct ctagcatatc caggtactat ttcttgccga
    55261 ctcaaaaaca agtcaaatgt cagtctttgt aaaatatgca gttggtagcc ataacctggt
    55321 ttagcaatga tcttacctcc gtttttttct actaactctt tgatccgctg caagtaatta
    55381 cggattgtcc gatcagataa taacaattca gaagctaaac gttgactggt gacatactcc
    55441 ttcttgtgat caatcaaata tctcaacagc aatttttcct tctgtttcat tatcatccac
    55501 tcctattcca tcatatgttc tcttcgatca cctctgctaa cttctggatg cctgtaggga
    55561 ttgggacata cgcttggaaa gggatactaa caacaggctt tccaacagat tctcctaatt
    55621 ttttgaattt atccaaaaac atcgtcgttt gcgggctgat cagaaataaa tcaaaatcac
    55681 tattcttaat catattatcg ccttctgtag ctgaaactgc atctacgagt acctcttctc
    55741 ttttttgctt aaagaaatcc gttgtctttg aggccatcat cgaggatgac attcctgccg
    55801 cacaaataat caatgctttt ttcataaatc aatcactcct tttattttta taaaccaagc
    55861 atatcttgta agcgttttct attcaaaata tatatttccg tagcattgcg gaaaaaatgt
    55921 gtatcatatc ttgaataata atttctggca ccgtgatata ctagtgatag aattttttca
    55981 ggaggggatt tagtgagtat cacagtgaat attttgtata caagtacaaa tggacaagcc
    56041 agaaaatttg cagaagaaat ggtcgaaaga aacattgtca aagcgattcg tgcagaagaa
    56101 ggaaacgaga aatatgacta ttactttcca ctagaagatc cagaatctct tttactgatc
    56161 gaccgttgga aagatgaaga agcaatcgaa aagcatcaca aatccgaaat gatggctcaa
    56221 ataaacgaac tccgtaaaaa gtataaatta aaaatgaaag tcgaacgttt cactgattta
    56281 ccgtaaaaag atacacatgg aatgatagca atttatcatc catgtgtatc ttcacttttt
    56341 ccttattttt tattcgatct catctaatga ttcaaaccgt gtttttctct tatttttcgg
    56401 ataagtattt cagtcacccc aatatacatg attcctaaac ataccccaag gatattctca
    56461 aaaatgcgga tcgttactgc tcctggaacc ccgaataaac tggcagtgat tgccaaagca
    56521 ccaaaagaat taaaaactgt tttatatcta taggtagaac aaacacctaa agctagacca
    56581 cctaatatac cgacccagtt aaaaggaata aattggctga taccaataaa taaaacacat
    56641 ccaatcaatg agccaatgat tcggtctact gctcgttctt tgaaaccaat cgacatcaat
    56701 ccatagctag atacaataga agaaaatgca aaccctgccc acatcaatcg ctgaaaaggg
    56761 acatactctc ctgcaacgat caaaagacta atgccaaatg catagatgag catccaaatt
    56821 ttttcctgcg aatacatgcc tttcaaaaaa atctctttaa acaagctttt gtctttgttc
    56881 ttttctcgat gttttctgta aaggagaact gaaaaccaac aaaaaaacaa aaataataag
    56941 ctacttgttt gtgtaaagaa attcttgttc aacgaatctt taggtaacga ataaacaata
    57001 aagagatatg aaaagccgta caaagaagca ttccccattt tgggatcact agcggttgcc
    57061 attaatatgc tgcttagtaa aataaaatga atcaacatgc ccataactga ccatttgatc
    57121 agctggacat acggtgctac taatagtgag aacatgacga ttgctaaacc tatgatcgaa
    57181 tgggaaatct tataaccaaa gtcaacaaaa cgaatactca tcaacataca gaataaaacc
    57241 acagcgtatg gtttatgctt cgattcaaat aatgttgtaa ttataatgat aaaacaaatg
    57301 gcaaacaata cgatgaataa tgatcgtaaa aacaatgaac aaatatacat gcacttttct
    57361 tttttagaag ctgcttctcg tattttttgt tttaaaataa acggatctag ttgtagagac
    57421 tgataaaaat ccacctgttg tcgtcctttc tttttacttt tattttttac agtcatctat
    57481 gacaggttgt aaatactatg gaacatcata tggcgttgtc attttcttgt caactatttt
    57541 tcctattcat gtttttataa aaaaacatat cgttttcttg tatgattaaa tcaccacaaa
    57601 ataaacaact gagagaacga tatgctttat gaatggttct atcaaaaaaa ttctgagatc
    57661 aatagacaaa aatttgatga ttacagaggt ctcttacgaa accttacaga agaaaaaaac
    57721 attgatcgtt gctgcagttc tcttccctgc tccatgtgtc tgtagaaatt gtggttctgc
    57781 tgtggtagat ggaaatagga aattaatggt ggtgaaaaac aagaagaagg aaaccattgt
    57841 ccactttgaa taatataatc atatgccaat ggttatacgc ctaaaaaagc agcgctatac
    57901 ctgtaaaaac tgtcgaagcc attggaacgc tcaaagttat tttatccgtc caaggcactc
    57961 gatttcaaat catgtcagac ataaaatcac ttctttactg actgaaaaag tgtcgttatt
    58021 ttttatttcg aaaagttgtt aggtatcttt gacaaccgtt attcgcacat tgaaagagtt
    58081 gagaagctat ttaccaaagt cttccaaaag aattctgtct tgagtgttga gggttgtgaa
    58141 tttcgttctc acgtttccgt tgaggataaa ctgagtttta tttccgcgga tggcgagaca
    58201 gaaaaattaa tcgagaaaat ttacctacac aaaaaattcc tcgattaaca aactatttcc
    58261 ttagctgcac taatcctaaa gaaatagaat tcttagttac agacatgaac gccacctact
    58321 ttcagctcac caaacatgtt ctaccaaatg cgaaattggt gattgatcga ttacttggtt
    58381 tttccacgcc tttcaaggag gcgtaacctt tattcacgag ttggttgaag ctttccgaga
    58441 caaaagaccc gacttgtttt tctccttatt gacaaagagc cattaaaact agagaaaaaa
    58501 ttacaactga tcgctgatta tttgtagaaa gaaaatcaaa gtattaccca ataaacttga
    58561 gtcattaggc aaaaaacact gcgaaactca agaatgattt tcgcagtgtt tttgtatatt
    58621 ttttattaac tagtattact acaaaggttt atttttcact tctcaacgca gcacaaaatt
    58681 gtctttactt atttactggt tgggttcaac cttctctact ccgatactaa tatttgtttg
    58741 tatacccctt ctaaaatcgg tagtttccat tcaattgatg ggtactagtt gattttaaaa
    58801 actatgctta ttcaattttt tattttagct catatatttc gtttctttct aaattttata
    58861 ctgatgaaaa caagtagcac acccaatatt gacaatataa actggctaat gatggtttct
    58921 ccagtttttg gaagatgctt atttccactg cttgttggat tgtttcctct tatatctgag
    58981 ctatcagcaa cttttctatt ctcttttgtt aaaaagatcg ccacttcttg ttctttttcg
    59041 atcttgaaag ttactggtgt cgcatccaat tcataccccg ttggcgcttg agtttctacc
    59101 agctgatatg ctcctggtgc taagccatct atcgccagtt ttccatctgc tcctgtggtt
    59161 agacctgact gcagaacggt tccattggca tcttgtaact caaaaacagc tccttgcagg
    59221 acttctccgc tttggtcatc gatctttgtc aacacgacac cgcctggtgt aagtcgattt
    59281 tctttcgtca attctac
//
LOCUS       DACECY010000009        57159 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.009, whole genome shotgun sequence.
ACCESSION   DACECY010000009 DACECY010000000
VERSION     DACECY010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.009"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(59..187)
                     /locus_tag="IWO38_000591"
     CDS             complement(59..187)
                     /locus_tag="IWO38_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ0312707.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IWO38_000592"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IWO38_000592"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ0312708.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="IWO38_000593"
     CDS             complement(1674..2729)
                     /locus_tag="IWO38_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ0312709.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IWO38_000594"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IWO38_000594"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ0312710.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IWO38_000595"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IWO38_000595"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ0312711.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IWO38_000596"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IWO38_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ0312712.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IWO38_000597"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IWO38_000597"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ0312713.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IWO38_000598"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IWO38_000598"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ0312714.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="IWO38_000599"
     CDS             10491..12050
                     /locus_tag="IWO38_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ0312715.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="IWO38_000600"
     CDS             complement(12161..14092)
                     /locus_tag="IWO38_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0312716.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IWO38_000601"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IWO38_000601"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ0312717.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="IWO38_000602"
     CDS             complement(15021..15773)
                     /locus_tag="IWO38_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0312718.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="IWO38_000603"
     CDS             complement(15932..16141)
                     /locus_tag="IWO38_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312719.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="IWO38_000604"
     CDS             complement(16152..17480)
                     /locus_tag="IWO38_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312720.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(17845..18432)
                     /locus_tag="IWO38_000605"
     CDS             complement(17845..18432)
                     /locus_tag="IWO38_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ0312721.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="IWO38_000606"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="IWO38_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ0312722.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /locus_tag="IWO38_000607"
     CDS             complement(20012..21301)
                     /locus_tag="IWO38_000607"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ0312723.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="IWO38_000608"
     CDS             complement(21455..22387)
                     /locus_tag="IWO38_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312724.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="IWO38_000609"
     CDS             complement(22544..23203)
                     /locus_tag="IWO38_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ0312725.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="IWO38_000610"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="IWO38_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ0312726.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="IWO38_000611"
     CDS             complement(24244..24534)
                     /locus_tag="IWO38_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312727.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24944..26749
                     /gene="glmS"
                     /locus_tag="IWO38_000612"
     CDS             24944..26749
                     /gene="glmS"
                     /locus_tag="IWO38_000612"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ0312728.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26821..28176)
                     /locus_tag="IWO38_000613"
     CDS             complement(26821..28176)
                     /locus_tag="IWO38_000613"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ0312729.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28200..29369)
                     /locus_tag="IWO38_000614"
     CDS             complement(28200..29369)
                     /locus_tag="IWO38_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312730.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29366..30253)
                     /locus_tag="IWO38_000615"
     CDS             complement(29366..30253)
                     /locus_tag="IWO38_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ0312731.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="IWO38_000616"
     CDS             complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="IWO38_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ0312732.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            34320..34841
                     /locus_tag="IWO38_000617"
     CDS             34320..34841
                     /locus_tag="IWO38_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ0312733.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35540..36105)
                     /locus_tag="IWO38_000618"
                     /pseudo
     CDS             complement(35540..36105)
                     /locus_tag="IWO38_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36284..36940)
                     /locus_tag="IWO38_000619"
     CDS             complement(36284..36940)
                     /locus_tag="IWO38_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312734.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="IWO38_000620"
     CDS             complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="IWO38_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ0312735.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(38477..39475)
                     /locus_tag="IWO38_000621"
     CDS             complement(38477..39475)
                     /locus_tag="IWO38_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ0312736.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="IWO38_000622"
     CDS             complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="IWO38_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ0312737.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(40491..41222)
                     /locus_tag="IWO38_000623"
     CDS             complement(40491..41222)
                     /locus_tag="IWO38_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ0312738.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(41226..42491)
                     /locus_tag="IWO38_000624"
     CDS             complement(41226..42491)
                     /locus_tag="IWO38_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ0312739.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="IWO38_000625"
     CDS             complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="IWO38_000625"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ0312740.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="IWO38_000626"
     CDS             complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="IWO38_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ0312741.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            47833..49335
                     /locus_tag="IWO38_000627"
     CDS             47833..49335
                     /locus_tag="IWO38_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0312742.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            49337..50074
                     /locus_tag="IWO38_000628"
     CDS             49337..50074
                     /locus_tag="IWO38_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0312743.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="IWO38_000629"
     CDS             complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="IWO38_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ0312744.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="IWO38_000630"
     CDS             complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="IWO38_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ0312745.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="IWO38_000631"
     CDS             complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="IWO38_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ0312746.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="IWO38_000632"
     CDS             complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="IWO38_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ0312747.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(55574..56740)
                     /locus_tag="IWO38_000633"
     CDS             complement(55574..56740)
                     /locus_tag="IWO38_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ0312748.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(56756..56983)
                     /locus_tag="IWO38_000634"
     CDS             complement(56756..56983)
                     /locus_tag="IWO38_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ0312749.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
BASE COUNT        15681 a        12459 c        10029 g        18990 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat ttaattataa
    17521 ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc tccctgaggt ggcacgcttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcacgttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttcaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttcattt aacacggaac
    21661 taagcagcgc atcttcatta ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgct actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcatg ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaatgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccta
    22621 gatgttgctt tttctgatta aagattgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttga ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagatac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaagc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgcata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttgaagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt ctcttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgtcaatt catcttctac
    24361 cgtaaactca cgagcttcag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaagagcat ttgacaaagc atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaagagg aggatagtta taccttttag gatagcgcca ggtctgtgta
    24781 aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag ggactgcact
    24841 ctacagaaaa tccacaaaaa atatctgcaa agataaaaca aaaggatttt cctgcagctg
    24901 gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg gaattgttgg
    24961 aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa aattagaata
    25021 ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt atctggtcaa
    25081 agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta caggaaatat
    25141 aggtattggt catacacgct gggcaactca cgggcaacct tctgaagaaa acgcccatcc
    25201 acatacttcc caaagtggac gcttcgtgct cgttcacaat ggcgtaatcg aaaactttga
    25261 agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg atactgagat
    25321 tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg cgtttttaaa
    25381 agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga cagcacctga
    25441 tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg atggattcaa
    25501 tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg ttgagattga
    25561 agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt ttgcaggtga
    25621 catcgtagaa cgttcttcat tcgaagcaca agtagattct tcagatatcg agaaaggtac
    25681 ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc gtcgtatcgc
    25741 tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg tcgatacatt
    25801 gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg cggggttagc
    25861 aggtaaacag actctggaaa aattgacaca gatccctgtt gaggttcatt tagccagtga
    25921 atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct tgagtcaaag
    25981 tggcgaaaca gcagatagcc gccaagttct ggtaaaaatc aaccgtctcg gctacccttc
    26041 acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct ttacactgct
    26101 attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg cacaaatagc
    26161 tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa cttctcttgt
    26221 attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg tcatcgccca
    26281 aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa atgcctttta
    26341 cattggacgt ggcgctgact acaacgtttc tcttgaagct gctttgaaat tgaaagaaat
    26401 cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa cgattgcttt
    26461 gatcgaagaa ggaacaccag tgattggaat catcagtgaa gaagtgactg gtgcgcatac
    26521 tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta ttgtctcaga
    26581 gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt atctaactac
    26641 tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat tacggggatt
    26701 cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat aaaagaacaa
    26761 ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg aaaatatctc
    26821 ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat agtaattaac
    26881 tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac cggaaggacg
    26941 aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca cttttttgat
    27001 tgccggaaca tccatcgcac cgtttttatc acttactcgg atattcacta atttttgcgg
    27061 atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt tcatgatatt
    27121 cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta agaaaatcat
    27181 gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt ctacaacgta
    27241 acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg ctttgtggaa
    27301 tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat ttttttctgc
    27361 caagtattta gcacagataa acatgatttt atcaccgtcg acaatgtttc caagttcatc
    27421 caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg ctcctttttc
    27481 aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccatcat tgatatttaa
    27541 cccgtctgga tttgtgccca tggtataaaa atctgtttca agatcggcaa acaaacgatt
    27601 aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac cggaaaggtc
    27661 accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg gaaattcttc
    27721 tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag catctaataa
    27781 tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac cgaaaaattt
    27841 gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg cgcttgcttt
    27901 ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct ggaacacttc
    27961 gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac ctgagattct
    28021 tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct ggcttaggac
    28081 ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct tgttggcttc
    28141 accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac tcctttaact
    28201 taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc tgatgtttct
    28261 tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat ggttgttgtc
    28321 gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt attttcttgt
    28381 gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg tgtaatctcc
    28441 acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc aattttacga
    28501 gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc accgatcgat
    28561 gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact gaaatcatag
    28621 ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac cgacttgctt
    28681 ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat acttaatact
    28741 tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc atctgtgacc
    28801 atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt gtcacctgta
    28861 gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc agatggtgtg
    28921 aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt ctcaaccgtt
    28981 actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt ttgaattttt
    29041 agcggaacct catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac gcggaacatc
    29101 cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga aagtttgaca
    29161 gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga aatcttgatt
    29221 ggcaccccag taactgtttg attatacgct tcatttccag aaagcgtatt tggaacggac
    29281 tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa actgtaaaga
    29341 atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc ataccgtcta
    29401 ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatc agttctctac
    29461 gcaaaattgc caaatattct tgctgggata agccagccat cagttcatta tctaatgtaa
    29521 tgctgacatc cccagtttct tcagagacaa cgatggtaat cgcgtcgctg acttcgctta
    29581 taccgacagc tgcccggtgt cgagtcccga attcttttgg aatcaacata ctttctgata
    29641 gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact gcaccgtcat
    29701 gcagcggtgt attaggaata aagatattga tcagcagctc tccagtgata tctgcatcta
    29761 atgcaattcc tgtctcaata tactcatcta atccagtatg ccgttcgata gtaattagcg
    29821 caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag atcatcttct
    29881 catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca agatgttcca
    29941 gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg tacgtaatga
    30001 cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg attcggataa
    30061 caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc agcagttgga
    30121 tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc aaaaaattca
    30181 gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa agttccccga
    30241 tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag tatatcatat
    30301 gttttaattt tagaggctga tctgctcgta tttttcttga gaaaaacgca taattttcag
    30361 gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa cttcagagaa
    30421 ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt gttccatttt
    30481 tttacgtcgt tctgctctgg cagctcttct ttcttctgct tcaaggtccg tcgttttttc
    30541 ttttctagtc ttaacgggta catcgataga ttcctctggt tcaagtcgtt tcttgatttt
    30601 ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt cttggatagt
    30661 cttttcaaat aaagcttttg caagatttgg atttgcgttc tctagcgagg aaaagcgaac
    30721 ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt tttttgtagt ccaaagtcat
    30781 tgggttcttg tttttttcga ccattgcagg gttatatcga tataatgacc aatagccaga
    30841 agaaacagct tcttttgctt ctttaagcga ttctttcatt ccaccaagca aaccatgtgt
    30901 gatacaaggc gtataggcaa tgattaaaga aggtccagga tacttttctg cttcttcaaa
    30961 agcctttatc gtctgcattt gattggcacc tgaagcaatt tgcgccacat aaacatttcc
    31021 atatgtcatc gccatcattc ctagatcttt ctttgaagca tactttccac tagccgaaaa
    31081 tttagcgatt gccgaagctg gtgttgcttt tgaggtttga ccgccagtat tggaatagac
    31141 ttcgttatcc aaaacaagaa tattcacgtc tgctccgctg gcaagtacat gatcgatccc
    31201 gccaaaacca atgtcgtaag cccatccgtc tccaccaatc atccattggc tgttttttac
    31261 gaatagatcc tttctcttgt atatatcctc cagttcaggg acaattgatc gttcgctttc
    31321 gatcgcagct tgcagttttg ctgctctttg ctgcgttcct tcgctctcaa acatgtgtac
    31381 caaccagtct tccatcaatt ctcgtagttc ttctgatact tttggcaata cttctttgat
    31441 tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga agcataccat aaccaaattc
    31501 agcattatct tccaataaag aatttgacca tgcaggccct tgaccacatt cattggtcgt
    31561 ataaggagaa gcaggagaag caccgcccca aatagaagaa cagcctgtcg cattcgcgat
    31621 catcattcga tcaccgaaca tttgtgtcaa taattttaca taaggtgttt cgccacagcc
    31681 tgagcaagcg cccgagaatt caagaagcgg ttgattgaat tgtgtagaca agacagtatt
    31741 ctttcctttg actggatttg ctttttgtct taatgtcatt gcaaaagccc aattcacagc
    31801 ttgttctttc tgttcttcat aaggcttcat cactaaagct ttttcttttg cgggacaagc
    31861 ctctacacac agaccacaac ctgtacagtc ttccactgaa acttggatac ggtattttac
    31921 gccgtctgcg cctcgcattt ctctcacgat aaatccttct ggcgcttctt ccatttcctc
    31981 ttcatctgct aaaaatggac ggatggcagc atgaggacaa acaaaggcac attcattaca
    32041 catcgtacag cgatcactaa tccactcagg tacttctaaa gcgacgcccc gcttttccat
    32101 agaagccgtt cctaaaggca tttcgccagt tgtcattcca tttgctacaa gtgtaccaac
    32161 agagagttga tttccttcct gtgcattcac tggttcaagt atttcttgta tataaccagt
    32221 gttcctttta ttgatttttt caggcatctc caacgttctc caatggtctg ggatcgcgac
    32281 ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg tttttttcta cgatagccat
    32341 tgatttatgt ccatagcttt tcagggcttc ttcttttagc agcggaagca cctcatcaaa
    32401 aggaatgatg tcagctaatt taaaaaaagc cgtttccatt gccgtattga tccggcgtcc
    32461 caaaccgact tcttgggcca atctcatggc attgatcgta tagaatctaa tgttattttc
    32521 agccaagtaa cgttttaatt ttaagggcag atgcgtttct aattgttcgt cgctccaaag
    32581 agtattcaat aaaaaaatgc cacctggctt caatcctttg accaagtcat agctatgcag
    32641 gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg acaagatacg ctgaacgtat
    32701 cggcgtatct ccaaaacgaa gatgagagac agtcagaccg ccagattttt tcgaatcata
    32761 ataaaaatag ccttgcgcat atttatcggt gtgatctcca ataatcttga tagctgattt
    32821 atttgctcca actgttccgt cagatccaaa tccccaaaac ttggcctgga atgtttgcgg
    32881 ttctgtcaaa tccagacttt cctttggttc caaagattga taagtcacat catccacgat
    32941 ccctatggta aaccgttttt tgcgtatgct tgggtctttt ttcaattcat caaaaactgc
    33001 tgagatttgg tcgggagtca catctttaga ccccaatccg tagcgaccac caataacagc
    33061 cgggcgaagt tcagaatcat ataacgcact ttgaacgtct aataagagag gttctccacc
    33121 tgcccctggt tctttacttc gatcaagaac agcaattgtt tttactgtat gaggcaactt
    33181 ttgcacgaat gtttccactg gaaaaggacg ataaagatgg atattcaaaa aacctgtttt
    33241 ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa ctgatcccat
    33301 agaaacaatg acttcttccg catcgtctgc tccgtaataa gtcaccaagt cataagaagt
    33361 tcctcgtaat cggttgatct ctttcatata tttttgaacg atttcaggta atttatcata
    33421 ataggggttg atcgtttccc gttgttgaaa atggatgtct ggattctggt tcgtaccact
    33481 aacagtcgga tgattgggat tcatccctcg tttacgaaat gaagctaatt tctcctgatt
    33541 gatcagagga gctaattctt catattccaa aacttcgatt ttttgaatct cgtgactcgt
    33601 acggaatcca tcaaagaaat tcaaaaaagg tacttctcct tcaatagaag caagatgggc
    33661 aacaggtgct aaatccatca cttcttgtac actgctttca gctaacatag caaatcctgt
    33721 ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat ttgtcgtcac
    33781 tgcccgacta gcaacatgga aaacacttgg cagtagttcc ccagcaatct tgtacatatt
    33841 cggtatcatc aaaagcagcc cttgagaagc ggtatatgta gttgtcagtg caccagtttt
    33901 caatgatcca tggaccacac cagctgctcc tgcttcggat tgcatttcta ctacttttac
    33961 tggttgaccg aatacatttt cttttcctgc tgctgaccat tgatctacta attcagccat
    34021 ggtcgaactg ggtgttattg gatatattgc agcaatctcg gtaaatgcat aagaaatata
    34081 tgcggctgcc gtattgccat ccattgtttt catttttcgc atgattcatc atccttgttc
    34141 atattctctc ttattatctg ttccattata acgtaaaaca ttccattttt tcttttattt
    34201 ataaaaatat ttcacatatg tctatttgaa ttggtcatct aatttttaga caaacagtct
    34261 aaatcgttgc ctttttttgt cagacatgta taaactataa gcgaaagagg tgttttaaca
    34321 tggatatatc agtgattgat gcaacgaaaa caaatacaca aaaaggaatc ctttatggtt
    34381 catcgaatgc tcctaaaaaa atggtcgaat tcatcaacct atcttgtccg tattgccgac
    34441 aatggtttga agaatcttat gagctattag aagaagcagt ccaaagcggg caattgcagc
    34501 gagtgatcaa attgttcgat aaagaaaaag aaagcttgca gcgaggaaac gtgatgcacc
    34561 ggtatctcac aatcagcgat ggtcaaaaag cgatcaaaga gatcaaacaa atattcgata
    34621 ctcaagacga gtggaaacat ctttctctcc aagaagtagc ggattttgca gtagataaac
    34681 taaagctgac agaacaaaaa gacgaacagc tgagccaagc tgtcatcaac gaagctgagc
    34741 aagcacatat tcgatttgtt ccgactgtca tcttgggtaa agaaatcttt gatgaatcta
    34801 tttcaataga ggaattaaaa gaattgatcc aaacaaaata aaaaaataag gagcgtagga
    34861 caaaagtaaa gaacactttt gtctcacgct ctaaacacct ataaacggcg gaagcagaag
    34921 caactccttc ttatttcttg gagttaaacg cttctgtccc agcctcctta ttccgaataa
    34981 atggtgtcca aaagcttgca tctgccagat tatctcattt cttatgacaa gctgagcaaa
    35041 ttatctccac caaataattt attcatattt ttgacttgcg catactaact aattcttaaa
    35101 ttttcataca aaatttctca aattccctta acgagtaata tttatgtacg gtgattcaca
    35161 ctagtatccc aatcactttt taagttatcc cgatttcaat tctacttcaa aaatagttgc
    35221 ttttatgtat gctgttagtt gattagatgg tcgtttgttg agctaggtcg tttattttaa
    35281 acaagttgtt tctttagctt atacctatct cgtctaccaa acgcaagatg gcaaacacaa
    35341 atgcatacgc atctgtattt gccactttga ttcgtataaa gctaacaact cctaactcct
    35401 ctcatttaat agttgctctc ttttttattc agatacattt ttgctgatca cagcctagta
    35461 tttcaatggt tttgaacact aaaccaaatt gttccattac aacattcact ggctgatgaa
    35521 atggattaat agatttcgat tattcaaagc gattttctat tggtggattg tgttcccaac
    35581 taacaagacg gattccaaag tcattatata actcttcaag agttaaccct ttcctgttcc
    35641 caatagatgt atctctccca cgctcgatat aaaatatgga gtttcgaatg ccaagacctg
    35701 gcaaatcttt ccttgttaca gttacactgc tactaacttc cccatataaa atccagctcc
    35761 tccaaaatga taatatattt ttcgaccatc atctaactca tgtaacaata attgttgtgc
    35821 cctcttgtca ctcgccaaat gcaaacgcca cgtgcgatac catgccatgc tacctgcatc
    35881 tgcagaaaat ccatcaaata atgcaacaat tgtatcttct atcacaatcc tttccccatc
    35941 tacttcatat tctaatctaa aatcaaactc ccctcgggta atcgcaggtc ttggtggctt
    36001 aggacccaat attattctac ttaaaaccgc attcgttccc agatgaaaca cagctataac
    36061 aacaataatg attaatattt tgtttctatt tgataaattt ctcattattc gcaaatcacc
    36121 tctacattct cacatactag ttcgtaatac tcctttaata tcaaaatatc tactgatcgt
    36181 aataatattc gctatgaatt cctactactc gtataagaaa ggccaaatca tcagctttta
    36241 cttgctgatg atttggcttg tttctattta tcgtaattgt tttctaatca ccaaaattgt
    36301 taacaatcgg ttcagatatt tcccaattca ctagccttat attatattga tttaacaatt
    36361 cttcttcatt tattgtattt tgatcaagcg gaccaaattg ttctaataat tctttgtcat
    36421 gtaaaatatc ccttctagta atgcctcttt ccctttcaat gaaagaagca ctaggaaaag
    36481 tatgattata accattcaac ctatctccca tgtaataatt agcaggtcct ggaaaataaa
    36541 atatttgctt attatcacta acttctaaca gcaacactaa atcttctccg ctggctaaac
    36601 gacttgtcca tctacgatat ctccctatgc cttcgttcat tccaaatcca tcaaattcaa
    36661 ctattaacgt gtcttctatc acatgtaatt cttcattcaa ttcatactca agtctaaaag
    36721 gaaattctcc atatgtaatc tctggcaatg gtggtgctgg agttctccaa ataaatacaa
    36781 taactgtgaa tagaaaaaat ccaacgatcg atataaatgt tatgagacct gctcgaagta
    36841 aaacccgata taataaaaat gctgtatttc gctttgctat atctagaaaa ataaaaagta
    36901 ctgcaatcat aatgatagta aaagttgtaa aaaaacgcat ttttgccctc cctttataaa
    36961 catcgattct caaattctaa accaaatctt ggtagttcaa gttcaggtat aacgtattcc
    37021 aaccatacat ctcaattact atttatgaac ttatgattat cactgaataa atcactccat
    37081 ccccttgtta aaacttctac attaattaca cctggttgtt ttcgactagc taatccaatg
    37141 aacttatcaa ccatccttat ccaccaacca cgagaatctt gaccagaagt aagaagagca
    37201 gttctcaagg atgatcgagc agttccttcc agttttttaa ttaatcaggc cagttataga
    37261 taaatacttg atcttatcta tatcaggact tctatattaa gcaaaaattt atgtacaaaa
    37321 ctaaaatctt cattcagttg tctccccgct ttttatacga ttgtattaaa aacaatagaa
    37381 gcctttccaa attatgttac tccagtaaag atggaattat agtttgacgg aagaaaagca
    37441 agtataactt ttaaaaaaaa ctgatacatt cgcatctcct gcttcatgta tcagtttttt
    37501 ctttggatta aactgcttct tcacgcagtt tttccgcaaa ttcatttatt ttgcgaattc
    37561 ggtgattaat tcccgatttt gatatcgcac ctgagggaat catttcccct aattctttca
    37621 agctgacctc tggatgttga agccgaagtt ctgcgatttc ctgaagtttt tcaggaaggg
    37681 catgtagacc gacctttgct tcgatcaatt cgatgttttc gatttgttta gaagccgcat
    37741 caatcgtctt gttcagattt gctgtctcac aattgactag acgattgacc gaatttctca
    37801 tatcccgtac gattcgaaca tcttcaaatc ggagcattga gttggttgca ccaatcaatg
    37861 ttaagaaatc agcaattttt tctgctcctt ttaaataaga aatatagcca ttgcgccgtt
    37921 caagcgttcg agcatgtaga ccgtaataat ttaacatatc gcaaatgtct tggttatgct
    37981 gttcataaat agaaaatatt tccaaatgat aacggctcgt ttccggatta ttgactgaac
    38041 ctgaggccat aaaagcacca cgtaagtaag aacgcatttt ttgcgaattt cccataatct
    38101 cgtccgaaac gtgtccattg aacatcatac catccatgat atcaagatcc atcaacacat
    38161 cttttgtgcc ttgcttcaac cggacgatat agacattgtt cttttttaat ttcatttttt
    38221 tacgaacgag tagctcactt cgtacctgat aatgatcttt caaaagagaa tagatacgtc
    38281 tggcaattgc agcattttct gtttggacgt tcaaaataaa ctgctggttc acaataccta
    38341 aagagccgtt cattcgcagc aaagcagcta attccgcttt cgcatgttct cgatgaactt
    38401 ccaaaccagt tagttctttc ttcacatctg cagcaaatga catcctattc tcctcctttc
    38461 tccaacaata aattgcttaa tattttgaac cgaaaactaa acggaacaat tcttctacta
    38521 ctttgtctcc atcgtggaag acaccaccat ctttcaattc caaaaagtcg gtggaaatca
    38581 ctcgacatcc ttcatttctc aatccgttga agtcgtgtgc gacttgtact agatactcat
    38641 catagatctc tgggtccata taatcttcag gcactttttc cgtattgacc aatactgtat
    38701 cgataaaagg ctgattcaaa tgcttgtgta aaacacgtat atgatcggca tctgtgaaat
    38761 gttccgtttc ccctttttgg gtcatgatat tacagatata gacggtttca gcagttgtct
    38821 tgagcattgc ttcgccgatt tctggtatca ctaaatttgg aaggatactg gtgaataaac
    38881 ttccgggtcc caaaacgacc atgtcggcgt ccataattgc tgaaacgact tttcgagccg
    38941 ctttggcttc acttttatca tttgtgttgc agacatacac acggtcaatg gttttacggt
    39001 cgatcgcgat tttagattct cctactgcag tcgttccgtc tttaaagaca gcatgcaagg
    39061 tcaaaggagt ttctgaagaa ggataaacat gtccatctac atgcatcatt tttgctaata
    39121 attgaatcgc ctcataagtg cttccccgca tctcggaaac tgcggcaatg atcagattac
    39181 cgatcgtatg attggctaaa aactgatctt cctttttgaa acggtattgg aaaatatctt
    39241 catagaattg tggcatatcc gataacgcca ctaagacatt tctcaagtca ccaggaggag
    39301 ccatagcgat cgattctctg attgcaccgc tgcttccccc atcatctgca acagtcacta
    39361 ctgctgtgat atctgctcct tgatttcgca aacttttcag gataactggc agaccagtgc
    39421 caccgccgac cactacgatc tttggttttc ttatccggta ggttttcatt ttcacgaccg
    39481 gttcaccgtc tctttccttt tatccttgtc ccgatgtgtc acattgactt tatagttttt
    39541 ccctaaagca tttgccaaac gttcagtcaa tgccacagaa cggtgttgac cgcctgtaca
    39601 gccgattgca actgtcaagc tgctttttcc ttctttaatg tatccaggca atacagtttc
    39661 cagaagatcc aaaaatttat ggtagaatga ttgcgtttca tcaaaggaca tcacataatc
    39721 atagactggc tgatcttttc cggtcaatgg tcttagttcc gggatataat gagggttggg
    39781 caaaaaacgc acatccatca caatatccga atcgatcggc aacccgtatt taaaaccaaa
    39841 tgatacaaat tcgatccgaa atccttggtc tccctttgat ttgaatgact cattgatttt
    39901 ttcacgtaat tgtcgtggtg tcaatgtcgt cgtatcaatg atcacggaag cttgggcttt
    39961 caagtcgtct agtatcgccc gttctttacg tatcccttca gtgatcaaac cgtccatcgc
    40021 cattggatgt gctcgtcttg tttctttgta gcgagccact agttcttcgt ctgaagcatc
    40081 taaaaataag atagaagtgt cgatgaagtt cgtattttcg atttcgatca gcatgttttg
    40141 gatttcttca aagaaggaac gagaacgtaa atccactcct aaagccattt ttgtgacttt
    40201 gcctgattct ttgattaatt cccagaattt tggaatcaat cgaggcggca tattatcgat
    40261 acaaaaatag cccatatctt caaaactttg gatagctact gttttccctg caccgctcat
    40321 acctgttatg atgactaatt gcaaattatc tgtcataatt acgaactcct ctcacattgt
    40381 gcatagtaaa attatatcat tccgggcgta tcattaccag ttttcttttt cttttacaaa
    40441 aagatttgga ccaaatatct tttaggatat ttgccccaaa tccaattttt ttacttttcc
    40501 gatgctgtat ccaatgcctt gcttctttca gctagcgctc gttcaatcgt ccgatctgct
    40561 aaaatagatt gggcatcgat cactggataa tggttatctt cagcaaattc atgcatcaat
    40621 gacaattctg tgcaacctaa gataactttt tcacagttta ggcgctctac cgcttcttct
    40681 aagatttcgt aatatagctc ttgattcaaa taatccgatt ccttgatctc atgatagatc
    40741 agataattga ttttttcttg aagaacagta tcaggaatca ctgtttcgaa tccaagattt
    40801 ttgacttcgc gttcatagat tccagctttc atacttcctt ctgttcctag aatcgctact
    40861 ctccctgtcg tatgctgacg gacaagttca tttgctgctt ctcttggcat atgaaggata
    40921 ggaatatccg ttgccgcttg taattcttca aaaaagtagt gtgcggtgtt acaagtcaac
    40981 acaatgaagt tcggctgcaa taaattttgt ttctcaatat catccagcag aaacggcatt
    41041 ggattttctt ctgatcgatc taaaatataa gctgtacgat caggaactgt tgcatgattg
    41101 aataagacat aattcaaata ttcttgatct tttgtagctt tcgttcggtg gttgatcaag
    41161 cgaacaaaac tttctgtcgc catcgtgccc attccgccta aaatactgaa aaaattctcc
    41221 atgaactagc cttctttcac atgaaaatac tttttgaatc ttggaatata gttatggaac
    41281 atatatttca tcaacaacca gcgcttgatt gaccggtctt tttcataaaa cacagtagta
    41341 ccgtaacgat tttctttgat catttgctca gctagttttt tgtcttcatt ttcccgagca
    41401 tattctaaaa agattttttt gggcacaccc atccataatc ttgctttatc tgattgattc
    41461 gttccatagg tcgtttcaac aaagggtttg ttaaacactc gatcttctgt gacgaaacga
    41521 gccagattca agccgttcaa tgtcacaaag aaactgctac gaccttgtct caaattgatt
    41581 tcaaataatt tatattctcc atctctagga tcatacttca tatcgaaatt ggcaaagcct
    41641 gtatattcga ttttttccaa aaatgcctta atggtttgat agattttttc gttataatct
    41701 ggcatgatca caacgtaatt accaatcgat gctggtgtag gatcttctaa cagtggatga
    41761 ccaagacaca tcatccgaac ttgatgatct tcatctacat acgcatttag cacacgcata
    41821 ttactatcat cacccgggat aaaatcttgt acaatcattt cacttttata accggcttcg
    41881 tatatccggc ctaaaattaa atcgaactct tctctagtct ctaaaataaa agcttttttt
    41941 cttccttcaa attggacaga aagatactcc acactgtttg caggtttcaa agcaactggg
    42001 aattcaaaag gcaattcctg ttccaaatgt ccatttacta acatttcttc tctgacgatc
    42061 aatgttttag gatacggaag atcatattct tcacaaactt cataaaagct tactttattg
    42121 atcagtcttt caaataaaga ataatcaata tatggacaga taaaatattc tgacagctct
    42181 tgtttatgtt gggagatcaa ttctgcatat ccatcaccac aagcaatcaa aagatatttt
    42241 ttactcttat cagaatatct ttcttttgcc aaacgaagca tcgtctcgat aaacacaggg
    42301 tctttatcaa atcctggaat aacttctaca ttgacaattt tactgtagcg ggtaggggct
    42361 aactgatccg aagcatatgc ttggcagatt ttgccgtatg cttcattaaa agaacgtgcc
    42421 ataccataaa cgttcatgtc gcttcctaat aatataggga taaattcttc attttcaata
    42481 ctgttcatca ttatctctca cttactcata atctcttgtt tttcttttcc aactacacaa
    42541 ggaatatcat accataaaga aaaaagcaat caaaggaaac tcctccgatt gctcaattct
    42601 tttttgaacg gattgaactt ataggaagtt tttgtctata ccaatcagtg tagaaagaac
    42661 taccatgtaa tgattctaga acactgatat taataaaaca agcaaaaatt cattttttta
    42721 tgttttttta actatctcag ctatctatcc tctttatacc attatttttt tcaaatagtg
    42781 acctgtataa ctttctttta cgttaatgat ttcttctggt gttccagtcg caaggatcgt
    42841 tcctccacct tctccacctt ctggtcctaa atcaatgata taatctgctg ttttgattac
    42901 atctagattg tgttcaatca ctaaaactgt gttaccagca tctactaatc tttgtaatac
    42961 atgcaacaag cgggcgatgt catcgctatg aagtcctgtc gttggttcat ctagtatata
    43021 gaaattcttt ccattagaga ttttgtgcaa ttcactggca agtttcatcc gttgtgcctc
    43081 accaccggac aatgtcgttg ctggttgccc catcgtcaca taacctaagc caacatcaac
    43141 aatcgtttgc agtttgcgat gaatctttgg aatgtgcttg aagaattcta cagcatcttc
    43201 tactgtcatt tccaaaatat cagcaatgct ttttcctttg taatgcactt ctaatgtttc
    43261 agagttatat cgtttgccat gacatacttc acaaggaaca tagacatcag gcaaaaagtg
    43321 catttcgatc ttaataattc catccccgcg acaagcttca caacgaccgc cttttacgtt
    43381 gaaactaaaa cgccctttct tataaccccg catttttgcc tcgttcgttt gagcaaataa
    43441 cccacggata tcatcaaata cacttgtata agtcgctgga ttacttctcg gcgtccggcc
    43501 gattgggctt tgatcgatat cgatgatctt ttcgatactt tcgtagccgg aaattgtctt
    43561 gaatttacct ggcttagcag aattcttatt taatttttgc gctaacgatt tctttaagat
    43621 actattgacc aatgtactct ttccagaacc agacacacct gtgactgcta caaattctcc
    43681 taatggaaat tcaacactaa tatttttcag attgttttct gaagcaccag tgatcttgat
    43741 tgctttccca ttacctttac ggcgttcttt tggtacaggg attgattttt tccctgaaag
    43801 atatttccct gtcaaagaat tttcatcatt agctacttct tcaggagtgc ctgcagccac
    43861 gatttgtccg ccttgttctc cggccccagg tccgacgtca atcagataat ctgctgccca
    43921 catcgtatct tcatcatgtt cgactacaac gagggtgttg ccaagatcac gcattttctt
    43981 taatgagcca atcagtcgat cgttgtcgcg ttgatgcaat ccgatcgatg gttcatctaa
    44041 aatatacaaa actccagaaa gattcgatcc aatttgtgta gccaatctga ttcgctgcgc
    44101 ttctccccca gataatgttc cggaagcgcg actcaaagtt agatagtcta gtcccacatt
    44161 ttccaagaat gaaagtctgt catttacttc ttttaaaatc ggtttggcaa ttactttctc
    44221 ttgttcagat aacgttaagt ttgagaagaa atctactgct ttttgaatag acaactcatt
    44281 tgtttgcccg atattcgttc cattgatttg aacagataga gcttggggat tcaatcgata
    44341 acctttacat gtctgacaag ttaattctgt catataaaga cgcatttgct cacgcgtgaa
    44401 atcactgttc gtatcatgat aacgacgctc gatattcttc aatacccctt cgaaagtcgt
    44461 ttctacatcg cgaacgccac caaaatcatt ttcataatgg aaatggaaaa gatcatctgt
    44521 cccattcaag atgatatcct gatgctcttc cggtaattct tcgaatggtg tatctaaatc
    44581 gataccgaaa ctttcacaag cctgcgctaa catttgagga taatactgag agctgattgg
    44641 attccatgga acgatagcac cttgactaag tgtcttcgtt ctatctggaa tcactaaatc
    44701 ttgatcgact tctaatttga cacctaaacc atcacagtct ggacaagctc caaaaggcgc
    44761 attgaatgag aacagtcttg gttctagttc gccaacagta aatccgcaat aaggacaggc
    44821 atagtgttcg ctaaacaaca tttcttcttc accgatcacg tcaaccagag catagccgtc
    44881 tgccaatcgc agagccgcct ctaaagaatc aaatagacga gaacgaatcc cttctttcac
    44941 aatgatccgg tcgatgacga tcgcgatatc atgttttttg tttttctcca gctcaggcac
    45001 ttcacttaaa tcatagatct caccatcgac tcgaacgcgg acgtatccct ctttttggat
    45061 tcgttcgaat atttttttat gttgtccttt tttctttacc acgatcggtg ccaacacttg
    45121 gatctttgtg cgctcaggca aagccattac ttgatctgcc atttgatcta ctgattgact
    45181 ggtaatctcg atatgatcgt tgggacaaat cggatgtcct acacgtgcaa acaacaagcg
    45241 taaatagtca ttgatttccg tcacagtacc tactgtcgac cgaggatttt tacttgttgt
    45301 tttctgatca atagaaatcg ccggactcaa tccgtcgatg ctatctacgt ccggcttatc
    45361 catttgtcct aaaaactgac gagcataagc agaaaggctc tctacgtatc tccgctgccc
    45421 ttcagcgtac agcgtatcaa atgctaggga actttttcca gaaccggaca aacctgtaac
    45481 gaccacgaat ttatctcgtg gaatcgttac atctatattt ttcaagttat gggcacgtgc
    45541 accgtgaatc gtaattttat cgttagccat gttgtcctcc tactttgaag cttttaattc
    45601 taaaatcata tctcgcacat tagcggctgt ttcaaaatcc aatgctttcg ccgcattttt
    45661 catttctttt tctaatttca tcaataacgt atctttttct tctttcgtca tttcttcata
    45721 cgagacttgt accgcttctt ttgtatcatc ttccgattct ttcgtaatag aaatcaaatc
    45781 tcggatttct ttaatgatcg tttttggaac gatcccatgt tcttcattgt acttctgctg
    45841 aatcgtacgg cgtcttgatg tttcatccat cgctagacgc atagaatctg ttactttgtc
    45901 tgcgtacatg attactttac cttcttcatt ccgagcagct cgtccaatcg tttggactaa
    45961 ggaacgttca ctacgtaaaa agccttcttt atccgcatcc aagatagcaa caagggaaac
    46021 ttcaggtacg tctaatcctt cacgaagcaa attgattcca actaacacat caaattcacc
    46081 taatcgaaga tctcgaatga tttctgttcg ttccagcgtt ttgatatcac tatgcaaata
    46141 tttgactttg atccctaatt ctttgaaata atctgtgagg tcttctgcca tttttttcgt
    46201 caatgtcgtt acaaacaccc gctggtcttt ttcgactcgt tcatttattt cgcctactag
    46261 atcatcgatt tgtcccatga ttggacgaac ttcgatcact gggtcaagca gccctgtcgg
    46321 acggatgatt tgttggataa cggtatctgt ttgttcatgt tcatatggtc ctggcgtcgc
    46381 agagacataa atgatctgat tgacatgttt ttcaaactct tctaaccgaa gtggccgatt
    46441 gtctaaagca gatggcaaac ggaaaccgta atctactagc atctgttttc tcgctcggtc
    46501 accattatac attcctctga cttgaggcat ggttacgtgc gattcgtccg ccacgatcaa
    46561 aaaatcttca ggaaagaaat ccaatagcgt ataaggcggt tcaccttctt tacgtccgtc
    46621 catatgccga gaataatttt caattcccga cgtgtagccc atttctaaca tcatttcaat
    46681 atcgtaattg gttcgctgtt ctaaacgttg agcttccaga agtttgtttt catttcgcaa
    46741 gacagttaaa cgctgttcta attccgcttt gatattcgct acggcatgct ccatatgttc
    46801 atcattcgtc acaaagtgag tcgccgggaa gattgccacg tgttccgttt ctcctaaaac
    46861 ttcacctgtc agtgcattga cttctcggat acgttcgacc tcatctccaa aaaactccac
    46921 acgtaaggca cgttcatctc ttgaagccgg aaaaatctcc acgacatcac cgcgcactcg
    46981 aaaacgaccg cgctgaaaat ctatatcatt tctttcgaat tggatactga ctaagtcacg
    47041 aatcaactga ttgcgatcta gttctgctcc ctgtcgaatc gaaactactt gtttttggta
    47101 ctcaaaaggc gaacctaatc caaaaataca tgaaacggat gcaatgacga tcacatcatt
    47161 tctttctaat aatgaactag tagccgaatg tcgtaatttg tcgatctcat catttacact
    47221 tgaatctttt tcgatatacg tatcgcttga aggcacataa gcttcaggct gataataatc
    47281 ataataagaa acaaaatatt caacagcatt gttagggaaa aattccttga attccccgta
    47341 cagctgacca gccaatgttt tgttatgggc aataattagt gttggtttat tcactttctc
    47401 aataagattg gaaatcgtat atgttttccc tgttccagtc gcacctaata agatttgtgc
    47461 ttttttcccg ccgactacgc catccactaa ttggttaatc gcttcaggct gatcaccagc
    47521 cggttggtat ttcgagacaa gctcaaagtg tcgggaggta tctctctcaa tcattcttcg
    47581 tcctcctttt cattcattcc ttttcatttt acgaccaata aaaagaaaag gaaagcaaag
    47641 gcattcctta ctaaaaaatt ataccacacc gaacatactt tcgccacttt tttatcttat
    47701 tatgcatgtt acgttttata acataaaatg ttagaaactc gcattttttt cattttttcg
    47761 agcttacagt cttgtctgat tcagcacttt tcgcctataa taataaaata ttggaaattt
    47821 aggaggttga ttatgaaaag aaagcattta tttcactcat ttgttttctt aatgacagga
    47881 cttgtgacac ttgcgtttgg acatgttgca caagctgaca acactacaac ggcagagtca
    47941 agcacaagcg agacaatgca aaaaatagag cccaaaaaag acgtatacgt aattgccagt
    48001 gactctgctt ttgctccatt tgaatttcaa aacgcaaagg gcgagtacga aggcatcgat
    48061 gtaaaattga tggaaaacat cgccaaactg caaggattta acatccgaat ggactaccgt
    48121 ggtttctctg ctgctttgca agctttggaa tcaggacaag cagacggaat gattgccgga
    48181 atgactgtaa cagatgaacg aaagaaaagc tatgattttt ctgattctta ctttgatagc
    48241 gggatccaat tagctgtcaa aaaaggagat acttcgatca agtcttatga agatttgaaa
    48301 ggaaaaacag tcggagcaaa agttggtacc gaaagtgctg aatttttaag cgaccatgaa
    48361 tctgaatatg gctattctat caaatatttt gatgctgctg atcaattgta cgacgggtta
    48421 aaagttggcg caatcgatgc aatgatggat gattatccgg taatcggtta tgcgatcaaa
    48481 caaggacaag aattggaaac accgatcaaa aaagaatctg gcggaaaata tgcatttgcc
    48541 gttaaaaaag gacaaaatcc tgagcttctt aatatgttca atgaaggatt gaaagaatta
    48601 aaaagaacag gcgaatacga taagattatt aaagaatatg tagcggatgg ttcagaaact
    48661 tccagtgagt cttccactgc tgatgaatca acatttcttg gacttatcca aaacaactat
    48721 caagcattgt tgaaaggact ttggagaaca attctcctaa ctttgatctc ctttgctttg
    48781 gctatagtaa tcggtgtgat ttttggatta ttcagtgtag caccaatacg cgctcttcgc
    48841 acaattgcta gtatttatgt cgatatcatc cgaggaattc caatgatggt tcttgcgttc
    48901 tttattttct ttgggttgcc tggtatcgtt ggatttacga ttcctgactt ccttgctggt
    48961 gttatcacct tgactcttaa cgcaagtgct tatatcgcag agattgttcg aggcgggatc
    49021 aacgccgttc ctgtcggaca aatggaagct tccagaagtt taggtctttc ttataatcgg
    49081 acaatgcaaa aaatcatttt gccgcaagcg atacgcatca tgatcccatc attcgtcaat
    49141 cagttcgtta tctcattaaa agacacaacg atcatttccg caatcggtgt agtcgaattg
    49201 ctgcaaactg gtaagatcat tgttgcacgt acaacacaaa gtacgtatgt ttacttgatc
    49261 attgcgatca tgtacttgat tttgatcaca gcattgacaa aattagcaaa agttttagaa
    49321 aagaaggtga agtaagatgg cagaaaaaat cttagttgag catttagtca aaaaatacgg
    49381 cgataacaca gtattaaacg acatcaatgt gtccatcaac gaaggagatg tcgtctgtat
    49441 cattggccct tcaggttcag gaaaaagtac atttttgcgt tgcttgaatc gtttagaaga
    49501 accttctgac ggtgaaatta ttattgacgg cgcccacttg atggataaaa gcacagatat
    49561 caacttggtg agacaacata tcggaatggt attccagcac tttaacctat tccctcatct
    49621 ttctattttg gaaaacatca ttttagcacc gatggacctg aagaaagaat ctagagaaga
    49681 agcagagaaa aaagcaatca aattgctgca gacagttggt ctagaagata aaaaagatat
    49741 gtatcctgaa atgctttccg gtggacaaaa gcagcgtgtg gctattgctc gtgctctagc
    49801 gatgaaccct gacatcatgc tttttgatga gcctacatct gcactcgacc ccgaaatggt
    49861 cggtgatgtc ttgaacgtca tgaaaaagtt agcagaacaa ggtatgacga tggttatcgt
    49921 tacgcacgaa atgggcttcg ctaaagaagt ggccaatcgt gtcatgttca ttgacgacgg
    49981 aaacttttta gaagacggaa cgcctcaaca aatctttgaa aatccgcaaa atgaacggac
    50041 aaaagatttc ttgaataaag tattaaatat ttaataaaaa ccaaaaatgc ccggtcgctt
    50101 catgacgacc gggcattttt agtttcttgt tttttcactt aactgtacca gccgcatcgc
    50161 ttgttctcct gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat
    50221 cggttcattg acgatatcaa gtacatagtc gataccacct tcagaaacta tgatgattat
    50281 cttattctaa agctggcttt tttgttggta caaacatgac aactacaaaa aaaccaggcg
    50341 aacgtatgat tgttcgtctg gtttttaatt attaatgact tagcgttttt actagaaata
    50401 gaaaatttgg gcaagtctac tttatatttt tactagaaaa actacttccg ctttttcccg
    50461 ccaaaagcat ctttaaactt atcgaagaaa ccgtcttctt gctgttcttc aactttgatg
    50521 ccgccagctt cagcgaatgc acgtaatgct tctctttgtt gttcatttag atttttcggt
    50581 gtgatcagtt ttactttgac atgttggtcg cctgttgcac caccacgtaa cttcggagcg
    50641 ccttttccac gtaaacggaa gttcgtacct gtctgtgttc cagcagggat cttcaatttc
    50701 acatctccat ggacagttgg tacttggact tcatccccca aagctgcttg tacaaaactt
    50761 aaaggcaatt catagtaaat ttcagaacct tcacgatcaa agatatcact ttcttctaca
    50821 cgaaaaacca catacaaatc accaaatggt ccgccgtttt ctccagcttc tccttgacca
    50881 gctaaacgca tttgttgtcc ttcttctaca ccagctggta cattcacttt aactgaatgt
    50941 gctttcttct catgtcctga gccatgacag gttggacaag gttctttgat ttctttccct
    51001 gtgccatgac aaacatcaca agtttgacgg ctcatcactc gtccaagtgg cgtttggcgt
    51061 tctacattga tcgtacctgc accatgacat ttatgacaag tagttggttg tgtgcctggt
    51121 tttgcaccgt ttccaccaca agtatgacag atttcttccc gattatactt gatttctttt
    51181 tctacaccaa aaatcgcttc ttcaaaagac aagtcgaccg tatattgcaa atcagcacct
    51241 tgacgaggcg catttggatc gactgaccga ccgcctcctc caaagaaaga gtcgaagata
    51301 tcttcaaaac caccgaagcc gccaccagag aagccgccga atccaccaaa tccgccacca
    51361 gctccaccag caccgtaatt tggatctgtg cctgcatgcc cgtattggtc gtatgcagcg
    51421 cgtttttgcg ggtcacttaa aatctcatat gcttctgata cttctttgaa tttttcttcc
    51481 gcatcaggtt ctttattgat gtcaggatgg tattttttcg aaagcttacg ataagctttc
    51541 tttatttcat cttctgatgc tccttttgag agccctaaga cttcataata atcacgtttt
    51601 gttgccatag gctttcctcc tattggttct catcattttc acgtcgcttg ttatcatatc
    51661 atattttgtt tgaaacaaaa accgatactt caaactattt ttcaaaagaa aaagccaaag
    51721 tagcaacttt ggccttctct cgaaattcag tgaactgatt atttatcgtc gccatcgact
    51781 tcttcaaaat cagcatcaac gacatcatct gctccgcctt gagctgcttc tggattttct
    51841 tgagcttgtt gttgtgcagc ttgttcgtat agtttaacag tcaagttttg aacgatttcg
    51901 ttcaatgcat cgcgtttagt tttcatttcc tcgatgttgt ttgcttcaac agcggctttt
    51961 aattcgtcac gagctgtttc tgcttttttc acttcttctt cgtccacttt gccttctaat
    52021 tctttcaatg ttttgtccac agagaatagc aatgcgtcta catcattacg taggtcaact
    52081 tcctctttac gagctttatc tgcttcagcg tttgcttctg catctttcac catacgttcg
    52141 atttcttcgt ctgttagacc tgaagaagat ttgatcgtga ttttttgttc tttttgtgta
    52201 cctagatctt tcgcagatac atttacaatc ccgtttttat caatatcgaa tgttacttcg
    52261 atttgtggaa taccacgagg agctgctgga atatctgtca attggaatct tcctagagtt
    52321 ttgttgtcag cagccattgg gcgttcacct tgtaatacat ggatgtctac tgctggttga
    52381 ttatctgccg cagttgagaa tacttgtgat ttacttgttg gaatcgttgt attgcggtca
    52441 atcaatttag tgaacactcc acccattgtt tcgataccta atgacaatgg tgtaacgtca
    52501 agtaaaacaa catctttcac atcaccagtg atcacgccac cttggatagc agcacccata
    52561 gcaactactt catcagggtt aactgatttg tttggttctt ttcctgtttc tttacgtact
    52621 gcttctacaa ctgcagggat acgagtagat ccaccgacta aaataacttc gtcgatttct
    52681 gattgtgaca gacctgcatc ttttaacgct tgacgtactg gtacttttgt acgttctact
    52741 aagtcagctg ttaattcatc aaattttgca cgtgtcaatg tcatttccaa atgcaatggt
    52801 ccagattctc ctgctgtgat aaatggcaag ctgatttgtg tgcttgatac gccagataaa
    52861 tcttttttcg ctttttcagc agcgtctttc aagcgttgta aagccatttt gtcttgagac
    52921 aagtcaatgc tgttttcttt tttgaattct gctaccatgt agtcgatgat tttgttatca
    52981 aagtcgtccc cacctagatg gttgtcgcca gctgttgaca atacatcaaa gacaccgtct
    53041 cctagttcaa ggatagatac gtcaaaagta ccgccaccaa ggtcgaatac taagattttt
    53101 tcgtcgcgat ctgttttatc taaaccatag gcaagagcag ctgcagttgg ttcattgacg
    53161 atacgttcta cttctagacc agcgatctta ccagcatctt ttgttgcttg acgttgtgca
    53221 tcattaaagt aagctggcac agtgataact gctttttcga ctttttcacc taaatagtct
    53281 tctgcgaatc ctttgatata ttgaaggatc attgctgaga tttcttgtgg tgtatatgat
    53341 ttaccttcta catctacttt gtaacctgct tcaccgatat ggcgtttgat tgatgaaatc
    53401 gtatttgggt ttgtcacagc ttgacgtttt gcaacttccc ctacttggat ttctccattt
    53461 ttgaatgata caacagaagg tgttgtacgg tttccttctg ggttagcgat gatttttgct
    53521 tcgtttcctt ccaatactgc tactgcagag ttagtcgtac ctaagtcaat accgataatt
    53581 ttactcataa ttaacgtctc cttctttaat tgaaagtgta agttttatat ccgcacaatt
    53641 attgtgcaac aatgaccata gttggtctta acacgcgatc atgtaatttg tagccttctt
    53701 gtagtacttc tacaatcgta tcggctggtg tatcttcagt cgctggaaca gtttgtactg
    53761 cttgatgaag attaggatca aaggcttctc ctttagcagg gattttttca attccttctt
    53821 cttccagtgc gttacgcaaa ctttccaaga ccatttccac gccttttttc aaacctgccc
    53881 cttgatcatc tgacacttct gtggctaatg cacgttccag gttgtcaatt gatggaagta
    53941 attttttggc taaatcttgt gaacggtatt tttgtaattg ctcacgctca ttttttcctc
    54001 gatttgccat atttgcgatt tcagcacgag cccgcaagta tttatcttcc atttcttcta
    54061 actcagcttt taatttcgct gtttcgaatt cttccgcttc agcttctgaa atacccgaat
    54121 cttctgcagc ttcgacatcg atttccggtt ctggctgagc atctgtcatt tctttatcta
    54181 gttcttcttg attttctttc acgatacagt cttcctttca ttattcagtc agctgtttga
    54241 ttgctcttac taagaaagag agaacgatca gctgcaaata gcttcgttat tcacattacg
    54301 agtttgacaa gtcaagcgag cggtagtaat cagcaagttt agctgctagt tctcttcgat
    54361 aaacatcgac tagaccgaac atccgagaat aaggcatact agtaggccct aacaaggcta
    54421 ttgtacctct tccatgacca tctatctcgt aacttgcttg aatcatactc atattctgca
    54481 ataaatcatt gcctaattca gcaccaatct tgatatgaat attgctatcc atcggagtga
    54541 tcatttgtgt aagtccgtct gagtctttca taaaagaaaa catcgatttg aactgatcaa
    54601 gatcttgacg gggctcataa tccaagagat tcatttgtcc actgacataa accttttctt
    54661 caaaaacatg accaagaacg gtgtcaaata actgagacat cccttctgtc gtctggaaat
    54721 atttatgaag aatcatcgga atctccgtac gaagtttatg gtacaccgtc atcagaggat
    54781 ctccgactag acggtcgttt acaatctgta ccattttttc tagttcctga cttcccatat
    54841 attcgggaag agtgaacact tggctttcta cattccccat atctgttacg acgatagcaa
    54901 taatctgtcg attattcaag ggtacgatac gaaagccagt cagtcggcgg tcttttattt
    54961 caggtcccaa tgaaaaagtc gtatagcttg tcagctcaga taatatctca gcagattgct
    55021 taataatctc attgatttca tgaaattctc tgcctagaga ctgacgaatc agcagcaaat
    55081 catcttctgc tacgcgagca ggttttaaca aatgatccac gtaataacga tagcctaaag
    55141 cagatggaat ccgcccagaa gaagagtgag tcttcaacag caatccttca tcttctaatg
    55201 ctttcatatc attgcgaatc gttgctgagc tggccttgac tccctcagac attaatgttt
    55261 tggaaccaac gggcacgcca ctactcgtat agtgttggat gatcagacgt aaaatgtcgt
    55321 tttgtcgctt tgttaacatc ttcctcacct ctcattagca ctcatatatt ctaagtgcta
    55381 attcacttat aaatatagca agaagccctt ctcctgtcaa gagaaagact ccatttttta
    55441 gcactctaaa cacaagagtg ctaattctaa ataattgatg taaatccagt tatatcaata
    55501 cttcaatacc tcattgtcaa actttctaaa ctttttcttt tcaagtttcg ataaaaaaca
    55561 aatattcttc aaactatttt tcaaataaaa agcgctcaaa cacattgttc cccaaaaaag
    55621 tccctcttgg tgtcaaataa acacggtctt cttcattaac aagtaaccct tcttgctcta
    55681 attcccttag cacatcaccg tacaccgatt caattgtttt ttgatagcga tcatagaaat
    55741 gttttttagt aatccctgtt ttttttctta gtcctaagaa catttcttct tctatttgat
    55801 tttttcgtgt caatttttct tcttccaata ccgggcgttc atttgctcgt aatgggcgta
    55861 aatagtgttg gattggtcct ttgttgcggt aacgaatatc tccaagataa ccgctagcac
    55921 cagctccaaa cccaaaatag tggtcattgt tccagtacat gaggttatgt tgagattcat
    55981 gtccggagag cccaaagtta cttatttcat attggtgacg tccatgcttt tccattgctt
    56041 caatcgtctc ttcaaacatt ctggtttccg tttcctgatc aggcagttcg agtcttcctt
    56101 gtcgtaccca gttcatgaac attgttttgt tttcgaggat caatgagtac aaagaatagt
    56161 gaggcagatc caaagccaaa gctttttcca aagtatcccg atatccttct agtgtttgtc
    56221 ccggtaatgc ataaatcaaa tcgatgctta cattggtaaa attttctgct tctaaaaatt
    56281 gcattgtttg atacacatct tctgcagtat gtttacgtcc aatttttttc aataagcgat
    56341 tgtcaaatgt ctgcactccc atggataaac gattgacgcc atagtttttc atcacttgaa
    56401 gtttttcacg ggtcaaatca ccaggattcg cttctacagt aaactcatta ccattttgaa
    56461 aaggcaggat ctctctagca cctgataata gtcgatcaag ttgtttagca gataaagaag
    56521 ttggtgttcc gcctcctaca taaagtgttt ccaaattcgc tgtaggatga ctttccagca
    56581 ttaatctcat ttcattcagc agcatttcca catactcatc tactggctgc ccttctaaaa
    56641 aaactttatt aaagtcacag taatagcaga tatgctcgca aaatgggata tgaatgtacg
    56701 cagaaacctt gttttctgcg ggatttattg tttctatcat tgaaattctt tctccttacg
    56761 tcgaaaagtc gtgcctgttt tcgaccttct taaaatcgcg ttcaagcacc aactcattga
    56821 aataagccgc atatttagaa tcaaggaaac ctagctctaa aaatgcttgc ttattgtcat
    56881 cggagctaca cggtgctttt acagcctctc gataaacggt aatcgcaaag ctgaccttcg
    56941 gcattaagtc aaaatctgca aaaatatgaa aagctatttt cacagattcc tccttaagtg
    57001 ctcagaccac aacgctttgc ttacaacctc tcatatacgc atactaaacg tatttggcga
    57061 acaaatccac taaaaaatcc aagaatcgac ttcttggatg atttttttac actcatgatt
    57121 cttcctaaac aattttgtcc ttattttatt cataaaaaa
//
LOCUS       DACECY010000010        55908 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.010, whole genome shotgun sequence.
ACCESSION   DACECY010000010 DACECY010000000
VERSION     DACECY010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.010"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            212..1426
                     /locus_tag="IWO38_000635"
     CDS             212..1426
                     /locus_tag="IWO38_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ0312750.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            1457..2884
                     /locus_tag="IWO38_000636"
     CDS             1457..2884
                     /locus_tag="IWO38_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0312751.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            2907..3833
                     /gene="glsA"
                     /locus_tag="IWO38_000637"
     CDS             2907..3833
                     /gene="glsA"
                     /locus_tag="IWO38_000637"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ0312752.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            4041..5501
                     /locus_tag="IWO38_000638"
     CDS             4041..5501
                     /locus_tag="IWO38_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312753.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            5722..7485
                     /locus_tag="IWO38_000639"
     CDS             5722..7485
                     /locus_tag="IWO38_000639"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ0312754.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            7569..8027
                     /locus_tag="IWO38_000640"
     CDS             7569..8027
                     /locus_tag="IWO38_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ0312755.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            8136..8819
                     /locus_tag="IWO38_000641"
     CDS             8136..8819
                     /locus_tag="IWO38_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ0312756.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            8966..9580
                     /locus_tag="IWO38_000642"
     CDS             8966..9580
                     /locus_tag="IWO38_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ0312757.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            9596..9802
                     /locus_tag="IWO38_000643"
     CDS             9596..9802
                     /locus_tag="IWO38_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312758.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            10341..10898
                     /locus_tag="IWO38_000644"
                     /pseudo
     CDS             10341..10898
                     /locus_tag="IWO38_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            10956..12995
                     /locus_tag="IWO38_000645"
     CDS             10956..12995
                     /locus_tag="IWO38_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ0312759.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVIGETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(13104..14255)
                     /locus_tag="IWO38_000646"
     CDS             complement(13104..14255)
                     /locus_tag="IWO38_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0312760.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(14409..14939)
                     /locus_tag="IWO38_000647"
     CDS             complement(14409..14939)
                     /locus_tag="IWO38_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312761.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(15038..15388)
                     /locus_tag="IWO38_000648"
     CDS             complement(15038..15388)
                     /locus_tag="IWO38_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ0312762.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(15545..17074)
                     /gene="ahpF"
                     /locus_tag="IWO38_000649"
     CDS             complement(15545..17074)
                     /gene="ahpF"
                     /locus_tag="IWO38_000649"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ0312763.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(17084..17647)
                     /gene="ahpC"
                     /locus_tag="IWO38_000650"
     CDS             complement(17084..17647)
                     /gene="ahpC"
                     /locus_tag="IWO38_000650"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ0312764.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(17791..18453)
                     /locus_tag="IWO38_000651"
     CDS             complement(17791..18453)
                     /locus_tag="IWO38_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0312765.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            18637..20283
                     /locus_tag="IWO38_000652"
     CDS             18637..20283
                     /locus_tag="IWO38_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ0312766.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            20274..21356
                     /gene="uxuA"
                     /locus_tag="IWO38_000653"
     CDS             20274..21356
                     /gene="uxuA"
                     /locus_tag="IWO38_000653"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0312767.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(21464..23017)
                     /locus_tag="IWO38_000654"
                     /pseudo
     CDS             complement(21464..23017)
                     /locus_tag="IWO38_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            23165..24306
                     /locus_tag="IWO38_000655"
                     /pseudo
     CDS             23165..24306
                     /locus_tag="IWO38_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(24432..25511)
                     /locus_tag="IWO38_000656"
     CDS             complement(24432..25511)
                     /locus_tag="IWO38_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0312768.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(25620..26060)
                     /locus_tag="IWO38_000657"
     CDS             complement(25620..26060)
                     /locus_tag="IWO38_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0312769.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(26117..26527)
                     /locus_tag="IWO38_000658"
     CDS             complement(26117..26527)
                     /locus_tag="IWO38_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0312770.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(26708..27142)
                     /locus_tag="IWO38_000659"
     CDS             complement(26708..27142)
                     /locus_tag="IWO38_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0312771.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(27242..28090)
                     /locus_tag="IWO38_000660"
     CDS             complement(27242..28090)
                     /locus_tag="IWO38_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0312772.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            28479..30245
                     /locus_tag="IWO38_000661"
     CDS             28479..30245
                     /locus_tag="IWO38_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ0312773.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            30347..31606
                     /locus_tag="IWO38_000662"
     CDS             30347..31606
                     /locus_tag="IWO38_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0312774.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            31753..33054
                     /locus_tag="IWO38_000663"
     CDS             31753..33054
                     /locus_tag="IWO38_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0312775.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            33057..33908
                     /locus_tag="IWO38_000664"
     CDS             33057..33908
                     /locus_tag="IWO38_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0312776.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            33921..34736
                     /locus_tag="IWO38_000665"
     CDS             33921..34736
                     /locus_tag="IWO38_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ0312777.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            34887..36053
                     /locus_tag="IWO38_000666"
     CDS             34887..36053
                     /locus_tag="IWO38_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0312778.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            36283..36930
                     /locus_tag="IWO38_000667"
     CDS             36283..36930
                     /locus_tag="IWO38_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ0312779.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            37014..37958
                     /locus_tag="IWO38_000668"
     CDS             37014..37958
                     /locus_tag="IWO38_000668"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ0312780.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            38093..39544
                     /locus_tag="IWO38_000669"
     CDS             38093..39544
                     /locus_tag="IWO38_000669"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ0312781.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            39565..40401
                     /locus_tag="IWO38_000670"
     CDS             39565..40401
                     /locus_tag="IWO38_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ0312782.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            40401..41060
                     /locus_tag="IWO38_000671"
     CDS             40401..41060
                     /locus_tag="IWO38_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0312783.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            41186..41875
                     /locus_tag="IWO38_000672"
     CDS             41186..41875
                     /locus_tag="IWO38_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312784.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            41881..42837
                     /locus_tag="IWO38_000673"
     CDS             41881..42837
                     /locus_tag="IWO38_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ0312785.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            42881..43393
                     /locus_tag="IWO38_000674"
     CDS             42881..43393
                     /locus_tag="IWO38_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0312786.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(43649..44197)
                     /locus_tag="IWO38_000675"
     CDS             complement(43649..44197)
                     /locus_tag="IWO38_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0312787.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(44286..45467)
                     /locus_tag="IWO38_000676"
     CDS             complement(44286..45467)
                     /locus_tag="IWO38_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ0312788.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(45703..47211)
                     /locus_tag="IWO38_000677"
     CDS             complement(45703..47211)
                     /locus_tag="IWO38_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ0312789.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            47370..47561
                     /locus_tag="IWO38_000678"
     CDS             47370..47561
                     /locus_tag="IWO38_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312790.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            47764..48681
                     /locus_tag="IWO38_000679"
     CDS             47764..48681
                     /locus_tag="IWO38_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ0312791.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            48674..49747
                     /locus_tag="IWO38_000680"
     CDS             48674..49747
                     /locus_tag="IWO38_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312792.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            49895..50413
                     /locus_tag="IWO38_000681"
     CDS             49895..50413
                     /locus_tag="IWO38_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312793.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            50394..50786
                     /locus_tag="IWO38_000682"
     CDS             50394..50786
                     /locus_tag="IWO38_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ0312794.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            50862..51797
                     /locus_tag="IWO38_000683"
     CDS             50862..51797
                     /locus_tag="IWO38_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ0312795.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            51930..52709
                     /locus_tag="IWO38_000684"
     CDS             51930..52709
                     /locus_tag="IWO38_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ0312796.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            52702..53019
                     /locus_tag="IWO38_000685"
     CDS             52702..53019
                     /locus_tag="IWO38_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0312797.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            53263..54873
                     /locus_tag="IWO38_000686"
     CDS             53263..54873
                     /locus_tag="IWO38_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0312798.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            55030..55878
                     /locus_tag="IWO38_000687"
     CDS             55030..55878
                     /locus_tag="IWO38_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0312799.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        18535 a         9920 c        10900 g        16553 t
ORIGIN      
        1 ataaaaaaag acgaaaataa acggttttct ataaaaagaa gaccgtttat tttttgtctt
       61 tatttgtgtg aaaaacgaaa aagagcacaa cacatcaagc ttttattaat tgtctgtctt
      121 ttataaatgc aagtattaat tcaaaaaaac agaagaaaaa aacaactaca ccttctttta
      181 tactcgtaat cgtagaaagg aggcgaaaaa aatgaataca ttacctatca atacaggaga
      241 tacagcattc atgattttat gtacagcaat ggtttgcctg atgactcctg gtctagcttt
      301 tttttacggc ggattagcac ggaagaataa tatactaacg atcatgtctc aatcttttct
      361 ctccgttggg ataacaacga ttatgtgggt atttggtgga ttcggattgg cctttggacg
      421 ggatatcggc ggtgttattg gaaacccggc agatttcttt ttgatgagac atgttacaag
      481 cttccccaac atgtttcatg gtgcaacgat tccattttta atgttctttc tttaccaact
      541 gatgtttatc gtgattaccg ttcctttgat gacaggagct tttgcaggga gattgaattt
      601 aaaaggctat attatacttg ttattttttg gaatctgctg atttatttcc ctgtttgcca
      661 ctggatatgg ggtggcggat ttttggatca gatgggattt agagatttcg ctggtggagc
      721 agtcatccac acgacagctg gattcggctc tttagcatgt atcctcacac ttggtcaaag
      781 aaagattcct ttaggagaaa gaagtcagca tagcagtctt atggcagctg ctatcggtac
      841 aggactgctt tggtttggct ggttcggttt taattccggt ggtgcacttc gagcagatga
      901 acaagcagtc aatgcatttg gttcaacatt catcgctctt gcatttgcga tgattacttg
      961 gctgatcatt gcaaaagtaa aaggaaatgg ctttgatttt gtcgacgtac taacaggatc
     1021 cgttgctgga ttggcagcca ttacgccttg tgccggttat gtagaagcaa aaagtgccat
     1081 gctgattggg atcattgcgg gtatcgtctg tcatgctgct gttgattttc gtaaaaagaa
     1141 acaatgggat gatgcacttg atgtgtgggg tgttcatggc atgggcggat tcacaggtac
     1201 gattctgata gggatctttg catctaacag ccatttacta acgaatccaa gcagctggta
     1261 cttcttaggg attcaagtga ttggtgtcat cattactgcc gtgtatgcct atgtgctgac
     1321 aacagtgatt ttaaaatcgg cgatgcactt tacaacgatc actactacaa aagaagaaca
     1381 agaagaagga ttggactttt tcttctttgg caacaaactc aattgaaaca aattcaaata
     1441 gaaaagagga agattcatgc aacaaagcaa aacgaaaatt cctgcaaaaa aattaacgtt
     1501 ctttggcttt ttggctatga ccatctcaat ggtcgtttca ttatatgaat atcccacatt
     1561 cgcgacctca ggcttcagct tagtattttt cttgttatta ggcggtttgc tatggtttat
     1621 ccctgtggca ttgtgcgcag cagagatggc aacggtgaaa ggatgggaaa aaggcggtgt
     1681 ctatacttgg gtcagtcgca cattgggaaa acgattcggt tttgccgcaa tctttttcca
     1741 atggtttgag ataacagttg gctacttgac catgctgtat ttcttaactg gtgcattatc
     1801 ctatgcaacg ggtattggtg ctatacaaaa caataaatat ctgaaattgg caatcctatt
     1861 gattattttc tgggctattt tactctcaca gttaaaagga accaaataca cttcattgat
     1921 tgctcggatc ggttttgtag ctggtatctt gttgccagca ttagtcttgt ttggcttagg
     1981 gatccattat gtcgcttcag gcgctccact acaaacgata ctgtctatga aaacattgat
     2041 tcctgatttt tcgaaattgc ctacgctggt tgtttttgtt tcctttattt tagcatatat
     2101 gggcgtagaa acatctgcca gtcatgcaaa tgaaatggaa aaccctaaaa aggattatcc
     2161 attagcaatg tttgtattgg ttattctggc aatcatttta gatacttttg gcggattgac
     2221 cgtcgctact acagtacctc aacatggttt gtctcttaat acaggggtga ttcaatcgtt
     2281 agaattccta ttaagacaca ttgatccaaa tcttgcatgg attgcgaaaa ttcttgcgat
     2341 tcttgtttgt ttaggtgtca tcggagaaat tgcttcttgg gtaacctctc cttcaaaagc
     2401 acttcacgta gcagcagaag aaggattact tcctgaatat tttgcaaaag aaaacacaca
     2461 cggtgttcct gctcatttga tgatcgcaaa tgggatcgtt gcaacaatct gggcagccgt
     2521 ctttacatta agcggcgggg ggaataatct ttcatttctt gcagcgatgt cattgactgt
     2581 tgtaatctac ctgatgttgt actttatgtt tttcgcgggc tatttgaagc tggtttttaa
     2641 agatgacagt cttgaaagag cctatcaggt tccaggcgga aaagttgtaa aagtaatcgt
     2701 tgcttgttta ggtcttttga cttcactttt tgctttttgt atctcatttg ttccacctgc
     2761 tagtatcgca gctggacagc atgggattta tgaaacgatt ctgattgttt cttatcttat
     2821 cgtcttagtt attccattta cgatctacgc atgccgtcat aaattccagc atgcagaaaa
     2881 ataaaaaatg aaagctggtt attcaaatgg aagtaaaaga attagaacaa ttgattggaa
     2941 aaaatttcca ctattatcaa acaggacaag tggcggatta cattccagca ttagctgaag
     3001 tggatcccaa acaattaggt atggcgattt atgatttgga tacaaaacaa ctgattgagg
     3061 gaggagacgc cgatgtgcgc tttgccatcg aaagcatgtc aaaagttccc gtattgttat
     3121 tagcaatcaa agacaacgga atagaaaaag tctttcaaac aatcaacacg gaaccaaccg
     3181 gttttgcctt caattctatc atgaatatgg aaatcaatca tcgcaaacat ccaatgaatc
     3241 cttttgttaa tgcaggagcg attgcaacaa cctcgttgat tgccggtaaa gatgcagatg
     3301 aaaaatttga gcgaatcttg tcatttatga aagagatctg tgatgatcct aatatcacat
     3361 tgaatgaaga aatctatcat tctgaatcaa gaacaggtga tatcaatcgc tctcttgctt
     3421 actatatgaa aggtaatcaa atgatcgaag gagacgtacc ggagatactc gatgcttatt
     3481 tcagacaatg ctccctcaat gtcaccgcta caggcatagc aaaattagct gctgtgctgg
     3541 caaataaagg aattgctcct tggaatggca aacaaatcat tactgaagaa agtgctacaa
     3601 tcgtcaaatc aattatgaca actgctggtt tgtatgatga atcaggagag ttttccgtcc
     3661 atgttggtgt acctgcaaaa agcggtgtag gcggcgggtt gatggcagct gtgccaaatc
     3721 gttacggcat aggtgtcttc agcccggcac tagatccatt tggtaacagc gcagctggta
     3781 tccaattgtt gaaagatgta gtcaaagaac tagacgctga catctttgaa taaccattct
     3841 tgaaagaggc tggggtaaaa gtccaaacgg ctcaaagcta cctacaatga ggctgaaatt
     3901 gtgagcttgt ttgacttttg tcccagtttc tttttttatt ggtttttgaa agggcttttc
     3961 taaataaaaa ggaaacaaaa atacgattta cagatatgat taaataaaat cataagaaaa
     4021 actggaggcg tgtatcagga atgctggaaa attacatcga gcgaaatata tttagaaaag
     4081 tgtatctgtg tgaacaatta tttgaattcc aagaaataga catcgaacaa acagctattt
     4141 ctctaagggt gactacacca acgatccttc acgatctaga aagtctagcc gagtgtctag
     4201 aatattgtat aaaagaacaa gtaagagaga agcataagta taaactcgta ttcaaacatg
     4261 gtattgctct ttctgagctc acacagtttc tgtacggtca atcttatttt ttaaagtttt
     4321 taagttatta ttttaatggt atattcacga gtacagaact ggctgatctt gaatttattt
     4381 ctttgagcaa agtatataca atcaaaaaaa tcgtattgga tttttttaaa gaaaatgatt
     4441 atttgaaaaa taaagaaatc attatcccag aatttgactc gcggaacatc ttgctagctt
     4501 tagtacgtta tgtcaattgg gaaggctatg aaaataaaaa tccgtatgta gaagaagctt
     4561 gtaacgagtt gattgattat gtagagactc atttttttaa aagaagatat tcggaagatg
     4621 aaaagtcctt tatcatacga ggaatagaga tcgcaatcgg aagaaaggca tttccccttg
     4681 gactgaaaga agaagataag aaaagcgcag aaacgaagcc gttatttcaa ttagtctgtc
     4741 aaggattgga agagcaaaaa ggaattctag acttacaaga agaagatgtc tattatattt
     4801 tttctttgtt caacagtaga aattatacca atgagaacat ggagctgtta agaaaagatt
     4861 tcgaagttgt ttataaaaat tttgttctca atgacctatc actgaatgaa ttgattttac
     4921 agattattag tatcgctgaa atcgacgatg cagaaaattt tattttcaag caatcctttt
     4981 tacaatttgt ccgtacgttg tgggcagatg gacaagtatt tttaccggaa aggatctatc
     5041 ttctatcaga aaaagaacaa cttctgtacg gaaaaattgt agaaatattg gaagagtgga
     5101 aaaagaaaaa tcaaatccaa ctaagatgga atgaaaattt cattcgaaaa ttcgtcaaaa
     5161 gtctttcttt gatcaacagt gaacaagcgg ggagacagga aatagaaatt tttgttgttt
     5221 cagaaagttc agtcaaacaa tttttttatc gaacccaatt tcatttgtat gcggaagaag
     5281 gcatcgctga aataaatcct attttatttc gttcattaga agaattgcct gatgaatgtc
     5341 tatgtgcgaa aaaaagagtg gttttgtgcg atgctgctag ctatcaaaat ggattagaaa
     5401 ctgaaaagac aaagatctat ccaatctcat taaaaggtgc aaacatccat ttgtatcata
     5461 ttgctcagga acttcaccta ttaaaaaaac atatttcttg atgagacctt aattaggaga
     5521 ccttctacaa agctttttaa ttgtctgtag aaggttttta tttgagtcct gattcgtgcg
     5581 aagatctact tatataacaa gctttctctg aaattttttt aaaatgctat tgacttagag
     5641 tcaactctaa gtagtagaat agataaagtg taacaaatct attttcatta aaagaagata
     5701 gtacggagga gagaatgtat tatgagtaca gtattacatg tattggatgc aatcaatagc
     5761 cccgcagatg tcaaaaaatt atctgttgag gaaatgaaac agttggcaga agagatgcgc
     5821 caaaaaatct tgcaaaagga cagcgatgta ggtgggcacg ttggtccgaa tctaggtgta
     5881 gtagaattaa cgattgcgtt tcattatgta tttgattcac caaaggataa aatcgtttgg
     5941 gatgtttccc atcagtctta tggtcataag cttttaactg gtcgaaaaaa tggttttgaa
     6001 aaaggacatt accgtgaagt gagcggatat tccaaccaac atgaaagtaa acatgactat
     6061 tttacagtgg ggcacacatc tacatctatc agcttagcaa caggtttagc taaagcgaga
     6121 gatctgaaga aagaatcagg aaatgtggta gcatttatcg gtgacggctc cctaagtggt
     6181 ggcttagctt ttgaaggatt gaacaatggt ggtgttttac aatccaatct aatcgtatta
     6241 gtaaacgata atgagatgtc tattgatgag aatcatggca gtttatataa aaacttagct
     6301 gagcttcgtc aaaccaacgg taaaagcagc aataacctat ttaaagcaat gggatttgat
     6361 tatcgttacg tggataatgg aaacgatctg gaaacggtga tccgtttatt tgaagaagta
     6421 aaagatattg atcatccgat tgtacttcac gtccatacgg aaaaaggtcg cggttatcag
     6481 ccggcaatcg ataataaaat gaaatatcat tggcatgtac catttgatct gtccaccggg
     6541 gaggcaaagc aagtaacttc tggagaaaca tttaatatga tcatcatgga ttatttggat
     6601 aagaaaatgc aaacaagtac agctcctatt gccgcagtca atgcagcgat accaggcgcc
     6661 tttggactga aaacattcca agaaaaacat ccagatcatt attttgacgt ggggatcgct
     6721 gaacagcact ctatcacttt cgcttctgct ttggcttctc agggagtacg tccagtcgtc
     6781 ttccaatcaa gtactttctt gcaacgggca tatgatcaat tatcacatga tttagcgatc
     6841 aacgagaatc cagtgattat tttagtcggt ggcggaacga tctcagctag tgaccgaaca
     6901 caccaaggaa catttgacat tccttactta tcaagtatcc cgaacatcaa atatcttgct
     6961 ccagcaacaa aagaggatat gatctctatg ctagattggg cgttcgaaca aacagcgcag
     7021 cctgtagcaa tccgaattcc tacacatggt gtagaacatg gtacagctat cgcaaatgat
     7081 tatagtattc cagcctatca aacagtaaat aatggaggaa aagtagcaat tttagcttta
     7141 ggcggttttc ttgaattagg aaaagaaaca gtaaaagctt ttagaaaaaa aggaatcgaa
     7201 gcaacgttga tcaatccagg aacgatcaca gaaaatgacg tagaaacatt agataaatta
     7261 aaagaaacac acgaattagt catcacatta gaagatggta gtttgtcagg aggatttggc
     7321 gaaaatatca gtcgttatta tggtgattct tcaatgaacg tattaaattt tggtgcagag
     7381 aaagaattca cggatagcgt tcctgtaggg cagctttatg aacgttatca tttaacacca
     7441 aaacaaatcg ttgaagatag tctgcgtgtc ttgaatattc aataattcat tatgctcacc
     7501 atccagataa ttgtttatac tagtatctgg atggtttttt cattataaaa agcaaagggg
     7561 tggatgttat gtatcagcaa gtagaagaat atgtcaaaaa gttccaagaa aaatatccta
     7621 gttatcaatc agaaaaatta ggtttctttt catttgggga tacagaaaaa atggcagacg
     7681 agttgagcga actagttgta aaaaagataa agactggtac atcaagtggt tacgatctgt
     7741 ataaacaaga gaatgagcct ttaccaaaag ttggtcaatt agacgtggtc ctgaatggaa
     7801 aaaatgaacc agtttgtatc atcaaaaata taaatgtgac catcttgcct tttagtgaag
     7861 tcacgaaaga gcatgcttgg aaagaaggag aaggggatcg gacattagct tattggcgga
     7921 aagtccatgc tgagtttttt aaacatgctt ataaagaagc gttgaacata gaatttacag
     7981 aagagaagct ggtcgtatac gaagaatttg aagtaatttt cttttagtaa gaaaggtctg
     8041 ctgaaagaaa tagctcatta ttgtctcctg aatagaaaag ggttacattt ttatatgaag
     8101 ctgtattttt atataacatt actggaggag atattgtgga tacactggaa caaatcaaag
     8161 gcggagtcgt cgtttcttgt caagctttgg aaaacgagcc gctacacagt tcttttatta
     8221 tggggagaat ggctttggct gcaaaagaag gcggagcagt tggtattcga gcaaatacga
     8281 cagaagatat caatgaaatc aagcgaatcg ttgatttacc ggtaataggg attatcaaaa
     8341 gagattacga tgggtcagaa gtgtttatta ctgcgacaga aaaagaagtg gaagaacttt
     8401 tggagacgca agcagaaatc attgcactgg atgcaacttc tcgtaaacgt ccgacagaaa
     8461 aaacaactgc tgaacttgtc caaatgattc acgaacacgg tcggttagct atggcagata
     8521 tttcgacttt tgaggaagcg cttcaagcgc aagaagatgg ttttgatctt ttgtctacga
     8581 ctttagcagg ctatacagaa tattcacgaa agacgcagac acctgatttt gatttattga
     8641 atcaaatcat agaacaagtc gatatcccag taattatgga aggacatact gactctcctg
     8701 aacaagtaga gaaagcatat gaattaggtg ctttttcagt cgtagttgga tctattatca
     8761 cccgtccgca attgatcact aaaagatatg tcgaagccgc aagtaaagca aataactaaa
     8821 aaacacgcac ggcacagatt cttgacaaaa aagaaaaatt gatagatagt ttaaagcaga
     8881 ttctaagaag atgaaaaaag cattactttt cattttgtct tcaagatctg ctttttaacg
     8941 aattttatag aaagaaggcg aaaccatgtt tgaacggagt ccttcagaat tgacagaacg
     9001 tgaaaattac aagttgctga ttggttcagt catccctaga ccagtagcag tagtaacgac
     9061 taaatcagca aaagatatag tgaatatcgc acccttcagt tacttcaata tcgtcagttc
     9121 gaatcgaccc atcttatcct tggcgatcca gagaaaacaa gatgcagtaa aagacacagc
     9181 aaagaatatt cttgagacaa gagaagctgt tatccatatc cttgatgagg acaacgtaga
     9241 agaagtaaat aaaacagcag ccaatctgcc taatgaggaa agcgaactgt acctgactga
     9301 cttgacttta actgattcag taatcatttc agtaccagca ttgacagaag caaaagttcg
     9361 ttttgaaaca tccttatatg aacatgtcga gatacggaat aaaaataaag taacagctga
     9421 tttatttttg ctagaagtac agaaatatca tttaaatgaa acagtctatg atgataagac
     9481 aggaaggatc gatccaagaa aattgaatgc tgtaagccgt ttggcaggaa atgattatgc
     9541 agcaatagga gaaattttca cgattgcacg tcccgaataa aaaggagcac tagttatgtt
     9601 aaaaataagc tataaaccat cgacagattc aaaagaaatg aaaaaagaat atgaaacagt
     9661 gaatgatttt ctgcaagggc agtacttaga agttccgcca ttgcaagacc attttgtcgt
     9721 tactactgtt actttagatg gtaaggaaat agaaatgcca gatcaaacga ttagcggatt
     9781 gtttaattac tttaacaaat aaatatttat tccgaaaagt gaaaaaacat ataaaaaaac
     9841 acataaaact aaaaaattat caaagaatcc tggtgcaact gaaagaattt gcaacgggat
     9901 tctttcaact tatttgttat ttttttctaa aaaatattaa agattgttaa atttcactat
     9961 ttcatttcaa attccattgc ctaatcatta ggaaaagtct atcatgaact cgtaaataga
    10021 atatagtaat aaccttgtta ggtgaggctc ctatacaaac ataggctgtt gcccagaaac
    10081 gtcgaaagtc gccaatgggt aaaacagaaa ctgttggatt aaggcttttt ccaatagcta
    10141 agaatgtttt ctttacgttg tatagtgcta aaactcggac gatgagatga tgctaggtca
    10201 gaattcttgt tcgaaaaggt ttctgacctt ttctttatat ttttgtgact gacttatttg
    10261 atgagacaaa ctcgctcctc acctgacaag tggattgctg tgtgaggagg agatgcggtg
    10321 aaaaaagatc gattggtgga ggtcgctcgg attcacttct ggttgcacaa tacaaccaga
    10381 aaacacaaca agcaaaactg atctctatca tgagagattc atatgtcgat attccaggtt
    10441 acggaatgaa taagataaat gcagcttact cttatggggg agttgattta ttaaatcaaa
    10501 cattaaagga aaatttcaaa tttgaagctc cctattatgc aagtatcaca tttcaagatt
    10561 ttatcgattg cgttaatgaa ctgtttcctg gtggagtcaa gatcgatgca gaaaaatctc
    10621 tagatttaga tggcgtatat ataaacaaag gagagcaagt gatggatggt aatactttac
    10681 tccagtatgc tcgatttcgg gaagacaaag aaggagactt cgggaggatc agaaggcagc
    10741 agcaagtgat caaagctatc tctcagcagc tgaaagacgt aacttcaata ttcaagctac
    10801 ccaaagcggt tgggaaatta cttggtagta tccaaacgaa ccttccagaa agtgtgcttc
    10861 tggattgtgg tatggatttc ctcaaagaca tatgttagaa taaacaggta taaatagtga
    10921 aataaaggaa tgacaagcaa gagaaggagg aaaaaatgaa aagaagtgac aagcacggca
    10981 aaaatcgaac aggcgcttat attgccggcg cagtgatctt aatagcagct gcgggtggcg
    11041 gttattttta ctaccagcac tatcaagaaa cccaagcagt agaagctgga gaaaagacag
    11101 ttgagcaatt tgtccaagct ttgaacaagg gagattataa caaagctgca gaaatgacat
    11161 caaaaaaggc ggcaaataaa agtgcattat ctgaaaaaga gatcttagat aaataccaga
    11221 atatatacgg tgctgctgat gtcaaaggac ttcagatatc gaatctaaaa gtagataaaa
    11281 aagacgattc tacttacagt ttttcatata aagcaaagat gaatacctca ttaggtgaat
    11341 tgaaagatct ttcttataaa ggaacattag acagaaatga tggtcaaacc acgatcaact
    11401 ggcaacccaa cttggttttt ccagaaatgg aaggaaatga caaagtaagc ctgaccacac
    11461 aagaagcagc aagagggaat attatagatc gaaatggtga accattagca acaacaggca
    11521 aactaaaaca attaggagtc gttccaagta aacttggaga tggaggcgaa aaaacagcca
    11581 atatcaaagc gattgcttcc tccttcgact taacagaaga tgctatcaat caggcgattt
    11641 cacaaagctg ggtacaaccc gattactttg tcccattgaa aatcattgat ggagcaacgc
    11701 cagaacttcc agctggagct accattcaag aagtagacgg cagatattat cctttgggtg
    11761 aagcagctgc tcagctgatt ggttacgtgg gagatatcac agcagaagat attgataaga
    11821 atccagaatt aagcagtaat ggaaaaatcg gtcgctcagg tttggagatg gcttttgata
    11881 aggatcttcg tgggacaaca ggtgggaaat taagcattac agatgcagac ggtgtagaga
    11941 aaaaagttct gatcgaacat gaagttcaaa atggcaaaga tatcaaattg acaatcgatg
    12001 cgaaggcaca aaaaatagct tttgacagtc taggaggaaa agctgcatca actgttgcga
    12061 caacgccaaa aaccggtgat cttcttgcgc ttgctagctc tccaagctat gatccaaaca
    12121 aaatgacaaa cgggatctca caagaagatt acaaagctta tgaagaaaat cctgaacatc
    12181 cattcatcag ccgatttgcg acaggttatg ctcctggatc tacgtttaaa atgattacag
    12241 cagcaatcgg tctcgacaac ggcactatcg atccaaatga agtgttgacg atcaacgggc
    12301 ttaaatggca aaaagacagt tcttggggat cttttaaaac aacgcgtgtc agtagtgatg
    12361 tatcacaagt agacttaaaa actgctttga tctattccga taatatatat gcggcacaag
    12421 aaacgttgaa aatgggtgag aaaaaatttc gtacaggctt agataaattc atttttggtg
    12481 aagaccttga tttgccaatt agtatgaatc cagcacaaat ttctaatgaa gatagcttta
    12541 actcagatat cttgctagct gatactggat atggacaggg cgaacttcta attactccta
    12601 tccagcaagc agcaatgtat tctgttttta ccaacaatgg cacacttgtc tatcctaaat
    12661 tgattgcaga taaagagaca aaagataaga agaatgtcat cggcgaaaca gcagtacaaa
    12721 cgatcgtgcc agatctgaga gaagttgtgc aagatgtaaa tggtacagca cattctcttt
    12781 ctgctttagg gattccgttg gcagcgaaaa ctggtacagc ggaaatcaaa gaaaaacagg
    12841 atgtaaaggg gcaagagaac agtttcttgt ttgctttcaa tcttgataac caaggttata
    12901 tgatggtcag catgttggaa aataaagaag atgatgattc agcaaccaaa cgagcatcgg
    12961 aactattgca atacctcaac caaaattatc aataaaaaat tgctttttaa gtacagtaaa
    13021 aggactgtgg cgttatcgtc acagtccttt tttcaaataa acggttttca cgatgtctct
    13081 ttcacaactt ctttttgatt tactcacact tcataaactt tttttgtaaa atatttgaga
    13141 acaagtggag aaataagtgt acttaataaa atcacgataa ctaaaggtga atagtactgt
    13201 ggttcgatca attgactttg ctgtccaatt tgtaaaatga tcaaagccat ttctccacgc
    13261 gaaatcatac cagcaccgac catcaatgaa ctatgttgag aaaaaccggc aatctgagca
    13321 ccaaaataac ctccaagcaa tttggtcaaa atcgctacaa gcgtcaatat agaaataaag
    13381 agtagttgtt cactaaagtc agaaaaatca acttctaatc ctacactgac aaaaaatact
    13441 ggtataaata ctgcatatcc taaagcctct acattgtggt atacttcttc tttaaccttt
    13501 gtctgactga cagctatccc agcaaaaaaa gcaccaatga cagagctaag cccaacaaga
    13561 tccgctacgt aagacattcc taaacaaata actagcgata cgatgataat agaagaattt
    13621 gcgtacactt tttctgcaaa tgaaagtagg aaaggcgcaa tccatttgac taataagaaa
    13681 ataaaaacaa aataaattag ctgttcaatc aaaacaagcg gtaaagaagc attgctagaa
    13741 gcttcccccg ttagaaaaga taggctgaaa cttagaacta aaacgactaa aatgtcatct
    13801 accacagaag cacccaaaat tgttgatcct tcttttgtat tcactacgtt tagttctttt
    13861 aatacttcta ctgaaatact gacagaagtt gccgcaagaa taatcccaaa gaaaatcgct
    13921 tcatttcccg aaacttggaa tgctttgcct gtcaaccagc caaaaacaac cgggaagatg
    13981 atcccaagca aagcaacgaa cataccaggt cggaaatatt ttttcaaaag agataaatca
    14041 ctttccagac cagctaaaaa cattaataag atcacaccaa tctccgaaaa gtcatggact
    14101 agaatatcag gtttgaccca attcaatcca gcttggccta gcaaaacacc aacaagtaat
    14161 tgtccaatca ctgctgggat acctaatctc cgcgagatat gtgagccaat cgttgttgaa
    14221 atcaatatca ggcataaaat accaataaat tccattctca tcattccttt gtataaaata
    14281 tagcccgaac ttagcataac atacattatt gaaaaaatag gaaaatatac tcacaaaaaa
    14341 accaacaatc caaaagattt ttttggattg ttggttttta tacggtctat tttaataact
    14401 aaaaatgatt aacgtatagt catttttctt attctgatga aattgataat ctcctggatg
    14461 cccatgaaaa tcaaaataaa gccgaatatt tggaatagaa gcatcaaact tttgaatgga
    14521 ttgaacaata agacgaatcc gccaatcaac aacagaatac tactataaac actccaactt
    14581 ttagcttttg tattcatgct gaagaacaac tggaacaagc tgttgatgat aacagctaat
    14641 ccaagaagaa tcggcaggat agaaacgatt gcaggtgcaa aagcatagac gatcagagct
    14701 aagatcagga aaaaaagacc actgaacaga ccgatactcc aagaacctgt tttcctttta
    14761 aaacggtaag cctcaaaaag attcaacaac ccaagcaaaa caagataggc ggtgatcaga
    14821 taaccgataa aatgaaaaac agcagaaggg tttagcacaa tcgcaatccc agaaataata
    14881 taaacagcag ctcttaataa ggcgtatttt tgaatatttt gtttaatcgt ctcaaacata
    14941 taagaaaacc tcctttgact tattttaacg tgaactagtc attttaccaa attaaatgtt
    15001 acaaaatgag cgaaggagaa gaatatctct ggaaaaatca acttctgagg atcttttcca
    15061 tttttttgcc ttttgctagc tcatcgatca ttttatctaa ataacgaatt tctttcatca
    15121 agttatcctc gatgtcttct actcgtacac cacaaacaac acctgtaatc aaggaacgtg
    15181 aaggattggg gacaggggct tccgcaaaaa attgttcgaa agtcacttgt ttttgtagtt
    15241 gatattctag ttctgtttgg gaataacccg tcagccaaca aatgatttca tctacttcct
    15301 cttttatacg atcttttctc tccgcttttg ctacatacat aggataaacg cttgcaaaac
    15361 ttgtcgtaaa aatacgattt tgcgccataa tcagtcacct ttcttttttt caaattattt
    15421 actacttata gtatataaga aaaagagaag aaaaagcgat tattcaaaaa aacaaaaagg
    15481 attgtgacaa aattcttgtc accatccttt tttccgctct ctgacttttt acaatcttca
    15541 tagctcaagt gcgcatcaga taatcgaatg ctcctaaagc tgcagtagct cctgaaccca
    15601 ttgaaacaat gatttgtttg tatggactat ctgtacaatc accagctgcg aaaacgccgg
    15661 ggatattcgt tgcgccattt ttctcggtca ctatttcatt tcttggattc atttcgatcg
    15721 ttctatctag ccaatccgta ttagggagta agccgactag aatgaatact cctgaaacag
    15781 ctagtgtatg catctccatc gtacggcgat cgatatagga gagagattct acctgattcg
    15841 taccgctgat ttctttcgtc gctgcatgtt tgataacagt gacattcgca agagagacta
    15901 gtttatcctg taagatttga tctgctttta attctggtaa gaattctaga acatatacat
    15961 gttttgctaa cccagccaaa tcgattgctg cttcaacacc agaattacca ccaccgataa
    16021 cagcaatctc ttttccttta aataaagggc cgtcacagtg tgggcaataa gcaattcctt
    16081 tatttttaaa ctctttttcg cctggtacat tgatagaacg ccagtgcgca cctgtcgcga
    16141 tgatggcggt ttttgcttta agtgatatac cgttagctaa gccaatatta ataaaatgtt
    16201 gctctttatt taaggaaacg gcctgttgat tcatcataat gtcgataggg taactacgca
    16261 catgactttc tacttgctgc atcaattttg gaccttctgt gtaagaggta ccaatcatat
    16321 tttcaatccc tagtgtttca acgacttgtc cgccgaaaga atcagcgaca agtcctgtac
    16381 ggattccttt acgtgctgca tagatagctg cactactagc tgctggtcca ccgccaatca
    16441 ctaatacatc ataaggttct ttttcaaaca actcttcttc aatcaaaggg ccagtgattt
    16501 tttcaacaat ttgttcgatc gtcatgcgtc cgctactaaa ttcttctcca tttaaaaaca
    16561 cagttggcac tgccatgatt tttttatcat cgatttcttt ttggaacatc ccaccttcaa
    16621 tcatcgtatg agtaatattt ggatttaata cagctaatat attcaatgcc tgtacaacgt
    16681 cggggcaatt atgacaagtc aagctaacat aactgtcaaa atgcaatgtt tgtgttattt
    16741 ttttgatttt aagactcaaa gattcactga ttttcggtgc ccgtccacta atttgtaaca
    16801 tagccagtaa aaaagaggaa aattcatgac cgagggggat accagcaaaa acgattccac
    16861 tttctttgcc aactggattg atagaaaaac taggtgtgcg catcgaatct cttttttccc
    16921 aagagatttt aggagacatt tctgctattt ccttcgtaaa ttcagctagt ttttgactat
    16981 tttcactatt atccaaacta acagaaaaaa caataggaga ttcaatcagt gctaaatatt
    17041 gttcgagatt ttgtttagtt tctttgtcta acatatccac cacctaaatc tttccaacaa
    17101 gatcaaaact tggtttcaat gtttcgccac tttctttcca ttttgccgga caaacttcac
    17161 ctgggtgggt acgtacatat tgagcagcgc gaatcttatc aatcagtgca ctagcgtcac
    17221 gaccgattcc atctgcattg atttccatag cttgaacgat tccgtctgga tcaataataa
    17281 atgttcctcg ctgagctaat ccttgctctt catccaaaac atcaaaaata cgagaaatct
    17341 gatgagacgg atcaccgatc atcacgtatt cgattttacc gatagcatct gatgtatcat
    17401 gccatgcttt gtgagtaaag tgggtatcgg tagagcaaga aaagacttca acacctaaag
    17461 atttcaatgt actgtattgt tcttgtaagt cttctaattc agtaggacaa acaaaagtaa
    17521 aatcagctgg atagaagcaa agaatactcc agttccccat aagatctttt tcactgactt
    17581 ctaaaaattc tccttgatga taagcatgtg cattaaattc ttcgattttt ttcccaatca
    17641 atgacatagt aaaacctcca ataataatta tttatttata attattgtaa tttagttgga
    17701 gttgcaaagt caatggatat tttgttattt aataaagatt actcaaaaac gaaggttaat
    17761 tataaaaata gaagaggtcg caagaaaatg ctactgctgg aaatactcgg gataacgttg
    17821 agtaacagtt tcctgttctt tcagcatcat gtgtaagtgc aatcccgttt gaaatcgagc
    17881 ttcttctggt tttttatcaa ctaaagcctt gagtaaatca taatgttgtt ccatgatcgt
    17941 ttcccatgga agcccctgaa tggtcactcg taaccagcgg aaacgttcta aatgggtatt
    18001 atggtcatct aaccagttcc aaacttcttt tcttcctgca atttcgaaac aagcttcatg
    18061 aaataaatta tctgcacgga aaaaagcgcg ttcgtcattt attttgactg ctttttcttg
    18121 ttcttctaaa atcgtttcta gcactattcg tgtttgtgtg tccaatttgg aacaacattc
    18181 aatcatgatt tgctcttcca actgttctcg gacaaagcga gcattttctg cagaaacaag
    18241 attaattttg gaaacatacg taccactttg aggtatcgta tacacgagtt cttgctgacg
    18301 tagttgaatc aatgcttcgc gaatgggagt ccggccgata gcaagatgtt cctccagtgc
    18361 cttttcagat attttccttc caggttctaa gtcagcatag ataatttgtt tacgaatcgc
    18421 ttgataagct tgtgattgca agctgctttt catctttatt cctccaaaat catttctatg
    18481 aacgtagtat aacagaagag gtctgaaaaa caatctggta tgtcacatta actagtaaat
    18541 ttgtataaat agtaaaattt tggttgacat agcgattttt attgcataaa attcacttga
    18601 atacaaaaac taatatgtta gtttaggagg ggaaaaatga ttgatttaaa caactggagg
    18661 aactttatcc ctgaattatc tcaaaaagaa gtagaaatac ctcattttga ccgtgcatca
    18721 ttaagagaaa atggacaaaa aaatcctgtc tggatccatt tcggtggcgg aaatctttat
    18781 cgaggatttc atgcagaaat cgctcaacgt ttagcggatc taggcgaatt gtccactggt
    18841 gtcattgttt gtgaaacatt cgatgaagaa ataattgaaa gggcttacca agattttgat
    18901 gatttgattc ttgaagtggt catgccaagc agtggagagt tcgtcaaacg tatattagct
    18961 gcgacagctg acagtctgta ttgtcatcca gattgttctg aacactttga taagatgatt
    19021 cgacactttg aaaatcctag tttacaatta gttacttttg ctattactga aaaaggttat
    19081 cgattgaaag atacaaataa taaatatata ggaccgattg cagtcgatat aaaaaatggt
    19141 ccaaagaagc catcacatac aatgagtatt cttgctgtgc tactttataa acgtttttta
    19201 tctggagctt ggccgattgc acttgtctct acagataatt tttcacaaaa cggtagtcaa
    19261 ttacgtactt ctttgctaac gatcgcaaaa gaatggcaaa aggaaggtta tgtacaaaat
    19321 acatttatcc aatacttatc gaatgaagaa attgttgcgt ttccatggac gatgattgat
    19381 cgtatcaccc cgaatccttc cttgtcagtt agcaaaatgt tggcaaaaga gggcttttca
    19441 aatatgtcga ttttccaaac cgcaaaagga aataatattg ctccatttgt caatacagag
    19501 gacatccatt atttaatcat ggaagatcat tttcccaatg gacgtccgaa attcgaaaaa
    19561 gctggggtat tcttgactga tcgtgcaacg gtagataaag cagatacgat gaaggtgacg
    19621 acttgcctta atcctttgca tacgtcttta gctattatgg gttgtttact ggggtatagg
    19681 agcatagctg aggagatgaa agatccagat ttatcgctgc tagtttctga aatcgggtac
    19741 aatgaaggcc tcccagttgt ggagtctcca ggtatcattg atccaaagat ctttctggaa
    19801 gaagtaatta ctgtgcgctt ttctaatcca aatattccta atatgcctca gcgaatcgcc
    19861 acggatacct ctcaaaaggt ggcgattcgt tatggtgaga cgattaaaaa atatatggaa
    19921 atagaagtta aagacccgca gacacttgta ttcataccat tgactctcgc tgcttggctt
    19981 cgatacctta ttggcattga tgatcaagga aaaccattca tcccgagtcc agacccacta
    20041 ttgacagacc ttcagcataa gctttccgat attcaactgg gtacgcaaga tacatcacat
    20101 attcatgaga aaatggctcc tattttgtca aataaagaaa tttttggtgt agatctttac
    20161 caagcagagc ttggcagaaa aatagaagaa atgttcatca agatggtagc aggaccaaat
    20221 gccgtacgaa aaacattaca ggattactta caagaacatg gaggaaattt atcatggaaa
    20281 tgacttttag atggtacgga tcacaggaag aaaagattac cttagatgaa atcagacaga
    20341 ttcctgcggt caaagggatt gtaggcacat tgatggatat accagtagga gaggtatggc
    20401 ctagggagag gatcaaagaa ctaaaagaag aaattgagtc tcgtgggtta acactaaaag
    20461 taattgaaag cgtcaatgtg catgatgcca ttaaaattgg attaccttcc cgtgagcagt
    20521 atatcgaaaa ctataagcag acaatccgaa acttggcaga atatggaata gaagtcatct
    20581 gctataattt tatgcctgta tttgactggg tgaaatctga ccttgactac cgcttagaag
    20641 acggctcatc cacgttagct tttatatctg atgatattcc agatgatcca agagaaatcg
    20701 tcgcgcgcat cgaacattcc agcaatgaat ttggattgcc gggatgggaa cctgagcgct
    20761 tagctcctat caaatccttg cttgaagcct atgcatcagt agacgaagaa aaacttcgag
    20821 aaaattttgc ctattttctc caatcaatca tcccaacatg tgaagaggta ggggtcaaaa
    20881 tggctgtaca tccagatgat ccgccatact cgatttttgg attagctcga atcgtaaaga
    20941 atcgagaaga tttagattgg ttgtgtcgca tagtagacag cccttataac ggaatcactc
    21001 tttgtacagg gagtattgca gaagatccag aaaatgatgt atatgggatt ttagcagaat
    21061 tttgccaaag agatcgaatc ccatttgcac atgttcgaaa tattaaattt ttagctaatc
    21121 gagatttcta tgaggcacca cataaatccg aatacggttc attagacatg taccgcatac
    21181 taaaaacaat gtatgacaac ggatttaacg gatacatccg tcctgatcat ggaagaatga
    21241 tctggaatga gacaggaaga ccaggatacg gcttatatga tcgagcatta ggtgctgttt
    21301 atctaaatgg attatgggaa gcaatcgaaa aaggaaacaa aagtaaagat aattagtgaa
    21361 aaaaactgta attttattat gtaaataaga aggatgttca aattcttgat acataaatcc
    21421 ggaggttgtt aataagcatt cggatttatg tatcttattt ggtctaggaa gtaacttgat
    21481 tttctttttt cgattgatcc atgagtgctt cttcttttaa ttcaaagata tattctgaga
    21541 tcctcataat cacttttttc cagtcttctg gtgtgatgat tccttttaaa atatagattt
    21601 ttttgatatg agaaggcaat tgtttcaaag ggcagtcgct gataacgatg ttgcagttct
    21661 ccataaaatc attttgtatt ttgatcgatt catttgaaaa aatttgttct atcttaacga
    21721 tcagatcttt actgatataa aaatcacgcg tatagttcac accaatcaca attcgcggct
    21781 gttttttgta gcgatcatac aaatgtacta ggtcttcaac catccattgc acattttcct
    21841 tttctaaaat aactggtaac aagtgttgat tttgttctct gaaatccata taaaattgtt
    21901 caatggcggt ttggacttct ttcaatacgc tatgttcttg ataaagcatg aaagtaggaa
    21961 ggggcgtatt tggttgattg aagttaagaa atccattata aagtttattt cgtgcgaatg
    22021 ccaatgagta aagaatcttt tctttttcag gtataaacaa atcaaataca gtacaaaggg
    22081 actccaccat ttctcttgaa taaagaacat gaggaacgtt tgcttttttt aaaagtttgt
    22141 attgctccaa gcttgtttct tccgattcga ttttcgtgat agaaagtcga gctaaaatat
    22201 tcactaagat gcgttgttcg cgacaaattg gcagattagg tcgcaaaata tctacagaga
    22261 gatcaataaa aaagtcaata tattcatact ctgtgaattc tttttgtacg tctttcacag
    22321 aagttgcagg aaaattctct ttacatattt ttaatagaaa atataattta tctatcatac
    22381 cgctatttag tcggtcaagt acttcttttt taaaaaaagt attcatcata aaagccacta
    22441 cttgcggatc ttcatcataa aattctgtag cagtacttga aaagatgttc caataaatat
    22501 ctaatgctgc atattgaatt tgtacctctt ttccagaaaa agactttctg ccattattag
    22561 aaaaggagat agacaaaccg tactatgcta aaaatgcatt aatctttttc attttcgtat
    22621 acaaataaga ggcactatag tttgtttgct gactgaaact gataatcgaa tttgtctgac
    22681 agctgaacaa agaagtcaag atctgataac tgactgattc gttgcagtat cttccaaata
    22741 gacgataata gcagtaagcc ggatcaattc cttcaaaatt caactttaaa aaaggttttt
    22801 gcttatcaat atgcagttct gttagattga gacgctcaaa gtcttgttct aattctttga
    22861 tgtatagtaa aaaatttgat tcagacatat ggaaatgatc tgctaaatat tgtgtagtaa
    22921 taaagaaacg atgttcagat aggaaaagaa aaatatctaa ttttgtattg ctttttttct
    22981 tcaagataaa cgttttaatc gcttcttgag tttgcatgaa agaggctcct ttttacttaa
    23041 attgttaaaa cttgaaaata ttactgtttt tttcataaat ttagacaaaa aaacagtaaa
    23101 catataggta gaatctctac tatcttattt cttgcataca aaaaaatcaa ggagtgagag
    23161 agttatgaga tattcaacca tcttaatatc aacatttgca gccttaagct tatcgcttac
    23221 tataaatcag acagtccaca cagaggagac agaaacagcc aaatggacag aaaccaagtg
    23281 ggccaacgtc aatcgaatta cctttcagga tgaattttca tccttcaatt caggagtcgc
    23341 aaaattaaga aaaggacaaa cagcgaccat caaagcaaaa attgactatt cgggagactt
    23401 agcacctata ctacaatcag ctgttacatt tgagaatgtt gatcccactt tgtcgattgg
    23461 ctatgacgag aacactttga ctatccaaca caagcaagcg atatttacct tcaccgttac
    23521 attaaatcag gacttgttaa aaccagcgct ttttactgtc aaggtatcag atggactacc
    23581 gaacgatcat catttaacgc cgtatagtca gcggcagaca attgagcaag attcagccat
    23641 cgactcagga ggagatcttg ttgaagaacc aacagataaa ccagaaaatg aaaataagcc
    23701 agaagtaccg cctacagaaa atccagacgg agaacagaaa ccggaaatag aacctggaga
    23761 agaacccgat acagaaactc aaccagagcc agacaataaa tcaaaacctg aaataacccc
    23821 tggagaaaaa ccagatgtaa ctccagaacc agatacggat tctggaaacc aaacagtacc
    23881 ggaaacaaat ccagacacag ataatgaaac tgaaaatcca gaaaaaccag aggtagatcc
    23941 agaagaaaaa ccagatgtga ctccagaacc agatacggac gcaagagacc aaggaatacc
    24001 agaaaaaata aacaaaaaaa caattcaaga ggatggaaaa aaagagagta aaaaatcaaa
    24061 tctagcaatt ctaaaaataa atgaagagca gctgaataaa aaatcacgaa tatttgatag
    24121 cgcccaatca gctgaaacct taaaaagtag caaggataca acctttgctg tcctgaaacg
    24181 aaaaataaac agctaccaaa atccggagag tcacaaaaca aagttatact gtggagcgga
    24241 atcattctat taagtatagc aacaatgctt tctgcaaaaa gatttaaaca aaacagatct
    24301 ctctaacatt tgaataatgt ctgtgaacgc atttttcaaa taaaaagaaa tatccagaaa
    24361 atgaccccaa aataccagat tgaaatctga tatttggggt cgttcatact ttatctttct
    24421 tcatatttat tttaagggat atctgtttct tttggaagat ggttcgggat ctttttcact
    24481 ttcaatacat ctaaaaagaa ggtaaagatc ggaatgccga caatcaagcc ccaaacgcca
    24541 aagaaccgtt cgctaacaag taagaccaca aatgtataaa aaatcggcag ttctgttttg
    24601 ctggacatca acttcggatt cagcacataa gactcaatta gatggacaat caaaatcgta
    24661 atcaggatat aaacaacatc tcggatgcct ccttgggaat aagctatgaa agatagagga
    24721 atacaagaaa tgataacacc tgctactggg atcagactta aaatgaaaat catgatcgct
    24781 aaactcagca gctgatgaaa gccaaataca gccaaggcga ttgtagtgag caacgtattt
    24841 aaaagagcga caacaaattg agcttctaat accaaaccaa atgtattgac aaacttatcg
    24901 gcaaaatagt aaatatcttg aaagaaccaa gaaaattcac tttttaaaaa caatcttgag
    24961 aatacaattg tttttttctt ttcaatcata aaaaagaaac ttaagatgaa tgacatagca
    25021 aacgccactg ccatttttcc tatatcctgt aaatagccaa gcgcaatcga agcgccattt
    25081 tgaagctgtt caaaaaaatc gtttcgctca atataagtat ggatatattg taaaacagga
    25141 tctgcatctt ttggctgatg ctcgtaaaaa tcaataacgg actggatcat atcaaatgtt
    25201 tgatgaataa gcatcgggat atactttgtg aatgccagat agataaggta aacgatcagt
    25261 gtataaatca tgatagctaa aacacctgta gggattcgga caaatcgctg aatgatttga
    25321 acaagatgaa tcatcaaata agtaaagata aatgtgagca aaatggttgt aatcatactt
    25381 ctaactaaga aaagaacaaa aacgacaaat aataaaacag tgagacggcg tattttttca
    25441 ttcgccaaaa aacgctgata aacggtcaaa tcgacaacct ccaaataaca aaaaataaac
    25501 acgttattca ttatatgatg gaatatgaaa ataagaaaga gaaagagcaa aaagaaaaac
    25561 cgctaaatga cgttatctta tgagttggtt ttcctcataa aatcgttcat ctagcagttt
    25621 cagccctttt tctggtcttg atgaaaatta tctagcattt ttcgggtcag atgttgcagt
    25681 gtatccaatt cttcctgttc aagtggaaga taagacttga tcttttgtgg aataccttct
    25741 gcttgttttt gcaacgcagc accttgagga gtcagcgtaa catttacgat tctttcatca
    25801 cccgtttcac gctgacgatt aatataattt ttttctgcta acttttttag taagggagtc
    25861 agcgtaccag aatccagaca caaatcattt cctagctctt tcactgaagt ctttttcttt
    25921 tcccataaaa ctaacatcac aagatactgg gtataggtaa gattcagcgg cttgaggagg
    25981 gaagtgtagt gtctaatgat ttccttagag gcggcatata aaggaaaaca tacttgatta
    26041 actaaatata gatcttccat ttttccacct ccgctgtttt actatagtgt atcatagcgg
    26101 atttacacaa aaaagtctat tcactaactg ctttcagagt aacttctata tttccagctg
    26161 tagcttttga atacggacac actttatgcg ctgcctctaa taattcttgc gtttcttcca
    26221 gagaaagacc ttcgatagat ccttcaatct ctacacctaa gacaaagctc gtttcacttt
    26281 ctgaataaag ggaaacttgt gcactgacag tagaagcccc agagatattt ttttgctgtt
    26341 tgatcaattc taatgcactg ttaaaacatg cactgaaacc agctgcaaac aattgttcag
    26401 ggttggttgc attttcgacg cgtttaccag gagctgcaat attcaattcg aatgtacgat
    26461 cgggattatg aacttctcca gagcgtccgc ctgtgttgat cattttcgta gaatacattt
    26521 ttttcataaa attaccacct ttaattatat tttatacaat tgaattttac acaatataat
    26581 tgtggaaagc aaagaaatgg attggcataa gataaataag agaaagcaca tgaaacagac
    26641 gcgctttctc gaatagcaaa catatacaaa aaaatatcag agacaaaaac taatctcatc
    26701 ataaacatta catatatttc tcaaaaagtt gttttctttc ttcaaaactt acttcacgat
    26761 ggacctgatg aagcatccat aaataaccaa aaggatctct gaacattgag ttagtcacac
    26821 caaagtccat catttctgtc atttcttgaa tctctacaca gccatttcgt aatgctttat
    26881 tatgtgtgtc ctcgatatca gagacgatga tattgaacca gatcgtattt ggatgttcag
    26941 cagtaggagc agtcaaacca agagacgtgt tttcatccag catatggaat ctcgtgccat
    27001 acaaggtaaa aatgacttca ttattgcctc tagggtaatc tgttgcctca atcagttgta
    27061 tttcatcaaa gattgtttga tacagttcta gtgctttcaa actatcattt aatacaaaat
    27121 ctatttccgt tcctttatgc ataaagatct cctctccttt tcagtcgaac agttactaga
    27181 ttcttttatt ataccagtta aagaaaacgc attcaaaaaa atagcggatt tacttaccaa
    27241 ctcattcgat tggatgataa gtaaatccgc gtcccattac ttctgctacg ctgctgacaa
    27301 tgacaaaggc acttggatcg atttcttcta tcaatggttt cattcgggaa aattcatgat
    27361 cagtcacaac gatcaataaa acctcttttg actttcgatt atacccgcct tttgcctcta
    27421 gcagggtaag gccacgtcca atctcagcac ttaagcgaag gcggatgtct tccaaataat
    27481 gttcactcat gaccataaca gatttctttc gattgatacc tgtttcgata taagtcatga
    27541 caattgaagt gattgcaata gataaaatag cataaagaaa ctcttctatt ccaaaaacaa
    27601 acagattaag agaaacaacg aatgcgtctg taaaaaataa gccaatcgaa gtattcaagt
    27661 ggaaatattt tttaaaaatg aggggaggga tcgtcgtacc gccgctagaa gagttgtttt
    27721 tatataaaat cgcaactcct aaagcaaaaa tcgcacttcc aaaaatgaca gacaacaacc
    27781 gatctgaagt aatcattgtt tctggaacta ttgcaatgat taaaggaaaa aacaagcttc
    27841 cgaaaagtac tttaaaaaag ggttttttac ctaaaaatag aagagctagt accaacataa
    27901 cgatattcaa cactaaaata actatagctc ggtcaaatcc taaaacagct tccagtaata
    27961 ttcctagacc gctgacacct ccagcagcaa tatgatgagg ccctaaaaac atattgatag
    28021 cgactcctaa tatgaagagg gcaaaaataa tcttaatgta tgtccatata aattgtctcg
    28081 tttctttcat ttatccactt ccttaattca ttaataatac tcttatctta ttataactta
    28141 cttttcacct taagttaact ttaattaata aaaaaattag gctagtctaa attattatgt
    28201 ttcattcttc tagaatatac ttctacgtta aaaactgtta aaaaactttt actttagaaa
    28261 attggagaaa ttgatcgctt aagataaatt caggacacta aatagtacag gaagtagatt
    28321 aaaactttta aaagttttaa gtctatctat ttggcaagtt taaaaaataa aaattcggta
    28381 tcaaaaaact gttaccgata acatttttga aagcgttgtc ttttgtttct ctatcactta
    28441 tagtgatgac gtcatattaa aacacaagga ggaaatcaat ggatacagct gcaatttatc
    28501 atcgaccgga aagtgaatat gcatttctat atacgaatac aagatttcgt attcggcttc
    28561 gtactaggaa aaatgatatc aaaaaagtat atgtgctttg tggagatcca tataccataa
    28621 caactgaaaa atggtatcaa aaacaacgac caatgaaaaa atgtctaagt accaatgttc
    28681 atgattattg ggaaattgaa gtgacgagcg aaacaaggcg cttacagtat gcgttccatg
    28741 ttgttgggga agataacacg gactgttttt acggtgatca aggaattttt ccttatcgag
    28801 aaaatgtgat tactgaacca aatttttatt ttcgaatacc ttattttcat caaatcgatc
    28861 gattcactat tccagaatgg gtaaaagaaa cagtatggta tcaaattttt cccgaaagat
    28921 ttgcaaacgg ggacaaagca aatgaccccg aacacacttt accttgggga agtaaagatc
    28981 cagaccgaga agacttcttc gggggagatt tgcaaggtgt gagggatcat ttggattact
    29041 tgacggacct tggcgtcaat ggcatctatt tttgtcccat atttaaagcc acatccaatc
    29101 ataaatacga tacgattgat tattatgagg tggatcccgc ttttggcgat aaaaaacttt
    29161 taaaaaactt gattgatgaa gcacataaaa gagggatacg tatcatgttc gatgctgtat
    29221 tcaatcatat gggtgtccat tcaccacagt ggcaagacgt tctcaaaaac ggagaaaaat
    29281 cgatctataa agactggttc cacatccgtt ttttcccagt agattcttat cagatgactg
    29341 acttaccaga aactgctgaa aatatcccat atgatacttt tgcttttact ccattcatgc
    29401 cgaaactaaa tacagcaaat ccagaagtgc aaaaatattt gctggatatc gcaacttatt
    29461 ggataaaaga attcgatatt gatggatggc gtcttgatgt tgctaatgaa gtcgatcatc
    29521 atttttggaa aaaattcaga gaagcagtta cagaaatcaa accagacatc tatatccttg
    29581 gagagatttg gcattcctct caagcttggc tgcaagggga tgaattccat gcagtgatga
    29641 attatgcatt cacagattcg attaaagatt attttgcaaa aaagaaaatc acagcttcac
    29701 aaatggtcag tggaatgaat catcagcaaa tgctttacag agatcaggtg aacgaaggaa
    29761 cattcaactt attagattct catgatactg ccaggatctt gaccctttgc caaggtaaca
    29821 aagaattgat gaagtctgtg atggccttta tgtttcttca aaaagggtct ccttgtatct
    29881 attacgggac agaaataggg atgactggtg aagatgaccc ggattgccgc aaatgtatga
    29941 tatgggagaa ggaggaacaa gatctagagc tattcggttt tataaaagaa cttgtttctc
    30001 taaggaaaca gttgtctaag attatttctg aaggttctac tcagtggcta atagtatatg
    30061 atcgtgaaga taagctctat ttcacacggg aactagaggg acagatcatc tatgtttact
    30121 ttaatcagtc aaaagaacct tgggtagtag aacaggaaaa tgaggtgatt cttagtcaaa
    30181 attgtcagtt gttagaagac gggaaagcag agatacgaca atacggattt ttgatcatgt
    30241 gttgaaaact gttaccggta acaacaagaa agcgtttgca aatgaagatt tgtgcccatt
    30301 ataataaggg tgtaacagca cgttgaatac acaggaggaa atgaagatga aaaaaagaag
    30361 tgtgaaacag ttgggtttag ggttaatctc tttaggaatc ttaggcggat tagcggcttg
    30421 tggcaatggt aactcttcag cagaagggga agccgatgaa ggaaaaacgt taacagtttc
    30481 cgttgattct ggatataaag attatataaa cgaaataaaa aacactttcg aaaaagaaaa
    30541 cgatgtgaaa atcaagctag tagaaaagga catgttcgat cagttggaat cattagcgtt
    30601 agatgggcct gctggaaagg gacctgacgt tatgatggca gcatatgacc ggatcggtgc
    30661 gttaggacag caaggacatc tggcggaagt aaaactggga aatgaagcag cttacgatga
    30721 aacagacaaa gcccaagtga cttatgatgg aaaaatctac ggagaacctg cagtaatcga
    30781 gacccttgtt ttgtactata acaaagattt agtcgataca gcaccatcaa cgtttaaaga
    30841 tttggagaat ctatccaaag acagtcgttt tgcttttgag tcagaagcag gaaaaaatac
    30901 aggattttta gctaaatgga ctgactttta ttattcctat ggtttaattg caggttacgg
    30961 tggctatgta tttggtgatg atggcacaaa tccttcagat atcggtttga ataatgcagg
    31021 tgctgtagaa ggaatctctt atgcaacaga ttggttcaaa aatgtttggc caaagggaat
    31081 gcaagacata aaaagtgcag gagacttcgc cagccagcaa ttcatgtcca ataaaactgc
    31141 agcaatcatt gacggtccat ggcaagcgca aacatacaaa gaaaacaata tcaattatgg
    31201 tgtagccaaa attcctactc taaataatgg acagccttat caaccgtttg gcggaggaaa
    31261 aggatgggta gtcagcaatt atgctaaaaa caaagacctc tctcaaaaat ggcttgatta
    31321 tgtaacgaat caggaaaatc aagaaaaatt ctttgaaatg gtaaatgaaa tccctgcaaa
    31381 ccaacaagct agggaaacag caaaggctaa aaacgatgaa ttaacaacag ctgtgattga
    31441 acaatatgaa acagctcaag cgatgccaaa catcccagaa atgggcgaag tttggacagg
    31501 tgcagaaaac ttgatgttcg atgcagcatc aggttcaaaa acaccaaaag aatccgcaga
    31561 tgaagcagta aaaacaatca gtgaagccat agaacaaaaa tactgattaa aatgataacc
    31621 gtccattttt ataaaaaatg gaacaggtgc ttcagataaa agtggcaaga gcgagatgga
    31681 aaattatctt gctcttgttg ccattatctt aaaagcagtt tgcggtttag caagtttaga
    31741 gggggcattc agatgcagca agataaaaca agcaatatca acgaaaaaaa aatcacgctt
    31801 tgttcactta ttccagggat ggggcagttt gtaaatggcc aagcttttaa aggtatcgtt
    31861 tttttcgcca tattcgtcct attcattggt cagatgcttc tgggcggatt tcatgcattg
    31921 gtaggattgg ttactttggg aagtatacca atggaagatc attcattatt tattttgatt
    31981 gaaggcactt tgcaattcat cattacaatc attttcttga ttttttacgg tttgaatctt
    32041 ttagacgctt atcgagtagc gaagatgcgt caagagggca aaaaaatcaa tcggacgatg
    32101 aaggaagtca tacataatat aggtgatgaa ggttttcctt atctattgac acttcccgct
    32161 tatcttctca tgatttttac gatcattttc ccagtcctag ttacattgtt cactgcattt
    32221 actaattatg acttcaatca tattccacca gccagtttaa ttgattgggt aggggctaaa
    32281 aactttttta gtatcttctt tttaagctca tatcgtaata ctttttttgc agtcttttcg
    32341 tggaccgtga tttggacttt atgtgcaaca actttgcaaa tcacccttgg tattctcaca
    32401 gcggttgttg ctcaccagaa atttattaaa ttcaaacgta ttttcggtat cattttctta
    32461 ttaccatggg cggtcccagc ttttattact atcatgagtt tttcgaatat attcaacgat
    32521 agcgtaggtg ccatcaatac tcaagtgatt cctttcataa accagcttcc ttttgtagat
    32581 atcagtgcaa tctcttggaa aacggacccg aactggacga aagtagcaat tatcttagtg
    32641 cagggctggt taggatttcc ctatatctat gtgatggtta caggaatctt acaatcgatt
    32701 tcagaagact tatatgaagc agcaaaaatc gacggagcaa atgcatggca gcgcttttca
    32761 aacatcacgc ttcctgctgt cttcctagtg gcagcgccaa catttattac acagtatacg
    32821 gggaatttca ataatttctc catgatctat ctgtttaaca atggtggtcc cggaagcgta
    32881 ggcggaggag ctggttctac tgatattctt atttcctgga tctataaact tacgactgga
    32941 acttctccgc agtattctat ggcttcagca atcacgttga ttatctccgt catcgttatc
    33001 tgtgtgtcat tgctggtatt taaaaagaca aatgcgttca atatggggga ttaacaatga
    33061 aaaaatttca ttcgagtgct cgattccaaa aacgactttc tcactttttt acttactttt
    33121 ttcttttaat actgtcagtc attatcatct atccgctttt gatcacagca agtactgcct
    33181 tcaagtcagg aaatattgca gcttttagtt tagatctttc aagtgactgg actttaggaa
    33241 actttactcg gctattcagt gaaacacttt acgggacttg gtataaaaat acattgatca
    33301 tttctgtcct aacaatgatc gtacaagtga ctgtagtaac tttggcgggc tatacttata
    33361 gtcgctatcg ttttatcggc cgcaaatcaa gtttgatgtt ctttttagtg attcagatgg
    33421 taccgactat ggcagcattg actgcctttt atgtcatggc tttcttatta gaagcgctcg
    33481 ataagtactg gttcttgaca atgatctata taggcggtgg aattccaatg aatacttggc
    33541 tgatgaaagg atactttgac actgtaccaa tagatttaga tgaatctgct aaacttgatg
    33601 gggcagggca tttcaggatt tttttccaga ttgtattacc gcttgtcaaa ccgatgctcg
    33661 ccgttcaagc attatgggct tttatgggac cttttgggga tttcatgtta gctaagttcc
    33721 tgcttcgaac acctgaaaat cagactgttg ctgtcggctt acaaacattt atttctgatg
    33781 tgacaaacca aagagtctct ctttttgctg ctggggcaat tttgattgca ctaccgattt
    33841 gtctattatt cttcttttta caaaagaact ttgttaccgg cctgacatct ggcggaacaa
    33901 aaggatagga gcagagtatc atgcaaacat ttccgttaaa ctatttttcc gcattacgta
    33961 cgccaactca agtgtttgcg ggacgcaaac tattatcatg gcctaaattt ttcttgatat
    34021 ttgtattttt agtttccttg atggtcatgc ctgtgactct tttttatgcc aatcagatcc
    34081 aagcgattcc attagaacaa tttctatccg ttcattcgct gatcgatgaa caaggaaccc
    34141 aaaaattttc tgaacttgaa ttatctgaaa ctgggttaca agcatcgcaa caaacgatag
    34201 ctgtgacccc tgaaattttg gtaggtgtgt ctttatctga gaaacaacaa tccgatcatg
    34261 gaacttttat tgattttgag aaggaacaat gggtgatcca acaaaaggat aaatctggta
    34321 tacgccgcta tacgatgaat tatagcccat ctttccaacc agattctgta cgtacgcccg
    34381 aagatttcca acgattttta gaaagagaat tttatgccag taatcgtcca acaatcattt
    34441 tatcttatag tttaagtctt ggtcttgtcc tatttgtcat gacgagtctc attttattcg
    34501 gggcttcctt tttcttgtgg atgacaagaa aaagccaact ttcatccatt catacattca
    34561 aagagagtgc aaatttgatg ctgaatgtta tgggaatagg ttccatggca gcagcagttg
    34621 tagggtttat ccactttgac tttatcttga tgctaagtgt ccaaacaacg ataactgtct
    34681 tgctgctttt atgggttttt gtcaaaacaa gattcaaaga taagcgtgtc gaatgacaag
    34741 tttaagcgat ttatgcgaca atgaatgtga aaaaaatgaa aaataaagta gtttttgaaa
    34801 aatttctgaa aaaattaaac ttcttagttg attcttttct tttttagtat taacgtaata
    34861 gggtagtttt tttaaggagg aaaatcatgt tagagaaaac tttatacaat caattatttt
    34921 cacgttcttt ctcgctccct gtcgaagtaa attattggga cggtacaacg aaacaatacg
    34981 gagatacgga cactcctccc caaatcaaaa tcaatatcca tgaagaaatc ccaatgaaag
    35041 agattacaag taacgcttct ttggcactag gagaagccta tatggatcat cgaatcgaga
    35101 tcgagggaag tatccaagca ttgatcaacg atgcttatca gcaagcaaac agctttttac
    35161 gaggaaaaga ttatctaaaa tggctgccaa agaaagaaaa acatacgaaa caaaaaaaca
    35221 aagaagatat ccatgcgcat tatgacttag gtaacgactt ttataaactt tggctagatc
    35281 caactttgac ttattcttgt gcttatttca atacacctga agatacgttg gaacaagcac
    35341 aaatcaataa agtccatcat atcttagaca aactctttat caaagaaggc gaaacgttgc
    35401 ttgatatcgg ttgtggctgg ggaacattga tgttcactgc taccaaagag tataatgtca
    35461 aggcaactgg gatcacacta agtgaagaac aatacgattt gattaccaaa agaataaaag
    35521 aagaacattt agaagataaa tgccgtgtat tattaatgga ttaccgcgaa ctaaaaggcg
    35581 aaacgtttga tcatatcaca agtgtaggta tgttcgaaca tgtaggttct gaaaatcttg
    35641 aaggttattt caaagtggta aaagatttat tgaaaccaaa aggaacagct ttgatccatg
    35701 gaatcagccg acaacaaggc ggtgcgacaa atgcttggat caataaatat attttccctg
    35761 gcggttatgt ccctggtgtt actgaattag ttgataatat tacaaaaaac gatttgcaac
    35821 tgattgattt ggaaagctta cgtcgtgact accaattgac attagaacat tggacaaaga
    35881 attttcatca agtccaagat caagtcacga aagaaatggg tgattccttc tatcgaatgt
    35941 gggatctata tttacaagct tgtgcagctt catttgaatc aagtaatatc gatgtgattc
    36001 aatatttgct tgttcaacct ggaaacaatg acatcccaat gcatcgtgca taattgaggc
    36061 aaaagtactg tttaaagact atttaaaaag aagctaagtt ttatgatgct ccagaagtga
    36121 acttttttgt ctaacttcta ggattgtctc taaagcttag tttctttttc tttatatttt
    36181 cataataaaa gtttggttct ttaattttta aacatgacag aaaatttttt cttcgttata
    36241 cttgtaatag aatactattt tataacaaag gggatcgaat caatgaaaaa aactgttatt
    36301 tatactagtt tatttgcagc tttgttttta gctgcttgtc aatcaaatac caatcaaggc
    36361 acatctggga atacgtcttc cagtagcgaa cagcgctctt cacaggtatc ggactcctca
    36421 acaaaagcaa gcacatcttc gtctagccag acaaaaagtt ctagtgaagc aagtactcag
    36481 tcagatatca aagtgtctgc agctcgtgcg atcgaattgt tccaagataa atacccagat
    36541 actgcaatta ctagtttgga attagatagt gattggggat cttacttcta caagatagaa
    36601 ggtgtcgatg atcaaaaaga atacgaagca agaattgatg ctcaaaatga aaaaatggag
    36661 acagagaatc cagaaactct agatcgagat gaacaaaatg gtcaaaagaa aaaagaagat
    36721 ggtctggatg ttgataaatt gatcacgatt gaggaagcag caaaaatcgc cgtcgacaaa
    36781 gtaggaagcg gtacggcaac agattgggat ttggataaag atttaggtac tacttattgg
    36841 gaagtcaaag taaaagacgg caatcaaagt acatcagtca agatcaatag ccaaacaggc
    36901 gaagtactgt caactgaaca agatgattaa gaatttcttc ggaaaacaag agttgatttt
    36961 tcttttagtc atgatacact agacaaaata acttgtttga aggagcgatt tacttgagtt
    37021 tagaaacgac ctttgccaat catatctttg ccaatccttt gatgaatgca tctggtgtcc
    37081 actgcatgac tacccaggaa ttagatgaac tggctcattc agaagcagga gcatttatca
    37141 ctaagagctg tacgatcaat gaacgaaaag gaaatcccga accacgctat tttgatgttc
    37201 ctttagggag tatcaattct atgggacttc caaacttagg tttttcttat tatctagagt
    37261 atgctcttgc gtatgaaaaa gtgcaggaaa accaaaatca accgttattt ttttccattg
    37321 ccgggatgag tgtccaggaa aacttggaaa tgcttgagaa aatagaaaaa agtgggttta
    37381 atgggatcac tgaattaaat ttatcttgtc ccaatgtgcc tggaaaacca caactagctt
    37441 acgattttga ggccacttat gaaacactga aagaagtatt cagcatcttt tctaagccgc
    37501 taggaatcaa attgcctcct tattttgatt ttgctcattt tgatcagatg gctgatatct
    37561 tgaatcagtt tccattgacc tatgtcaatg ctatcaacag tgtgggcaat ggtttgtata
    37621 tcgatacaga acaagaagca gtagtcatca aaccaaaaga agggttcggc ggcattggcg
    37681 gagagtatat caagccgact gctttggcga atgttcgcgc tttttataca cgtttgaaac
    37741 cagaaatcca aatcattggt acaggtggga ttcggacagg acaagatgca tttgaacatc
    37801 tgctatgtgg cgcaagcatg ttacagattg gtacagagct tcataaagaa gggccggaaa
    37861 ttttttctag aatcataaaa gaattaacgc agattatgtc agaaaaagga tatacgtcaa
    37921 ttgacgaatt taaaggcaaa ttacgcacca tttcatagca aagagacgaa ggtcatgatt
    37981 gatcttcgtc tttttttata aaaaacactc gtctgttcat tcctgtgcaa gcgtactcat
    38041 tgtatgttag acgaacttgt gggaaaataa cagaggcaaa aaggaggata atatgaatga
    38101 tgcaacagaa ttagcaaaag agcttaaaga aaaagaattt agttttcaag aactagttac
    38161 ggatatacag aaaaaaataa agaagcaaaa tcatgaattg aatgcgtttg tcgcgcttga
    38221 accattcgag agtaattccc acgagctatt gattccccag tcagaaaaag ggccattggc
    38281 tggtatccca tttcctttaa aaatgctcgg tcaagaaaaa aagggatggc tagctacttc
    38341 aggatctcgt ttatttgaaa cacaccgagc ttctcataca tctaattatg tgcggcaagc
    38401 agaagcgata ggtctagttc cttttggaca aaccaatgcg ccagagtttg gtttcaaaaa
    38461 tattacagac ccagtcatct atggcccagc aagaaaccct tggaaccttg aatgtacacc
    38521 gggcggatca agtggaggag ctgctgctgc agtttcatca ggaatcgttc ctttagctgg
    38581 tgctagtgat ggcggtggtt ctattagaat acccgcttcg ttttgcggat tgattgggct
    38641 gaaaccttcg cgagggacga tgccagttgg tccatatgct tggagaggat ggcaaggagc
    38701 tgctattgat ttcggcttga cagtttctat gagagacacg gaggctttat tttatgggat
    38761 gagaagtatc cattccggtg cgccatatca agtatcgcca gttgagtggc aaacacatcc
    38821 aagaaaaaaa cggttgaaga tagctttttg tgttgattca ccgatccact cacagattag
    38881 tgaagaggca gtacttgctg taaaaaaagc tgtagatttc cttggaaaag aagggcatga
    38941 tttgacagag attccttacc cactagatgg tcgtaaactg atcacgtctt attatcagat
    39001 gaatggcgca gaaacagcag caatgatccg ctcgattgaa acaggcatcc agcgagctgt
    39061 cagaaaagaa gaacttgaac ccatttcttg gactttgttc caatatggtc agaagctctc
    39121 tgccgctact tacatcgatt ctctgcatgt atgggatgaa gcagcaattg tcatggaaaa
    39181 cctacatcaa gaatacgatc tattcctttc accaacgact gcatttaccg cccctaaaat
    39241 cagcgaagat ttgcaaagtg atgcgataag agcacagatg gaacacatca gtgattgttc
    39301 tgaagtagaa ggtttagacg ttgtttcttc catgtttgag gagagcttga agatcacacc
    39361 ttatacacag ttggctaatc tgactggaca accagcaatc agcttgccta ctcatttgac
    39421 aaaagaaaac atgcctctag gaatccaatt tatggctgcc agaggaagag aagatttgct
    39481 atttcaaatc ggctatctat ttgaagcaaa tcatcagttc catttaccaa atccatggaa
    39541 atagcagaaa caaggagaag aagaatgaag aaaaaaagat tgtttgctct aataactagt
    39601 gcctttttta ttttaagcgc ttgcggtaca gctgccgcta atcaggaaga taacagctgg
    39661 agtaatgtag aaaaaaagaa agaattagta gtagcgactt ctggcacact gtttcccgca
    39721 tcttattata atgatcaaaa tgaattagtt ggttatgatg tagatttggc aaaagaggtg
    39781 gctaaacggt taaatgtcaa aatctccttt aaagaataca acgtggatgg acaaatcact
    39841 tctgtttcaa aaagagaatc agattttgct gcgaacgatt ttggtatcac aaaagaaaga
    39901 gaaaaaaaat tctccctttc agagactttc aaatattcat ttgacagtat gatcgtacga
    39961 aaaaaagatg attcaggaat ccactcactt gaagatctta aagggaaaaa agcagctggc
    40021 gaacccaata ctagctatat gcgtttagcg gaaaaacacg gagctaaatt agtcacatat
    40081 gacaatgcca cgaatgatca gtatttaagc gatgtagcaa atggtcgaac agatgttatt
    40141 ctaaatgatt attatctgca gaaaatggcc gtatctgctt taccagatat ccctgtaaaa
    40201 atactggaga acgtttattt caatgaaaac gaaagtggtt ttttattgaa agaaggaaat
    40261 gatgctctga aaaagaaaat cgatggcgta ttaaaagaga tgaaagaaga tgggacgaca
    40321 aagaaaatat ctgaaaaata ttttcaaact gatgtttcag tcaaaccgaa agaaacgatg
    40381 gctgagtcgg tgagtgagtg atgtactttc ccctaattga tttttccctc atgtggactt
    40441 cacttccttt cgtactgcaa ggcttggtat acacattagg gattggcttc gtgtcgtttc
    40501 ttttaggaaa tttggtaggg ttgcttttga cagttttagg tcttttggat tggctgcctc
    40561 tgaatgtatt tattcgtttt tatttatcgt tctttagagg cattcccgca ctagtcctgc
    40621 tgtttctgtt atatttcggg ttaccttatc aattaagtgc cttaacggct tcagtcatct
    40681 gctttacgat taccagcagt gcatttattg gtgaaatcta tcgaggatca ctagcaggcg
    40741 tttcttcagg tcagtgggac gctggtttag cttctggttt ttctttcttt ggggtattga
    40801 aatatatcgt tttgccgcaa gcttttcgaa tctcagtacc ggcactcagt aacgtagcta
    40861 tggatctagt gaaaggtact tcgttagcag caacaatcac agtgccagaa atatttcaaa
    40921 aagcaaagat cataggcggt cggacattcg attatttttc tctctatatt ttagttgcac
    40981 tgatttattg ggtcatttgt atcgggatcg aaaaagtgca acagtattta gaaaaacgtt
    41041 ctatccatca gtcaatctga aaaatagaaa tggtgctagc gtggctggca ccatttctat
    41101 tttttgtttc ttgtatattc tgcctagtat tttaaaacca tttcatgtaa aatgtaaatc
    41161 gaaaatatgt tcggaggtag aaaacatgaa gaccccgcta accatcgaaa tagaatcctc
    41221 gttataccaa tattgcatcg agcaaggcgg cttggttgta gaagaagtca cgatgcctga
    41281 tgaccaagga attgtagata cgcttagctg cttgacaaaa tttgatggaa gccgagaatg
    41341 gcgatgctat gagctgaaag tgacaaaagc agattttcgc tctacagcca aactgtcatt
    41401 tgttggacat tataattatt ttgttttacc aagtaaacta tatgaagaag taaaaactga
    41461 aatccctaca ggaataggtg ttttagtgta tcgtccgtac acggttgaag aggagatgcc
    41521 tgcctctggt acatttctga ttgcgaaaaa agcagcaaag aaagagcttg ctgtaccgga
    41581 agaagctttg acaaaccgct tcatggcttc tttattcagg gaagtaagaa aagcaaagca
    41641 aatggagcga ggcgtaaaat attttccaaa agatcaattg tacaaagaat tgaaaagacg
    41701 ttcagaaaat caagatccat tttctaacga acgatattat gaacggtttt tagaggaaat
    41761 agctagtcag gcaatcaatg agttgcaaga agaagttatc gcgcttcagc atgattatga
    41821 atatttgagg caacaacagc agatccgtcg gttgcctaca gagccattgg agtgaaaaat
    41881 atgtactatc cttatttaag aggaaaacaa tttgacctat tagcattaaa agaagcactg
    41941 tcgcgaggat tgctctccaa taaaatccaa ccagtgatcg aaccagtacg tgattccgct
    42001 acgcttaaaa atgtgatcga attatttcaa aaaaagcacc atccaatcgc tgtgatccaa
    42061 aatccccaag taggacaatt caaattattc gatcagcatg tgcacagttg gacagtcaaa
    42121 gaaaatagct ctgtcgtccc tgcacagatc ttaacgccag aaaatctcag tgaagtactg
    42181 gattcacctc cagcgtttct tgttttcgat ggacagcact acccaaaaga tacagaggtg
    42241 tggaaacagt tggcaggagt tgattcgaaa tttttgatac cagatactag tcgctttcgc
    42301 atctggctgc ccgaaaacaa aattgtgatc cgtgactctt ttcaaacgag aaagcatgtt
    42361 gaaagttatg cagataaaac agatgacttt tttagtgatg actatctttt tttccacgca
    42421 gacggatata ttggattttc tgatttcacg atcgaaggta gccgttattt cgataaaggg
    42481 ttccctagcc gagcgcttgc tatccatcta acgtatattg atgcttacgg caatatacgc
    42541 gtgaaacatt ttgtctctga cagtaacgac tctgctaaag accaagcatt gaagtttctt
    42601 gaagcaggag ataaaatgaa aaaatggatc atgcgtcacc atcatcaact cttgattact
    42661 tcaggaatga ttgaattatt ggctctttac cagcaacaaa aattccctgg actgggagtg
    42721 ttgaaaaagt ggtcacttat gcatcatttg gaacttatta gtcacctgct agatcatcca
    42781 aaagattggc ttaaatatta ttcaaaagtt caggaactat atgaagtgat ccaatgatta
    42841 gataatgtaa atgaaaaagg aatagaaaag aggaaactgg atgtatattc ggcaaacaag
    42901 gaaagaagaa gtagaagaag tggtagaaat cattaatgcc gcagcggatt tcattgcttc
    42961 gcaaggctct cctcaatggc aaaacgggca gaaaccaaca aaagaaacga ttttgaacga
    43021 tattgaaaga aaagaaagct atgtattaat agtggaagat caaattgctg gaacagctgc
    43081 attagtctca ggcgtggatc ccgtttacac aaaaatagat ggtagttgga aagaatcaac
    43141 tggtccttat ttatcgattc atcgtgtcgc tttagcagaa agtactcgag gaaaaaaact
    43201 agcaaaaccg ttcttagaac atttgttgac gattgcagcc agcaaaaata tccaagatat
    43261 ccgtatcgat acttatccag ctaatgaacc gatggaaaag accatctatt ctgctggatt
    43321 cacttatcaa gggatgattg aatttccttt tgaacacggc gaaagaaaag catatcaaat
    43381 aatcatcaat tgactataaa atcatctgag caaagataaa gacattaaca ctactgaaaa
    43441 aaacaatcat atgatagcca agaggttgta acagaagtgg ccagcttcaa aaaacaagaa
    43501 agaatttccg aaaatgcttc tcaaattttt gtggaatttt agcgtatttt acgaaatagc
    43561 tgctttctga aactcccttc atcgagaggt cgtgacatag cttttgtcac gatctctcgg
    43621 cttatcctac gagtattatt cgatacattt atatcaataa tccttttttt attttatctg
    43681 cagcttcttg gaacgctcca tcgtttgaca cgactaaaag tgaggggata gctttcaaac
    43741 gtttcaactg tgctaaatct tctacataaa ccgccagtct ggctgaaatc tgttcagatg
    43801 aatctccacg ttttttcagg cgcgattgca atgtttcttt agagatcgta ataaagatag
    43861 gacggactac cgtcccgaaa tactcatata aatgccaaaa accatttgct gtcaaaacgg
    43921 cgtaacaatt tcctactgtc agtttctttt taatttctgc tttactggaa ccatatttat
    43981 gttgagcata ttggtcccac tctgcaaact catttccctt gatcatctga ttgaatgtat
    44041 tgtcagaaac aaaataataa tcctcttgat ctttttcccc aggtcttggt gatcgattcg
    44101 tgtaagtaat gatcttttgt tccggctgaa aaatagcttc tgcgagtgaa gtttttccag
    44161 aaccagaagg accgataaat acatagcaat aattcattgt catttctcct ttgatgattg
    44221 aacaagtgaa aaagctttgg aaaatatttc caaagctttt aaaagtaact ttgctttttt
    44281 gtattttata ccagcaagcc agtcacaata gcacttaaaa agctgactaa agtcgctgtt
    44341 agcaaaattt tcaatccgtg ttttgcgaca agatttcctt tttcttcatt taatcccttc
    44401 atcgcaccag aaatgatccc gatagaagag aagttggcaa atgaaacgag gaagacagat
    44461 ataattgctg ttgtacgctc agtaaaatgg aaattaccat ttgctaattc tgtcatagca
    44521 acaaattcat ttgtaaccag tttagtcgcc atgatactac cggcatccac tgcttctttc
    44581 catgggattc cgctgatgaa ggcaagaggg gcaaagatgt aacctaggat ctcttggaaa
    44641 gtgatgccga atatcccgtg gaagatagca ttgatcatgg caatcaaagc aacaaaaccg
    44701 ataagcatag cagcaaccgt gatcgccaca tggaaaccat caagaatgta ttctcccaac
    44761 atttggaaaa atgtctgttc tttgttttct tcaatgatca gctcatcttc tttttcttct
    44821 aatacatagg gatttacaat tgaagccaga atataaccgc caaataagtt caaaacaagg
    44881 gcagtgatca catatttaga atcgatcagt gccatataag atccgacgat cgacatagaa
    44941 actgtagaca tagccgatac actcatggtg aacaaacgtt tctcagatag gaagggtaat
    45001 tcctttttta tggaaataaa aacttccgat tgtcctaaaa tagcagaagc tactccatta
    45061 taagattcca atttgcccat cccgttgatt ttacttaatc ctagaccaac agctttcata
    45121 aataatggaa gaatcttgat atagcgtaga ataccgataa tagcagaaat aaatacaatt
    45181 ggcatcaata cggaaagaaa gaaaggtgca cttcctttat ttgctacacc ggcaaaaaca
    45241 aagtcaacac cttgcccagc aaaagcaaga agatgatcaa acatttttgc caatacagaa
    45301 acaaccgctg taccaaaagt tgtacgtaat aatacgaaac caagaacaag ctgtaaaacg
    45361 aacatcacaa ttagtggttt caatttgatt gcttttttgt tactgctgat caaaaaagca
    45421 agccctaatg tgacaaataa accaataata cctaataaat aactcaaata atcactccta
    45481 ttccaaattc cgttttattt tacctgtttt gcaaataaaa tactaatctt tagtgaaatt
    45541 ttcattactt ttttagagtg aaacgcattc attttccatt agaaaataaa gcgaacaaaa
    45601 taaaaggttt aaaaagtgta acgtcttagt aaagtctata aaaaaacgaa actatcgatt
    45661 aagctaatag tttcgttttc aacatttata agtaaacttt ttctattggt aatatagatt
    45721 ttcggaaggg tagacaggtg cgcaggtaat atcgattcct agttttttca atgtttgttc
    45781 atcattttta ttgaccatca cagtagagtg ggcttgtgct ccctcaagtt cagctagttt
    45841 atcataagca acttgagcga ttggattcgt tactgcactg attgataaag cgatcaagac
    45901 ttcattgatg cttagtgcat ccaaagagga atgaagtgtc tttgatttca atccttgtat
    45961 tgtttcaaga actaatggtg acaataaagg aatgtcatct gcgatatggg ctaaatgttt
    46021 tactgcattt aagataactg ccgctgacgc atccatcaat gaacttgtac gcccggtaat
    46081 gatgacttca tctggtaatt cgattgcaat cacagcagaa ggatcttttg taggactttg
    46141 cgcttgaatc ttatctgcat agtcatgggc agggactacc acgtttcggt cttcaggacg
    46201 taaaccgact tcttccataa tcagtttgat tcgattaact gcttcttcgt cgatcaatcc
    46261 ttttttgaag tcgcattctg tttggaaata acgacggatg atttcttgtt tagaggcttc
    46321 ttggacaacg gtatcatcca cgatcccaaa tcctacacga ttcacaccca tgtcagtagg
    46381 agattgataa acagattctt taccagtaat tttttcaata atacgtttga ttacaggaaa
    46441 tgtctcaaca tcccgattat agttcacagc tactttattg taggcatcaa agtggaagga
    46501 atcgatcata ttgacatctt ttagatcgac tgtggctgct tcataagcaa tgttcagcgg
    46561 atgtttcaac ggaacattcc atactgggaa agtttcgaac ttggagtaac cagctgcatg
    46621 tcctttttgg ctttcatgat acagttggtt caagcaagta gcgagctttc cacttcctgg
    46681 gccaggagct gtcactacaa cgattggttt agttgtttca atatactcat tttttccaaa
    46741 accatcttct gagacgattt tttctacgtt gactggatag tcttcgattt ctgagtgttt
    46801 atagaccttg atgtttcgtc tttctagttt attgataaag actttcgtgg caggctgtcc
    46861 actatagcga gtgataacga cactgttgat taataagcca taaccacgta attcatcgat
    46921 taaccgcaag atatccatat cataagtaat cccataatca ccacggatct tattacgttc
    46981 aatatctcca gcatagacgc aaatcagtat ttcagcttga tctttcaatt tttgcagcaa
    47041 tttgattttc gcatcttctt caaatcctgg aagtacacgt ttcgcatgct tgtcgtcaac
    47101 taacttccca ccaaattcta aatataattt gtcatagtgg ttcactcgtt ccaaaatata
    47161 cgctgactgc tcttcaatat atttttctga atcaaaacca atttttttca ctaggttccc
    47221 tccttaagta ctaaagcatt ttttcatttt tttgaggatt cgtcaactgg aagagcgaaa
    47281 ttcctcaaaa aaacaaagaa aatggtttaa accaacttgt ctagatagtt attcattggt
    47341 atgatagttt ttgataagga aggtgctaca tgaaaaaaag acagaaaaag aaaaacgcat
    47401 ataaacacta tatccgttcg atttttaccg gttatgaaag aatgcttgaa gatcccgaat
    47461 tggaacaatt gacgtttact tatctaaacg aagaaacgca gctaactcgt gatgagcatc
    47521 agcgcattca tttcacaaca agagatctgc ctagtaaata aaaaataaga taaaggtcat
    47581 cggattcgat tctgtcctat cagatggaaa gactagttga tcgaaaaaaa ggctgtacca
    47641 aattttggtg cagcctgttt tatggagcat tgcccgctct tttttataac aattttttac
    47701 agaaaatggt actattgaag tattaataag tgaaaaacag atgagtaaga ggaggcaagt
    47761 agtatgaaag agccagatac aaatgcaaat tatgagccag acgaactaga ggagacgaac
    47821 gcggatcagg atacaggaca agaatcgtct tctttttacg atatcaaaga aaaagcaagc
    47881 atgaatcttt cccaattatt agaagactta caggcttttg aaaaagctgt ccgcgaagaa
    47941 gatatccctg agatctaccg tttatacaat ggccgattga atgaagaatt aaaaagaagc
    48001 tcaaatgaaa atcacgagat cgatcaattg ctgatgcgta agatccatga tcgtttttta
    48061 caggaatttc cttttatgga acaagtagaa aacatctcgc caacgatgag ctattacaaa
    48121 atcggcactt attaccatga tcggcccacg gtcggtattg atgcaagtct gccagaaatt
    48181 tttgttttgc ctaagatcga tgaagagtgg gagaaatatt cccaaccgga gtcaacggac
    48241 tatgacaaaa aaatcaatga gctagacgca aaggtgatca ctgctcagac agaaattgaa
    48301 cgtttagatg aacaaataaa agaaatcaat cgacaaatga ctgatttgaa cgacaataaa
    48361 ggattcttga atcgtaaaaa aatcgaggaa gaaattcaag aattagagaa gaagaagcaa
    48421 gtactatcta atgaaaaatt aggctggctt ccttatattg agacgcctga aacaatccag
    48481 cagcaaaaag aagcattgaa gcaagaagca agagcagacc agcttcgtgc agccatcgta
    48541 gaaaaagaac aaaggcaaat cagacgctat ttcggtagca aagagggatt cggtcaagca
    48601 atccatgaat tcttgatgaa ttatcttggc aatgagaatc ctaataatca atctaatgaa
    48661 ggaggttccg agtatgaatg aacacgatga agcagaatgg acaaaaaatg aagaacaaac
    48721 agaagatcat gaagaaacga acacttcatt ggaagaacaa acaagtaatc ctctggatga
    48781 cacagaagaa atccctatgc tagaggaaac aaaaatacat gaaaaagaaa aagtaactcc
    48841 gacgaatacg aatactgcag aaaacaccca tgcttacgaa ataactgaaa atcccgggta
    48901 tttagaagga aaagagcaag atcatcaaga tatcgtcagt ttgattgaaa cagcggaagc
    48961 agaattagcg gatttacgta tgagaaaaga tttgatcgaa tcagattttc cacaattatt
    49021 agaatcatat gatcgtttta tattgaatga agaaccaatc tccacgatcg gaaagacaag
    49081 cctgatcgaa tacttcacgt atgaattact gaaaccgttg aatgatattg gtctggaacg
    49141 gtcaaattca gcttatgaca catggaaaac aagacatttt tcgatcaatt atacgttgaa
    49201 tggacaaaac gaattagtct tttcctttgt attaccgacc atcgatcaac gataccaagt
    49261 ggaatcgatg agtctcctaa acgtatcacc agagacaatg gaaatcaatg tccaagatga
    49321 ccgggtgctt tctttgatcc gacactggtc agtggatcga gtattctctg cagggcagat
    49381 cacgatattc aatcacaaat tgaatcaatt attagcccat gcaagaacac ttggttttac
    49441 cgttaatcag acattactag acaatacgaa gccacttcgt ttgaatcttc agagcgaatt
    49501 tgaattgacg aatgaagtat tggatgatat ttttatcgtg gcaatgaaaa atccatccta
    49561 cgatatggaa aagatcaacg aaactactta tcaagtacga ttagataaag gacagtcttt
    49621 aacgataaca acaaacgaaa aagatcaaac agtcttgtcg atctcatctg gagactaccc
    49681 acgatctgtc attgatttct ttatcaatta cgaattcttg gtcccattaa tggtgagaaa
    49741 gtcctgattg gataagttgg attaccagat gtcattcaga taattctgtt ccaaaggact
    49801 tgcggcagtt atccagaata attgtcacaa gtcttttctg tatgtactaa atggacgtga
    49861 ataacttgct gatgaaggag ggatttcatt aggaatgtcc acaaataaaa tagcacagct
    49921 tagcttattg tctgctgctt gtgtagcagg taggatcgct tttcaattta ttccgaattt
    49981 ccagccaatg actgctattt ttttatttat agtactttac ttaagcttaa aagatgcgtt
    50041 aattgtcatg agtctttcta ttgcgatcag cagtttttat tttggtgttg gaccatgggt
    50101 catcggccaa tggatcagct atactgttgt tctgagtttg ttttcaatcg tctgcttgag
    50161 aaaaacagtg caaaggaatc tatggttaaa gggagtctgt ttttttctgg cgggcatggt
    50221 ttacggaata ggtatgaccg tttttgatac gctgttatat cgtttgcctc aaccttggat
    50281 ctattacctt caaggggtgt cgtttgattt gatgcacagt ataggcaatg ttgtgttctt
    50341 tcttgttttt ctgccagtcg tcaaacgttt ttacaatcat tcaggaggaa aaaatgaaaa
    50401 aaataattta tagtttgctt gcaatgagtg tcagtatact cattttgaca agttgcagta
    50461 caacctatac acaatcggct tccaaccaaa aggaggaagt aaaaacatcg caaaaagcaa
    50521 caatcacgtt acaagaaaat ggtcaaaact tttcggaaaa agaagtggtc ttcaaaaaag
    50581 gcgatgattt actgagtatt ttgaaacgaa attttgaagt caaagaagat aacggcttca
    50641 tcacttcgct tgaagggcac tcacaggatg agaaaaatca actctattgg atgtttactg
    50701 tggacgggaa aagtgctact actggtgcca aagagatcaa attgaatgat ggacaatcgg
    50761 tcattttcaa tttgtctaaa ctataagttt cataaaaaac tgttaaaggg cgaatattgc
    50821 cataattcag tgagttcagt tgatgaaaga aggaaaataa catggaaaat tttaaaaaaa
    50881 gcggaatgtt ttccgtaatt gctgctctag gggctaatat tctagttgct atcagcaaat
    50941 ttgtcggcta tgcaatcagt ggcagtgccg ccatgctaaa cgaaagcatc cacagtatcg
    51001 ttgattgcag caatcagatt ttgctgttat tcggtgataa acaagccaat aaaggacaga
    51061 gcgagttgca tcaatttggt gaaggacgag caaaatactt tttcagtaca atcgtcgcaa
    51121 tgatgctttt cttcggcggt ggggcacttg gggtgatgga agcttttgaa aagttgacgc
    51181 atccatccca tgaagtgggt aatccgatca ttgttatagc aatactgtta tttggtttgg
    51241 taatcgaagg aagttcgtta cgcgtggcaa tgaaagaaat caaagaattg aatactgaaa
    51301 aactttccac gatgcgcttt ttgcgcgaaa gtcgacacag tgaaatactg attattttta
    51361 cggaagactt gtgtgctgta atcggtttgg tcttggcttt agcaggaaca atattgactt
    51421 atgcaaccgg gattgctgca tttgatgcga tcagcggatt gctgatcggt ttcttgctga
    51481 tgggagccgc cattttccta gcaaaagaat tttatagtct gatcatcggt gaaagcgtga
    51541 cagcaagcga tttatcaaaa atcaagttag cttttgaacg tcctgagatc agtcggctga
    51601 tcgatataaa aacagttcat ttaagtccaa cggagatttt agttgcagca aaagcagata
    51661 tcgttggtaa caaagagaat gaagcttact cggtcatcaa tgatattgaa aaaaatatgc
    51721 gtcagtctct tcctgacaag aagatgtata tatacattga aacagataaa tatgacccaa
    51781 attattctcg taaataaaac gtttattcat gaattgaagc aaaaatgaga aaaatataat
    51841 cttgttttta aataaaacgg ctacaattta gcgaatttat gttacaatga gtccgctgtt
    51901 tatttgttgt aattaaagga gatattttaa tgaacaaaaa gaaaagtgcg ccatggcttc
    51961 aagctttgca tgcagtgatg ccactttgta taagctatat accagtagga ttagcttgcg
    52021 gagtattgtt gcaaaaagtt ggatttgatc ctctcttatc aggactgtta tcgattctgg
    52081 tcttttccgg tggcgcgcag tttttagttg catctatgct tacgacgcaa gcatcatttg
    52141 caacgacatt attgatggtt ttctttttag aattacgcta tacattatta ggagcaagtc
    52201 ttgccggttt tatgaaaaaa gaaaagcgga cgtttttggc tgtcttttct caatcactga
    52261 atgatgaaaa ctatgcggtg aactacttaa agttttcaac tgacccttcc tggaataaaa
    52321 gaaaggcttt atatgtcaat tggttctcaa tgactgcatg ggccggtagc aatatgatcg
    52381 gtaatttttt cggctcggtg attcgagtag atgctgacct tgtccatttt gctttgacgg
    52441 ccatgttcat tttcatgttt atcatgcaga tgaaaagcgc actactgatc tttacaggtt
    52501 tattctcagg cgtattaggg gtcgtattca tggtattatt ccaaaatact ttcggattga
    52561 ttgcagcaac agtcattgct tcatttgccg gatttatgct ggagaaggca ttcaaaaaag
    52621 aaaaagctaa gaaactaaga gaccgcggta aagatgtcaa tgagccgctc ttcaacttcc
    52681 cggaacagga ggccaatcac catgactaaa ctatatttgc tacttttagt tgcttgtctg
    52741 ttcatcgtgg catatatccc gcgcttgttc ccgatgcttt attttactca tcgaaaagtg
    52801 ccatcctggt tcagtgactg gatgaaatat gtaccggtgg cattatttgc ggcgttagct
    52861 tttaaagatg ttttcattac tcatgaacat ctggatattg cttggaatat caaaatagct
    52921 gcaatgattc tagtagcagg cgtggcttac aaaacacgtt cgatggcgtt gtcagttttg
    52981 acaggacttg cgtctgtctt tttgctgtct atgctttaaa ctgttttatt ttattaaaaa
    53041 cagagattgc gacataagtt gttcaaactg atctatccga actgcatttg caacagtcaa
    53101 ttctggatgc aagtttattc gtagggataa aaaagattag tgttggctta tgatgcaagc
    53161 tcttttttat tttaggaatt agagagggat ttgtccttct atgatttttt gctataatag
    53221 ggaagatgtt tattttatga aagaatagtt gagggaattt aaatgaataa aaaattgatg
    53281 caaggaacat tttggctaac ctttgctaat cttctatgta aagttttagg ggtagtctat
    53341 ttgattccct ggctaagtat gatggggaac aatcaagatg gtatgcttgc tacgacattg
    53401 tataatgttg gctatcttcc ttatggattg ttcctgatgc ttggtacagt cggatttcca
    53461 aatgcgattg caaaaaaagt agctgtagcc acaaaagaag gagataacgc tgcttgtcgg
    53521 ctgattttta gaagtacgat caatatcatg tttgtcatcg gtatcgcctc tgctgcactg
    53581 atgtatttat ttgctccttt actagccgga atcagtccga ttgctaacgt taataatggg
    53641 atactagcta tcagaagttt atgcccatct ttgattgcta tcccaattct tagtgcaatg
    53701 agaggatatt tccaaggaaa aaatgatctg cggccttatg gaacttcttt gattatcgaa
    53761 caggctgtgc gagtgatcgt tatcttggct ggtacttatt acttgcgcgt attgactgac
    53821 ggaaccatat tagaagctgt attgatcagt acagtcgctt ctttcttcgg tgggttagct
    53881 gcgattatcc atatgtatat cgtcggacgc agaaaagact tcttcgaact taaagatttt
    53941 ttgatttcca gccgttattt cgaaagagaa aaccgatctg cctctgtctc tatcattcgg
    54001 gaaacactac cgtttatttt tgtcggctca gtcattacta tcgttcaaat gatcgaccaa
    54061 gtaacgatga aaccactgct tcactttttc cgtccagaga tcgcagatca gcaactggaa
    54121 ttcttattca gccgtgcttc tgtcaatcca aataaattga cattgatcct gatctccatg
    54181 gtaggaacag tagctataag cagcttgcca atattaagca cgatgaagaa aaaagatcgg
    54241 atacaaatcg aaaaaacagt aggagatagt ttttcgattg cgttattgat tcttcttcct
    54301 tcattgactg gaatgtcttt attagctgga ccactataca cattgttttt tggctatgat
    54361 cctgaaagtg tcggatattt ccaaatggct ttgctagctt ctttgttttt ctctttattc
    54421 acgatcctat ctacgatgct tcaatctttg aatcaccatc gttttgcgat ccgattaact
    54481 gtagaagcaa ttatcctgaa agtcatcttc caagtaatcg gattaggatt agttggcgga
    54541 tacgggatga gcttatcaaa tggcttagca ttcggtattg tctttgttcg cggctatcac
    54601 tttatgtgca aagaataccg aatagcacct ttagcaaaga tcagcaattt tttcttaaaa
    54661 actttccgct ccaccctgat tatgcttgca gtatgttttg ccgttttctt tttgctgaat
    54721 cagcgattgt caatggaatc aaaaagctat gcaatgattt attgtgtggc tgtaggaagt
    54781 attggcggct tagtattttt attttctcaa ttcggaaaaa atgggtttcg aatgttgaaa
    54841 aatgttaaac atcatagcag aagcaaagaa taggttaact aatcgttttt tgctgtcact
    54901 tgaaaaacgg caactcgtat gattacgctt gtcgtttttc tattttccaa ttttagtttc
    54961 atatctaatc ttacttatga tatagttata aggtctggaa aaattctttg aaacgaggaa
    55021 attaaacaca tgaaacttgc agtagttaca gatagttcgg catatctgcc ggaacgtatc
    55081 aagaaccatc ccgacttatt cgttattcca atccctttga ttatggatgg gaaaatttat
    55141 aacgaaggaa tcgatatcga agcggatgaa tattatggac ttttaaaaaa cagtaaagat
    55201 tttcctacta cttcacaacc tgtcgtaggg gaagttttgg gtttatatga ggaattgaaa
    55261 gagaaagggt atgacacgat tttaagtatc catctttctt cagggatttc tggctttgtc
    55321 aacacattat ttgcgatgaa agatgacatc agcggagtaa cggtcattcc ttatgactct
    55381 aagatcacta gtatgccgat gggacacatg gtagaagctg ccttagactt aaatgatgaa
    55441 ggtcagtccc ttgatgaaat acttgcacat atcgatcgaa tccgagataa tacctatgct
    55501 tatctgattg tagatgattt gaacaattta gtacgaggag gacgtttgac aaatggtgca
    55561 gcgctgattg gcagcttatt aaaaatcaaa ccgatcctga cattttctga agggaaaatt
    55621 gttttattcg aaaaaatccg ttcaagtaaa aaagcatttg ctcgagctga agaaattatt
    55681 ggtagaagaa ataacgaaat acaaccgcca gtcaaacttt acgtgataca tgcaaataat
    55741 ttagaagtag cgattgcgga gaaagagaaa ttgcagaaaa aatatccaaa tgcagaaatc
    55801 gaaattggtc atttcggacc agtcattggt acacatttag gtgaaaaagc catcggcatt
    55861 gccatttcag cgcaataatt ccttttattc aaaaaacata ccaaaaat
//
LOCUS       DACECY010000011        55756 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.011, whole genome shotgun sequence.
ACCESSION   DACECY010000011 DACECY010000000
VERSION     DACECY010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.011"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..547
                     /locus_tag="IWO38_000688"
     CDS             <1..547
                     /locus_tag="IWO38_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HAQ0312800.1"
                     /translation="KIYTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSI
                     CSIPYNNIEEIIEKDAISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIV
                     PQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKAD
                     YVTVSNDKDGVSQAFERILTI"
     gene            828..2048
                     /locus_tag="IWO38_000689"
     CDS             828..2048
                     /locus_tag="IWO38_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ0312801.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2045..4765
                     /locus_tag="IWO38_000690"
     CDS             2045..4765
                     /locus_tag="IWO38_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0312802.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4749..5693
                     /locus_tag="IWO38_000691"
     CDS             4749..5693
                     /locus_tag="IWO38_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0312803.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(5762..6799)
                     /locus_tag="IWO38_000692"
     CDS             complement(5762..6799)
                     /locus_tag="IWO38_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0312804.1"
                     /translation="MTNYRSANFNEKKSIILAATSALAVLTLAACSGDTNKDIATMKG
                     GTITVSDFYDEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDT
                     FESQLEAAGYTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKL
                     SSEDEAKDVKKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFK
                     LKDGEISDVITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAF
                     TTKVIGEELKDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKE
                     SSTEETTDSSK"
     gene            complement(6951..7295)
                     /locus_tag="IWO38_000693"
     CDS             complement(6951..7295)
                     /locus_tag="IWO38_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0312805.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7288..7722)
                     /locus_tag="IWO38_000694"
     CDS             complement(7288..7722)
                     /locus_tag="IWO38_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ0312806.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            7860..8597
                     /locus_tag="IWO38_000695"
     CDS             7860..8597
                     /locus_tag="IWO38_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0312807.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            8590..9801
                     /locus_tag="IWO38_000696"
     CDS             8590..9801
                     /locus_tag="IWO38_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0312808.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10008..10787
                     /locus_tag="IWO38_000697"
     CDS             10008..10787
                     /locus_tag="IWO38_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ0312809.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            10857..11519
                     /gene="trmB"
                     /locus_tag="IWO38_000698"
     CDS             10857..11519
                     /gene="trmB"
                     /locus_tag="IWO38_000698"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ0312810.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(11617..13371)
                     /gene="ade"
                     /locus_tag="IWO38_000699"
     CDS             complement(11617..13371)
                     /gene="ade"
                     /locus_tag="IWO38_000699"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ0312811.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13503..14432
                     /gene="cpaF"
                     /locus_tag="IWO38_000700"
     CDS             13503..14432
                     /gene="cpaF"
                     /locus_tag="IWO38_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ0312812.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14389..15438
                     /locus_tag="IWO38_000701"
     CDS             14389..15438
                     /locus_tag="IWO38_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ0312813.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15435..15713
                     /locus_tag="IWO38_000702"
     CDS             15435..15713
                     /locus_tag="IWO38_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ0312814.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            15688..16158
                     /locus_tag="IWO38_000703"
     CDS             15688..16158
                     /locus_tag="IWO38_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ0312815.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16175..16477
                     /locus_tag="IWO38_000704"
     CDS             16175..16477
                     /locus_tag="IWO38_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312816.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            16494..16865
                     /locus_tag="IWO38_000705"
     CDS             16494..16865
                     /locus_tag="IWO38_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ0312817.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            16837..17196
                     /locus_tag="IWO38_000706"
     CDS             16837..17196
                     /locus_tag="IWO38_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312818.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17368..18375
                     /locus_tag="IWO38_000707"
     CDS             17368..18375
                     /locus_tag="IWO38_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0312819.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18400..19584
                     /locus_tag="IWO38_000708"
     CDS             18400..19584
                     /locus_tag="IWO38_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ0312820.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20021..21604)
                     /locus_tag="IWO38_000709"
     CDS             complement(20021..21604)
                     /locus_tag="IWO38_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0312821.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22182..22325)
                     /locus_tag="IWO38_000710"
     CDS             complement(22182..22325)
                     /locus_tag="IWO38_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ0312822.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(22548..23027)
                     /locus_tag="IWO38_000711"
     CDS             complement(22548..23027)
                     /locus_tag="IWO38_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0312823.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23100..23384)
                     /locus_tag="IWO38_000712"
     CDS             complement(23100..23384)
                     /locus_tag="IWO38_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312824.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23462..23656)
                     /gene="ssrS"
                     /locus_tag="IWO38_000713"
     ncRNA           complement(23462..23656)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IWO38_000713"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23728..25014)
                     /locus_tag="IWO38_000714"
     CDS             complement(23728..25014)
                     /locus_tag="IWO38_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ0312825.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25152..25823
                     /locus_tag="IWO38_000715"
     CDS             25152..25823
                     /locus_tag="IWO38_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ0312826.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            25789..26277
                     /locus_tag="IWO38_000716"
     CDS             25789..26277
                     /locus_tag="IWO38_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ0312827.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26290..27237
                     /gene="prmA"
                     /locus_tag="IWO38_000717"
     CDS             26290..27237
                     /gene="prmA"
                     /locus_tag="IWO38_000717"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ0312828.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27238..28002
                     /locus_tag="IWO38_000718"
     CDS             27238..28002
                     /locus_tag="IWO38_000718"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ0312829.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28235..30448
                     /locus_tag="IWO38_000719"
     CDS             28235..30448
                     /locus_tag="IWO38_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ0312830.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30459..30905
                     /locus_tag="IWO38_000720"
     CDS             30459..30905
                     /locus_tag="IWO38_000720"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ0312831.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31076..32335
                     /locus_tag="IWO38_000721"
     CDS             31076..32335
                     /locus_tag="IWO38_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ0312832.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32424..33371)
                     /locus_tag="IWO38_000722"
     CDS             complement(32424..33371)
                     /locus_tag="IWO38_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ0312833.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            33765..35057
                     /locus_tag="IWO38_000723"
     CDS             33765..35057
                     /locus_tag="IWO38_000723"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ0312834.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35072..36841
                     /gene="aspS"
                     /locus_tag="IWO38_000724"
     CDS             35072..36841
                     /gene="aspS"
                     /locus_tag="IWO38_000724"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ0312835.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            36950..37825
                     /locus_tag="IWO38_000725"
     CDS             36950..37825
                     /locus_tag="IWO38_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0312836.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38091..39974
                     /locus_tag="IWO38_000726"
     CDS             38091..39974
                     /locus_tag="IWO38_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ0312837.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40049..40333)
                     /locus_tag="IWO38_000727"
     CDS             complement(40049..40333)
                     /locus_tag="IWO38_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0312838.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40323..41057)
                     /locus_tag="IWO38_000728"
     CDS             complement(40323..41057)
                     /locus_tag="IWO38_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ0312839.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41186..41809
                     /locus_tag="IWO38_000729"
     CDS             41186..41809
                     /locus_tag="IWO38_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ0312840.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42001..43122
                     /locus_tag="IWO38_000730"
     CDS             42001..43122
                     /locus_tag="IWO38_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ0312841.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43129..46257
                     /locus_tag="IWO38_000731"
     CDS             43129..46257
                     /locus_tag="IWO38_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ0312842.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46276..49470
                     /locus_tag="IWO38_000732"
     CDS             46276..49470
                     /locus_tag="IWO38_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0312843.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILPLYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(49616..50323)
                     /locus_tag="IWO38_000733"
     CDS             complement(49616..50323)
                     /locus_tag="IWO38_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0312844.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51088..51486
                     /gene="spxA"
                     /locus_tag="IWO38_000734"
     CDS             51088..51486
                     /gene="spxA"
                     /locus_tag="IWO38_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ0312845.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            51818..52471
                     /locus_tag="IWO38_000735"
     CDS             51818..52471
                     /locus_tag="IWO38_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ0312846.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            52697..53695
                     /locus_tag="IWO38_000736"
     CDS             52697..53695
                     /locus_tag="IWO38_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ0312847.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            53871..55679
                     /gene="pepF"
                     /locus_tag="IWO38_000737"
     CDS             53871..55679
                     /gene="pepF"
                     /locus_tag="IWO38_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ0312848.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        18324 a         9659 c        11581 g        16192 t
ORIGIN      
        1 caaaatatat acaaatctcg gaagttatga atctcagaat tataaaaata aaatgaaaaa
       61 aatttttcac gaggtaggga gtgaattttc tatcaaagag ctaaactatg agcttgagta
      121 tgaaaaatct atttgttcaa tcccttataa taatattgag gaaattatag aaaaggatgc
      181 tatcagcatt tacaaatttt ttatttttat tcctaatcgt caattgaaag ctaatatatt
      241 taaccatttg aaaaagatgc taaatgtgtc tgttactgaa tcggcacctg taaatattga
      301 aatcgtacct caaaatgtat ctaaaggtgt ggtgtttgat cgtttggaat caatctacaa
      361 tttaaagaaa cctttgagaa tcgcgatcgg agatagttta aatgacttat ctatgtttga
      421 aagtgcagat attagttttg caatgggaaa tagtcatcaa gaaatcatga aaaaagctga
      481 ttatgtcact gtttcaaatg ataaagacgg agtatctcaa gcatttgaaa gaattcttac
      541 catataaact aattaaggta gtatagaaca tagaattcta aatacggaaa aatggagcat
      601 aaattccagt acaaatagta cttcttcagt gtcaaacgaa gcgaatataa catagatatt
      661 taggattgtg acaaaaatca tgtcacaatc tttttttcga ataaacttat tttttgaagt
      721 cggccattta tcgtaatttc tttgcataat acgaacaaat tttcggttat tgatgaaaaa
      781 aaacattgaa aatggtatga taaaagaaaa aaaggaggaa atttttaatg gtgaaattta
      841 tccatgcggc agatttacat atggatcggt catttgaagg actgacaact cttgataaaa
      901 ctgtacagga aaaactattg aagacgaatc tgaccgtttt atcaaatatc attgaacagg
      961 caataataaa taacgttgat tttgttttac ttgcaggaga taattttcat caaaatcgtc
     1021 cttcattaaa aatacagaaa catttttcag aacagatgaa acggttagaa aagaaccata
     1081 tacctgtatt tataatattc ggaaatcatg atttctatca aaaagagcgg tattggttct
     1141 cctttcctaa aaatgtccat ttatttacat ccgaaacggt agagacaaaa aaaataacga
     1201 taaaaagtgg agaaactgtc tctctcagtg gatttagtta tcgtcaaccg tggattcaaa
     1261 aagacaaagt gatggaattt ccttctagag aactcacaga ttaccatatt gggttgtacc
     1321 acggggaacc tggcgtctct caaaaaggaa actatgctcc tttccaaccc tcaaagatgc
     1381 aagaaaaggg atacgattat tgggctttag ggcatatcca tgtgccaacg gtcttaaatg
     1441 aaaagcaaac aattgtttat ccaggtgcgc ctcaaggtca tactaaaaag gaacaagctt
     1501 ctacatctat tctactagtt gaattaagta aaggaagttg ccaaatacat ccgattaaag
     1561 tagcagaggt ctattggaag acaaaggaaa tatcattgag gaaagcaaga acgacaaaag
     1621 aggtcattgt tcacattcgt cagcagcttt caaaagtaga ggatggattt agtttaattg
     1681 agataaaatt aaaagattat gaccatctca atacagatgt tttggaacgg atacaatcag
     1741 gagagctatt agactatttg ttagaggaat tctcagatag gataaatgat ttctttattt
     1801 ggcgtatttc tctcatagag aatacttttt atacgaagac cccacttgct gcttctgcta
     1861 aattgatgga acagttattt caatactatc agacacctca agatttccaa caaattttaa
     1921 atgaagtata tagtcatcaa gaagcggcta gaatattgag cgaactgccg gaatatcaag
     1981 aagaaacact tgaaaaagcc aagcagctgc ttaatcaaga ttttttattt gaggaggatc
     2041 aggaatgaag attttaagga tcgagattgc tgcttttgga aaatggcgtc aaaaaagttt
     2101 tgacttttat tcaggcaatc agctgatcta tgggggaaac gaagcaggaa aatcaacgat
     2161 ttatcaattt attcaagcaa tattatttgg tttcccttca aaaggccgta aaaaaaagga
     2221 ttatacacca aaagacggct cagcctatgg cggcaaaata tggcttaaac atcctgtata
     2281 tggtgaattt gctgtcgagc gctacaagca gcaaaatcgc ggaaaatcga aggtgtggct
     2341 gggggatcaa gtgggaagtg atgagttact agaaaaactg atcgcacctt tgacgaaaga
     2401 gttattccag caggtattta ctttccagca agagcagttg atggaactag atcatttgag
     2461 agaaaaagag ctacatgatg cgctgatttc tttaggcatc acgggcagtt ctcaagtatt
     2521 ccaaaagcgg caagaatatt atcagaaagc acaacaatta ttcaagaaaa aaggccaaaa
     2581 attactaatc aatcaaaaat taaatgaatg gaaagagctg caagaaaaaa ttcatcaaaa
     2641 acaagaacag gaagcttatt ttggcaagtt gatccaacaa gaacaggaat acgagtcgca
     2701 acagcagcag ttattggcac aattaaaaga ggcgaacaaa caattttcat tagccagaca
     2761 gcaagaactt aatttttctt tatatgaaga atggcaatct ctcaaaaaaa acagcactac
     2821 aggtcttcct gatgagcaac ttcttttaga tctggagtct ttctccaaac ggtatcagca
     2881 aacaaatgag cgtttggaag aattagaagc aacactggaa aagcatagcg ggatggatca
     2941 gcagtcagcc agatattatt tttatttaga gcaggagcag gaactaaaag atctccagca
     3001 acttcaattt acagcggaac aattaaacga agaatggcaa agattacaaa ttgagactag
     3061 agaaacaaat tcagaattga agattttgga acaaaggtgg cattggcaga aaaatccgtt
     3121 accggaagaa tttttatatg aagaagaatg gaagcatctt ctggctgaag aaagaaggat
     3181 tgctgatcta gaaagccaga tttctgtgcg tctccaaata gctaaagaac agcaagagat
     3241 gattgaagca gaagtgaatg acttggaaga gacctatcca gcattattac aaaatcagtc
     3301 agagatagca ccagtctctt catctaaacc gtatatgctg atggctggcg gagcaataat
     3361 cattgcaggt atatttgtac caatgccctt gaaagtgatc cttttactaa ttggtgcagg
     3421 catgattggc tatttcttct acaaaaggca accaaaaatt aaaactaaaa atgatttctc
     3481 tgagataaaa ggaatgtggc aagaaaaact aggacaactt gatctagcct tagctcaatt
     3541 agctgaagca gaagaaaaac aaaagagaac tggagaagaa aaacagattc ttcagcaaga
     3601 agtatatcgt tttgcggatc atcggcactt aggaaagatg gatacgctca aactcatgaa
     3661 agaatatgga caggaagtca tagattatca gaaattatat ggcgtatttg agcggaaaca
     3721 taaaagagaa caggaaatac aagaaaaact acgacaaatc gaccaaaaat ttgagtttgt
     3781 taaagaatgg ttaccgatac aagataagac aatttcagaa aaaatggtat tgctttcccg
     3841 ttttatacaa gagatggaac agatgaaatt tgccagaagc tatcagcaaa atactttatt
     3901 gaagcaagaa atcaatcaat tgaaaaagaa acaaaaagta tggatacaag aatacagcga
     3961 acaattgact caagcaggct tatcttatcc ttcggaagta cctgtctttt tacaaaaagc
     4021 caaacaagcc gaacagcaga tgaaaagata caatgaatta tcggaaatgc ttactccact
     4081 atttcctgaa gagctgacaa aagagaaatt atctgtaaga ctgcgccaac tgaaagaaaa
     4141 gcaatcccaa ctccagcaag aagaaaaaga agcaagtgaa aatcggcaac gactccaact
     4201 tcaagtagaa gagttgcaaa gagacggcac attggacgaa ctttatcaac aagctagccg
     4261 gcaaaaagca aaattacagg aattactgga ggaatgggga gcacttgaaa tcgccgggac
     4321 attcttaggt gatctttcaa cagagatgag tgaaagacaa ttaccccagt tgttaagagc
     4381 agcaggtgat tatttaggta ttttaacaaa aggaaattat cagaagatat tactgaaaaa
     4441 tgatacactg actttaacag acggatatac agaatttccc atctatgagt tatctacagg
     4501 tacgaaagat cagctgatta tggcgatgag atttgcttac ctctcattag aagctaaccg
     4561 ttcgatatgc ccgatcatca ttgatgatgg ctggcttcat tatgatcatc agcgaaagta
     4621 tcaattagct gaacttttag ctaaatttgg agaaaagtat caaataattt gtttttcttc
     4681 tgataaagaa atggtaagct attaccatga gatgaaccaa atcgtaaaac aattagaagg
     4741 agcaaataat gaaaaaaatt cgtgatttag taatcgatga gcagtttgaa caatttgtcc
     4801 tgatcaaaag tgcagatgta agaatcgcta aaaacggaaa aaaatttatt gcttttactt
     4861 tccaagatac ttcaggtact atcgatggta aatactggga tgcttcggag gaggaaatca
     4921 ataaatttac agccggcaca gtcgtttttc taaacggtaa aagagaggtt tatcaaggaa
     4981 accctcaagt aaagattctt catatgcgct taacaagggt agatgagcca ggagagccta
     5041 gtttatacat ggaaagagcc cctttgaaga aagaagaaat ggaagaagaa atcaatcaat
     5101 tgttgtttga gatcaccaat gcgcattgga atcgtattgt tcgctatcta cttaataaat
     5161 atcaaaaaca attttttgag tttccggcag caaaaagaaa ccatcatgca tttgccagtg
     5221 gattagctta tcatactttg acaatgcttc acttagggaa atcaatcgcc gatgaatatc
     5281 ctgagctgaa tcgagcattg ttgtatgctg gtatcatttt gcatgattta ggaaaggtga
     5341 ttgaactttc cggtcctatg tcgacagaat atacgcttgc aggaaatttg atcggtcatc
     5401 tggtattgat tgacgaagaa ataactaaag cctgtcttgc gttgaaaatc agcgaagctg
     5461 acgaagatgt tctcgtatta cgtcatatgg ttttagcaca ccatggctta ttagaatatg
     5521 gctcacctgt acgtccaaga ataatggaag cagaaatatt acatcaaatc gacaatatcg
     5581 atgcttcgat gcagatgatg ttgacttcga ttcgtcaaac agaaccagga aaatacaccg
     5641 atcggatttt cggtatggac aaccgaagtt tctatgtacc aaaagattta tagcaaaaat
     5701 ttattttttt cattcaaaac aaaaaaagcg agacataagt cttcgctttt tttgttttct
     5761 tttattttga tgaatcagtt gtttcttcag ttgaagattc ttttgttgaa tcagttgatt
     5821 ttgtgtcaga actctttgtt gaagctgtag tttcacttga gtctttcgta cttgatgaag
     5881 ttgttgtgaa tgcactcaac acattttcaa aggcatcatc tttgattttt acatttgcat
     5941 cttttaattc ttcaccgatt actttagttg tgaatgcatt gtcacttagt ttagtttcag
     6001 tagcgatatc ttttagttga tctttgtatt tatccatgtc attgccttta ttttggtttt
     6061 tcaccatttt gacaacgtag tattctgtcg cataagaagt aggatttgtt gtagtaataa
     6121 catcggagat ctcaccgtct ttcaatttga aagctgcttc ttttacttct gcaggaatag
     6181 ttgttgtcgt tgagtcgaat ttaactttac cgccatcttc ttttgtctct gtatcagttg
     6241 atttgtcttt ggctaattta gagaaatcat ctccatcatc cgctgatttt ttcacatctt
     6301 ttgcttcatc ttcacttgat aatttgatga tttgtgcttc tacttctgga tggaaagatt
     6361 tccaagcagt tttcaaatcg tcatctgtaa tatctacgtg agctttcaag ccagcttcaa
     6421 aagctaaatt gttacgaata tattctttat aagtatcttt cgtatatcca gcagcttcta
     6481 attgtgattc aaaagtatca cctaatgatt tcgcttgttt gtcgtattct gcatccactt
     6541 gcttatcggt tactttatcg ccatatttgt tattgaatac tttataaatg atcatccgtt
     6601 gtactaagga ttggttcgat gattcaagtt ttgcttcatc atagaaatca gatacagtga
     6661 tcgttccgcc tttcatcgtt gcgatatctt tattagtatc gcctgaacaa gctgctagag
     6721 ttaaaacagc caacgcacta gtagctgcca aaataattga ttttttttca ttaaaatttg
     6781 cactcctgta attagttatt atttttatat attaactata ccatatccca tttcctttaa
     6841 aaacgttgtc gtggtagttt caaacaaaat tcaaaatttc atcacattcc taggaaatac
     6901 gcaaactttt ctcacttata tagttaaatc tgttgagaaa ataatcatta ttatatttgt
     6961 tcaggatact ctggcagatc agcttttagt ttttctactt gttcattgat ttgtgtgata
     7021 cgtggttcgg cttggaattt aaaatcttcg atatcttttt tgattgcttc ttggaaagca
     7081 ggaatcacag attcagctgt ttcttttgtt tcgatcaatg cgcttttaaa atgattcagg
     7141 ctgttattta aatcatttgt cagagttgtt gcttcttcca attcttcgat cagtttcttt
     7201 cttgtttcat tcccgctcct gggcgccaat aacagacctc cgacagtgcc agcagcagct
     7261 ccaaataaca gtcctttgaa aaagttttta atcatcgcag gacacctgct ttacgattgt
     7321 ttcttgaata gcttttagtt cttccgatcc ataactatct tgatgattga caaagtgaat
     7381 agaaaaatca tcctctttgc tgtagcgagg gactaaatgg atatgtgaat gaaaaacaga
     7441 ttgataggct agttcacggt tgttattcaa gatattaaga cctttcattt ctggaaaagc
     7501 tttttccaat gcttgagcaa tcttaggcac acgcgaaaaa acctctcctg caagttcggg
     7561 atcatattca aaaatatctg ctacatgacg tttaggcaca actaatgtgt gtcctttcgt
     7621 tgcttgagag atgtctaaaa aagcatagac cttgtcgtct tcatatattt tatagcttgg
     7681 gatttcttga ttgatgattt tgcaaaaaat acagttttcc atataaaaca tcctttctat
     7741 aaagaataaa tacttcagct taactttacc ataagttaaa gctattgtaa ggaaaaaaac
     7801 aaaataccgt cattttcttc gtggtataat aaataaaaat aaatggcgga ggaaaaagaa
     7861 tgagtttaat tatcgatcat gtcacagggg ggtacggaca tctccctgtg ttaaaagata
     7921 tcagtttcga tgtacagcct ggagagatag tagggttgat tggactgaac ggagcaggga
     7981 aaagtacgac catcaagcac attatcggtc tgctggaagc ttcaaaagga acgatagaaa
     8041 tcaatcatct atccttaaag aaaaatacgg aagaatatcg caaacaaatt gggtatattc
     8101 cagagactcc ttctttatat gaagaattga cattgagaga acatatcgaa gtcactgcga
     8161 tggcctacga tatacctatg gatatagcaa tgaaaagagc agaaaagttg ttggagactt
     8221 ttcggttatc taatcgtctt gactggttcc cagctaattt ttcaaaggga atgaaacaga
     8281 aagtgatgat cttatgcgcc tttttgatcg agccttcttt atatatcatc gacgaaccat
     8341 ttttaggctt ggatccacta gctatcaatg cattgcttga attgatggta gaaatgaaaa
     8401 aagaaggagc cggtattttg atgtctacgc atattttagc tactgcagaa aaatattgtg
     8461 atcgttttgt ggtcttacat aacggagaga tacgagcaga cggttctctt gagatgttga
     8521 gaaaagaatt tcagctgccg gattcttctt tggacgaaat ctatattgct ttaacaaagg
     8581 agcaggcaca tgagtagttt ttttcaaaaa cgtttagcaa gacatcaaaa gaaaatgatg
     8641 aaatatatgc gttatgtatt gaacgatcat tttgttttag tttgtctttt cttgattggc
     8701 ggtgtgggat tatattattc taattggttg aaaaccctgc agccaccgtt tgcaattgga
     8761 gggctcataa tctctgtatt ttggatgctc tgtttattcg tgggtaagat ggctactttt
     8821 gcagaggcag cagatattgt ttttttactt cctaaagaaa aagaaatgca aacctatttg
     8881 gaacgagctt ttcgctattc ttgcattttt ccgttgatct ttttgatatt ggcatgcgga
     8941 ttggccatgc cattagtggt ggttagtacg gagcaatcct tttcgatgtt tttcgtctat
     9001 ctgatcatat tatggagttt gaaattcagt catctgcaaa tcaagagagc agaattgttt
     9061 cgactagatc aacaagttat tcgttattgg aaattaggct ggtttttttc ttctctgatc
     9121 tttttgtttc tatcatttta ttggctaata tgggcaggaa tagcaggtgc attagtacag
     9181 gcaagtctat attactggtt tttgtggaag caacaagaaa aaggattgga ttgggagaaa
     9241 atggtgcatg tagaagaaca taggttgcat cagatctatc aatttatcaa tttattcaca
     9301 gatgttcctg aaattacggc aaagatcaaa agaagacgct atttggatcc tatattgact
     9361 catattaaaa gagaatcaaa aaacacttat ctttacttgt ttgcaagaag attggcaaga
     9421 ggttctgatt ttagtggtct ttatttccga ttagtaatta taggtgggtt attgattgcc
     9481 ggagtagtcg acttttattt tagtctgatt atcggtttct tatttttata tttgatcggt
     9541 ttccaactgt tacctttagc gaaccagttc cgctatatgg ttctcgttca gctttatcca
     9601 atccataaga atcagaagaa gcaggcgatt cagtatctac tcacatgggt attaattatc
     9661 gcgtcggtta tttttggttt gattgctgca gtcgtattag atgggcagca gcgatgggtt
     9721 ccgctagcgg cttatttgat tgttactatg ctctttgtta aactatatgt accaactcgt
     9781 ttgaagaaga tgtcagactg aaaacaaagc tgtgtagggt aaaaatgaga atgaaaaata
     9841 gagcgttgac agtaagaatg ctgttcgttc tatttttttt gagaaaagct ggttttttga
     9901 atggcaatta aatgaaaaag aacgtatatg tgcttgacgt gtagtgttca ggcgactaga
     9961 ataataagta ctgcaaaaca aagagtaaga ggacgggaga atgcatgatg gagtttcagt
    10021 tagaccaaga gtggcggtta cgtcccatca aaggcgacac aggaaaaaca tacataggca
    10081 tgcgtgatga ggacaaagtc tttattaaac gaaacacgac tccgatgttg gctgctcttt
    10141 caagggaagg aattacacca aaacttgttt ggacaaaacg aacggggaat ggcgatactt
    10201 tgacagcaca agagtggctg gaaggtcgct tgctgactcc taatgagatt ggtcaaagaa
    10261 acgacgtgat tgatgttttg taccatttac atcattcaaa ttctcttaaa accatgctga
    10321 cccgtatcgg tggtcgtgtc atgacgccgg agaagatgct gcaaaaatat gaaaaagaat
    10381 taccggaaga actccagaaa aatcaatttc tgcaaattgt ccatcgtttt cttcgtgagc
    10441 agatcccgtc aatagataag gaaaacttat cagtagtaca cggagatgta aaccatcgta
    10501 attggttagt atgtaaaaat tatttatatc tagtcgattg ggattcaatc atgtttgcag
    10561 accctgcggt ggatattgca acgattttag gaaattatgt gccactgtct agctggaatc
    10621 agtggctgat cagttatggc atacgcccga ctgacgaaat gcttgaaaaa tttcattggt
    10681 atgcggccat gaatttgctc caagaaatca ccagatattg caaacgtgaa gatcatcgac
    10741 ggatgaacga agaaatttta caattaaaac gaattttcag tggctgaggt ttggcccaaa
    10801 ctctgcggag atttggccag acctttttaa ttagaggaaa ggaatggaga taaataatgc
    10861 gtgtccgaaa tcgtccggga gcagcggaaa tgttagcggc tcacccgaat tttgtaatta
    10921 gtgatccaac attgtggaag ggaaaatgga atgaattgtt tgaaaacgat catccaatcc
    10981 acattgagat tggtatgggg aaaggccagt tcatcactgg aatggcaaaa gcccatcctg
    11041 aaatcaatta tattggagta gaaatgcagg tcagtgttgt ttcgattgca ctagacaagt
    11101 tgatcgaaca accgcttcct aacctgaaac ttctacatgt ggatggatca gcgttgacag
    11161 agtatttcgc tgacagcgaa gtagatcaaa tctatctgaa tttttccgat ccatggccaa
    11221 aaaaacgtca tgaaaaacgg cgattgacat ataagacttt tcttgctgta gatgaacaga
    11281 ttcttcgtcc aaatggagaa atccatttca aaacagataa tcaaggattg tttgagtatt
    11341 ctctggcaag tttttctcaa tatggcatga tcctcaaaca ggtatggtta gatttacatc
    11401 aaagtcaatt tgaaggcaat attatgacgg agtatgaaga aaaattttct tcaaaaggtc
    11461 aacgtattta tcgagtagaa gctcaatttc aagataagac caaaaagaaa agtctgtgaa
    11521 actaactttc tgctttcata aaaacattaa tatccataaa taaatcccag aaaatgaaca
    11581 ttacagatgt aatgtatttt ctgggattta tttgatttaa aatgtattta tttcatcggt
    11641 ttttccacaa ggtggaatca attggaagcg agaaaatgaa aataatcctt tgtcggtgat
    11701 cttcaattcg ggtatgacag ataatgttaa gaaagaaagt gtcaaaaaag gatcaaaggt
    11761 tttagctgct cctagttgat gtgctttagt tattaattca ttcaactgtt catagacgat
    11821 cgaaaaaggt tcctgtgtca tgattccgcc tataggaaga gaaacacaag ctatttcctg
    11881 ctgtttgttg acaacggtca tcccgccacc tttttgaatc aaaagattag cagcatacag
    11941 catatcctcg tcgtttgtcc caacgacaac tagattatgc gagtcatggg caatcgttgt
    12001 cgctatcgct ccttgtttca gctgaaatcc tttcactatg cctaatccaa tgtttcccgt
    12061 ttgatggtga cgctcgatca ccgcaatctt taattggtct ttttcgattg aaggaatgaa
    12121 tcgttcgcct tgtcggtcca ctttttctac caaatcttta gtcactagac tattcgggat
    12181 aatttcgatc acatgggctt gtgaagaaga aagaagcagc tctaacgaat gtggatttaa
    12241 tggttgagcg tttagttttg gcagacaaca cgtatatgaa tttgaatgat cagcaaataa
    12301 atggtcttgg agttgcccag attgaacaac taatgttccc ttatagaaaa cttcatgaat
    12361 tggcagcttg gtaagatcgt ctgttaagaa gaaatcagct tgatacccag gggcaatcgc
    12421 acctagtgtt ttcaaatcaa aacattctgc tgcattcaat gttgccatct gaatggcttg
    12481 taatggatcg attccaagtg caatagcttg acggacgata tgatcgattg atccttcttt
    12541 tactaaatca ttgatcagtt tatcatcggt gacaaagaag catcttcttg aattttcagg
    12601 tgtgatcgcc ggtagtagcg atgccagatc ttttgcaacg gtcccttctc gaaccattaa
    12661 atacatacca agatccaaac gttctttagc ttcttttgca gttgtagctt catgatccgt
    12721 ccgaatacct gctgcaggat aaacatttaa ctcttccatg ccgataccag ctgcatgccc
    12781 gtcaattttt ttcttatgct ggtgcatcaa ttggatcttt tctaggatgt ctgtttcatt
    12841 atttgctact gcttgatagt tcatgacttc agctaagcct aaaacttcgg gtttttgtaa
    12901 aaaaggggct aatttttttg catttaaatt tgcaccattg gtctcaaaag gggttacagg
    12961 tacacaagaa ggtagcataa aaaagatatt catgagtgtt tgtcttgcat cttctatcat
    13021 aaactcaaga cctgtactcc cagcaatgtt tcctatttca tggggatctg tcactacagt
    13081 agtaactcca tgaagcaagg atacttttga aaattcgctt ggtgataaaa gtgagctttc
    13141 gatatgcaca tggccatcga tgaatcctgg cactagatat tgcccattag cattatgtac
    13201 gctttttcct tcgtattcac cgattcctac gatcattcct tcgcagattg ctacatcttt
    13261 ttccatgatt tctttcgtaa agacgttgac gatcttagca tgcttgatca ctaggtcagc
    13321 aggctttttt ttacttgcta cttcgatata tttccttaat gttgtttcca atggagtgcc
    13381 tcccaagata aatttttata agtataacat aaattcggtt tttgccaata tttttttgta
    13441 aattcatttt atttgaaaca aaaagatcaa aaagacgtat gttttttgtg gaggggagaa
    13501 taatggaaat aaaacgatta tcggatcaaa tgataagtga aagttttgcc aaacatgccc
    13561 aagacgtata tatattgcca gcttcagaag tattttacat ctattttcgc tatggaaaaa
    13621 aacgaacatt ctggaagaaa gtctcttcgg cagatggaca gcaactgatc tcaagattta
    13681 agtatttggg acagatggat atcggagaaa aaagaaaagc tcagttaggt gcatttactt
    13741 attctttaga aaaacaggaa gttcgtctgc ggttgtcttc tgtaggagat taccttcaac
    13801 gagagagttt agtgattcgg ctattatacg gtacttttga aacaccccat cgttgcttta
    13861 gttctcgaga ttttgcaact attttacaga aaaccgtcag acgtggatta taccttttca
    13921 gcggaccagt cggatcaggc aagacttctc taatgtatcg gttggctttg gaagaaaaat
    13981 tgcaagtgat tactatcgaa gatcctgtag agatagaaga accgtttttt ctgcagctac
    14041 agatcaatcc gaaaatagca cagacttatg atacactttt gagactcgca ttacggcatc
    14101 gtccagactt gctaatcatt ggcgagataa gagatgaatc aactgcacat gctgcggtaa
    14161 gagcagcact aacagggcac cgggtttttg caaccgtaca tgcacgcaat cttgaaggta
    14221 cattggtcag aatccatgaa ctgacaaaga aaaaagaaga gctatctgaa tgtttagctg
    14281 gtgtcattta tcaagaactt ttggaagatg ccaatcacca tccttctgtt ttatggagtt
    14341 atcaatttat ggaggaagaa tcagtaaaaa aatcatggga ggaaagttat gaagaagctc
    14401 aggaaaatat ttggcagtca gaactattct aaaggattaa caagaagcca gcaaaaccag
    14461 tttgcacaaa ccatgggaga actgctttta tctggtttta gtctgcaaga atccctttat
    14521 ttattactca cgatccgttc gtttccacct gttattttgg caaatatcca acatcagcta
    14581 aaggaagggc aagcgtttag tgaggtactt tcgcaaatag gatatggagc agagcaactg
    14641 gtacagatcg agttagctga acgtcatggg aatctgccga caactttgtt ggaaattgcc
    14701 aaacagtttc ggcttgttga aggatttcgc aatgatttga aaaagctgct tgcatatccg
    14761 atgctattgc tcaccttttt gctaggtatc ttgtttagta tgcggttgtt cgttttgcct
    14821 cagcttattg gttcaggaat ggtcatgcaa gagcattggg gtattcagct gatacaagtc
    14881 tttcattggt atgttctact attcatccta gcggttactc tattggcttt actagtaagg
    14941 tatcgtctga tgaagttgtc tcctgttgat cagagtgaat ggataagcag acaattattg
    15001 attggaccga tatattcctt gtatcaatcg gcttatttgt cactagagat tggaaagctt
    15061 tttcatgaag gtttcgaatt aaggcaaatc atagtctgct taaaggaaac gaaacaagaa
    15121 agtctgattc agcatcttgc ctctaagttg attcaaggat tggaggcagg tatgtcattg
    15181 gctgagcagt tccagaatta tcgttttcta tcagaagaat tcagtaaaat tgttttacag
    15241 ggagaggcaa agggaagttt aggtaaagaa ctgatttttt acagcgaatg gacaagaaaa
    15301 cagcttttca aacgtcttca tcactggatg cattttattc agccaatcat ttttttgggc
    15361 gtagcggctt tgattttatt agtttatgcc gctgttttat tacctgttta cggaagcata
    15421 gaggaggttt tgccatgaaa aagagaaaaa tatcttatta tagcggtttt acattgatcg
    15481 aaatgctaat cgtcttactg atcatcagtg tattagtgtt gttgttcgtc cctaatttgt
    15541 cgcgttatag gaatcatgta gaccaagaaa gtcgtgaagc gatcatccag ttggtagaca
    15601 cacaaaaaga gctctatgcg cttcaaaata atggaagagt tcctactgtg gaggaattgt
    15661 tgaatgaagg ctatatcaaa cgagaacatg cagaaatcta tcagcggcct tgaaggtaag
    15721 gttttagcat caggatatac gttgtttgaa tcactcttac tgattttatt acttacaacg
    15781 atcctttctt ttccagttct cgcattttct gcatggaaga atgaattggc agtctatcaa
    15841 tttttcggcc aatttgaaaa acgaatctat tctacgcaaa aaatcgctat tgtgaatcag
    15901 atccaaacag gcttttattg gaatagtgaa gagaatcaaa tcatttttca tgttccgaat
    15961 ccagataaaa taaattggga agtattagat attcctgatg agattacctt gaaacgccat
    16021 gcttctatta cttttgctgc tagaaccggg aatgaaagtt cactaaaagc ttatcaattt
    16081 tattgggaag aaaaagagcg gacgattacg tatcaattcc agatagggag tggtcgctat
    16141 acaaaaagaa ttgaataaaa caggatttgt tttgttggag tcccttacag cgcttatgat
    16201 cgcttctgtc tgtgtctact tagtgagctt tggccagctg tttttgcttc aggcagaaaa
    16261 gaaaagtggg gaggaactgc tatttattcg aatgatttat gaagatatca agacgcagcg
    16321 gatgtatcac aaaagaccag agcgaaggga agaaatgatc ccatacgata tccaaattgt
    16381 cgataataat ggagtaatcg aaaaagtgtt tgtcaatatg gagaaagaag cgatcagtat
    16441 tgaaaaaaca agtaattcca gcatttacgc tgattgaatg tctaattgga ctgatgatat
    16501 tgagcagtct attgctcaca ttcaatcttt tgattcagca aaccatccaa gtaagtgaat
    16561 ttttgcagcg taaagatcaa aaagagtggc taatattttt agcacaatta gaagaagaac
    16621 tcaagagcag tcacaatgtc tctgtgaaaa atcagcaact gcactacacg atcgaggata
    16681 aacagtatat catcgaaaga tatcaggcaa tgatacgtaa aagaagaact tccggcggac
    16741 atcaacctat gttaacaagt atcactgagc ttcaattatt ggaaaaagaa cacacaattt
    16801 ctttttatgt acactttgaa aatggagaag atggatatgc catatggaca aaaaacgatc
    16861 agtagaaaat gttataaggg ctctatttta ttttctgtct tgcttttatt tcttttatgt
    16921 acgtttttat tgatggcagc attagaaagc taccgtttgt caatggatct taatatacga
    16981 atcaaaaatt attatatagc aaaaataatg aagcagatgg ctttatcgga acagtctacg
    17041 cttgccgaaa gttcggaagg agttttttac tacacaactg gatctgtaac atatcagtgg
    17101 gtacaacagt ccctattctt agaggtggaa gtctcaccgt ttatctttcg gttcacagaa
    17161 gaagtaaaaa atgatacaga tactagtaca gaatgatgga atgttcgtga aaacgccctc
    17221 ttttctgtta taacactgtt tttaataaaa aaatgtttgc cgtttcacaa ataaaaaaca
    17281 gtgaaaacga ttgaaatttt gtcaattgtt cgatagaatt aaacaggtga aaaaattgtt
    17341 ataatatatc tagaaatgag gtgcaccatg ttaccagaaa aaatggaaca agggtttaat
    17401 caaaacctgg aagctattca gcttttacag aatgcattgg gaacttcctt tttagaagcc
    17461 tatgtcgaaa atgcagaaaa tttaatagat gattatcaag tacgtgtggt ggatggagtg
    17521 ccgacaaaag agacaacgca acggatcaca gctctttatg aagagttgaa gaaactttct
    17581 ttcgagccag aagaatggcg cagactgtct caattacttt tattgaaggg cagccagaca
    17641 gaacatttgc aggcgaacca tcaactcact cctgatagta tcggtttttt atttgttttt
    17701 cttattgaac aactatacac aaataaaaaa gcaccggtta agattctgga tattgcggca
    17761 ggaatgggca acttgttatt aacagttctt ttaaacctat cgaatgcggg gtaccaaaca
    17821 gaaggaattg gtgtggatat tgatgataca ttgttggctg tcgcagcttc tacaagtgat
    17881 ttgacacaag caaatgttca atactttcat caagacgggt tgcaggaatt attaatagat
    17941 cctgtcgatt ttgcaatcag cgatttgcca atcggttatt atccgaatga cgaaaaagca
    18001 aaagagtttt taacaagtac cgaagaaggc catagttatg cacatcattt attgctagag
    18061 caatcaatga aatatgtgaa gccggatggt tttggtttgt ttttgatgcc gagtggattt
    18121 ttagaaacgg atcaaagtga agaaatcaaa aaatggttta aagaagaagg ttatcttcaa
    18181 ggaatgattc aattgccaga tgaattgttc cgtaataaac aatcgcaaaa aagtattttg
    18241 atcttacaga aaaaaggacc acaagcacaa caagtaaaag aagtcttgct tgtgaaactt
    18301 gcttcattaa aagaaccaga gaaggtgacg gaatttttca acgagtttaa aaactggaaa
    18361 tcatctgctc tgtaatcatt caacgagaag gagagtcata tgtctaaaac aattgcaatc
    18421 aatgctggga gttcaagttt gaaatggcaa ttatatcaaa tgccagaaga aaccgtagtt
    18481 gcgaaaggga tcgtcgaacg tatcggttta caagattcta tttttaccat caagtacggc
    18541 gataatcaaa aattcgaaca aatcatggat atcgaaaacc acgaagtagc agtaaaaatg
    18601 ctgttagatc agttgattga actgcaaatt ttaagctctt atgatgaaat caccggagta
    18661 gggcaccgtg ttgttcacgg aggagaaact tatggggatt cagtagtgat cgatgagaaa
    18721 gtaatggacc gcattgctga attagcagaa tttgctcctt tgcataatcc tgcaaactta
    18781 atggggatca aagcgtttaa aaagatttta cctgacattt tgagtgtggc tgtattcgat
    18841 acatctttcc actcaacaat gccaaagcaa aattatttat atagcttacc ggtgtcttat
    18901 tatgaagatt tcaaagtaag aaaatatggc ttccatggca caagtcatcg ttatgtgtcg
    18961 gaacgtgcgg caaaattatt gaacaaaccg atcgaagaac taaaaatcat cacttgccac
    19021 ttaggaaatg gtgtatcgat cacagcagtc gatggaggta aatccatgga tacttctatg
    19081 ggattcacac cattagctgg ggtgacaatg ggtacacgtt caggtgatat cgatccagct
    19141 gttctcccat atttaatgga aaaattagag attgatattg atgaaatgat caatgttttg
    19201 aacaagaaat ctggtctgct aggcctaaca ggattgtcta gtgatatgcg tgatcttgaa
    19261 aagaactatg aaaaagaaca tattcgttta gcttatgaca ttttcgtgga ccgcatccga
    19321 aaatacatcg gcggttatgt gacaacgatg aatggtgttg atgcaattgt ctttactgct
    19381 ggtatcggcg agaatgacgg acatgtacgt agtgaaatca tcaaaggtat gtcttggttt
    19441 ggttgtgaaa tcgatccagc gttgaatgaa ttgcgtggtg aagaagtaga tatctctaca
    19501 aaagaatcaa aagtaagagt attagtgatc ccaacagatg aggaactaat gattgcccgt
    19561 gatgtggagc gtctgcgcgg ctaagccaaa gcattcgtta ggataaaaga tgaaacgcat
    19621 gcaagaggga attcttgcat gtgttttttt tgcggagaag accgcaaaat ggtaatttaa
    19681 cttcaatttc ccttcttttt tacaggatct tcttagggaa ttttgcttgg aaaagtggtc
    19741 aaaagggaaa agactggcat tttatttatt gattttcagg aaaatcccaa tattttattt
    19801 tcattcatct ttataaggga cattacatat gtaaaaagaa aaaacatttt tagatcagcc
    19861 tcgtgggctg tcgactttat tcttcgcttt atgtttagct agaaaaccga tccgaaacat
    19921 tcattgatcg aataataaaa acttgatttt ccacctgtac tttgcctgaa tcgtgaaagc
    19981 acaggtatga aaatcaagtt ttttgttgcg aaaaaacgtc ctagtcaatc agttggccga
    20041 gcggctggcg gctgatctgc tcctgatcaa taacgagtgc ttcttggaag tgttgctgca
    20101 aaaagtagcg gtcatggctg actgctagga gcgttccttg atagtcttgc agcatttctt
    20161 caatttcttc tcgagcatag ctgtcgaggt gattggttgg ttcatccaaa atcaggacgt
    20221 tgattttttc taaaaagagt ttcagcaacg ccaaacggat tttttcacca ccggataaat
    20281 ctttgatccg tttacccaca tcgctttgat aaaagccact tttggctaat tgccgtcgag
    20341 cgacttcttc gtttccagtc aatgtgatgg cgtaagccag caaacggcta tcgggatcgg
    20401 caaactgcag ctgctgcggc aaataaccga tttgcaagct actacctaat ttgatggtgc
    20461 caccgtaagg cttttcctca ccgagtaata atttgagtaa ggttgatttt cctgcaccat
    20521 tgtcgccgat gatggctaaa tgatctccac gatagactgc aaaactgctg tctttgaata
    20581 gtacttgatc agcgtactgc tggtgcaagt tttgaatgat gaaaacttct ttgccagatt
    20641 ttccaccatt agacaagctt tgcagccgat ttttcggggg aggtggcggt ttgacgagtg
    20701 tcagtttcgc caggcggcgt tccagttctt tggctttttt gaagaaggac tcattgtctc
    20761 cttcatgtgc ccactgacga aaacgacgaa tcatgacttt caaccgctgg acttcttttt
    20821 gctgcagttc gtaatttttt gtcagctcag ccaagcgagc ttctttcaag actcgaaaac
    20881 ggctgtagtt tccgggatac tcaatcaatg caccttcttc cagttcccat gtttttgtgg
    20941 tgacttgatc gagaaattgc cgatcatgag aaacgaccag aaccgatttt tttgtattct
    21001 gtaaatagtt ttccaaccag cgaatactgt taagatctaa atgattggtc ggttcatcga
    21061 gtaaaagtaa atcggcatcg gctgctaaaa ttttggctaa agcgacttta actcgttcgc
    21121 ctccgctaag ttctgttaac cgtgcttcct gacggtgtgg aatccctaag ccttgcaaga
    21181 tcgctgcaat ccggtcttct aatacgtaac cgcctagttc ttcaaagcga tgctgcagct
    21241 caccgtaacg ggccagacac ttctctaagt ttgtatctgg ttctgtcatc aatgcttcgt
    21301 agcgactcat ttgctgtttg aggtcctgta tctcagcggc agaatggagc aagtagtggt
    21361 tgacggtcgc ttcggaaact gtaagttctt gttcgaccgt cccgatcttt aagccttttt
    21421 tgcgactgat aacaccgctg tcgacacctt ctctgccggt caagatttgt aaaaaggttg
    21481 atttgccagt tccgttgata ccgactaagg caattttttc accagcttca atttgacagg
    21541 ataaatcctc aaataatgga ttcccgccga ttgttttttt aatagaaatt gcttgtatga
    21601 tcatgatctc tctccttgag accactcaaa aaggccatgg ttatccatgg cctgtgttat
    21661 ttaaactcag attatcatga gaaaaatacc ttgagatggc cattgatttt acgtgaattc
    21721 aactgttttg gacaaacttg tcccgtcaac agcaactgta aaaccaattg catggctaaa
    21781 tgcgatatgg tatcgttggt aattttcatt acttgttatt ttagtcatgc gcatcatagt
    21841 attttccctc cttcgtgtag ttgtcttttc cagtgtacct gaaagtgtca gaaagttcaa
    21901 ctagcgctga catttactta atatttatcg atgtgtaatt ctgtcagaga gcttttttta
    21961 tctgactgag ggtaatgtct cgtggaccac atcattgtat tctgagctaa accagcgttc
    22021 aggcgaaatc atatggtgtt tgataaaaaa ctttgtctac atgacttcct tttgccattt
    22081 gcttaatacc gttgcattaa acagctcttc tctttggaaa tctcaccaac atcgcgttca
    22141 ggagtatcgt tgtggttgca atggcaagcg gtagcctttc atcaattctt gaatgattta
    22201 ccgaatgtag cacttgcctt ctcgcggtac ccgaacccga aataaaattt tagtttggcg
    22261 ataaaatcgg tatccgccat cgatacttca tattttttcg tatcagattc atcggaaact
    22321 tccacattga cgaaccaata agctttggca tgcttaggtc gttttaaacc acaccagggc
    22381 gactaatcgc cctggtgttc actttttttt attttacttg aactcattga ctgttttccc
    22441 tttattttaa acattataaa gaccgatgag tgcttgccgc gtcttttgtt tttatcacgg
    22501 ttttttgtgc aaaaaaacct gccgatagtt ggcaggtcgt ctacgtgtta agctttggtg
    22561 tggtctttag ctggcaattg gttttctaaa tcagttcgga caacaagcac atcgcaagct
    22621 gcgtttctaa taacatattc agaaacagaa ccaataaata aacgttctac agcattcaat
    22681 cctgtggctc ctaacataat caaatctact tgattatctt ctgggatttc tctggcaata
    22741 atttgttttg gtgagccata ttctacgaca gaggttacat tattcaagcc agctttcttc
    22801 gcattgcttt cataatctgc caatgtttgc tttgccattt cagtagcttg ttctgcaagc
    22861 attccatcaa aggatgaaac ggtttggaaa gaacgtgtgt caatgacatg tgctaataga
    22921 agttctccgt tatttcggat agctacattg accgcctttt taaaagctaa ttcagcttca
    22981 tcagaaccat ctacggcaac catgattttt ttgtattgtt gtaacataag cattcactcc
    23041 tcaagtcaat cgtattgtac tcttttattg tagaacactt tagggaaaaa gagaattatt
    23101 tactgctttg gttcagcaat tttttttgca aatcggatac tgaaggtggc agaaacaata
    23161 attacgatca gtaaaaagac caaggcaata aagcgttgat tgaaaaaagc tgttaaaata
    23221 cccattacgc ctaaaacagc atatgcagct ccgtaaaatt gtaggaatcc ttgattttta
    23281 tcatttttgt tgatcaatac aaaaaagata tcctgtttat taaataaata atagctaacg
    23341 aataataata ctaaagtggc gataatcatt aaaatcctaa tcatgaacaa atcctccaaa
    23401 ataagcaaca aatagaaaaa gcaatttgcc aagagcaaat tgctttattc tttaaaagat
    23461 ggtaatctcc gttaatgccg ttgtttgtcc tgtagtattg atcccgcgaa ttcaagcagg
    23521 tgggtcccat ggtcgtagct tcgaactacc aaatgataag gcatgttacc agcagtgaca
    23581 caagacgggt cccaagatat caataaaaat gttcggtcaa cacacaaaga ggacaattcg
    23641 aacatcacag atttcccacc tctatagtag catatgaatt taaaaagagc aatgagagag
    23701 ctcgcagaaa cagaaccgtt ttcttgtcta gttttttgga ggatgctttt tccactcttt
    23761 tatacggtaa tactgttgac ccagagcctg ttcatatttc cctgtatctt ttggctgata
    23821 atactgagca ttcttcagtt tatctggtaa atattgctga ttgacccaag cctgatcaaa
    23881 atgatgagga tactgataac ccacgccacg gttgagtgat ttcgcacctt tgtaatggct
    23941 atctctcaaa tgatcaggaa catctccagc tttgccctct cgaatatcag caagtgctgc
    24001 atctaatgcc atgtaggcag aattggattt tggcgacaag caaagatcga cgacgacgtc
    24061 tgctagtggg atacgtgcct caggcaatcc caatttttca gcagctaata cagcgttcac
    24121 tgtacgagcc gcagcagcag gattacctaa acctatatct tcataaccga taaccatcag
    24181 acgccggcaa atacttgcca gatcacctgc ctcaactaaa cgggcgagat aatgaagtgc
    24241 agcatccaca tcacttccac gaatcgattt ttgaaaagcc gaaataacgt cataatgagc
    24301 atctccattt ttgtcatggg taagagcttt tctttggaca cattcttcaa tgatcgacag
    24361 tgtaagatga atccttcctt ctttatctga aggagtcgat agtgtagcca gttccagccc
    24421 attcaatgca ctccgcaaat cgccgttcgt tgcacgcgaa aggtgaagaa gagcatcttc
    24481 gtctaactgg attgcttgtt gtccaaggcc gcgttcttta tcccttaaag catgttcgac
    24541 tgccaactgg atgtcttgtt ctgttaatgg tttgacttca aagatctgtg tacggctgcg
    24601 gatcgctgga ttgatggtaa tataaggatt ctcagtagtc gctccgatca agatgattcg
    24661 gccactttct aaatgaggaa gcagaaaatc ctgttttgtt ttatctaatc ggtgtacttc
    24721 atccaataag aggatcactg taccactcat ttttgcttct tccgctacaa cttgtagatc
    24781 cttttttgaa tctgtagcgg cgtttagcat gcgaaatgca taattagtag agccggcgat
    24841 cgcacttgcg atactcgttt ttccagtacc aggaggtcca tatagaatca tggaagagag
    24901 catgcgagca tcgaccatgc ggcgtatgat ttttccttcc ccgactagat gctgctgacc
    24961 aacgacttca tcgatcgttc gaggtctcat gcgataagcc aatggctgtt gcataggatt
    25021 ttcctccttc ttgtttttac gtcccttaat tatatcatat ttagcaagat gcctagaaag
    25081 aacagccttc tgttgttttg ttttcatagc ttatccaact aatattgtgt atgataaagt
    25141 aggtgagaaa aatgacggat aaacaaaaga tagatgaatt tttttggaat aaaacgacgc
    25201 cagaagtggc acgggattta ttggggatgt atttggagca tgacacacca gaaggaagat
    25261 tagctggata tattgtagac gcggaagcat acctaggtcc agaagacgag gccgcccata
    25321 gctatggttt aagaagaaca ccgcgtgtgc gggcaatgta tgaaaaacca ggtacgatct
    25381 acctttacac gatgcatact catcggattt tgaacatcat tactcagcca gaagggattc
    25441 ctcaaggagt aatgatacgt gcaatcgaac ccgccgcaat gattgatcaa atgagtaaaa
    25501 atcgtggcgg aaaaactggt ccagatatca gcaacggtcc gggaaaatta gtggaagcat
    25561 tagcaattcc tcaagaactt tatggtcagt ctatagcaga tagttcgtta cgcttagttt
    25621 ttgagaaaaa aaagacacca aaaaagatca atgctttgcc tagaatcggt atcccaaaca
    25681 aaggtgtatg gacagaaaag ccgttgcgtt ttgttgtgtc gggaaatcct tatatctctt
    25741 tacaaagaaa aaaccaaatc gagaaagatt ggggttggag gaaagaaaat gaaaaagaag
    25801 gatctattaa catatttaga tgaaataatt gaaaaacaag tgacagacta tgatgtagct
    25861 ttagattggg atacaaaaaa tcatacgatc gagatcgttg ttcgcttgtt tgcagaaaat
    25921 aaagcacaat ttgagattga tgatgcagaa gggatcgttt cgcaagaacc aattatcgaa
    25981 tttgaagacg gcgtacttct ttttaatcct cagaaatctg tttttgatga acgcaattat
    26041 ctagcagtaa tcccttatga aggaaaaaaa gggttggcaa aatcagtagc tgattcttta
    26101 gtggaatatc taaatgaagt attagctcaa gggcaaagcg atttgcttga ttttttggat
    26161 gaagacgatg acgaagctgt attcgagctt cactgggata atcagcaact agcagaatta
    26221 gtagaagtaa aacagcaaaa tgaagcagat acttatttac cgtacccaag ttattaagga
    26281 gtgacagata tgaaatggaa tgaagtaaaa gtagaaactg aaagtgaagc agtcgaagca
    26341 gttgctaata ttttgatgga ggcaggggcg agtggtgtcg cgattgaaga ttctttagat
    26401 gttgaacatt ttcagtcgga tcaatatggt gagattttag ataaaaatga ttttacatcg
    26461 ctgaaagaag gcgcactagt catggcttat tttccggaaa cgactttctt accggaaatc
    26521 ttgcctttta tcaaagatcg tatcacacaa ttacctgagt ttggtctagc tattggaaaa
    26581 aataaagtaa cagttagtga agtagaggaa agcaactggg caacagcctg gaagaaatac
    26641 tatcatccgg tgcgtattac gcgaatgctg acggttgcgc caagctggga aaactaccag
    26701 acagaagatc ctctagaaaa aattatcaca ctagatcctg gtatggcatt tggcacaggc
    26761 actcatccca cgacttcctt gacattgaca gcactagaaa cagtcttgcg tggtggtgaa
    26821 acattgctag atgtgggaac tgggtcgggc gtgctaagta ttgcagcaaa atatcttggt
    26881 gcaaaagatg tctatgcctt tgatttagac gaagtagccg ttcgttcggc gaaagaaaat
    26941 atggatatga atgaaatagc caaagatgtc catgtatcag ctaacgatct tttgacagga
    27001 atcgagattg aaagtgatgt aatcgttgcg aacattttag cagatatcat cttattgatg
    27061 atcccagatg cttggcgttt actgaagcaa acaggcacgc tgatcgtttc aggaatcatt
    27121 gaagagaaga agcaaatggt tctggatgcc atgttagaac aagggtttat cgtggatcaa
    27181 attttccagc aaaaagactg gtatgcaatt attttgaaaa aaccagaggt ggaatagatg
    27241 cagcggtatt ttttaaaaga atcatatgaa tcaaaagact attttgtttt agaaggggaa
    27301 aactatcacc acattgtacg ggttatgagg atggaacccg aggatgaagt ttttttggca
    27361 ttcaatgatc aaatagccat tgttgcagaa atcacggaga ttactgaaga ggctgtttat
    27421 ctgaaagaaa ttaaaaaaga agaaaatcaa aaggaactgc caagttcgat taccatagcg
    27481 agtggctatc ctaaagggga aaagctggat tggatcgttc agaaatccac tgaattagga
    27541 gcgcaagctt ttcttggttt cccggcatca gcctctgtcg ttaaatggga tgcaaaaaaa
    27601 cgtatgaaac gacaacaacg gcttgaaaag attgctcaag aggcagcgga acaatcccat
    27661 cggcagatcg tgccatcagt agagttattg gaaacgaaac aagatttatt tgagcggcta
    27721 gatgattatg acgtggtctt ggttgcttat gaggaatcag caaagcaagg cgaaaaatca
    27781 aatgcagcca aaattcttag tgaaacaaaa ccagatagtc gaatcttagc tgtctttgga
    27841 ccagaaggtg gattatcccg cgatgaagtc gattattttg tagaacacaa agggatactt
    27901 tgtggattag gaccaagaat ccttcgtaca gaaacagcac ctctttattt attaagtgct
    27961 gctagctatc aatgggaatt aatgaatcct tctttcagct aacgttcttc attaataaaa
    28021 gagtatcgac agaaagaatt gaaaaaatag tcaacaattt gtataataga aacaattatt
    28081 tatacaatgg atacgaaaaa tcgtttgaaa aagcttttaa aaagcgaaca tttccgtaaa
    28141 aaaacgtata actgcacgga ttttgggaca attatcaaac agatatttgt cccgattccc
    28201 gttttaccga aaaaagaaag ggggatcgat tcctgtgaca aaagaagaaa ttttaacggg
    28261 acccggtgtc ataaagctcg ttagtcaata catgggtccc gaacatgtag catttgttga
    28321 gaaagcatgc gaatacgcga ctgctgcaca tgacggacag tttagaaaat caggcgaacc
    28381 ctacattatt catcctatcc aagtcgcagg tatattagca gatttaaaaa tggatcccca
    28441 tacagtggct acaggcttct tacatgatgt tgttgaagat acagaaatca ctttagaaga
    28501 tctgagagaa gaatttggcg atgacgttgc tatgttagtg gacggcgtaa ccaaattagg
    28561 gaaaataaaa tataaatccc acgaagagca gctagcagaa aaccaccgaa agatgctgct
    28621 agcaatggct caagatttac gagttatcat ggtcaaatta gctgatagac tacataacat
    28681 gcgtacgttg aagcacctgc gagaagataa gcaaagaagg atcgctcagg aaactttaga
    28741 aatctatgcg cctcttgcac atcgtctagg gatcagccgg atcaaatggg aattagaaga
    28801 tacagcactt cgttatctaa atccaaaaca gtattaccgt atcgtccact tgatgcagac
    28861 gaagagagaa gaacgtgaaa aatacgtaag tggtactgtt gaagatattc gaatagcgac
    28921 agaagagctg gggatttttg cagaaatcta tggacggcca aaacacattt attcaatcta
    28981 tcgtaaaatg aaagatcaga aaaagcagtt caacgaaatt tatgacttgc tagctattcg
    29041 agtgatcgta gattcaatca aggattgtta tgctgtatta ggagcaatcc acacaaaatg
    29101 gaaaccaatg cccggcagat ttaaggatta tattgccatg ccaaaagcta atatgtacca
    29161 atctttgcat actaccgtga tcggtccggc aggaaatccg gtagaaattc aaatccgaac
    29221 acaggaaatg catgaaatcg ctgaattcgg ggttgctgca cactgggcct ataaagaagg
    29281 aaaaaatgaa aaagtagaac cagatggtat gacgaaacaa ttaagctggt tccatgagat
    29341 actcgaactt caagacgaaa gctatgatgc ttctgaattt atggaaggcg taaaaggaga
    29401 tatctttagt gataaagtct acgtcttcac accaaaagga gacgttactg agttaccaaa
    29461 aggatccgga ccattggact ttgcatacag tatccataca gatatcggta acaaaaccac
    29521 tggtgcaaaa gtaaatggca aaatggtgca gcttgattac aaattgaaaa acggagatat
    29581 cattgagatc atgacttctc caaattcatt tggcccaagt cgcgactggt tgaaattagt
    29641 tgctactagc aaagcaagaa ataagatcaa acgtttcttc aaagcccaag atcgagaaga
    29701 aaatgtgatc aaaggccacg aatccgtggt caaatgtatt acagatctag gatttacgcc
    29761 taaagatatt ttgacgaaga acaaactgca agaagcactc gatcgtttta attatcaaac
    29821 agaagatgat ctctatgcag ctgtagggta tggagaagtt agccccttga cgatggccaa
    29881 tcgtctgact gaaaaagaac gtaaagaaca aaaaatcgag cagcaaaagc aagaagcaga
    29941 agaaatcatg aatcagccga aaaaagaacc tgacaaaatg aaagtacgtc atgaaggtgg
    30001 cgttgtcatt caaggggtag aaaacttact aattcgtatc agtcgctgct gtaatccgat
    30061 tcctggtgat gatatcgttg gttatatcac taaaggcaga gggatatcca ttcatcgtcg
    30121 agattgtccg aatgttcagc ctgacaaacc aaatgtagca gaacgtttga ttgaagtcga
    30181 atgggaagat acatcgaata cacgaaaaga gtatgatgca gatttggaaa tttacggcta
    30241 taatcgttca ggcttattga atgatgtact tcaaacagtc aatgcgctaa cgaaaaatct
    30301 caacagcgtt gaagcacgga cgaataaaga taaaatggcg acgatccatt tgacggttgg
    30361 tatccagaat ttatcccacc taaagagtat cgtggataaa atcaaagcag tacctgatgt
    30421 ctacagtgta cgccggacga atggatagga ggacagatat gagagcagtt gttcaacggg
    30481 taagtaaagc tgctgtgtca gtcgatcagc aagaaattgg aaaaattggc tacggcctac
    30541 ttgttttatt aggcattcat gaaaccgata cgcaaaaaga tgtggactat ctggtaaaaa
    30601 aaattgcaca aatgcgtatt tttgaagatg aacaaggaaa aatgaatatt agtgtagaag
    30661 atgtgggagg acaaatttta agtatctctc aattcacact gtttgcggat acgaaaaaag
    30721 gaaatcgacc aagctttgtg gaagccgcac gaccagaaac agctattcct ttatacgaat
    30781 cattcaatga aggaatcaga agtcgcggca tcacagtcgc taccggacaa ttcggagcag
    30841 atatgtcggt ttctctcgtc aatgatggac ctgtaacgat cattattgat tctaaaaaca
    30901 aataaagtaa tctggttttc actagtaaaa actatgaaac acgtcgctta agttagactg
    30961 ataaactcta acttaagtgg cttttttgtc tgcaaaaagc gcttcttttg tcagcagtcg
    31021 gaaaaaaagt taagcttaaa ttatacaagt tattttttat aggaggttgc tgaagatgaa
    31081 agaagtacca gtagtagata tcaaaatcac agatttaaaa aaattgtatc aaaaaggata
    31141 caacatgttg gaggagttac gccatgaagc ggacgctccc gtcgtcaaag caaagatttt
    31201 caataaagaa gctatcacga tctacggatc atcagcagca aaagtatttt atgatccaag
    31261 gaatttcaaa cgaaaaggtg ctatgccaaa attagtattg aagacacttt ttggtcaagg
    31321 tggagtacag actttggatg gagctgctca tcaccatcgg aaaaatattt ttatggatct
    31381 aatgacaccg gaaagaatgg aagattatca tcgtatctta gataagaatc tgacacaagc
    31441 acttgaagca cagcatggtc aatttgaact atttgattta agtaagatgg ttttctttac
    31501 atctatttgc gagtgggcag gtatcaattt atcagcgatt tctaaagatg aggtcgaaaa
    31561 attagcagaa tatcaaatct ctatgatcag cggaacgttt acttctccga ttgatcacat
    31621 aaaaggtgtc gaaaatcgca aaaaatcaga aaaatgggcg caaggcttga ttgaagaagc
    31681 tcgacaaaat cctgtggctg gaaaagaaaa tgtggcatta tatgcttttg ccaatgccac
    31741 agatttagat ggtcagcttt tacctctgga agtggcagct gttgaattat tgaacatcat
    31801 ccgtccaacg gttgctctaa ctgtgtgggc ggcattgatg ggacatgcac ttttcagtag
    31861 accagatttg tatcagcaat taaaaaatga cttttcgacg ttacaagatc cgtttattca
    31921 agaaatgaga cgctattatc ctttcttccc aatgctacct gcgatttcgc tgaaagaagt
    31981 agaagtagat ggttatcgta taccagaagg cagttgggtt attttagatt tatatggaac
    32041 agaccatgat gaacgaacag ttgaagcgcc agattccttt atgatcaaac ggtatgtagg
    32101 gaaagcaaaa gatatttcct ataaagaaga atatgagatg attgcacaag gcgggggaaa
    32161 ttttcgacaa atgcatcgct gtgcaggaga atggatcact cttcatagtc tacgtgtgtt
    32221 ttccgatcag ctagtaaata agtttgagtt ctctgtacct gaacaagatt ggacgattcc
    32281 attcaatcag tttccgactt atccgaatag ccgagcgcta ctttacaaga attagattag
    32341 cataaactta tcgaaaaaaa ggaagatcaa acagtgctga tcttcctttt ttcatattac
    32401 tataaattga taaggtcaat tgatcaaaat ccacgaaatg cttcatcaat ggtttgataa
    32461 tcttcattgg ataaatgcaa atctaaagct tggacattgt cagatacttg ctctggacgc
    32521 ttagctccag gtataactac agacactcgt ggattcttca tgtaccaagc taaaacaagt
    32581 tgcgcaggag tggcttggta tcttttagca atggggcgaa gtacatcgac agcggttaaa
    32641 atttctctga aacgttctcc ttggaaatct ggatttcttt ttcgaggatc tccttcacca
    32701 aattgttttt cctcaccaag ttcatatttt cctgtcagca aaccggaagc aagaggaaaa
    32761 tagggaacaa aactgatttt gtttttttca agataaggga ataattcttt ttcagcctgc
    32821 cgatggatca aactgtattt atcttctact acatcgacat atccatctgc attcgcttct
    32881 ttcaattgtt ctaacgtgaa gtttgacaca ccgactgctc ggatttttcc tgcttctttt
    32941 aattcgtgca atgtagcaac tgattcattt tttggtgtgc tttcatccgg aaagtggata
    33001 taaaaaatgt cgatgtaatc agtttgcaga cgttttagtg catcttccac cgattgtttg
    33061 agaaactcag gagaattatc aaatgttcgc cctttatttg gtacatgagc agcttttgta
    33121 gcgatgacga cacgtgagcg atcatattct ttttccttca gtacctcgcc aatcagttct
    33181 tcggaacggc cgtttccgta agcataagcg gtatcgatca aattgatccc gctattcaaa
    33241 gctgtacgaa ctacttgttt tcctgtctca tcttccaaac cgtcaaaaag attatgtcct
    33301 ccaacggcat ttgctcctaa tccaagctgt tctgcgtaga cttgtgaatg tccgattctt
    33361 atttctgcca tagctgattc ctcctttttg tctagtctaa aacaaaatac caaaaagctg
    33421 aactaaaacg tttttgtgag gggaaatagt cagatatctc ttgactttga gactggtttt
    33481 tagtaggtta taagtaatat cgaatgtcaa tgaaagagcg agtaagtttt tgaaacttta
    33541 caagagagga tcatcgaggc tgaaaatgat ctaaagtttc attagcctaa agacactctg
    33601 gagatggacg agtgaaaagc cgtcccgcgt acgagcgtta ttcgtaagag gaaagagaaa
    33661 tctttcaaaa ttaggtggta ccgtgtatga atttcattca cccttgttca tgatgaacaa
    33721 gggtgatttt tttatttaca tagctaagaa agggagagaa aactatgagt ttccaaagac
    33781 caaaaggaac aaatgatatt ttgccaggcg aatcagaaaa atggcagttt gtcgaagaaa
    33841 cagcacgttt attattcaga gattatcagt ataatgaaat gcgtacaccg atttttgaac
    33901 actatgaagt gatatcccga agtgtagggg atacaacgga tatcgtttca aaagagatgt
    33961 atgattttta tgacaaaggg gaacgccatg tgacgcttcg accagaaggt acagcaccaa
    34021 tcgttcgttc ttttgttgag cataaattgt tcggcccaga gtatgcgaaa ccgttcaaaa
    34081 ctttttatat gggaccaatg tttcgctacg aacgccctca aaaaggacgg ttacgtcaat
    34141 tccatcaaat cggagtagaa gcatttggat cagaaaaccc tgcaactgat gtggagagca
    34201 tggtgatggc acttgatttc ttcaaacagt tggggatcac gcaaatccgg ctagtcatca
    34261 attcattagg tgataaagaa acgcgtcaag cctatcgtca agcattgatc gactatcttt
    34321 taccttttga atcagagctg agtgaagact ctaaacgccg ccttcatgaa aatccattac
    34381 gcgtattgga cagtaaagac aaacgagatc aaaaatttgt ggcagaagct ccatctatct
    34441 tagattatct aagcgagcct gctcaaaaac attttgatac cgttgtttcc atgctggatg
    34501 cattggctgt tccttatgaa atcgacagta atatggttcg cggactagat tattacacac
    34561 atacgatttt tgaaatcatg agtgacgcac caaaaatggg ggcacaagca acgatttgcg
    34621 caggcggacg ttatgacaat ctagtggaag aactaggtgg tccttcaaca cctggcttcg
    34681 gatttgcaat gggtatcgag cgattactct tgactatgga ggcagaggaa gtagttatcc
    34741 ctgcatttaa tgagttggat gcttacgtgg tcgcactcgg agacgaaaca aatatcgaag
    34801 cattgaaagt ggtccaagcg attcgaaact ttggtttttc agctgaccgc gacttcatga
    34861 accgtaaagc aaaagcgcaa tttaagacag cagataaagc gaatgccaaa ttagtattgg
    34921 tgatcggtga agatgagctt gctagtcaaa cgattaatgt caaagcaatg gcaagccgaa
    34981 aagaaaaaag ttttccatta gaagagatat atgaacattt tgatgaagtc tatgatgaaa
    35041 tgactttatt cgagtaggag gaaaaaaata aatggcacaa agaacgatct attgcggatt
    35101 agtttccgaa caacaattag aacaaacagt tacattaaaa ggatgggtac aaaaaagacg
    35161 agaccttggg ggagtaatct ttatcgacct acgtgatcgt gaaggaatcg ttcaaatcgt
    35221 ctttaatcct gaaatcaaca aagaagcatg ggaaatcgcg gataaatgtc gcagtgaata
    35281 cgtgatcgaa gtgactgggg aagttagaag acgcggagaa aatgcagtaa atcctaaaat
    35341 ggccactggt gaattcgaag tgattgcaag tgaaatcgtg attttgaatc aagcaaaaac
    35401 cccgccattt caaattgaag atgatcaagc aatcagtgac gaaatacgtt tgaaatatcg
    35461 ttatttggat ctgcgtcgtc caaaaatgac taaaaacatc aaattaagaa atgcgacaac
    35521 aaaagcgatt cgtcattatt tagatgataa tgactttctc gatatcgaaa caccttacct
    35581 agggaaatct acaccagaag gcgcaagaga ctacctagtt ccttcacgtg tccatgctgg
    35641 acatttctat gcgttgccgc aatctcctca attgtttaaa caattgctga tgaatgctgg
    35701 attcgaccgt tattatcaaa tcgttcgttg tttccgcgat gaagatttac gtggagaccg
    35761 tcagcctgaa tttacgcaag tagatattga aacaacgttc ttaacagcag aagaaatcca
    35821 aacttacaca gagggattga ttgcgaaggt aatgaaagaa gttcgcggta tcgaagtcac
    35881 attgccattt cctcgtatga cctatgatga agcaatggct cgctacggca gcgacaaacc
    35941 ggatacacgt tttgatatgg aattgatcga tctaagcgat acagtcaaag aagtagaatt
    36001 caaagtcttc caaatggctc ttgaaaacgg cggtgtcgtg aaagcattga atgctaaagg
    36061 cgctgcagat cgctactcac gaaaagacat ggatcagtta ggtcaatatg taggacaatt
    36121 cggagctaag gggcttgcat ggctgaaagt agaagaagac ggattgaaag gtccaattgc
    36181 taaattcatg ggagaagcaa cagaagcaat cattaaagca acagatgcaa aaccaggtga
    36241 cttgctgatg tttggcgcag ataaaagtga aatcgtcgct gctgcattag gtgctatccg
    36301 gacacgttta ggaaaagaat taggactaat tgatgaatcg aaattcaatt tcttatgggt
    36361 aactgattgg ccacaatttg aattttcaga agaagaaggt cgatatgtct ctgctcacca
    36421 tccattcacg atgccaaaag aagaagatat cccgttattg gctgaagacc ctgcaaaagt
    36481 ctatgcagaa gcttatgata tcgtcttgaa cggttatgaa ctaggcggag gttctatccg
    36541 tattcatact cgtgagcttc aagaaaaagt actagaaact ctaggcttta caaaagaaca
    36601 aatggaagaa caatttggtt tcttgttaac agctttagat tacggattcc ctccacatgg
    36661 cggtattgca ttaggattgg accgttttgc aatgttatta gccggagaag ataatattcg
    36721 tgaagtcatt gcctttccta aaaacggaaa agcagcagat gtgatgagtg atgcaccaag
    36781 tatggtttcg cctttacaat tgttcgaatt gaatattgat gtaacgaata tagaagaata
    36841 gccgataaaa aggtaaaaag gatttgtcga atacgcttct tttgacaacc ttgatttaat
    36901 tgaaaaacac tttataatga agccataaca tataaagcgg ggcttttcca tgaaaaaaac
    36961 attcgaaaat tgggcttatc atgattacac gaccaagctt tcggtatcta tcgtttatgc
    37021 gttgatcgca tcagtcgcat tgaatttctt ttatcagcca ggacatattt attcaagtgg
    37081 tattactggt ctagctcaga ttttgacgac tttgtcaaaa tcactgatag gattcgaagt
    37141 accggtatca acaactttgt atttactgaa tatacctttg tttgtattgg cgtggttaaa
    37201 aatcgggaag aagtttacaa ttttcacttt tatcacagtc actttgacct ccctttgtat
    37261 ccatatttta ccggaaacag tcctatctac cgatccaatc atctgtgcgt tgtttggggg
    37321 ggctgtgatg ggactaggta ttgggctagc gttgagaaac ggcctatcat cgggcggtct
    37381 agatatcgtg agtatcacga ttcgtaaaaa aacaggaaag tcgattggtt ctatttcgat
    37441 ctacttcaat gcattgatcg tttttgttgc cggaattcta tttggctggc aatatatgtt
    37501 ttacagtacg ttgtctattt tcgtcagtgg aaaagtaact gatgtcgttt atactaaaca
    37561 aaagaagatg caggtaatga tcgtaacgaa acatccagaa aaagtagtaa atgaaatcca
    37621 aaactgtatg cgtagaggga tcacgatcat caatgaagca gaaggtgctt ataagcatga
    37681 taagcagaca gtattgatga cgatcgttac acgatacgaa ctgcctctac ttagagaagc
    37741 aatgaaagct tctgatccac aagcttttgt aagtattgca gaaaacgtcc agatacttgg
    37801 tcgtttctat gaggatggca tttagatcga gtttgacaaa aattcatgaa atactgctgt
    37861 agacagacgt tcgtttgtct gcagtttttt tctaccatag tagaatcagc aaagaaaaac
    37921 tgctttttta tttgaaattt tcttaaagtt gaaaaaaagc atgtttattt ttttaaattt
    37981 atgggatgat agaaaaaaat agtagagaaa ggacacgatt ctacttgcta ttttcactta
    38041 atacccgtat attacgaatg aaaaaagttt atgaaaagtt ggaatattga atggaaaaac
    38101 aaaaaaggtt aaaaaacagc cgctacatca ctgggtttga tgggctccgt tcattagctg
    38161 tggtgggtgt tatcttctat caccttctgc caactagtat gaaaggcggc tatttgggcg
    38221 ttccgatttt tttcgtcgta tctggttatt tgatcacgga tctgctgcgg caggaatggg
    38281 aacaaaatgg aaaaatcaat atttggcaat tttatgtcag aagaatgaag agactctatc
    38341 cggcacttgt ttttatgctt gtgacagctt cggcttatat cactttgttt cagcgagggc
    38401 tgttaaacaa tcttcgagga gttgtggtca gcagcttact gtatgtcaac aactggtggc
    38461 aaatcaataa cggtttatct tatttcgatc gatttgctaa tgagtcaccg tttacgcata
    38521 tctggtcttt agcagtagaa ggtcaaaact atttggtttg gccgatcttg tttgtattgt
    38581 tgatggtatt tgttcgtaaa agaaaatgga tcttttatac tgtgttaggt acatccttcc
    38641 tctccgccat cttgatgatg atcttgtata caccaggagg cgatccaacc cgtgtttatt
    38701 acggtaccga cactcgtctg ttttctatat ggttgggaag tgcgcttgcg tttgtttggc
    38761 caagtacgcg tttaaagaaa aacatcccga aacaagcgaa aaatattctg aatatcgctg
    38821 gaggaatctc gcttttggca ctgattcttt gcttcttctt tttagatgat cattacaatt
    38881 tcatctatta cggaggaatg ctcctagtga gtctgttatg cgtagtgcta gttgcagtga
    38941 cagcacaccc cggagcaagt ttgaatcgat ggctgacaaa tccagtattt acttggattg
    39001 gcaaacgaag ctatggcatt tatctgtatc agtttccagt tatgattttt tatgaagcaa
    39061 aggtgactga tattgccgat catgttttgt tgcatacttt agtggaaatc acattgattc
    39121 taatcatcag tgaactttct tatcgatatg tggagcgtcc gctagcacgt tttgattatg
    39181 gtcgtacatt ccaagtgatg aaaggatggt tcagcaaacc gatcatcagt aagagcaaac
    39241 catgggttgt tccaggcaca ttacttgtag caattgcact cgttggtttc gttacggcac
    39301 cgaaaaacgc tgtaacagcc gatcaaaaac aactgcaggc aaaaatcgct gaaagtaaaa
    39361 aattagcaga agaaaccaga aacaattcag aaaacaccac tagttcggtt gaccaagcga
    39421 tccttgataa gtacgagttg tccaaggcac aagggaataa ggcacaaaaa ttagaactaa
    39481 cggcatttgg tgattcagtt atgttggatg ctgcaacgga tctacaagaa ctttatccaa
    39541 aagtagtggt agatggagat gtcggacgac aattatatgc cagtgtaccg tacattgaaa
    39601 aattaaagga acaagatttg ttgaaagaca ctgttctgat cggactagga acgaatggct
    39661 cattctcgga agctcagttt gatagtgtga tggatgcaat cggtgatcga aaagtatact
    39721 gggtcaatgt ccgagtaccg acgaagcgct ggcaaaatga cgtgaatgcg atgcttgaac
    39781 agatgacgaa aaaatacgac aatatgacgc tgattgattg gtacagctac agtaatgacc
    39841 atagtgactg gttttatgat gatcaagtcc atccaaatcc tgaagggatg acacattaca
    39901 ttcacttagt cagcgaagcc ttgctaggag gaagttcaag tacgaacagc agtgagacaa
    39961 acgtgacaga atgatagcta caaaaaactt caggcaaaaa catttgtttt tgcctgaagt
    40021 ttttttatat ttaccgtgtt tttaacattc atatagattc aggaagcaaa acggttgagt
    40081 gtgtctgtaa ataacgttct ttttgttttc gggtgagttt tttgaatgca gcttctgctt
    40141 ttgttgcttc gcttcttgaa gaaaatactt ctgcatggat catcttaaca ggtcttcttt
    40201 tttgaagacg agtatattta gcaccagtac cattgttatg ttcctctaat cgtctagaaa
    40261 gatcggttgt atatccacca taaaatgtct gatctttaca gaataatacg taaaaataat
    40321 gttcatttgc catagagcat ctcacgaatc tctgcagtgt attctccatc catattgtaa
    40381 gtgatcaatg gtggagcgac tttgaatccg tctaatttgc cttctttgat tccttcaact
    40441 aacaaaatat ttgcctcttt tccaggtttt gggtaaacaa attggatcct tttaggtgca
    40501 attcgatagt cttccatgac ctgaatgatt tctaagaacc gatcaggacg atggaccata
    40561 gtaaagcgtc catttgtttt tagtagctta cttgaaatat cgatcacttc ttctaacgtc
    40621 gtatgaatct catgacgggc gattgccagg tgtttattag ggttcgtttt atttgttggc
    40681 agccctttga aataaggtgg attacagacg actaagtcac aagaattatg tcgaacgaca
    40741 gataagctgt ctttcagatc gatggtgtgg acagtcattt gttcatttaa atggttaagc
    40801 gcgatactac gtcttgccat gtctgctaga cgaggttgaa gttcgatagc gtcgatagcc
    40861 gcctgggttc gtttacttaa aaaaagtcca actgcgccat ttcctgcaca aaggtcaaca
    40921 atcttccctt ttttaggtac acgggaaaag tgagcaagca gcacagcatc taaagaaaaa
    40981 gaaaacactt tggaactttg gataatcttg atatcttctg cgtataattg gtcgattcgt
    41041 tcgtcttcgt gaagcatgga ttccctccaa aatgaaaaaa atagtcgtct gtattatact
    41101 atttttatta aaaagaacca aggtggcttg tcaaacctct attttttatg catactatga
    41161 ccataagaag ggaaaggaag agactatgtt ttatcgattt atgcgcgggg tagttcgagt
    41221 actattagcc ttagttaatg gaaatgcccg ctatgagaat aaagaagttt taccgcaaga
    41281 tgagaattac atactagttg ctccccatcg tacttggtgg gacccattat atctggcggt
    41341 agctgcagca cccaaggaat tcagctttat ggcaaaagaa gaactgttca aaaatccagt
    41401 tctgcgattt atattaactc gatccaatgc ttttccagtt aaaagagaaa atccaggacc
    41461 aagcgtcatt aaaacacctg taaagatttt gaaatcgaca aatttgagtt tgatcatgtt
    41521 tcccagtggg acaagacatg ctacagaact taaaggcgga atggcattga tttccagaat
    41581 gtctaaagcg aaaatcgtgc cagctgtata tcaaggtccg ttgacacttg gggatctgtt
    41641 caagcgtcag agagtgacag tgcgtttcgg cgaaccaatc gacttatcag atatcaagaa
    41701 aacggataaa gaaggtatgg cggagatcga acgacgaaca caagcagctt ttgacaaatt
    41761 ggacaaagaa gtgaaccctg atttcaaata tgaagtcaaa aaaaaataaa atgataagtt
    41821 tatgcgtggg cggtcttttg aatctactgt cctgttcagt tcagactttt tcaatgaaaa
    41881 cgagggagag acgtcaagtc tgccctcttt ttgcgttcca atatgtaaaa tagaagacag
    41941 gtgtcatgta atggatcaaa gtgttataat aattagaagg acattttttg aggtgattga
    42001 atgcgttttt tacatacagc cgattggcat atcggaaaaa aactacaagg ttatgacttg
    42061 attgaagacc agcgtcatgt actatcttct atactagata tcgcgaaaga agaaaaagta
    42121 gaggcaatcg tgattgccgg agacttatat gataggagtg tcccagctgt tgatgccgta
    42181 gaattataca atgaattgat ggtcgattgg aatttaaagg aaaagatccc gatttttgct
    42241 atatcaggaa atcacgatag cagtacgaga ctttcagcgg gtacgccatg gttcagttat
    42301 tcgaactatt ttttgcgaac acagctagca gaagcatttg aaccagtcga gtttaaaaac
    42361 gtacaatttt tctttcttcc ttactttgaa ccaattgcag cgagattata ttttgaagat
    42421 gacaatatcc gaacgatcaa gcaagcggta gccaaggtga tagataaaat gcaggagcta
    42481 tttgattcag aaaaaaaaca agtattgatt agccattttt ttgtaatggg aagtgtaaaa
    42541 tctgattcgg aaacgaaaat cgaagtaggc gggttggatt cgatccctgg tgaactactt
    42601 acaccgtttg attatgtagc actaggacat ttgcataatc aagctgcttt gagacaggat
    42661 aatgctcgct atagcggttc cccattgaaa ttctctttat ctgagatcaa ccaaaaaaaa
    42721 ggcgtatgga tcgttgacct gacaacaggc ctggagttga attttaggga gatcgaaccc
    42781 ctgcatgata ttgaagaaat aacagcgagt ttcaaagaat tgctggatcc tgccttttat
    42841 cagtccgtca atcgggagaa ttacatctat atctatctca ctgatagaac gatcatcccg
    42901 aatatgatga accagcttcg gcaaatatat cctcgtattt tggggattga acgggcaaac
    42961 ggtcgagcag aacaaacaaa gcgcaaacga aaagtactag aaatgaaaga tcctcaaaaa
    43021 ttagcagaag cattttttag tgaaatgacg gaagagcagc taactgcaaa ccaagaaaaa
    43081 tggctcggag aaacgttgca agaaatcatg gaaatggaat gatcaagaat gaaaccgaaa
    43141 aaattacaac ttaaaaattt tggtcctttt atcaatgaga ccgtagattt ttcacgtttg
    43201 acagaagcac cgcttttttt gatcagcggg aagacaggag ctggcaaaac gacgattttt
    43261 gatgggatca ccttcgcact ttttggtgaa acttccggaa gacttcgctc tggaaaagag
    43321 atgcgttctt tgtttgctac accagacgaa gaaacaagcg tgactttttc ttttgaacat
    43381 cagcagatga gctatgaaat cgaacgaaaa ccagaacaag ttttagcaaa acgaaaagga
    43441 aacggaacaa aaaaacaggc agccaaagta acgttgacca tttttgatgg gaaaggaaaa
    43501 gaaataaaac aaatcacaaa acgtacagaa gcagatcagt tgatcagaga actgatgaat
    43561 ctcgatgcga aacagttttc tcaaatcgtt ttactacccc aaggagaatt ccgtaatttt
    43621 ttggtttcat caagcagtga taaagagact gttttacgtc atttattcgg tactcaattt
    43681 ttccaacagt tcaatgagcg attaaaagaa aaagcaaaac aacagcaaca aaaattggat
    43741 catttagagc aagagctgca tcttctgcaa aaaaggtttg ttttagtcaa agaagaggaa
    43801 ccagcagcag ggtttgaagc cgttttagcc caatggacaa accatcaggc aatactcgaa
    43861 aaacaaatca cggaagaaaa agaagctcta acggaacttc aggaaaaaca aaagcaacta
    43921 gaaaattcct actatcgttt tgaaagcttg aaaaaaagtt atgaagaaaa aacgatatta
    43981 ataaacaagc aaaaggaatt agctgctcaa aaagacgaaa tagaggaaaa gaaacgatgg
    44041 attgggtatt atgaatttac gcaacgatta gtcgaaccaa tccagcgcct acaagaaata
    44101 aaagttgaaa aaatagaact gtcacaaaaa agtagtgagc aaacaaagaa actagcgatg
    44161 actacgcaga attatgaaga gtggcaaaaa aatgaagcaa accgcgtacg ccgacaaaca
    44221 aaaatcaaag aagctgctca aaaactgcaa aacaaacaga tgatggtgcc aattgtcgaa
    44281 tccttacgta gtaaaaaaca agacgcagaa caattaacaa tttcattgga taaagaagaa
    44341 ttgaatgccg agaagctgta tcaaaatctc gctatctatc aggaaagaac tcgaattctt
    44401 caagaaaatt tactggatca ggaaaaactg caagaagatc gtcttgcttt tggacagtta
    44461 aaatatctag atgaaaagca gcaagaaata gaaaaacaaa aagcaaaaga cactgggtcg
    44521 ctcgcacaga taagagaaga actcaaaaaa gcaactgaag aaataacaaa aaatagtcag
    44581 ctattgaaag aacagacaga acaagcagaa cagctgaaaa gccaatgggc aaagcagcaa
    44641 attgctcgtc ttcagctttt tttgcgtcca ggggagccat gtcttgtttg tggatcaacg
    44701 gaacacccca tacaacaatt aaaacacgaa cagccaaata aggaagatat cttaaaagta
    44761 gaacaacaac tggcagaagc tgagtctgct gtagaaagaa ctcagcaaat cattgccaaa
    44821 agtgaagcac atgaaaataa tcttcaacaa caattatcag atttagaaca aaaaatcatg
    44881 gcaacgaata tacaattggc tgaacaagaa gaacacttgc aacaggcttt gaaaaaggca
    44941 tttcctgaga taacagaaca aaaaacaaaa acaactcttg tagtaatcga acaatcttta
    45001 gatagacgaa aaagcaagat ggccaatgca agaatagagc tggaagaaat aagacaacaa
    45061 gcagaaaagc tcgaacaaca attagctgtc tacaaacagc agcagacaga aaaaaataat
    45121 cagctccagc aactgcaagg agaaatcaaa agtttagaac aacaattgca agatacggat
    45181 agtaactgtt tagaagagga gatacgacga cttgaaaaac agttagaaga agactcgaat
    45241 caactagctg ttgagcagaa agcaggagtg caattgttgc aagatcttca tacgcttcgg
    45301 acacagcttg atcttcaaca tgaacaaata gaaaaaatag atgaaaaaat agaaaaggaa
    45361 caagaaagaa tagctaaaaa gttagaaagc cagtcgtatt tttcaacaat tgaagaaatc
    45421 tgtgcatttg cacaacagca agcacaatat aaagaaaacc aacgtacagt tacttcttat
    45481 catgaagagc aattagtgac agctgaccgg ctgcgtcaat tagaaaatat ccagctaact
    45541 gagacattcc cagatacaga agagatcagc caacagttag aagaaataag acgtatgatc
    45601 gaaataaagc aagaaaagat atacggttta caagaacaaa aaattagtaa tcaaaaaatc
    45661 tacgatgaat gtctttcgat ttatcacaca agccagacga aattagatga gcttacgcaa
    45721 ttacagcagc tttcgcaaac attcaacgga gaaaatccta agaagactag tttggaacgg
    45781 tatgtgcttc aggtttatct tcaagaagtg ttacaagtag caaatgatca tctccaacgt
    45841 ttaacgaaaa atcgttatca atttgagcta gcggatacca ttggcagtta tcgagggaaa
    45901 accggattag aaatcaatat ctatgatgat caggcaggta tgtctcgaag tgcgcatacc
    45961 ctgtctggcg gtgagagttt tattgcagca ctttctttag cattagctct tgccgatgtg
    46021 atccagacac aagctggtgg tgtagcaatc gatgcattgt ttattgatga aggatttggg
    46081 tcattagatg aagaagcttt ggaaatggca atggaagctt tagaaacgat cgaaagtgaa
    46141 ggaagaatga tcggcatcat tagtcatgta agagaattga aagaacgagt attacaacaa
    46201 ataagaattg atacagaagg tagcggacaa agcaaaatac gctatcaact tggataaaaa
    46261 gggagattgg taactatgaa atggagtatt ccagaaaaag tcattgcacg tggacgtcaa
    46321 tacttgcaag aaggccgagt gttatcggtt gtccctgacc aagaagaaaa cgtgtggcat
    46381 gcagaagtct tgggcagtga attgtatatg gttgatttgg atggtacagc aaaagagata
    46441 gattactgcc aatgtcccta ttgggaggaa cataaatatt gtaagcatac agttgctgtg
    46501 gaactttatt tacggtcgaa aaatctatct cgtatcatga aaaaagacca gccaatgcta
    46561 gaaccggtaa aagcaacaag tgagggagaa atcattgcaa aaggtttttc tcgtctaaaa
    46621 acacaaaaca atcctcaagc ggtgcaacct ttgattattg aataccaagt agaaacgatt
    46681 gagactaatc agtatcaccc agagctttct attttgccac tttacttgcg tatcggttca
    46741 ttggcatcac cgaaaaaaac atatatcgta aaaaacatct atgatttttt acaagcttac
    46801 acagaaaaag aaacttacac tgttaataaa cagtaccagt ttcgtctaga taaacgggcg
    46861 tttcaaaaag aagatcaaaa aatactcacg catcttgcag gaagtgctca gacgcatcaa
    46921 ttattaggta cgaacggctt acaagtcaaa ggaaaactag ataaaagata tctgcttctc
    46981 ccagtagaac aagcccgctt cctgttagag cagatgaatc agactacacg tttgcatctg
    47041 cagataaatg aacaaaacga tcaaggaata caattttccg atcaaaacaa acctttatca
    47101 ttcaaagtgg aaaaatttgg agaagattat ttgctgcaag cattgaatga ttttgatttt
    47161 ttctttatgc attatcagtg gggagttatc caagggacat tttattctct gacgaaatat
    47221 caacaaacgg tttatcaaac ttggaaacaa ttgattcgcc gattggagaa accagaagta
    47281 gtgttcaaga aaaaagaatt accgtctttg tttaaagaga ttatccctct gctgtcagag
    47341 atcggcgacg tagctattgc ccctgcagta gaagaggaag tagcagatat ccccgtcgaa
    47401 tttattttct ttttccgaaa agcaaaagga aaaatccaag ctcgagtcga tttcgtctat
    47461 ggcgaagtca tctattcgac tgacccgaag catgaggtct cgacagaaca ggcttttcag
    47521 gtgttacgtg acacagtaga agagaaacga gtacttgatc tattcagaat gtatcgctac
    47581 cagaaaaatg aaactggtta tgaacgtcca ttgccaagcg gggaagaact atatgccttt
    47641 ttccgcacag aactatctgt cttccgtcag tttggtgaag tcagacttgg aagaaaattg
    47701 cgagagttgt atttagatgc gcataaacat caaccgaaaa tcgaagtaac cgagaccggt
    47761 tcatggctcg atattcgatt cgatgtgaca gggatagagg agcaagaaat cgatcatgtc
    47821 ctgcaaagct tgctgagaaa tgatgctttt tatacattag aaaatggtca ggttctttct
    47881 tttgattcag aagaatttca acaaacaagc caagtgttgc aacaattacg ggaatcgata
    47941 cgtacagaag aaggaacgat ccatgtacct aaaaatcaag gattgatcat tcaaaatcaa
    48001 ttggaaaaaa gcaatgctac ttttagtgaa tcgttccaaa cgatggtaca agatctcatt
    48061 catccagaaa gataccaggc acagcttccc aaaggattga atgcaacgat gagagattat
    48121 caaaagcaag gttttcgatg gttgaagatg ttaggtcatt atcagtttgg cggtatttta
    48181 gccgatgaaa tgggacttgg gaaaacacta cagacaattg cctttttact ttcggaaaaa
    48241 gaagaacgga aaagcttctc tgctttgatc gtagcgcctg cgagcttaat ttacaactgg
    48301 caggcagagg tgagaaaatt cgccccttct ttaagtatcc aagtaatcaa tgggaataaa
    48361 aaagaacgtg aagaattact ggctaaagac acagatatcc gagtgacttc ttatgccagc
    48421 ttaagacagg atctagctaa ttatcagtcg caaaaaattg attatttgat tcttgatgag
    48481 gcacaaatgg tcaaaaatag cagtacgaaa actgcacaag cattgagaga attagctgta
    48541 cctcagcggt ttgcactgag tgggacacca atcgagaata atttagaaga attgtggtca
    48601 ttgtttgcaa cgattatgcc aggatttttc ccgactaaaa caaaattcag ggaacttgcc
    48661 gcagaagaaa ttgcgcaaat gattcgaccg tttattttac ggcgagataa acaaactgtg
    48721 ttgaaagact tgccggaaaa aacagagatg aatctatatt ctgcacttac agaagaacaa
    48781 aaaactgttt atttagctta cttgcgtcaa atgcgtgaag aaatcacatc aatggattct
    48841 gaagcattca agaaaaaccg gatcgggata cttgctggtc tgacaagact gcgacaaatt
    48901 tgctgcgatc ctcgtctgtt tattgaagat taccaaggcg gttccggcaa gctcgaacaa
    48961 gtaaaagatc tactggtagc tgccaaagag aacaaacgaa gagttttgct gttttctcaa
    49021 tttacaggga tgctgactat cttgcaagaa gagttagccg agctagggat ctcgacattt
    49081 tatttaagag gcagtacgaa accacaagat cggttaagta tggtagacgc atttaatgct
    49141 ggcgaaaaag atgtttttct gatttcctta aaggctgggg gaactggatt gaatctcacg
    49201 ggagcagata cggtcatctt gtatgacctt tggtggaatc ctgccattga agaacaagct
    49261 gctggacgtg cacatcggat cggacaaaaa aatgtggtag aagtgtggcg tatgatctct
    49321 gaaggaacga ttgaggaacg catggattca ctccagcaag aaaaacgcga actgttccaa
    49381 aaagtgatcc aaggtaatga agaacagttg acgaaactga ctgaagaaga catccgcatg
    49441 attttaagtg taggagaaat cgaggagtaa gagactcgaa aaagcgttca gtaatgggta
    49501 agaaagcctt tctaaagaag cgattgttta tttgatttaa aaaatagcaa ggaggctggg
    49561 attccatttt atcccagctt ccttgctatc atttttttat ttctattttt aaaggttata
    49621 caaagattgt cttcattgtc tctaacagct gatcaaaaga agaaatctcg atatcgcaaa
    49681 ttggagtctg ttcaattttg cgttctcgat ggttgaacca aagcgcttgc cagttagcac
    49741 tttttgcacc tagaacatca ttgtcgtaat tatcgccaac gtataaggtc tcttctggaa
    49801 gcatatgaaa ttctttctca gctagctgaa aaatctcttt ttctggtttt tggaaacctg
    49861 ttgcttgaga aatgagcata ttgcgggaag gaatccaatt gttcaattga agttgcttca
    49921 gtttttttgt ctgatggtca gtcggcccat ttgtgataat tcctaatggg acattctttt
    49981 ccttcaagaa atcgagggtt tttttgacct cctcatgcaa gcagatatta tcaagttctt
    50041 cctcgtaaat tttttgaaat aagaggccat cttcttccgt aatatgaggg tagtctaaat
    50101 cttttagtga ttggctgatc cgatgtgctc gcatatattc tagtgtccaa tctcctgcca
    50161 ttacttttgg gaagttttca tcactatgat ggcggaaccg gatatatagt ggatgcatat
    50221 ctgcatctga aacaagcgga acgactcttt tgaccgcgtt tcgaaatggc tgctgctgat
    50281 catacatcgt atcatcaaca tcaaacacaa ctgatttaat caaaatttta cacgcctttc
    50341 ggttttcatt gattttttca caaatccttg actattgtaa cagaaaaaat tgtctaaaaa
    50401 agctgataaa taaaggtttt tcttactata tttctatttc cttgacagaa taaggttttt
    50461 atatttcagt ttttttcact aaaaatcgtg aaacacgtca ataagtttga tgaacaaatt
    50521 aatcattatg gtagaagtcg attgatttat tttaatgcaa tctctgtgga attttcgaaa
    50581 gatttatttt tttaatacag tctcaatata actattcaaa cataaaataa cgttatttga
    50641 tttatttgtt tatagtaaat tttatttagt aatcattgct ttaaaatact acgcttatat
    50701 aacagagaaa ataagcgtta taataaagaa cagtattatt taaaatgaca gaaaacatta
    50761 ctattaaaga aataaaaaac ataaaaaatc ttaagaaatc ggtttcattt agcttttgag
    50821 ataaaataat agtacagttt tatacgctgt ttcatatgtt tagaaaattt gaattagatt
    50881 tgaaaactct aatttttaaa tgataaaaaa agcgagaatg ctggttttac tgtatattaa
    50941 ccttagataa ctttcatttt tttaaaaaaa ttatgaaaaa aaggtagcta tttaaaagtt
    51001 gattatgtat aatagaaaca gatgggagat tattatgatc ttctgttaca aaaaagtgat
    51061 tcttatcacg aaaaggagtg gtgcacgttg ttaacattat atacttcccc aagttgtact
    51121 tcctgcagaa aggctcgtgc gtggttgcaa gaacatcaga ttccatttgt tgaacgtaat
    51181 attttttcag aaccattgaa cagttctgaa ttaaaagcga ttttacaaat gacagaagac
    51241 ggaacagaag aaatcatctc gacacgttcc aaagtattcc aaaaactgaa catggattta
    51301 gatgatctgc cgttacaaga attattggaa cttgtgcaaa acaatcctgg attgcttcgc
    51361 cgtccaatca tgattgatga taaacgtctg caagttggtt ttaatgaaga tgaaattcgt
    51421 cggttcctac cgcgcgatgt acgacaatta gaattacgtc aagcacaatt gatggctgga
    51481 ttatagaagg ataatacgaa acttggaaaa gactattaca aagatagttt ttccaagttt
    51541 ttttgttttt acaaaaagta ttcttgatta gatgtataaa aaggtagtag aagatgatga
    51601 ttgcacgaaa aatagagagg gaaaattagc tgaaaagtcg gctatatagt attcgtctgc
    51661 caaaaatgtt atagttaaat aagatagctt gaaaggttga aaaatccgct ggttttatta
    51721 aattcgctta acagcgagat tttttttctt tacatttaag ctgatttcgc tacaatgaat
    51781 ctatattaaa cattgtggca aagtgaggtg tagcaaaatg gaaatggaac atattaatga
    51841 aaatacgatc cgtgtactaa ttggtcatga agatttagag gaacggggcg tttcattttt
    51901 ggatttactt ggcaatcaca aagaaatcga aaatttcttt tatagtattt tggaagaagt
    51961 tgatatcgaa gaggagttcc gaggaagcga agctgtcact ttccaagttt tgccaaaagg
    52021 agacggatta gaactgttta tcagtaaaaa tcttccggat gaagaaattt ctcaatcaga
    52081 agattctact gttgattcag aagatgtgag tgactttctt cgtcagcaga ttattggcag
    52141 cgatccggag gaagaattat ctggaagcgc cctagaagaa caggaaagac aagcaacttt
    52201 tgcttttgaa gattttgaac aagtgatcca acttgcaaca gatgtcgaac tagagtctgc
    52261 ttggactgat ttgtatttat tcgaagagcg ctattaccta gtcgttcact tctggatgga
    52321 aaatctcaac caagcggatg tagagaatca gattgctcga attttggagt ttgctgaaaa
    52381 gagcgatcgt acaccagaaa cattgaatga atatggacaa tgtctgatgg cgcgtaatgc
    52441 tatcgagcga acgaaatttt attttgtata aatcagcatt atagtaagag actgtgacaa
    52501 aaattgtgtc gcagtctttt tttcgtataa atgtgttcaa aagcttgcat ctgcggtaac
    52561 aagcccaacc aaacgaaaaa tattgggaac tatttttgtt tagctgttct tattacttga
    52621 tgcagaatag ctttttcacg ccctcttttt tcgtgtaaat aaattcttag acgtaaataa
    52681 gttcagaggg agaaaaatgc tgcttgcaat gaatgaatat aataagtgct gttttgctaa
    52741 acatgcagaa aaaggaaaga aatattattg tcgtggctgt cgaaaaagag tgattttgaa
    52801 aaagggaaag aaaaaatgtg ctcattttgc ccatcaaaaa tcagataact gttcggtctt
    52861 tagtgaaaga gaatcaaaag agcacttaca gttaaaggaa tgcttcatgg attggctggg
    52921 acagtcagta gaaccagtat ttttggaagc ctatttgccg aggttgcggc aaaggccgga
    52981 tattttgttg gcgaatctag caatagaaat ccaatgttct agactgtctc accagagatt
    53041 tattgaaaga acccaaaatt atttgaataa cagttaccaa gtatggtgga tacttggtaa
    53101 ttcttttctt ggacaatcgc aattctcatt gatagaaaaa agttgctgct actataatag
    53161 aaaaagagga gtgcattgct ggaaagctga tctcaagaaa caaaagcttt atttgtatca
    53221 tcatattaca gagactgtat cagggcatat ttccttttgc agtagctgct ggactttttc
    53281 tagtagggac cttaaagaaa tattcactag taacgaaatt aaaatgaacc aaatgaaaaa
    53341 aatagaaaaa gtgagtgaag atgggaagaa atggctgaca cgccaactta ttcacaagca
    53401 aaagaataca gtatccatac aagaacaatg ctacttgcgt cataaacatc ttttgcatct
    53461 atcatcgtgg atctatcaaa aaagtcggtt tttcttttat ttacaagaac aggtttttct
    53521 ttatcgaatg ttgtacgaag aagccttgga aaaccagaaa gttcctgatt tcaactcatg
    53581 gctttgccaa ataaaagaac ataaaaggga atggcttttt cctatgattg atgaagagat
    53641 ggtctatcag cagtttttta acgaatgcat acacttgtct tctttgaaaa aatagtaagt
    53701 ataaaaatag gctaggaatc ggttttctat gtaaaacaag atttagttat gctaaaattg
    53761 atggaagcag acacatgaat gagtagaaaa gttagatgct gtgacatgtg gatgttagac
    53821 tatgatctat tttgatagat agagctaagg gaagaaaagg agtggatttc atggaagtaa
    53881 agcagttgcc aaaacgagaa gaattgcctg aaaatttaac ttgggacttg accaagatct
    53941 tttcaagcga ccaagagttt gatgagaaat atttggaatt atcagaagag ttaaaacaat
    54001 ctgaaaaaca caaaggaaca cttgatcaag gcgcttctca atttttaaat gcgattgaat
    54061 tcgtattgag ggtttatcgc caaactgaag tcatttatgt atatgcgcac cttaaaaacg
    54121 atcaagacac tggaaataca gattaccaag cgctttatgc aagagcaagc agtctgtttt
    54181 cgaaagttag tgaagccgtt tcctggtttg aaccagaaat attgcaattg tcagatgacc
    54241 agatttggca atatttcaaa gaagaaccaa aattggaagt ctatcgccat tatatccagc
    54301 aaatagtaga taatcgagcc catgtcttat ctgctgagca ggaatctctt cttgctggag
    54361 caggtgaaat ctttgatgct tcaagtgata catttgctgt tttgaataat gcagatctag
    54421 tttttccaac gattgaagga gaaaatggtg aaatagtcca attatctcat ggcgtgtatg
    54481 gtcagttgct agaaagcacg gatcgaaggg tgcgcgaagc agcatttaag gggttgtaca
    54541 gtgtttacga acaatttaga aatacatttg cttctacttt aggcacacat ataaaaggac
    54601 ataattttaa agcgaaagtc cgtaattaca gctctgccag agaagcgtct ttgagcaata
    54661 atcatattcc tgaaagtgta tacgatactt tggtagacgt ggtaaacaag catttgcctt
    54721 tgttacatcg atacatggaa ttacggaaac gtttattaga agtggaaaaa ctgcacatgt
    54781 atgatcttta tacaccggtc ttaggggaag ctccaattac ctttacgtac gaagaagcaa
    54841 aagaaaaagc tttagaagca ctgaaaccaa tgggtgaaga atacatggcc atcgtagaaa
    54901 aagcattctc tgaacgttgg atcgatgttg tcgaaaataa agggaaacga agcggtgctt
    54961 attcttcggg aagctatgac acaaatccat atattttatt gaattggcat gatacgctgg
    55021 atcagctatt tacgcttgtc cacgaaatgg gacatagtgt tcatagttat ttcactcgtt
    55081 cgaaccagcc ttatgtgtac ggcgactact ccatcttttt agcagaaatt gcttcgacaa
    55141 cgaatgaaaa tatcctaacg gagtatttat tggaaacaga aaaagaccct cgtgtacggg
    55201 cttatgtact caaccattac ttggatgggt ttaaaggaac agttttccgc cagacacaat
    55261 tcgctgaatt cgagcatttc atgcatacgg aagatgaaaa aggtgtgcca ttgactagtg
    55321 aatacctaag tgatagttat ggtaaattga atgcaaaata ttatggtcca gcagtcgaag
    55381 aagacccaga aattaaattc gaatggtcac ggataccgca tttttattat aattactatg
    55441 ttttccaata ctcgactggc ttttctgctg cttcagcact ggcgaagaaa atactaaacc
    55501 aggaaccaga ggcactagag aactacttgg cttacctaaa agcaggtaac agcgattatc
    55561 ctgtggaagt aatgaaaaaa gcaggagttg atatgacaca ggctgcatat attgaagatg
    55621 caatgtcgat gtttgaacaa cgtctaaatg aattagaaga attgatcgat cgtttataag
    55681 gctttctatt tttgattttc tcgaatagaa acgaaaaaaa tacgtcggct ctcttgtaag
    55741 ggagtcgacg tatttt
//
LOCUS       DACECY010000012        55459 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.012, whole genome shotgun sequence.
ACCESSION   DACECY010000012 DACECY010000000
VERSION     DACECY010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.012"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(4..1230)
                     /locus_tag="IWO38_000738"
     CDS             complement(4..1230)
                     /locus_tag="IWO38_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312849.1"
                     /translation="MIFYMFININEVLKMTTITMTKNIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYRNKSKYGLLPFRGRDIKIHKADKFYISLPKGFRLKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            complement(1364..1564)
                     /locus_tag="IWO38_000739"
     CDS             complement(1364..1564)
                     /locus_tag="IWO38_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312850.1"
                     /translation="MLKRIAWDKDNKTVIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            complement(1664..2032)
                     /locus_tag="IWO38_000740"
                     /pseudo
     CDS             complement(1664..2032)
                     /locus_tag="IWO38_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2083..3711)
                     /locus_tag="IWO38_000741"
     CDS             complement(2083..3711)
                     /locus_tag="IWO38_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312851.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKEFEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            complement(3731..4624)
                     /locus_tag="IWO38_000742"
     CDS             complement(3731..4624)
                     /locus_tag="IWO38_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312852.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EDEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHEELSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNAYI
                     ARLKRFIDKYKENSDKFSKDK"
     gene            complement(4879..5199)
                     /locus_tag="IWO38_000743"
     CDS             complement(4879..5199)
                     /locus_tag="IWO38_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312853.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            complement(5232..6350)
                     /locus_tag="IWO38_000744"
     CDS             complement(5232..6350)
                     /locus_tag="IWO38_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312854.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGNSKDEVKGVVIDDDFEFDFD"
     gene            complement(6379..7173)
                     /locus_tag="IWO38_000745"
     CDS             complement(6379..7173)
                     /locus_tag="IWO38_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312855.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSKRLRITVEYGTNESATELKQRYLDTRREASKKYNDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            complement(7226..7579)
                     /locus_tag="IWO38_000746"
     CDS             complement(7226..7579)
                     /locus_tag="IWO38_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312856.1"
                     /translation="MISENLSYENNKHLVKENKKVEDYKNMYIEMKKSPLCIQYKNDN
                     WKQVVEFVKPHSIRRVRRGKKKSELVNSGRAVIRVITKDYPVEYGDYILRLSYHAFVV
                     LKEEHFNEFFNLKSK"
     gene            complement(7681..7938)
                     /locus_tag="IWO38_000747"
     CDS             complement(7681..7938)
                     /locus_tag="IWO38_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312857.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGLRSYTTSEKDK"
     gene            complement(7941..8165)
                     /locus_tag="IWO38_000748"
     CDS             complement(7941..8165)
                     /locus_tag="IWO38_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312858.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            complement(8338..9381)
                     /locus_tag="IWO38_000749"
     CDS             complement(8338..9381)
                     /locus_tag="IWO38_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HAQ0312859.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            complement(9560..9814)
                     /locus_tag="IWO38_000750"
     CDS             complement(9560..9814)
                     /locus_tag="IWO38_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HAQ0312860.1"
                     /translation="MKKEELIDIFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLNSDDVNFAHDVVGIQNHIDRENKRMGDEFLPRYSSL"
     gene            complement(10004..10219)
                     /locus_tag="IWO38_000751"
     CDS             complement(10004..10219)
                     /locus_tag="IWO38_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312861.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKKFWLNNFSYKDFIKEKANTLY"
     gene            complement(10271..11185)
                     /locus_tag="IWO38_000752"
     CDS             complement(10271..11185)
                     /locus_tag="IWO38_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312862.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            complement(11185..12084)
                     /locus_tag="IWO38_000753"
     CDS             complement(11185..12084)
                     /locus_tag="IWO38_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAQ0312863.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            complement(12084..12671)
                     /locus_tag="IWO38_000754"
     CDS             complement(12084..12671)
                     /locus_tag="IWO38_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312864.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            complement(12836..13027)
                     /locus_tag="IWO38_000755"
     CDS             complement(12836..13027)
                     /locus_tag="IWO38_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312865.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            complement(13100..14002)
                     /locus_tag="IWO38_000756"
     CDS             complement(13100..14002)
                     /locus_tag="IWO38_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312866.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            complement(14019..14495)
                     /locus_tag="IWO38_000757"
     CDS             complement(14019..14495)
                     /locus_tag="IWO38_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312867.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMHE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            complement(14511..15110)
                     /locus_tag="IWO38_000758"
     CDS             complement(14511..15110)
                     /locus_tag="IWO38_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ0312868.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLQSNIVSMGLSGGIFGWIALFAIAYIHFDSKEHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            complement(15114..15167)
                     /locus_tag="IWO38_000759"
                     /pseudo
     CDS             complement(15114..15167)
                     /locus_tag="IWO38_000759"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15818..16357)
                     /locus_tag="IWO38_000760"
     CDS             complement(15818..16357)
                     /locus_tag="IWO38_000760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312869.1"
                     /translation="MNKRRILWLIAGMYLCIAVSGFTICRIINQLKNAIVYLGEIGYL
                     EDLKAGLQIMSWILFVLTVALVILTCENFSTIKKQIISNHNQETVKSGLEVDPEEEVT
                     EQAIKEQEEIDKKEIMSIYLSSGESFFHEIDSLKLSSQEIIDRWQNMSLDGELIVEDS
                     EGWSAYKKDEIVAITILFV"
     gene            complement(16496..16975)
                     /locus_tag="IWO38_000761"
     CDS             complement(16496..16975)
                     /locus_tag="IWO38_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312870.1"
                     /translation="MKTDLTRQAEKCLWHETNRMGVFGCFEVTIGWDGRERVDYITYS
                     VDNTVRCYEIKVTLSDLKSKAKQTFIGDYNYLVVTSDLWEKMLQDDTLMRKYYKQGVY
                     VFNEKYPSMISVKKARKQKVSLGTRSTVLESMVRSMNREVKKLYKVKPFWEIVEIPE"
     gene            complement(16972..17430)
                     /locus_tag="IWO38_000762"
     CDS             complement(16972..17430)
                     /locus_tag="IWO38_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312871.1"
                     /translation="MLTILKLYREGFNEKVSIGESIEYRGDKYIIIRILKINKHYFSK
                     SISLTVRVLVQKVGDLSDYYNCKREVIVDNRYDQSKDDKRIIKIGDIVFPEKGIAYEV
                     VSINSFRYEFVDLVVEYSVRLIVPWSKEEINEALKDERKSTFKVLEGEKK"
     gene            complement(17442..18071)
                     /locus_tag="IWO38_000763"
     CDS             complement(17442..18071)
                     /locus_tag="IWO38_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312872.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKKDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNERKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            complement(18068..19444)
                     /locus_tag="IWO38_000764"
     CDS             complement(18068..19444)
                     /locus_tag="IWO38_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ0312873.1"
                     /translation="MAMKLLEKVKNRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISDTPHMLVAGTTGSGKSVLLNEILVTAICHAMPDELKLG
                     IVDPKRVEFGRYKKLPYMLADPITDMDEAYEFFEYLVILMNERYKLMEKAGVQNITQY
                     NKYAEKHGIEKFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMFIKLNGGNKIRAQG
                     AYISNEEIEAIFDHLRNTMPEPEYVDYRAIVAESRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANSSSKEPSIEKKSTFALKLEKNKEEKEKQGAKTLEKLQAEKLEKVLKALDK
                     VDPEPQTSNNTNNEQPISSKTKPKTTDPKVSVKVTITTDNSEKKQVNGTTSPIMQKML
                     KAKKQVHK"
     gene            complement(19524..20786)
                     /locus_tag="IWO38_000765"
     CDS             complement(19524..20786)
                     /locus_tag="IWO38_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312874.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVANFSKSVYLVGRALVVSAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATRGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETEKSTASIAVSNSQFAFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            complement(20820..21146)
                     /locus_tag="IWO38_000766"
     CDS             complement(20820..21146)
                     /locus_tag="IWO38_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312875.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDD"
     gene            complement(21166..22035)
                     /locus_tag="IWO38_000767"
     CDS             complement(21166..22035)
                     /locus_tag="IWO38_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAQ0312876.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGNVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            complement(22266..23072)
                     /locus_tag="IWO38_000768"
     CDS             complement(22266..23072)
                     /locus_tag="IWO38_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312877.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQFAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTAENWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            complement(23160..23960)
                     /locus_tag="IWO38_000769"
     CDS             complement(23160..23960)
                     /locus_tag="IWO38_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312878.1"
                     /translation="MVGISKTALDSLAKLFSSRFDSAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            complement(24299..25378)
                     /locus_tag="IWO38_000770"
     CDS             complement(24299..25378)
                     /locus_tag="IWO38_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0312879.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDGGSFDIGEAEGKVNPYTGDVFSKNQT
                     KDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLPLSGKGHQTVI
                     AGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQNSTLTDIPDL
                     DMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDSEYVSDKTETV
                     TSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTGITNKHQKMLL
                     VIWTVIGALASLNVLILLIKEFRKA"
     gene            complement(25368..26225)
                     /locus_tag="IWO38_000771"
     CDS             complement(25368..26225)
                     /locus_tag="IWO38_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0312880.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKIILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            complement(26300..28135)
                     /locus_tag="IWO38_000772"
     CDS             complement(26300..28135)
                     /locus_tag="IWO38_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0312881.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            complement(28184..30574)
                     /locus_tag="IWO38_000773"
     CDS             complement(28184..30574)
                     /locus_tag="IWO38_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAQ0312882.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDEQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            complement(30844..32091)
                     /locus_tag="IWO38_000774"
     CDS             complement(30844..32091)
                     /locus_tag="IWO38_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312883.1"
                     /translation="MNMKKNSLAMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNINK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            32484..32900
                     /locus_tag="IWO38_000775"
     CDS             32484..32900
                     /locus_tag="IWO38_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312884.1"
                     /translation="MNKKRKLSVLITLVSMLAILSGILSIKIFNQINTSANTKNEIVI
                     ENTSNSKEISTTKNKDKSDETTTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKDEELYSFVK"
     gene            complement(32917..33654)
                     /locus_tag="IWO38_000776"
     CDS             complement(32917..33654)
                     /locus_tag="IWO38_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312885.1"
                     /translation="MADRKLEKLLEETWDPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENRGTWDFWANLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISEQDGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVNITNRLDYDSWADIALEKYIYSTLKEFIENRMNIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(34072..34635)
                     /locus_tag="IWO38_000777"
     CDS             complement(34072..34635)
                     /locus_tag="IWO38_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312886.1"
                     /translation="MQNHEITTAKKAIGILSIILILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            complement(34622..35149)
                     /locus_tag="IWO38_000778"
     CDS             complement(34622..35149)
                     /locus_tag="IWO38_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312887.1"
                     /translation="MNSKTKDAIIISLLSGMLLAMLVGILYFNKQNQDIEAEKHSVQI
                     ENELTFFKEVKPNEVDGLQRDLENDIQEMFKGSDRSKFANQSGEEFLRKLFIVTGKPP
                     VTNESSEQEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            complement(35174..36505)
                     /locus_tag="IWO38_000779"
     CDS             complement(35174..36505)
                     /locus_tag="IWO38_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ0312888.1"
                     /translation="MVQFTGIAMSVITINPQVLHADGIATYDAQFNCTTEGSGSGTDS
                     DGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRTVEGYY
                     GDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMFKG
                     DGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMKSK
                     GMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEGKI
                     NGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFQGELQGQCTELTWAYMSQL
                     YDGNQPTLGNGGYVWESYKSAGAKVTNKPIVGYAFSALDGWLWAVGSAGHTGVVIAVF
                     DDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            36631..37107
                     /locus_tag="IWO38_000780"
     CDS             36631..37107
                     /locus_tag="IWO38_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312889.1"
                     /translation="MTDKTSDLPKLDPKISQVKSKKDGKTKNAVKETSSLLLDIPMSS
                     FDEILDANTDETSVKIASHVGMNSQNDYFYNLLNSLRKLEDKKNQVIASKAPDLTTDL
                     DAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            complement(37222..37454)
                     /locus_tag="IWO38_000781"
                     /pseudo
     CDS             complement(37222..37454)
                     /locus_tag="IWO38_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(37457..37774)
                     /locus_tag="IWO38_000782"
     CDS             complement(37457..37774)
                     /locus_tag="IWO38_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312890.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            complement(37789..38620)
                     /locus_tag="IWO38_000783"
                     /pseudo
     CDS             complement(37789..38620)
                     /locus_tag="IWO38_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(38673..39002)
                     /locus_tag="IWO38_000784"
     CDS             complement(38673..39002)
                     /locus_tag="IWO38_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312891.1"
                     /translation="MKLEGSLQNRMMEGSTTNKEIVVGMGATELLYTDRNPYTVIEVK
                     SKNRILVQADGAINKATFPDQEWEYSRNPEGQILELVKTKNGWKVLKEDIHFYIGDRE
                     KYYDPSF"
     gene            complement(39130..39375)
                     /locus_tag="IWO38_000785"
     CDS             complement(39130..39375)
                     /locus_tag="IWO38_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312892.1"
                     /translation="MKEIEGIKKRNQERLELLNENRNRNRMFSYYESMRCLNKSRSEI
                     IESSKYKQLMEERVSLIQEIETESIFPDHSPKVRFYN"
     gene            complement(39466..39753)
                     /locus_tag="IWO38_000786"
     CDS             complement(39466..39753)
                     /locus_tag="IWO38_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312893.1"
                     /translation="MVEVEFGSKEWFEELNSMIKFPEKFTFYYNKRDNIIQVALLETK
                     GRLENTRWSMIKPSALRFHPKDLTAVVAVKEVQDSQEYYEVVKNLKDIWCK"
     gene            complement(39943..40125)
                     /locus_tag="IWO38_000787"
     CDS             complement(39943..40125)
                     /locus_tag="IWO38_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312894.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENVEDLRLIGE"
     gene            complement(40162..40962)
                     /locus_tag="IWO38_000788"
     CDS             complement(40162..40962)
                     /locus_tag="IWO38_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312895.1"
                     /translation="MSKDLRELKLRKKQYNQYFSSLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSRSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            complement(41580..41840)
                     /locus_tag="IWO38_000789"
     CDS             complement(41580..41840)
                     /locus_tag="IWO38_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312896.1"
                     /translation="MAYENLQLVVAEVSGDIYLTKVKDGIMDTNYRRVATDDVLSSST
                     EWFRKNEESAVHFEGIDGNIHSLFYTKDKEKAKKILDILKGR"
     gene            complement(42031..42399)
                     /locus_tag="IWO38_000790"
     CDS             complement(42031..42399)
                     /locus_tag="IWO38_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312897.1"
                     /translation="MWNNKLKTGLLLIVISCAMMVGMIIQREQSYFEVSANNVIERCH
                     YGQHFWTGEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSNLMMKKV
                     RKLAQNYLEEKEGNSPIDKN"
     gene            complement(42561..43091)
                     /locus_tag="IWO38_000791"
     CDS             complement(42561..43091)
                     /locus_tag="IWO38_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312898.1"
                     /translation="MSQNIGAVKQLIEKDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAEEKSSILEISQ
                     NDKAIQVKCVDPTGKIIVGFDIERKSKIDFITQLTKINNLNILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFSNA"
     gene            complement(43107..43472)
                     /locus_tag="IWO38_000792"
     CDS             complement(43107..43472)
                     /locus_tag="IWO38_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312899.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDQQFNQTHHNIILLVDQAKRRLEKAEYELLDQGIICLDKGIRRYYSAELKKQ
                     NVDRHALKHEILELIMQRI"
     gene            complement(43478..44221)
                     /locus_tag="IWO38_000793"
     CDS             complement(43478..44221)
                     /locus_tag="IWO38_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312900.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKAKSIRELKTEVEKIRLGLTREEISRLSENSSFVIQKEKEKHLLHRELIIEKYYDV
                     DLPDQLRIIDEFSEKKWKSEIYPSLIQYKASGKPMYNYTGFLNYTQYKYAFRKLKGIK
                     QADIALYEMKKMDRLKLIQELVQNTWNQAIVELEKENGKILTNEYLIVNEIEELIGLF
                     SCENGEVFLKSSPIYSILDSPYQFIQVRR"
     gene            complement(44352..45074)
                     /locus_tag="IWO38_000794"
     CDS             complement(44352..45074)
                     /locus_tag="IWO38_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312901.1"
                     /translation="MKSTKWKIVLLSTAALITLTACGTTNSADNNKDTKSSSTVVTKN
                     SSTSASTTISSMTENNAEKYQKYEDINKGQKPEEVYTDFINTVKNDEDYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYHAVAVDITDGV
                     PEWIQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            complement(45080..45325)
                     /locus_tag="IWO38_000795"
     CDS             complement(45080..45325)
                     /locus_tag="IWO38_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312902.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLVLNKRKKDR"
     gene            complement(45336..45920)
                     /locus_tag="IWO38_000796"
     CDS             complement(45336..45920)
                     /locus_tag="IWO38_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312903.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSIT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTIISNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIDTSIKELREYVQNPYNFQYLAGRINLV"
     gene            46042..47625
                     /locus_tag="IWO38_000797"
     CDS             46042..47625
                     /locus_tag="IWO38_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312904.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALV
                     LDLIDKLSDLANIYEDITSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLPYGNEE
                     ADRDRESTFRLLNALIFDNKEFYLKILKEEISKYRFTAESREEINKSLGENQQITYTK
                     GVFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGSN
                     NEELQNHLNSLLDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKNLYELAESKSDMYLDIIDNIINKSEDY
                     LRKEINSEKELLNYVHKIK"
     gene            complement(47882..48217)
                     /locus_tag="IWO38_000798"
     CDS             complement(47882..48217)
                     /locus_tag="IWO38_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312905.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
     gene            complement(48392..48886)
                     /locus_tag="IWO38_000799"
     CDS             complement(48392..48886)
                     /locus_tag="IWO38_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312906.1"
                     /translation="MNKLPEKNRVFRLKGDLSQFKIDENGQFFVWMRHEHCWCNPFEL
                     KKENEIRTKIANGEWDYLFRWEDESFIANDMDHAFVSFLDNTWGTWYDCYLEKLGFQN
                     IDIDAIECLKEVDQKYEEEPYYLLQQKLANNSVFQVEIWENEDGTYEGLAQEIPFSDR
                     KSSI"
     gene            complement(48883..49110)
                     /locus_tag="IWO38_000800"
     CDS             complement(48883..49110)
                     /locus_tag="IWO38_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312907.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFNEVKGMREQYEVVEA"
     gene            complement(49277..51247)
                     /locus_tag="IWO38_000801"
     CDS             complement(49277..51247)
                     /locus_tag="IWO38_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312908.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRLEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAEINVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            complement(51262..52128)
                     /locus_tag="IWO38_000802"
     CDS             complement(51262..52128)
                     /locus_tag="IWO38_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312909.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            complement(52118..52351)
                     /locus_tag="IWO38_000803"
     CDS             complement(52118..52351)
                     /locus_tag="IWO38_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0312910.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            complement(52363..54519)
                     /locus_tag="IWO38_000804"
     CDS             complement(52363..54519)
                     /locus_tag="IWO38_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312911.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAEDVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEDATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIIAVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            complement(54539..54760)
                     /locus_tag="IWO38_000805"
     CDS             complement(54539..54760)
                     /locus_tag="IWO38_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312912.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            complement(54870..55208)
                     /locus_tag="IWO38_000806"
     CDS             complement(54870..55208)
                     /locus_tag="IWO38_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312913.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNEQLG"
BASE COUNT        16069 a        10962 c         7298 g        21130 t
ORIGIN      
        1 ttactaactt ctgacttcta tcccatatct ttttcctttg tttactagtt gttctttcaa
       61 ttgatagtaa gcccattcgc cataagcctt atctggttca atgatacgaa taatttgagc
      121 tttattgcga atagcagttt taacgatttt tgcagaacat tgattattat aagttttaac
      181 aaagttactt tctcttttct ttattgcttc ccatcgatct tgatctaatt ttctttttcg
      241 accatgaccg cccttagaaa agactcctcg gctttgttct tttgatttca ttgctaaaat
      301 tttacttttg aagtttctta tatattggct atcaccaaaa gacattacag atttatcatt
      361 aattaaacat tcaccgctac acaaatctgc aaagtttacg atcaattcat tacctttctt
      421 gaattccatt tcattatagc ttttttcaaa agaaattgtt agattaaaaa ataattttct
      481 tccatcaaaa ataagattac tttgatttaa tttgtaatcg ccatccataa tgtgttgtaa
      541 aactgaagca acatctcttg cttttcttgg cttgctacct aaatttactt taagtctaaa
      601 tcctttaggt aaagagatat aaaacttgtc agctttgtga atcttaatat cccgacctct
      661 gaacggtaac aatccgtatt tacttttatt tctatagatt ctaggtgttc tttgaccagt
      721 aacaattcca ataaaatcat tgccaaaatc cttactagct ttcatatttg ctgaatcaac
      781 aaaatctcca aagtcaaagt ctaatttttt tgctacatct cgtgttctcg tttgactctt
      841 caaccctatc gcttgcgtta atatttctct cgcttcttta tttttttcat ttctcaattt
      901 agataattca gcttttgctt tatcaagttt tttttgatct attggatcag tatatgctaa
      961 cttttcaatc cgattttgaa tactttcttc tctctcagca taaccttgat ccatttcttt
     1021 aattgcatct tttatcaact cttcaaacat tagctgttta tataccagat tcaacgcttt
     1081 tacatattct ctactatttt tccaaaaaaa atcaaaaaag gaatcccttt cttcttgtga
     1141 tccatcaaca tatacttcta tattttttgt catagttatt gttgtcattt ttagcacctc
     1201 attaatgttt ataaacatat aaaagatcat aaatacgttt aattgttaag ctcatattca
     1261 catgactgtc caaatgaaat atcttatctt tattagaaat ttcactcata acacgataat
     1321 atagaccatg attcaagtat cgcaatttcg acctcaattg cttttagaat tgtaccgtat
     1381 ccagtaaatt cttacgccag tccatcacta atttttcaaa ttcatcttca tcaaaactaa
     1441 attctaatcc ttctttttta aacagtaact ttctatctat acatctcgag aaataattgt
     1501 atttatattc tttaagctca ataactgttt tattatcttt atcccatgca attcttttta
     1561 acatattacc acttaatcct tcatgtaata actgcttttt tatttgaata cgtgtataca
     1621 agttattaaa gtaaattgct gaacctatta aataaaatgc atatatcgga attaacagtt
     1681 caagagagac cttggctatt aactcagatt caactatgaa aatataaaaa aaccaaataa
     1741 atgaagggat tatccaagca aaaatttcta atatcacatt aaatcttttc atttttccaa
     1801 aactactaag acttgatact aattcaatca aaagcttatc gctacttgcc gattcatcat
     1861 atttagttcc tagattatct aaacttagca tataagaaat ttttttctga tctgttagtt
     1921 ttgaaaatga actttctttt aaactttgga ttatacaatc agcactaaat atttcgttta
     1981 ataaagcagt tttcttttct aattcctcag aagataaagt tagttctctc atagtgactt
     2041 acctactttc caaattttgt gtcctattta tcacttctct ctttatataa gtgataaaag
     2101 atcgtaaata cgtttaattg ttaagttcat attcacatga ttatccaaat aaaatatctt
     2161 atctttatta gaaatttcac tcataacacg ataatataga ccatgattca agtaatctgt
     2221 atatagaaaa atcgcatcac acgtattcaa atttttaagt atctttccat tattttcgct
     2281 tacatcaaac attttaatat ttggtaatag ttcagttaat ttattttgaa aatttacatg
     2341 tcctcccact aaaagaatgt ttttatgttt tagataagct atcatttcag acagtgaagg
     2401 ctcttcagtt ttaccattta atgattgtct atgttcttgt tcctgcaatt gctttaatac
     2461 tttattttca aacctcagct gttcaaattc ttttctgatt ccttttaatt ctttaatctc
     2521 ttgcttcaaa agttcctctt ctttttttgc cgatttgact aaagattcta accgaccgtt
     2581 ttctttactc gcaatactta attcttttct taatttgatt acttcagatt tgttttcttt
     2641 tctaatactt gcaatttgtt ttgctatttc ataatcagaa ttcaggatca gttcttcctt
     2701 agcattttta aacaaccgag ttaaaacgaa ctgatataac aaactataaa aaactagcaa
     2761 atagtcaatt tgtcggtact cggtattctg atcaattgca tatagagaga ttaaaacgaa
     2821 atacacaata tcattcagct cgctaccctt gatctcaaaa ttattgacta agtaactatc
     2881 aatacccatc aagtctgcaa aatctccata caaagaaaaa tatgattttg acaatatatc
     2941 tatatctgaa tcgtcaaacc catttactat tttcccatca ttgttagtag ttgctaacga
     3001 agcaatagtg acttttgaat tgggctttac aaatcctata cctaatagtt ctttcatctt
     3061 tttaatttca agtttgttat ccttttgttc ggctttatgc tgtttcatat tgtaaagcgg
     3121 ataaaatgct gttccgtaag caccttttag tacgttatct ataaaataat cttcagaacc
     3181 tgttttgaat agaaagttaa tcgcagccaa attagataac agctcttcag tgttgtgcat
     3241 caatttttta tcatttaata aattatcccc aagatatttc attgcataat aagggtttgt
     3301 ttcgctactt atttttaata ttttttgata gctattcctc acgatattaa ccaaaatatc
     3361 tgtctgatta attgaattac agtacaaagc cactcctaat gccatataaa cattcaaatc
     3421 atgttttgcc gtaccgttga ataaaacgtc ttcaactttt gaattatctc gtctatactt
     3481 tttgatcata tttttgatat tttgatcatt ctcttggtat atttttttca ctcgttccaa
     3541 tgccttgggt ttgttggaaa cagcaacagc gaagtctagt tctttgaaat caatatttag
     3601 tatctgtacc tcatcaccta gattataatc tctatttacg ctcggaaaac tgctgaaatc
     3661 taactgtttt ttacctattt ttggcgaaaa tattccttcg atttctggca aatgtaatca
     3721 cactctctcc ttatttatct ttagaaaatt tgtcactatt ctccttatat ttatcaataa
     3781 acctttttaa tcgagcaata taagcgttaa cccttttagg ttcttctttt cgccattcat
     3841 cgtttacgtg accattttca aagaaagaag ctaaaagagt ttccacttca ttaaccatat
     3901 ctgcgatcgt atatttttct gtaccctcac tcaattcttc atgaactgtt aagtgttcat
     3961 agctaagttc attcataatt ttgttatagc gttttcttgc tttttcaaat tctttctgtt
     4021 cgtcaaattc cacttgattc ttcatgttgt tcttcctctc tgtttgatat tttttcactt
     4081 cttcaagttt agcatctagt tccgctttaa cttctgtcac ttctttaagc gcttgggata
     4141 acttatgaga caatttcgca taagtttctc tcttgtattc atttcctact cccaactctt
     4201 cggagtagaa ctcttctagt agtcttgcaa aattaacatt tctcctgcca gcatagtttt
     4261 ttagcttctc aaaatcagat tgaaagacag caacactttt gtaatccttt tcatcctcaa
     4321 aatcaaaagc atagtcatac tttcctgaaa tcaattcatc aataactgta gcaatatatt
     4381 gagagataga aatattatct tcacttctat gtcgctctat aagattatat atatcagggc
     4441 tgacatgaaa cgtacttctc ttttttgttt gcctttttct aactgttacg accacttttg
     4501 gaacttcact atctccattg atctctttgt atttttcaat aaataatttt ctagcttgtt
     4561 ctcgactttt tgccggaatt tcatctacaa ttttatcttc taaataaata tagtgtgttc
     4621 tcatacattc gtcctctatc ttctttactc tcaatcttca tgatcttagt tcttaccttg
     4681 tttactctta aacctttcaa aaaaatctag tcaaaatcaa acttgaaatc atcgtctatc
     4741 acaacaccct taacttcatc tttagagttc ccaaaggaac ccttattttc aaatccttta
     4801 tttttgaatg atgatggtta tggaaaacat cctttgaaat tctgacaagc ataactagtc
     4861 agaactgttt tacttagatt aagctttcaa aagatactga tgaagaacta agttctcagt
     4921 accttcccat tcttcaataa aaaatatttc tccttctttt acccactcta tagctaattt
     4981 cttataatca aataaaccat atgtgtttaa gcctttgtct ttcattaaac tcaaaaactg
     5041 atcccctttt aatctttctg tatcatccat cagaaattca actaaatctt tatccatgcg
     5101 tgcaactttt tcgtctttta gtgccattga tttagcatag tcagttgaat cttttagaat
     5161 aataacacct acatttcctt ccacaatttt cttcttcata tttacctcct accatctttt
     5221 ccaaagagta tttagtcaaa atcaaactcg aaatcatcgt ctatcacaac acccttaact
     5281 tcatctttag agttcccaaa ggagccctta ttttcaaatc ctttgttttt aaattctaaa
     5341 gctttttcga cttcttcttt ggatattgta tgaacttttt tcttaactac tccaagttta
     5401 tacagctctt caaactctgg tacttcggat ctgaaataca atccatcagt ccgaattttt
     5461 aatagcccat atgcgctatg aacgtatttc tgttcatatc taaccatacg atctctaaat
     5521 acgcctgcta ccatggagga atacgctcga ttatacatac tgtatttatc gaaggtgctt
     5581 gtttctcctt ctcttaatga tttttgtccg atagcaccaa atactttcgt tctaagattt
     5641 ttataaagct tcaacgtatc aggatcagtt tttaatggct cgattctttc aaaatattct
     5701 ttgagtaatc cttttttcgt caaaaatgtg tggctatcta ggacttccat ctctttaata
     5761 tcataataat caaggataag tgttagctca atctccgata accaaatagg ctcattagct
     5821 gttttgggat tttcgccata atccgcaaac atcgactgtg attttggatt agcatatgtt
     5881 ccttctttgg ctgttccttc aaaattaacc aaaaagaaca tatacgtttt taaattttca
     5941 tacggtctta cttttggtct atttatcgct ctcaaaatag cttgatctgt attatatttt
     6001 gctttcaaat aagtgtttga cggcaaagga taatttctca tgatcctcga ataggcttcg
     6061 ttgaaatcat attcgatcat ctttgctcct tcctcaaaca aatccaagtt atacatgcta
     6121 ccggaggtac tggctctata ggctgaactt ggaacaaaga aaggtgcatc ttccggctta
     6181 tcataaccca aaaaatgtat tcctgtttgc ttgttctgac ggtaaatgtc catcaaaaga
     6241 tatttgatcg tactaaaaga atctaatttt cctcttgtca tccaccctaa tccgacaagt
     6301 cttttaattt tattctgcat ttcaggtgtg tatatgctat tgtttcccat ttatctgctc
     6361 atctccggct tttttattct agaccctata tttgttcatt acaccactta tctcgtctac
     6421 aaaggctccc agatcgtgtg taaaggcatc gtagtaatct tcaatctcac tcgattcagt
     6481 tctccggttt cttccgtttt tatttctgaa tagatacatc tgcgtttctc ttaaatccct
     6541 tgcttttttc gcaagtgcta ataaatccgt aaaattatca ataaccataa tgtcattgat
     6601 cataaaacct tcttcatgac catacttttc agccatctcg ataaaatcat taacatcgaa
     6661 caactccaat aattctgctt tatatttctc taacaatgta tttaaatagc gatttctcaa
     6721 ttcccatgag ctttcaccag ttccgtattc aaccttgatc cctaaattct tagtgtagcc
     6781 ttttgctgtg tatcgtccaa tgaagtctcc cttattccga ttgctatctt cttccttgaa
     6841 aaactcacgc caaattccct ttttcttttt aggaatcaga cctctgctct tgaaatcatt
     6901 atattttttt gaagcttccc ttcgtgtatc taagtacctt tgcttcagct ctgtagctga
     6961 ttcattcgtt ccatattcaa cagtgatcct taatcgtttg ctatagcctt ttgctgtata
     7021 tcgtccaaca aagtcaccgc cacttcgatc actactgact tccttaacaa attctctcca
     7081 tatacctttt ttgtctttcg gtattaatcc tcgtcttttc atatcttgat atttttcaga
     7141 agcttctttt agagtattct tttttgctac catgtcatca cctcgcaatt tagttaactt
     7201 tatacacgtc tacaaaaaaa tatgattact tactttttaa attgaaaaac tcgttgaaat
     7261 gctcttcttt caacacaaca aaagcatgat aacttaacct taaaatataa tctccatatt
     7321 caactggata atctttggtt ataactctaa ttaccgctct tccgctattt acaagttcac
     7381 tttttttctt cccccttcgt actcttcgta tactatgagg ttttacaaat tcgactactt
     7441 gcttccaatt atcattttta tattgaatac acagtggtga ttttttcatt tctatataca
     7501 tgttcttata atcttctact ttcttatttt ctttaactag atgtttatta ttctcataag
     7561 acaaattttc gcttatcatt taatatttcc cttccttttt ttgcattata tttagcaaac
     7621 attcaccatt tgaatttgac atagctttaa gacagatatt ttatcttttc aaacattaag
     7681 ttatttgtct ttttcagaag tggtatagga tcgaagtcct tcacgtttca ttatgtcttc
     7741 tgcttttcca atgctgatcc ctttccaatc acgccatcct acgcttccct tattttgacg
     7801 ataggcttta atagctgatt cggatatttg ccatcttgta gccagctctt ttgcggtgta
     7861 cttatttgag aaaagtaact tctctatcag tgaaatatca atcattttat tctcactcct
     7921 gaaatcttca ttaatcatct ctagctagct atatcttcaa tcaattctaa tatgtcggat
     7981 aaaccatcaa cagcttcttc aagttgatca acggattctt ctgctttttc atatctttct
     8041 gatccctgta gatttttagg tatattttct ttgtactctt cttcttgaat tacttctaac
     8101 tcatccttta gctcggaaag tcgctcgctt atttcttcta acctttttct tcttctttgg
     8161 ttcatcaaaa tcacctcttc gcataatctc tacgatttta atataccact tttgtgtact
     8221 acattcaata ttttttgtta tagattcgta ttacaaaaat ttcctctaaa aaaataagag
     8281 cctatttacc atttcagtaa ttaagctctt atttcattta gttgataatt ctatcaatta
     8341 acgtacagca accgccattc cagtcacatt aaatgctctc tcatacgtgc ttgcattgat
     8401 ccattttgcg ccaaatacag ggtcaacaac atggtatgca ccatttctga agccatccac
     8461 gagaactaca tgtccattcc atactgcacg accccaaggc atttgtttga actctggcgt
     8521 agcatagtta taagtaatcc aagttacaac cggattacca ttgcgaattt cattcttgat
     8581 tccattcgtt cctgaaccag tgatattgac ggcattaccg ccaacgtttt ttgcccattt
     8641 aactactggg tcagccatca tgccttgata cgttccatta acgttatgac gccactctcc
     8701 tgagtaacca tgataaggat tattgtcatt agcttttggc atatagttta caaaactggt
     8761 taagctttga tttgtggcta cacctttgta gtgtaaacct tctaataaag aagctccttc
     8821 gcataacatt gtatttcctt gccatacaac aggaacgttc aacacttttt cagtgcttgg
     8881 agcaggtgta ggatttggta cactgtttgg cattgcatac cagcctgtac cttcaatctt
     8941 ccaaccttgt ttactaatta cgttcttttc attttgatct aacgtgtagt gatgccaatt
     9001 cacgtatttg ttatataaac gtgttacagg aactctattt gctccgttgt aggaatagaa
     9061 cgcaacacct tcgtacgtcc atcctgtgtg tgaaatctta tctctttctc cggcatcaac
     9121 tgtgtaaaaa tgttcaccgt tataccgatt atacaaacga taaacaggag taccacttgt
     9181 tggagcttgc catgcgacac cttcgtaatg ccatccataa cgtttcagat tgtctctttc
     9241 tccaacgctt ctagtgtata gatgctctcc gctattggga ttatacatac gatacatgga
     9301 aatagagctt tctgctgaaa ctgaaggtga taataatact agccccattg tgaatagaat
     9361 tcctactaaa aattttttca tttgatttta ccccttttca ttttttattg tcattattgc
     9421 atgaaaatta ttcgtaagat tgccaattac ataaacgatt gttttaagat cataatcgtt
     9481 gttgtcttaa attcattcct cctttccaat tcaagagtac ctatcaataa tgacacatac
     9541 tcttgaatct ttcgccaaat cataaacttg aatatcttgg taagaattca tcacccatac
     9601 gtttgttttc tctatctata tggttctgta tacctacaac atcatgagcg aaattaacat
     9661 catcagaatt caacaaggct tttaatcgca gattaaattc ttggtgggca acgcttaaat
     9721 ccatctttaa agaaatacga tcaaaaaaca taattcccat attatttgct cgttcaacaa
     9781 tttgaaatat atctattagt tcttcttttt tcataactgc tcttccttcc tttaacttgt
     9841 taatctaact tgcttacaaa tatatcctac cacatttgtg tactatccgt caatattttt
     9901 ttgtgtataa atgtgtatta taaaacaaat aattttacac aaacaaaaaa gagcctaact
     9961 gctcttagca ataagctctc atcactgctc ctcgtgaatt tagtcagtac aatgtatttg
    10021 ccttttcttt gataaaatcc ttatacgaaa aattgttcaa ccaaaatttt tttagcacta
    10081 ccgaattttt gtttccatca ttcaactcca attgaccaaa tctgctgatc atatcatcaa
    10141 tttcatcaat tatctcttgt gccatcatgc cgtcttctag acgttctaat acgtacaatt
    10201 caaaaacatc ccttttcatt caaaatgccc ctttcaggtt accttttgcc attttatggg
    10261 ctaactccgc ctactcagct ttttttatat acctactgat atttgctttc gtatttcttg
    10321 gcataccacc tgcttgcagg taatgttcac gataataagc atctagctct gaaacagctt
    10381 tttctaatct attcgttgca ttcaacactg agcggtattc acgtttttcc cttaatacac
    10441 gtcttaaatg gttcatcgct gatatataag gcttatagta ttccaataca gtatatcctc
    10501 tagaaatata ggcaattgga tttctagctt catggatcaa ttctaaaata tctgttggat
    10561 caggcttatc cggcatctct tcttcgattt ccataatact gatcaagtag tccaatccgg
    10621 aatcatcgac tccataactt tcacaccatt cttttaggtt gttcaagtca tgaataacat
    10681 cttcaggatc aaactctcta aaagcagtga tcccttggct ataatatttt atatattcca
    10741 ttgccgattc aaaatcctct ggcggtatga aagcttcaat ttcttcttca tcaatcgctt
    10801 tcagcactat tccgatacct gagtttgaag gatattttct caatccttct tcaattaaat
    10861 cctcatctat tcgtcctttt tgaaacatca ccaataattg ttctaacagc tgatctaaaa
    10921 ccctgcgctc ttcattaagt tccattgcat ttttaacttc tttttcaccg agttcaaagt
    10981 aagctttgat caacttttct tgttcttttc gttcaactaa tttcttcttc aagtcattca
    11041 tattgagaat gaaatccatg tgattactct tttgctgatt gacaaaatca tatctttgtt
    11101 ccaaaacatc ccgatatttc tttagttctt cttgaattat ttcaaagcta tcaagtacat
    11161 gaagaatttt ttcattatta ttcatcagcc catctcctgt agtctcacgt ttgttttcga
    11221 cttttcaaaa gtattgtatt taaaagcaat taaccctaca accctgctat ctttgatgcc
    11281 tatttccttg ttaaaaattc cgtccaatgt tcctttgact aaattatcaa tatccggctg
    11341 tgaagtacgc atcagtgaat caagcttttt cttatctttt ctcacttggt aaaaataatc
    11401 tatctgagct ataaccccca taggagcatt cattaccttt ttcagttgat catttaccaa
    11461 atctttctct ttaagcgtat tcttgatatt ttccaaatag tcgttgtact cttttggata
    11521 gtaagtgata atcccatttc ctctaaattt agactttggt cttggcgttg ctactggttt
    11581 aaaatcaaat gtagcttccc atatatctcc gttcaatcct tcagaagtat cccactcaaa
    11641 ttctgaatat cgtttgatcc tcacaactgt ttttccttcg ttcgccacat tatatttaaa
    11701 agcaattaag cctgtgatat ggctatctcg tatagctgag atgttaaaaa taccatccat
    11761 agcacttttt actaaattat ctaaatctgg cgctgttgtc ttcataatgt tatagacttt
    11821 tttatagctt tttggtatct ggtagtaaaa gtccacttcc ataattgcgc ccataggatc
    11881 atttacaatc gagtaaaatt catcatctaa taaatcttga tcttcgataa agctctttat
    11941 ttttttcaaa taatcaagat actttttatc atagtaggtt gaaatccttt tctctgctac
    12001 gtcattagtt tcatcagcag cattccttct tgcacttggt cttggtgcag atacaggttg
    12061 gaacggtaaa gttacagtcc acattagtct tttttgatct ctttcaaata gattttttta
    12121 aaggtatcaa ggcttaaagg agctaccttt tgcatcttcc ggattttatt tacttgttcc
    12181 aattgtttca atagaccatc ttcttttcta aggtactcag gatttgttat ttgtttattc
    12241 cgatactcga ttaatgattg aagctctctt actgttaaaa catcctcaat ttttgcttct
    12301 ttactcgtta tcttttttag ataccctaat actgttggat aagatttgcc gatcacttct
    12361 gcgatttctt ttttattgta ctcaccaaat tttccaagca accagtcatt catgatctta
    12421 cttgatactt ttgggtatac aagaattgct ttttcaatgg aatgactgcc attatttctg
    12481 atcagttgac tatctttaca ctcaactgta acgccatcac ctaaatcaac aacatattgc
    12541 caaacgcctc ttcgattctt attaagagaa ataatcgttc cctctttatc tctataatcc
    12601 tttgataatt cacttactgt tgtaactctt tccccaatgc taaaatgtct aatatcatta
    12661 ttgttttcca cttattatcc ctccaatatt gctggttatc tcttcctctg acaaggaact
    12721 atatatcgta taaagaattt taacattatt cggaatgagt gtaaagaaac tttatataaa
    12781 aagagtgatt tagtctaaga ccaaatcact ctttcttttt ttattaatta tattgttatc
    12841 tgatagacct attagtaaat gcacgtctta ttgtttgatc attctcgatc aattcttcta
    12901 acaactctga aactgttact ttcttacctc ttgctctttc ttcttcttta gctcgctttt
    12961 ctaaatattt aacagaagct atttttaaag tcacatactt tctaactttt tctttattta
    13021 ctaccatcag ctatcatctc caaggtgtca cacctatata ttattggtgt gacaccattt
    13081 gtttattata actttctggc tatttagcca atttaatcgt taatgcttcg tttgctgtag
    13141 aatctccaaa aactttaccg tcttcataaa ctacatgaac tggcaaatct gcaccgtctg
    13201 aatctggttt agcagtacca cgatacaaac caattactct ttttcctagt tctcttgcaa
    13261 ccgtaacttt tttatcctga tccatagatt caactgaatt gaataaggta acattaatca
    13321 tttgcttttc agtatccaat ttaacagttt tgatagcttt gaagacttca gtgttaaacc
    13381 gatcagccat agaaccagaa acagattcaa acgatttgac tttttcagga tcaaaatcaa
    13441 ttttatctgg tagttccgct tcgacagtag aacttgattc agttgtactt tcttctgtag
    13501 ctacttcttt tttcgctgat tcgataatct ttttcgttga tgttttattt atttttacat
    13561 ctttatagta ttcttctcca tcaacgttaa aaaccaatgt cagcttgtgt acagggctat
    13621 tctctttaac tgtataaaac acttcaaatt gtgattgaga cggcttaata gtagcgagaa
    13681 cgtcgtctga tgctttcttg ccatcttcct tcactgtcag cgaaacattt ttataactcg
    13741 ttacattttc aaatgttcca tctattacta cccttcccaa agaatctgga gcataactat
    13801 tattaactcc cgatatacca aattgtttat cgttacttcc aaaaatctta gtggcattaa
    13861 acacaataaa ggttacaatc acaattccaa tgcctatgtt acgcataatc ggattatttg
    13921 tttgaaattg accaataatt atagcgattg ccccaaaaaa agcaagaatt aaacataaat
    13981 aacctaacag ggttaatagc atgattcatc acaactttct attctttatt tttctttttg
    14041 gttttcgttt gttcaaactc atgctcattt agataagtaa tcaataaatc tcgcatggat
    14101 ttgctatcct tacttagcgt atcaaacgct tcttgtaagt cagcatcctt ctctacagca
    14161 ttaaaccaat gcttcgttcc aaattcaaaa agattactaa tcactcttaa cggttcttga
    14221 tcagtattcg tattaagcca ttcaagtatc cgttgttgag ctaatcctaa tttactttcg
    14281 tcttcgtgat ttgtacgtgc ttcatagatt ttagtgtgta tatcatcaaa atagtggatt
    14341 cgttctctta gcgcttcacg ttgttcgtgc atagcccttt tatttctaac ttcttcgtct
    14401 attgtttcaa tcttttttct taacagtatt tgaagtgata acatttctga gacagacaaa
    14461 aacactggaa ttttcttttc ttttcttttt ggcattttta gcactccctt ttaattcttt
    14521 tgtttgatca atgaaacgtt ctttagaata aatcccaaga tcattccaca gacaaatccg
    14581 attacatgtg acaagacatt tgcctcgcct gattcaaaag tagataccaa taaaatgccg
    14641 attatcaata atacaaaaac acttagtaat aaatgttcct tgctatcaaa atggatgtat
    14701 gcaatcgcaa ataaagcaat ccatccaaaa atacctccac taagccccat acttacgatg
    14761 ttgctttgta gcagaaaagg aactaagctt cctagaaatc ctgatatgat taaaacgatc
    14821 aataatgtta accactcata ctgatcattc agtgcttctt caagaactac taagaacaat
    14881 aaattagaaa ttaaatggta caagttaata tgagaaacga tagaaatgaa tatattcggg
    14941 aacaaatgtc cttcatacaa accacctaaa gcaaccattt cctttgcact caatgtattt
    15001 ccaattatac tttttccaat ctgtaaaaaa tcaaacagga aaatcaatac acaaacaaaa
    15061 actacagtcg ctactaagta tcgactacta tttttgtttt tttctctcat tcgctacttc
    15121 ctttgcttta gtaactgccg attctccata ttctctaaaa agagtcgacg ttagctttcc
    15181 tctcggaata ttgtattttt ttaaaatttc agttgttgaa agattttttt caaagtcttc
    15241 aataatttgt ttatgaagat ttattttagc caaatcattg gttgtcaaaa caggttcttt
    15301 agcttgaata tcgccttgac gaattaacct ttgaatttta tttttgcttt ttaaaaaagg
    15361 atatttttca attaaagggt caattgcatt cccttcatca atccacttcc ctatttcttg
    15421 ataaactctc ttttgaaatt ttttcctcag ctcaatcggg ttcggaaaaa cattgtgctt
    15481 aaagtcgtgt gatactaggc tttttgattt gcctaattca tcagcgacct cttgcatcgt
    15541 tattttgttt tcgatatagc gttggacaag agcattatat tttttgaggt tattatcatt
    15601 tattgtcatg ttcttaccta cgttcattgt taattttttg gtatttttct gtttgtgtat
    15661 tgtgattttt aaaaggtatt tcctaaaacg ttaaattttg ataatcggtt aaattttact
    15721 attttatcaa tttctgcctg tgaccacggc tctattgaac acatttggta cgtcactagc
    15781 atatcaacat attcagatta tctagtggta tatattacta tacaaacaaa atcgtgatag
    15841 ctacaatctc gtccttctta taagctgacc aaccttcact atcctcaacg attaactccc
    15901 catctaaaga catattttgc catctatcaa ttatttcttg actactaagt ttcagcgaat
    15961 ctatttcatg aaaaaatgac tctccactag ataaataaat actcattatt tcttttttgt
    16021 ctatttcttc ctgctctttg atcgcttgtt cagttacttc ctcttctggg tctacttcta
    16081 gacctgattt aacagtctct tgattatggt tagatattat ttgttttttt attgttgaaa
    16141 aattttcaca agttaatata actaacgcaa ctgtcagaac gaataaaatc caactcatta
    16201 tttgtagtcc tgcctttaaa tcttctagat aaccaatttc tcctagataa acaatcgcgt
    16261 tttttaactg atttattatc cgacaaatag taaacccaga tactgctatg caaagataca
    16321 ttcctgcaat aagccacagt attcttcttt tattcattta gcttatcctc cgttgaaaag
    16381 acactttaag agtatgtgta ccccatcgca tattcggatc ataatttttc atttgctgta
    16441 tcctcctcat aatcagttaa tactatatat gaatagatat aatattcctt actcatcatt
    16501 ccggtatctc tactatctcc caaaatggtt ttactttata taatttctta acttcacgat
    16561 tcatcgatct aaccatgctt tctaatacag ttgatcttgt acctagcgaa actttttgtt
    16621 tccttgcttt cttaacactt atcatgctag gatatttttc attaaacacg tatacgcctt
    16681 gtttataata tttacgcatg agtgtgtcgt cttgtagcat tttctcccac aaatcactag
    16741 taacgactaa atagttataa tcgccgataa atgtctgttt cgccttgctt ttcaaatcgg
    16801 ataaagtaac tttgatctca taacatcgaa ctgtgttatc tactgaataa gttatataat
    16861 ctactctttc tctgccatcc caaccgatag ttacttcaaa acaaccaaat acacccatgc
    16921 gatttgtttc atgccataaa cacttctcag cttgtctagt aaggtctgtt ttcacttttt
    16981 ttcaccttct aaaactttga atgtactttt tcgttcatct tttaatgctt cattaatttc
    17041 ttctttactc cacggaacga tcaatcgaac agaatattcg acaaccaaat caacaaattc
    17101 atagcgaaaa ctattgatag acactacttc atatgctatc cctttctcag gaaagacaat
    17161 atctcctatt tttattattc gcttatcatc ttttgattgg tcgtatcgat tatccacaat
    17221 aacttctctt ttgcagttat aataatcact caagtctccg actttctgaa ctagaactct
    17281 aactgttaaa ctaatagatt tgctaaaata atgtttgtta attttaagaa tgcgaataat
    17341 aatatactta tcacctctat attcaatgga ttcaccaata cttacttttt cgttaaatcc
    17401 ctctctatat agttttagaa tagttaacac aattttcacc tctactgcca aacaaattta
    17461 gctttatcat gccatgtttc agacattact tttttaccat tttcgtcaaa tatcaccgct
    17521 tcccactttg ctgttccgtc cggctgttta caaatatctg caaaccatag gattgttttt
    17581 cgttcgttga attttcttac tttcttctca aatttattga tctgaactct attttgattg
    17641 ctgttccaac ctactaatag gcgattatct gattctgtac catgaaaacc gatgggaacg
    17701 atgtgggttc tatcaaatgc ttcttttcct tctggataga gcattggtct aagtttccta
    17761 cgtttcaagt catctcttgc ttgataccgc catgccgatt ttggattgaa tttaatagct
    17821 atttgatgct tgcttttgtc atgataaaaa tatgccacat ctgcttcagc gccaagcaac
    17881 ttttctcttg cactatttga ttcaaacggt ttattcactt ctaaaacctt cacacccaaa
    17941 attccatgat caagcatctg tttaggagtt aacaccgctt ttttatcttt tttcttagct
    18001 cgtttcacaa ctacttcatc tgtatattta tatggcgtaa gccacctcaa aaaagaactg
    18061 acaatgatca tttatgcacc tgttttttgg cttttaacat tttttgcatg ataggtgaag
    18121 tagttccgtt tacttgtttc ttctcagaat tatcggttgt gatggtcact tttactgaaa
    18181 ctttcggatc agtagttttc ggtttagttt tagatgaaat cggctgttca ttattcgtgt
    18241 tgtttgaggt ttgcggttca ggatcaactt tatctaatgc ttttaaaact ttttctaatt
    18301 tttcggcttg aagtttttct agtgttttag ctccttgttt ttctttttct tctttgtttt
    18361 tttcaagttt tagagcaaac gtactcttct tttcaattga tggctcttta gatgatgaat
    18421 ttgctaaagg tgtgttcaaa tttcttttag cagattttct tacaggagct ttaccaattc
    18481 caccaaaatc ctcgtcttca ccgccctcac cacgagattc agctacgatt gctcggtaat
    18541 caacatattc tggttctggc atagtatttc taaggtgatc aaagatcgct tcaatttcct
    18601 cgttgctgat ataggctcct tgcgctctga ttttgtttcc tccattcaac ttgataaaca
    18661 tatctccctt tggacttagg ctttctccac caccctcatc taatacgatc ctactttcta
    18721 aatcacttgc taccatcaag cagactctag atgggaaatt tgctttaata atacctgtca
    18781 cgactgtaga tcgaggtgat tgtgttgcaa gaataacgtg aattcctgcc gcccttgcca
    18841 tttgtcctaa ttgaacgatc agcccttcaa cttctttgtg tgttccaact aattgcgaat
    18901 attcatcaac caataagatc acatagggaa atttctctat tccatgtttt tcagcgtatt
    18961 tgttatattg tgtgatattt tgaacgcctg ctttttccat caatttgtag cgttcattca
    19021 ttaagatcac aaggtattca aaaaattcat aagcttcatc catatcagta attggatcag
    19081 caagcatata gggtaatttt ttataacgcc caaactctac tcgttttggg tctactatcc
    19141 ctagctttaa ttcatcaggc attgcatgac agatagctgt aacaagaatt tcattcagta
    19201 ataccgattt ccctgatcct gttgttccgg caactaacat gtgtggcgta tcgctgatgc
    19261 aaaaatgagc aattgtgcca tcaggcttaa ttccaaaaat agcatctgtc gggtctttag
    19321 cctttgtgtt caacatattg acactgaatt ttccattaag ctttatatta ggttctttat
    19381 ctagcaaaat agattcagct ttttcggctc tatttttgac tttctctaac aatttcatag
    19441 ccatttctat tcctctcttt tctcggtact tttctgtttg tgaactggta gtttgtattt
    19501 tctgtttcta tttgtgatgt cttctaaaac acgctattga tcattgatgg tacatcaatt
    19561 ggaatacgat cattttctcc taatggtaaa gtaatttgaa tacgccctgc gttgatccgc
    19621 acgattacac ctgatatatg caagtaagtt tcaatttctt ctttcattga gcttagttga
    19681 atttttctgg tacgagataa agtaaacaaa tattcaatag atgtattccc aacgtgcata
    19741 tctgttagac ttgcaggtac gtcagcagaa gatagataaa gttctaaaga ttgcttttga
    19801 gtttcggcat attttctagc tttttcggtt tggttttgga tatttttgga gttatctgca
    19861 aagagattga ttggaaaagc aaattgtgaa tttgaaactg caatggatgc tgttgatttt
    19921 tccgtttcct tgtttttggt acttactctt tccttacgtt ctctttcaat gtttactgat
    19981 gctctgaaga tgaagtaatc acctgaaact tcgtcttctt tttccccaaa tttagctcgc
    20041 cctcttgttg ctcttgtcag gatcgaccct aaatcttgaa tttgtcgtaa aactgtttct
    20101 ctaactttct tcccttttgg ttgcggtatt tttacgatat attctcgaat atcttcattg
    20161 acacttagat cacgtcttgt atgaacagaa attttgatat ttttaagcgt tgatagcaca
    20221 tcggattcta attgcgtttt gtttcgtttt ctttttttgt tgttcgtttc atctcctata
    20281 atcttgtttg gatcatactt cacatccata gcttctagag cttctctttt caaccttcta
    20341 ctttccatat catcaaagaa tggtctttgt tctccatagt gatgcctgaa catcaaagta
    20401 accaaatatc cgacaaagaa taccgttcct gcttctaata ctaaaataga gatatctacc
    20461 aagtgtagct ttgcaaaaaa gctttgtaca ttatatgaat aacttaaata atttggaaaa
    20521 aacgcattga gtaactcttt aaacatctct tttaatccta aatcatctgt tccgttaaca
    20581 tcatttaacg agacgagaat acttactaag gtatctacaa gcagttgtac aacctttgtt
    20641 acccaatgca aaatatatat gccaattgca ctaactacca atgcacgtcc aaccagatat
    20701 actgatttag aaaaattggc tacaggataa tatttttctc catcgcttcg cttatattcc
    20761 tttaattcaa tggatttttc gctcatctga atatctcctt attttctttc tgattttggt
    20821 taatcgtcac ttatgctgtc ctcacacatc tgcgtatctt taggaatgat tactctgatt
    20881 tcgtctaaca tttctctctc aatgatcatc gccccttttg tactaacagt tttctctgat
    20941 aattcaacac atttccctat ttcagaatct ggatcaaaaa tagcctgttc tacgtcaaaa
    21001 tgattcttag ctacatacac gatactcatt gtttctaact ctctagcaac tgaatattta
    21061 cccttcttca gaagatagta gccatttcct ttttttattc gatttgcaac tgatttatct
    21121 aatccattga tgcttattgt ttccaattct tcaccttctc aatttctaat ctcttaccca
    21181 agtaccattt tgatagttga tgttgattcc tttttgattg ttaaatacat aaacattaag
    21241 gtttaccgtt tttccattat cgttaactga aaaagcttcg acatgagaac cacgaggaac
    21301 caattcattc cctttataaa ttggatcaac tttatacaat actttttcat tcttattttt
    21361 atagattgca tcacgtacaa tcgtttctga gtaagccatc cctccattct gatcattttt
    21421 cccaacattt tgcgctctcg ttccaagtgt taaattgtgt acctccatat ctcctcctag
    21481 cgaccatgct aatgaatgac ttttgttata gagataacct ttatagcctt ctagctgagt
    21541 gatttcattg tttgaatctt tgccgaccca agtatctctc tcttgatcaa atactccatc
    21601 cttgtattct cctgcaatat ggacagaact tttgaaagat ggacgttcca catttttaga
    21661 tgagtgagaa tttacagcat cataggtgat caacgcttca acatttcctg ctcttccaag
    21721 ttgatctaaa tctgcataat ttacccaaaa ggtttgactg cctttttctg ataatgcttc
    21781 atggtcttct tttgtcagtg tatatttccc atcattaatg tttacgatat tatttggata
    21841 tttgtttgga tcataacgta tccctaaaaa ttcttttgat tcactgtctg ctttattacc
    21901 tacctgaaca ctacttttga tctgaaaatc atgttcctga gtgctgttat tctgaaagta
    21961 acccttgcca ctaagaccaa ccgcaactaa aaccaaaatt cctaacaacg atcgactaaa
    22021 cttcttcttt ttcataccat cattcctttt attgtgttat cataataaga taaatatata
    22081 ataaactata ttgtaaattt tgtcaaatgt atttttattt gttcccacta taaaccttga
    22141 tatatagcca atttaaaaaa ggatgacaaa aaattatcaa gttaaaaaaa taaattttga
    22201 accaaattat ttttaataaa atagctagtt tttttatcaa aaaaatagag cttttcagct
    22261 ctattttatt caatatcatt ttgatcaatt aataatcgta cactccaatt aaccgagttt
    22321 tgatcactca aatatttata atctttgtca tcaaaaacca gttgccaaac tctggtacga
    22381 acagtttctt cgtccacttc ctcatctgaa gagtattgcg ataatgcttc ttcaactata
    22441 tactttacaa cttcctcaaa atcagtagga actttatcaa taaccacttc atagtcttct
    22501 tgttttacga tattgggatc aatatgttca tccaaacata cctcagtgag tttttctttc
    22561 acctctgtat ctctttctgt ttttaggtac tctgcatagt cttccttaga ccattgtttt
    22621 tcaagatcat ccgctatact tttaataaag gattctttat ttaaccaatt ttctgcggta
    22681 tctatacctt caaactcaaa agctgttcct tctatacgtg atttgacgaa agcatcaatt
    22741 ttcttgtatt gattattcat ttcccatgaa acacactcat cttcttgcgt aatatcataa
    22801 attttttcgg aaaaactaac taattcattc cacgatttat cctcggcaaa ttgacttagt
    22861 ttcgaagcga taaaaggaaa ttttgctttt aagtcatcca tattcttgat ctcaaattcg
    22921 atattttctt ctcctacagc tgaaatccat gctgaaagat tgattgcaat atcgctaaag
    22981 tcttctacat tttcgacatt tcctgtatta tcttttatta ctgcttctaa agccatatcg
    23041 gttgttgcac tcatggtttg atctcctctc ataatagagc aaaaagccgg cttaaccgac
    23101 tttttgcatg gtacgttttg tttggtagtt ttctgcttgt gtttatgttg aaataatccc
    23161 tatagctctt cttttaatag ttcttcaact ttatctctta actctccctg accactctct
    23221 aatagattta aatagctcat cagaatatcc gtttcatacg tttttaattc agctctatac
    23281 tcactttctg aatatgatcg taaataccca tcattgctaa atgcaactag atcatcttga
    23341 aagttccatt ctcctaaatt tgttttactc actgcttccc atggactatt actaaacatt
    23401 tcattaaaga aatcttcgtc attttgatat accttcaatt caggaaaata gttgctgtct
    23461 tcattgattt cttgcaccat ttttccaagt tcttcttcag ataatttacc ttccaataat
    23521 tttacttttt ctctgtcatt tgcataagcc atttgaacct ctcctttttc tttttcaatt
    23581 tcttcaggtg tttgttttgg cgtaactttc aaatctattc cattgcctat tagacctatt
    23641 agataaccat agaacgtttt aaagtcactt gtagcaagag atttggcatc agggtatgta
    23701 tcccctccaa ttttatctaa aacaaaatca gcaatcgtta cccaaggaga atatatcgta
    23761 cacactacat cccatgtagc atcttcaata ttcgtccatg tcggaaagtc attttcattc
    23821 aaatagtctt ttttcattgt ttcgatcaga gtttttccac tttcgttttg gcttagatat
    23881 tcaacaaatt ctgcactatc aaacctgcta ctaaatagtt tagctaatga gtctaaagct
    23941 gtttttgata ttcccaccat tactacacct cctattaata ttttacgatg acttgttttt
    24001 cattcacaaa taagattttc tacatcttat ctatttcttt atagcttttc catacattat
    24061 ttctaaatac aaccaataac cctattaaat taagcttttg agtttatctt ttctaataat
    24121 attctgataa atattctgat aaaaaaattt gttgtatttt tttcttgact ttttctttag
    24181 aattaagatc agttttgatt ttatttaaaa gtgaatctag ctctattcaa aataaaaaag
    24241 agatcaataa tagtatcaat ccctttttac tgtactctat caatttttat aatcttcatt
    24301 atgcttttcg aaactctttg attaaaagaa taagaacatt tagacttgct aatgctccta
    24361 ttacagtcca aatgactaaa agcattttct gatgtttatt ggtaattcct gtgaatattg
    24421 aacttgcctt ttcagcaaat gttctgtctt gcatctctac gctaccttgt tccggaatat
    24481 taagatcatt atttccatct aattccttgt tggcacgatc aacatcctca aataaatcat
    24541 tagaagatgt aacagtttct gtcttatctg aaacgtactc gctatcaatg gcatcagcgt
    24601 atgcggtatt gccaactgtt acttgtggcg tgatgtattt tgctttttca tctcttagac
    24661 ttctaactaa taaacgatcg tagttctgag gatatggatg acaagtataa agtgttaaca
    24721 tatctaaatc aggaatatca gtcagtgttg aattctgacc gtaatttcta ataacgtctg
    24781 ttcggaaaac cgtataaact gctttttcac caagataatc tatataaatg tggtctccgg
    24841 ctactaattg atcaacataa agaaattgtg gtgacgtata accgccacga tgacctgcta
    24901 ttactgtttg atgaccttta cctgataaag gtagactagt agcactcatc tgtgccacac
    24961 ctctatataa atgttcctgt gaagcgccta gaaacaaaag ctctagcaca tttatttttg
    25021 gaatatagac ataaccaacc gcccctgaat aggtagatgg ttcaaaatct ttcgtttgat
    25081 tttttgaaaa aacatctcct gtgtaaggat tgacctttcc ttcagcttca ccaatatcaa
    25141 atgatccgcc gtctgttttc tgagtattgt ctaatccttg gttaccgctg ttcgatttta
    25201 tttgttcgac tttttttgcc acttcctctt ttgaaggagt ttgttttttc tcaattttct
    25261 cttggatttc cgccacattc tcttttgttt tgaattgatg atagagctga tttgcaggat
    25321 aaattgttaa aaccaaagtg caaataagag tcaaaaaaag tagtatttta ttcttcattt
    25381 ttcattcctc gtctctttgc tactattttt ttaataatga ctatcacagc tattattaat
    25441 aaaaccccta caactacgat aggataccag ttcatctgcg cctttacctt taaatcttct
    25501 ttcttctttt ctttctcaac aatacttttt tggtaaggta tgctaacccc tctaacgagt
    25561 aacctatgag aattatctcc aacaggtgta catgtcataa gtgttacata atcttttcct
    25621 gccacaggaa gcaaatcctc aaattcggtt ggtaggatta tttttatccg atctacttga
    25681 taagcaagct ttttccctga aatatcaata aaaaatttat caccaagttt gagcaactct
    25741 aaatctgtaa aatatttttt attaggaatg cccgaatgag ctgatataac agaatggttt
    25801 ccaatcccac cagttggata atcagttccg gttaatattc ctgctcctgc tgatagttga
    25861 aaatcgttta caccatcata aattggcaaa tccacattaa tctttggtat aagaatttca
    25921 cccactaatt cacctaattt atccttctga agatttccat ttgacggaat attgttatta
    25981 tcttcaggaa tcgtgactga ggtttgttct gtgtcttttc gtttcctatt aacttctgaa
    26041 tatctcttga agacttcttc attctccttt tcagaagctt catcatattt ttccttatag
    26101 gagtttagtg tttgttgagt ttggtagtag gtataagcct gagtaattac aggatataga
    26161 cctactccta aacatagcaa catcccaatt tttaagatga tcaacttgat attcttcttt
    26221 ttcatgctca tacctacttt tcacacactt tttaaaaaga aaccctagag ccaaaaacac
    26281 tctagggaat tttatttcat taagctttgt gttgttctcg tttgtagaca acatatagcc
    26341 ctgttcctgc cacacctgca accactagaa atactactgt tccaattcca cctgtacgaa
    26401 ctgtttcagg tgttttaagg ttcttgatgt ctgttacttc gctagaagca tcatcattcg
    26461 ataccacaaa tggaacatca tttctagttg gtgtatatcc tgaaggagca acaacttctt
    26521 caagagcata atcattgatt tttttatctg tgattgttgg attaccttgt ttatcaaaat
    26581 ctacatcata ggtatattgc aagccttcaa tttggaactt accgtctgat ccggaaacga
    26641 attcagtagc tttagattta tctgcaatcc aagtaacagc tttcgttgtt ggatcttgtt
    26701 ttaggtaagt aactttattc ggatcaattt tgttcttcac tacaaatttt gctccactca
    26761 atgctttgct tgtatctcca tctgttttaa tgaattggtg atcaccaatg attgtataag
    26821 cgtgatcaac tggtgtttcg tcttcgggtt catatccatt gttgtagatc gttgaaacat
    26881 cattcttaag tggtgtatcc attttagctt tactattagt atgagcgttc atcaaaattg
    26941 taaagaagcg atcccctgca tcaaattgtt tctgaagctt atccatacct gattcagtaa
    27001 agacatagtt tgtcgtttgt ccgtcacgtt cgatactgta atcagtatct aaagtcattc
    27061 cttcgatcac ttctgatgaa tcataagtta actctgcacc tagaacatct ttgacaacat
    27121 atttttcgta attaccaata ttaccaggaa ttgatggttt caatgtatat tgaacatctt
    27181 cgccagtatc aacggtttct ttatcaatta ccttagtaat tacaggtcgt ttgtagttgg
    27241 taacatcaag aactacttta gcagtctccc cacgtttaac gctaaatttg aattttttac
    27301 catctagtaa ataggttgtt ttattagcat ctgttcccaa gtcggtttct aggaaataat
    27361 agtcacctaa ttccaaacca tctacagtta atttacctgt gctgtctgtt ttataagttc
    27421 caacaacctg atcagatcca tcatcttttc gcaattcaaa aacagttccg gcaagagcct
    27481 ttccatcttc gtctttttta tcaagctctg ctgagtttaa ttggaaatag tttttaggat
    27541 agacatgtac atcataatta aagtattgat catctgatcc gtttagttca ggtacgttaa
    27601 caatcatagg atacgcttct tcaactacac ctttgactgt tcctgtttca acaatcaaat
    27661 acaagttatc tgttaaatcg tctgtattta cttgtatagg tatgtctata ttcccttgtg
    27721 aatcagtctt cattctactt acttcactgt attttttgtt tagtaataat cgtgctttag
    27781 catcatcaat atgttgacct tttgtagtat cactatccgt ccatcccaag gatttagctt
    27841 catctttagt aagtttcaca taagagaact caacgccttc aagaggtttt acaccatttg
    27901 gtaaagctat ttctttccct ttgttatcaa tatctgagac agggttccca tccccattag
    27961 tatttgttac tttatggata tgaatcgttc cagttccttg ataattatag ttttgttgtg
    28021 catatgctac ttgcccccct agtccaaggc tagaagagat tgagcttgcg cccaatccca
    28081 ctcctagcaa agtaccaaat agcattaaat ttgaaaaaat tttttttgat ttcatacgtc
    28141 agccttcttt tttcatattt acttcttttc cattaacact tttttatttt gctttgtgct
    28201 gttctcgata atacagtagc accatactca atcctgcaat tagaaataca agaaggaagg
    28261 ctgatgtacc gattccgcct gttcttactg tttcaggtac aaggtgatta attactgtat
    28321 attcaaaggt ttgttcatcg tcagcaacgg tgatgccttc tttagaccat ttccctgtat
    28381 aaccattagg gttttgtgtt tcttcgattt tgtacgtttt gttggcaaca atattaaatg
    28441 ttgccaagcc attgctgtcc gttttttgta caacttttac acctttgtcg tctgtcaaag
    28501 tgaattctgc gtttgctaat gctttccctt gatcatccac tttatgaact ttgatcacac
    28561 cttttttcaa agtgttttta gcagtatatt caaagacttg tccatcatct ttgacggtaa
    28621 ttccttcttg tttgaatgcg cctgtgtaac ctgttggtac ttttgtttct tctaagttgt
    28681 aagttttgtt cgcaataatg ttgaacgtag ccagaccagt tgcatccgtc tttttaactt
    28741 cttttacacc tttttcatcc gttaaggtga attctgcatc tgctaaaggt tttccatctt
    28801 gatcgacttt atgaactttt actacacctt ttttcaaatt atttgtagct gtatattcaa
    28861 aagtttgccc atcgtcttta acggtgatac cctcttgctt aaatgcacct gtgtaaccta
    28921 ctggtactcc agtttcttct aggctatacg tcttgtttgc tacaatattg aagcttgcaa
    28981 gtccattttc atctgttttt tctacttgtg ttgcaccttt ttcatcggtt aaagtgaact
    29041 ctgcatttgc caaaggtttt ccatcttgat cgactttatg aacttttact aaaccttttt
    29101 tcaaagtgtt tgtagctgta tattcaaaag tttgtccatc atctttaacg gtaattcctt
    29161 cttgcttgaa tacgcctgta taaccagttg gtactccggt ctcttcaagg ctgtaggtct
    29221 tgtttgctac aatatcgaag cttgcaagtc cgttttcatc tgttttttct gcttgtgttt
    29281 gggatgaatc atccgtcaag gtaaattcag cattagctaa tggttttcca tcttggtcaa
    29341 cctttttaac ttttacttta ccttttttaa ttgtgttcgt agcagtgtaa ctaaattctt
    29401 gtccatcggt attaacagta ataccatcct ttttaaatga accttgataa ccttcaggaa
    29461 cttttgtttc ttctaagctg tatgttaagc cttgttgcaa atcaaacgta gctaacccat
    29521 catctccggt tgtcttaaca gatttagcat tgttattgtc agttagagta aactccgctc
    29581 cttttaaagc atttccatct tgatcaactt tcttaactgt tacctgacca acagctcttg
    29641 tccatttagc actcaaagat ttagagtcct ttgtttctga agtagtgatc ataactacgg
    29701 catcttgata cacacctcgt ccatcaaaag taaaaggatt ggttgtgcta acattagcag
    29761 tacctttaaa tgacaaagta ccacttggtg tagtcacagg tactttaata gtaaaacttg
    29821 aatttaattt cacagtgttg ttagtaattt ctttaccatc tgcatcataa atcttcatac
    29881 cagctggttt tgctgatgta aaagtgagct tcgcattacc agtcttattt gatgctgttt
    29941 tatatgtgaa agtgtgatat tttccttcgt cagctgtctt agaagctgtg atagaaaact
    30001 cagtgttggg cgtatctttt ccattcttag catagtcata aattttttca aatgcttttt
    30061 ttacacgagc tgtttctgcg gcggtatggt ttggatgtga ccatacaatc tgtgaaactt
    30121 taaagtttcc ggctaaaacc catgaaacta attgtgttgc ataccatgct tctgtatcat
    30181 ttcctgcacc cagttccttt gctgtttttg atggaaaacc acctttaaac atacgataga
    30241 actcatcact gccagctccc ttgtacttca agcttccggc tccacttcct gttggggatg
    30301 gcaattctga atccatacaa tatccaatat ttccatgaga atctttaaat tgacttgaaa
    30361 tcatccattc atttggatga ttcggcatat taaccctaat ataaccgtct gtttttacgc
    30421 cagtttctgt aaattttgca cttgtcggtt ttgtgacatc tgcttcagct cttattgctg
    30481 aaaaaggcat tataagcgta gataacacca ttactaaagt cgcaaaacaa taaattattt
    30541 ttgattttaa aattatcgtt ttttcatgct tcatctctcg ttactccttc tttataccag
    30601 ttaggcgaag aaaatgagcc acgatatgtc ggtgtggtaa tgaccttcgc gcccattcct
    30661 aagttaaaat caccacacct attaatcact tttttcgttg aaagcaagct gtttcaacgc
    30721 tctagtattc atagcatcac cccttttagt tctaccaacc atgagcaaca agttttctaa
    30781 gtagttaagc aaggacaaat aaagactata cttaactact tagtatataa acgatctctt
    30841 ttattaacgt accgaaaccg ctaattgaga tacgttatat gctgtttcaa atgatcctgc
    30901 attaatccaa cttgccccaa acacaggatc gacaacatgg taaaaaccgt ctttaaatcc
    30961 atcaacggct actacatgtc gattccaaat agcatcgccc caaaacattg atttatgttg
    31021 agcaggttca aaggcataag ttacccacgc tactacagga tttccattta caatctcatc
    31081 ttttaatcct tgtaccgatg aaccagaaat atctacaaca tttccgccat tagcaacacc
    31141 ccatgaaact acaggagccg gattcatacc ttgataagtt ccatccacat tgtgtctcca
    31201 ttcaccagaa aaaccattat ttggattatt atcaggagat agtggcattg atttaacaaa
    31261 agtcattaaa tcttgatttg ttgcaacacc tttataatgt aacgcttcta gcaaagatgt
    31321 tccttcgcaa agcattgtgt taccttgact aataaatgga acgcctagca caacattagt
    31381 tggttgttcg ggtttacttg gtgaagtaga actgtcagtt tcatcacttg aacctgtttg
    31441 tgattgatca ctagaagatg tactggttga ttcatttgca tcatttgatg ttgattgtga
    31501 gttatttgtt gatgttccag tatcagcttc cgtagtgcta ctttcacttg ttccctgtga
    31561 tgcttctttc tcacttgtag aattatttgt attagatgaa cttgctccat caacacttga
    31621 agcacttgtt tctgtcttat cagaagtgtt tgctgttggt tccgattctt ttgtagtctc
    31681 ggattttttg ttctcttgct ccgtattttc aacaggatca ggtttatctt tttgaacagt
    31741 ttgactaact acttcgccat ctgcttcctt aactgttaat gtatctttat tgatgttgaa
    31801 agttcgtcca atcgggatag tccgttgttc tccttttgcc aaatcaacat tgttcaattc
    31861 ggcaaactca gcaacagtag tcttgtaacc taagctttta agcttctcta cagttgacca
    31921 aatagtatct ccttttttga acgcataact tgattgtcct gttgtaattt ggatttgatc
    31981 cggtgtgttt gcagtccatt catcagcatg tgaggttact ggtactgata accccgcaag
    32041 tacagctgat gcaaataaaa ccatagcaag cgaatttttc ttcatattca ttccttcttt
    32101 ttgtttttat tttctgtttg tgatcgcaat gcttctattc cttcttccgt tataatatag
    32161 gttttgtcac ccactttctt gaacgaacct ttaggcaaac gatttggata atagttgatt
    32221 atgtttgtaa cataatttct acctttaccc aaaattagac ttgcttccct tgcggtatac
    32281 aagttgtatt tgtctaggtt caatttcttt ttatttgcga tttagaccta cccctttcgc
    32341 aaaatttatt ttataataat agtaccattg cactatcata attgcaataa ttttttgggg
    32401 tttatcttca tttaatagcc taaaaaggct tattagataa ggaatatcta actaatcaaa
    32461 acaaaaaagg aagttaagaa gaaatgaaca aaaaaaggaa attatcagta ttaattacac
    32521 ttgtttctat gttggctatt cttagtggga tcttgtcgat taaaattttc aatcaaataa
    32581 atacaagcgc aaataccaaa aacgaaatag tcatagaaaa tacatctaat tctaaagaaa
    32641 ttagtactac taaaaacaaa gacaaatctg atgaaaccac tactaaaaat tatctaataa
    32701 agactgttta ttttgatgaa cggaaaacag caattctcaa agatgaggaa ggatcagaat
    32761 taaagctacc tataacggta tccgataagt tcaatggaac gttctcctta caaaccgctg
    32821 aaggatcacc tgataatctc aaagttgggc aaacaatcat tcttggtaaa gacgaagaac
    32881 tgtattcatt tgtaaaataa aaaaatggag caaaggctag tcaagccctt gctccatttt
    32941 ccattcttca agaggtgtag ctatttcaac accaaagttt aaatactgtg gatgctgtag
    33001 atacatgata ttcattctat tttcgataaa ctcttttaac gttgagtaaa tatatttttc
    33061 taatgctata tctgcccaac tatcataatc aagtctattg gtgatattga cgtttatttc
    33121 aatttgagaa acatcaatat tttgaacatc aggaaactta ttcagtctta gctcttctct
    33181 aaccgcttct actagcggtt catattgtcc gtcttgttcg ctaattgttg aaactatttc
    33241 atctgaattc caacgatctt ccaatacctt tgctacattt tctttaggcg gttgtctttc
    33301 tataaattct tcaggtgttt catcaaactc tactctaaag ttcaatttat tttgattgtt
    33361 agcgtacata taatcatctc tgtttgattc aaaaaagtta cgaattcctg tatctgattt
    33421 atcacttaac tccttatttg ctaagttagc ccaaaaatcc caagttcctc tattttcagt
    33481 atacaaagta aaattaatgt ttatataatt tgcagtttca ggataccctt gctctctaag
    33541 agcatcttta acaatcaccg ctaagtcagt ttcaaaattc tccataaaaa attcactaaa
    33601 ttcttttgga tcccacgttt cttctaataa cttttctaat ttacgatctg ccattattat
    33661 tctcccccac tttactttct tatatctaaa actgcttaaa attaagagct ataacttatc
    33721 atagaaggtt ttttatgatt tatttaagtc tacaattcta tattctaatt atcatctaga
    33781 ccattttctt atgtcaaata aaccatatat attgtcttta atagttttat ttgacttttt
    33841 aagaaagtat ttacttttga ctttttttac gttttacaac atatacgtat tgtaatgttc
    33901 agctcaattt attataatca aaatgaattg attaataaaa attctgaggt tcgggttttt
    33961 tatcaattct tttctgtttg tgtttgtgat gaaacaatcc tttttcggta ggttttgtta
    34021 tcatcgtcag cacaaagagc taacgttggt accgttagct ctttttttat tttactctgc
    34081 tcttacatag ctatctaaat cctccaagta aaacaattga ttttctccta tgcttgttac
    34141 tccacgccaa attgttgttg ttgctgttgt tggagtattt cttgtctgtg taatcaattc
    34201 aaccatttta cctttttgat ttgttgtata gtaatcagca gtatagctag atattggact
    34261 attaccagta cctaccattg tgccagagat atcaacagga ctagacttta ctatctgagg
    34321 aaattctttt tgtatttcct tcatattctt cgtatatttt tcccatgaat tccagttgta
    34381 aaataaatta aagaatttcc tactttgttc cttgatttca gcatttccca attctttgta
    34441 ttgggcttct tgcatagctt tcactttctg gttattcttg actacttcga tttgtttact
    34501 tttaaggtct tccaattctt tgacattatt atcataagca tttttctttt gaaatagtac
    34561 cacacaacaa aggatcaaaa ttatggaaag tattccgatc gcctttttag cagtagtaat
    34621 ttcatgattt tgcataaaaa gcacccccag tcactttctt atcattattt agtgtgatgg
    34681 ttaatgtggt atcatacact ttttttccgt tgtattgaac ttcgaaatca gtcaatgctt
    34741 catacaaccc actactagat gtcttaattc caaagtttgt tagcttaaaa gtaaaaggtt
    34801 tatagtattc tattttttct tgctcgctac tctcgttagt aactggtggc ttacctgtca
    34861 caataaataa ctttcgcaaa aattcttcac ctgactgatt cgcaaatttg cttcggtctg
    34921 atcctttaaa catctcttga atatcatttt ctaaatctct ttgcaatcca tccacttcat
    34981 ttggtttaac ctctttaaag aaggtcaatt cattttcaat ttgcacactg tgcttttcag
    35041 cttctatgtc ttgattctgc ttattgaaat atagaattcc aacaagcatt gctaatagca
    35101 taccagaaag caaagaaatg ataattgcat ctttcgtttt actattcatc tctgtcgccc
    35161 tccattcaaa tttttatttt tcaatttcaa atttagcttt gcctacaccg ctaaagaaat
    35221 gcacattatc tgacccatcc acaccatcaa tcaaagttac tgaaacacct cttgttggtg
    35281 cttgggtagg tggaacattg aaattagcgc acaaaaatga tccatcgtca aacaccgcta
    35341 taactacacc tgtatgtcct gctgatccaa cagcccataa ccatccatct aaagcactaa
    35401 acgcataacc tacaattggt ttgttcgtca cctttgctcc tgccgatttg tagctttccc
    35461 aaacgtaacc gccatttccc aaagttggtt gatttccatc atataattga ctcatataag
    35521 cccatgttaa ttcagtacat tgtccttgta attcaccttg aaaaggtgat gttgaccagt
    35581 ctggttcttt aaattttggt agcgtgattg ctttcccata tttttttaat gcttcttcat
    35641 aagtcatatt tgctattact tgaccttttt taccgttaat ttttccttct ttcccaattg
    35701 atccagaacc agaattagca tcatccgtag ttgcacaggc atcttctact gttcctgatg
    35761 ctgaacttga tgaattattc gatccatctc cggtacttga acctccggat attttattga
    35821 tcttactttc atcttttgca ccgtccatac ccttgctttt catgtacttc cagatttctt
    35881 ttgcattttc accacgtaca gctattacag ctgattcgga atcagctgaa atttcccagt
    35941 ttttatgaaa agttactgca ttttctacta catcatcaga agcatttaaa gcataatctt
    36001 ttaatattcc tacgaatccc ccatcacctt tgaacataaa gtccaattgg gcttctgtag
    36061 tccaccaaga accgccatta tttttcttag accaatcaac taatgctgta tgtcgcccgg
    36121 ctgtccattg accatagcca atacccatta gaccatcagt aaattgtttg atgaagtcaa
    36181 cttctctctg cccatcgccg taataccctt ctactgttcg aggatcaata cctgattctt
    36241 gtttccagtt acctaaaata cctgcaatac tagcacctga taagccatat tctccattca
    36301 tgtatttgta aatatcttca atatttttgg ctacatcacc tgttacagcg ccacttccag
    36361 tatcgtttcc atcgctatct gttcctgacc cactaccttc agtagtacag ttaaattgtg
    36421 catcataagt agctatccca tcagcatgta acacttgcgg atttattgtg attacactca
    36481 ttgcaatccc tgtaaactgc accattaacg taagcaacgt ggcaatcttt ctcttagatt
    36541 ttttcaataa ctacgccccc aaaaagtgta cttttttatg ttattatgat aatataataa
    36601 cataaattaa tagaaaggaa aagctaataa atgactgata aaacaagtga tttacctaaa
    36661 ctagatccaa aaatatctca agttaagagc aaaaaagatg gaaaaactaa aaatgctgta
    36721 aaggaaacat cctcgttact acttgatatt ccaatgagtt cttttgatga aatacttgat
    36781 gccaatacgg atgaaacttc tgttaaaatt gcatctcacg ttggaatgaa cagtcaaaat
    36841 gattattttt acaacttgtt aaacagccta agaaagttgg aagataaaaa aaatcaggta
    36901 atcgcttcaa aagctcctga cctcacaacc gatttggatg ctgaaattat ccaaatcaaa
    36961 caagatgagc gatcagttaa atcgctactg aaaacctact ttgatggttt tgataaagaa
    37021 ctagctcctt atagccaagc aatcctaact aaaccaatac aagctccaca tatttcaatt
    37081 tccctacaaa aaaagagtga tgactaaaca gtcattactc ttttttagat ttctggtaag
    37141 cgctgatcgc aaaaaccatc aacaatattg actcaaaaaa tatgttcatt cttttctctt
    37201 ttttccatta tagatttccc tctattccgt taaattgaaa gtcacaccga ttactacaaa
    37261 gattaacatg accacattca aaaacattaa aagatatgct gataatctca tcattttttt
    37321 agttcactta ctggtaatct aaacctttga gtgattgcac tggaaatacc atattcacca
    37381 attacaagca aaaaatacgc aataatttca gttccaccta gatagatcag taaaataagt
    37441 aagtcactta acattgttag tttcctccca gtatatgatt ttcatttttg ggaagtcttt
    37501 tataatccaa ttcctcaaaa aaaggaacca tagtgtcact aatataggtt ttagtttcag
    37561 gtactgccaa aattccccaa tcaagaattt cttcccatga tacatcatca aattctaaaa
    37621 tattatgatc aactgaccta ttaccaagtt catatccacc aaaaattacc atattatcaa
    37681 tcatgtatag aatttcatcc ggcgaaccat gagttaatgt ttcgtcctct tcaagcagtt
    37741 cgtttaatct ttgtttgtat tcttctcttt tcatactttt atcccttctt actcattttt
    37801 ttgaatttta acatttttaa tccaagtctg cttggtatct ctgtagtagt catgcgcttt
    37861 tattgtcgcc ttaacttttc ttatttctcc aacatcccat tcatcataat aaagaaattt
    37921 atttgtcttc caaatcaatg aattatggtt ttgatcttca aataaatgca ggtaaacatt
    37981 gccccattgt gtatctatgg tgacggtatt ggtaagtttc aaatccaatt ccaatcgttg
    38041 agcgattgta ccaataaact caccctcttc tctacgcttc gcacttaaaa tctcctgttg
    38101 tcttttgctg tattcttcta gcttcaactc aataattcct tcatcattga ttacagaaaa
    38161 ttcttctaca aaaacttttt gagttttgta ttgttcttgt ttttccttaa aaaaaccaac
    38221 ctaataaagg atcataagta gctccttgat gatgcagttc atctttgatc gtatagctat
    38281 ttttctctat aaccacgtaa atataaccct tttcaaagcc ataacgatca aaatttttct
    38341 gagattcttt ctctcttgcg atccttattt ttcgatcagc tcttttttta tttaaaacat
    38401 ttagcttagt tctatattct ggcgtaaagg atttttcagt tacaacaaga aatccatcac
    38461 catcgcactt aaaacatgtg ccataggtaa ttttccccat agacataaac ttgtactgac
    38521 ctgatccgct acaacgatag caagggattt ttttcttcat gattatagtt ccatttttat
    38581 ccggttgcga tttgctgatg atttcttctt gactgaacat cctccatact cctttctgag
    38641 cgaggataaa accttgctca tcttcaggtt gtttaaaaac taggatcgta gtatttttct
    38701 ctatcaccta tataaaaatg aatatcttct ttaagcactt tccatccatt ttttgtctta
    38761 actaactcca atatttgacc ttcaggattt ctgctatatt cccactcttg atctggaaaa
    38821 gtagctttat tgattgctcc atccgcttga accaaaattc tgtttttgct ttttacttca
    38881 attactgtat aaggatttct gtcggtatat agaagctcgg ttgctcccat tccaactaca
    38941 atctccttgt ttgttgtact gccttccatc attctatttt gtaagctacc ttctaatttc
    39001 atttaagatc accctttctc aacttatcta caaattcacc atacataccg atacacattt
    39061 tgtaaataaa ttaattttat aaatagataa aaaacgatgc ctatttagca tcgtttcatt
    39121 tgttaatgtt taattataaa atcggacttt aggagaatga tctggaaaaa tagactctgt
    39181 ttctatttct tggatgagag aaactctctc ttccatcaat tgtttatact ttgatgattc
    39241 tataatttca cttctagatt tatttaaaca cctcatagat tcataataac taaacattct
    39301 atttctattt ctattttcat ttaacagttc caatctttcc tgatttcttt tcttaatacc
    39361 ttctatttct ttcaaataat ttatcccctt ccataccaat tatgatgcca cattggatga
    39421 aataatcaca tactattttt gaccaaataa ctggtcaatt tagaattatt tacaccaaat
    39481 atccttcaga ttttttacta cttcataata ttcttgtgag tcttgaacct cttttaccgc
    39541 tacaaccgct gtcaagtctt taggatgaaa tctaagtgcc gatggtttta tcatactcca
    39601 tcgagtattt tctaatcttc ccttagtttc aagtaaagct acttgaatga tattgtctct
    39661 tttattgtag taaaaagtaa acttttctgg gaacttaatc attgagttta actcttcaaa
    39721 ccattctttt gatccaaatt caacttctac cattttccta tctcctttaa tttcattttt
    39781 cgtctattgt taattaattg cattgcgttc ctttatacta gttagataat cgtttatttc
    39841 agctatattt ttgtctgcat accattttgg ataaagcatg taagcatgac tatctttaca
    39901 tgctattaat ttttatctcc attttgatat ttctgctttt ttctactcac caatcaaccg
    39961 tagatcctca acattctcac cttcaagcga atatgagtat acttcgccgt aaatattttc
    40021 tataactacg acttcttctt caaagtctat ggactgcaca tcaaaccatt ctcctccgcc
    40081 aaattcgtct atgattaata cctgcaatat tttatttact gccatttcaa tttcccctta
    40141 taccattcta tgtcttccaa actatttttc ttctttccat accaaggttt cacgtatctc
    40201 aaaattttct ttaaataagt gttccggtac ttttataatt aactcttttc ctacttttat
    40261 cagatagtct ttttcaccta atgagatttg accagtcggt aactgtatcg aaactctttt
    40321 agttttatta aattttttag ttttcaatac agtatacggt ctacagaatt tctctaagcg
    40381 ttcaaatctt ttactatcgt attggaaata agctttagga acagataaag tacaaaaact
    40441 atcggtaaca gcacttatgt cttttttaga aattatccca actttagaac ttctaaggaa
    40501 agttgctaat ttcttatatc caccatgact tgtttcaata tagatgctat tgtattggct
    40561 gtcgtctatc tcatttattg tttcaacctt tttcctgaca ggtgtttcaa cgagttctcg
    40621 aacctcttct tcactgtacg ctaatacaat taactcttcc ccatttacca tctttagaac
    40681 atacgcttgc ttcttttttc ttggcttatt ttgagattca acataaccag acctggaatc
    40741 tttatacttc caagcatgta cttttttctc taatttctgt atctcttttt tcagctgtag
    40801 atattcagac attttttcat tgtaacgttc gcaatataaa caatattcat cttttccaaa
    40861 atcatgatca agtccttctt caatatgttt attcaacaaa ctagaaaaat attgattata
    40921 ttgctttttt cgcaatttaa gctctcgtaa atctttagac atttcatttt tcctttccaa
    40981 ttattagtgc cgtttcacaa tttgtaattg actaaacaca ataattaatt tgtaagctaa
    41041 caccaattac aactgttagt aattgggtta gtctctatca gattatctgg tgaatttttt
    41101 tattaaacaa atttcatagc tttcaaaaat cgctttgctt gaaaaggtgt tattctacaa
    41161 accttattcg ccaactttca atctgataaa gcaatttgcc aatgaacaat gaacttctta
    41221 ggaatcgttc tattattaaa caattagtga attgctcaaa gttctaatca ctccctctct
    41281 tttattatac acactaatgt gtataatatc aataattttc ttttcttttt taatagaata
    41341 tgtaatctat caaatattga tacaacattt gtaacgatgc tgttcgtgat caaattgtta
    41401 gcaatactca tttcaacaag gaaatatcgt tcttagaaaa attctgcact tcttaattat
    41461 gaagcagtca tattttatta ttattaaaat tgtttagcta tttaattgac tagtggctaa
    41521 aattatagag accaagtatt tacttggtct cacattctct tatatcattg gttaattaac
    41581 taccttcctt tcaaaatatc aagaattttt tttgcttttt ctttgtcttt tgtatagaac
    41641 aaactatgaa tgtttccatc aattccttca aagtgaacag cagattcttc attttttcga
    41701 aaccattctg tcgatgagct tagcacatca tctgtagcaa ctctcctata attcgtgtcc
    41761 ataattccat cttttacttt tgtaaggtat atatccccac taacttctgc gactactaat
    41821 tgtaaatttt cgtatgccat attttcttac ttctttcttt tttagttgct taattctact
    41881 agataacatt gattattcag agacttcaag taacacatct gaccatgttt caagttagtt
    41941 atctctgatt ttagtgatga aatatagttc acctaaaaaa caatgctccg aaaaaagcta
    42001 attcgaactc tatatttttt tgcagtgtca tcaatttttg tcaattggac tattaccttc
    42061 tttttcttcc aaatagtttt gtgcaagttt ccttactttt ttcatcatca aattcgagag
    42121 tttttgatta tctttttcgc tgtaaaataa tctactcgtt aaacttttag gataatcttt
    42181 gatagaatat gcatcccaaa cgattctttg aacatactca cgatctatat tttctctgac
    42241 ttccccagtc caaaaatgtt gaccataatg gcatctttct attacattgt tagcagaaac
    42301 ttcaaaataa gattgttccc tttgtattat catgccaacc atcatagcgc aggaaataac
    42361 tattaataac aagcctgttt ttaatttatt attccacatg caaattccac atccttctct
    42421 ggataaaaat ctccttgaca cttttaaatt acttttttaa atataaccac caaaacaaga
    42481 tcatgctgat aaatccaagt gtatacaaga taataaattc tttgttaatt atccaaaaca
    42541 ctctgatttc ttttagcttt tcacgcatta gaaaacacct tgttatatct tttttgtatt
    42601 tctttcaatt gttttttaaa actcataaac aactgttcat gatctggtct aagtgttaaa
    42661 atatttaaat tatttatctt tgttaattga gtaataaaat ctattttaga ttttctttca
    42721 atatcaaatc caacaataat ctttcctgtt ggatcaacac atttcacttg aatagctttg
    42781 tcattttgtg atatctctaa tattgatgat ttttcttctg cttcttccaa aagatgttcc
    42841 aacacttcgt taggtgttcc atgatagtaa taaatcatgg tcttttcgaa aatttttatc
    42901 ggatcaacac catattcttt tccgattcga taaaaaaatt tcattggaat ttcttccgga
    42961 tttctgttcc tgcttgacca attagagatg gtttgataat ttgcaaaatg gattcgttcg
    43021 gctaacctct gaactgtcgt ctctaaatct ttttctatta attgttttac agcaccaatg
    43081 ttttgtgaca tttactttct atcccttcaa attcgttgca ttatcaattc taagatttca
    43141 tgttttaacg catgtctgtc tacattttgc ttttttaact ctgcactata atatcttcga
    43201 atccctttat ccaagcatat aattccttga tctaatagtt catattccgc tttttcaagt
    43261 cttctctttg cctgatcaac aagcaaaatg atattgtgat gtgtctgatt aaactgctga
    43321 tcatattcct ctaaagtttt agcatctaag ttagcaatat gatcctctgc taatttttgt
    43381 gttaaaaata attcgacttt ctcagatttt aacatctgta ttttttcata ctcttgtttt
    43441 gcttggatat agtcatcttt caagctagac atgtaactca tctccttacc tgtataaact
    43501 gatacggact gtccaaaatt gaatagattg gtgatgactt caagaagact tcaccatttt
    43561 cacatgaaaa taaaccaatt aattcttcaa tttcatttac gatcagatat tcattagtta
    43621 aaattttccc attttctttt tctagttcga caatcgcttg attccatgta ttttgcacta
    43681 actcttgaat caatttcaat ctatccattt ttttcatctc atacagggca atgtcagctt
    43741 gtttaatgcc ttttaatttg cgaaaggcat atttatattg tgtgtagttt aaaaagcctg
    43801 tgtagttata catgggcttt cctgatgcct tgtattgaat taagcttggg tatatttcag
    43861 atttccactt tttctctgaa aattcgtcaa taattcttaa ctgatcaggt aaatcaacat
    43921 cgtagtattt ttcaattatt agttcccgat gaagtagatg tttctccttt tctttttgga
    43981 ttacaaaaga agaattttca ctcagtctac ttatttcttc ccttgttaac ccaagtctta
    44041 ttttttctac ttcagtttta agttcacgaa tactttttgc ctttaaccac ttcccatttg
    44101 caccgttatt gtaaccggta agtaactgta ttttataaat tccttcacca tgaagcacct
    44161 tatatatacc aacctgattc ttttgcggaa tatgagcttc atttgcactg atttctttca
    44221 ttacgcattc tcctttataa tctagttaat tagcttgtgt gtttcgagta gttgagtaag
    44281 aacgtttgtt tcagaaatca tttatttgtt atcacttaat tttaaaaaaa tagggcttac
    44341 gccctaaaga actatttaac tttggattga tcgtccataa caggttctga ctgttgatat
    44401 aatttatatg ctcccaaatc catataatat ttagttgtac tttcaatcat ttcatcagtt
    44461 gtatttgttg tcattcccat tacatcccag tatagtgtgc cattaacttt ctttggtgtt
    44521 actactgcaa ctgttggcgt atgatattga ttgttctcat cagccagacc aggaatgttt
    44581 tgtatccact caggtacacc atcagtaata tctacagcaa ctgcgtgata ctcgtaagct
    44641 ggtttattta tggtaatatc atgcattaat gttggagcat aactaaatcc ttcttttatt
    44701 tcttgcatga tttttgattc tgctttttga caatctggac aatcaggttt gtaaagaacg
    44761 atagtcagat ttttattttg caatggatca ttcccataat cttcatcatt ttttactgta
    44821 ttaataaagt cggtataaac ttcttccggc ttttgacctt tattaatgtc ttcgtatttt
    44881 tgatactttt cagcattatt ttcagtcatt gaagaaattg ttgttgatgc cgatgtagag
    44941 ctgtttttag taaccaccgt actactagac tttgtgtctt tgttattatc agcactatta
    45001 gttgtaccac atgctgtaag agttattaat gctgctgttg ataacaatac aatcttccat
    45061 ttagttgatt tcattttatt cacctatcct tttttcgttt gtttaacact aaaagaataa
    45121 cccgtactat ccaataaaac aacagcatca atatggaaac ccaaaccatt tgcacaatcc
    45181 aagcataaag actaaataat aggtattgtg gatttttttg aatgattgta tttgagatgc
    45241 catttcctac agtaaaatac attgttacga aaataacacc ttcaattatc aagtgatttg
    45301 ttggttgatt aataattttt ttcatatttt tactcctaaa ctaaatttat tcttcctgct
    45361 aaatattgaa aattatatgg gttctgcaca tattccctaa gttccttaat acttgtatca
    45421 atatcaccaa aggctgtttt aatgtttgct gacccatctt tatatacaat catttcccct
    45481 tcacccatac agaaagaaat ttcagtatta gaaatgattg ttttttcagc acgaccattg
    45541 ataatttctt gaccatcacg ccatataaaa tacataagat aaaatacctc ataagcgttg
    45601 tatccagtat ccgttgttat tgataccaaa acatcagcat cttgtattcc tgataaagct
    45661 aatccaattt catggatcac atcaacttca ttttcttccc aatctccact ttcgaagacg
    45721 gtataggttt gattgttagc aaatggtttt ctctctatat cgaacattcc tttccaatca
    45781 agaatcatgt acccgattaa attgtgaaag aaaattaaat aaaataaata caataagatg
    45841 ctaactacaa aaatatcagc cattattgac ataggattat caggatataa ccagatgtaa
    45901 aacgattgtt tcaaatccat ttttccatca tctcgctcca tatatttatg ctatcatgat
    45961 aacataataa cataaatttg tctcgctttt taatattttt ttatggtaaa cttgtcatac
    46021 aaacaaaaaa aggagattta tatgtttata ttagatgatg aaataaaaaa agttaaaaat
    46081 cctagaatta ttccattatt agaagaagtt cgaagtagtt tttatcaggg aaattatcgt
    46141 tctgcaatag ctgtcaatta cagtgcgctt gttttagatt taattgataa attatctgat
    46201 ttagcaaata tttacgagga tattacttct aagaaaattt tagaagatat agctagatta
    46261 agaaaaagtg atccaaattc tccaaaatgg gaaatggaac tactagaaaa agcttatagt
    46321 tcaactgagc taattgatag ctatgaatat gaggatttaa aatatattaa ggatcaaaga
    46381 aattattcag ctcatcctgt agtcacttac aacggtaata cttgggaatt aagagatata
    46441 acaaaagaaa cttgtcagga tgttatacgg aagtcctatg aaattatatt cctaaaaaat
    46501 ccaatattag gaaaaaaagt aacgatcgaa attatacaat atgcaagtcg catacatgct
    46561 atgtctgtta cccctgaaga atttcactct gcactaaaaa atagctatct aaataaacta
    46621 agcgaaaaag caaaagaaaa cctatgtaag acagccttta agtttgtttt tcaattacct
    46681 tatggaaatg aggaagcaga tagagatagg gaaagtactt tcagactact taatgcccta
    46741 atttttgata ataaagaatt ctatttaaaa attctaaaag aagaaatttc aaagtatagg
    46801 ttcacagctg aatcaagaga ggaaattaat aaatcacttg gtgaaaatca acagatcaca
    46861 tatacaaaag gggtcttttt acttactttt atagctaccc atccagaatt acaacatgat
    46921 ttttcagacg agacaattca aaatttacaa atatcaataa atgattttaa accgaaacaa
    46981 caagtactaa cagaaataga atattactac cgtttacgtt cacttgcagt tctaggaagc
    47041 aacaatgaag aattacaaaa tcacttaaat agtctacttg atcctatact tcagcaaaaa
    47101 atgagagttt cttcgctacc atcttcaaca ttagaaacaa tatataatca atatttatat
    47161 tttggtgaaa aagagatgtt catagatttt atgatcgaac atcttaccga tgctagttca
    47221 ttcaggttag cagataagga tatggaagta ttaccatgga tttacaataa attaaatcag
    47281 gagcaattta acaaattact atacaaccta aatggaaata atcaatttta caaaaacagt
    47341 aattttccgc cttttatttc taatctgtta gacttttata atcagaaatt ccaagtgaat
    47401 ttacgtaaac attatagtgg gcttctatat ccaaatgcag tgactatgga tcagggtttt
    47461 caattaaagg ataaagaatt aaaaaacctg tatgaattag ctgaatcgaa aagtgatatg
    47521 tatctagaca taatagataa tattattaat aagtctgaag actatttaag aaaagaaatc
    47581 aattccgaga aagaattgtt aaattatgtt cacaaaataa aataatttct tataacacaa
    47641 gatgcacttt tatagcgtat atttactttc aaattaaact aattagttat attctcacaa
    47701 tagcatttaa taataaatat actgtcgctt gatttatata tttccctctt ctttctttag
    47761 aatctctctt tattttgata taataaaata gaaatcgcac caaacaatga aatggcggaa
    47821 aacatttcat tgaaaaaagg attgaacata atgttcaatc cttttttata gttataatca
    47881 attaacattc ggtgtgtgaa attgaatctt cgatgctttc acatggaaaa ttattgattc
    47941 cttcttcact catttcccat gtagtataga aaggagttaa ctgtcctcca tactcctgaa
    48001 taataaaata ttttccatca ttgatatggt tacaacagta cactccaata gttggatact
    48061 cgactactaa aaattggtct ggatcaaact gttctttttt caacatttct cgtatttgaa
    48121 acaaatgatc cttctctaag caatcaggta attcctttat tctttgcgtt actaatgacc
    48181 cctctatcgc tttccattca tttaaatatt gtttcataac aattcattcc ttttatgtta
    48241 actatttttc ctaggaaatt gttttaaaac gccactctcc cagaaaacgt aagatttggt
    48301 tagctagaat ataccttcca gctaaccaca attttccatt actatattgt aagcttgctt
    48361 tcacttcata aaaattgctg atatactctt ttcaaatcga actcttccta tctgagaagg
    48421 gtatttcttg cgctagcccc tcataagttc catcttcatt ttcccaaatt tctacctgaa
    48481 atactgaatt attggctaat ttttgttgca agagataata gggttcttct tcatactttt
    48541 gatcgacttc tttaagacat tcaatagcat caatatcaat attttgaaag cccaattttt
    48601 ctagataaca atcgtaccaa gtaccccatg tattatccag aaatgagaca aatgcatgat
    48661 ccatatcatt tgctataaaa gattcatctt cccacctgaa gaggtaatcc cactcaccgt
    48721 ttgcaatttt agttctgatt tcattttctt tttttaactc aaaaggatta caccaacaat
    48781 gctcatgtcg catccaaaca aaaaattgtc cattctcatc tattttaaat tggctgagat
    48841 cacctttcaa tctaaataca cggttctttt ctggtaactt attcatgctt caaccacctc
    48901 atactgttct ctcatacctt tgacttcatt aaaaaaaact ctcgcttttt cttcatcagt
    48961 aaagttttta tacaactgat tacaataatg aggtttttga agtttctgaa tacctatact
    49021 aacttcaaat ccttcttcat gttgaattag gattacttct gtgtctctac ctagaaatat
    49081 tcgatctaaa atttttatgt ttggtttcat tatttaacct ccgctataat tatttcactc
    49141 agtaaggttt cgctttatga aatttctata gttctctttt cacaaaacgt accatgttct
    49201 ctaagactgt ttttagtctc aaatatacat gttttttctt atcctttcct tatacactca
    49261 aaatcaaaga taaaacttaa agaataaact ctccaataac aaaattatta gtcagatcca
    49321 ctggtctcct gatctgaaat ttattacttc ttcctgttgt caaatattgt ctcaaatcaa
    49381 cagaaagttt ttgatcatct tctccttttc tagcaggagc aacttttttt tcatacttca
    49441 ttaactctcc actggtcatc tttccaatga tagtgtaatt aaaatcactt tcaaaacttt
    49501 ggtaaatttt tccatctgta ttgaatatat cagctgtttc aatatcttct ttgtattcat
    49561 tgtcttctcc tgaaccgatt gtatcaaata gaaaggcaag acgaattcca tcttcttcaa
    49621 gcattgttaa tcttttttta attagcccaa cggtttcaat agataactta tcgaagccgt
    49681 gaatcataac aatatcatct ttttttgctt gtgatgtgat gaaatcaaag gtatttaaaa
    49741 attgagcttc cataacattg atttctgctc ttagatcacc taagttatag taaaactgta
    49801 atttgtcact tttaccgatt tcttttagtg tcgttctttg attaaacatc ctagagaagc
    49861 tgtctaatgc tgttctcaat gtgtctttta atacaataat acgatcgact tctttttcag
    49921 taccagattt tttggctttt gtttcagcat tagtcaaata gctaataaaa tccccaagaa
    49981 ctggcattgt atcactgctg ttcaccaaaa gttttgaacg ttcttcataa ttaatatccc
    50041 aaagtttctg acttatataa aagttaccta attctttttt caataaaact ctagttgcag
    50101 aatctaatgt tctgcctgct aacaaataaa aaatctgagt tagtttatcg agattccgtc
    50161 tatataaatc accaatgtcc ttttgattac caaaaagctc caaagagtta agaccttctt
    50221 cagccaaatt aaccttttct attacactgt ttaacgaagg tttggcaaca aatgttctac
    50281 cagttggatc accaaagtaa tcaaatttat ttaagacaat atgaaaagtt cgtttatgat
    50341 ttgccattgc attattagca atcaattgcc cccaaagtga tgatgctgag attttttcat
    50401 catagccttt aattgtagcg tttttgtgtg atgccactaa aactttactt gtaaaagaat
    50461 catccagtgc cataaaagct acaccacttg cgtagctagt agacatagtg ccgatagccc
    50521 atccattcgc atcatctaaa cctttacgaa ttgcatgatc atttcctgca aaatccgaac
    50581 tcatagacgt atactcatag acattcccga ttggttcact taaaagtcta tcaaacagat
    50641 cacgttgatc tcctccatat gacataagct ttaatccata cattttttca tcatacaacc
    50701 gttggagttt aattagctgt tcgctgattt tattaggaga atcggatact aataggattt
    50761 ctagcttcca atcaataatt ttatttgcca tgctgtctag taaagatgct gtattcaaat
    50821 ccataatccg cataacagtt ttgctaattt cagcatctga attttttcct tctaaattat
    50881 tttcataact attcgttgat cccccaacat tctcgtgcat tatttcttgt tgatcttttt
    50941 gtgacactgg cttatctgct tcaattagat aggcttttac cccttctagt ctcacttcag
    51001 gaatagcttt aaccatccaa ccaaatccat aatttctatt cattccataa gtattcacta
    51061 atttcacaat tgttccccac cgcttacctg tcctaatgta agatggtgta aattgataac
    51121 caactaaaga tgttaaatca ctaacaaagg tattattcac tacctttgaa tttctagttt
    51181 tttgttcttt tttagttttt ctcttttttt tgaaatttaa tagttgtgat ctacccataa
    51241 tttccattta atctctcacc tctatttcag tgcataaata tcagataata tttcttttgt
    51301 ttgtttagct gacaactgag tgacactata ataaagtcct ctactcgtat agttttctaa
    51361 acgttccaca ccttcatcca ataccatctt agattgagat ttcaagataa gatattggat
    51421 tactgttgtt ttcacaccat caatatattc tgatacaaat cgttcttgct gatctattat
    51481 gtcaaagact gctttgttac tccttgcatt tcgtttttgt attttcatat ttcgagtttg
    51541 tcgttccgta ttctgaggtt gagatgaagt gattttgatt tcagctaagg aacgatctct
    51601 ggtattttga tattgtattg atttcgattc ctgagccata acttcttctg gaaaagcggt
    51661 tagtgatgta gccccatcta gattgaacat taatgctaca ctcccatctc gatatacgat
    51721 tgttccatct tgtttcactt ttaaaatctc tgtgcctcga aatggtttct ttttacccat
    51781 agatactctt gctgaattaa ctactgattt gaacagtcta atagaaccag aaatcaatga
    51841 accactagat ggatcaataa acatatactg gctaattaga ataagaaatc caattatcat
    51901 taataacgtt ggaacaaaaa taaatttcag tttaatcgta tcagaaacta agtaaacaat
    51961 tgatccaaga acaacgactg caacaactat agctgataca attaatcttt ttgctgtaac
    52021 agaataggtg ctattgacat gcttttcaag ccattcaact ctgacaattg tttcagaagg
    52081 tttttgatgt aagttatgtt taaatccata gtttgagtta tctgccatca gaaatacttc
    52141 ctttcacgtt caacttacgg taaacaccta cagttatcac tattgcaaca ataagcaagc
    52201 caattaaaga tattgacttg ctcattgtct cgttcgtttg tggtagttca tttacctgat
    52261 acacgagctg tgcttcctgt aagtgctgag aagaggtcat gttttcttga ttgttttgaa
    52321 ttactggata aactattttt tcttctcgca taatagccct ccttagttat tatcagataa
    52381 gtgagcttga attttgtact gatttccgtc tggagttttc acaactgctt tttctccatc
    52441 aatagaaatc acttcagcct tagttgtttt cccatcaata ctgatgacaa catctttccc
    52501 aactttgaac tctgtagttg gtgttgcttc atagctacta tttgttgaag cagagttcgt
    52561 atttgtccca atcgaatttt ccactctcga aatagatgtt tgagtttgag cctgtgcttg
    52621 ttgattatta tgactaataa agtaggacga tcccaagatc gttccgcaag ccattgcagc
    52681 gacagcaatg atagtagcca tctttttact tgtaaaatca cgttttgaac ttgattgatt
    52741 ttctgtagca actgctaatg atttcgtttt ttctgccatt tgagctagat cacttcggga
    52801 acgagataag tcttctctta aagcttcact ttgttctgcc aacttttcgc tatttaattt
    52861 ataaccctct gctaaatctt tgacctcttt cattcgatct ttatattctt cgatagtttt
    52921 ttgttgctct acaagttttg tttctaattc tttcgtgttg ttagctacct gcttttcaac
    52981 ttcttctttg ttcgcaaaat atttgacaac cacatctgtt gttttctctt ttgtatcatg
    53041 gaactcagct tcattttctg actttgtttt ttcgacaatc tctaaagttt tttcttgcac
    53101 gtctgttaat gatttttcaa tttctggttt caatgaattg ataattcgtt ccaccacaac
    53161 ttcttttgct tcttggcttt gtttcaatgc atcgttttta atagattcaa ttgcttcttg
    53221 gtactgttga tcaagacttt cttcagttgc ttttctatat tcatcttctt gttgtttaac
    53281 ttctggtaaa tgttcagcat cataccgttg ctctagtttc tttagttcag catccaaata
    53341 agcctgtttc gccatatcat aatctttacg aatgatattg gatttttcca tcactaaatc
    53401 atcaatttta gatgcttctg cttcgtagct ctctttagca accgatagtg tatttgtaaa
    53461 ctgattttga ccatttaatg aggttgcatc ctctatccgc aaaagctcat cggctaacat
    53521 ttgctttcct ttgaggatca attgctcctt cgtcttattt aattcactgt tgattttatt
    53581 attagcaata gagatacgag cgttcatact ctctttagct aaaaacagca atggtttcaa
    53641 ctcaggtatg gatagcatct cctcgtttaa atcgacaggg tcattaatct catctaaaac
    53701 tacaggttga aatttgtcag caatgatctg ttcaaaattt ttttctaaaa aatcagcatc
    53761 taagtttttc ggtacatcac tttcagatgt gattttttca gcttcataaa cataattttc
    53821 tttgtcactt tgggattcgg gtttttcatc aatttttggt tgttggtagg tttctgtttg
    53881 tatttgtgtt tcttctatgc ttacttcttg gttttctgct tcggttggtt tgttatcatc
    53941 cacaaagaga gcttcttttt catctggtac atcttctgct acggcttcaa attcaacttc
    54001 ttcagaagag ttgaaggtct cagcaattcg ttctaatcca ttagtagcta cttctaattg
    54061 ctctactgcg ctatagaagt ctgtagcttc ttcaacttct gattcaaaca cattaggatc
    54121 atccggaata actaataatt gaccactact atccataaaa cggacacctg ctaatagctt
    54181 ctctttaaat ttttctggta gatcagctac ataattcaaa cttgacttca atagaacgac
    54241 atacttttca gaatcgagaa tagcatcatc ctcatctata actccctcac tgttgagctt
    54301 ttgtacatct tcaattacca tataattttt ctttggtaaa tctttggtca gcaacgcatc
    54361 gaattctttc catacctttg ctttagcttt ttttgccttc gcttcttttt cttctttttt
    54421 gtacacttcg agtaattgtg gcttcaaaga gagtgcaact acaggaactg tttctgccac
    54481 ataattaata gattcggtaa tatctttgaa ttttgccatt attgtaaaac tccttttttc
    54541 agtcaatagt tcttttctgt ttgtctagca tatatacata tgctttttcg aatttaataa
    54601 gcgtttcaag tctggctttt tcaatctttt tactcccatt tcgataatca tagaactggt
    54661 taacattcat attcattatc tttgccagtt cggtaccttt cagactgctc attaatactt
    54721 cgttggcgtg agctaacatt ttttctctat caaaaaccat cttacattta ctttcttccc
    54781 tcacttctaa actacgcttg gagcgtagtt ttttggtaaa aagtaaattg aacaaatcgg
    54841 catattatcc aaatttgttc aattcacttt taacctaact gttcattttt tggaaatttt
    54901 gaaactagat agataccaac aactggaata agtgctgata tgatattagc tttttggttc
    54961 aaatcattac ctgttttagt acccattgtg taaagcataa cggttgtaat tacaggaata
    55021 ataaagattg caataatcgc cccaacatat tttaatccat ctccccattt tgcatcacca
    55081 aaaaatttca ttgcagtgaa tcctggtttc aacgaaaaaa cgaatgccaa gaaatacaaa
    55141 acaacttgta ctaaagtttt catatcaggc gtcattccgc taaccaaatt tttaatagaa
    55201 tcgaacatat aaaaacctcc tttttaatat ttttaataag ttaattatat catcgacaaa
    55261 tcttatttgt tcacttgtca atgatctaac taagaaaaag acgtctcgat atttacatct
    55321 ttatgttatc ataataacat aaattttcga tgattaaaag agattttcca ttaaattatt
    55381 tcactttttt tattggctaa aaatgctggt ttatcaagtt tttgtttaga taataattct
    55441 gttctttacc agcgagagt
//
LOCUS       DACECY010000013        54954 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.013, whole genome shotgun sequence.
ACCESSION   DACECY010000013 DACECY010000000
VERSION     DACECY010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.013"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            493..1200
                     /locus_tag="IWO38_000807"
     CDS             493..1200
                     /locus_tag="IWO38_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0312914.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1259..1903)
                     /locus_tag="IWO38_000808"
     CDS             complement(1259..1903)
                     /locus_tag="IWO38_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ0312915.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            2030..2317
                     /locus_tag="IWO38_000809"
     CDS             2030..2317
                     /locus_tag="IWO38_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312916.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2425..2745
                     /locus_tag="IWO38_000810"
     CDS             2425..2745
                     /locus_tag="IWO38_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0312917.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2826..4139
                     /locus_tag="IWO38_000811"
     CDS             2826..4139
                     /locus_tag="IWO38_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0312918.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            4136..4855
                     /locus_tag="IWO38_000812"
     CDS             4136..4855
                     /locus_tag="IWO38_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ0312919.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            4983..5534
                     /gene="raiA"
                     /locus_tag="IWO38_000813"
     CDS             4983..5534
                     /gene="raiA"
                     /locus_tag="IWO38_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ0312920.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5756..8290
                     /gene="secA"
                     /locus_tag="IWO38_000814"
     CDS             5756..8290
                     /gene="secA"
                     /locus_tag="IWO38_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ0312921.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8416..9517
                     /gene="prfB"
                     /locus_tag="IWO38_000815"
     CDS             join(8416..8478,8480..9517)
                     /gene="prfB"
                     /locus_tag="IWO38_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ0312922.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9622..10308
                     /gene="ftsE"
                     /locus_tag="IWO38_000816"
     CDS             9622..10308
                     /gene="ftsE"
                     /locus_tag="IWO38_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ0312923.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10292..11185
                     /locus_tag="IWO38_000817"
     CDS             10292..11185
                     /locus_tag="IWO38_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0312924.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11337..12785
                     /gene="cls"
                     /locus_tag="IWO38_000818"
     CDS             11337..12785
                     /gene="cls"
                     /locus_tag="IWO38_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ0312925.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13122..13979
                     /locus_tag="IWO38_000819"
     CDS             13122..13979
                     /locus_tag="IWO38_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ0312926.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14094..15014
                     /gene="pstC"
                     /locus_tag="IWO38_000820"
     CDS             14094..15014
                     /gene="pstC"
                     /locus_tag="IWO38_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ0312927.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            15014..15898
                     /gene="pstA"
                     /locus_tag="IWO38_000821"
     CDS             15014..15898
                     /gene="pstA"
                     /locus_tag="IWO38_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ0312928.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15913..16719
                     /gene="pstB"
                     /locus_tag="IWO38_000822"
     CDS             15913..16719
                     /gene="pstB"
                     /locus_tag="IWO38_000822"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ0312929.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16735..17493
                     /locus_tag="IWO38_000823"
     CDS             16735..17493
                     /locus_tag="IWO38_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ0312930.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17506..18183
                     /gene="phoU"
                     /locus_tag="IWO38_000824"
     CDS             17506..18183
                     /gene="phoU"
                     /locus_tag="IWO38_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ0312931.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18421..19452
                     /locus_tag="IWO38_000825"
     CDS             18421..19452
                     /locus_tag="IWO38_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ0312932.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            19449..20216
                     /locus_tag="IWO38_000826"
     CDS             19449..20216
                     /locus_tag="IWO38_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ0312933.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            20316..21884
                     /gene="liaX"
                     /locus_tag="IWO38_000827"
     CDS             20316..21884
                     /gene="liaX"
                     /locus_tag="IWO38_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ0312934.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            22184..22501
                     /locus_tag="IWO38_000828"
     CDS             22184..22501
                     /locus_tag="IWO38_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ0312935.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            22501..22851
                     /locus_tag="IWO38_000829"
     CDS             22501..22851
                     /locus_tag="IWO38_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ0312936.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            23018..23956
                     /locus_tag="IWO38_000830"
     CDS             23018..23956
                     /locus_tag="IWO38_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ0312937.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            23971..24801
                     /locus_tag="IWO38_000831"
     CDS             23971..24801
                     /locus_tag="IWO38_000831"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ0312938.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            24845..25870
                     /locus_tag="IWO38_000832"
     CDS             24845..25870
                     /locus_tag="IWO38_000832"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ0312939.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            25885..26823
                     /gene="galU"
                     /locus_tag="IWO38_000833"
     CDS             25885..26823
                     /gene="galU"
                     /locus_tag="IWO38_000833"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ0312940.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            26948..27388
                     /locus_tag="IWO38_000834"
     CDS             26948..27388
                     /locus_tag="IWO38_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ0312941.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            27391..27993
                     /locus_tag="IWO38_000835"
     CDS             27391..27993
                     /locus_tag="IWO38_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0312942.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(28052..29158)
                     /locus_tag="IWO38_000836"
     CDS             complement(28052..29158)
                     /locus_tag="IWO38_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ0312943.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            29368..30369
                     /gene="ccpA"
                     /locus_tag="IWO38_000837"
     CDS             29368..30369
                     /gene="ccpA"
                     /locus_tag="IWO38_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ0312944.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(30706..33051)
                     /locus_tag="IWO38_000838"
     CDS             complement(30706..33051)
                     /locus_tag="IWO38_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ0312945.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            33330..34232
                     /locus_tag="IWO38_000839"
     CDS             33330..34232
                     /locus_tag="IWO38_000839"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ0312946.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            34445..36220
                     /locus_tag="IWO38_000840"
     CDS             34445..36220
                     /locus_tag="IWO38_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ0312947.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(36286..37872)
                     /locus_tag="IWO38_000841"
     CDS             complement(36286..37872)
                     /locus_tag="IWO38_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ0312948.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            38122..38346
                     /locus_tag="IWO38_000842"
     CDS             38122..38346
                     /locus_tag="IWO38_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ0312949.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            38488..40239
                     /locus_tag="IWO38_000843"
     CDS             38488..40239
                     /locus_tag="IWO38_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0312950.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            40239..42023
                     /locus_tag="IWO38_000844"
     CDS             40239..42023
                     /locus_tag="IWO38_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0312951.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            42349..43200
                     /locus_tag="IWO38_000845"
     CDS             42349..43200
                     /locus_tag="IWO38_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0312952.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            43254..45005
                     /locus_tag="IWO38_000846"
     CDS             43254..45005
                     /locus_tag="IWO38_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0312953.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            45009..45179
                     /locus_tag="IWO38_000847"
     CDS             45009..45179
                     /locus_tag="IWO38_000847"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312954.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(45219..46121)
                     /locus_tag="IWO38_000848"
     CDS             complement(45219..46121)
                     /locus_tag="IWO38_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0312955.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            46352..47755
                     /locus_tag="IWO38_000849"
     CDS             46352..47755
                     /locus_tag="IWO38_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0312956.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            47755..49188
                     /gene="ascB"
                     /locus_tag="IWO38_000850"
     CDS             47755..49188
                     /gene="ascB"
                     /locus_tag="IWO38_000850"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0312957.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            49333..50259
                     /gene="trxB"
                     /locus_tag="IWO38_000851"
     CDS             49333..50259
                     /gene="trxB"
                     /locus_tag="IWO38_000851"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ0312958.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            50473..52200
                     /locus_tag="IWO38_000852"
     CDS             50473..52200
                     /locus_tag="IWO38_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ0312959.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            52272..52565
                     /locus_tag="IWO38_000853"
     CDS             52272..52565
                     /locus_tag="IWO38_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ0312960.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(52657..53517)
                     /locus_tag="IWO38_000854"
     CDS             complement(52657..53517)
                     /locus_tag="IWO38_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ0312961.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            53609..54037
                     /locus_tag="IWO38_000855"
     CDS             53609..54037
                     /locus_tag="IWO38_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0312962.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            54199..54375
                     /locus_tag="IWO38_000856"
     CDS             54199..54375
                     /locus_tag="IWO38_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ0312963.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            54402..54848
                     /locus_tag="IWO38_000857"
     CDS             54402..54848
                     /locus_tag="IWO38_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ0312964.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT        17840 a         9616 c        11763 g        15735 t
ORIGIN      
        1 gttagttcga gagctctttt ttttgctttg ctaccttgtt ccttgaagat tctgctgaat
       61 taagatagag ttaagttaac tacaagaatt ggagggatcc acatgaaatg gaaagaaaaa
      121 gtagacgaag cagctgaaaa gttatatgat ttggtcaaat cagaaaaata caatattgaa
      181 gtgaatattc cgaaaaaagg cggaaaagcg gtccaggtaa aatcaaaacg cccaacgaat
      241 catacaaaaa aatggatggc taaaaaccgc taataataac aaaagatcca taagtcggca
      301 ttttcatcga ttttagggtc ttttgttatt ttctaggttg aacatatcta aaaaattgtt
      361 tctgacaata ggagtaggga gaacgatgtg tgtctaaatc ctctgttgat ggatagaaac
      421 ctttatcttt ttcagttgtg aatcaggtat ataccagtta taatagaaga aacgaaagga
      481 cgggtgtacg ttatgtcgaa tcaattacct gtatatatac agattcatga ccaattaaaa
      541 aatgaaatcg aacagggtat atggaaagtc ggagaccgtc ttccttcaga aagagaatta
      601 gcagtgaaat tcaatgtcag ccgtatgacg ttgagacaag caattcaaaa cttggcagat
      661 gaaggaatct tagaaagaaa gatcggttcg ggtacgtatg ttgcgcgaga aaaagtacaa
      721 gaaaaaatgt ctggtgcaac aagttttaca gaaattatgg aatcacaaaa tcgagtaccg
      781 tctagtcgga caatctctta tttcttgact tcgccaagct caagtgaaat ggaaaaacta
      841 aacttgagta aagatgacac gatcttgcga atggaacgta ttcgtttcgc agatgatgtc
      901 cctatttgtt ttgaagttgc cagtgttcca caaaagctga tcgatggcta cagcaaagca
      961 gagattactg aatcgttgta tcgcacgcta gaagaaaaag gcgggaaaaa gatcggtcca
     1021 gcgaatcaaa cgattactgc tgtgttggct tctgagaaaa ttgctgacta tttagaggta
     1081 aaacgaggag atgcagttct tcgtttgcgc cagatttctt attttgaaga cggtacgccg
     1141 tttgagtatg tgcgtacgca atacgtagga aatcgttttg agttttattt ggaaaaataa
     1201 aaaacaggtg atccctgatt ccttattaga aataaaagag aatcagaatc acctgttttt
     1261 atatatgatc agttatcagt tgctcgtgat aagaaacaga accctcttca aattggactt
     1321 gtccgttcag cagctcaatg atggattctt taaagtggtc agcacttttt tcatctacag
     1381 cacagatgac gctaacttga tcagtatatt ggatttcttt tataaaaaaa tgttggagtt
     1441 caagataatt ctgtaatttt cctagttgcg gatacgtgat catcacagag atctcctgtt
     1501 gcaaggtacc ttctacgatt ccaatgtcag acaatgcttg agagaccgca tgagtgtatg
     1561 ctcgtattaa tccgcctgtg ccgagtttcg ttcctccaaa atatcgagtg actactgcag
     1621 ctacattgat cagttgattt tttttgagga cttccaacat cggtacgccc gccgttccac
     1681 ttggttctcc atcatcgctg cttcgctgga tttcattttt ttctccaatg atgaaggcgc
     1741 tgcagttatg gttcgctttc caatgttctt tttttatttc ttgaatgaaa ctttttgctt
     1801 ggtcctctga ttctattcgt ctaagagaac agataaagcg tgattttttg atctcaatct
     1861 cactttgtcc attttctttt attgtacgat agcgctcgat catcttgtca cctctttttt
     1921 tcattttctt tattcagtat aaataaagtt ctaggttgtt tcaacactgt aacggttgcg
     1981 caattgctgt agtatgcgat aataaagaca aaggcaggtg gatctgggga tgcaggaacg
     2041 aatgaaaaag tacgatgcaa ttacgcatta tttgaagaac aatggcggct ctcaagtaac
     2101 gttgaccttt acccaatttg acgaactgct gtttcccagt aacggacttc ccaaaacagc
     2161 aagggaaagt acagattggt gggcaaatga ttataaacac cctgaaaaag gtgcctatgg
     2221 ctggataaat gcaggctatg aagtagttgt catcaatttg gacaaagaat atgtcgtgtt
     2281 caataaatta gtgaaatcta gttggctatt tgattaaata aaccgcgttt agaaaacgtt
     2341 gacaaatttc tttcttttta tataatagag taacagacgc gaagaaaacg tttgcatagt
     2401 ttgttttaaa aaggagagaa aagtatggct aaaaaaacaa tcatgcttgt atgttcggct
     2461 ggtatgagca caagtttact agtaacaaaa atgcaaaaag ctgctgaagc aaaaggaatc
     2521 gactcggata ttttcgctgt gtccgcttct gacgcagata acaaccttgc aaataaagac
     2581 gtggatgtgt tgttattagg cccacaagtt cgctttatga aagctgattt tgagaaacgt
     2641 ctggaaccaa aaggcattcc tttagatgtc atcaatatgg ctgattatgg tatgatgaat
     2701 ggcgaaaaag tgttagatca agcaatcacg ctcataggtg aataaataga acagtataac
     2761 tatcgaacaa gcgatccaaa aaatagatcg cttgtttttt cgtatatttt tttagggcga
     2821 agaatatgaa tcatttttgg ggtagaaggc ttctgacgag agatattgaa gcaatagact
     2881 gtgaggaggt aaatttacca aattatgaga agatcgcagc agtggaacgt ttgggcaact
     2941 ggattctttg tcatcgctgt ggaatgagga cgtccgtcat tgatggacag cttgcagatt
     3001 acggttattt ttgtacacat tgtctatatt tagggcgatg cgacactcaa caagatttat
     3061 atttgtttga tcagcctgaa gcaaaacctc gagaagtcgt ctttttttgg acaggacagc
     3121 ttactgaaaa acaaacggaa atctcaaaga gaatactttg ccattcagaa aaaagacatc
     3181 atcttatttg ggctgtaaca ggtgcaggga aaacagagat gctttatcct attttagtgg
     3241 aaactttaaa agctggagga agagtagcga tttgtacacc gcgagtcgat gtttgtaatg
     3301 aattgttttt acgctatcgt tatgtttttc cagaagaaaa aatcacgttg ctccatggga
     3361 atacagcaac ggcttatacc tttacttctt ttgttatctg tacgattcat cagctatacc
     3421 gtttttatcg gtcctttgat ttaatagtga tcgatgagat cgatgcgttt ccttataacg
     3481 gggatgcagg attagctcat gctgtgcaaa cagcgattaa accagacgga cgattcatct
     3541 acttaagtgc cacacctgat aaaaagttac taaaggaaat caagcagacg tttgacatcc
     3601 acaaacttgc tttacgcttt catcggcgtt tgcttcctga acccgtcttg gttttcatga
     3661 ataattggcg acaaaaatgc aagaacaaga agaagataaa atctttggtg cggatcattc
     3721 atcaattaat taaagaaaac catatccttt tattttgtcc aagtatcacg atgatgcatc
     3781 gtttgaaagc cacacttcaa gaggctcttc cgagatatga gatcacagat gtttcttcaa
     3841 gagacaatca gcgggcggaa aaagtccaga aaatgcgaga agcaaaatat gatatactgt
     3901 taaccacgat gatccttgaa cgtggagtca catttgaaag aatatccgtt atcgttttag
     3961 gagcggatca tccggttttt actaaatctt ctttggtaca aatcgcagga agagccgatc
     4021 gtaaagggcc ttatacgaac agtcaagtat actttttcta tgaagaaaaa actgcagcta
     4081 ttcgaaaagc ttgtcaggaa atcaaagaaa tgaatgaaga agggaagaag atgctgtgag
     4141 gtgcagttgc tgcagtaaag atatcttcca aaatttaaca ttcagcgaga ttattggtgt
     4201 ttcgaaatac agattacctc agacttgttc agtttgtcgc gcacaatttc atttattgga
     4261 aacaagaggc tgttctggct gtcaaaaacc aatccatttc aatctagaga gtacgaaaaa
     4321 gaaagtaaag tctattcccg tttactgcag tgactgtcaa gaatggaaga agaaatatcc
     4381 gacctatgat tttcagcacc gtgccttttt tgtgtacgat gaaaatatga aggcctggct
     4441 caatcaatac aagtttatgg gagatatccg actagcaggc acctttgcat ataaatggac
     4501 gcagataaaa aaagaatacc cgacatacat ctattgtcca atacctttat ctgaaaaaag
     4561 gcgaacagaa agaggattca atcaagtaag cgagatgttg aaagcagcaa gaattccttt
     4621 tcagatgcta ctcaaaagag agaagcatct gctggctcaa gcaaaaaaca cgcgaaagga
     4681 aagattggag atgcctcaac cttttgaact tgcagtcacc ttttctaaga tacaaaataa
     4741 aaaaatattg attattgatg atgtctatac aacaggacag actcttttcc acgcagcaga
     4801 ttgtttgcag gtcgcacagc cgaaaatcat ccgaaccatt tctttggcga gatgaattag
     4861 atgtggaaat ttgatagcga tttaatttgc aaataaccta gagttcgata taatagaatt
     4921 aagaagaggg aaagagtggt ccttctcctt cttgattgtg gtagacgaaa ggggtaattt
     4981 ttatgtttag atataatgta cgcggcgaaa atatcgaagt aactgaagcg attcgcgact
     5041 acgttgagaa aaaagtaggc aaactagaac gctatttcag tgacgcaccc gatgcaacgg
     5101 cgcatgtgaa cttaaaagtt tatacagaaa aaacagcaaa agtggaagta acgatcccat
     5161 taccttatct tgtattacgt gccgaagaaa cttctccaga tttatacgca agtatcgatt
     5221 tggttgttga taaattggaa cgccaaatcc gcaaattcaa aacaaaaatt aatcgtaaat
     5281 cccgtgaaac gggcatgaat actgcagatg ctgctgcttt attcaatgaa gaggacgaaa
     5341 acgaaaatga ttctgaatta gatatcgtac gtacgaaacg cctttcattg aaaccaatgg
     5401 atagtgaaga agcagtctta caaatgaaca tgctgggaca taacttcttt atttttgaag
     5461 atgcagaaac aaatggtaca agtatcgttt accgccgtaa agatggaaaa tacggactga
     5521 ttgaaacaga ctgatcagcc ttacttaaat aacaacgaaa acttgcagca gctactgaca
     5581 gctgccgcaa gtttttttga gtttcctcct cttttagatg aaatatgaat aaattatgaa
     5641 gttcttactt taatacgctt aagatatttc aattatctat aactaatgat aaaatgaaaa
     5701 gtaaagtcta catagtctgg ataatacaca gaaggaaaag gagattacac aacgcatggc
     5761 caatttgatt cgttcactta tcgaaaacga taagaaagaa ctaaaacgtt tagatagcat
     5821 tgcaaaaaaa gttgaagcat acgctgacca aatggcagca ttaactgatg aacaattaca
     5881 ggcaaaaact gctgaattca agggcagata ccaaaaagga gaaacattag atcaattact
     5941 tccggaagct tttgcagtcg tccgagaagc agcaaaacgt gtattggggt tatatccaca
     6001 ccacgtacaa ttgatgggtg gaatcgtgct tcacgatgga aatatccctg aaatgcgtac
     6061 tggtgaaggt aaaaccttga ccgcaacgat gccagtatat ctaaatgctc tgactggcga
     6121 aggggtacac gtagttaccg tcaatgaata tttagcaacc cgtgactcga cggaaatggg
     6181 cgaactttat aatttcttag gattaacagt ggggttgaat atcaactcaa aatcttcgga
     6241 tgaaaaacgc gaggcttata attgtgatat cacttatagt acgaataatg aattaggatt
     6301 tgactacttg agagacaata tggtcgttta ccgccatcaa atggtacaac gtccgctgaa
     6361 ttatgccatt gtcgatgaag ttgactcaat cttgattgat gaagcgagaa cgccgctgat
     6421 catttcaggg caagcagaaa aatccacagc tttatataca agagcagaca acttcgtgaa
     6481 acgtttgaaa gaagaagaag attataaaat cgatgtacaa tcaaaaacaa tcagtttaac
     6541 cgaagcaggg atcgaaaaag cagaagaaac attcggtttg gataacttat acgacattga
     6601 aaatactgca ttgacccatc acttggatca agcattgaga gccaactata tcatgctttt
     6661 agatatcgat tatgttgtcc aagacggaaa agtactgatt gttgaccagt tcactggtcg
     6721 gatcatggat ggacgccgtt attcagatgg acttcaccaa gcaatcgaag ctaaagaagg
     6781 cgtagagatc gaagacgaaa cgaaaaccat ggcgacgatc actttccaga actatttccg
     6841 tatgtacaag aaacttgcgg gaatgactgg tacagctaaa acagaagaag aagaattcag
     6901 agaaatttat aacatccaag tataccagat tccgacaaat cgtccggtta tccgtgatga
     6961 tcgtccagat ttgctttacc caacattaca aagcaagttc cacgctgtcg tgcaagatat
     7021 caaagatcgc taccgcaaag gtcaacctgt gttagtcgga actgttgcgg ttgaaacatc
     7081 tgagttatta tcagatatgc tgaatcaaga acgtattcct catgaagtgc tgaatgcgaa
     7141 aaatcacttt aaagaagcag agatcatcat gaatgctggt caaaaaggtg ctgtgacgat
     7201 tgctaccaac atggctggtc ggggaacaga tatcaagtta ggacttgggg taagagaact
     7261 tggtggtcta gcagttatcg ggacagaacg acatgaatct cgtcgtatcg ataaccagct
     7321 tcgaggacgt gcaggacgtc aaggagaccc tggtatgtca cagttctact tgtcattgga
     7381 agatgatttg atgaaacgat tcggttcaga acgaatcaaa tctttcttag atcggatgaa
     7441 gatcggtgag gaagatgctg ttatccaaag caagatgctg acgaaacaag tagaatctgc
     7501 acaaaaacgt gtagaaggaa ataactatga cacacgtaaa aacgtcttac aatacgatga
     7561 tgtgatgcgt gaacaacgtg aagtaatcta taaacagcga caagaagtaa tcatggaaga
     7621 aaaaagctta tccaagccac taatgaacat ggtcaaacgg acaatcagtc gtgtagtaga
     7681 tgcgcataca caattagaga aagaaaattg gaatttggaa agtatcatcg attttgccgg
     7741 cagtactctt gtccatgaag atagtattac tcttgcagat atcgaaggaa aaacagctca
     7801 ggagatcaaa gagtatctaa acgaacgagc acaagaagta tttaatacca aagcggctca
     7861 actaaatggt ccagagcaac tgttggaatt tgaaaaagtc gtgatcttgc gtgttgtgga
     7921 ctctaaatgg acagaccata ttgatgcaat ggatcaatta cgtcagtcaa tcggcttacg
     7981 tgcttatgga cagaacaatc cattagtcga atatcaaaca gaaggctata agatgtttga
     8041 agatatggta ggcgccatcg agtatgaagt cactcgaatc tttatgaaag ctgagatccg
     8101 tcaaaacgta cagcgtgaac aggtttctca aggaacagct tctcactcag aagacggaga
     8161 tgccaaagaa aatagtaata ctagcgcgaa aaaacagcca atccatgtga acaaggtcgg
     8221 aagaaatgat ccttgtcctt gtggaagcgg gaaaaaatat aagaactgtc atggaaaaaa
     8281 tgcaaactaa tttctgacag caggattggt gagacaaatt ctttttttaa gaattcgtct
     8341 caccttcttt ctgttatttg ctgattctgt tagactagat ataatgaaaa aagtatagtg
     8401 aaagaaggaa aaaacatgga aatatccgaa attcgaaatc tcctgacaga aatgcaggaa
     8461 aaggtcacaa gcttcaggag gtctctttga cttagaccag ctggaagaag acattgcgga
     8521 agcagaagca aaaatggctg aacctggttt ttgggatgat agcgaagctg ctcaaaaagt
     8581 gatcagtgaa aacaatgcaa ataaagaaac ttatgatcaa ttccattctc ttgctgaaga
     8641 actggaagaa atcgaattga tggtggagat gttgcaggaa gaacctgatg cagccatgca
     8701 agaagaagca gaaacacgta tcaatgcgct agatgaaaaa atgaagacat atgaattatc
     8761 tatgttgtta gatggtcctt atgatcgaaa caacgccatc gttgaattgc atccaggggc
     8821 tggcggaacg gagtcccaag attggggcag tatgctttta cgtatgtata cacgctgggc
     8881 agagcagcat gggtatcaag tagagacact agattatcaa gcaggtgatg aagcaggaat
     8941 caagagtgtg accctactga tcaaaggaca caacgcattt ggttatctga aatcagaaaa
     9001 aggtgttcat cgccttgtac gtatctcgcc ttttgactca gcgaaacgac gtcatacttc
     9061 tttttgttcg gtagatgtga tgccagagct tgatgaaacg atcgatatag aaatcaatcc
     9121 agatgatttg aaaatcgata cgtaccgagc aagtggtgca ggtggtcagc acatcaataa
     9181 aacagaatct gctgtacgga tcactcactt gcctacagga acagtagtag ctagtcaggc
     9241 acaacgttca caattgaaaa accgtgagca agcgatgggg atgttaaaag ctaagcttta
     9301 tcaattagaa gtggaaaaga aagaacaaga agcagctgca cttcgaggag aacaaaaaga
     9361 aatcggctgg ggttcacaga tccgttctta tgttttccat ccatattcta tggtaaaaga
     9421 ccatcggaca aacgtagaga caggaaatgt ccaagcagtt atggacggtg accttgattt
     9481 atttattgac ggctatttaa aaatgcatct acaataagaa cccattgtta aattgaaata
     9541 aaattgtaag attttgcaag catgttgaaa cattgccatg ctataatagc taaagactga
     9601 aaaagatatg gagagaacgc catgattgaa atgaaggatg taatgaagaa gtactcaaat
     9661 ggtacgaccg ctatccgcaa tctctcaatt gtcatcgaac aaggagagtt tgtctatgtg
     9721 gttggtccgt ctggagccgg taaatctaca ttcatcaaat tgatgtatcg agaagaaaaa
     9781 gcttcaaaag gaactttgaa cgtcgcaggt catgatctaa tagcaatcaa aaaccgcgaa
     9841 gttccttatc tacgcagaga aatcggcgtg gtgttccaag actataaatt attaccaaga
     9901 aaaacagtat atgaaaatgt tgcgtacgct atgcaagtta tcggcagaaa acctcgggat
     9961 atcaaaaaac gagtaatgga agtgctggac cttgttggtt tgaaacacaa agttcgagtc
    10021 tttcctagcg aattatccgg tggggaacag caacgtgtct ctattgctcg ggcaatagtg
    10081 aatacgccta aagtacttat cgcggatgaa ccgacaggaa atctggatcc agaaaactca
    10141 tgggaaatca tgaaattatt agatcggatc aatgcccaag gaactactgt cgtgatggct
    10201 acacataaca gtacgatcgt aaatacgatt cggcaccgag taattgctat cgaaaacggc
    10261 cgcattattc gagatcaagc ggaaggggaa tatggatacg atgattagaa cctttttccg
    10321 ccatttatta gaaagtatca agagtttgaa acgtaacggc tggatgacag ttgcttcagt
    10381 cagtgcggta acgatcacat tgactttagt cggtgtattc atggcagtta tcatgaacgc
    10441 aactaaatta gctcaagata tagaaggaaa cgttgatgtc tctgtattcg tagatatcgg
    10501 tacaaaagac gaagagatga aaaagctaga aaaagaattg aatgacttag atcatgttaa
    10561 aaaagttgaa ttttcaagca aagaacagga attgaaaaaa atccaaaacg aaatgggtga
    10621 ttcctggaaa ttgtttgaag gtgacaataa cccgttatat gatgtgtatg tagtaagtgc
    10681 tactgatcct tcttatacaa aacaagtagc agaagaagca gcagatttga agaatgtttc
    10741 tcgttctgat tacggtggag cttcctcaga tcggattttc caaatcgctg gtgcagttcg
    10801 gacatgggga ttagttgcag cggcattgct gctgttcgta gcgatgtttc tcatttcgaa
    10861 cacgattcgg atcacgattt tgtctagaca gcgtgaaatt caaatcatgc gtttagtcgg
    10921 tgcaaaaaat ggatatattc gctggccgtt tttccttgaa ggtggctgga tcggtctgat
    10981 cggtgcgatt cttccgattt tgatcatcac atttggttat gcatggtttt tccaattgat
    11041 caatccatca ttattacgtt cccattattc attgattcat ccacaaaata tcgtttggaa
    11101 aatcaatctt ttaatggtcg gcattggtgt gatcattggt tcacttggct cgatcatttc
    11161 tatgagacgt ttcttgaaat tctaatacca caatataaat aaaatgagaa agttggacag
    11221 tttcgttcca gctttctttt tttattggct aggagaatag aaggaagcgg cttttttccc
    11281 tttactttaa aaaacaactt gggtattctt aagttggtag aagaagaggt gaagccgtga
    11341 aaattatcgt tgacaatttt tttacgatcc ttttgattat gaatattttg ctttccttta
    11401 tcatcgtttt tcgagaaaga aaggaaactg ctcaaacatg ggcatgggtg ctcgttttga
    11461 tgttcattcc agttgtagga tttattttat atattttttt aggaagaggg atttccaaag
    11521 agaaaatctt tgatttgaag atccaaggga aaatcggtaa gaacctggaa attgaagaag
    11581 acaaacaggc attgatgcga gggctttatc ctcatccgcc tactggacaa gtagatgtga
    11641 aacaattgat ctatatgctc acggtatttg aaagtacgct ctatacgact aaaaatgaaa
    11701 tcactttatt tacagatggt cgtgaaaaat tcgatgcgct gattgaagat atcaagcagg
    11761 cagaagacca tatccacttc cagtattata tttatcgcag tgatgcatta ggtgaagaag
    11821 ttcgagatgc attgacagag gcggcaagac gaggggtaaa agtcagagtc cttcttgatg
    11881 catggggttc cacacaagtt tcttccagct tttttcagaa tttgaaaaaa gcaggcggag
    11941 aagtcgcatt tttctttcct ctatttgttc cgtatattaa tcctcggatc aattatcgca
    12001 atcatcgtaa aatcgtagtt attgatggga aaatcggata tacaggtggg ttcaatgttg
    12061 gaaatgaata tctaggattg gtaaagaaat ttggttattg gcgagacaat catttgcgta
    12121 tttacggaga agcggtgtat accttgcaaa accgtttttt gatggactgg aattctcagc
    12181 atacaaaaga agtaggctat tctcccaaat tttttccgtc cattcactct actggtgata
    12241 tagctgttca aatcgtgaca agcggaccag ataccgaaca tgaacaaatc aaaatgactt
    12301 atttgaaaat gatttcgtta gccaaacggg agatattgat ccaaacgcct tattacatac
    12361 cggatggatc gattcatgaa gcactgaagt tggctcttct ttcaggcgta aaagttcata
    12421 tccaaatacc taacaaacca gatcatcttc tcgtttactg ggcaacgtat tctttcgctg
    12481 cagagctaat cgagtatgga gcaaggatcg aaacatatga gaatggattt attcatgcaa
    12541 agacgatgat tatcgatggt ggcattgttt cagttggctc agcaaatatc gatgttcgtt
    12601 catttcgctt ggactttgag gtcaatacac tggtttatga tgaacgaatg gcttcacgtg
    12661 taagaaaagc tttttttgaa gactcgaaaa tttctacgca tctcacaaaa gaaatgtatg
    12721 aaaaccgagg aataatcatc aagatgaaag aaggattagc aagattgatt tcgccgctcc
    12781 tttaaaacat aaaaaggaca tgagaaccat caacagatgg agatctcgtg tccttttcta
    12841 acggggccat ttctgagagt acgtcagaaa tggagaaagc gtctcgcatg agcgaaatta
    12901 actttggggt atctcttgtt ttttggggaa agcaagaaat aagctaatat atgctattaa
    12961 aagagacaca tttcaaagcc agtatagcat agaaactttt aatgagatag ttttttttac
    13021 aattatgaga atttattttg ttatttaatg attttgattc gttttcatca agtaatgaaa
    13081 atgataaaat aaacatactt taggattaaa ggagatttct aatgaaaaaa cttaaattat
    13141 tcttaccaat aataggatta gctattcttt tatcaggctg ttcaaagtgg attgatcgag
    13201 gagaatccat tacggctgtt ggctcttctg ctttacagcc tttagtagaa acagttgctg
    13261 aaacttacca gacagaaaat cctggaaaat tcatcaatgt acaagggggc ggaagcggaa
    13321 ctggtttgtc tcaagtacag tcaggagcag tcgacatcgg aaactctgat ttgtttgcgg
    13381 aagaaaaatc ggggatcgat gcctcagcat tagtcgatca tcgagtagca gttgttggga
    13441 tcactcctat tgtgaacaag gatattggcg taaaagacat cagtatggaa aacctgaaaa
    13501 aactatttac aggcgagatc aaaaactgga gtgaagtcgg tggaaaagat cagccagtag
    13561 ttatattgaa ccgcgcttca ggaagcggaa cccgtagcac gtttgaaaaa tgggtattgg
    13621 acggagaaac agctgttcag gcacaagaac aagattcaag cggaatggtg cgccagatcg
    13681 ttgccgacac acccggtgca atcagctatg ttgctttttc ttatgtttcc gatgatgtag
    13741 atacgttgaa tgttaacggt gtagaaccga cagatgaaaa tgtgacaacg aacaaatgga
    13801 cgatttgggc atatgagcat atgtacacaa gagatcatcc aaaaaaactg acaaaagaat
    13861 ttttagacta tatcttatct gatgaagtac aagataacat cgttggagaa ctaggttata
    13921 tccctgtttc tcatatgaaa gtcgaacgtg attggcaagg aaatatcgtt aaagagtaat
    13981 ctttacacaa aatttacaaa cttagctaaa cttttacatg ttattaaaac caaattgatg
    14041 ttcaatttgt acactgagtg agtatgttaa acctgattaa gggggagtca tctgtggaag
    14101 atgtgaaaaa agtattatta acaaaatcaa aacgttcaaa aatggaacag cgtggaaaat
    14161 tcatcagttt tttatgtatc gcgctgatcg tgttggttgt attatctatt ttttattttg
    14221 tagctagtaa aggattggcc acctttttta ctgataagat caatgtattt gattttctgt
    14281 ttggtacaga ttggaatcca agccaaatcg gagcaaatgg gaaaccaatg gtaggggcat
    14341 tgcctatgat cacaggttcg tttattgtga cctttttatc tgctatcgtt gccacgccat
    14401 ttgccattgg tgcagcagta ttcatgacgg agatctctcc taaaaacgga acgaagatct
    14461 tacagccggt catcgagctg cttgtaggta ttccttctgt cgtttatggt tttatcggtt
    14521 tatcagttat cgtgcctttt gtcagaagta tttttggcgg gactgggttt ggtatcttag
    14581 ctggtacatt tgtgttgttc gttatgatct tgccgactgt cacaacgatg actgttgatg
    14641 cattaaaggc agtgccacgg cactatcggg aagcttcatt agcgcttggt gccacaagat
    14701 ggcaaacaat ctacaaagtc gtgctacgag cagccgtgcc cgggatttta acagcagtcg
    14761 tttttggtat ggctcgtgcg tttggtgaag ccttagctat ccaaatggtc atcggtaatg
    14821 cagcattgat gccaacaggt cttactactc ctgcttctac attgacgtcc atcttaacaa
    14881 tgggaatcgg aaatacaatc atgggaacag ttgaaaataa tgtgttatgg tcattagcgc
    14941 tgatcttgct attgatgtca ttattcttta atatcgtgat ccgtatgatt gggaagaaag
    15001 gagagctgaa ataatgaacg caaaacgctc agataaaatt gctacaggtg ttttatatat
    15061 agtttcgggg atcatcgtat tgattttagc agctttacta ctttatatct tagttcgggg
    15121 aataccgcac atttcatggt ccttcttgac cgaaccttcc aaagcctacc aagaaggcgg
    15181 tggaatcggg atccagctat ttaactcgat ttatttgctt ttgatcacga tgatcatcag
    15241 ttttcctatt tctttaggtg cgggtatcta tctttcagaa tatgctggaa aaaactggct
    15301 aacagatgtg attcgcacgt cgattgaaat cttgagctcg ttgccttctg tcgttgtcgg
    15361 tttattcggt ttcttggttt tcgttattca attccagtat ggtttctcga ttatttctgg
    15421 tgcattggct ttgactgtat tcaacttgcc tcttttgact agaacagttg aggagtcttt
    15481 acaagcggtc cattacacac aacgagaagc aggattggcc ttaggtttgt cacgctggga
    15541 aacagtgata aaagtggtgg ttccagaagc gacaccaggg attttgactg gtgtgatcct
    15601 aagttctgga agaatttttg gtgaagcagc tgcattgatc tatacggctg gacaaagtgc
    15661 acctgcgtta gattttacga actggaatcc attaagtgtt tcaagcccga tcagcatttt
    15721 ccgtcaagct gaaacattag ctgtccatat ttggaagatc aatacagaag gcacgatgcc
    15781 tgatggtgtt tctgtttcgg ctggcgcttc tgccgtgttg attattgctg ttttactgtt
    15841 caacttcggc gctcgtgcga ttggcaaacg attataccga aaaatgactt cagcctaagg
    15901 agaagaagca aaatgaaaaa atataactgg aatgaaacaa atgtcatcac catgccaaaa
    15961 gagaaagaag tagcactata tacggatgat ctacatgtct ggtatggcga caatgaagca
    16021 atcaaaggag tggatctgga atttgaaaaa aataagatca ctgcactgat tggtccttcc
    16081 ggctgcggaa aatctacgta tcttcgttcg ttgaatcgaa tgaatgatgg gatcgctaat
    16141 acgaaaatca ctgggaaaat catgtatcaa ggcgtagata ttaatgcgcc tcaagtggat
    16201 gtttatgaga tgcgcaaacg gattggcatg gtttttcaac gtcctaatcc gttcagcaag
    16261 tcgatttatg acaatattac atttgctttg aaacagcacg gcgaaaaaaa caaaaagaaa
    16321 ttagatgaaa tcgttgaaac aagcttgaaa caagctgcct tgtgggacca agtaaaagac
    16381 agcttggaca aaagtgcctt ggctttatct ggcgggcaac agcaacgttt atgtattgct
    16441 cgtgcgatcg cgatgaaacc tgatatcttg ctgctagacg agccggcaag tgctttggac
    16501 cctatttcga caggaacggt tgaagaaaca cttgtaaact tgaaagaaga ctatacgatc
    16561 atcatcgtga cgcataacat gcaacaagca gctcgtatca gcgactatac tgcctttttc
    16621 tatttaggaa aagtaatgga atatgacaaa actaacaaga tatttaccag accaaaaatt
    16681 caggcaaccg aagattatgt gtccggtcat tttggttaag acggaggcct caacatgaca
    16741 aaagagatta tttcatctaa agatcttcat ctgtattatg gaaagaaaga agcactaaaa
    16801 gggatcgatc taagcttcaa tcaaggggaa atcactgcga tgatcggtcc ttctggttgc
    16861 ggaaaatcca cttatctgcg ttctttgaac cggatgaatg acctgattcc gaatgtaacg
    16921 atcaccggaa gtgttttata taaagacaaa gacatctatg gtccaaaaac agatgttgtt
    16981 gaactaagaa aagaaatcgg catggtattc cagcagccaa acccatttcc tttttctatc
    17041 tatgaaaatg tcatctatgg attgaaatta aaaggggaaa aagataaaaa agtccttgat
    17101 caagtagttg aagaaagcct gaaggcagcg tctgtttggg atgatgtgaa agacaaattg
    17161 cataaaagtg ctttatcttt atccggtggg cagcagcagc gtgtatgtat cgcacgtgtt
    17221 ttggcagtta atccggaaat catcttattg gatgaaccaa caagtgcgct tgatcctgtt
    17281 tctactggga aaatcgaaag tatgctgtta gaattaagag agcggtatac gatgatcatc
    17341 gtgacccata atatgtctca agcttcacgt atttcagata agacggcatt ctttttagat
    17401 ggtcacttga tcgaatacaa tgatacgaag aagatattca tgaatccaga aaaacaagag
    17461 acagaagatt atatttcagg tcgatttgga taaggaggaa acggaatgct acgtacacaa
    17521 tttgaagaag aattgctgaa tctccataat cagttctacg aaatggggat gatggtgagc
    17581 agcgccgtgc acaagtcagt tcgtgcatac atcaaacacg ataaaatctt agcccaagaa
    17641 gttatcgaaa acgatataaa tatcaataac atggaaacac gcttggagaa gaaaagtttt
    17701 gaaatgatcg cattgcaaca gccagttaca actgatttgc gtatgatcat tacggtcatg
    17761 aaagctagct cggatttaga acgtatggcc gatcatgcgg tgtctgttgc taaatccacg
    17821 attcgcgtaa aagggcagac gagaataccg gaaatcgaaa aagagatctc cgatatgtcc
    17881 gattatgtga aaaaaatggt ggacaacgtc ctagttgctt atgtcaaaac agacgaaaaa
    17941 gacgcacgta cgatcgccaa tatggatcaa agagtcaatg actatttcaa tcgaatctat
    18001 aatgcaacaa tcaaaaatat gcaggaaaat cctgagacag tcatcagtgg aacagactac
    18061 ttgaatgttg ccagttatct agaacggatc ggagactatg tgacaaacat ctgtgaatgg
    18121 atcgtctact tggctacagg gaaaatcact gagttaaata cgaaccacaa tgagattttc
    18181 taaactgaag ctgtgattat gccgtttgtt taggaaaaga ggttgtgaaa tcagcggttg
    18241 tgaaaaagct gttctgcatc aagtaataag aacagtcaaa caaaaaatag ctcctcatat
    18301 ttttcgtttg gctggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattcggga
    18361 aagggtttgt gacaaaacat gtcacaaacc cttttcatca tttcatagaa aggataaaga
    18421 atgcaaaaaa aaattagcat catcactata ttttttattt gtgtgcttac ttcatgggtt
    18481 attcagcatc aagccgcttt atatgaagta cctgtcatcg agatagaaaa agtacgaata
    18541 aaagaggatc atcaattgat tacaggaaaa ctgatgaaca cggatcaaaa aggagaatcg
    18601 atttatttat cggttcctta tcaaaaaaat caaatagaaa cagcaaatat aaaagcgaga
    18661 caaatgtatc tagtccaaat gcatggagat gaatggcagc tagtagcaaa aaaaaatgat
    18721 ggctggcttt tcttcttcac aagtatcttc attggcgcca tgttgcttat tggtggaaaa
    18781 cagggtctat ttgctttagg aagtctcatc atcaatatcg ttttactttt acttttgttg
    18841 ctcatcttta ctaaaacaga agggatttct ctattaaata tatctatcct gttcgtcgtt
    18901 atagggatga ttattagcat attggcttta gatggatgga atagaagcag tataatcaaa
    18961 atttcagctg cagttacgag tgttttttta gcatttttta tttgtctttt gacaatgaat
    19021 cattggaaag accaaggact gcgctatgag gaactgaatt atctgacacg accgtatcgt
    19081 tctgtttttc tttctagcgt actgattgga acagctggag gaacactcga taccgttatc
    19141 acagtcattg cgacattaga agaattgact aagcgacaac cagaaataac agcgaaacag
    19201 ttatggcttt caggaaggca agtgggtcga gatgtgattg gctcgatgac caatatcctt
    19261 ttgttcgtct atatcagtgg gtcagttcca tcgcttcttt tatatttagg aaatcaatgg
    19321 tcgttcaaag aaacgataga acagcattta tcattggaat ttttacgcgt cattgctggc
    19381 agcttaggaa tagtattatc gatccctatc tcggtcctct tttttctgac tgtaaggagg
    19441 ctgaaacgat gaatgtactt attatacttg ttgggatttt agcaatcagc gtgctactta
    19501 ttggcggtac gcaaggtatg tatattcttt taggcttatt catcaattta gggatctttt
    19561 tcctgttgtt attcggttat catcagaagt ggccgatact ggcattgtcg gtcataggat
    19621 ttttattgat tgctgtggtt atcttatttt ttatcaacgg ttataatcta aaaatgcgtg
    19681 cagcttttgt gagtatactg atctttttat tttgtttcct actattgctt cctatcactg
    19741 attttttagc aatccaaggt tttacatcgg tagaactaga agaattatct ggcttagata
    19801 agactttagc gattgatttt agattactta cgagatcgtt gcttctgatt agtcttagtg
    19861 gtgctgtttt agatgcgagt gtagcaattt ctagtgggac attcgaagta tatcaggcaa
    19921 atccgcaatt atctttttac caattgcgcc attctagttt cgctattgct aaaaaaatat
    19981 tggcgtcaac agtaatgaca cttctttttg cattcatggg aagttcttta gcgttgatcc
    20041 tctggtttat tgatttagac attccttttc agcaagtgat caatgaaaaa tcatttgttc
    20101 tagaatatac gatggctatt cttaccacat tgtccgcact acttgttctt cctttaacct
    20161 gcgtgactac agcatatctc tttacaaaaa agaaagaaca actcaagatg gagtaaaagt
    20221 tacaacttaa gtcctatgac atttttactg aatcagtgca taatatggat gtaaggaaaa
    20281 gcttgcaaac aattttgctt aaggaggcaa tttccatgaa agaaagagaa cgtattttag
    20341 atttagtgaa aaaaggaatc ctttcgactg aagaaggctt agatttatta gaaagcatgg
    20401 caactgaaaa agacgaaaag caaatcaaaa aagaagcaga caaggtaact gctagtcata
    20461 aagaaaaaga tcaggcaagt caattgatcg ataaacttga aaacggcgaa gaagaaattt
    20521 ctgagccagt tgatccaaag gaaagagaaa gacaagatca agaaaactta gaaaagatct
    20581 tagatgaact agcaactgaa gcgaataaaa cttcggcgcg tctagatgaa gtaaatgctc
    20641 aattcgctga caataaggca gcacgcaatg agaaacagga agcattgatg cagctgaata
    20701 caaaagaaga attaggcgag ttgacagaag aagaattagc acagcgtcag acgttagaag
    20761 cagaaatcaa agaattagaa gctagtggcg atacactgtt agaagaacaa ttgaaattag
    20821 aagcagaatt aaaagatatc cgtaagaatc aatggtctga gaaaaaagaa acgtttactg
    20881 ataagtttga attgccagat gattggaaag atcaagcaac agatacactg aatcaagtag
    20941 gcgaaaaaat gtcagaagct ggatcacagt taggcaaatt cttgaagaaa acattccaaa
    21001 ctgtttcaga aacagtgaat gacaacatgg aatggaaaga tgtcagctta cgtgtccctg
    21061 ggattgcgac aacgaaattt gagcatgaat tttactatga agctcctgca gcaagtatcc
    21121 ttgatatcaa ggcagcaaac ggaaacgtga cgttaaaaac gtgggacagc aacgatgtga
    21181 aagttgaagc taagatcaaa ctttacggaa aaatgggtgc tgaaccattt gaagcattct
    21241 ctgaacggag ccagattgag gttaatgaag atcatatctc cttccaaatt ccaaataaac
    21301 gtgtgcgtgc agaccttgtt ttctacttac ctaaacgagt atacgaccac gcagctatta
    21361 aacttctaaa tggcaacatc atgattgaaa cattggaagc taaagatatt tatacaaaat
    21421 ctacgaatgg taacatcatc gtggaccaat taactgcaac gatgctggaa gtagaaggtg
    21481 taaacggcaa tatcgatatt cgaaacggga acatcttaga ttctattatc gaaacagtaa
    21541 atggtacagt gacatttggt gcgacagcag aaaacctttc tgtgtctttg gtaaatggtg
    21601 atgtacgtct tacgattaaa gaagataatt tgaaaaaagt agaagcaagt tcagtgaacg
    21661 gaaatgtcaa agtcgcttta ccagatggca tcggtttgga aggacatgct aaaacaagtc
    21721 ttggaagtat caacagccgt ctgtccaatt acgaagtagt tcgtgagaaa aaagaacgga
    21781 caaaccaaat gttgcaattc cgtcgtgttt cagatggtga aatcgcgcaa gtgcaacttt
    21841 caacgacaac aggaagtatc tatttaaaag atacagataa ataaagaggt tgtgaaagag
    21901 atgttcagcc ttgagcaata agaagcgatt ttgtaaaata gcttccccta ttttacaaaa
    21961 ttgtgcctta ttgccgatag tctatctctt gaacaccgtt taccaagaga ttatgaacct
    22021 gccaattatc gccgagtcac aaggaataat ttcagaaaaa gctcgtcacg ttttatgaat
    22081 atggtaattt gtgaccgaga attttgcaga aagacaccga ttaagaagaa ggttgtcaca
    22141 acgattttct tcatttaaaa tagtagaaca gaggtgagac atgatgaaaa aaaagttgac
    22201 aaaatctaat aaaaatgttg ttttgacagg gactttagca ggaataggag aatacatcgg
    22261 gattgatccg actgttatcc gtgtgcttta tgtattcctg agcttagttg cttttggaag
    22321 tccaattatt ttatacattt tattagcttt gattatccca tctgcgcgtt ctgcacgaac
    22381 aaactatgga cataacaatg catattatga aaaaaacaat tatcgtgaac aaactcgtga
    22441 acaaacaaaa aaacgcaaag aagcggaaaa aatcgatgat gacgaatgga gtgactttta
    22501 atgtcgtact ttcaacgtat tttagttaat acgttaacat tcatatcatt ggctgttctt
    22561 tttcctgcga tggtccatgt gagcagtatt tggacggccg ttcttgccag cttcgtattg
    22621 tctgtgttga atatgtttgt caaaccgatt ttgacgatac tgtccctgcc ctttacatta
    22681 gtcacattgg gattgtttac gtttgttatc aatgcagcga ttcttaaaat gacctccttt
    22741 tttgtgggag cagaacattt tagtttttct tcattcggtg cagcgttgtt agtagctatc
    22801 ttgatgtcgc ttgtcaatgt aattgtcagc gaacataaca gtagaaaata aaagaaagct
    22861 gtgccgaact atcggcgcag tttttttatt tagaatcagg aatgcgtttt ctattacatt
    22921 ggtaattaaa taaaaacgtt ttttttaatt ggtagtactt ttggctagac aatgataaaa
    22981 tgatagagaa cactctaata gaaatgaggt agcatgaatg tcagaagttg taaaagttag
    23041 ccaattagtg aaaaaattat ctttggaaat cgtgacaggc gacgagaaaa gcttagaccg
    23101 tgtcatcgta actggggaca tctcacgccc aggactagag ttgactggtt attttaatta
    23161 ttattcccat aatcgtttac agctatttgg cagtaaagaa atcagttttg cggaacggat
    23221 gatcccagaa gagcgtttga tcatcatgag aagattatgt agtgaagata tgcccgcatt
    23281 tattatttca agaggattac cagctccaga agaaatgatc caggcagcaa atgaaaatgg
    23341 gatagctgta ctgcgttctc ccatctcaac ttctcgttta ttaggagaat tatccagtta
    23401 tttagatggt cgccttgccc ctcgaacaag tgtccacggt gttttagtgg atgtttacgg
    23461 attaggtgta ctgattcaag gtgacagtgg gataggaaaa agtgaaacag cgttggagtt
    23521 gatcaaacgc ggccatcgat tgatcgcaga cgatcgagta gatgtttatc agcaagatga
    23581 attaacggtg atcggagaac cgcctaaaat cctggaacat ctgattgaaa tccgaggaat
    23641 cgggatcatt gatgtaatga atctttttgg tgcgagtgcg gtacgtggct ctatgcaagt
    23701 ccagttggct gtttatctgg aagcatgggc aaaagataag aaatacgacc gtctgggctc
    23761 tgaagacacg accgttgaaa tcgcggaagt gggcattcca caaataaaaa tccctgtcaa
    23821 aacaggacga aacgttgcaa tcatcattga agtagccgcg atgaatttcc gtgcacgtac
    23881 aatgggcttt gacgcaacga aaacatttga agaaagatta agtcgcttga ttgaagaaaa
    23941 ctcaggcaac gactagcaag gagtgacaat atgttagcac aaatcaatcg agttgctttt
    24001 gagcttgtag ggattcctat ctactggtat gcattgatca tcgtatcagg tatcattatc
    24061 gctatgtggc ttagcagcag agaggctgtt cgggtaggat taaaagcaga tgatgtgaca
    24121 gattttatgt tgtggggatt gcctatctcc attattggcg ctcgcttata ttatgtttta
    24181 tttgatcttc cccagtatat agctgatcca atccaaatat tcaatatccg ttcaggaggc
    24241 ttagctattt atggcggact gattgcaggc gcgatcacgc tgtatttttt tactaaatat
    24301 cattttattt ctttgtggac ttttttagat atcgctgcac caagtgtatt attagcacag
    24361 gcaatcggtc gttggggaaa tttcatgaac cacgaagcat atggaccaga cacgacgcga
    24421 agcttcttag aaagtctgca tttaccgcaa ttcatcattg ataatatgta tatcgaaggg
    24481 atctatcgtc aaccaacatt tctttatgaa tcactttgga gtttgattgg atttattttg
    24541 cttttgtttt tacgaaagaa accacacctt ttgaaaaaag gcgaagtcct tttatgttat
    24601 ctaatgtggt atagtttcgg gcgtttcttt attgaaggat tacgtacaga cagtctttac
    24661 tttttaggtg tgatccgtat ttctcaatta ttatctgctg tgttatttgt cggtacattg
    24721 attctattta tttggcgcag aaaaaaagga aatttaccgg attatgaccg ctcattagga
    24781 aaaaatcaac aaattatctg atttttagag cgttaaaaat gaaaaaaatt tcaggagtgg
    24841 taaaatgaat tatagagtag cggttttagg accaggctct tggggtactg ctttggcaca
    24901 agtcctcgcc gaaaacggac atgacgttcg gatttgggga catcgtgctt cacaagtaga
    24961 agaaatcaac acccaacaca cgaatcaacg ttatttacca gatcattatc tacccgattc
    25021 catccaagca ttcaccaata tggaagatgc agtaaaagac gcagatgcgg tgctattcgt
    25081 cattccaacg aaagcaatac gttctgttgc aaaagaacta gtgcctaaat tgaatacaaa
    25141 gccagtgatc atccatgcaa gtaaaggatt agaacaggga acacataaaa ggatctctga
    25201 catcttgtta gaggaaatcc cagcagaaaa aagaaaagct gttgttgttc tttctggacc
    25261 aagccatgca gaagaagtag caaaacatga catcactacg atcacagctg caagtacaga
    25321 tgcaaaagca gcaaaatatg tacaagagct gttcatgaat aattattttc ggatctatac
    25381 caatcctgat gtgatcggtg tagagatggg ggcagctttg aaaaatatta ttgctattgg
    25441 tgctggcgct attcacggct tagggtttgg cgataatgca aaggcagcaa tcatgacgcg
    25501 cggattagct gagatcagcc gtttaggtgt agcaatgggt gccaacccgc tgacctttat
    25561 cggattaagc ggaataggcg atctagtcgt tacttgcaca agtgttcatt ctaggaactg
    25621 gcgcgcaggc aacttgttag gacaagggca gcccctagaa gaggtgctag ccaatatggg
    25681 aatggtcgtt gaaggtgtga atactaccca agcagcgatg gaactagccc aaatgatgga
    25741 aatcgaaatg cccatcactc aaacaatcta tgaagtgctg tatgaacaaa aagaaatcaa
    25801 aacagccgca aaagaaatca tgttacgtga tggaaaaaaa gaaaatgaat tctcattaac
    25861 aattttttga aggagcgaat gtatatgaaa gttaaaaaag cagttattcc agcagcagga
    25921 ttaggaacaa gatttttacc agcaacaaaa gctatggcaa aggaaatgtt gccaattgtt
    25981 gataaaccaa caatccagtt catcgttgaa gaagcgttga aatcaggtat cgaagacatc
    26041 ttgatcgtta caggaaaagc taaacgacca atcgaagatc actttgattc aaatattgaa
    26101 ttagaaacaa acctaagtga aaaaggaaag accgaattat tgaaattagt agaagaaacg
    26161 acagatgtca acttgcattt catccgtcaa tctcatccaa agggtttagg acatgccgtt
    26221 cttcaagcaa gagcctttat tggaaatgaa ccatttgtcg ttatgctagg cgatgatata
    26281 atggaagacg aagtaccatt gacacagcaa ttaatcgatg attacgaacg tactcatgct
    26341 tctacgatcg ccgttatgca agttcctcat gatgaaactt caaaatacgg aattatcaat
    26401 ccaggagaag tattggaaga tgggttatac aatgtcaaaa actttgttga aaaaccagat
    26461 ccaagcaaag caccaagtga tttagctatt attggacgtt acttattgac tccagaaatt
    26521 tttgatgtat tgtccgagca acaaccagga gccggaaatg aaatccaact aactgacgca
    26581 atcgatacat tgaataaaac acaacgcgta tttgcccgta aattcacagg taaacgttac
    26641 gatgtcggtg acaaatttgg ttttatgaaa acaagtattg aatatggtct aacacatcca
    26701 gaagtaggtg cacctttacg tgagtacatc ctgtctttag gtaaaacatt agaagcagaa
    26761 ggaagcaaga tcgatcataa tccaaaagga ataacagaaa tcaaagaaga aaagaaaaaa
    26821 taaccctttt ttctaacgag cttcagctgc taattgaata aaaagtagtt gaagctcgtt
    26881 tttatttgtt taaaaaacac gaaaatgcta tccttataga aaagagagac aagaagggag
    26941 tttgatcatg tcaggaggag aaattgcagg attgatcgca gcaatcgcct ttgcgatact
    27001 tgtcgttttt atcgttcgtg tgttgttgca agtttctaaa actttagaaa aagtagatca
    27061 gactgtagca gaagcaaata cgacgatcga aatcgtcaca aaagacgtag atcttttgtc
    27121 tagacaagta gaaggcttac tggtcaaaag taatgaactg ctcttggata tcaatggaaa
    27181 agtcgcaacg atcgatccat tgttcacagc agttgctgat ttaagtgaaa gtgtatcaga
    27241 acttaatcat tctagtaaaa acatcgcgac gaaagtcggc ggaatcggta aaacgacagc
    27301 taaagcgacg gttgccggaa aagtcggtgg gacagcgatg aaatttttta aaaacaaaaa
    27361 acatactgaa acgacaggag gaaaataaaa atggctaaaa aaggtggatt tttattaggt
    27421 gcagtagtcg gaggcacagc cgcagcaatc gccgcgttat tgttagcacc aaaatctggt
    27481 aaagaacttc gggatgaatt agctgcccaa gcggatgatt tgatggatat ggcttcagat
    27541 tacacagata tggcgaaaga aaaatcgact gagttaacag gtattgcaaa agaaaaagcg
    27601 caagatcttt ctcaacaagc aggtgatcta gcaggtagtg tcaaacaaaa agtaagtgaa
    27661 tcagcagatg aagcaggaag tgaatctgac gatatcttgt taaatcttaa agaacaatca
    27721 gctgacttat ctaatcgttt caaaaaaacg gcagaagata tgaaaaaacc tgcagatgaa
    27781 ttaggaagta ttgctaaaga tacttcagaa gatatcatga ttgatgtaaa agacgctgct
    27841 ggtaaagcag gagatactgt caatgaagga atgtcagaag caaaaccagt tgcagatgac
    27901 tcaaaagaaa tgatgaaaat ggcaaaagaa gatgcgaaag aagctaaaaa agaaataaaa
    27961 gatacaatga cagattcatc tacaaatcac taaaatcaca aatttataga ataaaaaggt
    28021 gggagcggac tagctctcac cttttttact cttatttttc taaaacaagt aattcttttg
    28081 gtgtagtcgt gaatggcaca cagccatcgc ttgtcacata tacacagtct tcgatacgta
    28141 cccctacttt ttcaggaata tagattcctg gttcaagaga gaagcacatc ccttcttcga
    28201 ttactaagtc atttccttct acaagggaag ggaattcgtg gacggttgtt ccgatcccgt
    28261 gacctaaacg gtgattgaaa tattcgccgt agccatagct gttgatcacg tttcgtgcga
    28321 tttgatcaag ttcacctgct gtcacacctg gacggacggc ttcttgtgcg gcaagctgtg
    28381 cttcaagagt gatgttgtag attttttctt ggaagtcggt tggtttttga taagcaattg
    28441 tacgagtagc atcactgcaa tagccattcc aaacaacgcc aagatcgaat aacacaagtt
    28501 cattcggtga aatcttcgta tttcctggtg taccatgagg gcttgcagca tttgctcctg
    28561 caaggactaa tgtgtcgaaa gacatttgtg aaacaccttg ttttttcaat tggtattcaa
    28621 tttctgcaat gatcgcttgt tcagtcacgc cttctttcac tgctttaaag ccaatctcaa
    28681 atgcaacatc ggcccattta ccagcttcga tcagtttgtc tttttcctct agagttttga
    28741 tcagttgtag acgttggatg atggaagtta gatcagtgta agaatccgtt cccgtcaaga
    28801 tatcagagat agcatcgaag cgctcgacag tcaaggcgtt cttttcaatc cctacattgc
    28861 caggctgacc atagcgagca cgaagttctt ttccgatgat ctcccaaggg ttttcgctgt
    28921 ctaaatagcc acggacatca tacgcccaac tactgttttg agcatcttca acttccaaag
    28981 caggagtgaa aaggaaagga tcttgttcaa gagggataaa aagagctagt acacgctcat
    29041 gaggatcact ttcgtatccg gaaaagtaag cgatgtggcc agggtcgctg atataagcca
    29101 attcgacttg gtgttgtgcc atccattgac gtagttcatt gatttttacg ttattcattt
    29161 ggcaacctac tttctaaatt aattttgtat tcaataattg taccactgat ttattaaaag
    29221 accaacgttc taaaaactga aaaaagtaaa aattttcatg aaaacagaga taaaacggtt
    29281 gcaattcttg agattatttg atattctatt ttagaaatgt aagacgtatt ttcagaaaac
    29341 gtataaaata tgaataggag aacacaaatg gaaaagcaaa caatcactat ctatgatgta
    29401 gcgagagagg caaatgtttc gatggctact gtttcacgtg tcgtaaatgg caatccaaac
    29461 gttaaaccag caacaagaaa aaaagtcttg gaagttatcg atcgcttgga ttatcgtcca
    29521 aacgcagttg cgcgtggctt ggcaagtaag aaaacaacaa cagttggtgt aatcatccct
    29581 gatgtcagca atatgttctt tgcttcttta gcacgtggta tcgatgatgt ggcaacaatg
    29641 tacaaataca atattatctt agcaaattca gatggcaacg accaaaaaga agtcaatgta
    29701 ttgaataact tactggctaa gcaagtggac ggcgtgatct tcatgggtca ccatatcaca
    29761 gacgaaatcc gtggagaatt ttcacgttca aaaactcctg ttgtattagc tggctctatt
    29821 gatccagatg aacaagtagg tagcgtaaac attgattatg tatccgcaac aaaagatgca
    29881 gtaaacaaat tggcaaaaag cggcaataaa aaaatcgctt ttgtcagtgg tgcgttgatc
    29941 gatcccatca atggacaaaa tcgtctgaaa ggctataaag aagcattaag cgaaaataac
    30001 ctaccatata gcgaaggatt gatctttgaa gcacaataca acttcaaaga tggtctatct
    30061 ctagtcgatc ggatccataa tagcggtgca acagctgtct atgtttcaga tgatgaatta
    30121 gccatcggta ttttggatgg tttattagac cgtggcgtga aagtaccgga agaattcgaa
    30181 atcatcacaa gtaacaattc actactgaca gaagtagctc gtcctcgttt aacaagtatc
    30241 acacaaccat tatacgattt aggggcggta tctatgcgcc tattgacgaa aatgatgaat
    30301 aaagaagaag tcgaagaaaa aacgattatc ttgccttatg gaatcgaaga aaaaggatct
    30361 acaaaataat aaaaggacaa ccattcagag gttgtgtgcc tgccgtttaa ctctgcgtta
    30421 caaggagcaa tttcaaaaaa cagcatctca tgtttgatga atattgtgac tcgtgattcg
    30481 gagttggcag gagaacaccg tttatttggg aaaaggggtt atgacaaaat tttgttataa
    30541 tccttttttt gtgtcttaaa aaagagagaa agtggttgaa attacattta tcgtatataa
    30601 aacaatccgt gtatagatgc tgcattttgt tcaccaccat caaaaaaata agggactgtg
    30661 acaatactat tgtcacaatc ccttattact gtgtattcta ttaaatcaat catctttctc
    30721 atctgattct ttagttgctt gattcgctct tgcataagct gaagcggtgt tccagtaatc
    30781 tgtatagttt tcatcggtac cgccaacgcc aaatttaaag gcgcttttct tcggtccatt
    30841 tttcgcccat agcgattcaa ctgtttttcc tggtgtttgg atggattttt tattgacagt
    30901 aacttttcca ggtgtttgac cagtaaatcc agatactttt ttcttcatca catcagatga
    30961 taattcgaat ttctcatcag tgccaaaaat gtcaggtttt gcttggtaag cttgattgat
    31021 cagattggca agataattag ccgaacgtat ccttgctttg ctgtccattc cagtattgtc
    31081 atcgtgccct gcccagctac tgatggtaat agacggagta gagacaatta gccatgaatc
    31141 tcggtattca ttggtagaac cagtttttcc gacccaatct gcttttccta gatttccgtt
    31201 taagcttgag atggcatctt tgaatggtgt agtgattttt gcattgatca cactacgcat
    31261 catgtcattc atgatcgatg ccgttgcttc cgaatagatc cggacagagt tgctttcgtg
    31321 tttataaatt acatttcctg cgttatctgt gatggaatcg atgatatagc cttcttcata
    31381 gacgccgccg tttgctaaag cctgaaatcc gttggtctgt tgaagcgtcg tgacatctac
    31441 tgtacctaaa ggagcagatt caacgcccca attgtcattt gccgggtagt tcatctttgc
    31501 caagtagtgc tcgtaagcat attgtttcga tccaccggaa ttcaacacat cttgatataa
    31561 gtgataagcc gggatattgt tggaccattc tagtgactca cggacggttt ggaaggtgtt
    31621 cgatccttcg ttagtagcat tgacgatttt ctcgccagca ttttctcctt cttgccaagt
    31681 tgttgcataa tctgatacac gggattctga tccgataagc cccatgtcga ttgcaggtcc
    31741 ataaacaaga actggtttga ttgatgagcc agcttgacgt tctgtatcga aggcatggtt
    31801 attttgattc tgactgtaat tccgtccgcc aacgaatccg tagatgcggc cagttctgtt
    31861 atccatcaat acatttccgg tttctacttg actgccagca ccatcatcta acatgtatcc
    31921 ataattttca acgccatttt gcaatgcagc atagacatct ttatcgattg tcgaatggat
    31981 tgtatatcct ttattttgga tcgtttgctg tgccttttga taataagcgt cactgaccga
    32041 tgaatcagac atatcagcgt tgtctttttc agccaattgt tgtgcaatga ttcttgttgc
    32101 ttcattcatc acagtataat acaagaattc tctgtcagtc tgttctgcga tttgttgtgg
    32161 tagaaagtct tttgtaaggt cataggcttt tgcttcttcg tattgttctt tcgtaatttg
    32221 gccttcacga tacatactga ataaaacaaa atcttttctg gcaaggccgg cagaaagatc
    32281 ttcttttagc gcgcctgtgt tcgtgtatgg actatacgtg atcgggcttt ggggtaatcc
    32341 agcgatataa gcagcttgcg gcagcgtcac atcttttgca tctacaccaa agattccttt
    32401 tgcagcttcc tggactccag caatgttttg tcccttatta ttacgaccaa atggtgagac
    32461 attcaaatac gtagcaatga tctcatcttt tgaaaagtat ttttctactt gtgcagaaag
    32521 aatgatctcg tttgctttac gtttgaatgt tgtttcatct gtaaggatct gctgtttgac
    32581 taattgctgt gtcaaagttg atccaccaga agagccaata ccagtagctt cagatactaa
    32641 tgctcgtaaa acagctttag gaacgtaacc ttggtgttta tcgaagtatt catcctctgt
    32701 gctgataatt gcagttttca ataaaggtga aatcttatca gatgaaatag tcgtgcgcat
    32761 cagatctgtc tggatttttg atatctctgt attatctgca taaacaatct ttgacgtttc
    32821 tgtaatgttt cctagttctg tctgcagtgc ttttttagtc ggcaattgtg tgtcttcgac
    32881 gagataggca aaatagccag cgcctattcc aatacctaga gctgcgaaca tcaaaagaag
    32941 aacagtaata aaaacagtca atgattgaaa aactctaaaa ataatcttag gaacaaacca
    33001 tttttctttt tttcttctat tctgtttttt tcgagtattt ttgcgtggca aaagggtcac
    33061 ctcaactaat ggttttattt cgctaattat agcaaataat agaacaaaaa aacagacaag
    33121 gctgtttttg gcaggaattt cttttttaaa agagttaaaa cgtgagaaaa tgatctttat
    33181 tataaaagag ttcttaagct tttctttaga gaaaatttca gttactatgg agaacagggc
    33241 aaagaaaaat caatcatttt ctttgtatat catcttacca atatatagga attgttagta
    33301 acaattgcct ttcaaggagg aatccatcca tgttattagg ctcacacgtc agcatgagcg
    33361 gaaagaaaat gctgttagga gcagcagaag aagcagccag ttatggatct tcgactttta
    33421 tgatctatac aggcgcccct caaaacacta gaagaaaagc catcgaagag atgaatattg
    33481 atgccggaaa agcgtacatg gcagaaaaag gtctaacaaa tatcgtcgtt catgcccctt
    33541 atatcattaa tttggggaat acgatcaaac cagaaatgtt tccttttgcc gtccaatttc
    33601 tgagagaaga gattgttcgt gcggaagctt taggcgctac tcaaattaca cttcacccag
    33661 gtgctcatgt aggggcagga acagaagccg gaattgctag aattattgaa ggcttgaatg
    33721 aagttttgac aaaagaacag acggcacaaa tcgctttgga gacaatggca ggtaaaggta
    33781 cggaaatcgg tcgaacattt gaggaattag cgcaaatcat cgatggagta aaattgaatg
    33841 aaaaattatc cgttactttt gacacctgtc atacgaatga tgccggttat aatattaaag
    33901 aagacttttc aggtgtaatg gaagagtttg ataagatcat cgggttagat cggcttaaag
    33961 tactccatat caatgattct aaaaatccgc aaggttcgca taaagaccgg cacgccaata
    34021 ttggttttgg aacaatcgga ttcgacacgc tgaataagat cgtccatgat ccaatgtttg
    34081 acaatatttc gaaaatcttg gaaactccct atgtaggcaa agataaaaag aaagcttacg
    34141 caccatatgg aaaagaaatc gccatgttca aagcgcaaac attcgatcca gaagtgtttc
    34201 ctgagttatt agcagaagaa gtgatactct aagattttct atctgacagc agcaaaaaag
    34261 gaccaggcac attgccttgt ccttttttgt ttaagaaaca aaggagtact ttttgcagtc
    34321 tttttcatca tgcatcttct cccccctctt tttaaattta gacaaacaag gtatactggt
    34381 agaaaagtaa aaaaacgaaa agtttcacaa gcaagtggca aaacgtgatt ggaaggacga
    34441 aaagatgctg aacactttaa aacccttgat caaccaacta aaaaagaaac atacatactt
    34501 tattcttggc atagccctat tatttacaat tattcgtttg tatttatata gtaaagcaat
    34561 ctatggaaca gacatgtcgg caacctatga tgaccagctg ctgattcgct atgctcaaaa
    34621 tattttggac ggtaaatggc taggggagta tacgactaca acactttcca aaggaatctc
    34681 ctacagctta tttttagtac ttgcacataa actgcatctt tcttatgcag ttttgttcgg
    34741 catgttcagt atttttgcca gtatattggt tgcactcgcg tttaaaccga ttgtgaagaa
    34801 caagtatctt cttttaagta tctattttta ttttttgttt tctccgatta gttttacaca
    34861 tgaatattcc actcgaacat atcgtaatgc cctcgtgatt ccggctgttg agatcattat
    34921 cgcttgttta ttagctattt attaccgcaa atacggttct agcagaaaat tgctgccatg
    34981 gtttttaggg ctttctgtcg cttttccttt cttttggttc attcgcgaag attctatctg
    35041 gcttcttcct tttactgtag tagctttggg tatcagtttc gtccagatct tgctagggca
    35101 agacttttct ttcaaagaag taaagaagtt aaaaaaacga ctctttcttc tctcgaaacg
    35161 ggaatggctg aaactttcat tgtttttact tcctatagtt ggcttttcca tcgtctatca
    35221 atggatcgaa tataaaaatg agacaacata cggtattgca accatcaatg accgttccag
    35281 tggagaattt ggacgtttga tgaaacaact gatccgaata gatgatgaaa cgaacttgaa
    35341 taaagaaaac agtgagatat gggtctcaca tgctgcacta aataaagcat tagccgcatc
    35401 tcctacattc cataccgttt ctacccgtat tgacgaatta tatacgactc acgcgtggac
    35461 tcaaggtgga aaagtaaaag aattacccgg agatatcatc ttttgggcat tgcgagatgt
    35521 ggtaagcgaa tctggttatt accaaaacaa tgcaaaagaa accaatgatt tttggaaaaa
    35581 agtcaaccaa gaattagaag aagcctatca caagggaaca atcaaaaaga aaaaagagtt
    35641 ctatctgatg ggatcaggag atggaaagac gatgagtgat ctgccaatcg tattcgattt
    35701 ccttcaatca ggatacgctt atgcatggcg atataaagat ttccatcaag gaggaaatta
    35761 tagtttcgga acgcaagaac aagtcaaaca ggctcaagaa ttattaaacc ttccgctaat
    35821 ggagaattgg atcgatagta atgaaccgag aacacctaat ttccaattaa caaaatcagc
    35881 aaaagtcgcg aatgtcatca taaaaattta ccaagttctg gctattcccg tgctgattgc
    35941 ttcaatagca gggatattac ttatgatact aggaaccgta tttgcaaaag ctgatcgtgc
    36001 ctattatagt tcctctttga ttgtggtcac cggattaata ctgacacaat tggtttttct
    36061 tttcggtgtc tcatggtttt gttcttatgc gccagaaaaa aaggactatt ttttaagtgt
    36121 ctacactggt gcaggcgtgc cattaacgca attcatcgta gtaacaagca tgctagtttt
    36181 cagtaaatta aatctgaaaa aagctttatc ttctaaataa aaagaccctt ggtagctaga
    36241 tttttaacct aactatcaag ggtctttgtg tggaaaaatc agtttttatt cgatttcttc
    36301 taccgtttcc tgcaaaagct gttcatattt tttgtttcct ttatataaat caccaatcgt
    36361 ccatgccaac aggagaagga caagaacaat gattacataa gcgatgtgat cagccaatgc
    36421 tagttgagcg gaagtggctt gctcgccgaa gaaactttcg atctgtcctg gtaatcctcg
    36481 taagttcaag taagttagac taatcacaga gaaccagcca agaagtttta cgatgaatga
    36541 atttttaaat cggctaccca tctccgcttg gctgttggtc atcatcagta aaggaatcat
    36601 cgaaaacggc aacgcaaaag ccaagaacac ctgggattca ttcattaagt tattcaaagc
    36661 ggcatgttct tcgatcgttc cttttgtact tgtaaacaaa acacaaatca atacaggaat
    36721 cacagaaagc aatcgtgtga ccagtcttct tagccaaatc gggatacgca tatggatgaa
    36781 tccttccatg atgacttgac cggtcaacgt accagtaata gtagagttct ggccagaagc
    36841 aagaagtgct acagcaaata aagtggacaa gataccagat tttgctacag caatcaaaat
    36901 accattgctc atagctgatg gatcagataa agcttcatac agaccaaaaa aggaaggatc
    36961 ttggacagaa ccggttttga agacagcgac acccatgatc aatagcagtg cattgactag
    37021 aaaagccatt gacaactgga tattagaatc ccaagtagaa aaacgtacag cttgtgctac
    37081 atcttcttcg ttttggtgat cgatcttccg agtttgtgaa actgctgaat gaagatataa
    37141 gttatgcggc atgactgttg caccaataat ccctaaagct ccagtcagcg gagtttcgcc
    37201 gccgatgacc ggtgagtctg agaatgtatg actgttaggg ataaagccag caaataaact
    37261 gtgccaatct gggttagaca agccaacttg atagacaaaa acgaataaaa tgacgaaaat
    37321 taagcagaca accagtgctt cgatcttacg aaacccgact tttgtcagta gtaagagaac
    37381 gaaaacatct aatacagtaa taaagacggc aatcaccaat ggaatatcaa aaagtaagta
    37441 gagggcgatt gctgcaccaa tgacttctgc aatatctgtt gccataatgg ctaattcagt
    37501 caagatccat aaaatgacac ctaatgtctt acttgtgcgt gctcggattg cctgtgccaa
    37561 atccatttgg ctcacgattc ctaattttgc tgccatatac tgtaaaagca tagcaatcag
    37621 actagacatc aaaataactg acatcaataa atattggaaa ttttgtcctc cggtgatcga
    37681 agtagaccag ttacccgggt ccatataccc aactgctact aacgcaccag gtccagagta
    37741 agcaagaagt gttttaaaaa aacttttccc ctttggtaca tcaactgttc catttacttc
    37801 agctaaagat aaaccatttg tatgttcaat aagtcgatgc ttatgaaatt gtttattgga
    37861 ttgattattc aagaaaaaac cacctttcct atttatctac gattacattc tacacctcaa
    37921 aaagaaaaaa ttaaagaaaa agattcggta cgcctaattt ttttcatggc gcgtcttgct
    37981 agatgacgaa agaaattttg ttctcctcta aaaaaaccac tattttttgc atgaaactaa
    38041 agaatacgcc tttctttttt caataaactt tagtaaaata agtcattgag agcaattcgg
    38101 aaatagaaag gagagattta tatgttatca acaggtattg tcgtacttat tgctattatt
    38161 gctttgctaa tcggcgcagt aggtggcttt ttcctagcgc gtaaatatat gaaagactat
    38221 ttagaaaaaa atcctcccgt taatgaggac atgttgcgat caatgatgat gtcaatgggg
    38281 caaaaaccat ctgagaagaa aattcgccag atgatgcagc aaatgaagaa ccaagggaaa
    38341 aaataggttc tgacccaacg actttttacg ggctgatttt ttcagtccgt tttttatttc
    38401 gtaatttgtt agactattca gattattcag caaaaaagac aagcacagac aatcctaggc
    38461 tcttgataaa gacaaaggag gagtaaaatg tcgattttta gaaaattagg ctggtttttt
    38521 cgtcaggaaa agaaacatta tttaattggg gttttctcgc tattcgcagt ggccttggta
    38581 cagttagttc ctccaaaagt gattgggatc atcattgatg agatcgccga taaaaattta
    38641 tcaataaaga tcattttcgg gtggatcttc ctattgatta tcgctgcttt tttacaatat
    38701 ctttttcgtt atatctggcg gacgaatatt tggggaagtg cagctcgatt ggagaaagaa
    38761 cttcggcagc agttattcaa acattttaca gaaatggaca atctctttta ccaaaaatat
    38821 cggactgggg atttgatggc tcacgcaacg aatgatttga atgcgatcca gaatgtagca
    38881 ggagctggta tcttgacctt tgcagattca gtgattacgg gcggaacaac gatcattgcg
    38941 atgattttgt ttattgattg gcgcttaaca ctgattgcgc tgattcctct gcctttgctg
    39001 gccgtcactt cacgagtgct ggggtcaaaa ctccatgatg cttttcgtga ttcgcaagaa
    39061 gctttttcta gtatcaatga taagacacaa gaaagtatca ctgggatcaa agtcattaaa
    39121 acatttggac aagaaaaaga agatctagcg gattttgaag aaaagattga agatgcaatt
    39181 attaaaaaca aacgagtcaa ttttttagac tccttatttg atccattcat taccttgatt
    39241 atcggtctgt cttatgttgc aacaattatt ttaggtggaa agtatgtctt ggaaggcacg
    39301 atctcgatcg ggcagatggt ctcatttatt agttatatcg gtatgcttgt ttggccgatg
    39361 tttgcgattg gtcgattgtt caacgtattg gaaagaggaa atgcttctta tgatcgcgtc
    39421 aatgaacttt tgcatgaaaa gacgcatatc gtggaacaac tgggtgcagt gcagaccccg
    39481 gcaaaaggcg agctgaatgt agctatttcc tcttttaatt atccgaaaga agaatcacca
    39541 gcattagaac aaatcaagtt tgcgatccaa gaaggagaaa cattaggtat tgttgggaaa
    39601 actggtgcgg gaaaaacgac gatcatcaaa ttactgatgc gtgaatacga tgattatcaa
    39661 gggaaaatcc aatttggcgg acgttcgatc aaagaatatt cattgaatgc tttattagga
    39721 tcaatcggct atgtgcctca ggatcacttc ttgttttcga tgaccattcg agataatatc
    39781 cgtttttcta atccaaaact ttcagaagaa gaagtagagc atgcggcttc tttagcattt
    39841 atcaacaatg aaatcaagga attaccaaaa ggttatgata ccttagttgg agaacgtgga
    39901 gtctctttat ctggcggaca gaaacaaagg atttcgattg ctcgagcatt gatcaaaaat
    39961 cctgaattat taatattaga tgatgcactc tctgcagtag atgccaaaac agaagaagct
    40021 atcttgcata atttaaaaga aataagaaaa gagaaaacaa ctatcattgc agctcaccgt
    40081 ttaagtagcg tgatgcatgc aaaagaaatc attgttgtcg atcaagggaa aatcattgaa
    40141 cgaggaaccc atcatgagct gcttgcacta ggcggctggt atgaacaaat gtgggagaaa
    40201 cagcaattag aagcgaagat agaaggaggc gaggcctgat ggatgaaaat tatcaatcgg
    40261 aatggacaaa atcgattcca cttaaagaac agaaaaagat catcaaacga ctgatgaagt
    40321 tcgccaaacc ttttagaaga acgttcatcg ttgctatctt atttgcgttt gctttgtctg
    40381 tgatcaatgt cctattgcca aggatcatcc aaacttttat ggatgatcat ctggctaaac
    40441 agtcggctac gacacaagtt attttgtttt ttgctggtat gtacttattt ggagtcatag
    40501 taaaaagtat catttggttt ttccaatggt atttgtattc catggcctca ttgaagacct
    40561 atcagtatat tagggtaaaa ttatttgaaa agctgcatac actaggtatg cgttattttg
    40621 atcagacacc ggcaggatca accgtttctc gagtgacgaa tgacacagaa acgctatttg
    40681 aattttggta cgtctttttg atggtattga ctgggatctt tgcagtcgtt tcttcattcc
    40741 ttgccatgtt tcaaatcaat ggagaaattg ccttgtggac tttggtgttt ttgccgattt
    40801 taggaatcgt catctggtat taccagaaat tcagttctcg tatttaccga aatatgcggg
    40861 aaaaattaag tcagctgaac acgaaattga atgaatcgat ttccggcatg cgtattatcc
    40921 agcagtttcg ccaagaagct cgtttagcta aagaatttga acaagtgaac aatgagtatt
    40981 tagacacgcg ttatgcgatg atcaaaacga attcgattct gttaagtccg atcatcaatt
    41041 ttctatatgc attggcgatt gcgttggtgt tgactttgtt tgggatcgat gcgttgcatt
    41101 ctcccgtaga agttggactg atctatgcat ttacgaccta tgtccaggga ttttttaatc
    41161 caatgtctca aatgatggac tttttgagca tcttcacaga tgggatggtt gctggtagtc
    41221 gtatcttgaa gattatggat acggaagaat atgcaccaca acaaaaccct ggagcagatc
    41281 aaatgattac ttcaggtaag attgagtttc gcaatgtaag cttttcttat gatggagagc
    41341 acaatgtatt aaacaatatc tcttttgttg ctaatccggg agagaccgtt gcattagtgg
    41401 gacacaccgg aagcggaaaa agctcgatca tcaacgtatt gatgcggttt tacgaatttt
    41461 atgaaggtca gatattgatt gatgatcatg acattcgaga atatccaatg gaagagttac
    41521 gtaagaaaat ggggctggtc ttgcaagatg ctttcttgtt ttatggggat atcgcaggaa
    41581 atattcggat gatgaatcca gctatcacag acgaacagat acgccatgcg gctgagtttg
    41641 ttcaagcaaa caaattcatc gatgaattac caaaaggata tcactcaaaa gtgatcgaac
    41701 atggtgccag ctattctagt ggacaacgac agctgatcac ttttgcgcga acaatcgtga
    41761 ctgatcctaa aattctagta ttggatgaag caacagcaaa tatcgatact gaaacagaag
    41821 gattgatcca agaaggattg gcaaaaatgc gtcaaggacg gacaacgatt gcgattgccc
    41881 atcggctgtc aacgattcga gatgcagaat tgattttagt tttagacaaa ggtcatatag
    41941 tagaacgcgg gaatcatgaa acacttctag cgcaaaatgg tttgtatgca gatatgtaca
    42001 aactgcaaag cagtggtata taaaagatgt tgtgacagta gaattttgac ctaagcacta
    42061 gagtgaaaat aaaacatagc ttctcatatt tttcatctgt gagagttagt gtcgtagtca
    42121 ccagatttaa aaaaagattc gacagctagt cagaagtgcc taatagccac taagaaagcg
    42181 ttgacaaaag catttgcttt cgataatata aagaaaacga tacaacttgg atagtgattg
    42241 cctgatgcaa gctaaacctg atgagtctcc catggtttca tggacggctt tttgggtttt
    42301 tctttttgtt ttggatacag ccctatacat catggaaagg agcaaaacat gctgattgag
    42361 aaagtactga ataacaacgt cgtaataacc agggatgaaa gaaatcagga aatgatcgtg
    42421 atgggaaaag gtttggcatt caaaaagaaa gtgggtgacc cgatttttga ctcatgcatc
    42481 gacaaaaaat ttcagttgac aaatgacggg ctttcacaac agttccaaga attggcacaa
    42541 gcgattcctc ttgagtactt agaaatttct tgtgaaatca ttgaatatac aaaagaggct
    42601 ctacaagtcc agttgaatga ttctgtgtat attgcattga cggatcattt gtatacggct
    42661 atcgaacgtc acaaaatggg aatatttgta ccaaatgtat tgctgtatga tatcaaacaa
    42721 ctatttccta aagagtatgc catcgggaaa aaaacagtga agaaaatctc ggataaatac
    42781 cacatcaagt tatcagaaga tgaagccgga tttatcgcat tgcatttagt caatgcacaa
    42841 ggcattcaaa gccagcaaga caatacctac ctgtttacta taacaataca ggatattcta
    42901 aatatcgtcc gctattacta taagattaca tttgatgaag attcagtcca tttttttcga
    42961 ttcgtcacac acttacgttt ttttctgaca cgcgtcctag atggagaaaa atcagaagtg
    43021 ggagaagttt ctgaggaatt gtttcaggtt attcaaaaaa aatataagga tacgtttggt
    43081 tgcgttgaga aaatagcaga ctttttagcc gagacgttca actaccagat gtccacggac
    43141 gaaaaaatgt atttaacgat ccatactgcg cgtttagtgc atgagaatcg ttgtaaataa
    43201 aaaaaggagg ataaattaac aagaacgtat gaaataaggg tagtgacatt cagttggcta
    43261 aaccttccac taataaattg gagggaatag aaatgggaaa ataccaagaa ttagcgagaa
    43321 aaatcgtaga aaatgtcggc gggaaggaaa atatcaatgg tttgacacat tgtatcacaa
    43381 ggcttcgttt caaattaaaa aacgaagaaa aagcaaatga tgagatcttg aaaaatatgg
    43441 acggtattgt aacggtaatg cgagcgggag ggcagtatca agttgtcatt gggaatcatg
    43501 tgccagtcgt gtttgaagag gttataaaag caggaaacct tacttttgat gaggctgttt
    43561 caacagaaaa aatgagaccg tttgatatgc ttattgatgg aatatcgggc tgtttccagc
    43621 cattccttgc tatcctagct gctggcggga tgattcgtgg actgacagca ttccttgtct
    43681 tcttaggcgc gtttgaccgc ggttctggca catttgttat gtttgataat atcggtgatt
    43741 ctgtattcca atttatgcct gttattatcg gtttaacagc tgcccgtaaa tttaaagtaa
    43801 atgaattcgt agggatgttg cttggcgcag cattaatgaa tcctagcttg tcgctggaag
    43861 ctttgagtgg tgcagcagaa gcaccactaa cgaccatatt ttctggcact atttttgaag
    43921 cacctattta tcaaacagta ttcggcattc cctggattgc gcgtaactac gcttcttctg
    43981 tcattcccat tatctttatt gtgttgttag ctagccaaat tcagaaacca attaaaaagt
    44041 tagtacctga gatgatcgcg aatttcttcg ttccattctt caccgttctt attacaatgc
    44101 ctttaggttt tttactcgtt ggaccagtat tcacattcgc aactgatatt ttaatggctg
    44161 gatttgaaac acttttagca ctttcgccag ttatttatgg agcaatcgta ggcttcttct
    44221 ggcaaattct ggtgatgttt ggtctccatt gggcaatcgt accaatggga ttgatgcaat
    44281 tttctgtgaa tggttggcaa aatattatga cacctgttgc agtagtgtct tttggacaaa
    44341 ctgcagcact taccgcactc tactttaagc ttagaaatcc aaaagacaaa gcaattgcaa
    44401 taccagccat tgtttctggt attgtgggga ttacagagcc agctatttat gggtttacat
    44461 taccacgtaa aaaaatattt atttttacct gtgtcggtgg tgcgttaggg ggcgcgtata
    44521 gtgggttaat gaatttaact tcttggaatc aaggtggact aggcatcttt acaattccca
    44581 actatattag accagatggt gatttaacag atgtgattaa tgtattgatt gggatcgcca
    44641 ttgcgatggt ggtatccttt acgctgactt ttttcttttg gaaggatgaa gcaggtgaaa
    44701 cagatgatat acagaaaaaa tcaggtaaag aaattgtaaa aacaccgata caaggacaaa
    44761 tcgctccact aaatgcagca aaggatgcag cttttgcaca aggaacacta ggtcgaggaa
    44821 tcttgatcta ccctgaaaaa ggagaagtac gtgccccttt tgacggaaca gtcatgaccc
    44881 tgttcccgac gaaacaggca attggaatgg tttctgaaac agggctggaa ttgctgattc
    44941 atgtcggatt ggatactgtg cagttggaag ggaaatattt tgaaagctta gtgcagcaag
    45001 gataaagagt gaaacaaggc gatctgttgc tacgcttcga caaaaagaaa atcgaaaagg
    45061 aaggatacag tttagaaaca cctgtgatcg tcaccaatta cacggattat ttggatattt
    45121 tggaaaacca aggggagacg gtcgggccag acgatgcact tatcaccgca ctgacctgaa
    45181 cggttaggtg aagaatggac taggcttaac tattcttttt attccacttc tttattttca
    45241 aaattgaaag gctctttcat gatggctgag ctgatagggc gtttatcgaa tttttgaccg
    45301 acagaaatta gatggactaa attctgttga taattttctg caaaaacaca actatataac
    45361 acgtctagaa gataacaaat tgatgtattg atcgtaaaat taccaatttt tgaatacagt
    45421 cgctcgcgtg tcgtaagtcg caaagcggct gtactcattg cagatagcgt tgaatcaccg
    45481 atactggtca aagctaaaat tggaacatga ttttgtttca atatttctgc ggtacgaagg
    45541 atcacattgt tttcacccgt ataagaaatc agaatcccgc acatatcact cgtgcaattg
    45601 tacgcttcat agaatgattc accaaaagtg gtgacaatcg tgacatccat tttgatgcgc
    45661 ttcattttca gcatgaaatc ttgagaaatc aaagtattcg agttactggc gaaaatcacg
    45721 attttttttg cttgaagcat tagttgtttg gctttttgca gttggtcatg atgaatgaga
    45781 gaaagtgtat catgaatcgt catctgttct aaagaagcca ttttatttgc gatcgtcata
    45841 ataccgtctg tggtggaaaa aggaagattc gcatctacgt cttgaaaatt cccttcaaga
    45901 taggctgttt cagctaaaaa agcattacga aaactgaccc agccatcaaa acccattttt
    45961 tttgctacac ggatgagtgt agaggggtgg acataacatc gctcagcaat cgcttggatg
    46021 gtcatttcgc caagtgctgg agaatcgctt aagagaaact ggatcacttc attttctgaa
    46081 ggagaaaagg agatttcttt gattttttca cgtaataaca tgtagatacc ttcttcttat
    46141 ttttttacgt aaattataac ttttttgaaa atgatttgta aacggattta tactatataa
    46201 cttaaaatag acattttttg acaagatatt tacaaaaatc cactagaaaa cagtaaattt
    46261 tgtaaatgtt actggaaaga aacagatttc tattgtttgt tttgtttaca aaaaagataa
    46321 tgaaagtgta aaaaagtaaa ggagtgagaa catggcaaaa ggaatcaaaa tcgctacgat
    46381 tggtggaggc agcagttaca cccccgagtt gatggaagga tttatcaaaa gatatgagga
    46441 attaccgata cgagaaatct ggctcgtaga tgtagaagaa ggaaaagaaa agcttgagat
    46501 cgttagtcag atgtctcagc gtatgtggga tgcttctccg tatgaagtca agatctatgc
    46561 aactctggat cgggaagaag cattgaaaga tgcggatttt gttacgactc agtttcgtgt
    46621 cggtcaatta gatgcacgaa tcaaagacga gcggatccct agttattatg ggatgattgg
    46681 gcaagaaaca aatggtgctg gcgggatatt caaagctttt cgtaccgttc cagtcgtttt
    46741 ggcaatcgtt gaggacatga agcgtttatg tccaaatgct tggctgatca atttcgcgaa
    46801 cccaagtggg atggtgacag aagcagttat cagatatggc aaatgggata aagtgattgg
    46861 attatgcaat gtgccagtga tggcgatgat gacagaacca gcaatgcttg gcaagaaaaa
    46921 agaagagctg atctacaagt ttgccggtct aaatcatttc cattggcaca aagtagcaga
    46981 taataaagga aatgaccgca cggcagaatt gattgatcgt ttatacgatg aagagaatgg
    47041 attaccgaaa aatatctttg atatcccgtt tttcaaagaa caattaaaac aaatgaacat
    47101 gatcccttgt ggctaccacc gctattacta tcgtgaagaa gaaatgctgg aacatgcttt
    47161 agaagaatat caaacgattg gtacaagagc gcagcaagtg aaagaaacag aagcagagct
    47221 atttgaatta tacaaagatc cagaattaga ccataaacct gaacaattgc aaaaacgagg
    47281 tggcgcttat tattcagacg ctgcctgtga aacgattgct tcaatctatg gaaataaaga
    47341 aacacagatc gtggtatcta caaagaatga tggagctgtt cctgacctgc cggcagattg
    47401 tgtagtagaa gtcactgcct atgttggcgc acaaggagcg agaaatgtag cgtttggttc
    47461 tcttcctgct gctgaaagag gctggttgca agtaatgaag aatatggaat tgctgacgat
    47521 cgaagcagct gtgactggtg attatgggac agctttacag gcgtttacga tcaatccact
    47581 tatacgatca ggaaagacag caaaacggat catggatgag ttgtttattg cacaccagca
    47641 gtatttaccg caattcaagg agacaatcag ccgattaaaa gaagaaggaa tcgaagttca
    47701 agatgaactg gcaagagaat tatcaggtaa tacacatata atggaggaag tataatggga
    47761 ttcagaaaag attttttatg gggcggtgct acagcagcca atcaatgtga gggcggttat
    47821 gatgaaggtg gacgcggact agcaaacgta gatgttgtgc caatcggaaa agaccgtttt
    47881 cctgttatca ccggaaagaa aaaaatgttt gaatttgatg aggatcattt ttatccagca
    47941 aaaattgcaa tcgatatgta tcatcgttat aaagaagata ttgctttgtt tgcagaaatg
    48001 gggttcaaga cttatcgaat gtccatcgcc tggagtcgta tttttccaat gggggatgag
    48061 aaagagccga atgaagctgg cttgaaattt tatgaggatg cctttaaaga atgccaaaag
    48121 cacggaattg agccacttgt gacgattacg cattttgatt gtccaatgca tttgatcaaa
    48181 gaatatggtg cgtggcgttc cagagaactc gttggttttt atgaaaatct gtgtcgagtc
    48241 atcttcaatc gctataaagg acttgtcaaa tattggctga cattcaatga gatcaatatg
    48301 atccttcatg caccattcat gggcgcaggg ctgtattttg aaaaaggaga aaatgaagaa
    48361 caaatcaaat atcaagcggc acatcatgag ttggttgcaa gtgccatcgc aacgaagatt
    48421 gctcatgaag tagatccgga gaacaaagta ggctgtatgc tggcagcagg agcatattat
    48481 ccatacactt gcaatcctga agatgtttgg gcttctagaa aatcagatcg agaaaattat
    48541 ttcttcattg atgtccagtc acgaggagaa tatcccgctt atgcattgaa agaaatggag
    48601 cgaaatggca ttcagatcga aatggaacca ggggatgccg cattgctgaa agaacatact
    48661 gttgatttta tctctttttc ttattatgct tcccgagttt cgactaccga caacgaactc
    48721 ttggaacaga ctgcaggaaa tatctttgct tctgtcaaaa atccatatct gcaagcaagt
    48781 gaatgggggt ggcaaattga tccattaggc ttgagaataa cgatgaatga tatttatgat
    48841 cgttatcaaa aaccattgtt tattgtagaa aacggcttag gtgcagtcga tacgccagat
    48901 gaaaatggtt atgtggaaga tgattaccga atcgattacc tggcagcgca tatccaggcg
    48961 atgaaggatg cagtagaact agacggagtc gatctattag gctatacgac ttggggatgt
    49021 attgatttag tatcagctgg taccggtgag atgaagaaaa ggtacggatt catttatgtc
    49081 gatcgtgata accaaggaaa tggcacatta gcccgttcaa agaaaaaatc attcaactgg
    49141 tataaaaaag taattgattc aaatggagaa aatctttcga acgattgatc aatgatttat
    49201 aaaagtctat tgtttttcag cggatttttc gagtataata aagctattcg agaaacgcaa
    49261 gcagaacaat agactttttt tcgcattggc tgtaaagtat gcttacttga ttaagtaaag
    49321 gagaatctac atatgtatga tgtaatcgtt attggcgcag gtcctgctgg gatgactgct
    49381 gctttatatg cttcacgttc aaacttatcc gtattgatga tcgaacaagg tgcccctggc
    49441 ggccaaatga acaatacggc agaaatcgaa aattatcctg gattcgattc gatcatgggc
    49501 cccgatctag cttataagat gtatgaaagt gtctcaagat ttgggacaga aaatgcttat
    49561 ggaatcgttc aagatatcaa ggatcacggc agccataaag aagtcatctg tgaagataaa
    49621 agttacgaag gaaaaacagt cattatcgca acaggatgta tccaccgtaa attaggcgta
    49681 cctggcgagg aagaatttgc tggacgaggc gtttcttact gtgcagtctg tgatggtgcg
    49741 tttttccgta acaaacgtct agtagtcatc gggggtgggg actcagcagt agaagaagcc
    49801 atctatttga cacaatttgc ttctgaagta gtgatcgtcc accgtagaga tgaattacgt
    49861 gcacaaaaga tcatccaaga ccgtgcattt gcaaatgaaa aaatttcctt tatgtgggat
    49921 acagtcgttg acgaaatcgt tggaaacgaa atggtagtga caggtgtgaa agcacgtaat
    49981 gtcaaaaccg gcaatgaatt tgatttagca gcagacggag tattcatcta tgttggactg
    50041 gatccattaa ctgcaccttt tagagaagca ggtttgacga acgaagctgg ctggattcca
    50101 acagacaacg aaatgaaaac gaatatgcca ggcgtttttg ccatcggaga tgttcgtgaa
    50161 aaagatttgc gacaaatcac tacagctgtt ggtgacggcg gtattgctgg acaacaagtg
    50221 tataaatact tggagtcttt agaagaaaca acagcataag tttaaaaaga gtagaagaac
    50281 gggttcaatt gttcttttac tctttttttt cgcgtgaaat ttactgaatc tgatttatgc
    50341 gaacacgtcc aacacctttt gataaaagaa tcctttcttg atttaagcgt tttttggtcc
    50401 tcatactttc atatctaata agtgaatgct atacttatta agaaccatat ttcagaaaga
    50461 ggtgttattt tcatgtcttg ggaacaagtg tatgaacaat gggtacatga agaaaattta
    50521 gaagaaaact taaaaaaaca attaaatgat ctgggtgaag atcctgaaaa acttgaagat
    50581 gctttttatg caccgttgga attcggtact gcaggtatgc ggggaatctt aggacctggg
    50641 atcaatcgaa tgaacatcta tacggtaaga caagcaacag aaggactagc acgtttcatg
    50701 gatacacaag gtgaagaaac aaaacgccga ggtgtcgcta tcgcttatga ctcacgccat
    50761 cagtcaccag aatttgcaat ggaagcagcc aaaacactag cacatcacaa tatcccgtct
    50821 tatgtattcg aaagtctgcg tccaacacca gagctttctt ttgcagttcg tcatttgaag
    50881 acgtttgcgg gaatcatggt aaccgcatct cacaacccag cggcttacaa tggatataaa
    50941 gtctacggag aagatggcgg acaaatgcct cctgcagacg cagatgcatt gacgaaatat
    51001 gtccgtgaag tcagcaatcc gctaaaagtc gaagtattat ctgacgaaga agctaaacat
    51061 agcgggttga tcacgatcat cggagaagaa gtggacgctg cttatctaga agaaatcaaa
    51121 gcagtgacga ttgatcgtga attggttgaa acaatgggga aagatttgaa acttgtgtac
    51181 acgccacttc atggaacagg gaaaatgttg ggcgaacgtg cgttgaaaca agctggattc
    51241 gaacaattcg tactagttcc cgaacaagct gtggcagatc cagatttcac tacggtcaaa
    51301 tcgccaaatc cagaagaaca ttcagctttt gaatatgcta tacgacttgg agaaaaagaa
    51361 ggagctgact tgctgattgc aacagatcct gatgcagacc gtttaggcgc agcggttcgt
    51421 ttgccagatg gttcttatga agtattgact gggaaccaaa tcggagcatt gtttgctcaa
    51481 tatattttgg aagcacatca atcaagaggc actttaccag aaaacgcagc ggtattgaaa
    51541 tcaatcgttt ccagcgaatt gcctacagca attgcggaac actacggtgc taccatgttc
    51601 aatgtgttga cagggtttaa attcattgct gaaaaaatcc aacaatttga agaagaccat
    51661 tcacatactt ttatgtttgg attcgaagaa agttatggtt atttagttaa accttttgtt
    51721 cgcgataaag acgcgatcca agcattgctt ttattagcag aagtagcagc ttactataaa
    51781 aaacaaggca aaacgttgta tgacggcttg caaacgattt ttgaaaccta tggctatttc
    51841 gcagaaaaaa cgatttctgt gacaatgagc ggacaagaag gttctgcgaa aatctctgct
    51901 ttgatggaaa aattccgtaa agaagcaccg acagaatttg ccggagcaaa agttattcaa
    51961 acagaagact ttaaagaatt gacgagaaca gaccgtgaag gaaacgtaga aaaactaaca
    52021 acaccgccat ccgacgtatt gaagtataca ttggaagaaa atggctggat cgctatccgc
    52081 ccttcaggaa ctgagccgaa aatcaagttt tatattggtg taaaaggaag ctctacagaa
    52141 gacgcagccg aaaaagtagc ctcttatgaa gcgattatca aaaagatgac agaagaataa
    52201 aaaacttgag actgatggaa aaagtggtaa actttagaag tcagtctttt ttcaagttgg
    52261 aggtgaatgg attgccagac acgcaaaaat caaaagtaga gaaagtcagt caattattca
    52321 aaatgttgag tgatcccact cgtcttaaaa ttctgctata tctgaaagac ggggaacaaa
    52381 atgtgacagc aatcacccaa gcagtggaaa tggagcagtc tgctgtatcg catcaactac
    52441 gtttattaag agaaaatcat gtcgtcaagt ctcatcgcga gggaaaagcg attttgtata
    52501 gtcttgatga ttctcatgta ctggatattt tgaaccagac actcaaacat gtggaacatc
    52561 aataacgata ggtgaaaata gataaacgct ggagtgtgac aacgaagaaa tttcgttgtc
    52621 acactccagt gttttttaag aaaaagacaa aaatgattaa tcgctttttt ctgttgtttc
    52681 agttccataa taactatgat cttctaggtc taaagcattc aaaataagcg aagccgtttc
    52741 ttcaatagat aaagatgcca cattgatcac tacacatcct agtttttcat aaagatcatt
    52801 cgcaaaatcg agttcagctc taattttttc gatatcggaa taagcagtat cagggtttaa
    52861 tccgtaagag cgcatccgtt ctttacggat attatttaaa atatctgggt tattcgttag
    52921 tcccacgatt tttttaggat cgatttccca cagctgtttg ggaatatgtg cttggggaat
    52981 caaaggtaaa ttagcaactt ttaaattttt atttgctaaa aacaagctca aaggcgtttt
    53041 ggaagttcgg gatacgccta gcaaaacgac atctgcttct aaaaaaccac gaggatcttt
    53101 cccatcatcg tattttacag caaattccat tgctttgatc cgtttgaaat agttctcatt
    53161 caataaatgg agggcaccag gttgccgaat tggcttgata ccagttaaac gttcaatttc
    53221 tgatatcggt ggcgtcatca catcgaaact aaataattga tgttcttcac aaaaagaacg
    53281 tgttatctcg atcagttctt gattgattaa tgtatgcagg acaagtgctt tttcttctaa
    53341 tgcatcttgc aatgcctgcc ttaatgtttt ttcatctgtc acgaacgttc tgcggaatag
    53401 gttgaactca acggtaggat actgagccat agtagcttgc gctagttttg tcgcagtttc
    53461 tccagcagag tcggagatga caaaaaaagt aacgatttct gtagaatcgg aagacatgga
    53521 taaaacctca cttgcatttt ttttatgttt attataccat actctagaat gcgtacttcg
    53581 taatcatttt gatttggagg aataagtcat gaaaaaacag acattgatcg aacaatcatt
    53641 agaaacattg aaaaataacg gttttaaata tactaaaaaa agagaagcat tgttaactta
    53701 tttagtgaag cgtaaccgct acgtatctgc taaagaagtt tttgactata tgaatcagga
    53761 attcaaaggg gtaagttatg atacgattta ccggaatctc catgattttg cagagatcga
    53821 tttactggaa gaaaccgatt taaatggtga aatgaagttt cgttttcatt gctgtcaggg
    53881 ggaaatcggc catcaccatc attttatctg tacagtgtgc ggaacaacga aagaaatcca
    53941 tatgtgtcca atgaattatt tcgaagaaca gctaggtgga tgcacgatcg aagggcatag
    54001 attcgagatt ttcggaagat gtgaaaattg tcaataagga tttaaataaa ataacatttg
    54061 ataatttaaa aaaaaagcaa gatttccttg acggtcaaaa cagcattaga tataatattt
    54121 acaggacagt cttgtttttt gagatatatt tcaataacaa ttacggttac tttttaagct
    54181 cggagggagg gaattaacat gtcaaaaaca gtcgttcgca aaaacgaatc acttgacgat
    54241 gctcttcgtc gcttcaaacg ttccgtgtca aaagcgggta ctttacaaga atctcgtaaa
    54301 cgtgaattct acgaaaaacc aagtgtaaga cgcaagaaaa aatctgaagc agctagaaaa
    54361 cgcaaaaaat tctagttctg tgatgaattg gagttggtag tgtgtcacta ctaagtagat
    54421 taaacgatga catgaaagca gcgatgaaag cgaaagataa ggaatcttta caagtcatcc
    54481 gaatgatcaa gtcatctata caaaacgagc agattaaaga aggtcacgac ttgacagaag
    54541 aagaagaact aacggtgttg tctcgcgaaa tgaaacagcg acgtgattct ttgcatgagt
    54601 ttgaagaagc aggacgtgac gatttagctg aaaaagtaaa aagtgaaatt gtaatagtcg
    54661 aaaaatatat gccggaacaa ctttccgacg aagaaattcg acaacttgtt caagaagcaa
    54721 tcactcaaac tggtgcttca tcaatgaaag aatttggcaa agttatgggt gcaattatgc
    54781 ctaaagtcaa aggaaaagca gatggaaacc aagtgaatgc gattgtgaaa gaactattac
    54841 aatcttaatt ttatcattta aactgcggac agtgattgtc cgcagttttt tgtgttcgta
    54901 aaaaaagaac taaaaaacag ctagaagaaa gaataagatt ctttcttcta gctg
//
LOCUS       DACECY010000014        53149 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.015, whole genome shotgun sequence.
ACCESSION   DACECY010000014 DACECY010000000
VERSION     DACECY010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.015"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            156..797
                     /locus_tag="IWO38_000858"
     CDS             156..797
                     /locus_tag="IWO38_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0312965.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            828..1532
                     /locus_tag="IWO38_000859"
     CDS             828..1532
                     /locus_tag="IWO38_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ0312966.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMEQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQDFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            1529..2431
                     /locus_tag="IWO38_000860"
     CDS             1529..2431
                     /locus_tag="IWO38_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0312967.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQLG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     LDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2589..4295)
                     /locus_tag="IWO38_000861"
     CDS             complement(2589..4295)
                     /locus_tag="IWO38_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ0312968.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIAELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFHRLSQTELEPKAIQRQFQGIGFDTAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKNFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRDGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTEYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            4485..5108
                     /locus_tag="IWO38_000862"
     CDS             4485..5108
                     /locus_tag="IWO38_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312969.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIRQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            5558..6607
                     /locus_tag="IWO38_000863"
     CDS             5558..6607
                     /locus_tag="IWO38_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0312970.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDVLIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVA
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            6604..7485
                     /locus_tag="IWO38_000864"
     CDS             6604..7485
                     /locus_tag="IWO38_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0312971.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            7488..8258
                     /locus_tag="IWO38_000865"
     CDS             7488..8258
                     /locus_tag="IWO38_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0312972.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            8537..9391
                     /gene="aroE"
                     /locus_tag="IWO38_000866"
     CDS             8537..9391
                     /gene="aroE"
                     /locus_tag="IWO38_000866"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ0312973.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            9569..10732
                     /locus_tag="IWO38_000867"
     CDS             9569..10732
                     /locus_tag="IWO38_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ0312974.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            10849..11259
                     /locus_tag="IWO38_000868"
     CDS             10849..11259
                     /locus_tag="IWO38_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0312975.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            11433..13661
                     /gene="topB"
                     /locus_tag="IWO38_000869"
     CDS             11433..13661
                     /gene="topB"
                     /locus_tag="IWO38_000869"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0312976.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            13799..14116
                     /locus_tag="IWO38_000870"
     CDS             13799..14116
                     /locus_tag="IWO38_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0312977.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            14276..14512
                     /locus_tag="IWO38_000871"
     CDS             14276..14512
                     /locus_tag="IWO38_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0312978.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            14509..14907
                     /locus_tag="IWO38_000872"
     CDS             14509..14907
                     /locus_tag="IWO38_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ0312979.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(15574..16518)
                     /locus_tag="IWO38_000873"
     CDS             complement(15574..16518)
                     /locus_tag="IWO38_000873"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ0312980.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(16814..17758)
                     /locus_tag="IWO38_000874"
     CDS             complement(16814..17758)
                     /locus_tag="IWO38_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0312981.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            17985..19178
                     /locus_tag="IWO38_000875"
     CDS             17985..19178
                     /locus_tag="IWO38_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0312982.1"
                     /translation="MNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAVEL
                     LTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASIYGILPLFSQSYSIVLASRAG
                     LGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQYT
                     FLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIVVY
                     NAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSLLL
                     MGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPTNTSILLVGANLGT
                     FIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIRVDEEIKE
                     QLTTK"
     gene            19200..21215
                     /locus_tag="IWO38_000876"
     CDS             19200..21215
                     /locus_tag="IWO38_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HAQ0312983.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(21298..22671)
                     /locus_tag="IWO38_000877"
     CDS             complement(21298..22671)
                     /locus_tag="IWO38_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ0312984.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(22697..22981)
                     /locus_tag="IWO38_000878"
     CDS             complement(22697..22981)
                     /locus_tag="IWO38_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0312985.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(22983..24998)
                     /locus_tag="IWO38_000879"
     CDS             complement(22983..24998)
                     /locus_tag="IWO38_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0312986.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(25177..25482)
                     /locus_tag="IWO38_000880"
     CDS             complement(25177..25482)
                     /locus_tag="IWO38_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ0312987.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(25571..26602)
                     /locus_tag="IWO38_000881"
     CDS             complement(25571..26602)
                     /locus_tag="IWO38_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ0312988.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            26809..27684
                     /locus_tag="IWO38_000882"
     CDS             26809..27684
                     /locus_tag="IWO38_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0312989.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(27836..28678)
                     /locus_tag="IWO38_000883"
     CDS             complement(27836..28678)
                     /locus_tag="IWO38_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0312990.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(28770..30647)
                     /locus_tag="IWO38_000884"
     CDS             complement(28770..30647)
                     /locus_tag="IWO38_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0312991.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVCNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(30644..32065)
                     /locus_tag="IWO38_000885"
     CDS             complement(30644..32065)
                     /locus_tag="IWO38_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0312992.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(32068..35457)
                     /locus_tag="IWO38_000886"
     CDS             complement(32068..35457)
                     /locus_tag="IWO38_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ0312993.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            35714..37105
                     /locus_tag="IWO38_000887"
     CDS             35714..37105
                     /locus_tag="IWO38_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0312994.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(37178..37825)
                     /locus_tag="IWO38_000888"
     CDS             complement(37178..37825)
                     /locus_tag="IWO38_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0312995.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(37833..38519)
                     /locus_tag="IWO38_000889"
     CDS             complement(37833..38519)
                     /locus_tag="IWO38_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0312996.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(38525..39598)
                     /locus_tag="IWO38_000890"
     CDS             complement(38525..39598)
                     /locus_tag="IWO38_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ0312997.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            39820..40494
                     /locus_tag="IWO38_000891"
     CDS             39820..40494
                     /locus_tag="IWO38_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0312998.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            40499..41839
                     /locus_tag="IWO38_000892"
     CDS             40499..41839
                     /locus_tag="IWO38_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0312999.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(41988..42974)
                     /locus_tag="IWO38_000893"
     CDS             complement(41988..42974)
                     /locus_tag="IWO38_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ0313000.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            43698..44729
                     /locus_tag="IWO38_000894"
     CDS             43698..44729
                     /locus_tag="IWO38_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0313001.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            44838..46370
                     /locus_tag="IWO38_000895"
     CDS             44838..46370
                     /locus_tag="IWO38_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ0313002.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            46509..47321
                     /locus_tag="IWO38_000896"
     CDS             46509..47321
                     /locus_tag="IWO38_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ0313003.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            47311..47796
                     /locus_tag="IWO38_000897"
     CDS             47311..47796
                     /locus_tag="IWO38_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0313004.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            47819..48364
                     /locus_tag="IWO38_000898"
     CDS             47819..48364
                     /locus_tag="IWO38_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ0313005.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(48437..50080)
                     /locus_tag="IWO38_000899"
     CDS             complement(48437..50080)
                     /locus_tag="IWO38_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ0313006.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(50209..52437)
                     /locus_tag="IWO38_000900"
     CDS             complement(50209..52437)
                     /locus_tag="IWO38_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ0313007.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(52470..52949)
                     /locus_tag="IWO38_000901"
     CDS             complement(52470..52949)
                     /locus_tag="IWO38_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313008.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT        16188 a         9732 c        10108 g        17121 t
ORIGIN      
        1 aataatctct tctctttttt ctattatata tagattttct ctattttttg gtaagggagc
       61 tgtttcaaaa atggtaaaga gcggaaatgt tgcgaatatc agttatttaa tgatatgata
      121 ttcggaaaaa gaaatctcaa agggaggtaa cttttatggg attgaatgaa tttattttag
      181 gattgaacaa tttagagacg atcacaaggg cacctggatt ttttaaatat acagagcata
      241 cagttgcagc acattcgtat cgtgtagcat ctattgcgca agttctagga gacattgaag
      301 aagcgaatgg acaaccgatc gattggaaac tgctctatga aaaatcattg aatcatgact
      361 acacagagcg ttttattggt gatatcaaaa cgccggtgaa atacgcaagc aaagagttaa
      421 gagggatgct gcaaacagtt gaagaaaaga tgacggacga attcatcaac caagagattc
      481 cagctgagtt ccaagaagtt tatcgccgtc ggttgttcga aggaaaagat gaaaccgtag
      541 aaggagaact attagccatt tcagataagg ttgaccttct ttatgaatct tttgatgaaa
      601 tcatcaaaaa taacccggaa aaagtttata aggagatgtt tattgaggca gtgactacga
      661 ttaaaagctt tgaacatcgg ccttctgttc agtatttctt taaagagata tttccggaac
      721 tattgaacca ggaattttat ggaaaagagg cgtttttaga aaccgtctca acgattgtaa
      781 agaaagaaga aaaataaaaa tgatatgaga tgcaggggga agtatagttg aaaaagaata
      841 tggatgtcga atggggctat tctggagaca aaggtccgga aaattgggcg agtttatgtg
      901 attggtttgc gagaggtgcg gaatttccat tgcagtcgcc tattcatttg accaaggggg
      961 aagaaaccaa aatagaagga gacacgcttt cctttcatta tcaaaagcaa cggtttactg
     1021 acaaggaatt caaaaacacg attcatttag taccttttga ccaactcagt tatgtagagt
     1081 tcaagaaaga acgctattat ttgacagata ttcatttcca cttgccttct gaacatatca
     1141 tcaacggaaa acaggaagat attgaatttc attttgttca tatgaacagc aaaaaagaga
     1201 atctagtcgt gggtgtcatg taccagctaa tggaacagga aggttggctg tataatcaag
     1261 aaaatgggga aagttggaac ttagaaaaac atgagcattg ggtaaatccg gatgtgttct
     1321 ttccggagca aaaaagccat ttccattata ttggctcatt gacaactccg cctaccagcg
     1381 ggccaatcca atggtttgta atggatcatg taggaaaact gaaccaagat tttcttctcc
     1441 ccttcgatgg tcagtatgct cgaccgaaca accgaccaat ccagcccttg aatggaagag
     1501 aaatttatta tttcgaggag tttgagcaat gaatatccag cagatgaaat acgtggtcgc
     1561 tgtagcaaac aatggtagtt tcagagaagc ggcaaagaaa atgtttgtct cccagccaag
     1621 tctttcagct ggtatcaaag aattagaaac cgagttaggg atcacgctgt ttactcgaac
     1681 gaatcgaggg gcatatttaa cagaagaagg gcaagacttc ttaaatcgtg ccgagaaaat
     1741 attggtccag cttgatacat tagaaactca ctatctaacg agtgaaaaga gagacaattt
     1801 ttcgatcgct gcccagcatt atgatttctt aggtcctttg accgcacagc tactcgagga
     1861 attcaaagac gagatcaaac aatttcggat catggagacg acaacagcga gagtgatcga
     1921 agaagtcaaa gagcagcaca gtgagatcgg cattatttat atgaatgagc ataatcaagc
     1981 tggtatcaga agatatttac aactaggaga gctaagtggc cgcagcattg gaaaattcaa
     2041 aacgcatatt tttttaaggc aaggacatcc attagctgaa cacgcagaga tcaccaaaga
     2101 agaattgtta tcttatcctc aagttcgttt tacacaggat ggaaataatt tcccgtattt
     2161 ttatgaagac cttatcgaga tccttgatca agaaacagtg atttatacga gtgatcgagg
     2221 tactttgatg aatatcgttc ttgaaactga tgcttacgct tcaggatctg gtattgtgat
     2281 tggagggatt cgcgagcatt tgcggttaat ccctttggca gaaggcgaac taaatgaatt
     2341 ttatatcatt tattcggcga aacgtccgct cagtgaaatc gctcaaagat ttatcaaaga
     2401 gcttactcgg ttactggacc agcagagcta agaaaagcag atgagaacgc tgtttattga
     2461 acaccgttaa tcaaaaaaga ggggttgtga cagaattttg tcacaactcc tctttttgta
     2521 gcaaacaatt tataactaat tgctgttgtg ggaaagtacg caaatatgat tctagaaaca
     2581 ctcattgatt acctgttttg tttcatagct gtaatttttt cttcttctgg tgtcacgata
     2641 atcgtctttt ggttttcata aataacataa ccaggcttgg cgccatttgg ttttcggata
     2701 tgctttacct gtacaagatc aaccggcact tgggcagagt atcggtattt tgaaaaatag
     2761 gcagctaact cggcagcttc tgtaattgtt tcatcggagg gcttgtcact tttaatgatg
     2821 acgtgcgaac cgggtatatt ttttgcatga agccaatatt cggttttttt agctgtcttc
     2881 attgtcagct ggtcattttg aagattgttt ttaccaacaa gaatgagcgt tccgtcagaa
     2941 gaaaagtatt gatcaggctg agatggtttc ttacgttttt gtttttttaa gttcttcttt
     3001 ttcaggtatc cttctgaagt caattcttct ttgattgctt cgatatccat tgggccggca
     3061 atttccagtt gagataagac agattcaaga tattggattt catctttggc ttcttggatt
     3121 tgttcgccaa tcagcttaac agcgttcttc agcttttggt aacgatggaa atatttctgt
     3181 gcattttgat ttggtgtcaa tgccggatct agtgctattt tgattggccg atcttcttca
     3241 taataattag gtaaaacaac ttcttttgcg ccgcgtggta cttgtgtcag aaaagtagta
     3301 agtagttcgc catctctacg gtaattctca gcgttttcgg attctttgag tgtttgttca
     3361 cgttttttca atttattttt atttcgtttt agttcatttt cgatttttcg aatcagttct
     3421 ccgccttgtt gttttgcccg atctttctct gctttttcgt gataatagct atctaataat
     3481 tgactgagtg ttggataagc tgttacagca gatgcctgtt cggacaatac ctgataagca
     3541 atcggtgtga aaaaattttt attttccgtt tcgtaaaaag taggaatagg ctgatggctg
     3601 atggctgaga aaaattcgtc ccatacaacc atcttttcat taggacgttc cagtaatctt
     3661 ttggtcagct cttgagcggt atcgaaaccg attccctgaa actgacgttg gattgctttt
     3721 ggctctagtt cagtttggga aagccgatga aagatttttt ctttctcgga gctaaatggg
     3781 ttcaagacct cttgttttgg cggagcaaca tattctacac caggaagcaa agaacgataa
     3841 gtgttttgag agcttccaat gtgtttgatc gcatccagta tttttccagt ttctcgatta
     3901 actaagacga tcgtactatg acgtcccatt aattcagcga tcaatatgat attttgtaga
     3961 tcacccaact cgtttcgttt agcaaagtgg aaatggatca ctcgatcatt ttcgatttgt
     4021 tcgatgtctt ctaaaatagc gccatccaaa tattttcgaa gcatcatcac gaagttaggc
     4081 ggattatcag gattctgata atcgatttgg gtaatctgta cacgagcata acttgggtgc
     4141 gcagataata ataatcgatg gtttttgccg cgtgaacgaa tcaccagcac gatttcattt
     4201 tcataaggct gatggatttt cgagatccgg ccagatagta acgtttcacg caattcgttg
     4261 accatcgcgt gggtaaatac accatcaaag gacatggttc attcctcatt tcttttcata
     4321 agtatattca ttataacgaa aaaaagaaga aaaaccaatt tggccgaata gtaaagtaaa
     4381 agtctagaaa aatcttggac taattacata aaaaacagtg tttctatcgt tgaaatccaa
     4441 atttttaggt ataataaaaa ataggagaaa aagtagaaaa gaaggtgttt gttatttggg
     4501 aagtagttaa atcgagagta caagaaatga atgaacattt ctcagtgtca ttcgcagaaa
     4561 atccaagtaa gtattttcga cgtcttgtgt ttctgctcat cttatcattg ctgttaacag
     4621 ttgcaatgtt tttattgccg gaaaaaatcc gagaagagca agtaaatagt ctcgtttatt
     4681 cgtccatacc aaccagcaaa ttgaaacctt ttgattacag caaagctgat cagaaaatca
     4741 gacaaagccg ggcgatatcg gtgatgttca gcaaaccgaa cgggaaaacc tatcaggaca
     4801 tgcttgatgt ttttaatgat cctgagctta tggaagaatt gaatcgaccg atcttttatt
     4861 atccgatcgt ttatgatgta catgaactgg aacaaaaata taacatacga acggatgaag
     4921 tgacatttat tttttttgat ggaggaaaag aagcaaatcg gataactgcg ggtaagggag
     4981 gaataactga ttttgataag gagttgatcc ctgaactgaa tcgacttccg ttagccaata
     5041 tcaaacaatt agaagaagaa ttggatagaa cgggagtaac ttcccaaaat tccgagattg
     5101 ctaattgaga cattggcgcg atgaaacggt gggtctatat agcgctattt ttaattatca
     5161 gaacaagtga tgtttgatct tcaaaataac aaataaaaaa ttaaaaaata tagttgacga
     5221 aattcaaaaa ttcgattaga cttaaatcaa ttcttaagga aagggtgacc tgtaatgaac
     5281 ttatcaaaac gtatttcagc agaaaacaaa aaaaattggc gcagctggcg tagataagtt
     5341 gtattaatgg tgagactttc ccatagatac aactttttag agtgcatttc taaagcttgt
     5401 atctatgcta gacatgaaca ttgactgccg catagattag aatctatgcg gttttttgtg
     5461 taaagacaat gaccgctttg gccattgtct ttttttgtag actggatact tgcctaatta
     5521 taataaataa aaagattgac tttgcaggag gaaaaaaatg aaaaacaaac gattaataac
     5581 agttgtagta attattttag tttatttagc aggcgcattt atttatggaa aagcctcagg
     5641 cgaaaaagaa agtacttcga cgaaccagac aaaaaaaaca acagttgttg gggtattgca
     5701 atacgtgagc catccggcat tagatgagat ttatcgagga atcaaagatg gtctgaaaga
     5761 agaaggatta aaagaaggag aaaatcttga gatcaaattt caaaatggac aagccgatca
     5821 gagcaagcta gccacaatga gtcaacagct agtacaatct gatccagatg tcttgattgg
     5881 aatcgcaaca cctgccgctc aatcattagc aaacacgact tcatcgatcc cagttgtgtt
     5941 aggtgctgta acggatcctg tgggggctgg attagttgct tctttagaga aacctggtgg
     6001 taatgtaaca ggtgtttctg atcagccgcc tgttgcttca caaatcaagc ttgcttctga
     6061 tttgttgcca aatgcaaaaa aagtcggaat cttgtatgct tcttcagagg ataattcaaa
     6121 ataccaagta gcacaagcag aagaagctgc aaaaaaagaa gggttagaat cagtaaaata
     6181 tgcggtgcct tccactaatg agattgcaca aacggttcaa gtgatgagcc gacaagtcga
     6241 ttttatctat gttccattag ataataccat tgccaatgcg atgcaaacag tggtaaaaga
     6301 agcaaacaaa gcaaatatcc ccgtcattcc atctgtggat acaatggtgg aacaaggtgg
     6361 actagcaaca atcgggatca atcaatatca attagggctt caaagcggta agatggcagc
     6421 agaacttgcg agtggaaaag aaaaaccgga aacgacgcct gtttatacat ttgatcaagg
     6481 ggatacagtg atcaatcaat ctcaagctga tcgcttagga atcattgttc cccaatcgat
     6541 gaaggaaaaa gccaaaatca ttacagatga ctcgcagcaa gaaacatcta aggaggatga
     6601 caaatgatcg tatcagctat ttcacaaggc atgctttggg caatattagg attagggatt
     6661 ttcatgactt atcggatctt agactttcct gatatgacga cagaagggtc cttcccatta
     6721 ggtgctgctg tctgtgtgac agctattact catggggtag cgccgattgt agctacatta
     6781 ttaggagtag gtgctggcat gatagcagga ctcgttacgg ggttacttta tacaaaagga
     6841 aagataccgg ttattttagc agggatttta gtgatgtcag gattgaactc agttattttg
     6901 tttgtgatgc gaacaccaaa tctttcttta ttgaatcatc cgattctaca agacagcttc
     6961 catcgctttg ggttacctga ttactttgac acagtccttc ttggcttcat ctctttgggc
     7021 atcttgattg ctgtactgct attcttcttc aatacagatt tgggtcaagg ctatattgcg
     7081 acaggagaca acgaaacgat ggctcggtcg ttagggataa aaaccgatcg tatgaaaatt
     7141 ttaggtttaa ctttgtcgaa cggtgtcatt gcattatccg gtgcgctgat tgcccagaac
     7201 gacggatatg cagatgtaaa taaagggatt ggcgtaatcg tcatcggtct tgcttccatc
     7261 atcatcggtg aagtgatctt ccaagaattg acgctagcag aaagattgat tgctattgtt
     7321 gtcggcagta tcatttatca gctattgatt ttagtagtaa tcaaactagg gtttgacacg
     7381 acttatctga agttgttctc cgctatcatc ttagctattt gtctgatgat cccgcaattg
     7441 aagaaagcac tgaacttaaa aacgggtttt gagaaggagg catagtaatg actgatatat
     7501 tgacaatcaa aaatggcaga aaagtcgtaa acaatggaat gaatgaagag aaagtactat
     7561 tggatgaaat taaccttaca atcagagaag gggacttcat cactgtcctt ggcgggaatg
     7621 gtgctggaaa aagcacactg ttcaatacga tcgctggtac gcttcaactc agtcaaggat
     7681 cgatctcttt taaagatcag ctgatcacaa aagattcaga agaaaaaaga gcacgattcc
     7741 tttctcgagt tttccaagac cccaagatgg gaacggctcc tcggatgaca gtagcagaaa
     7801 atttgctact tgcacaaaaa agaggtcaaa aacggaaatt ccgtttacgt aacttgaaaa
     7861 atcaaaaaga agattttttc agactatgtc aagaagtcgg taatggccta gagcaacact
     7921 tggatactcc agcgggtgaa ttatctggtg gtcaacgcca agcattaagc ttgctgatgg
     7981 cgacgatcac agctcctgag ttactattat tagatgaaca tactgcagct ttagatccta
     8041 aaacagctaa agcattaatg cgattaacaa atcagcggat caatgaacaa aaattgactt
     8101 gtctcatgat cacgcatcga atggaagacg ccttgaaata cggaaatcgt ctgatcgtac
     8161 tccaaaaagg gaaaatcgtt caagatattt ctgaaaaaga aaagcaacat ttgacaatgg
     8221 gcgacctttt gcagttcttt gaagaagtag tggattgatt tcgacagaaa aattcctttc
     8281 agtagaagga cttgaaaact cattgacatc ttcctttgat tcatcctata cttaaaacaa
     8341 taaacaatta aacatatttg ggtgttttgt aggatcacgt agtcaatgat tttaacaatt
     8401 aaatagaata ttctttgctc tgacaagtaa acgatgagaa aagaatttgc tactttgcca
     8461 cctagacgtt tcgtactttt tcgtatcaaa cgctaagtgg ctttttttat tgaagtaaga
     8521 acaaaggagg aataagatga tttctggaca aaccaaatta gctgggctct ttgctgatcc
     8581 ggcttctcac agtttatccc cattgatgca caatactgct tttgaagcaa acaacatcga
     8641 tgcggtgtat ttagctttcc aagtaaatcc aatgaatttg aagcaagcag tcgaaagtat
     8701 tcgaactttt gatatgttag gtgtaaatct ttcgatgccg aataaaacag cggttattcc
     8761 ttatctggat gaggtaagcc aggaagcgca attgatcgga gcagtgaata cgatcgtgca
     8821 cgaaaagggt cgtttgattg gctataatac cgatggtgcg ggcttcatgc aatccgtgca
     8881 agaagctggg atttcgatca aaaagaaaaa aatgactcta ctgggagctg gaggtgcagc
     8941 aaaggcagtc actgttcaag ctgcgttaga cggcgctgag gaaatcgtag tttataaacg
     9001 aaacaaccaa acattcgctc aagtaaaaga aacattcgaa caactcagct atcgaacttc
     9061 ttgtaagata aaagttcgtg actttgccga tacgcatcaa ttaagaaaag acttgaaaga
     9121 aagctgtctt ctagtcaatg caacagatat cgggatggga aagaaaatca atcagtcacc
     9181 gctcttagat ataagcatga tgcatgagaa tctagcggtt tttgatttga tttatgcacc
     9241 tagagaaaca cgtttattaa aagaagcaaa aaaagtagga gcaaaaggat acaatggact
     9301 tggcatgttg atctaccaag gagcaatcgc gtttgaatta tggacaaagg aaaagatgcc
     9361 gatccatgtg attgaaaagc tgtttgatta aaaaaggata atagataaag atcttttatt
     9421 ctcgcataaa cggtctcaag aaaagtaatc ctttatgtat cgaagcattt atttcgttat
     9481 tttttattaa attatcaaaa aatgataggc ttttgtcgat tggttatcgt atcctttatt
     9541 tatcagtaag aaacgaggag gaaccacaat gagtcggatg gatcgctata aggacatcca
     9601 taagaaggcg aaacctctga agcgggatac ggggagtatt tttgctagaa gagaacgaaa
     9661 acgaacgcgt atggaaaatg aggaagatgc tacccgtatt tatgaaagag agcagcattc
     9721 atttgaacat ttgaaccaat acgagcaaca gatatacaga gatgcaccaa gagcagaaga
     9781 acaacaaaaa aataaaaaat ttttcaatcg gcaaaagaag aacaaaccgt taaaacctcg
     9841 aagaaagcga tcgtggaaaa aaatcattct atcgattttg cttgttttga tcctttattc
     9901 aggtatctca ttttttattg gtaaatatgt agctgaacat gacacaagct tagaggcagc
     9961 gacttcagaa acctttcatg ggcaaaaagc gacgaatggt gcgcataata ttttgatcct
    10021 tggaagtgat acacgcggag aagatgccgg gcgagccgat acgatcatgg tcttacagtt
    10081 ggatggtcct tcccataagc cgaaactgat ttcttttatg cgtgacagct tcgttacgat
    10141 cccaggggta ggacaaaata agatcaattc agcttatgca tacggaggag ccgatctagt
    10201 gcgtcaaact ttagtagaaa attttggaat cgattgtcaa tactatgcga aagtcgattt
    10261 caaatcgttt gaaaaagtga tcgatgcctt attcatgaac ggagtgaaga tcgatgcaga
    10321 aaaagatctg aatctggatg gtgtagatat taaaaaaggg gtccagaaga tggatggaca
    10381 tgttcttttg cagtacgcac gtttccgaat ggatgaacaa ggagactttg gccgtgtcag
    10441 acgccaacag caagtaatga acgctatttt cagccaattg aagaatccat tgaatctgat
    10501 ccgtgcacct tatgcagctg gtaaagcttt aggatataca tccacagatg tcactgcttg
    10561 gttcttgacg aaaaacctct tttcgattgc acgaggagct ggtggcattg atcgaataag
    10621 tgtgccagta gacggttcgt ggaattatgg gcaaagtgat tatgctggca gtattcttga
    10681 aattgataat caaatgaatc aatctgctat tacagatttt ttgaataaat gatcataaaa
    10741 aagatcaaga agaagctgcg caaggctttt gtcatgaatc aatcaaacga ttcactcgta
    10801 aaaaacgctt tcttatggta gatttaacca agagaggagc tgtttacatt ggatatctat
    10861 ctgaacttca aaaatgagtc gcgtgaagcc atcgcttttt atgaaaaagt ttttcagact
    10921 gaagcatcag gtatcaccac atacggtgaa atgcctggaa tagaacaaat gaacttgcca
    10981 gaagaagcaa aaaatctaat cacgaatgca cggctgaaca tttatgatac attagttatg
    11041 tttgcagata cgccagaagg aattggtcct gaattagttg tgggaaataa tgtttcttta
    11101 gtcatccaga caagaaatga agaagaaacg aagcgcctat ttgaaagctt agctgagggt
    11161 ggtaagattg agatgccact ggatgctgtt ccgtgggctc agttgtatgg gatggtaact
    11221 gatcgatttg gcatcaactg gcagattaac caagagtgac agctaaagat gaatattttc
    11281 tggaaacatc aagcagcaaa cggatcatcg tttgtttgct tgatgttttt gtgtctattt
    11341 cgactatacg gtatcttttt tttattgcca gatgcgaaaa tatgttccat aatagagaag
    11401 tccgcaaaaa attaagtaac ggaggtcaga agatgaagac ggtgatacta gctgaaaaac
    11461 cttcacaagc aaaagcctat gccgaaacct tccaacagca aaagcggcat gaagggtatt
    11521 atgaaatcag cgatccattg tttgctggtg aagtaaccat cacttatggt tttggacatt
    11581 tggtagatat ggttgctcca ggagcttatg aagagcgttg ggcaaaatgg tctttagaaa
    11641 atttgcctat tttcccagaa accttccgtt acgaagttcc aatggacaaa cgtgcacaat
    11701 tttcgatcgt aaaacgagaa ttgcagtcag ccgataccat cattattgca acagatggtg
    11761 atcgagaagg tgaagccatc gcgtggtcga ttattcgaca agcaaacgca ttttctaagg
    11821 aaaagaacta tttacgttta tggatcaatt ctcttgaaaa agaagcaatc tatgaaggtt
    11881 ttaagaatct ccaacctgga aagaactatt ttccaaaata taaagaagcg caagcaaggc
    11941 agaacgctga ttggctgatt ggaatgaatg gaagtccatt atatagcttg ctacttcaac
    12001 gaaaagggat tgatggcagt ttttctcttg gtcgtgtaca aacaccgaca ctctatatga
    12061 tttatcagct gcaagaagaa atcaaacact tcaaaaaaga aacgtacttt gaaggagaag
    12121 gtttaattac gacaaacaaa gggcagtttt ctggaaaaat cgtaccaaaa aaagcattta
    12181 aaacccaaga agagttaacg aaggaaatag aaaaacttgg agcgcattta ggtaagcaaa
    12241 acggacggat tcttgaagtt acgaaaaagg aaaagaggtt gcatagtcca cgtttatttt
    12301 cgctatctag tttgcagaca aagatgaatc agctaatgaa agcaagtgcc aaagctacac
    12361 ttgatgctgc tcaaggattg tacgaaagca agtttctaag ctatccaaga acggattcct
    12421 tttacattac agaaaatgag catcagtatc tggttaaaca tctgcaaaat tataaaaaat
    12481 ttttaggaat agaagaagta cagacaactg agatcaaagc aaagaaacga tatgtcgatg
    12541 cgaaaaaagt tcaagaacat catgcaatta ttccgacaaa gacgattccg actcctgcac
    12601 gctttgcgaa gctttccaaa ttacagcaag caatctattt gcaagttttg cgcactactg
    12661 tggctatgtt tgctgcagat tatatctacg aagagacgac cgtaatgacg ggcgtacagc
    12721 aattaaagct aaaaagcatc ggaaagatcc cattacagca aggttggcag gaaatcttac
    12781 gcatcaagtc aaaaaagaaa gaagctcaga ctcttcctcc tatcgtagaa aatgaagaag
    12841 cagaagtaga actgaaaagt atggagaaag aaacacaacc tccaaaaccc tataccgaag
    12901 gaacgttgat cacagcaatg aaaactgcgg gaaaaacgct ggataatgat gaagcgcaag
    12961 aaattctcaa agaagtagaa ggaataggga cagaagctac acgagccaat atcattgaaa
    13021 atcttaagca gaagcattac atcgaagtca ataaaaacga aataactgtt acgccaaaag
    13081 gaatcactct ttgtaaggca gtagccaatg aaccattact gacgagcgca gaaatgactg
    13141 cgcgctggga agggtacttg agaaaaattg gtaaacaaga ggggacacca tctgtttttt
    13201 tagagaatat caaaaaattc atcctacatt tattggcaga agttcctaat caaatcgaca
    13261 aggtagactt ttcagatgag attagcggga cacgtcagat aaaagcgatg gagaagaaaa
    13321 acgatcaatt aggaagatgt ccaaaatgta aaaagggaat agtcatgctt tatcctaaaa
    13381 tcgctacatg cttgaatccg gaatgcgact tcaaattgtg gccaacagta gctaaaaaga
    13441 agctgactaa aacgaatctc agagatttgc tttcaaaagg aaagacaagt aaagttgtca
    13501 aaggttttac tggtaaaaaa gggaaattcg atgcagtttt agtattaaaa gaagatatga
    13561 caatcggttt ttcttttcca tggatcgaaa acagtggaac aaaagcagtt gatggaaaca
    13621 aagaaacatc aagaaaaaat aatgaaaacg aagtagagta aatgaaaaca atgaaatagc
    13681 ggaatattta ttccgaaaag tgagaatata aataccaata aaactaaaaa atatcagtga
    13741 atcaagcaaa aacgttgggg taggctgatt ttatatagat agcaaggagt ttttttattt
    13801 gaaaaaagtg cattcatttt taaattatct tatgggagca atcacgattg tggctgtgta
    13861 ccaagcattt ctaaagtatc ctgattacgg gacgaagttg ttgtcattga cgcacaatca
    13921 aacagcatat tcaccaacgt tgattttatc gactttgact gcaggagcta aaagcgctac
    13981 ttattttttt cataaaacga caagcatgct aaagcgaaag aaacagcgaa aacaattgtt
    14041 ggtggtggta gcggctatga tcattattta ttttctacca gttattacac aagtatttca
    14101 tcaatatttt ggttaagaat acgagagttt gtgacaaaag tcatgtcaca aacttttttc
    14161 tttgtcattt tttcgtaaga aaaagcaaaa taaaagtata tacattgatt aaaagtatat
    14221 acttggatat aatgtactta ctatagaatg agaatggtat agagggtggc gaaatatgaa
    14281 aaaaatgaaa aagtataaaa ttagaaaatc tggcaatagc gatgtaacaa ctatcccacc
    14341 agaagtaaaa gaattcatgg gtgtgcagac aggtgacgca atcagttatg tttttcaatc
    14401 tgatggttct gtacgaatga tcaaagcaca agaagaacct gatattgatt cattagttga
    14461 ttcgatcatg aatcaatatg aagacgcgtt aaaagatttg gttgatttat gaggtatctt
    14521 tcgactaaag aaattatcaa aatcaacgcg aaagttattt tacgtcactc gccaggcgaa
    14581 atgatcggtg taaaagatgc aaatgcatta gatatggccg tcaaacagcc ttcacaagca
    14641 gtattcaatc aagaactcta tcctgaaatc tatgaaaaag cggcgatttt agcaattaat
    14701 ttagctaaaa aacatccttt tcataatggc aataaacgaa ctgctcttgt tgccatgttg
    14761 ttgtttcttc aattgaataa ttgtccagtt gctttttctc gtcaagaagc tgttgatttt
    14821 atcataatga ttaccactag ttcattggat ttcgattcac taaagtccaa gataaccagt
    14881 tatttacgac aaaccgcaat aaaatgaacg aattaaaaac tttatttctc aattagatca
    14941 gcatgtcatt aaggaatatg ctgatttttt tatgtttaat catacaaaat agttttttca
    15001 taaaaatatg gaaaatatgg attaattttg aaataattga tttaaaagga atacaaaaga
    15061 gtgaatagta ggaaatagtg gtttagaact agtgggctga ttttttatat attttagttt
    15121 acataatata tattatacaa agtagattta aaaaatgata aacctctctc gaacggtttt
    15181 ttatcaaaaa tcgtcatttt tccatcaaaa tctgcttatg caaaattata aaaaaatttt
    15241 gcagtttttc ttactttttt tgtcgtttaa ttgaatgata aaaaccagtt tctttattca
    15301 gttaatcaaa tatgaacgaa ttagatatga atcattgtat ttgccgttgc ggaaggattt
    15361 tttaattaac cacagataaa cattgataac gattcgctta ttgtaatcaa cagattaaat
    15421 ttcatatgtt catatgtacg gatacatttt cggaccgatt aattttaacc attctgttta
    15481 tttgcttacc tattagatat caataaaaaa ctgcttaaca gctatttcag ctgttaagca
    15541 gttttaggat ttttagttca tattcattgt ttcttataat acagtgttca atacttctct
    15601 taaagcactt gttgatttgc tgaacattgc tttttcttct tcgttgattg ataattcaac
    15661 aacttcacga acaccatttt cgtcaacgat tgatggcaca cctgtaaaga tatctttttc
    15721 gtcgtattca ccagttaaat aagcagatac aggcaataca gcttgttcgt tattcaagat
    15781 agcttttacg atacgtgcag tactcatacc aataccatag taagttgctt ttttacgatc
    15841 aatgatctca taagcagcat tacggacttt atcagcaatc acatctaatt catcttgggc
    15901 aatacgatgg tctttttcta caatttcaaa cactggttta ccaccgacag ttgtatgtga
    15961 ccatgcagca acttctgaat caccatgttc acctaagata tagccgtgga cgctgcgtgg
    16021 gtcaactttt aatttcaatg cgatttcttt acggaaacga gttgtatcca atgttgttcc
    16081 agtaccgata acacgtgatg ttggcagacc agattcgttc caagcgatat atgtcaaaat
    16141 atctactggg tttgaagcaa caacgataat accgtcgaat cctgatccca tgatttcttt
    16201 tacgatttgg cgcatgattg acgcatttgt tttaactaaa tctaaacgag attgaccagg
    16261 tttttggttg ataccagctg tgataacaac gatatcagca tctttacatt cttcatagcc
    16321 acctgaccat acagccacat tttcatcgcc ccaagccata ccatctaaaa ggtccaatgc
    16381 ttccccttct gctttttctt gattcacatc gatcaatact aattcattag agattccttg
    16441 gttgatcatt gcataagcga tacttgttcc aacaaaacct gtaccgacga ttactacttt
    16501 acgacttgtt tttttcatga ataaacagct ccttttcata actatttgtt actttcatga
    16561 aaagtatagt cttgtttatt cattttatca acatgttaat tataatagtt gttattataa
    16621 tttttgatgg aaacgaattc ctgaatacgc ataacagata aatgacaaat gtttacacct
    16681 atatcccctg ataatacaac caaaagttta gaactgaaaa taaatccaaa aaaattcgct
    16741 gcaacgatat aataagacag caatcgcacc taaaacaatt ttccttcata tgctttagtg
    16801 aaagattttc ttcttaatgt ttatttctcc attcattggg agtacatgtt gaatacttct
    16861 tgaataattt gaataaagaa gtcgtttgtt tatatccgat acgattagcg atttcttcca
    16921 acgaaaaact cgtttcttga atcagttcca acgcaaagtc gaattttttc tttagaacca
    16981 gttcttgaaa ggaagcatca aaatacattt tcaatttatt actaagatag accggattgt
    17041 agccaaaatg agctgctgtt tcttttaacg tacaagaagt aaaatgctta tcgatataat
    17101 ctaatactct aagcatctca ttttctgcgc atccatctgc tgatgaaaag ctttgttgtt
    17161 ctaataaatg agtcaattcg atcagtaata gtgatgtata gagatttaac gaacggatct
    17221 tatgactgta tgtatcaaaa taaatcaaca gcatattttc aatcaaatgc gtcaaacgac
    17281 ttgtttttcc gccgcgaaaa tgaatgaatt gtgtatgttc ccctttttct tttgatgcat
    17341 ttatcaaaaa agaagaaagt agattatttg tgcttgccaa atgattcaat atctctgtag
    17401 ataaggtttc ttcttttaat aagatgttga ttaaaatatc ctcttgccct gtgtattcga
    17461 ttctttgagt agcttcttta tcaatgatca ccacgtcact ttcttccatc tctatccttt
    17521 ctccattaat aatttgtaaa catgtaccgc tatacatata attgatttca acaaaatcat
    17581 gtgtatgctc gggaacttcc gcatatcgac ggtgtttact gatatgaata tttccctgtt
    17641 gaaatagatc ccctcggtgg atttttggcg gtgtctgaag atggtaagct aagtttaaat
    17701 cattgatatt cattccttgt tcacgctgca gagtttcgat tgattcttcg ttacgcaaaa
    17761 aaaatgtcta attcagctag ttccaattga ctccctcctt tttgtaagcg gtttctttcg
    17821 cttattagtt cacgctgtac tgctattata tgcaaagcta tttttggttt cataaagact
    17881 ttctatggtt gggacatgaa cttcctgtga ttccgctatt gagagaataa ccacttcata
    17941 ctatattttt gataagcgct tacaaaaatg aaaggaacat gcttatgaat aaaaagatga
    18001 tgaaattttc tctgctgtca gtctctctta ttttgacttc tgctggtgca gtcacgtctg
    18061 caattcccag tatgtcagct acttatgcca cgcgttcttt atctgctgtt gaattattaa
    18121 cgactgctcc ctcttttatg gtcactattt ttgttttgat tagtagtttt attgctagga
    18181 aaatcggaca aaaacaaact gtgatcatcg gattagtgat tgcatcgatt tatggtatcc
    18241 tccccctctt ctcacaaagt tacagtattg tcttagcttc aagagctgga ctaggtattg
    18301 gctttggatt gatcaattct ttggcagtca gcatgattgc tgccttttat cacggagatg
    18361 aacgagcctc tatgattggt tttcaaagtg catttcaagg atttggtgca gctttaatga
    18421 catttgttgc aggacagcta gtacagttcg gttggcaata taccttttta gtctacgcca
    18481 tcagcttacc tattcttgtc ttgtttgttt tgtttgttcc tacacctgaa acaactgaac
    18541 ttcaatcaca tggcacagcc ccaacaagac aagtaatcaa aacgaatcct aaaatgatta
    18601 gttatggtat ttttctcttg ctggtcatcg tggtatataa tgctgtatcg attaaattag
    18661 ccacagtcct gacgacttat tctctaggaa acgaaactaa tgcagcgaat atcttaacaa
    18721 tcatgcaatt ggcaagtatg gctgccggag tttgttttgg ctggcttcag acaaaagcac
    18781 atcgctatat gcttacactt tctttactac tgatgggaat cggctttgta ttgattgcca
    18841 ctgtgataaa cctgtatgtg attgggtttg gtgcgatcct aacaggaatt tctttttcgt
    18901 tattcattcc ttacttattc aaccaagtaa gtatccaagc gccaaccaat acttcaatct
    18961 tattggttgg cgctaattta ggtactttca ttgcaccata tggtttgctc tttctctctt
    19021 ttgcttctct tgggagtaaa acacttgctg ttttcgtcaa tggcagtatc ttgctgttga
    19081 ttagcgcgct tattagcctt ctagtagtac acaggacgaa tagaaagaca gtgataagag
    19141 tagatgaaga aataaaagaa caattgacaa caaaataaat ttgatggagg tcgtgtaaaa
    19201 tgacaaatga aaaacataaa gatagatggc tttggtatcc tggagacttt gagatccgcc
    19261 atggactgct acaaaatttc caacgggaag aacgaggttt tgactggcca gcgtactggt
    19321 acatggatga ttgccatcgg aacgtcaagt ttaagagata ttactttctt gatcaacctt
    19381 ccatgtttaa agtgacgatc caaggagttg ggtatgtaga aattaatgga caaaaacatc
    19441 catgtggaaa atggctgact tgtccagctg gtaaagcaaa aattcgtatc tttgtaggac
    19501 atacatcagg gctaccagcg atgtttatcg agggagacga agtaaaatca gatattggct
    19561 ggacagcaag taattttatt gaagaatatc cagctggctg gagtccgctt tatgtcgata
    19621 tcgcaaaaga tccaaatcaa atctattatc aaaaagaatg cgtccatcca gtcactgaaa
    19681 cggagattgg cggaggaatc ttattcgatt ttgggcgagc agtgaatggc tgtgtagaac
    19741 taatatctct ggatgaccat atcgaatcta tcactctatg ttatggagag tccgatgtag
    19801 aagctatgga tatcgattat tgctactata agcaaatagg aatgacaaga aaaatgaaag
    19861 ctcgtaaacg cgcttttcgc tatttgttta taccagaggt ttctactgga atggttcaac
    19921 ttcaagtttt tcatgaattt ttatctcttt cgaaacaagc tgctttttca agcgattctt
    19981 ctttactgaa tcagatctgg tcagtatcag aagaaacctt caagttatgt agtgatttgt
    20041 tttttatcga cggtatcaaa cgagatcgct ggatttggtc tggagatgcg tatcaatcat
    20101 tcatgatcaa tcaatatctt ttttttgacg aggaaatcaa taaacgaacg atattagcat
    20161 taagaggaca aaatgaaatc aagcagcata tgaatactat cgttgactat tcgatcttgt
    20221 ggttgattgg tatcgaaaac cactatatga tgacgaaaga ctatgaattt ttacgcatcg
    20281 tctaccctaa gatgcaatca ttaatgagat acttgatgga gcaaacgaat gaattagggt
    20341 ttatttatgg gagagcaaaa gattggatat ttattgattg gtctgaaatg gataaagaag
    20401 gaacgcttgc tgcagaacaa gtcttattac tgaaagccta tcattccatg atcacttgcg
    20461 gagatatctt agctgaaaca tatggagaga acgaaaatta tcatactcat ctctatcaag
    20521 aaaaatatca actattgaaa caaaacctat ttgactactt ttgggatgaa gaacttggcg
    20581 cttttatcga ttgctatgaa tctgggagaa gaaatgtgac gcgtcatgcg aatattttcg
    20641 ctgttttatt cgattttgct aacaaagacc aacagcaatc gatcttaaag aatgtcttat
    20701 tgaatgacac tatcacacag attactactc catatttcaa attctatgaa caagatgctt
    20761 tatgcaaact cggacagacg aatatcgtct accaaacgat tcttgattat tggggaggga
    20821 tgttatctca tgatgcggta actttctggg aagaatacga ccctactcaa acaggaaatg
    20881 aaaagtacag tatgtatgga gatccttatg gaaaaagctt atgccatgca tggggagcaa
    20941 gcccaatcta tcttttagga cgctatttta tcggtttgta tccaacaaag ccaggttatg
    21001 aagaatttat gatacaacct aatctagatt catttgaaac actgaattgt acgctaccta
    21061 tcaaagatgg aaagatttcg cttgaaaaaa caaaagataa gatcgtgatt acttctgata
    21121 gaacaggcgg aacagtgaaa atgaatggta aaagttatcc tcttctagca aaacagccac
    21181 ttgtcttaac aagctattca aaaataaatg cataaaacaa aaaactgctt gcgacatatc
    21241 agaagacttt aaatcttctt ctgatattgt ccaagcaatt tttattttga agtttgctta
    21301 attctgttct ttagaaacaa tgtttgttct ttttccggca aaaagaaagc tagataatac
    21361 gacaagtaaa aagaaagcga tgattccaac tgtcaaggct atttgaccgt attctcctag
    21421 tttcccaagt acaagtcctg gtactagata atcagtatcg gcaaatgtag aagaagcgat
    21481 tttaagatct cctaatactg gaaggataaa taatggcagc caactaatca ataagctatt
    21541 gaaagcagaa cctaataccg cccctcttct accgccagtc gagttgccga agacaccaga
    21601 agcagctccg cagaagaaat gtcctactac cccaggaatg ataactgtag tttgcaagcc
    21661 tatcatagca aacatggata gtgttcctac aaagaagcta acaaaaaagc caattaatac
    21721 agcattaggt gcatatggaa aaacgatagg aacatctaat gctggtttag aatcgggtac
    21781 gagtttttta gcaatccctt ggaatgctgg aacgatttct gccaacacca ttcgaacccc
    21841 ttgaaggacg ataacaaacc cagctgtgaa tgttccagct tggatcagtg caaagattac
    21901 agcgttgtct ccattgctta gttctctttc aacaaatcca ggaccagcta acaaagcaag
    21961 tgcgacatat acgatcgaca tcaagagcat gatcgataca gtagaatctc gcaaaaagct
    22021 gaaactttta ggaatcttta tgtcctctgt agaacggtct ttgtttccaa aaaactttcc
    22081 gatccatcca ctagaagcat aaccgatatt tccggtatgc cctaacgcaa cttgattgtt
    22141 tccagtgatg ttcttcataa acggttgaca aagagcaggc gtaatagtta atagtgttcc
    22201 ttcgaagacc ccgcctaata agatcatcca caaatttgca gtaaaccccg cgcttactaa
    22261 aatgactgct gttagacaag aaacataaag cattgcttga cctgtaagaa aaatgaattt
    22321 atatcttgtg aatctagcaa gtaaaatatt cacgatcatc cctactagca tgatcaaagc
    22381 agtaggtgta ccgtattgtt gcaaagcaat cgccactact gcttcattgc taggaactac
    22441 tccttgagta tgcagagctg tttggaacat cgaagcaaaa ggggttagtg aattactgat
    22501 taaaccagca ccgccgatca gtactaggaa tccgacaaat gttttgatag ttccttggat
    22561 cacttctgaa aatttcttct tctgaagaac gagaccaagc agtgcaatca aagaaacgag
    22621 gatggagggc tgactgacga ttgacacaaa caaattcaac aatttatcca ttgatagttc
    22681 ctcccttgaa tcatttttat tcttttgaca agatcttcgt tactttttct tttacttctt
    22741 cgctgtcaat gatcgattgc aaaggtacga gcttttcatc aggtatagaa gttagcgtat
    22801 ctttcaaaga atgctcaatg aagaaataat cagccatatc gctagataca cttccaacat
    22861 ccgtatgact tacttcaaac tcgtccacat ctgctccaag atctgcaagt gcttgtttga
    22921 tgttcatctc taccataaaa cttgttccta atccactttg acatactgca aaaattttca
    22981 tatcattcgt ctccttttct tatgatcgct aatatttctt cttttgttga tgcgtgtaat
    23041 aaatcttgaa gattttgttt attagaaagt agctttgtta aatttgccaa agcccgaaga
    23101 tgtgcctcat tatcgatagc agctaaacag atgaacaaac taatgggatg ggctggatta
    23161 tcaagcagat ccacaggttg ttgaagtttc agcaaagaca tccctacttc gtttacgcca
    23221 tcttctggac gagcatgagg taatgcgatg tgatcgccaa tatggataaa cgcaccatac
    23281 tgctccacgc gctcgatcat agctttgata taactttctt ctattttatt ttctgataat
    23341 agcggctgtg cggcaaatgt gattgcttct tgccagtttt ttggttgatc cgataattgg
    23401 atcatttctt ctgttaataa ttcagatagc ataggacgtt gatcctcctt cttttctaaa
    23461 agtttgttca ttttacgatt taatacttga tagagctttt cttcgtctac gccttctttt
    23521 aattcgatat aaggcagtag cgccgacaac aattcttggg cagaaggcat tgaaaatcct
    23581 ggtatcagcc aatctctttg tacttgattg atcagctgat ttttttccaa gtcagtcatg
    23641 attggatgca gaacatagag agactttttc ttatcagtaa gtgtgagagg tacagtagaa
    23701 aaaataatat cgtaagaagt ttcgtcaacc tcgtgtaagc ggtcagctga attagtctct
    23761 gtaaaaaaga tcatcgggaa taattgctga agttcacttt ttaagatcaa tgaagagcta
    23821 atgccacttg gacagacgat cacggctctt atttttctat ttctttcttc tgcgtccgct
    23881 tttctgatct ctccgccaaa tagtatagtg aaaaaaccaa tctcttcttc agggatgctc
    23941 ttccctattc gtctttctaa cggaagtaat gcttttcttg tcatttcgta gatggaagca
    24001 tattgatgct taatttgatc gatcatcaca ttaggtaaat aaaaagaata ggaaatacga
    24061 aaataggcag ggaccaagtg ataaaagaca ttcatcaata attcacgaaa cgaagcaaat
    24121 tctacggcca tcaggaattc catgcgatgg atgacttctg aagaacattc cagcaaaaaa
    24181 tcaagtgcag gctcctggac atctccttca acgatcgatg ctaagcaaag agagaaaaat
    24241 ataatctccg aattttgatg ttcggaatag ccaaggccag tcaatacttg tcgactaatg
    24301 tcagtgactg caagagaagc caacagttca gcatctttta ccagttccat ggatgttttt
    24361 ccaattcgaa tggttgtcaa actcaaataa taagctaatt catccattcg atttggtgcg
    24421 aatcgaagat ctgatagtga aatcgtttta tctagcaaat ctctaacttt ggcgtaataa
    24481 tcaatggaaa gggttgcggt taaatacgtg agcccaaatc ttccagcaag tgactgcatc
    24541 aaacgagcag tgcgattttt tgcttctctt cttaatattg cttctgaccc tagcagccta
    24601 aaaccagttt tcctagtata cgacagctga caatcttcca cttctaattc atttcttatc
    24661 tttttgatat ctgacaggac tgtcccttta gaaacctgaa gaaaatcttg aaaatgaaaa
    24721 acagaaacag acgttgcagt cgaataaacc atcaaataaa gaagatctct tcgctcttgt
    24781 tcggtataca cagcttcaaa tcgagtaata tccaaaaatt tttctgccca gtcgattgct
    24841 cggaatttct ttatacggat acgggtatca tccacatatt cggcaaaagg agttaattgt
    24901 ttgatcgcct cgcttgtatt tacttcgatg atctctgctg ctttttctag actgatttct
    24961 cttggtgttt tcaggagata actaattttg tagttcacag cgctgttcac ctcctactta
    25021 tattctagta tggtagcgct tttaaatcga ggggtgtttg gtacaactta cagttggatt
    25081 ttttttacga ctgtaccgaa cgatttctat cgaatggata cagaaaaaaa gcaggtgacc
    25141 aaatgtctcc tgcttttatg aattgatttc gtccgatcaa tgatgatcat gtttattttt
    25201 tgaggtcagt tcttcaaatt tttctttgac atcttcaacc gctttttcga tatctttttc
    25261 aaagtgattg ggctcattag gctttgtttc ctcttctaca gaatttgaac cagataaagc
    25321 tgtttcttta ttttctgttt caccagagtt tgtgccagct aatgattctt ctttgtcttc
    25381 tgtttcacca gaattagttc ctgctaaagc cacttcttct tctgttttag gggccattga
    25441 acctcttaat cgtttgtgtt tttcaccgtc ataatttgtc atgttggttc ctcctagtag
    25501 ttctatactt tttcatcgta caataagaca gtttatgcgt caaaaaacaa acccgattct
    25561 tacaaatatg tcatattttt ccttctgatt cttgaatgct tttgattcct tcttccaaaa
    25621 ttttcatttg taagattgtt tcttcatctt tgatgacttt ttctgcgcca ttaactaatg
    25681 tctcgtaaat cccttcgtag acacggctgt aatcaccaac ctcggaaatt actttttctt
    25741 catgataact cccttgttca tctatatagg tcaatacacc ataatgttcg ggcaaatcga
    25801 taccaaagtc ttcatgatca ggcatgtaaa acattttcag atgttcttct tgtcgatctt
    25861 ttgtttgttt cacgaacatc cctttttttc cgtaaacgac gaaacttgga cgttctttga
    25921 tgcggaaata actggatttt atcgaaattt tcatatttcc atagaaaaaa tccagatcga
    25981 agtaatcatt cattcgtcct ttccctaaaa gttgacgtac atcatagctg atatcatctg
    26041 gttcgccgaa ataagaaaga acttgatcta ctgtatgaca agcatgtcca tataaatagc
    26101 tgttcgcagt agaaaattca tgcgtatttt ctggtacttc cggtctgaaa taatcataat
    26161 gcatttctac ttctaaaata tcccctaatt ttccgctttc gattactttt tggactgtca
    26221 agaagtcaga atcgaaccgt ctgttttggt aacattggat aaatagacct ttttcttttg
    26281 ctaatgcaaa taactcttct gcttcctttg acgtttctgc aaaaggtttc tctactagta
    26341 cattcttacc atgttcgagt gccatttttc catattcata atgaaattga ctaggtgtag
    26401 tgatgacaac taattggatc tcaggatcat cccaaagatc agacagctga tcagtatatt
    26461 cgatgtcttc gaatgttttc cacactggtt cagatgggga aaaaattttt ttgatattga
    26521 ttttgttcgt aagttttctt gaaaaaggca aatgatagcg atttgtactc tttccattgc
    26581 caagataagc gattgtaagc atgagtggtg cctcctttta ttgatgtttt cagtatatcc
    26641 tagaaaagcg cagaaagtat ttataatagc tattctggta aaaaagttca gaaaaacact
    26701 tgttatcatt cggtttggtg aaattaatgt tcaaagtaag gataaaaatc aaaaagatag
    26761 tagcggaacc aagtgtttat cagtaagata gaatttggag gtcagatcat gcttattgaa
    26821 gaaaaactaa caaaacaaga gttgtttacc acgacagaga aacggattgt cgattacatc
    26881 cgtaggaata tcgaagctgc agtctacatg acgattgaag aattagcaaa agctacgtat
    26941 acttcccatt cagctatcat tcgtttatgc aaaaaaatgg gattcagcgg atttaaagaa
    27001 ttccgtttcg agctggggcg ggaagttcat caattgattg ctcagttcaa tcaaacgatt
    27061 gatcctaatt ttcctttttc aagtgaagat agcactcagc aaattgcaaa gaaaatggcg
    27121 gatttatcga tccagtcaat aaaaaaagca caattgcaga ttgaacatga agatttagat
    27181 caaatcggta aagtattgaa caaggcacag cgaatctttc tttttgcaaa aggtgattct
    27241 caaatcacgg caaggaaatt ccagaataaa atggtcaagt tgaataagtt tttgatcatg
    27301 gctgaagaat acagtgattc ttcttggaat gcagccaatt tgactagcga ggattgcgcc
    27361 atttttatta gctacagcgg tcgcatccat cattatgaac gaataatgac ctatttgaag
    27421 catgtagagg cccctacctt gctgattacc ggaaatcagc attcagaaat ggccaaacag
    27481 gctgatatgt gtcttgttat atcgcaggag gaatatgatt ttgctaaagt agcgactttc
    27541 tcctctcaaa ttgccttcga ttacgtatta aatacgttat tctcagtgat atattctcaa
    27601 aattttgaag agaatatccg caacttgaaa aacaaacaag aattgatgca aaaaggcctt
    27661 cttaaagaat atcggaaaac ttaagaaaag atcctcattt agtaataaga aagatagtgt
    27721 ttgataggct ctctttgata tcatcataaa aacaggctga agttttcttt ttcaaacctc
    27781 agcctgtttg tttacggaac gattcgaata gatatattat agaaatgagg aatagctaac
    27841 ttctgtttct tattttgaac gattgacgaa aaagatatat aagaaggagg aaaatcagta
    27901 tacatgcagc taacaatcca tacaggaaaa atcgtttgtt ctcggatact ttctgtactt
    27961 ctttttgaac ttcttttgca tgataaggaa cccggtgccc tctgacaagt aagcgatgac
    28021 tgttgaccat ataaggtgta caagttagga gtgtcacgaa atcctgccct ttttcgattc
    28081 tcagatcttt tgtatcagtc ggttctaccg tcttgatctg atcgacttga taagcgagtg
    28141 tccgtccatt gatttcgata tagaaagaat cacctaaagt cagtttcggt aagtctgtga
    28201 acaactttgc ttgaggcagt cctcgatggc tggaaataac ggcatgtgta ttttctcccc
    28261 ccgtaggata agacgttcct tccaataatg aggcaccttt ttctagcagc acagtattcg
    28321 tttcatcaaa aattggcaaa tggacattga tctcagggat cgtcaaaata ccgatcgtat
    28381 gtttctcaaa ataggttcta tcctttttcg caggttcttt tttcttagta aagggatctg
    28441 cacctggatt tccaccttct tttgctaatt gctggttttt ctttgtcatg ttttcttgga
    28501 ttttagccat gacttcttca ttttctttga ccgcttgttg ctgataatga gagatgatct
    28561 gctgatccag ataattattc agcgcgtcgc taacaaatgg ataagctagt atccctattc
    28621 ctgagagaag caagaaaagc atcagcagat ctagaagttt ccttttcttt ggtttcattt
    28681 gtgttctcct tgtgatgact gtttgataat aaaagaaaaa ggagcatttg cgtaatccgc
    28741 tgctcctttt tccaattctt gcaaaaagac taaatctgac tgtgcttgcg tctagcaaag
    28801 tacagtccag caatcagtag aaggactact cctgcaccga tatacacata gattccctta
    28861 ccgcctgttg aaggaagtgt acctttgtgt ttatttggta ttttttcagg agaaatcaac
    28921 tgtcctgctg ttacataaga ttgttcatcg atagtaaatg ctacacggtt tgttaatgga
    28981 acatattttt ctggcgcttt cgtttcttcc agatagtacg tgccatattt tagacctgtc
    29041 acatcgatta aaccgttact tgtggttgta aaaaccgttg ctgattcttt cgccgatacc
    29101 cagctgacgg cttttgtgga tgggtcgatc gataaatatt tcgctgtgtc actatcttga
    29161 tcacgaacga cgaattctgc tccagcaagt gtttggtctg atgtaacgtc accatctact
    29221 ttaacaaatc gtttgccccc agtaacgaca tcgactgacg gtggtgtttg atcatttgta
    29281 tgcccgttat cgacatttgc ttggttagaa aatcctttgg ttggatctgc tttttcgttc
    29341 aaatgcatat agtaaacgaa tttcaatgta ccgccaggag ttaatgaagg aatataattc
    29401 ggatcaactg aaaccgtgaa tccgtctgtt tgctcagtga cagaataatt tactgggtcg
    29461 atttctttat ttccatcata taaggcataa gcatacgttc ctgaagaatc attatcaaat
    29521 gttaaagcag cgtcatgagt atcgataagt ttgaatgttg tgtacttgtt ttgcgttcct
    29581 tctttatcag cgatacctaa tgggatattg acagaaatct catattgaat tttttcaccg
    29641 attgcgacat cttttccatt caattgaggt gttgttttat caactttaga cgtatcattt
    29701 ttgatggtct tttctactgg tgtttggcta tttgccagga tctcaaaagg cgtgattgtt
    29761 tgcttttcaa tcatttccgc attatcagga gcttttactt cttctaaatt atattttcca
    29821 acttctaaat gattaacgat caactggcct ttttcaatag atgcctcggc aaagtcattg
    29881 ttgccgatgt cataagaatg accagtaatg aaatgtttgg ctttggtttg atcagttgtc
    29941 caagtgtaca atccatctgt gacactttgg atgtattttt tgacgcttgg tgttccttct
    30001 tctttagaaa taataaattc tgctccattt agtgcttcgt tttcggcagt accgattttt
    30061 gtaactttca acgttccatc attacagact gtatttttag ggtagagatg gatagtatct
    30121 agttcttctg tcccgtattt ataagagcca tctgcttgtt tgatcatctc atatacaggg
    30181 aaagctaaaa ccatgtttgc ggcagctgac actccgtctt ttggttcttc tttgatcgtg
    30241 tagactgcat ctttcccatt ttgtttttta ggtaaagata aagtgacatt tccatcagca
    30301 tctgtcgttc ctgaagcaac tggtgtacca ggagtcaaag actggactgc ttgttttgct
    30361 gcatccacgg acgctccttt atctcgttgc gcataaaatt cttgagtgac gttataaact
    30421 gaaaatgaaa tatcggctaa tccttggtat tgatcgaatt cgctcatttc tttcccgctg
    30481 ttttggatta aaggatcggg taaatcagtc attttctttt tatgaaggat gacttgagct
    30541 gctgtctcct ctgcaaacac tttttttgcg ccgaagctgt ttgtgagtaa tggtaaaata
    30601 aggaaaagga ctcctaacat aacgtttatt tttttatgat ttttcatttt ttcactcctg
    30661 ttcttttttt gataaacaac cagattgcaa agcttgcgaa taccatacca acaacgccta
    30721 aaagtgtatt tttcgtttcg tttgtttttg gaagaaatgg acggctattt tcttttttat
    30781 tgactacttt caatatactg tccgcctctt tatcctcaga aagaaaagtt gaaatggtaa
    30841 acggaatttc tgtttcactt aaaatataac cagaaggtgc ttcgatctct tttaatcgat
    30901 aacttcctgt tttcaacccg gaaattgaaa aagcgccatt tttatcggaa atcaggtgaa
    30961 gcgcatctga ttcgttcttt gtccacttgt atccgttggc tgtttgctgg aggtaatttc
    31021 ctgctttgtt tttaataagg aatactgcgc cagctaatcg tttatctggt ttatttgtct
    31081 cgattttgac aaattgctta ctccctgtat atacagagac tttctttgta ctgacaccaa
    31141 aaccaaaatc cagtgttccg ttgttgttga tttccttgtt cgcctgtgct gtactgctta
    31201 gctgcatctg ataagaaatg gtcagttttt ttccagcata tttttccaac gaagggatcg
    31261 aaaataaaac atcaaatccg tgaggtttct tgtgtatgac aaagccttct gtcagctttt
    31321 ctccattcga tgaaatcgtt aaactattag gtaaaaacgt taatgcttca tccgcactat
    31381 ctgacaattc gaacttttga tagtcaagga tatttacagg aattgtcgta tgcaaggaat
    31441 aagtgatttt ttcaccaatc gtaaaatcat ttctaggctc ttctaatacc ttctcaaaag
    31501 gtggatcagg gtagtcgttt tcttcatttt taggatacaa atggatagtt gaaagttttt
    31561 gattgttttg tccatgtaca ggaagaacaa ctaccatatt ctctgccttt tctttgacga
    31621 cttctggtac tttggattca atgaaaagat acgctttatc tcttttttct tgatctttag
    31681 cggccagaga aaaagaagcg ataccatctt cgttgttaag agttgtagtt gttgcttctg
    31741 cggtaaacat accggaagac gcaccgtttt ttgcgatctc tgcttgtgct tcttctaccg
    31801 ttttgccctt ttcccgtaga tggtaaaaag aatctgtgac atcataaact tggaatgtga
    31861 caccatttaa tcctcgatac gtttgtaata aagctttttc ttgtccgtca tttggatgat
    31921 ttttcggcag ttgcccgttt gggaaaagca atttgtgtaa agtaatctga accatttctg
    31981 tttctgtttc tgttgctacc tgagtaaaag ctggtttaaa tagtaacaag aagagacaca
    32041 tactaagcca accaagtttt ttcatattta gctgccccct ctttcttttt tcaaaaaaag
    32101 ataccaaata gaaaacgcac aaccaatcat ccctactaga tagattccta aacggccaat
    32161 tcctcccgtt tcaggtaatg gtacttttgc ctgattcgtg atgtctaaag aaatctggtt
    32221 gtttttggct cctggtgaca gaacagattc atgatcttgt ccatccactt gaattgaccc
    32281 atcttcgtgt ataactatag taactggctc ttttagacct tggtatcctt ctggtgtaaa
    32341 agtttcggtc agcgtataag ttccaggttt taaattttcg aatagaaagg tatctgtttc
    32401 ttgtccatct tttggcgatt ctaagtccat ttctggtccc tgcagtctga attttgctcc
    32461 ttttaatgtc tgtccgttgt cttctttttt gtgtaccgtt agatcaaacg cttttagtcg
    32521 attttgatga gtgagcgtcc atacattatt tgtcatctcg gtattttttc ccgtgtaaat
    32581 gatttttcca tatttatcga tttggaattc ataatttccc ggcgaagtat catatccggc
    32641 aggaccagct gtttcaacga ctcgatattt accaggtgtg agtgaatcaa aactggcaag
    32701 acctttttca ttcgttgttt ggctgtcaat tggctgataa gtaccatttg cttctttttt
    32761 ctgtagggaa aatgctgctc ctttaagatt tatctctttg tccgtccctt gcatcgcata
    32821 cttacgtact gctacaggaa cttcaacaaa aggattttca atagtcaact ggatcgtatc
    32881 atcggaagta gtgactgttt ttccatcaat ggttaccgta ccatcagaag cgatcttgat
    32941 ttcccaagta gtctttttgc ttaatccatg cccttctgga gcttttgttt ctgtcagcgt
    33001 ataggtcatt tctttttgca atttgacatt ttctggaaga gaataggttc cgtcgccatg
    33061 atctgttagt aaagtatcaa tagaatctcc tgttaatttg aaaacagcgc caataagatc
    33121 cttttcacct gtagaggaat ttttcgttat tttcaagttt aacggtgtga attgtttagt
    33181 atttttccat gtcattgcat ctatttgttg agaatcgtat cctggtacag gtaattcttt
    33241 gattgtttgg taactgaatg cttgaccttg attattgtat tgaggtagtg ataatatctc
    33301 ttgataactt tctccgctat ttgcagataa tttgtcaatg tctgcacgtt cccacgtatt
    33361 tgttgtatct ttagctggtt taatgattcg aatatatccg tttttccaag ctgcagcatt
    33421 tgtcgtatgt tcccgttgaa tctcaaaagt aacttgatct ggacgatcag ctagattgtt
    33481 gtcaaattct tcccataact ttttgatgtg aagactgact ccgggagctt tagcagatgg
    33541 tataccgaat tcagctaatt cattggtatc aatgtttggc tggaaagttg tcctgccgtt
    33601 catttgatac cagaaatttg gatggaagtc ctcattttct gtttggattc tcacttggta
    33661 atggatttgg atttcttggt cttttcctaa atagatctga ttgctcttga tggtatttcc
    33721 ttctatggaa atagatggag tgaccgttgt aggacttgtc cccacactct tgactgaaag
    33781 tgtaccaggc tgataaatga atggttctgc gattggatca tttatttgtc cattgcttac
    33841 agttgctgag atctgtacag cttttttggc taggtattca gagatatctg ttgcatgatc
    33901 agctgattca tagtaaagat cgcctttttc atctgatgaa accatttgtc gcatacgaga
    33961 ctctacttct gcttttgaga gaccagctgc cggatcgctt tgaagttgga tgccaagacc
    34021 atgtatttcg attcctcgtt ctttgagtgc catcgcttct ccgatggttg cgataaacgt
    34081 actgtcgatt cttctggata gattgttttg gtcaggtgca tcatagattc ttgagattag
    34141 agaagtattt cccggccgat cttgcgtatt agaaaactga gttccgtaat aattgctgct
    34201 tgattgtgcg tgtacccgct gtactttata ggaaaatgtt ggtacaccat ccgtcagcaa
    34261 aacgatcact tttttatgtc catttggaac ggatagcatg cttcctgcat ctcttagtgc
    34321 tttttgtgta aaagtaccac catttgtccg tgaaggtgta atggatttta cttgattttt
    34381 cactgaatca aatgaaccca tctgtactgc accgttactg tagctatatc cttcgcttga
    34441 gtagccgaca tatcccatat tgattttgtc tgtgataccg ctatctgcta aagtatcgac
    34501 aaaacgatcg acaccaatct ttacttcacc gatccgatta ttgtcgttca tacttcctga
    34561 ccagtctacg accaatacga gatcaagagg cgtgatatct ttttgtacat ttccacgagc
    34621 attcaaataa acatcaaaca atccgggtgt acttgtttcc tttgcatact ttcgtatcgc
    34681 atagtctgct tgattaccgg ttcctccata ttcgatatac gaatgggtcc gatcattagg
    34741 atctccattc caagaattta taccatccca ttgatttgtt ccgtttttat tgccttgatg
    34801 atcaagaaca tttgtatttc ctgtaggttg ccaactggct ttaggataag tgccctgatc
    34861 atctgttgta tattctgtag gaatcgtttg attagatggc acactcacag ctggcaagga
    34921 taatgtaggc aatgtaaatg ggctgacaga attttcgttt tcttttgatg attcttcttc
    34981 tatttcgtct ggctttggta ctgacacatc tgtatctttc ttattttctg tctcatcaat
    35041 aatgtcatat tctttattat cgacgatcag cttgtttgcc tggctgtggt aaagtgctgg
    35101 atcgattgga aaactcagat cgatagtttg tttagtgttt gatgagattt ccaacgtaat
    35161 tttcccgcta tttacctgat aagcatagcc atctccttct tttctttcca catcacttaa
    35221 gtaattttcg actagaccaa tttctttttc tatcttttca gcactggtgt tattcgcatt
    35281 tagcttgatg ttcagtgctt tttttccaga ctgatccgaa atgacttcag ctgaaattgt
    35341 ttctaaaact gttggatcat ctgtagtgtc tgctattgca gataatggca caaagtttga
    35401 taaactcaat attatcagca tgaaaataac tttcagtttc ttccctgtgg tcgtcatttt
    35461 tcccctcctt tttctttttt tctgttttga cgtgaatagt gaaaattatt aaaataaaca
    35521 ggcgttacat aattttattt attttaatat gataattatt atacgaaggg cgctaaaggg
    35581 aatcacacca attttcatac caattggttt catttatgaa gttgattcct tcgattaatg
    35641 agcgtttcat tgaaagaaaa gggttgaggg agaaaaacat atgatcaggt tgttggaatg
    35701 caactataga aagatggaaa aaatcattcg aattctgtcc atttctgaca agtggatgac
    35761 taaaaaagaa cttgccaaac gaattggtag ttcagaatct acatttgtaa ggtatatcga
    35821 agagatcaaa atccgctggg gaacggatgt aacgattcaa tcttcacaaa aattggggta
    35881 tcgtcttgaa catttgaatg taccagctta tttaaatgtt ctgatggaaa tgtcgcaaag
    35941 ttctacaaac agtcgtttat taaatgagat tattgtcaat ccgggtaaaa cgattgagta
    36001 ttattgtgaa aaaatttcca tcagccggtc ttcttttaca cgtaagttga aacaatgcaa
    36061 tcaaatttta gaaagctatt tattaaaagt gatcgttgac caaggttttc agttaactag
    36121 ccttaaaagt gagctccagt tgcgtatctt tgttaccttt ttctttttaa gttactacgg
    36181 aagacgcacg ttgccatttc caatcgaaaa aaagaagatc aaagaaatta tggtcaagaa
    36241 taattgtcag atgaatttgt ttcaggaaga aagcccatat gagcaatctt tctttattat
    36301 gtattttgtt gtgcatctca tgagagaatc ccaaggaact ccatttgaat ggaaagctgg
    36361 agactcttat gtacagttga taaatatcga taaaaaagac ttcttaatct tgaaaaatct
    36421 cttaggaaaa ttgaagtaca gcagctatat aaaagtagtg aactattttg tcaatacgat
    36481 tttttcagaa ttttcggaga aagaaaaaaa tgaaatcagt gagcagatac gaaaaaatat
    36541 gtcctactgt tcgttttcat ccgctgattg caaagaaaaa aacgaagaat atgttgttca
    36601 attattaacg caaatttatt ttttctcccg tattttccca tacgatacga ctcatttgac
    36661 gatccgtgca acttactttg cacagcagat gcaacaccgc cagatacgtc tatatgctgg
    36721 atttcgtgca gaacttcaat tttattccac tctactgaat cagaatctga tttattattt
    36781 atctagctat atcttttgga tgcttatcgg taatcctgaa attcaccatt atacttctga
    36841 taaaaaaata ctgattatca gcgacttaag tcttcgacat agtcaatata ttgaagaata
    36901 tatcagtgat attcttgctg tgcataaaat tcactctgag acaactgcta ttactgaaga
    36961 tcagctgtcg aaatacaatc ttctcgagta tgatctgatc gttacgaatc aacccatttt
    37021 gaatgcccat gttcctcata tattgattga tgatagtgtg tcattcgcaa atgaagaaga
    37081 actcatccgc ttattcgagt tatgaataat caaaaaacag ctatgtataa accaccctga
    37141 taaaaatttc aggacaaata cacatagctg tttcttttta ttgtattcct tcagcaatcc
    37201 aacgagattt atagcgaaag tcttttaaca actcttcttt tgtccaatta tttgcaatga
    37261 gatccgtcaa agtgttcatt acgagggtca tggaaaagta taggaaacta actgcttctt
    37321 ccgacgattg aatggagaaa tgattgattc ttaataattc cagccatttt tcgttcattg
    37381 gagaatcagc cgatagttct ctacgtttgg ttgtctgctg atcatttcgc tgggttaaca
    37441 agtataacct ttgccaatag atactttcgt gatctaagtc gcagcaccac tctaaatatt
    37501 tttcgatccc caaaaagaaa tgttgtttgt tttcgctaat gaattttaag atatcttgat
    37561 ggactatgtg agaactgctt ttgatcgtat acaaataagc atcttccaaa tcttgaaaat
    37621 atttataaaa agcaccgcgc gacatcccca tcttgtcaac gatttctgag actttgactt
    37681 ggctgatgtg ctgtgagcaa aatatctcca gtaataggtc atcgattctc tgtttctttg
    37741 cgttcgataa attgaaaaaa gtttcttttg gcatccacat ctctccttta tctctgatta
    37801 tacgtggata gcccttcatt gtcatgaagg attcattcct tctttttctc ttcttctgtc
    37861 aagtacccat cttccattcg ataaacgcga tcactccatt gaatcatacg gacatcatga
    37921 gtaaccataa tagtcgcttt ttgtttctca tgagcttctt taactagtaa tttgacgact
    37981 tcatatgcat gatctgtatc caaactagct gttggttcgt ctgctaagat caagcttggc
    38041 tcattatata aagcgcgagc aattgccact ctctgtcttt ctccaccaga aaggtcttta
    38101 ggataactat ttttaagtga ctggatatct aaagattgca gcaaggaatc gatttttgtt
    38161 gaatcttctt tttttgaagt aacttggtca actagttcaa attgttcctt taccgttaaa
    38221 aaaggaatca aattagagct ttggagaata aatcctattt ctttaaagcg taaatctgct
    38281 cgtttttttt ctggaagctc agttagtgag tgaccgttga tcactatttc tccggaagtc
    38341 ggtgtttgca atcctcctga aatggtaagg aaagtacttt tacctgaacc agaaggacca
    38401 atgatgctga cgaattctcc ttcctttaca taaaaatcaa ttgctttcaa agctgagacc
    38461 tttgtatgac cagaaccaaa tgttttggta acttttttca tcgttaatat ttttttcatt
    38521 tttattaacc tcctattgct gtaattgggt ctactttggt tatcgtacgt atcgaaaaca
    38581 atccgcctag cattgcgaca atgatcaaaa tcccactata tagcaaccat tgtgaccaaa
    38641 aaacagcaaa aggcatcgcg cttggtaaaa tcagactagt caaataagca aacaaaagag
    38701 caagcagaac gcctaccacc ccaacaataa aggactgtgc tactaaagat ttagcgatga
    38761 agaaattttt gatcccttgt gctttcatta ctccaaaaat agcagtcttc tgaagtgtca
    38821 tcacgtacat gaaaataccg accacggcag caaccactaa gaacaaaaag tatatcatcg
    38881 catttaaggt catattttgg gcagaatagc caggaatact ttcaatgaat gtcccaatag
    38941 ttaataactg tagacccttc gatgcttttt ctttcgaaat tttagctgaa tcctttatta
    39001 cgatcccatt gatcggctgt tcttcttcag aagcaaacgg ctgttgtcca tacttcaaag
    39061 cagtccacgt atctagggac gcatagatga cgggagaaac tgtgtaataa gtttcaggaa
    39121 agataccgac aatcgtcaaa ggatcgtcgt aactgccaat tttgatcgta tctccaattt
    39181 tatagcctga atcagccaga ttttgagaga tgatgatttc attctttgtt ttaaattcat
    39241 gccctttcgt gagttttggt aataaaaatg cttctttcgt tgtgccgaaa actgtaacat
    39301 tttctttgtt ttttcctttg attgctccgc tgtacaatcc gattggtgct ttctctcctc
    39361 cttcaatgta tttcagatct cctcttgtta attgtgaagc tgcaaatact tgatttgcat
    39421 cctccgacaa aacaatctct tgtgcgtccc aatcatcgat tgctttttta aactcttctg
    39481 aaagtccatt cgctagtcct gacaacatga atacgacata cgcaatcaag aacataatac
    39541 cgacgatcaa gccatatcgt aatttagaat agcgaatttc tttgatagct agaaacattt
    39601 gtttttcctc cctatgatag attgatagtg acaaaatgtc acttgttgaa tagtgtaact
    39661 ctaaagtgac aatttgtcac ttgtgttgtt tttttcttat caataaattg tctaagacat
    39721 ctttcgataa aaccttaaca tgagtcataa aaaatttatt taagccttat ttatgctttc
    39781 catgataaac taaagtgacc aaatcaatga ggtgggaaaa tgattaaact attagtagta
    39841 gaagacgata aagtattatc caataatata aaagaaatac tcgctgcaat cggcgagata
    39901 acacaagttt atgatggtag tgaaggactg ttcgagttgg aaagtggagt ttatgatctt
    39961 gcaatcttgg atttgatgat gccggaaatg aatggatatg aagtgttagc aaaaatacgg
    40021 aaagaaaaaa tacagactcc agtattgatt ttgactgcaa aagatagttt ggaagataag
    40081 attgaaggat ttgaacgagg tgcagatgat tatctaacaa aacctttcta tcgagaagaa
    40141 ctgatcatgc gcgtgaaagc attattgaaa cgctcgctag gtctctttga tgaaaatgtt
    40201 ctgaagtata aggatatcag ttgtgattta aaaagcaatg aagtcttgta taaagaacaa
    40261 gttttgccca tacaaggaaa agagtttgaa ttgctcgttt attttttaca aaataaggga
    40321 atcattttga caaaagagca gatatttgac cggatctggg gatttgattc tgatacgacg
    40381 attacagtta ttgaagttta tatgagcaac ttacgaaaac atctgcgaag tactggattg
    40441 gataaagaaa tcaaaacctt acgtaatgtc ggttatatac tgcaaagggt gtgatgcaat
    40501 gaatggagat attacacgaa agcaacgtag tcgctttttt gcgacaaatg tagcagcatt
    40561 tgcttttatc ttttttcttt tgggattcat tactttacag atattaaacc aatctgcata
    40621 cagagaaaca gatctgtcat tagagaaaat aaattttgat tctcgaacag tacaattaga
    40681 gatacaaaga tatcagcaag gcgatccctt tttaaacccg actcaaacgg aacaaaacag
    40741 aacaaacatg gaacagccat taatggaacc atcaaatgac cgttttaaca ctcaagtgat
    40801 cttgtggtct aaaaatggtg agatattgaa taaagcagct attggtgggc gtttcaacca
    40861 aatcaaagaa ttaaaaatgg atacgaaaaa tctgaatacc atccaaaata ttgaattaaa
    40921 tgaagacagt ggctcatctg ccctctcttt ccgttcgatt acgaaaaaag cggagacgaa
    40981 tcaagatggc gttgcctaca tccaaatttt agaaaataca aatcaaatca agaattcatt
    41041 agcgactttt cgcacgatta tgattttgtg tatgattgtt ttttggattc tctctattgg
    41101 tatcagttat tacttgtcta gtctaagcat gcgccctatt attctttctt ggagaagaca
    41161 aaaagaattt gttgagaatg cttctcatga attgcgcaca ccgttaacga ttattcaaaa
    41221 tagtttggaa catcttttta ccaagcctaa tcatactatt attgaagaat ccgaaagcat
    41281 cgctcaagca ttgtcagaga cgaggcgcct cactggattg acttcggatt tgctgacaat
    41341 cgctcggaat gattctgatc agcagctgct ttcaaagcaa atgatcaata cacaagaata
    41401 tatcgaacaa cttgttaaac catttcaaga gatggccatc atagatggaa aagagtttat
    41461 tctccataac gatgcaacat tagaagtacc aattgatgaa aaaaaaatcc atcaagtttt
    41521 ggttattttg ctggataatg cattaaaata tacgaatcca gaagataaga tcattttgga
    41581 ttcatctgtt aaccataaag aatggctgat tcacgtaaag aatactggcc cttctatcag
    41641 tgaagaagat caagcaagga tttttgatcg attttatcgg gaagatcaat ctcgggcgaa
    41701 agagactggc ggatacgggc ttggcttggc aattgcaaaa caggtaatcg aacagcatca
    41761 tggacagatt tctgtatcca attatcttcc acaaggagtc gattttaaag tccaattgcc
    41821 gttgaagcct aaaaaataat gcttatccct aaaaaagggc tgtgacaaaa atcttgtcac
    41881 agcccttttt tgttgcgaat aaacggtgtt ccagaagtag cttcttcgga aataagccga
    41941 aatttcttta agctgaccac ttctgtcaca acctctttag cttacgctta cgatttttta
    42001 ttgcggtctt caatgtgtac atcaccgctt gtgtcaatat ccaattcttc tcggctaaca
    42061 tcttctgtca ctttttcaac atcttcatgt tcttctttat gaatcccaac ttcttcacga
    42121 ataactgtat gtttgcttac atctacttgc tcttctttga ttgggatagt aatcgtgtca
    42181 tcttccaatt tttcgtctgt tccctgtgaa gattgatctg ttactggttt gcgttcgatg
    42241 acgatttctt cacgtttgac cggtacttct accgtttcag tatcgttgac cacatgtttg
    42301 tggatgtcga cttctccagt tgtcacatca tgtgtattta catctaattt ttcttctttt
    42361 agttgaattg tatcatcatc tagatttggt ttgcgatcga tgtcttcagt cttgcttgta
    42421 cccattgtat cagtctcgcc aggtacgtcc atcggatcag ttcctggtgt catattcaat
    42481 ggatccgttg tattttgaaa gcctgtcgtg ccacctacgt ccattggatc tgttccagtg
    42541 ccagtttcta atccagtccc cattgtgctt gctgggctaa cagtatcttg tgtatccatt
    42601 gtatcttctg ttgtttctgg ctgacgatag ccttttactg tgatgacaaa ttttccatca
    42661 gaaatatctc ctctatattg atataaagga tcattttctg gtgtagccgt ctcagaatca
    42721 taggtatcaa aagaaaaagt atcttttatt ttttcccata aagaacggtc ttcttcacca
    42781 tccatccgat tttcatctgt ttcaacatct ataccagaca atccgtcaaa tcctagtcta
    42841 gatgctgctt catcagtagt ataaataacg atatcatctc gttcataccc ttcgttacgc
    42901 aaccgttcaa ttactgataa tgcttcttcc ctcgtactat agcttccgat gaccgttaaa
    42961 tttttctctt ccatgtaaat tcctcctagc aaatagattt atgattggta tattgttact
    43021 tataaattta ttctatagga gacgattgtt ttctacaaat atttagattg gaatttgatt
    43081 aaaacaattt aattgaaatt tgaaaacatt tatgttaatg aaagtcatat tctaataaaa
    43141 tgtcatttgt ttctatatga atttttatga aaaatttcat ataatttcca ctcgttgtga
    43201 tggtttaatt tatccaatcc atcaataagc aaacaactta tataaaaaag gattaattct
    43261 caatataatt gctcctacca tagaataaat cttcatgtaa agtaatgaag taccaactat
    43321 ttctaatact tctttaagaa ttatcccaac aatatcgtta gacaaaccat tattgaataa
    43381 atggagagcc aatcgattct gaaaaaatgt tttctaattt ttcggaaata aaagaaagag
    43441 cctcctgcca ttagaacgaa ccaaatccat ttcattgctc atgaagtaac cacatagcta
    43501 aaaagaggat aaatcaatta aaagatcaaa agaatgactg cactaataaa ataactaaaa
    43561 atgtatcttc ttacttcata tcttttagct agtacttagt taaaaatttg cttcctaaga
    43621 tcaagacaaa ctgtctatct tcatttgatg aaatgcggta aaatatagaa aatgaacact
    43681 attggaggga atttgtaatg gacagcaaaa ctagaaaaca actgcaagaa aagttaaagc
    43741 aacgtacaca cttgctccct tccgtctttc aacagttttt agaagaaaat cgttcttccc
    43801 tttcattagg cagtcgctat gaatacgcaa aagattttca tctgtttact gattatttga
    43861 gaaaagtatt ttctgaagaa gtggatgatc agctgattat ccatttagaa aaacaagatt
    43921 atcagaattt cttagctaaa atctatcgac atactcgttc gttccaaact acagcgaacc
    43981 aagaaacgga gcaattgttt gaaaatagct attacgggat ttcaaggaag caatattcat
    44041 taaatcattt tttacgattt ttgtatcaaa aaggactgat tgaagaagag atttctcttt
    44101 tgggtacaag tttaccggaa acaaccaaag cagctccgcg atatatcgat gcagaacttt
    44161 tcaaagattt tgagcatttg tttatcccga ttgaaggctt tcaaacaagc agagaagcta
    44221 gctttgaaga aaagaataga ccgaggaacc ttgcaattac tgccattctt ctgtattgcg
    44281 gattaaagat ccatgaagta gtagaactta aacgtaaaga tgtatctttg accaagcaga
    44341 ttcttaatct gaatagaaaa gaaaacagac tgaataaagt accaatacca aaagaagcga
    44401 tgccatttct ggagaactat tttaagttaa cagcacaaaa tacggacgaa aattcttttg
    44461 tttttcgttc ctcacatgat cagcaaattt ctcctcggac tattcgccaa attttgagca
    44521 aaattacagt atataaaaac gtatcgccag aactatgcag aaaaacatat cgttattatg
    44581 cagaactata tttagatgat gctgacgcgg taaattattt atgtggtaat cgatacctct
    44641 catcccattc ttttcaagat atctgggaaa agatggttga tttttcttat cacgaattag
    44701 cggcattcgt tcgggtttct tccatataaa acaaatgtat gatctttttt tcacaataaa
    44761 ctatgttatt atataaagga ttatgttacg attatcttga atttgtatat tacttaacaa
    44821 aagataatgg agtgtgaatg atggagacca taacgtattc ccctttgaac ttatttacga
    44881 attttcaaga agctgcagct aacttttccg agaccccgat tatctttgat gagccacttt
    44941 cagctttccc agaattagga cttgaaacaa cttacaaaaa atgctgcgaa agtgtgttga
    45001 aacgggctta tcaattggct catcttggag tgaaatccgg agacaaagtt attatttata
    45061 agagttccgc ttttgacaca taccttctag ctgtttctgt gtcttacctt ggtgcagtgc
    45121 ctgtcatgac ttcttatcat ctaccaacaa caacaatgga agtatttatc gatcgtttag
    45181 aagacccttt cattttattt gatgatgaga caaaaaagcg agtgagagaa atttcaaatg
    45241 gaacaaaatc aaaacagatt cctattcttt atttaatgga acaacctgct ttgtctgttt
    45301 ctcaatcatt tttagacaaa aatgaaattt cttatatgac ccatacttct ggtacaactg
    45361 gtatccctaa acttatttgt cattctgctc attctatggg atggcggacg aaatggcaaa
    45421 aaacagtttt cacaaaaatt tcggaaaaga aattaatcgc ttttcacatc tctccagtgc
    45481 attccagatt caatattggt atctcttctt tgatgtccat gggatttcct atgatgcctt
    45541 tagcaaacgc acaaagttca aaagttgttc atatgttgga agctcaccgg ccaattgcac
    45601 tagagaccca tccgaataac tttgttcagt ggcgttttac tgctaaggag catccagaag
    45661 ctttttcggg tatccgatac tatcactcca cttttgatgc tataaacaac cagacaatga
    45721 agacattttt gcgaacgtcg atggcacagg atgcggtatt tcttcaagta tatggccaaa
    45781 gtgaatgcgg accaatgatt cttaaggctc atacactaga gagtttgaaa acaagtgatg
    45841 caagagatat gggcgttgga ctggaagaca tgacaagcgc tcggattaca gattcagttg
    45901 ggaatgtcct acctgaaaac acagatggtc atatacaatt actgtctaaa ggacgggctt
    45961 tgacttatta caaggaagat gcacggttcc aggaaaatgt ttatggcgat tggtgggata
    46021 gcggagatta cggttttatg gataagcatg gccacctatt tttgaaagat cggcaagtgg
    46081 atttgatcga aacgatcaat agtacattag ctattgaaga ctttttatta gattcgttag
    46141 attttttggc agaagtagtg attgtaaggg ataaaaatca ttctccacaa ccgattattg
    46201 ctttagctcc tgataaagaa atggattgga atcgatggtg ggagcaagtc catgaacttc
    46261 ctcgcttgaa cgtccctatc atccgtgatt ttgatgcaat cccgcgaaca gctacaatga
    46321 aagtgcaacg gcttcaaatc gagaaagaat taaaatcaca aaagtcctaa ctatttaagg
    46381 aaagaaagat ccattttggt ctttcttttc tttttgtttt tttaaattgg atgagataaa
    46441 aatatgccaa ttggctgttt ttcttttttg aaaatttcgt tatactctta ttcatggagg
    46501 aaaatgttat gaaaagaatc ttgacaattg gcggttctga tccatttgct ggtggaggaa
    46561 tccaaacaga tttaaaaact tttgagaatt ttggtctatt cggactaagc gccttgactt
    46621 ctataggggc actagatatt aatgagcgat ttatgctgga atctatatct ttggaattgc
    46681 ttaaacgaca gttagtttcg attgataaaa tgacttcatt agatggtatc aaaattgggt
    46741 tattgaattc aagtgaagca atcatggttg ttcgagactt cataaaaaca aaacaaaata
    46801 tcccaatcat tttagaccct gttttggcat tcaaagaaac tgaagctgtg atcaatcaat
    46861 tgtatatttc gcagctcatc gaagaacttt ttccacttgt tacgatgatt acgcctaatt
    46921 tggcagaagc tgctttactt tctggtcaag ctcttccctc ttcgttaaca gaaatgatcc
    46981 atctcgctaa agaactcact gcttcaggaa caaaaaacat tgtgatcaaa ggcggcacaa
    47041 ggatggctgg agatgaagct gtggatcttc tctattcaat tgatcattat gaggtatttt
    47101 ctcataaaaa aatcgacact acaacagtaa acggggccgg ttgtgccttt tcttctgcaa
    47161 taacagcgaa tcttgtgtta ggggcagatt ttaccaaagc agtagcagaa agtaaaaaat
    47221 ttgttgccca ttgtatttct aatggcgtgc tgatgaacga tcataccgga agtgtctggt
    47281 ctggcggtat tcaaaaagga ggaatgagaa atgaaaacta agcaattaac gatctatgct
    47341 atgctgattg cgttgacagt tgccctttca ttaacaattt tgatccctgt accggctacg
    47401 aatggatttg tgacattatg cgaggccggg atctataccg ctgcttttct ttatggtcca
    47461 acgggtggat tagttgttgg tgcttccagt ggtctattga tcgatttgat ttcaggatat
    47521 ccgcagtggg cagttttttc attagtgatc cacgggttac aaggatatgt tgctggttct
    47581 ttcagcaaat cttctactcg tatgtgggga ttagggcttt tattaggaag cttagttatg
    47641 atcatcggct atggtatcgc cggttggatt ttatatgatt ggccggctgg agtggcttct
    47701 ataccaggaa acatcttcca aaatctgatt ggaatgggac ttgcctttcc tataactgca
    47761 agtcttcaac gtattcaagc acggaagcaa tattaattga aaaagaaggt tttagaaaat
    47821 gaagttgtca gaaaaagaat taaaagacca attgactgaa atagttaatg acatattggc
    47881 tgaggcacac ttgaaaaaag gtgatctttt tgttttagga tgcagcacaa gcgaagtcgt
    47941 tggaggacat attggaaaaa attccagtgc ggaagttggt caatggatca tccgtacgtt
    48001 aaaagagttg ttggatccta aagaaattgc gctagctgtc caaggttgtg aacatttgaa
    48061 ccgagcatta gtagtagaac gaacagtggc agaagcaaaa aatttcgaaa ttgtctctgt
    48121 tgttcctgca ctacatgctg gaggtgcttg ttctgttgct gcttttgacc agttcaatga
    48181 tcctgtcgaa gtggaacacg tagttggaca agccggtatc gatatcggtg acacatcaat
    48241 cggtatgcat atcaagcatg tccaagtacc tgtcagacct cgtttgaaaa cccttggaca
    48301 agctcacgtg acagcccttc gctctcgtcc taaatatatc ggtggtccta gagcgaatta
    48361 ttgaaatgag taattgacag agcaatctga aaaacttcct cgcaaagtcc aactaatgaa
    48421 ctttaacgag gaagttttac ttgtttgttt tatataaccg agcttcataa ggtgctagta
    48481 tgcctttagc ttcaaattct ccgctagagg aatttgacaa gagtaatgtc caggactgat
    48541 ctgcttcttt tggaagttca tagtcagcaa gtgattctgt taagtttacc ataataagat
    48601 attgttcatc ttctaaatat ctttcataga cataaagttg tggatgttca tgaagatatt
    48661 ctttataaga accgtatatc agacctttat tggtttgtct aatgcggatc atgttcttat
    48721 agaattgcag gaccgactct ggttcatatt cttgttgttc tacattgatc gttttggtat
    48781 ttggattagt gatcagccaa ggttcatgtg tagaaaaacc agcatattgt tccgaagtcc
    48841 attgcatggg tgtcctgctg ttgtctctcg ttgtttcacg aatgatatcc aatgcttctt
    48901 ctcttgtctt accttccgca agcagccttt tataaagtct tttcgaatct actgcatcaa
    48961 cttgatcgat cgattcaaag ggcatattcg tcattccgat ttcctgtcct tgataaataa
    49021 atggcgtacc ttgcaaaaag aaatacatca tagcgattgc ttttgcagac atagcccaaa
    49081 aatcgggacg agtatctcca aacactgaaa cggcacgagg aacatcatgg ttttccatat
    49141 ataaagcgtt ccatccgata ccatccaatg aagtttgcca agcgcttaat gcatgtttca
    49201 atttaaggac atctaattgc ccttcttttt cttgctgcca gatatggata tgatcaaatt
    49261 cgaatatcat atcgaaataa ccgtctttcc ctacccattg tggtccttct tctgcagtga
    49321 caccacttgc ttctccgaca gtcatgatat cgtactcttc aaaaacatgt ttcaaatcgg
    49381 tcaaatgttc ttcgattcct gagacatttt gaaagggtga gaaagggttc tctgtggctt
    49441 ttactgaata ttcatctttt tttacatgag aaatcgcatc taaacgaaat ccatctatcc
    49501 ctttatccaa ccaccatcgg atcatattga atagttcttc tttcaatttc tcgctttccc
    49561 aatttaagtc tggttgttct tttgcaaaaa catgcagata atactgtcca gtttcatgag
    49621 aatactccca agcagaaccg ccgaaaatcg atgtccaatt attcggttct ttgccatcta
    49681 ccccgtcgac ccaaatgtaa taatcgcgat acgggctttc tttagatttt ttcgattctt
    49741 caaaccattg atgctgatcc gaactatggt tgatgacaag atccataatc actttgatcc
    49801 ctaattcatg tgcttctttt agcaataaat caaaatcttc catcgttccg aatctctctt
    49861 ctatatcttg atagtcactt acgtcataac cattatcgac aaatggagat tgatagatcg
    49921 gattgatcca gataaagccg ataccaagat ctttcaaata attaagtttt tcacggatcc
    49981 cattgatgtc tccttgacca tcgccattgc tatctttgaa actagcagga tagatttgat
    50041 agcctactgc ttctttccac catttttgtt cttgtatcat atcgtttcac tcctttaacg
    50101 gtccctcttg attataaccg attctatttt agaagtatcc ttgttaccgg taactagaaa
    50161 cgaaaagctc aacgaatctg ctaagtgcgt tcgttgagct ttattttatc atagatagcg
    50221 ggcaatcagt tgaatcattt gcttgggaaa ttcattcaag tcagtaatat ctacaaaacg
    50281 actttcaccg tagatcgact tgatttcttc tttgtcttgg ccaatagctg cggagacaaa
    50341 taggattcct tgacgttcat attctttcaa aacagactgg atatcttctt ttgctttttt
    50401 gcctgtataa tccggcaatg cttttggctg accgtcacta atattaagca acagtttggt
    50461 tgttgcggat tgtctgttta atttttctgc tgctaaacga agtgcagcac catcacgatt
    50521 gttttgcctt ggtttcatag tgactaattt ttcatttaac cattgaaatc cgtcttcaaa
    50581 ttctttataa gaaaacaaag aagtcttttc tcttgacgaa cggtctgctg tatctccgta
    50641 aatcaacaat gggatatcga ctttcttaga aaattcggca atggcaatcg ctgctttttt
    50701 tgccgcttcg atccgatcat cccgtatcat tgacccagat tcatcgatcc gtacagctac
    50761 cgctaaagaa ggttgctcat gtggcgggtt ctttttgtca aaatttctca aatcaccata
    50821 tgctactttg cttgcattga accgctgacc ttcgtatttt cctttttggt acgtctctgt
    50881 ttgttcatta tccagtaact ctaaaatttg ccggctaagt gattctacta ttggcattac
    50941 ttgcatgtgt agctcacgtg cttccggcgt ttttccttgg aaagctggtc ggtgaacgat
    51001 gagaccttct ttttcatgaa tcgtttcctt catgatttgt cgcgcttctt tattcagttg
    51061 cttcttgatt tccttatcat gtgcttccat gtcagaagca gacatttctt gagaatcatc
    51121 tcctgtttgt tgttcttttt gattttttcc ttcgatatcg ctactgcgtt ctttttgcat
    51181 ttgaggagga atctcgtttt tttctattgt ttcttcttgt ttctcttctc cctcatttgg
    51241 ttcttcagca gacaacgatt tcttttcatc taagcgcaga atttcttcag cattttcagt
    51301 gatgtttcgt tcatttgctt cttcggcgct aatggttctt tcttttgtat gctcagattg
    51361 aggatgaccc catttttctt ttctcaatat gtcctgaagt tccagtagca atccactgtc
    51421 agacaaagct tgttcaagaa ttgccaattc atctgaatca gtggataatt tatacaaaac
    51481 ttttggataa atcgactgga gtaaatcttg tccttgctta taggtatttg cccaataaaa
    51541 ataagaacgc attcctgcta cacctttgat ggcgttagct tttgcagttt catccaacaa
    51601 acggataatc tctgccatct ttaagagaag ttcttgtttt tggatcatag ttttcgccat
    51661 cgcacgttct gccatgactt ctaaggcagg aagatccatt ttttccgcat gttgcatccg
    51721 atctcttaaa gattcattta atgggcgatt tccttggtaa tttcgatttg tagtgatcac
    51781 aatgacgcaa tctggatggc gacgaatgat tcctgtcggt aaattcaaca tcccatttgg
    51841 ttcgagcgct gagttaagtg ccacaagaac agaggcatct cggatgacag tcggctcttg
    51901 aatctctaat aaatagcctt tttcaatcgc tcgaacgatt tcagaagggt aatagctgta
    51961 atggattgca tctggataat cagcttcgat cctttgaact agctctgcta atttttcttt
    52021 agcagaaaaa ggatcaatgc cgtaatggtt ctggatcagt gtcagtacag cttcaagtgt
    52081 atctgtttgg taaatagctt ctagtaattc ttggtcttgt tccttattag aatctactac
    52141 aggtaataag gctccgaaaa catctgattt gtccatatct gcaaaacagg tcacttttgt
    52201 atagggaagt tgcagatcag cagatagtgc tttggctaat tgtgtcttcc ccgacccggc
    52261 atctccttcc aaaagtacgt tactgatttt catttcagga tcttgccaat tccctttgat
    52321 ttcggaggcg atccgccatt cttctgcact tgtccgatgg cttttcggtt tcttccagat
    52381 catttgcttt tctagccgtg aaaaggttcg gttttctgtt agtgagaaat tctccatgct
    52441 tctcttcccc ttttgatcat ctctgtctat taatatccta gctcatctaa aagacgtttt
    52501 aaaatacgga atcgttcccc aatcagttct ccgtcttttt gtagtgtatc gtaatacaaa
    52561 atgatgtctt catcgatctt tggatttttg gtatctactt ctaccgtcat tcggttccct
    52621 tgtaatccaa tgatgtctac gactggattt tctggatcat aaaatttttc ataacgataa
    52681 gcatcttgga agtataaact tccttgcgca gctaagatcg ctaaatctaa aatacgtgtg
    52741 attggtaatt cttccgactg tcgtgaccat ttttctccag tatgtcgcca taccttgcct
    52801 gagatatcga cttttccacg atcattccac tgagcaaggc ccagtgaaag cccttttgca
    52861 tccatattta atgcatttct tccatcaact ttatcatagt cttctacaac gattactggt
    52921 ttatgtttta aatttgttgg tatttccatt attttaactc ctcatccttt tatttttagt
    52981 aaataagtaa attagtagtt tactcattta ctaaactata tgcgacatta tagaggattg
    53041 aagtgaggat tgcaaagaaa aagagcttca ttgatagaaa taaaaacacc atctcgttct
    53101 tttacacgct cattagtaac atgtagaaag tccaagatga tgttggaat
//
LOCUS       DACECY010000015        49161 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.016, whole genome shotgun sequence.
ACCESSION   DACECY010000015 DACECY010000000
VERSION     DACECY010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.016"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            212..604
                     /locus_tag="IWO38_000902"
     CDS             212..604
                     /locus_tag="IWO38_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0313009.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="IWO38_000903"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="IWO38_000903"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ0313010.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="IWO38_000904"
     CDS             3165..4775
                     /locus_tag="IWO38_000904"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ0313011.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="IWO38_000905"
     CDS             5067..5936
                     /locus_tag="IWO38_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ0313012.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="IWO38_000906"
     CDS             6101..7372
                     /locus_tag="IWO38_000906"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ0313013.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /locus_tag="IWO38_000907"
     CDS             7369..8664
                     /locus_tag="IWO38_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ0313014.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="IWO38_000908"
     CDS             8763..9026
                     /locus_tag="IWO38_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ0313015.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="IWO38_000909"
     CDS             9471..9920
                     /locus_tag="IWO38_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0313016.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="IWO38_000910"
     CDS             9977..11395
                     /locus_tag="IWO38_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0313017.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSISTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="IWO38_000911"
     CDS             11395..12255
                     /locus_tag="IWO38_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ0313018.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="IWO38_000912"
     CDS             12429..13202
                     /locus_tag="IWO38_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0313019.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="IWO38_000913"
     CDS             13349..13714
                     /locus_tag="IWO38_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ0313020.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="IWO38_000914"
     CDS             complement(13862..15070)
                     /locus_tag="IWO38_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0313021.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="IWO38_000915"
     CDS             complement(15089..15367)
                     /locus_tag="IWO38_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313022.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="IWO38_000916"
     CDS             15560..16216
                     /locus_tag="IWO38_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0313023.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="IWO38_000917"
     CDS             complement(16658..17455)
                     /locus_tag="IWO38_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ0313024.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="IWO38_000918"
     CDS             complement(17452..18636)
                     /locus_tag="IWO38_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ0313025.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="IWO38_000919"
     CDS             complement(18661..19908)
                     /locus_tag="IWO38_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ0313026.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="IWO38_000920"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="IWO38_000920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ0313027.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="IWO38_000921"
     CDS             21888..22202
                     /locus_tag="IWO38_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ0313028.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="IWO38_000922"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="IWO38_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ0313029.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="IWO38_000923"
     CDS             22887..23531
                     /locus_tag="IWO38_000923"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ0313030.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="IWO38_000924"
     CDS             23546..23875
                     /locus_tag="IWO38_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313031.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="IWO38_000925"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="IWO38_000925"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ0313032.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="IWO38_000926"
     CDS             24863..25687
                     /locus_tag="IWO38_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ0313033.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="IWO38_000927"
     CDS             25680..26027
                     /locus_tag="IWO38_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ0313034.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="IWO38_000928"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="IWO38_000928"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ0313035.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="IWO38_000929"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="IWO38_000929"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ0313036.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IWO38_000930"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IWO38_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ0313037.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IWO38_000931"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IWO38_000931"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ0313038.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="IWO38_000932"
     CDS             31684..32451
                     /locus_tag="IWO38_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0313039.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="IWO38_000933"
     CDS             32441..34483
                     /locus_tag="IWO38_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0313040.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="IWO38_000934"
     CDS             34764..34913
                     /locus_tag="IWO38_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ0313041.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="IWO38_000935"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="IWO38_000935"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ0313042.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="IWO38_000936"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="IWO38_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ0313043.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="IWO38_000937"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="IWO38_000937"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ0313044.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="IWO38_000938"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="IWO38_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ0313045.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="IWO38_000939"
     CDS             39330..40412
                     /locus_tag="IWO38_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ0313046.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="IWO38_000940"
     CDS             40412..41215
                     /locus_tag="IWO38_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ0313047.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="IWO38_000941"
     CDS             41285..42025
                     /locus_tag="IWO38_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0313048.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="IWO38_000942"
     CDS             42036..43040
                     /locus_tag="IWO38_000942"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ0313049.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44929)
                     /locus_tag="IWO38_000943"
     CDS             complement(43085..44929)
                     /locus_tag="IWO38_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313050.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            45141..45659
                     /locus_tag="IWO38_000944"
     CDS             45141..45659
                     /locus_tag="IWO38_000944"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0313051.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            45787..46470
                     /locus_tag="IWO38_000945"
     CDS             45787..46470
                     /locus_tag="IWO38_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ0313052.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            46779..46852
                     /locus_tag="IWO38_000946"
     tRNA            46779..46852
                     /locus_tag="IWO38_000946"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46813..46815,aa:Arg,seq:tct)
     gene            47011..47370
                     /locus_tag="IWO38_000947"
     CDS             47011..47370
                     /locus_tag="IWO38_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ0313053.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            47493..49124
                     /locus_tag="IWO38_000948"
     CDS             47493..49124
                     /locus_tag="IWO38_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ0313054.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
BASE COUNT        15807 a         8509 c        10224 g        14621 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgattt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt agtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcattcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    44941 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    45001 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    45061 tttttacagc tataatttga aaaaaattta tcgttattat atgattaaga aggattgaat
    45121 aaagaaagaa gttgaaaacg atacctgatt acaaaaaaat aacttttata agttctataa
    45181 cgttgattat atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt
    45241 tgactcctta tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga
    45301 tatatgtacg accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg
    45361 aaaattcagg aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag
    45421 atttttatac aaaagctgat acaaagactt atattgagcc tggagtagta aaaaagagaa
    45481 atattatcgg tagtcccatc tttcagattc catatattgg actagtcctt tcaaaaacgg
    45541 catttgcatg gattcagggg ttattcttta cgattgcttt ttgcctaaca ggaatcacag
    45601 catggaatac ctttgtagag ttgaaaaaga agcggagacg aacacagaaa tttttgtaag
    45661 aatgttaaaa acataaatgt attccagaga tccaacgact cttctgaaca tgaaacacga
    45721 gaaaacactc atgcagcttg tagatttagt cagttgttcg tataatgtgt gaagaggagg
    45781 agtagtatgc gcaatgaaat gatgcatcgt cctgtagtca aagaagaact attggatttt
    45841 atgcgtaccc agcaaaaaaa attaccgggt gagctaggca aactggaaga agaagctcat
    45901 gtagcagaag taccagttat tccccatgaa acagttgtat ttttgaaatt tttacttgga
    45961 cagcttcagc ctgaacgaat cttggagatc ggagcagcta tcggtttttc atcaagtgtc
    46021 atggccacga caatcggaga aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg
    46081 atcgagaaag ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt gactttgctt
    46141 gaaggtcaag cagcagatat cttgccggag ttgtctggtc cgtatgactt tatttttatg
    46201 gatagtgcaa aatcaaaata tattgaattt ttacctgaat gcttacgttt gttaagaaaa
    46261 ggtggcgttt tgatggtaga tgacatcttc caaggaggaa cgatcttgct cccagatgaa
    46321 gagattcccc gtggcaaacg agctatccat cgtaaattaa atgaatttct tcgtgttgtg
    46381 atggatcatc ccgatttgac atcgacaatc ttgccattag gtgatggtgt gatcttaatg
    46441 acaaaggaat cagaaacaat cgagttgtga tacatgatta tggcagcctg tagataatcg
    46501 attgaatcga gggctcaatt cgaacttgat gtaaaagttt ctatatttgt aagtcaatta
    46561 aaggatacag gaaatctaaa taaacaggag tagcatgaag tcaatgtagc tcctgttttc
    46621 aattgataac cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag
    46681 ttgggccaaa ataattcatt atttgctaat taaataacta tttcgtattg acagaagcaa
    46741 tttaattatc tataattatt gatgttccgc tgattcttgt catagtagct cagctggata
    46801 gagcatccgc cttctaagcg gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa
    46861 ccgttagaaa ctgaggtttc taacggtttt tctttgtttt ttgtgccaat attgtttgtt
    46921 ttcaaaggca aataaaataa aaaaacatcc ttatcgagta caattataag gagcaaaaaa
    46981 ataaaactca tcagaaaaga ggaagaagat atgacgatac tatctagaat tttagtgact
    47041 ctagttgcaa ttgaattttt ttatatcatg tatatcgaaa cggtgagaac tgattctgac
    47101 acgacaagta gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca
    47161 ttgtttaaga accaaggagt gtacaatgga ctattaggag tgggactact ttatggagct
    47221 tatctttctt ccgcatctaa agaaatcaca agtatgctat taatatctat tttttttgtt
    47281 gcattatatg gcagtttgac tagcgatcgt tggattttac tcaaacaagg tgggctagct
    47341 ctgttggcat tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag
    47401 gaaactttgt gaaaaatatt actttatctc atgaaatatg atattctaat gaatggtatt
    47461 ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt tcctcactaa
    47521 aaaaagaatt ttcaggttat gatctttcaa gatttcagaa agatcttttg gctgggatca
    47581 cagtagctgc tgtagcattg cctttagcct tggcttttgg ggtatcaagc ggagctgatg
    47641 cggccgcagg tttgattacg gctatcattg ccggtatcgt tattggcggt ttatcaggcg
    47701 gtttttatca aatatcaggt ccgacaggtg cgatggcagc tatcttgatg tctattgcgg
    47761 caacaaaggg aatgcaaggt gtattgcttg ctactttttt agcgggtcta tttttacttg
    47821 cagcaggact tttccgatta ggaacactga cttcttttat cccagcaccc gtaattacgg
    47881 ggtttacttc aggtattgct atcattattg cattaggtca gatcgataat ttatttggtg
    47941 ttcactcgga aggtacaaac gttattgaaa aactaagtag ttatcaaacg ataggatttc
    48001 cgataaacac agcctctttg atcatgggag ggctagtcat tttagggatg tttatctatc
    48061 caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc tatcattttg gcaacagcag
    48121 ttatgctatt gttccattta ccagtagcaa ctgtgggtaa gattccacaa acattgatca
    48181 gtagtaatcg tttgaatatg ggagatttta gcttttctgc cttgcaacaa gtggcagttc
    48241 cagctattag cattgcttta ttaggaatga ttgaaagctt gctatgtggt gcttcggctg
    48301 gccgaatggc aaataagccc ttagatagca atcaagaatt aatggcgcaa ggaattggaa
    48361 atctgttgct tcctttcttt ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg
    48421 tggccatcaa atctggtgca caaactagat tagctggtat gatacatgca gttgtcttac
    48481 tgctctccat gcttgttttt gcgccagtga tgtcacagat tccaatgcca gcacttgcag
    48541 gtgttttgat tgtgacggct tggcgtatga atgaatggga aacgatcaag gagctattta
    48601 ctaataaata ttggtcagca gttctattgt ttatccttac gatgatttgt acagttatct
    48661 ttgatttaag tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca
    48721 aaagcgctgc tatcacgatt tcattagaaa cgatcgattg gcaaagaatg gggctgccag
    48781 aaacggaaaa gctgaataac tggacagtag tgtatattag tggtccactg ttttttatgt
    48841 cttcagaaaa actcaaaaat aaattggaag aactaaatca tcacgatggt gtcattttct
    48901 ccatgcgagg cgtaccaagt attgatttga cggcacgtgc agtttttgat gaatttcaag
    48961 aaaaagcaga gcaaagagga caaacgatta tctatacttc cttgcagcca gaagtagaaa
    49021 aacaactttc tcacttgtgg gaacatacat tcacgcatca acatcaaaca gttgctcatg
    49081 cgttgacagc tttacatgcg aaatatactg caattagcca ttaaaacaat gaaaaagaac
    49141 agacaagact gtaaactgaa g
//
LOCUS       DACECY010000016        48456 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.017, whole genome shotgun sequence.
ACCESSION   DACECY010000016 DACECY010000000
VERSION     DACECY010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.017"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            150..635
                     /locus_tag="IWO38_000949"
     CDS             150..635
                     /locus_tag="IWO38_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ0313055.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            628..1311
                     /locus_tag="IWO38_000950"
     CDS             628..1311
                     /locus_tag="IWO38_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ0313056.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            1437..4082
                     /gene="polA"
                     /locus_tag="IWO38_000951"
     CDS             1437..4082
                     /gene="polA"
                     /locus_tag="IWO38_000951"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ0313057.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            4127..4963
                     /gene="mutM"
                     /locus_tag="IWO38_000952"
     CDS             4127..4963
                     /gene="mutM"
                     /locus_tag="IWO38_000952"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ0313058.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            4927..5556
                     /locus_tag="IWO38_000953"
     CDS             4927..5556
                     /locus_tag="IWO38_000953"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ0313059.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            5608..5940
                     /locus_tag="IWO38_000954"
     CDS             5608..5940
                     /locus_tag="IWO38_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ0313060.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            6252..6743
                     /gene="nrdR"
                     /locus_tag="IWO38_000955"
     CDS             6252..6743
                     /gene="nrdR"
                     /locus_tag="IWO38_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ0313061.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            6766..8139
                     /locus_tag="IWO38_000956"
     CDS             6766..8139
                     /locus_tag="IWO38_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ0313062.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            8139..9065
                     /gene="dnaI"
                     /locus_tag="IWO38_000957"
     CDS             8139..9065
                     /gene="dnaI"
                     /locus_tag="IWO38_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ0313063.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            9194..9634
                     /locus_tag="IWO38_000958"
     CDS             9194..9634
                     /locus_tag="IWO38_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ0313064.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            9778..10479
                     /locus_tag="IWO38_000959"
     CDS             9778..10479
                     /locus_tag="IWO38_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ0313065.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            10476..11597
                     /locus_tag="IWO38_000960"
     CDS             10476..11597
                     /locus_tag="IWO38_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ0313066.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            11612..12844
                     /gene="pepT"
                     /locus_tag="IWO38_000961"
     CDS             11612..12844
                     /gene="pepT"
                     /locus_tag="IWO38_000961"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ0313067.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            12976..13884
                     /locus_tag="IWO38_000962"
     CDS             12976..13884
                     /locus_tag="IWO38_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ0313068.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            13919..14191
                     /locus_tag="IWO38_000963"
     CDS             13919..14191
                     /locus_tag="IWO38_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ0313069.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            14202..15248
                     /locus_tag="IWO38_000964"
     CDS             14202..15248
                     /locus_tag="IWO38_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ0313070.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            15499..18108
                     /gene="clpB"
                     /locus_tag="IWO38_000965"
     CDS             15499..18108
                     /gene="clpB"
                     /locus_tag="IWO38_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ0313071.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(18259..18930)
                     /locus_tag="IWO38_000966"
     CDS             complement(18259..18930)
                     /locus_tag="IWO38_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313072.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(18923..19417)
                     /locus_tag="IWO38_000967"
     CDS             complement(18923..19417)
                     /locus_tag="IWO38_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ0313073.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(19437..20657)
                     /locus_tag="IWO38_000968"
     CDS             complement(19437..20657)
                     /locus_tag="IWO38_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0313074.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            20804..22639
                     /gene="lepA"
                     /locus_tag="IWO38_000969"
     CDS             20804..22639
                     /gene="lepA"
                     /locus_tag="IWO38_000969"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ0313075.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            22835..23746
                     /locus_tag="IWO38_000970"
     CDS             22835..23746
                     /locus_tag="IWO38_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0313076.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            24742..24993
                     /locus_tag="IWO38_000971"
     CDS             24742..24993
                     /locus_tag="IWO38_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313077.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            25076..25348
                     /locus_tag="IWO38_000972"
     CDS             25076..25348
                     /locus_tag="IWO38_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313078.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            25438..25677
                     /locus_tag="IWO38_000973"
     CDS             25438..25677
                     /locus_tag="IWO38_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313079.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            25824..30107
                     /locus_tag="IWO38_000974"
     CDS             25824..30107
                     /locus_tag="IWO38_000974"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAQ0313080.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            30206..30571
                     /locus_tag="IWO38_000975"
     CDS             30206..30571
                     /locus_tag="IWO38_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313081.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            30573..31025
                     /locus_tag="IWO38_000976"
     CDS             30573..31025
                     /locus_tag="IWO38_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313082.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            31639..32949
                     /locus_tag="IWO38_000977"
     CDS             31639..32949
                     /locus_tag="IWO38_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ0313083.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLIKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            32972..33283
                     /locus_tag="IWO38_000978"
     CDS             32972..33283
                     /locus_tag="IWO38_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313084.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            33644..34900
                     /locus_tag="IWO38_000979"
     CDS             33644..34900
                     /locus_tag="IWO38_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0313085.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            34897..35442
                     /locus_tag="IWO38_000980"
     CDS             34897..35442
                     /locus_tag="IWO38_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313086.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            35443..35943
                     /locus_tag="IWO38_000981"
     CDS             35443..35943
                     /locus_tag="IWO38_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313087.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            36024..36580
                     /locus_tag="IWO38_000982"
                     /pseudo
     CDS             36024..36580
                     /locus_tag="IWO38_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            36600..36944
                     /locus_tag="IWO38_000983"
     CDS             36600..36944
                     /locus_tag="IWO38_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313088.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            36963..37874
                     /locus_tag="IWO38_000984"
     CDS             36963..37874
                     /locus_tag="IWO38_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0313089.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            37953..38945
                     /locus_tag="IWO38_000985"
     CDS             37953..38945
                     /locus_tag="IWO38_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0313090.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            38942..39160
                     /locus_tag="IWO38_000986"
     CDS             38942..39160
                     /locus_tag="IWO38_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313091.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            39173..39583
                     /locus_tag="IWO38_000987"
     CDS             39173..39583
                     /locus_tag="IWO38_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313092.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            39635..42163
                     /locus_tag="IWO38_000988"
     CDS             39635..42163
                     /locus_tag="IWO38_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ0313093.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            42179..44389
                     /locus_tag="IWO38_000989"
     CDS             42179..44389
                     /locus_tag="IWO38_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313094.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            44397..45488
                     /locus_tag="IWO38_000990"
     CDS             44397..45488
                     /locus_tag="IWO38_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ0313095.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            45509..46051
                     /locus_tag="IWO38_000991"
     CDS             45509..46051
                     /locus_tag="IWO38_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313096.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            46273..46503
                     /locus_tag="IWO38_000992"
     CDS             46273..46503
                     /locus_tag="IWO38_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313097.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKGYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            46517..46849
                     /locus_tag="IWO38_000993"
     CDS             46517..46849
                     /locus_tag="IWO38_000993"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313098.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            46894..47442
                     /locus_tag="IWO38_000994"
     CDS             46894..47442
                     /locus_tag="IWO38_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0313099.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            47449..47901
                     /locus_tag="IWO38_000995"
     CDS             47449..47901
                     /locus_tag="IWO38_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313100.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            47998..48333
                     /locus_tag="IWO38_000996"
                     /pseudo
     CDS             47998..48333
                     /locus_tag="IWO38_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        16510 a         7756 c        10361 g        13829 t
ORIGIN      
        1 ttggctgttc ttattatttg atgcaggaca gctttttcac gacctctgta aactatagga
       61 atctgttaaa tcaagggagc agaacttgcc ttttacgcta cttttggtac aataatgtat
      121 gattaaaaat aaactggagg gtttctatct tgaacatagg taagccgaga aaactaggaa
      181 aaacaataaa tgaaatcgag gaaggcgact cgttatcctt aacagaatct attgaagata
      241 accagctttt gctgtatttg ggattgacga atgatgcaaa tcctttgtat atccaacatg
      301 actttgcaca aaaaacagaa tatcaacagc caatcgttcc atcagtgatg ctgatgggga
      361 tcatcacaag tgcaatttca aagcatcttc ctggaccagg atcacatgtt gtcaattttt
      421 ctgtcaattt tattgaaccg gttttccatt atgaaacact gacttttcag tttgaggtca
      481 taaaagtaga taagatgaag gatgtggtga cgatttctgt agaagcggag aatacgaacg
      541 aagatcgtgt gttggatgct gtcgttatgg tccaaccgcc acaagttcct gaattggaaa
      601 tagcaatcga aagtgaaggt gaattgaatg aataatccaa gtacatcaat gtcaggtatc
      661 aatcgttttt atgcaaaagt ttatggattt ttaggtatgg gacttgccct tagtgcagtc
      721 acttcttact tggctctttg ggtatttcct ttgcaattag caacttttat caataatttt
      781 ccactaggct ttgtgggtct gtggatcgtt gaactgattt tagtaatggt tctaggcgta
      841 aaagcacaaa agaatccgag cttagcaatc ggtggattta ttgcctactc tcttttgaat
      901 ggcataacat tagcggttac tcttgcatac tacgatattg gaacagtaac gcaagctttc
      961 ttgacagcta ctttgatgtt catggggatg tctgttgtcg gagtaacaac aaaaagagat
     1021 cttagcgggg ttggaagagc aggacaggca gctttaatcg gagtgatcat tgcactagtc
     1081 ttgaacatct tcattttgag aagtagcgta gttgagctgt tcatctccct actgttagtc
     1141 gtcatctttg cagggattac agcttatgat aaccaaagca tccgtaatat ctatgtccag
     1201 acaaacggaa cagcacatag tggaattgct gtattcatgg cattgaagct gtatttggac
     1261 tttatcaact tgttcttggc gtttctgaga atctttggac ggaacaacta aaaaagaaaa
     1321 aaattcatca gttggggcta taccgaatta tttggtatgg tcccgctttt tcttttcgca
     1381 ttccgcagga ttctttgtta gaatgaataa gaactttaca gtaaggagtt aagaagatga
     1441 caaaaaataa attattactc gttgatggga acagtgtggc ttttcgcgca ttctttgcgc
     1501 ttcacaattc tcttgaacgt ttcaaaaata gaaacggtct tcatacaaat gcaatctatg
     1561 cattcaatac gatgtttgaa aatgttatgc aaaaagagca accgactcat gttctggttg
     1621 cgttcgatgc aggaaaaacg acttttcgga cagaaatgta tgcggagtac aaaggtggaa
     1681 gatccaaaac acctggcgaa ttcaaagaac aaatgccata tatacgtgat ttgttgacag
     1741 gactaggggt acagtattac gaattgccca attatgaagc agatgatatt atcggtactt
     1801 tggcagaaaa agtagacaaa gatcaatttg atgtcgtcgt gctttcaggt gatcgggatt
     1861 tgactcaatt agctagtaaa gaagtaaaag tggatatcac tgtaaaaggt gtcagcgata
     1921 tcgaaagcta tacaccagaa catgtagcgg aaaaatatga tgggttgacg ccaaaacaga
     1981 ttattgacat gaagggacta gccggagatg cctctgacaa tatccccggt gtgactaaaa
     2041 tcggtgaaaa aacagctatc aagctattga aacaatatgg ctcagtcgaa ggaatttatg
     2101 aacatatcga tgaaatgaag caaagcaaaa tgaaagaaaa tctgataaat gacaaagagc
     2161 aggctttctt atcgaaaaaa ttggcgacca tcaatacttc agcgcctgtg gatgtctcta
     2221 tcgactcatt gaagtatgaa gggaaaaatc tcgataaact cgttccgttt tataaagaaa
     2281 tggatttcaa tcagttcctt tctaaattga atatcgtaga agaagaagta aaaatggatg
     2341 atattttgtt tgaagtcgtt catgaattca aagaggaaat gtttactacg gatatggcgt
     2401 tgtacgtgga aatgatgggc gacaattatc atacagagga aatcgtcgga gtagcgtggg
     2461 ggacagaaaa gaaaatttat gtcacgaacg atctatctgt attcgaaaac cgagcgttcc
     2521 attcttggat cactgatccg actcggctga aaaaagtata tgatgcgaaa cgaacttatg
     2581 ttgctttaaa ccgctatact ggaaaaacga aaggaatcga ttttgatgtc ttgctaggtg
     2641 cataccttct cgatacgaat gaaaaaagca cagatatcga aggaatagct gctcattacg
     2701 gatacaacga tatccaatca gatgaagctg tatacggcaa aggggcgaaa aaaggtttac
     2761 cagaagaaga ggaactcttt tttgctcatt tagcgcgaaa agtagctgca atcaatgcgt
     2821 tgacaccaaa attgagccaa gaactggcag aaaaaaacca agaagatctt ttccggaaaa
     2881 tggaactgcc attgtctatc attttggcag agatggaaat aagcgggatc aaagtagatg
     2941 cgactcgctt acaagaaatg aagggcgagt tctctgctcg cttaagagag atcgaacaaa
     3001 aaatctacga agaagccggc gaagaattca atttgaattc accaaagcaa ttaggagtga
     3061 tcttatttga aaaaatggga ttacccgtta tcaaaaaaac aaaaactgga tattccacag
     3121 ccgtagatgt actggaacag cttcgtgaac aagcaccaat cgtagaggat atcctcacct
     3181 atcgtcaaat tgcaaaaatt caatctacct atgtcgaggg actgctgaaa gtgatccaat
     3241 cagatggaaa agtgcatacc cgttatgtcc agacattgac ccaaacagga cgattaagct
     3301 ctgttgatcc taatttgcag aacatcccga ttcgattaga tgaagggaga aagatacgtc
     3361 aggcatttgt tcctagagaa aaagactggc tgatttattc ttctgactat tcacaaatcg
     3421 agttacgcgt acttgcgcat atctcaaatg atgaacattt gaaagctgcc ttcctagaag
     3481 gtcaagatat ccatgccagc acagcgatgc gtgtatttgg aatcgaaaag gcagaggatg
     3541 tcacaccaaa tatgcgtagg caggcaaaag cagtgaattt tggtatcgtg tatggcatct
     3601 ctgattacgg actttcacaa aatctaggaa tcagccgaaa agcagcacag caatacatcg
     3661 atacgtactt tgaaaaatac ccaggtgtca aagaatatat ggaaagaatc gtacgcgaag
     3721 caaaagacca aggctacgtc gaaacgctgt atcaccgccg tcgatatctg ccagacatca
     3781 attcaagaaa ttacaatatc cgttcttttg cggaacgaac agctatcaat acaccaatcc
     3841 aaggaagtgc tgctgatatt ttgaaaatcg cgatgatcga attggataaa cgattaaaag
     3901 aaacaggtct tcaagctacg atgctgttac aagttcatga tgaacttgtc ttcgaggtac
     3961 cggaaaaaga acttgaatca ttagataaat tggtgaagga agttatggaa caagctgttt
     4021 ctcttcatgt accactgatc actgacagca gttggggaaa aacgtggtac gaagcaaaat
     4081 aaacattgtt taagacagag caatataatg aaggtgaaaa gaaaaaatgc cagaattacc
     4141 agaagtagaa accgttcgta aaggattgga acggttagta gtaggaaaaa cgattcaaaa
     4201 agttcaggtc ttgtggccca agatcatcga gcaaccagag acacccatct ttgaggcttc
     4261 tcttgttggc gaaaccatcc aatccattgg acgtagaggg aagtttttaa tttttcatct
     4321 cgatcattgt gaactgatct cacacttgcg aatggaagga aaatatcaat ttacgaaaga
     4381 aaatacacca atcgataaac atacgcatgt acttttcttt tttgaggatg gaagccaact
     4441 tcgctacaac gatgtacgaa aatttggtcg tatgacaatc gtcgaaaaag gagcaagtgc
     4501 aacttaccga ggaatcatga aattgggacc tgagcctttg ccagatagct tcttattagc
     4561 agatttcgca aatggactga aaaagtccca caaagcaatc aagccattgc tgctagacca
     4621 acgtctagtg actggtctag gtaatattta tgtagatgaa gcattatggg aagcgaagat
     4681 ccatccagaa caaccggcga acacactgcg ccccaaagag gtagaacaat tacgtctatc
     4741 gatcattgat gttttggatc gtgcaataga agccggaggt accacgattc gcagttattt
     4801 gaatgcatta ggagaatcag gcggatttca agtagcactg catgtctatc agcaaactgg
     4861 caaaccatgt atacgttgtg gaacgccgat cgtgaaaaca aaagtggcac aaagggggac
     4921 gcactattgt ccgaattgtc aaaggctaaa aatcgcaaag taggatttgt tttaggtttg
     4981 acgggtggaa tagctaccgg taaaagtacg gcagccaaag tctttcagtc tcatggattt
     5041 cctttgatcg atggcgatgt gattgccaga gaaactgttg agcctggaac gcttgctctg
     5101 aggaaaattg tatcagtgtt tggccaagag gtattgcagc ctgatgggca actagatcga
     5161 gggaaacttg ggatgattgt ttttccatca aaggaaaaac ggcaaaaact tgatcagcta
     5221 ttagatccgt ttatccgaaa agcgataaaa gatcaaatcg cctctctaag ttccaatcat
     5281 cctttagtaa ttgtagatat cccattgctc tacgaaggtc attatgacca ctatatggat
     5341 gcagtagctg tcgtttatac tacgccagaa actcagcttc agcgtttgat gaaacgtaat
     5401 caactgacgc tagaacaagc gcagcagaga attagtagtc aacttccaat cgaagaaaaa
     5461 aagcagcgtg cggatatttt gttcgacaac aatggaacaa aagaaaaact tgtgggacaa
     5521 atcgaaaatt ggctttttgc taatcgcttt ctttgagtat ctgtgtcaat tttgctatcc
     5581 tgagtatata gaaagtgagg gatagcgatg ggatcattaa aaaatcaaaa ggataaagtc
     5641 gtaggtaacg caaaagaaaa aatcggcgag atgctcaata aagaaaatct aatcgatgaa
     5701 ggtcgtacgc aatcagaaac agcagaagaa aaagaaaagc agtaccaagc aagaatcgat
     5761 gaaatagaag accagcaggc agaagaagaa gcgaagcggc aagatttgac tcaagaacga
     5821 aaaaatccga cacctgatcg tagtaaaaca ccgctcccag aaagtgacca cattcccgat
     5881 gagcaaaaaa tcgaggaaca gcgaatgaaa cactatacag gaatcgaaga gaagttataa
     5941 aatagtaaaa aatcgagatg tgacttgaag cagatgagaa ggtgttcatc tgcttcaagt
     6001 tttttgtgct agatgactaa atcagctttt gtgtaagtta tttctcagta atcaaggtga
     6061 tcagtattgg aaaattgaaa tttagtaagg acaggggaca caattacgca tgaaagtaga
     6121 aaaaattgtt cttatatccg tgttttttgg gtaggacagt cgcgtttgtg atttgtttcg
     6181 tgttataata caactagttg taaagaaaga cacatccttt atataaaatg cgatgcaaga
     6241 ggtgagacaa aatgagatgt cccagatgtc aacataataa ttcacgagta atcgacagcc
     6301 gtcaagcaga tgatggacgt gcgatccgtc gccgtcgcga atgtgagaat tgttcgttcc
     6361 gattcactac atttgaacga attgaagctg ctccgttatt agtaatcaag aaaaacgggg
     6421 aacgcgaaga gttcaatcgc gataaaattt taagaggact catccgttca gcagaaaaaa
     6481 gacctgtagc tatggaacaa atggaacaaa tcgtcaacca tgtcgaaaac cgcgtacgca
     6541 gtctcggaga aaacgaagta tcgacgaatc taattggtga atatgtgatg gaagatttag
     6601 ttgatttaga cgaaatcgct tatattcgtt ttgcaagtgt ttatcgccag ttcaaagata
     6661 tgtctgtttt cttaaaagag ttgcaagata tcgtggataa agcaaaagaa aataataaag
     6721 agaataataa agaaaacaat taagaaacaa aaaggaggca tttccttgga aaatgcttgg
     6781 aaagagcttc aaccaaaaaa tatttaccaa ggaagaaaag cggctccgct gagagaagac
     6841 agcaagaacg ccttgctgta tctttatcag ccactaatcg gcggagaagc cttgagtatc
     6901 tatttcaccc tgttatcaga aatctctcaa gaatctggca aaggtcctga agggatgcat
     6961 gcagatctgc ttagtagtct aggctgcggt atcccgcaat tttatgatgc aagaaaaaag
     7021 ctagaaggaa tcggcttact ggatgtttat tttaaagaag atagtgatct gggacctcgt
     7081 tttatttatg agctgttaga accactcatt cctaaagcat ttttcaaaga ctctgttctg
     7141 gcttttcttc ttctggaaaa agttggtgaa cgacgattcc agaagcttac ccaattcttt
     7201 gaacctaaaa ggcatacagc agagggatat caaaaaatca caaaaaaatt tttagatgtt
     7261 tatcgcttca atgaagcagt gtacgctgct aatcaagaaa acatcgaatc gctgaataaa
     7321 tcttttgaag aatctgaatc agtagcatta cttgaagcag attcgacatt cgattggcag
     7381 tttttgaatg attggctggc aaaaaaacat attgcaagtc ttgatgatcc aatcatcaaa
     7441 caagtcgaca tgtatcatca gctttacggt ttcgatgaat tgacacttgg cgaactgatc
     7501 agtcaatctt atgactttag tgaacaaaaa gtatctgaaa aagaactgca aaaagccatt
     7561 ttggcaatgc agagacgtcc ggtgaagcaa acaaaagaaa cagttgcggt ttctgaaaac
     7621 agtaatgaac cctcgtcaaa cgaacatttg acaaacgcag cgcttcagct gataaaagaa
     7681 gcacagagca ttccaccgat gaaatatctg gaagcaatca agtcagaaaa aggcggatat
     7741 gtttctaaac aagaaaactg gttactgcaa gatcttgttt ctcaatcagg tctttccagt
     7801 agcgtgatca atgttttgct gaattatgta ctagtgatca agaataatgc tagcttaaat
     7861 gccagttatg taaatacagt ggcaaatgaa tgggcacaaa agaaaatcgc cacagcagaa
     7921 gaagcgatcc agcacattcg gcaaataagt aatcaagcaa aacaatcaaa acaaaagaag
     7981 caaacaaatt atcaaactgg caagcgaaat gttcgacggg aaaagcttcc tgattgggtc
     8041 aaccagccaa aagatgaaaa acagatcagc agcgagaaaa aagcagagat tgaccgccgt
     8101 tttaaagaat acctggcaaa aaaggagggt gatatctagt ggaagacgta ggcaaagaat
     8161 tgaagaggat catcagcaaa cgcaattatc aagaaaagta tgctgaaatg atcgctgaag
     8221 tcttaaatga tgcggatgtt caagcttttc taagcgcaca ccaagaagaa ttgacacaag
     8281 cggatattga aaaaagttac gctaaattgt atgaattcgt gcaagaaaag cgtaaatatc
     8341 aaaaaaacga tccaacgatg atcgctcccg gctatgagcc gaagctgacg ttgaattttc
     8401 attatgtgga tgtcacttat gtaccaacgg aagcattgtt agcacggcaa agacaagaag
     8461 aaatcagaag ccgagtaaaa gcaatggata tgccaaaaga tattcaatcg gcttcttttg
     8521 aaaactttga acgtacagat ggacgaggat atgcgagctt agaagcccta gattttgtag
     8581 agaaatatac tgctgatcca aaagggtatc ataaaggact gtatctagta ggaagttttg
     8641 gtattggaaa aacatattta ttaggagcaa cagcgagaga attagcgatt gctggattca
     8701 ccacgacgct agtccatttt ccgacatttg ccgttgagat gaaacaggca atcggaaaag
     8761 atcaagtggc agaaaagtta gatgcagtga aacgttcacc tattttgatg atcgatgaca
     8821 ttggtgcaga tgcgatgagt agttggatac gagacgaagt attcggtgtt atccttcaat
     8881 atcggatgca ggaacagtta ccaaccttct tctcatcgaa ttttacaatg gatgaattgg
     8941 aacagcattt gtccgtcact caacgtggag atgaagagcc gctaaaagcg aaacggataa
     9001 tggaacgcat acgttatttg acaaaagaaa tcgaaatgac tggtcgcaac cgacgcaatg
     9061 gttgaacagt tgacaagtcc aactgacatt tgacctagta gtaaaagcag gtcagcgaat
     9121 cattatatgg acagaaatga ccggagacaa tcaccgcaac aagtaatgca gaaacttaaa
     9181 cggaggattt tttatgaggg ctattttttc tagtatatct gtagttggtc tattttttct
     9241 tttagcaggc tgcggtagcg cagatagcaa cacaggttct gttgattcga ttgatgcggg
     9301 tcaaacagta aaaacaagca gtacgaaaac ggagagacaa agcatatttg tcggaacgat
     9361 tgaaagtatc actcaagtag aagcagaaga agaaacgctt cagttgaaac tcgtcgatgt
     9421 catggccaaa gaagatccag cgaatatggg aacgtcattc agcaatgacg gagtgatctt
     9481 aaatgctact gctgaaaaaa taagcgtgga cataaatgaa ttgaaaatcg gtgacaaagt
     9541 ccaatttaca ttagttgaga tgccgatcat gacgatgtct atcccaccgc aagttcctgg
     9601 agattcgatt attgatgttt cattgaacaa ataaccaata acattttttc aaaaataaga
     9661 aaatgttgtt ttatagaacg aaaaggtata ttttttaagg agatatggga atttttttat
     9721 tcctagtctc cttattttat ttggaaaatg ctataattat ctagaaggag tgaagctatg
     9781 aaccatttag atttatctca gcgcttagcc gcagtgggtg actttgtccc tgaatccgct
     9841 cgtttagcag acattggatc agatcacgct tacttacccg ttgcattgat attaagagga
     9901 aaaatcgaat atgcagttgc tggtgaagtc gttcaaggtc cttatgaatc agcacgtaag
     9961 caagtcaaaa aagatggatt agaagaccgt atcaccgtac gccttgcaaa tggtctagat
    10021 gctatccagc cacaagatgg tattacagct gtcacgattg cgggtatggg aggttcactg
    10081 atccgagata tcctagaagc tggcaagttg aatcaacgct taagcggaca agaacgtttg
    10141 atccttcagc caaacatcgg agagcgtaca ttacgatctt ggctgcaaga aaatcagtat
    10201 aaaatccaat cagaaaaaat acttgaagaa aataaaaaaa tatatgaaat cattgtagcc
    10261 gaaaaagaac aaaaaccagt tacttacaat gaaatggagc tgatgttcgg gccatttctg
    10321 cttgaagaga aaagtgagat cttcaaatca aaatggacaa gagagcttca acaaagaaaa
    10381 gctatccgtg aacagctcaa acaagcagct aatcaagagg cacgtcaaaa acaaatcgag
    10441 caggaaatcc aaatgattga ggaggtttta aagccatgac aatcagcgga cgcactttca
    10501 tccaaaagtt tgaaagctat tgtcctcagt ggctagctga agaaggagac ccagtcggac
    10561 tgcatatcgg aacattggat aagccaatcc aacgagtaat gatgacatta gacgttcgtc
    10621 cagaagtggt cgaagaagca atcaagaaaa aaatcgattt gctgattgct aaacatcctc
    10681 caatctttcg tcctgtaaaa cggctggtta ctgatcagcc acaagaaaaa atgtatgcag
    10741 atttgttgaa acatgatatt gccgtctatg cagcacacac gaacatggat attatttggg
    10801 atggactgaa tgactggttt tgtgaacttc tcggtattga agtggagagt tatttagtaa
    10861 agactcatga gattcattat aaaaaactgg ccgtttatgt acctgtggat catgcacaga
    10921 aaatgcgtga agtattagct gcagctggtg ctggtacaca aggtgattat accggtacaa
    10981 gttttacctc gattggacac ggtcgattta ctccggaagc tggtgcacaa ccagcgattg
    11041 ggaaagtagg gaagacagaa caagttcaag aggcaaaagt tgaggttatt ttgccggaaa
    11101 ctatcgaaaa acaagtgatt caagccatgc ggtcagctca tccttatgag gaacctgctt
    11161 atgatctttt cgctatagat gaaccagtcg aaatgtttgg gttaggacga gtaggtgaat
    11221 tgccgcaaga aatttctata gaggcatttg tagaacaagt aaaagaagcg ttccaactag
    11281 atggactgag aatagtccag ccaaaaaatg caaaatcctc tgtgaagcgc attgcgattt
    11341 gcggtggaag cggtgaaaaa ttttatccgc aagcaatcgc ccaaagagca gatgtctata
    11401 tcacgggaga tatctattat catacggctc atgatatgca aagcgctggg ttgattgcaa
    11461 ttgatcccgg tcattatatt gaatcgttat gcaagcaacg atttatcgag aaatttgaat
    11521 cttggaaaca agaagaaaat tgggatatta actttttcgt ttctgaaaca aatacaaacc
    11581 catttcaatt taattaaaag gagagaatac tatgtacgaa aatcttttgc cacgtttttt
    11641 acgatatgtc aaaacagaaa cacgctccga tgcaacaagc aaaacgactc catcgactca
    11701 gacacaagtt gcttttgcgc aagaattgaa aaaagaatta gaagagttag gcttagctga
    11761 tgttttctat aatgaagata acggattcgt tatgggaaca ttaccaagca atgttgatca
    11821 tgaagtgcgt tccattggat ttatttccca catggataca gcagatttta atgcagtagg
    11881 agtcaatcca caaatcattg aaaactatga tggcgagtct acgatcgtac tagatgcgga
    11941 agaacgctat acattgaata caaaagattt tccaaatttg aaaaattatg aaggccacac
    12001 attgatcaca acagatggca gtacattact gggagcagat gacaaatcag ggattgcgga
    12061 aatcatgact gcaatggaga tcttgatcaa taatccttct atccctcacg gtaccatcaa
    12121 agtggctttt ggtcctgatg aagaaatcgg tgtcggagcg gataaattcg atgttgagca
    12181 attcgatgta gattttgctt atacagtcga tggtggtcct ttaggtgagt tgcagtatga
    12241 aacgtttagt gctgctcaag caaatattac gatccaaggg aaaaatgtac atccagggac
    12301 ggcaaaagat acaatgatca atgctctaca gttagcaatc gattttcata atcagcttcc
    12361 agccgatgaa gttccagaaa aaacggaagg atacgaaggt ttcttccatt tgatggcatt
    12421 ggatgggaat cctgaagaag cttctatgag ttatatcatt cgcgatcatg atcgtgaaaa
    12481 attcgaagca cgcaaagcac aaatcacaca tatccaagaa gagttgaatc agcgatttga
    12541 tcaagaacga atcaaagtag atatgtatga tcaatattac aacatgaaag aaatcattga
    12601 aaaagatatg agcatcgtgg aattagcgaa acaagcaatg atcgagcttg gtatctctcc
    12661 agtgatcgaa ccagttcgtg gaggaacaga tggttcaaaa atctcttaca tgggtattcc
    12721 gacgccaaat ctatttgccg gaggagaaaa tatgcacgga cgattcgaat ttgtttcttt
    12781 acaagtaatg gaaaaagcaa cagacgtgat tgtaaaaatt gcagaattga acacaaagag
    12841 ttaaaatcga aaaagaacag cttatgacaa ggcgttcgtc ccaatgatgg aatgtcatct
    12901 gtctaaggct aaagggcagg acaatggaat tggttgtccc gccctctttt ttgaagagta
    12961 aataggaggt aaagaatgga aaagctgcta gttttttata acaaaagttc agggaaagat
    13021 gagggtgaga aattagcacg ctggtttcaa gattatgtaa aagaaaagca gccagaatta
    13081 tatgtagaac tagctgaaac tggcccatcg attgatgatg cggaattagt caagaaagca
    13141 gaagccatag aagctgatac tttagtaatt ataggtggag acggcacgat tcatcatatc
    13201 gtccaagcgt tccaaaaaac attatctcaa taccatgtcg gattacttcc aggtggaact
    13261 gtcaacaacc ttgcacgtgt tcttgagatt cccctcaaaa aagaagaagc tgctgacgtg
    13321 atcctggaag gcaatgtacg gaaaatagac tatggtcaag tcaatgatga agtcattatc
    13381 tctacactga caatcggaat cttagcagat acagcagttt ggatcagtca agaagaaaaa
    13441 cagcgttacg gctcgtggat ctttatcaag aagtttttta agttattgct gaagaagaaa
    13501 aaatatcatt tggatttaga aacagacgag gagcattgga aaggaaaatc ccaactgttg
    13561 acagtcacga tgtctaactc cgtgggcggt tttacaaatt ttgacgactc agcgacccta
    13621 gatgatggaa aatttcatgt aacgatcgta ccttccttag atattttccg atttattttt
    13681 tatttgccta gaatcatgag aggcaaattc tatacgatcc ccggcattac ttatttcaca
    13741 gccaaacgca tgaaaatcca agcgcaggaa aaatctgtgg gcactcgaac ggatggagac
    13801 acgacagatg atcttccagt tgaattatct gttgtatcaa aaggattaac agtttattca
    13861 ccaaagaaag aagaaaaagc ttagaaatag tagttaacag aaagttggaa gaagagtaat
    13921 ggatgtattg ttacacaatc ctttgtggtt ttaccttttc attgtgaatt tttacttatt
    13981 ttgtctgatg gggtatgata aacatcaggc tagaactagc ggctggcgtg ttcctgagaa
    14041 taatttgata tttgtcagcc tgttaggcgg tggtttggga ggattgatct ctcgaaaagt
    14101 tttccatcat aaaactcgga aaaagaagtt tactatagga ttcgctgcag gtgtacttgt
    14161 tgatttgatt ctcatttatt ttttccatta aggagcagga gatgtcgtct aaaggaacga
    14221 aaactaaaat cattctgaat ctgcttttgc tgattgtgat ttttgccatt atttattatt
    14281 taatcaatca atcatttgca gatatctttg ccgaattgct gtctaccagt gttgttgttt
    14341 tggcagcggc tgttatatta gggacagttt accaaatcgc tgaagggcgc tcaatcaaag
    14401 aaattgctga acctttcgcg ccgaatttta caacgaaaga tgggttcttg acctctagct
    14461 atattgcgtt ttaccgaatc gtttcgtttg gtacaggaac gctgatctcg gagatttatt
    14521 tttataacaa aaaaggaatg aagctatccc agggagcagg ggtgacagca ctccatatga
    14581 ttatgtacaa actggcagtg atcacttatg cattagctgg cttggtcatc caattctctc
    14641 ttttttattc taatggtcca tcaatgatct ggtttatcat cgctggtatc cttttgacga
    14701 tcgtgattgt tgccttttta ttggcgatgt ccgtcagctt gaatgttcaa atattttttg
    14761 tcagcttatc gaataagtta tttaaaagtg agcgtttgcg gaaatgggtg gatacatgca
    14821 atcagcagat ctactcttta agggaaacgg tccaaaccat tatccaagac aggactgcgc
    14881 tattgcggat ttatctatgg aatatgttaa aacttgtatt ttggtatgta cttccttatt
    14941 tgattctgat tgaaaaccat cctaaaatag attttctact gaccatttcg tttatcagct
    15001 ttgcagtagt gctttcaggg gttattccta cgcctgctgg tattggttcc tttgaatttg
    15061 tctatttgtt attattcaga ccagttgttg gaacagttga cgcagtttct tcgcttttac
    15121 tgtatcgttt cagttccttt atcttgccat ttctctatgg atttgtgtat gtgattgctg
    15181 aacggcgaag agtgatcaaa gaagaaataa tagaagtaaa aaaagagaat aaagaaaatc
    15241 aagggtaggt cttttataag atctaccctt tttatcaaat caaagaaaaa cgttatggat
    15301 aagataattt tacgtttgtt tggctctttt tttatataca tacaatcaat tttgtttttt
    15361 taagatttat ataagtcgag aaaaaaagtc aaaaaactat gtgtatattt tgaccaaaat
    15421 tgaccaaagc gatataatag tttttgtaaa gatcaaagaa ataagtgatc ttacctttaa
    15481 ttaggagggt atacctgtat gaatattgag aaaatgacta cgacgcttca agaagcgatt
    15541 gctgaagctc aacagattgc ggtcactcga aaacatcaag atatcgacat tgcccatgta
    15601 tggaaaatct ttttacaacc taatcatttt ggtcgaaatt tctatacgga tgccggattg
    15661 gatgtcgaat cttttgaaca tgaaattgat cgtctactgg atgagtatcc agttgtttct
    15721 ggtggaaacg tccaatatgg acaaaacctc agccagaatt tctttcgatt attgaatgaa
    15781 gcagaccaaa tacgtgaaag cttcggcgac gagtttcttt caacggaagt cgttatttta
    15841 gcgttgatga aattaaaaaa ttatccgtta actgtttatt tgaataaaaa cggattatct
    15901 gaaaaagaat tgcgtaaaaa catcgaagag atgagaggag gagaaagagt gacctcacaa
    15961 aaccaagaag aacaatacaa agcattagaa aaatacggcg ttgatttagt acagcaagtc
    16021 aaaagcggaa aaatggatcc aatcatcggt cgtgatgaag aaatccgtga tgtcatccgt
    16081 attctttcac gaaaaacaaa aaataatcct gttctgatag gagaacccgg tgtagggaaa
    16141 acggcaatcg ttgaagggct tgctcaacgt atcgtacgca aagatgtgcc tgaaaattta
    16201 aaagataaaa cgattttttc attagatatg ggtgcattga tcgctggagc aaaattccgt
    16261 ggtgaatttg aagaaagact aaaagcagtc ttgaaagaag tcaaaaaaag tgatggacgt
    16321 atcctcttgt tcatcgatga aatccacaat atcgtagggg caggaaaaac agaaggaagt
    16381 atggatgccg gaaatctgtt gaaaccaatg cttgctcgcg gtgaattaca tttgatcggt
    16441 gccacaactt tagatgagta ccgtcaatat atggaaaaag acaaagcgtt ggaacgtcgc
    16501 tttcaaaagg tattagtcaa agaaccaaca gtggaagata cgatcagtat tctgcgtgga
    16561 ttaaaagaac gttttgagat ccaccatggt gttaacatcc atgacaacgc attagtagca
    16621 gcagctactt tatctgaccg ttatattact gatcggttct taccggacaa agcaatcgat
    16681 ttgatcgatg aagccagtgc aacgattcgg gtagaaatga actctatgcc aacggaatta
    16741 gatcaagtta ctagacgttt gatgcagttg gaaatcgaag aagctgcact gaaaaaagaa
    16801 tcagatgatg caagtaaaaa acgcttgaaa aatttgcaag aagaattagc tgaacttcgt
    16861 gaagaagcaa atgcgatgaa gatgcagtgg gaaacagaaa aagaagaagt caattctgtt
    16921 tcagcaaaac gtgctgaaat cgataaagca aaacacgaat tagaagatgc agaaaataac
    16981 tatgatttag aacgcgctgc tgtattgcgt catggtacga tacctcaatt agaaaaagaa
    17041 ttgaaggaat tagaagctaa agcaaaagac agcgagatta agatggttca agagtccgtt
    17101 accgaaaatg agattgctca agtcgttggc agattaacgg gtatccccgt aacgaaactt
    17161 gtagaaggtg aacgagaaaa actaattaaa ttgaatgaaa cacttcataa acgagtgatt
    17221 ggtcaagatg aagcagttga tgcggtcagt gacgcggtaa ttcgttcacg ggctggattg
    17281 caagacccaa atcgtcctct aggttctttc cttttcctag gaccaactgg tgtcggaaaa
    17341 accgaattag cgaaagcgtt agctgagaac ttgtttgact cagaagatca tatggtacgg
    17401 atcgatatga gtgaatatat ggaaaaacat gctgtgtctc gtctagtcgg agctcctcca
    17461 ggctatgtag gttacgaaga aggtggtcaa ttaacggaag ctgtcagacg aaatccttat
    17521 acaatcgtct tgttggatga aatcgaaaaa gcgcatcccg atgtcttcaa tatcttgtta
    17581 caagtcctag atgacggacg cttgactgat tcaaaagggc gagtagtaga tttcaaaaat
    17641 acggttctga tcatgaccag caatatcggt tcacagttac tgctggaagg cgtgactgct
    17701 gatggaacga ttccagaagc agtggctgaa caagtcaaca cgctattacg tggaaacttc
    17761 aaaccagaat tcttgaaccg aatcgacgac acgattttat ttacaccatt gagcctagac
    17821 aacgtgaaag gaatcgttga caaaatggtg gcgcaattgg cacaacgtct ggaacatcaa
    17881 gaaatcctgc tgacaatcag tgatgaagcc aaaacgtgga ttgctgaaaa tgcttatgaa
    17941 ccagcatatg gtgcaagacc attgaaacga tttatcacta aggaagtaga aacaccacta
    18001 gctaaagaaa tcgttgcagg acatgttatg cctaaatcaa aagtaacgat tactttactt
    18061 gatggacaac ttcatttcaa aacagaagag ctagaagaaa tcgtctaatc ttccattgag
    18121 actataaaag aaaaaagggg tctgacacaa ctagtgtcag acctcctttt ttcaaataaa
    18181 cagtgttcca gaagtagttt cttcgaaaaa taagccgaaa tttcttgaag ctgcccgctt
    18241 ctgtcacaac ctcttttatt aggtattttt cactttttct aatttataac tttcaaagta
    18301 acgctcatct tttttacttt gagaccatgc aaacaatccc attaaggcag cacctatcgt
    18361 atttgtcagc aagtcgccca tcgtatccat caatgctgct cgtccgacaa acagtgtgcc
    18421 atcagaagca gcaaaacgtt gaagattcat gcctaagaac tggtcgcata agaattccca
    18481 gaactcccaa aacacgccgc aaagtccagc aaacgcaaag ccgaataaga ggaacaacca
    18541 aggactggtt tttgatatct cagcatcttt catcaagtaa ccaatcaatc catagccaag
    18601 agctgtcagg accattggac tcaccgcatg taagattttg tcccaaaaag agatgatact
    18661 gatcaaatgc atccctgtgc ctaaaaagac agagatgaac aagaaaaacc agtaaaacaa
    18721 aacgattgct gctggtattt taagtttgaa aactttcaaa atgacttgtg gcatgaaaat
    18781 caatgcaatc cctaataaag attccacgat aaatgtgatt ccttgagaag tcgaatattt
    18841 ttgtgtgata aattcataaa aattatagca gacaacgaat acaccaaaag caagtaaagc
    18901 tatttgcacc tttttgattt ctttaatcac tgaaagtccc tccatctaaa aatcttttta
    18961 cttctgaaag caactcaatt tctgaagaaa ccacttttcc gttcaatttg atcaaaccta
    19021 ttgtatataa gttcaggtag tgaaattgat tttcagctgt ttcttgtagt gcagcgattt
    19081 ttttcgggtg ggttccgcct tgctgtcgag tatcagtata taagccgatc actgggatgc
    19141 ctttcgcata agccacaccg atttccgatg ctaccccggc atcgatcgtc agcccatcta
    19201 gtaaagcaat cattaaatca ctagccaaca ctttttctgt gtcagcaaga gcaatcattc
    19261 gactgtctgc ataagcagtt ttgtcattga ttgcttcatt ttcttgagga agatagatcg
    19321 aaagctcaga agagagctct ctgatttctt taactaactg ttggttatat aacaaatcac
    19381 ttttagcaaa cattggtgcg gcaaaataaa ttttcatcta aacatcatcc tttttatcaa
    19441 aaataatcgc taaagtaagc ttccatgttt ggaatgatac gctctagtga cttcagtgtt
    19501 tcttcatctg tttctaaccg ttcgattctt gccaaataag aagcacggcg ataattcatc
    19561 atcaagcaag tcaatgtttg gatatccagt cgaacggatt ttcctatagg ttcatttaag
    19621 acacggactt gtccctgttc atcccatgtt aaagcgaaga caccattatt ccaaggagcg
    19681 acaggatctt caatgatgaa atgaaaagca tcagctgttc caacaaacgg aaaccgttga
    19741 aggaattctt ttacatcaac gatccgagcc ataaaatatg gttcgatctg ctctttgatc
    19801 tgactatctt caatcaaaaa tgctaaaggc tcatttttat aaatatctcc atgtacccag
    19861 tagatcatgg aaaaatgtgc ggaaataaag ttccataagc catttcgagc ttcttgatca
    19921 agatagaaca tttcttttac gtgaaatatt tcttcagcta cccagtagaa cagaacacct
    19981 cttggttcat ggttgctatc gtagtagaca gcggctgtgc gctcatcttc attttcgaaa
    20041 cgccaatact cttcccaatg aaaatcactg cggaataaag caccatggtt ttgttgggca
    20101 aatttgtcat aaacggaaaa tacgtctgga tgatcaactg gtaatcgctc aaccatacca
    20161 cttactggga tttgtttagg tagctgtgta tctctgattt taaaggaaag tttatcagac
    20221 atgatttccc atcctttacg ccgatagtag gggatactgt aaggatacaa ataagagatc
    20281 cattgcttat tcttgcgcat attatccaat gcaacgataa ttagatcttg catcaatcca
    20341 tggttagcat attctggata agttccaact ccagtcacac cacccatttt ataacgtgca
    20401 ccgtgaatat tgacttcaca aggatagata gcgatttgag agatcagttt attttcgtgg
    20461 aaccatccga acactttcga tacttccaaa atcggttttt tggaacggat aaaagctcgt
    20521 ttattctcaa acccgctttc ctcgatgtct gcttcagtca cttggaacac ataggaaagc
    20581 aattcattga attgatcgat atgttcttga gtgaccggtt ttaattcaag attttctcta
    20641 aaatcttttt gatccatttt ttcatctcca tttattagct tgataacagt atagcaaaat
    20701 tctacaaaaa aaacttctat agctgactaa ttaaaaactc tttaaattac tgcttttcgt
    20761 tggtataatg gaaagtgctg attatatgaa aagaggagac gctatggata taaagaaaat
    20821 gaaagaacgc caagagcaga ttcgtaattt ttcgatcatc gctcatatcg accatgggaa
    20881 atctacattg gccgatcgta ttttagaaat gacgcacact gtgacgtctc gcgaaatgca
    20941 agatcagctg ttagactcga tggatttgga acgtgaacgt ggaatcacga ttaaattaaa
    21001 tgctgtagaa ctcaattaca cagcgaaaaa tggagaaaca tatatctttc acttgattga
    21061 caccccaggg cacgtcgact tcacctatga agtctctcga agccttgctg cctgtgaggg
    21121 tgccgtttta gtggtcgatg cagcacaagg aatcgaagca caaacattgg ccaatgttta
    21181 tttagcgtta gacaatgatt tggagatcct gccagtcatc aataaaatcg acctacccgc
    21241 agcagatccc gaacgtgtcc gtcaagaaat cgaagacgta atcggaatcg atgctagtga
    21301 agctgtatta gccagtgcca aatcagggat tgggattgaa gatattctcg agcagatcgt
    21361 ggaatatgtt cctgcaccaa gtggagactt agatgcaccg ctaaaagctt tgatctttga
    21421 ctctatttat gatagttacc gcggtgttgt tctaaatgtc cggatcacgg acggtatggt
    21481 caaacccgga gacaaaatca aactgatgag taatggcaaa acatttgatg ttacagaggt
    21541 cggggtcttc tcgccaaaag cagttgctag agactttttg atggtcggtg atgtcggtta
    21601 tatcacagca agtatcaaaa ctgtccaaga tacacgagta ggggatacag taacgttagc
    21661 agacaatcct gccgctgaag ctttaccagg ttaccgaaaa atgaatccga tggtttattg
    21721 tggtctttat ccaattgata cttctcgtta caacgatttg cgggaagctt tagaaaaact
    21781 gcaattgaat gatgctgctt tacaattcga accagaaacc tctcaagcat taggttttgg
    21841 gttccgctgt ggattcctcg gacttcttca tatggatgtc gtccaagaac gcttggaacg
    21901 ggaattcaat ctagaattga tcacgactgc accatccgtt atttaccatg ttaataaaac
    21961 ggatggtacg actgctacag tagataaccc ggcagatttt cctgaacctg taacgattca
    22021 ggatgtagaa gaaccattcg tcaaagcaca gatcatggtc ccaaatgatt ttgtcggtgc
    22081 agtaatggaa ctttctcaac gaaaacgggg agaattcatt acgatggatt atttagatga
    22141 ttaccgagta aatgtcgtct ataatattcc gctttctgaa attgtctttg actttttcga
    22201 caaattgaaa tcaagcacga aaggctatgc ctcattggat tatgaaatgt ctggttatca
    22261 aaaaagcaag ttagtgaaga tggacatctt gctgaatgga gaaaaagtcg atgcattaag
    22321 ctttatcgtt caccgagact ttgcctatga acgtggaaaa gcaatcgttg aaaaactgaa
    22381 aaaactaatc ccacgccaac aatttgaggt gccaatccaa gcagctattg gacaaaaaat
    22441 cgttgcccgt tcagacatca aagccttgcg taaaaacgtc ttggctaaat gttatggtgg
    22501 agatgtttct cgtaaacgta aactcttaga gaaacaaaaa gaagggaaga agcggatgaa
    22561 acaaatcggc tccgtcgaag ttccgcaaga agccttcatg gccgttctga aaatggatga
    22621 gcaagatagt gtgaaataac ttataatcaa tcgaaaaacc tattaaataa acctttttga
    22681 gtagatgcaa cactgaggat tatctgtata aaagatttta aactgtcatt tggttatcac
    22741 ggcgatacta aaattaacaa aattggaata tttttcttga agatttttag ctgtcagtat
    22801 atgatgaaag catctcatag gaggtgcttt tttcatggtt gataattcaa gtggaagggt
    22861 tcttaaatct ctaagaaaag agaaaaaact atctcaaaaa aagttagctg atttagcagg
    22921 tatttcacaa agtactttag ttaaatatga aaaagggtca agaaaaatcc ctaaagacgt
    22981 agataatact ctaagcaaga ttctgaatat agaaactcta ataaaagatg aggaagataa
    23041 gattgagatt ctcatcggta aactaattgc atacagagat atgaataagt tacttaacaa
    23101 agagttagct gataatatag gaattagtga agtattactc agttatgtgt taaatagaaa
    23161 gagaaatccg tcaaaagaaa tgcaaaaaaa aatcgatatt tttctattaa gtaatgaaaa
    23221 agaaattttg aaggagatta atcgtgatag tgagatattt agtttatcaa aagacgataa
    23281 aattgttatg ggaaaaagaa tacgagaagt ccgaaagaac cgggaagaga cgcttgaaaa
    23341 atttggtaaa aactttacca tctatacagg caaaaatgtt ataagtagat gggaaaaagg
    23401 tattaatatt ccagatattg aaaaactaat gaatattgcc tatcttggta aggtcactgt
    23461 tccttacctt atgtacggag aagattataa gaatatactt ccaaaagatg aaagagttag
    23521 cgattttaaa aagataaatt cttttagtat gggattaagg atgaggaaaa ttaggaaaga
    23581 ttattatcta gaaagggagg agtttggaaa gcttttttct ccttcaattt caaaatggtc
    23641 catagatagg tatgagaatg gtagagatat acccaatact aatcgaatta tccaatatgc
    23701 ttatatagga aatctttctt tagaattttt gatttacggg atataaaaat agtttacgtc
    23761 gatacttatt aagttatcga cgttttttat tatagaaaaa atttataaaa ggaggtttct
    23821 tgatatgata agaaacaaaa agggtttgtt gaaagttgct ttactggcag cttttttcta
    23881 agtaactttt tgacaagctc aatggcaaca caagcggaag aagtaataca agaactaact
    23941 gttatgaata acagctagat aaaatgggct atgtatgtta tccaacaatt tagtaatcac
    24001 tagaagccta gcgaaagttc gcttgataag ttatagatga gtattgattt gtagtgtttc
    24061 caaatcaaaa gggtacagag ccacatgaaa tatgttgtcc tctgcagcgt caggatggga
    24121 atccatacca tggagattcg gagatctgtg agggaatact tacgtggtta atatgtcagt
    24181 tgtgttggac gtagtaatgg tcatagtggg tagattgact gaattcttat atgagaaaat
    24241 gaaaccaaca agaagatagg gttatttata cagaattggg gatcttttct ggaaaagtaa
    24301 tcggataatg cgagattcgt acggtctttt ttaaatttct ctaaaaaaaa cggcatcgaa
    24361 tgggttgggt gattgataag caatgaatca tagactgata tagaataaaa aatgattcta
    24421 tttatctttt tacatctatc gttttttcaa gtagccgcaa attatatcag cattcaacaa
    24481 tttttgtata caagttctag taaaaggatc tgaagaaaaa gttggttgtt gtgaagataa
    24541 aaaaagaatc aggttttact gagtttttct tcaaaatttg tgttattagg ttaggacaaa
    24601 gataagaaga agtatgaaga tttaaaaaag gaatgaggaa aagatagtaa tttccgatta
    24661 ttggagtttt tttatttata aagaagaagc gaataagttg tgattcctag atttcgcgcg
    24721 tgattaatta atgagcaaaa aatgcggaaa gttttagtgt ttaatacacg aggacaaagt
    24781 ttcttaggta ttgaaggcac acgtgtaacg gttgatgggt ggtgtgattg tttcgaggtt
    24841 ggaaaacatg ttgaacttat ggcatttact gagctaaaag ataagaacaa cgtggaattt
    24901 ttcgagagtg atattggttg ggacgatcat gaagaagttt atggaaaatt tattttgcgg
    24961 aagggaaatt cttatacgaa tggggaagca taagtagcga tttgttagag atcattgata
    25021 ggattgaaat tgtcgggaat atttatgaga atctagagtt atttgaggtg atagaatgag
    25081 tgatggtttt gaacaagtat caataattaa aaatcaagtt cgggagattc ttagaaaaaa
    25141 aggctatttg gtagatagtt attttgaagg agattacgaa acatgggtag gtgtctatgc
    25201 tcgtccagag aataaaccta cctatttaga tcctacgacg tcagaagatg cctatttaca
    25261 aaataggtat cgagtagatg gttttaagca agactttgcg gagtggtttg aatgggaaat
    25321 tgaaaacgga gaagtaaaag aagaataaga gtacttgttg tatcaaaata agccagtaga
    25381 acaaccaaac cagtagctag taatgacaat aatactgagg aatcggatca ttatggtatg
    25441 attgatattt ctcttccaga agatcttaag ccagatcctg taacaattgg caatagtgtc
    25501 atgatttttg attcattaga tgaagctatg tcatatggtt ggaaagaaat taaggatgaa
    25561 ggtagtaatt ggtatggcat gagatttggt gatggtgaag ttgccaaaaa agctggtatt
    25621 ccaattggga aatggacagt tgatttccac gaagcaggtt ctttttttgt tctgtaatta
    25681 tagtgggagg gtaaaatcat tgatgaaatg tgttcagata agagcgccag ataaaaatag
    25741 aagagacagt tcacgtcgtt atcacttttg gtaacggcgt tttgttttat tatataaaat
    25801 tctcaggaaa aggaggaatt cttgtgtatg caaaaacgaa aatgaattgg ttgaaagcac
    25861 tcgcattggt ggctttttct caattctgtt tcttggtggg aactgctcaa gcacatgcag
    25921 aagaaacgac ggctatacaa cctacgatgg aagccattgc ccaagtcgct tctgaagtga
    25981 cagaagtcca accagcagaa gccggggtcc cagctggtga agccactgtt gaacaggccc
    26041 ctgtgacaga agcgccaacg gtcaattcca aaacgactct cgatcagacg attggggaaa
    26101 cacaacaccc agggaatagt cctggtactt cacaaccagt tgaagaaact gcctcaatta
    26161 caacccctgc ttcagaaacg tccactctag aaggagaaaa agctgccgaa gcaacgcctt
    26221 ctgtgcagcc agagtcttct gagaaacaag cggaagaaaa gaaacgaata gaagaaacag
    26281 tggaacaacc agcggctact gctgacaaag aaggaaaaaa tgaggaaaac gttgaaaaaa
    26341 caatctcttc aaccgcagct agtcaaaatc aaccaacgaa accaaacaaa gaatctacgg
    26401 ctagtattga aaatcaaaag aaaaaagagc agaacattcc aaaaacaggt acacctacac
    26461 tgttagtaac agtaccaacc aatcgttcgg ttcgtgttcg ccgtgcaacc aatgcagctg
    26521 ttttaggtgg tcggaaaatt gattggggcg aaaatattgg tgttacttct agtccgaaca
    26581 atctaaactt cactttaaat tatatggttc gttttgagat taatggacaa gtggcgtttt
    26641 gtattgagcc tggtgtctca gccgcagaag gtcaagttta tacagctact gctttgaaaa
    26701 actatttgaa aaattcaaat gtacggaaaa aaacaagctt gatctcttat ttaggatatg
    26761 tcacaaataa agacaaatcc gatgaacaat acattgcggc acaatttatg atttgggaag
    26821 ctttgggcac aaaaatccca atcaaaaata tccgtgttga ttatgatcgt cgaaaagcag
    26881 aaattcaacg tttgattaat aactttggtg aaaaagcaac ttttaataat caaacacata
    26941 cggtaaaaat tggcgaaacg ttgaaagtaa agaataaaaa tgattttttg aaatatgtga
    27001 aagacattca aaaaccaaaa ggtgtcaatg tatctattca cggcaatgaa gtatggatta
    27061 cagtcacaaa agaggcaccg gataaagcaa aaattcaatt gaataaaatt aaagttcaag
    27121 gtgcgccatt agcctatcgt cgtccaggag ctcaaacaat tggtgtgtta catccaacag
    27181 aacctaatgc gtctgttatt aatttacaaa ttctaaaaca aggaaacatt cgtgtgttta
    27241 aagaagatgc ggaaactggt gtgaaagcac aaggtgcagc ttctttagaa aatgcggtct
    27301 atggtttgta tcaagcagat gggaaaaaga ttaaagaaat taccttgaaa aaaattaacg
    27361 gcaaagtaca ggcagaaatt aaagatcttg atcctggcaa atatgtttta aaagaaatca
    27421 aagcgccaaa aggttatgtg ctttctgatg aagcaattga aattgttgtt gaacctggaa
    27481 aaactgtcaa tgcgaccgca aaagatcaag tgatcaaagg aaccattgat ttagtgaaag
    27541 ttgctaataa agatttagtc gattcaacga atccagataa caaaccaaaa ttagctggca
    27601 ttgaaatcac tttgacgtca aaaacaactg gcaaagtggt gaaaaccgtt gtgacagaca
    27661 atgatggtta tgctactttt ggtaaaaata ccgttgtctt tgatacgtac attctttctg
    27721 aaaccaaagg aaaagaaggg tataaactat ttgaaccact tgaagtcaca atttctgaaa
    27781 atgggcaaac tttccattat gttttagaag ataaagtaat tgaacaacgt cttaaagtgg
    27841 tgaaagtgga tcaagaaaca ggaaaagtga ttccacttgc taatacagaa ttcaagattt
    27901 ttgatacttt agccaatcgt tatgtcacaa tggagattcc aaatgataca gaaacaacgg
    27961 atatctttaa aaccaatgaa aaaggctttt tcctaactaa cggtacactt acttatggaa
    28021 aagatcgcta tcgtttggaa gaaatcaaag cccccgtaaa ttacgttttg aatcaaaacc
    28081 cattagtgtt ctcgattacc aatgatacag aaccgatcaa agtgatccat tttgcgaacc
    28141 gtcaagcacg gaaaagtgta aaattattca agtatgaaga atggaacaag aagaaaacgc
    28201 cgctcgcagg tgtgaacttt accttgtata acggtaaaaa tcaagcaatt ggtgaatata
    28261 ccactgatca aaacggggaa attttagtga aaggtctagt tgccgatgac tattacttct
    28321 tggaaaatgc cccattagaa agtttccaac caaatacaga taaacaagca ttcacggttg
    28381 cattcaaaca aaatgcgcca gatgatgggg aaacgttagt ggtttctgta cgtaattact
    28441 tgattccacc aaaactagaa acacaagcgt caaataaagc cgatggtgaa aaagtggttg
    28501 atccattaga aacgatcgtc attaaagaca gtgtttctta taccaacttg tttgtcggaa
    28561 aagaatacac catttcagga gtattgatga acaaggcaac gaatgcgcca attttacaag
    28621 atggcaaaga aatcacaagt accttggcct ttattgcaga caaagtcaat ggcacaaaag
    28681 accttgaatt tgtggttaat gctagtgttc tgaaaggtca aacgatcgtt gtttttgaaa
    28741 aacttttccg tgatggcatt gaagttgcag cacatgcgga tatcaacgat gaagcccaaa
    28801 gtgtgaaagt gaataatcca accgttcata ccacggctgt ctttgaagac ggattgacag
    28861 tcgcagaccc aacaggattg tttaccttaa acgataccgt tgaaattacc gagattcttc
    28921 caggaaaagc atacttggtc aaagggatct taatggataa agcaaccggt gaacccttgc
    28981 ttgtgcatgg aaatacggta gaaacgagtg ttggctttat tgccaaagca gaaaatgaaa
    29041 cggtgatcgt accatttacc tttgatttga caggtataaa tggtcgtgaa atcgtcgtgt
    29101 ttgaaagtct ttttagaaat gatgaagaac tagcaaacca taaagacatc aacgacaaag
    29161 ggcaaacggt acgagtaact aacccaagtg tacgtacaac ggctacacat aaacccactg
    29221 gcttgaaaga ggttaatcca ttaacgaaag tcaccattac agacgctgtg aaataccacg
    29281 atttggtcgt tggaaaaaca tacacagtca acggtgttct aatggacaaa gtaacggaaa
    29341 aaccagtgct agtagatgga aaagaagtca caagtacctt aacgtttgtg gcagaaacaa
    29401 cggatggtac agtcaatcta gactttattc tagacgcacg tgcgttgcga ggcaaagaag
    29461 tcgtggtctt tgaagatctg tttagagaag gcgtattatt ggcttctcac gcagatatta
    29521 ccgacaatga ccaaaccatc aaaatcagta atccaaccat tacgacgaaa gcgacgaata
    29581 aaaatggcgg aaaagagatc ttggccttgc caaatcaaac tgtgcttgaa tgggtcaaag
    29641 tagacggtct tgcgattggt caaatctatc gcttaattgt tcaaggctac ttaacaccag
    29701 acggcacacc atttgaaggc actcatgcgg aaaaagtctt tgcggccgat aaagaaatga
    29761 tggaattctt atttgaattc ttagtggacg gtcgcaatct tgcaggaaaa ggcttgagtt
    29821 ttgcggaatg gctccaagcg aaaacggaag atgagaaaga aacgtttgaa gaagtggcga
    29881 aacacaatgt agatctaaca aacaaagatc aaacggttca aattgtggaa gcgccaaaac
    29941 caccgcaacc agtgaaacca gaacaacggg ttaaaccagc tactgttgta ccaatgttgc
    30001 caaaaacagg tgaacaaaca agtagtatac ttatggttct tggattagta gtattagtca
    30061 ttgctttagg tataacactt tataaagtac gtaaagccaa agattagtca tgaggaacaa
    30121 gcggttagga tttttaaccg cttgtttgat tgtaatgtag aaatatttag aatttatttt
    30181 ggtttttaag aggaggaaaa actttatggc agaaaaagca ttgcgtatga agaaagagtt
    30241 tttaggtcaa gtatttttat tgggttcacg ggaacctaat ccaccaaagg aaattaatgg
    30301 tgtgtctggg cgatatgttc aattacaagc agagtgttcg cgagaagaac agcctgcttt
    30361 ttttgtttat ctcacagctg aagaggataa aagattacga ttaccagaaa aagaggtgcg
    30421 tatttcattt gaagaagctt attttgtttt tcgtaaagaa cgagtgaata gtcgtgaaac
    30481 aaatgatatg ggacaaacag agcgtcgaac aactggctat caagatgttg gtgtcttttt
    30541 aactggtgta aaaattgggg aggttttgta agatgggatt aaaatttcca aataattatg
    30601 acattacaga agcattagat gaaacttgtt taggagccgt acgctttggc gcattagaag
    30661 agtctgatcg tgtatttgat aatgaaacag gcgattatac gggagaaatt cgatcacagc
    30721 gagcacaatt agtttttgga aatccagatg aaccaaagat tgcacgacga gttaaatttc
    30781 cagcagatgt agatttgact ggttttaaac gacgtgatcc tgtgaaatta attgatccag
    30841 aatatcatgt ggtatatatt ccagcacaag atggcaatcg tgaatattca gatttagtga
    30901 tttatgcgaa aggattagag aaagtagaag ctccaacaaa taatcctaat atgaaacaat
    30961 cacaagaaaa aggtttagat caaaaacaac caagtgttga ttctaaggaa aaagagaaaa
    31021 aataaggttt ttttgaaaga ataggaagga gtcttcctat tctttttatc taagatagaa
    31081 ggaaggggag attttgaatt cagataaaac caatcaaata ctttgagaga ttgtgcttgt
    31141 tatcaaaata tccaacaatt gattgagtat tttggagaca atccaacgat ttttgtacaa
    31201 acaaatgcat atatttgtgc ggttattggg cagtgtatgt ttttacgtga tcttgttcgt
    31261 ttttcattac cgtaccagtg gaaacaagat catcaggaag tgatttggat tcgtatagta
    31321 cgaatatgta atatgtgtgc acacccttat aaaaatcgat taaatctttt tcgattatgt
    31381 ttttttacta aatgtgatat tcctgtatta gttgcttttt gtcaaaagtt tttagcacag
    31441 gaaaagtaaa aacagaaagg aggaatgtga caattgaaag ctcatttttt caagtatcgt
    31501 ggtcggcgaa ttcgctattt caatcgttat ttatggtttt gctattatgg tttctttttg
    31561 tttccatttt tgattctgtt aggttggttt agttggaaag tattttatcc acgtttaagt
    31621 acgttttcga attggcaaat gtatcttatt cctggaataa gtattgtgtt gtatttgttg
    31681 ctggttagtg ggttaatttt agggttgatg cattggtcgc gactaaaaga aggatatttt
    31741 gcctctgttt attggcgtca gttacttgtt cgaatgttaa ttgacaatcg gtttgtttat
    31801 acaaagaaac aagttagtga gaaacaaaca agggaaaaaa tgagattacc aaaagtttat
    31861 ttttatcaaa agaaacaaga attagtggtt tcttttcctt tagatggtgg gcgttttcac
    31921 gaacgatttt taaaaatggg tcatttgtta tctgaagtat ttttgcgtga tttaattcgt
    31981 gaagaacgag aaaaagcccg aatcaattat gtattccttt cagattctgg tcggattcct
    32041 attgatcaat gtatagcaga aaatggacgt attcatttaa taaaaacttt ggattgggta
    32101 tatgatcaat caccaaatat gttgattgct ggtgcaatcg gtggtgggaa aacctattta
    32161 ttgtattctt taattcaagc ttgtttaagt gttgggacag tagatatttg cgatggaaaa
    32221 gcggcagatt tagcagcatt aggtgacgtc gatgtattca aaggacatgt gttttacaaa
    32281 ataaatgaag aaatgattat ttgtttacga aatgcattaa aagaaatgaa tcaacggtat
    32341 gcctatatga aaaccatgaa agatcctaaa tatgaaccag ggaaaaatta tgcttattat
    32401 gggttaccac cgcattttat cttttttgat gaatgggcag ctttttatgg ttcgttggat
    32461 atggaagcga gaaatagagt cgataaattg attcagccat tagtattaaa gggtcgtcaa
    32521 gcagggatgt acattatttt agcaatgcaa cgaccagatg cagaatattt tccaagtggt
    32581 gttcgggata atttaacatt ccgtatttct gttggtcgat tgtctcccat tggttattta
    32641 atgacttttg gagaagattt taaaaataag ccattttaca acaaagatca acctggtcgt
    32701 ggatatgctt ttccaggatt tgatgtacct gtagagtttt ttgcgccatt tgtttacaaa
    32761 gattatcact ttatcaaaga atttgagaaa tttactgaaa tgattgtcta tgattttaca
    32821 gtaaaagagg atagtcaaga tcaaacgatg ggagatgata atcagttgtt acaagaacaa
    32881 ctagagcgcc aaatgtattt agcaaaagat ccaaaaggaa caagtttttc tgatgcgcat
    32941 attttgtaat caactaagga ggtgaagtta aatggcagag agttgttatt atagagtagc
    33001 tacagcaata cgaatgatta atccttctct ttcttccaga acgttttatg attggttgaa
    33061 tcgtattgaa caagtaactg attaccgctt tttgcgcaaa gaacgtgttt ttacaggaaa
    33121 agttattaat caagtattgc taacgaaaaa agatatagaa cgattgactc gattgtatca
    33181 ttatcgagta gatttagaag aggatttaac tttgtcaatt tatcgcgttt tttctcctga
    33241 aaaatatagt gaaataacaa aattagatca tttaattttg tagttattat ctatcgggag
    33301 ggctctagac gtagtctggt agccgcactc tgcctggcgg tgcgcattaa ggctgcgaag
    33361 catgggccta taatgcgcac tcgctagcag cgtgtgcagc tcacaaagag gtcgtgcgct
    33421 tgcgcacggc ctctttgtgt aaagcccccc gatatataat atggggggca gaaataacat
    33481 aacacataga aaaaagtttt ataaaccctt ttgtatcaag tgattatcta aggtcagcag
    33541 ttgtactact tttcccaaaa aacgaggggg actagttgat atatcggtgt ttctaaaggg
    33601 ttaaaaaaaa tcgattttgt tattttataa aaggagcgag aaaatgacac cgattgagct
    33661 aaaaagctac agaaagaagt taagtgcaat tcataatata agtggagata aaaagccgct
    33721 atcacaagca agatttgcgc aattgatggg ctgctcaaaa gattacatta gaaagatgga
    33781 acaagggaaa agaaaaatcc cagaggattt tgaacaacga atcaaggaac gctttcgatt
    33841 gtatatgaaa agcggaatag atcttcgagc aatgattgat tatttgcgtc tttccttttt
    33901 tgggacagac tacaaaacag tgattcaacg tgtattacga atggatctga cagaatttga
    33961 aacacgtcca acgggcttgt atctttttga ctgtctttct gttcgtggga atatctgggt
    34021 gttctggcat tcaaaacgaa ctgtaacaga aaatgtactt gtgcaatttt caggacaagg
    34081 ttgccgggaa tatgaggaag tcatgcgtga acaacaaaca gattggcgaa catggctata
    34141 ttccatttgg aaaaatcaag gaaagttgga agctggttat acacgtgtgc aatgtagccg
    34201 attcgatatt gcgttagatg aactttgggg attggaaacg gaagaacatt tcgatctctt
    34261 tgaattacta gaaaaacaaa aagcaggttt attagaaatg gactttaaga gattccagaa
    34321 tattggcggt tgttttttag atgatggcta ttttcgaagt gaaggattat ctttgtattt
    34381 tggatcgcga aagagtcctc tcttttttaa tttttatgag aaacgttttg aaattgcgaa
    34441 ccgagaaaaa atttcagaat tagaagcatt aacgaaatat ggtatttata atcgatatga
    34501 gttacggtta gccaatgaga aagcaacaca agcagtagaa gcatttattt taggtgattc
    34561 accgaaacgt ttaggagaga ttggcgttgg tttaattaat gcaaaactta ttgtttggga
    34621 tagactggaa agtgatgcac ggattgttga taaacgatgg gcaaaattat ttcgttcgat
    34681 ggaagaattg gattttacag taaagccaga aaaattctca ttagatcgtt cagaacgctg
    34741 gtttttgaaa caagtagctc cgacattaaa gttgttacaa gaagcagatc gtattttagg
    34801 aagagacttt gtacaagaag ggattgataa tgccgaacta aatgacgagc aacaaaacta
    34861 tctagaatgg atgggaacgt caggagggaa aattgcatga atgcatatac gacaacagaa
    34921 tggctgcgcc tgttggattt aaaggctgca atcgaagcat taaatgaaaa gatggttgat
    34981 ttgtcttatt ttcgttttcg agttccttat attgaacaag cggtaaaagc tggacgttat
    35041 caggaaaaag agaactggca agaaattgca cgcttattag aagttcgtaa gggatacgaa
    35101 caagaactcg aagagttgga atttagccga aggaaaggaa ggctagaatt cattcgcttt
    35161 tatcgatttt cattgccaat acctgcgatt ttagcggtta aaaaaggctg cgataaaatg
    35221 aagatatatg aaaactgtgt ggcagcactt agcagcgaaa aacctcttat ggaagaaatt
    35281 tcattggtga ctgtcacttg ggaaatgagt cgccagccaa cagaagaaca gacatatctt
    35341 tctttagaag agattgagat agaattagag gaaattggtc gctatgccac ttgttctacc
    35401 tattgtggat cggtcattag tattgctggt gtgattgtct aaatgaaaaa aattatgatt
    35461 tatgttggtg cgtattggtc acgtgatcca acagtattag aaaatccaga ggcggtacac
    35521 tattgcttgc gccaattatt ctatctgtat aaagaacgat tagaatcgtt gattcgtcag
    35581 ttaccatata ctgacagacg tttagatgaa cttcttctaa ggtatccagc aatgtataaa
    35641 cgacgaaaaa atagattgtt accagaagag tatcccattg aaaaacatga acttgaaggg
    35701 cgatttgtgg cttatttcta tgatgatgtt cgtatgcgct tagtagaaca acgaatggaa
    35761 atagaaaatg atcgctacta ttttatcaat tattgtaaga agcgtaagta tcaagtaact
    35821 gatgattttt atgattgtat tttacaagat ggggaagtga ttctatccaa aattgctcca
    35881 agttttcgtg aacttgtgcc gattgatttt ttgaaaaaat gtcatttgcg gattcttccg
    35941 taaaaaagag ctataaaagg aaagaaaagt agaagccaaa tcggtttctg cttttttatt
    36001 ttttttagga aggagaagat ggtatgtcac tggaattttt aggagagcca attgaaaagg
    36061 aagagttcgt ttcgcattat atggatttgt ttattcaggt gataaaaaac tttgtgcgat
    36121 tgatgaattt tatccacgcg aacaagacta tctagccgct gaacgaataa aaatcagttt
    36181 attatgggaa aaattcacag atgaaaataa ccgaccgcca gattatcgct atttatcaga
    36241 ttgtttaacg aataatgtgc tttctaaaga atacctattt cacaaacgtg agaaaaatca
    36301 aatgtcggtt gaacattttt gtgattgtta tattgcatta ttgcgtagtc aacagttatt
    36361 aacagtagaa cgatatggtg cagagtttgg ttatgtcatg caagctgagc gtcatagagc
    36421 tacaagatgc tatgaaaaag aagatcaagt gattgatggc tatgaagaat tatgtattcg
    36481 aaacaatcgc tatttgcaac aacgtttata tgaacaattg atgatggatt tttcaaatct
    36541 ctatagaggg tatgaatgtt cgatgattca agaaaggtag aagaaaaggg ggaattttta
    36601 tgtggcgatt attatttttg gtggtatgtg gtatggcatt gttctatgtg tatcgtgtct
    36661 atctctatcc acgaatggaa cgatggacaa gttggtatag agatccttta aaacaacgaa
    36721 aaggattgaa gcggtatcgt tatgaattac ttgttgaatt ttcggatggg cgacaagcac
    36781 agtattattt ttttgcaaac tatccaaaat acaacaccag tgaactattg attcaattat
    36841 tcaaaaataa tccatatgtt gggttagaag aaaacggacg ggagttcttt tatcgttcag
    36901 ataatattag tgcggcgtct atgaacaaaa cacgaattgc ataaaaaatt ggaggaataa
    36961 tcatgaaaaa aacaacatta gcagtcgtaa caacactagg aatttcatta ttttcgtttc
    37021 aattagggag ttcattggtt tatgcagatg aaactgtaga aacacctaag tcagaaattg
    37081 tagaaacatt gccaggggaa ggattaccag atgttgagac agagctaaat ggaggaacaa
    37141 cggaaaaacc agcagaacct gaacaattgc agtcagagaa acctgaaaaa gagccagtga
    37201 caccagaacc agagtttcct gaatcaactt taccgactga ttcgatcgaa gaagaaggtg
    37261 tgcatagttc aacaacaacg gattcaagta tagtcacaac tccggaacaa tcaggaacaa
    37321 ccagttcttc tgaaaagaat actgattcgt caagtacgga acagccaacg atgtcgtcta
    37381 ccagtgaaga acaaaaagaa gaaccgaaac aacctgttga aaaatcagaa gcaattccaa
    37441 aagaaaataa acctattgtt ccagcaaaag aagtcacagt atcggtaacc ccaagcggtg
    37501 aaattacgac gaatacttct caaggaatga gtgtaccgat tgtcactagt aatgtagagg
    37561 aattgaccca tattccaaca ccaaccaccc cattaaaagt agcaggtgat caaacaattg
    37621 ttggtgtaaa agatggtatt ccattaatcc aagatagtga agggaatttg gtaaaggatt
    37681 tgtcgattcc tgtaaagaaa cttccaagtg gcaatattga aatcaaaaca gcagatggaa
    37741 aaacaaaagt cttaccaaag actggtgaag aaatacatgt tgcactttct gttctaggag
    37801 gagtgttgac aagtattgct ggatttgttg gatataaaag aaagagaaaa ggaatagaaa
    37861 atgagggtaa ttgaatggac tatttttcag aaaaacaagc ttatcgttgt caatcgtacg
    37921 tactagtaaa aacatactag aaggagtagc ttatgaaaaa acgacaacta cggcggaaag
    37981 aaaaagttta tacagagaaa gtaccgaagc aaaagaaaat ccattcaaaa aaagcagtga
    38041 aatggatttg gtgtttggtt ttatttcttt gtttttcagg gggcttggca ttttttcaat
    38101 ccatgaaaac gaaggttcaa gtgtcgtttt tgaaagatga aatccaacaa ttacaaaaga
    38161 aagaagtaca aagtcggtca gaattaacgt acacacctga aattgaatcc tttatgaatc
    38221 gttttgttgc tttgtatatg aatacattcg ataatcatga acaagaacaa caacgtcaag
    38281 aaacattaaa aaaagagttt tatgcacaaa actttattca agaaccagga agtgtttcag
    38341 gtgttcgaaa attaactgct gcttcgttta ttggattaaa agaaattgaa accattccaa
    38401 ctgctagtta taaagtaact tatgatattc gctcgatgaa tccagagagt aagaaagaaa
    38461 caagtgtgac aatcacacaa gtactcaatc ttcccttttc tatttcaaac aagggctgtc
    38521 gtattattgc ttatccttat tttacggcac taccaaagaa tacagaaaaa caacgaatga
    38581 ttacttatga tgacacatta tacgaaacgg ttgatgcgtc ggaagcgaaa gatgttgaag
    38641 gatttgtgga agatttctta gataagtatg cgaaaaacaa tgcaaaagat atgtcataca
    38701 tgatgaaaga acctgaagga ctcaatcaaa cagctaaagt tgatcaagta gagatgaaag
    38761 tattgcgtta taaagaaacg aaaaaacctt ttattgtaaa agcaacggtc gtctttcgag
    38821 atggtgatac gtcactttta caaaaagaac aaatgacgat tgtcattacg aaaaaaacaa
    38881 atcagtatta tgttgaaaaa ttaacacata attggttgga taggaaagga gaagaacgga
    38941 aatgacactg aagggaattg tagattttat attaacgcaa ggtggctatt ttttaataat
    39001 ggtggttgtt tatctgggat tgcgaaattt tatgaaatca aaagtaggaa atgcattaat
    39061 atctatcgca gcaggtgccg cagtttattt cttcttaaat gatccaacac gtgtatttga
    39121 tgcgtttggt aaaatcattg aaaaattatt tatggattag gaggatagtg aagtggattg
    39181 ggaaccttat gattattcga ttgtttatgc ggaaccttat cgcgtacaac agattacaag
    39241 caattggcgt cttccatttc cagttgcatt agtcaatgca gcaacttttt tgttggtcgt
    39301 aatctttgaa tatttatttt tacgttcatt tattggttgg gtcatttctt tttttccacc
    39361 agcaggagtt gttttttata taggactccc ctatttatgt gtgttagcac tggcaaaact
    39421 aaaacctgat ggaaaaaaag tgcattttta tttatgggat tggttttgtt atatgattcg
    39481 tatgatgaat ggtgattgtg tgtattataa ctgggacaag gtatcaaaca atgcattgga
    39541 agaattactt tttgtgaata agtatgttta tttagaggat tagaaaaagg gacaatcaac
    39601 tggttgttcc ttttttattg ggaggtgaaa ttcattggtg cttgtaaaag aaccagtaaa
    39661 agcattcaag aaaaattgtt tgttaaatgg agaggattgt atctgggcct actatcatgt
    39721 ttttccacga acaatttcaa aaaacacacc aaaaaagaca caggaaataa aagaaatgat
    39781 ggctcgttta ctgttgcttt tagcagatta tgaagatgtg gagttggtga tgtatccacg
    39841 aacaatgaat ttacggacga agtttgacac attaggaaag ggattttctc ccgatagtca
    39901 agcaattggt gcgtattatg caaaagaaac agtgacattg ttggaacgag aaggtcaagt
    39961 ctatcgtcct tcatttattt taggaatcaa agtcaaagaa aaagcaacgg tggagagttt
    40021 tcatgatgta ataaatctaa tctggttaga tttagcagga agtttagcac gcttatttgg
    40081 attagaagta gatgatttgg aaactttgtt ttctgaaagt gatccttatg taaaagaagt
    40141 gacacaatta ctttatagct tttctattcg tcccttagta aaaaaagagc tgttgtatct
    40201 caatcgtctg caatatattc gaggaatggc tcacaaagta gaagaacaaa ataattggga
    40261 agtactgaaa gacaatcaag gaacgtatgt acgtataccg aaggaacata aaggccttct
    40321 tcaattagaa gatgagttag ggagtagtta tttagcattg ttgcctattg gggaaacacc
    40381 tgaaaatatg agaaattgtg atctatttca atttgctcag gatctttctt tcccagcaga
    40441 attgcgaatg aaaattcact atccacgctt gaaaggacag ggaggtattg gtttaaaact
    40501 gaattggcta tcggaacggt tcaaaacaga gcataaggaa ttggttcaaa atgatgaaga
    40561 acctagtgaa cgcttattaa ctgttcgagg attagtaaaa catttaaagc aacgagtaga
    40621 taatgggcaa cctgtcgtcg attggatcgc atgttttatt gtatacggtc aaacggttga
    40681 agaagttcgt caaaatagtg caacggttat tcaacaactt catacgatta tccatatcca
    40741 ccgtgcaaaa aatgatcaac gagaattgtt ctataaatgt ttattagggc aaccattggg
    40801 acggcagtta cattggaagc accgaacaac tgtagaagca gttggcgaat ggttgttttc
    40861 agcagtatca gaattaggta tggaatccgg ctggtatatt ggtcggcaag atacattagg
    40921 ggatggtggt tctcgaggag aaaaaacacc attagaagaa ttaatttatg cgtcaaatcg
    40981 ttttgtcttt ttgaatcttc tagccattgc ggaaggaatc aaaggtgcgt tgtttgatgc
    41041 gcctcatgta gcagtaactg gaaaaaccgg taaagggaag acctttttag ttggcttgct
    41101 ctttttgtat agttcgttta tgaatgtaca aagtttattt gtggatccca aaggagaaaa
    41161 gcgcaaatgg tttacgaaac tcattcaaga cccgtattat cagaaacatt accctttgtt
    41221 tgtagaacat ttgcaaaaaa tgagttatgt cacgttagat gctacaaatc cccgtaatta
    41281 tggtgtatta gatccaattg tttgtttatc ccgagtagat gcgaaacaag tagcccagga
    41341 tatgattgat gagttaagtc cgttaggtac caatcacctt ttaaaagggg cggtattaaa
    41401 gggaatccaa gaagttttag ttcaaaaaga agccggacaa caagttggct tgatgcatgt
    41461 ggtcgactat ttagaacaaa tggaacaaaa agaggttcga gaatatggtg agtttttacg
    41521 cttgacggta gaagattcga ttttacgttt aggattttct tatggagaaa acccgggatt
    41581 agactttaat gccaaaacaa cgattttaga gatccaagat ttgaaattac caaatgacaa
    41641 tgtccgacca gaactatata cagatgcgga tcgaaagtct ttgtgtttga tgattagttt
    41701 aggtcgtttt tgtgaaatgt ttggaaaacg agatagtagt aagaaaacag cgatttattt
    41761 tacagaagca tgggtattta ataattcgaa cgcaggacgt agtattattg cagcaatggc
    41821 acgtgtgggg cgttcccaga tgaatcaatt agtgttagat acacaattta ttggtgactt
    41881 aggttcagat aaagaaaaag ggaattttgg tgtcgtcttt gcttttgatg aagacagtga
    41941 gcgagaaaag attttgaatc atttacgttt ggaagtaaat gaagcgaact tagctgaaat
    42001 gagccacatg attaaaggac agtgttggat gttagatccc tatggacgtg taggaaaact
    42061 caatgtacac agtttgtttg aagaaatgac cgctgcatta caaacaacgg aaaaaacaag
    42121 tcactctcaa gtagaagaac aggatacgtt aatcatgcaa tagaaatgag gtgaaacaat
    42181 ggcttatata aagcataagg aaaagaaatg gtgcgttttg ttttttttaa cgattggatt
    42241 cggactgctt tttgttggtg gattggtgta tgcggcagaa aagcaggtgg atcaatcgat
    42301 ctatgagatt gggaaatttc atattgatac cttttcttct tatttatctg atagtgaatt
    42361 ttcattaggc aaaatgttat ttggggcaaa tggcacagat tttctaaaaa tgatgacgaa
    42421 tatgttcttt agtttaagta aattgatttg gcaaggcctt gattttgtca ttgagaagct
    42481 ctatgaagga gctgcaatga acgagttaat tcaccagttt ttcacttttt cggatactat
    42541 ttattcaaaa atatacgatc ctattggtgc ggggattgtc gtcttgtatg tgttatatct
    42601 atttactctt cgtatattta gagggaaaca ttacgcaaaa atggtgttta ttcgatttct
    42661 cttcgttgtc attttttcaa tggtttggtt tggctatggt agtaacgtac ctagtaaagg
    42721 agaagctttt accaaagggt taaatgattt gtccgtggag gtagaaggct ttattttttc
    42781 tgctacaaat agtctagatg aattggcgac aaaagatgtc aatggtgttt tagtgccaac
    42841 gacacaaaag gaagccattc aaaaagtgcg ccaactttat tataaagcgg ctgttgttga
    42901 tccgtatata ttgttaaatt atgggacaac aaatttagaa gaattgtatc aagcagagat
    42961 tgaaccacaa gaatttctag ccaaggaagc gtcaagtgaa gaattatctg atattgagac
    43021 aaaagtgacc actgctgcag gagaagatag agggaaaaag aaggcttatc gtgtctatat
    43081 tcaaccaact aaagctgttt ataaagcagt cgttggttct atggtgccat tgcttaatct
    43141 aagtattgga ttaccacttg tagtgattgg catgattcgt tttatctttc aaatgttggt
    43201 gttgtgttta ttacttgtga ttcctttttc attactcgct tcttttgttc cagtatttga
    43261 ttatatgctc atgaatacct taaagtcctt tttgggcttg atgtttcaaa aatcttttta
    43321 cagtgtgatt attttggtgg catttttgat ctataacata gtagacgatt taattccaat
    43381 gaatactgtt tttggttttt taggaaatat gtttgtcaaa gcactcctat caattttact
    43441 cattgtaaaa cggaaagatt tattaaagaa actagggtta ggagctgcag atcaaactat
    43501 aaacaacacg aaaaacatag caagtcattc tttgtctcaa atgaaacaac tgcctggtca
    43561 actatcaact gctggacaaa atttggcaat caaaggtgca ggattagcag ggaatgtgta
    43621 tcgagcaacg aatccactcg cttctgcagc gatacatgga tttcaaaagt ttcaacaaaa
    43681 acgtacacca caagaatcgg taaaagggag gcaagtggtt aatccctttg aactaggtgg
    43741 gcgttcaaat caagccttgc cttatagtgg tcgtcatgtt cgttatcatg gggtagagcc
    43801 tgaaatgtct tctaccaaat cgaaaacaga gtggaattct ggtctttcaa atggtttgat
    43861 tccaaattcg tccatgaatt cagtaagacc aaaacaacag gtacaatcat tggcacaatt
    43921 gagtaagcaa caagggaata atcaaagaaa accattgtta aataaaggta tcgcttttgt
    43981 tggtcaatcg attccgttgc cttctggaaa agtacccgtg ccacaattaa cagctgctgg
    44041 ttctctttca caacagattc gccaagtgaa aaatcaggcg aaaaatggga gttatccagc
    44101 aatgcagcag ttattgaacc aatactctgt tcctaattcg tcggtagcca ctctatcagg
    44161 aaatcgttct gtgcaacaac cggggcaacg ttcgtctgaa agtaaaccgt cgatttccgc
    44221 accaaaatcg ttacaacctg cattttcttc tttagataag ccaacagagc aatcggaaaa
    44281 aatagaaaag gatcctaaaa cactgccgat tttggacgca aaaccaggaa ctgattttac
    44341 tttaccaaaa ggacgaacag tccaaaaacc attatcgaag aaaggatgag gaagaaatga
    44401 agaaagggtg tctaggttgt cttcttttct ttattcttcc cttttttcta ttgccattct
    44461 ttttttcagg agaactttct gagcatggag gagaagctac aggtctagct gtcgctttta
    44521 atggggaata taccgaagga ttaccgattt tttcagagat taaaggacgt ggtccaatca
    44581 cagatgaaca tgcgcaagca gcagttggtg cagctgtaaa gtatcagtta ttaccgagtg
    44641 tgattctttc tcaattaggc tatgaatcag cttatggaca gtcacaatct ggaagaaacg
    44701 atacgaactt ttttgggatt acctggtttc ctggttgtcc ctacccacaa ggttctgcac
    44761 gtggaattgg tggttctgaa ggtggtttct atatgaagtt tccgaactta aaagcttgtt
    44821 atgactatta tggctttatg gtggcgagcc aaagcaactt taatcaatgt gtgggcaata
    44881 aagatcctgg aagttgttta ttgattctag gtcgtggagg ctatgcggca gcaggtatta
    44941 ccgaagggag tccgtattat cgaggagcaa tgagtatcat aacgtcctat aacttaacgg
    45001 aatatgatac ttttgcgatc aagcattgga atagcctacc aacaaaaaaa acttcgctta
    45061 ttgcaggaaa aagtaaaggc aatgcgaagg ttttggatcg gatcattgga cgatccgtaa
    45121 ataatgggga atgttatggt ggtacggcat ggtatgtatc tcaattaggt ggcccacagt
    45181 taatgggttc tggacacatg tttgcgatgt taatagggga agattataac tggacaacct
    45241 atggttggca agtaatcatg aacccaaaac caagtgattt acaagcagga gatgtggtga
    45301 attggcaagc aggtggtgca ttgtctcctg gaatctatgg tcacaccgga attataacag
    45361 atgtttcata tggcggacaa catttctcta gtgtggaaca gaatgccgag caaggacgga
    45421 ttatggctcg gtatcaacgc acgtacaatc aaacaaaaat tcgtagtctg gttcgtaagg
    45481 tgaaataaga aaggcagtga ctagaaacgt gaaaaaaggg ataattttag tatttagttg
    45541 gttatgtttg ataggtctta ctggttgtca ttggttttct tctgaaaaac aaaaaacaca
    45601 attgagaacg acagaagaaa caatcgaagc agtaaaagaa acaagtggaa ctcttcaaag
    45661 tactgaaagt aacagtcaaa ttggttcatt agaagacgag acagcgcaac aaatgcaaat
    45721 agatgaaatg acaaaaaatt tcttaaaaga ttttgctaga aaatggagta atttcacaga
    45781 tgtttataaa cgcaatcaag ctgtgaggga ctatatgaca gatcgttgta ttaaagacaa
    45841 tagtattgat gtagacccgc atgtacaaat gaaaacaact gggaaacttt accaggtgac
    45901 acaagatata gagaatccgg atcaatattt gttatttgga gaagaaacaa tgaataaggt
    45961 tactcaattt gtattacttc aattgaaagt agatcaagaa gctcaaaaga ttgatgcgat
    46021 caagatttat tatgtacgga gtgcgtatta aaataaaaaa tgaacactca tatttattat
    46081 acaccgaaga aatagaaaaa aacagactgt ttttttaaga aaaactatgt cattctttac
    46141 ttgaaaaaag ttgttatagg acagaaagga taaaacaaaa acggaaaaaa tatgtcttat
    46201 gaataactat aagaactatt ctatttttta ggagatggat acgagatgaa aaaactaatt
    46261 ggctatagtg tgatgtgtat tggatttgtt ggtgtgggat tgtttgtatc gactgaatct
    46321 gcttctgcgg ctgaatggta taatcctatc tggtataatc cgaacattga atgtcaaaaa
    46381 ggctatccgt atggaccgac aaatctgaaa aatggaaaat gtagagtaaa ttggaatggc
    46441 gttgtagata gtgtaattac tggttcaatg gactctgctc tacatggctt tacgggtcac
    46501 tagaaggagg aagaaaatgt ctgaagagaa aaaagaaatc gttgaatcat tagtagcttt
    46561 aaaagattct ctttctaaga ttgaatatgt tgataggcaa gaaattcaaa aactaattga
    46621 tgatactatt attgaaatcc aagatgcgcg ttgtgaaggt ataaaaattt ctgtagcttt
    46681 atctaaagta atagaaaaaa tgaaccgttc attagcgttt aatggattga agctagatag
    46741 acaaactagt ctgatatggg atcatttaaa agacttgtat gacaagtcta aaagaagtga
    46801 acggactgct gtatcaatct taaaaggcct ttggggaatg aattcataaa caagatattt
    46861 aaaaagtaac ttatttttta tggaggagaa attatgaaaa gaataattaa aatatttggt
    46921 gttttaggtg ttttcttatt aggtttaagt acttttgtta gtactggaac aagtgtgtat
    46981 gccacagaaa aagtgaatac agttgattta gaaataaaga ataaattagt agatgaatta
    47041 actttttatt ttgatacagt tggtttttta gacgaacatg gtgtatacca tataaaggat
    47101 tttggtgcta ttcgtgaacg aatcagtcaa gataacgaac aaggaagagc agcaagagtc
    47161 ttttatgaac atattattgt tcctagaatg gagaaagttg attggaaacg atatgctgga
    47221 tgtgtagcca ttaattctct tcctggagga ggagtggctt gggctatagg tacgcaggga
    47281 gattttattg aagcattaaa agcttttaat tttgaaaaag ctgcggattt attattaaaa
    47341 attgcagaaa aacaattagg aaaagctgca gctaaagagt tagctaaggt aaatattgta
    47401 gcaggaattg cagcagctgc tgctagctgt gcattagagt aaaagtttat gaaaagttta
    47461 gaacaagagc atatggcttt taaacaagca atgtttaaag aaaatatcta tttaaaccat
    47521 aactatattc gtgtttctaa ggcatgttca ccagtattga atatgcttgg aggtggaaat
    47581 ggattatatc atttactgtt tgtcgatgtt tgttggttaa tttttttacc agatgaacta
    47641 gttatagtca atgaaaaaat aacaagtaaa aatgaagtgt ttaattattc tttaacaaga
    47701 attaattaca aagagattac caagttttca ctagaaaaag ttcctttttg gggagaattt
    47761 tgtttaaaaa ttaaatgtaa ctggaaaaga atgtatttct atattgatgg tgatgatgct
    47821 cttacattcg gtaaaactac tttttctagt tttaattttc agtttctatt aaaaaataat
    47881 ttttatggtt tattgaaata aggttgcgaa ggaagatgta gagttatttg cactgttgtg
    47941 ttggtatatc tcttaatgct gtgtcttttt ggggagatta attagtggag tgattatatg
    48001 agtatagatg attgtttaga agaacaaata agattatttt taaagttttt aaaagttaaa
    48061 gttcaaaata actacttcac atgttatagt tatgcacacc ctgttttaaa tagtcttcct
    48121 tttaattctt tagtgcaagg attgattaca aatattgttt tattagtctg tacaccaaat
    48181 gaaatatttg taaaaaagat aggcagtggt atttcttcag taagattaaa agagaaagaa
    48241 tatactcaaa agattattca tattcctaag cctgaaatta agcgttttga gataaaagac
    48301 tattctgctt tagggctgtt ctcaattggt tactggtgat aaaaaataag aatactaaaa
    48361 ctatacgtag aatataagta atcggcatca atcctaatta tgtgattgaa tgccgatttt
    48421 tgttacgcgc tatttttaat tatctgtaag aagaat
//
LOCUS       DACECY010000017        48114 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.018, whole genome shotgun sequence.
ACCESSION   DACECY010000017 DACECY010000000
VERSION     DACECY010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.018"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..334)
                     /locus_tag="IWO38_000997"
     CDS             complement(<1..334)
                     /locus_tag="IWO38_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313101.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFE"
     gene            complement(509..2644)
                     /locus_tag="IWO38_000998"
     CDS             complement(509..2644)
                     /locus_tag="IWO38_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0313102.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(2727..4382)
                     /locus_tag="IWO38_000999"
     CDS             complement(2727..4382)
                     /locus_tag="IWO38_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0313103.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(4384..4698)
                     /locus_tag="IWO38_001000"
     CDS             complement(4384..4698)
                     /locus_tag="IWO38_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0313104.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            4868..5128
                     /locus_tag="IWO38_001001"
     CDS             4868..5128
                     /locus_tag="IWO38_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ0313105.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            5142..5456
                     /locus_tag="IWO38_001002"
     CDS             5142..5456
                     /locus_tag="IWO38_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ0313106.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(5941..6495)
                     /locus_tag="IWO38_001003"
     CDS             complement(5941..6495)
                     /locus_tag="IWO38_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ0313107.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(6621..8549)
                     /locus_tag="IWO38_001004"
     CDS             complement(6621..8549)
                     /locus_tag="IWO38_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ0313108.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(8855..9571)
                     /locus_tag="IWO38_001005"
     CDS             complement(8855..9571)
                     /locus_tag="IWO38_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ0313109.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(9572..11395)
                     /gene="glpO"
                     /locus_tag="IWO38_001006"
     CDS             complement(9572..11395)
                     /gene="glpO"
                     /locus_tag="IWO38_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ0313110.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(11409..12905)
                     /gene="glpK"
                     /locus_tag="IWO38_001007"
     CDS             complement(11409..12905)
                     /gene="glpK"
                     /locus_tag="IWO38_001007"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ0313111.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(13041..14501)
                     /locus_tag="IWO38_001008"
     CDS             complement(13041..14501)
                     /locus_tag="IWO38_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0313112.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(14491..15786)
                     /locus_tag="IWO38_001009"
     CDS             complement(14491..15786)
                     /locus_tag="IWO38_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0313113.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(16118..16795)
                     /locus_tag="IWO38_001010"
     CDS             complement(16118..16795)
                     /locus_tag="IWO38_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ0313114.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(16825..17916)
                     /locus_tag="IWO38_001011"
     CDS             complement(16825..17916)
                     /locus_tag="IWO38_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ0313115.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(18017..18451)
                     /locus_tag="IWO38_001012"
     CDS             complement(18017..18451)
                     /locus_tag="IWO38_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0313116.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(19035..20492)
                     /locus_tag="IWO38_001013"
     CDS             complement(19035..20492)
                     /locus_tag="IWO38_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0313117.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENL
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIEDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(20489..22384)
                     /locus_tag="IWO38_001014"
     CDS             complement(20489..22384)
                     /locus_tag="IWO38_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0313118.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(22442..24217)
                     /locus_tag="IWO38_001015"
     CDS             complement(22442..24217)
                     /locus_tag="IWO38_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0313119.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYLVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESSEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(24267..25763)
                     /locus_tag="IWO38_001016"
     CDS             complement(24267..25763)
                     /locus_tag="IWO38_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0313120.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     EIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRIWKEEDCAPVMLDGDEQYLQANTVDYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(25957..27579)
                     /locus_tag="IWO38_001017"
     CDS             complement(25957..27579)
                     /locus_tag="IWO38_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ0313121.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(28029..29342)
                     /locus_tag="IWO38_001018"
     CDS             complement(28029..29342)
                     /locus_tag="IWO38_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ0313122.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCILMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLLGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFMGFMWILNVK"
     gene            complement(29587..29811)
                     /locus_tag="IWO38_001019"
     CDS             complement(29587..29811)
                     /locus_tag="IWO38_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313123.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(29831..30199)
                     /locus_tag="IWO38_001020"
     CDS             complement(29831..30199)
                     /locus_tag="IWO38_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0313124.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGVLT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(30395..31105)
                     /locus_tag="IWO38_001021"
     CDS             complement(30395..31105)
                     /locus_tag="IWO38_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313125.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGDESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(31123..31619)
                     /locus_tag="IWO38_001022"
                     /pseudo
     CDS             complement(31123..31619)
                     /locus_tag="IWO38_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(31656..32108)
                     /locus_tag="IWO38_001023"
     CDS             complement(31656..32108)
                     /locus_tag="IWO38_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0313126.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(32243..32848)
                     /locus_tag="IWO38_001024"
     CDS             complement(32243..32848)
                     /locus_tag="IWO38_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ0313127.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(33298..33918)
                     /locus_tag="IWO38_001025"
     CDS             complement(33298..33918)
                     /locus_tag="IWO38_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0313128.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(33920..34789)
                     /locus_tag="IWO38_001026"
     CDS             complement(33920..34789)
                     /locus_tag="IWO38_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ0313129.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANKAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(34812..35219)
                     /locus_tag="IWO38_001027"
     CDS             complement(34812..35219)
                     /locus_tag="IWO38_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ0313130.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDRHKLAELYEQVGLKFSELHENDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(35238..36527)
                     /locus_tag="IWO38_001028"
     CDS             complement(35238..36527)
                     /locus_tag="IWO38_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0313131.1"
                     /translation="MSVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINDQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     ILLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(36554..36856)
                     /locus_tag="IWO38_001029"
     CDS             complement(36554..36856)
                     /locus_tag="IWO38_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0313132.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(36881..38974)
                     /locus_tag="IWO38_001030"
                     /pseudo
     CDS             complement(36881..38974)
                     /locus_tag="IWO38_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
     gene            complement(39463..40191)
                     /locus_tag="IWO38_001031"
     CDS             complement(39463..40191)
                     /locus_tag="IWO38_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ0313133.1"
                     /translation="MGLCLFALPTQGFAVAGGHGGGTSSGRSSGRGSFGGRSYGGSYF
                     GSRSYGRDSYGYYGPSTGPSSYEQTGFLALAVLSVGGVSAFSIVKNKRQKKRLKSEIL
                     SRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFAEHQKVLQKNKAD
                     GVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLISGSKTQRQYFSQ
                     IWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(40337..42958)
                     /locus_tag="IWO38_001032"
                     /pseudo
     CDS             complement(40337..42958)
                     /locus_tag="IWO38_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298916.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            complement(43072..44403)
                     /locus_tag="IWO38_001033"
     CDS             complement(43072..44403)
                     /locus_tag="IWO38_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0313134.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRVMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKANFGQLNELVPFGFAGMIASFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKNAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQSGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFVGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(44369..45847)
                     /locus_tag="IWO38_001034"
     CDS             complement(44369..45847)
                     /locus_tag="IWO38_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0313135.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLKNPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(46152..47987)
                     /gene="tdc"
                     /locus_tag="IWO38_001035"
     CDS             complement(46152..47987)
                     /gene="tdc"
                     /locus_tag="IWO38_001035"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ0313136.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEQVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     EQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKEIAKAMEKKLNEIIQ"
BASE COUNT        13745 a        10351 c         8034 g        15984 t
ORIGIN      
        1 attcaaaaga tttatttact tttttgtata gatttcgatt gttccaccta gtcaatacaa
       61 atgaggtaat caggaaactg aaaaaaatca atacaaaaaa gccgattaag aaatacagca
      121 ttttcttttc tgataaaaaa gcgtacaagg aaaaatcctt ggctctttct ggtctcgtta
      181 atgggaaaaa gacccatata aaaggaagaa caacgatcat ccatcctaaa agagccacta
      241 acgtttgcca aatctttaac cagtaattac cttttgaaaa atacttatct ttcaccaaaa
      301 ttgatgcaac ttcttctctt gttacttttt tcatatcctc acctcacgtt tttttgttac
      361 gacgtaggag tttaccatac atttcatttc ttttcacata aagtgttcct aaatttttcc
      421 taagaaaaag aacaaaacat ttctcatcag caaatgaaaa atattttgtt ctttttatca
      481 gttgttcttt ttatttttta tctcttaatt atgttgctct ttccaatacg acaatgcttt
      541 tcattcctct agatatcgcc gtgtacattt gtctgcgtct tctttgctca tctccatcgt
      601 tttcaaatgg atataagatt acatgatcaa attccagtcc ttttgctaga ctcaaaggaa
      661 tgatttggca gttttctaat ccatatgaat tttttttaat cgattcttgt agcgtttggg
      721 tttcctcttc tgtttgggtg ataactgccg ctttttctcc ttttagagaa accaaaatct
      781 cagctagtat tttcaacaat tcttcagagg atcgaaaaga gcggtattcg ggttcttttc
      841 cttgcggtcg gatcgatacg atgtcgatct tctcttgatc cacagcatag gttttgaaaa
      901 gctctgtgat tgctccgcta gaacggtaac tatttctaag atcataccga tggatcggca
      961 gatggtagtc atcgaaacaa cgcataatat cagcaaaagt agtggaagaa ttaaagattg
     1021 cctgattctc gtcacctact aaagtaaagt gagtttttgg aaataattcg atcaatagac
     1081 ctaattgtgc cagggtataa tcttgtactt catcaatcaa tagatagcga aatgctggta
     1141 catttacctt ttcaacaagt aaatgacgaa tagctaatat aatgactgct tcatcaacag
     1201 tacgtgtggg ttgccaagca taagctgagc catatcgttt ttcataaatt ctttcaaaca
     1261 actgatgctc atccacccat gccatttctt cgatttgtct ggttattttt cgatattttt
     1321 tttgtaaaag tttccttgca taagctacaa tactttgttc cgaatcatca gaaatcaatt
     1381 taccgaataa ttccaactgc ctatcttctg tcaacgagag tacctgattc tgcacagcag
     1441 gactttttgc ttgtttcaaa agatgattct tccattcgct catcagtgcc tttttcgttg
     1501 cttgaagcct atcgatcaat ttaggatgag caggaacttt ctcatatatt ttttcgatcg
     1561 tttttttaga aaggactgtt tttccatttt tagtaagtcc acggataaaa ttcggatgat
     1621 ctaaaaacaa aggatctgat tgttttagat tatcgataaa ctttttgcta cgcaaacgtt
     1681 ctgtctgctc cgatacattt tctcccgtga tacgtttgaa ataagcctct tcaccttcta
     1741 tttcctctgc tgaaagctgt gaaaccagct gtatgatggt tagattcagt ggatttcttt
     1801 cacctaatga tggtaggaca ttcgaaatat agtcgataaa ccgatgatta ggtgatagaa
     1861 tcagcaagtc atcggatgtc atcgtatctc gatataaata gagcagatac gcaatacgct
     1921 gcatgatagc agaggtttta ccacttcctg ctacgccatt taccaagata ttttgacttt
     1981 tcacatcccg aatgattgtg ttttgttccg attggatcgt agcagtgata gccttcattt
     2041 cattggtctc atcttggcct aagacttcta ataatacgtc atcttgaata gctacagacg
     2101 tatcaaaaaa cttgaccaaa cgatctcttt ccaaaagaaa ttgtcgtctt tctttaataa
     2161 ccgtatcgat tttattttga cgaaccgtat aagaagatgg accaatcatg ttattgtaaa
     2221 aaagttcggc aattggtgaa cgccaatcat aaacaagatt ttcatctgca tcatctgtga
     2281 aatttgctgt accgatataa aaggattctt ttggctcatt atccagaaag tctacaacga
     2341 cttttccaaa gtagggactt ttcaataaac gttcgatctt tgctagctga ttttctgcgt
     2401 ttttctgttt gatattcatc tgatcgattt ctctattttt catttcaatc atcgaaaaag
     2461 tatccaggtt atctaagtaa ctgtcaaaat tcaatcgaag ctcgccagcc atttctttca
     2521 acgactggtt tccttctatt tttgccgtat ccatcgattc ccgcagttcg ttttttttat
     2581 caattagctt ttgatacacg ttcttcagat atgcttcttc taactgtttt tgtgtgttga
     2641 ccatacacat tctcctcttt ttttaaagtg gtggaaccga ttagtttacc tcttttaaga
     2701 gtcgaatgca agctttttaa cgagtatcat ttgttaggat atatcagttc ttcaggtcgg
     2761 acagtttgat ataaagaaaa tgcaccatca aggtttttta cgtggtagcc agactgcttc
     2821 aagattcttt ccgctagata gctacgtaat ccgctatgac agctgacgat atattcttgt
     2881 tggctgtcta attcatttaa tcggtcccga agttcattca aagggatatg tttggcttcc
     2941 ttgaatttgc ccttttccaa ttcttctgct gttcgcacat ctaataaaat ttttcctttt
     3001 gctagttctg tcggcaattc atgccattgt acggtttcac taagcccttc tgcaatgttc
     3061 attgctgcat atcctagcat gtttactgga tctttggctg agccaaatgg cggtgcatac
     3121 gtcaattcta attccggtaa atcgaagatc gtcaaatgcc ctttgattgc tgttgctaaa
     3181 atatcgatcc gtttatccac accttttgcc ccaacacctt gtgcaccata aatctcgcca
     3241 gtctctggat ggaagatcaa cttcagtaaa atatctgttg cttctggata atagcttgca
     3301 tgatcttttc ctgatacatg aatgacttca aaagacagtt cgttttcttt ggctgttctt
     3361 tcacttaaac cggtggatgc ggcagtcatt ccaaacgttc gtataattgc tgttccaata
     3421 ccgccttgat ttcttcgaga aattccggaa atcgtgtccg caacttgtct accttgacga
     3481 ttggctggtg aagcaagaga aataagtgca tcttttccag tgatttgctg ttttacaacg
     3541 atagcatccc ccaccgcata aatatcagga atattcgttt gataacgttc gtctactagg
     3601 atacccccgc gtatgcctaa ttcgattcca gcggtcactg ctagctggtt atcaggacga
     3661 acaccaatgg ataaaataac taaatcagca gggatggtac gcccatcatc cagtcgcaat
     3721 cgtttcccgt ggtcttcaaa tccagcgaca gcatgagaag taatcacttc tactccttga
     3781 tgagacaatt ctttttctat aaaagctgcc atttcttcat ccagtgttgg aagaatatgt
     3841 ggtgcttgtt cgatcaccat gacttctaaa cctcttcttt ttaggttttc tgccatttcc
     3901 aatccgatga atcctgcacc aacgatcacg gcacgctttg gctgtttttc taatgcatgc
     3961 atcactttat cgatatctgg tacatttcta atagaaaaaa cattgtcggc ttctgttatc
     4021 cctggaatcg atggaacaac tggttttgca ccaggagata aaaccaatgc atcgtactgt
     4081 tcaatgtgtt tttgaccctc atgtacgact tctaccgttt tgttttcggg aaagatggct
     4141 gtcacttcat gatttggacg cacatctaaa caaaaacgag cggataatga ttctggcgtt
     4201 tggacaagaa gattttcccg ttcactgatt tctcccgaaa gataataagg caagccgcaa
     4261 tttgcaaaag aaacatacgg tcctttttca aaaacaacga tttctgcatc ttccattaat
     4321 cggcgaagcc gtgtagcagc agacattcca cctgctacac ctccgacaat aacgattttc
     4381 atcttatttc cctcctactg ttcgatcaaa ccattgattc atgccaccac gtacattcac
     4441 tacatcataa ccagatttct ttaattcttt agctgcttgt ttactcctca tccctgattg
     4501 acaaatcaca tagacggtct tttctttgtc tccatcatat ctgtttatac tttgtaatgg
     4561 aacattttta gctcctttta tatgcccgcc ccggtattct gcaggtgttc taacatcaag
     4621 taacgctgga ttttccaaaa gcttttcctt taattctggg actgttatcg attttattga
     4681 ttgaaatagc aaattcattt tctgcacctc atttacattt ttataaatac ctcatggggt
     4741 atattaaaca ggagctttat taatatgtca accagaatga ctcttcattt ttttcactac
     4801 tttttatatt tgcataaaat accgataagg ggtattatct aattataaaa atagaggaga
     4861 atgtgttatg gagattgata ataaaaatat cctgaaccgt ttgaaacgta cagagggaca
     4921 aattcgtggt atccaaaaaa tgatcgaaga cgaaaaagaa tgtatggatg tcatcactca
     4981 attaagcgct gttcgttcta gtattgatcg cgtaatgggc atgatcgttg cagataatct
     5041 gaaaaattgc tttgagaaac ctgatagtaa tccagaagaa caagcggcta aactagaaca
     5101 agcgatcaag atgattatta aaaagtaaag gaatgatttc aatgaaagtc gttttccata
     5161 ttgatgaatt agaaaaatgg tcagaaacag ggaaaaacgt aaaaaacctg ataaaagctt
     5221 ctccagaaac gacgattgtc gtatctgtaa atggcatcgc gattaccggt tatctagact
     5281 ctgccaatgc agaattctta gatttgcaag gagtcgtttt tcacgcttgc gccaatgcta
     5341 tgcgtgctaa tcatatttca gaaagttctt tacctgaaca agtaatcgtt gtaccagcag
     5401 gtgtccttga tttagttgaa ctgcaatccc aaggttatgc ttatatcaag ccttagtcag
     5461 tagaggttgc gacagaagtg atcagcttca agaaatttcg gcttattttt cgaagaagct
     5521 acttctggaa aaccgtgaat caaaggttgt gaaaacgctg tcctgcatca agtaataaga
     5581 acagccaaac aaaaatagct tctcatattt ttcgtttggt tgggcttatt accgcagatg
     5641 caagcttttg aacaccgttt aaggaagagg ttgtgaaatc agcggtttga cctaagtatt
     5701 agagaaaaaa acgggaaaat agctcctcat attttgccgt tttttgagct aataccgaag
     5761 gtcagctgat tgaacaccgt ttaccaagag gttgtgagcc taccatttaa ctccgaatca
     5821 caagaaataa tttcaaaaac aacttctcct gtttttggtt tggttggctt attatcgaga
     5881 agtagctact gggagtctat ttatcaaaaa aaggaatgcg acataggtca cacccctttt
     5941 ttattttctt tgaaacatct ttcggtactc gacagcatac aagacaaaag attgtaaaag
     6001 accgtaagtg atgacggcaa aaatgactct cacccacagc gttggtaatt ctgggaaaag
     6061 cagcaaaagg atcatgccaa catagatgac agcagtcgct actttgccat accacttcgc
     6121 tccacttaat ttgatatttt tagatagccc gattaatcca gctacaaaca taaagccttc
     6181 ttttatcaag aatagaccaa acaacagcca aagccacggt cgataccatg ccatagacaa
     6241 aatcagaaaa agttgtgtca atttatctgc aaacggatca agcaccttcc ctaagtctgt
     6301 tacctgatta tatctccgag caatgacccc gtctagataa tcagacaaac ctgagacaac
     6361 aatgaccgct cctgctatat aaaatgactg tctcagcaca aaataaagat aaatcggcag
     6421 caaaaaaata cggaatatcg ataaaagatt cggaatcgtt tgtatttctt tacgccagtc
     6481 tgtgcttaat ttcattttat cgccctacct cctcagcact cttttctttt tattttagct
     6541 tacacgggga ataaaaccaa tgatttcact gtttttattt ttctttacag ccactgatat
     6601 tatgagctaa tttgagataa tcacttgctg ttttcatcgt ttactcttct ttcttcctgt
     6661 cgtttgcgca tttcttttac tagtacttta tgagcttttt ttgatcctcg ctctaggtca
     6721 cgctgccgtt tacgtgtttt ataatcgata aacagactgt ctaaatcgta acctgtcggg
     6781 tacagatctt ctgctgaacg caccagacga acacgctgac gaggcacgtg gattattttt
     6841 ttgtctaaat atacatcaat cgtatcttca cctatatctt cataaactaa cgcttctttt
     6901 tggtattcat tgacaaatat ccgatcacct tttgcaaaga gctggcgttc ttttttattc
     6961 tccttcctat ttgtaactgc tgtcttagga agaaattctt ttttctgtgt ccgataggct
     7021 ttttccgcca agtaacgctc tgcttgatgg atcaatttca tcgacatatc cattttatga
     7081 gcaatccaca aagcttggct ttctcctgtt tctccaaccc gcaactgata tttaggacga
     7141 agtgcttccc gatcgaaagc catagcagcc ggcacaaaat cttcatgatc tccggcgaat
     7201 ttcttgattt ctccataatg ggtcgtggca atgatcaatg ccccttgctc atacatagat
     7261 tccatgattg cgatcgctag tgcagcccct tcatttggct ctgttccact gccaatctca
     7321 tctaaaagta ccaacgtatt cctttttatc tttttcaaaa tagcagcgat gttttgcata
     7381 tgaccagaaa atgtactcaa cgcattttct aaattttggt ggtcaccgac atcgacgaaa
     7441 atctcatcaa agacagcaag ctctgtccct tcttttgccg gaacttgcat accgaacatc
     7501 gccatcaaag tcaataaacc gactgtcttc aacacgactg ttttgcctcc ggcatttgct
     7561 ccggtgataa tcaagcctcg atagtcttta ccgatttcaa catccaatgg taccgcatgc
     7621 tcaggtaaaa atggatgtct gccttgcttg ataatgatat gttccgattt attgattcta
     7681 ggtgtgcttc cattgatttc tcggctaaat tttgcccgag caaagatgat atctaacacc
     7741 gtgatcgttt cgatcaataa atcgatcact tcttcgtttt ctgcgatagc accagtcaat
     7801 gccgccagga cttgatattc ttcactgatt tcttcagctt ttaacaactg atagtgttca
     7861 ttcaatttag aaatcacagt tggttcaata aaaaccgtcg ttcttttatt tgattcatcg
     7921 ataatcgttc catctatttt atttttatag ctggctttga taggaatcgt atagtattcg
     7981 cctttttgaa caatcattgc ttcttggatc atttctttgt ttttcggatg acgcaagaat
     8041 ttcagtaatt ttgactggat ttctttttcg attagttgta attgtttgcg tactttacgc
     8101 aaattacgtg acgcgtcgtc actgacttta ttatttttta ttttctggtc aatcaactct
     8161 tctacattta tcaaatcagg aagatgtttg ctataagcaa acaacaaggg tgtctggtat
     8221 tgattcttat caaaaaactt tgtgatcatc cgactgcttc taagaaaatc cgcatattcg
     8281 atcaaatcgg ttggctgcag aatcaaccct tttttgactt gttctgtcag tgcatcgatc
     8341 ctcggcaacc ccataaatgg aacatgctga ctgctttcta agatcaagcg tgcttctctt
     8401 gtttcttctt gacgatccaa gacagtttgt aggtttgtcg agaccgtcat atcgctgatt
     8461 cgttttttgc tatagtttcc aattgctctt gcttggattt cctttttgat ccgatcaaat
     8521 tgtaattgac taatcatttc gttgtccata ttctctcctt ttcttgaaga gatacctgtc
     8581 ccaaaataaa taagggccta gagataaata aaaggataga acacagctta aaaaagtcat
     8641 gtcctatcct tattagaata caaagatatt gttttcctga caggtaccgt ttcataagca
     8701 caccaaataa actttgttta ttggctattc gacttactga ttagatacca gctacactca
     8761 caaaacatac cccattcttc taggtcttat ttattttcca cttaactata gcaagacaca
     8821 ttttggttgt caaactttag tatgttttat agatttacac cattaatgaa tagacaccta
     8881 ccgcgataat tgctccgatc attggacctg cgattggtac ccatgaatat gcccaatctg
     8941 aagttccttt attagctatt ggcaataccg catgtgctaa tcgaggacca aagtcccgag
     9001 cagggttgat tgcatatcct gttggccctc ctaatgaaag accgatggct aagatcaatc
     9061 cacctacagc gaatacgttc gttccaggtg caaattcgtt ttgtccaaaa gctagcaacc
     9121 caagaactaa tacaaatgta ccaattaatt ctgtgatcac atttgctgga taattccgta
     9181 tagcaggacc tgttgcaaac gttcctaaaa tcgcgctttc atctttcgtt gcattccaat
     9241 gtggcaaata agaaagccaa acaagaatgg cccctgcaaa agctcctaaa acttgcgcaa
     9301 tgatgaaagg aacgacaaga ttccaactaa ttgcccctgt catcgccata gctagagaga
     9361 ctgcaggatt caaatgagct gggcccatga atccagaaat atagacagcc attgttacag
     9421 cgagtcccca accaaatgcg atgacaatcc agccagaagc ctgtgcttta cttttgttta
     9481 aattcacagc cgcacacaca ccatctccaa gcaaaactag gatcagtgtc ccaataaact
     9541 cccccatgat ttgagtcata tcactgttca tttatttctc cccctttagt tcccgcagat
     9601 ctgattctgc aatcaatgct tctaaacgtt tagtctgttg cgccttctcc tcttctgtcc
     9661 attcaaaata tccagcaatt gcatcgatca caggttgctt gatctcatca agctgatctc
     9721 gttcaaacaa cagatgattt gtacgacgaa tgagataatc gcctggggcc aaaaccattt
     9781 cttcttctaa accataacgt aaccgagctg actcagcgag actcaaacca ggataaggtg
     9841 ccatttcttt tgctaattca aagatacgtc gggcattagt cccgtaaaaa tcagcgatat
     9901 aagtagcatc ttcttcctct aaaccggctg ctactccttc ttttgctaat tctgtcactg
     9961 tttcttctaa tttagttgga tcgaagtttc cacctgaaat ctgatatttt ttagagtcga
    10021 tttctttcgt ctctattccg tattcttctt ttaataattg gcgtatcaat cgcaaagctc
    10081 cttctgccat cttacggtaa tcggtgattt tcccaccaga taaagtcagc aaaccatctg
    10141 gttctctttc taaagaacta cctctggaaa tcgtagaagg tgctttttca tcacggctgt
    10201 tttccaaatg gttcaacacg tcttctactt cagcacgaga aactttgttt tccttatatt
    10261 cacttactgt atcgacaact ttattgaagc tcttgtctga aatcgatcca ttatctccac
    10321 cattgtaatc agaaccagaa ttaccaatca acagtggacg aagtcctgcc cagcttgctt
    10381 cgatatcatt caatgtgata tttgcttctg gatagcgatg attgatcaca tccaatagat
    10441 aatctacatc ttcttgtgtg actttcggat ccgtaaaatc tccttggtaa tccgtatctg
    10501 tcgtaccaaa gtaagtcttg ttttctcttg gaatagcaaa aaccatccgc ccatcttgct
    10561 ttcctgtatc gaagtatgtg ggttgcggta caggcagttt tttcgcatct acaactaaat
    10621 ggatcccttt agttggacgc attttaggag agactggacg cgtaaaattc aagtttcgta
    10681 ctttatctac ccaaggacca ctcgtattga tcactaattt tgctttgatt tcgatcactt
    10741 catctgtcag caggtcacga gctttgacgc caacgatttg atcgccatca tataaaaagc
    10801 caaccgcttt cattttactt actagataag ccccatcttc ggcagccttt ttgatattat
    10861 caatcactaa acgggcatca ttgttgcgga aatctagata gacacccgcg ccttttagcc
    10921 cttctttttt caaaaacggt tctctttcca atacttcttc tggtgtcagc gtataattct
    10981 catatttggt tcctgtcaca tttgccagtt tgtcataaag gtccattgct actttgaccg
    11041 agaacatatt gaaggttgtt gctccttcgc cctcatagat tggcaaaagc attggatctg
    11101 gttttgggat gtgtggggca atactttgta cgactgcacg ttcaccaact gtgtcagcta
    11161 ctacttccac atcaaatgtc ttcagataac gaataccgcc atgaacaagt ttggtcgagc
    11221 gagaggaagt cccttctgca aaatcctgca tttcgatcaa tcctgttttg attcctgatg
    11281 ctgctgactg gactgccaca ccggcaccag tgattccgcc accgatgatc aacagatcca
    11341 attcttcttg ctgcattttt tgaatgctat cttgtcttgt cttgtttgaa aacatatgga
    11401 tttcctcctt attcaaatgc ttgggtagct tttactgctt ttttccaacc gttgtaaagt
    11461 ttttctcggc gctcttcctc catttgaact tcaaatagct tgccagcttc ataaaattcg
    11521 cggatctcgt ctgtatcttt ccaataaccg acagctaatc ctgctaaaaa tgctgcccct
    11581 agcgccgttg tttccaagtt ttctgctcgt tggatcggta cattcaagat atctgtttgg
    11641 aattgcatca gatattcatt gtttgctgca ccgccatcta ctttcaatac aggaatcttg
    11701 attccagtgt catcttgcat cgtatcgatg atatctctca cttgataagc aatcgactgt
    11761 aaagtagctt taatgatatc ttcttttgtc gtaccacgtg tcaagccaaa catagcccct
    11821 cgtgctgcct gatcccaata aggagcacct agcccaacga atgcaggaac aacataaacc
    11881 tcatcttcgc ttgtagagcg tttcgcggcg tcttctgaat cgctagcttt ttgcaacatt
    11941 tgaagtccat ctctcagcca ttgaatcgaa gaaccagcca caaaaatact tccttctaaa
    12001 gcatagtaaa cctttccatt tatgccgtaa ccaattgtcg tcaataaatt atttttggat
    12061 aattgcggtt tttcaccggt attcatgaca ataaatgaac ctgtaccata agtattcttg
    12121 accatgcctg gttcgaatgc catttgtccg aataatgcgg cttgctggtc tcctgccatc
    12181 cctgaaattg gtgcttcgct tccataaaaa tgataacctt gcgtataacc ataaacttct
    12241 gagttagaag tgacttttgg taacatcact cgaggaatat ttagtaaatc taaaatctcc
    12301 tgatcccaat ctagctgatg gatattgaac aacatcgtcc gactggcatt cgagtaatca
    12361 gtaacatgtg cttgtccatc ggtcagtttc catactaacc aagaatcgat cgttccaaac
    12421 atcaattcgc ctttttcagc tcgttcttgc gcgccttcta catgatcaag aatccagcgt
    12481 atcttcgttg ctgagaaata ggcatcgatc actaaccctg ttttttcgtg gaaaaattct
    12541 tgatagcctt cattttttaa gttatctgca attccagctg actgtctgga ttgccaaaca
    12601 actgcatgat agattggacg tcctgtatct ttttcccata ttactgttgt ttcacgctgg
    12661 ttcgttatgc caatcccagc aatctgattt ggtttgatcc ctgattcgat gaacgctccg
    12721 gcaataactg actgtacaga attccagatt tcatttgcat catgttccac ccaactttct
    12781 tgcgggaaat attgcgtgaa ctctttttga gaactgccga tgtgtcttcc tttcttgtca
    12841 aagataattg ctcgtgagct agttgtacct tggtcaatcg ccatgatata cgttgattct
    12901 gtcattgtga ttccctccat tatatgcaaa cgctttcttt aactactctt atcatactac
    12961 tgttttaaaa aaagaaactg tcaaaatttt attctttttt tctttttttg acagtttttt
    13021 attatgaagc tattttttaa tcaattcttt tcttttttca gcgtctctat tcttcttttc
    13081 aatcgaataa tgtcgtaagg tgacgcaaat tctgacagat aatattcaca ctgttttttg
    13141 tttctctggc aaaatgtgat caccaaatca tattctttac cttcttcata acttccaatc
    13201 tcaataccgc cgattcccct cagttctctg atcagatatt gttgaaaagc aatacgataa
    13261 atcggcagac tttctaaatc gattccaatc actactggtt ttgcaatata aaaatcgatc
    13321 atcaaaagaa cattggcata ctggatcatc aggtaactaa gctcactatc ttcagctctt
    13381 ttcctcggca attgctgcac cgctgtttcc tgtaatttcg ccagtaactg tgttaaggaa
    13441 tgcccgagca ttttctgctg ctgttccaaa agccgatcac gctcatagac ttcgatcttt
    13501 ccaacaaaga aaaaccattc attgttgatc tgggaaattt ggtagcctac tgccttttct
    13561 tctttgattt tcaaacgacg aggacgataa tgccataaca tcgtttcccg aatgtaagta
    13621 tctaaaacag cggttggtaa ctttttgctc cgtgtcaaat catactgata aaatatttct
    13681 tcgtctacta tcgaaaaaga aacaaaaaag ctgtaaaaca tcattgtttc gtatacattc
    13741 agttctgctg ctgttcgact aagatgcatc acgatgattg gatcgattgc ttgataaagc
    13801 cggtccgact gatagagtaa tttcttttct ttcaaagttg cgtacgtact tttttcattc
    13861 aatagtcttt ttttcgtaat cccgagccag caagcaatct gtgcttctgc tgatgccgta
    13921 aacgttgttt ctaatacacg gtccaatcct agaatgaacg gtcttttttc tggtgtattt
    13981 tgcaaaaaaa gcggtcgctg atcttccggg atatattgga atagttcata ataaaaatat
    14041 cgtatctgta gctcttctcc gtataattga ccgtttttga tttgtaatcc aaattcctct
    14101 aagagctggt tcaattcttt gatctttcga aaaacagaag actcactgat attaaacgct
    14161 gtcgctaatc gaacgatgga tacttgctga tgttctaata aatagacgag catcttaaac
    14221 ttcaagcttt cctgcaataa aaacaataga attttttcca aacttacatc ttcgtccaac
    14281 acaagcagcg cctgatactc atttcgctgt acttctactt ttttttccat cattcggcca
    14341 atcaattcaa tatcttccaa atattgatca aacgaggctt ttgacagttg gacatgctct
    14401 ctcaaaccat tgatagaggc tgttccccct tgcagaacaa ggtttcgcaa aatcaggatc
    14461 tgtaactgct cttttctttc taataatgct tcaatcttca taggcagtat tcgccacctt
    14521 tgaccacacc atatctggca cacggtattc tggctggaaa aatagctcat gttcttctaa
    14581 ctgttcaaac gtccatcctt cttttactgc aattgtcagt tggtcaagaa ggtggaacat
    14641 ctcttttttg ctttggagct gtgctccaag aatctttttt tcagaatcaa atagaaattt
    14701 gagtttaact ttgctttgat ttattgctgt aagcttgtag tctacgtatc ctgtttcaat
    14761 tttttgggga taaaaaatgc tctcttcttc tgtcaaacct acgcttccta agaaatagcc
    14821 aaaccactga ttgccgactg gacgataggt tccctgatcc tttgtctgtg agccactaat
    14881 tgtcttgctg acatttcgtg ctgtacggac agcgttattt actagagaaa catacatttt
    14941 ttcatttgtt ggacgaaaag ttacttggat cgcatcgcca atcgcataaa cgtctttttg
    15001 agacgtttgc aagtagtcat ctacccagat cgttccatca tcattcaatg ttaattgttc
    15061 ttcccacatc tgattgtcgg ggcgagaatg attagccagc aaaacaagat catagttctc
    15121 ctttcttttt tccgtctcta caatagcggt ttcattttcg gtcaaagaca caacttgttc
    15181 ttctaaaaaa agtgaaagcc cttgattttt catttccttt actaacggtt ctgtcatttc
    15241 aggatctaga taacgaaaaa gaattgactt tcggctttcg taaagatgga tttgtttttt
    15301 tcttgatgcc aacccttcag ctatttcgat tcctgcttgt cctgcaccaa taattgcaat
    15361 tttctttgca ttcatgatct tttgctgaag agccgttacg ttttgtatct ttttaactgg
    15421 gtgaataagc gttgattctg cagaaatatt tcgaaacttt tgatttgaac cagtcgctag
    15481 aatcaaacga tcaaaatcca atttttttcc tcctgctaaa acgacttttt tttctttcaa
    15541 tcctattact gtcttttctg tgtaaacatg gattcgatag cggtcagtca gttcttcttt
    15601 cgtgatccaa gaaagttgtt tttcactggt aacctttcct tttaatatca aattgaaccc
    15661 gctgggaatc catcctaact tcgattgctt ttcaatcaaa tatatttcac ttgttggcat
    15721 caattggcga agataaatag ccgcatgaat ccctgcaaac gacccgccga caattacaat
    15781 gttcatctgc attcctcttt cttataaggt gttctactgc taacttctca atcagaaaac
    15841 acagtattca taactcgctt tcaaccttat gatacatagc gatgcagcag aatcaaccct
    15901 ctccatcata ccgtgttcta ggtctgcttc tcaataatgt actagtggac ctaattgaag
    15961 agtatcattt ctgaagagat gtttcaaaaa atgtactctt ataaaagaaa aaaactcgcc
    16021 ttttacgtac ctctgtccaa ataggaaaca gaaaagaacg aaaagacgag ttttagcgtg
    16081 tgtaatgtaa tagttagaat agacacatca tttattttta attctttaat gcttcaacaa
    16141 ctgcttcgcc taattgatga gcagattgag gattttgccc tgtaagaagc cgcccatcaa
    16201 tttgaaccac ttctgaaaaa gctgctccag actgatagtt tgctcctttt tctttcaaag
    16261 catcttctgc taagaatggt acttcattcg ttgtaccatt tgcttcctct tcttcattac
    16321 tgaacccagt cactgtacgt ccagctaaaa tagattttcc agactcatct gtaactggca
    16381 acaatcctac aacaccgtga caaacagctg agaccagccc ttcatttcga tagatttgtg
    16441 aagcgacttt cccaagatct attgcttctg gaaaatccca cattgttccg tggccaccag
    16501 cataataaac cgctgcataa tcatttgcat ccacatcatc aggacgtaag gtatttccca
    16561 aggcccgatt tctgaaatct tcatcttcat aataggccca atcgatttca tccatattca
    16621 aaacactttg cggttctaaa ggtacatatc cgcctttagg actgacaaaa tctgcatcga
    16681 tcccattttt tactaataca tcataaaaat gagtcagttc acttaaccac aaacctgtag
    16741 gacgctcgat cttttcgtac ttttctacat ttgttaatac gatcaatact ttcattttcc
    16801 catcctcttt ctataaaaat tgcgttattg ataacgtgct ttcaagaaat tatataatgg
    16861 gcttggcaaa taaagctctt tatatgcttc ttcaaaaggt ttcgttacaa attcatcctc
    16921 acggttttcc aataatttct ctgcccagtc aggatcgacg atcatttgct gtccaattgc
    16981 tgcaagttct gcatatttca atacctgctc tacatcttcg cgattgcgga tcccaccgac
    17041 accaactaaa gggatctttc cttgaatcgt gttatggacc acttcaagaa tcgatttatc
    17101 gttgtattta tcagaacgag ctattcgatc atacatattt aatgaaacat gcagataatc
    17161 tagttttgac tcacgtaatc gttcaagaag ccagatcgtg tcttcaaaac ggatacctgg
    17221 tgtttcatat tcttctggtg agaaacgata ccctacgatg aatggttttt tagcatattg
    17281 ttcaaccgct gagaaaaccg ttgttaataa ttcttcaata aaacgataac gattttccaa
    17341 gctaccgccc cattgatcct ctcgtctatt cgaatgcgga gagaaaaatt gttggatcaa
    17401 atacgtattt gctccatgca actcgattcc atcaaatcct gattcaatcg ctctacgtgt
    17461 ggctttgcca aatgcttgga ttgtttcaac gatttcttct tcactcattg ctcgtggtgt
    17521 ttccgcatca ggacgttttg ctgcaactgc acttgcagat acaggctgtt ctccagataa
    17581 agttgctcga cttgtcattc gtccaccgtg gaaaatctga ataatcgctt ttgcgccttc
    17641 tccttggatt gctttcgcta gctcacttaa tcgaggaatc atttcatcat gagcgatgct
    17701 gagttcacct ggccagcctt ttccgatatc ttggacattt gcagcaccag taataacagc
    17761 accaagacct ttagaacgtt gtttataata agtgatctca tcttcggtga tcactccatt
    17821 gaaaaagctg agttgtgtcg tcataggcga cagcatcaat cggttcttca attgaactcc
    17881 tgaaggtaaa gtaattgatt gatcaaattg tgacattgtc attcctccca taattcgctc
    17941 tgattaacct atcgagaatc atctatcatt aaaccactcg attagttgta catgcaacta
    18001 atttgcttaa aaaattttat tttttttgta acttatctgt tgcatcattc attgtctggg
    18061 ctaattcttt acttgcttca tttcccaaaa ctgctttgta tgactgatga agttcttccc
    18121 agctaccatc gacttcctta gcaaattggc gtccttgttc tgtaagataa actaatgaca
    18181 agcgaccttc gctaactgtt ttgacgtatc ctttatattc taatttttca acaaaacgag
    18241 caatcgttga tggggcggaa tagagcattt gtgcaatatc tttttgtggt attccatctt
    18301 tttcttttaa tatcaataaa ataaaaccat aagttggtgc cagacctgtc cttctaaacg
    18361 cttcatcagc taatttattc aacatgcgat ctagcttttt cacgctaaag aaaagacatt
    18421 catctaacgc tttttcaatt ggcttttcca tttcatcccc tccattggcg tacatatcat
    18481 agtatgattt ttattttttg tcaaaaaaca cggaataatg tccgcctcct ctgtctagat
    18541 ttgaaaaaaa gaagtagcca tcttcaaaga atttcggctt gttttcgaca aaactccttc
    18601 tggaacaccg tgaatcagag gttgtgaaaa agtcgtttag ttttgagtat gaagaaaaat
    18661 tttgtgaaaa tggctttcca catttttgca taaatttggc ttaataccga aaaactggct
    18721 tttgaacacc gtttatatca gaggttgtga aaaagccgtc ctgcatcaag taataagaac
    18781 agccaaacca aaatagttcc tcatattttg tcgttttttg agctaatgcc gatggtcagc
    18841 tgattgtaca ccgtttattg agaggtcgtg agcctgccgt tgaactccga gtcacaagga
    18901 gcaatttcaa aaaacagctt ctcatgtttt atgactatgg tgacttgtga tcgaggagtt
    18961 ggcaggagaa tactgtttat tcgtaaagaa ggggctgtga caagactttt gtcacagtcc
    19021 cttatttcct tttattatag gatttcgcca tttgattgga tgacttgtct gtaccaatca
    19081 aatgattttt tctttaaccg tttctttgtg ccattccctt cgtcatcttg atcaacatag
    19141 atatatccat aacgttttga catttgtgaa gtagaagcgc tgacgatatc aatgcagccc
    19201 cacgacgtat aaccaaataa atctactcca tcttcgatag cttccttcat ctgctcaata
    19261 tgctgcttca aatagtcaat gcggtaatcg tcttgaacgg ttccatcttc ttctactttg
    19321 tctatagctc cgagtccgtt ttcagctatg aacagtggtt tttgatacct atcatataat
    19381 tgattcaaag caattctcaa acccttagga tcaatctgcc agccccattc acttgatgga
    19441 agatacggat ttttaattcc tccagttatt aagtttcctt ggaccccttc caaattttct
    19501 ggatgagcac tgactgcact agacatgtag tagctaaaag aaacaaaatc aacagggtag
    19561 gctgctaaga tttcttcatc acctacttct atttttaagt tgacaccttt ttcttcaaac
    19621 attcgttgcg tatacgctgg ataatatcct cttgcttgaa catcactaaa gaacaaagaa
    19681 gttctttgct gctcaattgt tttttgaata tcatccggat cacaagaata cggaacaaga
    19741 agctgatagc tgatcataca accgacttgt gaacctttga taatttcatg acaagctttt
    19801 gtagctaaag cacttgctat aaactgatgg tgtgcacctt ggtatttcgc ttgttccaaa
    19861 tggggatggt tttcttcaat tactcctacg ctaacaaaag ggtgatgttt tacacagttg
    19921 atctcattga aagtcatcca atattttact ttatttttat agcgtttgaa tactgtttca
    19981 gcgaaacgaa cgtaaaactg aataagcttt cgattaggcc atccaccata ttctgtaacc
    20041 aaatataacg gcatttcata atgggaaaga gaaacgactg gctcaatccc atattttaga
    20101 cattcgtcaa acacttgatc ataaaaaaat aatcccgctt cattgggtgt ttcttcatca
    20161 ccatgaggaa aaattcttgt ccatgcgata gacatccgat agcatttaaa acccatttca
    20221 gcaaataaac ggatgtcttc tttaaagtga tgatagaaat catttccgtg tcttttagga
    20281 taataatact tgctgttggg atcctgcgcc gcttttactt gctgatgcgt catcatatgc
    20341 cattgatctg tccatttttc tttaggtaaa tcgctgtcga atgtatacac atcagacaca
    20401 gataatcctt tttcatctac atcccatgca ccttcacatt gattggccgc tgtcgctccg
    20461 ccccataaaa atccttctgg aaattttttc atatcgatcc ccctaaactt gctagttttc
    20521 ttgtttgatt atttttctta tgactagtaa tgatattttt gaatctacta ctatttgtaa
    20581 caataaccat tgtggtcgga tcatatccct ctttgattaa ttcttgcaaa tctacttcca
    20641 tcaataaatc gcctttacgt actttttttc catcagatac tgcactttga aagtatttgc
    20701 catttaaatt cactgtatca ataccgatat ggatcagtat ctctgctcct tcttcactga
    20761 ctaccccaat tgcatgtttc gttggaaaaa ctgttgaaac ttccccgttt acaggactgt
    20821 agatttttcc gtccgttgga atgatcgcaa acccttctcc taaagatcca ctagcaaatg
    20881 ttggatcgtt tacttcagat aaatcaatta tttcacctgc tgcaggagta cttaacacta
    20941 tatcctttga ggattcttct tcgatttcat ccttgtagcc gaaaagaaaa gttagcatac
    21001 aggaactgat catcgctaag aaataactag caactagcca aacaaatcct tcaccaatat
    21061 aaatcgggaa agacaaaata cttcttgtag caactgccaa agcaccggct cctgcggcac
    21121 cagctactcc accaaatata gctccacata tacaagcaat gtaaaatggt cgtttatatt
    21181 taagagtcac accataaaca gcaggttcag tgattccaaa taatgctgtg ataaatgcag
    21241 ataaagataa actttttact ttcttatttt tcgttttcaa aaatacccca agtactgctc
    21301 ctgcttgcgc agctacggca gtcatacatg ctggacctag cgtatcacga ccgtagaaag
    21361 aaagattgtt ggtcattact ggtacaagtc cccaatgtaa cccaaaaatc actaacactt
    21421 gccaaaatcc gccgataaca gcaccagata aaacagggct aaagttataa acagctaaat
    21481 atccagctgc aatcagctga cttgcataat ctgcaattgg acctacaact aataaagtaa
    21541 ttgggaccat cacgatgata caaatgaatg gtgtcaataa atttcgaata ctttcgtgca
    21601 agcttttgcg aatcttaggc tcaatataag aaagaaccca tacagctaaa attgctggaa
    21661 taaccgtact tgtatatctt gctagaatca cgggcatccc tagaaatcgc aaggttatgc
    21721 tatcactata tgcactgaca atcgtaggat aaacgagagc tgctgcaata accatagcga
    21781 tgaaacgatc tgtattgaac ttttttgcag cagtatatgc caaaaatata ggcaagaaat
    21841 agaaaaatgc atcagctgct gcattcaaaa tgatataagt tcctgattca gtagttaata
    21901 aattcaaact cgtacataag gcgagtactc ctttgatcaa accacttcct gccaagacgc
    21961 ctaaagtcgg ggcaaaaaca gcggcgatca tgtctataaa agagtttaaa atgccttttt
    22021 tctcagttcc tctctttcca ctagatgcat catcttctag tttcatttgt ttggataata
    22081 cgtcatacac ttcatttact gcatttccta ctacgacttg aaacattcct ccagcttcga
    22141 ctacagtgat cacaccttct agttgctcta ccttctcctt atctgccttc ttattatctt
    22201 ttagggtaaa gcgtaacctt gttgcacaat ggtaaacatt gctgacattt tcttcgcctc
    22261 cgacaaatcg gacaatatct ttcgctaatt gtgaatagtc catttttact tcccccttgt
    22321 taattaatgc cgtagcaatc cttcttacaa gataagtata gttcttagaa aacggtttct
    22381 ccattcaatt agtacccctt ctaatagcga acgaaactgt acaatagtgt gtatggttta
    22441 cttagccgag caaatcactc actgcttgaa agaaaacaag tatatcgttt ctcttatagt
    22501 caaaggagac aaaaaaaaca cgcttgatct tcctttgctg atgtataagt ggtatctcaa
    22561 gctgatactc tgtgattttt gtttctactt ggcttttgat aagacagcac aacaaagtct
    22621 cggcatttaa cggaagcata actaggtcag aactaggaga gattagttgc tgaatggaat
    22681 ctatcttttt atggattttt atttgatttt cttctttgta gtaatgatca agcagttttg
    22741 ttttcttttt ttcttcaact atggtcagct tttcacgcaa aatctgataa atctccgaag
    22801 attcaaactc atctagaaca ctaggtagat aaatcaaatc atcccactca aaaataagct
    22861 cttgctcagt agtcaaaatt gcatcatatt ccgttatttt gtttgattga tttttgaaaa
    22921 gatacactgg ttctatccgg cattctttga tccacatgtt catactttgt tgaatctgtt
    22981 tttttagtgt attcaaaata ctaattggct ggttactaac aatcagtatc tccccttgtt
    23041 ttcgaaaagg attcaacgct tcggcaagat acaaaaccaa ataagcgctt tcagctaaat
    23101 tgggacgtaa ttgaaaatgt attgggaaga aacgccgaat taaataaatt tctaaaggat
    23161 aacgggagat gatcttttgt acaaaatgat tcatggtatt atgtccggca tccatcctcg
    23221 ttttcatttg tttgatatgg gttagcaaaa attctgtttc atggaacaat aacgaaacag
    23281 gaagatctaa aaaacgaata atcgtttgct caaatgctct tagattcttc tctatttctt
    23341 tgtttttttc atcattaatc atcaaataat ttaactctaa tagtcgattt tcaattgctt
    23401 gtttttcttg attggaaaaa gaatagggta gttctgatga taactttttt gtgagggtct
    23461 ctaccatcgg aataaataat gtatacctgt ttgttttttc taaaagttct tctcttcgaa
    23521 gaatggtcaa aacgagatac cgagaaaatc gtatgaagtc ctctcctgaa acaatataat
    23581 tatatgatat aagaacctct tttaatgtct tttggacagt cggcatgata ttggaaaaaa
    23641 ttgtactgat ttgttgaggc aaccttgcgt gttggatcac cgtttctgtt ccaacaagag
    23701 ataagaaaat tctttttgaa ttttcttcag caatgatttt tagtcctttt gtcgacgaaa
    23761 cttccagttc taatttagga ttgcgctcga tccaacgcaa aatcgttttt atttgtaatg
    23821 atacggctgt ttttgaaaga aagaaaatgt ctgctatctt ttgcatactg acataccctt
    23881 tttgaaacaa taagtacgtc acaatagttg aagctcggaa atccatgctg gaatagattt
    23941 tatgtttcgt cacctcttgt atgatttgct gtttagtttc agattcaatt gattctaaaa
    24001 aataacctga acgtttattt gactggataa tgattttctt ctcgtagata gcaagcatat
    24061 ttttgatttc agtttggatc gttttcttgt tggtacctaa agcttcagct aattcttcgc
    24121 ttgttatcca atgattctta tttcttaata ctacaagaat ttggagttgt cgtttattta
    24181 agtccatttg cttacacctc ctctttgtta taaacataca ggaaaaacct ctcaaatgac
    24241 aatagtcact tgcagaggtt taaaaatcat ttgaagaaag atctatattc aaggttttta
    24301 ccattagact ggataatttg tttataatat tcaaaactat cttttttcaa tctcctaaag
    24361 cttccgctcc catcattttg tctatccaca taaataaagc catatcgctt cctcatttcc
    24421 cctgttcctg ccgatacgat atcgattggt ccccaccata agtaaccaat gatttggcaa
    24481 ccatctttta ttgcctcagc aatttcctga agatgtattt ttagatactc acaacgtttt
    24541 ggatcgtgga ttcgattcaa ttcatctggt gcttcatcta gtccaatgcc attttcaact
    24601 atgaataatg gtaattcata acggtctgtc aattcattca tgacataacg aaaccctaga
    24661 ggatcaattg gccaactcca aggttcggga gatttttcct tcaaaaaggg attatcgaaa
    24721 ccatttgctc cgccagtatc tacgcgcatt tctgcttctt ttttatagac agcgctacga
    24781 taataactaa atgcaatata atctaccgtg ttagcttgca agtactgttc gtctccatct
    24841 aacattactg gtgcacagtc ttcttccttc catatcctgt aaatataagc tggataatgc
    24901 cctcggcaca tgacatcgga gaaaaaccat gaattgcgtt taaattgcag tgaaccaaat
    24961 acattctctg gatcacagtc atatgcataa gtcgctaatg cagaaaagct acacataata
    25021 cccatcttag catttggatc gattttcttt aataattgag tagcacgggc gttcgctaga
    25081 aacatatgat gacaggcttg ataaatctct ttttttgtta gtgtagcaat gggttcaagt
    25141 ttatctctag cagggaatgt aaccaatcca gcagctgcat aaggcatacg aaaagcatta
    25201 ttgatctcat taaaggtcat ccaatatttc actttccctt ggtagcgttc aaagacggtc
    25261 tgcgcataaa attcaaaaaa gtcgattaat tttcgatttt gccacccccc atactctcct
    25321 accaatgcta agggggtttc ataatgacta agagtaatta ctggctcgat atcatatcga
    25381 tttaatgtat tgattagtct gtcataaaat acgagaccag cctcatttgg cttctgttcg
    25441 tctcctcttg gaaatattcg tgcccatgag atgcttgttc gaaaagcttt taaacctaac
    25501 tccttcagta aacgaatatc ctcttcaaat cgatgataaa aatcaatcgc ttcatgagaa
    25561 agataatgga gtgattcgtc taaagcaatc tcccaaatct ttttttcttc attccactga
    25621 atactaggat gctggttcat gatcccgata agcgtatctg tcacattagg taatttgccg
    25681 tcttccaacc atgcaccttc acattgatta gcagctactg ctccgcccca taaaaaattt
    25741 tctggaaata tttcctgatc cataactatc tctcctcgct atcaacaatt tcttacttga
    25801 agtatagctc gattcatcaa aacaagatat tctaactatt tcggcagaag agaagaaaaa
    25861 aattgaatgc tactcttcat ttttaatatt tatatataac aaatacataa gacgatcccc
    25921 gaaagaaaac actcgttaac catctaagca aacgatttat tttacttcca acacaaatgc
    25981 ttcataaggt cgtaaatcaa ctttgccgtc tggtaactgt ttcagaggat aatttgacac
    26041 aataaccctt aaagcttcac gtggaacagt gaacgtctgc ttttcactgg acatattaca
    26101 aataacgagc cattgctggt cttcatatat gcgagaatag gcaaagacat gctcaggcgt
    26161 cttttccact aattcatagt ctccccagac gatgatgtca tgttttctac gcaaatcgat
    26221 caatttcttg tacatataaa aaacagaatc ttgatctgct aacgcttctt ctgcattgat
    26281 ttctttataa tttggattga ctcgaagcca tggaattcca gtagtaaacc cagcatgttc
    26341 acgggcgttc cattgcatag gcgtcctagc attgtctctt cctttcgcat tgattgattc
    26401 caagatttcc tcttttgtaa atccagaaga aatcctttcg ttatacatat tgattgtctc
    26461 aatatctttt gcttccgcga tatcatcgat tgggcaattc gtcatgccta actcttctcc
    26521 ttgatagata taaggggtgc ctttcatcat atggagcaag atagcaaaca tcttggcact
    26581 ttcttttcga tacacatcgt catttcccca acgagaaaca attcgcggaa gatcgtgatt
    26641 attccaaaat aaactattcc atccttcatc acctaaagat gtttgccatt tggataatac
    26701 ttttttcagt tcagagatac tcaagggttt caaatcccat ttgtctttgc cttcttgctg
    26761 atctaatcct atgtgttcga attggaagac catggataat tcatttcttt taggattgga
    26821 ataaagttta gcgatttcgg tagttgctcc ccatgtttcg ccaacagtca tcagattttt
    26881 gtcaccaaaa gttgcttgat tcatttcctg aagatactca tgcagtttag gtccattact
    26941 tgtgatttct ttatccggtt ctttgccaat catatcgatc acgtccatac gaaagccacc
    27001 aataccttta tcgatccaga aattcatcat ttgataaatc tcttggcgaa cctgtttatt
    27061 tttccaattc aaatcaggtt gacgcttgct atacaagtgc agaaaatatt gccccgtttt
    27121 ttcatccaac tgccaagcag aaccactgaa tattgagcgc aatctattag gctcttttcc
    27181 attgaccgga tcacgccaaa tataatagtc ccgataagga ttttctttat ttttacgggc
    27241 ctcaatgaac cacggatgtt catcagaagt atgattcacc accagatcca tgatgatacg
    27301 gatatttctt ttctttgctt cttcaatcag ttcttccatt tcttccatcg taccgaattc
    27361 tgccatgatt gcttgataat tactgatatc atacccattg tcatcattcg gagactcgta
    27421 tactggagaa agccaaattg catcaatacc taaatacgcc agataatcca acctggaaat
    27481 aatcccccgc aaatctccaa taccatcttg atttgaatct tgaaaacttc ttggatagat
    27541 ttgataaaca actgaatggt gccaccattt ttgttccatg attttcctcc tataaatgga
    27601 tgtttctatt ttttgcttaa tcatttattt tagtctctct cactaaaaat tgtaacgctt
    27661 tcaaatagct attgcacctt gttatcggta acagaataaa acaaaaaaac agctgaggaa
    27721 attccctcaa ctgttttttg aaaggattga tttattcagg gaagaatgct gatttcacaa
    27781 cttctttata aacggtgttc actcagctgt ccctcggtat tagctcaaaa aacggcaaaa
    27841 tataagaaac tatttttttc gtttttttct ctaatactca ggccagaaca ctgatttcac
    27901 aacctcttta taaacggtgt tcactcagct gtcccttggt attagctcaa aaaaaggcaa
    27961 aatatgagga actatttttt cgtttttttc tctaatactc aggccagaac actgatttca
    28021 caacctcttt atttcacatt caatatccac atgaatccca tgaatacaaa gaacagacca
    28081 tacatgatgg gagatacttc ttttcctctt ttagctgcta acatggtaat tggatagaag
    28141 ataaaaccta atgcaatccc atctgaaata ctataagtca aaggcatgcc gatcaagatc
    28201 aagaatgaag ggatcgcaat ctctaagttt ccccattcga tttgtcgcaa agattgtgcc
    28261 atcaatactc ctaccataat caatgctggt gcagttactt gtgaagtaac aactgatagt
    28321 aatggagaaa agaacaagcc taacaagaag aaaatccctg tagtaatagc tgtgatacct
    28381 gaacgtcctc caacggcgat accagctgat gattctacaa atgcaccaac aggagaagta
    28441 cctaataagg aacccgcaag catagctgtt gaatcagaag ctaaagcttt ccctacacga
    28501 ggcatcttgt tgtctttcat gaaacctgct tggtttgcta atccaactaa agttcctgct
    28561 gtatcaaaga aagtcacaag caagaatgtc agcactacga cccacatttg aagagaattg
    28621 atgtccccta catgttttaa tgcaactaaa aatgttggtt tcaaacttgg tgctgcggca
    28681 acaatttcag aaggcattgg aatcaaacct gtgaccagac ctaaaatcgt tgtagctgcc
    28741 atcccgatga agatgccacc tggtacacgc cgaacaagca agatagcagt tacgactaat
    28801 ccaaaaatcg tcaaccaagt agaaccaacg ttgagtgatc ctaatcctac taatgtagat
    28861 tcattcgaaa cgatgatccc accttcgctc agaccaagga aagcaataaa tagaccgata
    28921 ccgccagaaa tcgcatattt caaatctgct gggatcgcat caatgatcat ttcccgcagc
    28981 ttgaagattg tgatcaaaat aaaaatcaaa gaagcaacga atacgccggc taatgctgtt
    29041 tcccatggaa tccccattcc taaacaaaca gaataagcaa aaaaagcatt gatcccaaga
    29101 gcaggtgccg ttccaattgg ataacgggct aataccccca ttaaaataca gccgatcgca
    29161 ctggctaatg cagtcgcagt aaacactgct ccttcatcca ttccagaagc acctaataca
    29221 gttggattta caaataagat ataagccatt gagataaatg ttgtaaatcc tgccagtacc
    29281 tcacgtttta ccgtggtgtt caatttttct atttcgaagt aggaaagtat tttttccttc
    29341 atcttccatc cccctaaatc cgaacattat aaataaagtc cgttaaaatt tcgatttatt
    29401 aataaaacaa cgtgttttat catatactaa aacaaaacat aagtaaacaa aaaattcgga
    29461 ttttaagcaa aatccgaacg ttccatctga ttaaatccct ttatctcaaa gttcatgaac
    29521 attcactttg gattacaaga atttctttat tgtgttaata aagtttcttc attctagtct
    29581 caaaaatcaa tagtgtgatt tgattttacc atttcgtttc tgttcttctt tgatgaattc
    29641 ttttgtatcg acaggattca gattatttag tccgccaaaa cctgtatcga ccatttttcc
    29701 ccagcctttt gtcttctttt ttcctgattt gagattcttt gctcgaagct tctcactatg
    29761 ttttgctcgc cgatacttca aataaccttc taatcttccc attagatcca ctgtttctcc
    29821 tcctttttat tcaatcagac gactcagctt ttcctataac aaaataattg ccttcagggt
    29881 ccgaaaaatt gaaagtcaag acgccattgt tgtctgtgat ttctcctggt tcttccatgt
    29941 tctcgtagat agtttcaaat tcatccgtaa aaaatactaa agaaggcggt ggtcctaata
    30001 cctctggtga aaacttctgc acaaatactt ttgggaatac gcctaattct aattcttttg
    30061 agatgctgag tacaatactc ttaaattctt ctggcaattc gattgtttca gaaatttctg
    30121 caccaatttt ttctacaaag aatcggcaaa tcatctctac gtcattgaca taaagcatca
    30181 cacgtgtttt ttgtatcatc gtttgaccct tctttctatt tgattttctt tcctgtctat
    30241 aaactgattc cattatatca cgaaagcgca aacaccttgt gtttgatact attcaagaaa
    30301 aataaagcaa tagtttctag gattgctttc ctccacaaac tttttttaca aaaaaaaaac
    30361 ggcaatgaat gccgctcttc ttatttcgat tcttttataa ttctgcttct gattcatctt
    30421 tggttagtgt gtgtctaaca ctagttacgc caaatgtttg atcgattttt tccttcaacg
    30481 cttcgattgc ctgtgcgcca cgtgccgctt gttcagcatt gatggattca atgaccagca
    30541 ccgctgcttg tgtttcttcc gtcagcatcg ttctttgggc ttcacgccag ttgagacagc
    30601 gacagatcgc tccttgttca tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg
    30661 aatctgcacc taaaggcagg aatgattcgt caccttgggc ttttcctaaa tgcagatttc
    30721 ctgcgatctt ttcgatatct tctccgccgc aaggtacgcc aaaagacaag gaaatactat
    30781 tatagatatc tacaagcgga ttgattggga aaaattcccg ttcttgattt gcacgtttca
    30841 gtaaagcttc aatagaagaa cgtgcacctt ttttagtctt gaatgtggta aatgcctgac
    30901 gccactgagc aatgacttcg ttttggctga atgtttcttc tgtaaggtaa tttcttgctt
    30961 cttttgctgc ctcattcaat aattgatgat acgtttcttc actttctttt gttacgtggt
    31021 tatcgatacc atttacggat aagatttcga tggttgcttc aggaaatact tcccagaaat
    31081 tggtatccac tattactttt ttcatctgtt cacacctctt gattagaata attgcttgat
    31141 gaatagatag tctgtttggg gatcatctcc catataaaaa acatgttcgc tgtatttgat
    31201 aaatcctttt ttacgataaa aagcgagcgc aacatcgttg tgttcccaaa caccgagcca
    31261 aatggctgtt ttttgctttt cttttgcttt ttcaatagct gtatctagta aaaatgtacc
    31321 gataccttga tgtttaaatt tttttcgtac atagattcgc tcgatttcta aaaaatgatc
    31381 agccatttcc tcagattgac tcgttcctgt atttagtttc aggtaacccg caggctcttg
    31441 atcgatactc actaggaaaa aatctgtttc tttatctttt agctcttctg taagtttttc
    31501 tagtgcataa gcttgtgtca agtaatcagc taaatgctcc ggtaattatc ttttccaaat
    31561 gtatctgtaa atgtttcgat actaatttgt tgtagtaagt ctacatctga aggcgtcatt
    31621 tttcgaattt cgtatttatt cacggcttct tcctttcaat agatcctttt attgcctttt
    31681 ttgacaaaat tccaatcttc gctaacattc ttgcatactt tatttaaaag atattccaat
    31741 tgttttgctt cttcgtcaga aagaccattt aatgcgactg cattggagta gttgttttct
    31801 cgaatgatga aaggatagat ttctgctcct tttttagtcg gatagagttt cttgattttt
    31861 ttattttctt tatcttccaa acgttcgatc atcccattgg attctaattt cttgattgct
    31921 cgggcagtcg ttgtacgatc tactttgatc atttctgcta gtttttcttg gatgattcca
    31981 ggattttcgc aaatacgtac taaataaaga tactgtcctc ttgttaaatc gacttctttg
    32041 aattcgatat tgctaataga gtctagcgct cgggcaatca ttccgatgtc tcgaaggatt
    32101 tcagccattt catcccacct ttattttgtt tgtaagacta gtttacctct ttttgttgca
    32161 aatgcaacta tttttaataa aaaaatcaag ttctttttta ataactagta aagtatattc
    32221 ttttcttctt ttacgaaaaa gctcaatcac aatagatctg gtttttgttt tctaaaataa
    32281 gtaactgttt cttcgttgtt cgcttttctg atagctttgg caactcttct atactaaaaa
    32341 atcccatgtt ggaagtttca ttattctcaa taaacttggc ctcttcaccg tggatcgcac
    32401 aagcaaaaac aagtttataa tactgaaaaa gctgtggaat atctttttgt ttattcgtat
    32461 caaaaactgc tcgcaactga gtagtttcta ctgttaatcc tgtttcttca tagacttctt
    32521 tacgcacatt ttcttcagga gacaatccta tttcagcaaa tccaccagga agtgaccatt
    32581 ctttcgtccg tttatcttcg attagtagga ccttgccatc ttttttgata taggcacgaa
    32641 catctacttt tggtgttgga tgtccttctc caatcgttgt gagcgctttt atttcttcga
    32701 aagtttcttt tcctatttca ctgatcagtt ccaatgaaat cgtccggatc tcctcaaaac
    32761 gctctttatc aaatacatct tttccataat atagaccagc ttgagaaagt gcgagtagtc
    32821 ttttgtattg agccaacttc ttgttcattc tcttcccttc ttttttagca aatgtatgac
    32881 tatgtaatca agtatagaag actggtttaa ttacggcaaa taaatataat gaaatctgca
    32941 ataaataaaa gaatgttaaa aaactcgaga acaaaagaca ttcgacttct ctggggctgt
    33001 gaataaatgg cgttcaaaag cttgcatctg cggtaataag ccccatcaaa cgaaaaatat
    33061 gacgagctat tcttgtctgg ctgttcttat taattgaggc aggacagctt tttcacaacc
    33121 tccccactaa acggtgttct cctaccaact cctcggtcac aagtcacaat attcatcaaa
    33181 catgagaagc tgtttctttg aaattgctcc ttgtgactcg gagctgaacg acaggctcac
    33241 gacctctcgg taaacggtgt tcaaaagcca atttttcgat attaagccat ttctttgtta
    33301 cttattgtca ttgatttctt tttctaattt atccaatagt ggagtgtaat ccacttgagt
    33361 atagtctgtt acatcaaatt catatagtgt tcctaactgg aatttgtcat attttcggtc
    33421 ggcgatgatc tttttgaatg cttctttcgt aatatgattg gtccctttcg ttcgatcttc
    33481 caatacgtca ccatagtgat aatgatccat gatatagctc aaatgacgat ctgattcacg
    33541 atcacgtttg taccgacgtt gccataacac ttcaaagtct gctgttaggc gaatcgtgat
    33601 cacctgatat ccgtatctat ctgctaattc ttggagtcgt gctttttgct tatcactaaa
    33661 aggatactcg gacacaatca ctttctttcc tgcttccata tacaaatcaa gtgctttgta
    33721 atagaaagga taaactttcg tcctttcttg ttccgcccgt tcttccaaat tgttaaaccc
    33781 gatggattct gcatagtatt ccttgatttc atcgagcgtg atggcataca tatctggaaa
    33841 tcgttcccgt agtttattca taagatatgt ttttcctgta ccaggactac cagccagaag
    33901 aatgaaatac tttttcatct catttatttc ctaagttaat tggactttcc aagttcataa
    33961 acagtccaat cttatgcttg acaatttctt ttactgcttt atttgctgga atcacattat
    34021 ggcgaagaga cttgttgatt tctgtttcac taaaggtttc tttagctact ttcgtccagt
    34081 tgactaaaag ctccgttcca acattgaatt tgcggatccc gttattgatc aattcaggat
    34141 aatcttcttc tttcacacct gttcctccat ggataactag ggggacatca actactgcat
    34201 cgatttcttt cagcaaggga atgtttactt ctgtttttga tttgaattgt ccatgattcg
    34261 taccgatagc aatagctaat gcatccacgc ctgtagcttc tacaaatttc actgcgtctt
    34321 ccggtcttgt atataccttg tcattttcag cgacatggat tccttcttct gtaccgccga
    34381 ttgttccaag ttctccttct acggaaacac cgtgcgcatg ggcatattct actactgctt
    34441 ttgtcttcaa gatattttct ttgaaaggta gatgggaccc gtcatacatg acagaagtat
    34501 atccgctatc gattgcttct ttgatatcat caaagtctcg ggcgtggtct aagtgtaaga
    34561 ctgcatcaac catttcttct tctgcaatag ctgtcattac attgactaac acatgcatgc
    34621 cgatatattt agcggtatcg acacttgttt gaataatgat tggcgaaccc atttcttttg
    34681 ctgcgcctag catggcagga agcatttcta ggttatgcgt attaaaagct ccaactgtaa
    34741 aattcaactc ttctgctgtt tttgttactt ctttcaatgt tgtatacatc ttttattgcc
    34801 accttatctt tttatttgtt tcctttgatc gttacttgtt ttgcaatttg tcgcatctca
    34861 tccattttgc ccatgtagta atcgatccct tgatcatcac cagcacgagc agcatctgca
    34921 cgcttctggt tatagaggct catcagtttt ttatacccat ctccgatcgt ctgtttattt
    34981 tccaagctct tttctaaata ttggatggct tgatcgttct catgcaattc agaaaattta
    35041 agtccaactt gttcataaag ttccgctaat ttatgcctat cttgggtagt ggaaatctct
    35101 tgtttcaatt gtgtaatctc tgattgaatc ttttcggtcg cttctggcga tagggctttt
    35161 tcttcttttg ccagaacttt tttcggttct ttttcttttt tcttaaaaaa tccaaacatc
    35221 attttcccta ctttcaatta aaacgctttc aaaactgggc ttaagatcca ggcatataag
    35281 agtgctgcgg ctactgtatc tacatcggtt gctgtagcat tgatgaatcc catcgaatta
    35341 aagatcgtta ctagcaaagc gggcaataat gtaataaaga aaccatgagc aataccgcca
    35401 atgatcgctc ctcggcgtcc gccaactgcg ttgccaaata tccctgctgt ccctccggca
    35461 aagaaattgg ttagcatacc aggtaaaatc atcgctaagc cgaacatggg caaagtaaac
    35521 atggcaataa tcgtaccaat cgttgtcgta atgaaaccta aaatgacggc atttgggcta
    35581 taaggaaaga aaactggaca atctaaggca gggatcgcat caggaactaa tttcatagct
    35641 atccctcgaa atgccggcac gatttcacct aacagtagac gtacaccagc tagcaatata
    35701 tacacaccca ctacgaattg gatggcttgt aaaaaggcaa acatgatata gttttgatct
    35761 cctgataatt cagaagcgaa tggttcgcct gcaaataaaa cagtgatgat atatagcggg
    35821 accatcacga ccatgacaga caaataagta tcttgcagaa attcgaaatg tgtcggcaac
    35881 ttgatatctt cgatcgactt tttattttcg cctttctcac caaaaagctt agcaacaccg
    35941 gcttcaaata agtacccgat cgtacaaaaa tgacctaaag caatatcatt tgatccagtg
    36001 attttacgaa tgatcggttg cgctactgcc ggcatagcaa tggcgaaaca agctccgata
    36061 aaaccaccga ccaagatcaa tacgatccct cgcagaccgg caaaatagcc aaagactgtc
    36121 gtcatcgttg ccatccataa aattgcttga ccagttaaaa aaatgtactt ccattttgtg
    36181 aaacgagcca aaataatatt gaatacaaaa atggctaaga acgtcagtgc aatatctcta
    36241 cctaaaccca aatcattcat tgcttgatca ttgatagatt caatagaagg gatgatccct
    36301 tgcatatgaa atccttcagt aaagattttc ccgaaataag ttaaacttcc aacgatgatg
    36361 ctcgaaccgg cactcaatac ttgaaatcct aggagtgttt ttaacgtgcc agagatgact
    36421 tgtcctgctg atttcttctg cagtagtaga cccaacatag caatcaatgc aatggtgatc
    36481 gatgcctgag tcaaaatatt ttcgatgata aaattgataa cgctcatgtt tttaccctct
    36541 tttctttttg tatttagata actcccttat cttgcagaac tggggtgatt ttttcttcaa
    36601 tttcattttt tgaaacaata tttttcaaat agatcatcgc cgttttagga tcaactgcga
    36661 acttttcgaa ctgcgaacga aaattttctg ctgtgatgat cacatctggt ttcatggaaa
    36721 ctgcggaaga aatatcacaa tggtccagtt tggcttctac tcctaatttg ttcaaaacat
    36781 cctcagcaga catctgtgct gcaaaactac ttcctaaacc tgcgccgcat acgaataaaa
    36841 tgttcaattt gttcatcttt gttctccccc gtatcattta ttatttctca actactgaaa
    36901 aaagcagttg tttgaaatct tcgatatttg tgcattcaat caatcgatcc aacttctctt
    36961 cgttgttgat caattcaatt agttctttca tgatgttcag atgggagtag gagtctactg
    37021 ctgccaaaca aaaaataatc tttactggat ccatttcagg attaccgaaa tcgattggtt
    37081 gctcaatcgt agctacgctg actcctaatt tattgacccc atcttctggt cgggcatggg
    37141 ctagtgccat gtgtttgcca atcacgatat acggtccgta atcattcact gcacgaatca
    37201 tcgcttcgac atagcgctct tcgacaatgt tttctttaac caatggtcta gcagctagtt
    37261 caatcgattc ctcccaagta gtggcttttt ctttgatcat gatattgctg tccgtcaaaa
    37321 tatcttttaa cataggctgg atctcccttt tgttgatttc taaattgttt gtttcgaata
    37381 atttttctaa cttgccatag atgattcgat ctactcggcc accactttct ttcatgacat
    37441 ctattatttg agtgaataac tccgtcgatt catgactgtt aggctctaac cgctgtagtt
    37501 cttcatttct tttcaaaaag tcttctataa ccaagatatt cgtctcagtt aaaatcggat
    37561 cgacaactaa aagcggctta tttcgataat tcaattgaaa ggtgctgaaa accagattga
    37621 catccaattt atctactaat tcgatttctt ttgaacttaa aactgcagta atctcgatct
    37681 gaggaaactt ttctttcaga ttctctgcta acagatttcc agtagccatc ccatggttac
    37741 agacaacgac tgatttataa acataattca agtctcgctt gattacactg gcgatcgtcg
    37801 aaaagtggat gacaagaaaa gctatctcgt cctccagtat cttgccacca attacttctt
    37861 cgatcgtagg aacaaaatga gtgactgcgc ggtagattgg gaaataattt gttcggatgt
    37921 tttctttcaa tggattcatg acttggatat gattcttgat tcttacaatc agtgcagcca
    37981 tgtgcttata gagattttcg aataaggctt cttcctttag atggaaaggt acgtgtgtct
    38041 cgttctctac aaattggatc aattggatcg ttagtaattg agcttgtacc cactcgattg
    38101 aattggagat atctctagca ttgaacgtct caatagtgaa acgaagataa tttctttcta
    38161 cttgcgggac attgtccaat tgaaattcct cgatcatgtg gtcaataaat gcagaaaagc
    38221 tactgtcttt tatttctgta tttttccaat taatggcaga gataaattca tgtttcctga
    38281 ttcgctggat ccagatcaat gtaaaaagca ggatttgttt tttataaata tcatcttctt
    38341 atttcaacaa cttattggaa tagatctcct gtattttttt gatttcctcc aaaggaaaat
    38401 aatggagaaa gattttttga gccggtgtta attgtgcatc ttgattcata aagaagtcgg
    38461 tcattcccag attctgattg ataaaatcat agatcatcgt acggatagat cgttctgaac
    38521 ctttataaac cagtccttgc tttccataac tgacgatctc tatcccataa ctgaccagtg
    38581 ttgctcgtaa ccgtctcata tcttcatcta atgtactttt agagatttga aattcttctt
    38641 cttttttgtt tagatggatc gggattcggc tgtaagcaac ggctaaaact agatcaaaaa
    38701 tacgttcgcg ccgattcaaa aagccgtctt tcacatcttt tatttcagtt tctaatcgag
    38761 cttgcttttc cttttccagc ttcaacaaaa aacctttgtt gcgaattgta cggatctccg
    38821 gaagcttttt actgctcaac cagatattcg cttcaagaac ttcctgacga atcgtccgct
    38881 gactgacatt gaactcactt gctagatgtt ttgcttttac tggatattgg gctgtcatga
    38941 ttctgtgaag tatttcaatt gttcttgttt tcatagaaat gatctccttc tgatttcaag
    39001 tatatggttc cgcttacata ataaaaagta gatatttctg ccacttgaag tggaaagaaa
    39061 gtaagcaaag caaacaaaaa ataagccata tgctttttca tctcctcaac gaaaaagaca
    39121 tacgcctatt attacaaaaa ttttgcccat ctatacaaaa aagaggttgt gaaaaagctg
    39181 tcctgcatca agtaataaga acaactaaac taaaatagtg cttcctattt tttgtttggt
    39241 tgttcttatt atcgcagatg caagctttta aacaccattt agtggagaag ctgtgacaga
    39301 gatgttcagc cttgagcgat aagaagcgat tttgaaaaat cgcttcccat attttacaaa
    39361 atcgtgcctt attgccgata ggctatctct ggaacaccgt ttattcagaa aaaggagctg
    39421 cgacaaggct tttgtcacaa ctccgttcat aaatatttcc tgtcaatcca gcataatcgg
    39481 ttgaataaaa tcggcttgcc atcttttttc tttctcagaa aaatcaaaat accatatctg
    39541 agaaaaatac tgtcgttgtg tcttacttcc tgagatcaat cgcccatctt ttcgatcgac
    39601 tgaataatca cagcatctga aatcaattcg gatggcaaaa ctattttcgt ggatctgtcg
    39661 ataatttcct acttcctgaa tagttatttt tttagtatga tttcttacac cgtctgcttt
    39721 gtttttttgc agaactttct gatgttcggc aaacagcttg tctgtataac agcttctggc
    39781 tttttcagga gtttcttctt cccaagcgga ttggatcttc atgaatatct tttggatttc
    39841 caaaaactgt gctttctttt ctttagccgt tcctgacatg cgggacaata tctccgattt
    39901 cagccttttc ttttgtcttt tattcttgac gatagaaaaa gcagaaaccc ctcctactga
    39961 caaaacagct aatgcaagaa accccgtttg ctcataacta gaaggacctg tacttggacc
    40021 ataataccca taagaatctc tgccgtaaga acgactgcca aaataactgc ccccatatga
    40081 acgaccgcca aatgaacctc tgcccgaact tcgaccgcta gatgtacctc caccatgacc
    40141 acccgcgaca gcgaatcctt gggtcggcag agcaaacaaa cataggccca ctaacaaaat
    40201 gattgctaaa taattgattt tttcattgtc ttcctcgtta tcttttatta caaaaaacta
    40261 tttttatcat agccttgcta aaaggaagag tcaataacca gatgcataaa ctgttttttt
    40321 agcaattttg tcttatttag tcattgagta acatcggaca gatgtatgtg tcggcagtcc
    40381 ataattcctc tatcttcagc atgaacaagc ttgcagacaa gagatctgcg cttcctccag
    40441 gagagatttg gttttcctga caaaagtgca acagtgctgt aaatggacga tttggtaaag
    40501 cactttgact agcagcatga gcaagttttt gcatttccac taacgttttc tgatcactgc
    40561 gatgcaatac gcatgtgtca tctagcgaac tcatcaaaac taacaacata tgcatttgtt
    40621 tctgtacata tgaatcatta tctagctcct tagctgcttg gattgcaagc ctgatatgcg
    40681 gataccctaa tatcgcttct tcatatgcac catttacgcc atatttttgc ttcaccctat
    40741 gaccatgagt ggttttttta tgacgatatt ttgtatcagg aaaagcagca agcttgctga
    40801 catcatcaag tattttcgat aaagaaaaag gctgttccat actgattttt tgtgcaatga
    40861 cactgatcaa tagccccatt gcccaaatcg ctcctttatg ggtattgatg ccatttgtag
    40921 caatcagcat tgccttttcc gcttttcgcc caatctcagc aattctttct cttaactcct
    40981 gatcaatagc ataatgccat gctatttgag ccatttcttc aaaaaaagga gttagcgtct
    41041 tagcagactt atccaaaatg cgccgacctt cttttacggc ttttgcgatc gtatggaaat
    41101 tatcatctcc taaaaccata tcagctgcct gtttgcttac ttcactacct ttgattccca
    41161 tagcgattcc tatatcggct cttttcaaag caggtgcatc atttacgccg tctccggtca
    41221 ttccgacgat ttctccattt ttctgcaaag ctttcacgat tcgcagtttg tgttctggtg
    41281 tcgttcgagc aaagacgtcg acttttggta cttgtcgaat caactcttct tcagacataa
    41341 gatccaaatc gatcccttct aaaactttag cagtatgttt taagccaatt tgttccgcga
    41401 tcgcttttgc tgtatctttg tgatcccccg taatcatttt cactgagatt cctgcttgtt
    41461 ggcattcctt tacagctttg actgcacttt cttttggcgg atcaatcaac ccagcaatcc
    41521 ctgcaaaagt taattgggtt aaagactcat gcgtgagttc ctgtttcgaa tctacttttt
    41581 tgtaagcaaa tcctaaaaca cgctgacctt ttttcgcaaa cgctgtggct gtttgacccc
    41641 atgcttctta ttctgaagaa gaaagagttg atttagccaa taatacttct ggcgcacctt
    41701 tgacgtaaat cacccctcct ttttcctccg catgcatcgt tgccatatat ttgtattcag
    41761 agctgaacgg aatttttgct tctatttttt tcaaaggcag atcggctgtt tcggtgtggc
    41821 gaatcagtgc aagttctgtc ggattgccac ttaaattttc tgttttctgt tcttctttcg
    41881 tctttaattc ctgacaattt ttcataataa acagtacgcg agtttcttcc tttgctgcta
    41941 catccacaac tgtcatttca ttttttgtca atgtccctgt cttatctgaa cagatcactg
    42001 tcatagaacc taatgtttct acagaaggca gtcctttgat gatcgccttt tcttttgcca
    42061 tttcatgtac tccgacagat aggatcatgg tcaacacagc tggcaatcct tcgggtacca
    42121 ttgacaccac caaagcgatc atagctgaaa gaaggatatg ccaatccatg ccatagcgga
    42181 atgttgtaaa aacaactaag aacaagatca aacaaagaat gccttgaaaa atttgtttgt
    42241 tcaatcggtt gatcttcttg atcagaggcg tcgtttgttg ttcgaccgat tgcagtgctt
    42301 gattgatttt accgatttct gtcgtgtttc ctgtttctac aacgacaccg actgcagaac
    42361 cagattgtac aagcgttcct gaaaaagcca tatttttttg gtcaccgatt gattcttctg
    42421 ctgccaaaac ggaatgattt ttttcagatg cttctgattc tcccgtcaaa atcgattctt
    42481 ctactagtag atcatgtact tcgatcaacc gaacatctgc tggaacgacg tctccggcac
    42541 gtaagacaat gacatctcct tttactagtt ctttcgcagg gattttttct tgcatcccgt
    42601 tcgatagtac tatcgcttct tgtcccatca gctgcttcaa accattcaac gattcttctg
    42661 cttttctttc ttgccaataa ctaacgagtc cgttgacgat cactacgaat aaaataatac
    42721 ttccttcgat gtattcgctc gaagcaaatt tcaaaaaaga ggcaatgatc aacacgatca
    42781 tcagcagatc agtaaagtgt tttgctattt ttttccatcg ttttgtctgc tgtcttgctt
    42841 ctatctcatt atatccttga tcagtcaggc gtttctgccg ttcttcttgc gacaagcctt
    42901 ctgttgatgt ttgatagttt ttcaatacat cttctaccgt caatctgtac cagttcatcc
    42961 tatcccctcc tgtttaattg atactgttat tatacgcagg gaagtttttt caggcgtatc
    43021 gattatttca ggggggtctg aaataattcg ttttgcagaa tgtatctggg cctaactgat
    43081 atttctattg cttattcttt tatatacaaa atttaagaat acaaaccaga taaccaataa
    43141 aaaaggaata aagcgtgtcg tgctttcgaa taacaagctg aaagcaattg aagtaaaaaa
    43201 caccaacaaa gcaagatctt tatatctttg tgtagtgttc cgtttctttt ctttaataaa
    43261 ttgaacatgt gcacacaaga tcgaagacca cacaaataga aaacaactcg ttgtcatgcc
    43321 tgccaaaagc tgaaaaaaca atcgactatc agctacaaaa aaatgtagac aaagaccgac
    43381 aaaaaataac atccctgtaa ttaaaatacc gtgtaaaggt acgcctttct tagaaagcgc
    43441 acaaaaccaa tggggcaaac ttttctcttt tgctgtttga tataaaatcc tgctagtact
    43501 gtagactgca ctatttgctg aggacaaaga agcaaccaaa atcattagat tgacaattgc
    43561 tgctccgctt tggcaatgga tcacttgcag catagtcact aaaggacttt cttttaaatc
    43621 taaacaatgc catggaacaa caaataaaat cgacaaaatc gttcctatat agaaaaagag
    43681 gatacgccaa ggcagacagt tgattgcatt tttcaaattc ttttgtgggt cactcgtctc
    43741 accaactgtt aatccgatca tttcaattcc agcaaaactg aataaaacca taggaaatga
    43801 agcaatcatt ccagcaaagc caaacgggac taattcattg agctgtccaa aatttgcttt
    43861 gattccaata gactgggtat gaaaatctga gacactcaat cctataccca ataagataaa
    43921 aaggaaaatc cccaagatct tgatgattga gaaaaaagat tccaattcac caaaccactt
    43981 gactgcaaca aggttgactg ccattaagat cattagtgcg ctacctccgc ttatcaaagc
    44041 tggtacatgt acaaaccaga aatggatata caatcctatt gctgttaatt ccgcaagtcc
    44101 agctccaagc cagcaaagcc aataagtcca attgacaaaa aatgcccact ttttgcctaa
    44161 ataatagtca acgaaatcac tgatagagtt aaatgtacta tttgctaaaa ataaatcacc
    44221 catgacccgc atcaaaccat aaatagccaa accgcatatc aaataaacga gaataacagc
    44281 tggccctgct gatgcaattg cctttgctga acctaaaaat aatcctgttc caattgcgcc
    44341 acctaatgcg attaactgga tatttcgatt agttagagtc cgtttcattg ccggtttttc
    44401 catcaagttc acctttttct ttttcaagca gcatggcgac ttcctgttcc caagaaggtt
    44461 tctttatttt ttcaatacag agtggtacta caaacaaaat gacactgatg gctgtgactg
    44521 caattagatt gatccatgtc ccagctttca tcgtcagtcc gacagaaaat ccgattgccg
    44581 caaataaaac aataacgaca gtccaaactc caagattgct ctttttctct ttttttccaa
    44641 ttctaaatgg tcgattcaac tttgcatgtg tataacgcaa tctcaaaact gcgatcccca
    44701 tgatgacata gacgatacag tagataacag tagttgaatt tacgagcatc aaaaatgctt
    44761 gattgacgcc aggaatcagc aaataaaaaa gtgcaaagat tgagataatg acagactgag
    44821 tcaaaataac ggatttggat acgtcaaatt gattcgtctt ccaaaaattg aattttggtg
    44881 gatacgcacc ttttctagca ctttctgtca ctgtctttgc tggtcctgag gcccaagcag
    44941 ataattggac tgctacacca atgaagacaa gtaaactaaa cagattcaca ataagatgtg
    45001 gcaaaccaag aatttggcaa taaatcgaaa tagattgcgc gatattattc aattccattt
    45061 gtccttttgg tacgacattt gctacaacta aagcattcaa aatattgacg aaaaatataa
    45121 aaattaacgc ggcaaatact ccgcgaatat acgttttaac tggatttttc aaccgagtga
    45181 tatacacaga ggacatttcg ataccagtaa aaataaacat gatcggcgca aaataaacaa
    45241 atgaggatgc agtcgtagta tctggtatta atttgtccca tgaaaaactg cctaatgttc
    45301 cggttggtac gatcccaact ttgatccacg cagcaaaccc taataagagc atcatcgtca
    45361 aaggcacgta caatcctaac caaacaccga cttttccgcc gatttttgcc atatcaaatt
    45421 tcaaattcaa aaaagtgatg atccaataaa cgactaaaat gacaactaat gtaaatttgc
    45481 tgttcaaacc aagcggcaca ttatcgataa tattaccaaa caacggagca agtgctgaag
    45541 cgaccatcac catcccagga aacatttgga cccataacag ccatgagaca acgaaccccc
    45601 atttttttcc tagagcattc gatacccaaa gttctggacc acctttttgg ggaaacattc
    45661 cggcaaattc accagagatc aatgagattg gaaaagcgta caataatact gcaaccagca
    45721 tgtaaaaaat catcgtccag ctcgttgctg ctacatctgg aatatttcgt acacttgcta
    45781 caagggcgca ggtcattccg ataaaggtca tggtcgtgat ttgctttgat gaaccagtat
    45841 ctttcatctt tattccttct ttcaatagcg acatatgttt ttgttttctt ttatttttgt
    45901 tcaagaacag aaatgacaag gtttcatcga ttcagtacaa aacccagtat caccgaaaca
    45961 tccattgaac gatcttagtg aactgggttt gatacatgaa acgaacgatc caacttatag
    46021 aacgttttat aaaaaagaat atcttggcga ggaatatatg aaaaacaggg ggaaatacaa
    46081 ttgttttgat gaagcaaata attgtctgtt ctccatatgt ctcttgttat tctttcacac
    46141 aaaaatcagt tctattggat gatttcgttt agtttctttt ccattgcttt ggcgatttct
    46201 tttgtataaa aattgaaaat tcgatcatca ttcaaataag gagtcatgat agccgcacgt
    46261 agcagagtga ttttctgttc ttttttccat tcttctttcg ttagacccat tctttcaaca
    46321 aaacgaatcg gagaatcacc gtaatcttct tgtgtaaatg tagtatgaga agtaataaac
    46381 cgttcaccat aaacatcgcc atccatataa gaagaccgat cgaacatttt ttcatttaat
    46441 tcattgatag cgttcaaatc tgtacagcct tgttctttga atacccaatt gaccatatta
    46501 aaatcaggat gattcaatgg atagacttcg attgtttttc ctttaacagt aaaagtcaga
    46561 tgatcgagaa attctctgaa tctttgagct gcctcgattg aagccccaat caattgacca
    46621 tatccggtca cattcaatgg caatacacga tgtgctgtcc agactgcggc ggcggttgct
    46681 ccagcttttg atccttccaa aatataagct cctaacatat ctggagcctt tactgatttt
    46741 tcaaacacat atggcgcgaa ataagaaatg atatttctca tatttttatg tttgattacg
    46801 attcctccag cagcataagg gacatagccc atcttatgag gatctatcgt gacagaatcc
    46861 gcttctgaaa tagctctgaa cccttcatat acctctttgt caatcgttac gcagtggtgg
    46921 aaaacatgat gttcttcaaa gaattcagct aaggaagcat acggcacaaa ttctcctgct
    46981 tcgtttagaa acagtgaacg cgcatagccg ccatatgcgg catcaacatg taaatagaaa
    47041 tagatccctt catcttttaa cctttctctt aattggacga ttttatctac actgtctact
    47101 tgaccttctt ctgtcgttcc tacgacagca accacaccta gaattgggat tttttgttca
    47161 gctaattcac gaattgtttt ttccaaagca ttaatatcca ttcgataatc ctcttgaact
    47221 gggatagcaa ccatctgatc taagcctact ccgcaaatat ctaacgcctt catccaagaa
    47281 tagtgttttg tttgaggcac cagccatttc cctaatcttt ggatattttt tccgctacgg
    47341 gaagaagctg ctttgacctc atccatttct tcgtccgtaa agctttcggt catttccaaa
    47401 atttcttcaa cagataaatt caaaagttcc cactcagaca tttttttcac ttgttcaggc
    47461 agcacttcct tgacagctaa aggaatcgat ttgatgcagc gggcgtacca taatccttca
    47521 agattagcaa tagaaccatc tgctgcaatg tgtccccatc cgtctgtcat attgaataat
    47581 gaagcaaaat cctgtcctac ttctgcttcc atttgcgaag ttgccataga agattccaat
    47641 gcaacattat ttgggttcca cagcatcgca tagttgtaag ctaataaagc aggcatcaat
    47701 gtttcagcat tcatttgtcc ccagtaacgg cctgctgaat gccatgggat cgatcctgcg
    47761 cgtaatcgct gactgacttc atccaagacc tctatcatat ggtcgcgggt ggctagataa
    47821 tctggagtga gcttatctcc ttcgctaatt gcaggcatat ctgaaggaat ataattttct
    47881 ctccatccaa gatgttcatc taccatcttg ttcaacaaca ttttataaac tggtccattt
    47941 tcagctttgt cgcctataaa gacggccttg atatccatat ctttcatttt tcttccctcc
    48001 atattttgtt ggtcaatgtc tcgcagatac atgtgtttat ctgtaacacc tcttcactat
    48061 agcgccaaaa atgagaaatt tttttttata acatcaaatc cacgtagatt ttta
//
LOCUS       DACECY010000018        47942 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.019, whole genome shotgun sequence.
ACCESSION   DACECY010000018 DACECY010000000
VERSION     DACECY010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.019"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            382..570
                     /locus_tag="IWO38_001036"
     CDS             382..570
                     /locus_tag="IWO38_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ0313137.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            648..1661
                     /locus_tag="IWO38_001037"
     CDS             648..1661
                     /locus_tag="IWO38_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0313138.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1728..2762)
                     /locus_tag="IWO38_001038"
     CDS             complement(1728..2762)
                     /locus_tag="IWO38_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0313139.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2877..5102
                     /locus_tag="IWO38_001039"
     CDS             2877..5102
                     /locus_tag="IWO38_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ0313140.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5120..7003
                     /locus_tag="IWO38_001040"
     CDS             5120..7003
                     /locus_tag="IWO38_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ0313141.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6984..7958
                     /locus_tag="IWO38_001041"
     CDS             6984..7958
                     /locus_tag="IWO38_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ0313142.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            8617..9639
                     /gene="aroF"
                     /locus_tag="IWO38_001042"
     CDS             8617..9639
                     /gene="aroF"
                     /locus_tag="IWO38_001042"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ0313143.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            9672..10736
                     /gene="aroB"
                     /locus_tag="IWO38_001043"
     CDS             9672..10736
                     /gene="aroB"
                     /locus_tag="IWO38_001043"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ0313144.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            10738..11904
                     /gene="aroC"
                     /locus_tag="IWO38_001044"
     CDS             10738..11904
                     /gene="aroC"
                     /locus_tag="IWO38_001044"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ0313145.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            11921..13012
                     /locus_tag="IWO38_001045"
     CDS             11921..13012
                     /locus_tag="IWO38_001045"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ0313146.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            13025..14320
                     /gene="aroA"
                     /locus_tag="IWO38_001046"
     CDS             13025..14320
                     /gene="aroA"
                     /locus_tag="IWO38_001046"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ0313147.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            14313..14822
                     /locus_tag="IWO38_001047"
     CDS             14313..14822
                     /locus_tag="IWO38_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ0313148.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            14819..15655
                     /gene="pheA"
                     /locus_tag="IWO38_001048"
     CDS             14819..15655
                     /gene="pheA"
                     /locus_tag="IWO38_001048"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ0313149.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(15718..17454)
                     /locus_tag="IWO38_001049"
     CDS             complement(15718..17454)
                     /locus_tag="IWO38_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0313150.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(17451..18380)
                     /locus_tag="IWO38_001050"
     CDS             complement(17451..18380)
                     /locus_tag="IWO38_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0313151.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            complement(18380..18958)
                     /locus_tag="IWO38_001051"
     CDS             complement(18380..18958)
                     /locus_tag="IWO38_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0313152.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            19231..20517
                     /locus_tag="IWO38_001052"
     CDS             19231..20517
                     /locus_tag="IWO38_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0313153.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            20654..21523
                     /locus_tag="IWO38_001053"
     CDS             20654..21523
                     /locus_tag="IWO38_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0313154.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            21520..22407
                     /locus_tag="IWO38_001054"
     CDS             21520..22407
                     /locus_tag="IWO38_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0313155.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            22574..23257
                     /locus_tag="IWO38_001055"
     CDS             22574..23257
                     /locus_tag="IWO38_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ0313156.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            23254..24027
                     /locus_tag="IWO38_001056"
     CDS             23254..24027
                     /locus_tag="IWO38_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ0313157.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            24024..24422
                     /locus_tag="IWO38_001057"
     CDS             24024..24422
                     /locus_tag="IWO38_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ0313158.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            24607..25278
                     /locus_tag="IWO38_001058"
     CDS             24607..25278
                     /locus_tag="IWO38_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313159.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFLPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            25337..25777
                     /locus_tag="IWO38_001059"
     CDS             25337..25777
                     /locus_tag="IWO38_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313160.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            25789..26457
                     /locus_tag="IWO38_001060"
     CDS             25789..26457
                     /locus_tag="IWO38_001060"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ0313161.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            26521..27144
                     /locus_tag="IWO38_001061"
     CDS             26521..27144
                     /locus_tag="IWO38_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ0313162.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            27200..27586
                     /locus_tag="IWO38_001062"
     CDS             27200..27586
                     /locus_tag="IWO38_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ0313163.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            27799..29190
                     /locus_tag="IWO38_001063"
     CDS             27799..29190
                     /locus_tag="IWO38_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ0313164.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAVAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            29537..30184
                     /locus_tag="IWO38_001064"
     CDS             29537..30184
                     /locus_tag="IWO38_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ0313165.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            30577..33222
                     /locus_tag="IWO38_001065"
     CDS             30577..33222
                     /locus_tag="IWO38_001065"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ0313166.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            33532..34845
                     /locus_tag="IWO38_001066"
     CDS             33532..34845
                     /locus_tag="IWO38_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ0313167.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            34835..35488
                     /locus_tag="IWO38_001067"
     CDS             34835..35488
                     /locus_tag="IWO38_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0313168.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            35543..36235
                     /locus_tag="IWO38_001068"
     CDS             35543..36235
                     /locus_tag="IWO38_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAQ0313169.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            36385..36585
                     /locus_tag="IWO38_001069"
     CDS             36385..36585
                     /locus_tag="IWO38_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0313170.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(36839..38518)
                     /locus_tag="IWO38_001070"
     CDS             complement(36839..38518)
                     /locus_tag="IWO38_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ0313171.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(38520..38732)
                     /locus_tag="IWO38_001071"
     CDS             complement(38520..38732)
                     /locus_tag="IWO38_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ0313172.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            39125..40855
                     /gene="efrA"
                     /locus_tag="IWO38_001072"
     CDS             39125..40855
                     /gene="efrA"
                     /locus_tag="IWO38_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ0313173.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            40852..42612
                     /gene="efrB"
                     /locus_tag="IWO38_001073"
     CDS             40852..42612
                     /gene="efrB"
                     /locus_tag="IWO38_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ0313174.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            42707..42904
                     /locus_tag="IWO38_001074"
     CDS             42707..42904
                     /locus_tag="IWO38_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313175.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            43057..43542
                     /locus_tag="IWO38_001075"
     CDS             43057..43542
                     /locus_tag="IWO38_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ0313176.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(43646..44239)
                     /gene="clpP"
                     /locus_tag="IWO38_001076"
     CDS             complement(43646..44239)
                     /gene="clpP"
                     /locus_tag="IWO38_001076"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ0313177.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            44419..44946
                     /locus_tag="IWO38_001077"
     CDS             44419..44946
                     /locus_tag="IWO38_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ0313178.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            45037..45774
                     /locus_tag="IWO38_001078"
     CDS             45037..45774
                     /locus_tag="IWO38_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ0313179.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            45778..46695
                     /gene="miaA"
                     /locus_tag="IWO38_001079"
     CDS             45778..46695
                     /gene="miaA"
                     /locus_tag="IWO38_001079"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ0313180.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            46697..47926
                     /gene="hflX"
                     /locus_tag="IWO38_001080"
     CDS             46697..47926
                     /gene="hflX"
                     /locus_tag="IWO38_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ0313181.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        15378 a         8402 c        10395 g        13767 t
ORIGIN      
        1 gttagtctac attattttgg cgatttgcca ataaggactg ttgagttgtg caaaatcaaa
       61 tctgggtcag aatgaacgtc attctgtctc agatttttcg ttatttcaat ttgattaact
      121 gattgatcaa aaagattttt attttcatat tctcaaatga ttcaacacta aatatacatg
      181 tttgacgcgg aaattattat tcgattttgg cggcatcagg ggggatgacg acgtagcaga
      241 cccattattt tactcaagaa aatgtccgtg cctattattt agaatacgat agtgaacgtg
      301 ctgggagttt tgttctttta gagaaagtct ctgaagataa attagttgtc ttagattttg
      361 actgtaacga aaaatggcga aatggaagag caccagcaag tgattgaccg gatctacgaa
      421 gcgacacgat atctgccttt ggatcgtttg tgcttaagtc ctcaatgtgg atttgcttca
      481 acagaagaag aaaatgtttt gtttgaagaa caatggaaaa aatttctttt gtcaaaagca
      541 ttgcagaaga agtttgggga aaatcattaa atcatctagg agagcttgat aggaaataac
      601 actatggtat aatcagaatg cttaatacta actactgagg gagccttatg aaaaagaaaa
      661 ttcttaaatt tatcggatgg ttcttcggaa tcattcttgg gatcgcgttt gcaatctttc
      721 ttgccttcca gctttccccc agacctggag cattcttgat caaccatatg ttttcaaata
      781 ctgttcaaat cactgataaa aaaacgttcg atcaagcaga aaaaaacgtg gcgaaaaaaa
      841 cagatttgac gtatgagtcc aagtataaag agaatactta tgacatctac tatccaaaaa
      901 acagcaaggg gcctgttcct gttctatttt gggtacatgg tggaggattc gtcggcggag
      961 ataaatcggg tgtcaaagaa tttgcgacaa aattagtttc agacgccaac atcgcagtgg
     1021 ttgcgatgaa ttatgaatta gcgcctgatt cagaataccc taatcaagtt cttcaagtga
     1081 atgaactgat caaagattta ttagctgaag aaaaagacga caaattgcta gacatggaac
     1141 aattattttt tggtggagat agtgcaggtt cacaaatcgc attgcaatat gcagctatac
     1201 aaacgaatga tgcgtatgct aaacaaatga aaatcaaaca gctacttcca aaagatagtc
     1261 tgaaaggaac catttcttat tgcggacctg ttgatttgaa gctaacagca cagcagcatt
     1321 cggatgatcg atttatgaag tttttcgtca aaaccgttgc gtggtcgttg attggaacaa
     1381 aagattggaa aacgagtgag caattgaaag aagctagtgt ggctgaccat gtttctaagg
     1441 aatttccgcc aacgtatata actgacggaa atgcatactc attccaggag caaggtcaag
     1501 cattagaaaa tcggttgaca gaattaaagg tacccgtgca atccttgttt tatacagata
     1561 caaaaaaaga gattacccat gaataccaat ttgattacac gctaaaagaa tcgcaaaatt
     1621 gttaccagca gacattggat tttgtgaata agtataagta agaggttgtg aaaaagccgt
     1681 ttcgttttga gtatgaagaa acgaagcggc ttttcacaac ctctctttca ataccttttt
     1741 gttcttggga taaaactttc cctaacaatc aatcgtgcag gataagtcaa aatgattggt
     1801 tcgtcccgta taccgtcaat tcgctccaat gccattcgaa cagctgcttg cccaattgat
     1861 ttcgcattga tttgaacact ggtcaagcca ggggagaggt ttgcgattgt ctcgatatta
     1921 tcaaagcttg ctacaacaat ctcttctcca atttggatat ttttttgttt caatgcccga
     1981 tagacaccaa ttgccattgg atcgctcccg acaagaatgg cagtaggaag aggggattca
     2041 ttttctaata gattagtcgc aagtacctca ccttgttttt ctgtccagcc acctagatga
     2101 taagacatat atttttctaa gtgatgatcg atcataaaat gctgataggc acgcatcctc
     2161 ttttcattta tagaaatctg cttcccaccg aattcatcca tatcgacttt atatcccccg
     2221 atataggcta ttcttttatg accattttct aagaacaatt ccagtacggt tttagtcatc
     2281 cgttcaagat ctgcatacac catatcgacc tcttgctgga tgtcagggtt atccacgacg
     2341 actatttgca tgttttgttt taacaaggcg gtaatcgatt ctttcgtaac agtcccaaca
     2401 acgatcaccg cacctaactg atctagatct tcccattgtt taggattatc agatagatta
     2461 tagattcgat tcatccctaa atgcagacgt cgagcttctt cttccatccc tactcgcaaa
     2521 tgtttgtaat agatatcctg ttcttcgctc gctttgccga tcgtagagat cacaccaatc
     2581 gaaggcattc tctttttgat atatttacga gtactgatgt cataatccaa cgcagcagct
     2641 gcctctacta tccgttgccg agtagtctga gccacatgga tcgtctgatc gttattcaac
     2701 acacgggaaa ctgtagcagg agagacattt gcctttcgtg cgacatctct aattccaacc
     2761 atttttacac actcctcaaa aaaataaaaa atttttattg acaaaattgt ttagtaaaca
     2821 gtatacagga attataaaaa aaagtaaacg gtttccgttt tgttgggagt gaaatgatgg
     2881 atattacgca tagaaaaaca gataaaaatt tcatcttatt tgatgaaaag agccgagtct
     2941 tccatttacg aaatacattt ctttcttatc tgataaagat agaagaaagc aatgtattgg
     3001 cgcatattta ttttggcaaa ccagtcaagc agtacaaaga caataaaaat tatccccgaa
     3061 gagatcgtgg attttcagga aatgttccct taaatcctga ccggtctctg tcgaaagata
     3121 cactaccgca agaatactct tctcatggca gcatggactt tcgaacgccc gcatcgatca
     3181 ttcagcgcaa gaatggatca gacttactgg atctgagata tgacagccat tatattacag
     3241 atgggaagcc agagatcgaa ggcttgcctc aaacttatgt gatggacaag tcagaggcac
     3301 aaacgttggt gatttcactt aaggatagag aaacagcgat ttatttcgat ttgttctata
     3361 ccatatttac tgatagagca gtgatcacac gatctgtaaa gataagaaat gaaaccggag
     3421 aaacaatcaa actagaaaaa gcggctagtt tccagctaga ctttgcccat actaggcgat
     3481 ttgatgaagt cattgctttg ccaggagcac atgtcaatga aagacagatt tcacggcaat
     3541 ctgttttatc tggaacaaaa gtatttgaaa gccgtcgagg aacgagcagt caccatatga
     3601 ataactttat tgctttagtt catcatcata cgacagaaaa tactggagaa gcgatcggac
     3661 ttcaatttgt ttattccggc aaccattctt tcgaacttga aaaagatcag atcaatcaat
     3721 tgagagtcgt cggtgggatc aacagccatc gtttttcgtg ggaattgaat gcaggccagt
     3781 cttttcaaac acctgaaatg atcctttctt actcaagtca gggattgaac aaaatgagtc
     3841 agatccacca tgagttgcta cgtgaaagaa tcgcaagagg tcgtcatcaa tttgctgaac
     3901 ggccgattct ggtcaataac tgggaagcca cttactttga cttcaatagt gaaaaaatca
     3961 aggcgatcat tgatgaagca aaggagctag gcatcgaaat gttcgtatta gatgatggct
     4021 ggtttggaaa aagagatgcg gacaattctt cattaggaga ctggttcgag tatgaaggca
     4081 agttgacaaa tggattacgt gaaatagcag attatgctca tagcaaagga ttgaaatttg
     4141 gtttatggtt tgaacctgaa atgatctcgg ttgattctga actgtatcga acacatcctg
     4201 attttcttat gcaggagcca ggtagaatgc cttctgcttc cagatcacaa catgtactgg
     4261 attttacaag gttagatgtt cggcagacga ttgagaaaca aatgagaaag attttagata
     4321 cgattcctct tgactacatc aaatgggata tgaaccgttc gctttcagat gtctattcga
     4381 ttacccttga cccacagaga caaggagaag tagcgcatcg ttatatgctc gggctttatg
     4441 aactacttga acatttgaca actgattatc cggaaatctt atgggaaggc tgttctggtg
     4501 gaggtggacg gtttgatgcc gggtttattt attatatgcc gcagtcatgg acaagtgata
     4561 acacggatgc agtggagcgt atgaaaatcc aatatggaac aagccttgct tatccgattt
     4621 cgtccatcac agctcatgta tcagctgtac caaatcatca gaccgacaga tctacttcgt
     4681 tgaaaacaag aggagaaaca gctatgagcg ctgtttttgg ttatgaactg gatttgacaa
     4741 agctgtctcc tgaagaaaaa aagcaagtaa aagaccaaat tatatcctat caaacgattc
     4801 gtccggtgat ccagtatggc cattattatc gtttggcgag tccttttgaa gaaaatattg
     4861 ccgcatggat gtttgtgtct cctaaacaag atgaagctat tgtgttttta gggaggatac
     4921 tagcttcagc tcaaccagcg ttccatgaag tatatctgat ggggttagat gatgaggcac
     4981 tttatcagga acagacctcg aagcggatat tttcgggggc cgaattgatg acagttggac
     5041 tttacttccc cgattttcaa ggtgatttcc aaacagaact gcttcatttc aaaaagttat
     5101 gagagagaag gaaaaaagta tgaaagcaaa tataaaatgg ttagatgatc cacaagtttt
     5161 tagagtgaac caatcaaatg cacatagcga tcatgtttgt tatgcaacaa gggaagaagt
     5221 agaaaccaca tcctccctta aacagtcttt aaatggatca tggcgttttt gttattcgcc
     5281 aaatccttcg gtacgaccaa aaacatttta taaaaaagat tttgatgcct gttcttttga
     5341 tgaaatcact gttcctagtc atattgaatt agcaaattat gacaagatcc attacatcaa
     5401 tacgatgtat ccgtgggagg gacatgtatt tcgccgtccc cctttcacga caagccctga
     5461 gaaagaggcg gaagggatct tttcagctgc taaagacaat ccggttggtt cgtatttgac
     5521 gacttttcga ttagaagaag gtctaaaggg aaaacaaatc tcgattcatt ttgctggtgt
     5581 agaacaagca atgtttattt ggttgaatgg tcattttatc gggtatgccg aagattcgtt
     5641 cactccgtct gaatttgatt taacacctta tttggaagaa gaaaatattc tagctgtgga
     5701 ggtttacaaa agaagtacag ctgcattctt ggaagatcag gactttttcc gattttttgg
     5761 gatcttcaga gatgtcttct tagttgctcg aggacaaaac catttagaag atctttggat
     5821 caaacctcgc ttagatgaca aatgtcaaaa tggcaaactt gaagtagatt tgtcttttga
     5881 gcaagtagcc aaaaacagtc agctgaatgt agcgatacta gatcctgatg ggaaggaaat
     5941 ctatcaacag caatatccgt taaaagagca aatgacgatt cgcatcgatg gatttgaaaa
     6001 tgtggcactg tgggggcatt ttcagcccaa tttatataag cttgaattgg agacgataga
     6061 cgaagaaaaa agagtaacgg agtatattga ctattccttc ggttttcgta aaatcgaaat
     6121 caaagataaa atcatctact tcaatggaaa acggatgatt atcaacgggg taaatcgaca
     6181 tgagtggcat ccgaaaaaag gacgcgccat cactatagaa gatatggaaa aagatttggc
     6241 tatcataaaa aatgcaaaga tcaacggcgt acggacatct cattacccca accagatcac
     6301 ttggtattat ttatgcgacc aagcaggaat ccatgtgatg gcggaaacga acctcgaatc
     6361 ccatggctca tggcaaaaaa tgggcgcatt tgagccttct tacaacgtac caggtgcagt
     6421 acctcagtgg caggcggctg ttcttgatcg agcaaaaacg aatttcgaaa cattaaaaaa
     6481 ccatccaagt attctttttt ggtctcttgg aaatgaatct tatgcggaag aaaacattcg
     6541 acaaatgaac gattacttca aaaaagtaga ccccgatcga cttgtccatt atgaagggaa
     6601 ttttccaaat ccagcattcg aagattcgat ttcagatgta aaaagctgca tgtatgcgcc
     6661 accagaagag atcattgact accttgaaaa taaagcggaa aaaccgttca tcttatgtga
     6721 atatatgcat gacatgggga attcgttagg tggaatggat tcttacatga cgttgcttga
     6781 tcgttatgaa atgtaccaag gaggctatat atgggatttt aaagatcagg cgatccaagt
     6841 gactgatcca atcacaggca gagaagtgat gcgctatggc ggtgactttg acgataaacc
     6901 aagtgactat gaattctcag ggaatgggat cgtttttgca gatggacaag aaaaacccgc
     6961 catgcaggag gtaagatatt attatgaaaa atacagtaaa taaaagtcat atggatacgg
     7021 aaaaagttgc aatcgtcttc ggcgactgta cattaggtgt caaatcgggg aatacgcatt
     7081 atattttttc ttatacaaga ggcggactgg aatcgctcaa taaaaacgga aaagaatggc
     7141 tataccggga aacacttcca gttttttggc gagcaacgac cgataatgac agaggtagcg
     7201 gatttggatt taaatccggt atgtggttag gagccggatt gtatccaaaa gtcaaagaaa
     7261 tagaagtact ggtggacaat aaaaagatcg atttgccgat tgctccagta aataatcaat
     7321 attcaagtaa tgaatacgct gaaacggttg aaatcatttt tacactggaa atcaacacag
     7381 taccaaaaac ccaagtgaca atttcctacc aaatcgctgg agagggtaaa atgcttgtca
     7441 atatgcatta ttatggacaa aaaggattac cggaattacc cgcattgggt ttaaggttca
     7501 ttatgccaac gaaagcaaaa ttttttgagt atgaaggcct atcaggggaa acatatccgg
     7561 atcggatggc tggtggtgtc gaaggtattt ataaagtggc aggactcccc gtttccccat
     7621 accttgttcc ccaagaatgc ggcatgcata tggaaacgaa atgggtagaa ctaacaagaa
     7681 atcaaacgtt ggataatacg gataaagatc aagaagattt ttcgttgcga tttgaacagc
     7741 aggatacgcc atttgctttt tccgctttac cattcaaagc agaagaactg gaacaggcaa
     7801 cacatataga agaactaccg ccagaaagac gaacggttct tacgattttt gcaaaagtaa
     7861 gaggagtcgg gggtatcaat agctggggag cggatgttga gtcaccttac cgaatcagca
     7921 gcgaagagga ccatgaaatc ggctttatta ttcagtagca atctttagat tattatttga
     7981 aaaacggagt ttacaagaca aagctgcttt attctatatg tcatgaacaa gagagtaaaa
     8041 aagttctcag atacttttgt tcatgactat tttcttcaat aaaaccaatt gttatttttc
     8101 tgaaaaacaa agttaactat cagaaaatta aaaaaagttt cataaaaatg attgacagtc
     8161 gattttttta tgtctatact aggtacaatt cttacatcag tttggagcga ttttttatga
     8221 aatctagaaa acttacaaaa ctgaatacgt attactttag ctattttaga taggtttgta
     8281 ttcatgactt cgtgggtaca aacaaaccag gtttgtatct atgatggcta aagattttta
     8341 aacacgactt acagccacgt agagaaattc atctatagtg gctattttat atttttgcag
     8401 cgctttttaa ggctcgttga agatgttcac tgtagaggca atttcctaca gggacatctt
     8461 tttttgttga ttatctgggt cataagtagg aaagaaggag cgaaaatgag aacaacagga
     8521 cacggtcatt tagtaggaat taatgtaaaa ccaccttgaa aaagtagaga aaatcagcca
     8581 agatagataa gtaaacaaaa gaaagcggga aaaacaatga tagtaattat gaaaaaagaa
     8641 gcaacggaag aacaaataaa aaaagtcgtc gatcatatcg aaaaagaagg attcaaagtc
     8701 tcaatcgatc aaggaacaga acgagtagtg attggactaa aaggtgatac gcgaagttta
     8761 caagaaggag catttacacg ctatgaagga gtagaaaatg ctgtgcgtat tttaaacaca
     8821 tacaaattga cttctcgaga attccatcct ggcaatacag tcgtggatgt agacggagta
     8881 aaaattggtg acggcagttt cgtgacgatg gctggtcctt gttctatcga aggattagac
     8941 caaatcagag aatgtgcgcg aatggccaaa gccgggggcg caaaaattct gcgaggtggt
     9001 gccttcaaac cacgtacttc tccatatgct ttccaaggtt tagaagaaga aggattgaaa
     9061 tacatccgtc aagcggctga tgaatttgga atgaaagtga ttactgaagt gatggatgaa
     9121 gggcatatcg atatggtggc tgagtacagc gatatcctac agattggcgc acgtaatatg
     9181 caaaatttta aactgctatc tgctgtcggt aaaacaggta aacctgttgg attgaaacgt
     9241 ggaatttccg gtacgatcaa tgaatggctt aatgctgcag aatacattgc agtcgaagac
     9301 aaatctcctg tcatttttat cgaacgtggg attcggactt acgaaacagc gacacgtaat
     9361 acgtttgatt taagtgctgt cccattgatg aaaaaattaa ctcatttccc tgtgattgtt
     9421 gatccaagtc atggtacagg aatttgggaa cttgtaccac caatggctcg tgcgggtgtg
     9481 gcaagcggag cagatggcat gatcgtagag atccatcctg atcctgcaaa tgcttggtca
     9541 gacggtcccc aatcgttgaa tgaaaagact tactcacgca tgatgaaaga agttgcgatc
     9601 atgaaagaag caatggaaaa gattcagaca ctcgattaat tccagctttt taaaagaaag
     9661 aagtgaaacg aatgcttact gtacatgttg ataaccaaaa atacgatatt catattgaaa
     9721 aaggcgcact agaacaggct ggatcatggc ttcaaacgat ttggagacct caaaaagtag
     9781 ctgtgatcac tgatacaaac gttgctccac tatatgcaga aaaaatcatg ttccaattga
     9841 gacaagctaa ttttgaacca gtcctttcaa tcgtaccggc gggtgagacc agtaaatctt
     9901 tagaacaagc agctgagtta tacgattttt tagcagataa tgattttacc cgcagcgatg
     9961 ggatacttgc acttggtgga ggaatggtgg gtgatctagc agcattcgtg gcctcgacgt
    10021 tcatgcgtgg gatccatttc gttcaaattc cgactacgct acttgcacaa gtagattcta
    10081 gtattggcgg gaagacagca gtcaatacaa acaaagcgaa aaaccttgtc ggaacattta
    10141 gccagccagc tggtgtattg attgatcctg aggttctaaa aactttgccg gtacgtcgtt
    10201 tacgagaagg aatcgctgaa atcatcaaat cagcagctat agcagatgca acgctttgga
    10261 aacagctgaa cgagctgaaa gacgaaaaag atcttctgga acatgctgtt ccaatcatta
    10321 ctgctacgtt ggaaatcaaa cgaaaagtag tggaagaaga tgtctttgac caaggaaatc
    10381 gacttatttt aaattttggt catacaattg gccatgcaat tgaaaataca gcaggatatg
    10441 gggtagtcag tcatggagaa ggagtagcta ttggaatggt agccatcact tctcatgcag
    10501 aaaaaatcgg cttgtctcca actggatcga cagctgcttt agaaaggata cttcaaaaat
    10561 tccatctgcc gattacgtac gatctaagtc atccagatga attattccac gcgataaccc
    10621 atgataagaa agcaagagga agccaattga atatcatctt gctagaaaag attgggcaag
    10681 caaaaatcgt tcctgttccc acagagacat tcaaagatta cttaggagga ctgtaagatg
    10741 cgttatatga ctgcgggaga atcccatgga ccagaattaa cagccattat tgaaggacta
    10801 ccagcaggat tacctctgtc agaagaagaa atcaatcacg aactcgctcg cagacaagct
    10861 ggttacggca gaggaggaag gatgaagatc gaaaaagatc gtgttcggat cacgtctggc
    10921 atccgtcacg gcaaaacaat tggttcacct gtcactttag tggtagaaaa taaagattgg
    10981 aaaaattgga caactgtcat gtctgtagca gaagtgcctg aaaaagacca atcactgcgg
    11041 cgagttgcac gtccgcgtcc aggacacgcg gatttggtag gcggtatgaa ataccatcat
    11101 cgagatcttc ggaatgtgct tgaacggtcc tctgcaagag agacgaccat gcgtgtagca
    11161 atcggcgcaa ttgccaagaa aattctggaa gaacttgaca tcatcgtagc aggacacgtg
    11221 atttgtcttg gtggaatcca agcagaaata cctggaaata tctcgattca agaaatcaaa
    11281 gaaatttctg aagcgtcaga agtacgagtt gttgatccag cagttgagca aagaataaaa
    11341 gatttgatcg atcagacaaa gaaaaatgga gatacgatcg gaggaattgt agaagtgctc
    11401 gttggaggcg ttccagctgg attaggaagc tacgtacaat gggacacgaa gctagatgcc
    11461 aaaatcgcac aagctgtggt tagtatcaat gcctttaaag gcgtagaatt tggggtcgga
    11521 ttcacttctg gttcattacc aggctcagaa gtgatggatg agatcctatg ggaagaagaa
    11581 agaggctatt tccgacgtac caataatcta ggcggcttcg aaggcggaat gaccaatggc
    11641 gaaccacttg tcattcgcgg ggtcatgaaa cccattccta cattgtacaa gccacttcaa
    11701 agtgttgata tcgatacaaa agaaccttac aaagcaagtg tagaacgttc ggatagtaca
    11761 gcagttcctg cagctagcgt cgttgcggaa aatgttgtag ccactgtggt agcgacagag
    11821 ctattgtcaa aattcccaag tgattcgctt gttgagttga aagctgcagt gaaggagtac
    11881 cgtgactact tgaaacatta ctaaaaaggg ggcaaataag atggcacaac atgtttttat
    11941 cgtggggctt ggactgattg gtgcttctat ggcactttgt attcgtcaga ccaatccaga
    12001 actgatagta gttggttggg atagtcagtc aaaaacccgt gagcaagcag tagaacagca
    12061 gatcgttgat tacgttgcaa gtgattttga aacaggtgca gaacaagcag acgttatctt
    12121 attagccgtt cccgtccgta caaccttagc ttatcttgat gtccttgaaa gaatcaactt
    12181 gtctgatcac gtcatcatta cggatgtcag cagtacgaag cagcaagtcg tgacatccgc
    12241 tgagcagaaa aatcttcggt ttgtcggtgg acatccaatg gctggttctc ataaatcagg
    12301 tatacaggca gcagatgcta aactatttga aaatgcctat tacatcttta cacctttgga
    12361 aaagacgaaa aaagatgttg aaaaactgca agaactattt tgcggaacca aagcaaaata
    12421 tgtaactctt acagcaagag aacatgaccg tataacagga atgctaagtc attttccaca
    12481 tattctggca gctggcttag tcaatcaggc agaacaattc aatcaagagt atccgcgagc
    12541 caaacaactg gcagcaggag gctttcgaga tatcactagg atcgcttcct cagatcctgt
    12601 catgtggaca gatattttgt tgagcaataa gcagatatta ttggaacgat tggcagattg
    12661 gcaacaagaa atgacacaaa tagcagaatg gatcatgact gagaaccaat cggaaatctt
    12721 ttcttttttc aatcgggcaa aagaaagtcg agaccagttg cctgtccata agcaaggcgc
    12781 gattcctgcc ttttttgatt tatttatcga tgtgccagat gaacctggcg ttattgcaga
    12841 agtaactgga ctgatcggaa aagcgggagt ttctttgatt aatttgaaga tccaagagac
    12901 cagggaagat attttaggaa tactgcaaat cagttttaaa aaccaacagg atttgttaca
    12961 agcaaaaaga atcattatgt ctaataccag ttatcattgc tggatcaaat aggaggctgg
    13021 ataaatgcaa ttattacaac aaatacatgg attaagaggg actgttagga taccagcaga
    13081 taaatcaatt tcccatcgca gcatcatgtt tggagcaatt gctgaaggaa cgacgactat
    13141 acaaaatttt ttgcgcgcag aagattgtct gagtacttta catgccttcc aacaattagg
    13201 tgtcgagatc gaagaagagg aagaggtgat caagattcat ggtcgcggta gccactcatt
    13261 tgtccaacca accgaatcaa ttgacatggg aaactccggt acgaccatac gtttattgat
    13321 gggtattttg gctggacagc cttttacgac tactctgttc ggggatgctt cgttgtctaa
    13381 acgtccaatg gggcgagtga tggaaccttt acgcgagatg ggcgctgact tgcaaggaga
    13441 agaaaatgac cagtatctac caatcactgt gacaggaacc cgctctttat cacctatccg
    13501 atacaatatg cctgtagcca gtgcgcaagt caaatctgct ttgctgtttg cggcattaca
    13561 agcagaaggc acatccgtaa tcgttgaaaa agaacgttcc cgtaaccata cggaagaaat
    13621 gattcgtcaa tttggcggaa ggatcacagt ggaagataaa acgatcatag tgaccggacc
    13681 gcaaaaatta actggtcaac agataactgt tccaggtgat atttcatcag ctgctttctt
    13741 tctagcagca ggacttcttg ttccggaaag ccaactgttg ttaaaaaatg tcggggtcaa
    13801 tccaacaaga actggtatct tagatgtgtt ggaggagatg ggcgcagcga ttacccagac
    13861 gaatcacaat gaacagaacc aatcggctga tttaagtgtc aagacgagtc atttgaagaa
    13921 agcaacgatt ggtggagaaa tcattccgcg tctgattgat gaattgccca ttctagcttt
    13981 agtggctact caagcagaag gaatcacgat catcaaagac gcagaagaat taaaagtaaa
    14041 agaaaccaac agaattgatg cggtggcaga agaattacag aaaatgggag caaagataaa
    14101 agcgacagca gacggtctga tcatccatgg gccgacacct ctgcatggcg caaaagtgtc
    14161 cagcagaggc gatcatcgaa tcggtatgat gctgcaagta gctgctttga tagctgatgg
    14221 accttgcgaa ctagaaggag ctgaagctgt ttcaatctct tatccggcat tttttgacga
    14281 tttagcagaa cttgtgtcag gaggagaagc tcatggctag cattttgctg attggtttca
    14341 tgggcgctgg aaaaacaaca atcggtaaag ggttagctca atgtttacaa aaagagtatg
    14401 tagatttaga tacaaagatt gaggaacata tccaattgtc gattgccgaa tattttcact
    14461 attacggtga aaaaagcttt cgaaaagtgg agtcagatat tttaagaaag ctgtcaaatg
    14521 aagacaaaat tatcgcgact ggtggaggga ttgtccaaag tgcagaaaat cggcgttttt
    14581 taaaaacaca gccgattgtt ctttacttag aggcagaagc ggactgtcta gttgatcgta
    14641 tccaacaaga tgaaacgtct attcgacctt tagctcttgg aaaaacaaga gaagaaataa
    14701 aagcattatt ggcacaacga ctttcttggt atgaagaaag tgcgacacat cgaatcaaca
    14761 cgaccaatca aacaccagaa aagatcatcg ataccattat agaaaggatc aaaagcatat
    14821 gaacgtcagc tatctagggc cagaaagttc attcacttat caggcggctt gtcagctatt
    14881 tcctacagaa caattgactg cgtatgcttc catccctgtt tgtttgcgcg ctttgtttcg
    14941 caaacaagtc gatctggctg tcgttcctgt agagaattca ttggaaggag ctgttcatca
    15001 cacaatcgat cttctttcca aacatccaga agtcgaagtt aaaagtgaaa tcgttcttcc
    15061 tatcaagcag cagcttttag gcaatccagc aacaaagatt acaaaaatcc tttcacatcc
    15121 acaagcacta gcacaatcac aacagttttt agaaacgcat tatcctaatg ttcctttagt
    15181 agcaacagaa tcaacaactg ctgcagcaat gtatgtagca gaacatccga aagaagacgc
    15241 agcagcgatt gcttcattag aaacagcaca gcatgttgga ttggagatct tagctgagaa
    15301 tatccaagat aatgagttga accagacacg tttttggatc gtcggtgacc gaaagatgac
    15361 cagccagcag tcagctccag taaaaatgag tgtcatcttg acactacctg ctaatcggcc
    15421 agggatgctt cataaaatgc tggcagcttt tggctggcgg gaaatcaact taagtaagat
    15481 tgaatcccga ccattgaaaa ctagcttggg agaatatttt ttcgtgatcg atttgttgtt
    15541 ggaccgtcca atgacattag tagagaatgc gctccaagaa atcaagatgc tcggaggaga
    15601 aagtcaaata ttgggctgtt atccagtgct gacagtagaa gagacgcgta gatgaaagtc
    15661 aagaatactg attgatttat ttaacttaaa ataaaaaaat cagcgcttgg ctattcttta
    15721 tttttgatat tctgattgat ttttaggaag aagcggtaga caaatcgtta cagtcgttcc
    15781 tgtcccaatc actgattgga tcgttaagcc atatgcatcg ccatacatta ggcgtatccg
    15841 ctcatctaca tggctgatgc cgattcctga aaaatgatca tttttttgtt tggtattttc
    15901 agtatcgaag ccatcgccgt tatcaatcac ttcgaataaa agaagctgat tttttgtttt
    15961 gccgtacacc gtgatgaatc cttgcgtttt attgtgaaaa gcatgaagat aggcgttttc
    16021 gataattggt tgaaggatca tattcggcat agataatgac tgcagttcat caggaatcat
    16081 tattttagcc gagattcttt cgccatatcg catactcaaa agatcgatgt aactttgtac
    16141 gcttttgatt tcttccttga ctggaatcac ttcttctttt ttcccaatcg tacttcttag
    16201 taaagtaatg aaactgtcga ttccttgaac tgcttcatct gtttttttct gccaaatcaa
    16261 aaatttcaag ctagtcagcg tattataaat aaaatggggc tgtatctgtg cttgcatagt
    16321 ttggatttca agcgaacgtt tcttcttttc gtcttggatc gtctgttcat aatgatgctg
    16381 agcattactt agcaattgat tgatcgtatt ccctagcaat tggacttcat ggcttccttg
    16441 tacaggaaca gtagctttta agccatcttc ctctagccgt tttagcgaat caatcaagcg
    16501 gtagatcgat tttgtgtgtc gatccaaata cataaatacc aatgagccag caaaagcaag
    16561 tgtcacgata ttatacaaaa taatcggaaa aatcacgccc atttggttag ataaagaatg
    16621 aagatcgact tgactgagta atgtacaatc ctggcggtac agaggaagac gggtcaactg
    16681 ctgtccattt gaaatcgttg tttcacctac tgttagtttt ttgaaaatca ttgatcggct
    16741 cccaattgta ttttcttcat ttgatgcgat gatcgtctgt tcttcgttca tgaaggagat
    16801 cttcgatatc tcaggattta ctacagattg aaaaagattc gctagttgct tggatgagat
    16861 cgataaaaca gcatatccgt aaacgtgatt caatgtattt ttcagttttt tgacaaagaa
    16921 gacgctgggt gctttcttat cgcgaagagt caatccgtgg tgtgcaaaaa gcatttgtgt
    16981 atcagcagca cgttcattca ctctctgagc aatcgcagat tgaaaaaaag aatcggcaga
    17041 tacggcagtg agcatgtcgt ttccgacaaa agttcttcca ttttctccaa agatcaatac
    17101 atcgtaatcg atcattggat ggatttttga aagagagtca ttcatttttt ggattgttct
    17161 tagttgctga agctgttcca tatgttgggc tggatcattg gaaaaatagc ttttgacagc
    17221 tggattagaa tcaaaagcgg ctaatgtatt ctcgattgta tcattcaaac tttcaaattc
    17281 agattgaatc ttttttagta ccacatcatt cgattgattg aacagctgga tataggcatc
    17341 ccttgatcga tgataaataa acaaagaaat gacgatggct tgcagaaaaa gaccgatgaa
    17401 caaaatgaac agaagtttgg caaagagttc atgctctttg attttttttc tcattttaca
    17461 aaagttcctt tacggtattg agaaggtgtc attcctgtct cttctttaaa aactctggag
    17521 aaataaccca gttcggtata gccgactttc tcacagattt ccgtcaaagt aagccgtgtt
    17581 tccgtcaaaa agagcttggc tttactgata cggacttttt ttagatattt tgaaaagttg
    17641 ataccaaatt tttcagtaaa gatggctgag agatagctat atgaaaaatg atatttttcg
    17701 gataaaagtt gtaaattgat ttctttctca taattttcct ggacgaactc gtagatcaaa
    17761 gtcaagatat cgaaattgtc tttttttgca tttgcagaaa agtgggtgag tttctcaagt
    17821 gtttctttga caagaataga aaaatcttcg aaatactcag catcggctaa taaagaagga
    17881 agggcgatct tgagcaaaga aagttcttca ttcccaacat gctcgtcagc caaagcgttg
    17941 agtaatgcat agaaacgatt gattgcctca tgtttcaaat aagctggatc aataggctgt
    18001 tccaatatcg tcgtgaagaa attctcaagt tctttgacag ctaaagcgta gtctttatga
    18061 acgatttttg ttaaataact acttgcttgc tgctcgtggt tggttaataa cggaagaagt
    18121 tcatgctgat aagcaaggtt ctttccctta tgatagaaac gttgtccttt ggtgcaattt
    18181 ctgatctctt ggaatatttc attgatatct tggattgcaa aagaatcaga aacaacaaaa
    18241 aaatcacctg gaaaaacttc ttgacgaaac ttttgaacaa acttcctaag tgtatctttg
    18301 gaatcagtat tgattacata gccggattct tccgaaaagg tagaaacagg ataaatagta
    18361 aacgaatcgt cgctatccat caatgaatgg gtgatcgact gtttttgttt agagccttgt
    18421 tttgaactga atggccgttt ttcggtatat aacagaaaaa caaggtgtgt tcaaaaagaa
    18481 gagggtcaag caatggacga gtttgtccag caatctgctg tgaaagattc tcgcagatct
    18541 gctcataacg attggtgatt gggcttttgt tgggcttttt tttgattttt tgtgaaacgg
    18601 cagttaagga atttagaaaa aaatcaggac tcaatcgagg ctttaaaaga taatcagcga
    18661 cgccttcttt aaaagaatct ttaaccatat caaattcatc atagctagag aggataagga
    18721 tctgtgtttc aggttgttgc tgttttaata gccgcgataa ttcgattcca tttaattcag
    18781 gcatcattac atctgtgacc acgatatcga ttgcatgtac caaagaaaaa tcaagcgctt
    18841 tcagcggact ggaaaaagtc cctattaatt gaaacccgtg ctcagaccaa ttgatcaact
    18901 gtttcattcc atctaaaatc gtctgttcat catcgataag aattacgcgt attggcattt
    18961 ttttacccgc cttaagaata gtcttgtaat ttaaaaatac ttatttctag gagaaaatgc
    19021 aaggatattt ttattttaaa gaaacctcac catttcgatc tttggtctag atgctaagat
    19081 tattcctttt gtcatagttg atttgtattt ttttattatt ttaggagaaa tttttgaagt
    19141 atctgaataa taaagaaacc gttttcaaat tttggtatga tgaatgtagc aaacgtaaac
    19201 aactaatgga tggaaaagga gcggataaaa atgaacatca gaaagttaat tgctggagtc
    19261 gtttgtttag cggcagcatc aggtttctta acaggatgcg gaagtgctag tagtacagca
    19321 gacaaagaag aaaaagtcac tgtctgggcg tgggatgaga cattcaatat caaagcagtg
    19381 aatgaagcaa aaaaagttta tgagaacgaa gaaacagaga tagaagttgt tacgatgtct
    19441 caggatgata tcgttcagaa actgaacacc gctttagcga gcgggaatac agaaggattg
    19501 cccaatatcg tcttgattga agattatagg atacaaggct atttgacttc ttatccagat
    19561 gcattcgcta atttgtctgc tattgtaaag gaagatcatt ttgcaccgta taaatttgcg
    19621 gtaaacaaag taggagataa aatatacggt gttcctttcg attcgggcgt aacagcaaac
    19681 ttctatcgta cggatctgat ggcagaagca ggttatacag aagaggatat ggagaatctg
    19741 aagtgggacg agtatattca agtagcgcga gatgtcaaag aaaagacggg aaagaaaatc
    19801 gctgaagtca atccttctga ccttggtcga gtaaggatga ttatgcaaca ggcaggagaa
    19861 tggtatacat ctgaagatgg ggagacagtc acgatccaag acaatgcatc tttaaagtat
    19921 ggtcttgaat tgtttgccac tcttttgaaa gaagatctag tcgaacaaac ctctgactgg
    19981 aatgctggag taactgcgat acaatcagga gcagtagctt ctagcccaac tggcgcttgg
    20041 tattcttcaa cgatccaagg agcagaaaac caatctggaa aatggaagat tgcaccaata
    20101 cctgcacttc cagaaaatct gcaaaaagcg caagcttcta accttggcgg cgctggctgg
    20161 tatgtgatca aaggagtagc tggagaagat tcagcgaaag atttcctaga agcaacgttt
    20221 gcttcgaatg aagaactgat gggcactctt gcgaaagaaa tcggactagt atctacgatg
    20281 cttagcgcga atgaacaaga agcttatcaa gaagcttctg aattttacag tggtcaaaag
    20341 atctttgacg atttttcaac atggacatct gaaataccag aagtgaatta cggacatgag
    20401 acctatgcaa ttgaagcagt cgtagctgaa gctcttcatc gaatcatcaa tggagaagag
    20461 acagacaagg tactagcaga tacacaaaaa caagtagaag cacaattggc taattgatcg
    20521 gtggtaaaaa agtgaatttc ttcgcttttt catcagacaa acgaaaagac aaaaaagatc
    20581 acgtctgcct tacagatatt gtctttcttg tcttttcatg tagataatca atgatagaat
    20641 tggagggaaa agaatgaagc agaaagattt attcaaaaac gggcatgctt ttttaatctt
    20701 gccagtcatt ttgatatcaa tcatggtttt tattccgatg gttttggctt tgatcacgtc
    20761 ttttcaaagt ggtccccctg tcaatatgac atttaacggg ttaggtaatt atcagcggat
    20821 gttgacagat gcaactttta aaaaagcttt ttttaataca ttcctgtatt tacttgtaca
    20881 agtaccgatc atgttgtttc tagcactttt tatttccaac cttttaaatg ataaaaagtt
    20941 gaaatataaa gggttgtttc gaaccgctat ctttcttcca tgtatcactt cgatggtcag
    21001 ttattctctg atcatgaaaa gtctattttc tcaatctggc cttgtaaaca acttgttgtt
    21061 gggcattggt atgattgaca caccgattgg ctggttgact catccaattt gggcgagagt
    21121 tctgatcatt ctttcgatca cttggcgttg gactggatac aatatgattt ttttcctttc
    21181 aggcatgcaa aatattgatt cggagattta tgaagcagca tctattgacg gtgcttctaa
    21241 atggcagcag tttatcaaag tgacgatccc aaatttaaaa ccaatcattt tgtttacttc
    21301 cattacttcg acgatcggga cattgcagct ttttgatgaa gtacaaaata ttactggcgg
    21361 gggaccagca aatgcaacga ccaccctatc tcaatacatc tataatttga gttataaatt
    21421 cacgcctaat tttggatatg cggcagcagt ttcctttgtt attgtttcct ttattgtgat
    21481 attgtcgata atccaaacga aagtaggagg agaaagtcga tgaaaaatat ggtagaaaaa
    21541 gaaacaatcg aacgccccaa cgtcaaaaaa caagcattag aacttcgttc gacaattggg
    21601 tcagtattaa agtatacagc attaagcatc ctatcattta tttccatttt cccttttgtc
    21661 tggatggttt tgggaatgac gaatacaccc gtggatatca catcgggaaa aataaagatt
    21721 ggcagtaact tgatcatcaa ttttcaaaat ttgttttcca atgagctgaa ttttactcgt
    21781 tccctttgga attcagcgat gatcgctttg gtgaccactg tgtttgcatt gctgatttcg
    21841 tcaatggcag gatatggatt tgagatttat caaagtaaac gaaaagacct aattttcaat
    21901 attctactgc tatcgatgat ggttcctttt gcggcattga tgatcccttt atatcgaatg
    21961 ttttcacaat tgaacggaac ggttttggga atcaacagtt tcttggttgt catcctgcca
    22021 tctgttagca ctgctttttt gatcttcttc tttcgacaaa atgttaaatc ttttccaaaa
    22081 gatctagtag aagccgctcg attagacggt ctaaaagaac taagtatttt ttttagagtg
    22141 tatatgccga cggctaaaaa tacctatgcc gcggctgcaa tcatcacatt catgagtagc
    22201 tggaacaatt atttatggcc cttagtagct ttgcaatctc ctgataaacg gacggttccg
    22261 cttgttttat ctgctatggg tgcttcctat acaccagact atggaatgat catggctgga
    22321 ttagtcatcg ctactttacc aactgccatc gtattttttg ttttgcaaaa gcaattcgtt
    22381 caaggaatga tcggctctgt aaaataaaaa aacacaataa gcgcgttcct tcaaagtcat
    22441 ttttccaagt aaaaagatac ttttgggacg tgtgttattt tatgttgaga aaaattaaat
    22501 gataacgttt taagtttgtg ctcgaaaact ctttcatttt aaggtaaact taaagagtaa
    22561 aggaggagga tacatgctaa aaaattttaa agaagtgatt gctgctatat tgccgatgac
    22621 tgtcctgatt gtcattttaa cgtttgtttt tgcaccgtta gaaggagaag acctcgcttc
    22681 atttctggtt ggtgcggcaa tcatgatgat cggaatgacg ctctttttgt ttggagcaga
    22741 ttattctatg atggaagtcg gagatctagt agggaaatat atgatcaaaa agaaaagttt
    22801 gaccgttttg gtcagtttgg gatttgctat cgggatcgtg atcacgattg ctgaaccttc
    22861 cgtgcaagtt ttgggacaac aagtatatga tatatctgac ggagagattg gcagagtgtt
    22921 gctgattggg atcgtcagcg tcggcactgg agtatttcta gcctttgcgc tgcttcgggt
    22981 cgtattcaag ctatcctatt atcaattgat ggctataggc tacataggcg ttttgatcgc
    23041 ctcatttttc accagttccg aatttatgcc gattgctttt gattcggggg gagtgactac
    23101 tggtccagta actgttccat ttatcttggc ccttgccgga gggatgacta gtatgatcaa
    23161 acaaaagaaa aatgaaaatg acagttttgg aatggtcggt attgcttcgt tagggccgat
    23221 tcttgccgtg atgattttgg gggtgatctt ccaatgagtt tctggcagca agtgatagat
    23281 ggaatcgaaa tcgttaccat ggaagtcatt ttggcagtgg cacctatcgt aatcctattt
    23341 gttttaatga atatcattag ttttcgtatg cgcaaaaagc gtttctttga cattatgaaa
    23401 ggtttcttga taacgactat tgggttgatc cttttcctgc acggagtcaa tatggcttat
    23461 gtaccggtag gacagcattt aggcagtgct attgcgggat tgccttacaa ttatatactg
    23521 gttccccttg gctttttaat ggggttctta gtagggtttg ctgagccggc gattcatgta
    23581 atgatcaaac aggttgaaga attgagcgag gggagaatcc gatcgaaaat catgctgatg
    23641 gtaatttcaa ttgggatcgg cgcagcagta ggattgtcta tgtggcgttt gctcgtcgga
    23701 ttctctcttt attatttctt gatcccagga tacgggctag catttatttt aggacgaaaa
    23761 gtggacaaga tctttttagc aatggctttt gataacggtg gagtagcaac gggcccgatg
    23821 tgttcgacat ttattttatc aatgagcgtg gcaatcgctg cacaaattga aggaagagat
    23881 ccgatcattg acgggtttgg tgtggttgca atgattgcat tgacaccaat attatccact
    23941 ttagtactag gatatattta taaacgaaaa gatgaaaaag acagaatcga aaaagaaaga
    24001 ttgcaaaaat tgggggaaag caaatgaaag atcttgtagc attgaatctt cacatgattg
    24061 tgacgatcgt tgacggagga atgggcgaag atgttatcga cttttcaaaa gaagcaggtg
    24121 cagcaggcgg aacgatctta catggaagag gttctggtgt acatgattca gggaaatttt
    24181 taggtttgga catttcgcca gaaaaagata ttgtgttgac attagtaccc gatagtttga
    24241 cgaataaagt aatggatgcg attggtcaag gcatcaacat cgatattcca ggtaatggta
    24301 tttgttttag tattgatgta gacaaagtca ttggtatcac taagattcat caatatcgag
    24361 aaattatcgg aatgcaagaa cttttagatg aaaataacga tagagaaaag tctgaatcat
    24421 agacaagcgt ttgaagagga aataaacaca aacaagaagg cggatgagcg ttatttccgc
    24481 ttttttgttt gtatttttct cgtgaatact tatgaaatag taaattttta tgaaattcta
    24541 ctctaagcat cagactttct tttcaaaata tgctatttta aaaatgatga aaaaaggggg
    24601 agaaaaatga attttttagt tgttttaaaa gatactctga ttgagcgatc tacatttcta
    24661 tatgttcaat tgcaggaaaa taaaacattg ctgcattttt taccagagtt ccatctagag
    24721 gggctaacat taggagaaat ctatcaagca gaagggctgt cggctgtttt gcactttttt
    24781 aaggtagaat tggaattacc agtaaaagat tatatcgagc tttctcttga atcttgggat
    24841 cgagcagtgg ttgaactgtt tccagaaggt ttaatgatcg atacaaacga tgggaagatc
    24901 catctgactg cagcagaact gcaaaaacaa atgcgttatc aaatagatga cgaaaattct
    24961 ttttctattt ttagacgtca gcaaaagatt ctaaaaagct ttttaaaagt gatcagtaaa
    25021 aaacggaatc tgatcaaaac gacacagctg ttgaaaaaat atccaggtct gcttcacacg
    25081 tctattcaat ttcctcaatt gattacgatg atccatcgtt ttatacgtat gcaacagcta
    25141 ccatctaaga agctgtttct tccattgacg gacacatttc gagtagtcaa cagagaagat
    25201 aaaaagaaag tgattgtgat tgactttacc agaaatcgga atgtccttca ccaagttgca
    25261 atggaaaagg ccaattaagg ctttttcttt ttggattttt ttgtcataat aaatattaga
    25321 tgatggagga gataatatga tgcaaacatg gttagaaagg ctaatacgat ttcagcagaa
    25381 ctatcacaaa cttatgcagg gaagatatgc gaaatttgat cagttgaatc gcggtctatt
    25441 gatcgctgcg cttgtcttta gtttgctaag tcgttggttg ccctatagaa tcggacaatt
    25501 gttgttcatc gtctttttcg gatggatgat atttcgtttc ctatcaaaaa aaatctaccc
    25561 cagactaaat gagaatcaac gttatttgaa atatcttgat agagtcaaag aaaaatatca
    25621 tcagataaaa aacaaaaagc aagtcaaaat ggatcgaaaa agttatactt tttttgagtg
    25681 cccgaattgt catcaaaagc agcgtgcacc taaaggaaaa ggacgaatac gtgtgacatg
    25741 caaaacatgt ggtataaaat tcgaaacgaa tgtttaaggg agaaaagaat gaaattagta
    25801 ttattacgtc atggcgaaag cgaagcaaat tttgaaaatt actggacagg atggctagat
    25861 gtcgccttaa cagaaaaagg aaaagaacaa gcagcagttg ccggtcaaaa aatgcttgaa
    25921 gccggacttc aattcgatgc cgtttatacc tctgttttga aacgtgcgat tcttaccgga
    25981 caagtcgcac tagaagaaat gggtcagtta tggcttccta taataaaaac atggcgtcta
    26041 aatgaacggc attatgggga tcttgtgggt aaaaacaaag ataagatgaa agaaatatac
    26101 ggaaaagatc aagtaaaaac atggcgcaga ggatatacgg aagttccgcc gttaaccgca
    26161 gaaaatagat ttgaccgcag atatgatcac ttggatccgc atttgatccc atacggagaa
    26221 agtctggaaa tgacagtgaa tcggatcatt cccttatggc aagatcatct agcaccaaaa
    26281 ctaaaagatc ggcaagattt actagttgtg ggtcatggta atagcatccg agcactgaca
    26341 aaatatttag aagatgttcc tgaagatcaa atggatacga tcgatatccc aaatgcacaa
    26401 ccaatccagt atacattagc agatgatttg acaattctgg acaaaaaaat actttaaaga
    26461 acacctttat ctaaaagtga gagatagaaa aaagaagcaa aggaatggaa caaaaggaac
    26521 atgaaaaatt atatatggga ctttgatggt acattgtttg atacgtatcc cgcaatggta
    26581 gatggtgcat ggcaagcact aaaagatttt ggtatcagta tggacaaaaa agaaatctac
    26641 ttcaaaatga aaaagtattc tactagttat ttgatcaatg aatcaaactt gaatgccaga
    26701 gagtttaatg aattatttca ccgatatgaa aaagaaagta cggaagtttc ccgtccattt
    26761 ccagaaacga aacaagtact ggaaatgcta aaagacaacg gcggtcgtca tttcatcctg
    26821 acccaccgtt tgacagaatc aacatggggc ctgttaaaag aacatcgatt ggcacatctg
    26881 attgaggaag tagtagggat cgatcaggac tttcctcgta aacctgatcc agcatcttta
    26941 aattatttga tcgatacttt tcatttagaa agaacagaca cgatgatgat cggcgatcgt
    27001 cgtttagata tcgaagcagg gaaaaatgca ggagtcgcta cttgtcttta cgacatcgat
    27061 cattttctgg gtgagatccc ggcagattat gtggtaggaa atctgaatga aatccttacc
    27121 ttgcagagca tcattaaaaa gtagtatccc ctttcaaagg agatatgtta taattttcat
    27181 aaaggagtga taaaattata tggaaatcag aagacaaaaa gaagttgtag aagcatttca
    27241 ttatgatatg agattgccga accaagaagt agaaacagat ttgaaagtag gcttctcgcc
    27301 tttaaaagaa acgatcgaaa actatccgaa agaacatagc atcgtaggag cacgcttaga
    27361 gttccgttta gtgtttgata catatgtttt atctggaagt gtgagtcaga tcaatcatat
    27421 cctcaatcgg aaaattgaag gtcaaaatga tgtcacacaa gaagaagtgg acgagctagt
    27481 agcaccgtta ttcgatatcg tacgccgaat gtcttatgag gtgacagaga tcgccttaga
    27541 tcagcctggg gtaaaattga attttcaatc agaaaaacaa gaatagacag taaaaacata
    27601 cctgcgatag attaaagcta tcacaggtat gtttttttat tactaattgt aagcaaaaag
    27661 aatgtcattt cgataaaaat cgtctaaaat ataaaagtca ctgaaaaaaa ttttttcagg
    27721 aggatggttg aagaatcatt ctcgggaacc tttacttcct tcgatggact tgtcattaat
    27781 aatatgagga ggagaaaaat gacaacactc atcacagttt tagtggttct agtttttgcg
    27841 gcattattat atgtgttcta tcgtatgcaa aagcgccata caaaattttc aacacgcgtc
    27901 tttacagcat taggagttgg tatcgtcttt ggcggtctgc ttcaattgat ctttggtgta
    27961 gacgacaaag tcgttactca atcaatggaa tggatcggga ttgtaggtca aggatatgta
    28021 tcattcttgc aaatgctagt tatgccttta gtgtttattt cgatcattgg tgcattcaca
    28081 aaaatgaaag tttctgaaaa gctagggaaa attagtgcga ctgtattgac ttctttactt
    28141 ggaacaacag ctgttgcagc attgatcgga attggttcaa caatgctgtt cggactccaa
    28201 ggagctaaat tcacacaagg agctgcagaa acagcacgaa tctctgaact ggcaacacgt
    28261 caggaaacag ttgaaaactt atcgattcca cagcagattt tatcctttat ccctaaaaac
    28321 gtttttgctg atttagcagg tagtcgtgca acaagtacga ttggagtcgt tattttcgcg
    28381 gcatttgtcg gaatcgctta tctaggcgtg aaaagaaaag cgccaaaaga agcagcattc
    28441 ttttcagatt tagtcgacag tctgtataaa atcattatgc ggatcgtgac gcttgttttg
    28501 cgtttgacac cttatggagt atttgctttg atgacaaacg ttttggcaac aagtgatttt
    28561 gcagctatcg tcaatttagg taaatttgtc gttgcttctt acgtagcatt gcttgctgtt
    28621 ttgattgtcc actcactgat tttgattggt gcaaaagtca atccaatcaa ctatttcaaa
    28681 aaagcctttc cggtattgag ctttgcgttt acttcacgtt caagtgctgg agctttgcca
    28741 ttgaatatcg agactcagac aaaagcttta ggagtagatg atgcaacagc taatttctca
    28801 gcaagttttg gtttgtctat tggacaaaac ggctgtgctg gcgtttatcc agctatgttg
    28861 gcaacgatcg tcgcaccagc agctggagtg aatgtcttta gtttggaatt tattttgatg
    28921 cttgtggcaa ttgtaacaat cagttctttt ggtgtagctg gagtcggtgg gggcgcaaca
    28981 tttgcttcac tgatcgtttt aggggcaatg aacttgccag tagcaatcgt tgggttagtt
    29041 atctctgttg aaccattaat cgatatggca cgtacagcag taaacgtcag cggtagcatg
    29101 gtagctggta tcgtgacaag tgcacgcatc aaagatttag atcgcgatgt catcaatgat
    29161 gattctaaag tgatcgatgt gaatgcatag tcaaaaagag gttgtgatag aagtggtcag
    29221 ctttaagaaa taagaagaag tttcctaaaa atagtttctc aaatttttgt gaaatttcgg
    29281 cttatttccg agaagctact tctgaaacac cgtgaataag aggttgtgaa aaagctgccc
    29341 tgcatcaagt aataagaaca gccaaacaaa aatagttcct catattttac gtttggttga
    29401 gcttattacc gcagatgcaa gcttttgaac accgttttat ttgtctcaca cccttttcct
    29461 tcctacttgt aaccttgctc ccgtgttaga aacatgttac actaaatatg tgaaattatt
    29521 aacaaggagc gtcgttatgc gagaaaaaac agatacactg ccaaaagcaa ctgcgaaaag
    29581 attaccttta tatttacgct acttgaagat gctggatgac tcaggtataa gtagaatcaa
    29641 atcaaatgag ttcagcgaaa tcacccaaat tccttctgca acgatccgca gagacttctc
    29701 gcaattaggc gagctaggca gaagcggata tggctatgat gtcccttttc ttatcgatgt
    29761 attcaataat atccttaata ctaaagaaga aaaacgaatc gcattagtag gatatggaaa
    29821 tcttggcaaa gcattgaaac acaacaattt tagaagaaat gaaaatctaa atatcgtgtg
    29881 cgtctttgat aatgatccag cactgatcaa tcgagtaatt gatggagaaa tgatctatcc
    29941 gattgatcgt ttcgcggaga tagccaaagc aaaaaatgtg acagtagcaa tctctacggt
    30001 accaagtaaa tattcacaat ctgctattga tgaaattgtc aaaggaaatg taaccgctat
    30061 acttaatttt gcgcctgaca gagtaactgt cccagcttat gtcaatgttc aatacattga
    30121 tttgacaacc gagttgcaga cgcttattta ctttgatgag aattactcag aagttttttc
    30181 ttaggaaaat ttgagtaggc ttattgatct tgcgagaact cgtcgtaatt ctttcaaaaa
    30241 ttttttagta ctaaatgaag tggttttttc acgaccactt gttgactaaa aaaaaaaacc
    30301 attgtagaat gaagcataac gctgaacaag aggagtaact ggcacattca ttcttagaga
    30361 gagaactatc tgctgaaagg ttctcaatga caaccagtga aggtagcttg ggagtttctg
    30421 tcttgaatgg agtagagata gacgagtcgt gatcgttaac tcacttgaga ggcaataatg
    30481 attgattatt gcaatttagg tggtaccgcg taaatcatac gtcctagaat acgaaagtat
    30541 cttaggacgt tttttagttt aaaggaggaa aacagcatga cggaagaaaa taacctatca
    30601 acaaaatata atccgcaaga agttgaagct ggacgttatc agcattggtt agaacaagat
    30661 ttgtttaaac cgagcggaga taaaaaagca aaaccttatt caatcgtaat cccgccccca
    30721 aatgttactg gaaaacttca tttagggcat gcttgggata cgaccttgca agatatgctg
    30781 attcgtcaaa agagaatgca aggatatgat actttatggc tgcctggtat ggaccatgca
    30841 ggaatcgcga cacaagcaaa agtagaagcg aagcttagag agcaagggat ttctcgttat
    30901 gatctaggtc gtgaaaagtt catcgatcaa gtgtgggaat ggaaagaaga gtatgcgagc
    30961 cacatccgcg aacagtgggc taaaatgggc ttatctttag attacagtag ggaacgtttt
    31021 acattagatg atggattatc tgaagcagta cgtaaagttt ttgttacctt atacgaaaaa
    31081 ggtttgattt atcgtggtga atatatcatc aactgggatc ctcaagcacg aacagctcta
    31141 tctgatatcg aagttattca taaagatatc gaaggcgcct tttatcatat gagctatgaa
    31201 ctagcagatg gatcaggcgt agtagaaatt gcgacaaccc gtccagaaac gatgttggga
    31261 gatacggcga ttgctgttca tccagaagac gagcgttatc aagcgctcat cggcaaaaaa
    31321 gtgattctac ctttagtcaa taaacaaatc ccaatcattg cagatgaata tgtagatatg
    31381 gaattcggga caggtgtagt aaaaatcact ccagcacatg atccaaacga ctttgaggta
    31441 ggtaatcgtc atgatcttcc acgcgtaaat gttatggatg atgatggtac gatgaatgaa
    31501 ctagcaggaa aatatgctgg aatggaacgt tttgctgctc gtaaagcaat cgtgcaagat
    31561 ctgaaagaaa tcggtcgatt gattaaaatc gatacgatga tccacagtgt gggtcattcc
    31621 gagcggacag gtgtcgttgt tgaacctcgg ctgtctactc aatggtttgt aaaaatggcg
    31681 ccgttagcga aaaaagccat tgataaccaa gaaacagatc aagcagtgga attcttccct
    31741 ccaagattca atcaaacgtt tttacgttgg atggaaaatg tccatgattg ggtaatctca
    31801 cgacaattat ggtggggaca tcaaattcct gcatggtatc acaaagaaac aggtgaaatg
    31861 tatgtaggga tggaagcacc agcggatagt gaaaactggg tacaagatcc tgatgtttta
    31921 gacacttggt ttagttcagc tttgtggcct ttctcaacaa tgggctggcc agatacggaa
    31981 agcgaagatt ataagcgata cttccctact agcactttag taaccggtta tgacatcatt
    32041 ttcttctggg taagcagaat gattttccaa agcttggaat ttactggcga acgtccattt
    32101 gagaatgtct tgattcatgg attgattcgt gacgaacaag ggcggaagat gagtaaatca
    32161 ttaggtaatg gaatcgatcc gatggaagta atcgaaaagt atggagcaga tgctctccgc
    32221 tggttcttat cgaatggttc tgcaccagga caagatgtcc gcttcagcta tgaaaaaatg
    32281 gatgcttctt ggaactttat caacaaaatc tggaatgctt cacgtttcgt tatcatgaat
    32341 atcgaaggaa tgaccgtaga agagatcgat ttatctggag aaaaatcagt ggctgaccgg
    32401 tggatcctta ctcgtttgaa tgaaacgatc gaacgagtaa ctgagctatt tgatcgtttt
    32461 gaatttggtg aagcaggtcg acaactgtat aactttatct gggatgattt ttgtgactgg
    32521 tacatcgaaa tgagtaaaga agtactttat ggagatgatg ctgtttcaaa acaaaccacc
    32581 cgtagcatct tagtccatac acttgatcag atcctacgtt tattgcatcc aatcatgcca
    32641 ttcgttacag aagaaatatg ggaacatatt cctcatcagg gaaattctct agtagtggca
    32701 gaatatcctg ttgtacgtcc agagtttgat gatgaaacag cttctaaagg gatggaagta
    32761 ttgaaagaat tgattcgagc cgttcgaaat attcgttcag aagtgaatac accattgtct
    32821 aaaccaatta cgttgctaat taaaacaaac gatgctaaaa tcgatcaatt tttagttgaa
    32881 aatacaagct atatcgaacg cttctgtaat cccgaagagt tgacgatatc aagtgagatc
    32941 actgcgccag atctagcgat gtcagctgtt ctgacaggtg cagaaatctt cttgccgtta
    33001 gcaggattga tcaatattga agaagaaatc aaacgattag aaaaagaact ggaaaagtgg
    33061 acaagcgaag taaaacgtgt ccaaggaaaa ctttctaatg aacgttttgt ttctaacgcg
    33121 ccggaagaag tagtagaggc agagcgtgca aaagaaaaag attatttaga gaagcaaacg
    33181 gctgtcaaag aacgaatcga aagtttgcgt actattcaat aagaatagaa aaagagagtg
    33241 tgaaatcagc tagctattac taaaagctag ctgattgaac atcgttacag gaaagagttt
    33301 gagcctcact cttagcttcg attcataagg agatgtgaca taaattttgt cgcatctctt
    33361 ttttagtcta ctcttattat gtaggagaag gagggaggtg gatcatgtat caagagtcaa
    33421 taaagtagtt gataaaatcg ttcctctctc tgtttctttt ctctttgtca tacttttatt
    33481 tttcttatgt gttctatttg ttataataaa agagaaatgg gggaattgtt gatgacgatt
    33541 gaagaagcaa tagaatggat ccatagtcga ttaccatttg gttcacgtcc tggccttgac
    33601 cgagtcgaag ctttattaga agtggtaggg catccagaga gaaaagtaaa aacgatccat
    33661 atcgctggaa caaacggaaa aggttctact gtagcttatt tgagaagcct attagaagag
    33721 gctgggctga cagtaggtac atttacttca ccttatatcg aaacgttcaa tgaacggatc
    33781 gcaatcaatg gcaaaagcat tccagacaat gatttgatcc agcttgtaaa aaaatatcag
    33841 ccactgatca aacaacttga tcaatttgaa gcagtagctg gtattactga attcgagaca
    33901 ttaacagcaa tggcatttga ttattttttg atgaagaaag tggacgtagc gattatcgaa
    33961 gttggcttag gcggcttatt ggattctacc aatgtagctg taccaatgct gacgggaata
    34021 acaacgattg gacttgatca taccgaaata cttggagata caattgaaga aatcgctgca
    34081 caaaaagctg gaatcatcaa acaaggcgtt ccagtagtaa caggaaatat tgccccagaa
    34141 gcttttactg tcatccaaca aacagctaaa gcaaaaaatg caccgcttta tgtatacggg
    34201 gaaaattata aaataaacta tctgcatcct gatgagatgt ggggagaagt atttgaattt
    34261 tttaatgaaa taggaaagat ccctcaatta accactccgc ttttagggca acatcaagct
    34321 gaaaatgcag gagtagccat cgaattgtat tttgtttact gtcaattgga aaagctgcct
    34381 ttccgagaaa aagatgtttt gaagggattg aagaaatctc gatggccagg aagaatggag
    34441 cgtttgagta acgagccatt agttgtttta gatggtgccc ataatacgca tgcaatgaac
    34501 cggcttgttg agaatttgaa aaaagaattc aaaggatatc aaatcaacat tttgttttcc
    34561 gccttacgaa ctaaaaatgt tagagaaatg ctagaacgat tgcagcaagt accaaatgtc
    34621 catatttatt tgacgacctt tgaacatcca aaagccatcg aattaagtga agtatcagat
    34681 ttagcaagtg agagagtatc aatcgtttca ttgtggcaat tcggattagc agatatattg
    34741 gaaaagatga caaatgaaga cgtattagtt gtgaccggtt cactttattt catttcagaa
    34801 gttcgccatt tattaataca attaggagga gatcatgaag tataatgcag tgatttttga
    34861 catggatggt ttgctgttcg acacagagtt agtgtattac gaagccacac aagtagtagc
    34921 agacaagatg ggttttcctt atgataaaga cctgtattta aaattcttag gtgtttcaga
    34981 tgaagaagta tgggcgaatt atcatatgat tttctcggaa tttggcaaaa caagtgtaca
    35041 aagattcata gatgattcat accaggaaac gcttgaacgt ttttcgcaag gagcggtaca
    35101 aatgaagccc ggtgtgttgg aactcctctc ttttttagaa gaacatcaga ttcccaaagt
    35161 tgtcgcttcc agtaaccaac gagcagtcat tgaactttta ttgaagaaga acaatctgtt
    35221 tgaacgattt gagacgattg tttctgcaga aaatgttcaa cgagcaaaac cagacccaga
    35281 aattttcttg ttagctaatc agaagctagg tacagcgaag aaagaaacat tgattttaga
    35341 agattcacaa aatggtattt tagcagcttc tgcggctgag atcccggtag tcatggtacc
    35401 agatctgctt ccaccatccc aagaattgat caaaaaaaca gctggggttg tttcttcttt
    35461 acatgaaatc cctgcattgt taaaataaaa atccatctct tttcgtatct tattttagat
    35521 aagatagaaa ggagatgttt ttttgggaaa acgattgatc aaagaagtac ctactagctc
    35581 tttaccaagg gaacgtatgg aaatttatgg cgcggaagct ttatcggatc aggagctttt
    35641 ggcaatctta ctgcgtaccg gtcaacaccc ttatagtgtg atgtcgattg ctggaaatct
    35701 gttaaaaacg tttggaggac tggcctcttt acgacaggct actttgcatg agttagaaga
    35761 gattcgaggg ataggacgcg tgaaagcaat cgaaatcaaa gccttgatcg aattaggtcg
    35821 acgaattcag acggatcatg aaagagcggc tccaccggtt cgtaccagct acgaattggc
    35881 ccagcaattg attctggaaa tgaaagatca caaacaagaa catttggttt gtatttattt
    35941 ggacacgaag aatcaagtga tcttaaaaaa aacagtgttc atcggttcgc tgaatcagtc
    36001 agtcgctcat cctagagaaa ttttccatta tgctgtacgt tattgtgcag caaggatcgt
    36061 tttagcgcat aatcatcctt ctggtaatgt tattccatcg cagcaggata tgaattttac
    36121 caaaaggata caaaagtgtg gagaaatgat ggggatcact gtactggacc acttgatcat
    36181 cggacgcaaa cgatatttta gtttgagaga agaaggaatg atggaagaga agtaagttgc
    36241 tttcttgcaa aaaaaatgaa gcaagtgtac aattatattg taattttgtt tgggaataga
    36301 agttgaaaca tctgttgcac ctctatcgta cacgagcaat gttacaattt aaattagtgc
    36361 ttttggcacg aggaggatat tattatggaa aacggaacag taaaatggtt caacgctgaa
    36421 aaaggatttg gatttatctc acgtgaagat ggaagcgacg tattcgttca cttctcagct
    36481 atccaaggtg acggattcaa aacattagaa gaaggtcaag cagtgacttt cgacgttgaa
    36541 gaatcagacc gcggcccaca agctgcaaac gttgtaaaag cttaatttta tctgtaaatc
    36601 aaattgatgg tagacatcaa tttttacgag agaacaaagc gagtaacaaa aatacctgcc
    36661 acaagagaca atttctcttg tggcaggtat ttttttgtat gaaaaagtaa ataaatttaa
    36721 gaggctatga aagagccacc ttgtatcaag gaataagaac aaacaaaaat ggcttctcat
    36781 gtttttcatt tgttttctct tatattcagg acaaggtgct gatttcataa cctcttattt
    36841 attcttcatc tggtgtcatt accattggca aaatcattgg atgtctttct gtacgttcga
    36901 ataagaaagg ctgaagcgcg ccagtcatcg cttctttcaa tttatattct gaacagtcag
    36961 gtccattcaa acattctctt aaagcgtgga ataagattcg ctgagcttca ttgatcattt
    37021 ctcctgattc tcgcatgtag acaaatccac gagagagaat atctggacca gccaagattt
    37081 cttttttctt gatatccact gtcgcgacag ctaatacaag accttcttct gaaagcaggt
    37141 gacggtcacg taaaacgaca ttaccaatat caccgatccc gctaccatcg atatacacat
    37201 ctccagccac aaaatgccca gcaggacgcg cagaatcgct agttaacgct aaaacatcgc
    37261 cattttccat gatgaagcag ttttctgcag gaacacctac atcttgtgct agagaagcat
    37321 gtactttcaa catgcggaat tctccgtgga caggaataaa atattttggc ttcatcaaac
    37381 gaagcattaa tttttgttct tcttgtccac cgtgaccgga agtatggatg ttattgattt
    37441 ttccgtgaat cacttctgca ccagcttcag acagcaaatt gatcaaatga ttgacacttg
    37501 ttgtattacc aggaataggt gaacttgaaa agatcactgt atcgtctggc tggatttgga
    37561 tttgacggtg agtaccattc gcgatgcggc tcaacgcggc catcgcttct ccttgagaac
    37621 ctgtacatag gatcatcgtt tcattggcag gcaagcgatt gatttcgtgt gcatctacaa
    37681 atgtgccgtc tggaactttg atatatccta aacgttgacc gttgacgatg gcattttcca
    37741 tgcttcgtcc gaaaacagcg attttacgac cagttgccac agcggcatct gctgcttgct
    37801 gcaaacggaa gatgttagaa gcaaagctgg caaaaatgat acgtccttcg atttttgaaa
    37861 agatcttcaa aatagaatgc ccaatcgttt tttctgattt agtaaatgtt ggaatctcag
    37921 catttgtact atcagataaa aggcacaaga ctccttcttc cccgatacga gccattttat
    37981 gcaagtttgc tggttcgcca acaggagtga aatcgaattt gaagtctcca gtcgctacga
    38041 tatttccaga aggtgttttc accacgatcc ctaatgcatc tgggatacta tgggtcgttc
    38101 tgaaaaagct gacagaagtc tttctaaaac ggatgatcgt atcttcattg atctcatgga
    38161 gctctgcatc tcgaagcaag ccatgctcat ctaatttgtt tgtgatcaac gctaatgcga
    38221 gaggaccagc atagatagga atatttgctt gtttcagcaa gtagggtatt ccgccgatgt
    38281 ggtcttcgtg cccgtgagta atcaccaagg cttttacttt gtgtaagttt tgtatgatgt
    38341 agctgtagtc gggaatgaca tagtcaattc caagtaaatc gtcttccggg aatttgatcc
    38401 cggcatcaat caaaatgatt tcatcttgga attgtacccc atacgtattt ttcccgattt
    38461 cacctaatcc accaatagca aagacgcctg tctcgttatt tttgatggaa agtttcatat
    38521 taaaactcca ctaatgagaa atcagcattt tctttttcgt actctagatg acttccttct
    38581 agaagttgaa cgaattcaat attgtatggg gtattttctt ccacacgttg gcgagcgtct
    38641 acatcgcttt ttgcctcaat gtaaagagat tttgtttctt cccgtttagg gttgcgtact
    38701 tttgtttctt gataataaac tttatagatc attgttttgt ctcctttatc tgcgtaaagc
    38761 agtgatttag ctagttgatt tttttagaca acacaaaacg aggggacaag ccacctggtt
    38821 tttcatatgc tttgactaga atactggatt cgagtcaaag agacaagcct cgtttttgct
    38881 tatctgttcg gatatttttt caaccgtacc ttctgtataa aaaatataca acaattaatt
    38941 gttattctag cataaaacag caaataagta tagaaatcaa ctagattgaa agaaaaaatc
    39001 aatgtttatc atcccgctat aatcataatg acttttttat gagagaagtg ttctattaat
    39061 aggctcgttg tgctatcatt tttagtaatg aaaatgtaac gaaagattaa aaggagatga
    39121 gaaagtgaaa ctaatgtggc attataccat gcgttataag aaattcttgt ttttcaattt
    39181 catatgcgta tttggtttta ttttgattga attaggactt cctacgattt tagcacgtat
    39241 gatcgatgta ggaatccgca atgatgattt tgattatgtg aagcaacaag ggatatttat
    39301 gatcgttatt actgtaatcg gtattatttt gaatatcttg ttaggttatt ttggttcgag
    39361 gattacgact aatatcgtag cagatattcg agatgatttg ttcgaaaaga tccaaagtta
    39421 ttcgcatcag gaatacgaaa atctaggcgt atcttcattg atcactcgta caacaaacga
    39481 tgcttatcag atcatgttgt tcctgcaaaa tattttacgt atcggtttta tggcaccaat
    39541 gatgttcatt gtcagtttgt acatggtcat gcgtacaagt gtgacactca gtttgtatgt
    39601 agtaggtgca ttacctttac tattattggc agtcgtagcg attgctaagg tgtctgaacc
    39661 gttatctaaa aagcaacaaa aaaacttaga taaaatcaac agtatcttgc gggaaaatct
    39721 ttcaggattg cgtgtgattc gtgcattcgt caatgaaaag tttgaagaaa aacgttttgc
    39781 tgtagtcaat gagaattatg cttctagttc aaaaagctta ttccgtttga tggctgttgc
    39841 acaaccgggt ttttatttct tatttaatat cgtaatggta ttgatcatct ggagcggggc
    39901 agtgcaaatc gatgcaggcg agcttcgtgt aggagatttg attgcgttta tcgagtatat
    39961 attccatgcc ttgttctcat tcatgttatt tgctagtgtg ttcatgatgt atccaagagc
    40021 agctgtttca gctggacgta tccaagaagc cctagatgcc aaaccttcga ttgtagaaga
    40081 tccaaatggt atatctgata caaaaactaa agggtatatc gaatttcaaa atgtaacatt
    40141 tgcttatcca ggccattcgc aagagccagt gatacgaaat gtcagtttta cagcctctcc
    40201 aggagagacc gttgctttta ttggaagtac cggtagcgga aaatctacgt tgatccaatt
    40261 gatccctcgc ttttatgatg tgagtgaagg aaagatcttg atcgatggag tagacgtgag
    40321 agattatcgg ctaagcaagt tgcgagataa gattgggttt attccgcaaa aagcgctgct
    40381 atttactgga acgattgcag ataatttgcg atacgggaaa gaagatgcta cgcaagaaga
    40441 aatggaacgt gcagctgaga ttgctcaagc ttcagaattc atttctcgaa aaccagatgg
    40501 atttaatgaa catttatcag aaggtggttc caatttttca ggtggacaaa aacaacgatt
    40561 ggcaatagcc agagccatca tacgccgtcc ggaaatctat atttttgatg acagcttttc
    40621 tgcattagat tatcaaacgg atgcgaagtt acgtgctcgt ctgaaaaaag aaacgacaga
    40681 atctactgta ttgatcgtag cacaacgtgt gggaacgatc atgcatgcag ataagatcat
    40741 cgtattaaat gaaggagaag tcgtcggtat ggggacgcat cgggagttgt tggaaaaatg
    40801 cccgatttat tatgatattg ccgcttctca attatcaaag gaggaattag catgaaaaaa
    40861 accttttctt ccttaaaacg tttagctcga tatatcagac cgtatcgtga gacttttctt
    40921 ttagtgatcc tatttacagt tctgacagtg gctttcaacg tggctttgcc atatgttaca
    40981 ggacttccta ctacagaaat cagccgtaat attgccaatg gcgaatcaat caatttttct
    41041 tatatattca aatgtctcat ttggatcaca gttgttggaa tagggtattg tatttcgcaa
    41101 ttactttctg gtgttttgat gacaaatgtc gttcaaagtg ccatgcatga tttgagaaga
    41161 gacatagaag aaaaaatcaa tcgtcttccg gtttcatatt tcgataaaaa ccagcaagga
    41221 aatattttgt ctcgtgtgac gaatgatgta gatgcagtca gcggtgcttt acaacaagct
    41281 tttattggta tcgtaaatgc gatattggga attacgttgg ctgcagcaat gatgttttat
    41341 atccagccga tgatggcgct gatctctatg atcatgattc caacatcgat ctggatctca
    41401 aaacgagtga tcaatgcttc gcagaaatat tttcaaagca tgcagaattc tcttggtgag
    41461 ctaaacggct acgtccaaga aaatatgaca ggttttagtg tattgaaagt atatggacgc
    41521 gaaaaagaaa cattggaagg attcaaaaaa gttaatcaca gcttgaaata ctttgggttt
    41581 cgtgcagcat tcatttctgg cttgatgatg cctctagttc aattaactgc ctatgctact
    41641 tatattggta tggctgttct aggaagcttt tatgttatta ctggggcgat cgttgtcggt
    41701 caactacaag cattcatcca atatatttgg caaatcagtc agccaatggg gaacgtgaca
    41761 caattgtctt cagttttgca aagtgcatca gcggcaacga aacgtgtatt cgagatttta
    41821 gatgagccag aagaaaaaga aaacaaagta gatgcttcac tgccagaaac gattttagga
    41881 gatgtgacat tcgatcatgt cgattttgca tatgatccga agaaaccatt aatccaagat
    41941 ttgaattttg aagtgaaagc tggtcaaacc gtagctgtgg tcggtccgac cggtgccggc
    42001 aagacgacat tgatcaatct attgatgcgt ttttatgacg tagacaaagg agcaataagg
    42061 attgatggaa tcgacaccaa atcaatgagt cgcagtgatg tgcgttcgct atttggtatg
    42121 gtcttgcaag atgcatggct ttatcaagga acgattgcag ataatatccg cttcggaaag
    42181 cttgatgcga cagattatga agtagttgat gctgcgaaaa cagcaaacgt agatcatttt
    42241 atccgaacga tgccagatgg atatgatatg gccatcaatt ctgagggaga caatgtgtct
    42301 ttaggacaaa aacagttgct gacgattgcc cgagcagtgg tctccgatcc taaaatcttg
    42361 attttagatg aagcaacaag ttcagtagac acccgtttag aagcgttgat tcaaaaagca
    42421 atggatcgcg tgatggaggg acggacaagt ttcgtcatcg ctcaccgttt atcgacaatc
    42481 cgtgatgcag atctgattct agtgatggat caaggtcaga tcattgaaaa aggaacgcat
    42541 gagagtttat tggcacaagg tggattttat gaaaaactgt ataacagcca atttgctgag
    42601 gaagcagaat aagtcatata agaataaaac agtttaaaac ttattttttg ttctctattc
    42661 ttgataatgg taaagtaaag ttatcaagaa aagtgaggac acggagatgg aacagttttt
    42721 aaatgaaaaa attactgaag ccaaaagaga agttgatcgt ttaacgcacg tctgtcagac
    42781 gaagcctttt gattcagctg atttttttga aaatgagttt caaatcgaac gcttggcagc
    42841 acaaatagaa gtgtacgaat taattttaaa aaaagtaata gaagaaaata tagatgtaca
    42901 ataaaaacag gtaaaacgtc agttaccaga tactgtaaat agacatctgg cctgagcagg
    42961 ttttacctgt tttttttctt atgaaataaa tatatgctat gataccttta ttcaatagga
    43021 ttagtttcta caaaaaatga aaggagaaaa ataaatatgg aacggtttat agcatcaaat
    43081 gccacagtga ttggtgatgt gacattgtca gaagatgtga cgatctggta tcaagctgtt
    43141 ttacgcgggg acagtaattg gataaaaata ggacaaagaa cgaatatcca agatggtaca
    43201 atcatccatg tcgatcatga tgcacctgta gatattgcgg agaatgtgac ggtaggacat
    43261 cagtgcatgc tacatggctg tacaatcgaa aaaggggcgc tgatcgggat ggggaccacg
    43321 atattgaatc atgcggttat tggtgagaat agtttgattg gtgctggatc attagtaaca
    43381 gaaggaaaag ttattcctcc taatgtcttg gcttttggtc gcccagctaa agtgatccgt
    43441 cctttgacaa aagaagaaat acaaaaaaac aaagaaaaca tccagcatta tgtcgagata
    43501 ggtcggatgc atgcgcaagg aaaattcaag gagcaaagat aggcagtagc atccataaaa
    43561 taaaaaagga tggtcagttt gagttgacca tcctttttta tttggtttag ataagagagc
    43621 ttaaaaaagc ttcctttgaa acagattaat ttaatgaaga actgttttcc atgacttcat
    43681 cgatcaatcc atatgctttt gcttgttcag ctgtcatata gttgtcacga tctgtatctt
    43741 tttcaatcac ttcaagcggt tgtccagtgc gttcagctaa aattttgttt aagcgttcgc
    43801 gtgtttgcaa aatatggcgt gccgcaattt cgatttcagt tgcttgccct tgagcgccgc
    43861 caagaggttg atggatcatg atctcagcat ttggtaaagc aaatcgttta ccttttgtac
    43921 cagctgtcag caagaagctt cccattgaag cagccatacc cataacaatt gtttggacat
    43981 ctgctttgac aaagttcatt gtgtcgtaaa tagccatacc tgctgtaact gaaccaccag
    44041 gagaattgat ataaagatag atatcttttt cagaatcttg agcgtctaag aaaagcaatt
    44101 gggcgatgat cgaattagca agatcatctg ttacttggcc gcttaacatg atgatacggt
    44161 ctttcaataa acgtgagtag atatcgtaag ctctttcacc tcgtgaagat tgttcaatga
    44221 ctgtaggaat taaattcata atagagttcc tccttgattt cgtttagaaa tagtgtacca
    44281 taatttagtt tctaatacct aaaatgtagt atacattttt ggtcaaaaaa ggtcaaataa
    44341 aaaacttagg caaattagtc gcataaaccc gctttatggt atgataagca gagaaacaaa
    44401 acaaggagga acaacagatt gatagttggt attactggcc ttatcggagt gctgacaggt
    44461 gctgtagttg tctatatcgg catgatactg caaatgcgtc acttggaaaa taaagaacag
    44521 cgtagacgtg aattggaatt gaaagtcaaa gaaatcgaga cgctgaatca attaaataaa
    44581 aagatcaacg agatcttgca aaaacggaca acgctgatgg aagaatatgt cagctttgat
    44641 gcatttgatg attgttatat cacgattgac gattttgcgt atctccaatc attttcagcg
    44701 caaaatagtt tttatctgcc aaattatatt ttagacgagc tatttaagaa tatctcccat
    44761 cgaaaagtga ttttatctcc agatgaaaca atgaagattg gtggatatgc ttataaaggc
    44821 ggacgcgtga ttttagagaa ttttacggat agtctgactg aaatgatcaa tgaaaaaaaa
    44881 gctcagttga agactatgac gcgccagccg attggtttct tctcgaaaga tgatgtttgg
    44941 agctgatatt ttgaagcatt gccgttgcac atgagagcgt ggtaatgctt tctttttttc
    45001 ttaatgatac aatagaactg aacggagtga agaaagatgt tacaaatcta tgcccatcgc
    45061 ggaagcagcg gcacccaccc agaaaatacg cttcctgcat ttgcagaagc tgtacgagta
    45121 ggtgcagatg gtatcgaact agatgtccat ctaagcaaag atggttattt gatcgttatg
    45181 cacgacgaag aagtggaccg gacgactaac ggtaaagggc tgattcgaga aaaaacattg
    45241 gaagaactca aaaaattgaa tgcagggggc aaattcagca aagaatttcc agcagctaaa
    45301 gttcctgaat tgagagaagt gttaggactg ctcctccaaa aaaattaccg agggatgcta
    45361 atgattgaaa ttaaaactga tcagtatgaa tatcccggta tcgaagaaaa attagccgaa
    45421 acattaaaaa gtagagattg gccttttcaa catgcatatt gcagctttca tttggattct
    45481 ttggaaaaaa tcgcattact ggaaccggag atacagctgg acttattgat gagtacttct
    45541 gataaaaaga tccaactagc agcagaaacc ccttatatag aaggagtaca tccaaaaaat
    45601 acttggttgt tcgaacatcc agtccaggca gctgattttc caaaagcaat tcgtgcatgg
    45661 acgattaacg atgaagaaga aatggccaaa tgtcaagaac tcaaaataga tggaataata
    45721 actgattttc cggagaaagc tcttcggtgg aaacaatcaa cgaaaagtag gtaaaatatg
    45781 gaaaaagtat tagtgatcgt cggtccgaca gcagtcggta aaacagcgct aagtgtggaa
    45841 ctcgcacaac tatttggagg agagatcatc agtggtgatt cattgcaaat ctataaaaat
    45901 ctggatatcg gtacagcaaa aatcaaagaa gaagagatga ataatgttcc gcatcatttg
    45961 attgatgtga tcgaacctac tcaacgttac tcagccgctg attttcagca ttcaggtcga
    46021 caattgatta ccgagattgc gaaccgaggg cacctaccta tcatagctgg tggtacgggt
    46081 ctctatatcc aatcccttct atatgattat cagttaggag cgacagaaga agcgaatcct
    46141 gagattagaa aaaagtatga aacgttagct gaaacaattg gttcccaagc attatggaat
    46201 cagttgaaag aaaaagaccc ggcagctgct gagaaaatcc actggaacaa tcaacgcaaa
    46261 gtgataagag ctctggaagt tttagagacg acaggacata gtatcacggc acctaaagaa
    46321 gagccaaagc aattgtatga ttacctcatg attggattag atacgaagcg tagtattctt
    46381 tatgaacgaa tcaatcaaag agtcgatttg atgctagcag aaggtttagt agcagaagcg
    46441 cgtaaggtgt atgagcttgg agaagtccag gccagtcaag gtattggata taaagaattt
    46501 ttcccttatt ttgaagggaa agaaacattg gaagaagcaa cagaacaagt caaactacat
    46561 tcgagaagat acgcgaaacg tcagttgacg tggtttcgta accgtttgga tgcacagtgg
    46621 tttgatctta taagtgagcc aaatcaaaaa agtcagattg agcaaacaat caaaaaatgg
    46681 ctggaggcgg aataaaatgg aagaaaaagt gatcatcgta ggggtagaaa ccgaagcaaa
    46741 ccagcgttac ttttcagagt caatggaaga gctagttcag ctgacgaata ccgcatcagg
    46801 agaagtcgta ttcacaatta cacaaaagag accacaagtg gatcggcaaa cgattatcgg
    46861 taaagggaaa ttacaagaat tagttcaaca agcggatgcg catgaagcgg atttgatcat
    46921 ctttaatcat gagctaactc cgagacagag ccaactgatt accgatgctg ttggtcttcc
    46981 ggtaattgat cgcgtgcagc ttatcttaga tatttttgcg atgcgtgctc gttcgaaaga
    47041 agggaagctg caggttgaac ttgctcagtt agaatacctg ctgcctcgtt tagtagggca
    47101 agggaaaaca ttatctcgtc ttggcggtgg gatcggaaca agagggccag gtgaaacaaa
    47161 gttagaaaca gaccgccgac atatacggaa taaaattttt gctgttaaga aagaattgaa
    47221 agaagtagaa gctcatcgag agcgcaatcg ccaaaaaaga aaaaatagcg agatttttca
    47281 gattggactt attggctaca cgaatgccgg gaagtcaact atcttgaatc tattgacaca
    47341 agcggatact tattcgaaag atcaattgtt tgcaacgtta gatccattga ctaagagatg
    47401 gcgttttgct gaaggatttg agattactgt aacggatacc gtcgggttca tccaagacct
    47461 gcctacgcaa ctgatcgatg ctttccattc tacattagaa gaaagtcaaa atatggatct
    47521 tttgctacat gtagtcgatg ctagttctcc agatcgtatt ttacaggagc agactgtctt
    47581 aaaattgatg gatgaactga atatgaaaga aatgccgata ctgactgttt acaataaggc
    47641 agaccagatc gacccagcga tgttcacgcc tacgctattc ccaaatgtat tgatttctac
    47701 tcagacgaaa gaaggtaaga tggcgttgat cgaagcaatc aaacgtcagc tgatggaact
    47761 aatgacccct tatacacttt ctgtgccaag caacgaagga cagaaattaa gcgagttgag
    47821 aagacaaaca atggttttgg acgaagcgta catggaagaa acaaacgaat atgaagttaa
    47881 agggtttgca aaaaccaatt caaaatggtt aagaaaaaac caataaaatt ttattttttt
    47941 aa
//
LOCUS       DACECY010000019        45133 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.020, whole genome shotgun sequence.
ACCESSION   DACECY010000019 DACECY010000000
VERSION     DACECY010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.020"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            536..1069
                     /locus_tag="IWO38_001081"
     CDS             536..1069
                     /locus_tag="IWO38_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ0313182.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(1559..2083)
                     /locus_tag="IWO38_001082"
                     /pseudo
     CDS             complement(1559..2083)
                     /locus_tag="IWO38_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(2076..2549)
                     /gene="tsaE"
                     /locus_tag="IWO38_001083"
     CDS             complement(2076..2549)
                     /gene="tsaE"
                     /locus_tag="IWO38_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ0313183.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(2672..3652)
                     /gene="pta"
                     /locus_tag="IWO38_001084"
     CDS             complement(2672..3652)
                     /gene="pta"
                     /locus_tag="IWO38_001084"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ0313184.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(3805..4485)
                     /locus_tag="IWO38_001085"
     CDS             complement(3805..4485)
                     /locus_tag="IWO38_001085"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ0313185.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            4611..5480
                     /locus_tag="IWO38_001086"
     CDS             4611..5480
                     /locus_tag="IWO38_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0313186.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            5506..6021
                     /locus_tag="IWO38_001087"
     CDS             5506..6021
                     /locus_tag="IWO38_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313187.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(6068..6631)
                     /locus_tag="IWO38_001088"
     CDS             complement(6068..6631)
                     /locus_tag="IWO38_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0313188.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(6686..6865)
                     /locus_tag="IWO38_001089"
     CDS             complement(6686..6865)
                     /locus_tag="IWO38_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313189.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(6942..7451)
                     /locus_tag="IWO38_001090"
     CDS             complement(6942..7451)
                     /locus_tag="IWO38_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313190.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(7547..7927)
                     /locus_tag="IWO38_001091"
     CDS             complement(7547..7927)
                     /locus_tag="IWO38_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0313191.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            8257..9006
                     /locus_tag="IWO38_001092"
     CDS             8257..9006
                     /locus_tag="IWO38_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ0313192.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(9190..10467)
                     /locus_tag="IWO38_001093"
     CDS             complement(9190..10467)
                     /locus_tag="IWO38_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313193.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10546..11190)
                     /gene="pcp"
                     /locus_tag="IWO38_001094"
     CDS             complement(10546..11190)
                     /gene="pcp"
                     /locus_tag="IWO38_001094"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ0313194.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(11389..12333)
                     /locus_tag="IWO38_001095"
     CDS             complement(11389..12333)
                     /locus_tag="IWO38_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ0313195.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(12335..13021)
                     /locus_tag="IWO38_001096"
     CDS             complement(12335..13021)
                     /locus_tag="IWO38_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ0313196.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            13495..13815
                     /locus_tag="IWO38_001097"
     CDS             13495..13815
                     /locus_tag="IWO38_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ0313197.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            13969..14874
                     /locus_tag="IWO38_001098"
     CDS             13969..14874
                     /locus_tag="IWO38_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0313198.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            14871..15644
                     /locus_tag="IWO38_001099"
     CDS             14871..15644
                     /locus_tag="IWO38_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0313199.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15825..17249)
                     /locus_tag="IWO38_001100"
     CDS             complement(15825..17249)
                     /locus_tag="IWO38_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0313200.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            17460..17906
                     /gene="nrdI"
                     /locus_tag="IWO38_001101"
     CDS             17460..17906
                     /gene="nrdI"
                     /locus_tag="IWO38_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ0313201.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(17971..19953)
                     /locus_tag="IWO38_001102"
     CDS             complement(17971..19953)
                     /locus_tag="IWO38_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0313202.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(19967..20863)
                     /gene="murQ"
                     /locus_tag="IWO38_001103"
     CDS             complement(19967..20863)
                     /gene="murQ"
                     /locus_tag="IWO38_001103"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ0313203.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(20866..21927)
                     /locus_tag="IWO38_001104"
     CDS             complement(20866..21927)
                     /locus_tag="IWO38_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0313204.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(22138..22665)
                     /locus_tag="IWO38_001105"
     CDS             complement(22138..22665)
                     /locus_tag="IWO38_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0313205.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(22732..24234)
                     /locus_tag="IWO38_001106"
     CDS             complement(22732..24234)
                     /locus_tag="IWO38_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ0313206.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(24492..25613)
                     /locus_tag="IWO38_001107"
     CDS             complement(24492..25613)
                     /locus_tag="IWO38_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0313207.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYSLGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            25739..26680
                     /locus_tag="IWO38_001108"
     CDS             25739..26680
                     /locus_tag="IWO38_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0313208.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(26830..27726)
                     /locus_tag="IWO38_001109"
     CDS             complement(26830..27726)
                     /locus_tag="IWO38_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ0313209.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            27995..29335
                     /locus_tag="IWO38_001110"
     CDS             27995..29335
                     /locus_tag="IWO38_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0313210.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            29553..29996
                     /locus_tag="IWO38_001111"
     CDS             29553..29996
                     /locus_tag="IWO38_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ0313211.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(30500..31918)
                     /gene="nhaC"
                     /locus_tag="IWO38_001112"
     CDS             complement(30500..31918)
                     /gene="nhaC"
                     /locus_tag="IWO38_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ0313212.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(32321..33544)
                     /locus_tag="IWO38_001113"
     CDS             complement(32321..33544)
                     /locus_tag="IWO38_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ0313213.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(33575..34414)
                     /locus_tag="IWO38_001114"
     CDS             complement(33575..34414)
                     /locus_tag="IWO38_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ0313214.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(34431..35111)
                     /locus_tag="IWO38_001115"
     CDS             complement(34431..35111)
                     /locus_tag="IWO38_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0313215.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(35113..36186)
                     /locus_tag="IWO38_001116"
     CDS             complement(35113..36186)
                     /locus_tag="IWO38_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0313216.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(36833..37504)
                     /locus_tag="IWO38_001117"
     CDS             complement(36833..37504)
                     /locus_tag="IWO38_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0313217.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(37591..40269)
                     /gene="mgtA"
                     /locus_tag="IWO38_001118"
     CDS             complement(37591..40269)
                     /gene="mgtA"
                     /locus_tag="IWO38_001118"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ0313218.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFTTEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            40928..41365
                     /gene="ndk"
                     /locus_tag="IWO38_001119"
     CDS             40928..41365
                     /gene="ndk"
                     /locus_tag="IWO38_001119"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ0313219.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(41387..42253)
                     /locus_tag="IWO38_001120"
     CDS             complement(41387..42253)
                     /locus_tag="IWO38_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0313220.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(42413..44506)
                     /locus_tag="IWO38_001121"
     CDS             complement(42413..44506)
                     /locus_tag="IWO38_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0313221.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT        13074 a         9412 c         7999 g        14648 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac ggagggtcag ctgattgaac actgtttagt ggagaggttg tgaaatcagc
      181 gtcctgacct gagtattaga gaaacaaatg gcaaaatagt tcctcatatt ttgctatttt
      241 ttgagctaat accgagggtc agctgattga acaccgttta gtggagaagt tgtgaaaaag
      301 tcgcctactt tcgagtcgta agaaattttt ttgaaaatgg ttcctcacaa ctttgcataa
      361 atttgactta ataccgagaa gtcatgatcc ccatctaact ccaaatcaca aggaatgagt
      421 tcaaaaacta gcttttctca acaaatatta cataaaaaat tctatgtttt ttgttaaatt
      481 tatttgcttt tttcaaaagt atgttacagt aatttcccaa ggaggtttta cagaaatgaa
      541 ttttgttgcc atggattttg aaactgccaa tcatcagcct tatagtgctt gttctcttgc
      601 gctagtcatg gtaaaaaata gccagatcgt tgatgaattc tatacattga tacagcctga
      661 gactcctttt ttctggcgaa atgtccaaat tcacggaatc catcaagaag acgtacgcaa
      721 tgcgccgaaa ttcccagaag tctggcaaac tatccaacca tattttcaaa aaaatcgttt
      781 agtcgttgca cataatgctg cttttgatac aaaggtctta gcgggatgct tagattatta
      841 tcatcttgaa cagcctaatt atctttcttt atgtacagtg aaaagtagcc gtcgcctgtt
      901 tcctgaattt ccaaatcatc gattgaacac tgtgtgcgaa aatttgaaca tccaattaga
      961 tcatcaccat gatgcattgg aagatagtcg tgcatgtgcg gaaatcctgc tttatcaaga
     1021 aaaaaaattc gggacagatc cattgaaaaa acttgtgact ataaagtaaa agaatactta
     1081 tgaaacacac aaagaggtcg tgagcctgcc gtttcactcc gagtcacaag gagcaatttc
     1141 aaaaaatagc ttctcatgtt tattaaatat tgtgactggt gaccgaggag ctggcaggag
     1201 aacaccgttt attggagagg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagcc
     1261 aaacaaaaat agcttctcat atttttcatt tggttggact tattaccgca gatgcaagct
     1321 tttggacacc gtttaccaag aggttgtgaa atcagcgttt tgacctgagt attagagaaa
     1381 caaacggcaa aatagttcct catattttgc cgttttttga gctaataccg aaggtcagct
     1441 gattgaacac cgtttattca aaaaaaatgg actgcgacac gacttttgtc acagtccctt
     1501 cgtgtgtttt tctgccttct caaaaggatg acagtttctc actcttttcc tatttcttct
     1561 attcaatcaa ataactcatt ttatatacat ctaaaaattc acctagatct gttcttgccc
     1621 ctctaggcat gaccgcttcg atttggaagc ccatttttcg gtaaaggtgt acggctcttt
     1681 cattacgaac ttgaacgtca agctccaatc gaaaaagtac acccatttac ttcgcccaac
     1741 tgatgatttc ttctagcata agagtgccta gtcccattcc ccaatattcc tgcagaatac
     1801 tgataccgac ttctcccacg tgagacaaac gaaattgact gtctgccttg acagatgctg
     1861 ttccaataat cgtcccttca tagattgcaa gcagcaataa attattattg ctttcgtata
     1921 tataatccaa ctcttcttct aaggtttctg gtggtaacag gagttctgct tcatccaaaa
     1981 ctaaaaattc tgtctcctta ttaacttgtc ccatcatcaa aagaatgtct tttgcatctc
     2041 ctggcactgc ttcccgcaag atgatcccaa cttcttcact catgattttc agcccatttt
     2101 tctagaattc gctgtttgaa ggtttcactt tgctccccgt atgagattag tttcacatac
     2161 cgcttttcta aatctgtatc acttttttct aaaatcagtt ccaaataatc ctccggcagt
     2221 gcttcttcta atagatttcc ccattcaaca acggaaagcc catcaccttc aaaatattca
     2281 tctaacccta aatctgctcc gctagctgct acccgataaa tatccatatg atacaacgga
     2341 atccgacctt gactatattc tctaataatc gtgtatgtcg gacttttgat catttgcgtt
     2401 atccctaatc cttgggcaat tcctttcgtc aacgttgttt tacccgcacc tagatcacca
     2461 gtcaatatca gattgtcacc tggcattgcg gaatgaccaa ttgcctctgc gaattgttct
     2521 gtcattgtta atcctgatag ttcgatcatt tttcatcttc tcccgctctt ctttcttttt
     2581 gccagtatat cgcgtaatga agttttttta aagaaaaaat gaactagtca agaacagcag
     2641 ctgctgtttt tggctagttc gcgtgtattt gttaatcaat caatgtttgt gctgctgtga
     2701 taatcgataa tttatataca tcttcttcat ttgctccacg agacaaatca gaaaccggtt
     2761 tgttcaagcc ttgtagaatt ggtccgattg cttcaaagtt accgaaacgc tgagcaattt
     2821 tatagccgat atttcctgac tgcaattctg gaaatacaaa gactgttgct tgaccagcta
     2881 cttctgaatc tggcgctttc agttgtgcaa ctgaaggaac ataagaagca tcaaattgca
     2941 attcgccgtc gatcatgtat tgtggcgcta gttttttagc gattttagtt gcttcaacta
     3001 ctttgtctac ttctggcgat ttcgctgagc cttttgtcga gaagctcatc attgctactt
     3061 ttggttcgat atcgaacaat tctgctgttt ttgcagaatc tacggcgatt tcagccaatt
     3121 cttgcgcagt tggattcacg ttgatcgcac aatccgaaaa gacatatttt tcttgatctc
     3181 ttccacgtac catgatcatc gctccgcttg tccgactgat gccaggcttt gttttgatga
     3241 tctgaagtgc tgggcgaaca gtgtcgccag tcgaatggat cgctccagag accattccgt
     3301 ctgcaatccc catatacgta agcatcgtac caaagtaatt cacatctttt aaaatctttt
     3361 ctgcttgttc tttggatgcc tttccatttc tacgttcaac aaaagtatcc accatttcat
     3421 cccacttatc gtaattatca gggctgatga tcgtaaagct agatgttttg attcctctgg
     3481 catgcgctgc atttcttatt tcttcttcgt tccctactaa aatgggttcc acaagttctt
     3541 ctgctttcaa ccgggcagct gcgcctaaaa tacgagcatc agtagcttcc gggaaaacga
     3601 ttttgatccc acggcgaaca attttaaatc ttaaactatc aaacaattcc acaagatgac
     3661 ctccagacgt ttttatatgt attatcatac actattccag aaaaaaagaa cagtttattt
     3721 tatctgtatc agtcaaaaat gctggaaaaa ttaagttttt cacatacaaa gcgaactgca
     3781 ctataacagg aaagcgctgt tttattacac tgtttcaggc agttgccaat caatcggttc
     3841 ttctcctaac gcaaggagcg catcattcgt tttcgagaag ggtttggaac caaaaaagcc
     3901 acgacttgca gataacggac tgggatgtgg agatgttatg atgatatggc gactcttatc
     3961 gatcattttg attttttctt gtgccggttt tccccacaag ataaacacga ttggtttttc
     4021 ccgttcattt aatttttcaa tgatcgtgtc tgttaatctt tcccaacctt tcccgcgatg
     4081 agaatacgct tgcccttccc gaacggtaag aacggtgttg agtaaaagga ctccctgttt
     4141 agcccatgac actagatttc catgttgaac aggtgcgatt ccaagatcat cataaagctc
     4201 tttatagata tttcttaacg atggcgggat ctttacgcca ggttgcacag aaaaagataa
     4261 gccatgagct tgattttctc catgataagg atcttgtcct aaaatcacca ctttaacttc
     4321 ttcataagga gtcaattcca atgcttcata gatatgatac atatctggat ggatcttttg
     4381 ggacgcatat tcttttttta aaaattctct tagatgcaga taataatctt ttgaaaattc
     4441 tgaggacaaa acttcttgcc aactgttatg aatgatttct ttcaaaaact ccacctccta
     4501 ataaaaagaa taactcacat tttcttcaat cacaagggac aatcttcacc ctcagcactt
     4561 gattaatatg gtaaactagg aacaagaagc agaaaggaac gataatcatt atgatcaaat
     4621 taattgcatc agatatggac ggcacattat tagatgccca tatgagtgtt tctcctgaaa
     4681 atgccgaggc gatacgtttt gcaaatgatg ccggtgttga gttcatggtc gctactggtc
     4741 gaaactatca agaagctcgc gcagctttag atgaggtagg aatcgattgt gcgatgatca
     4801 ctttgaacgg ggcacaggta tttgataaag aaggaaattc attgtttacc gtgtcgattc
     4861 caaataatca agcaatctct gttttagata ttctagatgc taacggtatt tactatgaag
     4921 tagcaaccaa cgatggtctt tattctgaaa atcaagcgaa acgaattgaa agttttgctt
     4981 ccatggtggc tacccattta cctcacctta cgtataaaat ggcgattgcc atggcctcag
     5041 ctaagctgga gttattgcac atcacatacg tggacagtat tcgtgctatt ttggaagaaa
     5101 agaatctgga agttttaaaa attatttgtt ttcatacaga agggccagct gtattaggtc
     5161 cagttggaaa gcagatttct cttttaggcg atctagccgt tacttcctct ggtcaaaaca
     5221 atatcgaagt gaaccataaa aacgctcaaa aaggaattgc tgttgcccat gtcgctcacg
     5281 agcgtgggat ttctttagaa gaagtcatga cgattggcga taacttcaat gatgtcagca
     5341 tgctgcaagt agcaggagtc agtttcgcaa tgggaaatgc agaaatcgaa gtaaaagatt
     5401 atgccaaata tacaacggac acaaaccttg aatctggcgt aggaaaagcc atattgcgcg
     5461 cgattgcaga agacttgtag ttttatctag aaaagaggga tatagatgtc agaatttttc
     5521 attcaggaaa agcagcttgg acgtgcaact cggacaattg tcaaaaacga aaaaggacat
     5581 tccctgtttt tgatggttgg tcgctgggga acaaagggcg atgcattgtc tctttatgcc
     5641 atgaatggcg atctcgttgc tcacatcaag cagataagcc tgacattcgg tacgcgtttt
     5701 gagctgtata aagagttcga aaaggtggga accatgcaga aaatattcaa ttggcccggt
     5761 gacttttatt atattcgtag acttcactgg acagtccaag gtgatatcta taatcatcaa
     5821 tatcaaatcc atcatttcaa tcagcaaatc atgaaaatgg ataaagcaac acttttttca
     5881 ggagattact atgtattgga tgtctcaaat gatgaagatg ctccagtttg tatttgtatt
     5941 gctgcggttt tagattactg gctctaccgt aaagacaaaa gtcgcgataa gcctgtttct
     6001 taccgactta acttaagtta aaaagatgta cctgaaatgc ggaaatacgc atttcaggta
     6061 catcttttta gatattcttt tgcatatgat cataaagatg accactgatc atccgctgac
     6121 cgacaacttt gaagtcatgg cgttcgtata attttttagc ctttggattt ttttcatcca
     6181 cacttagacc gatgacggag cgattagctt tcttcgccaa tttaggtaat gcttctaata
     6241 atcgcgaacc gatgccttgt cctctaaaat cttctgatac actgatagag tccaaatacc
     6301 attcgttggg aaacgtttct ttatcaatga acaaacgtgt agacgcatct agctgatatt
     6361 tctctaagat aggtaccagt ggctgatcga tgattgattc ttcttcatct gtataaccgt
     6421 aagcaactcc tgcaacttta ccgtctattt cttctacgat acctcgtgaa taactgtaac
     6481 gataatttgg atcagtgatt gcttcttcta aaatctctaa agtcttttgt tcccctgtct
     6541 gcttcaaaaa tggcagttcc atatctttta gaatcactaa gatcaatggg gcgatttctg
     6601 gagcatcttc ttttctcgca aaacgaatca tagtttcctc tccttttctc cattctaaca
     6661 tgcagaaaaa gaaatcgcaa aatcgttatt ttaaaaaatc ttcaatcttt tcagtaatga
     6721 aataaccatg agtaacgatt cttgccgctt cagttcggca ataagggcag acgactggca
     6781 tttctttatc attatgttcg actacattat tgacttcttc gtatgcaaat ttttttccgc
     6841 aattagggca tttcattttt tccatgttat ctcaacttcc tttcatcttt actctcttag
     6901 cttactctta tattgtaaaa aagagaagca atatccttct tctatgcaga tatccatacc
     6961 tgacggtcct gttctccatc tattaatcgg actttataat aaggacgttc atctttgact
     7021 agaagtgtcc actctttcac ttttgctttt ttcccgtagt agctgactgc ttccttcatc
     7081 gctgcttgcg gttgtttgaa ctcttccgtt ttttctatat caaaaagatg ttctgcggcc
     7141 ttttccgtgt catttcgtac ggttgttttc ccagtggatg gattgactgc tatgacttgt
     7201 ccttcacaaa aaaactggta ttcatatgtt gcattcgtag gattctccaa atctttttca
     7261 actgcaaaat gaatagcaga tatgttatgt tcttttgttt cgttttttac gattgctgct
     7321 gcttttttca atgagatccg atattcctgg tcactaatat catccatgtg aaaagcggca
     7381 ttgagcagtc ccgtcatacc ggtaatccct attacgatga gggcagcaac accaaacatc
     7441 atttttttca ttcttcattc tcctgtttct tttttttaaa gaatatcaag aaatgaacga
     7501 atgaaaaatc gaatgaaacg aaaaaaaagt gacaattttg ttatctttat tcgtgcagtt
     7561 ctaatggcaa tccgtctgga tcaaagaaaa acgtcatttt ttttcctgta aaggtatctt
     7621 ctctaatcgg ttctgattcg atccctaagg actgcaattc tgcaattgtt tcttcaatat
     7681 cgtctacttt gaaagcaaga tgacgcaagc cacaagcttc tggatagttt ggtcgttgtg
     7741 gcgcattagc aattccaaaa atctccaatt cagcacttcc tagtttcaga tctaatttat
     7801 agtcatctcg ttctttgcgg taattttctc tgattacttc aaatcctaat ttattaacat
     7861 aaaaatcacg tgatttctga taatctgaaa caatgatggc tatatgatgg atcgttgtaa
     7921 ggttcatatg agcactccta ttcttcgttt aagctcggat ctatattaat ctccagttcc
     7981 attgtaaatg tgtcagattg aaaaaaatag ctgtttttcc tttttataag aaaaaaggca
     8041 aaaaaaacag aaagtaggtc ctccaagaca ctcttcctgt tcccaatata ataaatgaaa
     8101 gtaagaaatt atattttcaa gaaaacaaac agaaaattat cataacacaa attatttata
     8161 attgaaagac tagttttttt tcaagaaaag catacaatag attgtgttag taaatacaga
     8221 acacactaca tttagtgtct taaggagtcc tcatacatga agtcatattt tcagttttta
     8281 gctcatcagc taaaaggctg gccgcaacaa aattattatc ttttcttttt cagtcttggc
     8341 tgccaggtca tgactcttgt caatagtccc atcacttggc tgtccgttat tacatttatc
     8401 ggtaccacgc ttggtgtatt gtgtatttta tccatcaatg cggcaaaatc agtcaacggt
     8461 attttaggta ttttgtctgc gatttgtttt attatcgttg ggttttcagc aaagaactat
     8521 ttaagtatcg gtgaacagat tgcttatatg atcacattgg atatccccgt attgttaagt
     8581 aaagaatgga atcataatat ggcttcaaaa atccgaaaat tcactggaaa aagctggttt
     8641 atcgcaatca tcagtacttt ccttgtctat cttgtttcag gattcctgat tggtcgttat
     8701 acagatgatc ctcggccgtg ggtagatgcc attgcttttt ctattagctt gactgctggt
     8761 atcatcagtt tcttgcgtta caacaaccaa tattactggt ggttagcttc agggcttgct
     8821 caaatggtcc tttggtatat ttcatttacg catggatctg caagtttggc gatgttcgtg
     8881 aacagctctg tttacttgct gaatgatgtc cttgcattta ccgtttcacc ttggtataac
     8941 caaaaagaac gtgaacgtat gcaaaaaatg gaagaagaat actacaacgc acaacaacac
     9001 gcataaaaaa actgagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca
     9061 cgtataaacg gcggaatcag aagcaactcc ttcggaaata agctgaaatt cacaaaaatt
     9121 tgaaaagcaa ttttcgtgaa tttcttctta tttcttggag ttaaacgctt ctgtcccagc
     9181 ctctattttt tatagtaatt ccttcagata ctttcttgcc tcatcattga aacgcagtga
     9241 ttcttctaac ttctgataat attccaacgc ttgtttctga gcttcagtaa aacgataatg
     9301 ttttcctttc catttgtcga ttgtctcttg cacatttcca gattggatat ctttcactac
     9361 tagttgccat ttttcttttg agactgtacg gtctttctta tcagcagatt gtatagcttc
     9421 tcgttcttta cgcaatactt ttaaaaaacc atttctgact atccgaggaa tgctgtcgaa
     9481 gaacccttct gtaaaaccat agcgacaata ttgcttccaa ataaatagat agatttttgg
     9541 ttggatgaat cgttggtttt tcgcaaaata ttccatctga gcagtgactt ctggtgttcg
     9601 ttgacgtttt gtctttctca acacggaaat aaatgtttca aaataagcat taggatgtac
     9661 tggcacttct tcatatacga tggattgcca atctttacgt aaaaatgcgc gatagttgat
     9721 caagaattct tctacatcct ttactaattc ttctgcaaag tcgtcaaata gttctttttt
     9781 tatctgtcgc ttttttgact gcccattgat gtgttcactt attttcttga tttcattttc
     9841 caatgcttga taatccgcta tgtaaatcgg catttctact tggcgaaatt ctgcgagccg
     9901 ctgagctaat tttatatccg gtaaatatgg ctgtttcatc cgacgatttt ggtacaagac
     9961 aaaacagtat ttttgccata gtccttcagg aaaatcgata ttcttctgct taagccataa
    10021 taattcgtct aaggccagat caacttttgt cattccctga tggatcgaag ctgccactgt
    10081 cggagagaca tgcaggtgat cggcaaagtg gttgatgcct agttttagga tctttccggt
    10141 ttttggcgat ttaacgatgt attgcacttt taattctcta ccacactcac aatacaattt
    10201 ttgctggctt cgttttgttc gaaaaaacgg attgatcttg aagtcagaaa acacccaatc
    10261 tgttgcagct aaataatgtt cttccataaa acgagagctg attagatatc ggcggtgctt
    10321 atcgactagt tcccgctgtt ttttcgttaa ttttgcatag atctcccaac gttgactgct
    10381 tgtgtattgt ttagaaattt tgatgggata gcttttgctg atttcttttg gaaaccattc
    10441 ttcaaagacg gattgccatt cttccacggc tgtttctttc ctttctaatt acaaaaagct
    10501 ggctactaac ataacgttag caaccagcca gtgtttcttg ctaaattagt gagttgttcc
    10561 ggcatttccg gaaatatctt ctttattttc tactgctgct tcaactgcat aagtcaaaga
    10621 agaagcaatg tcttctaaag acatagaagg cgtacccact ggacgatcga tcacttgatc
    10681 gggtgagaat ggcacgtgga tgaacccacc tcgaattgcc gggaattgtg tttcgatcaa
    10741 atgaagcaaa cgatacatga tatcattgca gacaaatgtt cctgctgtat aagagatgta
    10801 cgctggtaga ccatgatctt gtacattttt tgtcattgct ttgattggca atgaggtaaa
    10861 gtaagcggct ggaccgtttt cttctaaagc cgtatcgaat ggctgatttc cttcattatc
    10921 agggattctt gcttctgcta gattgattgc gacacgttcg aacgagactg ctgaccgccc
    10981 acctgcttgt ccgacacaga tcacgacatc tggttggtgc tgttggatgg cttctttgac
    11041 tacttgactg cttttttcga atacggtcgg tacttccact ttgatgattt cagcaccgct
    11101 gatttcatct ggcatttttt ttactgcttc ataggctgga ttcactttgt ccccaccaaa
    11161 tggatcaaaa cctgttacta gtactttcat ctgttgttcc tccgtccgat tgatttaaca
    11221 tatggttatc ttttatcata ccattttcct tgaacagcgt aaataaagga aacaatagtt
    11281 taaataacat aacagatcaa acttaatata ttcttataaa aaaatagtgt tccactacaa
    11341 tgttagaaga ggttgcgaca attattctgt cacaacccct ctcttctatt atttaaatag
    11401 aatcatataa gcaatttgga aaactagcat aaataacgca ataaacaact ggtttttgat
    11461 cactccgtat ttgtctttca tctccagcat cgctacaggc acgatattga agttagcagc
    11521 catcggtgtc agcagggtcc cacaaaaacc gcaagtcaat gcaaccattc ctattaacgc
    11581 tggattcgct ccatgactaa atacgaatgg tgcaccgatc ccaaccgtca tcacggtgat
    11641 ggccgcaaat gcattcccca tgattaccgt aaatatcacc atgccaagag cgtaaatgat
    11701 gatgccgatg ttcacatttc cttctggaac gatcgtacct actgcattgg aaatcactgt
    11761 cccaactcca gcgcttgtaa ataccgcacc tagagaagct aaaagcattg gcaacatgct
    11821 taacggccct actgtgccca gcatatctgc tgcatcatct aaaaatgtac ttggtcgatt
    11881 atctcgagaa aagaacatca agattagcat ggctgcaaaa acaccgacgc ccacgccgac
    11941 taaagcacct aaattggtaa atagagcaaa caagatcgca aagacaccga tactcaaagc
    12001 tggaataaaa attttcattc ctagtttgtc tgacattttt tccatatatt cttttgatgg
    12061 aagccggctt tctcctttgc tgactttctt agcaattgct ggtatggtca tcgcaaagat
    12121 caagccaccg ttcacgattg ccggaagcca tcttcctcct gcaataacta cccctaagac
    12181 cgtccagaaa aaggctgacc cataacgatg aggattggtt ttatctgtta aatttcttac
    12241 tccagtgtaa atcaatacga gtcccatcac gatataaagg atctccagta atttatcgcc
    12301 taataaaata tcaggatttg taaaaaagtc catcttattg cccctttcct ggataatatt
    12361 tcttcgctaa tttcttttct ttaagataga agtagacact tgccacaact aaagccgtga
    12421 tagccactgg aacttccact aaagcaagct gtcccaatgt tacgtcatat cctagatctt
    12481 tcagtgttga ttggactagc aacgcccctg ctcctccgat gaatagtact tggccaaaga
    12541 accagcagac attttccata gctgaagcca taccttttag ttcttcttca tgcttaggat
    12601 ggacttctaa cccttttgat tcgatggtcc ccaatgccat cggtaaaatg attggacgaa
    12661 caaatccagc aactccacca aaactcacgt taaaagccgc aaagatcatt cgaaagatcc
    12721 cataaatatc tacaatcata ccagcagata ctttcttgaa tcgcttgatc aaggaagccg
    12781 ctgattcttt cagaccattt ctttcaagcg ttcctgttgc caacataatg atgataaaaa
    12841 tcgccatccc gcgattgttt acaaaacttg tacctaaaag ttctaacatt ccttgaatgc
    12901 caagaccgcc agtcaaagct gtaacaacta aagcaaccat tacgatcaat atcgagtcta
    12961 atttaaatgc aaacccgaga ataacaatca agacgcctaa aaggctaaga taattttcca
    13021 tatattttcc tccataaaat gacaatattt ttttaattat aaagcaaaac acataaaaaa
    13081 tatagttgtt ttctcacgaa atagacacta tttttaaata tttttcgtta tttttttatc
    13141 acaaaaatta tcttacttat tgtaaggtaa agcttttttt aatttattgc ccgataatat
    13201 atagaaataa ggtctagtta ctgttttttt taaatgatta ataaataaca acttctttct
    13261 atgaaattgc aaagcattca tcttcttttc cgctaacgat atatccgtca gtgcttggta
    13321 cactttacta aaaagtatga gattgccgat atctggatag gtgtgctata ctacagaaaa
    13381 agatatacaa tctgccaccg ggtaggataa agaggatgat cattccatta ggtctttgaa
    13441 gggatgattt tcctcttttt gtgtattttt ttatagttaa ggaggaatgt aagtatggca
    13501 tggatggaat taatcatagc aggaattttg gaagtattct ggtcaacgat gatgaaatgg
    13561 agtgatggtt tttctaaaat caattattct ttttacacag tgattggcat gattgctagt
    13621 ttttactttt tgtcaaaggc aatcaaaagt ttgccgatga gtctctctta tcctatctgg
    13681 acagggattg gcgcagttgg ttcggtcata attggtgtgg tcttgtttca tgataagctg
    13741 aatctttcca cttggttctt tgtggggatg ctactcatca gtatcattgg catcaagatt
    13801 actagcggcc actagacact tggaataagt actgatcaga aaaaataagc ctttttattt
    13861 aatattcaat ttttttatga aaaaataaga aataagtcca atgacctagc gacttctcca
    13921 ctcatcaggt gtatgcttgt actatctata gaaaaggagt gaatattttt gaaaagtctt
    13981 tcggtccaag aattatccaa gaactttggt cacaaacaag ccctcagcga tgtcagtttt
    14041 agcttatccc ctggcaaaat cgttggatta gtaggtccta atggcgctgg aaaaacaacg
    14101 atcatgaaag ctattttagg actgatccat tactcagatg gaacaatctg tattggtgaa
    14161 aatgagattt ctccctctag tcataaaggg ttggaacaag taggagcatt gatcgaatac
    14221 ccgggtatct atccattctt aacaggctat gatcatctga agttattctc tgaaaccaac
    14281 gatgtatctg ctatcgacac tatcgtgaat cagctcaaga tggaaaagta cattcacaaa
    14341 aaagcgaaat cctactcgtt gggaatgaaa caaaaattgg gaattgctct tgccttactg
    14401 aaccatccgt ctatcgttat tttagatgag ccgatgaacg gtttggatcc acaagcaaca
    14461 agagatgtgc gaaaaatcat taccgacttg gctactcagg gcacctcttt cttgatctcc
    14521 agtcacttac tcagcgagct tgaaaaaatg gtcgatgagc tgatcctgat caatcaagga
    14581 aagatcatca aacaatgtac aatggctgaa ttgaaccaaa cagcgcaaga tttcatcgtt
    14641 atctctacaa caaatgatct agctgctcga acgatcttga cagaagctgg atatactttg
    14701 gaagatcctg ataaaatcaa gcttttaaaa gaaaacgaag aattattagg aaccgtgatc
    14761 cagctattgt ctgcacaaaa tatccatgtg acagacgtcc agcatctgac gaatgatttg
    14821 gaatcttctc tattggaaat tctggattca gtaaaggggg aagaagaaaa atgacattgc
    14881 ttgtaaaaca agaattattt aaattgatca agaaaaagtc aactgctgtt ttatctgttc
    14941 tattagttgt tttgctcata ggaacagcac ttctagccaa aaaatacacg acgatcattg
    15001 atccagttga aatgactgct cagcttttta gtgccacatc ttggattgtc tttatcatga
    15061 ttgcagctgc aagcacgatc atttcgatgg aagcacaata tggtacattg aaaaatctcc
    15121 tttatcgaaa gtattctcga ggagaaatat tagtaagcaa atggatcaca ttagttattt
    15181 attctgttta tttatacctt ttagctatta ttgtcactgt gttgatgaaa ctgattcttt
    15241 tcccttcgat ttccttcacg gagaaggttt ctactggtca aacactgatc caaagcttgt
    15301 tcacttatac tttgggtagt tatatcggtt tatggctgat tttaagttta gtgttgatga
    15361 ttgcttgctt tatcaatagt tccggtgcat ccatctcagc aggaatcgtc ttttattttg
    15421 cttcgtcaat catatctggt atccttattg cactcatcca aaaatgggag tggatcaaat
    15481 ggaatccgat cagtatgttg aatctgcaaa accaaattgg aaacgaagag atcatgaagc
    15541 aattgactca tctttctact aatcaaatgc tattcggtaa tttagcttac atcgttttgt
    15601 tcttagcatt aggatatctc gtatttaagc gaaaaaatgt atgatgactc atcaaaaaag
    15661 aggctgtgaa agagacgttc agccttattg ccgattggct gtctcttaaa caccgtttat
    15721 tctgaaaaag gagctgtgac aacatttgtg tcacagctcc tttcctagta tgtttttgaa
    15781 taaacgacag gctcacaacc tctctttata cttcttttat acttctatac gtgaagcatc
    15841 ccagtggcaa ataaaaccat tgcaatcact ccgaccatcg tcacacctat aagtaaaatc
    15901 ttatctaagt ggatatgttg tacaggctcg ttttgctcct tacgtgcttt gaggtaaaaa
    15961 taaatgcctg gcaagaaaag aacaagaaca agcattactt cattaaaggc tacgactagc
    16021 atagcccata cttgaaaggc acttgctaaa agccctacta aaaattggac ccattctttc
    16081 cgctgataag aaagtttcat ttggtacaat cctaagaaaa gccacgtgaa gaaaatagct
    16141 gctgtaccaa gtgtgtaagc aaagttataa gcactgtcag tgaataataa tgaaagtaaa
    16201 aagacattcg tacataacgt cgtcaatacg atagcataag ttggtgcccc atttttgttg
    16261 agttttcccc atgctttagg caaaagtccg tcttcagcca tgtttcccat tgtttcaatc
    16321 ggtaagatcg tccatgagag ccaaacacca actaaagaaa tgatcaaacc gccgttgatc
    16381 acgactgctc cccatttacc aaccatcatt tcaaacacat agcccatagc aggctgcgcc
    16441 ttgatcgttt gcagctgatt catcgtcatt gtcccatatg gaagcatgga aaccaagacg
    16501 taaataataa tcaagccgaa tatccctaga atcgttgctt tcccaacatc tgattgtttt
    16561 tttgctcgtg aacctacgac agaggctcct tcaataccaa caaataccca aagcatcacc
    16621 atgatgcaac tcaatacttg actggaaaac gggatcggct gtccattcgc attcaatata
    16681 atgtcgctag aaaagttatc aatgaattgt tggatcgaaa acgtgctgat agctactaca
    16741 ataaataaaa ataatggcac taatttacag aatgtgacga tagcattgac gaaactagca
    16801 ttttcgactc cccgattcac tagccatgca tatccccata aaaagactgt actcatcacg
    16861 atcgatgcca gattttgccc accttcaaat actgggaaga aatatcctaa tgcgctcatc
    16921 aacaatgttg caaaagctac gttgcctaac cagcatgtga gccagtaacc ccaaccgctg
    16981 ataaaaccag cgaaacggcc aaaaccttct tctgcgtaac taaagatccc gccttttaaa
    17041 tctggacgtt ttttattgat attattgatc gacaaggaga gcatcaacat tgctgttcct
    17101 acgagtaccc aactgaaaat agctgggccg ccagccgatc cggagatgtc cgtcataata
    17161 ccaaatacgc cacctccaat cgctgaacca attactaagg cagcaagtgg aattaaacca
    17221 attccttttt tctgttgctc tgtatccatg taagcatcct ccaaaataaa accgctttta
    17281 tcaaaaagta taacaaacct tttttatttt tacttaatta ataaaaactt taatagaaat
    17341 ataattctac cggctttttt ctcattatac aagcaaagcg cctagtccct ttccaaatta
    17401 caccaccaaa aaacttaaaa aagatgctat actaaaagga aagaattgga ggatgatcca
    17461 tgaacatttt atatatatcg atttcaggaa atacgcgtgc ctttgccaaa catctagcag
    17521 aatatgctga aaaaatgcat gcagaagatc ctgtcaatcc tgaagttact ttaaaagaaa
    17581 tccatgaaaa tagtgatttt acgaaagaaa cagaaccatt ttttactttc gttcctactt
    17641 atttagacgg cggaaatggt ttagataacg gtgatacaga aatcttgacc gaaacgatgc
    17701 gtgaatatct ggaacatgaa gacaatcata aactttgtct tggtgtagtc ggtagcggaa
    17761 ataaaaactt caataatcag tattgtctaa cagccaaaca atatgcacaa acatttggct
    17821 ttcctttctt ggcagattat gaattacggg gaacaccatc tgatgtgaca cgaatttatc
    17881 agattttagc agaaaacaat aaataataaa aaagaagaag ctgaaaaata gatttcagtt
    17941 tcttctttcg tttctattgt ttctatcata ttaagctttg acatccatca caggtgcttt
    18001 tgcttttgct ttacctgttt tttccaagac aaagtttttt acacgatcca tattggttac
    18061 caagacgatc atggatgttt ctttgcctgc tgctttgatc gcttctaagt cagcttctgc
    18121 tacaagcgta tcaggtgtca cttgctgccc ttctgttact ttcacatcaa atggcgtacc
    18181 gcctaagtca actgtgttga ttcccatatg aagcaagatt tctaaaccat ttggcgttgt
    18241 gatcccaata gcgtgtttcg ttgggaagac agagctgact accccagtaa ctggagaata
    18301 gatatgtccg ttttctggtt ctacaaagta gccttcaccc atcatctttt gtgcaaaaac
    18361 tggatcactt gctttatcta aagaaacaac tgttccgtca gcaactgatg agaggatagt
    18421 cacactttca gctgaagttt gaggtactac tttttcaggc acttcattta ctgtggctgt
    18481 ttcagtcgct aattcttctt tttctaaacg gtctttagga ataccgaaga agaatgtagc
    18541 aacgaagcca ccagcataag ctgccaataa tccaaggatg tagcctaacc atttaccgtc
    18601 agagatcaat gggatcaaag cggctcctga aggtccaata gcgatagccc cgacatttcc
    18661 gatcattccg atcactgccc cgccgatacc tccaccgata caagcagtaa taaatgggcg
    18721 tcctagaggt aatgttactc cgtaaatcaa tggttcgccg attcctagga tcccgactgg
    18781 taaagctcct ttgatcattt ctgttaattc tttatctttt ttgcagcgaa tccataatgc
    18841 caatgctgca cccacttgtc ctgcaccagc catcgcaaga attggaagca atagcgtacg
    18901 accagtttca tcaatcattt gcacatggat tggagtcaaa atttgatgca gtccgaacat
    18961 aaccatcggc aagaataatg tgcctagaac gaagccggca aatgctccac caacgccaag
    19021 tacccagttg atcccaccga ccatgccatc agaaatgaaa cctgctaaag gcatgatgaa
    19081 gaaaatttca atcagaccga tggcaatcaa tgaaagagtt ggtgtaacga tgatatctac
    19141 cgcatcaggt actactttat gcagtttttt ctcaacgatt gacaatagcc aaacagcgaa
    19201 gatgacacct aggatcccac cttgtccagc tgcaagagct tcaccagtaa ataagttttt
    19261 cagtggtgct tccggattca tccctgtcag catcacaacg gccccgataa ctccaccaag
    19321 agttggtgtt gcaccaaaaa cttgtgcagc attgatacct gtatagataa ctaggtagct
    19381 aaacatcccg tttttcaaga tgttcatcac atcgatatat tgttgccatg ttgctgcatc
    19441 aagtgttccg gctgtgacta agtttgctaa aactgcggca atccctgcca cgatcccagt
    19501 accgacaaat gcaggaatca ttggcacaaa gatattagaa atgtctttta ataccgattt
    19561 gattttagat ggtttttgtt tcgatttttg tgccgctttc atttcttgtg cccgttcgtt
    19621 tacacggtct tttccagatg ctgatgaatt tgctgtagct gtacttacag aagaaagttt
    19681 ttctcctaat ttcacgcctg ccaaatcaac catttgctgt gctactttgt tcactttccc
    19741 aggtccgatg atgacttgaa gctgttcatc ttctactaca ccaagcactc ctggaatcgc
    19801 ttttaattca gagacattta caagatcatc attgatgatc ttcatacgca cacgagtcat
    19861 gcagtggaca atgctttcta cattgcccat gccgcctgcc cctttataaa tttgttttgc
    19921 cagttcttcc aaagtcagtt cgttttttgc catggtcttt tccccctcat aaggtccctt
    19981 ttacaaatcc gtttgcttca gctaattttg cttgagcttc ctctttatca ctatttgtca
    20041 aaatcatgac gatagccagc ttaacatttt gatctgcttc ttcaaagtac ttttcagctg
    20101 tttcgtaatc agcttctgtc gcttgcatga tgattcgttt tgagcgttcg attagttttt
    20161 catttgttgg tttgacatcg accatcaagt tattgaatac tttacctata ccaatcatac
    20221 tgatagtaga aatcatattc aagataagtt tttgtgctgt tcctgacttc aatcgagtag
    20281 agcctgtcaa aaattctgga cctgcatcga cttcgattgg gaaatccgcg tgtttactga
    20341 tttcggcttg tttattgcaa gaaatcgttc ctgtgacagc accgatcgat tttgcgtaat
    20401 ctaatccacc gatcacatat ggtgtacgac cgctagctgc gataccaata actatatcat
    20461 tttttgtcaa attcagcttg attagatctt cttgccctag ctcataagaa tcttcggccc
    20521 cttcgacagc tacagtcatt gcttcttctc caccagcaat tagacctatg accatttccg
    20581 gttctacacc gaatgtcggt acgcattcag cagcatctaa cacacctagt cttccacttg
    20641 ttccggctcc catataaatc agacgtccac cttttttaaa ggactcaatc gtgttagcaa
    20701 tcactggttc gatacttgtc agttcttttt caaccgcagc ggccactttc tgatcttctt
    20761 tgttcattag ttgaacggct tgagaaatac tcatctcatc taatccaaaa gtttcttggt
    20821 ttctgcgttc cgttgttagg ttctctaaat tgattttgct catttttatc ttccttcttt
    20881 tcttataaat tgatagttca taccagcttt gatttggttg atcaatggta gctcttcttt
    20941 gataacttta gctactacat tcactttttc atccgctggc aaatcatatt tggttaattg
    21001 gatctcaccc atatagcgca aatacttttc gttatctaaa gtaatagacc ctttcatacg
    21061 cgtagctgta tttcttgctg ggataggcgg aatctctcga aaacgtgcat ctacgcttcg
    21121 tatgacatct cttgcatcgt cttgccgatt cgtatgcttc cctaatatat attcgtaaaa
    21181 ttcttcatct acaggttccg tatggagcaa gactgtctgt tctttttgga aagaagcaaa
    21241 ttgctcctga acgttttctg acagacctgc atctccgatg taaacaatat ccgtgttact
    21301 tgctaataga tctagagcgc ctgcaagagg atggatccct cgatgttctt ccaaagttgg
    21361 caatccttga tatagtgggc ctctcaactc tgtatctcct ggtacaaaac cttggacaag
    21421 gagaccttga attttgagcc attgattttt tttctgatac cattctttgt ccagccctgt
    21481 ttcgggacgt ggataataat tatgccaagc ttcaatctgt gagaaatcag cttctgcttc
    21541 tttcaattca tcgatatctt taggcgtgat cgtgctagca ttcaaactga ttttcaattg
    21601 atgtgaccag tcggcaatct gctgattgga aatatgataa tccattctta aacctgttac
    21661 accaattgct ttcaaaggcg ttagatcttc taatgaaaaa cccgctcgtt ccaatgcctc
    21721 tcctgaaata tcgaccatca attctagttt attctttttc gcttcgcttc ctagttcgat
    21781 caatcgcttt tgatatgccg aaacatcatc ttctggtata tgcatggaag tgaaaatacc
    21841 gacaaagcca ttggatgcca tttggcggat atactgtttc ttctcttctg acaaagaatc
    21901 gttcataaat attgaaaagc caaacatatt atccctcttt ttctgtatcc gtcttcattt
    21961 ctatgactag agtatatatc taaagaaaca aaatttcaat acttttatta aaataattaa
    22021 atttcatttc taaaggttga gtttatatta tcaaaagaat tgtcataacc taaaaaaggc
    22081 actgctaagc aaaagcagtg ccttgaaata ttttatttaa ataacacaaa aacataatta
    22141 ctcataagaa accaattctg tattatctac catgaatgta aagaacccct cattaggcat
    22201 cggatgaaaa taagcattga tcactcttat caaggcatta tggcaaacta acagatagac
    22261 attttcttcg tcttcgatgc attctttcaa taaaggaacg atccgtgcat acacatctaa
    22321 gactgactcc ccatttggga atcgtacagc gaacgctaaa cgtgcttttt gaaagttctc
    22381 atctttgctt ggcagtccat cataatcccc aaaatccatt tctaccaatc gttcatcaac
    22441 ggtcagcggc aagctgagac gatcagcaac tgcttgtgct gtatctcttg ctcgttgaag
    22501 aggggaatgg atgattttcg tgattggaac aggcaaatct actacctttt ctgctagttt
    22561 ctgtgcctgc atctgtcctt tttcagtcag cggcacatct gcccgtccgc aaactaatcc
    22621 ttgtgcgttc caagttgttt ctccgtgtct cgttacataa agcatgtgat tctctccttc
    22681 gttggcaaag ttccccttaa ccataccttg ttgttcttga gattacaagg gttagattct
    22741 ctcaagagaa ataatctcag tagatacttt atccaggaag gtttgatcat gttccactag
    22801 taacatggca ggtttcacgt tcaagatcag ttgttctaat tgttcttgat tgaagacatc
    22861 caaataattc aatggttcat cccatgtata tagttcagct ggctgtgata aagatttagc
    22921 caattccact tttttccgtt ggcccatact catctgctcg atcttgttat gaaaaacatc
    22981 tctttccatc ccaagctttc ggaggttgtt caaaaatgct tggtagtcta cttggttttt
    23041 ctctgcaaat tccgccaacg ttcctcgatt gtcttcataa ttttggcttg cataactaat
    23101 gctcagatgt tttggctggt atttttctcc tataaccttg ccgttgaatg cccccagaag
    23161 ataatggata atggatgact ttcctgcacc gttaggacct gaaatcgcca cccgctgatg
    23221 aggctcgatt gtaaaatgaa ttggctcgaa taacagattt tcatacccta attgaagatc
    23281 ttctacgctt aaaagtcgct tatggtgaga cgcttggcta ttcatcgtca aagaatcgat
    23341 atattcgata tcttttaata gtttttcttt ctcgctgatc tgggtctcca tccgattcac
    23401 gattgctttg gatcgtttca tcgtccgtgc agcatcagca cccactgctc ctttattcac
    23461 tcgtctagat tcagtatcga tgaatccgac ttgtttcttt gttttatctc cttctcggga
    23521 acgagaccat tcggcttttt cagctgctgt tttctttagc ctactgactt cttttttcaa
    23581 tttttcattt tgagccattt caaattcatc acgaagtttt ttctgttctt catagataga
    23641 gaaattccct tgataaagtt ccagttgact tttttcgatt gctaaaacat ggtccactac
    23701 ttcatcgata aatccccggt catggctgac cacgatgaag ccttgttttt tctttttcaa
    23761 ataagccgct acttgttttc taccagagat atccaaatga ttcgttggtt catcgattaa
    23821 cgggaaatga gtgtcatcca caaataaaag tgccagtagg acttttgtct tctctccccc
    23881 cgatagtgtg ctgaattctc tccataagat ttcaggatct gtttgcatca attggagctc
    23941 tctttcgatt tcccatatct caaaatccgt aatatcattt aacacgtaat aggttaaacg
    24001 ttctttatct tttgtctgtt gcgggaaata ggcaaattct tgctgatgga ttacttgccc
    24061 ttgataaggt agtttgtttt gtagaatatt cagtaaagtt gtctttcctc gaccgtttcg
    24121 tccgataagt cctagtttcc attgtgtgtc aaaatttaga tttgcttgtt caaataataa
    24181 tgtgccttgg ctgtcgtagc cgaatgtcag attttttatt tcgattttag acattttcca
    24241 tcacctcgta aaaaaagacc agtcctattt tcagaactgg ctaaagagga tgcaagggtt
    24301 ggatacacac aaaaagaccc atacatcgaa aaaaccaagc caatcccgaa aatctgcaca
    24361 ctgcttcctt gtctccacaa ggtacagtct gaatgcagct ttcatcaatc gacttaattt
    24421 ttcaatgcac ggatccctca ctttgtccta tatttgaaat tacgatagca taaagaaaat
    24481 gattttgtca actacttcgg gaggatggtg aaaaatctgc taagtgtctg tcctaagatt
    24541 tttgtttttt ctaatgtttg cctgtgttcc attgcttctt ctaaacctat tggacccaaa
    24601 tgaatactta agacaattgg gagaaaatca gaaagtcctt ctagctcttg gtctctactt
    24661 ccgcaaatag cgattattgg ttttcgatat ttttttgcaa gcactgctac accgtatgga
    24721 acttttccgt ggatcgtttg ctgatcgatc cgcccttctc ctgtaatgat gacatctgct
    24781 tgttcgatct ttttttccaa tccagtgatt tggctgacta aatcgaaacc agaaaccatc
    24841 ttcgctccca acaaaaaaag ggctccacca ataccgcctg ctgctccact tcctggaata
    24901 tcatctaaat ccacctccca gaaatctttt atttgctgag caatctttcc tgcttgttta
    24961 tctaagaaag ctagcgtcgt atctattcct cctttttgtc ttccaaattg ccaagcagct
    25021 ccttgttgac ctgtatatgg attggtaaca tctgcagcga taatcaattt tactttagaa
    25081 acaagaacgt ttcttcgatc aaaatgaagt tttttagtag ataataaagg atttccttct
    25141 gtgtaccctt ctaaagaagc acccaaggat tggagtaatc ctagaccacc atcgctacac
    25201 ccgcttcctc ctaaagtgac aataatttgt tctacttctt gaagcagtgc atctttaata
    25261 agttctccta aggaataact ggaagcaagt cggatagttt gatccgttgg tttatcaagt
    25321 agatctaacc ccaatgcttg tgcagattcg atgactgcgg tgttttttcc ttgccaagtt
    25381 gtcaataaat agctgatttt tcgctgacgg aataagagat ccatcccatc aatggtacgc
    25441 catgttcccc tgagcgcgta atagattgct tctgcagtat tttctccgcc atctgcaatc
    25501 ggcacattga tttttcttcc tttacgatct tcccagccat ttagaactgc ctgatttagt
    25561 tcaaatgaag aagcacttcc tttaaatgaa tcaattgccg ccaaaatatc catctgtatc
    25621 cctcactttc ttcttaagta agacactttt tttcattttt ttcaaacaaa acaatgctat
    25681 tttcttccat ttttctatat actaaaaggg aactcattga ataaaggaga gaaaaaacat
    25741 gaaaatcgca atagctgggg ccggagcaat gggcagccga atcggattga tgctacatca
    25801 aagcggaaat gaggtcctct tgatcgatcg ttggcctgct cacattgaag ccattcgtac
    25861 aaatgggttg attgctgact ttaacggtaa agaagtggtc gcaaaactgc caatctattc
    25921 accagaagaa atcatcgaaa gcaacgaaca cgtggacttg atcgtagcat taacaaaagc
    25981 caatcaatta gatgatatgt tttgttcgat ccaatccatt attactgaca atacttatgt
    26041 tttgtgttta ctaaatggat taggtcatga agatgtactg gaaaaatatg tgccaaagaa
    26101 aaatatctta tttgggatca cgatgtggac agctggtcta gctggtcccg gaaaagtcac
    26161 attgttaggt gatggagaaa tcgaactaga gaacttggaa ccagaaggcg aagcatttac
    26221 aaaaaaagtt gtagaagtct ttcaagaagc aaatttaaat ccgatctata gccataacgt
    26281 tcgttattcc atttggcgaa aagcttgtgt aaatggaaca ttgaatggtt tatgcacgat
    26341 tttggattgt aatattgctg aactaggcgc gcaaaaagcg gcagagagca tggttcgaac
    26401 aatcgtatct gaatttgctt ctatcgcagc aaaagaagga atcatactag atcaagaaga
    26461 agtgtaccaa catattgctt ctacttatga tcctgacaac atcgggttgc attacccatc
    26521 tatgtaccaa gacctgatca aaaaccatcg tttgactgaa atcgactaca tcaatggtgc
    26581 catttggcga aaaggacaaa aatatgatat tgctacacca tattgtgcct ttttgaccca
    26641 attagtccat gcaaaagaag gaatccttgg cgcagaataa aaggctctct gatgagaatc
    26701 agctgaatca gcagctggtt ctcttttttt aagcccaata aaagatcaaa aagaaaacac
    26761 aactctcatg atgccataag aattgtgctt gtttgtttca gtacataatc tcttcaaaat
    26821 gtaggttttt tatttgtttt caacagagga gaggtcatat tcgtattgct cataaatagt
    26881 ggaatcgtta gtgataaatc gttttgccca attttctgca tcataaaatt ccaatagctc
    26941 tgataggatt ggcttaaatg gtgtatgcag aaattccggt aattcatcta aatcacgttc
    27001 tcgaagcaaa aaaatatttt caggacgata aaaatgataa tgagcaatcg cttgattatc
    27061 cgtcactagt ttcttttgaa aatacatgga ttcaaatgtc cgcaaagtca ctccttgctg
    27121 accctcccgc aacagttcca gaatcccgcg cgattcccct gtttttttca ggtactcttc
    27181 ataagtaaga tatccaggtt gttcattccc tcgatgtgga agaatgaaat agttggtcgt
    27241 aacatctctt tttataaatt cctgcttata gctttctgct cgttcttttc tcccattatt
    27301 cgttgcacca aaaaatagat ccgtctttgg ttcgctagaa gaaaaattca tatgtttcga
    27361 ataaaatgaa ctattatgtt tcagcccaag ttcttttgct tccaaaaaat caaaatgata
    27421 aatctcatcg attgccggtt catcttgtgc ctgcttcagc atcgcctcgt gttctgcttt
    27481 gaagtgattc caaaaaaata acacaatttt tgcattggta aatgggcgca cgcgtcgagc
    27541 catctccata tcaaatccat aatctaaaaa aacgatcagc tgataatttg ccaactgttg
    27601 tctagctgat ttaatgaatt ccgactgtag taaatctttt ccggaccaaa catctaaaga
    27661 gcggatggaa ggcaaaaaat aaagtgattt attttttgca actagcagta cgtcattttt
    27721 gttcatcttt tccgcctcac tttctctgtc actttttcct atgatagcct gtttaaatag
    27781 taaaaagcca gtaaaacgtt tcctatattt gtatgaaaat ttaacatttg tatgaaaata
    27841 tgatgaaata aatcattctt ctattaaaat tcgcctaaaa gttttctgaa tagttgtaat
    27901 tgtctgaaaa tttgttatca ttagtcattg ttaaatattg ctatttattc ttaatataaa
    27961 ttttgattgt gaatttaaaa gaaagaggtc atttatggaa gataaaaaat tgaaaaaaca
    28021 acttagctcc agacatatca ccatgttagc tttaggtgga gcaatcggtg caggtctatt
    28081 taaaggaagt ggcgaagcga ttgggatcgc cggcccttct gtattgattg cttttttgat
    28141 tggtgggctg attttatata tcgtgatgaa aggtctggga aaaatcgttt taagcggtgg
    28201 cgacacgcat cacggattat ctggtttgat acgcccttat ctaggtgctc attctgctga
    28261 ttttactgat tgggtctact ggtctatgtg gattatcaat atcatcgctg aagctgttgc
    28321 agctgcttcc tttttgcagt tgtggttccc gcacattcca gcctgggtat ttgtttttat
    28381 gttggctgtt ttaactacga tcatcaatct ttattcggta cggttattcg cagaaacaga
    28441 atattggctg gcttttgcta aaatcagtgt cattatctta ctgatcattt ttgctactta
    28501 tcttgtcggt cagcaaatgt tgggcacagg cgttttccct acccttcagc aattgactga
    28561 ccatggtggg tttactcctc atggtatgaa gggaatcatc agttctttgc ttgtagttat
    28621 ctattcttac ggcggttctg aactgattgc tatcacggta agcgaagcag atgatcctaa
    28681 acgtgcgatt cctaaagcaa tcaaaggtgt catgggaaga atcatttcct tttacatcat
    28741 tcctttgttt ttgcttttga ttctttttcc ttggaatact ttagcaggca cgaatgtcag
    28801 tccttttgtg atggtatttg aaaaaatgaa tatcccattt gcagcggata tcgtcaattt
    28861 tgtgattgtc cttgctttgt tctcatctat caattcgggt gtgtatgcat cttcaagaat
    28921 tttgttcttc cgtttgaagg atcgaaaagg ttcctctaga aaactagctg ttttaaacaa
    28981 gcatcaagta ccgcaacgtg ctgttttatt ttgtaccagt gttctttatc ttggtgtagc
    29041 tctttcttat tttgtcggtg acaaattatt tggctatctg gcaggttccc tttcgtatac
    29101 agtattattg atctggatca ttatcagtgc ggcatccttt gtactggcac taagaaaagg
    29161 aacattatgg gataaaggaa tcagcttttt tgcattagct gtcttaggat tgattttcct
    29221 tggtatcctg ttcacgaact ccattggggt aactgtattg actggcctgc tttacctgtt
    29281 catttatttc agttatcaaa aaaagaatga tgcgttcgta ttgacgaatg aatgaaatcg
    29341 atagaaaatt ttattttatt tatgtggaat agatggagct attgaagaaa ggagttgtga
    29401 caaaagtctt gttgcaactc ctttttccga ataaacagtc tcatgacctc ttggtaaacg
    29461 gtgttcaaaa accctctgtg caaaataatt gattgttctc catgctcatg acatgataga
    29521 agaaaggaat gaaaaaaagg agtttgtttt aaatgaaaaa gtattggaag tttttgttga
    29581 tgatcggtgt ttcaacggta gttatgtttg gtatgatgta cttgaatgtc tatgcgctcg
    29641 atcatatctt cttcagtcgt acacgagtat tcatggcgtt gatgatgggt gcagtcatgg
    29701 cagtcatcat gctgttgttc atgtggaaaa tgtatgaaaa caaaaagata aatgttatga
    29761 tcttagccgc tagtcttctt gtttttgccg gttccttgtt tatggtacga agccaaacta
    29821 ctgtcaacga cacagcttgg atgaaggcca tgattcctca tcattctatc gctatcctgg
    29881 caagtaaacg agctgattta aaagatcctg aagtaaaaaa attagcggaa gaaatcattc
    29941 aggcacaaga agaagagatc gacaaaatga aaaaaatgat caaagaaatg gaatgaccat
    30001 tatccgtcca aataaaaaaa gaggatgttc cagaagttat cagcttcaag aaataagaag
    30061 aagctacttc tggaacgccg tgaataaggg gttgtgaaaa agctgccctg catcaagtaa
    30121 taagaacagc caaacaaaaa tagcttctcc tatttttcgt ttggttaggc ttattaccgc
    30181 agatgcaagc ttttgaacac cgtttattaa gaggttgtaa aatcagcgtt ctgacctgag
    30241 tattagagag acaaatggaa aaatagttcc tcatattttg ccattttttg agctaatacc
    30301 gagggtcagc tgattgaaca ccgtttatcg agaggttgtg aaaaagtcgc ttagtttcgc
    30361 gtattaagaa aaattttgtg aaatggcttt ccacattttt gtataaattt ggcttaatac
    30421 cgagaaactg gcttttgaat accgtttatt cgtaaaaggg attgtgacaa aagtcttgtc
    30481 acaatccctt tttatgtttt tattgttcta attgcgctgt cttgcttact gcttcttgcg
    30541 gtttcacaaa tttttttgag attccccaac ctgtgaaggc actgaccatc gaaaacacag
    30601 gagaaagcaa actgaaaaat acgaatggca agtaagtgag tgtaggaata ccaaatgtac
    30661 ttgctacgaa acttccggca attccccaag gcactaagta gttgattact gttccaccat
    30721 cttctagcgt tctacctaat acagttggat caaatcctgc tttttcataa gtttctttaa
    30781 atgcattacc aggcagaata acagatagga attgttcacc aataaaaata tttactccga
    30841 tacttgtcat caatgttgta aaaatcaaat ttggtgtgtt cgtcatcttt cttgatactc
    30901 gttccatcac ggttgagatc aagccaaatt cgatcaacaa tccacctaat gacaaagtta
    30961 gaataatcaa tgacacagta ggcatcatac tttcgattcc tccgcggcta agcagtgaat
    31021 cgatttgttg gtttccagta tttgaaacaa accctgattc gatgacccca gccaacttcg
    31081 ttaaggaagt ttgtgggttt tgaataaaaa tcattcctac tgctacagcg atattcaaca
    31141 aaatcgttaa aattgccggc acctttttcc atgcacacag taacattaaa aataaaggaa
    31201 tcagtgacca gattgaaata gtaaagtttg cttctagaac atcagtcact tcacgaatag
    31261 atgaaagact tgcggcttta cttgtgtgtc ctaaaatcat gaataaaaag aaagaaacaa
    31321 caaatgccgg gacagttgac cacatcatat gcttgatgtg tttgaacaaa tcggcatcaa
    31381 caatagctgc tgctagattt gttgattcgg ataatggaga catcttatct ccaaaaacgg
    31441 cacctgaaac aatagctcca acaaccagcg ctggatttaa gcctagtgtt gtcccaatac
    31501 cgaaaaatgc aatcccaatc gttgacataa cagtaaaggc actccctaca gcgctaccta
    31561 cgattgcaca cacaacaaat acagaaggga caaaccactg gacactgatc aatttgaacc
    31621 caatgaccat tagagttgga atgatccctg cttgtatcca tacagcaatc aatgaaccga
    31681 ctaaaataaa aataaagatt gggatgattc ctggtttgat gccattgatg attccttcgt
    31741 gcagccaatc tgtcgggtat ctttttatca ctgcatacaa catgaccatt ccgatcgctg
    31801 ctaagatcgt gatattaggt gagaccttta atccgacaac gccaatcgca atgcctaaaa
    31861 caataaccat taatacaagt gttgcttcta aaaaacttgg tgttcctttt tttcccatga
    31921 taatctctcc ttttttagtt gttcttatgt tctaaaaaag ttaagatatc ggattactcc
    31981 gccgcaattc atccgcgatt cctcctttat aaaaataaaa agacctgttt taaactaagg
    32041 acgaatgact ccgcggtacc accttaatta aaaacagatc tgtttttcac tttcaaactt
    32101 ttattcaaat gtatggactt tccatgtaat tcgtcaatta ggcttgatcg gttcacagcg
    32161 accaccgact ttctgaacaa aacacctaac cgctactgcg tgaaaagttg acttgtacga
    32221 agtttacctt ccataagatc atttgtcaat gaaaaagaaa atattttttc atttttctga
    32281 aaattatttt tataactttc acacacaagt ttatttttta ctagaaagta gcgccctctt
    32341 gtttttttct tgctactatt tcttgatgtt cttgcttgat tctttctaat agctttggct
    32401 ctttcaacag atcaagagct gtattggcga gtagttctgc tgcaatcgca atagaagcaa
    32461 ggcctttgtc acttttagca gcagctttga attcttctgt gtgtccggca atatatgtct
    32521 cagagattgc taccgtaggc tggattgttg gtatgacttg gctgacattt cctacatcag
    32581 aagaaccgag acttgttctt tgttccgttt ctatttcttc atctggaaca cctgcttctt
    32641 ttgcatgctg gaagaataag tcatcaaaag aaggagtaac gatgacatca tcaactgcat
    32701 tttggaacaa tccaaactca aaagtcgtgc ccgtacttat cgcagcacct cgtacgatat
    32761 tttccacttt ttcatatact ttatctaatg tttgccgatt tcctgcacgt aaatagaaac
    32821 gtccagcagc gtaatctgga atgacgttgg cagctactcc tccgtctgta atgatcccat
    32881 ggatattgac atcttttggc aattgcaagc gtagagcgtt gattccatta aacacttgga
    32941 tcaatgcttc caaagcattt actccttttt caggtgctgc tgctgcatgg gaagcgacac
    33001 cgtaaaattt gatgtcgact ggatcattcg ctaaagattc tgtcgttttc ccataacgaa
    33061 aagctggatg gacgcataac gctacatcga catcctcaaa aaaaccttct cgaacaaagc
    33121 tgcctttagc tgatccgttc tcgccccctt cttcccctgg ggtgccatat acacggattt
    33181 ctccaccagt ttcatcaatg aattgcttca ctatgctggc ggctaatacg gagtaagtac
    33241 caaacaaatt atgcccgcat gcatgaccaa tacctggtaa agcatcgaat tctgccaaaa
    33301 aggcaattgt gggaccgggt ttattggaaa tataacgtgc atcaaaggct gttcggtgtc
    33361 cagctacttc tttttctacc caaaaccttt ctttttctaa ttgtttaatc aacgctgctg
    33421 aggaataaaa ctcatagtta cttacttcag gatgttcgtg gatatctaga gcaagtgctt
    33481 gatattctgg taaacgatat tggattagat ccttcaattg gtttgttgag attgttgttg
    33541 tcataaaaaa ttcctcctat gctagtcatt tttcttattt tctcccgaat gtttcccatg
    33601 caggcacgct tgatcctttt gatgttttct cgattacttt gactgtgtct tctgtttgat
    33661 aagcgtctac tattttttga tacgttttat tgttttcatc ttcttttctt gcaacgatga
    33721 tattcacata aggacgtgca tggtcatcca ctggttctaa gaagatggca tcagaagcag
    33781 gattaaatcc agcatctaca gctacaccgc tattgatcac tgaaatgtct acatctgaca
    33841 aagcacgtgc tgtttgggaa gcatctaatt cagaaatgtc tagatttttt ctattctcgg
    33901 taatatcaga aacagtgggc gcttgtcctt ttgccttatc cactttgatc aatccagcag
    33961 tttgcaataa cagaagagct ctgccgccat ttgtaacatc attagggatt gctacttttg
    34021 ctccgtcttt gatatcagct acatccttca aagtgttaga ataaattccc agcggtgcat
    34081 tgacagtatt tccgatcgat actaaatcag ttccgtgttc tttattgtaa ttgtctaaaa
    34141 agaattgatg ttggaaagaa ttcaaatcaa tcgatccatc tgctaaagcc acatttggtt
    34201 gattgtaatc tgtaaatttc acgtattcca aattaatgtt ctcctttttc aaacggtctt
    34261 tcacgttgtc ccatacatct gtatcttctc caataatccc tagttttacg atagtttctt
    34321 ttttgtcagt tgtatctgct tttccttggc tacaccctgc caaccaaata attgctacaa
    34381 atgtaatgac tacaaaacga ataatttttt tcattttttc tcctccactt tcaatgtgta
    34441 atttttttaa tcagttgatt actgataaat tgacttaaaa aaactaagat tagaatcaat
    34501 aatgtagcca caatcgttac atctgtttgg aatcgattat accctctcga tatagcaaga
    34561 ttccctaatc cgcctgcacc tactgctcca gccatggtag tcagtccgat cacgttgatg
    34621 atagtcaacg ccgagacccg aatgatacca ggcaaacctt ctaacaaata cacacgtaaa
    34681 atgataccga ttggactggt tcccatcgct tctgccgctt cgatcacccc aggatcaact
    34741 tctagcagtg cgttctcgat ttgacgagca aaaaacggaa caactccaaa aatcaatggc
    34801 actaaagctg ctttcgcacc aatcgtcgtt ccaactaaga aacgagtagt caatgctaaa
    34861 agagaaagca agataatgaa agggatggaa cgaatgatat tgatcagctt atcaagtatt
    34921 tcaaatagca gacggttagc tagtaatcca ttagggcgtg tcactactaa cgtcacacct
    34981 aagatgattc caatcatccc gccaatgaat gctgtccaaa aagacatata tagtgtttgc
    35041 ttcgtcgctt cgacaaattc atccggaatc aaataaacat tgggcaaata tgttttaaag
    35101 aaatcagtca ttttatcttc ctccttcgat cactttgata cgactatcta aatcgttttt
    35161 ttgtaaaata tttattttta caccagattt gatcaaataa tccaatgctt ttttccgctg
    35221 tttggtttct ccagctaaag taacgatcaa actccctaac ggcgtttctt gtaataactc
    35281 tacattgcca tataaaatat tcgctgttac ttgatatttg ctgtacagat gagcgattag
    35341 aggttcgttc gtctgatttc caatataact taattccacc agccatttat tggcgatctg
    35401 atcggcaaaa gcagaatggg caatgatcgt ttctaatgct tggtcaagat gagtagctgt
    35461 tcgaataaaa tctttggtca attcttcttc aggattgctg aagatctgga tgctgctacc
    35521 tttttcaacg attcttccat cttccataac cgctaccttg ttgcagattt ctttgacaac
    35581 ttgcatctca tgagtgatca ggacgattgt taaacctaac tggcgattca atttttttaa
    35641 caatgcaagg atctgaaggg tcgttttcgg atcaagtgca ctggttgctt catcacataa
    35701 taatacctta ggttcattcg ccaaagctcg tgcgattgct actcgttgtt tttgtccccc
    35761 cgacagttgt ttaggataag cagatgcttt ttcttccaaa ccgacaagtt ccagtagttc
    35821 attcactttt tgacgacgtt cctgtttcga ttttccagaa tactttaaag aaaaatcaac
    35881 gttgtcaaaa atagttcggc tttccattag attgaaatgc tgaaagatca taccgatggc
    35941 ctttctttct tttcgcaatt ctttgggaga gagctgactc agattcgtct gattgacaat
    36001 gacttctcct gctgttggtt tctgcagcaa attcatcaca cgaactaacg tacttttccc
    36061 tgcacctgag taaccgacga ttccataaac atcgcctttt tcaatcgtta aatctacaga
    36121 atctactgcc tgtatctctt tttctttttg tttgaaacat acagaaacat ctcgcaattc
    36181 aatcattaaa attcctcctt atctaaaaaa agcgtcctca agaaaatgaa aatgtcattt
    36241 tcttgaggac gcttttttgc gtgttaccac ctcatgttca agctgtgttc acacacaact
    36301 cctcttcaag tacgatcata ctctagcaag atatcggtcg cagccgtcat cgccttacat
    36361 ctatgctcgg gatgaattgc tcgatggcca ttttccaata gtcgttcctt gcttcttctc
    36421 agctaccgaa gctctctgtc aaaggttctt ctatcgtact taccatttca atgcaaataa
    36481 tattcaaaaa aaaagtcctt ttactaacat aaactatgtc agtaaaagga cgacgtatcg
    36541 cggtaccacc ttttttcgga agtttccttc cctcaagccc aacatctcaa aaagagagat
    36601 tggcgttcgt tttaacggtc gaacagttcc gaatttgcct acttatcgac aaattattct
    36661 ccaaggccat tttcagcttt tcttacttga ctcttctcac cagccagagc ctctctgcaa
    36721 agtttccaaa cttactttcc ttttcaacga aatcgatatt ttgttgttgg gattaatcta
    36781 catgaggaaa actgaaaagt caagtacgaa attcaaatta ttttttctat atttattttt
    36841 ctgttttttt atttaacatt tcaataacag ttcgaagatc ttttccttcc gcaagaagtt
    36901 tcaatttttc cttttcttcg acggtataat ttcggacaaa actggaatca ggtatcaaaa
    36961 caacgcctaa tcctgctcgt gaagcagctg ttgctcctac taaagaatct tccactgcaa
    37021 aggctgaact agcttctaat cctgttcgtt ccaaagctgt cagataagga atcggtgaag
    37081 gcttgttctc cgacacttcg tctccgaatg taaacgtgtc caagtaatca gctaatttaa
    37141 agtgatccaa gatatctgtt gctcttttcg taacagtggt cgtagcaatg cctagtttca
    37201 tatgacgttc ttttaagtct gccaatattt cttttgcata tggttttaag tggatggctc
    37261 cgcttgtcag ctgttgataa aaatattctt cacgcttcgc tcggacttcc tgtacttttt
    37321 cagggctacc tgctgctttc actaaaatat caaaactttg tttccagttt tttccgcccc
    37381 aagcgtcaac gatttctttt gggatatgga tatcaaattg ctgaaatccc caaagccagc
    37441 catcacgata cgttttttct gtgtcaacta acaaaccatc taaatcaaat attgcacctt
    37501 tcatctatac attaatcctt tccaaaacaa tacacatagt ttagcacgac tggtccaaaa
    37561 agaacagaag caaagaacca gccttctcgt ttataagagt tcttggaatc gacggatata
    37621 gattttcttc acagctgtca caagcgccag ataagcgatg actgtagcaa ataaccataa
    37681 ccagtaattg ccaggaagtg gcatcaagcc taattcagca ccaaaacttg taaaaggaag
    37741 gacagaaccg acaataattc ctaatgtagt gataacaaac atggcaaatg ttgcgttgct
    37801 ttggataaat ggtactttcg gtgtacgcag tgcatggagc actaaggttt gtgtccatag
    37861 tgattcgaca aaccatcctg catggaataa tgcgatgaaa atcactcgct gtgaactatc
    37921 caatgtatgg aaacttccgc ccactacagc aggacaaata acaaaataca tcaacaagta
    37981 agtagtaata tcaaatatcg aacttgttgg accaaagtaa accatgaatt ccccaatact
    38041 agatgcattc catttttttg gctcgtccaa atattcttta tccatccgat cccatggcaa
    38101 ggaaatgcag gaaacatcat agatcagatt caaaaacaaa agttgtaaag gcagcattgg
    38161 taaaaatggc aggaacgtac ttgcaatcag cacggaaaac atattgccaa aattagaact
    38221 tgctgtagcc ttgatatatt tcatgatatt cccaaaagtt tctcgtccag aaagaaggcc
    38281 tctttccaaa atcgtcaaat ctttttctaa aaggatcacg tctgcagatt ctttcgcaat
    38341 atccacagct gtatctacag aaataccgat atcagccact ttcattgcag gagcgtcatt
    38401 gataccatct cctaaaaatc cgacagtatg accattttta cgtaaagctg tcgtcaaacg
    38461 tgctttttgc tgaggattca attttacgaa tacttcatgg tcttcagcta cttgtgctaa
    38521 ttcatgatca ctcatttccg agattttctc accggtaacc aattcattga tctctagacc
    38581 tacttgacgg cacactgaac gtgtaactaa ttcgttgtcc ccagtcagaa cttttacagc
    38641 tacaccatgt tctttcagag ctttcaacgc ttgtttcgtt gtttcttttg gtggatcaag
    38701 gaaagcaaga taaccaatca aaaccatgtc tgactcatct ttgactgaaa attctccaac
    38761 gacgcttgga ttggtctttt gtgcgacacc aatcactcgt aagccatctt cattaagtga
    38821 ctgtactttt gcaagtacgg cttcttttcg ttcttttgtt aatggtgtaa cagtaccgcc
    38881 aagatcaata aatttagaaa tgttcagcat ttcttcaatc gcgccttttg tgatcatttg
    38941 tgttttacca gctggatcag caaccacaac actcatacga cgacgttcaa aatcaaaagg
    39001 aatttcatcc actttttgat aattgatctt atctgtatct aaagtttctg ttgcttcatc
    39061 aatgatagcc acatctaaca aatttttcag ccctgtttga tagtagctat ttagatacgc
    39121 atggcgcaaa acacgatcat cttcatttcc ttcacaatcc atatgatatt caagaatgat
    39181 tttatcttgt gtcaatgtcc ccgttttatc cgtacataac acatcgattg caccaaagtt
    39241 ctggatggca ttcaaatttt tgatcactgt tccttttttg gccatcacac ttgctccttt
    39301 gaccaaattc gtcgtaacga tcattggaag catttcaggt gttagaccaa ctgctacaga
    39361 tagtccaaaa agaaaagctt ctagccaatc cccttttgta agcccattga tgacgataac
    39421 tgctggagcc atcaatgaca taaatttgat cagtagcatg gatgttttat ggatgcctaa
    39481 ttcaaagctt gattgaattg gtttttcaga tagcgtttct gctacatgac caaatagtgt
    39541 ttcatttcca acagaaacaa tgattccttc cgcacttcca ctgatgacat tacttcccat
    39601 aaagacaaga ttttcgtaac ttgtttcgct ttgctgatcg tcttcacata ctttcgcatg
    39661 cttttctact ggatagcttt cccctgtcaa tgctgcttgg gaaatgaata aatcctttgc
    39721 ggacaataaa cgcatatccg ctggtatcat atcccctgct gaaagccgaa caagatctcc
    39781 acagacgatt tcttccatag gaatctctgt gtactttcca ttacgtttta cagctgccgt
    39841 tactttcaca agtgatttca gtttttcagc cgcttgattg gaacgtacgg actgaatcaa
    39901 cgtcattgtc ccgctgacga taaccatagt gaagataatg gctacaccga aaagatcttt
    39961 atctccagga gctgctaata catattccgt ggtgaatgaa ataattccta atgcgatcaa
    40021 cactaatgta aatggcgtga tgtatgcttt caatatttct acaatgaatg gtgttttttt
    40081 gccatatgaa atcgcatttt caccgtaatt ttctcttgaa cgttcgattt gttcttctgt
    40141 caatccattt gctgatgcat ggaatagttc aaataattct tgtttcgttt ttccagcaaa
    40201 agctgcatag attttttgtg tatctttctt tttttccatc tcaatctctc ctttgtgtcc
    40261 aaaagacagc tgttctcgct gcttttggtc tttagctatt catcggatcg atagagaaaa
    40321 aactgaaagg ggctgagaac agatagataa tagccctggc ctttcctcta gatccttgtt
    40381 tttcgaatga ctgtcatcca tttgttctca tctcctttac taagtaattt taattcatga
    40441 cttccaatca tttttcgaat agttattttt ccaattagac cacctccttt ttatttttca
    40501 ccgatgggga tttgcggcaa caaaaaaacc tctcgccaaa tactcagcga aaggtttgaa
    40561 aaagcatatt aaaaaaagct tctcagatct catcgttgag ttttgacact gtacaacgta
    40621 aaaagcagat acctgctttt agctacctta tgaaaagcct tatctcgaca attcctgttc
    40681 tacccattgg cgtctctcga cgtttttggg cagtagccta tgtttgtata ggagcctcac
    40741 ctaacaaatc ttattcggtt ttccttcgtt aatataacaa ctaaaaaaga ggatgtcaaa
    40801 tcaattttcc atgtttttca tacttttttt aaaatacaca cgttatattc ctgtctcacg
    40861 cggttttatt tgtctcattt tccacctccc gctataatag aaatatagta ggaaacgagg
    40921 tgctgttatg gaaagaacac ttgttattat caagccagac ggcgtgagac gccatttagt
    40981 cggaagcatc atccaacgtt ttgaagcaaa gggactggcc attgctgaaa tgaagtttga
    41041 aactatgact cctgaattag caaaagaaca ttatcagcac ttgactgagc gcagtttttt
    41101 tgatgaactg atcgattaca tgacttccgg tcctgtcgtt tatcttgtgc tggtcggcga
    41161 ggaagtgatc gatatcgtcc gtaagatggt tggagcaaca aaagctgccg atgctgttcc
    41221 aggtacgatc cgaggagact acgcgctgcc ggggactgaa aacatcattc acgcttctga
    41281 ttcccgtgat gctgcggtaa aagagatcgc ccgctttttc ccgccattcg atataaacaa
    41341 accgtctgaa aaagcggcga agtgatacaa attctaaact gctggtctag ttgctgggtt
    41401 cagcaatggc cagcagtttt ttctctaagg acctccgttg attctttcct aacatatatt
    41461 cgagcactgt gaagaattct tggtagtctt tcgtcttttc ttcccctaaa gcagcagaaa
    41521 tcaatctttg ataataatct gccatcaatg tttcatagat cactgtactt ctttctttat
    41581 gtgctaagct ccgtttcata tcttcattca cactacgtgc ctttttcaac cgaccatttt
    41641 ctatataaaa cgaagtcagg ttcattaaaa aagtaaagag atactgctca tgattgttca
    41701 actgtgctaa tcgtcgcatt ctttttttga ttttcaaata attgttctca atgaaccgat
    41761 cactgaatat ccaaggaaaa tcagctagaa tagatagttc aaattgacca tagtctggta
    41821 tctgcatcaa gtattcttca atgattcggc attcataacg taaaaattca aattctgtct
    41881 cagatacttc tgttttttcg accatgtgaa gattcaaaac caaataatac agatcttctg
    41941 ttttcttaaa atgcctacgg ttattgctga tctctttata aggatcaaag ctgtcttctt
    42001 gtacccgatc tagcattcgt ctttttttct ttaactttat tggttcatcc agctggtttc
    42061 gcatccattc atattctgca agtgtcatgt tcattcgctc taaaaatgat ctcaatgtta
    42121 caaatgacat ttttttcact gaatttgtct caatcccgac tagagaagtc ttaaaacaga
    42181 ttccttccgc taattgggac tgtgtcaact tcttgttgac tcgaagtttc ttgatcaatt
    42241 caccatcata cataatagtt ctccttttta atttaaattt ttccacattc aataaataga
    42301 tacgttaaaa aggaaaaata cacgaaaaaa ataaaaaaat gtttttttca taaaaaatac
    42361 caaccttcaa gcattagaat gttgtctaac tactgaaggt tggtttgatt gtttattgat
    42421 tatttttttg ctgttcaacg tctgttcttg ttactggata taagttggtt gttgttttat
    42481 taccattttt tgaaaagaca cttgtcgaat gttcgccttt ttgcttttcg attgcttcat
    42541 tacgttccgc ttcattttta tagtcatatt ttttcggatc aatcgtctca aatccgtttg
    42601 gcacataaaa ccgcaagaga ttttcctgat tcagcttatc tgaaagactt aacgcagtct
    42661 tgactttttc ctgatctttg gagatttcct catcgattcc tgctgctttc gtatcgatga
    42721 tttctcctgt ctgattattg taagcttttc ctcctaggac ggtgtatttg ggtgtcacaa
    42781 agttcccatt tcgaaaagca actacttgat catgctgaga agataataag tctgtaccaa
    42841 aatgaagata atctttatca tcgattccca atagatgtag taatgtcggc aaaacatcga
    42901 tttctccacc gtattcatga ttgactgtcc ctttagtata accaggcata tggatcatca
    42961 atggcactcg ttgcatctgt gcgttgtcaa attcatccca tgtatctgga tctttaccaa
    43021 gagcgcttgc taaatcttta ttatctgtat tcgagatccc aaaatgatcc ccgtaaatca
    43081 caaagattga attttggtaa ataccagatt ttttcaaata cgtaaaaaat tgttccaatg
    43141 attgatccag ataatgtgcc gttcgaacat aattatctac tgtgctattg ccagtattca
    43201 aagatggaaa gtcgaaattc ttgtcatccg tataataagg cgtgtggttg gttacggaaa
    43261 gaaatttcgt gtaaaacggc tgttgtagat gctcaagata ttgggcagat tctctgaata
    43321 aatctttatc taatatccca taacctaacg tttcgtcgga ttcatcaaaa taagatcgat
    43381 caaagaaatt gtcataacct aagtttttat atacatgatc tctattccaa aaactagcta
    43441 cattcccatg gaaaacagcg cttgtatagc cttgcttttg tccaaggatc gctggtgcgg
    43501 cttgaaagac attgtctgac ccaagttctg tgaataatga gccttgaggt aaaccaaatg
    43561 tgccagtttc caacatattt tcagcatcac ttgtctttcc ctgtcctact tcatgaaaga
    43621 aattgtcaaa gctgatcgtt gcttgattct ggaaaatact atttaaaaac ggtgtgactt
    43681 cttgtccgtc taccttcata ttaatcaaaa actgctggaa actttctaaa tggagaacga
    43741 tcacattttt ccctttagcg atcccaaatg tttcgggatt tggctctgca taatgtttct
    43801 ttgtataatc taaaacttca tcaatcccat tactgcttgc atgcgcgcga acactgctag
    43861 tcattcctgt tttgattccg tcataaatag tatacgcatc gattcctaga tatttgacaa
    43921 tatatgaacg gtcaaatgtt cgctgcagca attgtggacg gttggcttcg ctcagcgcta
    43981 gatttcctga aaaaataagc cctgaaagac aggtaacagc gatagccatc ggtttgccga
    44041 cagggttgct tttgataggt attttcttga ccttcaacca aaccagtagc ccaatgaaca
    44101 cagcgatatc cagccagtag atgacatcat ggggcttcat caaagaaaag gagcttcctg
    44161 acaatccttg cgacactttc gaaaagccta atacagattt gactgtaatg aaatctgtga
    44221 attcccgata aaaaataata ttcaagtaca acaaaagcgt atttaaaata tcaatgatca
    44281 aaagaatagc taacgctggc tttggctttt taatatacaa agaaaggcca aaaaacaata
    44341 aggtagtcgc aattgggttg atccacaaga taaagtactg gatcgtacct tccacaccta
    44401 atgaaaaatc tgcatagtag gcaatgatcg tcttcagcca gaaaaagaaa acagctgtca
    44461 aaacgattgc taaacgattt tggcacaatt ttttaagttg tgtcacgtat atcacttctc
    44521 tctattcaca ttttttctta ttttgctcct gaaaaatgaa aatttcctaa aaaagttgtt
    44581 atcttaagat ttttttattc tttctaaaga ttattccgtc cacttttcac agcataaaac
    44641 cgctgcgatg taagcgatta aactaatgaa gagataaaag tcttgtggtg accgcttcac
    44701 tccacggtat tcaaaagcca gtttctcggt attcgtacaa aaaaagaggc tgtgacaaaa
    44761 ttcttgtcgc agctccttat ttcttgatgc aggacagctt tttcacaact tcttccttaa
    44821 acggtgttct cctgccaact cctcggccac aagtcacaat atttataaaa catgagaagc
    44881 tgttttttga aattgttcct tgtggctcag agttaaacgg caggctcaag acctcttgat
    44941 acacggtgtt caatcagctg accctcggta ttagattatg tcaggacgct aattttacaa
    45001 cctcttggta aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa
    45061 aatataagga gctatttttt gaaattgctc cttgtggctc agagttaaac ggcaggctca
    45121 agacctcttg ata
//
LOCUS       DACECY010000020        43584 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.021, whole genome shotgun sequence.
ACCESSION   DACECY010000020 DACECY010000000
VERSION     DACECY010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.021"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            160..840
                     /locus_tag="IWO38_001122"
     CDS             160..840
                     /locus_tag="IWO38_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0313222.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            930..1964
                     /locus_tag="IWO38_001123"
     CDS             930..1964
                     /locus_tag="IWO38_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ0313223.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1978..2313
                     /locus_tag="IWO38_001124"
     CDS             1978..2313
                     /locus_tag="IWO38_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313224.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2297..4777
                     /locus_tag="IWO38_001125"
     CDS             2297..4777
                     /locus_tag="IWO38_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313225.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4833..6392
                     /locus_tag="IWO38_001126"
     CDS             4833..6392
                     /locus_tag="IWO38_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0313226.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6389..7753
                     /locus_tag="IWO38_001127"
     CDS             6389..7753
                     /locus_tag="IWO38_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0313227.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7924..8994
                     /locus_tag="IWO38_001128"
     CDS             7924..8994
                     /locus_tag="IWO38_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0313228.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9009..9677
                     /locus_tag="IWO38_001129"
     CDS             9009..9677
                     /locus_tag="IWO38_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0313229.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9697..10275
                     /locus_tag="IWO38_001130"
     CDS             9697..10275
                     /locus_tag="IWO38_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0313230.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10676..11587
                     /locus_tag="IWO38_001131"
     CDS             10676..11587
                     /locus_tag="IWO38_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0313231.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11965..12882)
                     /locus_tag="IWO38_001132"
     CDS             complement(11965..12882)
                     /locus_tag="IWO38_001132"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ0313232.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            13084..13521
                     /gene="zapA"
                     /locus_tag="IWO38_001133"
     CDS             13084..13521
                     /gene="zapA"
                     /locus_tag="IWO38_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ0313233.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13579..14127
                     /locus_tag="IWO38_001134"
     CDS             13579..14127
                     /locus_tag="IWO38_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ0313234.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14242..16602
                     /locus_tag="IWO38_001135"
     CDS             14242..16602
                     /locus_tag="IWO38_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ0313235.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16689..17003
                     /gene="trxA"
                     /locus_tag="IWO38_001136"
     CDS             16689..17003
                     /gene="trxA"
                     /locus_tag="IWO38_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ0313236.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17185..18963
                     /gene="uvrC"
                     /locus_tag="IWO38_001137"
     CDS             17185..18963
                     /gene="uvrC"
                     /locus_tag="IWO38_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ0313237.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19108..20007
                     /locus_tag="IWO38_001138"
     CDS             19108..20007
                     /locus_tag="IWO38_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0313238.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            20000..21262
                     /locus_tag="IWO38_001139"
     CDS             20000..21262
                     /locus_tag="IWO38_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0313239.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21423..23321
                     /locus_tag="IWO38_001140"
     CDS             21423..23321
                     /locus_tag="IWO38_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ0313240.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23651..24370)
                     /locus_tag="IWO38_001141"
     CDS             complement(23651..24370)
                     /locus_tag="IWO38_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ0313241.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            24707..25039
                     /locus_tag="IWO38_001142"
     CDS             24707..25039
                     /locus_tag="IWO38_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313242.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            25026..27029
                     /locus_tag="IWO38_001143"
     CDS             25026..27029
                     /locus_tag="IWO38_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ0313243.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            27048..27518
                     /locus_tag="IWO38_001144"
     CDS             27048..27518
                     /locus_tag="IWO38_001144"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ0313244.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27539..28123
                     /locus_tag="IWO38_001145"
     CDS             27539..28123
                     /locus_tag="IWO38_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ0313245.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28138..29124
                     /locus_tag="IWO38_001146"
     CDS             28138..29124
                     /locus_tag="IWO38_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ0313246.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            29114..29425
                     /locus_tag="IWO38_001147"
     CDS             29114..29425
                     /locus_tag="IWO38_001147"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ0313247.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29447..31228
                     /locus_tag="IWO38_001148"
     CDS             29447..31228
                     /locus_tag="IWO38_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ0313248.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31221..32597
                     /locus_tag="IWO38_001149"
     CDS             31221..32597
                     /locus_tag="IWO38_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ0313249.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32604..33239
                     /locus_tag="IWO38_001150"
     CDS             32604..33239
                     /locus_tag="IWO38_001150"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ0313250.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33269..34624
                     /locus_tag="IWO38_001151"
     CDS             33269..34624
                     /locus_tag="IWO38_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ0313251.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34816..36210)
                     /locus_tag="IWO38_001152"
     CDS             complement(34816..36210)
                     /locus_tag="IWO38_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ0313252.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            36302..37273
                     /locus_tag="IWO38_001153"
     CDS             36302..37273
                     /locus_tag="IWO38_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ0313253.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            37270..38052
                     /locus_tag="IWO38_001154"
     CDS             37270..38052
                     /locus_tag="IWO38_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ0313254.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(38221..38592)
                     /locus_tag="IWO38_001155"
     CDS             complement(38221..38592)
                     /locus_tag="IWO38_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0313255.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            38726..39169
                     /locus_tag="IWO38_001156"
     CDS             38726..39169
                     /locus_tag="IWO38_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ0313256.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="IWO38_001157"
     CDS             complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="IWO38_001157"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ0313257.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(40040..41407)
                     /locus_tag="IWO38_001158"
     CDS             complement(40040..41407)
                     /locus_tag="IWO38_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0313258.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41391..42221)
                     /locus_tag="IWO38_001159"
     CDS             complement(41391..42221)
                     /locus_tag="IWO38_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ0313259.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42384..42821
                     /locus_tag="IWO38_001160"
     CDS             42384..42821
                     /locus_tag="IWO38_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ0313260.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            42897..43517
                     /locus_tag="IWO38_001161"
     CDS             42897..43517
                     /locus_tag="IWO38_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ0313261.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        14277 a         7488 c         9251 g        12568 t
ORIGIN      
        1 ttaataaaaa aggaagaata ttcatgtaaa cgattttatt gtttttttct cttttataca
       61 aaaagggtca taatttcaag taagcggatg aattcatgag gattttttgc atactgtctc
      121 tactatactt aggtcactaa gaaaaaggag tgatttatta tgaaaaagaa catgattggg
      181 acattattac tttcaacact tcttctaggc ggcttggcaa caccagcttt tgctgagggt
      241 caagcgactt caaaaggaga tattaccttt actgaaccaa caaataccgt ggagcctctt
      301 aaccctactg atccttcaaa accagtagag ccagcagatc cggaaaatcc agcaactggc
      361 cagacaggtc ctttaacact ggatgtagtt cccgaattgc catttggtac acacgagatt
      421 gaaagcggaa caaaaacgta tcaagtagac gcttcaaaaa acgatacacc ttatctccaa
      481 gtatcagatc gtcgaggtgt aggtgcagat ggccaagcac aagggtggaa cgtgacagtt
      541 tcggtttcag attttgtaaa cggttcacaa gttttgcaag gcgctgaatt agattttgga
      601 acatccactg ttaaaagtac gtcagataac gaatccactg gaccaactag tcaaactgta
      661 actgggttga gtaaagcaag tgcagcgaca ccaatattta cagcagcaaa agatcaaggt
      721 ctgggaacat ggctttctgt ttatgatcca gcaaatatca cactgaaagt gccgaaagca
      781 gcagctggaa cattcacggc tgatttgact tggaacctcg tggctggccc agtagcatag
      841 agtatgaaat aaattcaaca aactgcggtg aaaatactat aggtgaagga ttttttaccg
      901 cagttgttgt tgaaaggatg tggtggacaa tgaatcaaag taaacgactg tttgtttcgt
      961 ttttttctat tttaagtcta tttttcattt ttccgtcaat cagccaagca aaggattcag
     1021 caggagactt tggaatcaaa ccggtatttc cagagaacca aatagataaa gcaatcggtt
     1081 attttgattt attagtggca ccggaacaaa atcagacact agaagtaatt atcagcaatt
     1141 cttcagatga agaacgaaca tttgaggtct ctgtcaatcc ggcggtaacg agtgatggtg
     1201 gaacgatcga ttatagtcaa aaaaacccaa cgttagatga gacattgccg tttgatgtcc
     1261 gtgacgtttt gctcattgcg aaaaaggaaa tcaatgtatc agcacatgca gaaacaactg
     1321 tacctataga agtgaaaatc ccagcaaaaa gctttaaggg acgtgtatta gcgggtattc
     1381 atgtttcacc taaagaagaa gctgaaacag aaaatgctac agaaggagca caaatcaaaa
     1441 atcgtattgc ttataatctc gcagtcgttt tacaagaaag tcaagagacg atagaaccgg
     1501 atctgaaact tttatcagga gatttggacg aggtcaacgc aaagccaact gttcagcttc
     1561 gtttccaaaa tcctcagcct aggattatca gcaatttgat atttacgagc aagatctttt
     1621 atgaaaatca gctttatatt gaaaatacgt ctaatgcatt tttagttgca ccaaattcga
     1681 attttcactt gaatcttgat ttagctggtg ataaagcaaa agccggcgat taccgcgcag
     1741 aaatcatagc aaaatcaggc gacagtaatg aatggcgatt tacacaaaac tttacgatca
     1801 aaaaagaaaa agcacaaaaa gtaaatgaaa attccgtttt tgcagtacaa gaacaatctt
     1861 tcccatggct ttatgcagga ttggttgctg cagttctagt gttatggttg attttgctgt
     1921 ttttgtgggt ccacaagaaa aagaaaaaag gagaaacaac atgatgaggt gatttcgttg
     1981 aaaacatcga tcttattggg agggattatt gcaagtttgc tgttcttcag ctggcaagag
     2041 ccagtccaag cagataccaa aagttcaggt acgattacat tttatgatct aagtccaatt
     2101 ccgcatccaa gtggttcagt caatggcgca tcaaaacagc agacacagct accgatttat
     2161 aaagaatcta atcaacatgg cgtattcccg gagacgaatg aacaaaagat ccgttttctt
     2221 tatttgatca ttggtataga attatggctt cttgcgtggt tgttgttatg gagaagaaag
     2281 gaaaaggagg atgaacatgt tgctacaaaa taaattttca ttttttcgct ccttcttttt
     2341 tacttcgctt tttgttttaa gtatatttat cttttcacct tcggcagaag ctgttggagc
     2401 aacgcctcct gccatttctt atgatccata cacgtatgaa aatatcgtac ctccaaatgg
     2461 tggacgtagt gcagtcaatg gcgggaagct tgtccaacta actggggcta accaattgcc
     2521 taaattgact gctacttatt tgaacgatgg ttcaaatgat ccttggaaag gtggcacggt
     2581 ccgttttcat tgggaaagcc atgtgaaact aacaaatgta cttgatttgg tgaatatcac
     2641 tgcacaagga tttgcatttg tggcgatcat caatttgccg gaacatgtat caagcagtga
     2701 tgtattaaaa gcaattgact ggaacaacgc ctacttgtat attgccggtg cccccatcaa
     2761 ggttactagc ggaagtttat tatcagcagg aagccatacc ttgcgttttg ggatggggtc
     2821 gtgggataca aatactctat tgacagatat tattgctatt attacgcgag gcgaattcaa
     2881 cgtggacgat atcccgcttc aatttgatgt tacagctaat gtcgcgaaga tgacagaaaa
     2941 cggagataaa tatgatacga gtccaaacaa aattttgacc aagggtaaat tacaaccttc
     3001 tatgtctaaa aaggccgttt tttctgttga tttttatgat tcgaataata ttatccaaga
     3061 tcgtatgttc aattcgattt tagctccagc tgcaggtaga ttttatccta aattaactgg
     3121 cggaaacttg aatccaggag catatgcatt ttctgcaagt gcagagataa atacgtggaa
     3181 tagttacatc tcaccaagtg attcaacagg aaaatataat acatttgctg ataccacaga
     3241 atacgtggat ggaaataacc agaaccttcc aggctctacc aatagtcgga cattgacgat
     3301 ggggctgact gaaggacgtt ttgatcagtt gccatttagt cgctttgatc gcgtagtcaa
     3361 ctattttaca ggaaaaaacg ttacacaagg agcaatactg actcatactc cagcaagcac
     3421 aagtggattg aatcagacag ccaatatcgt gtacagtggg aaagatgcgc aaggcggatt
     3481 gctttctcct gttgctttaa aggtattgga gcagtacaag ctagacggaa cggttttacc
     3541 aacaaactta acagccgata gtcagtgggg aaagctcctt tcttctgcat cgtaccaagt
     3601 cacggccaat tggtcagcaa ctgaactaaa aaagggttcg atccattaca ggctttattc
     3661 gaaaggaacc caaaaattag cgacaggatg gacagatcaa cttttccaaa cgatccaatc
     3721 aaataacggg ggtgtaaata cagctacttc tacactacct gcattggctt cagggcaata
     3781 ttattttgat tatcgcttgg tggacgatgt acttactaac atgtatccgt cttttgcgta
     3841 caaatggcag tctgaacaag ctggaatcac cactcttcct caaataacag tggctaattt
     3901 tccatctgtg agtgcagata gtcagctaaa aaaccttagc agacaacctg agacaggaga
     3961 tcccctgtct gctctatcag gagatacgat tcaagaaaat gtgacattca cgttaacaaa
     4021 atctggggat gcgataaccg acaaaaaaat cacgatctca cttcctgaaa atactactta
     4081 tatcaatggt tccttaaaat taaatggtac aactatcgca gataccggca ttcaacaagg
     4141 agtttctgtc ccagcagatc tattgagcaa aataggagat accattcatc ttacttacaa
     4201 ctatcaatta aatactgtag atacttcttt acaatccgtc agcatactga caaaggcagc
     4261 tgtactttcc agcaatatta cattagcaga tggtgctaag cttcctaacc cagttgtcca
     4321 aacatctgct aaaacaattc ttgtccccaa acaagaatta acattagtta atgtaccaga
     4381 tgattttaca tttggtaacg atctccccaa accgttgaag acaagttatt atgaatcaaa
     4441 aggcgatttt tcttttgatg tgcgggatac tcggcttcca agcacgtctc cttggcaact
     4501 gacaggtaca ttgacttctt tatttaagaa tgatcaaggt caagaattaa gcggaacaaa
     4561 attatacttc aatcattctg gatcaaagca attgattcag caaggtcaaa acacgttgat
     4621 ttatgagagt gatggaacag ctaaaggaga ggttctcgtt gactttcccg ataccgatgg
     4681 tttactctta gaggtaaata gcagtacgaa tgctcaacct ggcgcaacgt atcaaggcat
     4741 ggtcacatgg gaactgacag ctgggccaac cagctaagtg attagaattt gtatggatct
     4801 gtgataactg tcacagatcc tctttttcaa ggatgagaag gaaacttctg ctgttaaaat
     4861 cagaaatgct tgaaggagtg gacttggaac ttttcagttt gcttgaaaaa aatgaacaac
     4921 ggcaaatcaa acttttccaa cttttattag aggaaaaagg agaggtttcc gttaataacg
     4981 tacttacccg cttatcagtt gatcgggcta ccttgaaaga agatctgttt tatttacaag
     5041 ctaacttgga aaatatcaaa caattggaaa ttgttatcca agaagggaat attttattaa
     5101 tcagacatgg aaatatagca gcagtcgatg tttattatca ttgttacttg cgctattcta
     5161 caaaatatca gatacttctt tatctgttgg aaaatggctc atttgatcgg cagaaacttg
     5221 tcatggaact taatatcagt tcggctactt tgtctcgcag agtgaaggaa ttaaatgatt
     5281 ctttaaatga gtttcatctg aaaataaaaa gcgggcggct gaccggtcca gaaagtcaaa
     5341 ttcgttattt ttatttccaa ttattttggt ttggacgtcc ctatcttgaa aacaaaaaag
     5401 aatacagtga tttatcagga aacacttttt tagatctgct gcaaaaagag tttagttttg
     5461 ctattacaga agaaggacga ttgaaatttg aaatatggct ttccattatg aagaagcgaa
     5521 tgcgctgtac gaaaaaatcc aaaccagatt atagcaatct tcctgttgga ttgtttcaaa
     5581 cgacacaatt tttattggac ctccatacat ttttgacccg ttacttcttt caatatgcct
     5641 ttccttggaa tcaaaatgaa acgttggttt tttatctttt catgatcagt aactttacat
     5701 tagatacaca taactcgcat gtccaggagt ttttgataag cgaaaaatac aaagacgacg
     5761 tagtagctca attgaatcga acgtttaaac aatcgataga agaatatttc tctttttatg
     5821 tttttccaga agagatttcg caaaagattg cgttgacagt gatgcaaaat cacgctcatt
     5881 ttgcctattt tagaggatgg atgaatattt ttggccaaga aggattaaaa cagcggttaa
     5941 ctgaagtaga agataaacaa ttcctggttt tatgtgaaag attggtcaga caaacatttg
     6001 ctattgttga ttttactgaa gtagacgagg aaaatgcccg ctttgaatta tttgctcgct
     6061 atgcatctat tttacggacg ctgatcaagc agttagaatt acgtctgcaa attgcttatg
     6121 atcttcctta tgaaaggcta atggcggata tcgtgaaaga aatcatccag gaacaattgc
     6181 tgaatacgat caaatatgat ttagtcgaat acgagaaaga aaagcagtat gatatcattt
     6241 tgactagcca attgaaagag tacccgtcac agaagtccgc taaagttttt gtattcacta
     6301 gcaacgaata taattatgat ttcccctatt tgaaccaatt tttaaaagaa tgttatttag
     6361 aaaaattgaa tttaaggatg aataaaacat gaaaatcgtg atcattggtg cttcccatga
     6421 aggcatcaca gctgccctaa atctaagaaa acttcaaccg aaagcagaag ttttactgat
     6481 cgatgaagat cataaagatg gactaggcta tgtttcaaat ggaatcaacc tttatttaaa
     6541 aggaaagata cgtagtttag ctgaagttgc ccataacatg cggactcttc aagaaagtgg
     6601 ggcaaaactg atcaccgaat ggagagtaac tgaacttgat ccagacaaac atcagctcat
     6661 cctcgcatcc aaagaaggaa aaaacgagac cataacctat gataaattaa tcgtagcgac
     6721 aggttcctct cctgtcacct tgtataaaca aattgaagct gaaaatgtct acacctataa
     6781 aaatctagtg caatctaagc aagtacttgc cgctttaaaa gaagcaaaag aagtagtgat
     6841 cttcggtgct ggttacattg gattggaatt agcagatgct ttgagaaata aaggatacat
     6901 tattcatcta gttgattata tgccgaatgt tttatcaaga tatttcgata aggatatgat
     6961 caacagtttt caaaatcagc tgcaaactaa gcaaataaat ttttatccaa atgaattctt
     7021 gatcgattgg aaaaaatcag aagagaaagt agtatccgta caacttctgt ctcaaacaat
     7081 aaaggcagat atggtcattt tttctgccca aactcgtcct aatacgacat tgttaaaaga
     7141 aaaagtggct ttgtatgagg atgagactgt gatagtcaat gaatatctgc agacaagtga
     7201 tccggacatc tatgcgattg gagatatcgt cccagtcagt tttgacaaaa ataaacggca
     7261 tctcttttta cctttagtta ctcgagctgt acatatggca agagcagtag cgctaacttt
     7321 gagtggtcag ccaaccgctt acgacctcag acaaaagata acagcagcag tcattataga
     7381 ctacttttta ggaactgtag ggctgacaga agatgaagcg ccattcttgg aacaaaatac
     7441 cggttcgtgt tcaggagaat ttgatctatt tccacaatac gacgaagaaa acaaaacagt
     7501 aaatgcaaag ctgatctacc atccggatac attagaaata attggcggac agctgataag
     7561 ccaagaattc ttattatcag atttgaacct gcttgcggat atcgtgaaac acaaaactac
     7621 gattccacag ctagcagttg aggattttgg atttctgtct gaatacacct ctcgttttca
     7681 ctacctcaat gagttagctt ttaaagtact agctaagaga gcaaatagca atagagtaga
     7741 taaaagactg tgagaaatat cacggtcttt tattgtgtgg ggatataata aatattaatc
     7801 aatcgagaat aaaaaattca tataatgaaa tagcggaaga attttttgag cataatattc
     7861 gccaaaaatt tatcaagtga taaaattgtt gtataaaatg attatttcag gaggaggagt
     7921 taaatgtttt tagcattaaa tgaaattatg cattcaaagt tacgttatgc gttagttgca
     7981 ggggtgatgt ttttgatcgc ctacctggta ttttttttga ctgggttagc ttatggctta
     8041 gctcaagata atcgaacagc agtcgacaag tgggaggcag attcgattgt gctttcgaaa
     8101 gatgcaaatt caaatctagg gatgtccatg attactaaga agatagcaga agaagtcgaa
     8161 ggtgggaaag tggcgtatct tgcccaaaca cctggagtcg tgactagtaa agacagtaca
     8221 gaagaaggaa aaatcaatgt tagcttcttc ggtatcgata aaaatcaatt catcatgccc
     8281 aatcttgttg aaggaaaagc ttttgataac gatgatgagg cagtaggcga tattagtttg
     8341 aaagaagaat atggtctagc cataggagat acagttaagt tatctggtag tgacaagacg
     8401 tttaaactaa ctggctttac ggatcatgcc aaattcaatg tctcaccagt tctctataca
     8461 accatcaatg cttaccaaga aatccgcttc gaaaaagaag atacaagtga aaatgcacgg
     8521 attaacgcaa tagttgtcag aggtaaaatc agtaatttac ctgaagattt agaacagatc
     8581 aaaatttcta aattcatcaa tgaattacct ggatataatg cgcaagtatt gacttttgga
     8641 tttatgatcg gatttttgat cgttattgcg gctattgtga tcgggatttt catttatgta
     8701 ctgacgatgc aaaagattaa tatctttggt gtaatgaaag cacaagggat cacaggagga
     8761 tttattgcgc gttctgtagt ggcacaaaca ttcattttat catttgttgg tattcttctt
     8821 ggtctattag gaacagtggg aacatccctt gttctacctg atgcggttcc attccaaagc
     8881 aattggctgt tttttggcgt cattagcttg ttgatgttag tagtagctgt gttaggcgca
     8941 ctattttctg ttcggacaat cgtaaaaatc gatccgttga aggcaattgg ataagaagga
     9001 ggaagaacat gaaagcaata gaatttaaag aagtagaaaa aagtttccaa gatggcgacc
     9061 aagtagttaa agcattgaaa gaaacaacca tgagtatcga taaaggggaa tttgttgcaa
     9121 tcatcggtcc ttctggttca ggtaaaagta cttttttgac tttggcaggc ggattgcaga
     9181 cgcctagcag tggctctgta tttatcaatg atcaagaatt cagtaatcaa aaagaaagcg
     9241 aacgcgttaa actacgtttt aaagaaattg gctttatttt gcaagcttcc aatctggttc
     9301 cttttctgac agtaaaaaag cagttattat tagtagataa gattaaacat gaacatcgtc
     9361 aaaaagaagc aaacgaatta tttgagcagc ttggcgtgga taagttgatc aacaagtatc
     9421 cagaagaact atctggtgga gaacggcaaa gggtcgcaat cgctcgtgca ttgtacaatg
     9481 atccgtccat tattctagca gatgaaccaa ctgctagttt ggattcagaa aaggcacgag
     9541 aagtagtcga tatcttggca aaagaatcaa aagacaaaaa taaagcaacg atcatggtta
     9601 ctcacgatac acggctgatt gacaaatgcg atagaatatt tatgatggaa gatggtgtat
     9661 tgaaagaaaa agaataacca aaaagcaggt ggaaaaatgg tccatcgata caacaaagat
     9721 acacagcaaa aaattttgaa agaagcgact gacttgttta tgtccaaggg atatctcggc
     9781 acttcaacaa gagaaatcgc acaaaaagtt gggatcaccc agcccaatct ctatcattat
     9841 ttcggtgata aagaaaaact atatacggca gttttagaaa accacttaaa agatgttgga
     9901 aaagctttga gagaaatcgt tcaaacaagc gaaacgggat ttcaagtaac cttgacaaaa
     9961 atggcgcagt ttttgattga gacacatctg gtcgatttgt ttctgatgct tcacgatctt
    10021 gaaagcaatc tttcaaagga aacaagagat catcttttct ttctatggaa gaaaaattat
    10081 cgtgaacctt ttgaagaaat cttttcagca aaccaatctg tgctaagaaa tgggatttca
    10141 aaagaaatcg cagcaaggca ttttttctta gtgttagcac cttacatcac tcggtcgaaa
    10201 agacctgtgg aagaaccgct aaatgtggcg caattgatcg atttatattt gaatggtgta
    10261 ttaggagaag cgtaaagctg ttatataggg agaaaatagt taggtgaaga agcaagatat
    10321 gacgaagatc gttatatctt gcttctttat ataggtagtt tttcataaat acaaatttgt
    10381 tattctctca tgattatcag ggtatatttt tatttattat gtcggtgttc caggaagtgt
    10441 tttaatttaa taacgaagaa attaattgtt tgtagaatga aggaaactat ttattgggta
    10501 gatgaatcac cacgaatagt caaaaggaga tttcaggata tgccaaaaat gaaaaaaatc
    10561 attttagcag taatgttatt ttctacggtt atatctttta cagccccttt tttgcttact
    10621 taagctgatt ttaagttttg gcccttagtc tggatctacg aggtgagagt caagaatgaa
    10681 aaaaataatt ggagcttttg tatttgcaat cattatcatt attatctgtt ttcaaataga
    10741 tgtttttggt caaaaaaaga tagttggaca aacggtcagc agtacgaaca gtacggttac
    10801 aaagacaaag gaatcatttg atgatacaga acaagagaca ttaatcaaaa tcggaaaaaa
    10861 tgaccgtaca ctatatacag atgactcgct aactacccca cttgcaacta taaacggcgg
    10921 agagctggcg gagtttcttt ctgaaacaaa aaatgcttat cagatcaaga caaatgatgg
    10981 ctatactgga tatttagccc tcgaagatgg aaccaaagtc acaaagaata tacaaacaaa
    11041 accggaagag ctatctgatg cagtgattgt attagatcct gggcatggcg gaaatgatac
    11101 tggggcatta agcaatgatg aacaaacaga agaaaaagat ataacattaa gtacagccat
    11161 aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt tatttgaccc atactacaga
    11221 tgaattagta cagcttggag atatatgcga ctacagtgaa gaaaaaaagg cagatgtctt
    11281 tatcagttta cacgcagact ctacggaata tgcgaatgaa gcaactggaa tcacgacata
    11341 ttattactat ggacaagagg aaacattagc acagacaatt gctgactcct tcactgatct
    11401 gcctctggat tcaagaggaa tctccactgg gaactatcaa gttcttcgag aaaatcttca
    11461 accttctatt ttaatcgaga tgggttatat gaataatgat agtgatttaa aggaattagt
    11521 aacagacagt tatcagcagc agattgctgc aagtttgaca caagcattga ccacttattt
    11581 tcagtaataa ataagcccag ccaaacaaaa atagctcctc atatttttcg tttggccggg
    11641 cttattatcg cagatgcaag cttttaaaca ccgtttaagg aagaggtcgt gagcctgccg
    11701 ttcaactctg agtcacaagg agcaatttca aaaaacagct tctcatgttt gatgactatt
    11761 gtgacttgtg accgaggagt tggcatgaga acaccgttta agaaaaaggt tgtgaaatca
    11821 acgttttgtt ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    11881 tgttgagcta ataccgagga tcagatgatt gaacaccgtt tattcggaac aagggactgt
    11941 gacatgactt ttgtcacagt tccattattt ttttagtagc ttcaaagctt tttctgtatt
    12001 ggcaaaatga agtttggcat atttttcaag cataggcatg ccgcctttct ccaatagttt
    12061 tgccgctact tggtcagatt tcgttccagc tcctgacgga agggtgattc cagcttcagc
    12121 tgatgctttg tctaactctt ccaaaaagct ggcacgactg atgatagaag cagcagcaac
    12181 agctaaatga tattgttctc ctttagtaac gaagtacagt ttttcttgta attgattttt
    12241 ttctaaacgc gcatattttt taaaatttgc ttccggcgta aactgatcga tcaaaatcgc
    12301 ttctggtttt tctggctcga ttttttttag cagcaaatgg atcgcttgat tgtgcaaagc
    12361 gactttcatc cgaacggcat tgtattttgg ttggatctca ttgtattttt ctggagataa
    12421 gacaagcagt ttatagggga tggttttttt taagatgtca gcaagctgga tgatttggct
    12481 atcttttaac tcttttgaat ctctaacacc aagtttacgt aattctgcaa gctgatcacg
    12541 tttcacataa gcagcacata ctgttagcgg accgaaatag ctgccatttc cgacctcatc
    12601 actacctaaa acggataatt gactgatatt ttttggcaaa gaagaggctg tttttgctgg
    12661 cttggcttgt ttatcctgtg cctcccccca tttatcagct tctattgctg cattcgtacc
    12721 ttgaaacatc acttttccgg atgtataagc ggtaatggtc gtcccgtctt tttttgcagc
    12781 aaagctagta taaggaatct gtttatttaa caaataagag cgatagtatg ctttcatttg
    12841 actaagttgg tcgtttgttg ctttgataat gacgttgctc ataaaattct cctcgttttt
    12901 atacataaaa ctataccgca actcacgact tttcgttttc taagagattg cgagacatag
    12961 cattttttta aagtttatca tataatagtt aagtatgtga aaaatatttg tggagaacgg
    13021 tgaaagaacc aacttcatgg tacactaaat aaaactttca tcaaatagga ggaaatgatt
    13081 cgtatggcgc acgaaaaaac acgctacaaa gcggtgattg ccaaccaaac atatactatt
    13141 atcggacggg aaaccaagca tcatatggat atcgtaacga agctgatcaa tgaacagtta
    13201 gcagaattga aacaattatc accacaaatg gataatgaac aagcagcaat cttgatggct
    13261 gtcaatgcgc tttcggatca attgaagaaa caagaaagaa tattggagct ggaagaagaa
    13321 acagcagaat taaagaaaaa aatgatcaaa tttacagagc ttgaaaatcg tgtgaaacgc
    13381 attgaagcaa tcgagaacga agctcgggaa gtattgaaag aaaatggtca agcagatcat
    13441 accattaaaa atcatgtgga agcacaacag attttaaacg agaaacgaaa aggacaaatt
    13501 aaacaaaaat cttctaatta agatcctttt atcaaaataa gaaaaattta aaaaagttag
    13561 aagagaaagg aacgtagcat gctgagtttg ctgattttat ttttattgct aattgcgttt
    13621 ttttctggag cacgtcgcgg gtttgctttg caaatcatat tcgcgattgg atatgtattg
    13681 tcatttattg ctgcccagca tttttacaaa ccattagcaa gtcatttgga attatacatt
    13741 ccatacccag ctgtcacccc aacatcaaag ttagcatttt ttgatcaggt atttgccttt
    13801 cacctagatg aagcttttta tgcaggaact gcttttctga ttattttgct aattggctgg
    13861 ctgcttaccc gatttgtggg ggtttttgtc catggattga cgtatgtgcc gatcttgaga
    13921 caggtagact ggattgcagg aggcatcctg tcactaatca tggcttacgt tactatattt
    13981 ttgattttgc agttacttgc ctttgttcca ttagatgtgg tccaaaatca attcggcgag
    14041 catagtttcg ctcgttttat cgttgagaga acgccatttc ttaccaacaa gatgtatgag
    14101 ctttgggtaa cgaatatttt gaattaagga aaatagtgct ggaaaatatt tcgttttttg
    14161 tacagagtag tttatttaag aaggctgcgt agcgtatgtc cggcacagct cttttttcat
    14221 agtgaaagat aaggtgataa aatgaataac cgaatccttg aaacattgga atttgaaaaa
    14281 gtcaaacaga tggtcagaca atttgtagtg actgcacaag gaaaagaaga actggcagaa
    14341 ttggtaccag tgagtgagaa acaaacgatc acaaactggc ttcaagaaac tgaggatgga
    14401 ttgaaagtgc aacgtctgcg aggcgggatc cctataccta aaattgaaaa tatccgtccc
    14461 catatgaaac ggattgagat cggagcagac ttgaatggag tggagcttgc acaagtttcc
    14521 agagtattgt ctacgacaag cgaattgaat cgtttcattg atgatctttc tgacagtgaa
    14581 atcgaatttg cacgtttgta tatgtgggca gatcagttag tcacgatccc tgttttgagc
    14641 cgccgtttaa aggaagcaat cgatgaagac ggacgcgtga ctgatgacgc ttctcctgag
    14701 ttaaagagca taagacagaa cattcggcga agtgaacaag ctgttcgtga acagttagat
    14761 gggatcgtcc gtgggaaaaa tgcaaagtat ttaagtgatg cgatcatcac catgcgaaat
    14821 gaccgctacg ttatcccagt caaacaagaa taccgcggtg tatttggcgg tgtagtccac
    14881 gatcaaagtg cttcaggtca gactttattt attgaaccaa aacaagtggt ggatttgaat
    14941 aatcgtttgc gtcaatacca aatcgctgaa cgaaatgaga ttcaacgtat tttaagtgaa
    15001 ctttctgctg aacttgttcc ccatcgtcaa gaaatcatcc acaatactta tgtgattggt
    15061 aaaatggatc tgatgaatgc aaaagcacga tttggtaaag aagtaaaagc catcgtgccg
    15121 ggaatcagtg aagacaatca tgtcgtcttg aaacaagcaa gacatccact gatcgatcag
    15181 gaaaaagtag tttctaatga catcacaatc ggaaaagact atcaagccat cgtcattaca
    15241 ggacctaaca caggtgggaa aacgatcacg ttgaagactt taggtctgtt acaactgatg
    15301 gggcaagcag gattaccgat tcctgcaggt gaagaaagtc aaatcggtat ttttgaggaa
    15361 gtatttgcgg atataggaga cgaacagtcg attgagcaaa gtctatcgac cttttcttct
    15421 catatgacga acactgtaga tattttatca aaagtcaatg aaaaaagtct ggtattattt
    15481 gacgagctcg gtgcaggaac agatccgcaa gaaggtgctg ctctagcaat cgcaatctta
    15541 gatgatcttg gtaaaaagtc tgcttatgtg atggcaacaa cgcattatcc tgaattgaaa
    15601 gtttatggat ataatcgggc aaatacgatt aatgccagca tggagtttga tgtggataca
    15661 ttaagtccaa cgtatcgttt gctgattggc gttcctggac gaagcaatgc gtttgaaatc
    15721 tctagccgat taggtcttga tacagaagtt attgatgaag cgaagcagtt gatgaatgat
    15781 gagagccaag atttaaacga aatgatcaca gatttagaga atcgccggaa aatggcagag
    15841 accgaatatc tggaaatgcg tcattttgtt tctgaggcac aagaacttca cgatgatttg
    15901 aaagaagctt acagctattt ctttgaagaa cgtgaaaaag aaatggaaaa agcaaagaaa
    15961 aaagcaaatg aagtcgtgtc agaggcggaa gaaaaagcag aaaaaatcat tgctgatatt
    16021 cgtaaaatgc aacagcagat tgggcaagga aatgtgaaag agcatcagtt gatcgatgca
    16081 aagacccaat tagcaaatct acatcaagaa gaaactttga agaaaaataa agtattgaag
    16141 aaggcgaaag aacaaaaaac gttgaaaccg ggagacgaag tgttggttac gacttacggc
    16201 caaagaggga cgcttttacg taaaaacggg aatcagtggc aagtagaaat cggtatatta
    16261 aaaatgaatg tgtccgaaga cgagctgacg cctgttgctc ctcaaaaaga gccgacacaa
    16321 cgagttatcc atgctgtacg atctgaatct agtagtcacg tgccgaatca attggacctt
    16381 cgaggaaaac gatacgaaga agcgctaagt gaggtagatc aatatttgga ttctgccatt
    16441 ctagctggtt atcctcaagt gactattgtc cacggaaaag gaacaggcgc acttcgcaag
    16501 ggaattactg attatcttaa aaaccatcgg agcgtcaaaa gctttgagtt tgcaccggca
    16561 aaccaaggcg gaaatggtgc gacaattgtt aaatttaagt aaagcttact aaaggtaagc
    16621 agatggttcg ctatccaagt atagaaatgc tatactagtc atagatttta aaaatggagg
    16681 tctttgatat gtcacaagta attacagata agacatttaa tgaagaaaca gataaaggac
    16741 ttgtattgat tgatttttgg gcaacttggt gtggtccatg tcgtatgcaa gcaccgattt
    16801 tggatcagtt ggaacaagaa tatgatgaag aagaattcag aattgctaaa atggatgtgg
    16861 atgaaaatcc agaaactcct caacaattcg gtatcatgag cattccaaca ttaatgttga
    16921 aaaaagatgg tcaagtagta gaaaaagctg ttggtgttca ttcaaaagaa caactacgtc
    16981 aaatgatcga tcaatattta taaaaactta cagcaaaaat aagctgaggg tgggactgaa
    17041 tccagttccg tcctcggttt ttttgcggtt gagttaaggt tattcaaatt aatatttttt
    17101 gagtataatt acgttgtcag gctggattgt ttttttgaca gacatgctat aataacttta
    17161 attgaatatg aaggggagta ccagatgaac gaacgaatca aaaacaaatt agctttactt
    17221 cctgatcagc ccggctgtta cttgatgaaa gataaaaatg gaacgatcat ttatgtcggc
    17281 aaagccaaaa tattaaaaaa tcgtgtccgc tcatatttta ctggtagcca taatacgaaa
    17341 acggaacggc ttgttagtga aatcgaagac tttgaataca tcgttacaga atcgaatatc
    17401 gaagcattgc tcttagagat caatctgata aagaaaaatg atcctaaata caacattatg
    17461 ttaaaggatg ataaaaccta tcctttcctt aagataacga atgaaaaata tccccgtttg
    17521 gtgattactc gtaaggtact aaaagacaaa gcactgtatt ttggaccata tcccgatgtc
    17581 ggtgcggcga atgagacaaa gaagatcctc gatcgattat ttcctctgag aaaatgcaaa
    17641 cctagtcaga cgaaggagcc ttgtctttac tatcatttgg gacaatgcct gtgtccttat
    17701 tatttcgacg tagatccgaa agtctatacg aaaatcgttg atgaagtgaa aaacttttta
    17761 aacggaggac acgaaaaaat cgagtcagag atccatcaga aaatggaaaa agctgctgaa
    17821 aacatggagt ttgaaaaagc tgcagaatac cgagaccaga tccgtgccat tgagacaatc
    17881 atgactcgcc aaaaaatgac aaatactgat ttactggatc gagatgtatt tggttatgcg
    17941 attgataaag gatggatgtg tgtccaagtg tttttcgtaa gacaaggaaa gctaattgaa
    18001 cgagatgttt caatgtttcc tttctataac gaagcagaag aagatttctt gacttacctc
    18061 ggtcagtttt atcaggaaaa tgagcatttc attcctaaag aagtcttgat tcctgatacg
    18121 atcgacaagc ccagtgtaga agctttgtta gctacgaaag tgctgcaacc aaaacgagga
    18181 gaaaagaaaa aattagttga attggcgagt aagaatgcct cagttgcttt gaatgaacgg
    18241 tttgatctga ttgccagaaa acaggaacgg acgatcggag cagttgaacg tttaggaaac
    18301 gcgatgaata tcccgacccc aatacgtatc gagtcttttg ataactctaa tatcatgggg
    18361 acgaatcctg tggctgcgat ggtcgtattc attgatggaa aaccggcaag aaaagaatat
    18421 cggaaatata aaataaaaac agtcaacgga ccggatgatt atgcatcaat gagagaagtc
    18481 atctatcgca gatattcgcg agtgattcgc gaagggttgc ctttacctga tttgatcttg
    18541 atcgatggag gaaaaggaca agtagatgtg gcaaaagatg tcttagaaaa tcaattagga
    18601 atcgatattc ctattgctgg catggtaaaa aacgataaac ataaaacaaa tgagttgcta
    18661 ttcggtacag atttggctgt agtaccgctt gagcgaaatt caccagagtt tttcttactc
    18721 cagcgaatcc aagatgaagt ccatcgattc gcaatcactt tccatcggca gctacgcagt
    18781 aaaaacagtt ttgcttcccg attagatgaa attgaagggt taggaccaaa acgaaaaaaa
    18841 atgctgctta aagagttcaa atcgttgaaa aatatcacag cagccacgac agaagaatta
    18901 caagaaatcg gactgccgaa aaatgtcgca caaaacgtat ttgataaatt acatcaggaa
    18961 tgaggaaagg gcactttaca aagaaagtaa agtgtcctat cattttatgt acaacaaaaa
    19021 gagcgtttac gttttgttgg taaataataa atcgcttacg tctggtgtct aaggatatat
    19081 gtgatttctt gaagaaggag tgaagaaatg ttagaagtca aggatcttgt taaaactttt
    19141 gggaactaca cagctgtcga tcatgtctct ttccaaatcc ccgatggaaa aatcatgggg
    19201 ctaattggcc aaaacggggc tgggaagaca acgacttttc gtttgatctt ggattttttg
    19261 acacaagacc aaggagaagt gttatggaat ggccatcatc ttggggagaa agattatgat
    19321 gtcatcggtt atcttccgga ggaacgggga ctttatccga aagtaacgat tcaagaccaa
    19381 ctgctttatt ttgcagaact tagagggaaa tcaagaaaag agattgaacc aaaaatcgac
    19441 ttttggatgg aaaaatttca ggtaaaaggg aaaaagacgg ataaagtaaa atctttgtct
    19501 aaaggaaatc agcaaaaagt ccagctgatc gccacgttaa tccatgaacc taaattgatc
    19561 attttggatg agccattcag cgggttagat ccggtaaatg ctgaattgct aaaagatgga
    19621 atattggaat tgaaaaaaca tggttcatgt gtcatttttt ctagccacaa tatggataat
    19681 gtagaaaaaa tctgcgatca tttgatcatg ttgcgaaacg gaaaaatggt tttgaatgga
    19741 aaagtacatg agattcgtga atcatttggc cgtacgaagt tattcttgga atctggttta
    19801 agccaacagg aagtagaaga aatcgaaggc gtgaaaaaag tgatccttca tgaagacaat
    19861 acgttagaga ttaccttaga agatcctgaa gctgggaaag aaatctttac tcgtgcgact
    19921 cagttcggct acattcctat gtttaaccag caaccgccaa ctttggaaga gattttcaaa
    19981 ttgaaagcag gtgaaatcga tgcgtaagtt ttggattatt accaaagatg tctatttaaa
    20041 aaatgttaag tcgatttctt tcttgatcat gatattagtc ccttttatca tgctgggtat
    20101 catttatgta gcaggaaact ttgctcaaca aaatagtgat gtcaataaaa tcggcgtatt
    20161 ggctgataat gagcaaatca gtcagcaaat gagccaagtc aaaacagatg attttgagtt
    20221 caaagcagtt gattctgaag aaaaagcaca aaaagagctt tcggaagaaa aaatcgatgc
    20281 gtatttagta gtctcaactg agaatgataa gataaatggg atgctttata gcgaaaattc
    20341 attaggacaa agtacacaac tactgataca acaacaactg actggcttgc aatcgatgct
    20401 tcgagcgagt caattaggca tttcaccaga agaagtagct agcttgagcc aacaagcaag
    20461 tttgactcga caaaaagtca attttgattc aaaaggaaaa atgacagtcg gagaagataa
    20521 ctcgacagtc caatacgtcg taagttatgt agcaacgatt gtcttgttca ttattatttt
    20581 gacgtatgcg cagatcattg cacaagagat cgcttctgaa aaaggcacgc ggatcatgga
    20641 agtgatcctg tccagtacga ctgcacaaaa gcatttttac ggaaaattaa caggtgtttt
    20701 acttgttgca ttaacgcaaa tggcattata cggtattatt tttggtatag gatacagtca
    20761 attcaaagat atggaaatgg tcaaaaatat attagacggt atctcattgg ataacttgat
    20821 tggaccattc ttgtggtatt ctcttctatt catgatcttt ggcattttga ttttttctgt
    20881 tttagcagct ttatgtggct ctttggtcaa caaagcagaa gatacagcaa aagcaatttt
    20941 gccagtgact tatctatctc taggcggata catgcttgga ttgatactag gagcttcaga
    21001 tccaaacaat atcgttattc ggatcacttc gtatattcca ttcttatctt catatattat
    21061 gccgattcgc ttagcaaatg agacagtcgg tatgggtgga gcattgattt ccttgttggt
    21121 acttgtagcc gtaacattct tattgatgat cttgtctgca aacatgtaca aatcgaatgt
    21181 actcgtttat agcgaaggcg gattatggag ttcattgaaa caatcgattt caattatgag
    21241 gaatgaacga aaaaaagcat aaaacaatcc taaaatacag aaggaagctg gacatctatt
    21301 gttcccagct tctttttgta tagacctatt ttatttattt tactatattg gatatatatt
    21361 cattgaaaat agtgtataac aactttataa tattgagtat accaattttt ggaggcgttt
    21421 taatggaccg ttatttagat ttactttcag aaaaattccc ctcaacggag gatgtcatca
    21481 cggaaatcat caatctcgaa gcaattttgc agcttccaaa aggaacagag ttatttttga
    21541 gtgatataca tggcgaattt cctgcttttg atcatattct gcgtatcggt tcaggaaatc
    21601 ttaaagaaaa gatcaaagac cttttttctg aacaaatgac ggaaacggat atcagccaat
    21661 tcactatttt tacggcttat ccagaatatg cattaacgac tgattggtac aaagaacaag
    21721 ataaatcgca gctgatccat catttgatcg atcttttacg gtttacgaca gtcaaatata
    21781 cccggtcaaa agttcgaaaa ggattgccga aagaatatag ctatatcatt gaggaactct
    21841 tgtatatcga tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc attgaacaac
    21901 ttgttttgat gaaggaagaa gaaaaatttt taacgaaact tgcacagacg atccaaaaat
    21961 ctgtcatcga tcatttgcat atcgttggag atatttttga ccgaggaact caagcagcca
    22021 aagtgatgga tcgaatcatg gatttttctt ctgtagatat ccaatgggga aatcatgata
    22081 ttttgtggat tggtgcgtat tgcggctctg aggcatgttt actgacactt ttacggattg
    22141 cagcacgcta taattattta tttgaattag agaaagaata tgggttgaat ttacgtccat
    22201 tgttttcttt tgctcatcaa acttatcaga agaatccagt atttgcgcct aaaaacagta
    22261 aaaatctatc cgaaagagag caagaagaat tagaaaaaat ccatcaggca ctttctatcc
    22321 ttcaattcaa atcagaacat cagttattgg atcgtcgacc tgaatttaaa atggacgatc
    22381 gtaaattgct ggaaaaaatc gactatgaag catcaacgat tgatttagaa ggtcagctct
    22441 atgagttgaa aggaacttgt tttcaaacga tccagccaga tgatccaaaa aaattcttgg
    22501 aagaggaaca aaaagtgatt gatagtttga tgtattcatt ccagcactct ttgagaatgc
    22561 aaaaacatat gacgtttttg atcgaaaaag ggggaatgta tctcacgaac aatcagcatg
    22621 tgctgtttca tggctgtata cctgtagacg agaagggaca gctcttagca tttgaattag
    22681 accaatctta tcgcggaaaa gaactgctgg gcttttttga aaaacagata actgaaagcg
    22741 caaaagatct gactgcaaaa gaagatttag caaccgattt gatttggtat gcgtggagtg
    22801 ggccattttc gcctttattc ggcaagagta aaatggcgac ttttgaacgt tattttctca
    22861 cagagcctca tacgcacgtc gagaaaagca atccttatta tcacttgcga gatgaagagt
    22921 ggttcagtga aaaaatttta gcagaattcg acacaaaaga agaagggtca gcaatcatta
    22981 atgggcacac gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag
    23041 tatttgtcat tgacggaggt ttatccaaag cttatcaaaa aacgacagga attgccggtt
    23101 attcactatt atgcaattct tatggtttcc aaatcgtgac acattatccg tttttgccta
    23161 ttgaacaaca atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc
    23221 aactaccacg caaactcaat cgtgatacaa caaaaggtat ggaactgcag cagcagatta
    23281 cccagctcaa aaggctgttg gactatcgaa aggacgattg agaagaattc cagtgaacat
    23341 tgttccatag actggagact gagacaaaag tcgtgtcgca gtctcttttt ccggataaac
    23401 ggtgttcttc tgccaactcc tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg
    23461 ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac ctcttggtat
    23521 aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa
    23581 gccattttca caaaattttt cttaatactc gaaactaaac agctttttca caaccccctc
    23641 agaaagtcta ttaaaattca ttcacaaaga aatcgaataa gcgctgttca gatggatgta
    23701 cacggtgggt aagttccgga tgccattgaa caccgaggat tttatgcata tcatctttgc
    23761 tttgaaccgc ttcaacgagt ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa
    23821 gatctttgac tgcctgatga tggtaagaat tcacaaaata tggatcgggg agaaggttgc
    23881 ttaacaaact gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg gcagtgggct
    23941 gttgttcatg ttttactgac cactttggat ataatgaaag atcttgataa agtgtacctt
    24001 ccaaaacgac attcaatagt tggagtcccc ggcagactgc aaaaattggt tttctttgtt
    24061 tcaatgcttc caagatcagt gcctgttcaa attggtctcg ttgaatattt gtttccgtta
    24121 gctttggatg aggatcttcc atgtaaagtt gtggactgac atcttgacca cctgcaagaa
    24181 taagtttgtc gatttgtcca atcagttgag gagctgaatc tggagacatg actggcagaa
    24241 taagaggcaa tccttgagca tctaaaactg catccacgaa tccttgcggt gtatagctca
    24301 cttgatttcc ttggaaagta tctgttgctc gaatcagttg atttccggca atgccgatga
    24361 ttggtttcat ttgtattccc cctaaagtag acttattgta acaaggattt aaaaaaaatt
    24421 aaactatgta agacttgccc ctatacttat ctgttcttgc tgtgttacta tctgtaaaga
    24481 gaaatttcgt gtgaaattca tgacgatagt tgaaaatatt gcttaatttg ttataataaa
    24541 aagtaagcag aaataaaaag gttgggcgta agcttcaagg atctagttct tcaaggggtg
    24601 agaaggacat tttattgaag gggcctgcct atactaagtt atctttagag gtacatactt
    24661 tcaatgaaaa aagaaagcat gtacctcttt tttaaatcga aaggaagtga agcaagtgtt
    24721 agaagctttg gaacaaatcc gaaaagcaga agaagaaaat gaaaagcgtg ctcaaacact
    24781 gcggcagcag ctgaagatct atgcaacaga aaaagagcag gaattggaaa cgatccaaac
    24841 aaactatcga caacaactaa aacagttggt ggaagaaaaa gaacaatttg aaatacaaga
    24901 attagaaaaa gaaaaaatga aattgaaagc agaagcagac aaggtgcaag caactttgaa
    24961 agatcaatat caaaagcatc agagtcaagc gatcggtgcg atcattgaaa gggtgaaaga
    25021 aacttatggc cgtcactaag atggaaaaag tgaccctcat ttcagataaa agaaatcagg
    25081 agaggatttt acaagcagta caaggaatcc aaaacgttga aatccgggat ctgtatcaaa
    25141 atagaacaaa caaccagtgg gtggagaagt attttccaga taccacgatt attgataaag
    25201 aagcaaaatt aagcgcttta ggtaatcggt tgaaagacat ccaagaagct atccaattca
    25261 tacaacacca cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa gaactttcct
    25321 tacaagattt ggaagccaat tattccgaag aaaatttcat aaataaattg aaagaagtcc
    25381 ttgatttaaa agaaaagtgg gaacaactaa ctgaagaacg tgatcgttta gaagaagagg
    25441 aagaatggct tcttcattgg caacagctgg atattgctcc aagtatgtat gcaagtaaga
    25501 cgaccatttt tgagatgggg actgtgagtg cagcaaattt tgaaagtttc aaggaagaac
    25561 tgagtaagct tgatgacgtt tatatggaag aaatagatta tacgccaaaa caagtttatc
    25621 tttcgtatgt tgctttaaaa aaatcagttg ctgttataga agaaattgct gggaaatacg
    25681 ggttcaataa agaaacgtat ccttatgaac aaaatcctaa agaaaaactg caagagacca
    25741 agcagcaatt atccattgtt cacgagcagc aaaagaagat aacgacagcg ttaggtcagt
    25801 gcagtggcta tatccaagaa tttgaatggg tagaagaagt cacacttgcc gttgcagaac
    25861 gagaaaaaat aaaagaacgg tttatccatg cgacttatct gatcgttttg caaggttggg
    25921 tagatgcaga agaaaaacag aatttgctgc atgtgttaaa cgaaaccgta tcagaagaag
    25981 atatatacgt cagtttcgaa acaccaaact cagaagagat tcagcaggaa gtccctacta
    26041 aattgaaaaa ccatccgctt gtcgaacctt ttgaattgct gactgaaatg tacagtctgc
    26101 caaaatatga agaagtagat ccaacacctt ggatgacacc attttatctt gtcttcttcg
    26161 ggatgatggt cgcagatgtt ggttatggtt tgctgatgct cattgggaca atactagctc
    26221 aaaaactact tgttctacca cgcgggatga aacgtttcgt aaaattcttt gagattctct
    26281 cgataccatc gatcatctgg ggattgatct acagttcatt tttcggacag tcattaccaa
    26341 aagaactgtt tggcatccgc ttgcctttcc caatcctatc tacgacggat gacgtcaata
    26401 cgattttgat tttatccgtg attttcggtc tgatacagat tttggttgga ttatttgtgg
    26461 cagcaaagga aaatatcagg agaaaagatt acttgagtgc tgtcagtgat ggatttgcct
    26521 ggcaagggat cctaattggg atcgttattg cgttggttgg tgccatgttg ataaaaaata
    26581 cagcatttgt ttaccttggc gctgggctat cgatcattgc tgccttatgt atcttgatcg
    26641 ttccgattat ccaaacgccg tctaaagtca aaggtgctgc caaaggtgcg tataatcttt
    26701 acggactgac cggctatatc ggtgatttag tcagttatac acgtttgatg gcattgggta
    26761 tttcaggcgg aagtattgga gcggcattca atatgctagt tgctttcatg ccgccagctg
    26821 ctcgattcag tgtagggatc cttttgatca tcgctttaca cgcactgaat atgtttttga
    26881 cactgctaag tgcctatgtc catggcgcac gattgcagta tgtcgaattt tttgggaagt
    26941 tttatacagg aggcggacgt gcctttgatc cgctgaaaac ggcggaaaaa tacgtcaaca
    27001 tcaatcacaa gaaaaaaaac aataaataat tggaggattt ttatataatg atggattact
    27061 taattagtca aaatggtgga atggtatttg cggtattagc gatggcaaca gcgacgattt
    27121 tttcagggat cggttcagct aaaggtgttg gaatgacagg tgaggcagca gcagcattga
    27181 caacaagtca acccgaaaaa ttcgggcagg ctctgattct gcaattgctt ccaggcacgc
    27241 aaggtttgta tggattcgtt attgcctttt tgatttttat caacttagga aatgacatgt
    27301 cagttgtaca aggactgaac tttctaggtg cttctctgcc gattgctttt acaggcttgt
    27361 tttcaggtat tgcccaagga aaagtcgcag cggcaggtat tcaaatttta gcgaaaaaac
    27421 ctgaacatgc tacaaaagga atcatttttg ctgcgatggt tgaaacatac gcgattttag
    27481 gtttcgttat ttccttccta ttagtgttga atgcctaagc ataaaggagg aaaagcagat
    27541 gaacgccatt gaaaaaatca tctctcagat gaacgaagca gcagagcagg aaagagcggc
    27601 gttagaacaa gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac gtacgcaagt
    27661 tgaaacagaa catcaaaaac aaaaagaaaa acaaatcgaa ttactcgaaa aaaaataccg
    27721 tcagctaagg aatcgccagc aagtagaagt acgacaagaa aatttgaatg cgaagcagga
    27781 atttttaaga cgattgtttg cagatgcggt aactgaaatg gaaaattggg atgaatctga
    27841 acaaatccag tttatcaaaa atgcattgta ttcattacct ttgacaggaa aagtagcatt
    27901 cattgctgga gaaaaatcag cagcctatct ttctcagacg ttgttggatg aatggaacaa
    27961 tgaacttccc tttatgatgg ttttaagtga tgaaactgtt gctgatcaag cgggttttct
    28021 gataaacgat caaggagttc agtataattt tcttttcagc agccttgtac aagatatcca
    28081 aggaacgatg agttttgaga ttgccaatca attattcgaa taaggaggga tcagtgtatg
    28141 gaataccacg aactaaatcc cttgattcgc ggaagagagc ttgaactttt gtccaatgag
    28201 acatttgaac ggatgattca aacaaattct gtcgaagcac ttggtgaaat attgaaatcc
    28261 acgatttaca gtccatatat ttatgacgga tatgaatatg acttcgaaca gaatctttta
    28321 aaggagcaga gccgtctatt tcgctggtta aaggaatcag caccggagcc tgaaatcgtt
    28381 tggatctata cgatgcgtta tactttccat aatctgaagg ttttgacaaa agctgagatg
    28441 acaaatcaaa atctggacca tttgtatatc gacgatggat tttattcatt agaaaccttg
    28501 aaggatgcca ttcatacgca agtttcaaat gaacttcctg aaagcattat ggacagtata
    28561 cgagaagtac gtgattattt tgaagagtct accattcttc agggaattga cgtgatttat
    28621 gatcgacagt ttttgacaga gcagcgacgg cttgccgata agctaggata tcaagaattg
    28681 ctgacagaag tcattgcttt tattgacttg accaatatca caacgatggc tcgaggaata
    28741 ttgcagaaaa gaacatatgg gttcatgacg acggtacttt ccagctcggg aagtatcgaa
    28801 aaagaagtat ttttgaactt tgtagataaa gatatggaag attacacgaa ttttctatta
    28861 gagacagatt atgctgattt tctgcgccct gtcatccgtg agggaaatat tgatctcgta
    28921 aagcttgaac agttgaaaga tgattatcta agttcattgt atcaagaagc acaaacccag
    28981 gctttcggac cactgccact actagccttt ttgaacgcaa aagaggtaga aagcaaaaac
    29041 cttcgtttac tggttatcgg caaaagaagc catttcgaat cagaaacaat cagaaagcgg
    29101 gtgagacaag tctatgacgc ataagatcgg tgtagttggc gacaaagatt ctgttttgcc
    29161 attcaaattg tttggctttg atgttcgtta tggtacaacc aagcaaaccg ttcgtcaggc
    29221 aattgaagaa atggcaaaga atgagtatgg cgtgatttat gtcacagagc aatgtgcagc
    29281 attgagccca gaaacgatcg aacgttacaa ggaacaaatg atacctgcta tcgtattgat
    29341 tcccaatcat caaggatcat tagggatagg gctggaagaa attcaaaaaa acgtagaaaa
    29401 agcagtaggt caaaatatct tatagtatga aaaggagata gaaattttgc aaattggaaa
    29461 aatcataaaa gtctccggac ctcttgtaat ggctgagaac atgtctgatg ccagtatcca
    29521 agacatgtgt ctagtgggag atctaggagt tatcggagaa attatcgaga tgcgtggcga
    29581 tgtggcgtct attcaagtat atgaagagac ttctggaatt ggtccaggcg aaccggttcg
    29641 ctctactggt gaagctttat ctgttgaatt aggaccaggg atcatttcac aaatgtttga
    29701 cggaatccaa cggccattag atacctttat ggaaatcaca caaagtaatt tcttagggcg
    29761 cggagttcaa ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac caacagtaga
    29821 agccggagaa acggtgagtg ctggagatgt tatcgggatc gtagaagaaa caaaagttat
    29881 aaaacataaa atcatggtgc caaatgggat aaaaggaacg atcaaaacaa tcgaaaaagg
    29941 ttcattcact attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg acaaaaattt
    30001 gacgatgctt caaaaatggc cagtaagacg tagtcggcca atcaaagaaa aattaaaccc
    30061 ggatgcaccc atgatcactg gacagcgagt gattgataca ttcttcccag tgacaaaagg
    30121 aggagcagca gctgtacctg gtccttttgg tgcagggaaa acagtcgttc agcatcaaat
    30181 tgcaaaatgg gccgatgtgg acatggttgt ctatgtggga tgtggcgagc gtggaaatga
    30241 gatgacggat gttttaaatg aattccctga attgattgac cctaatacag gagaatcgtt
    30301 gatggaacgt acggtattga ttgctaatac atcaaacatg ccagtagcag ctcgagaagc
    30361 ttctatctat acgggaatca caattgcgga atatttccgt gacatgggtt atgacgttgc
    30421 tatcatggca gactccactt cacgatgggc agaagcactt cgtgagatga gcggacgttt
    30481 ggaagaaatg cctggtgatg aaggttatcc agcttatctt ggttcacggc ttgcggaata
    30541 ctatgaacga tctggaagag tcattgcatt aggatcagaa ggtcgcgaag gaagtataac
    30601 cgcaatcagt gctgtatccc cgtctggagg ggatatttcg gaaccagtca cacaaaatac
    30661 attgcgggtc gtaaaagtat tctggggtct tgattccagt ttagcccaaa aaagacattt
    30721 tccttcgatc gattggatac aaagctattc tctttattca acagaagtag ggaagtatat
    30781 ggatcatatc cttcaagctg actgggcatc catggtcacc gagggcatgc gtattttgca
    30841 ggaagaacag cagctgaatg aaatcgtgcg tttggtaggg attgattccc tttcagataa
    30901 tgaccgtctg acattagaag tggcaaaatc cattcgtgaa gattatttgc agcaaaatgc
    30961 ttttgatgat gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt tgaagatgat
    31021 cttgaccttt ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt taagtgagat
    31081 catggacggt accgtatcga ttcgtgaaag gatcggacga agcaaatata tcccggaaac
    31141 agaattagaa aaactagatc aaattattgg tgaaatcaag caaacgatgc aaacgatcgt
    31201 ttcagaagga ggcatgagca atgattaaag aatatcgtac gatcggtgaa gtcgtcggtc
    31261 cattgatggc ggttgaaaaa gttgctggtg tcaaatatga agaattaatc gaagtccgta
    31321 tgcaaaatgg cgagattcgt cgtggacaag tattggaagt ccaagaagat aaagcaatgg
    31381 tccaaatatt cgaaggaaca agtggtatca atttacgtga ctcttctgta cgatttttag
    31441 gccatccttt agaactaggt gtatcggaag acatgatcgg tcgagtattc gatggtttgg
    31501 gacgaccgaa agataacgga ccagagatcc tgcctgaaaa gcatttggat attaacggag
    31561 aagtaatcaa tccagtagct cgggattatc cagatgagtt tatccaaaca ggtatctctg
    31621 caatcgatca tttgaataca ttagttcgtg ggcaaaaatt accagtattt tctggctctg
    31681 gtcttcctca taaagaatta gctgctcaga tcgcgcgaca agcaacggta ctcgattcat
    31741 cagatgattt cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa gctgaatttt
    31801 tcatggaaga ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt atgaatttag
    31861 caaacgaccc tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact gctgcagaat
    31921 atcttgctta tgaaaaggga atgcatgttt tggttatcat gacagacatg accaactatg
    31981 cagaggcatt gcgagagatt tctgcagcac ggcgagaagt tccaggaaga agaggatatc
    32041 ctgggtatct ttacacgaac ttagctactt tattcgaacg agcaggaaga atccgcggac
    32101 taaaaggatc ggttactcaa attccgattc taacaatgcc agaagacgat aaaactcatc
    32161 caatccctga cttgactggt tatattacag aaggacagat catcttgact agagagcttt
    32221 acaagagtgg gattcagcca ccaattgatg tactcccgtc tctttcgcgg ttaaaagata
    32281 agggaactgg acccggaaag acaagagaag accatgcagc tacgatgaat cagctttttg
    32341 cagcctatgc acaaggaaaa caggcaaaag aattagccgt agttttaggt gaatctgctt
    32401 tatctgatgt agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa gaatatgtca
    32461 atcaaggatt ctacacgaac cgttcgatta ctgaaacact tgatctaggg tgggaactgc
    32521 tatcgatgtt gccaagaacc gaattaaaac gaatcaaaga cgacatgctg gacaaatatc
    32581 tgccagaagg gaagtgagtt ctgatggcac gattgaatgt gaatccgacc cgcatggaat
    32641 tgactcgatt gaaaaaacag ttgacaactg ccacaagagg gcataaatta ttaaaagata
    32701 agcaagatga actgatgcgt caattcatcc tgttgattcg aaagaacaac cagctgcgtc
    32761 aagaaatgga agagtctatg acagaagcaa tgtctgattt tgtattggca aatgcttcga
    32821 tcacggaacc tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt gatttgtcta
    32881 tcgtagacaa aaatatcatg agtgtaaaag tacctgtgat gaattttgaa tatgatgaac
    32941 agctgacaga cacacctttg gaatacgggt atttgaattc cagtgccgaa ctggatcggt
    33001 caatcgatcg gtttactacc ctgcttccaa aacttttgga actgacagaa atcgaaaaaa
    33061 cttgtcaact gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat gcattagaat
    33121 atatgacgat ccctcaatta gaagaaacga tctattatat tcgtatgaag ctggaagaaa
    33181 acgagcgcgc agaagtaaca aggttgatca aagtaaaaaa tatgggtacc actgactaag
    33241 tggtatcttt ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa taaagaaacg
    33301 tttgccggct gttcagcttc tcgcattagg attttttctc ctgattttag ttggcggcgg
    33361 cctacttacc ctaccatttt ttagtaatag tgggcatggg acaaatttta tcgatgcgct
    33421 tttcactgca acttctgctg tctgtgtaac gggattgaca acattgaata cagcagagca
    33481 ttggaacagt gctggtcaat ttttgatcat gcttttgatt gagatcggcg gtcttggttt
    33541 tatgatggtt ccgattctat gctttgcatt gataaagaag aaagtcagtc tcagcacacg
    33601 tattatatta aaagaagcgc tgaatctaga agaagtatca ggtgtgatga agctcatgct
    33661 ttacatccta aaattggcct ttgtgatcca attgattggt acggttgctt tatcttttgt
    33721 atttgtgcct gaatttggct ggttgaaagg tatctggtac agtttgttcc atgcaatctc
    33781 tagtttttgt aatgcagggt ttgatttgtt aggagacagt ttagccaatc accagcaaaa
    33841 tgtttatttg atcatggtgg tatctgcttt gatcatttct ggtgggttag gcttcatcgt
    33901 atggcgtgat ttactggaat atcatcagaa aaagaagatg acgattcatt caaaaatcgc
    33961 attaaccgtt acaggtaccc ttttattagg cggattcgtt gtgttctttt tcacagagcg
    34021 caatggggca acgctagtgg aagggaatat gtttcaacga ttggcaaaca ccttttttat
    34081 gagtgtgaca cctagaacgg ctggatatta ttcagttgat tacctccata tgagccatgc
    34141 aggtctgatt ttaacgatgt ttttgatgta catcggcgga acatctgggt cgacggccgg
    34201 cggattgaaa acaactacgt taggtgtgtt gctgatccag atgcgttcga tgttgaaagg
    34261 acgtacgaga gcggaagcat ttggccgtac catccgacaa ggcgcagtgt tgcgggcact
    34321 tactttattt tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt tatcggtgac
    34381 tgaaacgata cctaaaactt ctggcatcga atatattgct tttgaggtct tctctgcgtt
    34441 tggtacagta ggtcttacga tgggattgac gccagacttg actgcaatag ggaaattgat
    34501 cattatagcg cttatgtaca ttggacgagt tggtgtattg actgtggtct tctctctgat
    34561 gacaaaagca caaagaacac aatcaaaata caaatatcca gaagaaagtg tcatgattgg
    34621 ataatttcat cttctgacag aaacctagtg atttagtaaa ataagaaaat aaaataattt
    34681 ttcttgcttc ttggatactt taagtgcaaa atgttcgttt attttttgag gcactacccg
    34741 ttgttgaacc tcgggtagtg cctctttttt attgaaagga atcaataaga tttgattcct
    34801 ttatttatca ataggttata attcatattt ttttattttt tcttttacga tacttagtcc
    34861 agaagctaaa atttcaaggt tattagagga tagggaaatt cgaacgaatg catctggttg
    34921 cgcacgtgta gtaaagcgtc tggaatgata aagtcggata ccgttagcaa gaaagtcatt
    34981 ctcaattatc ttagggtcaa tctcaggatc aactggaaga attcgatagt aagggacacg
    35041 attcgttggt ttttctactt tgaaaagaga atcaaaaagc tgattggctc tttttgtaag
    35101 acttaattta gtttctaaga tcctatttgc ttcatttgaa tggaggattt gagtgatgac
    35161 ttccacatct aatccagaag ttttcacatt tatattaaaa attgcttgtt ctatcttgct
    35221 ttggacatgt actggaacta caagataagc aactcgcaat ccagaacaaa taaattttgt
    35281 cattccagct aagtagattg tttggttagg taataaattt tggaaaggag gaatgatcgt
    35341 atgttgatag taagttgtta aaaatgaatg gatatcatct tcaataaccc atagaccttc
    35401 tttttcaatc acttcggcta attgctctcg tcttttgtga gagatttgaa accctatagg
    35461 attattacaa gaaggcataa gaaagacacc atgaatcttc ttttttctac attcctgaat
    35521 caatatatca ggtcgcattc cttgctcgtc aaaatcaatt ggaacgatct ctaaatgata
    35581 taactgagcc aattcaatga aattagcgta tgtatatcgg tctgttgcta ttctatcacc
    35641 tggagagaaa agtgcagaaa gtgtgatagc caatccattt tgaacaccgg aaacaataga
    35701 tagagattct aaggacggaa tgactccttg agtttttaac caatttgctg cagtagtcaa
    35761 ttgaaaattt gtgcctttgg gatcttggta attaagcaaa ccaacaagat ttgggttatt
    35821 actagttgat tgaataaacg ggacaacgaa tttattacat tcttcaaaag aactaaccag
    35881 accaaaatca atgatatgtt cggttacttc attggtagag atggtgatgg actcaaagac
    35941 attgggagaa ataaatgtgc cacttccaat attcgtatac agcaaacctt tctcaattcc
    36001 atatttataa gccttgccaa cggtagtaaa atttaaatct aaataatcag ctagctctct
    36061 ttgggagggt aactgagtgt ttttctgtaa tttattcttt aaaatatctt gttctaattg
    36121 ttgaatcaac gatcgataat aaggacgctt caatttttgt ttgtctggtc gccaagttaa
    36181 cgggtaattg tcataagaat taataggcat tttctccact ccttaaaatt gtataccata
    36241 caattttacc attgattgta ttgattttag aagttaatct tttgtgagga gggaattgtc
    36301 tatgaaaatt attaattttg aggaagtagt agcttctttt agttttgaag aagcgattca
    36361 agtaatgaaa aaatgttttt ttgattatga aagtggtaaa atcagccaaa atcctcgatt
    36421 ggtggaaatt ttacctgacg gaaaaggcaa aaatatgttt gcgatgatgc ctgcttattt
    36481 gggggagaac agatattttg gcacaaaagt cattaccgct tttccggata atcatcagta
    36541 tcatttacct tctcatttag gagagatttt cttgtttgat gctcaaaacg gactacccaa
    36601 agcattgata aacgcaaatg cagtcacctg gattcgaaca gctgctgtat ctgcgctggc
    36661 aacagattac ttagcaaatc cgcatgcaga aacacttgct ttaattggag ctggtcaaca
    36721 agcattgtcg catttgactg cgatttctac cattcgtccc atcactacag tgtttgttta
    36781 cgatatttta gaagaaagac gaaatacttt tatcgctaaa gctaaaaaac agtatcctaa
    36841 aataacgttt ataaatgcaa tatcagtaga agatgcagta cgtccagcgg aaatcatttg
    36901 tacgttgacc tctagtaaag aggcatttct tcaaaaagag tgggtaaggg aagacgcaca
    36961 tatcaatgct gtgggcacat ttactcctga tacaagagag ataagtacat cgttgatgga
    37021 aacttctcaa atctatgtag atgattactc agctgcctta acagaaagcg gggacctgct
    37081 tatagccatg gcagaaggag atttagagga atcttgtatt gttggtacat taggcgaatt
    37141 agtttctgag aaaaaaacat tttataaagg agaatcgagg ccgacgattt tcgacgcagt
    37201 tggtctagct attgaagatc tttgttgtgc agaatatatt tataacaaat tagagaaagg
    37261 ggctttaaaa tgaacttaga ttattacgta gttgatgcat ttgcagatga ggtgttcaaa
    37321 ggaaatccag cagcggttta tgtgctagaa gaatggctgc cagaaggtac gatgcaaaaa
    37381 attgccattg aaaacaattt atctgaaaca gcatttacag tgaagaaaaa ccaagaattt
    37441 gagcttagat ggtttacgcc agatcgagaa atcgatcttt gtggtcacgc gacattagca
    37501 acagcatttg ttttatttaa ctattacaaa atacctgatg aaacgattaa attttctact
    37561 caaagcggta atctatttgt gacaagaatt aaagattatt attacatgga ttttcctagt
    37621 attatgccga aaaaagtgcc gatattgtct gaatatgaag aagcgattgg ggctaagatt
    37681 aaagaagcct acttagcacg tgatttattt ttcgtattag aagatgaaaa aacggtggct
    37741 aaattgcagc cagattttac agctatcaaa aattttgaat taggaatagg agtcatcgtg
    37801 acagcagaaa gtgtgcaaaa agactttgtt tctagaacct tctttccaaa attaacaatc
    37861 aatgaagatc cagtatgtgg atcagcacat tctaatctta ttccttattg ggcagaaaga
    37921 atgaataaaa caaaattgtc agcttatcaa atgtcatcaa ggggcgggga tctagaatgt
    37981 gaattaaaag aagatcgtgt gataatagga ggaaaagcag ttttgttttc taaaggtacc
    38041 tcgtttattt gatttctttc aaatgaatta aaaataaatt ggtggagagt ggcaacctta
    38101 tagctacaat ggttgttaaa aaaagacgca aactttttca atttaatgaa aagtttgcgt
    38161 ctcattttaa ttattggtgt ttgtttttta ttcaatcaag agtaaaatgc cccatactaa
    38221 ctatcctcgg atgacttcga ttttataacc atcagggtca atgacaaagt agtaagaagg
    38281 cgcagtgcca ggtaatcctt ttaagtcagt aatctcaaga ccagcagctt tatgttgctc
    38341 gtgtaatttt tctagatcat cagctgctat agcgatatga ccatagccgt ctcctagatc
    38401 atacgcacca tgatcatagt tgtaagtcaa ctctaattca tagtcatccc caggaagtgt
    38461 caaataaaca agtgtaaatt tgttttctgg aaaatcacgg cggcggcttt cttcaaaacc
    38521 aaatgccttt tgataaaatt cgattgatgc ttcaagatct ttcactcgaa cacatgtgtg
    38581 agccattttc atagattcat ctctcctttt ccttttactc aatcataaca aaaagaagta
    38641 aggaaatagt tgatttgtgc ttgttcatga gaaatttctc atgtaaaatg gatagaaaat
    38701 aaaactgtta aggtggaaac tattcatgga taaaccaatt tttggtgaaa aaaagcaatt
    38761 tgctgattat caaacccgtt atgcagcata tattgtcata gaaagagatc agcagattgc
    38821 agtaatcgag gctccaaatg gtgctttttt tcttcctggc ggggaaattg aaggagaaga
    38881 gacgaaagaa gaagcgattg tccgtgagct gatagaagaa atgggaattg tggcagaaat
    38941 ttcttcttat ctgggagaag ctgatgagta tttttactcg aattatcgtg caacttatta
    39001 ttataatcca ggatatttct ttgttgctga ttcttggaag cgtataggag aaccaactga
    39061 aaagaccaat aaaatttggt gggtgagccc tcaagaagcg cttgtgaaat tgaaacgtgg
    39121 tagccaccga tgggcagtcg agagatggat cgaaaaaaat aaaaactgaa agaaagcgag
    39181 tagatcagct ttctctcagt ttttttattt attacagtac atattttttg attgcttcag
    39241 ctacaccgtg ctcgtcgttt gatgaagtga tgacatttgc tgcttctttg acaagagaaa
    39301 cggcatttcc cattgctact cctagaccag catattcaat catggataag tcgttctcgt
    39361 tatctccgat agccatgatt tcatcttgat caataccaag gtgctgtgcg agattcgcaa
    39421 ctgccgcacc tttacttgct tctttattta gtacttcgaa gtaatagggc gcacttttga
    39481 ccgttgtgta tttatcacgg aaagtttgcg gtaaacgcgc aattgctgca tctaaaattt
    39541 ctggttcgtc gatcatcatc attttgacga tgttcatgtc tggtgtcatt tcttcaggtg
    39601 ttcgatattt tagcggcatc ttgactaaac ttgcttcgtg gatcgtataa gcgctgatat
    39661 ctcgattagg cgtgtagatc gctttgtcat cgatcgaatg gagatgactg ccgacacggc
    39721 gtgccattac ttcgatctct aggaaatctt catgtgtcag tccgtgacgg gcgatgattt
    39781 tacctgattt cgtttgttgt acgagtgccc cgttataagt aataacgtaa tcattatctt
    39841 gaaaaaggtt caactcgttt aactgttcct ggacacctgg taagggacgg ccagtgcata
    39901 aaacgatcga aacacctttt gcaacagcag cggcaatcgc ttcttttact tcttttgtta
    39961 cttctctttt ctcatttaat aaagtaccat cgatgtcgat agcgactagt ttgatagaca
    40021 taatttcctc caatatattt taatttattt caatcaatgc gccattacgg atatggctgg
    40081 caaattcttg ataagtttcg tcaaataaat cataatgatc acgcaaggac ggatcaacca
    40141 tttcttttgg aaagaaaaat cgctcatctc cttgttcttg tcccgctaat gcagcaacaa
    40201 gctgactaac ctgtgataat tctatcaaag ttccgtcatt tcgcaaaatc tcgatttgtg
    40261 tgcgatgtct gccttgttct gggcgataaa aatcataagg tagatcgtag ctagaattca
    40321 cagcagtgta ataaacagga ttatatccga ccttttctac taacaaagtg agttcttgga
    40381 ctaattttga atcccggttg ccagaaaaag tagcagattt cagtggttta cgattcaaaa
    40441 aacgtttagc taaatcattt aaaataggat cagcagattc agaccattga gtaaagtaag
    40501 tgcccaagac cccatcatca agtttgaggt attcttctaa agtaaagctt ccgtcaagaa
    40561 atggcagaag taattgagaa tggagttcaa aactgctggc atcttcttga tacaactcgt
    40621 gtgcgcgatg aagcagatgc tccaaaatta cttccatacc acgggaaaca ggatggaagt
    40681 acacttgcac atacatttga tatcggctaa cgatataatc ttccactgca tgcatgccac
    40741 tcatggaaaa agcgatcccg ccttcataag gacgaatcac acgtaatatt cgggtaagat
    40801 caaatgttcc atattctgtt ccagtgaaat aagcatcccg aagcaaatag tccattcgat
    40861 ctgcatcgat ctgactagaa atcatttgga caacttgtgg gttggggtaa gttttttgga
    40921 tcacactagc taccttttca ggaaaacctt cttctactcg gttaagaatc tgatacactt
    40981 ctgtttctgg agaagtgatg atctctacag tgatggcttc atgattcgta tggaagatat
    41041 gttcaaatgt atgagagtaa ggaccatgac cgacatcatg caacagtgcc gcacataaag
    41101 tcaccagccg ttcctgatca tcccagcctc cgttgcctat tttttctttt gaaaaatttc
    41161 gagaaaagat atcgcagatt cgtcttgaaa tctcataaac gcctaatgaa tgagtaaaac
    41221 ggctatgttc tgctccgtga aaagtgaaag aagaagtgcc taattgttta atgcgtcgta
    41281 gacgttggac ttctttcgaa ttgatcaaat ctaagattac ttgatgctgg acatggatat
    41341 agttatggac tggatcacga aaaacttttt ccataggcag catttgatat ttataagggg
    41401 ttgtcataat gtagctcctt tatgttatta cgttgttcca ttctctggat ttggttgttc
    41461 cattgctcca gctggatttc tttttttagc cagtcggtag aatcctgtaa atcacttgca
    41521 gaatacagct tttcaaaagc ttttgcaaat gcttctttga cttcttttac aaaaaggggt
    41581 tggtcaagca gttcttcaag tgttgccatt gtagcaggat ccactgcagg atagccattt
    41641 gtaccaaagt cttcttttaa agcagattga taaaatcgct tcatgacttg cccacgtttt
    41701 tcctgattac cgttgacact taagtacatc ataacagaaa tcccattttt gatccgacgc
    41761 tgagcgatac cggcaaattt tttgccatgg atgcttaaat cataagttcc aggacaataa
    41821 gaatgggtga tttcgaatgc ttgtatcggt gcctttggaa aagcgtattt agtcaatagg
    41881 agcatttgct cgtaagcgtc atctatggtt attttttttg tgtcactttg ggggaaaatc
    41941 aaagaaatat tcaaaattcc ttcatctgat acgatcccta atccacctga atttctgatc
    42001 actggagcgt aacctgcatc ttccagtgtt tggatcccgt cacttagaaa aggagttcga
    42061 acatccttca tacctaagat gactgtgcag tcatattgcc agaaatgtaa aaagatttgc
    42121 tggtgttctt tagaatattc cgttaatata tctgttaatg caaaagggga aaagtcgttt
    42181 ttttgataaa tgtgctgatc tagtcgaatc gcagggttca ttcaaccggc tcctttgtat
    42241 aaattcatat tttctacaat attatactct aaaatcaaag agttgttttg gaaaaaaggt
    42301 tttctgtaaa aatggacaag ttggttgaca aaacaaaaga aactaggaac aatgtagcta
    42361 tgagaagtaa aggagaatga ataatggaat tatcacaagg aacaccgatt tcaatcaaat
    42421 tgcgtacaaa agtaaaacag caaggggaag tgcaagattt ttattttgat ttgaaagggc
    42481 aaatggtgac aattggcgat acgctgtaca tacgttataa agagattcag gaggagacgg
    42541 gcgatgagat tcctgtaacg atcaaattag ttccagatgg tcatgtacag ctgatccgtg
    42601 caggagaaat gcggatgcgt ttgaaatttg gctataaaga acggttagaa acatcctatc
    42661 gaacaccata cggtatgatc cagattgcta cttttacgaa agaactgcat gttagtttga
    42721 aagaccgtcc cacagcaggg aaggttcgaa tcgattatga tctgttcatg ggaccggaaa
    42781 aaatcggcga atattatttg accttggatt ttacagcgta aatataaaag aaaagtttga
    42841 ttttttgtgt ctgattttgt atgataagga gtagactgtg aaaggacgtg tcctgattgg
    42901 aaattagtgt atttgaaggt gcaaacaaaa gcgaattatc aatgatcgaa gtagcacacg
    42961 cgatcttaga acaacgtggc gatgtcatgg atttttctga tttagctaac cagatccaaa
    43021 actatcttgg gaaatcagac agtgaaattc gtgattcact cgcacaattt tatactgact
    43081 tgaatattga cggcagcttt atctcattag gggataatcg ttggggcttg cgttcatggt
    43141 atgctatcga ttcgatcgat gaagaagtaa accatggaat ggacgaagag gatgaagata
    43201 ctccacgtcg ccgtaaacgc aagaaagtca acgcctttat caatgatgat gaagacgcga
    43261 tcgattacaa tgacgatgac ccagaagata cagatctgac agaagaagat gacgatgatc
    43321 tgtttgatga cgatgatgat gaagatgaag aaatcgctgc ttataattct gacttgcaag
    43381 aaatcggagc agatgacccg acagatgatg aagaagactt gccaggaaat atcgaagaag
    43441 atttaagtat catagacgaa gacgatgatg atgaagatta tgaagaggaa gaattttctg
    43501 actctgaaaa agaataaaca ttattttctt acgagaggtt gtgacagaga cgatcagcct
    43561 atcagaataa ggaggtgtga cacg
//
LOCUS       DACECY010000021        42187 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.022, whole genome shotgun sequence.
ACCESSION   DACECY010000021 DACECY010000000
VERSION     DACECY010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.022"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            155..1018
                     /locus_tag="IWO38_001162"
     CDS             155..1018
                     /locus_tag="IWO38_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0313262.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1147..2769)
                     /locus_tag="IWO38_001163"
     CDS             complement(1147..2769)
                     /locus_tag="IWO38_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0313263.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3018..3539
                     /locus_tag="IWO38_001164"
     CDS             3018..3539
                     /locus_tag="IWO38_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313264.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3725..5455)
                     /gene="spxB"
                     /locus_tag="IWO38_001165"
     CDS             complement(3725..5455)
                     /gene="spxB"
                     /locus_tag="IWO38_001165"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ0313265.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5620..6438
                     /locus_tag="IWO38_001166"
     CDS             5620..6438
                     /locus_tag="IWO38_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ0313266.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6541..6738)
                     /locus_tag="IWO38_001167"
     CDS             complement(6541..6738)
                     /locus_tag="IWO38_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313267.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6907..7485
                     /locus_tag="IWO38_001168"
     CDS             6907..7485
                     /locus_tag="IWO38_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ0313268.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8530..9033)
                     /locus_tag="IWO38_001169"
     CDS             complement(8530..9033)
                     /locus_tag="IWO38_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0313269.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9392..10417
                     /locus_tag="IWO38_001170"
     CDS             9392..10417
                     /locus_tag="IWO38_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0313270.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10740..11618
                     /locus_tag="IWO38_001171"
     CDS             10740..11618
                     /locus_tag="IWO38_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313271.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12094..12210
                     /locus_tag="IWO38_001172"
     CDS             12094..12210
                     /locus_tag="IWO38_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ0313272.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13138..13476
                     /locus_tag="IWO38_001173"
     CDS             13138..13476
                     /locus_tag="IWO38_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313273.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13570..13800
                     /locus_tag="IWO38_001174"
     CDS             13570..13800
                     /locus_tag="IWO38_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313274.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14200..14571
                     /locus_tag="IWO38_001175"
     CDS             14200..14571
                     /locus_tag="IWO38_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ0313275.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14845..15537)
                     /locus_tag="IWO38_001176"
     CDS             complement(14845..15537)
                     /locus_tag="IWO38_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0313276.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15540..16325)
                     /locus_tag="IWO38_001177"
     CDS             complement(15540..16325)
                     /locus_tag="IWO38_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0313277.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16562..17962
                     /gene="lacG"
                     /locus_tag="IWO38_001178"
     CDS             16562..17962
                     /gene="lacG"
                     /locus_tag="IWO38_001178"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ0313278.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            17987..18304
                     /locus_tag="IWO38_001179"
     CDS             17987..18304
                     /locus_tag="IWO38_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0313279.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18317..19990
                     /locus_tag="IWO38_001180"
     CDS             18317..19990
                     /locus_tag="IWO38_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0313280.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20004..21635
                     /locus_tag="IWO38_001181"
     CDS             20004..21635
                     /locus_tag="IWO38_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ0313281.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            22507..23697
                     /locus_tag="IWO38_001182"
     CDS             22507..23697
                     /locus_tag="IWO38_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAQ0313282.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            23831..26020
                     /locus_tag="IWO38_001183"
     CDS             23831..26020
                     /locus_tag="IWO38_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0313283.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            26017..27810
                     /locus_tag="IWO38_001184"
     CDS             26017..27810
                     /locus_tag="IWO38_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0313284.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            28010..28324
                     /locus_tag="IWO38_001185"
     CDS             28010..28324
                     /locus_tag="IWO38_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0313285.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            28345..28722
                     /locus_tag="IWO38_001186"
     CDS             28345..28722
                     /locus_tag="IWO38_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0313286.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            28732..29505
                     /locus_tag="IWO38_001187"
     CDS             28732..29505
                     /locus_tag="IWO38_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313287.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            29527..30930
                     /locus_tag="IWO38_001188"
     CDS             29527..30930
                     /locus_tag="IWO38_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0313288.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            31112..32296
                     /locus_tag="IWO38_001189"
     CDS             31112..32296
                     /locus_tag="IWO38_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0313289.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            32293..32583
                     /locus_tag="IWO38_001190"
     CDS             32293..32583
                     /locus_tag="IWO38_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313290.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            32580..32801
                     /locus_tag="IWO38_001191"
     CDS             32580..32801
                     /locus_tag="IWO38_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313291.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            32843..33622
                     /locus_tag="IWO38_001192"
     CDS             32843..33622
                     /locus_tag="IWO38_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAQ0313292.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            33689..34183
                     /locus_tag="IWO38_001193"
     CDS             33689..34183
                     /locus_tag="IWO38_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0313293.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            34239..34877
                     /locus_tag="IWO38_001194"
     CDS             34239..34877
                     /locus_tag="IWO38_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313294.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            34949..35344
                     /locus_tag="IWO38_001195"
     CDS             34949..35344
                     /locus_tag="IWO38_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0313295.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            35328..37781
                     /locus_tag="IWO38_001196"
     CDS             35328..37781
                     /locus_tag="IWO38_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0313296.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            37778..39805
                     /locus_tag="IWO38_001197"
     CDS             37778..39805
                     /locus_tag="IWO38_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313297.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            39802..40824
                     /locus_tag="IWO38_001198"
     CDS             39802..40824
                     /locus_tag="IWO38_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0313298.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            40841..41770
                     /locus_tag="IWO38_001199"
     CDS             40841..41770
                     /locus_tag="IWO38_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0313299.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            42015..42131
                     /locus_tag="IWO38_001200"
     CDS             42015..42131
                     /locus_tag="IWO38_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ0313300.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT        13813 a         6975 c         8340 g        13059 t
ORIGIN      
        1 aattccttct tatttcttga agctgaccac ttctgtcaca acctcttatt ttgattagaa
       61 cttgcgaaaa taaaccggtt aaagtgtgtc tagcgcaaga gatttgctat aatagacgat
      121 aagggtaaaa tgtaacgaga aggggttgaa aaatgtgtat caaattgtaa cagattcttg
      181 ttgtgatctg ccataccatg tattagcaaa agaaaaagtt gattttttat caatgaatct
      241 ccaattgaat gatcaagagt tagtagatga cctcggaaag acattcaatt atgaccgcta
      301 catgaatcag ctgaaaaatg gtgcgatgcc tacgacatca caaatcaatg ttggccgata
      361 tttagaattt ttccgttctt atgcggaaaa acagattcct gttttatatg tggctttttc
      421 cagtggatta agcggctctt acagtagtgc gcttcaagcg gttgaattgc tgaaggaaga
      481 atatgaagac cctgagatct atgtttttga tacaaaagct gcaagtattg gggaagggct
      541 tttagtattg gaagctgtaa gattgaaaaa agaaggtaaa agcatcgaag aaaccctaga
      601 gtggctggaa agtaattatt tgaaagtcca ttcatgggta acagtagacg atctgaaata
      661 tctagaacga ggcggtagga tttccaaagc aagtgctact cttggcagtc tattgagtgt
      721 aaagcctatt attacggtgg ataaagaagg aaggctgacg aatgttgaca aagttcgagg
      781 aagaaacaag tcgatccaga caatcgtcaa tgagacggta aaaacaatcg ttgcaccact
      841 tgatcaaatc gtttatgtgg cttacgctgg ggatctagag agcgcggaga aggcaaaacg
      901 gcttttggaa gagcagataa agatgaaaga tgtgaagctt tatcccttag gaccaacgat
      961 cgccagtcac acaggctatg gctgtatcgc gattttttcg atgggaatca gtcgataaaa
     1021 gcagtaactc attctgtaaa aaagcccaga aattgattgt aacaaaaaca gcgactctta
     1081 aaagatagat gagcatccta tctcttagag gtcgctgttt ttttattttt taagctaaat
     1141 aggccattat gcccataact cgttcaattg tttttgaata tctgcatttt ccaaaaattc
     1201 atcatatgtc gtatcagctc gatcgatcac acctttatcc gaaacggcaa tgatgcggtt
     1261 tgccaatgtt tggataaatt gatggtcatg tgacgcaaat aagattgagc cagtaaatgc
     1321 catcaagccg tcattcaatg ctgtgattga ttctaagtct aaatggttcg ttggatcatc
     1381 taatacaagt acgttagctt tcgaaagcat catttttgaa agcatgcaac ggactttctc
     1441 tccaccagat aaaacattca ctggtttcaa tacttcttca ccagaaaaca acattcttcc
     1501 taagaagctt cttaagaagg tgttatcatc ttcttcttta cttgcatatt gacgcaacca
     1561 gtctaaaatc gttagatcag tatcgaattc tttcgttgta tcctttggta aataagaatt
     1621 acttgttgtc acaccccaac gtactgttcc agtatctggc gtaatttcac ccatgataac
     1681 tttgaatagc gttgttgttg ttatatcatt ttctgcgacg aaagcgacct tgtcttcttt
     1741 gctcaaatta aaagatatat catctaaaat tttctttcca tcaatcgtta cacttacatt
     1801 ttctacctgc aataagtcgt ttccaatctc acgttcaggt ttgaatccaa cgaatggata
     1861 acgtctagaa gaaggttgga tatcgtctaa agtaattttt tcaagcattt ttttacgtga
     1921 agttgcttgt tttgattttg atgcattcgc actaaagcgg gcaataaatt cttggagttc
     1981 tttgatttgt tcttcttttt tggcatttgc gtttgcttga agtttagccg ctagttgact
     2041 agattccaac caaaaatcat aatttcctac ataaagtttg attttaccaa aatctagatc
     2101 cgccatatgt gtacatactt tgttcaaaaa gtgacggtca tgggatacaa cgatcacagt
     2161 attttcaaaa ttgatcaaaa attcttccag ccaattgatc gattggatat ccagaccgtt
     2221 tgttggttcg tctagcaaaa gaacatcagg tttaccaaat agtgtttgtg ctaataacac
     2281 ttttaccttt tgtccaccag ttaattcact catttgctga tggtgcaatt cttcagggat
     2341 gttcagacct tgcagtaaaa cagctgcttc tggttcagct tcccatccat taagctcagc
     2401 aaattctcct tctaattcag ctgctcgaat accatcttcg tcagtgaaat cctctttcat
     2461 gtaaatcgca tctttttctt tcagcacttc atacagacgc ttgtgtccca tgatgactgt
     2521 ttcaagtaca gtatagtctt catagtcgta atggttctgt ttcaatacgg ccatccgttc
     2581 atctggtccc atagtaacgg aacctgtgga aggctgcaga tcccctgata acactttaag
     2641 gaacgttgat ttacctgcac catttgcacc aatcaagcca tagcagtttc cgggggtaaa
     2701 tttaatattc acatcatcaa atagtttgcg gtctgaaaag tgcaaactca catcatttac
     2761 agtaatcaat ttttttcctc acttttatca ttattagttt tgacaagtct tatagaatta
     2821 tataggggaa aggtaaaaga ttcaaggcaa tcgaacattc tttgtgtttc acgtgaaaca
     2881 gaatgaaaaa aataaaaata aaagcggagc gaaaatagct actataggat ttattagttt
     2941 gcaacagcat aatttttgac gttatttata agagggagta ctataaaact gtaaaagaaa
     3001 tgaaagaagg attgaaaatg gatgaaaaaa gaaccatcga ctggggctca ttgattttgg
     3061 gaattttatt tgtacttgtc tcattgttat catttcaaga ccctgtcgga aatttagttg
     3121 caattgtagt agtctttgcg atttttgcct ttattaaagg gatatttgaa ctgtttgtac
     3181 ggaacagatt aaaagagcta acaggatata aaggaaagat gcccttaata atcgggatca
     3241 tcgatattct agttggcgtc tttttcttat tcaatattgg tgcaggagtc gcagcattgc
     3301 cttttgtttt tgcagtatgg tttattgctg attcagtttt agcgttattt actgctgact
     3361 tggcaagagg agtaagtgaa ggatattatt ggtttacgat catcgtcaat attttaggaa
     3421 ttttattagg aattttctta ttgtttaatc caatttcttc tgcgttgaca ttaagctttt
     3481 tagttggatt ctattttatg ttatttggta tcacacatat cgtctatgca tttagataag
     3541 taaaatcaaa caaataaaaa agccggcttc cgattacagt tttactgttg taattggaag
     3601 ccggcttttt tatcatggac aaatatgcac gaggtgattt ttaggtgtcg agacaaattt
     3661 atgcaaaaat gtgagaagct atatacacaa attttatctt aatactcgaa gcatagcgca
     3721 gttgtcacaa cctcttttca tataattcat tcaatactgg caaatcatgg acttcatatt
     3781 tttctttgaa tgcttggatt tcttcttcag aatattttct tggatctaaa tgcagatctt
     3841 ctactggcaa tgggcgcttg ttattgatct tcacatcgat tactacagga cgatctgatt
     3901 ttgcagctgc atcaaaggca aatcgaagtt gcgagtattc tgtgactgta aagctatttg
     3961 ccccgagcgc ttctccaact ttaccgaaat ctgcatcttc taaaaataca ccaaatttct
     4021 tttgttccgt atcttcttgt tctgcttcga tgaaaccaaa agaatcattc gaaaagacaa
     4081 cattaataat tggaagctga tatttgactt gtgtaatgat atcttgcatg accatagcaa
     4141 acccgccatc accacttaag gtaaatactt gtttttctgg gtagcttagt tgtgcggcaa
     4201 ttcctcctgg cacaccatta cccatagttg cgaaccatcc agatgtcgta tgtttttgtt
     4261 taccgttcat attcaataaa cgaatagaat ggatagtcgt gttaccgaca tctgtgacga
     4321 aaatcgcatc gtcttcagca atacgattga tttctttata gacagcctct ggacgaatcg
     4381 gttcttcctc tcgatcttca aaagatttca accaatttac ccatttttct ttattttctt
     4441 gatttgctag gtaccagcta tcagcagggc gagcatcccc taactcgaca agtctttgca
     4501 aagctgtgtt tgcatcaccg aggatcgaca aagatgtttc atggcgtcgt ccaaattttg
     4561 acgcatcgat atcgatttgg ataaattcag cagttggatt aaaaaatgca cgtccaaatg
     4621 ggaagtcgct accgacaaat aagatcagat cggcttctgc caatgcttca tttccgggtt
     4681 tagtagcgac acgtccagca aaaccaagaa agttttcata atcatcagga ataattcctt
     4741 ttgctagaac ggaagcgaca acaggaactg aaaaatgttc tgagaattct ttgatttgcg
     4801 gaaatccgtt tcgcgttcct tgaccaacat aaagaactgg tttttttgct ttttcaagat
     4861 aaggcaaagc agctagcaaa tctttttctt ctggtaatat cacacttgtt ttgtgagtgt
     4921 gagcggtgga gaaaggttgt tcgtcaattt cttcaaaacc aagatcaaca gggatagtca
     4981 cgatagccac gcctttatgc tcataggcgg ctttaatggc ttcatctact acatgcggaa
     5041 gactttgtgg tgtcatgact gtacgattgt atacgctaac atctgcgaaa ataggatttt
     5101 catttagttc ctggaaagag ttatagttca tggatgtaga agcaacttgt cctaagaacg
     5161 ctactagagg cacatggtcc atctgtgcat catacaaacc attgatcaaa tgtgtcgcac
     5221 caggtcctgc agagccgaag acagcgccga ctttccccgt taattttgca tctgcagcgg
     5281 ctgcgagtgc gccgacttct tcatgacgaa cttgaatgta gttgatttcg gttttttctt
     5341 gatatagcgc atccataatt gaattgaaag atcctccggg aatgccgtaa atatggtcga
     5401 taccccagct ttcaagaact tttaccattg cgattcctgc attgattttt gacatggtgt
     5461 ttcactcctt tagcaatgat gatacataaa ttttttgtat ctagagtata gaacttttta
     5521 tcgaaaatcc caactaattc gtcttttcac aaaaatgtag tcatgttttt gggaatatgc
     5581 tagagtagta catagataga ttcaagagag gggaatggca tggttgaatg gctattgaga
     5641 cttgaacctt ggcagcaagc acttgctggg acagctttta cttattttat gacagctctt
     5701 ggtgccgggt tggttttttt ctttaaagaa atcaaaaaag aagtgctaaa cttgatgtta
     5761 ggatttgctt ctggtgtcat gattgctgca agtttttggt ctttgttaga tccggctatt
     5821 acaaaagcag aagaaaatgg agatattgct tggctagttg taagtattgg gtttggttta
     5881 ggcggtgtgt ttttatatat ggcagataaa acactgcccc atatgcattt tggaccacaa
     5941 catgaagcag aaggattgcc tacccattta aaacggacga tcttgcttgt tttttctatt
     6001 acattgcata acattcctga aggtttagcc gtgggggtag cattcggtgc agcagcaact
     6061 gcggataatc cgacagccgc tatattagcc gctgtttcgg tggcgctagg tattggtatc
     6121 caaaacttcc cagaaggagc agctgtatca atcccgttgc gacaagaagg tctgagtcgt
     6181 aaaaaggcct ttgtttacgg acaagcttct ggaatcgttg aaccaattgc tggagtgatt
     6241 ggagcgctac tagtaacgaa agtggagctg ctgcttcctt atgcacttgc ttttgccgca
     6301 ggagcaatga tttatgtcgt ggtggaagaa ttgatccctg aagcacagca gacattgtcc
     6361 agcaaacgtc attttgcagt ttttggcgtc atgagtggat ttatcattat gatgattttg
     6421 gatgtggcat taggataaat aaaaaaaata tatatgatat atcaaatagg gccgcgaact
     6481 tatatcgcga ccctattttt ttagtaagtt atatcataat gaagtgaatg gtatcataca
     6541 ttaataatag ttttttctat gtgtatttcc tttgatccag cgaacaagcc agatgataag
     6601 tgcgattggg attagaactt tgaccgcaaa ccataaaagc ccgcttagga cactgacaaa
     6661 aagactgatg acgaagctag ctgcaattag cactaaaaga accatgaaga tatttaagaa
     6721 catatttttt tgacgcatac aaaaccctcc tcttgttcat tctttactat agaaaaagta
     6781 taacagggat tgtttaaaaa cacctccaac tgaagactga tttttacttc atctacttaa
     6841 atgatcaaac aattgctttt tctctggttt atcatctaaa atagagcgga aaaggaggag
     6901 aatatcatgc ctaaaaatcg tcttgaagca tttacagatg ctgtaatcgc gattatcatg
     6961 actattttag ttttagaact tcatgaacct aacggagaca cgtgggaggc gctcgctgca
     7021 ttaggttacc gattttttat ctatattgtc agttttatca ttttagcgat ttattggaac
     7081 aatcatcatc atttatttca agttgtacat aagataaatg gacgggtctt atgggcgaac
     7141 agtttctttg tttttgcgct ttcgcttttt ccgtttgcaa cttcatggat cagtgagtac
     7201 atcaatagcc tggctccaga aatgacttat ggttttgtta ccttgaccgc taatattgcg
     7261 tattatatct tggcaaaaga acttgtcaaa gcaaataaag gccacgagaa agcattgaag
     7321 gcgatgttga ataattacaa gaaatcgtat ctatcgattg gtttgaatgt gttaggaatt
     7381 gtcttagggt atttagtagc gccagtagcc gtgctgatcg caaacatcgt gattctagct
     7441 gcttggatca tcccaagtcg taccatcgaa tcacaatatc ggtaaaatga tccgtgttta
     7501 aaatttaaga gaaaaatata gaaaaaataa ataaacgatc aaatatccga gtgatactag
     7561 tattcaaaga gggatgcaga tggatcaaaa aagaattcat gtaggactga ttactagttt
     7621 tgtcacgatg cttatttatt tggatcaagg tcatttgact tggacagtag ttggtgtcgt
     7681 aggatttttg acattattca cagcaataaa aaaataaaca tctttatgta atctgatgtt
     7741 ctatctattt aagaaaagca tgtttacagc agtatgattc tgttgtaaac gtgtttttat
     7801 ttgtggaaaa tttaattttt agaaaagtgt atcgaaaaat agatagttag tttttccaca
     7861 gtggataact tcgtaagaga taaaaataaa aaattagtca aagaagggcg tatagatttc
     7921 ttttgatagg agatatacaa cagaaatgaa taaatattaa cgtgttttca aacagtataa
     7981 aacggtgtgg tttttggaat aaaaaaataa catatctcat tttacgcttt tgttatttta
     8041 tacttaaaaa ataacgacac agaaaatacc gtgtctcaga tttctctact acttctaaaa
     8101 actactgttt ttttcctttg aaatgccttc aaaaagtagg atcaggcaga atatactggc
     8161 agaatcaagg agaagggagc ctaaataagg agaaaaacca tagaaaatat aaagtaatta
     8221 cttataaaac aagcttttta tagaaaaata atatacctga gtagggtata aattaagacg
     8281 gaattccttt ctagattgag atattgacta ggaagaatgt taagatttta agcctttaag
     8341 ctaaaaaaaa accctttttg ttaaataagg ggaatatagc ctgtcagttt caaactgcaa
     8401 gtcgtcctgc tgcaataata tagtagatta gagatgaaaa tagatttttt taaaccaatg
     8461 actgaataaa aagaccgtca tagatacaca ttggaatgtg tctattacgg tcttttttta
     8521 cgaacgattt tattgaagac ggacagttcg ggtcaaaaat aaaaaagcta cgatcagaaa
     8581 taagaataaa atgtatccca aaataatggc tagacggtct aacttatttg tcgatagatg
     8641 tttgtttaga agttgatagg cccataaacc tatcagtcct cctgttgtat tagttatcaa
     8701 atcagtgatg tccgctacgc caataccgaa taggaattga atcaattctg ccaagaagct
     8761 tacaaggaaa ataatcccta attttttaga aaaagaaagt ctcttgaaat tgacattcaa
     8821 aagcacccca aaaggaaaaa agacaatcaa gttataagtg gtctcacgta ataccgtcgt
     8881 attttcaaaa gaaaaagtag aaaaagggac gagattgaca ttgctgtaag aataagtgag
     8941 aaattgtgga agattaaatg atagcttgaa taaaatcaac cataatagaa agaataaata
     9001 aatccctaat aaaaattttg atacataccg catagacatc tcctccattt aaaaagtata
     9061 ccatcgaaaa ccgctgaaaa atcataaaaa agtatgaagt ttaaggacca acgtagttat
     9121 cgtacgaaaa ttagaagctt ttttttgttg ggaatccggt ttctttaaca aagctaagat
     9181 agcgagttgc ttggaaaagt tgctatcttt tttctgtcaa aacgactcgg aatgttcgtt
     9241 ttttgttcta atttctccaa gttgtttact ttttgatact aagtttatga aaaatcaaga
     9301 aatagttgtt atactaggta atgctgacaa acgggattgg catttcatct tggttcagct
     9361 ttttttccaa ttactaggaa ggagggatag attgttcaag acttattttc gtttcaataa
     9421 aatacaagat aatccttttc gtatcatgaa tctgatgcta cttatcttta ttgtattatg
     9481 gttaggttac ataaatcatg attttttgat ttcaagtttt tcatcattag ggatctttac
     9541 ttttttctat tatcaaaaca taccgatgaa atatttgctg aaacgtatgt ttctgatcgg
     9601 ttgcggactg ttggttgctt tcgttttggg gatcttatcg acttatgttt tatggcttga
     9661 accatttgcc gtagctttgg ttgctttttc cagcagattc gtgttgcgat tattccatat
     9721 ttctaaacca ggtggcttgt tttttgcgat gctttctgcc atgggaacga gcatgcagct
     9781 gccaatcgct caattaccga tcgtctcact ttattttttt atgggtgtcg tttttgccct
     9841 gattgcggca gtgattacaa aattactgga ttcacggcct gagcagacaa ttgaaaaagc
     9901 aactttaaaa gaaagatttc acgaagagcc tttagtgatc attgattctg ttttctacag
     9961 cgctgcctta tttctttccg tttatgtcag tcacggcttg aatctgcata atccttattg
    10021 gttgaccttg tcatgtgcat cgattttgct agcagaaaac ttggatgcga tgaagcatcg
    10081 acaagtccaa tatctgattg gatcgatggg tgggttatgt gtgtcggcat tcttgtcatt
    10141 tgtgccgttt acgcagttgc agaccatttt tctgatcact tttctatatg gaatcgcaca
    10201 atttctagtt gctagaaatt atgcagttgc taatattttt cttaatccga tggcgttgat
    10261 gctatctaca cttattcgtg gagcatactt gatttctttg atcgaatatc gttttttagg
    10321 gatcgtcata ggaagtttca tcggtttagg tgttgcgtgg gtaatgacgg ttggtttgca
    10381 acattattta acggttgttc gtcaaaaatt agaatagaag gacaaatata tgtgccaaaa
    10441 gaggttatga caagattttt gtcacaacct ctaattcaag gtgttcaaaa gccagattct
    10501 cagcattaag tcaaattcac acaaaaatgt ggaaaggaac tttcaaaaaa attttatatt
    10561 tattctatca aaaaattttg tttatgatgg taactgaata aactttcctg agatggtcaa
    10621 tgctttcctt gaaagtctta cagatattct ttcttcatag aaagtttaag ttttatagtt
    10681 agtgtacaat aaaagtatgt ttataccata tggtgtccgg ataatcagtc atttaaacaa
    10741 tgaggttcaa agatatgaaa aggggaaatt tgatgaagaa aataagcaat gaaatccaaa
    10801 ttctggcaga aactttaact cttcccaaag tcgttataga gcaaatgatt gtacaagcaa
    10861 aaaacatcct tgatgaggtg tttgagcaat atagtcatga actaatggat cctaataaag
    10921 caaaggcgac tgcaaagaaa atagacaaag tctatgaaga ggaacctttt gcagcattag
    10981 ctatttatct atatgcagct tctcaaagtt ggaaaaaaat ttatcaacca ttgaagattc
    11041 caagaactat ctatttggca acaatgaatg cttttactag atttattcat gaacatttcc
    11101 aagtaacagg aagctatcgc tttgaccgtg gtttttggat ttggcattac actagtggct
    11161 tgatctttcg gattggtgaa ttggagtacg aaatgacata ttttccgaaa gggtacacgg
    11221 atctaagatt agaaggaaag cagtgccttt ctcttcatat tccttccgat gcagatttat
    11281 ctcagaataa gataagagat agttacgaaa gtgccattcg tttttttgaa ctttattttc
    11341 ctgattacca ttaccaagtg atgtacacag atacttggtt actatcaccc aatcttacta
    11401 aatggttgaa aaaagagtca aaaatctgtc tttttgcagc cgattatcga ttattatcag
    11461 ttgatgaaca ggacgatagt ggagtccctt ggatctttgg acgagtagat gcccaaatac
    11521 atgattatcc agaaagtacg agtttacagc gacaagcaaa agagcaattg cttgcaggcg
    11581 agcacattgg ctctggctta ggtattctta cttcataagt ttagtacttc gaaatagttt
    11641 aaaagagcgt gagacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac
    11701 ggcggaagca gaagcaactc cttcggaaat aagctgaaat tcacaaaaat ttgaaaagca
    11761 attttcgtga atttcttctt atttcttgga gttaaacgct tctgtcccag cctctttttt
    11821 agtgttctat cgaacacaca gtacgttcaa taggtaaact agctataaca atagatatta
    11881 aagttaacgg acaagagggc cttttttagg tagctaaaac gtaataatga aaatttattt
    11941 ccagagatag gagtcaatca gctagtctga tcgactgttt taacaagttg actttcattt
    12001 taacttttga taccatttaa aacacaaatc gtaattgttt cgatttgtgt tttttagcat
    12061 gactacaatc aaagaagaaa gtgggtcgtc catatggaaa aaacagatat ctcaagtgca
    12121 taccgccgct tgaaaagccc aaatatcaaa acacgaaaac gtgcgctgaa gatcatcaaa
    12181 gatgtgaaac ggaatagtgg gaaaaggtaa gaagaaggaa acttaattaa aaaaatagcc
    12241 gaaaaacgtt tttcggctat tttcaataat cggtttttgt gtaaggtttc cgaaagaact
    12301 gctgttgatt ttccattttt aacatatgat aaattggtag aatctttctt ttcaaattga
    12361 taccattccc taattccagt tatatagtta actacagaac cacaaagcac gtacgtttat
    12421 aaaagtatta tctgtagtta tttttcaatt ccctttcaac ctcttatttt gaccagtagt
    12481 attgatcact ttacctttta acacctttgt tcgtttttga aacattttta gtcaagaatg
    12541 gaaacgtaac tacataatca aaggttttgc gaaatatttc atgaaatatt tcatgtagaa
    12601 gcgagcaata cggttgcaaa taaccattat cgtttaataa gcagttacga aagctcccaa
    12661 tatctctaac gttcagctca ctccatttct tttcttcaaa aaaatcagtg attcgtatct
    12721 ctttctattt gtcgaaaaag ttaactttaa aaagtaacgc cgaaaaaata tgcaaaaaaa
    12781 taaaaaagca gttactattg ctagaataac atcaaggaat aataagtttt ggttttcaga
    12841 taatagtcga taagttgtgt attggaattt tagatacagt aataaagtat ttttcatgaa
    12901 attttgtgag aatcactatt acaagtatct ttaataaaat taatggttaa ggaggaactt
    12961 aaagtagaat gtaaatgaat agttgtagtc agtcgattgt gatactagac tcaaagttgc
    13021 aggaataagt ttttcaattt gtgattggaa gataaaaaat gaaaaagaag ggcttttttt
    13081 tatactttgt tgattgtagt gtttattttc cctctgttta gccaagtaac attttctatg
    13141 actcaaccag tttcaagtga ggaacatgat aacctttttc gtgctacaga agagtttaag
    13201 aaatatcagg aaaatattgt tccggagact ccattagcta gtgagccaat tactgatatt
    13261 gatggaaacc aaataggatg gtatatacag tacgaagtta tggtggtaaa taatgactca
    13321 atagtaaaaa atacagtagg cttagagcta acaccgattg aaaaggtgag ttcattgtta
    13381 acgtatattt atactgatga aagtgacgag actgagatca ttttaacgga ttactcaaaa
    13441 attgctactg ataaaaagta tatattgtgg atgtagacac tagtgaaata ctttatgata
    13501 tagaaattga tgaaaattta ctagatgtag cacaagaaat cgatcatcaa aagcaggaag
    13561 ctattcgata tgctggtaga aaagggaaaa gaaaatattt attacgtgaa tgtagcaaaa
    13621 gttagagaag atgaaaatga gtggaaagaa ttcaagagtc gctactcaat taactcgact
    13681 ccgacattta cagtttacag agaaggatcg atagaaaaaa ctgttttttg gactaaagaa
    13741 agtggaatgt ctttagctga agtagaagag tttttggatt atgttagtat gcaacagtaa
    13801 gggagttctt attatgaaaa attttgacac atattatgtt attgtttttt cgatatcttt
    13861 gctgttaata ttactaactt taatgcagta taaagagtat ccagagtatt ttattttaac
    13921 ttttgtatat ttagtaagta gctacacact tctatataaa ttcagaagtt cttctagagt
    13981 ttctaaagtt tgtttcttaa ttagcttatt gattctataa tatttttgat ttatatagga
    14041 aattaaaaat attatagaat caacactcag agaacgggtt ggtttttaat agatttaata
    14101 agtactgata atgaaatttc tcctggttgg gaaaacgaag ttggttaccc tgtagaagaa
    14161 ttttttcaag attactttgc ggagtatgcc gataggataa tgagagatta cgtaacagat
    14221 aaaactcaag aagtcatcaa tcaatcccag tcattaaatg aattatctga taaggcgaaa
    14281 gaagaagcag ttgccattat ttcttcaggg attagtgact atattgatat gagtggtgaa
    14341 atcaattatg gcgataaaga caaaattgta cgcatgataa aagcttttat caaaactttt
    14401 gatgtgaagg cagatcttaa gtttaatgat acttacctag taggaaaatt aattaatatc
    14461 cttgggacag agaaagatac ggaaacgttt atttcctctt tgtctatgtt gtttacagat
    14521 aagcattttt caactcagct tggtggagag gaactagtgg aaatctttta accattacgc
    14581 ccagcattaa taaaagtata tcaaaaaagt actcctgatg atttttaaga ttggtttgag
    14641 aaataatgtg aaaacactat ttttaatgtt aattatagat agagatgaac gcgtcatatg
    14701 gttaaaagaa tgaaaaagtc aaagatacac tgctatattt cactaaggat atagcagtgt
    14761 atctttcatg tcgtttcact ggcagttttt tataaaagga ggtgtgcatt tcagttgctt
    14821 ttctttggta gttagttgaa tcgtttaaga attaacggat gtggcagaca ggtgctctgt
    14881 ccaaaaacca gtaaaatctt tgatggttcc atctttattt agctgtgcct gttcaaatac
    14941 tgttttgttt ttaccgaatg gatcaattgc aataattgtt ccgatccccg cacgtttggc
    15001 agcaagtaat cctgagtaag catcttcgat aacaagacaa tcagccggag ctaagctcag
    15061 tttgtctgcc gctttaagaa aaatatccgg ttgcggtttc ccagggaaag agccgtcgtc
    15121 atagacaatc ttgtcccagt caaaccagcg ggctaaatcg aaaatatcaa aataaaaagc
    15181 aacattctct ttgacagtag ccgtggcaat cgttaatgga atctgttggt cagtcagctg
    15241 gtcaagagtc gcttcaagcc cttttgtcaa ctgaagctcg ttttcatttt gcaagcaaag
    15301 catacgataa tagctttctt tttcttctga caatacggcg atctcagagg cggtcaaatg
    15361 ctctgaaatg aaatgtttca aaatctccgt attcgtccga ccatgaatat tttttaaaat
    15421 atcctcgtct gataaaccat cttttgtatg atcatgtatc atatgcagcc aagcagcttc
    15481 atgcagatga gagtccaaaa acattgttcc gttgaaatca aatataatcc ctttcatagt
    15541 tacgctccta tagtggtatt ttcggttgaa tcagcaattt gcagatcgac atattgtgca
    15601 tagtgctctt gaacctcccg cgagatctgg cgatccgtaa tgagcagatc gaaatcatgc
    15661 aaattataaa acacataaaa atcatctcga ttgaattttt tattatcaac gagtagatat
    15721 ttcttacggg aattatttag agcgatctgc tgtgcttctc cttcttcggt actgtaagta
    15781 gagatcgctt cgttgtgaac gccatttgaa ctgataaatg cttttgtaaa ctttagtctt
    15841 cttaaattgt cgttggtgat cggtccgaca aatgcgccgg tatttttccg gaaatcaccg
    15901 ccaatcagta gaatttctgg tccatctgct tgagcaagaa tatcaaaaac tggataatta
    15961 tttgtaatga tccgaatttt cttgttcagt aaatgcgtcg ccaatagttc gattgttgta
    16021 cctggtccta agaaaatcgt ttctccctcg tgaatcaagg aggctgctgt ggcagcaatt
    16081 tctttttttt cttgaatctg cagagtggct ttttcagtgt gagaaagttc ttgatctaaa
    16141 gtgtaggaaa gactttgagc ccctccatgg acacgcagca gcttgcccga tttttctaat
    16201 tcatctaaat ctctgcgaac ggtcatatca gaaacatcta attgattgat gatttcatta
    16261 acggtaagga ttcctttttt attgaccatt tctacgatag taagcaatct ttcttttttc
    16321 aacattgtct tcacctctaa tttaaggata atctatcata taaacggaca acagtcaatc
    16381 taattcgaac aaaagtaaac atttagggtg gtttttatgt ttgttacaat agattttatt
    16441 gctgttttct ggtttttatg attatttgtg tttgtttttg ttgacaaatg ttttggatgc
    16501 gtttacaatg caatcagtta gaaagagcga aaactgatta tagatttgag gagagatgta
    16561 catgcttaaa ttaccagaag attttatttt tggcggagcc acagcagcct atcaggttga
    16621 aggagccact aaggaaggtg gcaaaggcgc tgttgcttgg gatgactttt tagaggaaca
    16681 agggcgattc agtcccgatc cggcaagtga tttttatcat caatatgcaa aggacattga
    16741 attgtgtgaa cgttttggtg taaatggtct aagattatcc attgcttgga gccgcatttt
    16801 tcctgatgga gctgggaaac caaatccaga aggaatcgct ttttatcatc gtgtgttcga
    16861 agaatgtaag aaaagaaacg taacgccgtt tgtgacgttg catcattttg atacccctaa
    16921 acgtttgttt gaccatggag attttttgaa tcgagaaaca atagaagcat ttgtttccta
    16981 tgccattttt tgttttcatg aattcaaaga agtcaaggtg tggagtacct ttaatgaaat
    17041 ctatccagtt gcaaccaatc aatatttgtt aggtgttttc ccaccaggaa tcaaatatga
    17101 ttttacaaag atcgtcgcct gtcttcataa tatgatggtt gctcatgccc gtgtagtgaa
    17161 ctatttcaaa gaaaacgaac ttccgggtga aattggtgta gttcactcat tagagacgaa
    17221 atatgcggca acagacgcgc cggaagataa gcatgctgcc tttttagacg atgcattgtc
    17281 gatccgtttc ttattagatg ctacctattt aggctattat tcaactgaaa ccttgactgc
    17341 tcttgatgag atatgtgaag caaatcaggc cagctatcat tttcctgaag aagatttcgt
    17401 tgaattgaaa aaagcatcca cgcgtaatga ctacttaggg atcaatcact accaatgcca
    17461 ttttgtcaaa gcttacgatg gagaaaatgc aatccatcat aacggtacgg gtgaaaaagg
    17521 gacctctgtt tataaagtga agggaatcgg agaacgtatt tataaagaag gaattcctcg
    17581 aactgattgg gactggctga tttatccaga aggtctatat gacttgttgc tgcgaattaa
    17641 aagcgattac ccacattaca acaaaatcta tatcactgag aacgggatgg ggtataaaga
    17701 tcagtttgaa gatggcatta ttatggatca gccgagaatc gactatttga gagtctattt
    17761 ggaatcctta agcaaggcga taacagccgg ggtaaatgtg aaggggtatt ttttgtggtc
    17821 gcttatggat cttttctcat ggacaaatgg gtataacaaa aggtatggct tgttttatgt
    17881 tgattttgaa acccagaagc gttacccaaa agaatcagct tactggtaca agttggtcag
    17941 tgaaaccaaa acgattattt aataagtgat aatggaggaa gaaaagatga ataaagagga
    18001 attacaaatg cttggttttg agatcgttgc atattcaggc gacgctcgca gtacattatt
    18061 gaaattattg aaagaagtaa gacaaggaaa ctttgaacat gttgattcgg cgttgaaaga
    18121 agccgacgaa aatttaaccc ttgcacataa ttcgcaaaca aaaattttag cggaagaagc
    18181 atctgggaaa gagatggaga tgggattcat ttttatccat gggcaggatc atttaatgac
    18241 cactttattg ctgagagatc tgattcaaga ctttattgta ctgtacagac agaatcaagg
    18301 ataggagaag aacgctatgg atggaatcat taaacaaatc gaaaaaggga aaccattttt
    18361 tgaaaaaatc tcacgtaata gttatttagg agccgttcgt gacggatttt taacagcgat
    18421 gccagctatt ttattttcaa gcattttcat tttgattgct gcggttcctg agatttttgg
    18481 ttaccaatgg cctgaatctg tctccacatg gctatggaaa gtctataatt attcgatggg
    18541 tgttgtaggg ttactcgttt cagcaaccac tgctcgctgc ttggctgatt cgatgaatcg
    18601 tcgcttaccg aaaaacaaag taatcaacac cacctctgtg atgctggcct ctgtggtaag
    18661 tttcctgttg ttaagcgtct ctccgattga aggtggattt tcaacggaat atatggggac
    18721 aaagggacta ctggcttcct ttgtcgctgc atttttaaca gttaatttat ataagttttg
    18781 tgtgctgaaa gatattacca ttaaaatgcc taaagaagtt cctggaacga tttcgcaaac
    18841 cttccgagat atttttcctt tctctttttc agtatttgcg gccgttttaa ttgatttagg
    18901 cattcgacaa atcttccaaa tctcatttgc agaagcaatc attacggtgc tgcagccgct
    18961 atttacagca gctgacgggt acttaggaat cgctattatc tggggagcga tggcgctgtt
    19021 ttggtttgtc ggtgttcatg gaccgtcgat cgtggagccg gcaattgcgg caattatcta
    19081 tgccaatgtg gaaaccaatc tgcaattgtt caattccggt gaacaagctt ctaaagtact
    19141 gacggtgggt ttagggaatt ttgtaggaac tatgggagga actggggcaa cattggttgt
    19201 tccttttatc ttcctactct ttgctaagtc taagcagttg aaggcagttg ggaaagcttc
    19261 ctttattcca gtaagtttcg ctgtcaacga accattgcta tttgcggcac ccatgatttt
    19321 gaacccttat ttttttattc catttttgtt aacacctgtt gtaaacgttt gcctgttcaa
    19381 gttttttgtc gatgtattgg gcatgaacag ctttatgtac gttttgcctt gggcaactcc
    19441 agcaccgatc ggtttggttc taggaacggg catgggtatc ttagcttttg ttctagcagc
    19501 ggttctaatc gtcgttgact tcctgatcta tctaccattt tgcaaagcat acgatgctgt
    19561 cttagtagag aaagaagcac aaaaagcaga ggtgatcgca accgaggaag gtgcttctgc
    19621 cgatgtacgt gaggaagtac ctgtggcagc gagtcaagtt atcagcgatg caaataaaga
    19681 aatcaatgtg ttggtacttt gtgctggcgc agggacaagt gcgatgttag ctaatgcttt
    19741 aaaaaaaggt gcatctgaat cgaaagcacc gatcagtgca atggcgggtg cttatggttc
    19801 tcattacgaa atgctgcagg attttgatat ggtggtctta gcaccgcaag tcaattctta
    19861 ctatgatgat atcaaagaag acaccgatcg cttaggaatc aagctggttg cgacaaaagg
    19921 agcagaatat atcggattaa cgagagatcc tgaaaaagct gttcagttta ttctgaacca
    19981 atttcaataa aggaggagcc ataatgaaaa tgggaatttg gttgcgtaaa gctgttttat
    20041 ttgcagctgt gttaggggct ttgggggctg tttcaaaaac tgttgaagca tctgccagcg
    20101 aatttattcg cggagcagat atttcgatcc tgtcagatat ggaaaaatct ggtgcaaaat
    20161 attatgaaga cgatatttca aaagatgcct tagagatttt aaaaaataat ggtgtcaact
    20221 atgttcgact gcgtttgtgg caggatcctt ataatagtaa tggagaaagt tatggagcag
    20281 ggacaaatga tctggaaacg acgattgacc ttgcaaaacg agcaaaaaat ctgggactta
    20341 aggtgctgct ggattttcac tacagtgatt tttgggttga tcctgggaaa cagaatcttc
    20401 ctaaagcatg gcaaggtctg acctttgaag aaatgaatac ggcattgtac gactatacaa
    20461 agaacgttct gtcggaaatg aagcagctgg acgtttatcc tgatatggtt cagatcggca
    20521 atgaactgaa tagcggaatg ctctggcctt atggcaagtc ttggggagaa ggcgggggag
    20581 aatttgatcg attggctgct tttttgaagt ctggtatcca agctgttcga gacactcaac
    20641 ctaaaacaac tccagtaatg ctccacttag ctgatggtgg cgatacagga gcatttactt
    20701 ggtggtttga tgaaatcacg agtcgaggtg tgtcatttga tttaattgga gtgtcgtatt
    20761 atccgtattg gcacggttca atgaatgaac ttcaaaccaa tatgaatgcg atcagccaac
    20821 ggtatggtaa agaagtgatc gtagttgaaa cagcttatgg gcatacaaca gcgaatgccg
    20881 atactatgcc aaatgctttt ggtgaagccg aagcagctgc ggggggatat gaaccaacac
    20941 cgacaggaca agcagagtat ttgctggatc ttgccgatcg gattcaggca gtaccgaata
    21001 atcgcggggc gggcttcttc tattgggaac ctctttggta caacggaaat gtgagttggg
    21061 caacacaagc aggtatgaat tatctcagtg ttcaatccac tatgggaaac gaatgggaca
    21121 accaagcgat gtttgatttt gctggaaacg ccttgccaag cttgcgtgcg tttaaacaag
    21181 caggggcgca aataaatcta gttaaaaatg caagtttcga aagcgatgga ccaacgacca
    21241 gtccttccaa ttggcaaacc tggtttcaac aaggaaccgc taagggtacg atcaaaacgg
    21301 aagcgggcac tttttttggg gactacaaac taactttttg gcaggatcaa gcatacgagg
    21361 catcagctta tcaaaatgtt agtggcataa gcaaaggaac ttacacattg agtgcctatg
    21421 taatgtctgg gggagagttt gatactaatc agttatatat caaaaattat ggtggagcgg
    21481 aactaaatac tgcggttgtt aaaacaagtg aatggacaaa aattacaatc aaagacattc
    21541 caattacaac aggtgtttgt gaaattgggg tttatagcaa ggcttcagga aatgcttgga
    21601 cgaacgttga ttttgtttca ttggtgaaac aatagactgc ggatcgtttc gattcaagat
    21661 cggaggttcc cttcagccac atcgttagcg tagaaggcag gaatcgaacc tgcacctcgc
    21721 ctttgccaaa aataaccaga aacgatttcc agtcattttt accaaaacga gtaaagcacc
    21781 agacttcatc tacactttgt attatgctaa agagagttca acgatttcgt aagaagcaat
    21841 gaaaaaattg caaaaagttg tgcagataac ataaagtttg tttaagaaag ggtaacctta
    21901 gaattccgtc gtgaatatga tttgagcact tagatggata gaaaaaagtt ctcggaaaac
    21961 agttcgtttt tcgagaactt ctttttatga taagaatgaa acaaattaac ttatattata
    22021 gtctcttttg gtagggaacc atcttatttt taatgaaaat caagcatttt agctattgaa
    22081 gaatgttgat ttatagggat tccccctcca tattatgttg gctctttatt cccactctgt
    22141 tttcaacgta aagtgtataa aacagaatgc gatactatca agggagttaa aacaatatac
    22201 agaacagtaa aacactataa ataaaaataa aagtgcaata aaagtgcaat ttttgcacct
    22261 aatcatactc tgtttatata tgaaaactag attctgaatc caatatttgg ttaacgagag
    22321 gcgttcgaat ttgaacgtct tttatatatt actttcaaat aaatatgagg tagaatacta
    22381 tctattttct ttttaaccca attgttataa tggagagtgt aaaatatttt gtgtaaatag
    22441 aaaaaaggaa gtcccttctg tagaatagag ttaccacaac acattcacag aagagaggac
    22501 ttccctatga acgattttac tacagaaatt ctaaagactc tagcgaacaa aggcgatttg
    22561 aatgaattat tccgtgtcca tttggagaaa gcagtcaata cgcttctcaa aacggagtta
    22621 acggctttcc tagattacga aaagtatgat cgcattggtt ttaacacggg taattctcgt
    22681 aacggctcct atgaccgtac ggtcaagacc gagtatgggg aacttcatct ccagattccg
    22741 cgcgaccgca atggtgagtt caagcaacag actgttcctg cttatagacg gacgaatgac
    22801 acgttagagg agaccgtcat tcacctcttc cgaaaaggta ttaccatgtc ggaaatcgca
    22861 gacttgattg agaaaatgta tgggcattac tacacgcccc aaaccatgtc caatataaca
    22921 aaatcattta cagaagaggt aacggcgttt aatgggcggg agcttcatga ccgttatgct
    22981 gctatttata tggacgcaac gtatattccg ttaaagcgga aaaccgtcgc caaggaagct
    23041 attcatatcg cagttggcat tcgcccggac ggatcaaagg aagtattgag ctatgcgatt
    23101 gcaccgactg aatccatcac gatttgggag gaaattttat tggaccttca agagcgcggt
    23161 ttgaaaaatg tcctcctgtt catcacggat ggcttaaagg ggatggtagg agcgattagt
    23221 cggttctatc ccaaagctcg ttttcaacat tgttgtgtac acgtttcccg taatatcagt
    23281 cataaagtgc gtgtcgatga tcgtaaggaa gtctgtgatg attttaaaat ggtgtatcaa
    23341 gcctcatcta aagaggtggc gttggaagca cgtggtactt ttgcggaaaa atggaaaacc
    23401 agctatccaa aagtggttga atcgattctt tcgaacgatc acttgctcac tttctatgat
    23461 ttccccttgg ccatacgcaa gagtatttat tctacgaact tgattgaatc ctttaataaa
    23521 caaatcaaga aatacagcca ccgcaaggaa cagttccaaa atgaagagtc gatggaacgt
    23581 ttcttagtct cgtcttttga tacttacaac caaaaattcc taggtcgcag tcataaaggt
    23641 ttccaacagg ccgaaggcga gctcgaacaa atgctaagcc aactgattga gaattagaaa
    23701 caatctgcag tagggaagaa tcatttacac aaaattattg acgctccctt ataatgagaa
    23761 cttttactaa cataatttat gataaactta ctatataatc atattaatta aatagttagg
    23821 agatctgctt atgtttatat ctactcttca aattcgaaat tttagaaatt atgaatccga
    23881 aatctttcga ttcgataatg agacaaatac tattataggt gagaatgatt cagggaaaac
    23941 caatgctctt actgcattga gaatcttatt agatgatagt tattactata gtagtaaaac
    24001 actaaaagaa tcagattttt ttcatggaat tcaaaatggg tggcaagggc attggattat
    24061 tatctctgcg acatttgaag gaatcagcga agaagaattt gacaatgaga tttgtgcttc
    24121 catttcttta aattctgaaa gtcaaacaat cttagaagaa ttgatttcaa atgcagataa
    24181 aggtgtaggt agtatttctc tatttattag acctaacaaa gctattagaa agcaattatt
    24241 tgatatttcc actgaattag actctcatca atttaacgaa tttagaaatt ctattcgtct
    24301 atccgactat gaattttact acacatctaa atcaaatcta aatttctgta tcaatgaaaa
    24361 ttatgataaa ttggtaggaa gactcaatga caatatagct agtaatcctg aagaagacga
    24421 tgaagctctg ttgggctcaa gaattgacat gtcagatatt ttcaagcata tttcggttgt
    24481 ttatgttgac gctcttcgag atgttcttag agaaatgaaa aacaatcgaa acccagtcaa
    24541 aagaatcatg gaaacaattg aatcaaaaat atcatcagat aatgttgagt cattaaaaac
    24601 aattatacag caattaaatg agactattac cagtgttcct gaaattagga aaattggcga
    24661 aaatattaat cggcaactaa atagcataat tggttcagtg tattctccta atcttcttct
    24721 ttcctcaact atgtcagatg atatgggttc tttagcaaaa tttctttcaa tgaagccaga
    24781 acaaaatatc gacttagact tactagggtt aggacatcta aatatgattt atttagcttt
    24841 gaaaatagtc gaatttgaag cgtgtcgttc aagagaacta cttaatatta tgttgattga
    24901 agaaccagaa gctcatattc atcatcatat tcaaaaaact ctgttcgaag gattaaatct
    24961 tcaaaaaaac tatactcaga ttctcatgac cacacattct gtacatttag ctgaagtgtc
    25021 tgaaatatct cggatgaatg tattaaaatc gtttaatggc aaatctatcg ttatgaatcc
    25081 agcaaataat ctgaactcat ttgctaaaga aaagttaaaa aagaacaatc taaatctaat
    25141 aaacgcagta gagagatatt tagatgttaa aagaaatgga ttgctttttt ctaaaggtgt
    25201 tatcttagtt gaaggagatg ctgaagaaat acttattcct caaatagtaa aaaaattatt
    25261 tggtataaac ttagacgaac ttggaatagg tttgattaat gttggtagta cttcgtttga
    25321 atatatcgct agcttgtttg acgatgatag aatacagaga aagtgtgcta ttgtaactga
    25381 tttagatgaa caggcaatcc catcagaaca tcgattgtat aaaagcactg cacaagagaa
    25441 aggtcaagca agaaaagaaa aattatcctc cctttttaat gaaaacaatt gggttaattg
    25501 tttttatgct aatacaacat ttgaaattga gtttttagat gtgaataaag aaaatattct
    25561 aacttatgtc agtccaatta tagatgacgc attcaaagat aaaaaaacaa taactgctta
    25621 taaagaagaa atacaaagtg aacaatattt agaaagaaat ttagcgatgt taaaactagc
    25681 caatcatgtt ggtaaggggt ggtttgcaat tgagttaggt gaaaaaattg actttttaat
    25741 caaaattcct aagtatattc ttaatgctat tgcttttgca tgtcaggaaa tagtagatct
    25801 ctcaatttat aaaaaaattc tactccatac aatcgacttg tacgaggaaa ctacagaatt
    25861 gtcagcttta tcagctagtt taaaaacaag tgatacagct gatttgaata tagcaataat
    25921 ttctttatta gagtactttg gtaaagatga ggatgttgtg tatctaatca atcagataga
    25981 aaattacaca aaaatacttt atgctgaggt ttcaaaatga caaatatctt cgaaaaaatc
    26041 gtaaatgatt taaatttaga acaaaaagat gttgtggaaa gtgaagataa cctttatgta
    26101 aatgcatgtc ctgggagtgg gaaaacaagg gtacttacaa ggaaaattgc ttaccaagca
    26161 attaaacacg atggatcact aaaaagaata attgctatca catacactaa tagagctgct
    26221 gaagaaatta aggaacgcct aagtttactt agtatagatg atgatattaa tatttgggtg
    26281 gggacaatac atcagttttg tcttgaattt attatttatc cttttaaaat gaatctctca
    26341 agagtatcaa aaggatttac aattattgat gattatactc aaagagctta cgtcagccaa
    26401 attaataata tgctgaattt aaatctcaaa acgtatgaac tgaacaaaat tgatttgtca
    26461 ctttctcatg attttaaaat taatgaagtt cagtatactc aagtttctga aatgtatcac
    26521 actatgttgg aaaaaaataa agaaattgat tttgatatga ttttactagt agcgtataaa
    26581 attttaactg taaacagtaa cgttgcacgt aatattgcta gtaatataag ggcgatttat
    26641 gtggatgaat ttcaagatac tagagaatta caatacaatg taatagctaa attacttcaa
    26701 aacaaccctc aaattcagtc aatgtttgtt ggagatattg accaagcaat ttatggatca
    26761 ttagatggta tagctaaatc agttgaagaa ctagaaatgc taactagtca cacttttcaa
    26821 tcaaagacgc tacatggctg ttacaggtct aatcagagat tagtagactt ctactctaat
    26881 tttcagagtt gtccttacga gattgaaagc agagggaata ataaaaacga ccgtggtttt
    26941 ataagttatg attgcaaagt tactaaggag gatttaccca acataataaa aaatataata
    27001 aaggaaaaga ttgaaagcgg cattcatgaa aaagatattt gtgtacttgc tcctcaacac
    27061 tatcctctgt tttcactggg tgagtattta aagaaagaat taccgtattg caattttgat
    27121 gctataaata tatcaccaat taaggtaaat aatcatagtt tatttttcaa gttagcaatt
    27181 ttgtacttta ctgaatcagg tgaaaaggtt cgaagaagaa agctaattgc aaacgatatt
    27241 ttaattatat taaaaaatga atttggaata gaagttaaag attattttat tgacttagat
    27301 ttattacagc taataaacag tgtgaaaaga atgtatagtg gagacgacaa cggtgttgat
    27361 ttatacaaat ttgttaccaa acatatattt gttaatctta atattgacga gaaaaattac
    27421 ccgtctattt ttaagcacta tctatttttt attaacgaag tagaagatcg gataaaaaat
    27481 aattcattat ctagtagtgt atctagtttt aagaagatgt ttaaatctcg agatggtatc
    27541 acacttacta cagcacataa agttaaagga aatgagtatg atactgttat cgctattaat
    27601 cttctaaacg ggaaaattcc tcattggaat gaaattttta atgtttctga taaaggaatt
    27661 agttcagcga gaaaactact atacgtaatt tgttcaagag caaaaaataa tttatttctt
    27721 ttctcagagg tgggttattt tacgagtaaa ggagacttgc tttatcccac tcaattatta
    27781 gataaaatag aatttgatta taatatttaa aatacctagg tagtcgttgt tttaaatata
    27841 gtaggaatcg gacgctcagt acggagcgtc tattttttta cccaattttg aaaggaagtg
    27901 aacttatgaa aataaaaaat caagaatcaa aaggtcgctc tccccccttt aaaaccatca
    27961 cacaaataag attcattcaa acataaaaac agaaaggata ggtaaaaata tggaacttaa
    28021 atatgtcatt cccaatatgg aaaaaacatt tggacaatta gaatatgctg gtgaagggaa
    28081 cattgaacaa cgtcgaataa atggcagacc aaccattctc tctcgtagtt acaatttata
    28141 ctcaacgatt caacgagcag atgatattgt tgtcattctt ccctccgaag ctggcgagaa
    28201 acattttgaa tccgacgaaa aagtcaaact catcaaccca catattaccg cagaaggtta
    28261 taaaattggc aatcgaggat ttaccaatta cattatgcac gcagacgata tggtgaaagc
    28321 ataagaaagg aaaggtgaaa aaatatgaga ttagcagaag gtattgtgat tgaaaaagac
    28381 cagacatttg gcacattgaa attttccgcc ttgcgtcgtg aggttcgtat ccagaatgaa
    28441 gatggtacgg tttccgaaga aattaaagaa cgtacctatg acttaaaatc aaaaggacaa
    28501 ggacgcatga tacaagtcag tatccccgct tctgttcctt tgaaagaatt tgactacaac
    28561 gcagaagtgg aattaatcaa tcctgtggca gataccgtag cgacggctac ttttcgaggg
    28621 gcagacgttg attggtacgt gaaagcagac gatcttgtat taaaaggaca gacaaaacct
    28681 catcagaaat ataaaaaaga agcgtccgaa agtgaaaaat aggtgttgct tatgacaaac
    28741 acaccattga ttttgaaaga aattccgaag gatgaagcca tttcttttat tcggcaatac
    28801 cattattcaa agatactccc tagattgtgt aaatactttc tagggatttt ttcagaagaa
    28861 aaattattag gcgttgtgga attaggctgg ggtacgcaac ccctacaaac catacgcaaa
    28921 ctctttcccg acagttccct tcaaaccaca gattatttag aaattggaaa gatgtgtttt
    28981 cttccagaaa tgaatcaaac caattatttt ggcagtcaag ctctttcagc tctaattaag
    29041 tggttgaaag aacatacgga ctgtcatttt ttatatacac tggcagatgg aattgaggga
    29101 aaatgtggct atgtctatca agcgtccaac ttcttttact gcgggtattt caagacaagt
    29161 gtctatcgtg ataagcagtc atgggaaaag atacaccctc gaagtgctcg ccttttatta
    29221 gaggaaaatg ctcgctttga acaggtagag aaaaagcatt ggctttccca ggcattttgc
    29281 gaatacaaag ggattgagaa aatcaatgga cgcatgtttc gctatctcta tccgttgaca
    29341 aaagaagcga aaaaactgtt aggacacact ctctatcgac gtcattatta tccgaaagaa
    29401 aaaaatttac gctttgaaaa gcgaatcgct tatcagaaat atgaagccat ttctcaacca
    29461 acttttgata aacaggctcg tatctacaat acacaattat tttgataggt ggaaaggagg
    29521 atttttatgc cattgcttca tgacagagga gagcgaatca aagccaacga taagcacttg
    29581 gtctatcatt ttgtactaat ttcattgctg gtagtttttc tagtcgtcct actccctttt
    29641 cattggagag cgttacaatc ggtgaactgg caagcagttc agttagatac atttttctat
    29701 caacttcata cgccatactt ttggagcagt attctcactg ctctttttgt atgcggactt
    29761 atcagctggc tttattatca ctatcgtata gattctataa aacagttaga gcaccgtcaa
    29821 aagttagctc gtatgatttt agaaaatcat tggtatgaag ccgaagaaac tcaatcagag
    29881 ggattcttta aagatttacc cagcaagaaa accaaacaac gaatccgcca ttttcctaaa
    29941 atatattatc gaatgaaaga tggactaatc catattcatg tagaaatcac cttagggaaa
    30001 tatcaagatc agcttttgca cctagaaaac aaactagaaa caggattgta ttgtgaatta
    30061 gtggaaaaaa cgttgaaaga ttcttatgta gagtatgtcc tactttatga tactattggc
    30121 aatcgaattg gcattgaaga cgtggtagct caagatggac gtgtcaaatt gatggaatcg
    30181 gtctattggg agttcgattc tctccctcac atgttaattg ctgggggcac tggtagtgga
    30241 aaatcctatt ttattctcac attgattgaa tctctcctgc atacaaatgc aaagctttat
    30301 attcttgacc ctaaaaatgc tgatttggca gacttagggg cggtcatgaa taatgtctat
    30361 taccgtaaag aagatatgtt ggcatgtatc aatcaatttt acgaagatat gattgatcgc
    30421 agtgaaacca tgaaacaaca ccctaactat aaaacaggag aaaattatgc ttatcttggt
    30481 ttgtctgcca attttcttat ttttgatgaa tatgtggcaa tgatggatat gctaggtcgt
    30541 gaaagtacca ccgttattca taaactcaaa caaattgtaa tgttaggacg acaagcaggc
    30601 ttctttttaa ttcttgcctg tcaacgtcca gacgcaaaat attttagtga tgggattcgg
    30661 gacaacttta acttccgtgt ggctttgggt cgtatgtctg aaatgggctt tggtatgatg
    30721 ttcggaagcg atacacaaaa acaatttttc ttaaaaccca tcaagggaag aggttacgtg
    30781 gatacaggaa aaagtgtgat tagtgagttt tatacacccc ttgtaccaaa aaggtacgat
    30841 ttcttaggag aaataggaaa agtcattcaa aaaaagcaat ctgagcctgt accgcacgaa
    30901 gtgaaaggta caggttcaga cttgctttga ttggtgtggc gttagccacg ccaatccttt
    30961 agccccctct atctaacagg ggggcacaaa tacaactgaa aacaggcaat tctcatcaac
    31021 caatgttgat ggtacaaagg attgcggaaa atatgaaagg tgtaaactca agggtcatag
    31081 tttacatctt ttttagattg gaggtttgcg aatggaaaac gaaaaaattt ggattaagga
    31141 attgaaagaa aagcgactgg cttatggcgt ttctcaaaat aaactggcgg ttgcttctca
    31201 tattaccaga ccttacctat cggatattga aacaggcaag gcagtcccta ctcaacaggt
    31261 gaaagaagat ttactgaacg ctttggaacg cttcaatccc gataacccat tggagatgtt
    31321 attcgactat gtacggattc gattccctac caatgatgtg gctcaaatta tcggtgaggt
    31381 tttacgtttg aatatggatt atatgctaca cgaagacttt ggctattatt cttatcccga
    31441 acactatcgt ttcggtgata ttgttgtcct agtctctcat gatgtaagta aaggtgtttt
    31501 attagaactg aaaggaaaag gctgtcggca atttgaaaac tttctattgg ctcaacatcg
    31561 gagctggtat gatttctttc aagattgcat ggaacataaa ggtattttca aacgcttgga
    31621 tttagctatc aacgataaaa cagggatttt gaatattcca gatttggcaa agaaatgtaa
    31681 acaggaagaa tgcatttccg tctttcgaag ctttaaaaat tatcgcagtg gtgaattggt
    31741 tcaccgtgat gaaaaaccat acatgggaaa cacactttat attggctcat taaaaagtga
    31801 agtctatttc tgtatatatg agaaagatta tgagcaatac gtgaaaaatg atgttccctt
    31861 agaagacgca gaagtaaaga atcggtttga aattcggtta aaaaatgacc gagccaccca
    31921 agccatgaaa gatttattgg cacatcaaca ggcggaaaaa acagcctttg aaattatcaa
    31981 tcgctatatc cgctttgcag ataaagacga cactaaacgt cggagtgatt ggaaaactaa
    32041 tgaacgttgg gaatggttta tcggtaaaaa tcgtggagca ttacgcctaa ccacacagcc
    32101 agagccttat tcttttgaac gaacattgaa ctggttacat catcaagtcg cccctacatt
    32161 aaaaatcgca agcattttag atgttctcaa cggtacaacc attatctcca ccatgattca
    32221 ggaagccaaa ctgacagaaa aacatgagaa attaattgaa caacaacatc tagccatgga
    32281 ggatttgatt acatgagcct tttaacaaaa gaattaaaga agttagggtt tcagtgtggc
    32341 attgagtttc aagcgtatat acaaaataca gggaaatata cgtctctcat tattgaggga
    32401 aaacggcaag ctggtgatac catctatacc tatgattttt acaaggtgag attttataat
    32461 aattatacca atcgagtaac cgtctatgga gagcatttaa ccccttttca attattaagg
    32521 cgtgtaaaaa gctatatcta ttatcgagaa aaatatctta aggaaaggag gacaatcaca
    32581 tgaattttgg gcagaatttg tacaactggt tcttggataa tgcacagtca ttggtattgt
    32641 tagcgattgt tgtcattgga ctgtttttag gttttaaacg agaattttca aaactcattg
    32701 gcttcttgat tattgctcta atcgccgtcg gacttgtgtt caatgcagct ggcgtgaaag
    32761 atgtgttatt aaacttattt aaccgaatca ttggtgctta acagatattt ttcatgataa
    32821 aataggaagt atatattttg ttatgaaagg atttatgaat atgtttacaa atgatcaatt
    32881 taaatctctt ttaattaaaa cattagaaag aaagagctct tcaaattatt atgaaggagg
    32941 taacgagatt gtttctaaaa tgaaaatatc agaggtaact ttagatgaga ctgacgattt
    33001 tacttattat aaaggatata aaaagggctg gaatacgctt tgcacatatc ttaaaataag
    33061 agttcctttt gatgatttag acttttttga aagtcataaa gatttgatta cccagacagc
    33121 aagctccata tatgataaac aaggtgataa tgtattagtg gatactattt tagtaccact
    33181 tccagagaat tacgaagtaa ttaactttag tcagttgaaa attagtgatg tggtttcaca
    33241 agctattgaa gacgctgagt cttttatgag taatggtgaa tatcaacgtg cctttgacag
    33301 agttcacact gcttttcatg gatatttaat tgaaatacta aaaaaatatg aaattacagt
    33361 tcctagagac gaaaatctat ctaaattata tagtaggata caacaattaa ttgaaaaaga
    33421 aattcaacct actgaacttg ctgatatagt gaagacaacg ataaggtcat caaatggaat
    33481 gattagctct cttaatgaag caagaaatag gcattcactt gcacacccta atacaaatat
    33541 tattggaaag agagaagcaa aactaataat aggtatttca agtacagtta cagattatat
    33601 tagcggatac cttgataagt agcgtcatta actgattgct acttttttta tgactatttt
    33661 tataaaaagg atgtgataag aattggaaat gaaagtctat gtggcaaatt taggacggta
    33721 taatgaaggt gaattagtcg gagcatggtt tactccacca attgatgagg aagaaatggc
    33781 agaaagaatc ggtttaaatg aggactacga agaatatgca atacatgatt ttgagcttcc
    33841 ttttgatgtg gacgaataca cgccgattag tgaaatcaac cgtctatgtg aagccattca
    33901 agaaatcgaa ggtacaccta tttataatga actcaaagaa attcaaggca tgtggtttag
    33961 tagcttggaa gaattactgg aaaacaaaga ggatattcat tgttatagtg attgtgattc
    34021 gatggaagat gtcgcccgtt attacgtgga agaaacagga cagcttggtg aagtcccttc
    34081 caatttacaa aattatattg actaccaatc tttgggacgt gatatggaaa tagagggtaa
    34141 ttacctagtt acctctcatg gggtatttga atattgtcag taagtttaac aatccgtgtt
    34201 ataattaaat taataaatag cacggagagg tataaattat ggtaaatgta tttgaaaatc
    34261 ttaaactatt aaaactagac atgaaagata aaggctgggt gatagattcc ttttactttc
    34321 gttacaaaca gcaaaattat attgtacttg ttaagctatt tgaaaaagag gaaaaagttc
    34381 cagaatatgc tctgttaaaa ctagaatttt taaaggaaaa cgatttttca gatatgctag
    34441 ctgtctatgc taattcggta aaattatata cagacacgaa aacaataaga gaatattttg
    34501 gtatcgaata cagtagtaac ttgggagatg ttttatttca atttagccaa actcttgctc
    34561 gatttattcc gacagaagtt agtgagaaga aaaatgaaga ccaaaaagaa gccatgtgtt
    34621 tctcattaag tcaaagtgat tcagaagatc ccagaaaaaa atattgtttt tcagttagaa
    34681 gaaatccttt aaagggaaat ggtgagctgg gaaaacgaag tccttttaat gacaataaaa
    34741 cgagattgta tagacccagc ttatatgaac gattagggtc agataccaat ttaagtttca
    34801 gatattcaat gaatccagat gatgaagaaa ctgatgaagg tattattgca aaatggacaa
    34861 aaaataaaat tgaatagtta aagcattgaa agagcaatca cctattttga ttgttctttt
    34921 tctattttta cggaaaggac gtgaagacat gaaaaaaata agaagttaca ccagtatttg
    34981 gtcagtagaa aaagtgattt atgcgattaa tgatttacaa ctccctttcc caattacctt
    35041 tacgcaaatg acatggttta tcgtttcttt gtttgtcgtc atactctttg gcagtttacc
    35101 accactttct ctgattgacg gagcactact caaatactta gggattccca ttgttctcac
    35161 tgggttcatg tgccaaaaga cctttgacgg aaagaaaccc tatggatttc taaagtctat
    35221 cattacctat gctctacgcc ctaaagtaac ctacgctggc aaacaaatac gattaaaaaa
    35281 agaaacaatg aatgaatcga ttacgattgt aaggagtgaa caactagatg gcatatccga
    35341 ttaaatatat ggaagataat ctcgtattta atcatgatgg cgaagtattc gcttattatg
    35401 aattaattcc ctataattac agttttttaa gttccgatga aaaaattcaa gttcatgata
    35461 atttctgtca attgattgcc caaaatcgtg atggcaagat tcatgctctg caactagcaa
    35521 ccgaatcaag tattcgagcc acacaggaac aatcgaaaaa agaaatcact ggacgattaa
    35581 aagagatagc ttacgaaaaa atagatgaac agaccgaagc gttaatttct atgattggtg
    35641 acaatcaagt agactatcgc ttttttattg gttttaaact ctacctcaat gaacaggaag
    35701 tatccgtaaa aaatgttggg aaagaaattc aaaaagcatt tgcggaattc tttcatggtg
    35761 tgaatcacaa attaatgggc gattttgttt ctatgcctat ggcggaaatc gaacgctttt
    35821 ctaagatgga acagttgtta gaaagtaaat tatccagacg ctttcagatt cgtaaattgg
    35881 agaaagatga ttttggttat attattgaac atctttacgg gcaaacaggt gtcccttttg
    35941 atgactacga ctaccattta ccgaaacgat acgaagataa aaaagtgttg gtaaagaaat
    36001 acgatattct caaacccact cgctgtttaa tcgaagaaaa tcaacgctat ttacggattg
    36061 aacaggaaga taccactact tatgtggcgt attttacaat taatagtatt gttggtgaat
    36121 tggatttccc taatagtgaa attttttatt accagcaaca gcagtttact ttccccgttg
    36181 ataccagtat gaatgttgaa attgtcgcca ataaaaaagc cttatctacc gttcgcaata
    36241 aaaagaaaga actgaaagat ttagatagcc acgcatggga aagcgacaac gaaaccagta
    36301 ctaatgtggt ggacgcttta gaatctgtcg atgaattaga aagtaattta gaccaaacca
    36361 aagaatctat gtacaaacta agctatgtgg tacgggtatc agcaaagaat ttggacgagc
    36421 tgaaacaacg ttgtgatgaa gtaaaagatt tttatgatga tctgtctatc aaattagtac
    36481 gaccttttgg cgatatgcta ggcttgcata atgaatttat tccctcaagc aagcgatata
    36541 tcaatgacta tattcaatat gtcacgtcgg attttttggc tggtcttggt ttcggtgcta
    36601 cacaaatgat tggtgaaaat tctggtattt acgtcggtta caatctcgat acaggcagaa
    36661 atatttatct ccaacctagc cttgcaagtc aaggaattaa aggctctgta acaaatgcat
    36721 tagcgtccgc ttttattggt tcattaggtg gcggaaaatc attctctaac aatatgctgg
    36781 tctattattc agtactcttt ggcggacagg caaccattgt tgacccaaaa gctgaacgtg
    36841 gtgaatggaa agaaacctta cctgatattg cccatgaaat caatattgtt aatctcacga
    36901 gtgagaacga caataaaggg ttacttgacc cttatgtgat tttaaaactc ccgaaagatt
    36961 cagaaagcct tgccattgat attctcacgt tcttaacagg tatttccagc cgagatagcg
    37021 agaagtttcc tgtcctgcgt aaagctattc ggagtgtaac tcaaagcaaa caacgagggt
    37081 tgttattggt gattgatgaa ttgcgaaaag aagatacgcc catcagcaat agtattgctg
    37141 accatatcga aagctttgtc gattatgatt ttgcccatct gttattcagt gatggcagtg
    37201 tcacccaatc cattagcttg gaaaaacaac tcaatattat tcaagtggca gatttggtct
    37261 tacccgattc caatagctct tttgaagaat acactaccat ggaattatta agtgtggcaa
    37321 tgctgattgt cattagtacc tttgctttag actttatcca ctctgaccgt tccattttta
    37381 aaattgttga ccttgatgag gcttggagtt ttttacaagt ggcacaagga aaagctctat
    37441 ctatgcgatt ggttcgagca ggacgtgcca tgaatgcagg ggtctatttc gttactcaaa
    37501 acgcggacga tttactagat gaaaagatga agaataatat tggcttgaaa tttgcctttc
    37561 gttccactga cctagtggaa atcaaaaaga ccttagaatt ttttggtgtg gacaaggaag
    37621 atgaaaacaa tcagaaacgc ttacgtgaat tagaaaatgg acaatgttta gtttcagaca
    37681 tctatggtcg tgtaggggtt atgcagtttc acccgatttt tgaagagttg cttcatgcct
    37741 ttgataccag accgcctgtg cgaaatgagg tggaagaatg acacaaaaac aaaagaaatt
    37801 tcttcactat agttggataa cgctcctttg tgtgggcgtt attttacttt tattaggcac
    37861 gctgggcaat gcagtacaag cgacaggctt agttgatgaa accattgata caagcaatga
    37921 atactccaag tatggtttga atcaatatca gcttgattat tatgtggaca atagctgggg
    37981 ctggcttcca tggaattgga gtgatggcat aggacaatcg gtgatgtatg gattgtatgc
    38041 cattactaat tttatttgga caatttctct ttatctatcg aacgccacag gttatttggt
    38101 gaaagaagcc tatgccttag atttcattag tcaaacatcc caagcaattg gcgaaaacat
    38161 gcagacgatt gcaggcatta caccgaatgg cttttccagt tcagggtttt atgtcggttt
    38221 cctccttctt tttattctca ttgttggaat ctatgttgcc tatacaggat tggttaagcg
    38281 agaaacgacg aaagtgatac gagctgtttt aaactttgtc gtggtgttta ttctatcggc
    38341 ttcctttatt gcgtatgcac ccgattatat tgcgaaaatc aatgattttt ccaaagatgt
    38401 cagtaccgcc agtctgaata ttgggacaaa aattgttctt cctaacacgg atagtcaagg
    38461 gaaagacagt gtggacatga tacgtgacag cttattctct gttcaagtaa agcaaccatg
    38521 gctattgctt caatatggct ccactgatgt agaagcgtta ggggaagaac gtgtggaaag
    38581 tttattgtct accagtccag atacaaacaa tggtgaagat agagaaaatg tagtgattga
    38641 agaaattgaa gatcatgaca atacctattt aaccctaccg aagacgattt ccagattagg
    38701 aacagtcttc tttttgtttg tattcaatat tggtatctcg atttttgttt tccttctaac
    38761 aggaatcatg attttttcgc aagtgctatt tattattttc gctatgtttt tgccaatcag
    38821 tttcctatta agtatgttgc cgagttttga tggcatgggc aggcatgcca ttaccaagtt
    38881 atttaatgtg attatgacaa gggctggtat taccttgatt attaccgttg cctttagtat
    38941 ttccactatg ctttattcgc tgtcagcaag ttctcccttt ttcatgatta tgtttttaca
    39001 aattgtgacc tttgcaggga tttattttaa actgggcgat ttaatgagcc tattcacttt
    39061 acaaagcaac gactctcaaa acatgggcag acaagtgttt cgcagacctc gtcaaatgat
    39121 gaatcgacaa accagaagat ttcaacgcag tatgagtaag atttttcgtg gaaaagcagg
    39181 gtcaacgaag caaaagacaa tgacaacaaa gcagaatcaa accaataaag ctaaccatac
    39241 tcgtcaaaat gaacggacta caccaaagaa agaaacagca ctttccaatg tgaaaaagca
    39301 acaaggcggt atcgggcaac gattaggtga aaagacagcc catgtattgg acacgaaaaa
    39361 tcgtctagtc aataaaagaa agcaggcaaa acaacagata caggcgacac caacccatgt
    39421 caaatatgcc cttcatcaag gaaaagagaa tgtcactcat aatgtaacag actttaaaca
    39481 atctatggca gataccaaag ctcaacgcaa acaacagcgt atgaaccagc aggagcaaaa
    39541 acgaaagaac attgctcaaa agcgtttgga gctggataaa gcaaaagaaa aaagacgaaa
    39601 agaaaaccca tcatctcctg ttcagaataa ccagcagcat gttcgcaagc gtcagcaaac
    39661 cgtcacgcct gttttgtcac aaaacactaa acgtgaaaat catcagcgac cattgaaaaa
    39721 agagcaaaaa caaaagacac caccgaagca attcaatcag aggaagctaa ataaaagaac
    39781 ggataagcga ggacaacaaa catgaagcta aaaataacag tgatttgtag tgtggtcttc
    39841 tctctcttct tttttctctt attattccta gtcattttct ttgcagatga tacggacagt
    39901 ggcgaaaaca acaaggattc ttctattcca caaggcggtg tgaccgtttc accagaagtg
    39961 ttggcacacc gtccgttgat tgaaaaatat ggtaaggaat atgggattga agactatgtt
    40021 tcttatatct tagccattat gcaagtggaa tcgggcggta cagcagaaga tgttatgcaa
    40081 agttccgaat ctttgggttt accgcccaac agtttgagta ccgaagagtc catcaaacaa
    40141 ggggttaaat atttcagtga attattaacc agtgcagaac aacaaggagt ggatatagat
    40201 tctgttatcc aatcttataa ttatggcggt ggctttttaa attatgtgag gagtcatggg
    40261 aaaaaatata cctatgaatt agccgaacag ttttctaaag agaagtcggg tggtcaaaaa
    40321 gcagactatc ccaatcctat agccatacct gtgaatggtg gttggcgata taactatgga
    40381 aatcagtttt atgttcagtt ggtttcacaa tacctaaccg acacttcccc aacagaattt
    40441 gatgatgaaa ccgtgcaagt cattatggac gaagccttga aatacgaagg gtttccctat
    40501 gtctttggag gggcgtcgcc taccacctct tttgattgta gtggcttaat acaatgggtg
    40561 tatgacaaag ctggcatttc tctccctcgt gtcgcccaag atcagtatga cgctacgcaa
    40621 gaaatctcta tggaagaagc ccaagcaggc gaccttatct tctttcattc aacctataat
    40681 gcaggcacat atgtgactca tgtggcgata tatttagagg gaaatcgctt ttatcatgca
    40741 ggcgacccaa tcggttatgg cgacttaagc agtcgttatt ggcaagacca tctgattggg
    40801 gctagacgtg ttattcataa ctaagaaagg aatgaatcaa atgattataa aaatcgagcg
    40861 aaaagaaaag aaggagaaac caaagaaatc taaaaaaccg agcaagcaaa gaaaagttcc
    40921 gatgattaaa gttggcacgc ataagaaatt aacctttgtt ttatgggttt tattgattgg
    40981 tagtgtcgga tttggaatct ataaaaattt tactgccatt gacactcata ctgtccgtga
    41041 aacagaaatc attaaacagc agattgtcga taccaaccaa gtagaaagtt ttgtgaaatc
    41101 ctttgcgaaa gactactttt cgtggcaaca atcccaagaa gccattgata aacgaaatga
    41161 aaaattgacc cattatttga cggaagaatt gcaggtatta aatgaagaaa tgattcgtaa
    41221 ggatattcct actagttcta gtgtgaacga tatacaggtg tggcaggttt ctcaagtgaa
    41281 tgagaacacc tttgaggtac tgttttctgt tgaacaagtt attaccgaag acaaagacaa
    41341 ggaaactata tcgtctagct ttcatgtggt tgtccatata gatgaatcag ataatatggt
    41401 tattatcaaa aatccgacaa tgagtaagaa acctcaaaaa tcggactacc aacccaaaca
    41461 gctagaaagt gaccatacag tagatacaga aacgatggac gaaatcacta gctttttaga
    41521 aacgttcttc cagctctatc ctaccgccac agaaaaagaa ttgacttact atgtaagtaa
    41581 tcatgtgtta cctatgatta acaaggaata tgttttcgag gaattggtga atcctatctt
    41641 taccagaaaa gataaccaag taatcgtgaa tgtcgctgtg aaatatttag atcaagaaac
    41701 aaaggcaacc caaatctcgc aatttgagtt gatattagaa aaacaagata attggaagat
    41761 tgtaaaataa caaatattgg tacatgatta cagatacttt gtaatcatgt actctttttg
    41821 ataaaaaatt ggagattcct ttacaaatat gctcttacgt gctattattt aagtgactat
    41881 ttaaaaggag ttaataaata tgcggcaagg tattcttaaa taaactgtca atttgatagc
    41941 gggaacaaat aattagatgt ccttttttag gagggcttag ttttttgtac ccagtttaag
    42001 aataccttta tcatgtgatt ctaaagtatc cagagaatat ctgtatgctt tgtataccta
    42061 tggttatgca taaaaatccc agtgataaaa gtatttatca ctgggatttt tatgcccttt
    42121 tgggtttttg aatggaggaa aatcacatga aaattattaa tattggagtt ttagctcatg
    42181 ttgatgc
//
LOCUS       DACECY010000022        41494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.023, whole genome shotgun sequence.
ACCESSION   DACECY010000022 DACECY010000000
VERSION     DACECY010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.023"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1273..1938)
                     /locus_tag="IWO38_001201"
     CDS             complement(1273..1938)
                     /locus_tag="IWO38_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0313301.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..4100
                     /locus_tag="IWO38_001202"
     CDS             2064..4100
                     /locus_tag="IWO38_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ0313302.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="IWO38_001203"
     CDS             4104..4742
                     /locus_tag="IWO38_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0313303.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="IWO38_001204"
     CDS             4770..5198
                     /locus_tag="IWO38_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313304.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..6566
                     /locus_tag="IWO38_001205"
     CDS             5655..6566
                     /locus_tag="IWO38_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0313305.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            6783..7220
                     /locus_tag="IWO38_001206"
     CDS             6783..7220
                     /locus_tag="IWO38_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313306.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            7315..8199
                     /locus_tag="IWO38_001207"
     CDS             7315..8199
                     /locus_tag="IWO38_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ0313307.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            8423..11590
                     /locus_tag="IWO38_001208"
     CDS             8423..11590
                     /locus_tag="IWO38_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313308.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            11610..12155
                     /locus_tag="IWO38_001209"
     CDS             11610..12155
                     /locus_tag="IWO38_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAQ0313309.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKIQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            12183..12638
                     /locus_tag="IWO38_001210"
     CDS             12183..12638
                     /locus_tag="IWO38_001210"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAQ0313310.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            12635..13393
                     /locus_tag="IWO38_001211"
     CDS             12635..13393
                     /locus_tag="IWO38_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0313311.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            13894..15093
                     /locus_tag="IWO38_001212"
     CDS             13894..15093
                     /locus_tag="IWO38_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0313312.1"
                     /translation="MLFTSGPDRAFERLMQEKPGFDHYDGGGADAPEDCGTCRFYRPD
                     WKYQFCQFAECPYQPGKLTDYAAVKFTVKGANNAMAVFRVEKNKGYTVMSNHHLRNKN
                     LTLKAKGLLSQMLSLPEDWDYTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDA
                     KGKMADMVYTIYEQPVLENPVLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLS
                     KKEKIITDGQSTHSIPIPSPTPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEY
                     AYLIQDRSIDREQLDEIVDLMLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRF
                     VLDCLRENTTKVRNIKQYLRAMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYS
                     CGEGESL"
     gene            15115..15327
                     /locus_tag="IWO38_001213"
     CDS             15115..15327
                     /locus_tag="IWO38_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ0313313.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            15402..15878
                     /locus_tag="IWO38_001214"
     CDS             15402..15878
                     /locus_tag="IWO38_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAQ0313314.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            15875..17683
                     /locus_tag="IWO38_001215"
     CDS             15875..17683
                     /locus_tag="IWO38_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ0313315.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            17760..17975
                     /locus_tag="IWO38_001216"
     CDS             17760..17975
                     /locus_tag="IWO38_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer protein"
                     /protein_id="HAQ0313316.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            18067..18930
                     /locus_tag="IWO38_001217"
     CDS             18067..18930
                     /locus_tag="IWO38_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313317.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            18961..19503
                     /locus_tag="IWO38_001218"
     CDS             18961..19503
                     /locus_tag="IWO38_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HAQ0313318.1"
                     /translation="MFISTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVE
                     RLASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTR
                     GNAEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFAR
                     QKTPGWDVWGNEVECDLILT"
     gene            19517..19939
                     /locus_tag="IWO38_001219"
     CDS             19517..19939
                     /locus_tag="IWO38_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAQ0313319.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            19869..22268
                     /locus_tag="IWO38_001220"
     CDS             19869..22268
                     /locus_tag="IWO38_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAQ0313320.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            22300..24291
                     /locus_tag="IWO38_001221"
     CDS             22300..24291
                     /locus_tag="IWO38_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAQ0313321.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            24314..24565
                     /locus_tag="IWO38_001222"
     CDS             24314..24565
                     /locus_tag="IWO38_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAQ0313322.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            24555..25784
                     /locus_tag="IWO38_001223"
     CDS             24555..25784
                     /locus_tag="IWO38_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAQ0313323.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            25781..27862
                     /gene="topB"
                     /locus_tag="IWO38_001224"
     CDS             25781..27862
                     /gene="topB"
                     /locus_tag="IWO38_001224"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0313324.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            28011..31931
                     /locus_tag="IWO38_001225"
     CDS             28011..31931
                     /locus_tag="IWO38_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAQ0313325.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSYPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            31932..32876
                     /locus_tag="IWO38_001226"
     CDS             31932..32876
                     /locus_tag="IWO38_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313326.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            32880..33083
                     /locus_tag="IWO38_001227"
     CDS             32880..33083
                     /locus_tag="IWO38_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313327.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(33383..33619)
                     /locus_tag="IWO38_001228"
     CDS             complement(33383..33619)
                     /locus_tag="IWO38_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0313328.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(33802..34173)
                     /locus_tag="IWO38_001229"
     CDS             complement(33802..34173)
                     /locus_tag="IWO38_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313329.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(34170..35498)
                     /locus_tag="IWO38_001230"
     CDS             complement(34170..35498)
                     /locus_tag="IWO38_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ0313330.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(35459..35788)
                     /locus_tag="IWO38_001231"
     CDS             complement(35459..35788)
                     /locus_tag="IWO38_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ0313331.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(36046..36417)
                     /locus_tag="IWO38_001232"
     CDS             complement(36046..36417)
                     /locus_tag="IWO38_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0313332.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            36562..36747
                     /locus_tag="IWO38_001233"
     CDS             36562..36747
                     /locus_tag="IWO38_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0313333.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            37690..38349
                     /gene="vanR"
                     /locus_tag="IWO38_001234"
     CDS             37690..38349
                     /gene="vanR"
                     /locus_tag="IWO38_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ0313334.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDLEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFESDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            38352..39695
                     /gene="vanS"
                     /locus_tag="IWO38_001235"
     CDS             38352..39695
                     /gene="vanS"
                     /locus_tag="IWO38_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ0313335.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRNGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            39866..40672
                     /gene="vanY"
                     /locus_tag="IWO38_001236"
     CDS             39866..40672
                     /gene="vanY"
                     /locus_tag="IWO38_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAQ0313336.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            40685..41494
                     /gene="vanW"
                     /locus_tag="IWO38_001237"
                     /pseudo
     CDS             40685..41494
                     /gene="vanW"
                     /locus_tag="IWO38_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
BASE COUNT        11617 a         9542 c        10337 g         9998 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctagtac agcaagtagt aaataagcgg tataaataaa aaatatgaac tatttaaatc
     6601 aataaaactg ttcatctaaa tctttttatt agaactaaat caacagtagt ttaattacta
     6661 cttgatttag ttctttttat tttaaaaatt ttttttactt agaatccagc gaccctagat
     6721 atttttctgt ataatcttaa ataaagaaaa caatatcaaa aaatgcacgt agaagtagat
     6781 aaatggagtg gttttgcaaa tttcttttat ttctagaagt tattcacgta tacggatttg
     6841 tttttctcac aaataaaaag gcaggaagta caatggatca tttgtatata aaatatattc
     6901 atcaattttc cgttttggtg ttaggcacac taattatagc ggggattgtc gcatttatta
     6961 ttcaaggccg tgacatatta acagtacatc aagagttttt cagttatgca tttggtattc
     7021 ctctgatcgc tataaggtct gataacaaaa agtggaagaa acgctattac attctcttgc
     7081 tatttttgtg tttttatctg ccacctaaga tttttacttt attaggtata caatgcctat
     7141 cattttcttt tatttcctat atcattgttt taattttcct cagtttcata gtatggatgt
     7201 tttatcttgt caaaaaataa atgaatgttt catgaacagt acttcatcac agcctaattt
     7261 ttttctttat gctatcgtta tattaaagaa atcaatcaga agggaaagaa agtgatgaaa
     7321 aaagttttat tagtggtaaa ccccagttca ggcggtgaac aagcaaagga atttgagcaa
     7381 ttagcgatag caaaattgga atcggtgttt gatgaagtgg tcgtcttgca tacaaaaaaa
     7441 gcaggggatg caaaaaactt tactcgcgaa gcggctacgg aagggtatca tagtgtgttt
     7501 gtcatgggcg gagatggaac agtcaacgaa ggaatcagtg gaatcgctga acaagaacat
     7561 cggccaaatt tcggattttt cccattgggt acggtgaatg acttggctcg agcattaggg
     7621 ataccgctag aacctgagga agcgatcaat catttttcga ttgaatcagt caagccattg
     7681 gatattggaa aaataaatga cgactatttt atgaatgtcg tcgcaattgg ctctatccct
     7741 gaatcaatca atgatgtaga tgcggagaag aagacgaaat ttggaaaact agcttatttc
     7801 atgtcaggaa tcaaacagtt agcttccaca caatcctact cgtttcacgt agaagttgat
     7861 ggaaagaagg aagaaattga gagcagtacc ttgctgatag gactaactaa ctcagtcggc
     7921 ggttttgaaa cattacttcc aaatgcaaaa gtggatgatg gtaagctcca tttggtttat
     7981 ttaaaagatt cttctttgat cgatacgctt aaaacattgc cagacctatt aaaaggagta
     8041 gatgaatcta cgaaaaatct agcctacctt acttgtgagc aattagttgt gtcattagct
     8101 gatgaggaag aattgacgac gaatgttgat ggagatgaag gggataaatt acctgtgaca
     8161 atcaaagtat tgccctcaca tttacaggtt tattgttaaa atggaggttg tgaccgagga
     8221 gttggcggga gaacaccgct tgttcgggat aagggactgt gacaagactt ttgtcacagc
     8281 cttttttttg tacaaaaaag aaatttacac ctaatagaaa aatttttcca taaaaataca
     8341 tattttaaat atgaaagtac aattggaaat agattaaaac gataaaataa aattgtcaaa
     8401 acgtcagaga ggatggtgga gaatgaatca atgtgtaaaa aagctgattg cgtcgatttt
     8461 gtttgtcttc cctctcttga tggtgacatc cgtttttgcc gatgtagaga aaacaaacga
     8521 atcaacgcaa tcagagaatc aaagttttga caagactact ttttcagaag aatcgattac
     8581 ttctgaaact acagaatctc aaagtgaggc ttctactaat aaaaggcaag aagaaaataa
     8641 gacaagtgag acctcagaag tagctgaaca aacgtctgat agcagtgaag cggcactggc
     8701 aagagctgtt tcagcggatg ggtacagcga agctgccaca acagaagaac tggcgcagct
     8761 tctcgcagac gaatcagtaa ctaagatccg actgattcag cctttgactt tagacaggga
     8821 attagagatc aaacgagata tcgttattga ttttggtgga ttcgctcaca atttcggcac
     8881 acaccatatt tatatcaatg agatacctca gtcgatcgag ttccagaatt ttaaaggaac
     8941 agctgcacat ccaggtggat tgacaccaaa tatcgatgga aatgccatta tcattgctta
     9001 tatgaccgat ttttggggaa gcagatatca ttttacggga gaaataaaat ttaccgggac
     9061 acttgatttg tatgatgggt ccaaattagg actgatttac gctcctcgtg caactgtcac
     9121 attagatggt gtttccggtg tgttggatgt ccaaccagtg aaggaaggac tgaatgccgc
     9181 tccgggtaaa gcctattttt gtagaagcta tcaattgaat gtgatcaatg gtagtcaact
     9241 ttatggaccg tatctaggga aattctacgg ttttctagat tcaggagatg agactggtag
     9301 tggaaaaaca gcgccaggaa tccatatctt aagcggttca aaggtctctc ttgattatga
     9361 tcgggcagat ggcgttagcg atgaaggaga ggcagtcgat acgctacctg gtaatgtcat
     9421 gtttgaagtc agtggttcgg gaagcgaatt ttcggtaaat acaaagatca atattaccaa
     9481 tgacaacaat cgcggaatca tccaaatgag aggttcagga tcgaaagtca ctgtatcgaa
     9541 tggtgggaaa atcaccatca atacagcaac tacgggcgga tttcgcttgc aaggagattc
     9601 tagtcaaatc catgtatcaa gtggtggaca aatctcggtc aaaatggctg gagatgacga
     9661 tcaacccggg aacaatggga tgcgttttgt aggaaaaggc ctttctttga tcgtagaagg
     9721 agccggatca gttatcgaca tcgataagca gagtgggcgt tccagcgcag ttcgttttga
     9781 gaatggtacg caaaagctga cagtaaccaa tggaggctca ttgagggtaa gaaatacagg
     9841 tgatggcaaa tcttacgtga acagactcaa tcaaggaaat caagcgatcc aattttatga
     9901 cgcttctggt tttctagaaa gtccaggttc agctgatttt acagtcactg gagaaaaatc
     9961 aaatatcgat atacgcgcag attccggagc aactgtagat acaactggag atattgattt
    10021 aaattttacg gcaggtgaaa aaacttattt agtcatggtc gggaaaacag gggaagataa
    10081 gaccggcatc atcagtgggg atgtgttgaa tgtggactta gaatctccta cctattttga
    10141 tttccaaaac aaacggattg gaagcgagac caatggcata ggcggctggg tattccaagg
    10201 aaatggagat tcttctttaa agatcgccca ttcagaaatc gctttatggc gaaaaacagg
    10261 cgcaacattc gataactttg aaaaagaccc ggattatttt tctgatatga cagacttatc
    10321 gatcaatggg accgacttag gcaatttcgt atcaagccct agtgcaggtt taacaaatga
    10381 atttgctcag acaggtgccg gaatgaaaaa ttataatcga atcagtgcca ataatcagct
    10441 cccgatgatt gaatccttaa gagtgccaac gaatgcagat aaaaaaatct atgggcatgt
    10501 cgttgttccc gaaggtgtcg aggcaaagcc aagagatgct tggacaggcg aagtagaagt
    10561 gactttacgt attacttacg cagatacgac gaaatcatct gtagaaatta ctgctctcac
    10621 gcaaggacaa acagatgaaa atgaaggggc aggatataac ccatggggag aaggcgaaca
    10681 aggcggcttg ttcgaagttc aagtacccaa tgatgagaga ttaaaaacgg aggatcaagt
    10741 gcaagttgtt gctgctaaaa aagtaagagg tggccaagaa gtgacagaac tggactctgc
    10801 caaaacgaca gtcgatgtga ttcctcctga acctgctaat ttaagtacca cattgatcaa
    10861 tgcagcttct cgcacgatca caggtacagg tgaagcagga gccttggttt cattgaaaaa
    10921 aaatcaagct gttttgtcag aaacaactat tgatgataca ggggaatttg ttctttctat
    10981 cccagaaaat caactttttg caggagatgt attagaaatt gtattaaacg accaagcagg
    11041 agaagctact gctaagggtg tgcagaacaa gccaagtacc aacgatgaaa ttgggaatca
    11101 caatccaagt gatacgccag ttgtttatca tgatgccccg ccttttgcac ctgctacgaa
    11161 gatcgtagta gaaggtggtc tctcatttct tgccccttcc aaactcgatt ttggtcagat
    11221 caaagcaact ggtttgacac aagaagcatt tggccaaatg gatatacagg aaaaattatg
    11281 gatttatgat cagcgagaaa cgaaaaatcc gtggatctta acgataaaat tatctcagcc
    11341 tttcacaaat gatgcaggat tttctatgca ggatgtccta tatttcaaga aaggagagga
    11401 gtatcaatta gtgaatgaag aagaactgga aattgtcaga ggacaaaatt caacgaatgt
    11461 gcaagcagat tattcagaat atttaaataa cggtcgggcg tttaaacttg tcttaaccaa
    11521 agaaaatcag atagctggta attactcagc agagataaca tggacattag cagatgcgcc
    11581 gacagaataa agatcaagga ggatgattga tgaatcgaat atggaataaa agtcatcgaa
    11641 acaactgctc tttacttatg atccttctcc ttggattcat cctttggcag acacctgtcc
    11701 atgcctccga attcaatttt gcggtagatt ttgaattacc ggctaatcag attgataaga
    11761 acaaaacata tataaatgta aggatggagc ctgggaaaat acaggaaatc ccctacacgg
    11821 tctttaaccg aacagataaa tcagtaacag ttgagacatt gatccactcc gctacgacga
    11881 attcaaacgg tgtgatcgaa tatggagaat caaaaagtaa atttgatgaa acattaagtt
    11941 atgatatgaa aaacttagca aaaacagtgg gagaaataac tgttcctcct caaagtgcgg
    12001 tcactgagac actttcactg aaagcgccag aaaaagagct ggctggtgtg gtcgcaggag
    12061 ggatcacgtt tcaagaaaaa aaagagaacg tttctaacaa aagtacagtt ggtgtcgtca
    12121 atcagttttc tcaagtgaaa gcaatcttga tttgaaatag cgaaaaagag tcttccccag
    12181 atttgaaatt aaaaaaagct tatgcggatc aaagcgatgg aagaaatgtg attaaggcga
    12241 atcttcaaaa tccaaatgct tcttatatgt tcggcttgac tacatcggtt gaaatctaca
    12301 aaaaagaaac aaaagagcct tacctttcaa taaaaaaaga aaaaatagat gtagcaccca
    12361 atacaaattt tgatttactt gtcccattag aaggaaaaaa attagctccg ggacattatc
    12421 aagcggttgt gaagggtgct tggaaagaaa aacgctggaa tctccatacc gattttgaaa
    12481 taactgatga tgcagcggac caattgaatg aaaaggatat tgatttaaaa aatcagccag
    12541 aaacatttga ctggcgatgg ctgatacttg ttggagtcat tcttattggc ttatttgctg
    12601 gattatttat tttaaaagaa agaaggaaaa agaaatgaaa aaaacaattg cattaagtac
    12661 cgttctagct ggaagtctat tattatgtac acaaagtgtt tttgctgaga ataagagctc
    12721 agccactaca gaaggaacga tttctgtgac caaaccagat gctgcaaatc ctgaagacgt
    12781 tgttccagtt cctcctgttg atccaaatga tcctgatact ccttttacac ctgacgaacc
    12841 aaatcaggga acatccggat tactgacgat tgaccaagct ccagatcttg attttggaac
    12901 aatcaagtta ggtacagcaa aaacagtata tgctgcatta caaagcggaa cggacagtac
    12961 tggagcacaa acacaagtac agaatttcgt acaagtaacg gataaaagcg gaaattatgc
    13021 cggttggaaa ttatcagtca aacgaacaga atttattggt acaacatctg atacgaataa
    13081 actaactggt tcaaaggtga aattcaaaaa cgccgtgatt cgaaatagcg caactaatag
    13141 cagtggaatg ccatctaccg tttctggaac tggcgctgaa ggaatcgaaa tcccagcaga
    13201 tcaggcagta gatctagtaa ctgcaggagc aaatgaaggg attggcacat ggatttattc
    13261 attaggagag aatgtggaac aagggagaca atcagtggaa ttatttgttc ctgtatccaa
    13321 ttatgctgta gacaactacg tttcaacatt cacatggtcg ttaacagatg ctcctacaga
    13381 agaggaagcg taaaatcata tcaacgattt cagggagtgg agccgattca aggcccactc
    13441 cctgaaatct gctatgctta cgtctcagct cttattcatc aactcgaagc agttttttgg
    13501 aagtcatgga aacgttcctt tgtcttttca aagatctttc gaccaacgag taaggagtca
    13561 ccgttgaaaa attctaacac ataatgagca gaatcaattt tctgcaattg cgataagttg
    13621 atacagtaag attttaaaag taaaaatttt aggttatcgc tgaaagcccc ggaaatacgg
    13681 gcatttcgca ggatatgaga actcaattta ctaccaattt actactttcg gattgagccg
    13741 catatatgct gaaccatact gctaataccc gccaccccaa atacggcaca tcataaattg
    13801 aatacgacac cgtaaacgcg gcgttcctct ttcccgtcat gggagaacag gacgccgctt
    13861 ttttatgccc aaatagagaa aggaggcgac gccatgctat ttacttccgg ccctgaccgg
    13921 gcgtttgaac ggctgatgca ggaaaagccc ggtttcgatc actacgacgg cggcggggcg
    13981 gacgcgccgg aggattgcgg cacctgccga ttttaccgcc cggattggaa atatcaattc
    14041 tgccagtttg cagagtgtcc ctatcagccg ggcaagctga ccgactatgc agcggtcaaa
    14101 ttcacagtga aaggagcgaa caacgctatg gcagttttcc gtgtggaaaa gaacaaaggc
    14161 tatacggtga tgtcaaatca ccacctgcgg aataagaacc tgaccttaaa ggccaagggc
    14221 ctgctgtcgc agatgttgtc cttgccggag gattgggact ataccctaaa aggactgtcc
    14281 tatatcaacc gggaaagcat cgacgcgatc aggacagcgg tatgggagct tgaaaaagct
    14341 ggatatatcc ggcgtgagca gggccgtgac gcaaaaggca aaatggccga tatggtctat
    14401 accatctacg aacagccggt attggaaaac ccggtattgg aaaatccaac atcggataac
    14461 ccggtgttgg aaaatccgac atcggataat ccggcgtcgg aaaatccaac gcaattaaat
    14521 aaagaagtac aaagaactga cttatcaaaa aaagaaaaaa taattacaga tggacaaagt
    14581 acccattcca ttcctatccc atccccgacc cctgccccta tggagggcga ggcagcggct
    14641 acgccgccgg aacggaaaag gacaggaaag gatgaggctg tacggattta tcgggaaatc
    14701 attttggaga acatcgagta tgcctatctc attcaggaca ggagcattga ccgtgaacag
    14761 cttgacgaaa tcgtagacct gatgctggaa accgtctgca ccgcccgaaa gacaatccgt
    14821 gtcgccgggg acgactaccc tgccgagctg gtgaaatcca agtacatgaa gctgaccggc
    14881 gaacatatcc gctttgtcct tgactgtctg cgggagaaca caacgaaagt ccgcaatatc
    14941 aaacaatacc tgcgggctat gctgttcaac gcccccagca ccatttccaa ctactacacg
    15001 gcccttgtcg cgcacgacat ggcacagccc gattggggaa agcctaaatc cggcctgccg
    15061 gactattcct gcggcgaggg cgaaagcctg taacgagaaa aggaggacga ccctatgaag
    15121 cagggcgcat tgatttttga cgagcgaaca gaccgttacg acatccgctt tgatctggcc
    15181 gactattacg gcggcctgca ttgcggggag tgtttcgacg tgatgatcgg cggcaaatgg
    15241 aggcccaccc gcattgaaat ggccgaaaac tggtatcttg tcggcatccg ggccgacgac
    15301 ctttccggcc tgcgggtgcg gatttgacct atatctacct gcggcgtctg gtggttccgc
    15361 cttagacgcc gcttttgact ttctgacaag gagggatgcg attgcaggaa gaagttactc
    15421 aaaagaccat tgccctgtcc atgaaaacgg gcaagctcac ggctcaagtg ctgcaagcgg
    15481 cgctgaaaaa gtacctgcaa caccgggcca agggcaaaaa cacgctgcac cacggccagc
    15541 agagcctaaa gcagcttaaa aagcatgggg ctgatctctc caacattgag atcaccgagg
    15601 ccaacattgg agcgttcaag ccatgcgcca agaaatacgg cgtggatttt accttacgaa
    15661 aagataagac cacccagccg ccccactacg ttgtgatttt caaggccaag gatgcagata
    15721 accttgatct ggcgtttcgg gagtttaccg ccaagacgct ttccaaggag cagcggccct
    15781 ctatccgcaa ggttttgtcc gctgtgaagc aaaaggcggc ggcccagacc aagcagcggg
    15841 ccaaggagaa aatcaaggaa agggggctgg aactgtgaag cctgacgtaa agaaattgct
    15901 gatcctgaac ctcccgtatc tgatttttgt gtacctgttt gcgaaatgcg gggaggcgta
    15961 ccggctggcc gctggtgctg acctgtcggg gaagctcctg cattttgcgg atggttttgc
    16021 ggcggctttt gccaacccgc tgccaagcct gcacctgttt gacctgtgta tcggtatcgc
    16081 cggtgccgtg atcgtgcggc tggtggtgta ctgcaagagc aagaacgtga agaaataccg
    16141 caaaggcatg gagtatggca gcgcccgctg ggggacggcc aaagacatag cgccttacat
    16201 cgaccccaag tttgaaaata atatcctgct cacccaaacg gaacggctta ccatgaccgg
    16261 caggcccaaa gaccccaaga cagcgcggaa taaaaacgtg ctggtgatcg gtggcagcgg
    16321 ttcagggaaa acgagatttt tcgtgaaacc caacctgatg caatgctttc cgaccacgga
    16381 ctatccgact tcgtttgtgg tgacagatcc gaaaggcact ttggttctgg aaaccggcag
    16441 gatgttccag caggcgggct accgcattaa aatcctgaat accattaact tttccaagtc
    16501 catgaagtac aacccctttg tctacatcca ttcggaaaag gacgtgctaa agctggtaaa
    16561 taccctgatt tccaacacca agggcgaggg ggaaaagtca gcggaggatt tttgggtgaa
    16621 gtcggaacgg ttgttttatt cggccctgat cggctatatc tggtacgagg ccccagcgga
    16681 ggaaatgaac ttctcaacgc tgctggaaat gataaacgcc agtgaagccc gcgaggacga
    16741 cccggagttt caaagccccg tcgatctcat gtttgagcgt ttggagcaga aagaccccga
    16801 ccattttgcg gtgcgccagt acaagaaatt cctgctgtcc gcaggcaaga cccgttcttc
    16861 catcctgatt tcatgcggtg ccagacttgc gccctttgac atccgggagg tacgcgagct
    16921 gatggaggac gacgaaatgg agctggacac catcggggac gaaaagacgg tgctgtatct
    16981 cattatgagc gatacggaca cgacctttaa tttcattctg gctatgctcc aaagccagct
    17041 aatcaacctg ctttgtgacc gggctgatga tgtgtacggc gggcggctgc ctgtccatgt
    17101 acgcctgatt ttggatgaat ttgccaacat tggccaaata cccaatttcg ataaactcat
    17161 tgccaccatt cgaagccgtg aaatatcggc ctctattatt ttgcagagcc agtcgcagtt
    17221 aaaagcgatt tacaaggacg ccgccgagat catttcggat aactgcgata gtgtgctttt
    17281 ccttagtggg cgcggcaaaa atgccaagga gatttccgat gcgctgggga aagaaacgat
    17341 tgacagtttc aacaccagcg agaaccgggg aagccaaacg tcccacggat tgaactatca
    17401 aaaattagga aaggagttga tgtcccaaga cgaaattgcc gtgatggacg gcggcaagtg
    17461 tatcttgcag ctacggggtg tgcgcccgtt tttatcggag aaattcgata ttaccaagca
    17521 tccccggtac aaataccttg ccgacgccga caagaagaat acctttgacg tggacagatt
    17581 tttgactatc acgcgccgga aacggcagca agtggtaacg caggatgaag tattcgacct
    17641 gtatgaaatc gacctgtcgg acgaggacgc cgccgctgaa taaacagcgg cttttttcat
    17701 tccaacaaca aaatcaaaaa atcattatga gccggaaacc ggcagaaaga ggtaaattta
    17761 tggcattttt taattccgca gttactactt tgcagactat cgtgatcgga ctgggcggcg
    17821 ctctgtgcgt atggggcggc atcaatctgt tggagggata cgggcaggac aatccggggg
    17881 caaaatcgca gggtgtcaag cagcttgtcg ctggcggcgg cgtggccctg atcggtgtca
    17941 ctctggttcc cctgctgtcc gggctgctgg gctgatcctg gcctttgtcc ctgtccaaac
    18001 ccttaactaa tcgcctccgc gtcctcccgg ccagaccggg agggcgcggg aaaggaggtg
    18061 ctacccgtga ttagtgaaat cattgaagaa tggataaaag gcatcctcat ggacggtatc
    18121 acgggcaacc tgtccgggct gttcgatacg gtaaatgcca aagtcggaga aatcgccgcc
    18181 gacgtgggac gaaccccgca ggcatggaac agcggtattt tcaatatgct gcacagcctg
    18241 tcggaaaccg tgatcgtccc catagccggg gccatactcg cccttgtcat gtgctatgag
    18301 cttatccaga tgataacgga aaagaacaat atgcacgact ttgacagctc catgttcttc
    18361 cgctggatat tcaaatccgc gtttgcaatc ctgatcgtaa cgaacacatg gaacatcgtc
    18421 atgggcgtat tcgacgccac ccaaagcgtc gtcaatcaaa gcgccggtgt gattatcggg
    18481 cagacatcca ttgattttaa caccctgata ccagaccttg aaagccaatt ggaggctatg
    18541 agcgtgggcg ctcttttggg gctgtggttc caaacccttg ttgtgggcct caccatgaat
    18601 atcctttcca tctgcatttt ccttgtgacc tatggccgca tggtggaaat ctatgttgtc
    18661 acgtcgctgg ggcctatccc ccttgccaca atgggcaacg cggaatggcg cggcatgggc
    18721 caagggtatc tgaaatccct gctggcgctg ggcttccaag cctttttaat catggttgtg
    18781 gtgggtatct acgcggtgct gatacagaat atcggttccg cttccgacat atccggcgca
    18841 atctggggct gtatgggcta tacggtgctg ctgtgcttct gcctgttcaa gaccggcagt
    18901 atatcaaagg ccgtattcgg ggcgcattga tcccaaaatc caagcataca caggaggaac
    18961 ttgtttattt caacgtacaa catcatttac gctgacccgc cgtggcggta tgactgcaaa
    19021 aacgtacagg gcgcagcgga aaatcactat tccaccatga gcattgacga gctgtgcgcc
    19081 ctgccggtgg aacggctggc gtccaaagac tgcctgctgt ttttgtgggc cacattcccc
    19141 atgctgccgg aagctctgcg gttaatcaaa gcgtggggct tttcattcaa gaccgtggct
    19201 tttgtatggc tgaaacagaa ccgtaaaagc cttacatggt tttacgggct gggccgctgg
    19261 acaaggggaa acgcggaaat ctgcctgctg gcaacacacg gcaagccgaa gcgtcgttct
    19321 gcctctgtcc atcagtttat tatcagcccg attgaacaac acagcaaaaa gccggatgtg
    19381 acccgtgaaa aaatcgtaga gctggcgggc gatttgcccc gcgtggagct gtttgccaga
    19441 cagaaaaccc ccggctggga cgtgtggggg aacgaggtgg aatgtgacct tatcctgacg
    19501 tgaaaggagg tgccaaatgg cttatgtaac cgtccccaag gacttaaccc atgtgaaaag
    19561 caaggttgtg tttgggctta ccaaacggca actgatctgt ttttccgggg cgctggtgat
    19621 cggcgtcccc ttatattttc tcacccgtga ctatcttacc aacagcgcgg cggccctgct
    19681 catggtattt gccatgctgc cgggcttttt gcttgccatg tacgagcgtc acggccagcc
    19741 ccttgaagtg gtgattatgc agatcatcca gtgctgcttc atccagccaa aggagcgtcc
    19801 ctatcagacc gacaacacct acaccgccct tgtgcggcaa taccagatgg aacaggaggt
    19861 aaaggccatt gtccaaaaag gaaatcccaa gagaaaccga aaaaacaaag ctgacccgcg
    19921 cccagcgaaa agaaattgac gcggtaatcc gtaaatataa gggcgacggc aggccccaca
    19981 cggcgcagca gagcatcccc tacgaggtca tgtacccgga cggcgtgtgc cgggtatcgc
    20041 ccggcgtctt ttccaagtgc attgagtttg cggacatcag ctatcagctt gcccagccgg
    20101 acacgcagac ggctatcttt gaaaaactgt gcgacctgta taactatgtg gacgcctcca
    20161 ttcatatcca gttttctttc ctcaatcgga aagttgaccc ggtgcagtac gccaagagct
    20221 ttgagatcgc gccgcagggg gacgacttcg acgacatccg ggccgagtac accggcattc
    20281 tgcaaaagca gcttgcaaac ggcaacaacg gcatggtaaa gaccaaatac ctgacattca
    20341 ccatcgaggc cgaaagcgtg aaagcggcgc gagccagatt gaagcgcatc ggctttgacc
    20401 tgttaggcta tttcaagagc atgggggccg tagcccatgt gatggacggc tgggaacggc
    20461 tgaacctgct gcatggcgtg taccacccgg acggcgagat attcaatttt gactggaagt
    20521 ggctggcccc gtccggcctc tccaccaagg attttattgc cccgtcctcc ctgtgcttcg
    20581 gcaatgcaaa gacattcggc atgggcggca aatacggcgc ggtaagtttc ctgcaaatcc
    20641 tgtccccgga gctgtcggac gatatgctgg ccgacttcct caatacggaa agcggtgttt
    20701 tagtcaatct ccatgtgcag gccattgaac agaccaaggc gatcaagacc atcaagcgca
    20761 aaattaccga ccttgacgct atgaagattg cggagcagaa aaaggccgtc cgcagcggtt
    20821 acgatatgga cattcttccc agcgaccttg ccacctacgg cgaggacgcc aagaagctgc
    20881 tgacaaagtt gcagacccgc aatgaacggc tgttccagtt gacgttcctt gtcctcaacg
    20941 tggccgacac caagcaaaag ctgaataacg acgtgttcca agccgcaggc gtggcccaaa
    21001 agcataactg tccccttgtc cggctggact accagcagga acagggcctt gcaagcagcc
    21061 tgccgctggg cgtcaatcag atcaagatac agcgcagcct tacgacttcc agcgtggccg
    21121 tgttcgtccc ctttgtgacg caggagcttt ttcagggcgg cgcggccatg tattacggca
    21181 tcaacgccaa gtcccgcaac atgattatgc tggacaggaa gcaggcccgt tgccccaatg
    21241 ccttgaagct cggcaccccc ggcagcggca aatccatgag ctgtaaatcc gagatcgtca
    21301 gtgtgttctt aacgacgcct gacgatattt ttatttcaga cccggaggcc gaatattacc
    21361 cccttgtcaa acggctccac ggacaggtaa tccggctgtc gcctaccagc aaggactttg
    21421 tgaacccgct ggacatcaac ctgaattatt cggaggacga taacccgctg gccctgaaat
    21481 ccgactttgt gctgtcgttc tgtgagcttg tcatgggcgg caagaacggg ctggaagcca
    21541 ttgaaaagac cgtgatagac cgggctgtgc gcgtgatcta ccggccctat ctggcagacc
    21601 cccgcccgga gaatatgccg atcctgtccg acctccacaa agcgttgtta gatcagcatg
    21661 tcccggaggc tgaccgggtg gcgcaggccc ttgacctgta tgtgtccggc tccctgaacg
    21721 tatttaacca ccgtaccaac gtggacatcg gcaaccggct tgtgtccttt gacattaagg
    21781 agctgggcaa gcagctcaaa aagctgggga tgctcattgt ccaagaccag atatgggggc
    21841 gcgtcacggc caaccgaagc caaggaaagg ccacatggta ttttgccgac gagttccatt
    21901 tgcttttgaa agaggaacag acggcggcct attctgcgga gatttggaag cggtttagga
    21961 aatggggcgg cattccaaca ggtgccaccc agaacgtcaa agacctgctt tcctccccgg
    22021 aaattgaaaa tatccttgaa aacagcgact tcattacgct gctcaatcag gcgtccgggg
    22081 atagaaaaat ccttgcggaa cgcttgaatt tgtccaccga acagcagaag tacatcgaca
    22141 attccgaacc cggcgagggc cttttgatct ttgaaaacgt ggtgctgccc tttaccaacc
    22201 ccatcccgca caacacccag ctttacaaga ttatgacgac ccgcttaaac gaggtggcgg
    22261 gagtatgacc cggcgacgcc acaaaggagg tgaaccacca tgaaagagct gaaagccaag
    22321 gcgaaagtga cgcaggccat gacccgcgac gggctgacgc tggaaaatca ggccaccggc
    22381 gaggtggaga acatttccag ccgggaggcc gaacaggatt tgtccgcgcc ggggccgggc
    22441 ggcaccgccg ataagctgct ggaacgggcc gacgccatcc atgacggcca taaggccaag
    22501 aaagccgcga agcaggccgg ggctgtggcc gccgaggggg acgctgccct gcaccggcca
    22561 tccgcccggc tccaactgtc ggaggaagaa cgggccgacc cggatttaca gccctatatc
    22621 cgaaaatcgg acaaacgggc cgataagctg gataaggccc gcgccgccat ccccaagaag
    22681 cgcgtcccgg tcaaggaaac cgtctttgac gaggcaagcg gcaaagcaaa gaccgtcctg
    22741 cactttgaca aacaggacaa ggggcctccc tccctgaaac ccaatccggc aggccgtcct
    22801 gtttccgagg tttttctatt cggccacggg aaaatccatg aagtcgagca tgaaaacgtg
    22861 ggtgtggagg gcggccataa gggggaggaa cttgccgagc ggcaggccgg aaaagccatc
    22921 cgcagcacca tccggcacca caggctgaaa ccctactggg acgcggaaaa ggccgagcgc
    22981 aggctattgt ctgccaacgc ggagtatttc taccagaaat ctttgcggga taacccggag
    23041 atcgcacagg cggccagcaa ccccatttcc cggttctggc agaaacagca gatcaagcgc
    23101 cagtacgcca aggccgcccg gacagcgggc cagaccgcag gcgcggcggc acagggcgcg
    23161 gccaagacag ccggggcctc cacgaaagcg gccaaggccg cagccaaaga aagtgaacgg
    23221 gcggcctcct ttgtggcccg ccactggaaa ggggcgctgg ccgtccttgt gttggggctt
    23281 ttgatgctgc ttatcatcgg cggcttacag tcctgcaccg ccatgttcgg cagcgccgga
    23341 agcggcatca cggcgtcctc ttacttttcc gaggatagcg atatgttggc cgccgaggac
    23401 gcctacgcgg agctggaagc cgagctgcaa gactatctgg acaactacga ggccacccat
    23461 gactatgacg aataccattt tgaccttgac gagatcagcc atgaccccta tgtgctgatt
    23521 tccattctgt cggcgctcca tgacgggata tttaccatcg gggaggtaca gggtgatttg
    23581 tccatgctct ttgacaagca atatatcctg acagaaaacg tcgtggtaga aacccgttac
    23641 cgcaccgaaa ccgatacatg gacggacgcg gagggcaaca cccatacaga cacctaccaa
    23701 gtgccgtatg actattacat ctgctacgtc acgctggaaa acttcgacct gtcccatgtg
    23761 ccagtctata tcatggacga ggaaacgcta agtttctatt ccctgtatat gtccacgcta
    23821 ggaaaccggc ctgatctgtt ttccggcagc cagtacccca acgcctccac cctgacgccg
    23881 ccgacctact acgacgtacc cccggaggct ttggaggatg ccacctttgc cgccatgctc
    23941 aaagaggcgg aaaaatatat cggttatccc tatgtgtggg gcggcagctc tcccagcacc
    24001 agctttgatt gcagcggctt catttcgtgg gtaatcaatc atagtggatg gaacgtggga
    24061 cggctggggg cgcagggcct atataacatc tgcacccctg tttccagttc gcaggccaag
    24121 cccggcgacc ttgtattttt caagggaacc tacgacaccc ccggcgtgtc ccacgtcggc
    24181 ctgtatgtgg gcaacaatgt gatgctgcat tgcgggaacc cgatttccta cacgaacctt
    24241 acttcaaact actggcaatc ccatttttac agttacgggc gtttacctta acgccagaaa
    24301 ggagctttca attatggcaa ccaaacttga ccgcatcgaa aaggacatcc agaaaaccaa
    24361 gtctaagatc gcggagtttc agaaacagct tcgggagctg gaaacgcaga aaaccgaaca
    24421 ggagaatttg cagatcatcc agcttgtgcg cggcatgaac atgaagccgg aggaatttgc
    24481 cgcgttctta cgcagcggcg caatgcaggc cgcccctgcc gcaacccctt accatgaaca
    24541 ggaggacaat gcccatgaag aataagacca gacggatttc ccgtattctc cccgcacttc
    24601 tggccgtgat gctctgcgtc acggcctttc ccgttaccgc ccttgcaggc ggcaatgacc
    24661 cggcaccggc cccgctgccg gaggctacaa ccgaacccgc tacgggcggt ttggagcctg
    24721 aaacagacga aaccggcacc gccacggacg ggacagaaac cgaggccgaa aaagataccg
    24781 gccctctgac cggcaaggac atcaccgacc tgttttccgc gctgttcggt tccaaggtga
    24841 gcattgccgc cacggacgac ggcatccaga tcaccaccgg caccgacaaa aaagaacctg
    24901 aacagaccgg caccgtcacc accaacggcg gcaggctgaa tgtccgcacc ggcgcggggc
    24961 ttgacaagac cgcgttcacc cagcttccca atgggacgac ggttgaggta gtcggcacag
    25021 acggcgactg gattaaaatt ctcttgccgg agcgcatcgg ctatgtccat tccgactata
    25081 tgaccgtcag cgaaaaggaa gtagcggcta ccggcgaggg cggtttttcc ctctccattg
    25141 acccggagga aattgcttct ctgctggaac tgttcaatgg cggcggggcc agcgccgccc
    25201 tgtccccgga cgggaacctg tcactgattg acgacatcgg cagcgccgcg aaaaatggca
    25261 agcagtttat caccgtggaa accaaaaacg gcaatgtgtt ttatctgatt attgaccggg
    25321 acgacgaggg aaaagaaact gtccatttct taaatcaggt ggatgaagcc gatctgctga
    25381 cgctgatgga ggacggggaa accgtcaccc ccgctgctgt ctgctcctgt accacgaaat
    25441 gcaaggccgg ggctgtgaac acgaattgcc cggtatgtaa aaccaacctg accgagtgca
    25501 gcggcccgga gccgcaggaa ccccagccgg aggaacccga agccccggag gaagaaccca
    25561 agggcggcgc gggcggcctg atcgtgttcc tgctggtggc gctggcgggc ggcggtgcgg
    25621 ccctttacta tttcaagttc aaaaagccca aggctgaaac caccggcagc gacgatttgg
    25681 gcgagtatga ttttggggag gacgaggatt tggaggacga ggaacccgaa accgagtttg
    25741 acccggaagc tgattttgtg agtgaacagg aggacgagga atgacatata aacttgtaat
    25801 cgcggaaaaa cccagcgtgg cccgcagcat tgcgggcgtg atcggggcca cggagaaaca
    25861 cgacggctat ttggagggga acggctatct ggtgagctgg tgcatcggac atctggtgtc
    25921 ctttgccgac gccggacgct atgacgagcg tttcaaaaaa tggcgctatg aggatttgcc
    25981 cattctcccg gaaacatggc agtacatcgt ccccgacgac aagaaacagc agtttgacgt
    26041 tctctgttcg ctgatgcagc gccccgacgt gaccggcctt gtgtgcgcca ccgacgccgg
    26101 gcgcgagggg gaattgattt tccgtttcgt ctaccagatg gcgggatgcc aaaagccctt
    26161 tgagcgcctt tggatttcct ctatggagga cagtgctatt aaggacggct ttgcccacct
    26221 gaaaccgggc ggcgactatg atccgctgta tcaatcggcc ctgtgccggg ccaaggccga
    26281 ctggctgatc ggcattaacg ccacccggct tttttccgtc ctgtaccaca agaacctgac
    26341 tgtgggccgc gtccagacgc cgaccttaaa aatgctggtg gacagggacg ccaagattac
    26401 cgatttcaag aaagagaaat atcacatcgt ccatatcacg gcgggcggcg cggacgcggt
    26461 aagcagccgc ttttccgacg ctgccgaggc aaacaccgtc aaggcggctt gtgcaaaagc
    26521 gcaggccgtt tgcgtttccg tgacgcggga gaaaaagacg gaacagccgc ccaagctcta
    26581 tgaccttacc accttgcagc gggaggctaa ccggcttttc ggctataccg ccaagcagac
    26641 ccttgactat gcccaggcac tgtatctaaa gaagctgctg acctatccga gaacggatag
    26701 tcaatatctt accgacgata tgctgcccgc cgtggaaagc cttgtgtccg gcctgtggga
    26761 gctggtgccg tttgccaagg gcctgaacct ttccccgcag tttgaccgca tcctgaacag
    26821 caaaaaggtg tccgaccacc acgccattat ccccaccgct gaatttgtga agcagggctt
    26881 tggcactctg gccgagagtg aaaaaaagct gctgtccctg atctgctgca agctcttatg
    26941 tgccgtggcc gggccgcata tctatgaggc cgtcaccgcc acatttacct gtgccgggca
    27001 ggagtttacc gccaagggca agaccgtcct ttcccccggc tggaaagaga ttgaacgccg
    27061 gttccgttct tccttaaaga ccgacgccga cgaggacgcc gaggcggtgc gggagctgcc
    27121 ggagctttgc gagggccaga cctttgcaga cgtggccgcc tctgtcaccg agcatttcac
    27181 cacgccgccc aagccctaca ccgaagatac gcttttgtcc gctatggaac gggccggggc
    27241 cgaggatatg ccggaggatg ccgagcgcaa gggattaggc acaccggcca cccgcgccgc
    27301 cattctggaa aagctggtgc agatgggctt tgtccagaga aaaggcaagc agctcattcc
    27361 caccaaggac ggcatcaatc tggctgtggt gctgccggag gcgcttactt ctccccagct
    27421 tactgccgaa tgggaaaacc ggctgaccga gatcgccaag gggcaggccg atcctgacga
    27481 atttatggac ggcatcgaga cgcaggcgcg ggagctggtg cagacctatt cctgtatcag
    27541 tgaggaaaag cagaaattgt ttcaggccga gcgtgttcct atcgggacgt gtccccgctg
    27601 tggtgaggct gtctatgagg gcaagaaaaa ctactactgc ggaaaccggg cctgccagtt
    27661 cgtcatgtgg aagaatgacc gattttttga ggaacgcaag aaagcgttca ccccgaagat
    27721 cgccgctgcg ctgctcaaaa ccggcaaagt caaggtaaag ggcctctatt ccgtaaagac
    27781 cggcaaaacc tacgacggga cggtgctttt ggctgatacc gggggcaagt acgtcaacta
    27841 ccgcattgaa cacaggagct aaaggacagt aacacagcaa gcatagggag tgtggctaca
    27901 ctcccggtaa aatcccccgg ccgcgtccct gccgggccgg ggatttttgc ttgttgggga
    27961 agtccccaac cccctatttt ttgaaacaga acagagaaag gagtgaccgc gtgaacgaag
    28021 tattttctat ccaagtccat aaccggcagt ttttggaaca gggcaaacaa ggcgtctggc
    28081 tggatttgcc caccacaacc gaaacgctgc aagccgccct gcgggatgtt ggcattactg
    28141 ccgacaatcc gcaggatttt tttatcaacg ggtattccta cccggaggac agacaccttg
    28201 ccattcccta tgacatggtg ctggccgccg acgtggacga gctgaatttc cttgccgccc
    28261 ggctgggcca gcttgacgcc gccgagatcg cagagctgaa cgccgccctg caaaatcccc
    28321 gtggcggctt tgagaatatc ggccagatca tcgactacac cgaaaatgtg gattactatg
    28381 tgcatcttcc cgacgttagg ggggatggac agttgggcga ctactacctg aaccgttccg
    28441 gcatggtgga tatgccggag gaatggaaag ccgggattga tacggcgcag tttggacgcc
    28501 acattgccca gcaggaacaa ggcgttttta cccagtacgg ctatctggtg agaagcggcg
    28561 acgaatggca gcgcgtccat gagggccagc cggtgccgga ggaataccgg gtgatgcact
    28621 acccccagcc ggaagctgac cgggaggaac cgcaggccca gcagccggag gcatccgaaa
    28681 gccccgacaa agcgccgccc ccggttatcc cgatcatgct ggacggcatg aacagcaaag
    28741 aacgcatgaa agtgattact gaccgtctgg aaaccggcgt acaggagctt tttgaaagta
    28801 gccgctatat ggcttactta tccgctatgt ccaagatgca cgattacagt ctgaacaata
    28861 cgctgctgat cgccatgcag ggcggttcga ttgtggcggg ctataacagg tggcaggatg
    28921 aatttgaccg ccatgtgaag cggggcgaga aaggcattaa gatactggcc ccggccccgt
    28981 tcaaggttaa gcagcagatg gagaaaatcg acccggccac ccagcagccc gtgatcggcg
    29041 cggacggcaa gcccgtgacc gaagaacagg aagtgacggt tccggccttt aaggtggtga
    29101 cagtctttga catcagccag accgagggca aggattttcc catgccctat atcagtgaac
    29161 ttaccggcga cgtgcagcag tatcaggatt tctttgccgc gctggaacgg acatcaccct
    29221 atcccataag cattgagccg atttcagacg gggccaaggg ccgctgcttc tacgcaaaac
    29281 agcgcattgc catcaacgag ggcatgagcg aattgcagac cctaaagacc gccatccatg
    29341 agattgccca tgccaagctc cacgccattg accgggaggc ccccgccgag gaacaggcgg
    29401 cccggccaga ccgcagcacc cgcgaggtac aggccgaaag cgtggcctat accgtctgcc
    29461 agtattacgg gctggacacg tcggactatt cttttggcta tgtggccgat tggagcagcg
    29521 gcagggagtt atccgagctg aaagcctccc ttgaaaccat ccgggccacg gccagcgaat
    29581 tgataaccga gatcgacggc cattttgccc agcttcagca ggagcgcgag gcccggcagg
    29641 aacaggaggc cgtcacggtt gacgttaccc cggaggcagc ggccccggcg caggccgagg
    29701 aagaacaggc cccggccctt gatccggcag ccgagccgct tgtgactgtg ctatggagcg
    29761 aaagccccca gctaaaggac ggccagcaaa tgccgttgca tgaggccgac gcgctgtttg
    29821 cccgtctgga tgcagcatat ccggcagagg caggctatga caaaaccgcg ttccgcattg
    29881 actgtatctt tcagggcgag ccggacagct acgaggggcg gcaggatttt ggagataggg
    29941 acggttccct gatcgaacat atccagagct accaccaatt ttacgcgcag gacgaaaact
    30001 ggaaaaagca tgtaatcgct acaaccggca tagagggctg ggagcaggac aaggcccatc
    30061 gggatatgct gttaggcgag tttgtcccct atatgaagct gcattgcagc cttgcccaaa
    30121 tggaacagac ggcgcagggc cgcttacagt ccggggaaac cctgacgccg gaggaaacgg
    30181 cctatttggg ggcgcttctc tcttatgtgc aggagtgccg ccccatgctc aatcagggcc
    30241 agtatcagtt gccggagccg ccgaaactgg ctgacttcga ccagagcttg caggactaca
    30301 aagcacaggt acaggccgag atcgcgcagg aggccgccgc agccggtatg accgtggagg
    30361 aatacgcggc ggctggctat gaagcacccc aacaggacac cttttccatc taccagcttc
    30421 gaggcgggga ggaaacccgc gactatcggt ttgagccata cgagcgttta caggccgcag
    30481 ggctggccgt ggacagtgcc aactatgacc ttgtttatac ggcaccgctg gataaaagca
    30541 cgtctttgga ggatatttac cgcacattca acatcgacca cccggcagat tttaggggac
    30601 acagcctttc cgtttccgac gtggtggtgc tgcaccagaa tggacaggac accgcccact
    30661 atgtagacag cttcggctat atggaagtgc cggagttttt gcgggaaaat cccctgcgta
    30721 ccgccgaact gtctacggag cagaacgaaa acatgattga cggcattatc aacaatacgc
    30781 cgcccgaacc gcagccggag gaacccgaac agctatcttt tgaggatgtg gcagttgctt
    30841 ccggccagac acaggaggaa cccccggagg ctgcgccccc gcaaacggct gtccgctatt
    30901 atcccatcaa tgagggagcc gcccgccgcg ccaaggaagc catgagcttt tctgactacc
    30961 ggcccggcag cgccacggcg gaataccggc actatgtcga tcaggccgtg gaaattgcgg
    31021 agcgccagaa aaagcgtgtg gagccagagt atcacgaaaa gatagacagc ctccttgaca
    31081 cctacgcccg caagctggcc gagaacatga ataagggcta tgagattacc gcccgcgtcc
    31141 cctctgtcct gattgccggg ccgtccaatt tcccggtacg ggcgaaagaa aagcagaacg
    31201 ccgccagtga ccgcaacatg gaggaatttc agtacattca gggcttgctt gacaaaatcc
    31261 gcagcaccgg catgggtggt atcagcgccg acgatccgca ggccgtttcc aagctggaaa
    31321 agaaactgga aaagctggaa gcgtcgcagg agttgatgaa ggccgtcaat gcctactacc
    31381 gcaagcatgg gacgctggac ggatgccccc atctgaccga aagaggcatt gaaaacctga
    31441 aagcggatat ggcgtcgggc tggcactatg agaaaaaacc gttccagtcc tggcagctct
    31501 ccaacaataa cgccgagata cgccggttaa agggccgcat tgaggaattg acccgccaaa
    31561 aggaggcggc ctatgtggga tgggagtttg acggcggcac tgtggagatc aaccgggtgg
    31621 ccaaccgcct gcaaatcttc tttgagggga agccggatgc tgctgtacgc gacgagctga
    31681 aaagcaacgg tttccgctgg tcgcccaagg ccggggcatg gcagcggcag ttaaacgaca
    31741 ccacaatccg cgtcgctgac cgtatcaagt gcatccagcc cctttccggc gaaaagccca
    31801 gcgccttaca gcttgcggcc cgcagggaga aagaaaagcc ctctatccgg gcgcagctca
    31861 acgcggccaa gaccgaacag gccaagcagc caccggcgcg ggaaaaatca aagggattgg
    31921 aggtagagta aatgcagaag tttgaaaatg tggacgttct ggccgcgctg gaacagatca
    31981 tgcggcagaa caccgcgttt tatcaaagtg actttggcat tgacaaagac attatcaggc
    32041 gggatgccgc cagcgatcag gccgcagaca agaccctgct ctggatgtcc cggccctccg
    32101 ggacgtactg tttccgggaa cgtgatgttt tcctgaaaga cacccgacag tacaacacat
    32161 ggaaatttta cggggagcag acccgtgaca agatactggc ctacgcggtg gagctgaccg
    32221 gcacacaggg cggcactatc cggggcaatc tctatgagct ggactatccg cagcatttcc
    32281 ggcacgtcat tgaaaccgcc cagccggtga gtgtgaaccg gctcttttat gagcatggca
    32341 cccgtgacat cccggagagc cagcgttttg acggaagccc tgaccgggta ttgggaaact
    32401 ttttgtacta tgaggcccag ccccatgacc cggctgtgct gcaggaggcg ctacggacgg
    32461 agcagcacag ccgggggcgg gccgtccccg gcgatttcaa agtccacgtc accgctttgc
    32521 atgacagccg gattgaaacc gaggcccggc gtattgtgga cagcctgaaa gatctgtccg
    32581 cgcctaacag ccccaataaa acgcatttca tggtggagat ctccccgcac tttgcggcga
    32641 ttgcttccac caaggataat gaccggctct ttgccatgct gccctataaa tccctgtgtt
    32701 ttaccgggat gaaaggcagg catggcgtgt atgccgtgat taaccgggac gagcgccggg
    32761 atgtgagtat tcgcaggccc cggccctcta tccgcaagca gctatccgac agcaaacagg
    32821 ccgccagccc aaaaaaggcg gcggccagaa ccaaaaagaa tgaattggag gtataaggaa
    32881 tgatgcactt taccgtggag gaagaaaacc tgatctgtat gtaccacaat gcagaccggc
    32941 gcagaacgat aaacaggctc accgccgtca tgccggttat ggatggggat atgcgggcgc
    33001 aggcccagca gaccctttcc aagctggaac gcatgacgga tgccgactat gacggccaga
    33061 agttccactt cacagacgaa taacgaacag cagacgccgg gcgcggcggc catgtggtcg
    33121 cttgcgtccg gcgtcttttc tttttgccta ttcctacgca tttagaaccc ggtattgtcg
    33181 gggataggat gaagtattcg cttgcccctg caaacatgcg ggagaagcct tgcagcgggc
    33241 ggttttcaat cccttaaatg cgtaggttgc agctataatt agacccctga aacagatata
    33301 gccgcgcagc gggtgtatct gtttcagggg tgtgcaggtg atagccgcaa agcggcgtaa
    33361 gagggtgcag ccctcttgcc gtctatctct gttccagcgg gaagctcggc aatgcttcca
    33421 gcagtttcag ggaaatgcgc tgccgtatat cttcgtcaat gactttcttc tttttgccgt
    33481 ttgcctgtct gatctccacc gtggaaagct cgttgatgta gtcagcataa tgggccaaca
    33541 ctttttccgt ggcccacctc tcaccggcaa cggcggcttt gatcgtttca tagtccagca
    33601 gcttttcttg tccgtccatc ctgatacccc cgtcagactg attttttcta tgataccata
    33661 ttcggaagca tggaaccaga gaaaacggcg cttttctgct ggtaaattct acgcatttag
    33721 aacagcactt cggcggggat aggataaaac acccacatac cccgctgacc atgccggaaa
    33781 agccttgaaa tataagggct tttacagctc taagtgcgta ggtttccagt atcttttccg
    33841 ctggcgctcg gcctctttgc ggcggcgcat ccgtttggag caggccgcgc agtatttggc
    33901 ccggttagac ttcggcgtga acgtggcccc gcactcacag cagcgtttca cttcgtcccg
    33961 gtctttggtg atctcggcat acagggccgc atccagcggg aggacagcgg ccttaaacca
    34021 gcggcacaga agcgaatagg aaatgctctg tacgcaaata cattcttcgc cgtcgtccag
    34081 caacaggcag ttgccgtcac aatagttgca gcactcatgc gtcagcttcc gggccttgcg
    34141 atattgaggg taattcattc gtggaatact catagctctt gcgtccattc ctttcccggc
    34201 gtcatacgca aaatgccgtc cacattctgc ttgatgatgc cgtattcctg catctgcttt
    34261 ttcgcttcgc cgtacttctc atacagccgt tccttttcct cggccaatcg cttgtactcg
    34321 gctttcaggg cgtacagatc ggggagcttt ttcacaccct gtccctttag ggcctttgcc
    34381 gcagcttcat acaaaatcaa cgtgctttca tgctcacgcc ggaattttgc cttatccgct
    34441 gcattgcggt attccatagc cacgggccag agctgcttgt aggtagaaat attctttatc
    34501 agcaccgcca tatccgcaag ccggtgttcc atagctttga gggtagcggc ggcctcgtcg
    34561 ctggcggcgg tgatctcggc cactttgctt tccaaatccg ggtaggtgtc tatcccatgt
    34621 tcagtcagga aattcaaagt ctttgcggcc tgcttcaaat tgtggagctt cgcccacctc
    34681 tcatagcctg ccgactgctg ggccttaatg ttgttttcca gatcaatccg caaagaaatc
    34741 cccttgcgct ctttgggcgc tctggtgata acgcggcctt tgatacgctc tttgatggct
    34801 tcctcggtgt aggcttcccc caatgtcttg cagcgggtaa agcgttcctg tccgggagcg
    34861 cggaaagaaa taaactttcc cggcttgatc tcatagccct gcgcctgcat gagccgcagg
    34921 aaatcatcaa aatcttttgc ctgcgggata gcggcgtcaa tggctgcttt gagcttcgct
    34981 ttccagctcg tccctttccg ctgggcgtcc cactcggcat agctcttgcc cttactctgg
    35041 ccgggcttca ccacggacaa tccgttttcc ttgcacagcc tgtcgctggt gcgccggata
    35101 taggcatagc tctgtttgtt ggaaatgtac ttgcgctgat ttaccatatc cacggcacag
    35161 aaaataatgt gattgtggac gtggccctta tcaatatggg tagtcagcac atagggatat
    35221 ttcccttgca gcacttcctc ggcaagctgc ttcccgatct catgggcctg ttccggcgtg
    35281 gcttcgccgg gggcaaagga ttgtatcagg tggtggccca gattattgcc cttttggtat
    35341 gcctgcgcca gcagcatttc aaattcaatg tccgctgttt cataagagca ggcaaaggag
    35401 gccaccagca gcttgccgtc agttttgtcc gggttctcga tgtagtcaag ggcttttttc
    35461 agcgttgact tgataggatg tatctttgta actgccatat ctgcgccagc gcccccttta
    35521 tatcctcgat gtcctgtgcg tacaccggcc ccataccgtt cagcctcttt gcaatctgat
    35581 tgacgttgac gccgatcttc tgtatctcgg cggtctgcgc ccggatgtcg ctggtgtcca
    35641 tgttgacgat atagccgtca atgagcatct tccgggcgta ggctgaaaag ttcttcgtat
    35701 gcagcagcgc cattttctgt tcaatcatag cccgttcctc cggcgtcaca gggacgcgca
    35761 gcacgatttt tcgctttctg tttgccatgc ttcaccgtcc tttccattca ggggtttggg
    35821 gatttcccca acaagcaaaa cggacgcggc ccggcaggga cggggcaggc cgtttttccg
    35881 ggagtggcta cactccctat gcttgctacg ttactgtttc tccactaaca agacgtttcc
    35941 aaagccccaa actacccgca aaatagccct ctggaaagaa aaaagtgcaa aaaaatagaa
    36001 gctacgctgt gcagctcccg ctaaaagtaa aacagaggat gccagttaca catcctccgt
    36061 ttcattcttc ttcggctttt caattagtat ggcgctgtca atcgcgccct cgatgatcgg
    36121 caggtattct tcggggcaga gtttcagctt gtggctgacc ctttgccgca gttcgctttc
    36181 ctcccgcatg atctccgggt taaagtagcg ttccacagga agcccgcagg ctttaataag
    36241 ctgtatgaca accggcagac tgggaatggc cccgtcgttt tcgatgttgg caagatagcg
    36301 ccagtcaata tttattttct ctgccattgc tttgcgcgaa acgcgctttg cttgccgggc
    36361 cgctttcaca tccgcgccga aagtttcaaa gccgggacaa tcttcaatat tcgccatatc
    36421 gcacctccca cttacattgt atatttcata ctctgcccgt ggaatgttgg tatatgcacc
    36481 tttcttaaag tttatatttc acattttgtt tcttttattc ttcgagcaaa agaattataa
    36541 tgtaccctgt ggaggtgtac tatggactac atgacgttga aagaggcagg cgagaaatgg
    36601 ggtgtgcccc cacgatggat aaactactat tgtgctgcgg gacgcatccc cggcgctgta
    36661 aagatggcga cgatctggct cattcccaaa actgcggaaa agccgattga caggcgtaca
    36721 aagaaaggaa aggcggttac tgaatagtat aatgaaaccg gcgcattttg gatatggttt
    36781 tgaagagaga gaaagcatgg tattttgtca cggaaaaact ataagatgat agatggcagg
    36841 ctgctccaga ccgataaaaa gtattctcat ctgaaacaga aagaaaagat ttacacatgg
    36901 atgtatgaag aagcgaaact ttatcatgac agaaatggaa aatgcccgaa acaaagacga
    36961 ggaagctgct gctgtatcag ccgtatatgg tagaattgaa aaggccggca tctggattct
    37021 ttatggtgaa gtctccagac attggataac tgctggtcta atgcgccttt gagtatgggg
    37081 tgattgattt taataccggg gaggtacggc tttttgagga aaatattctg ggatttacat
    37141 ttggcgatta ttacatttcc tgtaactgca aggacacgga tgatgaagat atgaggaata
    37201 tagacttgct gattttctac tgtcttgaac ggtattagga ctgaatctgc tgcgcggagt
    37261 gcggcaagag cgaccggagg gagtacacac aggccctgcc tgtgtgaaaa gccataccgc
    37321 aaggtgcatt cgcaaaaaag aggccgcgca ttttgctgag gcttggggtg tccccaacaa
    37381 gcaaaacgag aacgccgcag gcgggcaagt ggtggatcag aatatccgta aaggatattc
    37441 tgatccattg cttgccagta ttatcgggat gttttgacaa ccgctgtaat cgaaacatcc
    37501 caaacattaa gaaggtgtta attattatta tcttgattta attcgtttaa accaataata
    37561 tagatacata caccatgaaa agaagcgagt atcccaatga cggatgatta aaatccgaaa
    37621 agaaatcgct ttgaaactac agggaaacta cagactgtta tgttatcttc ttaaaatgga
    37681 gggattttta tgtcgatacg aattctactt gtcgaggatg atgatcatat ctgtaataca
    37741 gtaagggcgt ttctggctga ggcaggatat caggtggatg cctgcacaga tggaaatgag
    37801 gcatacacca agttttacga aaacacttat caactggtta ttcttgatat tatgctgccc
    37861 ggtatgaacg ggcatgaact tttgcgtgaa tttcgtgcga aaaatgatac tcccattctg
    37921 atgatgacag ccctgtcgga tgacgaaaac caaatccggg cgtttgatct agaggcagac
    37981 gactatgtaa caaagccatt caagatgcag attttactaa agcgggtgga agccctgctg
    38041 cggcgcagcg gtgcgctggc aaaggaaatc cgtgtcggca ggctgacact tctgccggag
    38101 gatttcacgg tactttgtga cggtacagag ctgcccctga cacgaaaaga atttgaaatc
    38161 cttttgctgc tggtgcagaa caaaggcaga accttaacgc atgaaatcat tttgtcacgt
    38221 atatggggat atgactttga gagtgatggc agcacagtcc acactcatat caaaaatttg
    38281 cgggccaagc tgccggaaaa tatcatcaaa accattcgcg gtgtaggata ccgattggag
    38341 gaatcataat aatggaaaga aaagggattt tcattaaggt tttttcctat acgatcattg
    38401 tcctgctact gcttgtcggt gtaacagcaa cactgtttgc acagcaattt gtgtcttatt
    38461 tcagagtgat ggaattacag caaacagtaa aatcctatca gccattggtg gaactgattc
    38521 agaatagcga taggcttgat attcaagagg tggcagggct gtttcactac aataaccaat
    38581 cctttgagtt ttatattgaa gataaagagg gaagcgtact ctatgccaca ccaaatgcca
    38641 atacatcaaa tagttttaga cccgactttc tttatgtggt acatagagat gataatattt
    38701 cgattgttgc tcaaagcaag gcaggtgtgg gattgcttta tcaagggctg acaattcggg
    38761 gaattgttat gattgcgata atggttgtat tcagcctttt atgcgcgtat atctttgcgc
    38821 ggcaaatgac aacgccgatc aaagccttag cggacagtgc gaataaaatg gcaaacctga
    38881 aagatgtacc gccgccgctg gagcgaaagg atgagcttgg cgcactggct catgatatgc
    38941 attccatgta tgtcaggctg aaagaaacca tcgcaaggct ggaggatgaa atcgcaaggg
    39001 aacatgagtt ggaggaaaca cagcgatatt tctttgcggc agcttctcat gagctaaaaa
    39061 cgcccatcgc ggctacaagc gttctgttgg agggaatgct tgaaaatatc ggtgactaca
    39121 aagatcattc taagtatctg cgcgaatgca tcaaaatgat ggataggcag ggcaaaatca
    39181 tttccgaaat actggagctt gtcagcctga atgatgggag aatcgtaccc atagctgaac
    39241 cgttggacat agggcgcacg gttgccgagt tgctgcccga ttttcaaacc ttggcagagg
    39301 caaacaacca gcggttcgtc acagatattc cagccgggca aattgtcctg tccgatccga
    39361 ggctgctcca aaaggcacta tccaatgtca tattgaatgc agttcagaac acgccgcagg
    39421 gaggcgaggt acggatatgg agtgagcctg gtgctgaaaa atgccgcctt tttgttttga
    39481 acatgggcgt tcacattgat gatactgcgc ttccaaggct gttcacccca ttctatcgca
    39541 ttgatcaggc gcgaagcaga aaaagtgggc gaaacggttt aggacttgcc atcgtacaaa
    39601 aaacgctgga tgccatgagc ctccagtatg cgctggaaaa cacctcggat ggcgttttgt
    39661 tctggctgga tttaccgctc acatcaacat tgtaaatatt taaaacttaa atgattttga
    39721 ccgacaggta taaccctgcc ggtctttttt tgctgctaca ggaaaactac agactggcta
    39781 cagggaaagt acagatgcgc ttgccataat aacaatcgta ccagccacaa atcgtagttt
    39841 tattgcaaag gaggcattcg atcaaatgga aaaaagcaac tatcattcca atgcggatca
    39901 tcacaaacgg catatgaaac aatccgtgga aaagcgggct tttctatgtg cgttcattat
    39961 ttcgttcaca gtctgcacgc tgtttttggg gcggagactg gcttccgtat tggaggcaac
    40021 acagataccg cccatccctg caactcatac aggcagcagc actgacgtag tggagaattt
    40081 ggaggaaaac gctcttgcca ccgccaaaga acagggagat gaacaggaat ggagcctgat
    40141 tttagtgaac aggcagaacc ccatccccgc acagtacgat gtggagcttg agcaactatc
    40201 aaatggtgag cggatagata ttcggatttc tccctatctt caagatttgt ttgatgccgc
    40261 aagaactgat ggagtttacc cgattgtcgc atccggatac cgaacaacag aaaaacagca
    40321 agaaattatg gatgaaaaaa ttgccgaata taaggcgaaa ggctacacct ctgcacaggc
    40381 taaagcggaa gcagaaactt gggtggccgt gccgggaacg agcgagcatc agcttggtct
    40441 tgctgtggat atcaatgcgg acggaattca ttcaacaggc aacgaggttt atagatggct
    40501 ggatgaaaac agctatcgtt ttggttttat tcgtcgctac ccaccagaca agacagagat
    40561 aaccggtgtg agcaacgagc cgtggcatta ccgatatgtt ggcatcgaag ctgccacaga
    40621 gatgtacaac caaggggttt gccttgagga atatttaaaa ccagaaaaat gagaaaagga
    40681 tgttatggac agaaaaagat tgacacagcg tttcccgttc ctgcttccaa tgagacgggc
    40741 gcagagaaaa atgtgctttt atgcgggaat gagatttgac ggctgtcgct atgcgcagac
    40801 gataggggaa aaatcgcttt cccatttgct ctttgaaacg gattgcgcat tatacaacca
    40861 caataccgga tttgacatga tataccagga aaacaaggtg ttcaacttaa agctggcagc
    40921 aaagacctta aacggcttat tgataagacc gggggaaacc ttttctttct ggtggctggt
    40981 acgccatgcg gacaaagata ccccctataa agacggcctt acggtgacca atggtaaact
    41041 caccaccatg tcgggcggcg gtatgtgcca gatgagcaat ttactatttt ggatgttcct
    41101 gcatacgcca ttgacaatta tccagcgcag aggtcacgaa gtaaaggagt ttccagagcc
    41161 aaacagtgac gagattaaag gggtggatgc aaccatttca gagggctgga ttgatttaaa
    41221 agtgcgaaac gataccgact gcacctacca aatatgagtg accttagatg atgagaaaat
    41281 catcggtcag gtgtccgccg acaaagagcc ccaagcatta tacaaaatta caaatggcag
    41341 tatccagtat gtccgtgaaa gtggcgggat ttatgaatat gcccaggtta aacggatgca
    41401 agttgcctta ggtaccgggg aaataataga ttgcaagctg ctttatacaa acaaatgcaa
    41461 aatctgttat cccctcccgg aaagtgtgga tatt
//
LOCUS       DACECY010000023        35684 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.024, whole genome shotgun sequence.
ACCESSION   DACECY010000023 DACECY010000000
VERSION     DACECY010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.024"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(12..2183)
                     /gene="copB"
                     /locus_tag="IWO38_001238"
     CDS             complement(12..2183)
                     /gene="copB"
                     /locus_tag="IWO38_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ0313337.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2203..4389)
                     /locus_tag="IWO38_001239"
     CDS             complement(2203..4389)
                     /locus_tag="IWO38_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ0313338.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="IWO38_001240"
     CDS             complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="IWO38_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ0313339.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4611..5051)
                     /locus_tag="IWO38_001241"
     CDS             complement(4611..5051)
                     /locus_tag="IWO38_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ0313340.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5125..5664)
                     /locus_tag="IWO38_001242"
     CDS             complement(5125..5664)
                     /locus_tag="IWO38_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAQ0313341.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(6279..7745)
                     /locus_tag="IWO38_001243"
     CDS             complement(6279..7745)
                     /locus_tag="IWO38_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0313342.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(8056..10137)
                     /gene="cadA"
                     /locus_tag="IWO38_001244"
     CDS             complement(8056..10137)
                     /gene="cadA"
                     /locus_tag="IWO38_001244"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ0313343.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(10661..11020)
                     /locus_tag="IWO38_001245"
     CDS             complement(10661..11020)
                     /locus_tag="IWO38_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ0313344.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(11050..11391)
                     /locus_tag="IWO38_001246"
     CDS             complement(11050..11391)
                     /locus_tag="IWO38_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313345.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            complement(11388..12062)
                     /locus_tag="IWO38_001247"
     CDS             complement(11388..12062)
                     /locus_tag="IWO38_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0313346.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(13115..14413)
                     /gene="asnS"
                     /locus_tag="IWO38_001248"
     CDS             complement(13115..14413)
                     /gene="asnS"
                     /locus_tag="IWO38_001248"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ0313347.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(14453..15643)
                     /locus_tag="IWO38_001249"
     CDS             complement(14453..15643)
                     /locus_tag="IWO38_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0313348.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIETIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(15664..16191)
                     /locus_tag="IWO38_001250"
     CDS             complement(15664..16191)
                     /locus_tag="IWO38_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0313349.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(16238..19027)
                     /locus_tag="IWO38_001251"
     CDS             complement(16238..19027)
                     /locus_tag="IWO38_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0313350.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(19174..19374)
                     /locus_tag="IWO38_001252"
     CDS             complement(19174..19374)
                     /locus_tag="IWO38_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0313351.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(19826..20146)
                     /locus_tag="IWO38_001253"
                     /pseudo
     CDS             complement(19826..20146)
                     /locus_tag="IWO38_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20649..21767)
                     /locus_tag="IWO38_001254"
     CDS             complement(20649..21767)
                     /locus_tag="IWO38_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313352.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYYEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAIFRY"
     gene            22237..23544
                     /locus_tag="IWO38_001255"
     CDS             22237..23544
                     /locus_tag="IWO38_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ0313353.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSESKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANMFVIGWRRVAEFFGNLF"
     gene            23663..24861
                     /locus_tag="IWO38_001256"
                     /pseudo
     CDS             23663..24861
                     /locus_tag="IWO38_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24888..26156)
                     /locus_tag="IWO38_001257"
     CDS             complement(24888..26156)
                     /locus_tag="IWO38_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ0313354.1"
                     /translation="MEKQLPSTVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKAEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(26319..26966)
                     /locus_tag="IWO38_001258"
     CDS             complement(26319..26966)
                     /locus_tag="IWO38_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAQ0313355.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(27374..27877)
                     /locus_tag="IWO38_001259"
     CDS             complement(27374..27877)
                     /locus_tag="IWO38_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0313356.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(28000..28764)
                     /locus_tag="IWO38_001260"
     CDS             complement(28000..28764)
                     /locus_tag="IWO38_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ0313357.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DVEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSK
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            28872..29147
                     /locus_tag="IWO38_001261"
     CDS             28872..29147
                     /locus_tag="IWO38_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ0313358.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            29743..30693
                     /gene="yidC"
                     /locus_tag="IWO38_001262"
     CDS             29743..30693
                     /gene="yidC"
                     /locus_tag="IWO38_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ0313359.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(30872..31846)
                     /gene="lacD"
                     /locus_tag="IWO38_001263"
     CDS             complement(30872..31846)
                     /gene="lacD"
                     /locus_tag="IWO38_001263"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ0313360.1"
                     /translation="MKKMSIEKQECLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDESVEINVQKQIVIERIDSECEAEGMPFF
                     LEILSYDATISDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVKGYG
                     QETCYSQAEASTYFYEQSKVTEQPFIFFSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATPVYVDR"
     gene            complement(31843..32301)
                     /locus_tag="IWO38_001264"
     CDS             complement(31843..32301)
                     /locus_tag="IWO38_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0313361.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIAHDEKEVYRK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKTEIATILTKEIGEDI"
     gene            complement(32298..33761)
                     /locus_tag="IWO38_001265"
     CDS             complement(32298..33761)
                     /locus_tag="IWO38_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0313362.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEGLEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTILADNEAVDTLESETISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMLKTVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     SGGTGFLGGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVVFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPINKAAYVTGTALLAQGN
                     TTFMAGVSVACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEEGRAGGLS"
     gene            complement(33743..34693)
                     /gene="pfkB"
                     /locus_tag="IWO38_001266"
     CDS             complement(33743..34693)
                     /gene="pfkB"
                     /locus_tag="IWO38_001266"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ0313363.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQKKQIQTEFIEVPGMTRINVFTQVNQTREE
                     YKLVNQGPEIPQAAVHNFLNQIRALQAEDYLCVSGSLPRGMSPKVLVEISRICQEKEI
                     HLIIDSSDQEVMGCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENV
                     LLSLGEKGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLVGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            34836..35585
                     /locus_tag="IWO38_001267"
     CDS             34836..35585
                     /locus_tag="IWO38_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0313364.1"
                     /translation="MDHKLSETEQYLWNFIEHHILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFTDRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGETKELVTAAKNCYDAEIGSILVTANHSSTLKDYCEINLVGFKSEGS
                     YFPDYEVRSRLPLQIIARILLDAYVLRMREN"
BASE COUNT        10201 a         7672 c         6229 g        11582 t
ORIGIN      
        1 ttttttcatt attatttgat tttcaatgtc aaagcattta gagctacaac taccgtactt
       61 aacgacatca agacagcacc aactgctgga cttaatatga taccaattgg cgcaagaatt
      121 ccggctgcta gcggaattgc aagaatattg taacctgccc cccaccagag gttctggatc
      181 attttctttc gcgtttgctt ggctagatca agaaaatgca aaatatcttt tggatcgctg
      241 tttgtcaata cgacatccgc agaatcgatt gcgatatctg tccctgcacc gattgccatc
      301 ccgattgaag cacgggctaa acttggcgca tcattgatcc catcacccac catgattact
      361 tttttccctt ggtcaaggta tttctggaca acagcttctt tatcatctgg taataaaccg
      421 ccataatatt cttcgatccc taaataatct gctactgctt gggcagcgtt tttattgtca
      481 ccagtaagca tgacaggagt gatttttctt tctttcaatg ttcggataaa ttcttttgct
      541 tctgttttta ctacgtcacc taaagcaacg attgcagcta gatgattttc taaaatcagg
      601 aagctgatgg tatttccttg ttcttggtaa ggtttcaata atgcttgttc tacgtttaat
      661 ccgagacgct caacttcttt ttcattaact attttcactt gttgtttttt gactgttgcc
      721 tctagcccga ctcctgaaag attttttaga ttttcagctt gataaggctt gatttcatgt
      781 tctttcaaat aattcataat gccaacagcc aatggatgat tggcattcgc ttccaaggct
      841 cctagatatt gaaggatttc atttttattg aacgcatcat caagtacttc gacacccgtt
      901 acagtaaatt ttccttctgt tagtgttcct gttttgtcca gcatgatcac atctagatca
      961 ttggcttgct ccagtgcatt gcggtttttc aaaagtaatc cgtttttagc tgctattgat
     1021 gtggagcgag caacaactaa tgggatagct aaacctagag catgaggaca ggcgatgata
     1081 aagactgtta ccatacgttc taatgcatta ggaagatctg tcaagaacaa ccaggcgatg
     1141 aatgccagga tacccacaat taatgcgaca taaaacagcc attttgccac tttgtcagat
     1201 aatgactcta gcttggattt ttctccttgt gcttttcgca ccatttccat aaccttcgct
     1261 agataaccat tttcaccagt tccagttact tcgatttcga tcgttccatc cccattgata
     1321 gaaccgccaa tcactttgtc attggcttgt tttttgattc ctcttgattc gccagtcaca
     1381 gctgattcgt caacaattgt ttcacctttc aagattacac catctgttgg cattttatca
     1441 ccagaccgga cgatcaaacg atcaccttcg tttacttctt tcaatgaaac tgtctcttct
     1501 tttccatctt tcgtcaagcg tttcactgat tctggcagta attctgctag tttttgtaaa
     1561 gcattgctgg cattcgacac agcattcatt tcaatccagt gacctagtag cataataacg
     1621 atcaaagtag ccaattccca gaagaaatcc atgacgtgtg tatgtggatt gagcagattt
     1681 gcgataaacg aatagacact ataaatatat gcaactgtaa tccccatcgc gatcagtgtc
     1741 atcatagcag gacttttttg tttcaattcc atcttagctc cacttaaaaa tggctgaccg
     1801 ccatagataa atagtatagt tgctaagatc aatacgagcc aatcagaacc aggaaacgtt
     1861 acttgaaatg gaaattccat tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg
     1921 ataagcgata gccaaaattt ttgtttgaaa ttccccatgt gcatggaatg atccatgccg
     1981 ccgtgttcgt gatccatgtg catcgaatgg tccatttgtt tgctgtttgc tatatctcca
     2041 tggttatgtc ccatgtgcat ggaatgatcc atctgactat ggtcatgttt catctccatc
     2101 tgtttttcat gagattctgt actttctttg tttactactt cttttttatg ctcatgtttt
     2161 ttctccatat cgttcccatt catttttact ccatcccttc tcttactttt tcatgtgtcg
     2221 atttaagctc agggagttca acaatacact gacggaacta aacgccatcg ctcctcctgc
     2281 aatgattgga ttcaaaaagc ctagtgcggc aaaaggaata ccaatcgtat tatagacaaa
     2341 agcccagaaa agattttgtt tgatttttct caatgtcaac ctagacaact cgattgtttg
     2401 tgcaatggat gccaaactgc tattcatcag cgtcacatcg gctgtttcca tcgcaatgtc
     2461 cgttccactt cccattgcca taccgacatc agctaatgct aaggccggtg catcattgat
     2521 tccatcgcct gccataccga cttttttgcc atctttctga agtttttcta cgtaggctgc
     2581 tttatcctct ggaagtactt cagcaaagat atgctttgga tcaataccaa cttgtttacc
     2641 aatcgtttcg gctgccagtt tgttgtctcc tgtcagcata aaaacatcta atcctttatt
     2701 ctggagctgc ttgattgctt gtttggcttc tagtttgatt tgatcagcca cggcaatcca
     2761 cccgatcact tgcttttcat cagctaaaaa catgactgtt tttccctgct gttctaattc
     2821 taacgcgtgt tcttggtatt catcaaatga taggttcaat tcaattaatc gtttacgtgt
     2881 acccgcaaaa tagcgtactc cattaatcgt accgctgatc ccagcacctg ggtgggctgt
     2941 aaaatcaatc atatcgtatg ttgctgcttc ttgcagtttt ccgtattcca cgattgcttt
     3001 acctagtgga tgttcagaag cttgttctaa agtataaaaa atagatagga cttcttttga
     3061 accgactaga tctgtaactt tgggctttcc ttctgtaatc gttccagttt tatccaaaac
     3121 aatgctgtct aagtgagcag ctgcttctaa tgcttctccg cctttaatta aaattccatt
     3181 tcgtgcacca acacccgttc ctaccataat agctgttggg gtcgctaaac ctaatgcaca
     3241 tggacacgca atgactaata cagagacact atggatcaat gcgagttgcc aatcaccagt
     3301 gatcaaacct gtagctatga gcgtgataaa cgcgatgact aatacgatcg gaacaaaaat
     3361 ccctgagatc cgatctgcga ttttttggat tggggctttg ctcccttgag catcttccac
     3421 catttgaatg atttgcgcta atactgtctg cttgccgact tgtgtgactt ccatgtgtag
     3481 cagaccattc gtattgattg tcccgccaaa aagcgtgtca tccggattct tttctacagg
     3541 aagattttca ccagtaagca tactttcatc tatcgcactg cttcctgaaa taattcgacc
     3601 atctgctgga atctgctcac caggacggat gactagttga tcaccaacga ctacttcttc
     3661 aatcgctaac gtttcttctt tcccgtttct tatcacttgg gcggtttttg tttgaagaga
     3721 catcaattgt ttgattgcat tacctgtttt cgtttttgct gtatgttcca aatatttgcc
     3781 gagcaaaatc agcgtgatga tcatactgct gctttcaaaa taaagatctt gggggtgtcc
     3841 acggaaaaat ccgttgtaaa tgctcaatgc aaaggcagcc gatgttccaa ttgctaccaa
     3901 tacatccata tttggtgctt tcgtcttgat tgcatggtac gcacctttat aaaaacgagc
     3961 accgacataa aattggacag gagcagacag cactaactgg acgattggaa aatgaaaaaa
     4021 gtgaacaatc gccgcatggc ttcctaacat catagcaatc attgacaaca tcaacggcaa
     4081 agtcaaaacc gtactcaata taagatcgaa tagcattttt ttcaaatacg cttgcttttc
     4141 ttctgcaatt ttttgtttgt gggcttcatc gtacaaaatt gcaccataac cgatgttttc
     4201 tacgctttgg attaattttt cagtggtcgt cccttcatat ttgacacttg ctttttcagt
     4261 ggccaagttg actgtggcat tgaccacacc aggttgtccg ttcagctctt tttcgatcct
     4321 agcagaacaa tttgcacaag tcatacctgt aatgacgaac gtatccattt tggtttctgc
     4381 tgttgccatc aaatcacctc tgctttataa cccaatccat tgattgcttg acaaatcttt
     4441 tcggattgaa cttttgtttc atcaaacttg accgtaccgt ttgctttttt caaatttact
     4501 ttcacttttt gcacgccgtc aagtgcgctt actgtttctt ctactcttgc tgcacaatga
     4561 ttgcagctca taccttcgat tgaaaattgt tgtttcattt tttcttcctc ctattgatgt
     4621 tgtttacatt cacattgacc tgggatacag ttacattcga tcgtatctac tggttgcttt
     4681 tgttccagtt gtttcaaaat ctcattgata tcttcttgag tcaaagtggc ttcttctact
     4741 agatccgcaa tcgtctctcc aatccttttt gcacagatat gggaaaaaag attttccgtc
     4801 gcactgcgaa ctgtttccat ctccgagaca gctggatgat aaatgaattt ttttccttct
     4861 tgttctgtcc agatcacttc tttttttacc aaacgtccca gcaaagtttt gatcgttgct
     4921 ggtttccaat ccatcgaatc agctaagatc tgcgtgattt cttgggctgt cgtctgtcca
     4981 agtgtccaaa taacacgcat gatctcccac tctgaatcac tgatctttac tggcgttttg
     5041 actttttcca tttatttcac acctttcgtt tacaaatgta atcattagct tatgaataca
     5101 gattacgctt gtaaacaaaa actgtcaatt atttttgctc tctattttca atttagttaa
     5161 aaattcttgt ttctctcgtc ttgttttaag gatgatcagt ttatttcttg cattagctac
     5221 ttggatcagc tgctgtattt tcggttcatt gttttcttca aaatgccaga taaaagataa
     5281 aaattctaag tattccctgt ttagtttctc tgaaaatgat tcaggcatat ctggccgaga
     5341 ttttttgttt atctttcttt ttagagaacg ccaaactact cggtagatgg cgagataacg
     5401 cggcacttta agccaaatga tcttatctgc ttctttaatc ctttcctcta atgtagaagt
     5461 ataatttcct tcaacaatcc atgacggatt agataccata aacgattgct gttcttgcaa
     5521 aaaccacttt tttgcctgaa tactgtagtc tgttgtatgc cataaaaggt ctagcggcag
     5581 tagcggaaag tcggtgattt ctttgatctt ccttgcgagg gtcgattttc ccgacccact
     5641 ttgtccaatc acgatgatct tcattcttct ccctcacttc tctttggaat ttggttcgat
     5701 cataccactt ataacgccgc aaaaaaacaa gggactgtga caaaagtcgt gtcaaagtcc
     5761 cttgtttcga ataaatggtg ttcaatcagc tgaccttcag cattaactca aaaaacggca
     5821 aaatatgagg agctattttt tcgtttgttc ctctaatact caggtcaaat cgctgatttt
     5881 acaacctctt ccttaaacgg tgttctcctg ccaacacctc ggtcacaagg cacaatagtc
     5941 aacaaacatg agaagttgtt ttttgaaatt gctccttgtg actcggagtt aaacggcagg
     6001 ctcactacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccagca
     6061 aaacgaaaaa tatgaggggc tatttttgtt tggctgttct tattacttga tgcaggacag
     6121 ctttttcaca acctctgatt cacggtgttc cagaagtagc ttctgcgaaa aacaagccga
     6181 aacttcacaa aaatttgaga agcaattttc gtaaattcct tcttatttct tgaagctgac
     6241 cacttctgtc acaacctctt gtaacaagta tttctatttt aaggctgaac cactgtttct
     6301 ggttcagtga tttctttgtt tttgtctcga cgagcaattg ctggcaagat catacctaat
     6361 aagatcaaga caattggtgt gatgatattc gtgaataatt ggaaccacca tgctttagga
     6421 tcagcagtat aatctacttt tggcaccatt cctaggatac atgcaaacgc agtgaaggca
     6481 aagcaccaag caccaaagat aaaaccgatt tttggatttt taacaaattt atattcagaa
     6541 ttgaatttct tataagcttt attcaacatc atatatgcca agaacaccca taggtaacgc
     6601 attggcatga caactgagtt caagttcgtc aaccatttta ctaattcatt catatctttg
     6661 ataccaaaga tcggcaacaa aataatgatt cctactagta tacctgtcag aagataacca
     6721 ttcaccaatg tacctttacg attacgttta cgtagccagt ttggtacaaa ttctggatcc
     6781 gcatctgcta acaagatttt tagtggtgca tcaattgaaa aggctaatgc ggagatttgt
     6841 ccaatcatgt ttgtcaatgc gtaaatgatc atcaatgagt taccgatacc atagtaatta
     6901 cctaaaattt gaaaagcact gtatgcccca tttgccatca agtctttagg aatattatcg
     6961 ctagcaaaca gcatacccat tgcgactgag cctaatacag cacaaatccc taccattgct
     7021 gctaagaaga tcatcccttt aggaaattcc tttgcaggat ttcttgtaga gttgacataa
     7081 ggggatattt tttccgctcc tccaactgca aatactagca tcgaaacggt tgtgaaataa
     7141 ctgaaatcaa atgttggaat atatgttttc acttgattca tatgtggtgt ggcaaattcc
     7201 atcgttggat tgatgaacgg tgccgttaca gctaaaagaa taaataagat cgacatgacg
     7261 aacatcgctg ttccggccag tccgccaatc actttcaaag tagaaagtcc ttttgttgat
     7321 aaatataaaa aggcaagaaa tataataagt gaaataactg ctaccatttg tactgacatc
     7381 gtatttacga tatttccatt tccttggact gcccaaccgc cagcaatcag gattgcttga
     7441 ggcttttgtg ccaaataagg aatatggacg acccagtaag tccaagcggc atagtatgcc
     7501 agtcgtttat tagatgtcgt attttcgacc catgaactga cgcctccttt actatccttg
     7561 aaggctgacc ctaattgtcc aacgatcaat gcgtatggaa taaaataaat ggccaaaatc
     7621 aatagccaag aggtgacaac tgatactcct tgctgagcat agttattaac tacattcccc
     7681 atgccccaaa ccatgttaaa tgcaattaac gcaacagtaa accagcgtaa ttgtttgttg
     7741 ttcattcgtt ctctccttta tgataaaaat ttgttatcca gatgtaatca tattcaattt
     7801 ttaacaaact tacaagacag tgttatttta tgtgtgtatt cttttattta atacacatcg
     7861 ttttttcata ttttttccat caaaaaaata agttattttc ttttatttca tttaccttat
     7921 tgaatttcaa gctattttga attttctgca aaattaaaaa cattagttat tttataatcc
     7981 tatctatttt ctttacattt ttcattaaga aaaggatata aaaaaaagag gacgttcatt
     8041 cgtcctcttc attgatcaac gtttattttt tacatgcaag cttcgcatgg cattcaaaac
     8101 agctaatact gtcaccccaa catctgcaaa tactgcatct cccattgatg caaaaccaaa
     8161 agcacctaag gcaagtacca aaatttttat cccaatagca aagacaatgt tctgtttgac
     8221 gattttcaat gttttctttg ctaaatggat tgcttctgca atcttcgatg gttgatcatt
     8281 catgatgact acatctgctg cttcaattgc agcatcgctt cccaatccac ccattgcgat
     8341 tcctacatct gctcttgcta gcaccggtgc atcgttgatt ccatctccga caaatgcggt
     8401 ctttttattg tttctttgta agatttcctc taaccgctgc actttatctt ctggaagcaa
     8461 ttcgctatat atcttgtcca cgcctacttg cttcccgata tgatcagcaa tttttttcga
     8521 atcgcccgtc aacatgactg tgttcttcac accagccttt tttaattgaa ccaatgcgtc
     8581 tacggcatct tctttcaagg tatctgcaat aacaagataa ccactgtaac tttgatcgat
     8641 cgctagataa agcaatgtac cgatttcttg acttgaagga aaagagattt caaatctttc
     8701 catcaatttg tgattacctg ctaatacttg tttgccatca acttcagcta agatcccttc
     8761 acctgcaaat tcttggatat tagctgttgg taaaagcgga cctgcgtagc cttctaaaac
     8821 agattgagca atcggatgtg tagaaaactg ttctgcactc gcaacatatt gcatgaatat
     8881 ctttggatcg atttttgtat cgatcgtctg gatcgaaaag tcacctttcg ttaatgtgcc
     8941 ggttttatcg aataccactg tctcagtttg tgcaagcaac tccaagtaat ttgaaccttt
     9001 tactaataca ccgattttac ttgctcctcc gataccgcca aagaaactaa ggggtacgga
     9061 gatcactaat gcacatggac atgagatcac taaaaatgtc aatgctcgat agatccattc
     9121 agaaaacgcc gctccagaaa caacaagtgg aggtaaaaca gctaataaaa cggccaatcc
     9181 tacaacgact ggtgtgtaat agcgcgcgaa tttagtaatg aaattttcag ctggtgcttt
     9241 tttactgctg gcattttcta ctaattctaa taatttgctg atagtagaat tttcataaga
     9301 tttttctact tgaactaaaa atggtttatt tttattgata aatccactta atactgtttc
     9361 accaacatat acagatttag ggactgattc tcctgtaagt gctgacgtat cgactaatga
     9421 ttctccttcg atgatcgttc catctaacgg tacacgttca ccaggcttca caagtacaaa
     9481 ctgaccgatc tgcacgtctt cagggttcac ggtcatcaat ccttccgtcg tctgcacgtg
     9541 agctgtttct ggacggatcg ccatcaattc gcgaatcgat tttcttgaat gactgactgc
     9601 caatccttgg aaatactcgc cgatttgata aaatagcatc actgctacgg cttctggata
     9661 ttcaccgatc aacatagcgc caatcgtagc aacagacata agaaaattct catcaaagat
     9721 ttttcctttg acgatattca tcactgcctt cttcactaca tcatatccta tcagcagata
     9781 aatcaataaa taccctatca agcgaattgg ttcattaaaa tcaaataata gcaataaagc
     9841 gatacctgca agggaaaata aaatccggaa cagcgtattt ctctcttttt catttccatg
     9901 ttcagatact ttttccttat ctatttctgt tactttgaca tctggttcta attccttcac
     9961 tacttttttt atttcttgtt caatcgtagc caaatgatct tcctctgcgt caatcgttag
    10021 ttttccagaa gtgaagttta ccgttgcttc cttgactcca ttgatcttct gaactccttt
    10081 ttcgattttc attgcacagt tcgcacaatc taaaccttct aatctgtaac ttttcatttt
    10141 aatgacctcc tcgcctcaac atatgaatat ctcttcataa ctaattataa atgaacggtt
    10201 attcatatgt caaggtatcc gaaagaaaaa atgaaaaaca tctgcatcgg aaaatttctt
    10261 ccatacagat gttttggctt attctttctt tttcttttcg atcattgcca ctgctcttat
    10321 ccaacccgca cttgccttct tgatcttgac aggatagcaa ctaagtttga agcctgttgc
    10381 tggcaatttg tctaaattca ttaatttttc gataggacaa tatctttttt caatccctgc
    10441 aaaatgtcct tcccagatat tcgaagctcc actacgcttt caaaaatata gaaagagttc
    10501 tttaatgaaa attatttcaa ctttccataa acagtaaccc catataaata aaagacgtaa
    10561 aagtcacatg gaagaactta tacaggagaa cgcatagagt caggtgaaat acatgtatcc
    10621 cacctgactc tatgctttat ctaaccttta ttattctctt ttatacaatt tctgcaccga
    10681 atggcatcaa cgccaatcga gcaagtttga aagattgctt ggcaaaaggt atccctatga
    10741 tcgtgataca tagaaggatg ccgctgacga catgtgcgag agcaattggt aggcctgaaa
    10801 cgatcagcca gatgatatta acaatcaatg acatgccaga ctggtgataa attacttctt
    10861 tgccaaaagg cgcaaaagaa agggaggcga tcttaaaaca ttgcataccg attgggatac
    10921 ctatgatcgt gatacaccaa agtatccccg ctgctaacca gctaagacca ccgattagtc
    10981 cgccgaatat aaaccagatg acatttccta aacaactcat tgtatttctc cttctattac
    11041 tgtttttgat taaaactggg caaaaaatga ttttattctt ctttttttag cttccagtat
    11101 caaatattct gcaaattttt tttggatttc ttccgtattt tcttttgtta ctaatgtgtg
    11161 ttggattagt tttcctgatg gtcgctgatc caatcgttct tctgactgtt gcacgtccgc
    11221 ttgcgcaaat ccgataatcc gtttttcttt tgttacagca acgagcaata gtttattctt
    11281 atcatttagg tcagctgccg tttttgcttt tacgaatgat tctggatgat cagtaatcag
    11341 ctgattgatt tgaggtagat cttcttttac tgcccaccgt atttccatca tttctcactt
    11401 ttctttcgct catttttatc tccactaaaa aaatcgactg cggcaattgc atctatccag
    11461 ctttgtgcaa tgatctcatg tccgccaatc gttgggtgga ccccatcttc tcttgtatat
    11521 ttcgaaaaac cataaacaat ccctaaagca ttgaatatac catctaaagg aaccagcaat
    11581 gcgccatatt ccttagcaag tttacgaaca atttggattt tagggtctaa gtctttacgc
    11641 cagccatgac ggtcgtcggt tataggaagt acaaacggct ccatcaagat gatgttgcag
    11701 attcccactg tttgaagttc atttagtaat tttcgataaa cggattcaaa ttgctgagca
    11761 gatttctcag ttccgaatat agcctgatcg ctagtattat gccaggtatc attgattccg
    11821 atcaaaatcg atacatagtc aggttgtaaa gctaaacaat cttcttgcca gcgtacttgc
    11881 agatctgcaa ttttgtctcc tcctatccca cggttcatgc attcaaattc catcgttgga
    11941 tattgggaga ttagttttcc ggcaatcaaa agtggataac ctttacctag atcagttgct
    12001 tctgctcggt ctcttcctgc gtctgtaatg ctatcaccga taaataatat acgttttttc
    12061 atttgtcaaa aactcctttt tgaataaaac gatgattttc ttccttatat tatcccataa
    12121 gccctatcaa gacgaaagag aaaatatatg tataaaaaat gacaccagcc tagtcccttc
    12181 aaacaattga atgaacgctc atcaagtgtc tagtttttta tgctattttt ttcttaacgt
    12241 gccggattcc ccttgattcc ttcgttgttt ggtccagaga gatcgatcac acctaagctt
    12301 ccgtcaatca gaccactgat gacgccatca gaacatatat ttataggctc atctaagact
    12361 tctagtaaat gtccctttac taatatctct tctagattgc ttgggataga ttggtcataa
    12421 ctttatctgg tccgatgatt tcaagggtga attgaactta catatggatt attgcatgct
    12481 gaaaatacgg acctactcca tcttcgaatg ttactattag tttgttttca ggtgctagat
    12541 gctgctttat ttcaacagca gaagcatcat caaatcagat ttccatttga attaccttct
    12601 ttgtaaatgt ttgattgatt gcgatttcgt tatagaacct tgtgaatcaa atagcaattt
    12661 cttttgcttt cttctcttgt ctgctggaaa cttcctttat cccgtatttt aaaaaaagaa
    12721 ctgcgagagt tcgcagttct tttttagaat aaacggtgct ctccggccaa ctcctcggtc
    12781 acaagtcaca atagtcatca aacatgaaaa actgtttttt gaaattactc cttgtgactc
    12841 ggagctgaac ggcaggcgca caacctcttc cttaaacggt gttcaaaagc cagtttctcg
    12901 gtattaagcc aaatttatgc aaaaatgtgg aaagccattt ccacaaaatt tttcttaata
    12961 ctcgaaacta aacagctttt tcacaacctc tccactaaac ggtattcaaa agcttacatc
    13021 tgcggtaata agtcccgcca attcaaaaac atgaacagct gtttttgctt ggctgttcta
    13081 attacttgat gcagaacagc tttttcatca cctcttatgg ataaattcgg ttcaacaaac
    13141 gtgggaatgg aatagcttca cgtacatggt cgataccagc aatccaagtg actgttcgtt
    13201 caagacctag accaaaacca gagtgcggaa cagaaccgta tttacgtaga tcaagatacc
    13261 atgaatattc tttttcatcc aaaccgtgat tacggatttg ttctagcaag aaatcatagt
    13321 ctgttgcccg ttcgcttccg ccaataattt caccgtaacc ttctggtgcg atcatatctg
    13381 cacagatcac cacgtcttct cttgttggat gtggtttcat gtagaatggc ttgatggctt
    13441 ttggatagtt taggataaag acaggctgtt cgaatgaatt cgcaatgaac gtttcatgag
    13501 gtgaaccaaa gtcttcgccc cattcgatgt catcaaatcc atttttcttc agtaattcga
    13561 tcgcatcatc ataagtaata cgagggaatg gcaattttgt ataagttttt aagacttctt
    13621 tgtctcgtcc tagtacatct aatgcatatt cgcaatgatc taaaacattt tgtactaaga
    13681 atgctacata ttgttcctgt acttctaaac tttcttcttg atgagtgaaa gccatttctg
    13741 gttcgatcat ccagaattcg atcaaatgac gtcgtgtttt tgatttttca gcacggaaag
    13801 tcggaccgaa tgaaaagact tttccgaaag ccatagcagc agcttccatg tataattggc
    13861 cactttgaga taaataagct ttttgatcaa agtaatttgt ttcgaataga tccgtagtgc
    13921 cttctggtgc agaaccagtc aagatcggcg gatcaatttt gacaaaatga tgttgattaa
    13981 agaattcata cgttgcacga atgatttcat ttctgatctg cataatcgca tgttgtcttg
    14041 aagaacgcag ccataaatga cgatgatcca tcaagaagtc tgttccgtgt tcttttggtg
    14101 tgatcggata atcgtggctc tcgcttacca cttccacatt tgtaattccg atttcataac
    14161 caaattttga acgactgtct tcacggattt ctccagttac taacacggat gtttcttggt
    14221 gtagattttt agctgtttgg aaaacttctt ccgacacttc gctttttaca actacacctt
    14281 ggaaaaaggc tgttccgtct cttaattgta aaaatgcgat ttttccgctt gatcgcttat
    14341 ttgcgaccca agcgccgatt gttactgttt ctcccacatg gtttttcgaa tcgatgattt
    14401 gaatttgttc cacgtatgac tctcctttta tattccttga atttctttag gattaatttt
    14461 gacttttact ctcaataaat tgcttgattc tgcgtaccgc ttcttctaac gtagcgcgat
    14521 cagtagcata actcatgcgg acattttctg gtgccccaaa gccttcgcca gtcacaagcg
    14581 ctacccctgt ttcttctaaa agatcttcta cccattgtgt gacattttca tatttacaca
    14641 tgcgtagcgt ttcttttaca ttaggaaaaa gataaaaagc tccttgtggc ttttcaagag
    14701 aaacacccgg cagttctgcc acaagcgaat ataacgtatt caggcgttct tcaaaggcgt
    14761 tccgcatttc ttccactgta tcttgctcgc ctttcaatgc ttcaacagct gcatattgac
    14821 tgactgctgt cggattactg gttgactggg aagcaacagc gatcattcca ttgatgatca
    14881 caggatttcc caccgcataa ccaatgcgcc agcctgtcat cgcatatgtt ttagacacgc
    14941 cgttgatgat gatagtctga tttttgattg cttctgaaat cgttgcaatt ggagtaaact
    15001 cattgccatt ataaactaaa cgaccataaa tatcatcagc aactatcaaa atatcatttt
    15061 ttactgccca ttcaccgatt gcctgcagtt cattcttgct ataaatcatt ccagtcggat
    15121 tcgatggaga attgatgatt acggcttttg ttttttctga acgtgcttgt tctagctgtt
    15181 caacagtaac tttccagcta ttggattctt cccctttcac aaagacagga agtccttctg
    15241 ccagttttac ttgctcaccg taactgaccc agtaaggcac aggaatgatg acttcatctt
    15301 gcgggtcaag gatcgtttgg aaaagggtat acaaagcaaa tttcgctccg tctgttacga
    15361 ctgtttgtga aggttcatag acaagtccat agtttttctt cagataatca acgattgcct
    15421 gtcgaagctc tgggattcct gctgttggtg tataatagct agctttccca ttgcggattg
    15481 tttcgattgc tgcttcttgg atgttttctg gtgtcgcaaa atccggttct cccacagtca
    15541 aactcagcac atctctgcct tttgctttta atgccttggc ctttgccgca gctgccaaag
    15601 tgacagacgg ttctaatcgt tttgttcttt ttgataactc catcccattc attcctttac
    15661 cttttaaatt cccttgattg tcttgacttc atctccagat tcaaagccaa tcaggtaata
    15721 attcagtttc ccatttgttt cttttcctac tacttcccac acaggctgat catcatacat
    15781 ccctaaagca gctttttcga tttggatttc aggatgggcc gcagcaattg tctgttttgc
    15841 ttcgttttca gtcagtccct ttgtttgatc caatactttt actttttcac cagatttagg
    15901 aatgataacg gcgatttctt ttcctttatc atttgtacct gtcagtgaaa agtatgtctt
    15961 ctttcgagta aaccaataaa aatgatcggt ttcctcaaga tttgcatatt tctttgcgat
    16021 ttctactgct tctttcttcg cctgcaccgc cggacgactg gaacgcaagt aaaaaatcga
    16081 gctgatcacg atgaccgcta ataaaaatac gatcaagcca agtaacacgc gattaatcgt
    16141 tttttccttt gtctgttttt tcttcaaggg tcgtcgctcc tttatcttca ttccttttca
    16201 ttatagcaga ttttaattat gaaaaaagac agctcctcta acttccttct tcatttttct
    16261 ttaagaattc atggatatcc tgatagattt cctccatagc tagctctttg atcggtactt
    16321 ttttcggtaa tgcatggatg atccgtgcgc catattttgc agtgactagt cttcgatcca
    16381 aaataagcaa tacgccacga tctgtctccg tccttatcaa gcgacctaat gcttggcgta
    16441 acttcaaagc tgctttaggt acggattcct ggtaaaaagg attgatcccc tctgattcta
    16501 aataagcgtt cctagctttg accatcgggc gaagcggatt atcgaagggt aaacgtgtga
    16561 ctacaacgat ctgcaaagca tctccaggca aatctacacc ttcccaaaag ctgtcagcgc
    16621 caaacaatac gcttttttct gacaaaatga agcgtttcaa aagcttctcc cggcttccac
    16681 caaacccttg agctagtatt tctcgtcctt tttctagcat agacagatgc attttctgat
    16741 agaccttctg caaaacatca tgagaagtga ataacacaag tattggccga tcttcttgtt
    16801 cgatcagacc tgtaagtgct gtctctaaat aatcgatata tgcttgattg ctgacttgat
    16861 ggacggcagg tgcctctttt ggcacataga tccttgcctg tttctcataa tcataaggag
    16921 aggcaatcac tttaagaggt gctgcttcaa tgccaagcct cttagagaga tagtgtcgat
    16981 tagggccgat cttcagcgtt cctccaatat aaagaatacg atcatatcga ggataccaaa
    17041 cagtcgacgg caacatagcc gcttgaaaat catggagatg aaaaatcatc tgctgcgctg
    17101 tgttcccagg aacgaaccaa tggatcaaat gatctcgcca gtcttccagc cattgatcca
    17161 tcagcaagta ttgcttgtct aattgatcaa ataggctata taaacttgct aaatcgatcc
    17221 gcatctggtt tgtccagctt tcttggataa tctcaaaagt cagacgcatt tgtgactgta
    17281 gctgctggat ctcctgataa aaaagctgca ggtgatcaat ggctgtccca gattccagtg
    17341 gcagctgatc caacatctct tttgtcatca tgacttcttc tggatactgt ccgttaggtt
    17401 gcttgccgct cactaaactc attaatcctt ctgccataca aatctgtgct tcgcatagcg
    17461 cagaaagttc ttctcgataa atcgataacc agcgtaaggc ttcatgttct ttcggtaaat
    17521 agctttgtat tcggtcaaac agctgatttg gttcttctaa ttgctggatt tgtttatgga
    17581 aatagttaat acttaatcgt ttgtcattta ctttttcagc gatttccggc aaatgatgta
    17641 cttcatcgat caacaaaaat ggactagctg gaagcaatgg ttcttttcgc tgtgtttctt
    17701 gtactaaaaa cgcatgattg acaatcaaaa aattactttg cctgattttc ttttgcaaga
    17761 aacgaacaaa gtcttgttcg taaaatgact gatttcttgc taaaaatcca atacctctgt
    17821 gcgcgatatc tgcaaagaat gaatggttca aatttgttaa atgtaattca tccaaatcac
    17881 cagtcttcgt ttgcgtcagc caaactaaga ccgccatttg atacaaagca tattgttttt
    17941 gttctggcgg ttccacaagt gtgcctttaa aacgctgcaa atcaatatag tggcgatagc
    18001 ttttgataat tacaggttgt atgggctgat ctagcaattg attcagcaat ggaatgtctt
    18061 tttctatgat ctgctgctgc aaaactagcg aaacggtact gatgacagcg ggtttttcag
    18121 gtgtagccaa ataactcaat ggcagcagat aaccgattgt tttacccatt cctgtagctg
    18181 cttcgatcat caaatctttt gattcatctt ttgtgaaatg gtcgtagacc gcattcatca
    18241 gtctgttctg ttcttttcga tacatcagtg ttttaccaaa cattttttct tttgcttgtt
    18301 tttttcttgg atatgtccgc tcgccataat ggacggatgt gaatagttcc acttcttttt
    18361 tctgcaaagc tagcccatcg acgatttcta atgagggatc aagtggctct ggtttttctt
    18421 tcatttcttc aatcacatga taaatgaaac gactcgtatc catacctgtg tgtctactta
    18481 atcgcgcaat ctgctctaac gtgatgattg gcaattttct catccgttct tcgatcagca
    18541 aaagaagttg acctgtcact tcggcatcac tatctgcctg atgaggattc tcatgaataa
    18601 atccaaggct ttctgacaaa tcagctaagc gaaagcttgg ttccgtcggc aagaaaattt
    18661 gtgcaagttc tactgtatcg atgcctggta tcttcaaact aggaagtccg catcgcatga
    18721 gttcatggtt caaaaaatta taatcaaaat aaatattatg cgcgacaaat atggtgtctg
    18781 ccaaaagatt gtaaatcgtt tgggcgacat cttcgaaata aggcgctttt tggacgcgtt
    18841 gattcgtgat ccctgttaaa tgctggattt gttttgaaat cttccgccct ggatgaatat
    18901 cgatcgaaaa acgtgaagtg atccgacctg attcaatcat gacgcagcca aattggatga
    18961 tccgatcttc ttttggattc gtcccagtcg tttcgatatc aacgactgca taagttcttg
    19021 gtttcaaaat atcttcctct tctcctgcct ctaatgttct tttgaaatta tactacaaag
    19081 tgcaaaaaaa tactctcatc gctttttaaa gaaaatagac aaaaacgccc agacacgcaa
    19141 atgtctgggc gtttattttt tagtataggt taattaagct ttagtaacgt ttgttgcttg
    19201 aggtccgcgt tggccttctt caacatcgaa agttactgct tggccttctt ctaaagtttt
    19261 gaatccgtcg ccttggatag ctgagaaatg aacgaataca tcgtttccgt tttctgcagt
    19321 gatgaatcca aatccttttt ctgagttaaa ccattttact gtacctgttt ccataataaa
    19381 aatcctcctt gtgtatcaac acatattttg caattacttg aatggtgtat atctggaaga
    19441 tgtttatttg aaacaagcta ccacagatta ccgaacaaaa actactccta tagtatatca
    19501 taccgtttac aaaaaagcta gagtttttcg acttttttta ttaaaaaaag ttttctcagc
    19561 gattacataa agtgaagaac cgaacttttt ttaaaataaa cgacatattg ttcagttggt
    19621 gtttttttgc tctgatgttc ttctttaaac taaaaaaatt attctttctg actacttatc
    19681 ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat gttctcatgg
    19741 tatttctgat agcctatgac agtgaccaga agaacaaggc aaaccaatac gatcaacgag
    19801 attagttttt cttttttgct catatctata cctcataaat aacaggatga tgtccatcat
    19861 atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa tgccagcaac
    19921 tttcttaatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc aaagaccgtt
    19981 cttctgtttc aggaatcgca gaatcaagat ttggtgtctg tacataaaca tttttgtaca
    20041 gacgtgtgcc tgtaatagga gaccgctgtg tttcttcagc aaaaactggc tgacttgtta
    20101 aacttaggga tagaaacagc aaacaccata acagtctttt tttcataaag acttccttat
    20161 cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca cattttgttc
    20221 ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg tcacaacctc
    20281 ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt tcacaaaaat
    20341 ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact tctgtcacaa
    20401 cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca ccaaacaaaa
    20461 aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca gctttttcac
    20521 aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa aaacggcaaa
    20581 atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca ctgatttcac
    20641 aacctctttt aataacgaaa aatcgctgtc agactctttt catctggcgc atctttttga
    20701 tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc attcaacact
    20761 tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa gtttgacgta
    20821 gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc aactaactga
    20881 tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc tgcgagttca
    20941 cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc aggagaagca
    21001 gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa catcgtctga
    21061 ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa agaatccttc
    21121 aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc ttcatctttt
    21181 ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt agaagagaag
    21241 ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac cggtgcccct
    21301 tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt catggagtcg
    21361 cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat aatagccagt
    21421 aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa acgatggata
    21481 taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg ccaaaacgaa
    21541 ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg ttcgtaatat
    21601 tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat ctgatctttt
    21661 tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg tcctttagcg
    21721 ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa cgtacccctc
    21781 ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt tttccataca
    21841 ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc tattttttta
    21901 agttatcttc ataatttcct caattattta gccccagtct tcaactattc ttcatgaaat
    21961 gctgataaac taagaaaaga ataaaagagg tggatcttat gcatttgatt atgattttca
    22021 gtttattaag tattatcagt ttatttgctt tcagcgcttc ccgcttatta gatgcggaaa
    22081 cacagcctat tcctgtaaaa gtacgtaaaa acgaccgcta accttccata acgtgcgttc
    22141 ttatcctaaa gttttttcag aaacattgaa actctctccc ctctctgata aaattttaat
    22201 ttgtagcgaa acaaattaat tacaaggaga tcggatatgt ccataaaaaa aacacgtcta
    22261 gtcctattat tggctagtct cattctttgc cttgcttctt ttctaccagc tgttgctgtg
    22321 caagctgaag atacgttcaa agtaaatgca aaagcagctt ttgctgtaga tgcgcaaagc
    22381 ggtaagattc tttatgatca agatggagaa aaaaccatgg ggatcgcttc aatcacaaaa
    22441 atcatcggtc tttacatcgt tcttgatcaa gtaaaagaag gaaaactatc atgggatgat
    22501 aaagtttcga tttcagatta tgcagaaaat ttgagtatca ccccagattt atcgaatgtt
    22561 cctttgcata aagaaaacac ctacactgtc aaagaattat tcgactctgc aatcatccag
    22621 tcagccaatg cttcaatggt ggccttggca gaaaaaatct ctggcagcga atctaaattc
    22681 actgaacgaa tgaaagaaca attgaaagat tggggaatca aagatgcaac gatcgttaat
    22741 gcatcaggac tgaataactc ttatcttggc gaaaaccgac cagaaggtac tggagaaaac
    22801 gacgaaaatc aaatgtctgc tcaagatgtg gccatcgttg cccgccattt gatcttggat
    22861 tttccagaaa tactggacgt atctagcact acgacacaaa tgtttggcga aaacacgcaa
    22921 tcaccagtgg aaatggtcaa ctggaactgg atgctcccag gattcgttaa ttacaaggaa
    22981 ggcgtggacg gattaaaaac tggtacgact gagcttgcag gtgcttgttt cgttggtacg
    23041 atcactaaaa acgatcaacg gatcatcact gtcgtattga atgcggacgg ccatgcggaa
    23101 aacccgagtg cccgcttcaa tgaaacagac cgtttgatgg actactgcta tgacaattgg
    23161 tcagaaaaag agctcggaaa agctaacgca agtattccta gcctaaaaac cattgatgta
    23221 aaagacggaa aagaatcaac agttcctgtt gtgcttaaaa gccctgtaaa agtatgggta
    23281 cgaaacgata tggatactgg caaattaacg atcacgccaa cacttgacaa gaagcaagtc
    23341 actgacaacg agatccaagc acctatggat aaaggtacaa aaatcggtac cgctacgatc
    23401 accttggcag acgataaatt aggctattta gaagacaaca atgcgccatc aacagagatc
    23461 attacggata aaacagtgga aaaagcgaat atgttcgtga ttggctggcg acgcgtcgca
    23521 gaatttttcg gcaacttgtt ttaattgaac aataagaaaa gcatctttcc atcggaaaga
    23581 tgtttttttg cttttactag tttaaataac agcttttttc atctattttc gttatactgt
    23641 ataagaaaaa ggagttgaat ttatgaatct aaccagagat ctgatctttt tagttctcta
    23701 tcttattggg gtattctcta tcgtttattg gcgaaaacag aagaaggatc cttccttgtt
    23761 cttttacggc agtacgtgga catttacggt aatcttaact tattttgtcg tgttggaaat
    23821 tttttattcg accttttatt ttttgattcc attgttgttc tttagtatct ttgctttttc
    23881 ttatttcaca gaaaaacgaa ggctgctgaa tggtctttta ttcaatgttt tcctcatcag
    23941 tttcggcatc tacttgtttg tcttgctgta tgaaacgcaa aatattttcc ttggcggact
    24001 gatcgccttg atcacgattc cattgctact tgtcctttta tttggtattt atggattgat
    24061 tgtttttctt ttttggaacg gggtcacagt tttacgtaga gaatcgcatt cattagctaa
    24121 tcttttaacg ttgatactag ccatttttct tacactgttt cttgtttttg actttttctt
    24181 gttaaaatat ttgccgcaat ggataaatgc tttgtttttc tgcgttcccc tgatcttgat
    24241 ctatctattt atcgtattct acaacttttt gactgtctca ttcttgtatc agttcaaccg
    24301 gcctcgatac aatcaagact tcatcgttgt gttaggcgct gggttgatca atggagaaac
    24361 agtttctcct cttctagcca agcgaatcaa taaggcaatt gctttttatc gggctcaaag
    24421 ccgtgctaca ttaaatccgc ccatcttatt gatgtctggc gggcaaggag cagacgaaaa
    24481 agtaccagag gccattgcga tgaaacaata tgccatggaa caaggaattc ccgaaagaga
    24541 cattcttgtc gaaaccaatt cgacaaccac tttggagaac atgctctatt caaaggaaat
    24601 catggatcag caaatgaaag ggccataccg cgcgatcttc agttccaaca attaccacat
    24661 ttttagagcc ggtctttatg cgcgacaagc caaattgaaa gcaaatggta ttggtgcaaa
    24721 aacagctttc tactacttgc ctaatgcgtt ttgagagaat acatcgccat cttgatgatg
    24781 aacaaaaaaa gacatatgat catttgcgga ttgcttttcg caggctccgc attcttatcg
    24841 ctgattactt tacttttcta aaaaaacaag gtgtgacaaa acatatgtca caccttgttt
    24901 ttttctttca ttctcttaac tattggcaaa aagagtatcc cttttttgac cgctacttgc
    24961 tgagcagaat aaataccgct gttgcctgaa cacataaagc tgatcaagca gatcataaag
    25021 aagtatactg ccgcttgtga accgaacaat tcgatcccca tcacaaagca ggcaatcggt
    25081 gtatttgtcg caccagaaaa tactccgata aagcccaatc ctgcaagaaa aggaacagaa
    25141 acatggagca aaggcgctag tgctgcacca agcgttgcac caatttcaaa caatggtgtc
    25201 acttctcccc cttgatatcc cgcgcctaaa gacaaaactg tgaagaataa cttcccaata
    25261 aaatcataga catgtgcctg tccattaaat gattcttgaa gtaacggaag actcaatccg
    25321 agataacgtt gtgtttgcaa aatcaatgct gctgccacaa ctacagctcc acctatcgta
    25381 tttcgccaaa caggatgagg aatccattgt gcataccgtg cttttaaaaa gacgatcgaa
    25441 cggctgaata cccagccaat caaaccaaaa gcaatcccag caacaaaaac tttgaagaac
    25501 agcaacatac tccattcagg gatcacaccc atttggtaat gtaggtgaga aacaccatag
    25561 ctttctgtca taaaatttgc aaacagtgca gcaaagaagc tagggaagat cgcttctgtt
    25621 cggactttcc cgattgccaa cacttctaat ccaaataatg ttccagctaa aggtgtgcca
    25681 aatacagaac taaaacccgc actgattcca ctgataatca agatttcccg ttctgcttta
    25741 tccaatcgga acaaacgtgc tgtattatct gctaacgctc cccccatttg gactgctgtt
    25801 ccttcacggc caacagatcc cccaaataaa tgggtagtta tggttccaaa cagtgtaagt
    25861 ggaataagtc gcaacgggat tttttcttct ccaccatttc cttgatcgat cactaagttg
    25921 tttccacgac tagaaagacc gccgtgatac gcatagagat aagcaaaaag cgcaccacta
    25981 actggtagta gataaaggag ccaaggatgt gatagtcgga tatctgtcac aactgtcagg
    26041 ctatttaaga aaaaagcaga caagctcccc atgaatatac cgagaatcgc tgtgatcacc
    26101 agccacttca gcatgtacac taaaatgcgt actgtactcg gtaattgttt ttccataaaa
    26161 aattcctcct caataaaaaa acgcctacta aagaaagggg ctgtccccag actttagtag
    26221 gcgtcattag ctatctttcg ttagcattta aaggcgaaca ccatcaccta ttcacttcca
    26281 ttaaaagata gcatattttt ctgtaaaaaa caataaaatt atcgttcttt ctttacacgt
    26341 tcagcaaatt catagaaagg agccaatttc gttaactgtt gattacattg gtcactatca
    26401 tgacaaatat aatttccctt tttcgtatac gtgccatcag aacctgcttt cgttgtagaa
    26461 agaaaaagcg aaacattcga tgttttctga cagattgcac agattccctt atttatcgtt
    26521 ggtgataaag taccgctaat cccttttaat ttttcatctt cgtaatagac catgaatttt
    26581 ttctgcgtac ctggatcatt ccagccgatg taagtatgtt cttgcaagtc gatttcattc
    26641 caagctggtt gtttgagttt tttcactttt cggaataatt tctcgatttg tggtttgcta
    26701 ggctgcataa acggctgaac gatttgcttc agctcctcca atgctctttc tgcttgcgct
    26761 tttgtcagtt ttacgtctaa aactgtcgtt aaaaatgtat cgatttctgg cagttcgtga
    26821 tctaatatgt ctcgaatctt ttcagctgct agtgcctgaa ctgtttgtac cgtttttgga
    26881 tcattgacag atgagtacgc atttaataac tgttctactt ctctttttat aaaaaaatat
    26941 tggtatggtt ttattgtttg ttccatgatt ttaaagtcct ttcttctaac caaagtgcat
    27001 actttgtaga aaaaagtgat cttctgcaaa gtatgtgtta cctgaagatc ccggactctt
    27061 ttaaataaga tacgttttgc atcttatcat cacccccttt aatgcttatt attctatgta
    27121 catgattcat tcgataaata aaagtaaacc gtatcttgaa cagatagtca actgcaaaca
    27181 gaaaaaagga ctgtgacaaa agtcgtgtca aagtcccttg tttcgaatga atggtgttca
    27241 aaagcttgca tctgcggtaa taagccgaaa cttcacaaaa atttgagaag taattttcgt
    27301 aaattccttc ttatttcttg aagctgacca cttctgtcac aacctcaata tggtctatta
    27361 ttggttttac ctgttaaatc gaggattctt tcccaaagta acgttgccat ctcttacgca
    27421 tacttgcaga catgggctga gccgcttctt tgactgcgca gtatttgtcc acgaatgtca
    27481 caatatatcc ttctttatac ttaggcggtg cgatcgttgc accccacatg tgcttcaaaa
    27541 tgatatctcg ctcaagatcc gacaaatcgg tgattttttc tgcattcttt acagcaattc
    27601 ttggatggat atacgcatgt gtcccctcgt caaatttcgt cgtacgccaa tcatagtaga
    27661 acagatcatg caaaagtcca gcacgcgctg ttgctttagc attgccaccc catttttttg
    27721 cgagcagata actatagtaa gaaacactga tggaatgctc caaacgagtg gaatgaacat
    27781 gctgtgtgta atttgctaat tttttcactg cttctgtttc taaaagatct tgtacataag
    27841 acatatattc ttcgtcttca cgccattttt ctgtcataat tatcgatctg ccaccttttt
    27901 gaatttcatt ttcttctgaa attgatctaa gtataccacc agtgttcgga ttattctagt
    27961 cgctctagat aaaatagcta aagcttaata gttccttaat cataaagaaa aatgataaag
    28021 aacgatccct gctgcgactc cgacattcaa tgattccgca tttcctttca ttgggatata
    28081 tacgttctga tcagtcaaag acaagatttc ctgacttact ccctgacctt cgtttcccat
    28141 gattatagcg aaatcctctg ttttttctac tttgtctaat gcatgtgctt cttcatttaa
    28201 ttctgtaccg tacaccgtaa accctttgcc tttcattaca gggatcactt ctgccaaatc
    28261 tttttgtaac acaggcaaat gataattgct tccctgcatg cttcgcagca cctttgtact
    28321 ataaatatct gccgttcctt tacctaataa aacaccagcc ataccaaacg catctgccgt
    28381 acggatcatc gttcccacat ttccaggatc ctgtacatta tctagtaaaa gccagcctcc
    28441 tgaaaaatct ggcgtatctt tagatgttaa cgtcatcact gctaagatcc cttgcggtgt
    28501 aggtagatct gacatagcgg tcatgacctc atctgaaact agaaaagtag aaatctcttt
    28561 gtttacagcc agccagtctc cccattcttt catacctctc tgtgtcacaa aaagttcttt
    28621 tatttctaca tcagacgacg cagcttcttc gatcagatgg aacccttcca aaagatattc
    28681 tgccttttct tctctgtatt tctttttatg tagttttttt aattctttga ttcttggatt
    28741 ttttgctgat tggatctctt tcacattcct tcacctcacc tgattatatc atggaatagg
    28801 tgcttgagct tgtttcatcc taaaaaaatc ggtatcattg atttataaaa caggaaagaa
    28861 aggtgacgaa catgcgcaaa gttaaaatga atgtccaagg acgtgtccag ggcgtgggat
    28921 ttcgctatat gaccaagatg gttgctgatc agttaggcgt gaccggaacg gtaaaaaacg
    28981 aagacgacgg ctcagtcaca atcgaagcaa tcggaaatga cgacatcatc caaaaattta
    29041 tcgaagaagt caagaagtct ccgagcccaa gtggacgagt gcagtatgtg gatgtccaag
    29101 aggatccgat gattgaggaa cgaaagaaat ttgatgtggt gggataaggt tgtgaaaagg
    29161 tcgctgagtt ttgagtatta aggaaaattt tgtgaaaata gctttccaca tttttgcata
    29221 aatttgactt aataccgaga aactgacttt tgaacaccgt cgatcaggtt gcgaaaaagc
    29281 tgatctgcat caagcattaa ggactggttg tgaaaaagtc gcttagtttt gagtattaag
    29341 aaaaattttg tgaaaatggc tttccacatt tttgcataaa tttgacttaa taccaagaaa
    29401 ttgacttttg agcaccgtcg atcaggttgt ttttgaggcg ctttgcctca agtattaaag
    29461 ataggtagta aaaaagctga tctataactt gcaataaatt tgcttaaccc atattttcca
    29521 gacagaacaa aaaagttttt agttttttaa ataagaaaga aacacttgga atcactcttt
    29581 tctttccaag tatttctttc ttgatttttt gtcttttttc aaaatttttc gctacgaaaa
    29641 agaaagaata tttatatgaa atttctatga agttgattga attccatgcg cttttttagt
    29701 atagttaagt ttgtgtaaaa attatagaag aggaagtatt ctatgaataa atggaaaaac
    29761 tggttattgg gatccggact tgtcagtatc ctgctctttt tatctggctg tgtgcggatg
    29821 gattcaaacg gaaaccctga cacttcagga atcgtttatc gagtactcgt ccatcctatg
    29881 ggccaagcga ttacatatct agttgagaat ttcaattggt cttacggctg ggcagttatt
    29941 gcaatgactg tgatcgttcg tatcatcatc ttgccactag gtatcagtca atcgaaaaaa
    30001 acaatgatcc aatctgaaaa aatgcaagcg ttaaaaccac aagttgaagc agcacagcaa
    30061 aagctaaaag ctgcaacaac acgtgaagaa caaatggcag cacaagcaga aatgcagcaa
    30121 gtatatcgcg aaaacggatt gagtatgacc ggcggaatcg gctgtctgcc tctattgatc
    30181 caaatgccga tcttctctgc cttgtatttt acggctcgct acacagaagg tatccgtgaa
    30241 tcatcttttt acggcatcga tctaggctca ccaagtatgg tgcttgtggc catcgcaggg
    30301 gtcgcttatc tgcttcaagg atatatttca acaatcggta ttcctgaaga acagaaaaag
    30361 acgatgcgta cgatgctgat cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg
    30421 ccagccggtg ttacacttta ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc
    30481 atcacaaacg ttatgatgaa accacgtttg aaagcacaag tggcagaaga gttgaaacaa
    30541 aacccgccaa aacaagtcgt cactccaaga aaagacgtga cagaatcagc aaagccagct
    30601 gacaacaaac aagcaacaaa acaaatcaaa caaccaaaaa acagtacaaa taacaaaggc
    30661 cgcaacgcag gaaagcagca gaagcggaag tagatagtaa ataagagtgt aataaaaatt
    30721 cattaacagt tttagtaatt aaaaatatcg accaagattt ttgaaaaaga aatctggtcg
    30781 atatttttat ttaagtgaat gtttaatttg aatcatcgat tgatcgctat ctaattgcag
    30841 cagcatcttt ttatgaagaa acaacttttt cttatctatc aacgtagaca ggtgttgctg
    30901 tttcttctaa tagtgtactt aattctttaa tattttgttt tccttcttct aacatccaac
    30961 tactcgcttt ttcttcgcct tcggtaacaa aaggtgctac tcccttcgcc catgttgctc
    31021 gtccacataa gacaccattg aatgtcgaac ctgattgttt tgcaaaacgc aatgtatctt
    31081 gaaatagtgc agcgctgact cccgcactaa aaaaaataaa cggttgttca gttactttcg
    31141 attgttcata gaaataagtg gatgcctctg cttgactata acaagtctct tgcccatatc
    31201 ctttcacaaa tctcatattc actggtactt ctactttcaa tacatccact tgataacgtt
    31261 cttttgaaaa ttctcgcatg gcttcaatca ctttatgcgg ttttactttt gcataggcaa
    31321 gagaagacgg atcgctaata gttgcatcat aagaaaggat ctctaaaaag aaaggcatac
    31381 cttctgcttc gcattctgaa tcaatccgtt ctataactat ttgtttttgc acattaatct
    31441 ctactgattc atcaacgtca tagtacaata aaaatttaca agcatccgca cccacttctt
    31501 ttaacctttt cactgaccaa ctattcagta aatctggtag tcggcccggc attttcgcat
    31561 catagccggt tttttcataa gctagcaaca agccacattc tttctctcga agcgctgctg
    31621 caggcagacc atattctgga tcgagtaaaa tagcggaagc atacggagtc aggtactgag
    31681 aaaccatctg tttaaattgg atgatatctt ctgatgtagc ttctttatat tggttgatca
    31741 tttttttcaa agcaccacgc tggtcgattg ctaacgcgct aataattcca gcttcatccg
    31801 tcaatcgctt caaacattcc tgtttttcta tactcatttt cttcatatat cttcacctat
    31861 ttctttagtt aatattgtgg ctatttctgt ttttgatttt gctgctctca actgttctgt
    31921 aacctctttt cgcatcaatg atcgtgcaac gagagacaat aatttcaaat gagtcgttcc
    31981 tgcttctgaa tctgggataa ataacgcaaa aataaaatca actgactgtc tatctaaact
    32041 gttccactca atccctttgt tcagtgtgac gataatcaaa ctaggttcgt tgattgtttg
    32101 agacttcgca tgagggatag caaaacctga cattattcct gtagtacctt ctttttctct
    32161 ttctagaagt tttcgatata cttctttttc gtcatgagcg attcctaaac agaatgtttg
    32221 ctttgctaaa aacttaaatg cttcttcttg actatctaat ggctgttgta aaaaaatgtt
    32281 ctcttcatga atgattgtca tgataagcct cctgctcttc cttcttcgat aactgtacga
    32341 tttgctgctt tattatccat cttcttgtct ttatcagtta ctcttttctt ttggatcaaa
    32401 cctagtatca ctccaccaac gatcgaacca ataagaatcg ctgtaaccca ttggattttt
    32461 cccgtcacta caggtaacac taaaaaaccg ccatgtgggg ctggtacttg gacaccaaac
    32521 agataggtca agacagcagc aattgatgag cctaacatca tcgttggtaa tactcggatc
    32581 gggtctttcg cagcaaacgg aatagctccc tccgtaatat gagttgatcc taaaataaaa
    32641 ttaaccaaac ctgcattacg atcattagta tcaaaatatt taccaaacaa taaagttgca
    32701 aaaccagtaa tcaacggtgg tgcaatgcaa gctactgaca cgccagccat aaaagtcgtg
    32761 tttccttgtg ccaaaagagc tgtacctgtt acatatgccg ccttattaat cggtcctccc
    32821 atatcaaacg cacacatgca accaacaatc acgccaagaa caagtggatt cgaatgttca
    32881 aatccagcta aaaagctcat cattccttca tttatagcct tcattggacc cgccagcaaa
    32941 gacatcacga tgccgataaa cactacacca ataactggat ataagaagat tgccttcaag
    33001 ccatctagtt gtttaggtaa aattgtcagt agcttttgca aaaataagat aaaataacct
    33061 gctaagaacc cagctaaaat acctcctaaa aatccagtac cgccactcat ggccacgatt
    33121 ccaccaacaa atccaacgac taaccctgga cgtttcgcga tcccttctgc aatataagct
    33181 gctaaaactg gaaccatcat ccccattgct gcatcgccaa ccgtttttaa catcgccgct
    33241 gtagcattgt attgtgcatg gtctggatca aaagaataga ttccccaaac ggcaaatgaa
    33301 atagcaatca atactccgcc agctaccacg aaaggcaaca tatgagagac gccattcatt
    33361 aaatttttat agatgctgcg accaaaatta gcaccactta tcgtttctga ttctaaagta
    33421 tcaacagctt cattatctgc taagatggtc ccttttcctg ccaatgcttc gtcaatcagc
    33481 cgttgtgcat ccttgattcc tgtactgaca gaaacatcaa tgactcgttt tcccgcaaat
    33541 ctttctgaat ggacatcttt gtctgcggca ataatgacag catctgcctg tttgatttct
    33601 tcagccgtta attcattctc gatcccaatt tgtccatggg tctccacttt gatcgtgatg
    33661 cctttttttc tagctgcttg ctctaatccc tcttgcgcca tgtaggtgtg tgcaattcct
    33721 gtcggacaac cggttgctgc aattagttga tattttccca tctttcgaac ctctcttcct
    33781 tgattttgat ttgtttactt aattcctcca catccaaaaa atcagtcagt cctttacgaa
    33841 aagcagtaga gctgcctgct gctatgcttt tacgtaacgt ttcatcaagc ggtcgtcgat
    33901 tcatgtatcc tactaaaaaa gtgcctaaca tggcatcacc tgcacatgct gtattgacaa
    33961 cttttccttt tggcgagttt cctcgaatca ccctgtcttt cgtaaaaaga atcgctcctt
    34021 tctcaccaag ggataacaaa acattttctg cacctgactc taccagtcgt tgtccataaa
    34081 caaagtaatc ttcatctgta accatttttc gtccgaacca agaagaaagt tcttcctcat
    34141 tcggtttcag caaaaatggt cgatagggca aacaacccat gacctcttga tcactgctat
    34201 caataatcaa atgaatctcc ttttcctgac aaatccgact gatttccact aaaaccttcg
    34261 gagacattcc tcttggtaaa ctcccagaaa cacaaagata atcctctgct tgtaatgctc
    34321 ttatttgatt taaaaaatta tggacagctg cctgcggtat ttcaggtcct tgattcacta
    34381 atttgtattc ttctctcgtt tgattgactt gtgtaaatac attgatacgc gtcataccag
    34441 gcacttcaat aaactctgtt tggatttgtt tcttctgtaa atagtcttct atatattttc
    34501 ctgtgaaacc agcagaaaaa cctaatgccg tacttgggaa atctagcatc tttaatacta
    34561 gtgacacatt tacccctttt ccgtttgcct gaatgtcatc ctcctttgta cggtttacca
    34621 taaaaggatg catctcctct gtttcaataa atagatcaat tgccaaattc atcgtgcatg
    34681 tgtagataga cataaccaat gacctccttg aatctattat gaaatacgcg aaagagaaaa
    34741 tgttttctta atcgttccaa cacaaagaaa acctttacat ttataaacac atcgtgctat
    34801 tttttaacta acttttagtg aaagaaggaa agaatatgga tcacaaatta agtgaaacag
    34861 aacagtacct ctggaacttt atcgaacacc acatcctaga gatccctaac tactctatcg
    34921 tgaaactaag tgaacaagca aatgtctcaa cagccaccat tgtgcggaca atgaagaaaa
    34981 aaggctatga aggatttact tcgtttaagc accatctaaa agaaggagaa aacaagaaca
    35041 tcaatttttc tttcttagat aaagtagaca aaggtatccg tcgagccatt ctaaaaaatg
    35101 aacaagaagt ggtacgtacg atcaatatgt tggaaatcgg gaacattgaa gatgcaatac
    35161 aaagaatcaa atttactgac cgggtttttg tctttgcccg aggattttca gaaatgattg
    35221 gtcaagagat gctcgtaaaa cttcaattaa caggaaaaaa ttgtcaaatg catactgatc
    35281 cagagattat caaaagcatc agtcggaaac taactaagaa agacagcgtt atttttgttt
    35341 ctttgaacgg cgagacaaaa gaactcgtca ccgccgcaaa gaactgctat gatgcagaga
    35401 ttggttcgat tctagttaca gccaatcatt cctctacact caaagattat tgcgagataa
    35461 atctcgtagg tttcaaatca gaaggttctt atttccctga ttatgaagta cgttcacgct
    35521 tgcctctcca gatcatcgcg agaatcctgc ttgacgccta tgtattgcgt atgagggaga
    35581 attgagaagc agtttattat ggttggttac agtttaaaac atcacatttt ttattcttta
    35641 tattgtatgg actcacgtaa actttttatg tataaagtta ttca
//
LOCUS       DACECY010000024        35387 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.025, whole genome shotgun sequence.
ACCESSION   DACECY010000024 DACECY010000000
VERSION     DACECY010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.025"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(97..1116)
                     /locus_tag="IWO38_001268"
     CDS             complement(97..1116)
                     /locus_tag="IWO38_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0313365.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1138..2145)
                     /locus_tag="IWO38_001269"
     CDS             complement(1138..2145)
                     /locus_tag="IWO38_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0313366.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2138..2803)
                     /gene="pgmB"
                     /locus_tag="IWO38_001270"
     CDS             complement(2138..2803)
                     /gene="pgmB"
                     /locus_tag="IWO38_001270"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ0313367.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(2796..5090)
                     /locus_tag="IWO38_001271"
     CDS             complement(2796..5090)
                     /locus_tag="IWO38_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ0313368.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5350..7515
                     /locus_tag="IWO38_001272"
     CDS             5350..7515
                     /locus_tag="IWO38_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0313369.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7819..8652
                     /locus_tag="IWO38_001273"
     CDS             7819..8652
                     /locus_tag="IWO38_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ0313370.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8754..10454)
                     /locus_tag="IWO38_001274"
     CDS             complement(8754..10454)
                     /locus_tag="IWO38_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313371.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            10961..12121
                     /locus_tag="IWO38_001275"
     CDS             10961..12121
                     /locus_tag="IWO38_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0313372.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12137..12580
                     /locus_tag="IWO38_001276"
     CDS             12137..12580
                     /locus_tag="IWO38_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0313373.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(13063..13593)
                     /locus_tag="IWO38_001277"
     CDS             complement(13063..13593)
                     /locus_tag="IWO38_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ0313374.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13698..14249
                     /locus_tag="IWO38_001278"
     CDS             13698..14249
                     /locus_tag="IWO38_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0313375.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14402..14875)
                     /locus_tag="IWO38_001279"
     CDS             complement(14402..14875)
                     /locus_tag="IWO38_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0313376.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            15013..16485
                     /locus_tag="IWO38_001280"
     CDS             15013..16485
                     /locus_tag="IWO38_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0313377.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16482..17192
                     /locus_tag="IWO38_001281"
     CDS             16482..17192
                     /locus_tag="IWO38_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ0313378.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            17544..17828
                     /locus_tag="IWO38_001282"
     CDS             17544..17828
                     /locus_tag="IWO38_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ0313379.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(18109..18528)
                     /locus_tag="IWO38_001283"
     CDS             complement(18109..18528)
                     /locus_tag="IWO38_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ0313380.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(18530..19114)
                     /locus_tag="IWO38_001284"
     CDS             complement(18530..19114)
                     /locus_tag="IWO38_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ0313381.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(19161..20636)
                     /locus_tag="IWO38_001285"
     CDS             complement(19161..20636)
                     /locus_tag="IWO38_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ0313382.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            20817..21128
                     /locus_tag="IWO38_001286"
     CDS             20817..21128
                     /locus_tag="IWO38_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313383.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(21243..23579)
                     /locus_tag="IWO38_001287"
     CDS             complement(21243..23579)
                     /locus_tag="IWO38_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0313384.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            23721..24389
                     /locus_tag="IWO38_001288"
     CDS             23721..24389
                     /locus_tag="IWO38_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0313385.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            24392..25435
                     /locus_tag="IWO38_001289"
     CDS             24392..25435
                     /locus_tag="IWO38_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0313386.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(25672..26553)
                     /locus_tag="IWO38_001290"
     CDS             complement(25672..26553)
                     /locus_tag="IWO38_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0313387.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            26712..27440
                     /locus_tag="IWO38_001291"
     CDS             26712..27440
                     /locus_tag="IWO38_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ0313388.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            27596..27913
                     /locus_tag="IWO38_001292"
     CDS             27596..27913
                     /locus_tag="IWO38_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0313389.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            27906..28514
                     /locus_tag="IWO38_001293"
     CDS             27906..28514
                     /locus_tag="IWO38_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ0313390.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            28530..29360
                     /locus_tag="IWO38_001294"
     CDS             28530..29360
                     /locus_tag="IWO38_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ0313391.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            29498..30010
                     /locus_tag="IWO38_001295"
     CDS             29498..30010
                     /locus_tag="IWO38_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ0313392.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            30036..30908
                     /locus_tag="IWO38_001296"
     CDS             30036..30908
                     /locus_tag="IWO38_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ0313393.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            31084..31446
                     /locus_tag="IWO38_001297"
     CDS             31084..31446
                     /locus_tag="IWO38_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0313394.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            31455..32207
                     /locus_tag="IWO38_001298"
     CDS             31455..32207
                     /locus_tag="IWO38_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0313395.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVKPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            32221..34023
                     /locus_tag="IWO38_001299"
     CDS             32221..34023
                     /locus_tag="IWO38_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0313396.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAEKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            34300..35340
                     /locus_tag="IWO38_001300"
     CDS             34300..35340
                     /locus_tag="IWO38_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0313397.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT        11054 a         6255 c         7288 g        10790 t
ORIGIN      
        1 tttttacaga taaacaatgt gttagaagta gtatcttctt ttacataaaa agctgaccat
       61 aggataaatg gtcagcgtat ttagtgagtc agctatttag tatgggtttt ctaaaggaac
      121 gactgtctct cttttgatca agcgatgagg gatgaccaaa cgtacgccac tgctcaattc
      181 ttcatggatc aattcgcgca gcttttgcat agcgacacgt cctaattcgg agatatcgat
      241 atcgatactt gtaaggtaag ggtgtgttaa tgttgagaag atcgagttgt tgaagctaat
      301 gacggagagt gtctctggaa tgacatagcc atacatttgc gccaactgca tcgtgcgaag
      361 ggcgaagata tcgtcaatga ccaccaatgc tgtggcttcg ttttctttca acgttttctc
      421 aaaggacaga taatcctccg gatgctctag tactacactg gataacgcag gaagaccagc
      481 catcatcatt gctttttgat aaccgaaata tcgttcgaaa tacaaacttt catgagtcgt
      541 gtttgtcaca aacaggatat tcttatgtcc attctggatc aaaaattctg ttgcttgttt
      601 acctagaagc tggttatcat tatctacata aacgatttcg ttctcatgat gataaggctg
      661 tccgatcaaa gtgaaaggga tgtggttttg gtataaataa tcaatgacag tatcttgact
      721 atctgaatac actagaataa aaccatctac ttgtttttgt aaatgcatac gctggacgtt
      781 ctctaacagc aattcaaaat ttttagcagt agcgattgct gtagtcatat cataattccg
      841 cgcttcttcg ttgattgctt ccatgatctc aagataaaat gggttaccga tccgctcttt
      901 tgaatcaaga gggggtaaga ttacgccgat ggcactggaa atgcgttttc ctagattgcg
      961 ggcagtcatg ttaggaacat aacctagttc atccataatt tttcggactt tcttctttgt
     1021 tgtctcggaa atactaggat gatcgttgat gacacgagag acggtcgagg tggcaacacc
     1081 ggcttttttc gccacatctt ttacggtaat agccatgata aaaacacctt tctcttgtta
     1141 ttgtgcagag aaccgataaa tcgtctgact gatccgttct tgaccaggat ggaggttgat
     1201 cgatccaaat ttggggaaat gaacgatatc agggatttct tgcggttcga ttgctaatcc
     1261 gtagtttgaa tgcatctgtt ttccattgac agagaaatca gcttcgaatc cagtcgtaga
     1321 aaataatacc atacttttat tagaagttgc tatagtcatt tggcgaccag aatgcttctc
     1381 gaataattgt aaggaagttt tggaaagcct aggatgatgc aaaagaaata catcatctag
     1441 tccatctgga taacatgcaa ggatctcttg cagtgttggg gattttctaa agtcgattgg
     1501 cagtttttct ttttttgtcc aatttccagt aggcagtttt tcttgatcta attcaagcac
     1561 accatctaaa aagaccgtta actgatgttc atagatgtct cttgttccat ttcctgaaag
     1621 gttgaagtag gcatggttgg tcggattgca gatggtttca acaaggctac tagcagaggt
     1681 tgtcatctct aattcgtttt ttgttaaacg gtaagtgatc gtagcagtga ttggtcctgg
     1741 ataggtagaa aattcatctt ttaagtaaaa gaccacacct attgatgatg gagttttgaa
     1801 aacttctact tcccaaaatt ggaaagacca gcctttcgtg ccaccgtgga tatggtgtga
     1861 tccgcagttt ttctccaatc tatgctcacc ccaagaagca tcccggatcc ttccggctac
     1921 aggtccgaca gttgctccaa aaaagctttt gtctgttaga acatcttcga aacgatcgta
     1981 agacaataaa acattttctg attttccttc tttgtctggt gcgaagattt ggtgcaggcg
     2041 ggcaccgtaa ttgagaagaa cgacttctaa gtagtcgttc ttcaatgtga ttttctttat
     2101 ttcagtctct ggaactggta cgacggagat ctctggatta tccatggtca tgccactttt
     2161 ctttcaagta atcgatcgta agaaaagtcg tatcagggac aagcgggatc tcctctccaa
     2221 ggtcttcagc acgtcccacg ccaattggtt gagcgccact tgagaggatg gcttggatac
     2281 cggcttgcgc gtcttcgatc ccgatacatt cttctgcttt tagtccgaca gcttttgctg
     2341 caaggaggaa gatatctggc gcaggttttc cgtttgttgc ttctgccggg tcggcaatag
     2401 catcaaagta aggtgtaaga cccatttttt caagtaggaa tggcccgttt ttacttgcag
     2461 aagcaagagc aatcttgatt ttttcttctt ttaaagaatc cagtaaaggc acgaccccgg
     2521 ggaagacatc ttttggttcg atcgtctgga tcatctctaa ataataatcg ttttttcttt
     2581 tcgctaattc tgcaaattct tcctttgaaa aagagtgttc ttttccgcca taagctaaaa
     2641 gcaaagagag tgagtcttct cggctgacgc cttttagctg ttcgttgaat gtgtggtcga
     2701 tcgtaatgcc gatttcctga ccaagctttc gccaagcacg ataatggaat tcagcagtat
     2761 cggtaatgac accgtctaaa tcaaacaata cccctttaaa catttgcttc ctcctttctt
     2821 agcgttattt ctagacgatc cgctaaagaa taagactgtt catagacgta aatttctaga
     2881 ggagaacctt ctaataactc gaaagtaact tgttcatctg tgactgagat gaacaataaa
     2941 cgtccgcgat aattgatatg gaaagaataa gctttccaag tttttggcag aaatggtgcg
     3001 aatctcaact gatcatgggc agttttcatt tgagcaaatc cttggacaat cgacaaccag
     3061 ctaccagtca tggaagtgat gtgcaagccg tcttctgtgt cgttattgta attatccaaa
     3121 tccaagcgag ctgttcgctg gtacatttct acagctttgc tttccattcc taattctgct
     3181 gctaaaatcg aatggatact tggcgaaagg gaagactcat ggacagtcat tggttcatag
     3241 aaatcaaagt ttttttgctt ttcttctttt gtaaaagtat cattgaagaa ataaatcccc
     3301 tgtagtacat ctgcctgctt gatgaagcaa gatctaagga ttttgtccca agaccaatgc
     3361 tgattcagag gaagatcgct tgtgggaata tcggatacag gcatgagatc tttatctaag
     3421 aatgtgtcat gctggacaaa gattcctaat tcttcgtcaa ctgggaagta catgttctct
     3481 gcgatatctt tccatttggc tagttcttct tcgctgatag tgatagcagc ttctttttga
     3541 taagttaaat aattttctcg cgtgtaacgc aagacccaag ctgcgatcgt atttgtatac
     3601 caattgttat tgatattgtt ctcatattcg tttgggccgg tcacaccgtg gatcatgtat
     3661 ttatttgctc gttttgaaaa atggacacgg tctgcccaga agcgtgcgat ttctgctaaa
     3721 acttccagtc cttcattttt caaatattcc gtatcgccgg tgtaattcgt ataattgtag
     3781 attgcatagg caatcgcgcc atttcggtga atctcctcga atgtgatttc ccactcgtta
     3841 tggcattcga caccagtgaa ggtgaccata ggataaagcg cgccttttaa tccttgttgc
     3901 tgagcgttgt ggattgcttg cggcagttga ttatgccgat atttcagcag attcttagtt
     3961 acttcaggtt tggctaaagc aagataaaga gggacagcat acgcttctgt atcccagtaa
     4021 gtggctccgc cgtatttttc tcctgtgaat cctttggggc cgatattcaa tcgttcatct
     4081 tcgccgtagt aagtagagaa tagttggaac aaattgaatc gaataccttg ttgtgcttct
     4141 tcgtctcctt cgatgaccac atcggcaagc ttccatcgtt ctgcccaagc cgctgtttgc
     4201 tcttcttttg cttggatata attgtttgtc tggttcgtta attctgagac agctttttct
     4261 gcttgctgcg cttctggaat atctctacta gtaagaacga taatttcttt ttccactgac
     4321 catgtctcac cttgagataa ttctgcagac aagcaaccat gtaatgctaa agtatcttcg
     4381 tgatagtcag ggatgactga ttttccatta acgaaatggc gcattgctgc agtaacagta
     4441 aattgttcaa tcccaaagtt gtttggaata gttttagtcg tcaaatagcc gatttcttct
     4501 atagttccac gatcaatctc attccaaaaa tgttcatcat agttgctgtc ttcgttttga
     4561 acatttccat ccaattttga tacgagtttg actgttccgc taccttcaag catttctata
     4621 gttaaataga tataagctga ttcttttttg acaatgctca gaaaacgttc gaaggaaatg
     4681 cgtactttat tagtagaagt agtgattgtg aatgtacgag ataatacacc atttttcata
     4741 tctaattccc agtaaaagtc ttctggttcc aaagaagcca agtcgatcag ctgatcgttg
     4801 atcgaaatat ctatcgcgat aaagttcaag gcgttgatca cttttccaaa gtattctgga
     4861 taaccatttt tccaccagcc gacacgtgtc ttatcgggat accagacacc cgccaaataa
     4921 gtaccttgat ggtggtcccc tgagtaagtt tcttcaaagt tcccgcgcat ccccatataa
     4981 ccgtttccaa tacttgttaa agattcttgc agtcgaagat tttttttatc taattgtgtc
     5041 gtgctgattt tccatggatc aatagcaaat agtcgtttga tctgtttcat ttttctcctc
     5101 ctaatgagct gttttatacg ttcataataa acgcaaccga ttgcacaagt caatagacaa
     5161 aagaaaatat ttcatagaaa aaagcttaaa aaaataaatt tttaggagaa aattatagaa
     5221 gggaacacac gtattatagg ttttatagaa tggtatagac agaaaaataa aaaataaagc
     5281 atttacaaaa aagaaaacgc acgctataat acgtttaacg aaaccgattg cgtaaaggag
     5341 ctaaacggaa tgaaaaagat atttagtttt gaattttggc aaaaatttgg aaaggcttta
     5401 atggttgtgg tagcagttat gccggcagct gggttgatga tcagtatagg gaaatcaatt
     5461 cccttgatca atcctgattg gacgccgctt gtcacaacgg gcggagtaat tgaaaatatt
     5521 ggttgggcaa ttattggtaa tcttcatatt ttatttgctt tagcaatagg aggaagttgg
     5581 gcaaaagaaa gagcaggcgg agcatttgca gctgggatct catttatttt aatcaatcgg
     5641 atcactgggg ctatctttgg tgtcacaagt gacatgcttg cgaatgaaga tgcatttacc
     5701 cacactctgt tcggtacgaa gatcatggtt aaaggatttt ttacaagtgt attggaagca
     5761 ccagctttga atatgggagt attcgtaggg atcatcgctg gatttgtcgg ggcaatggct
     5821 tataataaat actataatta tagaaaattg ccggatgctt tgtcattttt caatggaaag
     5881 cgctttgttc cgtttgttgt tatcttatgg tcaacaatcg tggcattgat tcttgctgtg
     5941 gtgtggccaa acgtacaggc tggaatcaat aattttggtt tatggattgc agaatcacaa
     6001 gaaagcgcac cgattctagc accgttttta tttggaacat tagaacgttt actattgcca
     6061 tttggattgc atcatatgct gacgattcct atcaactata cacagcttgg cggatcgtat
     6121 gagattttat caggcgcaca ggcggggaca caagtatttg ggcaagatcc gttatggttg
     6181 gcttgggcca ctgatttagt gaatcttaaa ggtgcagggg atatgtcgca atatgagttt
     6241 gtgttgcata actggacgcc ggctcgtttc aaagtaggac aaatgattgg ttcttcaggt
     6301 atcttgatgg gaatggcttt tgcgatgtat cgtaatgtcg atcctgataa gaaagcaaga
     6361 tataaatcaa tgtatttttc agcagcgttg gctgtctttc taacaggtgt aacagaacca
     6421 ttggagttca tgtttatgtt tgctgctgtg ccattatatg tcatttattc agtgatccaa
     6481 ggggctgcat ttgcgatggc agatatcttg ccattgcggg tccattcatt tgggaatatc
     6541 gaattgctga ctcggacacc gttagctatc aaagcgggtc taggtggaga tcttatcaac
     6601 ttcgtgttgt gtgtgatcgc ttttggtgtt gttacgtatt tcttagcaaa tttcttgatc
     6661 aaaaaattca actttgctac accgggacgt aacggaaatt acgacaatga cagtgaagaa
     6721 accgttgtat ctaacagcgg aacaggaaca gcagatcagc aagtggtcca gatcattcat
     6781 ttgctgggag gaaaagaaaa tatcaaagat gtcgatgctt gtatgactcg gttacgcgta
     6841 agtgtaaatg atcgagaaaa agtagggact gaagaagcct ggaaacgagc aggggcgatg
     6901 ggattgatcg tcaaagacaa tggagtccaa gcagtttatg gaccaaaagc agatgtcttg
     6961 aaatcagata tcgaagatct gctgcaatca ggagcagaga ttccagagcc agaaatggtt
     7021 tctacaggaa atggacaagc aggtagtaag caatacttag gtatagaaca agaactagtt
     7081 tccgcagctt ctggagaagt gattccttta tctgaagtaa aagatccggt attctcacaa
     7141 aaaatgatgg gtgacggctt tgctgttatt ccaacatcaa gagaagttgt tgcgccgatc
     7201 gctggaaaag tgacaagtat tttccctagc aaacatgcaa ttgggctgga aacgaaagat
     7261 ggtatcgaag ttttgatcca tatgggaatc gatacagtcc aaatgaatca gccagctttt
     7321 gagattgctg tcaaagaagg ccaagaagta ggaacaggaa cgaaattagc tgagatggat
     7381 cttgatgtga tccaaaacga agaaaaagat ccaacgatca tgatcgtatt tacaaacgat
     7441 aaagtagaag aagtagtgat caagcaatta ggtacagtga cagctggtaa agtgatcggc
     7501 tccattaaaa tgtaaggaga ggttgtgaaa aagctgtcct gcatcaagtc ataagaacag
     7561 ccaaacaaaa atagctcctc ctatttttcg tttggttggg cttattaccg agaagcagca
     7621 gtttgaatac tgtttagtgg agaggtcgtg aaaaagctgc cctgcaccaa gtgataaaaa
     7681 cagccaaaca aaaaatagct cctggtccat tgagcttatt actgaggagt tagcaggaga
     7741 acactatttg ctggagaggt tgtgacaaaa attttgtcac aacccttttg tttcgaaatg
     7801 aagacaagga gaaaaccaat gaaattcatg acactcaaca cgcatagctg gcttgagcca
     7861 gaaccagaga aaaaattgca agaattagca gataagatcc tgttagaaga ttatgaagtg
     7921 attgctttac aagaaatcaa ccaattactg gaatctgaag aagtagaacc tgcgaagcta
     7981 atgaagttct gtccggtaaa gaatcaagta cctatccgta aagataattt tgcctaccgt
     8041 ttagttcagc ttttaaagga acacggaaaa gagtattttt ggagttggga aatgagccac
     8101 atcggttatg ataaatatga agaaggaaat gcactgctta cgaaaaaaac attggaaagt
     8161 caggtcctta ccgtctccca atttcaaaag aaagaagatt atcagacacg caagatcttg
     8221 attgggaaaa caaagataga tgatcaggac atttttgtct cgagctgcca cttttcttgg
     8281 tggacagata aaaaaagtgg cttttatttt gagtggaaaa acttggaaaa ctactttttg
     8341 gaaactcgcg taccactttt tttcctaggt gatttcaata atccagtaga ctctcaaggt
     8401 tattatactg taagagaaag ttgccttctt cttcaagatt cgtatgttgt cgcgaatgaa
     8461 aaaggaaaag cagctactgt ggaaaagaaa atagatggtt gggaacaaaa tacagaaaaa
     8521 ttgcgtatag attttatttt cgtccctgaa ggaatgcagg ttaaaaaata tcaacggatt
     8581 tttgatggga tcgatagtcc aatcatcagt gatcactacg gggtggaaat cgaattagat
     8641 gtgaacgaat aaaaaatata acatataaca taaaaaaact gattaaaata aaactctcca
     8701 aacgattata ccgtttggag agttttttga tcggatgtat ttatcccttt tgcctaagca
     8761 cgtaaaagct tgtatctatg ataaataaat aaacatagca aggtaaacca gccaatcaaa
     8821 gtaagagaaa gaatgaaata gaaatagatt ggcgtttgat aatgaagcgt gagctcgttg
     8881 attccttttt gctgcttcac gattggggtc ccgatatctg ttacttcata atcagtcaat
     8941 gtttttccat tttgctgtag cacagtccga tcgtagacga taacagggat atgaacagtt
     9001 ttgtccccag tgtttttcca cttgattgtc aaaagaccgc ctggatgtgt ttttataaaa
     9061 gttgtttcgt tttctagtat ttcttcgtta taccggtcgt atttattgtc ttttgtctcc
     9121 ttatagattg gcagatagtc aggtgtggtt ttttggaaaa cgaacaagct ggtagacata
     9181 tctttgctga agaatgtttt ccttgcttct tccggcgtat cgaatagata agtatgtcga
     9241 ctgacaaatg tttcattttc ccacttttcc agatgctggt ataagttctg gatcgtctgt
     9301 gctgaaaaaa taagaatgcc aaatatactt aaaagacgga accacttttg ttcagaccac
     9361 tggttcagtg aaagggcaag gtaaagtaga agcaatagcg taaatggcac gaaaaatcga
     9421 aatggaaact ggatcaaact aataaaaggc acattttttt cgatcaatgt cgtccatgga
     9481 atcaagctag tagaaagaaa aaagaaaaca agacttgtgt acaacacgat tttttggaat
     9541 aaggtgttct tttgttgaaa gaaaataaaa agcaaaaaac ctgataatat caatatagga
     9601 aaggccttag ggtaatggag ccaataactg ccatccagat ctatcgcctt ttgactcatt
     9661 tctctgttaa caaagggagg aagaatctta ttcccactgt aaacaatagc taatcctgcc
     9721 caaatatttg ctgtcagaac aaaataaagc aagaccgact taatcagttg aagcagtgct
     9781 tttccttttt cagtctggtt aaaaaagtaa taaagataaa aagggatata catcaaagct
     9841 aacatgatcg aagacagtaa atggatctgt gtcataagtc ccactgagac agcgactttg
     9901 ataacaggga actttttatg aaggacgaaa tcagcaatag ggatcataca taaaggataa
     9961 aaacaagtcc cccagctagt gaatccttgg ttgatagacc agtattgaat agaatacgta
    10021 ctcatgaata aaacggctac tggtacactt acccaatttc gaacattgac atatctcagt
    10081 aaaatcagca tagaaaatcc tgaaataaga taaagaatga aatttgaaag aatctgataa
    10141 tgaaaccagc tacctgcaag caacacgatc aatccttgta aataagcgaa aaatgggccg
    10201 taaacggcat tcacgatcct gccactttgc tggaatccat aaagggaagg aaaataatgg
    10261 aaatccccat atttaatttg ttgcgcggca tcatagaacc gattgtaatg gaatggggca
    10321 tcagcaccca aaattagttt atgtgtgaat aaatgaggca tgagaaatgc caaagcaatg
    10381 aaaaaaattg ctaaaaatgg gccatatttt ttcaattgtc gaactccttt gcaaacttta
    10441 gataaattca tcatatatcc tatgcgagag gtaggcaaga aattagaaaa ctcatgaaaa
    10501 tatttatgaa ttttacaaga aaaacataag tgaaagttct aaggagagag gatgcgacat
    10561 aagttttgtt gcatctcctt gttttgaaac agaatttttc ttaatactcg aaacaaagta
    10621 gctttttcat gacctctcgg taaacggtgt tcaatcagct gcttctcggt aataagctca
    10681 ctagcctaga aaatatgaga agctattttc tgactagcgc tcttattact caaagcataa
    10741 cgcagttttc acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag
    10801 cccaacccaa caaaaaatat gagtagctat ttttttgggc tgtacttatt acttgatgca
    10861 ggacagcttt ttcacaacct catcggcttt cattttctat ttttttcctg aacccatata
    10921 ataaaagaaa gaacaaaaaa acttgcaagg gaggacacat gtgaagcaga gtaagcaaaa
    10981 acagtctcgt cgacttttgt ttcgtttgtt tttattaata tgtatcgtcg tagggttact
    11041 gatcgcttta tatccatttt atgtggattc tttaaatagt tttattgatc aaaaacgtat
    11101 cgaacaagta caaaaacgga ccgcagctga aattgaagtg cagcgaaaga aaatggagga
    11161 gaaaaaccaa cgattaactg accaaggatt caatcctggt actgatcctt tcaatgaaaa
    11221 gaacagaaat gaaagtacaa catccagtca attagaggaa tggctgatcg gaagtatcaa
    11281 tatacctaag atccaattaa atatttcact ttatgatcgg ctcaatggta ggatccttga
    11341 aaatggagca ggcgtcttgc aaggaacctc tttcccgctt ggtggaaatt cgacgcattc
    11401 agtgatctct gctcacagcg gtttacctaa tcgacggtta tttaccgaat tagatcgttt
    11461 agaaaacgga gacacgttta ttttgactgt tttgggggag aaattggctt atcaagttaa
    11521 aaatatacaa gtggtactgc cagatgacac gtctgttcta acgatttcag aaggagaaga
    11581 cttagttacc ttgcttacat gtacgccgta tatgatcaac acgcatcgtt tgctagtgac
    11641 aggacatcgg attccttata acgaatcggt aaaaaaagaa gaaggaaaag gaaatcagga
    11701 aagaaccatg cggcaatggt ttattcttgc aggaacgata atagctgtag tcatactgtt
    11761 agtgtttatc ggacgactaa tctatcagta tagattaagt aaaaaagtgc ttgatttttc
    11821 atttattatt agtgattcgt cagggaaacc ggtaaatggt agttcattca ttttgaaaca
    11881 taagaaaaaa acacttacta gaaatggtgt gccgttttca gtacaaagcg accatcatgg
    11941 gaaagtcaag ctggatcaat tgcctggtgg aacttatcga atcgtttcag ctgatgatcc
    12001 taaagttgct gtttcttttg gtattagaaa agtaaaacaa gagaagatat acttttttga
    12061 ggggcgaaaa cttgtaaaag agcttcaaaa aaatggtttt tattttaaaa tcaatgaata
    12121 agtaaggaga gaaaaaatga tccgcacaac aacaaaattg tctgccaaag aactggaatc
    12181 tgttctatcg atttggctgc atgctaactt ggattctcat gaattcattc catcaactta
    12241 ttgggagaat caagtagaat ctgttaggaa agctttacca gatgcagtga tttacttagc
    12301 agaaatagat caagaaatta ttggttttgc tggggtgatt gatcagttta tcgcaggaat
    12361 ctttgtgaaa agggaatacc ggcaacaaag aatcggtacg caactaatga aaaaaataaa
    12421 agaaaccaat aaaacactga ctttgacagt ctatcaaaaa aatcaatcag ctattcattt
    12481 ctatctttca caaggtttta caattcaaac acagcaaaaa gatgaagcag taaatgagga
    12541 ggaacttgtg atgaaatgga atcaaaacgc tgatttttga tgaacagaat aacgaaataa
    12601 tgctcccagc ctaatttttt gctgggagca ttatttcatt ttgaagtcac taatttaact
    12661 gtgtgaatat gcagttgtta caatctttga taaacggtgt tcaatcagct gaccctcggt
    12721 attagctcaa aaaacggcaa aatatgagga actatttttc catttgtttc tctaatactc
    12781 aggtcaaaac gctgatttca caacctcttg ataaacggtg ttcaaacggc tgcttctcgg
    12841 taataagccc actagcctag aaaatatgaa aagctatttt ctaactagcg ttcttattac
    12901 tcaaagcata acgctgattt cacaacctct tgataaacgg tgttcaatca gctgaccctc
    12961 ggtattagct caaaaaacgg caaaacatga ggaactattt ttccgtttct ttctctaata
    13021 ctcaggtcag aacgctgatt tcacaacctc tctttcacaa ccttatttta ctcgtttata
    13081 attttcatcg aagtattttc cgctacggat atcatctggc attccatcgt atggttcttc
    13141 gctaataaca tcttcattgt ctatacctgc atcacccata taagtgatcg tagcaaattc
    13201 aggaacaagt aattttggat aagtttcata tttttcacga gctgcttcca tttctgcgat
    13261 atgttcgttt tggaaagtaa cagtgatttc tggatgttct tcaacccatt tagggaaaaa
    13321 gatcattccg tgaagtttct tttcatttgg cataaagtcc aatgccacgt ctgtccctgt
    13381 tggctctgtc caagtgattt tgaatacgcc gtccgtcaat ttaacgatat cagcttcttg
    13441 gtcttttacc caacggcctt tgaccattcc gccatggata cggtaatcaa cagtatggtc
    13501 atttttggca taccattcat attcccaacc attgtcatac gtatagataa agtgagttcc
    13561 aataaaatct tccaattctt taaattcttt catgtttaag catctcctta taaattaaaa
    13621 catgtaataa actacatgtt ttcgattact catttatgat agaatggatt taaagatttg
    13681 tcaaggaggg aaaactgatg gcaagaaaaa atacacttcg atatattttg ttagggttat
    13741 taagtaagaa gaagatgaca ggatatgaat tgaatcaatc gttcaaaaat gaaataggag
    13801 aattttggca agcaaaacat agtcagattt atcctgaact tgcaaaaatg gaagagcaag
    13861 gaatcattca gcatcaagta gagatcacag gtgaaaaatt ggaaaagaaa gtttatgaat
    13921 taacagaaga agggaaggaa caattggcag agtggattca tacacccacc aatgaactac
    13981 cggttaatcg agacgaattt gttctgaaat tgtatttcgt caaagacgtg aatgatccgg
    14041 cattagtaga aattattcag cagcagcgtg accttcatga agaaaaacga cttcatctat
    14101 tagagcgaaa aaaaacgatc tttcctactg aaaaagaaaa acatgaaaat tacggacact
    14161 acctgatttt agcgcatgcg atcaatcggg aaacagaata tgcaaattgg ctaacagatg
    14221 tattagaaga aagtaaccga aaaaaataaa aaaatatccg aacagtgatg gagaattcct
    14281 ttctatatga aagaaacgca gatcaccgtt cggatattct tatgttcaaa caggcctttt
    14341 ttcaagtatt tatgtagcat ctacttatta aaaagcttgg tacttaggag attagtggcg
    14401 tttagagttc ttttaacaac tgttcgtagt cagctttttc atcgacagga aggagcttgt
    14461 aagttaattc gtctgtctcg ttgtcatgaa tgatgtacat cttagcattc tcttctttga
    14521 agttaccgat ttgtaaaatc gtatagcgtt cgtcgatcaa ttcgatatct ttgattgctt
    14581 ttgaaaaaac tttacggaca tggtgcattt tcttttggcg gatttctgcc ttttctgcag
    14641 cttttgacaa agtaaatccg tcatctaaat attttttgat cagttctact tttatgactg
    14701 taggtaaacg atattttcta ggacaattcg catcagcttc gatcgaacga atgaatccct
    14761 tttgttccca ataccttaat tgacgtggag acacacctac gatatcactt aattcactga
    14821 tgccaacaac caattgatca ttttccaaca aacgtttaag ggaagcccca accatttttg
    14881 tcactccttt tgtcctcttg cttcccttaa ttatatccgt attaacttat gtgtcaagta
    14941 ggttgacaaa tagcgaattt aggactaaag tattctagta gtttcagaaa aaattcagaa
    15001 agaaggagaa atatgagtaa caaccaaccc gtagatattt acggaaaacc atataatcgg
    15061 acacttttag ttgttgtctt attaattggt acattttgta ccgttttgaa tcagacctta
    15121 ttgacgacag cttttcctac attgatgaaa gctttcgata tttcagcgtc tgatgtgcaa
    15181 tggttaacga ctggtttctt gctagtcaac gggatcatga tcccaataac agcatggctg
    15241 atcaataaat tcagttcaag aaatttatat ctttctgcca tgacgatctt tttgatcgga
    15301 acgattactt gctttacagc acctaatttt agtacattac taatcggtcg tttgatccaa
    15361 gcttgcggtg tgggagtatc gatgccatta ttgcaaaata tcatgctatc tattttcccg
    15421 ccagaaaaaa gaggatcagc aatggggatg tcagggatcg ttattggact tgctcctgct
    15481 ttaggcccta cattatctgg ttatatcatt gataactacc attggcgtga cttgtttggt
    15541 atggtactgc caatcgttgt tcttgtcttg gttttagcat tcttcctaat gaaaagtgtt
    15601 attccattgt caaatcctag cattgatatt ttatccgcta ttttatcaac gattggattc
    15661 ggtagtttac tttacggatt ttctagtgta ggagatgacg gctggggaag cttgaaagta
    15721 attggtttct tagttgttgg tgtgattttc atcgccttgt tctcatggcg tcaattacat
    15781 ttggaacatc cattcttgga acttcgtgta ttcaaatcat ctacattcac gattgcagct
    15841 gttttagctg gtgtaacaaa tatggcgatg gtaggggcag aaatggtcgt accattgtat
    15901 attcaaaata ttcgtggaga atcagcattc cattctggct tgatgttgtt gccgggtgca
    15961 ttgatcatgg gggctatgat gcctgtgacg ggtgcgattt tcgataaata cggcgcgaaa
    16021 cgtttagcca tcacagggat gttcattttg acagcggcaa ctgcaccatt tgctttcttg
    16081 acaaaaacga ctccagtttt atatatcgtg atcctttatg cgatccgtat gtttgggatc
    16141 tccatggtca tgatgccggt aacgacttct ggtatgaatg cattgccaaa taacttgatc
    16201 agtcatggga cagcggtaaa taatacattc cgtcaaatcg caagctcgat tggtacagct
    16261 attttgatca gtgtgttgac gaatgtgaca aaagcaaata tcccaagcaa atctgtattg
    16321 gataatacgc cattagctta taaagatcag gcaatcaatg ccacactgtc aggttaccat
    16381 gccgcgttta tcgtagcgat catctttggg attcttggct ttattattgc ctttttattg
    16441 aagaaaaaag aacctgttct aatgaaagcg ggggataatg catgattatt ggagttttga
    16501 ttgtcagctt gcttgctttt gctttaacga acgtgttcgc taaaaaaaca tggcaaacct
    16561 ttttatcttt gattttcgga ctaatcttcg tcgcttcact aagtttgatc gtcgctaatt
    16621 tgtccaatca ctttggaatg gaaaaagtaa cggaaacaaa aacagaaaaa attgtttcca
    16681 gtgctgattc ccaaggagca gatatgcttt tgtataaagc tttagggaat ggaaaagaaa
    16741 aagtgtatct ttaccgaaca aatgaaaaac aagaaaaacc aaaagcaact ggtacggata
    16801 acgaaacaaa taaagtagaa aagacagacg gcgatgctga aaaagtgacg aaaactactt
    16861 attgggaata caaaaacgac atgtataaat tctggttcaa tattgctgat aacaatcacg
    16921 aatatgacag ccgagtgaat acattcaaaa tcccagagac atgggtagaa ttaagtacgg
    16981 accaagcagc caagttagct gaattagtga agaaacagca aagtacgatg gaaagtgaag
    17041 caaaagctta tgttcaagac ggtatggtaa aagcaatgac tgaaaatcca aaaatgagca
    17101 aagcagagca agaacaacgt acgaaagcct tagctgctga attccaacaa caagcattcg
    17161 caaaattagt gaaagaagca aaaggcgaat aatattttga acaccatgta ggaggttgtg
    17221 aaaaagctgc tctgcatcaa gcagtaagaa caatcagata aaaatagcac tccatatttt
    17281 tcatctgatt gggcttagtg ccgtagatgc aagcttttga acaccgttta aggaagaggt
    17341 tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca gcttctcatg
    17401 tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt ttattcgaaa
    17461 aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat gctatactag
    17521 atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca aaaatctatg
    17581 cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata agaaaagaat
    17641 tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat gaaggcagta
    17701 tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt ggactacgaa
    17761 aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt acagtgaagc
    17821 aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac ggtgttcaaa
    17881 agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca tgtttacaaa
    17941 atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa acggcgttca
    18001 atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta tttttccatt
    18061 tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc ataatggttt
    18121 ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt ccaaccaatc
    18181 taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt gtgcattgga
    18241 gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg cagttttgat
    18301 gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat cgtggctagg
    18361 gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc cgatatgtgg
    18421 tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa cagcagcttg
    18481 gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat taatcaccct
    18541 gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc attgtatggt
    18601 gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt ggtggtatga
    18661 tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca tttaatggcg
    18721 tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca gcagcacgtg
    18781 caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata cttgccggta
    18841 cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct aaatctttgt
    18901 ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg gattccagct
    18961 ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc ggatattctt
    19021 ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt acaccaatca
    19081 cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt tatgatgtca
    19141 aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac ttcttggaat
    19201 tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa gatagttttt
    19261 gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc tgatgggtcc
    19321 aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg gaaacgagtg
    19381 ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc gactaaaatg
    19441 actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg ctgaatacct
    19501 tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc accacctgca
    19561 ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa ggcaagaata
    19621 cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc aatcgttgtt
    19681 tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac ttgtacagct
    19741 ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga gttcgtgttg
    19801 atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa ttcagcacga
    19861 gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc gttacgcaaa
    19921 gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc gaaagtcgtg
    19981 ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg tttattcaag
    20041 gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat gtacataccg
    20101 atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat tgttttttct
    20161 ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc aggagtattt
    20221 gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag atgaacgact
    20281 tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt ttctacagcg
    20341 tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc tacacgttta
    20401 cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat attgttgaaa
    20461 gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc gatataacgg
    20521 taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg atattgacct
    20581 ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc tgtcatgaga
    20641 gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag gaaaacaatt
    20701 caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat aatagaataa
    20761 actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga aggaaagtga
    20821 aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg aatattttga
    20881 aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca gaagagataa
    20941 ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt ctcgcacttt
    21001 ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca gtattcataa
    21061 atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa agtataccag
    21121 aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac ttcctcgttt
    21181 cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg gttgtcacaa
    21241 ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg tgcaggaaga
    21301 tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc atggacagga
    21361 tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt gatagggaac
    21421 gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa aatacacgtt
    21481 tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt tagtacaccg
    21541 atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat gatcatactt
    21601 gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc catcaatccg
    21661 ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc ttcatctgat
    21721 ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc aaaattgttc
    21781 ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag caatgtttgt
    21841 ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc tctttgagct
    21901 ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg atcgctataa
    21961 gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag gatccccgag
    22021 atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt agggataaaa
    22081 ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa tgttgtacca
    22141 acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag attcttcaat
    22201 gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt tgctgaggct
    22261 ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt gctagtgtca
    22321 agctgtgtag ggaaacttga cactcccaca cctgtcatcg ctgacatcgt actagataaa
    22381 gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac tttgccattt
    22441 acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt acttaagtta
    22501 ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt gtgctgtgct
    22561 ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc cgctttgctt
    22621 gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat agtaatcgga
    22681 aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc agaagaaagg
    22741 gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt caagaaagta
    22801 cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca aaaactcatc
    22861 tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg atgggaatct
    22921 gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg ggccagttca
    22981 ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat caattccatg
    23041 atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa aaggatatct
    23101 gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc agataactga
    23161 tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc ttctgcccgt
    23221 tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac gttttcaata
    23281 atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga gttgtttcgg
    23341 taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat taccatatca
    23401 ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt accagaacca
    23461 ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac accatccaat
    23521 gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa ttgaagcata
    23581 atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag aacaatgatc
    23641 gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct agaaattttt
    23701 caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat ccgcaattac
    23761 agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc gcattgaatg
    23821 gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt acagatatca
    23881 tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc agatttgaca
    23941 cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc gggttcaaat
    24001 taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc ctacgtgtgg
    24061 aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc ggacaaacaa
    24121 aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt ctgcctcaaa
    24181 aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc actcgccaac
    24241 agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg atcgatgtac
    24301 atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc atcacagtac
    24361 ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt cacaattatg
    24421 gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt gttttctcgg
    24481 ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg gaaaaccaac
    24541 gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag gaatatcgat
    24601 ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata tgagtctggt
    24661 agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag aagtacagca
    24721 actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga cactgaggaa
    24781 tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa tccaaggata
    24841 tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat ttttatatcg
    24901 gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa aattgaacaa
    24961 actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg aacaaatcag
    25021 agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat tggatatcga
    25081 acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt ggctgaatat
    25141 catggataac gccataaagt acaatcaaat caatggacag attcatatca aactatttga
    25201 gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga atgaagagac
    25261 cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa tttctgggaa
    25321 tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac gtattgatta
    25381 tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac aataggaaaa
    25441 agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc gtttttttgt
    25501 gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc ttattgaact
    25561 acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga gagcgaaagt
    25621 catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg cttagttttc
    25681 ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac cgactttacg
    25741 agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag tattttcttt
    25801 taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat ttgcaatcat
    25861 attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa atccagcttc
    25921 gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga gcaaaaagtt
    25981 ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat gatttttagg
    26041 tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac ttggtggggc
    26101 ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat tgtaagttaa
    26161 ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt tcaaaaactc
    26221 ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga tatttaatga
    26281 aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt attgttctac
    26341 gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga tttttctatg
    26401 ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct gtttagaaat
    26461 atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg tttcggctaa
    26521 atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta ttccaataaa
    26581 gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac aatctttctt
    26641 gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta gaaaaagaga
    26701 ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg tttggcggac
    26761 taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag ctgacgatgg
    26821 tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg aaaaatatgc
    26881 cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt catttaggta
    26941 atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc ttccctagtg
    27001 aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc attgttctat
    27061 tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca ggagacagta
    27121 cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt gcgtgtaatt
    27181 tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat attttgttct
    27241 tattggcaaa atttatttat ccattgacta caccagacat gatcttagat tttaaagctt
    27301 gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa atgttcccag
    27361 tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg tggacaaatt
    27421 ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg actaaaaagg
    27481 atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac tatattatat
    27541 gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg acgtaatgca
    27601 catccagatt ccaactgttc ttcttgatgg tactgtgtta gcagcattaa gcaaggaaga
    27661 tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca gtgaatctac
    27721 actatatccg gttttgagaa ggttgaaaaa agaaggatat cttgagacct atgatgaacc
    27781 ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc gtcatttaga
    27841 agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat tggaggagag
    27901 cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt cctgaagacg
    27961 atcgttttga tttgatccag tattatgagg aatatttttt agattctgga aaaacattag
    28021 atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta aaaatcagtt
    28081 acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa agacaaattc
    28141 gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt ccactagcac
    28201 tggggtttat attggttatt gggggactat tggttgcttt actagcgctc atttttagta
    28261 tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc tcaggaatca
    28321 gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt ctgttcgcca
    28381 caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg cttttttatg
    28441 tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga gatctgcatt
    28501 cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg attaccggta
    28561 ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta ggcgctcaaa
    28621 agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag acaaaaaaaa
    28681 tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg gtcatacgcg
    28741 gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa ataaaggaga
    28801 aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc attagtttag
    28861 aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta tctgaattga
    28921 caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa aaagtgaata
    28981 ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca ataactgcga
    29041 aagatgaatt aggctcactt cgactagatg aggccaatgt ggataacttg atcgtcacag
    29101 gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga aaagtcatcc
    29161 taaacggtgg taatttatct ttgcatgaga tggaaaacag tggctatact atccaaacgt
    29221 ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg ttagaacaag
    29281 gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg acggatcaag
    29341 aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa aacagcaaag
    29401 atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt aaaataagaa
    29461 agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag aaggtcaaag
    29521 agaaaagtta aagacagaaa tggaaagatt acatacttat atcactcaac taagccaaac
    29581 cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt cagaaggtcg
    29641 ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga agaaagagct
    29701 ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg ggaagaagga
    29761 acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga tggatctaga
    29821 ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa aattggatta
    29881 tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg aagacaaaga
    29941 gttagaacta gaaggtgtaa tggcgcgcat tcgaaaaaaa attaaaaaag taacattaga
    30001 tatttattga aggataaagg agcgcaggaa gtagcatgag agaaaaaaga gcattttata
    30061 tcaatggtta tgcagggatt gtcggattgg tcgtattagc tttagttgga ttgtttttct
    30121 tctatttagg catgtggcaa gcgaaagttt gggcactttt tttaagtatt tttctttggt
    30181 tgatcacgtt attgttgtta agttcggcaa ctgtagtcag tcctaatcaa gcaaaagtaa
    30241 ttttgttttt tggacaatat ttaggtacga ttcgagaaaa tggctttttc cttacgatac
    30301 ctttggcaca aaaaatgact gtttcgttga aagttcggaa tttcaatagt tctgttttaa
    30361 aagtcaatga tttggatgga aaccccatcg aaatctcagc agtagtagtc tttaaagtcg
    30421 ttgatacagc aaaggcatta ttcgatgtag cgtattatca agattttgta gaaatccaaa
    30481 gtgagacagc cattcggcat atcgctagcc aatacccgta tgatacattc aatgagaatg
    30541 atttgacgct tcgaggaaac acgacggcag tgtctgacga gttgcaaaaa gagctgcaag
    30601 agcgtttagc agtagcaggt gtcgaagtaa tcgaaacacg cttgaaccat ttagcctatg
    30661 caacagaaat cgctagtgcc atgctccaac gtcaacaagc aaaagccatt ttgtctgctc
    30721 gacaaaccat tgttgaagga gctgtcacaa tcactcaaat ggccttagag caaatcgaag
    30781 atggacaaga aatcaatttc accgatgatc ggaaagtcca attgatcaat aatttacttg
    30841 tctcaatcat tacagacaaa ggaacgcaac ctgttatcaa tacaggcgac atgaaagacc
    30901 gtaagtgaca tactttttgt aagataggaa gaaggagttt aggacggatt attgtcttga
    30961 actctttttt tagttgtttt aaagaaaatg acaaaattga aagaagaatg atagttgatt
    31021 cgaaggcaga attgttcgct tttttctata atcagtatat aaagtaagag agaaggggaa
    31081 aaagtgaaaa aacttctgat cggtctactt gttttcatcg ttgtggtact tgcaggggtg
    31141 aaaatcgcag attacgtagt tatgggtgga gaagaatact atgtacaaat tacgacagat
    31201 ggtacaaaaa cagtttcaaa atcagactct ggacaagaaa cagtcaacta tagatatatc
    31261 ctgcctggct atacgaaaga gggtaaggaa aaagaactgg acttcaaggg acaattgact
    31321 cgaccgcttc gtaaaggagc atatttaaaa atcacctgga acaaaaataa aggtgtcact
    31381 tcgtatgaag aagtcgaaaa aaaagacgtt ccgaaaacag cactggaaaa actaattaaa
    31441 gggtagggaa agaaatgaac aaaatagtcg aagtacagaa tgtaacaaaa gtatacggaa
    31501 aaaacaatga gaaaaagacg caagcattaa gcggcatcag cttcgatgta gaaaaaggcg
    31561 aatttattgg gatcatgggc gcttctggtt ctggtaaatc gacacttttg aatattttat
    31621 ccacattaga taaaccaaca gatgggcata tccgaatcaa tcaaaaagat gtgaccacat
    31681 taaaaggaaa tcaactagca gattttcgtg cgaatgagat tggctttatt ttccaagact
    31741 tcaatttatt agaaagcttg acagcacaag aaaatatcgc tgttcctctt tctttacaag
    31801 gcgtcaaacc aaaagaaatc aagcaacgtg tccaaaaaat tgcagaacgt ttatccatct
    31861 ctcatatttt ggaaagctat ccttctgaga tttctggtgg acaaaaacaa cgggtcgccg
    31921 ccgcacgtgc attgatcaca caaccaacga ttctattagg ggatgagcct acaggtgcgc
    31981 tggattccaa aagtgcccgg gatcttttag atacgatgga cgaattgaat acaaaagatc
    32041 atgtatcgat tcttcttgtg acgcatgatc cgttttctgc aagttattgc caacgtattt
    32101 tattcatcaa ggatggcggt atccatcaag aagtgaaacg tggagatcaa agccgtgatt
    32161 ccttctatcg agaaatacta tctattcttg gaaacttgga acagtagggg gagattgact
    32221 atgctttgga aattatcact tactgggatc aagagccgtt taagagacta cctggtgtta
    32281 ttctcaggat tagttatggc atctgctatt ttttacatgt ttgaatcaat ggcaagcaac
    32341 gaagcttttt tgaaaagtaa tacgatcgtt gcggctacag ttatcatttt ccgattcggt
    32401 tctgttttac tagggatcat cacattcgtt tatattttat atgcaaattc ctttttgatg
    32461 acgatgcggc aaaaagacta tgcaatgttt atgatgctag gagcaaaagg cagaaaaatc
    32521 gcacagatga ttttttctga aacattctta gtagggatcg cagctacttt agtgggttca
    32581 gctgtcggtg taggattgac aagtatcgtc caccgattat tagtggatca gttagatctc
    32641 aatatttcgc attttacacc ttttagtatt cacgggttgt tgatcactgt tttgtttttt
    32701 gctgttttgt ttttattagc tgctgttgtc aatgcttttt cgattgtcaa aaaaccgata
    32761 ttagcattga ttcgtgcgga tcagacacca acaagaatca agcagcataa attcttgttt
    32821 ttccttgaag tagtactggg aattgctttt ctaggctgcg gttactatat gatgaaacac
    32881 gtggggacct atcaagtctt tggtatagga gtagccttag ttacgatcgt attaggaact
    32941 tatttcattt tccattctgt gatcattttc tttttatcat tactaaagaa atcagaacag
    33001 atttcattga aaaaattgaa caatttcaca ttgtcacagt taaactttcg tattcgtgaa
    33061 tatacacaaa tgctctcaat ggtcgctatg ctgtttgcct tggcactggg cgctttgacg
    33121 gtaggattag gttttaaaaa tgaaattgca gacttgacag aaaacaattc tggatatgat
    33181 ctagtgatga ataatcctcg ggcagaagat cagcaaaaaa tcaaagaact ttcaccaaca
    33241 ctttctgctt cttatactca aaaagaagat gcgggaacga tttactataa tgcaagtgaa
    33301 tttgcggctc aaccattgat catgataaaa catgaagatg gaacaccgcc gaagatcacg
    33361 aaagaaaaag ttcctgctga aaaaatgaat gaactgactg tacaagagga acttcgaagc
    33421 tatgaattgc cggaacagca agaaaaagaa atcaaggtag tcagtcaatc agaatttgat
    33481 caactgaact tgccacaatc cactttacag ctagttcagg taaaagactt ccaagcaaac
    33541 ctgaaaccaa tcgaaacact tgttgctcaa aataaaatga acaatccatc tttgcaaaaa
    33601 gtagagtaca gcaaccagaa atatgaaatg tatgaagtgt ataatggtct attctctggt
    33661 tttgaattta tgggattctt cctaggttta gctttcttga ccatgcttgc tagctgtttg
    33721 atgttcaaga tcttgtcagg aagcaagagt gacattgctc gttatgaaat gcttgaaaag
    33781 ataggaacac gacgcgggtt gcttaaacaa tcgatccgta gagaagtagg cgtgttattc
    33841 ttagcaccag gaatcttagg tgtagtccat gtcttattcg gattacaaat gtttggtcta
    33901 ttcatgcaaa atccatacaa agatatttgg gttccgttta cgatcttctt cgtattgtat
    33961 ttcatctact atgtactgac gacatggtta tatactggaa tcgtattgaa aaagagattg
    34021 tgaaaaagct gtcctgcatc aagtaataag aacagccaag caaaaatagc tcctcctatt
    34081 tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt tgttcggaaa
    34141 aagaggttgt gacaatattt ttgtcacaac ctttttttca cggtctttta ttagaatata
    34201 gtaaattttt tctcgttact cacggaataa gctgttaaga aaacggtttc catgtatact
    34261 ggaatcagtg caaatgaaag aaaagagggt gcaaggaaca tggaaatcaa tgaacaaaat
    34321 tttggcgaaa actatcactt gattacgatc acgaatcaaa atgggacatc tttgtctgtc
    34381 agtgatcttg gggcacgggt tgttcatttt caaacaataa tcgataatga aaaacgagag
    34441 ttgatcttag gttttgatga agcaaaagaa tatctagaaa aagaccctta tatcggtgct
    34501 tcaatcggac gtatggctgg acggattacg aatggcacat tcaaattaga tgatgaaacg
    34561 ttcgaagtaa aaacagaccc ggacactggt cattgtttac atggtggtgc acctagcttt
    34621 gaagccaagc agtggcatta tacagtcatc aatggtgaaa acgaagcatc ggtcattttc
    34681 tatcagacta gcccagacat ggaaaacgga tttcctggaa ctttagatgt agaagtacgg
    34741 tatacgctga cgaacgatga tacatggcga gtgacggtcc aagggatcag tgaccgcaaa
    34801 acagtattca atcctacaaa tcatgtctat ttcaatttaa ccggtgatcc agcacagtca
    34861 atcgacgaac atatactttg gttgaatagc cgattatttg ctacgctcaa taaagataca
    34921 acgcctacag gagaagtagt ttcagtaagt ggcacagcat ttgacttcca acgtcctaaa
    34981 aaattaggtg aggtctttgc cagtgatttt cgccaatcaa tgatagtggg cggtatcgat
    35041 catccatttt tgttgaaaaa acaatctggt ttactaaatg ctgcggaatt gacaagccca
    35101 gatgaaaaag tatcgatcca agtaaaaaca gatgcttcag gaatcgtgat ttttacagca
    35161 aattttggag aagatggacc ggaaatgcgc gggaataaat tagtaaatca tggcggtatc
    35221 acatttgaga ctcaagaact gccaggagcg gagcgacttg aaagttttgg agatgtcact
    35281 ctggaaccta accaagtacg cgagtttgta acggaataca aaataaatat aaagaaatag
    35341 aataaattaa aaagggagtg tgacagtgtc acatcccctt tttaatt
//
LOCUS       DACECY010000025        34781 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.026, whole genome shotgun sequence.
ACCESSION   DACECY010000025 DACECY010000000
VERSION     DACECY010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.026"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..2269)
                     /gene="esp"
                     /locus_tag="IWO38_001301"
     CDS             complement(<1..2269)
                     /gene="esp"
                     /locus_tag="IWO38_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ0313398.1"
                     /translation="MVSKNSKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
     gene            complement(2886..3191)
                     /locus_tag="IWO38_001302"
     CDS             complement(2886..3191)
                     /locus_tag="IWO38_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313399.1"
                     /translation="MLNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDA
                     KISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3336..4541)
                     /locus_tag="IWO38_001303"
     CDS             complement(3336..4541)
                     /locus_tag="IWO38_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ0313400.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKDFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTGNLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5235..5621
                     /locus_tag="IWO38_001304"
     CDS             5235..5621
                     /locus_tag="IWO38_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0313401.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5719..5910
                     /locus_tag="IWO38_001305"
     CDS             5719..5910
                     /locus_tag="IWO38_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0313402.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6424..6756
                     /locus_tag="IWO38_001306"
     CDS             6424..6756
                     /locus_tag="IWO38_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0313403.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            6666..6869
                     /locus_tag="IWO38_001307"
     CDS             6666..6869
                     /locus_tag="IWO38_001307"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0313404.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            7053..7208
                     /locus_tag="IWO38_001308"
     CDS             7053..7208
                     /locus_tag="IWO38_001308"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ0313405.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
     gene            7484..8824
                     /locus_tag="IWO38_001309"
     CDS             7484..8824
                     /locus_tag="IWO38_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0313406.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(9065..9472)
                     /locus_tag="IWO38_001310"
     CDS             complement(9065..9472)
                     /locus_tag="IWO38_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ0313407.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(9661..10488)
                     /locus_tag="IWO38_001311"
     CDS             complement(9661..10488)
                     /locus_tag="IWO38_001311"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HAQ0313408.1"
                     /translation="MPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPIPII
                     QAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEEQDI
                     RGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSKILN
                     TNLFNIDETSYSMGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLTDKN
                     FPGNITRAKGFLWFAQHSDFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKNPN"
     gene            10797..11015
                     /locus_tag="IWO38_001312"
     CDS             10797..11015
                     /locus_tag="IWO38_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0313409.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            11036..11671
                     /locus_tag="IWO38_001313"
     CDS             11036..11671
                     /locus_tag="IWO38_001313"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0313410.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(11864..12103)
                     /locus_tag="IWO38_001314"
     CDS             complement(11864..12103)
                     /locus_tag="IWO38_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313411.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(12127..13650)
                     /locus_tag="IWO38_001315"
     CDS             complement(12127..13650)
                     /locus_tag="IWO38_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ0313412.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            complement(13668..13790)
                     /locus_tag="IWO38_001316"
     CDS             complement(13668..13790)
                     /locus_tag="IWO38_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ0313413.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            complement(13820..14803)
                     /locus_tag="IWO38_001317"
     CDS             complement(13820..14803)
                     /locus_tag="IWO38_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0313414.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(14827..14976)
                     /gene="rpmG"
                     /locus_tag="IWO38_001318"
     CDS             complement(14827..14976)
                     /gene="rpmG"
                     /locus_tag="IWO38_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0313415.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(14997..15374)
                     /locus_tag="IWO38_001319"
     CDS             complement(14997..15374)
                     /locus_tag="IWO38_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313416.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(15406..15585)
                     /gene="rpmF"
                     /locus_tag="IWO38_001320"
     CDS             complement(15406..15585)
                     /gene="rpmF"
                     /locus_tag="IWO38_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ0313417.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(15600..15869)
                     /gene="rpsN"
                     /locus_tag="IWO38_001321"
     CDS             complement(15600..15869)
                     /gene="rpsN"
                     /locus_tag="IWO38_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ0313418.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(16392..18164)
                     /locus_tag="IWO38_001322"
     CDS             complement(16392..18164)
                     /locus_tag="IWO38_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0313419.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINM
                     VPAFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     MQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(18118..19872)
                     /locus_tag="IWO38_001323"
     CDS             complement(18118..19872)
                     /locus_tag="IWO38_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0313420.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(19982..20551)
                     /locus_tag="IWO38_001324"
     CDS             complement(19982..20551)
                     /locus_tag="IWO38_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ0313421.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(20569..21993)
                     /locus_tag="IWO38_001325"
     CDS             complement(20569..21993)
                     /locus_tag="IWO38_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0313422.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(21995..22663)
                     /locus_tag="IWO38_001326"
     CDS             complement(21995..22663)
                     /locus_tag="IWO38_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ0313423.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(22794..23378)
                     /locus_tag="IWO38_001327"
     CDS             complement(22794..23378)
                     /locus_tag="IWO38_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0313424.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(23709..23942)
                     /locus_tag="IWO38_001328"
     CDS             complement(23709..23942)
                     /locus_tag="IWO38_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ0313425.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(23957..24907)
                     /locus_tag="IWO38_001329"
     CDS             complement(23957..24907)
                     /locus_tag="IWO38_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ0313426.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(24904..25746)
                     /locus_tag="IWO38_001330"
     CDS             complement(24904..25746)
                     /locus_tag="IWO38_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0313427.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(25879..26604)
                     /locus_tag="IWO38_001331"
     CDS             complement(25879..26604)
                     /locus_tag="IWO38_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0313428.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIVHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            complement(27065..27379)
                     /locus_tag="IWO38_001332"
     CDS             complement(27065..27379)
                     /locus_tag="IWO38_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0313429.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(27395..28990)
                     /locus_tag="IWO38_001333"
     CDS             complement(27395..28990)
                     /locus_tag="IWO38_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HAQ0313430.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(29013..29903)
                     /gene="iolE"
                     /locus_tag="IWO38_001334"
     CDS             complement(29013..29903)
                     /gene="iolE"
                     /locus_tag="IWO38_001334"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HAQ0313431.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(29920..30945)
                     /gene="iolG"
                     /locus_tag="IWO38_001335"
     CDS             complement(29920..30945)
                     /gene="iolG"
                     /locus_tag="IWO38_001335"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HAQ0313432.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(30969..31976)
                     /locus_tag="IWO38_001336"
     CDS             complement(30969..31976)
                     /locus_tag="IWO38_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0313433.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(32089..33249)
                     /locus_tag="IWO38_001337"
     CDS             complement(32089..33249)
                     /locus_tag="IWO38_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAQ0313434.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(33325..34779)
                     /locus_tag="IWO38_001338"
     CDS             complement(33325..34779)
                     /locus_tag="IWO38_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HAQ0313435.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
BASE COUNT        10889 a         6933 c         5319 g        11640 t
ORIGIN      
        1 aaatatcttt acttacagtt actgctaaat cggtcgtgcc tacagaacca tcttggtaaa
       61 caattttaat aggaattgtt ccagtaatcg gctccgattc tcctacacct ttagctggag
      121 tgaatgtaac aataccagta tttgcatcaa cacttgcatt accgaagtcc ttattataga
      181 catctaaaat tgaaaaggca tctttagcta aaggaacatc tggtttatca aatgtaggtt
      241 ttccactttg aactgtcgtt tctggagttg ttgttatagg tacgtatgtt gcatcatttt
      301 ccatagcttg agaagctttt acagcaaact ctacatccac gtcgaaagtt cgatttccga
      361 tcttaacagt tacaatacct ttcgtactat cagtaacgtt agaagtatca ggctctgttt
      421 tccatgaata ctctgcatct tcaggtaagt ttttattatt ccgaattgat tctttagcat
      481 ctggtttaaa tccaactacc acggtttgtt tatcaaccgc ttttggtgat tccttaataa
      541 cggttgaacc ttcttctggt ttatcaaaac ctggagaaac gatttggatt tttaattcat
      601 ctttcgcgat taatttactt gaatctacac ccgtaaattc aaattctaca gtatctgaaa
      661 ttggagcccc atctttttca tttggagcga tagttttttc tgctaataca gtaccttctt
      721 tatcaacaag ttgggctttg tgacctgttc ccgctaactc gtggatgaat actttcccct
      781 taactgttgt gtcaacatca tatacttctt ctaattcagg tttcccgata tatggattcg
      841 tgtctccgct ctcttcttta tcgtcagggt ttccatcacc atcaatatct tgcaaataat
      901 aataacctgt tccataagtg ttctggatta attttttatt cgtatccgac aaccaagaat
      961 caaagatgaa atcttcacca aatggtttat tttcttcaac ttgtgctgtt gctttcatat
     1021 cttctaagat ttcatctaat ggcttagtca attgtaaaac atatcgaata acgactggac
     1081 gtggacttgc attagcaaaa tcaataagtt tggtaagatc attatcagta attgaaccta
     1141 caccataatt ttcatgatct ttgcgccgtt caattgctaa atcagccact tttttccctg
     1201 attcaaaaga aacatcatat gttactttgt cagcctgaac catgtgcatc tgtatacccg
     1261 ctacatatgg aactaatcgt tcatcaattt tgtaattagc ataccaaggc gtgccctttg
     1321 cggaagctga atatgagaaa ttataatttt tcgttaactt atgatctaag attatagctc
     1381 cattagcacc tgtggatcct gaaccatctt ttgtttgagt attaggttcc tcatattttg
     1441 cacttactga tccatgttta aactggtcag gattattttc agatacatct aagctattat
     1501 agaaatcatc tgaatctgtt aaaaagtagc ccgtactatc aatacctggt acaattttat
     1561 catcgtcttg atgacttacc aagtagattc gatacattaa acgatcggtc ttgagattgt
     1621 ctatgcctat ctctttaaaa atttcatcta agctcttttc aaacgtaatt accccattag
     1681 gagcagtttg tgtatcagtt gtttcagccc cagcaaataa tccatcactt gcacgaatat
     1741 aattgacctt ccaaatattt gtaggtctac ccagcgaatc attaattctt acaaaggtcc
     1801 gtctagaaga tccaattcct ttctttggat cgacctgaat tttttctaca tatttagcta
     1861 atcgttcatc taattgaaga cggatttccc agtttttagt tgatgctgca atattatgtt
     1921 ttccgtaaac ttcaaatcca attgtattgc cttttagtgt gtctccagtt agttcttgag
     1981 gactaaattg taaatccgaa aagttataat ttttcgtata accaggattc tcctgaaaag
     2041 tattccccga agctactttg tcacctactt gtacggagct tatcgctgtt tcaagtgggg
     2101 tgcttggaga aatttcatct tgtgcttgaa ctagttcagt tgttcccaag acaagaccaa
     2161 caccaaccaa aacagatgca accccaacac ttaatttttt gatactgtac ttcaatactc
     2221 gtttctttgt tttttctaga aatactctct tactattctt gctaaccatt attcaaatca
     2281 actcccttaa tagttatttt cttagtaaga ataaaagata aatctgatac gtattattca
     2341 cagaatagga atcttactaa acttatgtta actagtttac ttaaagaatt tcatgcacat
     2401 tacaacaaaa aaagtacatt tcttcatgtt tttttcggat atttgtgctt tttaaccatt
     2461 cagcagctga aaacatataa aaagatatgt atgtattctt tctgaaatta agttcatttc
     2521 tatgcgatga agcatgtttt caaaatatta ctctttctta gattaaactt cagagtattc
     2581 aaatagcgga ataaggagcc ttatcccgca ttactgaatg agactgtgac ataagtaaaa
     2641 aggtgacttc aaattccttc ttcggaattt gaaatcacct tctttataat tataaagtat
     2701 cggtttcaca gcattcctga atgattccta gaatcttaaa cgaatgattg gagttatcaa
     2761 cattttttca ctcgttttta gcgaaacaaa aaatgacatt tgtcacagcc tcattaaatt
     2821 attgattctt tttctttata attgaattgt ccatatttaa agtgtctagt actctacatg
     2881 aataattaat tgctttcact caaatactta taatctatta acttgtagtt gtttaacttt
     2941 tctgaaaaag ttagacccac gtaataatct tcccagcttt ctctttcaat taactgatcc
     3001 aatttttgat aatccataaa ttcatcatgt attaaaaaga ataaataact tgatatttta
     3061 gcatctagct taatcagatt aaataatgtt tgcttaaaat tgctaccatt aatatgactg
     3121 atagattctc tagcttcttc acataaactt tttactttat taattactac tttgtcatat
     3181 tcatttaaca cactattatt accattactt agacgctcag attctacgaa acaactcaaa
     3241 tcagaaattt cccaaaaaat tttttgttaa aatccaattt cctcaactcc tccaaactac
     3301 gtaaatgata tagtctcata ataaaataaa acctctcagc taatgttgtt gaaattatcc
     3361 actaaaccta attgatattg cttaggagat acacctgcgt actttttgaa tactctcgaa
     3421 aagtgtgaag aatctgaaaa tcctaaacct aaagaaactt cactgatagt atggtttgat
     3481 ttcaacatta ttttagccgt agaaattttt cgcttagaga tataattata aatcgtaata
     3541 tgcatttcat tcttaaagct ttctcttaat ttagtttcac ttacagaaaa ataattagct
     3601 atttccttta cagtaattct tttatagata tttaagccga tgaattgaac gacagaagaa
     3661 attaagggag aaagatgctc tatcttagtt tttccgattt ccttagtata ataaacaatc
     3721 ccagaatccc tcacttttaa gacctcaggt aacgatacag ctaattcgtt ttcttgtatc
     3781 aaagcatctc tactttgata cgcttctatt atatccattc ctaatgttat agacgtttgt
     3841 gctaactttt caaaaacaat gatagaataa tttttttcag accgaatcgt atctccacta
     3901 acacttggaa ttattccatt actcagatta aaaaccatgt tttctaaatt tcgaatatct
     3961 ccatttttaa catattccag tattttattt tcataaagaa acagatatat ttctggatcg
     4021 taattttgtg ataacagatt atctattttg atttgctcga tagttttact tagatttcct
     4081 gtatattcaa ttaacggttc atggaataga tcttctattt ttccagtaaa aaaataatga
     4141 actaaaatca gaatatctcg aatgtctcct aaagaataca aagtcaaatt ttgcaatgaa
     4201 ttatagagtc gttctctatc agccattgca acattcaaat tgttcatttt tagtttaaaa
     4261 aaatctttat caatattatt tgctctaaat ggaccaaata agtaatatct ggttttaaac
     4321 atgtatagaa gaaacatctc attaaaatca ccagtaaaga aatggaaatc tttagttttt
     4381 ttaatattat cagttagtaa ctttttataa tcataaaaaa aataagtagt tctatctgag
     4441 cgatactctt ttataatact gaattcggaa ttaaaaacca cgataggtaa ctttaaacaa
     4501 acatgaagag actttaataa gacttcttta tctgaaaaca cttttgtttc tctctttcct
     4561 ttctacatca ttaaatagta ttttatacgt tatctaaaat actattcctc tgtcaaaata
     4621 agctaactta cttcattata aaatatccac ttatcgcttt catgtatatt ccaccgtaaa
     4681 aagtacattt tatcaccgag aactcatcat ttttcatcat gatgaatact atttacatat
     4741 atctagttca aagacaataa ttattatgat ccgttattat ctaagcacta tacagataat
     4801 aggaattttt cgtttctaaa tactccaatt tgggaatcca ataaaaattt tcgttcaaat
     4861 acgtgtctat tcgaactctt ttctagttgt taattatgaa gggggaagaa ataaaaaata
     4921 caatctatac acgaataaga atattatcca aaaggaggtt ttagaaaaga tttattatac
     4981 gtaaaaagct caagacattt tcttagccat aaggacatgt ttgatattta agaagatata
     5041 tgaattttct tttctaagta taaagataac tatccattat caactaaaaa aacatttttg
     5101 aaaataaaca tcagcagtcc tatcaatgca tgtgagctta tagaagttat tcgcagcgca
     5161 gcataattgc attagcttct tttcgtgttt tttgcctctt ttccctttaa atcttaatgg
     5221 aggaggtgtg caatatgtca gataacgacg aacgcgtaaa tgcccagttg attcgattct
     5281 ttgaaaaggt cattttgaac accgctaata cttattataa aaaacaagaa gaaattcaaa
     5341 aacatgaaca acttgatcaa ctcccaccat atttgtttac taaagatact ataaaattta
     5401 aacaaccgat taatgtttta aatgtgactc tccttgttga agatataaaa ttagcaaaaa
     5461 tccttccttt gttgaaagaa cctgaacaaa tttttcttat cgaaaaattt atctttaata
     5521 aaactgataa agaaattgga aaggttctgg gtattacaag acagggtgcg acaaatttaa
     5581 aacatcgact ttacaaaaaa attcaaaaaa tgctggttta atatctgcat aataactaat
     5641 tttgtatgct tctttgctcg tcctcctcct tcccaaaaaa ataaatagat tataagttca
     5701 tgggaagaag gtaaatttat gaatacttta aaagaagatt tcattttagc aaagaatgga
     5761 aatgaagaag cagttgtggc cattctcaaa agatttagcg ctttggtcca taagcaatcc
     5821 tggagaactg gaaaatatga tcaagattgt tatcaagaat gtatgcttgc aatatatcta
     5881 gctatctcga aattcgaaat taaagaataa ctcaactaag ggagtctgtg acataagtaa
     5941 aaagatagtt tcaaatttcg aacaataaat ttgaagccat cttctttata attataaagt
     6001 atcgatttta cagcgttcta taatgattca tagaatctaa aacgaatgat tttaattatt
     6061 aacgtttttt aattcgtttt tagcttaaaa ataacttttg tcacagtctc actcctaaaa
     6121 ttactttatt tacgtacgaa gtgggcagga ggtcgaatcc agaactgggc catggtactt
     6181 caacgaggac ttctcctcta gaattgaaaa gcatgcgatt taccttccgt aacgacggta
     6241 gatttattca actatgtgtc caatcaaaat ttgtctgatt tcaaaaatag tcaagagaat
     6301 tttggttgtt aaaaactctt atctatcaac ttccacagtt tagttgacaa accctgatac
     6361 taagttgggc tatgatacac ttttcttgac tatcagttac gttaccggaa ggaatgaact
     6421 ctattgaaaa aaacatatga tcgtaaattt aaactaaaga tttcacagga tatccttgag
     6481 aaaaaagtaa ctaccaagaa aattgctgaa gaatacaata tctctcgtcc aacaatttcc
     6541 cgatgggttt cagaatatcg tcgatacggg aaaaatgctt ttgctggaca aggaaaaaga
     6601 ttacctgata aagcagattt ctacattctt gaacaggaaa ataaacgact aaaagaggaa
     6661 gacgagtgtt ctacgaacac aaagatcgat acggatctcc agggatcact caaaaacttt
     6721 acgatgaagg aatcgaaatc aataaacgtg ttgtagctgt tcttatgcgt gaaatgaatt
     6781 tatgcacaag aggatttcat cgacgcaaat cttctcatgg tagagaaaaa acaatcaaag
     6841 aaatggtaaa aaaaatgttg aatcgataat ttgaacagaa ccaaattgat gctgtttggg
     6901 tgacggatat tacttacatt ccatgtaatg acggtcgcct ctatttgtcc acctatattg
     6961 acttagcaac tcgtattcta cgttactata tggtggattc acacatgaaa aaagagattg
     7021 tcgtccgtcc attacaaatt tattaaggag aattgcctac ggtaattcat tccgatagag
     7081 gtagccagca tcggtctttt atttatcaag agctgttgac cgaccaccac atcacacatt
     7141 caatgagcca aactgggact cctgttgaca atgcagtaat tgaagcgttt catcgatcaa
     7201 tcaaatgaga attaatcgac tccaacaaat ataaaagtaa aatagagatg aaggtgttaa
     7261 tccaagatta tttagaagac tactacccaa acaaaagaat ccatacaaaa tttatgatga
     7321 ctccttgaca atttgaagcg aagaaaataa gggatctata atatctagtg ttggtgacgc
     7381 cttctgttgc caatactttt tcttttttct aataaacgca aacagacacc ttgaaatcct
     7441 ttagaataaa gtcgacgaaa accattcaaa ggagtgtttc aagatgtcct attcagagtt
     7501 taccaaagag ttactagata ttctagacct aaatcttacc tttcatgaag atgcctttcg
     7561 caaagagcgc attaatgacg aaacatgttt tgtttttgat ggcacattga cctatcaacc
     7621 agaggaatgc ttccattgcc actatcaaaa taagcaaacg atcattaaat ggggctggaa
     7681 aaaagtatcc atcttattga atgatgtcag taactacaag accattgtgc ggattaacaa
     7741 acagcgcttc aaatgtaaac actgtggaaa gactttttta gcagaagatt cagttagcga
     7801 tcgtcgctgc tccattgccc gcagagtcaa acaagccatt cttgagctat taagtgagcc
     7861 tatttctatg tctttgattg cacgtatgaa gcacatttct ccgaccactg tcattcgaat
     7921 acttcgcagc cttcggccta aaaccgtttc tttgaaccag ccgttaccag aagtggtgtg
     7981 tttcgatgaa ttcaaatcgg tgaaaaacgt ctcgggagcc atgagctttg tcatgatgga
     8041 cggcaagacc catcaactga ttgatattgt ggaaaatcgg caactcaatc ctttacgcga
     8101 ctatttcttg cgctacccta gaaaagtccg tgaacaagta cgtctagtgg tttcagattt
     8161 ctatacaccc tatcgcacat tggtgaaaga gtgttttccg aatgcccgaa tcgtcgccga
     8221 tcgctttcat attagccaac acatcggtcg ggcctttacc aaccaccgaa ttcaagtaat
     8281 gaagaccttt aaaaaagggg atcgtcggtc gaagcactta aagaaatact ggagactact
     8341 tcaaaagaat gcgtgggaat tgaatggtca gcatcgctac tggcgtccgt catttcgaga
     8401 tcatctgaca gaagcggaga tagtcgatcg gctattatat tatgatgact ccttaaagcg
     8461 aggctacgaa gtctaccagg tttttctttc cgctatcaga agacaagatg ttcctgaatt
     8521 cgtcgcgctc ctaaaagaag actacaagga gttgccagag cactatcaac cagtgttcac
     8581 gaccttcaag aaatatcgaa cagaaatcaa acgagcgttg cgagttccct attccaacgg
     8641 ccctatagaa tgtttgaaca accatatcaa agtactaaaa cgaattgcct atggctttag
     8701 aaactttcaa aactaccgag aacgaatctt tttatatcga ggaaaatatt tcaagaaaac
     8761 taagaacact acccaactga ctaaagccag aacaacaaca cggctagaca agatagctgt
     8821 ctagccgtgt gagtatctcg attattctat tgaatttctt caccaacact cattgacgaa
     8881 gagccaaaat aagtaatcta accgactgtt aactaaacca ttgcaggtcc cgttaaagat
     8941 acttgtgcct gttgtcaccg cttctttttc ttgtccacaa gcgcttaaca cacttaataa
     9001 agcccctgct attattaata ataatatctt tttcttatta aaaacctcct catctatttc
     9061 cagattagta aatgtagtaa tctttgcttt cttttttctc aatactaata actaacttat
     9121 ggggtaaaca aatactcgtt tgaccgggtt ttgagatcca tccagttcta accgcgattt
     9181 gatcaggact attatcttct ttaactcgaa ttcttccatc tttaatctca acaatattat
     9241 attgtccctt cgcaggatga taattcacga gtttatgatt gatcttatcc aaatcgaagc
     9301 gatccatttc cttgccatca atttgaacga tggcatattt gatagcatct tcagttgttt
     9361 cattctcctt ttctatagag aatacaaata agggaataaa agagggaata atcaaaatgc
     9421 caataattat gacatcgcct atcttgacac cagcttttct taaaaatttc attaacgata
     9481 tctggctaaa catattagaa tttttccatg gaaatggtga aatttttcgg aaattatctg
     9541 cattaagaga atcagttaaa caaccctcaa gttgttcctt aatacattgt tgatccatct
     9601 tcataccaat aaaaactagt ttattaatgc gttcaccaaa caacggatgc caggcttcct
     9661 tcaattcggg tttttttgta taatcatttg acactcaatt ttaggtaaac tagctaccca
     9721 ataatttaat gattcaatac ttttatggct acccgatgtt tcaaagatcg tcgaaaaatc
     9781 actatgttgg gcaaaccaaa gaaacccttt tgctcttgta atattgccag gaaaattttt
     9841 atccgtaaga tgggcaaatt tttgtgcgtc aaggagtaac cgatttcgat agataaaact
     9901 tgagatacca tattcttctg tttcaggaat atgattttga tagccaagct ctaactcttt
     9961 gaaccagccc attgaatagg aagtctcatc tatattaaaa agatttgtat ttagaatttt
    10021 cgataagtct acttcacctt taattgtcct aattattttg gcattggact gtaacttagc
    10081 aattagaccg ttaattttgt aaatcgtctt ggttgacact aagtcactat tatttaaaag
    10141 taatatgtta caacactcga attgatcaat taacaagcca cgaatatcct gttcttcttc
    10201 aactacatta ccttcagctt ttctttcctg aatcttttca cccgtttcga aattatccca
    10261 aaaacgtttt acatccacta ctataataat ggcaccaatc tctaatcgtt gggtcaaatc
    10321 aatgtctaaa gggtttttag ctaatataat tgcttgaata attggaattg gtttgctgat
    10381 tcctgaagct tcaataacaa tttgattcaa gtcagaaaat tgcactaatt gatctagttc
    10441 aacaatcaaa tcttcgcgta atgtacaaca aatgcatcca ttcggcatcg aaacaagttt
    10501 ttctccagtt cttttaaaac ctttcttgtc gactaatacc ccatcaacat ttacttcccc
    10561 tatgtcattt acaatgacag caatttcctt tccgtgatca ccgtacagaa tatgatttag
    10621 aagtatcgtt tttcctgaac caagatagcc agttaagatt gtaataggta ttttttcata
    10681 gggaacctct ttctatacct tgaatttaca ctttaagtgc aacacataaa aatgagtcat
    10741 aatcgatatc caactagtat aagtttgtcg agaacactcc tagaaggaaa ataattatga
    10801 gccagtattg tcatcttact atactcaaac gagaaagaat ctttctgctt cacgaagaag
    10861 gattttctat tagtataatt ggcaacgaca ttcagcatag cccgtttact attttacatg
    10921 aactgagaag aaacaagaaa aaaactaatc accttccaaa gcggaaaaaa attatgctta
    10981 tcgaagaaaa aaatgcggac gttatcacat tctaaatgat ccgtgagttt ggtcaattgt
    11041 tcgtcattta tttgtcgatt ttcagtggtc gccagagaaa atctcgaatc gtttgaagca
    11101 tgaacatatg gacttttcaa ttagttttat cacgatctac cgtgcaattt gcaatgatct
    11161 tttcgataca gaacccctct cacacggcaa taaaggcctg atccgttcat tgcaccatcg
    11221 aggaaaaaca agacatacaa aaaatcatgt cgagtctcgt gggaagattt gccttactca
    11281 ttcgatccat ggccatccaa aatccgcaga tgatagaagt gattttggtc attggaaagc
    11341 cgatattgta gctggcaaaa ctagtggtac ttgtttagtg actttgacag acagaaaatc
    11401 atgcttcctt ttagcaggaa agaaggtccc cagaaagaaa gccgcttatg tgagcgagaa
    11461 aatgattgaa cttttaagca gtattccaaa tgaggcggtc aaaacgatca ctccagatag
    11521 aggaaaagaa ttctccaatc atttcgcagt tacagaagta ttgaataata tgccctttta
    11581 ttttccagat cctcatgccc ttggcaacaa tgaacaaatt aaaatacgaa tagactcatt
    11641 cgagaatact tgccaaaatc gaaagagata gctacggtag ataactcaac gataaatcag
    11701 atgattgaaa aactgaataa cagacctaga aagtgtttag attggagaac accttatgaa
    11761 gtcttctttg ataaagtgtt gcgcttgatt tgacaattca agaaataata aaaagcagat
    11821 caaatccaaa taagggaaaa atgatctact ctggtattat caattaattg gccaagctat
    11881 tatgccgttt tgctaaacgt ctacttcttc cagtattgac cttgataaaa caacaatcag
    11941 gatcccgttt taatactcta tgaactgtgt caatactaac atgtaaagtt gcagcaatat
    12001 cagctaatgt actcccattt aatctcattt gctttgcaaa ttgataacgc cgataagcat
    12061 caatattctt ggaatccata gacaataata cttttcttac catgtatttc ctccaaagat
    12121 tggattttaa tgggccatca tttcttgagc aatctcatgc tcagataatg aagctggata
    12181 gaaagtaggc caattatgca tttcattata aagtttttct tgactttctc caccaaagaa
    12241 aatatggaaa tgctctgcct tacttggtgc aattccatga tcgctaaatt gaatatattt
    12301 aaaacgacca gcacctgcat catcagtttc aaaattaaaa cgaacaccac ggtttccttt
    12361 ttcataattc aaaattttgt atccaacata tttgtatgtg tattgatgat gttcaccgtt
    12421 gactacaaaa tcaatggtgt tgtctttaat gttgatagta tcaatatctg tcttatagcc
    12481 agtagtataa taatctttgt actcggcagc tgtcatgtct ttattgagtt ttgctttgta
    12541 atcgaaaact tcatccaaaa ttccatcttt taacaacgga taaacagatt gccattcgcc
    12601 tgtataatca ctcaattcgc gatccttcac atcggcatct ttaaagtacc cattagcaac
    12661 tgtcttttca tccttcgctt ctttttctgg aactacctct tcaccagcaa ccatagttgt
    12721 tttctctaac gcttttagat tgtcttccat tactgaaaca taatcttcac cattatccat
    12781 ttgttctttt gtaagacttt caagaggatt caatacttcc aattttatcc cagcttcatt
    12841 ggccaaagtt tttgcgattt tatcattagc attcttttca aagtaaatat aattgatctt
    12901 atttttttta acatactctt ttaattcagc taatcgacca gaagatggct cttcttcagg
    12961 acttagtcca gcaataggaa cttgtatcaa tccataatcc aaagctaaat atccaaaggc
    13021 tgcatgttgt gtcacaaaac ttttttgttt cgcttcttta agactagtgg tatagtcagc
    13081 atccaaccgt ttcagtttag tcaaatactt ttcagcattt gtctcaaata cttttgcttt
    13141 tttcggatac aatttaacta actgatcctt gatatttgaa acttccttaa ttgccatttt
    13201 gggtgaaacc cacgtatgag gatctagttc atgatggtga tcttcttctc cgtgatcatg
    13261 atcttcttct tcactacctg gcaataaaat catgtctttt gttccttcta ctacattagg
    13321 cccagctttc ttccaacttt ctttagcttt tggtacccat gattccatgt tttcgttatg
    13381 atatacaaaa acatcagcat catgagcttt tgccatatcc ttagctgagg gctcatagtc
    13441 atgaggttct gatcccgctg gaaccattaa atccacgttc gcttcatcgc cgacgatgtt
    13501 tttggtgaaa tcatacattg gatagaatgt tgttacaatt tctaattttc catctttatc
    13561 attcgatact tttgcatcat tagtgctgga gcaagctccc aataatccta gagctgctat
    13621 cccaataagt cctacactga ttttcttcat tctttttcct tccttaatta tgctaatttc
    13681 ttcattttat tttgtttatg ttctttgata atttttaatg ccctttttct agttttgata
    13741 ttcgggcttt taagtcgtcg atatgcacta gatatatccg ttttctccat tagaaattac
    13801 ccctaatcaa tacttatcat tatttgcttt ccgccagtaa tttatcaaaa ctctgtttga
    13861 tcctttcttc atccaacatt tttccaatta gaataatagt ggtttggtct ttttgtcgag
    13921 tggtgtattg aaaattaacc tgttcgttta ccccttgtat ggcaactata aaatcttgat
    13981 cttgcaaagc aatcaatcct ttaaaacgat acaggctccc ttggttggtt aaaatcagcc
    14041 ggtcaattaa ttggaataaa aaatcttttt ttatgtcttg atctgaagtt aagagaattg
    14101 atttaaactc atgttcttga tgatgttgat gatagtgttt gccattatag tgattttctg
    14161 atgaatcact ttcaaaaact aagggatgat tgaatttttc caaattataa aaatcctttt
    14221 tttgaatcgt ttcactatgt gtgaacattc gaatatcgga taaagggtta atacttttga
    14281 tttctctctg taattttttt agtgcagata aattcaagtt ttgttttcta gacacaaaaa
    14341 tgcgatctgc tatgacaagt tgcttaaggg cttgtggttc tctttcaata agttcaaaac
    14401 tgtcactatc aataacagtc aataaactat cgatatatac tcttcctctt agttgaggcg
    14461 ctgttaaaag tgtttgaatg attggttggg gatctgctat acctgtagtt tcaatgatga
    14521 tttgtgaaat tggttgttga tgttcttcta ttgtttccag taaagaaatc aatattgcta
    14581 ataaatcact gcgaagactg caacacatac atccattatt tagttgtaac acccgttcct
    14641 cgctatgaac taataatttg tgatcaatcc caacttcacc aaactcattt tcaattataa
    14701 taatttcttc cctggggtag ggtgagactt gcaaggcttg attgattagc gttgtttttc
    14761 cagcccctaa aaacccagaa acgattgtaa ctggtatgcc cattttgtca cctttttacc
    14821 acttagctat ttcacttctt tgaaaattgc ccgtctgcgc aattttggcg aatacttttt
    14881 taattctaac cgttcagggt tattccgttt atttttgctg gtgagataca gacgttctcc
    14941 agtttcgaca cattccaaaa taatattttg acgcataaat cttcctcctt caatacttat
    15001 cgatcttcaa gatatgcttg aatttgttgt gcatcttcaa aagttaactc gccagattcc
    15061 aattgtgaaa ctaaattttc tttcatatca acaggtatct cccaataacc atcaatacat
    15121 tgaaataact cctcgtcata ctcttcaaaa attggataga ttcttaaaaa atcatcaaat
    15181 gataagcctt ggtcctcata gtaatctttg actaatccat tttcaattgc ctgttgggtc
    15241 ggtaaacccg tttcagtaat aattcctctc tctaccatcg aacgataaaa agtgcattta
    15301 tctagcgatt gctgggtcgt ttcaagataa tactttaatc cttcttcaac taaaggtggg
    15361 taaataattt ccattttgat tccctcctaa aagttatata ttaaactatt ttgaagctcc
    15421 tttaattact ttctttccat tatagtaccc ttttaaagat acatgatggc tacgtctata
    15481 atcaccattc gcttcgtcaa atgaaatttc tggttttact acagtttgat gagttcttct
    15541 taaccgtttc ttcgccttag atgtttttct tgctggtact gccatgatat ttcctccttt
    15601 taccaacttg ctttttttac tccaggaatc aacccttgat gggcaagttc tctaaactta
    15661 atacgggaca taccgaactt tcgcatatac cctcttggac gaccatccgt ctgatcacga
    15721 agcctcagac gattcggatt tgaatctctt ggcaatttag cgagagcggt gcgatctcct
    15781 gcggctttta attcctgacg tttatctgcg tacttttcaa tcatcgccat ttgtttcttt
    15841 gctttagcaa ttttcgactt ttttgccata tacctttata ctccttttat tttgatttag
    15901 acatctcaaa tcgtaatcgt tacgttttga cactgatact ttaccaacaa gagttgctct
    15961 tgtcaacaat aaataacatg aaataaaact catatgaata gataactatt tgaaaacgaa
    16021 tttatcggta catagagata atcaaatcat ttaaaagaat atgcagttta agctctgaaa
    16081 aataagaatt cacttttatt agtccaatca aacacaaata ccgttttaag tagcttttcc
    16141 tctacttaaa acggcaatat gaaagaaact ataacttttt attcaatctt ttatgtttaa
    16201 ctggtttatc gccaattttt taaaactttg attattcctt atattataag aatagacatt
    16261 aagtgaacta attcaatcat actgattctt tcgtgtattc aaaattagat gctgtttaat
    16321 aaaattattc tttttgaaaa agaattataa ctatatccca aaaattgtag gttaatttaa
    16381 agcctatttc atcatcgtga ttgagcttct ttttcataga cgaatttctc agatagttta
    16441 gttttcttta tgagattttt gtatatttct gaccttcgat acaactcact atgagtacct
    16501 tgatcaacta tttttccttg atccatcact acaattttat ttaccttttc aatagacttc
    16561 aagcgatgcg atataattag tactgtctta tcttgtatta aacgatttaa actttcctga
    16621 atacgttttt cattttccgc atctaaactg gcagtaattt catctaaaat taaaattggc
    16681 gcatttttca aaaaagcccg tgctatggat aggcgttgtc gctctcctcc agaaagtgaa
    16741 cttccgtttt ctccgaccaa tgtatgataa ccgtcaggta gtttttcaat aaagtcttcg
    16801 cagttagcta aacgagctgc ttttttcact tcctcatctg acgcttgact atttccaata
    16861 cgtatatttt ccaaaatact tgtattaaac aaagttacct cttgaaaaac aatagcgatt
    16921 ttgctaaaca aagaagctgg agatacacgt ttaatatcat acccatcaat taaaatacag
    16981 ccttcatcat aatcatacag tctagacact aatttcaaga tactagtttt cccggaacca
    17041 ctagcaccta ctaacgcagt cacttctcct tgtttagccg taaaagagat atgatctaaa
    17101 attggggtat tgttatcata tgagaaagag acatctcgca actcaatgtc aaaagatttc
    17161 agaggactat ctgatccctc ttggatactg gtttccttca tagctctaat tcgctgtatt
    17221 ttaggagcta aataaaatat ttcaagcact gcttctttca tagaatcgaa ggaatccttt
    17281 atttttattg cggctagtaa gtaaccaacg acgtagagta tagtcacttc acccgttaac
    17341 agtaagtgaa cacctactag aatgactact gctaaactaa catatgagaa aatagaggat
    17401 aaggccatga ctgaaaaagt acttaattct acctttaaat gaatcctttc actctcttcc
    17461 atttttttga aaagtctgtc ttgaactttt ttagatagat taaagctatt aatctcttgc
    17521 tgcaattcta tagtttcttg aaattcttcc gagttttctc tcaaagtatc gtaatattta
    17581 gtattggctt tcgtttggga tttctttgat aacaagatta agacaaaact aaataacgtt
    17641 ggcaaaataa cagctaatcc cattttgaca ttaccaacca aaagcatcac tgaaataaaa
    17701 gggaaaaaca gagccatacc gccggcttta ggtattgcat gactcatcgc atgctcaata
    17761 ccttcaacgt cagacatgat agtttgagat aaatctgata aattatgttt tgaaaaatag
    17821 gataatggta aatttgacaa atcgtctgca atttgcattc ttaaatgcgc actttcttta
    17881 taagtactat tatataagtt ttcatactcc ctatctaaca accaatacat gaaaagcaaa
    17941 gtaacgaacg atactgtaaa ataaagccaa ggcggcttta cattttctaa aactaattcg
    18001 tctatcagca tcattataat gaatgctggt accatattaa tgcagtatac aaaaaagcta
    18061 gaaatagatg ctttaaccaa atcttctgcg ccctgattag ttaaagcgaa tttgttttta
    18121 taaaaacttt tcattgtcaa gcctccaatc atttgcattc tcataattag tgaccaattg
    18181 tttatacact tgatgtttag ccattaattc attatgattt ccacgttcta ctattttccc
    18241 ttctgacaag acaagaattt catctacaga tttaatgctg gataatcgat gagctatcat
    18301 aataactgtt ttttctttca tcagatgttg aaaagccttt tgtagttcat actcattatc
    18361 tggatcaata gatgcactgg cttcatccat aataacaatc tttgcatctt ttaaaattgc
    18421 tctggcaata gctatacgtt gtttttctcc tcctgacaga taaactcctt tactaccaat
    18481 taaggtatgc tctctttcgg gaaatttttc taaaatcgat tcgcaaccgg ctaatcttag
    18541 cgcgtcaaat actttacttg ctggcgcttg aggatttgct atagaaacgt tatcgtaaat
    18601 actctgattg aatagtttcg tatcttgaaa gacaaaagca attgtgttaa taattgtgtc
    18661 ttttgaatag tgatttaaag gcttaccacc aattttaatc tgtcccttat ccacattgta
    18721 gaaacctgaa attagtttag ctatcgtact ctttccgctt cctgattttc ctactaatgc
    18781 ataaattctt ttttgttcta aaactaagtt gaaattttct aaaacacact cttctttgta
    18841 tgaaaagctg acattttcaa atgcaatgtc acaattttta atctgtgtct cgttcccata
    18901 ttttagttgg tcttgcttca tttctaaata aagggagtct agtgtttcga ctgcatatct
    18961 tgcttcaaac aagtgcatac ttacatacat tattctcatg aaagaaacaa aaataattcc
    19021 acttaaaaaa aacaacatca acaagtcaac agctaatatc ttgggagtgg taagagaagt
    19081 tataaaaaag gttataggaa ctatcaaaat gcaaactatg ccaaaaaata gccattgaaa
    19141 aagaacaaaa ggtgttttcc cactcaaaga atacttatat gcaaacttag cataatcgtt
    19201 aattgcttta ttcaaggctt taaatgatgt tactttagca ccaaatatct ttataacttg
    19261 aattcctcgg atatattcta ccgtttcaga gcttaacaca tctaacgatt cttgatattt
    19321 ttgaataaaa tttgtattct ctgtcatttt ctttaacaag aatacaccaa ttactaacat
    19381 ggcgattaga accaatccaa ttttaaaatt aatgaaaaag cctaaggcaa ctgataaaac
    19441 aggtgttact atggcttgag cactatctgg aatcaagtga gcaattgcca tatgtgtttt
    19501 agccgcgttg tcatcaattg tctttcgaat atatcccgaa gaatttaaat caaaaaaacg
    19561 aaaacttgct tctgttaaac cgctaattcc cttttttctc aaattagtct ctaagcgaaa
    19621 cccaaggata tgagaaaata agccagaaaa aatataaaat aaagctccta aagttaacca
    19681 agcaacagtt tgaaccgcat aactcttagc taaatattca tctcctacaa taattagaga
    19741 cttaagaaat ttgtaaatac tgtaatatcc atagactgtt aatacgacag aaaaatcaga
    19801 aaataaagtg gctaaccatc cgtagtattt cattttaggg acataactaa ataatttttt
    19861 gtatactttc attttttcct cccccgcatt caatttcttc tcttattcgt ttacaactgt
    19921 taatgctttc cgtaatagcg taaattagag atgtacaaac agatagaagg taaactacgt
    19981 tctatttttt gtattttttt aagacttttc ttccaatcaa tacaccaatc atagataaac
    20041 ttgctacaac taacactgaa atgattcccc atatacctac cgcatagtgg atcattgcat
    20101 ccattgttgc agcagattgt cctttatcta tccaatcttg acgaaaacga tctgtaaaga
    20161 accatatagg aatccactgt ccggcagcac tgcctaattg cattagagcc caagacgcaa
    20221 cttgaggaat tgctcgatca tatccaaata cagatagaat aatatcagct aagatcgcca
    20281 tactaatcga actgataaac caaggtaagt acgccccgcc cattaacgta aaaaataaca
    20341 ataaaacaat tgtgtaaata gtaaaaattc cttttctagg gtagttatag ctaatataaa
    20401 caaatatgat tccagataat aatccaaaaa tccctgggga tatgctgtga ccaaacgccc
    20461 caccaatact actaaccatt attgaaaaaa agtaaattac tagggaaata actgtagcta
    20521 aaccaataaa aatataatct tttgttttca tatatattct cctcaattct atttcgttat
    20581 gtcattaaaa atctgttcta tcaaagttga atcttctact ctaagtttta tatctttttt
    20641 tattccacca ttttttaaat aaataattct attgcatgtt ttataaagaa attccggatc
    20701 atgagtggct ataactactg cacccttttc ttttaagtct ttaataaggt cagcaatcct
    20761 ttccatatta atataatcca acccacttgt aggttcatca aatagaaata aattagtttt
    20821 tgacaatgaa gctatagaaa ccaataatct ttgtttttct ccaccggata aatcaaacgg
    20881 taattgatct ttcattttat aaagttccat tttttttaaa attttttctt gctgatcttt
    20941 actaactatt tcattcccca gggctaactc gctcctcact gattctgtga aaaattggta
    21001 atccatttct tgcattacta aaaacggcga tgacaaattt tttttatttt cgattatctt
    21061 tcctttggta gggcgaataa tcttcatgag tattcttagt aaggttgttt tccccacacc
    21121 gtttttccct accaaaccaa taacatctcc aactttgaca tcaaaattta tgtcttttag
    21181 aatattttca taagtaattt cttcaacttt taatacatct gcacttacct tttcgatata
    21241 gggaacttca atcgaactat taaaaatgtc aaatttcctt aatgcgttat tgttttcagt
    21301 ttttaacaac tgaatagttg tagtttttac agttctattt ttaacaaata gaacttgatc
    21361 aattaattct ttcaaatagt aaaaacgatg ttcagctata acaatagtaa atcctttatt
    21421 ttttaaatca caaagtaagt cagcaagttt cttagtcgct ttgaagtcca aatttgaaga
    21481 cggttcatcc aatataatta atttagagtt tagcgataaa ctacatccaa tagaaatttt
    21541 ctgccgttca ccacttgata gcgaaaaaat gtttttatct aatatatttg aaagatctaa
    21601 taattcagct aattcattga ttcttttatc catatgctct tttgtaaatc caaagttttc
    21661 catcgaaaat actaactcac tcgttgtatt tgttgtaaag aattgtcctc taggatcttg
    21721 acttacgtag ccaatatttt ttgatatact ttcttgtgtt aaggattgta gcgagttatt
    21781 ttcaaataaa atttccccaa ccaattttcc gtcataaagt cttggaataa gtccattcat
    21841 taactttaat agtgtggatt tcccactccc agaagaacca gtaatcaatg taagactacc
    21901 tttttttatt gaaaaattaa ggtcaacaag cgcttgttcc ttaacatcct tatatgaaaa
    21961 agaaacattg ttaaattcaa acaattcttt cacctcaaat caaaatactt aagaaaaaaa
    22021 tagtaacgga aaacaggatg atatagtcta ataagctcat gtttgtttta tagtaagaac
    22081 ttcttttcac aggagattct atcccctttg ttattgcatt agcggtcatt tcttcagcaa
    22141 tcatagttgc acgtataaga acgggaacta acagaaattc caaatattgc agaggctgaa
    22201 aaaaagacaa gcgaatgttt ctcattttta aagattcttt aatcattctt atttcagaac
    22261 gataagtcgg aaagaatctg atcactactg taatacttaa cattattttt ttcggtaaat
    22321 gtattttttc taaagaggat aataattcat tgctagtata gacactaacc aacaaccatg
    22381 agatcattac gaccggcaac attcgaaata caatatataa ggatgtccca aaaaatatag
    22441 taaaaaactc tatcttgata gcatcaataa ttttcattac taaaaacaga actgaataaa
    22501 tcaagatgaa ataaatacat gtctttattt tacgaaacat caatactaaa actgtagcaa
    22561 taatgaaaca ccccacttga aatatatttc cctttcctaa aaaaataatg accggaaaaa
    22621 aaatatttaa agtaatcaag gttaacggat tgatttttga cactctttca cctccaaaaa
    22681 ataagatcgc actgttatct taagctgata aaaatgcacc gttattcaga taacagtgcg
    22741 atcttatcaa aaagcataac tgagaacaat tctcaatgca agcttttttt taattaactc
    22801 agtaattcaa tccacccagg aacagaaaat tgatacaatc ttctggttaa tagcatagtc
    22861 ttttcttttg aatagctatg cttcaatata tcatagaatg tagcgtagaa tgagtaaatg
    22921 tagaagtgga tttcttcttc attaaagact aaattttgat tgatcattcc gatagtcttt
    22981 aacttatcta gacaacagac tgtctttttt gtcacaaatg atgtaatttt ttcaacaata
    23041 tcttgatatt tggttccagc tgattggaat aaaaccaaat cgaatatacg tttattctca
    23101 aaaatataat taataaatgg atctaattca ttgaagttct ctctccaact tttctctata
    23161 tctatatttt ctaagtttaa gaggtatttg gtaaactctt cattatacat attaataagc
    23221 gcattaatat gcggctgaat aatttcttca aatagcgctt ccttagaagt aaaatgttta
    23281 taaaaagcac ccgtagttac gcctgcggtc aagcacagtt gccttatctt agtttgctga
    23341 aatcctactt tttcaaaatg gcttagtcca ctttctatta atttttggtg agttgtaata
    23401 taatctctgg tttctatggg tacttcctcc taataagatg aattaactat attatcttac
    23461 acaggacttg aagtttcaat cgttaacctc tattactgaa ctcattcctt agagctaaac
    23521 taatgagaaa gagcaaaata actgatacaa aaagcaatcc tacaagaatt aatagactca
    23581 tctaaatcat tcaccttttc taaaataatc cgagaaagat ttaaaaaaat tatatccagc
    23641 tattaggata agtcctatca taatcactcc aatagccatt ttcttatcct ccctaaaaat
    23701 aaagcctttt acttaaaacc ttttttgtaa tcgtcaataa attttttccc tttatcagtt
    23761 aattgaaatc ctatatctaa atagtcctca tatctaagtt tagtcagcat ctcacttaca
    23821 gatgctggac taacacttag aacttttgct aaatcattat tttttaattt cttttttctt
    23881 tttgataatt cctgaattgc tagtaaataa attagtatag tagttgtttt aatttgtacc
    23941 atacctaccc tccaaattat ttcgaaaggc cttcagcaat tttatccagg ttccatttca
    24001 tcatcgcata gtaactatca ccatcttgtc ctttttctgc aattgaatca gtaaaaatcg
    24061 ttgaatagat aggaatattg gtatcttttg atactgtttt cattggtcta tcgtccacac
    24121 tgctttctac gaataaggag ggaacttttg ttgtgcgtaa tttttcaact aagtgtttta
    24181 tttgatctgg tgttccttct tcttcagtgt tgatttccca aatgtaagca gaaggcacat
    24241 tatacgcttt cgagaaatat ttaaagcatc cttcacttgt aacaatcatt tttttatcat
    24301 tcggaattga agcaaattta gatttagctt ctttgtctag agaatccaat ttttcaatat
    24361 acttatctag attttcttta tagaattttt tattatcagg atctttttcc gctaactgtt
    24421 tttcaatatt tttagcgtaa ataataccgt tttctaaatt taaccaagca tggggatctt
    24481 ccttcccttt ctcactctga ccctctaagt aaataacatc tatgccatca cttgctgcaa
    24541 aatagtcttt gttttcctct ttgttcgcat tttttactaa ttttgtaaac caagcatttc
    24601 ctccagtttc caagttaaca ccgttataaa aaatcaaatc tgcttttgaa gttttttgaa
    24661 catcttcagg caaaggttca tattcgtggg gatctttccc aataggtacg atactgtgta
    24721 aatcaatttt atcttttgct atattttcag taatatctgc taaaatcgaa ttagtaacta
    24781 ctacctttaa tttttcgttc gaagccactg tatctttgtc tttactattt gttgaacatg
    24841 ccccgaatat aaaaagagca atcaaaatcc ccagacttcc cagtactgct ttccattttc
    24901 tcatcataac tttctctcct tctttcttaa aaatttttgt tttggtgaga ttagaaaact
    24961 aattacaaat aagaatgccg ctgttaaaac aatacttgat cctgctgcta tgttaaaact
    25021 ataaccaatg aacaaaccta gcaatgatga gcacgctccg actgtcgccg aaataaatat
    25081 catagttttt aaactgtttg catataaata tgctgtagca gctggtgtaa ttaacattgc
    25141 aacaattaat atagttccca cactttgcat agctgttact gctactaaag ttaatagaat
    25201 cattaataga taatgataaa aactgacatt catccccatt gcttttgcca ttaatggatc
    25261 aaaagaagtg attaataatt ctttaaaaaa tagaacaact gtaaccaaca ctaaaacaga
    25321 aacgccaatt gtaatccact tatcaatatc ttgtacagct aaaatatttc caaataagat
    25381 atgaaataaa tccgttgagc tatttgcaac tcctataagg ataactccta atgctaaaaa
    25441 agaactgaaa gtaataccaa ttgctgtatc tccttttatt acactattgc tttttataaa
    25501 agtaataatg atagaagcta atagaccaaa tacaatcgca ccaataaaaa aattaatacc
    25561 taaaatataa gaaattgcta ctcctggcaa aacagcatga gaaatagcat cacccataag
    25621 agacattcct cttaaaataa taaaacaacc tactgttcca gaaacaatcc ctataactac
    25681 agatgtaatt agagcattct gtaaaaagtg aaagtcatac agaccattaa taaaattttg
    25741 aagcatatta ttccccttgt atattagagt tagggtttta accttccggt tttgattgga
    25801 taaccctatg aaccatcgtt attttaattt ggtattaact catttctttt gagataatat
    25861 atttattgga ttgagaggct aagccgttcc cccctctcca ataaatatag tatctccata
    25921 ggcttttttt aaattctctt cattaaatac agattctaca cttccgtgaa caattagatt
    25981 tcgattcaaa agaataatat tatcaaaata cttctttacc ttgcttaaat catgatgcac
    26041 aattagcact gtttttccct gatgtttcaa ttcttttaac gtatccataa ttattcgttc
    26101 actaactaag tctatcccta caaatggttc atctaaaaat atgaaatccg cattttgagc
    26161 aagacatctt gctaaaagta cacgttgaaa ttgtcctcca gataattctc ctatttgatg
    26221 attagaatat tttcccatat cgaccttttc caaagccttt gaaactcttt gccattctgc
    26281 atttttaatt ctttgaaaaa ctttcattcc ggcatatgtt cccatagata cacattcttt
    26341 tacttttatt ggaaaagtaa aatcaatatc agctttttgc tctacatatg caatcttcac
    26401 cagtttttta ttgatatctt cattattaat ggagacctct ccctgatgag ggataatatt
    26461 taacattgct ttaagcaatg tagattttcc agcaccattt ggaccaataa tacctgttaa
    26521 agcaccttct tgtattttta aagaaatctc gttcaatgct aaaaaatcgt tataacaaac
    26581 attaagcttt ttcacttcta acataacttt ctccttctaa atgataatga ttatcacgat
    26641 catatggtat acaaaaatta tattttagtc aaccctaaat tttataattt agattttttt
    26701 ctgttgttga agattaggat gtcaaaatta tttgaaacta ctcgtatagt aaatagtaag
    26761 taattttcaa aacacgaaga aaaacaaaaa gaaaaaagga aacgccgaag catttccttt
    26821 cagtgattta atgagaacaa cacgatttga cttagagtct caaaaagtcg aataggtaca
    26881 ctcatttaag tgtctccatt cgactttttg gggatgagct ggctattgag gtccgcttac
    26941 cctattgaat aaatacaaga ttgggtattt ctaataagtc cgcccttcat attggggtga
    27001 tcggggcgaa catgccatct tttcatatac cacctcctcg acactcaata atcaaagatt
    27061 ctcgttaatc ttcttcgatg cttgcaatga tgttttcaat attcatataa cgaaaatctt
    27121 cttgtttgat ataaacgatc tgagcggtac atttttcctt tttgatgatt tcttttactt
    27181 tttcctcaga acgtgcgacc tgcggcgtta aaagtaaaac gtcatagtct ttataattca
    27241 gtttttcttc aacgtttcgg atcgttgcat aatctatctc atacgataga tttttggctc
    27301 tgatcgtttc aagcattttt gatacgagca gcgacgcact gattccggtc tcgcaaataa
    27361 tcaatagatc cttatgcata ataccctcct gtttttagcc tgcaaagcca attggtgaaa
    27421 atgccacata agttaaaata acaatgacta ccaatgctat agcagtcacc ttggtgtatt
    27481 tccaaggctc gatatcgacc tttgtatcga atgagttcaa cacaaattct ccatcaggtt
    27541 tcaatttgga aaccaccatc aaaatgataa tgtccagaac gaataatgcc gtcaaaacat
    27601 aaaggaaatg gatattttgg aaaagcattt ttgatagtgc atacaagacg atatgcacgg
    27661 caatcgtcgt ttttgcccca aaagcagttg ctttttttga ataaaaggca aagagaatga
    27721 tcacgagcag cggcatgttg tatagtccat tgaattcttg aacgaactga tacaatccag
    27781 ccggtgcaaa agaaatgaat ggcgctacga taatggagat aatccccaca acgaccgtta
    27841 ctgttttacc agcacgagcg atttgcttat catttgcttt cttattgata agcggtttat
    27901 aaaaatccag tgaaaacaag gtcgatgtcg cgttcaaagc tcccgcaaat gatgataaga
    27961 tcgcgccaaa gataacagcg gcgaaaatac caaagatcat tttaggcaat acttcactga
    28021 ctaaaaaagg atacgcgtta tctggatttg acattaaact atcgccaaaa atattgcgag
    28081 cgacgatccc tggaaagaca aggaataacg cacctaagat tttgaagacg ccgactagga
    28141 aagcgccctt ttgtgcctca gctaaattct tgccggacaa tgctttttgg acgatcattt
    28201 gattggtaca ccagaaatac agattgttaa acagcatccc tgtaaatagt gtcggccatg
    28261 gaacaacagc cgagtcgatc gatcccacag agttaagcag ccatggcgtg tttaccacaa
    28321 cgtgttcgat cccgccaaca aagctgccgt caccaagctt cattaagcct aaaattggga
    28381 ccagcagacc acaagccagc agaccgactc cataaatgga gtcactattg gcacttaacg
    28441 acaagccgcc gatcagcaga tagaggatgc cgacaatacc aattgccatc gctacaatta
    28501 tgatcgcgac gatcggctgg acgcccagca gctcatcaat atggaagatc ttattgaaca
    28561 ccagcgatcc agaataaaga accacgggta agaaagaagt catataggtc acaataaata
    28621 agatcgaaat aattcgtttg gtaaccgtat cataacgaat ctcgatgaaa tctgagattg
    28681 tattgatgcc gtatttaaaa tattttggca agaatatcac agccaaagct acgatcgcaa
    28741 tcgtagcagt tacttcccag gccattactt ccatccctgc atgataactt tgaccatttt
    28801 gtccgactat ttgttcggtg gacaggttcg tcatgatgat cgtgccggcg attggcaaag
    28861 cagttaagct ccgccctccc atgaagaacc cttctgatcc agaagtatcg acacttctgg
    28921 atctagaata ggcaaataac catacacaga tgacaatcac taggaaactg attatagaaa
    28981 aaagattcat cgtctagctc cttttcaata gattaaagac cggtgacctc tcgaatgtag
    29041 cttctggctt tgactgcata ttcaaatgga ttggctttag ccgggtcctg ttctgcttca
    29101 actacgatcc agccttcata gtcgctggca tggattgctg tccagattgg agtgaaatca
    29161 atcatcccat caccaggaac cgtgaagacg cctttcttca cgccgtttaa gaagctgtct
    29221 ttttcctttt ctacttcttc agccgtttct tgacgaatat ctttgaaatg gatgtgtttg
    29281 atacgatctt gatgacggtt gtaaaccgcg attggatttt caccagaaaa gaccaaatgt
    29341 cccgtatcga acaataattt aactttgtct ggatcagtca tttccattaa acgatcgatt
    29401 tcttccgtcg tttgaacgcc ggtacccatg tgatgatggt aaacgatctc catatccttt
    29461 tcgtgggcca aatcccctaa tttttctaat ccttccgcca gttttttcca ctcttcatca
    29521 gtgaaaactg gtttatcatc aaagactggc gctgtcataa tcccttggat actatgaccc
    29581 tgctcagcta ctacgatgac tttagcgccc atcgcatgta agaaatcccg atgtgctttg
    29641 aaggcttcgg cagtttcttc aaaaggttta gttgtcagaa acgcactgaa ccatgcactg
    29701 gcaaccttta agccgcgaat attcaaatat tgttccaaca cctctggatc ttttggatat
    29761 ttgttgccaa tctctgttcc ggtgaaccca gcaagggcca tttcactgat acattgttca
    29821 aaggtatttt cactaccaag ctcgggcata tcatcattgg tccacgcgat tggtgcgatg
    29881 gctaattgaa tttgtttggt catattctca cactcctgct tattttttta gttgtactaa
    29941 actcttcgta tcgaatgatt cttgacaagc caacgcgatc ttagttgatt gcaatccatc
    30001 ttctgcatta accgctggtt gacgtttttc ttgaatacat gagacaaatt cttgggcttc
    30061 acgaatgaag gcttctctga atcgttctgg gaagttttgt gaacatggac ggatgatccc
    30121 ttgatcgttc atgatcgtca caagattttt ctcaggcgct tgcgctaccc gcagcattcc
    30181 tttggttccc atgatctctg tttcgacatg atagccatgg gctgcgtttc tgccggcaac
    30241 tagtaaagct attgttttat cgctcaggct catcattgcg gctccagttt caagctcacc
    30301 agcctgatcc aattctggat acgccgcatt tttcccaatc gcccacactt gatcgacttc
    30361 ttggccggta aaccagcgaa ccaaatcaat atcatggatc gccatatcta agaacaagcc
    30421 gccgctgtcg ttattcatgg caaatttgac aaagctttcc aagccttcgc tcggatcgat
    30481 cccataacaa cgaatcagtg tgatttcgcc gatttcgcca tcttctacca ttttcttcgc
    30541 atactgatac gactcatcat agcggcgcat gaagcctaac atgaagacct gttcaggatg
    30601 ctcttcgatg actttttggg tctttttaat atcttcaact tcaaggccta atggtttttc
    30661 agtaaagaca tgcttgccat tttccatcgc caagcggact tggtcacagt gaaagcctga
    30721 tggtgaaaca ataaagactg catcgatcac ggaacttttg accatttctt catagctgga
    30781 aaaagtatgc ttgacgccta atttcttctg tgcgaatgct aattctgttt caactaaact
    30841 gcacgcagcc gtaagctcac aacaactgac acctgttgct aaattttgtg catgtacttt
    30901 tcctaatctt cctaaaccga cgatgcctac tctaatgttg ttcatgaaat acctccgttt
    30961 tctgagaatt actcattgta tagtgctggt cgttcatcca tttgaatcat tacttttgtt
    31021 tttgtttcac gagactcatg acaagccatc atggtgatcg ctgctacata cccatcccaa
    31081 gcggtaggac cgacaaccgc atcatttttg atcgaatcga tccacagctt gaattcataa
    31141 tcataggcat ccaaaaagcg ggtctgccaa tctggtgaaa caggtgtgta atttttttct
    31201 tccgatttaa ttcgtgtata ggctgggtcc gtcattccta cttctgccgt ttcgaaaacc
    31261 acatcacatt taatatcgta gccaaagcgg caattcacaa agatttccaa atcaatcgtt
    31321 acaccggatt ttgtttgaaa gatcagcagc tgcggatcat gcaaatcggc atgggagttg
    31381 ctggtttttt tcggcaagat catttgaaca gattcataat cttcatttag caaccagcga
    31441 agcacatcaa tctcatgaat cgcggtgttg ctgatcgcca tcggtgttgt gtagttttca
    31501 tctgcgctag gatttctatg ggcacaatgc atcattaatg cttctccgta ttgcttcgat
    31561 tcgattgctt ctttcagttc aatgtagccg cggtcatagc ggcgcatgaa gccaacctga
    31621 accaatttct tcccataagc aacttccgca tcaacgatcc ggcggcagcc tgctgcatcc
    31681 atcgctaatg gcttttcgca gaaaacatat ttcccatgct tgattgcttc taacacatac
    31741 tgctcatggg tcgggtccca agaagtcaca acaacgacat ccacatcttc agaagcgatc
    31801 aatgcttctc cggtttcaaa gaatttagca tcgacacgtt tggcaacctc tttagcagct
    31861 tttacattga tatctgagac tgctgtcaca taagcccctt gtgttttctg attgatcctg
    31921 tcgatatgct cgatgccgat tgcacctgtt ccgataacgc ctacttttac tctcaattac
    31981 ttatccccct gttctgttgt ttaagcacac atagtatgcg cttacaaaat tattcctgtg
    32041 tagcttttat tcttaaaagg tagagccctc tctttgctcc gccttttact acccataagc
    32101 ttgtacgtat aattccttaa tttgactatg actaggcacc tttttattat tttgcggact
    32161 tccgctggtc aacgcatccg cggccatttt gtctaaggct gccataaacg cttgctgatc
    32221 aataccataa tctcgaatcg ttgggatcga cagctgctca cagagctctt ggattttttt
    32281 aatcagataa tccgcagcct cttccagcga taaatcttcg gctgccggat aaataaatct
    32341 ggcgatcgtc gctaactcag tctgaatagc atcctttgag aattcaaaaa agaccggcaa
    32401 taacatcgca tttgatatcc catgaggaat gtgaaaaagt gcccccaccg gtcgactcat
    32461 tccatgaatc aatgtgactg acgcattcga aaaggcaatc cccgcctccg ttgaagctaa
    32521 gctcatttgt tctctggctt cagtgtcgtc gctattttca tatgcctgag aaagcgtgtt
    32581 caaaattttc ttcatcgctt ccagtgaaaa tagttgcgtt acttcattgg ctttgactga
    32641 aacataagat tccaaagcgt gacatagcgc atcgactccc gttgccgcgg ttacttttgg
    32701 tggtacagtt ttcgtaaaat cagagtcaac gattgagatc tgtggcatga aaacgagatc
    32761 cttcatcatc attttgactg ctgtttttgt attggtaata actgccacat cggttacttc
    32821 cgaaccagtt ccagcagttg ttgacactgc aatcagcgga atgcccgctt gcagctgatc
    32881 aaaatttctt gcatgaatcg cttcgagact taattgatta gcagccaaaa cggctaccac
    32941 cttggctgta tccagacagc tgccgccacc aaaagcaatg atatgatcac attgatgctt
    33001 ctgaaaaagc ttcagcgcat cataaacatt ttcttgcgtc ggttcggctt gaatgtctgt
    33061 atactccgct aattgatcat ctgtttttcc caacagctcc ttgatctttt gaagatgccc
    33121 ggtctgttcc ataaccgtat cactaatgat taacggcttg ttgcctagtt gttctgattc
    33181 ttccataaga aattggattg ttccgccgcc aattcttaaa ttggcaggca ttttcagttg
    33241 aatattcaca gaaaaaacct ctctctggct tatagcctat aacagaatcc tcgaagctct
    33301 ttaacagagc ttcgaagcaa atctttaaat ctcactttgc gcataacgtg ctgtaacaac
    33361 tttccgatgg gtataaaact caacactgtc tttgccattg gcatgcaaat caccaaagaa
    33421 agagtgcttc cagcctgaaa aggcaaacca agccatcggt gcaggcaccc caagattgac
    33481 ccccagcatc ccagcatcaa cattttctct gaagtagcga atcgctgatg cgttgttggt
    33541 atacagacac gaaccattcg caaattcaga ttcattcgtc agctcaacgg ctgccatcaa
    33601 atcttttacc cgcacgattg atgcgaccgg ggcaaaaatc tcatcctgcc agatcttcat
    33661 atccatcgtg acattatcaa aaatcgttgg tccaacaaaa tagccttttt ctggaatgtt
    33721 ttcgcgacca tctagtacca gtttcgctcc ttcatcgacc cctttttgga tatagtcaaa
    33781 ggtccgctgc tgtgcatctt ttcgaatcac tggtcctaaa aagacccctt cgtccaagcc
    33841 attgccgatc ttgatcgctt ttgccgcagc cttaaatttt tcaacaaact gatccgcgat
    33901 ttcctcttcc aaaacaatga cggaggctgc catgcagcgt tcaccagcag aaccaaaagc
    33961 tccgccaata aggtccttga ctgcccgatc aatattggca tctttcagca caacaacatg
    34021 atttttcgct ccagtcaacg cttgtacccg ttttagattt tttgttcctt cacggtaaac
    34081 gtattcaccg acattttttg atcctacaaa gctgatgcct tttaccagtg gatcttgcag
    34141 caatgtgttg acaacatcgt gggcaccata gacaacatta aaaacaccgc taggaaagcc
    34201 tgctttttca accagctcaa caattttctc catcaacaat ggtgtttttt ctgaaggctt
    34261 cagcaccacc gtatttcctg ccgccagagc catcgggaag atccagaagg gcaccatcat
    34321 tggaaaatta aacggtgtga tccctccgat tactccgatt gggtaacgat aattcgtgat
    34381 ctcaatatca gtcgccgctg ttgataaaga atctcccatc attaaattgg tgattcccgc
    34441 tgcgtgctcc acattttcga ttcctcgttg aacttctccc agtgactcgg acaatgattt
    34501 cccattttcc agcgtgatca aacgcgccaa ttcactttta tgatctgtta aaagctgatg
    34561 cagcttgaac agataacgtc cgcgaacagg aacaggaact tttttccaag tttgaaacgc
    34621 agcatcggca gctttaactg cttcggctgt ctctgctttg ctagacaacg gtacattcgc
    34681 cagcgcttct tctgtagccg gattcattac ttcttcaaac tctgttgagc gactagcgac
    34741 ccaacgacca tcaatataat ttcttaaaaa tcttgtcatt t
//
LOCUS       DACECY010000026        34643 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.027, whole genome shotgun sequence.
ACCESSION   DACECY010000026 DACECY010000000
VERSION     DACECY010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.027"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            591..1280
                     /locus_tag="IWO38_001339"
     CDS             591..1280
                     /locus_tag="IWO38_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0313436.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IWO38_001340"
     CDS             1267..2475
                     /locus_tag="IWO38_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0313437.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="IWO38_001341"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="IWO38_001341"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ0313438.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IWO38_001342"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IWO38_001342"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ0313439.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="IWO38_001343"
     CDS             complement(6578..7273)
                     /locus_tag="IWO38_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ0313440.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IWO38_001344"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IWO38_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ0313441.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="IWO38_001345"
     CDS             complement(8472..8663)
                     /locus_tag="IWO38_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ0313442.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="IWO38_001346"
     CDS             complement(8684..9574)
                     /locus_tag="IWO38_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ0313443.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="IWO38_001347"
     CDS             complement(9561..10328)
                     /locus_tag="IWO38_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0313444.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IWO38_001348"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IWO38_001348"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ0313445.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IWO38_001349"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IWO38_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ0313446.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IWO38_001350"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IWO38_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ0313447.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14944..15282
                     /locus_tag="IWO38_001351"
     CDS             14944..15282
                     /locus_tag="IWO38_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313448.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="IWO38_001352"
     CDS             complement(15450..16208)
                     /locus_tag="IWO38_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ0313449.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="IWO38_001353"
     CDS             complement(16226..17044)
                     /locus_tag="IWO38_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ0313450.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IWO38_001354"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IWO38_001354"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ0313451.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IWO38_001355"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IWO38_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ0313452.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="IWO38_001356"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="IWO38_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ0313453.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="IWO38_001357"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="IWO38_001357"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ0313454.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="IWO38_001358"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="IWO38_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ0313455.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="IWO38_001359"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="IWO38_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ0313456.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="IWO38_001360"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="IWO38_001360"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ0313457.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="IWO38_001361"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="IWO38_001361"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ0313458.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="IWO38_001362"
     CDS             27086..27385
                     /locus_tag="IWO38_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ0313459.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="IWO38_001363"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="IWO38_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0313460.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="IWO38_001364"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="IWO38_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ0313461.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="IWO38_001365"
     CDS             28352..30325
                     /locus_tag="IWO38_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ0313462.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="IWO38_001366"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="IWO38_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ0313463.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="IWO38_001367"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="IWO38_001367"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ0313464.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="IWO38_001368"
     CDS             33204..34496
                     /locus_tag="IWO38_001368"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ0313465.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10557 a         6734 c         6846 g        10506 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgttttcc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaacaaa cgg
//
LOCUS       DACECY010000027        34015 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.028, whole genome shotgun sequence.
ACCESSION   DACECY010000027 DACECY010000000
VERSION     DACECY010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.028"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            5..403
                     /locus_tag="IWO38_001369"
     CDS             5..403
                     /locus_tag="IWO38_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ0313466.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="IWO38_001370"
     CDS             387..1082
                     /locus_tag="IWO38_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ0313467.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGAYYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1666..2553)
                     /locus_tag="IWO38_001371"
     CDS             complement(1666..2553)
                     /locus_tag="IWO38_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ0313468.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2738..3577
                     /locus_tag="IWO38_001372"
     CDS             2738..3577
                     /locus_tag="IWO38_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0313469.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3773..4666
                     /gene="sgrA"
                     /locus_tag="IWO38_001373"
     CDS             3773..4666
                     /gene="sgrA"
                     /locus_tag="IWO38_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ0313470.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTGKVNNELDLVPII
                     PTKEQKRNAGSNLKSVLKQPDFVSRGIDHQEKAKETDNRKSDLPETGSRTLNKLITWM
                     GVLLILIVGASYFRSLFHRVK"
     gene            4964..5320
                     /locus_tag="IWO38_001374"
     CDS             4964..5320
                     /locus_tag="IWO38_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313471.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5323..6480
                     /locus_tag="IWO38_001375"
     CDS             5323..6480
                     /locus_tag="IWO38_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313472.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6506..6946)
                     /locus_tag="IWO38_001376"
     CDS             complement(6506..6946)
                     /locus_tag="IWO38_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0313473.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7106..7612
                     /locus_tag="IWO38_001377"
     CDS             7106..7612
                     /locus_tag="IWO38_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0313474.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7780..9693
                     /locus_tag="IWO38_001378"
     CDS             7780..9693
                     /locus_tag="IWO38_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ0313475.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9698..10699
                     /locus_tag="IWO38_001379"
     CDS             9698..10699
                     /locus_tag="IWO38_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0313476.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10901..11107)
                     /locus_tag="IWO38_001380"
     CDS             complement(10901..11107)
                     /locus_tag="IWO38_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ0313477.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11753..12601)
                     /locus_tag="IWO38_001381"
     CDS             complement(11753..12601)
                     /locus_tag="IWO38_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0313478.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12765..13667
                     /gene="gnd"
                     /locus_tag="IWO38_001382"
     CDS             12765..13667
                     /gene="gnd"
                     /locus_tag="IWO38_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ0313479.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13669..14670
                     /locus_tag="IWO38_001383"
     CDS             13669..14670
                     /locus_tag="IWO38_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ0313480.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14667..14951
                     /locus_tag="IWO38_001384"
     CDS             14667..14951
                     /locus_tag="IWO38_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0313481.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            14984..15781
                     /locus_tag="IWO38_001385"
     CDS             14984..15781
                     /locus_tag="IWO38_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0313482.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HVAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15795..16628
                     /locus_tag="IWO38_001386"
     CDS             15795..16628
                     /locus_tag="IWO38_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0313483.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16646..17503
                     /locus_tag="IWO38_001387"
     CDS             16646..17503
                     /locus_tag="IWO38_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0313484.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17525..18034
                     /locus_tag="IWO38_001388"
     CDS             17525..18034
                     /locus_tag="IWO38_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0313485.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYSVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18071..18472
                     /locus_tag="IWO38_001389"
     CDS             18071..18472
                     /locus_tag="IWO38_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313486.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18584..18937
                     /locus_tag="IWO38_001390"
     CDS             18584..18937
                     /locus_tag="IWO38_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313487.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            18971..19414
                     /locus_tag="IWO38_001391"
     CDS             18971..19414
                     /locus_tag="IWO38_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0313488.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19431..20516
                     /gene="uxuA"
                     /locus_tag="IWO38_001392"
     CDS             19431..20516
                     /gene="uxuA"
                     /locus_tag="IWO38_001392"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0313489.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20509..21222
                     /locus_tag="IWO38_001393"
     CDS             20509..21222
                     /locus_tag="IWO38_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0313490.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            21974..22174
                     /locus_tag="IWO38_001394"
     CDS             21974..22174
                     /locus_tag="IWO38_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0313491.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            22742..23305
                     /locus_tag="IWO38_001395"
     CDS             22742..23305
                     /locus_tag="IWO38_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313492.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(23366..23731)
                     /locus_tag="IWO38_001396"
     CDS             complement(23366..23731)
                     /locus_tag="IWO38_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313493.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(23807..24052)
                     /locus_tag="IWO38_001397"
     CDS             complement(23807..24052)
                     /locus_tag="IWO38_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0313494.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(24122..24376)
                     /locus_tag="IWO38_001398"
     CDS             complement(24122..24376)
                     /locus_tag="IWO38_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313495.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(24540..24863)
                     /locus_tag="IWO38_001399"
     CDS             complement(24540..24863)
                     /locus_tag="IWO38_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313496.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            25050..25123
                     /locus_tag="IWO38_001400"
     tRNA            25050..25123
                     /locus_tag="IWO38_001400"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25084..25086,aa:Ile,seq:cat)
     gene            complement(25508..26005)
                     /locus_tag="IWO38_001401"
     CDS             complement(25508..26005)
                     /locus_tag="IWO38_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0313497.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(26045..26854)
                     /locus_tag="IWO38_001402"
     CDS             complement(26045..26854)
                     /locus_tag="IWO38_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0313498.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            27188..28045
                     /locus_tag="IWO38_001403"
     CDS             27188..28045
                     /locus_tag="IWO38_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ0313499.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            28038..28940
                     /locus_tag="IWO38_001404"
     CDS             28038..28940
                     /locus_tag="IWO38_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ0313500.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            28973..29323
                     /locus_tag="IWO38_001405"
     CDS             28973..29323
                     /locus_tag="IWO38_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0313501.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            29357..30294
                     /locus_tag="IWO38_001406"
                     /pseudo
     CDS             29357..30294
                     /locus_tag="IWO38_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
     gene            30372..31265
                     /locus_tag="IWO38_001407"
     CDS             30372..31265
                     /locus_tag="IWO38_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0313502.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            31303..31866
                     /gene="amaP"
                     /locus_tag="IWO38_001408"
     CDS             31303..31866
                     /gene="amaP"
                     /locus_tag="IWO38_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0313503.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            31880..32068
                     /locus_tag="IWO38_001409"
     CDS             31880..32068
                     /locus_tag="IWO38_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0313504.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            32081..32983
                     /locus_tag="IWO38_001410"
     CDS             32081..32983
                     /locus_tag="IWO38_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0313505.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            33015..33257
                     /locus_tag="IWO38_001411"
     CDS             33015..33257
                     /locus_tag="IWO38_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0313506.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(33513..33884)
                     /locus_tag="IWO38_001412"
     CDS             complement(33513..33884)
                     /locus_tag="IWO38_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313507.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT        11828 a         5402 c         6828 g         9957 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcatattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta cttatttctc atttaaacga
     1201 tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt tttgaataaa
     1261 tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc tctagtataa
     1321 cagaataaga agaacaagtc cagaagggat atttttagtc ttttttaaaa taacttttca
     1381 gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa aaaataggga
     1441 agcatgaaag aatctactta atggcgcagt gattcatgtg atagaaataa aaaacttttt
     1501 taaattatat cctcatgatt gtaacaaatc cagtgatttg ttttggtaca aataaaaaaa
     1561 ggccccgatg aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga atttccaaaa
     1621 atcacttctc aaattttgcc acatccccaa ttgggtcaaa tttgtttatt tgaatatatt
     1681 ttttacataa ggatctttaa tagttacatt gtcttttttc atcactttac ggattgtttt
     1741 atccatgaat tccgtatcaa gagtttttgc ttcggtagca attttttcta gctcagattt
     1801 gtatttgtct ttattagaac ctttatcctg tttttttacc atttttacta gataatagct
     1861 ttgctgataa gtcgttggat ctgttacagg aatgatatca gaaacttccc cgttttttag
     1921 tttaaatgct gcttttttta cttcagtagg aatctctgga ttggttgaat caaaattcat
     1981 ttttccatca gtttccttta atgttttatt ttttccataa gcttgaacaa gcttggagaa
     2041 attttcccct tcatttgctt ctttttttgc tttatctgct tcttctttag tcgaaaatag
     2101 aatgatctgt gtactaactt ctggatagaa ttctttccaa gcagtgtcta gatctttatc
     2161 tgttaatttt acattggctt tcaaaccgta ttggaaagcc agcatctgtt taaagagttt
     2221 cttatatgtc tcttctgttt ggccaactgc ttttaattgt tccttgtagt ttggaccgag
     2281 ttttttgatt tgttcattat attcttttgt tacttccttt gaagaaatct cttttccgta
     2341 tacatcatca aatgctttga agacaatcat tttttttaat agtgtttgat tatttgattt
     2401 aacttttggg ttatcataaa aatcctgaac tgtaagtgtt ccacctttaa acgttgcaat
     2461 cgtttcattg gacttgctac aacctgagaa tagtaaaatt gcacatgctg aaaggcacat
     2521 aattagtagt tgttttttca aaaaatttcc catcctttcc ttattaaaaa cagtctaaca
     2581 gaagaattag ggagacagtc tttcattttc gtaaggttgc tgtagatatt cttagttcgt
     2641 aaaaatctat tctctctatg atggagaaca tttacatagt tttcacgaga caagaaagaa
     2701 aagggttgtt aaacttaata taaaggaggg atgaaagatg atcaggatta ttcggttgat
     2761 tggatctgtt ttaatagtct tagttggttt tgttttagga attcttgtca ataatgcact
     2821 tgcggatatg tttattggaa atagtgaatg gattggagca attgccggta cggtcggaca
     2881 tttagctgtt tttttactag ctttatttct tgctagtaag gtcgaaggaa aaagaataga
     2941 agattatggt gtttctgttc gaggaaaaga ctgggggtat ttagcgggag gtctcgttgt
     3001 tggaattgga gtgtttctgc ttattactag tccattatat ttgacggggg cgtatcggtt
     3061 agattcagga aacgcaaaca ttgtgccgct gataacgagt tttattttat ttattgcagt
     3121 gggggcatca gaagagcttt tattccgtgg cttttttcag catcagatgc ttagatttgg
     3181 tccgttaata gcaatgattg gatctgctgc attatttgct cttttgcacg gattgaatcc
     3241 taacatgaca tttctggcag ttttcaatat ctttttagca ggttgctttt tcagcgcact
     3301 tatctacagt acaggaagtt tatttacggc tattggtgct catattactt ggaattggac
     3361 acaaggagct attttgggta taccagtcag cggaacgcaa gaacaaggtt ttttctccac
     3421 tctgatccaa tcgcaaaata caataattac tgggggaaac tttggggctg aagcatctat
     3481 atcaactaca ttattcttgt tagtcttgat tgttggtttg ttactctatg catataaaac
     3541 aaagcggatg aagaaataca ctgctgttta tcaataaaag atgaagaaag caaggctgat
     3601 atcggtcttg cttttttaag tctgtcattg attaaacgaa aaagtaaaca aattcaagcg
     3661 aatacacgac atcttttgtt agattttcac aactgactgt cgtgttctca tcattttcat
     3721 tttatataat tcctttatca aatagaaggt aattatatgg agagtgatag aaatgaagaa
     3781 aacagcgacc gtattatttg ttattcttgg gctgagtata attgcagcaa tgattggaat
     3841 cagtacttct gcaaatgaac gggcaaatga gaatctttca tttactgtga aaactgatcg
     3901 gattgtttat gacatgacac aacaagtcat tactattcca gtcaaaccaa acaaaagcgt
     3961 gaatgctagt gatgtacatg ctgttctaac atatggttgg gatggtaatg gaagttcaga
     4021 aaaagttatt ggggaagttt atttaaaaga cgtacaatgg accgctggta ttgagtatac
     4081 aataatgatt tctgcagaac tatcgattga tgagatcaag agtaaagata aagttgattt
     4141 gattgttttt tatgatggac agatgacgat cacggagaat ttgaaaccaa gcagttggac
     4201 agttgttgga ccgccaagca gtacggatag cacaagtagt gaaagcagta cagaaaacac
     4261 gagcggtgaa agcagtacag agagtacgag cagtgaaagt agcacagaaa acacgagcag
     4321 cgaaagcagt acggagagta cgagtagtga aagtagcaca ggaagcacga gcagcgaaag
     4381 cagcacagga aaagtaaata atgaacttga tttggtacct atcataccaa ctaaggaaca
     4441 aaagagaaat gcagggagca acctaaaaag tgtgctaaaa cagcctgatt ttgtttctcg
     4501 tggaattgat catcaagaaa aagcgaaaga aacagataat agaaaaagcg atttaccaga
     4561 aactggtagt agaaccttga acaagctaat tacatggatg ggtgtgttac tcattttgat
     4621 tgtaggagca agctattttc gttctctctt ccatcgagtg aaataaagaa taaaagcaac
     4681 agatctaaaa ttgaataaaa cgatagattt ttgagtgaat atttcattcg agagtgctgt
     4741 attgcctggc gaagttgtag ctttgccaga tctatttttg tttactaaca tatttgagaa
     4801 acaggaaata acaggaacac aaatggaaaa taagagcaag aataattgac aatgcacact
     4861 gcgcatggta gtatctctga aaaatcagta acgaatctat ttgtacgtta ttcatgtagc
     4921 tgtcggaata agtctaaata atagataaaa aggagtatgt actttgtttg aagaatttat
     4981 tgatatcaat gaaaggcaag tatatcaatt tttgaactat tgttatgaaa gagacgagaa
     5041 attatatgtg gtgaaggata tcgctttaga tctaaactac accctggcta agatgaattc
     5101 ggtgattcaa caagcagaat cgttttgcga aaggtatcca gaatataaat tatcttttct
     5161 ttcagaaaac aagatgataa aagtcgagtt ttcttctcag tttttactga gtaaagttta
     5221 ttctatactt ttagagggaa cgattggtta tattttactg gattcacttt acaaaggtac
     5281 ttatcaatcc ttagaaaatc ttagtcaaaa aattatataa gtttgcgtac gattcaaaga
     5341 aaattaagag aacttaatac tattttgaaa aactatcatt tatcttgcag cttgaaaaaa
     5401 ggtaatccga tagaaggaga agaataccgt atccgatact tttttcactt gatgtattgg
     5461 cagtcattcg acgaaaatca ttctgatata ttatcaagta taaaaaataa acgtcaacta
     5521 ttagaaaaat ttgcgaaaca tgcgccatat acgcgtagta tagatagaaa gaaatggatc
     5581 agcctactaa gaatttcgtt gcaacgaatg aaacaaggcc actcgattac gtatgtccca
     5641 gaagagatga acaaatttgt gcatccattg ataacaaaag aagagtttac gcattacata
     5701 ctagagcctt tttttgaagc aaacttctgg tcgatgaatc acatcactcc aacagagcaa
     5761 gcttaccttt attttatgtt tggtgtaatg aacacctatt tggaagaaga tttgttggat
     5821 actccagata aagtacaaga aagttggggc tggattgaat cgtctattgt agaaaatttt
     5881 gaaaaaaact tcaagatttg ttttacaact tcagaaaaaa attatctata tgtcaatttg
     5941 gcaatgatcc atttgtattc aagggttttt gggacaaaaa agaaggtgga tgcttttgga
     6001 aaatcagcag aagacagaga ttttcagcat atatttccag tgatgtatcc aatcatgcaa
     6061 cagtttttta gtggggtagc tcgcagaatt ccagagctta atacttatta tgaaaagaac
     6121 agacggttga tttttcaata ctgtatgtta gtcagggata tttttataaa acacgaacaa
     6181 cctgtcatat tctatattca atcaaagtat ggtaaaacac aggaattatg ggcaaagaaa
     6241 aggatattat ctatgactga acgcccaata aaatatagtg catccgctga tcagttacct
     6301 gatattgtca ttagcgatta tcctatcgac aaaaaggcat atgatcaaac gaatacacag
     6361 atattttact ggaatggaca acctacaaaa aatgattggg cgcgtctgaa agctgaaatc
     6421 ataaaaatca ggaatgagaa aaatagatcc atattggaaa aattggattt cgttaattaa
     6481 gaaaagcaga ttgtttatgc caaaattaag acattgcaaa aagattattt tctttaaaat
     6541 cgacaagatg accagaaagc aagagttcgt tgagctcttg ctttctttta gttttagaaa
     6601 ggaacatcct gattttatct tcgctaaatc cagtcaaaag ttttgcagat tggtcgtaga
     6661 taatcggttt tttcaataaa gaaggggatg tacgaatctc aatatatagt tcactcaaac
     6721 tgagttcatc tattctgtga acaagatttt tataactaat agaatgagta gagagcattt
     6781 cggtcacgcc gttgtttgtc aattttagta agtggataaa ttcatttttg gttagtggtt
     6841 gacgaattaa gttctgttcg ataaacggta tatcatggac gataagccaa cgttttgcgc
     6901 gtcttgaaga cgagcagcca gaacctgtat acaatttaat catcatacat tcctccaata
     6961 gctaagtatt tatgaaataa taacatgaag aaatagtcga aaagtgccat ttagaggtgt
     7021 aaaataagaa aatgttgtca cgtataatgg aactagctag ataagaaata ctattaaatc
     7081 aagaaaaaag aaagaaggca caagtatgtc aaacacagcc gacgcaatag tagtgatcga
     7141 tttacaaaaa ggggtatgtt ttgattcttc gccgatacac gaattagaaa cagtgacagc
     7201 attagtgaat aaaagaatca aaacatacga aacgattggg aaaccagtta ttttcgttca
     7261 acacgaagat gctcaattag tgccacaaac aaaaccatgg gagctattgt cggaattatc
     7321 tattcctgag cacagttatt ttgtgagaaa aacccatgcg aattcatttt ataagactaa
     7381 tttgagtgtt ttattaactg aattggactg tcattcgatc gaattatgtg gggcacaagc
     7441 agagtattgt atagatacga ctgtgaagtt tgctcatgga ttaggatacc agcttatgat
     7501 gtgtagagga gcaacgacaa cttatgataa tccttttatg aaagcaaagg aaacaatcgc
     7561 tttttacgag aaaatttggg ataaacgatt tctgacattt ttggagcaat aagttgtttc
     7621 ttcgtttaaa aaaagcagaa tggatactgg tttatccatt ctgctttatt acgtaaaatg
     7681 agaaaataat cctgttctat ttttatggga taaaccattt attttttgta taaaaaatgt
     7741 ttgcgtttac atataattct cctagaaaca taaaaaggag tgaatagctt gagaacaaaa
     7801 caagtattaa agggagcgtt ggtcatctta tttgctgtaa gtacaagcgt cccgcaatgg
     7861 acagtatatg gagagagcat ttcttccgaa tcggaatctc gggaggtaga aaccacagaa
     7921 actactgatg ctggaaatga aacgaaatcg agtacttcac aaacagaaac aaatactgaa
     7981 acaacggttg agacatctgt tactagcaca gatcaagaaa cgaccgctac cacagataac
     8041 caacaaacag aagaagcaac agctacaaaa atgtcaaacg acgaatcctc ttctgctaaa
     8101 gtaagtgaca aagagaatgg gacagaaaat gatgatatcg gtaaaggtga aaatttcaat
     8161 ggtaaagaat atttgaatta cttcggtaaa cagatgacag aaatacttaa tggaccaaca
     8221 gaagacatca agtcaaaatt aatagagtta ttagtcactg agcttgctgt taatgattat
     8281 ggtgtagata aagagattat caaacatctg attgctactt atctattgta cgatatcgct
     8341 tttgcaaaaa acagtgtgtt gatgtcgatg cttggaggta cagatgatgg gacaacgcct
     8401 acagaaaaca caggcttcta tccaaataca aaacaacttc atcaaactgt actaaacaaa
     8461 caagatggca aaatgatgga tgtatatgca tattatgtcg atcaaggttc agataaaaca
     8521 gtcatgatcc atggcggatt tcgagggaat tggaataacg ggatcgttac agaagaatac
     8581 aatgattttt ataaggccgg gtataattta ttatttgttg attccagagc gactggtaat
     8641 agcggaggcg attatgtgac ctatggacaa tatgagtcag atgacgtcct ttactggata
     8701 aatcaagaag ttcgagagcg gccatctcaa aagattttac tttatggtgg ttcgatggga
     8761 gcagctacga tgatgtctgt attagcgaaa gatattcctg tcaatgtcaa aggaattatt
     8821 gaaaattgcg gatttgcatc aattgatgaa caactacgtt ttacgtacag ccagacagta
     8881 gcccccgcac taccggatgc cataaaaaat cagttagaca tcattgggga tcaagagcat
     8941 gaagatttat ttatgggcct tttgaaacaa tactattttg atcaggagat gcatttggat
     9001 acaaaagctg ctctacctac gattggaatg tcagattcat tgcctaaatt gattatccat
     9061 ggaacagcag atgatgtagt acctgtgtct aacgcgcaaa aattatatga gttatcagga
     9121 gggtataaag atcttttatt agtagaagga gcagggcatg gaaaggcaca ggaagttgac
     9181 catgctgcct acacgaaaca tgtaacggac tttttgaaag tagtgttcca tgatcagata
     9241 aacgtaaagt atgtggatga aaacaataaa agtctactca aagaccagga cgaaatccag
     9301 ctttacggag cttatggaga aaattatatg acagagcaaa agacattcga aggatacgaa
     9361 ttagcaaatg ttgaaggacc gactgaaggg atttttaatg agacgatacc aactattatt
     9421 ttctattaca aaaaaatccc agtagctcca ccaaaaaagc aagatccgac agagaatgca
     9481 gacaataagg gaaagaatga acctcgagag gatcaatcaa gtgatgatac gcaaccgatt
     9541 cagatgacag tgaaagtaca aaaaaagaca gatgcaaaaa aaagaatgtt gccgaaaact
     9601 ggagaaaaga aacattcgct tctcatttat tcaggtggat ttttatctgt tagcacgatt
     9661 atcacatgga tttggcgaag aaagagaggg taaatgaatg aaatggttca agcgaatagt
     9721 gattgcagta ccagttatca tcgtagttat tttagtcact gtatttttga ttaatcaaat
     9781 cacaccaaag ccgattagct tgcttgttag aaaacaattt gacacaagtg aaaaagaaca
     9841 agtatatgcc cgaccagatg atttccagca aaaaatcgaa caaatcgata taaaaaaaga
     9901 aatatcctat ccttcaaagt acaaaaataa taatatggat atctacacac cgaaagtgga
     9961 aaagaaaaaa ttaccgattc ttttctggat gcatggaggc gcatatgtag gaggggataa
    10021 aaatgattgt cgtgattatt tggaatatct atgttcagac acgcagcaaa tcatcgtaaa
    10081 tatagattat gaacgatcac cggaagcaaa acatcctaca ccagttatcc aattgaacga
    10141 agccattcaa gcagctaaaa aagagataaa agatcaagca gattggtcga atatttcgat
    10201 tggtggaaat tcggcaggtg cacaaattag tggagaatat ttgttagcac ttcaaaatga
    10261 agaaattaaa aaagctgatc atcttgatcc gactttggag aacaagcaga ttacgaagtt
    10321 tatttctttg tctggtctgt tggatccttc tgcacttact caagtaagtg ataaaatctc
    10381 tagctttctt tatgaaaaat gtggatgggc ttactttgat aataaggact ttgaacaatc
    10441 agagaaggtc aaaagtttag ccttggtcag acatgcggat cgttggacgc aaaacacatt
    10501 cttaacggat ggaaatacaa atacgtttac gaaacaaatg gaagaagtaa cttctgtatt
    10561 tgagaaagcg ggggtgaaag tcacaaaagt agattatgcg gagaaaaatg ctgttttgaa
    10621 ccatgaatat cagtttgatt tcagcaataa acaagcgcaa gaaacatatc aaaagttagt
    10681 aatgtttttc aaggagtaaa gtcttatgat gcaaaagagg ttgtaatctg tcacttagct
    10741 ccaagtcaca aggagcaatt tcaataaaca agcttttcat ggtttatgaa tattgtaact
    10801 tgtgactgag gagttagaga tggcaagggg caccttttat tcgaaatttg ggaatatcac
    10861 acgattattg tcacgatctc ttttacgtaa attttataag tcagatttgt tgttttgatc
    10921 gtttaaaaaa agcaacaaat ccgaagcctg cggaggcaag aagggacaaa aagccgagtg
    10981 gtaagcaaaa gaatgctggt tccactaata ttccgttgtt attgatataa ggctgggaac
    11041 aaagatgaat agcaaataaa atcaatgcac tagcgaaaaa aagcaaagaa atatggatag
    11101 ctttcataag cagcacctcc ttttttataa ttgtatgata aatagattaa gaatctataa
    11161 atgtgacaag tttgttagga tgtgaataaa atgatcatct agcgaacttg aaacaaagat
    11221 ggctaatgat aagcaattaa tgattcagca atcagatagg tacaaagttt accatcatac
    11281 gtctagacca ataatagaaa atatgggaat ataatagaaa agatttagga tacccccaaa
    11341 agaatttccc caaaaattcg atgtatccta aactttttta ttttacataa caatcatatc
    11401 aactgtgaaa taaaattaca aatgataacc taaaatgagg ccaaaagcca gtgttatcaa
    11461 ggaaatactt aattcaccaa aatttttttt aattttttag aaaataagaa acaaaaataa
    11521 taaaaaaaag aaaagataaa ttttttacta ctgtatacta actttgttat cgcatctaac
    11581 aagtaactcg aataatacca atacaacctt ctccaaagat gcgatgacat acgttctaac
    11641 ccaccgacta ttgatgttct gtaccaccct tgcagaacca aaaaagccac taccaaatat
    11701 ttggtagtgg ctttttttgt tttatggatt tttcgctttt tctgttatgg tgctattctt
    11761 tgatttcaga aatagtatgt cgaactttag cgatagaacg acgtgattct ttttcgttgt
    11821 aaaacatcaa aatcacatat agagagtcca aaatgcttag ttgagaaata cgggaagcta
    11881 aagcttcaga tcggaattct gtttcttcag aaatagaaat aaaaacgaca tcgccaagtt
    11941 tagcaaggga agaattagct tggctagtaa taacgattgt ttttgctcca gtttttctta
    12001 cggtttcagc aatatggatt gtttcttttg attgccctgt gtgagaaatc aaaacagcac
    12061 agtctttttc cgtcaataaa gaagcttcca tcaattgaat atggtaatcc gtgctgtgag
    12121 aagttgcgat aggggaacgc aaaaatttat gataagcgtc agttgctaca atctctgaac
    12181 cgccaacacc gaaaaaatag acacgtttag attcattgat aatagtagcc gcttttttta
    12241 gatccttttg ttgtaacaac ttttttgtgt catttaaagt tgaaatacta gactcgaaca
    12301 ccttttgcgc catagtgagc tcagtatctg ttttctgaat attttcgtgg atagaaatag
    12361 cagatagatc attttcttgg atcaacatgg ccattttaaa atctttaaat ccattataac
    12421 caagtttttt tgtaaattga aaaaaagtag agtcagcaat gcccagttca cttgctaaat
    12481 tgctaatagg actatgactt actttgcttg gattttctaa aatataatcg gcgatagcct
    12541 gttcttttgg gctgaagtcg ttgtacattg ttttgatttg tagtcttatg gagacgctca
    12601 tgagaattcc tcattttcat aataagatta tcattttttc cgttctcatt aatgttaaag
    12661 atacaaggag agtaggaaaa aatcaaacaa aaaatatttt tgtaaaatct atttacaaaa
    12721 aaattttttt gtataatgag ggtataagaa atggaggcgc tatcatggaa attggtgtaa
    12781 ttggtttggg aaaaatggga ttgaatttag ctttaaactt aaaagagcac catcacaaag
    12841 tacaaggact cgacatttca agtacagctg ttagtcaggc aagagaaaat gacattcctg
    12901 cttatcaaga gatctctgaa tttctagcta cttttgagca gaaaaaaatc atctggttaa
    12961 tgcttcctgc tggagagata accgaaaaca tgttaagaaa actttttcca aaactcggac
    13021 caggagacat tattattgaa ggcggaaact cttattataa agattcaatc cgtcgagcag
    13081 aagagtttca gaaaaatgaa atcggttact tggattgtgg gacttctgga ggaatcgaag
    13141 gagcacgaca tcaagcctgc ttaatgattg gaggagataa agaaactttt ctatccgtgg
    13201 aacaattatt caaagatgta gcaattgaaa gaggatatct gtatgctggt gcatccggaa
    13261 gcggacactt cttgaaaatg atccataatg gaatcgaata tggaatgatg caagcaatcg
    13321 gtgaaggatt ccaacttgta caaaaaagtg agtatgcttt tgacttggaa caagtcgcac
    13381 gtgtttggaa tcatggttcg gtgattcgcg gctggctgat ggaaatcatg gaagatcagt
    13441 taaacgaaca tcccaattta agtaattatc ggggaatcgt ttctgcatca ggtgaagcaa
    13501 aatggacggt tgaaacagcg ctagaaatgg ctgttccagt tccagtaatt gcaactagtt
    13561 tgttcatgag gaatctttca caggaagaag atagtttttc agcaaaagta gtttcagcac
    13621 taagaaatgg gtttggcgga catgaaatag ttgaaaaggg tgagtaaaat gccagattat
    13681 catatagcaa tcgttaattc cagcagtttc ggaagagtct ttccacacca cttggaaaga
    13741 ttaaagtcag ttggagacgt tcatttcttt actgttgatc aagagattcc aggaaaagaa
    13801 ctagcggagt tacttaaagg attcaatatc attattgcaa gtgttacgcc attttttcga
    13861 cgagattttt ttgaaaacaa agatgaactg ctattgattt cccgacatgg tattggttac
    13921 aacaatattg atttaaaagc agcaaaagaa caccagacaa tcgtatctat catacctgct
    13981 ttagttgaac gaaatgctgt agcagaaaat aatataacta atttgttgtc catgcttcga
    14041 cgcacgacac aagcacaagc aagagtcaag gacgatcgat gggaagatcg ggcggagttc
    14101 gtcggtagga cgctgtttaa caaaacagtt ggtgtgattg gtgcaggaaa taccggaagc
    14161 ggagtaatcg aaattttgcg taacggtttt cgctgtgatg ttctagctta tgatccttac
    14221 aaatcagatt tatacatcga aagctttggt gcaaaaaaag tagaacttca agaattactg
    14281 gaacaatcag atatcatttg tttatgtgcg aatttgacag aagagaacta tcacatgatt
    14341 tctgataaag aagtcgatca gatgaaagaa aatgtttatc tatctaatag tgcaagaggc
    14401 gctctagttg atgaagaagc gatgattaat ggtctgaaaa gcggaaagat cgcaggatat
    14461 gccacagatg ttttagaaga agaaccggga cgtcaagatc atccgtattt agcgtttgaa
    14521 caagtagtga ttacgccaca tatatcggcc tacacaaggg aatgtttaga agcaatgggt
    14581 gaaaaatgtg tcagagatgt cgaacagatc agtcagcacc ttcttcctga acgttctgtc
    14641 cagacaaaga gcaactatat aaaaggatga tgaagaaaat ggtgaacggt ttaaaagtga
    14701 aaacaggccc tcaattttat ttatatgaag aaggtggtat ttcaaaagtt tctgatctct
    14761 tgaaatcata cggcgcgaaa agagtactgg tcacccacgg aacggtatca tgggaaaagg
    14821 cgctacctaa attagtattt ttgaatgacg aaacgattca atttttttat catcgttatt
    14881 ctggagaatg tagttatgct gaagccagac gaatagctac aatcatcaaa aaaatgaaat
    14941 cgatttcgta gttggcgttg gcggaggaaa actgaccgat ctagtgggtt atagtgccaa
    15001 tcttgcaaga atcgatttcg gagtcatccc tactttagct agtaattgtg ctccatggac
    15061 acctcttgct gtcatgtata aggagaatgg acaagcagaa gggaaaacag aacactttta
    15121 cagacaagct gcttttttta taacagatcc ccaattagtg attgattccc ctgttccata
    15181 tttcattgcc gggttagcag atacactagc aaaatggtat gaatctgagc tcatcttaga
    15241 acaagatttt ttgcaagatg aatcattttt gaagctagca caagatactg cgaagatctg
    15301 caaagaagag attttgaatc attctgctaa agcaatccaa gatatggcac aaagaaaact
    15361 gacatcggag ttcaaacagc tttcagagat tatctttgct gttgctggtt tagttggagg
    15421 attaggagat aaatacgcaa gaaatgcggc agcacatgct atgcatgtcg ctatcagtaa
    15481 atatattccg gaaagccatc gttttctaca tggagaaaaa gtagcgtatg ggattttcta
    15541 tcaactggca ctagaaggtc gctgggaaaa aatagacgag ttatatcctt tgtatcggga
    15601 attagaactg ccggtttctc tagaacaaat gggattattt ccgaaagata aacagataat
    15661 agataaaatg gctgaattta ttgattcgaa agagaaagtg catttgattc ctatagaggt
    15721 aacacagaaa tcactgatac agtcaatgat ggaactggaa ggttatatca gaaagatctg
    15781 aaggaggagg acacatggaa acattaagtg tagtacaaag tttgttgatt gctttatggg
    15841 tagccgcggt tatgtcccgc tggttaggtg gaggagccac gttgacctta cgtttttctc
    15901 ctttgatgac tggattagtc gtcgggttga tcatggggga agttgcacaa gcgatgattg
    15961 ttacagccgc tttgcaaatg atttatatgg gggttttttc tccaggagga tcaatgccag
    16021 ctgaaccatc tattgcagca gctatcgcag taccagtcgc attattaggg gatctgtcgc
    16081 cagaagcagc gattgccgtt gcggtgccag tcggattgct cggaagttac ttgtatcagt
    16141 ttcgtttttt catcaatact tttttaggta aatatacaga caaagctgta gaagatttga
    16201 atacaaaagc tatggcccga tcaattattc tttatccaac ggttgcctca tttttattat
    16261 tcgttccgct tgttttcatt gctttatatt taggggcgcc attgatcgct gatgtaatca
    16321 aagctttaga gggaacagtc gttatccaca ttcttgaagt agtaggtgga gggttagcgg
    16381 caatcggtat cgcgacaact gtatacgtta tcggtcgaaa agattatctg gtctttttct
    16441 tccttgctta ttttatgagt gttgttttga aatctttaga tattacgatg gttacttatg
    16501 ccatttttgg gattattatc gctttgatct ttgtccagtc gcaaaaaggg aaacaagtac
    16561 agcctgccac aagcaataac aattcagcat cttttgatga cgaagatgac tatgatgacg
    16621 gattttaatt taggaggaat gggaaatggc tgaaataatg gataatcaaa caggtgctcc
    16681 tgaagaaatc acgaaaaaag atgtaacaaa agcctatctg agatggcatt ttgcaaacga
    16741 aattcctcac tcttttgaac gttacttggc gccatcacta ctatacggaa tgatgccaat
    16801 cttgaaaaaa ctttataaag atgaagactc tttaaaagca gcatataaac ggcagttact
    16861 gttcttcaac actcaattaa gctggggtgg cggtgtgatt actggcctaa tgtcttctat
    16921 ggaacagcaa agagcagaag aagagtatga agaaaaagag atcttgatgc aggatgactt
    16981 gatgtataac acgaaagcgg gattgatggg ggcactagca ggaatcggag atgcaatcga
    17041 ttcaggaacc gtccaatata tttttatcgc aatcgcggta ccttgggcac aacagggaag
    17101 tcctttaggc gcactttttc cttttatctg ctttgggttg tatcaggttt tactaggggt
    17161 atttttcgca agacaagctt tcactatggg ccgtaatgct acaggcctaa tgcaaaacac
    17221 tggtgttcag acagcgattg aaatgctatc agttttaggg ttgtttatga tgggggtgct
    17281 tgcgggaaac tatgtaaaag tagaatcttc attgaaattt gcgatatccg gacgtgagtt
    17341 tgttatccag gaaattcttg atcaaatcgt gccagggata ttacctttag ctgtcgtaat
    17401 gggtgtgtac ttgttctata cgaaaaaagg attgaaagtc acacaagcat tattatggct
    17461 gactggaata ttaattgtgc tagcaggaat cggtatttta taaaaagaag ggaacgtgaa
    17521 taagatgggt gtagtcaatt tagcgcgtgt ggatgagcgt ttgatccatg ggcaagtaat
    17581 ggtaacgtta tctcaaaaaa gtggagtgaa ttccattttt gttgtagacg aagttgtagc
    17641 aaaagataaa tttatgagag atctttacaa aaatgcagga agccgtacag gacaaaaaac
    17701 aattgtcatt acaccagaga aagctaaatt ctattgggat gaatatcaat ttaaagaata
    17761 caattgtatt ttgatcgcaa aaacagtcca aacgatttac gacttagtaa aacacggaat
    17821 ccccatggaa gaattgaata ttggagggat tgctcaaaaa aatcctgata cagatctttt
    17881 agtgacgaaa tctgtttatt taaataaatc agatgctgaa aaattgaaag aaatgaatga
    17941 acaatatagc gtaaagaata tttattttca agccactcca tctgcaccta aaacaggatt
    18001 aaaagatgta ttgaacaagt ttgatttgtc ataaacaaat gcttagaaaa aagataaaga
    18061 gggcgaaaaa atgaagaaga acgagctgtt tcgtgattgg gaatttcgtt atcgctatat
    18121 ttaccgtaag cgccgtacaa aaaagagtaa acagcgtttt ctttcagcat tagtatcaga
    18181 tatttacagt atgagaacag atgtgacagt cattgcttac gatacgctag cctaccgatc
    18241 aaaaaatatt tatgttggag atatcgaaaa agcagagaaa gttatctgta cttattacga
    18301 tacacccgtt catgcattag gaagttattt catgtttgat tggaaagacc aaaggaaaaa
    18361 aacaatctac tctatattat tgtccttcat tttgttgttc agtttaggtt ggtggggaat
    18421 gatgatatat aacaaaaatc ctcatcacgt attcgatctg ctatccgtat aaacaggtat
    18481 cactgtacta gcatttggga gctacttttt cttattggga aaagcagctc gcggatggcc
    18541 tagcagacaa acttttattc gaaatacttc atccatatta acgatgctag aaatgatccg
    18601 tacgattgat gatccgagtg tagcgtatgc gtttgttgat gaaggctgtt atggagaaaa
    18661 gggcttagac agcgtgagat cgggtatgaa aaaagaggct attctttttt atctagactc
    18721 tgttggggca gatacaccgc tacagttttc gggaaattat ttttcaaata aagaacagtg
    18781 gctgaaacaa gttgataagt tgaaagaaaa aaacgtcaat tatattttta gtgcaagaaa
    18841 aaagcaagcg cagttcttct atttgaccaa aacagattta cgagggaaaa catttaattg
    18901 gcaaaatgcc aatcagatta ttgctctttt tcgataagta aaaagtgcaa aacaaggagt
    18961 gagaagacta atgttggaaa tcatgatcgt tacacatggt aagttgagcg aaggactgaa
    19021 agatgcggcg gaagtaattg tcgggacatc gagccatatt gaaacaatgt gcttagagtc
    19081 tggggaggac atccaaacat tcggagagaa ggtcaaagaa aaattaacta tgatcaacac
    19141 agcagaaccc atgctagtat ttgttgatct aattagcgca agtccgtaca atcaagcgct
    19201 cttggcagtc aatgaactga ctgaagaaaa gaaagcagcc acttatgtca tagggggcgt
    19261 gaatctgcca atgttgttag aagcaatcaa tcatcagatg cttcacacac cgatagaaga
    19321 agcggtggaa caaattgtca aacaaggaaa agataattta actgtttggc atgcttcagc
    19381 ggaagaaaaa aaattagatg aggacgattt ttagaaggaa ggctgaaaag atgaagtggg
    19441 gatttagatg gtatggtgaa aaaggggatt cgatcccatt aaaatatgtt cgccaaatac
    19501 cgggaataaa tgggataatt ggaactttgc tgactaaaat gcctggagat gtttgggaga
    19561 tttcagaaat tgaggagttg aaagcatctg ttgagagcca aggactggaa ctgcttggaa
    19621 ttgaaagtgt agcgatccac gatgcaatca aggcagggac agatgaacga gattactata
    19681 ttgataacta tatacgaaca atcaaaaact tgtctcgatg tggggttcga ttgatttgtt
    19741 atagcttcaa accaattttt ggatgggcga aaactgattt gtttaatgaa aacaacgatg
    19801 gaagctattc tcttttatac gatcagaaaa tcgtcgatca gatggagcca ggagaaatgt
    19861 atcaattgat acacagccag tcaaagggat tcaaattgcc gggttgggaa gaagaacggt
    19921 tgaaaaaatt cgctcagctg gaaaaactgt atgaaggaat gaccgaagaa gaattatttg
    19981 aaaatctgac ttatttttta gaaaaagtga ttccagtgtg cgaaaaagca gatgtaaaaa
    20041 tggctatcca tccagatgat ccgccatggg aactttttgg ttttcctaga gtgacgaaaa
    20101 atcttggcga cttgaagaaa attctaaatg cagtagattc tccatataat ggactaacgc
    20161 tgtgcacagg atctttaggg gcaaatccgg aaaacgattt agttgaaatc atccatgagg
    20221 taggttcgcg catccatttt gtccacttca gaaacgtgga gtttttagga gaaagaaagt
    20281 tcaaagaaac ggcacatccc tctacagaag gctcccttga tatgtatcat attatgaaag
    20341 cgttggtgca agaagggttc gacggtccta tacgccctga tcatggtaga accgtgtggg
    20401 gtgaagtggc tatgcctgga tatggcctgt atgatcgtgc aatgggactc acttacatgc
    20461 aaggtctata tgaggcgata agtaaggaaa aaccactggg agagcattat gaataaaatc
    20521 aagctcttca tctttgatat ggatggcctt ttattagaaa ctggacgttt agcttatcgt
    20581 gcttatgtaa aatcagcaca aaagtatgat tatgaaatgc gtaaggaagt ctattattta
    20641 ctaactggcc aaacagaaat ggcgataaga gaacaaatgg gcattttgta cggagaagat
    20701 gttccatata tcaaatggcg agaagcaatc aatcaatata aagaaaaaat cgtgaaagaa
    20761 gataagagag tatatacgaa aaaaggagca gaagaaattt tatcttttgc aaaagaaaga
    20821 ggaatccata caatcgtggc ttcgtctaat acacgggtaa aagtagaaat gtatttaaaa
    20881 atggaaaatc tatatgcctt gtttgatcaa atcatttcag gggatgatgt aaaaaaagga
    20941 aagccggaac cagagatctt tttgaaagct tgttcaaaaa tgaatattcc tccaagtgaa
    21001 gcacttgtat ttgaagactc gattgcaggg atcgaagcag caaggcgagc gggcattttg
    21061 tcttttctca tcaaagatca tttgataggg ttgcccgatc ataatgggaa gcataagttg
    21121 aaagtagatg taagtttata tgaatccaat aaagcagact atgaatttta tgacctgcat
    21181 caagcaaaac gatttttaga aagtaaaggg ttgtatctct aataaagagg actattttct
    21241 agtcctcttt atttattgtc ctgcacgact gagggaacag atgaaaaaga agtaattttt
    21301 tattttgaag tatctacctt atttgtagtg aacaatttag aacaaacaca aaacgcatct
    21361 actgttatct accagtagat gcgtttaaag agaagaacga gaatcgtcca tatgagggtt
    21421 tccactcctt atgcagattt ttttaattct tgcactgctt gttcgatcgt aacacctgtc
    21481 gctacatgat tggtattttc tttgtcaaaa acgacaaata gattcaaatc tgtatccaag
    21541 gttactcgat attgtatatc tttgttatag aatgtcatag ccattctcct ttctttaaat
    21601 acttgctgaa aggcaacgaa cgcgttgata acgaaattcc atttataagt ataccaccaa
    21661 ttgaaaggtt ttcatagtta agaaatttta aagtcaaaag ttgcagataa aagcaaataa
    21721 acaagaaaac aaaaagtgaa tcaatcgcgg ttattcaact ttttcttcga ttcgaagaca
    21781 atagaagaca atgaatttta tatttcattg cattatcctt gtgttattag aaaaagcatg
    21841 atatattaaa tgagtaatct attttgaaac gtaatttgag cgatatatct acatagataa
    21901 aactcctttt gcctatcaaa tatttattgc aacgaaaaaa cgtacattga tacgtatcag
    21961 gaggaattta tttatgaata acggtacagt aaaatggttc aacgcagaca aaggttttgg
    22021 ctttatcact ggtgaagatg gaaatgatgt atttgcacat ttttcagcta tccaaggtga
    22081 cggcttcaaa actttagaag aaggccaagc agtgtcttac gatgttgaag atggtcaacg
    22141 tggccctcaa gcaacaaaca ttgttaaagc ttaataaaaa ttgactttta accgtctttt
    22201 cgaaagaaaa gacggttttt ttgtacgaaa aaattcattg gtcgttgaat gggctgaata
    22261 gtagtgtata aaacaatttt gtggagcgtg aagcttttat ggtagaggaa aatacgaata
    22321 aagtgatgtt gataaaaaga ttcctatctt gaatatgaat cgagatgtct tgaaaatgaa
    22381 tgagtttgac caatcattat cctatcgaaa tttacagttt cgttcgattg gtctgtcaat
    22441 taatgaaaaa gtaaaaaaga atatgttcta ggaagagggc ggcacatcta aatttttaag
    22501 taacggacag ttcaaatatc aagcattgaa agaggctatc ctactgtttg aaggaatacg
    22561 aatatataaa aacgacaaag aggaaaaagt agttcattaa atcccttcaa gtgatgaaag
    22621 aatgagatct aataaaacct gatctttcat ctgttttttt aataacgtaa taattttttt
    22681 attttttgtg ctttcgccgt tattgtagag tgatttagca ttcttatttt taaaaataac
    22741 tgtgttatat attaaaaaag gcataatagt tataagcttt tttaaagaat atttagaaat
    22801 tattaatttt cgtctgaaaa caaaaaaaga aagcgttatt attttagtct attttcaggg
    22861 ggggaacctt atgataaaaa aactattttt actatcaata ttaacgctag caatggcagg
    22921 ttgttctgac tctggcttag caactgaact gatcacaaaa tcaactgaag ccacgacaga
    22981 ggagttaaaa tgtggaaacg atcacagtga ctacagaggc ttaaatcgac gatgcatggg
    23041 aatattaggc agaagcaatt gtaagcatcg atgtagtaat cgaagaaggt gtagaagaag
    23101 caacagaaaa atcaaaagaa agatcaaaag aagcaatctg tgctgtgcat cgtatacaga
    23161 cgtcgaagat gcggtacttt taaaccacgt caggagatgt tcatttatgt ctagggaagt
    23221 tggcgatgaa ccaaaactag agtacgcttt tagttttgca cgtgaggatg aagataaccc
    23281 taaaacttac gacattttag cttgatctaa gatggttaaa gctttttaat gaagtaagag
    23341 gagatagaag gcttttctag ttatattagg taagataaat tcctttttgc tccaaatttg
    23401 ctaaacattc tgctagataa acttcatcgt gttccggata aatcggcacg ttgcgctcta
    23461 cagtaggtaa atcaaaaaat gcttcgcatg atttgccacg atacatagga tttttccact
    23521 ctggtgctgg gctgtaacca cgtttttcca tttcatccat gacaagctga tgatattgga
    23581 ataatctata aggagaatag tgaaaaacat aatccacagt agcatgtttc tttccccagc
    23641 catttcctct taatgcacag cattcccgat gttgtcctaa aagctgttga cgaggaagct
    23701 ttgaaatcaa tttttcatgc cataatcgca tagaaatgtc tcctttatat atagattgtt
    23761 aaccttagac gttaaatgaa aaaaccagca ccaaggctgg aaaaaatcac ttcagtttgc
    23821 ttgctgtttc tgagcgacca attaggtaat caacggaaat atcgtaaaag tcagcgatat
    23881 agattaattc aatgatgctt ggaatttctt ctccttcttc gtacttacga tatttgaata
    23941 aaggaatact taacgcgcta gaaacagcta cttgagataa agatcggcca gttcttaaga
    24001 aattcagacg acaacgtaaa gtatccagta aaatagcttc ttcgatactc atttaatcac
    24061 ctttcattat agtagaatag ttataatgac tatagaaagc tacacatgca atcaatagta
    24121 ctcaatgttt tttatttaaa cgtatcaaat aaagaatgac gatcaataaa gacaaaacca
    24181 gtcctgataa ttcttctata aaaacactat cgggttcaaa gtagttgtaa ttaaaaactt
    24241 tccacataaa gtagatagcc ggaagagtca aaatagctaa ataaaaccgt tctttgttcc
    24301 aattcataag ataaacaata cttgcgacca gtgcgaccgc aggcacccaa aacaaaactc
    24361 tcaccaacgt aatcatgtaa cggactcctc aacgattcat ataataaaag ataacatgtt
    24421 attgcataca tcacaaatga aatgctagga aaataaaaga agaatagatc aatgaaaaat
    24481 gaaaaagcag aacagttttc tgctgataag caggaactgt tccgctgaat gtgtcttatc
    24541 tatcgagaaa aaatcaatgg attgatatct aagtaattca atacatataa aaagaggacg
    24601 atcgcattca ctaaaaagga aagaatgatg atcccatagc gaatggaaac atatttaaca
    24661 aatacttttg tttgaatacg ggcatatctt tttcctttat agctgagcat catgacaaga
    24721 agcaaaaggg taatcacacc ttgaaaaatc attccaataa acagaattcc tgaggtttga
    24781 taatttaatt ggagaaaata agctgctgcc gcaactaaat cgaataaaat cgcaataaat
    24841 ggcaaaataa tcactcctaa cattgagaat aaataaaaga ataacatttt ttctaaaata
    24901 agcaaataaa aattaaagta acaaaatagt tatgacacaa gtaaaagaaa taagaataac
    24961 aatattattt tacttatatt ttgaaacttg tgagagtttg tcattcgtgc tatagttgaa
    25021 aagtaacaaa aataaatttt atttatgatg tacctttagc tcagttggtt agagcagacg
    25081 gctcataacc gtccggtcgt aggttcgagt cctacaaggt acataagagg ttgtgaaaaa
    25141 gctgccctgc atcaagtaat aagaacagcc aagtaaaaac agcttcttat gtttttactt
    25201 ggctgttctt attactgcag atgcaagctt ttgaataccg tttaaggaag aggttgtgaa
    25261 atcagcgttt tgacctgagt attagcttaa caaacggaaa aatagttcct catattttgc
    25321 cgtttgttaa gctaataccg agggtcagct gattgaacac catttaagaa agaggtcgtg
    25381 agcctgccgt ttcactctgt gtcataaaaa agccaatcta ctttgataga aaaagcacgt
    25441 aggactaaaa cagttctgcg tgcttttttt attgtgtgtt aaatatcgta aatgaagata
    25501 gatattttta tgattcgacg atagagacgt tgaaatcttt caacagctct aaagcggatt
    25561 ctccgtgttt tctactggag actaacattg gatcaacaaa aaagtctgct tcaggaaaag
    25621 cactctgcag gcctatcaga ttagagagta cacacaagtt tgtttcaatc ccgactactt
    25681 cgatctcttt ttgttcttct ttgctttcaa ataaagtttc ctggatttca gctaaagttt
    25741 caggtggaat cgtaaagtaa tactttttaa tcactcgttc attttcttgt ggagaaagaa
    25801 gagggatcaa ttgcaaatct tctcgttctt cttctttgtc cttcagttca actggtatat
    25861 cccgtgtgaa gagaatatat tcattatttt ggcgtgcttt agctaatctt tgagcaatac
    25921 gttctgccaa ttgatctgac tctggaatat aaaattcgct gtttggatca agaatatgat
    25981 tttgcatatc aataataact aacattttct ttcctcctac attcatatcg tcattttatt
    26041 ctttttaata gaagtttttt aaaatataag caacaccagc ttcttcattc gtcagtgtta
    26101 cttggtttgc aatttctttt acttgtcgct gtgcattccc catcgcaata ctcaacccgc
    26161 tgtaattcaa catagcaatg tcgttttctt gatcgccaaa agccatgatg tcactcgatt
    26221 cgattccaag gtaatccgcc agaaaaacaa gtgaatgagc tttagtgata cctgtaggcg
    26281 tgatttctaa gaaatgcgga tcagagaata ctgattggat accatctgct tgcagatctc
    26341 tttgtagctt ctgtagtttt tcttcactct tatctgttaa tgctaattta agatagctaa
    26401 gagaaggatc ttccaacata tttaaaattt gatcaaaaga atggttctgt aaattgaaat
    26461 gttcataata agggtcatct ttgactagat ttgattcatc gaatgtcagc tgatcatctt
    26521 gggtttctcg gctagcaatg atggatattt tcgcttcttg agcatagaag aaggcttttc
    26581 tcagtgtatc gctatctatt tttgaacgtc tgagaacttt cgccattttt ccatccttca
    26641 tttcagcaat cagtgaacca ttggaagaaa tcaaatagcc accgattcct aattcggaaa
    26701 aaatatcttt agcagaaatg ctgttccttc cggtagcgac ggttacgatt ccgccatttt
    26761 catcaaattt ccgaagtgct tggatatttt cttctggtat ctttccattt cgatcaagca
    26821 aggtaccgtc taaatcaatc gctgctagtt tcattttatc ttcctacttt ctttttacat
    26881 atctattttc ctgttacctt aagattaaat gagaatttaa aaaaatgaaa ctaacttgct
    26941 ttgtcattat gaattttttc aaaaaacatt cttaagaaaa aggtggctat atatagtagt
    27001 ttttggtcaa tacctttgaa taaaaagcca tgaaaaacga atagttagcg cttaccgatg
    27061 cgttttgtga taaaaaaatt ttaaaatatt caatgaaaac aaagcatata ttttgcttta
    27121 aaaccaaatg aggaatattc tagatgtaga acaggttaga aaaagattag aaaaaggagt
    27181 gattttgatg gagaagagat acggcgtagt tcctgtggta actgcttcag acgaaaatta
    27241 tgctccttac ttgagcgtga tgatcgcaac tgcattagaa aattgcaata aaacaagacg
    27301 cataaaattt tatgtgattg acgatggtct atcagaatat agtaaagaag gattggaaga
    27361 aacggtaaat aaatattcaa gtaatgcaag tatccaattc ctgacagtgg aaaaagatat
    27421 ctacgaagac ttcctagtca gtgaccatat cacaactacc gcttacttaa gaatttcttt
    27481 accaaactta ttagctaaag aggattataa gaaagtcctt tacttagatt cagatgtgtt
    27541 agtactagac gatatcgtaa aactatatga tgagccgtta aatggaaaaa caatcggcgc
    27601 tattatcgat ccaggccaag tgaaagcttt ggaaagatta ggaatcgatt ccgatgactt
    27661 gtacttcaat tcaggagtga tggtgattga cattgatcaa tggaataaaa aagaaattac
    27721 agaaaaaact atccattacc tcagtgaaaa tggcgatcga atcatctatc acgaccaaga
    27781 tgctttgaat gctgtacttt atgaagattg ggaacaactt catccaaaat ggaacatgca
    27841 gacttcgctg atttttgaac gtcatccagc accaaatgaa aaatatgaac gtcagtataa
    27901 agaaggaaat gaaaaacctt cgatcgttca ctttacagga catgataaac cttggaatac
    27961 gttgaaagat catccgtata ccaatttata cttaaaaaaa ttagcacaca gtactctaac
    28021 gaaagtaggc gagatcaatg aataaaaaag aaatagcagt agtcgcaagt tgtaacacaa
    28081 aattcgtacc acatttagct gcattatttg tctcagtatt agataattgc aacccgtcta
    28141 aatttgttcg gttctatgtg attgacgatg atatcgattt tgaaagtaaa cagcttttac
    28201 gcttctcagt gaaaaacgca cgaatgaact cagatgtgga attcttgaaa atcaataaag
    28261 aattctttac aaatgtggta atcagcgatc gtattccaga aacagcttat tatcgtattg
    28321 cgattccgga attgttccgc ggcacagaag ttgaacgtat cttatatatg gattgcgaca
    28381 tgatcgcttt acaagatatc tctaaattat ggcgtcttga cttcggtgat tcaatcgtag
    28441 cagctgttga agacgctggg ttccatcaac gactggaaaa aatgaaaatt tcagctaaat
    28501 caatgcgtta tttcaactca ggtttgatgc tgatcaatgt gaaaaaatgg ttagatgaaa
    28561 atattacgca aaaagtatta gatttcatcg aacataatcc agaaaaatta cgattccatg
    28621 accaagatgc attaaatgct atcttacatg accgttggtt accacttcat ccgcgttgga
    28681 atgcacaagg ctatatcatg gcaaaagcaa aaaaacatcc aacagcagca ggtgaacgtg
    28741 aatacgaaga aactcgcaac aatccttaca ttatccattt ttcaggacat gtgaaaccgt
    28801 ggagcaagga ttttgaaggg ccaacgaaga aatattacga aaaatatgca gggatgacgg
    28861 ctttccgttg tgtagctaaa ttccctaaat atccaaaata tgcaaaagtt caaccaaaaa
    28921 cagatgtagt tgttaaataa tagaagacaa caactgaaga gggggagtag gaatggaaat
    28981 tacaatgtct aaaaatgcag tagagacgtt aattgaaaag gttggggaaa atacaaaaat
    29041 cgcactcgcg cttatcaatg attccgatcc atttctaagg gacaaaggtg cttttgcaaa
    29101 aggcagcttt tttcaaatca ttccttttgt ttcggaattt ggagaatatg cgactaaaat
    29161 cgagcatcca ttattggaca tctatacatc taaattagag caaaattatt tcggtaaacg
    29221 attgaacatg gacttcaaca aacagcttga cagcttttca ttagaaaatg aaatcgcagt
    29281 actcgattat aatatcaaat taaagaactg cttcttcagc taatagtttc gagaaagtcc
    29341 aaaaaggagg tatacattga gatactactt tgtggatgaa gaaaagaatg taatagaaac
    29401 agatgaaaaa aaatataatt ggctcgtact tgatagtgca aagcccgaag agattgagca
    29461 agcaatcgaa gaattcgagc ttcctgatga tattttcgta ggaaccgaat atccggaaga
    29521 agtttctcgg atggagcgcc tttatgagac gaaactagag catccatttt ctttggtagt
    29581 gattaatcta gatcagtcag ataatcgtat cgaaaaaaat taacgccaat atcttttgta
    29641 ctttccgata atctcttgat tacttgtatc gatagaaaaa ccgaatttgt ggatcggctg
    29701 ctgacaaagc atggaaatca tttaaactcg tttgaaaaga tcatcacgta tacattatta
    29761 gatatttaca cccattacgt gaaagaacta agagagatga aaaagcgaat tgacgctttg
    29821 gataaagcag ctcgtaaaac aacggaaaat gaagaactgt ataaacaggc agatcttgaa
    29881 agagacatcg tatatatcga tcatacctta agagatcaac gggaaacaat ggatcaatta
    29941 tggaattctg aaactttcat caaacgttta gacgatgaac agctactcta tgatgtccat
    30001 ttgagacagc gacaaacaga aaaaatgatc gagatctatc gagatttatt agagagtatt
    30061 ggtgatctat tcaacgggat gatggacaat aatctaaatc atatcatgaa atatctagat
    30121 tcagctgcat taatcatttc cgttccggcg ttgttcgcag ggatttgggg aatgaataca
    30181 ggaggattac cgggtaaaag ttctgctcta ggctttatac tcgtagtctt tggctctttg
    30241 atagcagcag tagttttagg ttaccattta taccggaaag attatacaaa ttaaagaatc
    30301 tatttaaaac aggtatcaaa caaagaaatt tgttttggca atacaattta aaaactagga
    30361 ggaatacagc aatgacagaa ccaaaattaa tcgacccgcg aaacttgtac catacggaga
    30421 aatttcctaa acaagatcag gatacaccag ctttacagca gaatatgatt cctgttcctg
    30481 attgtggaga agaatcctat gaaggcaata atcaactaga aaaccgccgt gtactaatca
    30541 ctggtggaga ttcagggatc ggtcgtgcag cagcgattgc atttgctaga gaaggggcag
    30601 atatcgctct tcaattcttc ccaggagaag aaaaagatgc agaggaagta gcaaaatacg
    30661 tgaaagttgc aggacgtaag attgtcttac taccttttga tcttcgtgac gaacaagcac
    30721 ctaaagaaat catagataaa gccgtgagtg aacttggcgg gctagatacg atggtactaa
    30781 acgcaggaca gcaaatttct tgtcagtcta ttgctgagtt accacttgag caagtcaaag
    30841 acacatttat gataaacatc atcgctatgt ttgctttagt gaaagaagct gttccgcata
    30901 tcccagcagg aggagcaatc gtaacaacaa cttctgttca agcattcaat ccaagcgaac
    30961 atttattaga ttacgcagca acaaaagctt caatcgctaa ctttacgatc ggtttagcaa
    31021 aacagcttgc acctaaagga atccgtgtaa atggtgtagc acctggtcca atctggacac
    31081 cattacaact agacgaagga caaccttctg attcgatccc tgaatttggt cagcaaagtt
    31141 tactagaacg tgcaggtcag ccaggagaat tagcaccagt ttatgttttc ttagcatcga
    31201 ataaagcaag ttatgttaca gcccaaattt acggagtaac tggtggagaa gcgatcaact
    31261 tataagaagt tgatcgtttc acccctatga ggaggtgcga aaatgaataa aggtgtaaaa
    31321 acgatcggag tgatcggaac attacttttg ctatctgtgc ttttgtttgc gatactatta
    31381 agtgcaacct atccattgcc ttttcagcta gagaagtttc gattcttttc catcactaat
    31441 tcatacgtgc aacaatatgt attttggctt gccgttgcat ttgctatttt gacaattatc
    31501 acgttacttg ttttcctgtt ttatccaaaa acaatcggaa actttctact gaaacatggt
    31561 gatggggagt tgacgttgga taaaaaagca attgaaggaa tggttcgttc tcacttgcat
    31621 gaagatgaat tcatccattc accaaaagta aatgtcaaag caacgaaaaa caagatcgcc
    31681 gtcaatgtaa aaggggagtt gaaacgaact tcagcactca ttggtaaaac aggaacattg
    31741 atgaaagaaa tcgaaaatga agtacaacac atattgggaa caaaagagcc agtgaaagta
    31801 tcggtgaaat acacaggctt tgaagaacca gctaaacgaa gcaatgaccg caaacgagta
    31861 gaataggggg ggaccaaaaa tgaaagaact attttcgaca tacaagctcc ctatcatctt
    31921 tggggtactt ggattgatat tagccgtttt atggatcacc gtgggctttt tcaagacctt
    31981 attgatcttg atcatggcag cgatcggcgt cgctatcggt ttttatctga aacaaacgaa
    32041 aatcttagat cactatttta aatcttagga ggaaacatcc atgaataaca ataacaaaaa
    32101 taataacaat aaaatgaaca accaaaaacc agtgtctgca catccagacc caacgacacc
    32161 aaaccaatca aatggtgtga acgatgaaaa gaacaaaaca gcaccagtat ctgcacatcc
    32221 agatccagta gtccctaacg ttcaacaagg agtagaagag aagaaacatg atgcagaaaa
    32281 gcatgcacct catcatccag gaactccaga atctaaagtt ccagaacaac atgatgcaat
    32341 cgatccttct aaacctgtgg aagagcaaaa acatccttct gaccatgcga aagcacaaga
    32401 aaaacttagt tctgaccaca caaacggaca aggaaaactt ggttctgatc atacaaataa
    32461 ccaaggaaag catgattctg aacaggaaga ttcaatgcgc ggtgagctga attatgaaga
    32521 taaagttgtg caaaaaatca tcggaatcgc aattgaacaa gtagacggtt tattatctgc
    32581 aaacggtggt ttcttctcta atgtggcagg aaaacttgtg aatacagata atgtaacggc
    32641 tgggatcgaa acagaagtcg gtaaaaaaca agtagcagtt gatatggatg tcattgtgga
    32701 atatggcaaa gatatcgaaa aaatctttga agaaatgcaa gaagtaatcg gcaaagaagt
    32761 aaaaaatatg acacatttag aagtgatcga agtcaacgct aacgtagtgg acatcaaaac
    32821 aaaagaagaa tttgaaaaag atcaagaaac ggtgcaagac aaagtaactg acgcagcgaa
    32881 aaaaaccggt gaatttgctt cgaatcaaac agataaagta aaatcagcag ctggaagcgg
    32941 cgcacaaaaa gtaaaagaaa atacagaacc acgggttcag taataaggaa gtaaaacatt
    33001 aggaggaacg aaacatggga tttatttggt cacttatcgt cggaggaatt atcggagcaa
    33061 ttgcaggagc aatcacgaat aaaggttctt cgatggggat tattgcaaat gtcgtagcag
    33121 gtttagttgg atcagcactt ggtcaagcat tattaggttc ttggggacca agtattgcag
    33181 gaatggcaat cgtgccatca ttgattggtg ccatcatttt agtactaatc gtatcattct
    33241 ttgctcgaaa actgtaaaaa gacttttaaa aagaggtcgt gaaaaagctg ttcttcatca
    33301 agtaataaga acagccaaac aaaaatagct tctcatattt ttcatttggt tgggcatatt
    33361 atcgaagatg caagcttttg aacactgttt atacggaata agaggctgtg acaagacttt
    33421 tgtcacagcc tcttatttat gcgggcaaat tcccaattag atttttaaaa gtttttcctt
    33481 aaatgtgtct ttattgatac agagaagctt ctttaataaa acaatgcctc cctaaatttt
    33541 cttgagatag gaaaggtgaa catacaaaat aatacgacca ctttgaaaac ccagatgatc
    33601 cacaaatgga tcgtatgata ctgatgaagt aaaaagcaat ggatcagaaa ctcaaagaga
    33661 ttgaaaaaga caaggaagaa ataatatctc ttttttactc ttgtctgttt tagccaataa
    33721 tagctcgtaa agtaatctag acaaacaaat agaagtaaac aaagtttcgt aagagcagca
    33781 atcgtatcat gttgctctgc tagacgattg acgagcagga aaaatgttgg taatggaata
    33841 tagatcacga gtaatgaaag aaaaaagtaa acaataaatg gcatactatt cctcctaatt
    33901 gtgtgagttc gccctcattt taaaataacg atatttgttt gtgaagcttt ttcgtgtcgt
    33961 aacaaatgtt atctaatgta tttttaaata aataatcatt attatcaaaa taatg
//
LOCUS       DACECY010000028        32800 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.029, whole genome shotgun sequence.
ACCESSION   DACECY010000028 DACECY010000000
VERSION     DACECY010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.029"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..335)
                     /gene="rpsI"
                     /locus_tag="IWO38_001413"
     CDS             complement(<1..335)
                     /gene="rpsI"
                     /locus_tag="IWO38_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ0313508.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMV"
     gene            complement(351..794)
                     /gene="rplM"
                     /locus_tag="IWO38_001414"
     CDS             complement(351..794)
                     /gene="rplM"
                     /locus_tag="IWO38_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ0313509.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(1313..1780)
                     /locus_tag="IWO38_001415"
     CDS             complement(1313..1780)
                     /locus_tag="IWO38_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ0313510.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(1900..3438)
                     /locus_tag="IWO38_001416"
     CDS             complement(1900..3438)
                     /locus_tag="IWO38_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ0313511.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            3518..4204
                     /locus_tag="IWO38_001417"
     CDS             3518..4204
                     /locus_tag="IWO38_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ0313512.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(4405..4932)
                     /gene="msrA"
                     /locus_tag="IWO38_001418"
     CDS             complement(4405..4932)
                     /gene="msrA"
                     /locus_tag="IWO38_001418"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0313513.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(5016..5693)
                     /gene="pgmB"
                     /locus_tag="IWO38_001419"
     CDS             complement(5016..5693)
                     /gene="pgmB"
                     /locus_tag="IWO38_001419"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ0313514.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(5690..6820)
                     /locus_tag="IWO38_001420"
     CDS             complement(5690..6820)
                     /locus_tag="IWO38_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0313515.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(6820..9003)
                     /locus_tag="IWO38_001421"
     CDS             complement(6820..9003)
                     /locus_tag="IWO38_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ0313516.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(9000..10025)
                     /locus_tag="IWO38_001422"
     CDS             complement(9000..10025)
                     /locus_tag="IWO38_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0313517.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(10070..10897)
                     /locus_tag="IWO38_001423"
     CDS             complement(10070..10897)
                     /locus_tag="IWO38_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0313518.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(10894..11961)
                     /locus_tag="IWO38_001424"
     CDS             complement(10894..11961)
                     /locus_tag="IWO38_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ0313519.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(11987..12823)
                     /locus_tag="IWO38_001425"
     CDS             complement(11987..12823)
                     /locus_tag="IWO38_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0313520.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(12820..13689)
                     /locus_tag="IWO38_001426"
     CDS             complement(12820..13689)
                     /locus_tag="IWO38_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0313521.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(13759..15042)
                     /locus_tag="IWO38_001427"
     CDS             complement(13759..15042)
                     /locus_tag="IWO38_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0313522.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(15056..16741)
                     /locus_tag="IWO38_001428"
     CDS             complement(15056..16741)
                     /locus_tag="IWO38_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ0313523.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            17016..18041
                     /locus_tag="IWO38_001429"
     CDS             17016..18041
                     /locus_tag="IWO38_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0313524.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(18636..22289)
                     /gene="rpoC"
                     /locus_tag="IWO38_001430"
     CDS             complement(18636..22289)
                     /gene="rpoC"
                     /locus_tag="IWO38_001430"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ0313525.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(22368..25985)
                     /gene="rpoB"
                     /locus_tag="IWO38_001431"
     CDS             complement(22368..25985)
                     /gene="rpoB"
                     /locus_tag="IWO38_001431"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ0313526.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            26713..27681
                     /locus_tag="IWO38_001432"
     CDS             26713..27681
                     /locus_tag="IWO38_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0313527.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            27699..28604
                     /locus_tag="IWO38_001433"
     CDS             27699..28604
                     /locus_tag="IWO38_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0313528.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            28601..29407
                     /locus_tag="IWO38_001434"
     CDS             28601..29407
                     /locus_tag="IWO38_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0313529.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            29564..30532
                     /locus_tag="IWO38_001435"
     CDS             29564..30532
                     /locus_tag="IWO38_001435"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ0313530.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(30849..31373)
                     /locus_tag="IWO38_001436"
     CDS             complement(30849..31373)
                     /locus_tag="IWO38_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ0313531.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            31660..>32800
                     /gene="scm"
                     /locus_tag="IWO38_001437"
     CDS             31660..>32800
                     /gene="scm"
                     /locus_tag="IWO38_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ0313532.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDST"
BASE COUNT         9196 a         7075 c         6185 g        10344 t
ORIGIN      
        1 tcgaccatac gtgcgtcacg agtaagtaag ccagcacgtt taagagctaa acggaagtca
       61 ggatcaactt ctagcaatgc acgagcgata ccatgacgga tagctcctga ttgtccagcg
      121 tatcctccgc cgtttacgtt aacaaatacg tcatatgcgc cttttgtttc agtaacagcg
      181 aaaggttgat tgattacttc acgcaagtca gcatgtggaa tgtattcttc aacatctttt
      241 ttatttacag taattttgcc agttcctggt actagacgta cgcgagcaac tgcgttttta
      301 cgacggcctg tgccgatata ttgaacttgt gccaatgaaa ttccctccta ttagattaag
      361 tttgtgatgt ctaaaacttc tggttgttgt gctgcatgtg gatgttctgc tccaccgtat
      421 acgttcaatt ttgtaaattg tttgcggcct aaagtgtttt taggaagcat accttttaca
      481 gaagtttcga tcaaacgacg agaattttta gcacgcaatt cgccagcagt gattgatttc
      541 aatcctcctg ggtagttgct gtgacggtaa taaattttgt cagtcgcttt tttacctgtt
      601 aatttcactt tgtctgcatt gatgacgatg acgaaatcgc cagtatccaa atgaggtgtg
      661 aaagttggtt tatttttacc acgtagtaca gatgcaacaa ctgttgaaag acgtcccaat
      721 ggaacatcag ttgcgtctac tacataccat ttacgttcta cttcgcctgg tttggccata
      781 tatgttgtac gcacgttatt ttcctccaat ttaagttctg atgtttgaca tacacttcga
      841 gtttccgggg ctctttgtgg ggcaaacaat accatttaac atattacccg ctaatctagt
      901 caatgtcaac gtttttctgc ttgattgaaa aatttatatg aaactttact tcttttcttt
      961 cttttatata aaagggtcgt ttcctgtcaa aatgaaaaac aaaaagaggc tgtgacaaaa
     1021 gtcttgtcac aaccccttat tttgaataaa cggtgttctc ctgccaactc ctcggtcaca
     1081 agtcacaata tccataaaac ataagaagct gttttttaaa attgtttctt gtgactcgga
     1141 gctgaacggc aggcttacaa cctcttggta aacggtgttc aatcagctga ccctcggtat
     1201 tagctcaaaa aacggcaaaa tatgaggaac tattttgacg tttgtttctc taatactcag
     1261 gtcaaaacgc tgatttcaca acctcttttt cggtcaagtt caacaacagt ttttacgcgt
     1321 cgatctctgg cgctttgccc aaatgagctt ggatcatcca gatacgtttt tctgtggctg
     1381 ttttgaattc gataaagata tcttgtgttg gatcgtcgcc ttcttctcca gaaacttcga
     1441 ttcctttttg gtaaagatct gctagataac gatagcctgc aacaagtgtt tccaaacgtt
     1501 ctggaatagt acgatcccag cggccaattt catcagggat ttttgtgtta tctgcaaatt
     1561 cacgcaatgt agaataaggt gaaccgtcta atgtgatcaa gcgttcggat acttcatcta
     1621 actgagcgtt caaatcatcc atgaactcat ccatcattgg gtgcaatgta aggaaacctg
     1681 gtcctctcat gtaccaatgt gtttggtgaa caacaacaga catttggctt agatctgcaa
     1741 ctaattgatt taatacttct ttggtttgtt ggaatttcat ctttcttgcc tcctaacatt
     1801 ttcttacact taaaataata gtagactttc aatgaaaagt acaacaaaat catttacctg
     1861 agaacgattt tcagttcgct tttttatccg taaagccaat catttttcag aatcagctaa
     1921 ttcttctcgc agttgagcaa attgctgata agtctgtgct aaatgctttt gtgtcgaaac
     1981 aaataatgga tagattttct gatatttttt atgatcagtt tcttgtactc caacttgctc
     2041 tttcaattct atcaattcct gtgcttcact gatactttgg atctttccga tgcttctcca
     2101 gcctaaaatc acagctccta atgcggatgc ttctgtgaca gtagggaaaa caagcgggta
     2161 acccagtaca tcagccatca tctgacgaaa aatcggagat tgagcaaacc cgcctgtagc
     2221 acggatctct gtgagctgat tttcctggca aactcctttt aaaatccgat aaaggttgag
     2281 acagatccct tctagaatcg ccttcatcat ttctttttct gtatgttgcc gctgtaaacc
     2341 gataaaactg ccaaacgcat ctgcgtccca aagcggtgct cgctcaccaa gtaaataggg
     2401 atgaaaaatc agaccattgg cacccgctgg cacaaaagcg atagccgaaa gcagtgcatc
     2461 atatggattt tctccttttt ctttagcaga ttgaacgact tcctgtaaaa atgtatgccc
     2521 tgcccaatca aagatgccag caccattgct agtcgctcct cccactaccc aatgtttctg
     2581 atccagaaca taacaaaacg tctccatttc tggatggaac tgaggctgag tagtaataaa
     2641 acgaagtgct ccgctcgttc ctacggtcaa agcggcgatc gtatcaccaa aagcacctaa
     2701 ccctagatta gaaagcggcc cgtctgcccc tcccaaaaca aatgatatat catttggcaa
     2761 acctagttct gctgctgttt ttgatggtag acctgttact tgataggtgg aaggaaagac
     2821 ttgcggcaat tgctctattc gaatgttcaa gtagtcaagc acttcttctg cccattggaa
     2881 ttcatggata tcaaaatatc cagttcctga agctgttccg tagtcgctga tcagttcgcc
     2941 aaacaatcta taaaagatat agtctttgat accgacaaaa taagcagttt ttttgaataa
     3001 ttcaggttgg ctgttttgca gccacttgat tttgttcaat ggtgtcattg gatgaatcgg
     3061 cgtcccagtc tgccgataaa attctttgcc aagcacggta tatttcagtt tttctgcttc
     3121 gtccgctgct cggttatctg cccaagtgat cattcgagat aacggtgttt tctcttcatc
     3181 caacaatatc aggccatgca tcgcagagga gaaggagaca aattgaatga ttgtgtctga
     3241 aggcaactgt ttaattactt cttttataac ggtgcagaca ccttgaaaga tcaattcctg
     3301 atcttgttct gccatccctt gtgtttcttg gatcaaaggg tagcgaaaat aagcggatgc
     3361 tgcttcttgg gcttcatctg tgaaagcaac tgctttggtt tgtgtggttc cgatatctaa
     3421 tccgattgaa acgttcattt tttcacctca aattttatgt atgtacaaaa atttagttta
     3481 agcttgcgta tcttggtttt gaaaaggggg agatggcgtg ttactttatt ttatcattgg
     3541 ttgctgtatc ggatcatttt tatgtttaac cgcccagcgg attccgttag gtcactctat
     3601 tatttatcct agatctcatt gtgtcaaatg ctgtcgttcg ctttcttggt atgaattgat
     3661 tccgattctc tcgatcatcg tccaacgatt tcgctgccgc tattgcaggt gtcggttgcc
     3721 cttctactat ttgttggcag aagccttatg tggtggtctt tttgcctggt tctttacttt
     3781 ttcttccgca agaaattttt cgacgttcat atggatgctt tcggctttgc tattttcgtt
     3841 gatggattta ttttacttca tggtccattc tcacacgcta ttctgttctt gggcaatttt
     3901 atggattttt tggttgcagg ccggcgtctt ccaatggcaa agtgtgctgt taacgtttat
     3961 cgtaggcggt gtgtgccttc gctatggtca atcctatctc ggagctggcg atactctgtt
     4021 gatgctttct tggagtggtg gcttgtctct agaagagttg cttcagatct tgtttcttgc
     4081 tagtctgttt ggaatatccg ttttttctgt ttactttatc tgtcataaaa aaaagttgga
     4141 aaagctgcct ttcattcctt ttttgagcag tgcgttgctg attgtccttc atcttaggta
     4201 ttgaaaaaag gttgtgacaa acgacttgtc acaacctctg atatatagtg ttctcttacc
     4261 aaatccttgt gacttggagc taggcgacag gttcacaacc tctccactaa atggtgttca
     4321 atcagctgac cctcagtatt agctcaaaat gctggtttta caacctctga tatacggtgt
     4381 tccataagta gctttttcgg cttcttattt cttgacgccc caatgtcgac gaaggaaacc
     4441 tgcacgtcca gaaccttctc gataaagatt gtaatggagc ttgtttttct tgtaataatc
     4501 ctgatggtaa tcttctgcag gataaaattc gcttcttggt tcgatttttg tcacgattgg
     4561 cttatcgaat cgcccgctgt cttgcaatgc ttgttttgaa cgctctgcaa tatttcgttc
     4621 ttcttcatca ttatagtaga tcactggacg ataagaatca ccgcgatcag cgaactgtcc
     4681 taatgcgtca gtcggatcgg tttgctgcca atagatttcc accaactgat cataagaaat
     4741 aacttctgga tcatattcga tctctactgc ttctgtatgc cctgttgtgc ctgttgttac
     4801 ttgttcataa gttggatttg gtacatgccc gccagtataa ccggatgtca cagaaatgat
     4861 ccctggctgc tcttcaaatg gtttgaccat acaccaaaag catccgcctg cgaaaactgc
     4921 tttttttgtc atataaaatc cccctttttt ctcatcatac cttgatttcc aaaaggagga
     4981 taacgttttg cttgtgacag aaaaaacgga aactattata tacgtgatac ggcttctgat
     5041 aaatgccaaa acgaacgtac ttgatcaaaa acggttaaat caggaatcac atattcagca
     5101 gaaggaagat ttatgttttc acctattccc aatacatgca tgttaccagc caaagctgct
     5161 tgacaaccag cttctgaatc ttcaatgacc aagcatgcat ttggcggtac attcaattgc
     5221 tgtgctcctt tgagaaaaac ttctggatca ggttttgctt ttgatacttg cgtgccgtca
     5281 ataaggacat caaaataact tgtcaaattc gttttttcta aaatgaggcg tgcatttttg
     5341 cttgctgatc ccaatccgat cttgatgttt gtttttttca agtactcaag aacagctatt
     5401 gcaccaggca aaacggatgt ttcatccatt ttttgaatcg cttgaagata tagattgttt
     5461 ttttgctcag caaaggctga tttctctttt tctgagaatt tctgttccat ttttccaaat
     5521 gacagcaatc gctctagtga ctcggttctg gatattcctt tcagttgctc gttttctttc
     5581 tcagtgaatg gaatggacag ctggttagct aaaaccagcc acgcttcgta atgataacga
     5641 gctgtgtcta caagaacgcc atctaaatcg aatagtgctg ctttgatttt catcgattac
     5701 ctccttgaaa atgaacgact tgaagtaaag atgcgatgtt tttagctgtg cgatgaagat
     5761 tatcagtggc ttgctgtaaa gcttcttgta aaggcacagc tgcaggaagg atcggaaaaa
     5821 cggccgaaat gccaacatct gctgcttttt cgcttccttt accaacactt ccgcaaatag
     5881 ctatcacagg aacgccctct ggtactcttc tagcaatccc aactggcgct tttcctttta
     5941 gactttgtcc atctagacgt ccttcgccta cgattacgag gtctgcttgc tgtacccgtt
     6001 tatcgaactc cagtaaatcc aatacgtagt cgatgccaga gactattttc cctgaagcaa
     6061 atgctaacag tcctgcaccc attccaccac cagcactact gcccgatgcc tcgattactt
     6121 gaggggcaaa tctcatataa aaatcatgca tggcttggtc agccactgct aattcatttt
     6181 ctttcaagcc tttttgtggt ccgaaaacaa aagaagcgcc ttcttttccg cataaaagat
     6241 tagtaacatc agtaatgaca gtgattttgg ctttaatcag ctcaataggc acgttcgttt
     6301 tatcaactgt agcaatttta cctagatttt ctcccagtgc ttctactttt tgattatctt
     6361 gatcgtaaaa agcataacct agaccttctg ccatgccgat gccgccatcg ttggtactac
     6421 ttccgccaac gccgatgatg atgtgtcgaa caccttgttg caacaattgt cgtatcagtt
     6481 ctcctactcc tcgcgtggat aacctaagtg gctgttgttg gtttagtggg acatctttca
     6541 atccgacgat ttctgccatt tcaaaaatcg cagtttgacc gtctgaagcg tactggattt
     6601 ctttgttttt gccaaatgca ttagtaacga ctgctgtttg ccgcttcaac cttaaactgt
     6661 ctgctaatga tgaaaccgtt ccttctcctc catcgcctat aggtagcaga tcatattcag
     6721 cctgaacaaa aacttcttga aaacctgatt gaatggcttg tgctgcctct aaggctgtca
     6781 gactttcttt gaaagaatct ggcgcaatca gaatacgcat tattttcccc tccgttccaa
     6841 gatcagttct ttttccagct ggtattggtc tccattgaca aaaataggta atgtttcttt
     6901 cgtctctttt tttattacta tttgtttttt tgtcaaaaca aatagcaacc tgtgattttt
     6961 ccaaataaaa ggaaaagaaa ttttttccca tgctgaagga agtcttggag aaatagaaag
     7021 agttccattc gttactgtaa tacctgaaaa accaaatata actgctagcc atagtgctcc
     7081 taatgaagca gcatgaattc cgtcatctga tgaatgcgga tctggtccta aatcaatcag
     7141 gcacgcttct tggaataaat gatacgccca ttccatttct tggatacggc aagcctctat
     7201 ggcgtgaatc gctttactca acgatgagtc gtggattgtt ttgttttcat agtaatgcaa
     7261 attcttagaa acgattggtt gagaaaaata ctcaggtaat aagtataaca gcatgacaag
     7321 atctgcttgc ttgctgactt ggagttcatt tacttcgcta cgggcataat caagtaaaat
     7381 agactggctg ccaggacgtt ctttgtaagt ggtcaaatca ataactggct tactgagata
     7441 cgtgtcatct tgcggaatca gctgttcttt gttaggcact ggcaaatata acttatctaa
     7501 aaaattttga cattctttca aaaaaagcgg atcgccagct tgatatttaa cagctaaggc
     7561 cacattataa tgcgccagat agttcgtata tgcattattg tcgatatatt ctgtgtattc
     7621 atctggacca atgactctgt gtatctctaa atgatcatcg acttttacag ctctgctcag
     7681 ccaaaaagct gctgtttcat gaatgagcgt ttttcccttt gtttctaaaa acgattgatc
     7741 ctctgttgct tggacatact cttgaacggc ataagcaagg tctgcactga tatgatgttc
     7801 tgcggcagct gaagcaactt cttgtcttgt tccggttttg atattcaatg cagcaaattt
     7861 gggtgtctct tcctgaccag tgtgtgcgct ttcccatggg aaaagtgcac cttgatagtg
     7921 ataatctctt gctttttgcc gtgcgccttc taatcgatca taacggtaca gcagcaaatt
     7981 ctttgctact tcaggaaact gatataaaaa gaaaggcagg ataaaaattt ccgtatccca
     8041 gaaaacatgc cctttataac cttcgccagt tagtcccttc gcccctacac taacacttgg
     8101 atcattgcgg ggagtcataa tctgtaaatg gtaagcagcg aagtccatcg ctaactgatc
     8161 gaaagaagaa tcgccttgga tcgttatccg atgctgatgc caaaaatctg tccaataaga
     8221 catcgactgc tctaaaattt ctgtataact aacgcttttc tgggcttggg caatactttt
     8281 cttaaacggg tctgtttgct gaagagaggt agagagcacg atgatcttct ccagcgtaaa
     8341 ggacgtattt tttctgatct tttctgaaaa ggaagcggtg atccggcgat tttttgcttg
     8401 gaatacacct gctttattga atttagctgc aatcccaata tcaattttac tttctgttgt
     8461 ctggtaggat gcactgatca aatcgtcacc aaatactcgc agttcttttt caaccaactg
     8521 ctgtgtacca aaattggttt gctgtccatc gatacccgtt ttcacatcta cttggcagtc
     8581 ttcggtcaac ggcttgatga ccactttcag ggctgcctga tggagattat tttgatcagc
     8641 aacacgacaa gtaatgactt ccactttttt tccttgagca gtttcaatga gcgcatagcg
     8701 tttaagctct cccatttcta aatctagtgc ccgctcgtat gcaagaactt tcatcgtctc
     8761 aagagaaaaa tgttcaccgt cgatttttat ttcccatctg atgacatctg ggagattcac
     8821 taactcggct gattctgctc ctaaaggtcg gttatataca ccagctgcaa acattcccct
     8881 tacttgattc cgatagtctt cctcctggct tcctctgata ccaaaaaatc catttccaat
     8941 ggtcatctga ctcgcatact tattgatata ctctaaatca aactgttttt cttctacttt
     9001 cataaggtaa taacttctcc tgtttctgcc gaacgatata aattttccac cagattctgg
     9061 atgacatatc cttgccagcc atctgcaatc atctgagtct ggtcaccgag cgcccgattc
     9121 acaaaatgag ccatacttag ttcatgtggg tcattaactg gcgtggtctt ttgtaaggaa
     9181 accagctttc catctacatc ttcgaatagt tcgcctggat acagattagc acctgcctga
     9241 tcgccacaaa aagccacatt cagtttgtct tctctgatat tcaaagcaaa cgaagtattc
     9301 agttggatga tcttgccgtt ttctaattcc agtgtgccaa aaattgcatc ttctactgtg
     9361 aatttgcttg gatcccattt cccgaatttc cctgaatttt ttttcgtacc aattttttgg
     9421 aagctgtacg cccgaacgct ttttacttta ggaaatccag taatatacat cgctgtatca
     9481 agcatgtgaa tgccataatc gatcaaaggc ccgcctcctt gaagttcttt gtcgataaac
     9541 gttccccagc ctggaacacc agaccgacga agcgcattcg cttctacgta ataaatatcc
     9601 ccgagaacat caagatgatt cttgatcatt gttgcttcgt cagaaaaacg atgctggaat
     9661 ccataagcca aaatctgccc gtttttatcg gctgtctctg ccatctttct tgcttgatca
     9721 gctgtcatag ctggtggttt ctcgcacatc actgaagcat tagcttcgag ggcagccaat
     9781 acatgttcga agtgaaagcg attgggtgta cagacactca cgatatcggg agaaatctca
     9841 ttcagcattt gtgtaactga tgtatacgca tgagaaaatc ctgtacgtcg gcaaaagtct
     9901 tctgctcgct ggctgactgg atcaaccaca gcggccaatt ctaagacatc tgatcttttt
     9961 tgataataag cagcatgtac tttttctgct acttgtccag cgcctatgat tgctactttc
    10021 ttcatttatt tctctctcct ttgcttattt acttattgtt attctttctc tattctttct
    10081 ctaaatcatt taaatgacgc gctgtttcca gacagttttt caagtattgc aatgattcct
    10141 gataaatttt cacttcattt tctcctcgca aacgacattc atataccata taaccttcat
    10201 actcattttg aagtagcgtt tgaaaatggc gagtaaaatc aattgcgcca gatcctggtt
    10261 ggtagcgatg attatctgct aaatggatat gacccacata ctctttttgc gtagtcaatg
    10321 cttttgaaat atcatcttct tcaatattca tatgataaaa atcagcgatg atctgcacat
    10381 gtttcaattg attagagtca atgtaatgac gcgcatccgc taacaaattg atcatatggt
    10441 cttggtatcg gttcaaaggc tccaaataaa taaccgtacc tgtttcttcg gctactttat
    10501 ctaaataacg aagtgattcg ctgactgctt tatagtctcc ttctttgctt cggggtgaaa
    10561 ccatcggcgg taatcgataa gtgaacattc cccaagctgc tgggataacg attcctttgc
    10621 ctccaacctc gcttagtgca cgcaaaatct cagcaatttg acgtacacca ttaagacgcc
    10681 gttcttcgat aaagtcaccg atccacccat cgtagccgtt acaagctgtc actactggga
    10741 tccctgtttc tttgattgcc tgtttgactg tgtcgatatt ttctattaac aacttaccgt
    10801 cgatttcgta ttcttcaaat ccacagtctt tgataaatct aaatttttct aaaatgtctc
    10861 ttgggaaaaa tgcttgatct tgcgtaccga atttcattgt tctcctcctc ggacttgaac
    10921 gcccattttg atactttctt ctgggtgctg gtctacataa cgcatgtaag cttctgcgct
    10981 ttctgcaaat ggcacaactg gatcaatgat ttcttcacaa tccaagtaac cgttcatcaa
    11041 taattcccaa catgtgtcct cgattcgttt acggttccat cttgggtaat ctgggtttgg
    11101 ttcactagat gctcgagcaa agacgatttt tgcattattg aaatgtgctt cgcgaccaag
    11161 attaaaccca gctggaaaag gtttagcaaa ggcaacataa gaaatgatcc cgccatagcc
    11221 gatccctatt aaagaagctt gtaatgcggc agcactgcca ctcgtttcaa taactgaatc
    11281 tactccacgt ttatctgtca attcctttag acgaaaaccg acatctgctt tcataggatc
    11341 tagcgtgtaa tcggcaccgt gtttttctgc taattctcta cgctgcgcta tgggatcgat
    11401 cccaacgatg gtagtagcac ctgctttttt agctaattga atcgcaattt gcccgatagc
    11461 gcctaaaccg aagattgcta cagcatcacc tgctcgaaca tgtgcttccc ggataccacc
    11521 taaagcgaat tgagcaggat cgtaacatac tgcatttttc cattgatcca accgatcaag
    11581 tttacgtagt ttataattat caacaccgtt tgtaatcagc gtttcacgaa tgggtccata
    11641 agtgcagact aactctcctt tttgatactc tgtcaccaaa ctgcccgttt cttcgatcgt
    11701 accgacaatc atatttccta attgaaattc gccaaatacc acaccgcgtt ggcttccttc
    11761 ttctcttggc aagaataggc gccattcttc tgaaaattct tcatcgataa atggacttaa
    11821 tcctctaaag tcgacaattt ctgttcctgg ttttggagta gcgaaaatta ctcggatacg
    11881 tacttcgttc ttttcgatct cacggtcttc gtaatctttg aatccggcta ttcttggttc
    11941 taatgcaact aattttttca tcttttcttt cctctttctt ttctttttat ccttttacgc
    12001 cgccttcgac taaatcacct ttaataaatc gttcagataa cgcatacata atgatgactg
    12061 gtaaagcggt caccaatgaa gcggccatca ttcgtcccca aatgtagtct ggtgtactga
    12121 ataatgtgtt caaaccgatt ggtaaagtaa atttttcgga ggtcgacagg aaaatcgaag
    12181 caaataaata atcattccac gaaaccataa agcaataaac gaagacagaa atgattccag
    12241 aaatagccaa tggtataatg atccgaataa tgatctggat ccggctaaga ccatcgatca
    12301 ttgctgcttc ttcaatatca gtcggaatcg tgtcaaaata actttttagc atgaatatcg
    12361 cagtaggaag tgtttgaacg accatcgtga taatcagtgc tgtattggta tcatacaaac
    12421 ctagaccgga aatgatttta aataatggaa caacgatcaa aataccggaa aacatataaa
    12481 cggtgtaaaa gctagcattg attttcgagc gagctttgaa gcgtagtttt gacaaagcgt
    12541 atgccccgct gatcccaatc actactgcag ttacggaggc tatcaaggag atcactaaac
    12601 tatttttgaa atacatgatg aatggaaaaa tctcagcatt gaaaatatcc tgataatgtt
    12661 caaaagtcca ttctttcgga aaaatagttt gtgtcgttgc aattgcttct tgtgaatcct
    12721 tgaatgatgt catcagcatg atcaaaaatg gaaacaaatt gacagctaat agcaacaaaa
    12781 tcgtcgcgta aaataagatt ttcctgctct ttttattttt catttcaagt ccaccttctt
    12841 tcgtgagagc aagacgaccg ctgcaataat gatgaacaat agaatcgata tagctgctgc
    12901 ttttccaaga tcattgaacg caaaggctgt ctgatacaaa taaacgccta agatattgac
    12961 tttattcgtc agtagataga catctgtaaa catatagaac atccagatag atcgtaaaac
    13021 aatcacagtt gctagtactg gtttgattgc cggcagtgtc acgatcttga acttctgcca
    13081 agcatttgca ccgtccattt cagctgcttc ataaagcgtt ttgtcgactg tttgcaaaat
    13141 agccaaaaat gaaataaacg catacggata gtatctccag atagcaaaca aaacgaccat
    13201 aaagaagcta gcaactggct ggtcgaacca cagcggtgct tcgttgaaaa gctgcaacct
    13261 atctactaac agatcattga cgattccata actattattg aacatatatt tccaagcaaa
    13321 aatcaaagaa atagatggcg taacataact taataaaatc agtgaacgca ccgttttcct
    13381 aaatctgaac ttgcggttaa gcaaaatagc tacggctaat cctaaagctg tactgcctgc
    13441 tacaactaag aatgtgtaag caactgttaa acctaaagaa cgataaaatt cactatcact
    13501 taaaatttca agataattgc ttaatccgat aaaaacggcg tctgagttag gattcagcgg
    13561 catatcgaaa aaactgatct gaatattgga aagcattgga atcagcacta aaacagctaa
    13621 taaaagcatg cttggtgcta aaagtcccat ggctaatttt aagtcagaat gtttccttgg
    13681 acgattcatt gaaacctccc cctgataatc tgccaatagc tatctacttt tttcgaacac
    13741 cattggcaga acttcttgtt atttcgtatt tttttgtgcg tcagctaaag cttgatcgac
    13801 atcttcgttt ccgacagtta cttggttcac tgcttttccg atcacacctg agcttgtgat
    13861 atctcccatt ttcgtgaaat tcttttcccc gactagtcca aagacttgta cttcatcaaa
    13921 agcactagca atctcagatg gcagctcgcc aaaagacttg atgacttcat tttctttgta
    13981 accatccagt tcgactactt gtttattcac tggttgagca ccaccaggag acatcaagac
    14041 ccacttttcc atgtttttcg gttgagataa gaaagctgtg aatgcttcag cagcctcttt
    14101 ttgtttctcg tctagaccag aagaaatcgt caatccagat actgtgccat agacagcttg
    14161 atctttttgt gttgggatag caaaaccgat attttctgag ttccccgctt cataaacaga
    14221 aggcaaaatg taagttgaat agattgccat tggtgctgtt ccattcatga aagcatcttt
    14281 gatttctgtc acatcattag agcctggcat cgtgtattgg gataagtttt gataaaacga
    14341 caatgcttct ctcatctttt ccgtatcgat cgttacctcg ccttcatcat ccaaaacatt
    14401 tgctccattt gacaaagcaa attgagagaa cgcttgctca ctcattgttc cttcagcagt
    14461 aggcatagca attccatatt gtttattctc tttatctgtg aaatgttgag caatttttaa
    14521 aatatcatcc caattttctg gttctgaaaa accagcttct gataattttt ctttgtcata
    14581 ccaaatacct tgaacccagc cgctaattgg tgcagcaata tagctttttc catcttctga
    14641 acgaacaagg tttttggctc cgtcataata attatcttct ccagcttcct cgataacgtt
    14701 ttggactgcc gtctgatcaa tcagttcatc tttatccatc actttagcaa aatcctgact
    14761 gacttccatt acttctggta atttaccgga acgagctaac gtaacgattt ttgtattgaa
    14821 cgctgattct tctaccggca cttgcttgat tttgatgtct ggattttctt tttcaaaatc
    14881 agcaaccagt tcattgatca cacttaatcg ttcctgttcg acagaagagt gcatgaattg
    14941 aatcgttgtc tgttgtgatt cgttagagct ttgactttca ccacaagcag taagaagaaa
    15001 tccagtagct agcagacagg tagtaacaat aatttttttc atttttttcc acctcttatt
    15061 ttattatttt cagccacata tattcatacg gctggaatat caactttcgt tcttgactgc
    15121 aatggataat ttgtccagaa aacaggtcta ggtaatctcc ttttggtaaa agataacctg
    15181 ctgattgatt ggaaaaatta tggataacta agactttctc ttccttcgct tcgcgaataa
    15241 aactgataaa agaatctgct gagtccagta agtaaatcaa actattcgga tggaaggctt
    15301 tttctttttt tcgaatagat agcatctggg tcaattcacc atatactgct gttcttaatg
    15361 gatctgtctg caactctttc tcaatatctg ctaacaaaaa tttttgccga ttgatttttc
    15421 gattctctcc tgttttttct acgcctttgt aatcatttcg acttcccagc acactttgaa
    15481 tataaattgc cggcacgcct ggtaacgtta ccaaaaatga atgagcagct aaaaagcgtt
    15541 tgcttctcca ttccgtcgta tctgttggag aacttaacgc atctagatac gtagcgttta
    15601 tctcatatgg actggtcgtt ccatctgggt ttttcttgta gtttactcgg gcaccctctt
    15661 tcttcagctg ttccactaat tcaaggatcg ttttttccgg cagaatacct cgtaccggat
    15721 tcaatccgat accgtcatgg gaggctaaaa aattaaaaaa agttgttttt ttcgatgcga
    15781 aatcgatggt ttttaaccat tctttcaact tacttgtatt acccgaacga atcgcttgaa
    15841 gggtcaaagg tggtagcgaa aactgataga ccatctgtgc ttcattctcg ccattcccaa
    15901 aataactgat attatctttg tgtggcacat ttgtctcagt gatcaatacc gttcctggag
    15961 ctgcttcatc agcgattgca cggaataatt ggatgatttt atgcgtttct ggcaggtgta
    16021 tacaagaagt tcctagttct ttccataaaa atcccacagc gtccaagcga ataaatgaag
    16081 cgcctttttc taagtaaaac aaaagaatat cgatcatttt caataaaact tctggatttt
    16141 taaaattcaa gtctacttga tcttcgctaa aagtcgtcca tacgtgtttt tctttcccgt
    16201 ttttgtcaat gaacgttgta agtaacgggc tggtccttgg tctagtcacg ctgcttgtat
    16261 ctgtatccag tggcaaatca ataaaaaaat ctttgtacgt atcattcaag tttaaatatt
    16321 cctgaaacca agtactttcg gcagacatat gattgcagac aaagtcgaac attaaccgtg
    16381 tttcgcttct catttcttct atatcttccc atgtgcccaa acggtcgttg attgcctgat
    16441 aatttacaac ggagaaacca tcatcagatg tatatgggaa aaatggcaag aaatgtacaa
    16501 ctctaaatgt atctgccaaa tacttttgat aaaatttctt aaatgtacgc aaagttggtt
    16561 gctgctcttc aaaaaattga tccccataag taatcaagaa aatatcttct tcgtcccaat
    16621 gcagtttgcc tgcctggcct ttcttctgct gataaacact gactttttgt tcaatcatag
    16681 aggcggtttt ctcagacaaa tctgagtcgc gataaatcgc ttgtaaaagt tttgtataca
    16741 tcggattccc ctttcttttt tgggagcgtt cccaacgatt tgatcatatt atggaaaacg
    16801 cttactgtca atagcatttt tttaaataat tttgatatac acaaaaatac aaataagaaa
    16861 gcttatgtat caataattaa tagcttctta aaaaaatatg gtctctcatg atcatgcaag
    16921 ctattcctaa attttcaaaa tcatagacaa aaaagaatgt ctattgtact atggaattat
    16981 tctaaaacgc tcccaaaaag gaggtgcata taaaattgaa acctactatt tatgacattg
    17041 cgaaaaaagc taatgtctca aaatcaactg tctccagagt attaaataac cagccaaata
    17101 tctctgataa agcaaaagca agtgtacttc aagcaatcaa agaattgaat tatgaaccga
    17161 gcaaaattgc aagaggactg tcaggaaatg gttttgacgc gattctagca ttatctcatc
    17221 gtacttctca tacgacctat ggcaatcctt ttttttcaga tgttattcaa tccatttctg
    17281 ccgtttctga acaaaataac ttcgacttga ttctacaaac ggcaaaaaca ccagaagaag
    17341 agctagaaaa atgcgcgaaa aagatccaag aaaaaatgat ccgaggaatt attattttaa
    17401 gctctacgat ggatgaaaca cttctttttg aattggatca atacaatatt cctattgtgg
    17461 taatcggaaa agtcggaaac gactacacga acatctactc agtggacacg gataatttcc
    17521 aggatagtta cgatttggtg gaaacactga tcaagaatgg acaccgtcat atcggctgtc
    17581 tccatgctcc gacagaagtt tatgtatcca atgatcgtct caatggttac cgtcaatgct
    17641 tatttgatca ccacctagat atccgtaatg aatggattat agacggcggc tttcgcttag
    17701 aagatagcat ggcagcaatg aatgatttaa tgaagctacg agaaagacca actgcgattt
    17761 tttctaccga tgcattaaaa tcattgagta tttacaaagt ggcagaacaa tctggaaagc
    17821 agatacctga tgatctttcg atcgcaggat tcagtgatcc cttattgtcc tccctatttt
    17881 cgccacagtt gactcggata gacattccga caaatcagtt aggaatcaaa gccacagaac
    17941 ttttattcca actaatccac caccaatacc ctgaagaaaa aagaatcatt ctttcaaccg
    18001 agattactct cacagattcg atccaacaac aagaaatgta gaacagaatt ataaaagaac
    18061 agaaacactg aggataagtc agttactctg ttcttttttc aatcactcat ccctcgttac
    18121 cccctcccgg tattttagcg aatttttctt attttccatc tgttttagat agctgtggat
    18181 aactataatg tttgtgttaa actatttttt ccacagggaa gaaaaaacta tttttaacca
    18241 cgttgtgtat aagtttttat agttcgtttt tttaaacaca tttaccctta tttcaatcca
    18301 tgttatttgg ggatacttat ctctaaaaca taaatagaat aacagacatg tctactggtt
    18361 ttgttttatt cgaggaatta ttcaataata gctagcaaaa tattaaaaat aaccgctcta
    18421 tcttcccatc aatttatttt gcattcaaaa aatgatctat ctttactttt gttgattgac
    18481 ttctcttgga agatgatcgt tccgatacct taattggata cgaaccgatg ttgttaagat
    18541 cctttcccac ttttcttcta gttagacaaa ttattttaga caaaccaaaa aagaggttgt
    18601 gacaataatt tgtcacaacc tcttttttaa aatatctatt ttgtttgatt gttcattgcg
    18661 tcttctgcag ccatttgcgc ttcaatatct gaaatactgt agacattttc gcttgctaca
    18721 ccgacttctt ttggttccat gtcacgataa cgttgcatac cagtaccagc tgggatgatc
    18781 ttacctataa taacattttc tttcaaacca agaagaggat ctttcttacc gcggatcgca
    18841 gcatccgtca agacacgagt tgtttcttgg aatgatgcag cagataagaa actatttgtt
    18901 tctaatgatg cttttgtgat acctagtaag acaggtcgag aagttgcagg aacgcctcct
    18961 gagactagcg tattgtagtt ttgatcttta aagtctgcga tgtctaataa tgtacctggt
    19021 aagatatctg tatcacctgg atccattaca cgaactttac gcagcatttg acgaaccatt
    19081 acttcgatat gcttgtcgcc gatttctacc ccttgcatac ggtaaacacg ttgtacttca
    19141 cgaagcaagt agttttcaac agataataca tcgcgaactt gcaacaattg ttttggatcg
    19201 attgatcctt ctgtcaatgg tgcaccacgg tggatcatat cgccttctgc tactttcata
    19261 cgtgctgtat aaggaacagt atacgtacgt gtatctgttt ttcctttgat tgttacttct
    19321 ttggtacgtg ttgcaggatc ttctgagata tcgatgacat cccctgttac ttctgtgatc
    19381 actgcttgcc ctttcggatt acgtgcttca aagatttctt ggacacgagg aagaccttga
    19441 gtgatatcgt ctccggcaac cccacctgta tggaatgtac gcatcgtcaa ctgagtacca
    19501 ggttcaccga ttgattgagc ggcgatcgta ccaactgctt ctccgacttc aacgtcagag
    19561 ccagttgcaa ggttgcgtcc gtaacaatgt ttgcagacac catgttttgt attacatgtg
    19621 aagacagaac ggattgtcac tgtttcgaca cctgcatcaa cgatttgttt tgcgatgtct
    19681 tcggaaatca attggtttcc tgcaatgatc aattcatttg tttcaggatg acgtacttct
    19741 ttacgggtgt aacgtcccac taaacgatct tctaatgatt cgatgatttc atttccctcg
    19801 cggatggctt ggatttcaag acctcggtca gtaccgcaat cttcttcacg gatgattaca
    19861 tcttgggcca cgtcaaccag acgacgagtc aagtaacctg aatcggcagt cttcaaggct
    19921 gtatcggtca tccctttacg agccccgtga gttgagataa acatttccaa gacggatagt
    19981 ccttcacgga agttcgaaat gattggcaat tccatgattc gaccattagg agcggccatc
    20041 aaaccacgca ttccggcaag ctgtgtaaag ttggaaatgt taccacgagc tccagaatca
    20101 gacatcatga agattggatt tttcgcttcc aaaccttcca tcagtttttg ttggatgctg
    20161 tcttttgcag cattccaaac agcaatgaca cgttcataac gttcgtcatc tgtgatcaga
    20221 ccacgacgga attgttttgt aatagtttca acttgtttat gggcattttc aatgatttct
    20281 tgtttttcat tcagtacact gatgtcagcg atccctactg tgataccggc atgtgtcgaa
    20341 tgtttgtacc ctaagtcttt catacggtca agcatcttag aagtttctgt tactttgaat
    20401 cgtttgaaga cttctgcgat gatgtttcct aagtttttct tcttgaacgg taatactaat
    20461 tcttgctctt tgatatgtgc aggaatatca gtaccagctt caacaaagta tttgtccggt
    20521 gtttggacgg tcaagttgaa gtctgttggt tcattcagat aagggaattc aggaggcatg
    20581 atctcgttga agatgatctt accaactgtt gtgatcatcg tacgttcttt ttgccattca
    20641 gtaaatggtt tttcacctaa tgagttaggg ttcacaccga tacgtgaatg taaatgaaca
    20701 tatccgttgc gccatgcgat aacggcttcg ttttgatctc tgaagaccat tccttcgcct
    20761 tcgcgtcctt cttcttccat tgtcaagtag tagttaccta gaaccatatc ttgagaagga
    20821 gtaacaactg gtttaccatc tttagggttc aagatgtttt gagcagctaa catcaacata
    20881 cgtgcttctg cttgtgcttc ttcattcaat ggaacgtgga cagccatttg gtctccatcg
    20941 aaatcggcat tataagcttc acatacaagt ggatgcaaac ggattgcgcg tccttgtact
    21001 aaaactggtt cgaatgcttg gataccaagt cggtgaagcg taggtgcccg gttcagcaat
    21061 actgggtgtt ccttgattac gtcttctaaa acatcccata cttcgtcttc ttgacgttcg
    21121 attttacgtt tcgcattttt gatgtttgaa gctagttcgc gttgaacaag ctcacgcatc
    21181 acgaatggtt tgaataattc aatggccatt tcttttggaa gtccacattg gtacatcttc
    21241 aagaaaggtc caacaacgat aacggaacga cctgagtagt cgacacgttt acctagcaag
    21301 ttttgacgga aacgaccttg tttccctttc aacatatgag aaagagattt caatgggcgg
    21361 ttacctggtc cagtaactgg acgtccgcga cgaccgttat cgatcaatgc atctactgct
    21421 tcttgcagca tacgtttttc attttgaacg atgattccag gtgcattcaa gtctaacaaa
    21481 cgtttcaaac ggttattacg gttgattaca cgacggtaaa gatcattcaa gtcacttgtt
    21541 gcaaaacgtc caccttctaa ttgaaccatt gggcgtaaat ctggtggaat aacagggata
    21601 acgtccataa ccatccagct tggttgattt cctgaagcgc ggaatgcttc taaaatatcc
    21661 aaacggcgga ttgcacgtgt acgtttttgt ccttgtgcag atttcaattc ttctttcaat
    21721 tcggctgctt ctgcatctag atcaacatta tcaagtaatt gtttaatcgc ttctgcaccc
    21781 atagcagctt ggaattcttg tccatattgt tcacgttttt cgcgatattc gcgttctgtc
    21841 aacaattgtt ttttctctaa attcgtattt cctggttcga tgactacata tgaagcaaaa
    21901 taaatgattt cttccaaagc gcgtgggctc atgtctaata caagacccat acgtgaagga
    21961 atccctttaa agtaccagat atgtgaaaca ggagctgcta attcaatatg tcccatgcgt
    22021 tcacggcgaa ctttcgaacg agtaacttcc actccgcatc gatcacaaac gattccttta
    22081 taacggatac gtttgtattt tccgcaggca cattcccagt cttttgtagg accaaaaatc
    22141 cgctcacaga acaacccttc gcgttcaggt tttaaggtac gatagttaat cgtctcaggt
    22201 ttttttactt caccgtaaga ccagcttctg attttttcgg gagaagccaa acctatttgc
    22261 atactttcga atttatttac atcgatcaaa aggggttccc tccatttcat ttaatggcaa
    22321 accgcaagac ggctgatctt cgaccgcctt gcagctcata gaccggatta gtctaaatta
    22381 tcttctgcgg tttcaaagtt ttggatgaca tcgtcttttt catcttccac ttgttcagca
    22441 gctttctttt ctaattcttt ggcagtttgc tgttcggcaa attttgtcaa agcatcaaca
    22501 gtgatcaaat cgtcgtcttc atcgtccatg tcgcgaagtt cgatctctgt ttcttcgata
    22561 tccaagacac gcatgtccag acctagtgat tgcaattctt tcaccaatac gcggaatgat
    22621 tccggtacac ctggttttgg aattggttca cccttgacga ttgcttcgta tgttttcaca
    22681 cgtccgacta cgtcatctga tttgtatgtc aagatttctt gtaaagtata agccgcaccg
    22741 taagcttcca gtgcccatac ttccatttca ccaaaacgtt gtccaccaaa ttgagcttta
    22801 cctcccaatg gttgttgtgt aaccaatgag taaggtccga ttgaacgtgc atgcaatttg
    22861 tcatcgacca tgtgagcaag tttgatcatg tacatcacac cgacagacac acgtccatca
    22921 aatggttcac ctgtacgtcc gtcgtacaga acagttttcg catcgctggc cataccggct
    22981 tcacgaactg tttcccatac gtcttcatca cttgcaccat caaatactgg cgttgctacg
    23041 tggataccta actgacgtgc agccataccc aagtgtaatt ccaatacttg cccgatgttc
    23101 atacgtgaag gcacccctaa aggattcagc atgatatcga ttggtgttcc atcaggtagg
    23161 aatggcatat cttcttccgg cataatacgg gatacaaccc ctttatttcc gtgacgaccg
    23221 gccattttat ccccttcatg gattttacgt ttttgaacga tataaacacg aactaacatg
    23281 tttacgcctg gtgacaattc atctccagct tcacgagtaa agattttcac atcgtgaacg
    23341 ataccgccgc cgccatgtgg tacacgtaat gatgtatcgc ggacttcacg agctttttcg
    23401 ccaaagatcg catgcaatag acgttcttct gcagataatt ctgttacccc ttttggcgtg
    23461 actttcccta caagcaaatc gccatcttgg acttctgcac caatacggat gatacccatt
    23521 tcgtctaaat cttttaatgc gtcttccccg acattaggga tttcgcgtgt gatttcttca
    23581 ggtcctaatt ttgtatcgcg ggcttctgat tcatattctt caatatggat agaagtgtat
    23641 acgtcatctt ttaccaaacg acggctcatg atgatcgcat cttcatagtt gtacccttcc
    23701 caagtcatga aaccaactaa aacgttttgg cctagagcca tttcgccttg ttccatagat
    23761 ggtccgtctg ccagagtatc ccctttttcg actttctcac ccaaatgaac gattggacgt
    23821 tggttgtagc ttgttccgga gtttgaacga cggaattttg ttacatcgta tttatctaat
    23881 gcaccgttgt cgcggcgtac gcggatttca gaagcatcaa cgtattcaac gacaccgtca
    23941 tgtttacata acaaagcagc tcctgagtca tgcgctgatt tgtattccat accagtacca
    24001 acccatggtg cttgaggatt gatcaacggc actgcttgac gctgcatgtt ggcacccatc
    24061 aaggcacggt tggagtcatc gttttccaag aaaggaatac atgctgtcgc aacggcaact
    24121 acttgtttag gtgatacgtc catgtagtct actttatcaa tagatacttc taagttttca
    24181 ctttgagcac gagccataac aacgtcttga gcaaatgttc catcttcatt caatgggctg
    24241 tttgcttggg ctacgatgta atggtcttcg atatcagctg tcaagtagtc gatttggtca
    24301 gtaacacgac ctgtttcacg gtctacacga cgataaggtg tttcgataaa tccgaattta
    24361 tttactttcg cataactgga caaactattg atcaacccga tgtttgggcc ttcaggagtt
    24421 tcgatcggac acatacggcc atagtgggag tagtgaacgt cacgaacttc ataaccggcg
    24481 cggtcacgag tcaatccgcc aggtcctaag gctgaaagac gacgtttgtg tgtcaattcg
    24541 ccaagagggt ttgtttgatc catgaactgt gacaactgag aagaaccaaa gaattctttg
    24601 atacttgcca caactggacg gatattgatc aattgttgag gtgtcaatgt ttcagtatct
    24661 tggatcgaca ttctttcacg aaccacacgt tccatacgtg ctagcccgat acggaattgg
    24721 ttttgtaaaa gttcaccgac tgaacggata cgacgatttc ctaagtggtc gatatcatcg
    24781 acattgccga taccttccat caagttcaag aaatagctca ttgaagcaat gatgtcggct
    24841 ggacgaaccg tttttacagc tgcttctgga tagccgtttc cgatcacgtt tacttcacgt
    24901 tcagggtctc ttggtgaaaa tactttgatg acttggacag tcattggatc tgtgaccaca
    24961 ccgtcttctg atggatagta agtcactgaa tttaggccat tatcaatgaa aggagctagt
    25021 gtttccatga cttggtgagt cagaactgtt cctttttcaa cgatgatctc gcctgtttct
    25081 ggatcaacta atgtttcagc taatgtcaga ttcaatagac gtgttttcaa atctaatttt
    25141 ttgtttactt tgtaacgacc aacgtttgct aagtcgtaac gttttggatc gaagaaacga
    25201 gcattcagca agttacgtga gctgtctgct gtttttggtt cgcctggacg caaacgttcg
    25261 taaacgtctt tcaaaccttc ttctgttctt gagtcgcttg cgtttttatg caagtctttt
    25321 tcgactgtat tgcgtaatgt ttcgctgtcg ccgaagattt caaagatcgt atcatcggaa
    25381 ccaaatccta acgcacgaac taatacggtt aaaggaatct tacgtgtacg gtcgatacgt
    25441 acataagaga tgtcttttgc atcagtttcc atttctagcc aagctccacg gttagggata
    25501 acagtagaac cgaatccttc ttttccgttt ttgtcgactt ttccatggaa atagacacct
    25561 ggtgaacgga ctaactgaga aacgattact cgctctgccc cgttgatgat gaatgttcct
    25621 tgttctgtca ttaatgggaa atcaccgaag aatacttctt gtgctttgat ttcgccagtc
    25681 tcgcggtttg tcaaacgcaa tgttacatgt aatggtgcag agtagtttgc atcatgcgca
    25741 cgagcttctg ctactgtata ctttggttct ttcaattcat aatctacaaa ttccaaagat
    25801 agattgccat tgaagtcatc gattggcaaa atgtcttcaa acatttctct aagtccctca
    25861 tctaagaacc attggtaaga gtctgtttga atttcgatca aatttggtaa ttccaacact
    25921 tcactgattc ttgcgaaact tctacgttcg cgatgttttc cgtattttac tacgtgtcca
    25981 gccaagctct tcacccctct aaaatgtcat tcaaaaagac tacccgatca attgttacaa
    26041 taatatttcg aatattattt ttaggtaaca acagacattt tttgcggctt ttaggcaaaa
    26101 aaaaaccaaa aatagaacat tctcttgaaa ccttacttcg tgagcttcta cttttgtttc
    26161 cttatattaa agccgaaacg gacaatattt cgtttctgcc ttttcttcat cagatagttt
    26221 ttgcaacttc atatactaca cttattttct ataaaagtca acacttcctg gcgtttcttc
    26281 tgctttttcg caacattttc ttaatgtttt caattttatg ttttcaatta tctgacaatt
    26341 cttaattgac agaccttttt atctcggtta tactttagaa aattcatttt gtggaggaac
    26401 ttctatgcga accaacatca accaaatcgc accaacacaa ctgaataact tttactttag
    26461 ctactttaga taggtttgta ttcatcatga tggatacaaa cgttaaacga cagtttgtat
    26521 ctatgatggc taaagaactt tgtattgcag caaaaagcca cgtagacaaa atgctaagtg
    26581 gctgagcagt gaaaatacaa agtaaaagta tcaagcattc tactttttca atttattttc
    26641 cgacttttca atccatttgg acattttgtc tgaatggatt ttttgtttcc caaaattaag
    26701 gaggaaaaag aaatgaaaaa aaatataatc atcggattag gcgctgccct gctactagct
    26761 ttaggaggtt gcgcacaaaa agacaattcc gcagaggcat cagatggaca aaaaacaatt
    26821 ggcgtattgc aacttgtaca acataactcg ttagatgctg cctataaagg attcaaagaa
    26881 ggattagcag aagaagggta taaagaaggc gataatctaa caatcgatta tcaaaatgcc
    26941 caaaacaatc aagacaacct gaaaagtatg agtgagaaac tcgtcaaaga agatcctgat
    27001 ttgctacttg gaatcgccac tccagcagca caatctttat taaatgaaac aaccactatc
    27061 ccgatgactg tcacagcggt cacagacttg gaacaagccc atttagtcac atccaatgaa
    27121 aaacctggcg gcaatgtgac tggcacgaca gatatggtgc caatcgaaaa acaaatcggc
    27181 ttactgttaa atatcgtgcc agacgccaaa acaatcggca tcatgtataa caacggtgaa
    27241 gcaaactcta agatccaagc tgatttggca gaaaaagaat tgaaaaaagc gggcgtcaaa
    27301 gtcaaagtac tgacagcaaa ttccacaaat gatgtccaac aagtgacgaa aagtctagct
    27361 aaagatgtag atggaatcta tattcctact gataacacgt tcgcaagtgc ggcaagtgtt
    27421 gtcggtgaag tcgcaaaaga aacaaaaaca cctgtcgttg ctggcgcagt tgatcaagtc
    27481 aaagacggcg gtctagcaac agtaggaatc gactacgaaa gcttgggcaa acaaacaggg
    27541 atcatggcag caaaaatctt agacggcaag aatccaagtg aacttcctgt cgaaacagcg
    27601 aatgatttgg aactgtatgt caataaagac atggccaaag cattaggcat caatccagac
    27661 agcatcaaag aagctgaata aaacacagga ggattctcat gacaaatttg ctctctcttt
    27721 tcttatcatc tgcatcgcaa ggggtcttat gggccctact tgcgattggt gtatacgtca
    27781 ctttccgtat cttggacatt gccgacttga ctgctgaagg aagctttcct ttaggtgcag
    27841 ggatcgcggc agtaagtatt acaaatggct atcatccatt gacagcttgt cttctaggct
    27901 ttttaggggg tgcggcagca gggcttgttt ccggactgct tcacacaaaa ttgaaaattc
    27961 ccgcccttct tgcagggatc atcaccatga ctggtttata ttctgtcaca tcccgtgtga
    28021 tgggtgcgcc gaacatctct ttattgggtc agccaacgat ctttcattgg gtcgagtcat
    28081 tcggcataac aaaagacaac gctgtactga tcgtcggtct gctttttgct ttgatcgttg
    28141 tatgtttact tgttttgttc ttacgtacag aaacaggact tgctattcgt gcaactggcg
    28201 ataatctggc aatgagcgaa gcaaacggga tcaacacaga taatatgaag atcatcggtt
    28261 atatgatttc taatggatgc attgctcttt ctggctcctt acttgcgcaa aacaacggct
    28321 ttgctgattt aaattctggt attggaacga tcgtaattgg acttgcttct attatcatcg
    28381 cagaagtttt gttacgtaac cagccactat ttctccgtct cttgactgtc gttttaggtg
    28441 cggtcattta tcgttttatt ttagcgttag tcttcgaact gaatgttgaa ccaagtgatt
    28501 cgaaacttgc ttctgctttc gtgctagtga tctgtctttc tttcccgcag atccagcaaa
    28561 aattccattt acctcaaaaa aagaaaggcg gtagtgtcaa atgaatgacg tcttaacatt
    28621 ggaaaatctc catcatacct ttgaaaaagg aacgatcaac gaaaatcatg tcctgaaagg
    28681 cattaatctt tccttagatc ccggcgactt cgtgacgatc atcggtggaa atggggctgg
    28741 taaatctact ttgctgaaca gcatcgccgg tacattttct gtcgatcagg gtcaaatttt
    28801 attgaatgga aaaaatatca cgaaaaaatc tgttgtcgaa cgttcgaaat ccatcagtcg
    28861 cgtattccaa gatcctaaac tagggacagc cgttcgttta acggtagaag aaaatcttgc
    28921 tcttgctatg aaacgaggaa aaaaacgcgg cttcttccgt ggtgtcaaac cacaagaccg
    28981 aacatttttc aaagaccagc tggttcggct aaatttgggg ttggaaaatc gtttatcctc
    29041 agaaatcggt ttgctgtctg gaggacaacg tcaagctatt acgttattga tggccacatt
    29101 acagcgtcca gaactgatct tattagatga acacactgca gcgcttgacc ctgctacttc
    29161 caaaacggtg atggaattga cagaaaagct gatcactgag cagaatctca ctgcttttat
    29221 ggtcactcat gacatggaag acgcgatccg ctatgggagt cgactgatca tgctgcatca
    29281 aggaaggatc gtcgtagata tccagcaaga ggaaaaacag cagctcacag tgccccaatt
    29341 attagcgttg ttcaaacaaa acagcggcac ggcattaaaa gacgatcaag tacttttatc
    29401 ttcataatca gaaagaggat gggacaaaaa atgaacgccc accctcttct tttttctctt
    29461 tccttctttc gtttttgctt tatgtatcaa ccgttgtttc ttcgaaaact attttgttct
    29521 ctccttcttc ctgatagaat ggatgggaaa ggagcttgat gccatgacaa cgaaagacaa
    29581 ggtacttcaa ttattgaaaa agacaccaga ttttctttcc ggagaaaagt tggcacagca
    29641 actggaagtt tcccggacaa gtatttggaa agccatcaaa gaattggaaa aggcaggcta
    29701 tcgttttgag cacgggccta cagggtatcg ttacctgccc tctgacgtac tagatgcaaa
    29761 agaaatccag caagagctac ggtcgtttat tcctgatttg aaagtacaaa cgatgttgac
    29821 ttctgaatct acgatgaaag atgccaaact ggcagcagct caaggagatt ctgtttcgaa
    29881 gttatttgtt gctgatacac aagaacaagc tcgtggacgt tttggacggc cttttttcgc
    29941 tgaacctgga cgaggaatct acatgagtct cttgctccat ccagacaaac ggtttgatga
    30001 attgcctcaa tatacgatca tcgcagccag tgcctgtgtg aaagccattg aatctgtaac
    30061 cggtcgttct gcttcgatca aatgggtcaa tgatatctac atggatggaa aaaaagtctg
    30121 tgggatttta tctgaagcag tcagtgatat ggaaagcggc aggatcagtc atatcatcat
    30181 tgggatcggg cttaattttt ctgttcctca aaatcatttt ccagctgatc tccaagaaaa
    30241 agccatctct gtctttcccg acggacaagc tagtgtcaca aggaatcagc taatcagcaa
    30301 aatctggcaa gaattgttca cttatctttc tcacccagaa aaagcgttgg acttctataa
    30361 agaaaaatca ttcgtattgg gaaaaaccgt gtcgttcaat caaaaaggaa cgacttatgt
    30421 cggtcttgca aaagaaatcg gtgatcatgg cgaactgatc gtccaaacaa atgaacagaa
    30481 gatgagtttg tcttctggcg aaatcagtct cacatctatc cgtacgcaat aattatcaga
    30541 tttcacaaaa ggacacctga tctcatacag tgtccttttt tctgaattct cttcaaaaga
    30601 ctctccttat tctatcagct gacagtttct ctatcctcta gaaaaaggta ttctaaaaat
    30661 atctataaag catggtacac tttctttaaa tgaatcaatg caaaaaacaa gagagtcatc
    30721 agtttacacg atcaaaagag atagcaggcc gcttcctttt tcaatcttat agatagacca
    30781 tgggtaacga taaaaacaaa aaagaacttg cgacttattt tgccgtaagt tcttttttag
    30841 ctagtgcctt aatctcttaa tcccaacaaa cgtctcgcat agatcacctg accagagtgc
    30901 atagcagcat catcaataat agaaacgaga cgcattcctc gtgtgactgg tggtgtccag
    30961 ttctcgtcaa ttacttcatc tagctcggaa ggttccagcc ttcttaagta agaaatagca
    31021 aaatcagcag ctcctcgcaa gtatcctagc agatcttcta tttgattagc ttggatctgc
    31081 tttgcttgtt ccagactatg cttccaatct tgttcgttat ctgctatcgt catcggaaac
    31141 cggctttccc agtcttgact atgccaaatt ggagaagttc ctgctaaatc agcaatctgg
    31201 aaatctaatt ctcgagcggt atgccaagcc agccaagtca ttgatttgat ctgcggggct
    31261 ctatcttcca ctggaaaacg attggcctcc tcaatagaga cgccttcaaa caaacgttca
    31321 aaccgatcgt atgcccgatc cacactctcg catagcatat ctaaataatc catttatttt
    31381 cctccttttt atttatccta cttacttttt cctaattcta ggatacctac ttatatgtta
    31441 ctacacaccc ttatttttta cactttaaca gtcaccatca tcaatagttt ggagattgaa
    31501 agaaaaagat agtgtcgaac tatcaattga acaagaagca acaatatgaa cgtttcatta
    31561 atattcttta aataaacaat gatttttcaa acgaacagtt taagactagg agaaaaagag
    31621 tataatagga aaatgtttga aaggaaagga aggggaaaaa tgaaaaaaag ggcaagttta
    31681 ctagtcctag ttgctttgtt gttcaatatc ttttcgtatg gtgtggccgt ttcagcggaa
    31741 acatacattc ctaaagaccc tgggaatatt tataatgatc taactggtaa ctatggcttg
    31801 ttaggaattg ctagtcaatt ccacgtattt gctaaaaata agacaactgt gaatgcacat
    31861 acagacggaa atatagcaac acatgaactt gatgcaaaaa ataattttgg tacagagctt
    31921 catgaaggtt tattgaacca agaaataaat tatatacaaa aaactgattc tttcgctaat
    31981 tcttcaggca tacctacagg tagcacaaga atgaataaat ttgtcgttgg cagtaacgtg
    32041 gatgtagcac ttcaagatgg tcatcctaca gtcaatggat ccaggcttga tcacttaact
    32101 ccagatgatt tttataaaga tcgtgctggg catgaatata ttgatttcga gcaggaattt
    32161 gctaaattga atgtagcagc aaatgatctt gcaacgatca ctccagctaa aacatatact
    32221 gctgcagatt ttccagacat gaacaatcgg acaatcgatt taactgattt atcagatacc
    32281 gggtttttat tggtaaatat cgatgcagaa gtgttaacga tgaatacgcc acttcaaatc
    32341 attaatccaa atgatcaagt agttgtattt aatgtcatca acagttcttc tgcattgaac
    32401 gtacagtcac caatcaaata caatgatcgt tcaaatcatg aaacagaaga tttttcggat
    32461 gcgaatatct cttggaattt tggaaatgag atgactgatt tgacaatcag tgctcctttt
    32521 caaggaacaa tccttgcgcc taatgctact atccgcatta ctcaaaacca agacggaacg
    32581 attataggaa caaatgtgat tttagatgca gctaccaatc gttgggatcc taacgaaata
    32641 tttatcagcg acaatggtac agatacgaca gacacaagcg acagtacaga tacatctacc
    32701 acaagtgata gctcagattc atcaacttca agcgacagta cagatactac ctctaccaca
    32761 agtgatagca cggatacatc agcttcaagc gacagtacag
//
LOCUS       DACECY010000029        32789 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.030, whole genome shotgun sequence.
ACCESSION   DACECY010000029 DACECY010000000
VERSION     DACECY010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.030"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            119..1045
                     /gene="cysK"
                     /locus_tag="IWO38_001438"
     CDS             119..1045
                     /gene="cysK"
                     /locus_tag="IWO38_001438"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ0313533.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            1281..1727
                     /locus_tag="IWO38_001439"
     CDS             1281..1727
                     /locus_tag="IWO38_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0313534.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2000..3352
                     /locus_tag="IWO38_001440"
     CDS             2000..3352
                     /locus_tag="IWO38_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAQ0313535.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            3523..3810
                     /locus_tag="IWO38_001441"
     CDS             3523..3810
                     /locus_tag="IWO38_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313536.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            4036..4479
                     /locus_tag="IWO38_001442"
     CDS             4036..4479
                     /locus_tag="IWO38_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0313537.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4485..5669)
                     /locus_tag="IWO38_001443"
     CDS             complement(4485..5669)
                     /locus_tag="IWO38_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ0313538.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            5937..6635
                     /locus_tag="IWO38_001444"
     CDS             5937..6635
                     /locus_tag="IWO38_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ0313539.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            6642..7319
                     /gene="nth"
                     /locus_tag="IWO38_001445"
     CDS             6642..7319
                     /gene="nth"
                     /locus_tag="IWO38_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ0313540.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(7445..7978)
                     /locus_tag="IWO38_001446"
     CDS             complement(7445..7978)
                     /locus_tag="IWO38_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313541.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(8275..10656)
                     /locus_tag="IWO38_001447"
     CDS             complement(8275..10656)
                     /locus_tag="IWO38_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ0313542.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(10646..11293)
                     /gene="recU"
                     /locus_tag="IWO38_001448"
     CDS             complement(10646..11293)
                     /gene="recU"
                     /locus_tag="IWO38_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ0313543.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            11357..11896
                     /locus_tag="IWO38_001449"
     CDS             11357..11896
                     /locus_tag="IWO38_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ0313544.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            11986..12405
                     /gene="gpsB"
                     /locus_tag="IWO38_001450"
     CDS             11986..12405
                     /gene="gpsB"
                     /locus_tag="IWO38_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ0313545.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            12427..12794
                     /gene="rnpB"
                     /locus_tag="IWO38_001451"
     ncRNA           12427..12794
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IWO38_001451"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13003..14169
                     /locus_tag="IWO38_001452"
     CDS             13003..14169
                     /locus_tag="IWO38_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ0313546.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            14215..15711
                     /locus_tag="IWO38_001453"
     CDS             14215..15711
                     /locus_tag="IWO38_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ0313547.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMEDLYFNVYQVK"
     gene            15792..16475
                     /locus_tag="IWO38_001454"
     CDS             15792..16475
                     /locus_tag="IWO38_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0313548.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            17120..18310
                     /locus_tag="IWO38_001455"
     CDS             17120..18310
                     /locus_tag="IWO38_001455"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ0313549.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKRM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLVTYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKAGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            18412..19365
                     /locus_tag="IWO38_001456"
     CDS             18412..19365
                     /locus_tag="IWO38_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ0313550.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWDLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDAMTKDMLEKLKEKLVETV"
     gene            19434..20783
                     /locus_tag="IWO38_001457"
     CDS             19434..20783
                     /locus_tag="IWO38_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0313551.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETRGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(20965..21288)
                     /locus_tag="IWO38_001458"
     CDS             complement(20965..21288)
                     /locus_tag="IWO38_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313552.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPQALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            21417..22580
                     /locus_tag="IWO38_001459"
     CDS             21417..22580
                     /locus_tag="IWO38_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313553.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKLAFDSGTVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            22650..23999
                     /locus_tag="IWO38_001460"
     CDS             22650..23999
                     /locus_tag="IWO38_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0313554.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24044..24475)
                     /locus_tag="IWO38_001461"
     CDS             complement(24044..24475)
                     /locus_tag="IWO38_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0313555.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMEKSLA"
     gene            24863..27505
                     /gene="alaS"
                     /locus_tag="IWO38_001462"
     CDS             24863..27505
                     /gene="alaS"
                     /locus_tag="IWO38_001462"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ0313556.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDAPTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27705..28301
                     /locus_tag="IWO38_001463"
     CDS             27705..28301
                     /locus_tag="IWO38_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0313557.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            28423..29571
                     /locus_tag="IWO38_001464"
     CDS             28423..29571
                     /locus_tag="IWO38_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ0313558.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFDDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(29609..30022)
                     /locus_tag="IWO38_001465"
     CDS             complement(29609..30022)
                     /locus_tag="IWO38_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ0313559.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            30101..30532
                     /locus_tag="IWO38_001466"
     CDS             30101..30532
                     /locus_tag="IWO38_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ0313560.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(30631..30864)
                     /locus_tag="IWO38_001467"
     CDS             complement(30631..30864)
                     /locus_tag="IWO38_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ0313561.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            31046..32716
                     /locus_tag="IWO38_001468"
     CDS             31046..32716
                     /locus_tag="IWO38_001468"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ0313562.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGIIAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNEWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
BASE COUNT        10907 a         5646 c         6841 g         9395 t
ORIGIN      
        1 attaccatat atggtatatc tttgtctttt ttttgctagt caaagacgaa tcactcgcca
       61 taaatgaaca gattgtatta tgataagtaa gaagagcaaa gacaaggagg aattgaagat
      121 gacagctatc tataactcag tcaccgaatt gattggcaaa acgccaatcg tcaaattaaa
      181 taaaatcgta ccagaagatt cagcagatgt ttttgtaaag cttgagtttt ttaatccagg
      241 aggaagtgta aaagatcgta ttgctttaag tatgatagaa aaagcagaac acgatggtct
      301 actgaaacca ggtgatacta tcattgaacc tacttctgga aatacaggaa tcggattgtc
      361 gatggtagga gttgcaaaag gatataaagt aatcatcgtg atgcctgaga cgatgagtat
      421 cgaaagacgt ttattgatga aagggtacgg cgcagagttg attttgactc ctggtgcgga
      481 tggtatttct ggatcaatca gagaagcaga acgtttggca aaagaaaacg gctacttttt
      541 accattacaa tttgaaaatg aagcaaatcc tcttgtgcat gagaagacga ctggtcctga
      601 gatacatcaa gcatttggag taaatggctt ggacgcgttt gttgcaggta tcggtacagg
      661 aggaacgatc actggcgctg gccgagaatt aaaacgtgtg tatccgaaaa tcgaattaat
      721 cggggtagaa ccagcagaat ctgctatttt agaagggaaa gaagcgggac ctcacaaaat
      781 ccaaggaatc ggtacaggtt ttgttcctaa aacactggat acttctgtgt atgataaagt
      841 cctttctatt agtggagacg aagcgatgga aacagctaga gaagttgggc gaaaagaagg
      901 gattttagta ggcatctcat ctggcgctgc aattgctgcc gcgctaaaag tcgcaaaaga
      961 attaggaaaa ggcaagaagg tattagctgt ggttcccgac aatggtgaac gttatttatc
     1021 tacagccctt taccaagaga tataaggcac ttggtttttc tccagatagg tttatgtaaa
     1081 aaacttttaa agatgaaaag aacacacaaa aatttatgat gagaaaaatt atttgaatag
     1141 agacgtagaa tagtgaacgc gactgtttta cgtctttttt cattaaaata caataaaatt
     1201 gaattttctt ttcatgttaa gtggatttta aaatgtaaaa atagtatgat aagacgagaa
     1261 taacaacgtg gggtgtttac atggaaaata agttagtcga agaggttatc gaaaagttaa
     1321 aaaaagagaa cattcgaatc acacctcaac ggtacgcggt tttagaatat ttgattgaac
     1381 atcattccca tccaacggct gatgagattt ataaagcact tgaacatcgg tttccaaata
     1441 tgagtgttgc gactgtttac aataaccttc gcttatttac ttcgattgga tttgttcaag
     1501 aaatgaagta cggcgatgct tctagccgat ttgatttcag ttcaaagcgt cattatcatg
     1561 taatttgtga aaactgcggt aagattgttg attttcatta cccagggctt gaagatatag
     1621 aaatggctgc aagcagactg actgattttg agattaatga acatcggttg gaattgtatg
     1681 gactctgtcc ggattgtaaa gagtcagcaa aaaaggcgca aatgtaaaaa cagtttctac
     1741 aagtgttata cctaaaaaaa gctagctgtg taaaaaaaac gctagctttt ttattttttt
     1801 gtcttttatt cacaatctta gatataaaag tgaatattat taaaataaga atcttgtaga
     1861 tattgggttc ttatgcttga aaaggaatga aactacaaac aaacctttgt gaattcacaa
     1921 gcattaaact gttgtatccg ttttatttct gttatagtag ttttgtcgaa ggacaaaatg
     1981 ttaatcggag ggaccataaa tgaaagtagt agtaattggc tgtactcatg caggtactgc
     2041 agctgtaaaa agtattttaa aaaatcatcc agaagcagaa gtgacagtat atgaaagaaa
     2101 tgacaatatt tctttcttat cttgcggtat cgcactttat gtcggcggag tagtaaagga
     2161 cccagcaggc ttgttctact caaatcctga agaattatct tcattaggag caacagttaa
     2221 aatggaacac gatgtaacag acgtcgatac agaaaataaa acagtaacgg caaaagatct
     2281 gaaaacagga gaaacagaaa cggtttctta tgataaatta gtgatgacaa ctggttcatg
     2341 gcctattatc ccaccgatta gtggaatcga tgcgaaaaat gtactcctat gcaaaaacta
     2401 caatcaagca aatgaaatta ttgcacaagc gaaagaagca aaacgtgtag taattgttgg
     2461 tggtggatat atcggtattg aattagtaga agcttttgtc gaatctggaa aacaagtgac
     2521 attgattgat ggattagatc gcatcttgaa taaatatcta gataaaccat tcacagatat
     2581 cttagaaaaa gaattgacag accgcggtgt tactcttgcg ctaggcgaaa atgttactga
     2641 atttatcaca gatgaagaag gaaaagtgaa acaagtagct acaccaacag atacatttga
     2701 tacagatatg gtcattttat gtgtgggctt ccgtccaaat acaaaactga tcgaaggaaa
     2761 agtagataca ctgccaaacg gggcaatcaa agtgaatgaa tacatgcaga caagtaatcc
     2821 agacattttt gcagctggag actctgcagt agtcaactac aatccaagtg gcacgcaaaa
     2881 ctatattcct ttggcaacaa atgccgttcg tcaaggtttg cttgtgggaa ataacttgac
     2941 agatcataaa atggcgtatc gtggtactca aggaacttca ggactttacc tatttggctg
     3001 gacgatcggt tcgacaggtg tgaccaaaga aagcgcaact ttgaacgatc tagaagtcca
     3061 agcgactgta tttgaagata attatcgtcc agagttcatg ccaactactg aaaaagtaat
     3121 gatggagcta gtctatgaaa aaggaacgaa tcgtatcgta ggtgctcagt tcatgtccaa
     3181 atacgacatt acacaatcag caaataccat gtctttagct gttcaaaata aaatgactgt
     3241 agaagatctc gcactttcag atttcttctt ccaaccacat tttgaccgcc catggaatta
     3301 cttgaaccta cttgcacaag cagcgctagg cgaaatcgaa actgcacaat aaagaaaaag
     3361 ggttgtgaca caattttgtc acgaccctct gtcttttttt gtgaaaagta caattagttg
     3421 tctttcttcg aattttttgt cacaataagc agcaaagata aagctgttac ataaaagaac
     3481 aagcagcttt atttgatttt gaaaagaaag gtgagaaaga agatgaataa agaatatttt
     3541 gtccaacaag atgcattttt agaagattat aaaagtgctt cgaagaaatt aacgatcaat
     3601 attgatgagt tggcagatca agtactctct cagttgaatg aaacgcaaga ggaatattac
     3661 acgatgccag caaatgacgc caaagataat gtttctcatc gttttccttt cagtagacgc
     3721 atctactcag atgaaaatga cggcacattg aatacagagt acacatatga aggtcagcca
     3781 tatgtgctag aaactgatga tgatctttaa agacagtttt tgaaaaggcc gctcatcctt
     3841 aagtgatgac aaaaaatcat cacaattacc gatgactgtc tctttactat cttttaatca
     3901 gaaaaagggg ttatgacaag ctttgtcaac cccttttttt cttggtttca gattgggttt
     3961 tcctacaaaa tatttttatc tttgttgaat aggaaacaaa aaaacaatat aatgaaagaa
     4021 aaagaaggtg tagaaatgga taaaagacaa ctgctaatag aagaatatct ggatttagct
     4081 aatgagatta ttcatcgtaa taaaccagaa atggaaaaag aatttgcagg atttactcta
     4141 aatgaactag aagtgattga acatatcggg aaaatccctg atcctaacgt gacaaaatta
     4201 gcgaatgcta gttacatgac tagaggagcc atcagtaagc taacaaaaaa actgatagct
     4261 agagggatca taaacactta tcaaagcgaa gagaataaaa aagaaatcta ttttcaattg
     4321 acgccaatcg gacaaaaaat aaggaataga cacgagcaac tccatcaaaa atgggtacag
     4381 cgagatgcta ctgtatttga aaatatgtca aaagaagaat ttgacacagt atttcgtttt
     4441 atcggtcgat acagagaatt tatacgaagt agcaaatgaa gctactattt tttattgtag
     4501 tgataatagg caaaaatcat aagcaaaaat ccgaataccg tcggaagaaa tggtgctgca
     4561 gcataaagct gtcctgcgat tagcgggccg atgacgcgcg atagagattg tatactttgg
     4621 gtaccgccca ttacaagacc ttgctggtta tctgagacag aattggatag ttggccattg
     4681 aatacaggag taaaaataga atcaccgaaa ccataacaga taatcccaaa taaaaacaaa
     4741 atcggcagta gaagaattcc agataatgtg atgaatagat aggcgaggat ttcactgaac
     4801 atccctattc ttgtgatctg ctgttctgaa aattttttga gcaatcgagg cataatgaac
     4861 aattgcgaga cagtatccaa gattccaatc aaagagaagc ttaaaccaac caacccagcc
     4921 ttccactgaa aggcgtcaat cgaaaattga gaaaagatag attgaagaga gccagctgcc
     4981 aaccaaacag caaaacctgc tgtcaataaa ggaataacgg atgaaatctt gaataattgt
     5041 tgtaattggt gaaaaggtct tacatgggag aaacttaaat ctactgaacg tttttttatt
     5101 ggaaggcttt ctagcataaa tgtgtagcca taaacggcat ttaagaaagt aaagagtgct
     5161 ccaataaata taggaacaga attccccaac tcagctaata gaccccctat aataggaccc
     5221 agcgttgttc ctataccgac taaagcaccc atccatccaa aaactttggt tctttcatta
     5281 ggttccgtga tgtcggcaaa gtaagcatat aaggtggaga tttctcctgc cgtcaatcct
     5341 tcaatgatac gaccaaggaa caacatccat atcgaatttc ctaaaccaaa aagcaaatat
     5401 ccgatacttg atcctaataa actgataata aggatcggat gacgcccaaa atgatcactt
     5461 aagctaccga gaatgggggc agataagaag gcagctagag cataaacaga catgagcaaa
     5521 gtgatataga aagcttgctg atgtatggag tgagaataag gcagcgttaa aaacggaagt
     5581 acaggactga cgatagtaaa tcctaatcca gtgaataaga cagagacgta tccaaaaact
     5641 ttagaatgtt tatttgtgaa actgtacatg taaaaactcc tttatgcaat aatgttaact
     5701 tggaaacaaa aatagtgtat agtaattttg tttccttgtc aacattctaa ggaacagcga
     5761 aatctactta atttatttat tccgaaaagt gaagatacgc atcaaaaaca tgaacaaaca
     5821 aaaatcaagt agacgacatt gtaaatctga ccgatcagtg aagtaaatag aaaagaaacc
     5881 tgttcttgct tcccatgttt tttttacgta tactgtgatt gagcggagga gaaatcatgt
     5941 tgaatttaga aaattactta agcgctggac aaacagtcat atctaatctt ttacttaaaa
     6001 attatcatag gatcggtcta caatctgaag aatttttatt tgttctacag ttgcatatgg
     6061 cacagcttga aggagatact ttccctgatt tgcagatgat tgctatggat atgggaatca
     6121 aacaagatca gatttttcaa atactggatc gtctcgtaac aaatggattt atcaagatag
     6181 aaacgacgat tgtcaacggt aaaaaagcag atcgatataa tctttacccc atctatgatc
     6241 tattaggtga gtatttaaaa acacaagaaa aaaaatacga agaaaaaaca caagaaaaag
     6301 aaatccagtc agtgtatcaa ttatttgaac aagaatttgg acgcccgctt tcttcgatcg
     6361 agttccagcg gatcggacaa tggctagaag aagaccatta tcagccggaa attttgaagc
     6421 tagcactgcg ggaagccgta ttaaatcagg cttatagttt taattacgtt gaccgcattt
     6481 tgctatcgtg ggaaagaaaa aatctacgta caaaacaaca agtggaagaa gaccaaaaac
     6541 gaagaaaaca gcagcttctt caaagagaag tagaacagtc agctcaacaa gaagaattgc
     6601 cgaaagtctc actgaaaaat tggttggagg agtagcggaa tatgttaagc aaacaaaaaa
     6661 caatggaagc tctagaaacg atgtatggaa tgtttcccga agcacatgga gaactcaagc
     6721 acaataatcc atttgagtta ttgattgcag tgatcttaag tgcccaggcg acagatgtat
     6781 cagtgaataa agcaacgcca gatttgtttg cctcttttcc gacacccgat gcgttagcag
     6841 aagcttcaat cgatgaaatc attcttaaaa taaaaacgat tggtttgtat cgaaataaag
     6901 caaaaaacat caaagcgtgt gcacagcagc tgattgaacg tttcgatggg caagtgccaa
     6961 cgtcaagaga agaattgatg tctcttcctg gtgtaggcag aaagacagca aatgtcgtat
     7021 tgggggatgc attcgggata ccagcaatcg cagtagatac ccacgtggaa cgtgtctcaa
     7081 aacgcctgag gatctgcaaa ctagatgcta cggtcatgga agtagaagaa acactgatgc
     7141 ggaaagtacc acaagaatta tgggtaaaga cacatcatac actgattttt tttggtagat
     7201 atcattgcac ggcaagaaat cccaagtgcg aggtatgtcc tttgctatca atatgtcaag
     7261 atgggaaaaa tcggatgcgt ctaaaagaaa aggcgctaaa aaagaaacca cgcctgtaga
     7321 cgtggtttct ttaattgcaa ataaatgtat gaattatctt gttattatgg agaaaagctg
     7381 tttcgtttat cttttttgca tgaaaaagta aatgtacata ctcagtagct aggatggtaa
     7441 cttattacaa tttattgcga taaaaataaa agttgttatt tttattggga aaatacccga
     7501 tgtagaaaat aatgccttcc aatataccta taaaataagt ccagtattcc aaaggattat
     7561 ataaataagt gacgtatcta tctaaaggtg taataaaaat gataagtgat aataaaaaaa
     7621 taccatttat aactaaacat aaactaatgg atttgggcgg tttcaggctc atcagaaaaa
     7681 tatagattct ggtgaacaac aaccataaaa aagtgagaat cattgcatga gagcttcttt
     7741 ccataaattt tcctttaaga gtagtgctta aaagtacaga aaaaataata aatgcaagga
     7801 taggagcaat taaagtaaat ttatttaata ataaacaaaa tttttcagct tttttattct
     7861 tggaataacg aggtattgtt ttcgataaaa aaagatggtg cagacaaatt atgataatta
     7921 agcatactat cagataactc aaaatgaagc cttctttcaa aaaattattc aaaaccatat
     7981 attgtatttt ttatttgata ataacaatgg gattattaag aagtggcact aaataaaaga
     8041 ctagtgtgta tttagccgat ttaattataa acaagtaaat ggataaagta tatgtaatga
     8101 taaaagaaaa atggattttc taatttttaa gctaaatgtt agatcacaca tgtatatgct
     8161 ggatagtctt ataaaaaatc atgtaataca gcttggtaca tgatttttat ttgtagctgt
     8221 tttttacata gacacaaaaa agaaaccacg cttgtagacg tggtttcttt tttactatct
     8281 gtttccagaa gctgaggtgc tgctggatgg tgtatggtca tttgcagcat ccgctccgct
     8341 agttgctggc tcactggaag aagcaggagg agcagatgaa gacggtgtcg agctttcttc
     8401 ggatgaagaa gaaggaaccg ttgttgaaga cgctggctcg cttgtttctg ctgtcgtgct
     8461 ttcttttgag gactcagcag ttgattcgct ttgactagta gagctagagc tttgagtcgt
     8521 tgtctctgtt gttgagcttc caggagtctg gacatagctg gaagatggaa tagttgttga
     8581 tggtgtaatt gcattcgatc tagctgtata ttgatctttg tagtacagtt caccgcccac
     8641 acgaattaat ccgcttggca tctcccagtc tgtatttgtc acgttagctg aaacatactg
     8701 cataagctcg cgataaacat cagaggctac gtgtgacgat tctgaagtga caggtgtcat
     8761 ttttttattg taacctgtcc acacggaaat cgaataattt ggcgtatagc ccgcaaatag
     8821 gatatctgga tacaccccag aagaaatacc aagttttgca tactcgtcat ctgtgtaatt
     8881 ggatgttccg gtcttaccgg cttgatataa cccagcgatt tgcgcattcg taccagttcc
     8941 ttcagtgatc gtatctttta aaatgtctgt gatcatataa gctgtttctg gagacatggc
     9001 tgtttttcca tctggctcat aagtttcttc tgttccgtct tggaagacga ttttattaac
     9061 atattgaggt ttgtaataag tccctccatt ggcgaaagcc gcataggctg ctgccatttt
     9121 taaggaagaa gctccgtatt ttgtcccatc ttgttcttcc gtattactag agatcgcatt
     9181 cgattgatgg atcgtactgt attcgatccc aagatttttt aagaaagaag ccactttatc
     9241 tgaaccgact tcattgaata atttgactgc tggaacatta cgggataagt aaagtgcttg
     9301 gcgtaaagta atggtgccca tatattgatt atcccagttt cctacaggag tcgaagtccc
     9361 ttcatagtta tacggtgcgt cagtaatagt ttttccagta gaatatttca aatattcaaa
     9421 agctggtccg taatctgtga caggtttcat cgtagaacca aaatcacggg aagtattcac
     9481 tgctaagttg ttgcctaaag ttacgtcttc tgcgatgtga cgtccgccaa tctgggcttt
     9541 tactttcccg gtattggtgt cgataagggt ggaggcaact tgcatctcgt cgtctgggta
     9601 agagacatac tgatccgtgt tgacgatatc atatagtttc ttttgggcgt ccagatccaa
     9661 attcgtataa atctctaaac cgtctgtgta gacattctta tctgtttttt cttgtacttc
     9721 attgatgact tctttgacat aattatcgac gatcttagta ttgtcatcgg attgagttag
     9781 ttcttgcaga ccatccgtta ccggtacatt aacagcttga tcatattctg tttgagagat
     9841 tttttcgttt tgaagcatcg tatacaaaac agtatctcgt cttttcttcg cctgatctgg
     9901 ataaacataa ggatcatagg cggaaggggc ttgaggcatc ccagcaagca atgctgtttg
     9961 cggtaaagaa agttctgata atttcttacc aaagtacatc tcagaagctg tctccatccc
    10021 gtacaagccg tttgacatat acactttatt tacgtagtag gtcaagatct cttgtttcga
    10081 ttttttttgt tccaatctta cagccatcca cgcttcttgg gccttgcgtt tcagagtctg
    10141 atcctcggca ctggtggaga agaaggaaag tttgatcaat tgctgtgtca aagtacttcc
    10201 gccttgcagt ccgccagagg taaagttaga gagggcagaa ccgatgatcc gaatagggtc
    10261 aaccccgatg tgtttataaa aacggcgatc ttctaccgac acgatcgcat cttctaatgt
    10321 tttcggtaat tcattcgcag aaattttttc acgcttttct gcgcctaaat cttcgaatag
    10381 ttcgccatct tgtgtatata gtttggaaga tacggtagcg tctaattttt tatccgtcaa
    10441 ttcgggcgca tcttttgcgt aataccagaa aagtcctaca cctgctaaaa agaggataca
    10501 gaaaaatgag agtattccta aaaggatctt gataaataat ccttttttat gtgttccaga
    10561 ggactttcca gaacctttgc cagaattctt cttcacactc tttttaggag taggctgttt
    10621 ctgtcgtcta gagcttctag tttgttcatt tgccattctt gctttctcct tcaatagata
    10681 atgttgataa atagaagtca accgcgtcca aataaggaat ccgcggagaa ataccagttt
    10741 tgatctctat tcctagttct tcgatcaaag ataaaggtag tgatttgcgt cctttttcct
    10801 tttgggcgtc ccagtaagca atcaaatgaa cactattcaa ataaaaacag cgcttcaaag
    10861 acgaaaacca taacaagaca aagcagaccc cattttgttc gaggcatgct ttcatatgtt
    10921 cgatttgatg ggcatggaag tttttaaagg gaaaagatgt tttgttcttg gtttcttttg
    10981 cttcaaagtc caagtaaaag cctcgataga cgccattata gtctgtagtt gaggcttggg
    11041 taaagtaggc ttccttgatc acagcagcac ttctcttagg atagtctact ttcacgattt
    11101 ggataggggt aggcttttta tggataaccg ccaacccgcg ggaaagataa tatttattac
    11161 tttcattgat tgcttcttca aagcgcatcc cccgattgcc gaattcagtg atctgatttc
    11221 ttttttcttg aattttttcg acgggagagg cattcttata ataaggttgg ccatttggat
    11281 agcgcaatac catctttaat cacactccta ggccacatta tagcaaaaaa gcggatagaa
    11341 agaaaaggaa tctgagatgg ataagtcaaa agtaatctat gtgtctgggt atcgaagttt
    11401 cgaactcggt gtttttaaag aaaatgatcc taaaatccaa gttgttaaaa atgttttaaa
    11461 aaaagaactc catcagttag cagaagaagg gctggaatgg gtacttgtca gtgggaattt
    11521 gggcgtcgaa ctatgggctg tgcaaatagt agcggaattg aaagaagaat atccggaact
    11581 atctcttggt attttgtatc ctttttctga ttttggtaat aactggaatg aagggaacca
    11641 agaaaaaaaa caattagcag aacaattagc agattatgtc gaagctgtta gccatcaacc
    11701 ctaccaatca cctagtcagc taaagaatca cactcgtttt atacttgagc atacagtggg
    11761 ctgtctgctg atctatgatg aagaattccc tggaaaaacg aagtattttt ttgaagatgc
    11821 acagaattac caagcagatc atccctacga aattcggctg atcagtatgg acgatcttca
    11881 aaattttagt gaataatcga tattgtgttt gatttatcaa cttttttcct atacaatgaa
    11941 aaaggtagaa atgttattac gtaaatgaaa actagaggtg taaatatggc gaatttggtt
    12001 tatagtccta aagacatttt acaacaagaa ttcaaaacaa aaatgcgcgg ctatgatccc
    12061 gttgaagttg atgaattctt agacaatatc atcaaagatt atgaagctta cagcaaagaa
    12121 ctccttgctt tacaagaaga aaacgatcga ttgagtgcaa aagtagctca attatctaaa
    12181 actcaaggag cagctcaaac acgcgtgcaa caaacagagg caccaaagag cgcagcagtg
    12241 accaattttg atatcttaaa acgcttgtca aatcttgaac gtgaagtatt tggtaaaaag
    12301 cttgatcaac aagcaagtgc agtcaaacca gcacaaccta atccaaataa ctacacaaat
    12361 gcagacacaa gcttagatga taatgaaaaa acacgtcaat tttaattttt gattaatgaa
    12421 tttgcggttt tcgggtaatc gcggtctgac ttgatcaggc tgaggaaagt ccatgctcgc
    12481 acaggctgtg atgcctgtag tgttcgtgct tagcgaaacc ataagctaag gtactctttg
    12541 gagtaacggc aggaaaacaa gctaaggctt cggctatgct taagtatcct tgaaagtgcc
    12601 acagtgacga agcggctggg gaaacccatc cggtggaacg cggtaaaccc ctcgagcgag
    12661 caacccaaac aatggtaggg gcgctttttg tctggaagaa gaacaggaca aaaaggcagt
    12721 ataactgcag atagatggtt accgctttgt tttttctccc tggaaaagca aagtacagaa
    12781 catggcttat agaaaaccgc aaacatcagg gaattaggtc gtgacaaaag taatgcgtta
    12841 taagaaacaa gaaaaacatt tgaaagctat ttttcaatac ttcagatgaa tcgttcttat
    12901 tacaaaacaa acttttgtat caccggttat tcggaatttg agtaaaagtg tggggtagga
    12961 ctgatgtccc acctcctttt tttataaata aggagattgt gtatggaaaa aaacaggaag
    13021 ttcaacctcg tcgcaactgc ggcaagcggg ctagaagcac tggttggtaa agaattaaga
    13081 gatcttggaa tcgaatgcca agttgagaac ggacgtgcac gttttgaagg aacaatggaa
    13141 acgatcgcta cagctaattt atggttacgt acagcagatc gagtaaaaat cgtggttggg
    13201 gaattcgatg cgtatacgtt tgatgaattg tttgaaaacg taaaagcatt gccttgggaa
    13261 gattttcttc cactagatgc agcttttcct gtagctggaa aatcgatcaa gtcgaaacta
    13321 tatagcacac ctgattgcca agcgatcaca aaaaaagcaa tcgttgaacg tctccgtacc
    13381 tattatcacc gtccagcttc tgtaccactg acggaaactg gcgcacattt tcagttggaa
    13441 gtagctttgc tgaaagacca tgtgatggta actctggata cgacaggacc aagtttattc
    13501 aaacgtggtt atcgcttaga aaaaggtgga gcaccattga aagaaaacat ggcagcagca
    13561 cttattatgt tgacaaattg gcgaaaagac cgtccatttt atgatcctgt atgtggttca
    13621 ggtacgcttt gtattgaagc agccttaata ggccataata tcgcaccagg attcaatcgt
    13681 gagtttgctt gcgaatcttg ggattggttc agtcaagaaa tcatggaaaa tgttcgatca
    13741 gcagccgaag aaaaagcaga ttatgatatt gaattagata ttactggttc agatatcaat
    13801 ggacggatga ttgaaattgc caaagccaat gcagaagaaa tcggattagg ctcttcgatt
    13861 acctttaagc aacaagcagt caaagacttc aaaacggata aagaatacgg ggtcatcgtg
    13921 gctaatccgc cttacggaga acgtctagga gaagaagaaa ctgttcggaa attatataaa
    13981 gaaatgggcg atgtattccg tccattaaaa acatggagta aatatatctt gactagcgac
    14041 ttagcgtttg aggaatttta cggacaaaaa gcaaccaaaa aaagaaaatt gtacaatggt
    14101 gcattgcgta cggatctatt ccagtattgg ggaactcgtc cgcctagaaa aaaagagaaa
    14161 gacgaataat cttactaatt gattcattca attggatgtt tggaggaata gggaatgaaa
    14221 gaaaaagagt ttttacaaga agtaaaagaa atcaatcttc tacaacaagc tttaggaatc
    14281 cttgattggg atacacaaac aggtatgcct gaaaaagcta gtgcataccg aagtgaagta
    14341 gacagctatc tttacagcat ttatttttca aaaaaaatcg gtccaaaaat caaagaagcc
    14401 attcgctatt tttctgaaca tccggaagaa ttgtcggaag ttggaactgc cgtttttgcc
    14461 aaagtaaaag aagagtatga tttggaacat cgcgtaccgg aagatcttat gagaaaatat
    14521 acagctgccg tttcttcagc acacggacaa tggcaaaaag ctagagaaac acaacaattt
    14581 tctgattttc aacacgcatt gaatcaaaat attgaattaa caaaacagtt gataccttac
    14641 tggagaaaag atgaaaagac gaattatgat gttttgctga atcaatacga acccgggatg
    14701 actgtcgaaa tccttgataa ggtatttgat cagcttaaag aaggaattct ttcgatccgc
    14761 cgtacgctcc aagaaaaagg gactgaacct gaaacagact ttttaaatcg gagaatgacg
    14821 aaaaaacaac agaagagatt tgtagtgaaa gtaatcgaac aacttggtta tgattttagt
    14881 cgtggtcgtt tagatgacac tgtccaccca tttatgactg gaattaatcc gaatgatgca
    14941 cgaatcacga cacgatggaa cgaaacagat ttcaaaatgg ctgtttttgg agtgatccac
    15001 gaggctggtc atggtatgta tgaacaagac atcgatgata aatatgcgta tacaccggtt
    15061 tatgaaggta catcaatggg tatccatgaa tcacaatcgt tgtttaacga gatcattatt
    15121 ggaagtagtc gcagcttctg gaaaaagcaa tatcctttct ttcaagaatg tgcagaagga
    15181 acattcgatg atattccttt tgacaaattt tatcgttcat tgaaccatac aaaatcaagt
    15241 ttggttcgta tcgaggcaga cagtttgact taccctctcc atatcatcat tcgttatgaa
    15301 atcgaaaaaa tgattttcaa tgaaggggta gatgttgctg aactacctga aatatggaat
    15361 caaaaatatg aagagtatct gggaatacga ccaacaaatg atgtggaagg catcctacaa
    15421 gacgttcatt ggtcaggtgc aagctttggt tattttccct cttatgcatt agggtacatg
    15481 tatgcggcac aattacttca ttctttgcag caagaaatag atgtggaaaa tgttctagcg
    15541 tcagacgatt attctccaat caaaaactgg atgtcagagc atatccatca atatggtgca
    15601 tctagaaaac caaatcaatt aatcatggat gcaacacatg aaccgcttaa tccgcaatac
    15661 ctgatcgact atatggaaga cctgtatttt aatgtatatc aggtaaaata aaatacaatt
    15721 tacaaagata gctgaaattc atctaatcta tagatgtttt cagctattta cttaagaaag
    15781 aagtggaacg aatggataaa ttacaagtga tcgctgatta tagtatggac caattagcgc
    15841 atgatcaaac aggacatggg agcgatcata ccaaaagagt tgtcaagtta gctgaaaaaa
    15901 ttttggagac ggaaccgcag gcagaccgct ttgtgacgct tgcagcagct tacctacatg
    15961 atacgattga tgataaagtc gttaaaaatg agaacgaggc aaagcaacag cttcgtgtat
    16021 tccttcgctc tttgccaata gtagaggaac agatctcgat gattttttcc attatcgaaa
    16081 atatgtcctt ttctaaaaat ctctctgaag cggccgaact atctctggaa ggtaagatcg
    16141 ttcaagacgc tgatcgtatc gaagcattag gagcaatcgg cattttgcgt actgcttatt
    16201 tcggcggtgg acatgggcac ccgatttttg atcctgaact gtatccgcaa acattcaagg
    16261 acaaagaaaa ttaccgaaaa ggcacgacag tgatcaatca tttttatgaa aaactgttct
    16321 tgctgtctga taaaatgaat actgattacg gatatgaaga agccaaacga agagaaacct
    16381 tcatgcgtga attcttagaa gaattttttc tggaatggtc tattgacgat gaaaatttta
    16441 ctccagacaa ttggatcaaa aataacaaaa aatgatggag gttgtgacag aagtggtcag
    16501 ctccaagata tttcggatca ttttccgtaa aatttacttc tggaacactg tttaccaaga
    16561 ggtcatgagc ctgccgttca gctccgagtc acaaggaaca atttcaaaaa atagcttctc
    16621 atgtttgatg aatattgtaa cttgtgattc ggaattggca ggagaacacc gtttattcgg
    16681 aagaatagga ctgtgacaag acttttgtca cagtcctttt ttcgaattca acaagtaaaa
    16741 cttttaagaa atattcagag agtaaaatta aaaattgctg ataaataaag atggaagttt
    16801 tatgtatttt tattggtcta tgcaataaat aaattaaata ttcagaatac aattgatttt
    16861 aacgaaaggg cttctatcaa tggttgtgtt gtatttatct gcccaggtag agtaaaatag
    16921 atcattatct aaaaggtaat tgttcatact atataagtca tttgaaagta aaaagatact
    16981 gtaaataaaa aatgtggtta aataattctc ataatcagtg taagcgttat caaaaatatg
    17041 gcatacttgc tttgtagatg aatgacacga tatatgtttg gtatgttctg caagacagat
    17101 gaatatggag gtggagacaa tgtcttccaa agaactaaca atgtatttag agaaagaatt
    17161 ggatcaactg aaagaaaagg ggttatacaa tacgatcgac atactagaaa gtgaaaatgg
    17221 tgcttgtatc atcgtagacg gaaaaaggat gatcaatcta gcatccaaca attatcttgg
    17281 atttgccaat cgcgaagaat tgaaaaaagc atgtattgaa gcaaccgaaa cttatggtgt
    17341 aggagcagga gcagtccgaa cgatcaatgg ttcgctgaaa atccatcagc aattagaaga
    17401 aaaaatcgca gaattcaaag ggacagaagc agcaatcgcc tttcaatctg gattcaactg
    17461 caatatgggc gctatctctg cagtaatgac aaaagaagat gctatcttat cggatgaatt
    17521 aaaccatgca tctattattg acggttgccg cttatccgga gcaaaaatca tccgaattaa
    17581 acatcaagat atgaaagacc ttgagaaaaa agcaaaggaa gcgatagaaa gtaagaaata
    17641 taaaaaaatc atgtatatca cagatggtgt tttctccatg gatggagaca ttgcacgatt
    17701 gccggaaatt attccgattg ttgaaaaata tggactggtc acctacgttg atgatgcgca
    17761 cggctcaggc gtaactggaa aaggtgcagg aaccgtcaag cattttggat taagcgacaa
    17821 aatcgatatg caaatgggca cgttatcgaa agctataggt gttgttgggg gttatgtagc
    17881 tggatcgaaa acactaatcg actggttgaa agctagaagt cggccattcc tattttctac
    17941 ttcgctaact ccaggtgcag ctgcagctgc attggcttcg attactttga tgcaagaaca
    18001 tccggaactt gtcgaaaaag tttgggaaaa tgctaattat tttaaagaag aattgaaaaa
    18061 agcaggctat aacatcggta tgtctgaaac acctattact ccagtcattt tgggagacga
    18121 aaaagtgact cagactttct ctaagaagct gatcgaacat gggatttatg ctaaaccaat
    18181 cgtttatcca actgtgcctc ttgggacagg caggatcaga aatatgccga ctgcggaaca
    18241 tacgagggaa atgcttgatg aagcagtagc agtttatcaa aaaataggaa aagaaatgga
    18301 aattatttaa ctaaatttag ttttttcgtc tatttaaaat tagagaacat gttcacgcat
    18361 atattgttaa tcatctgaag atagtagtaa aaaagatcag ggaggatcat catgaaaaaa
    18421 gtattggtga caggatgttt aggacaaatt ggttcagagt tgatcacacg actacgtcaa
    18481 gaactaggca gtgaaaatgt cattgcgaca gatatacgca tgcctgaagt cattgagaaa
    18541 aacgatatct ttgagatcct agatgtaatg gattacgaaa aaatgcgcag catagcagaa
    18601 caatatcaag tggacacatt gattcatttg gctgctcttt tgtcagctgt tgcagaagca
    18661 aaaccacaat tagcctggga tttgaacgtg actgggctgg tcaatgcatt ggaagtcgct
    18721 agagaattcc agctgaaatt ttttacgcca agttcaatcg gcgcgtttgg tccaaatact
    18781 ccaaaagatc ataccccaca agatactctt cagcgtccta caaccatgta tggggtaacg
    18841 aaggtagctg gagaactact atgtgattat tattatgtga aatatggcgt agatactcgt
    18901 ggtgtccgtt tccctggatt gatttcttat gcaaccttgc caggaggcgg tacgacagac
    18961 tacgcagtcg atatttatta ttcagcaatc aaagaaggaa agtacactag tttcattgat
    19021 caaggtacat caatggatat gatgtatatg ccagatgcga tcgatgccat tataaaactc
    19081 atgaatgcag atcctgatcg attggttcat cgaaatgcct tcaatatatc ggcgatgtct
    19141 tttgacccgg aagagatcaa agcatctatt caaaaaatca taccagattt tgaaatggat
    19201 tatgatgtgg atcctgtacg tcagtcaata gcagaatctt ggccaaattc actagatgtt
    19261 tcttgtgcgc aagaggaatg gggcttttcg ccgaaatacg acttagatgc gatgacaaag
    19321 gatatgttgg aaaaactgaa agaaaaattg gtagagacgg tataaaacaa gaaccacatc
    19381 caaaaagttt tttatacttt tgggattggc tatggtatga aaggggacaa gggatggaag
    19441 aatatcaagc cacgccagta aaagaagtaa aaatagaaaa tgaattaaaa cgtacgatgg
    19501 gattttttac tgctttatcg actgttatgg gcacagtgat cggtgcaggg gtatttttta
    19561 aagccgcaag cgtagcagaa gttacaggat ccgccagttt gcatatgttt tcttggtttc
    19621 tcggtggtat gatttctgtc tgtgcaggac taacaggagc tgaactggca gctgctattc
    19681 cagaaacaag agggatgatt aaatatatcg aacggattta cggaaataca gcagcttttt
    19741 tattgggctg ggcgcaagtc gtgatttatt ttccagcaaa tgtagcagcc ttgtcgatca
    19801 ttttcggtac acagttcgtc aatctatttg gtctttccca gtcaatgatt gttcccgtag
    19861 ctgttacagc tgctgtatcg atcctgctga tcaatttttt aggttctaaa gctggagggg
    19921 cttttcagtc gattacgctt gtctgcaagc ttatcccttt gtttgtgatc gtcattttcg
    19981 gtttgttccg acaagaagga gtagacttcc aactgtttcc gatccaagca ggagaaaatc
    20041 tttcattttt ctcagccttg ggagcgggct tgctagcaac gatgttcgca tacgacggtt
    20101 ggatacacgt aggaaatatt tcgggagaat tgaaaaagcc ggcgaaagat ttgccaaaag
    20161 caatttcttt aggaatcatc ggaatcatga tcgtttattt attagtaaat gcggtgttct
    20221 taagaaccgc atctatcgac ggagtcgcag gaaacagcaa tgcggcaagt gacgttgcaa
    20281 aaatgatttt tggcggcttc ggtggaagac tggtcacagt tggtatcttg atttctgttt
    20341 atggaaccat caatggctat acccttactg gaatgcgtct cccttatgtt atggcaaaag
    20401 aaaacaacct gccttttagc aagctctttg ctaaacttca tgataaaact aaagttcctg
    20461 tagccgcagg tatcttagag ctagtcatcg ctattggcat gatgatgatc ggtggttttg
    20521 atacactaac ggatatgctg atctttgtca tttggatttt ttatacgatg gtctttgttg
    20581 gtgtcattct tcttcgtaaa aaagaacctg atttgtttcg accatacaaa gttccaatgt
    20641 atccgtttat tccactagta gcaatcattg gagggacttt tatcgtatca agcacgctta
    20701 tcactcagac ctttttagct tcgatgggga tagtattgac acttgccggt atcccgattt
    20761 atctctattt aaaacgtaga tagctaaaac attcgaattt tattagaact gcactctctg
    20821 ccaaacaaaa tattttattt aaatgaggag tacgtggaag aaatgtcaaa agtactcgtt
    20881 aattttgatc acgtaaatgt cgttttgtct acaatgaaat agcaaagtaa aagacagaaa
    20941 tcgggctcaa ccgatttctg ccttttactt tgatttgcgt aactgaacta ttcggcgtac
    21001 gccgcgataa atatttaata aaaccaagca accagaaatg aaaatgggaa gccaaatcag
    21061 atagacaggg tagccaagca ctttagacaa agcttgtgga gtcattttat ccatttcaag
    21121 aagtgcttgc tgccaagtct gaagattgaa gactagcatg aaacccgaaa taatcaggag
    21181 aataaatcct aaataatgaa ttgtagttct ttttcttaca taagcgatcg tagcataaag
    21241 gaacagtaaa aaaataggta cgccaaaaac aaagtacata ttcagcatct cgacaccccc
    21301 tttgtgttta tcttacttta ctttgaaaga aatgagaaat aatagcctcg ttacttgctt
    21361 gaaacagcaa aggactgcta agataagaga gatacaaaga atcaaggagg agtcatatgg
    21421 caagttatat cgaaccgatt cgttttgcgc tgattgtttt tccattttta gccttagcga
    21481 tctcatctgt tttttttatt tatgaatatc gaaagtatgg aacatttcta gtaacaaggg
    21541 cagttatttt gtattctttc gttttttatt tattgtgtgc gtattttttg gtgatcttac
    21601 cacttccggc aaaggagacg gttgcccaaa tgactgggcc aaagatggag ctgcatttat
    21661 ttgcttcatg gcaacatttc agagacaaca cggtattggt cttgtctgaa cctagtacct
    21721 acctgccggc aatgaagcag agtgtatttt tggaaccagc ttttaatgtt ttattactca
    21781 taccatttgg catttattta agatattatt tccggctttc ttttatgaaa acgatgattg
    21841 ctagtttctg cctttctcta ttttttgaat tgacgcagct aagtggactt tattttatct
    21901 acccaagacc ataccgctta tttgatgtga atgatttagt gacaaataca ttgggtggaa
    21961 tcattggttt tgtcgttacg ccgttattta catttatgct tcctacaaga agacgaatgg
    22021 atgaactttc ttatgaaaaa ggaaagactg tctcattgac tcgacgcttg gtcgcttatt
    22081 tgatcgactg gacattttta tctcttatta atttggtagc ggcgattttc tttaaacttg
    22141 cgttcgattc agggacagtt acttctcata tctggtggct tttattagaa gtaggggtat
    22201 actttatgat tgtaccgttt ataaccaatg gatggacact tggcaaaaaa attgtgcgta
    22261 tccgtttggt ggaagaagac cgcgaacgaa tttctttaaa agcattgatg atccgctacg
    22321 ggtatctgta ctttcttttt tatggtttaa gctggttggg gcagttgctt cagcaaatca
    22381 tgaattcgac aacgaatacg gtgttgcaga tgagtgcagt attcatttct ctcggtgtct
    22441 tgttactgca aggtattttt attttaaata tcttgctatc atttttgaga aaaaaacgga
    22501 ttctgtttta tgaaaaagct agtcacacat atactgttag cacgattggc caaacaaagg
    22561 atgatgattc tcgaaattag ctgagcaaaa accctttgtc tgtgttataa tgccaaagtc
    22621 gtgataattt aaagagaaga ggtgcacgaa tgaataattt tgatatattc cgcttcaaac
    22681 cgtttatcaa tgaagcgtta accgcaaagg gcttcaaaca gcccacagag gtccaggaac
    22741 gtttgattcc tttaattgca aaaggaaaaa atatcattgg gcaatctcaa actggtacag
    22801 gaaaaacaca tacgtttttg ttgcctttgt ttgaaaaaat caatgcagaa aaagaggaag
    22861 tacaagttgt tattacagct ccaagtaggg aactagctgc acagatctat gaagcagcta
    22921 cacaaatcgc taaattttct acaccggaga tccgtgtctc gaattttgtc ggcggtacag
    22981 acaaacagcg tcaattaaat cgtctgcatc accaacagcc acaggtcgta gtaggcacgc
    23041 ctggtagaat attagacatg atgaatgaac aagcgctgaa gattcacacc gcatttgcat
    23101 ttgttgtcga tgaagcggat atgacattgg atatgggttt tttaaatgaa gtagaccaaa
    23161 ttgcaagtcg attaccagaa aagctgcagt ttttagtttt ttctgccact attccagaaa
    23221 aacttcgtcc attcttacga aaatacatgg aaaatccaat cattgaagaa atcaaaccaa
    23281 aagcagttat ttctgaagac attgaaaatt ggctgatttc aacaaaaggt aaagatgaga
    23341 atcaaatgat ttatcaactg ctgacaattg gtcatcctta tttagcaatc gtatttgcca
    23401 atacaaaaca gcatgtggat gaaattgccg attacttgaa aaaccaaggc ttgaaagtag
    23461 caaagattca tggagacatc accccaagag agagaaaacg agtgatgcga caagtacaga
    23521 atctggagta tcaatatgtt gtagcgactg atttagctgc tagagggatc gatatcgaag
    23581 gtgtttctca tgttatcaat gcagaaattc cagaagatct agacttcttt atccatcgtg
    23641 tcggacgtac tggacgtaat ggcttaaaag gaacggccat tacgttatat tcgccaaatg
    23701 atgagcaagc cattgatgcg atagaaaaat tgggcgtctc atttgaacca aaagaaatca
    23761 aaaacgggga aatcgtcaaa acatacgatc gtaatagacg tgccaaacga gaaaaatcaa
    23821 aagatgtatt agatcccacg ctgattggtc ttgtgaagaa gaaaaagaaa aagatcaagc
    23881 ctggttataa gaaaaaaatc gattgggcaa ttgcagaaaa aaataaaaaa gaacgcaaaa
    23941 tcgaacgcag acagcaaagc cgtactgctc gtaaaaataa gaaaaattca cataaataaa
    24001 aaacaagagg ttgtgacaaa aggcgtgtca caatctcttg tttttaagct agactttttt
    24061 ccattccgat acaaagtact tcatcttcaa taaaaggttc tgtgcaagta tgataccctt
    24121 gtttttcata aaaatctaca gccgtcattt cagctactac ataactagtc tgacaacctt
    24181 ctgaaaccgc ccgtttttca gcagcttcaa gcaactgacc gcctaaccct tttcctcggt
    24241 attcttttgc tacgcaaaat cgttccggct tgaagcaagt aggtgttttc ttttcataac
    24301 gcaaagtagc tactggtctt ttttgatcaa gaaatagata ataggtttgt tgcgggatat
    24361 cttttccgtc gaattctagt tctgcaggaa tcccttgttc taatacaaaa acttgatatc
    24421 tcaaaaagca gctagctgcg cgtatccatt catccgctcc aactattttt ttcatcatat
    24481 tcctccttgt catgaatagc agagatgtat tgattccttt attcttatcg taccaaaaat
    24541 cgtggagtat gaccaaaagg aaatgaaaat aaaaattgag atattgacag tgatcaatag
    24601 aatgcgtata ctaattaagt acgaaaagga tatgatttgt tttttaagac tcacagaaaa
    24661 ttctttcttg gctgaaaaaa gaatagtcac taatcaaatt gctccctttt tctattttgg
    24721 tagaaatacc gacgcagctc agcgttatcg agtttaagag gtaatgatta cacatcattg
    24781 caatcaaggt ggtaccgcga gttttcgtcc ttggctgtga tggtgttttt ttgtatttaa
    24841 tttatagaaa gcaggtaatg acatgaaaca attaagtagt gcacaagttc gacaaatgtt
    24901 tcttgatttc ttccaatcta aaggacatac gattgaacct agtgcgtctt tgatccccgt
    24961 gaacgatcca accttattgt ggatcaactc tggtgttgca acattgaaaa aatattttga
    25021 tggttcagtt gtgccagaaa atccaagaat cacaaatgcc caaaaatcta ttcgaacaaa
    25081 tgatatcgaa aatgtcggta aaacagcacg gcaccatacg atgtttgaga tgctgggtaa
    25141 tttttcgatt ggtgactatt tcaaaaatga agccatccac tgggcatggg aatttttgac
    25201 tgatccaaaa tggatggcat tcgatccaga aaaactttat gtaacagttt atccaaaaga
    25261 tacagaagca aaacgcatct ggcatgaaga agttggcctg ccactagatc acattattga
    25321 tgtagaagac aacttctggg atatcggtgc aggcccatgt ggtccggata cggaaatttt
    25381 ctatgaccgt ggagaagctt ataacgattt agcagaagac gatcctgaaa actatccagg
    25441 aggagaaaat gaacgttatt tagaaatctg gaacttagta ttttcagaat tcaaccataa
    25501 gcctgataac acatttgaac cacttcctca taaaaacatt gatacgggga tgggattgga
    25561 acgcatggtc tcaatcgttc aagatgcgcc aacgaacttt gagacagact tgtttatgcc
    25621 tattatccac gccgtagaaa agctaagcaa acaagtaaaa tatggtgaaa acccagtaac
    25681 agatatttct tacaaagtaa tcgcagatca tattcgcgcc ctttcatttg ctattggcga
    25741 cggtgcattg ccatcgaatg aagggagagg gtatgtctta cgccgtttgc ttcgtcgtgc
    25801 tgtcatgcat gggaaaaaat taggtattga acaagcattc ttatatcgtt tagtccctgt
    25861 tgttggggaa atcatggtaa gttattatcc tgaagtactt caacaaaaag aatttattga
    25921 aaaagttatc cgtacagaag aagaacgttt caatgaaaca atcaatgaag gtttggaaat
    25981 cttaaatcaa gtgatccaag aagtaaaaaa cgaaaacggg acgactctag caggaaaaga
    26041 catcttcaaa ctatacgaca catatggctt cccggtagaa ttaacggaag aggtggcaga
    26101 agatgaagga ctgaaagttg atcatgctgg ttttgaaaaa gaaatggatg cacaacgcga
    26161 acgtgctcgc tctgcccgca gtactgaaaa atcaatgggg gtacaatctg ctttattgac
    26221 agatatcaaa gtagaaagta aattcattgg atacacagaa caagaatcag atagtcaatt
    26281 attggtcatc gttaaaggtg agtctctttt gaatgaagtc gcagaaggtg aagctcaaat
    26341 gatctttgat caaacacctt tctatgcaga aatgggtggg caagtagcgg atcaaggatg
    26401 gatcatgaat gaagtaggcg aaatcgtagc agttgttaca gatgttcaaa aagcgcctaa
    26461 tggtcaattt atgcacaccg tagaagtaaa agcaccaatg agtgaaggac aaacctatca
    26521 tctattgatt gatcaatcga tgcgcaatcg gatcatcaaa aaccatacag caacccatct
    26581 actgcacaag gcattgaaag aagtacttgg cgagcatgca aatcaagctg gttctttagt
    26641 cgctcctgga catttacgat tcgactttac ccactttggt caaatcactt cagatgaatt
    26701 agctgaaatg gaaagaatcg tcaacgaaaa aatctgggaa gctatcccag ttgaaacaat
    26761 tgaaacagat atcgatacag ccaaaaacat gggtgcaatg gctttgtttg gtgaaaaata
    26821 cggacatgac gttcgagtag taaatattgg cggctggtca atcgaactgt gcggaggcac
    26881 acatgtagca aatacagaaa acatcggaat cttcaaaatc gtttctgaat caggaattgg
    26941 tgcaggcgtg cgacgaatcg aggctgtaac aagtaaagaa gcgtacgaac tacttgaaga
    27001 agaagaaaaa caactaaaac aagtagcaac aattgttaaa tcacctcaat tgaaggaagt
    27061 tgtttcaaaa gcggaacaat tgcaacagca agtaagagag ctacaaaagg aaaatgaaca
    27121 attagctagc aagttagcga atcaacaagc tggtgatgta ttcaaagatc cagaaacgat
    27181 caatggaaaa acaatcattg cagcacaagt aaaagtgaaa gatatgaatc agctgcgtca
    27241 attagctgac caatggaaac aaaaaaatat ttcagatatt cttgttttag caaacgaaca
    27301 agaaggaaaa gtcagtctgc tagcggcaat gacgaaagaa tcaaatgaag ctggactaaa
    27361 agctggcgac ttgattaaag caatcgcacc gaaagtaggc ggtggcggtg gtggacgtcc
    27421 agacatggca caagctggcg ggaaaaatcc tgctggtatt ccagatgcac ttaaagctgc
    27481 gaaagaatgg gttgcgcaac aatagggcgt ttacgtttga cagaaaaaca aaagttcaaa
    27541 cattgaacga aacatgatta tgggggcgaa gacaaacaag tcttgccctc atactttttt
    27601 caattggctg gcgcatctaa tcaaatggtc ggattcggat ggtctttagt tgacgaaagt
    27661 gacagaaatt tttacgatta gtataaataa aaaggggtga aaaaatgaat agtagaaata
    27721 agacctttcg actagtctta cgtgcgatct tgttagcaat catcatcgta caagcgatgg
    27781 ttccttggct ggggtttatt ccattaggat tcattagttt aacaattatc catatcacag
    27841 taattattgc tgcagtagta ttaggcccaa aagatggcat ggtgattggc ttgttttggg
    27901 gaatcgctac aattgttcgt gcctatgcca tgccaacaac gccatttgat acattagtgt
    27961 tcacgaatcc gattatctcg gtagttccca gagtacttgt tggcttagta gcaggcttgg
    28021 ttttccactg gatttatcaa aaaaataaat caataacgat cagctcagcc tttgcagggg
    28081 tactgggttc attggtcaat acagtcttag tgttaggatt catgggatta ttttatacag
    28141 gtgcaacggc agatgcctat ggtgtagatt ctgctctttt atttaaaacc ttagcagggg
    28201 ttgcagcaat caatgggata ccagaagcaa taggtgcggg aatcataaca ccattactag
    28261 caaaagcgtt atttgcagca acaccgttga aaccagaata atgaccatct tttaatagta
    28321 ataggacgca cgatagatag aaaaaggaag agaggagagg aaaacaatcg tcctctccat
    28381 ctttttacct gagcaacaag caaactagaa ggggtaaaaa acttggctaa gaaacaagaa
    28441 gaaaaaaaga aacaaatacc gcaacagcgg acaaaaaaag gagtactagc gggtatctta
    28501 ggattggtta cgaatctgat cttgtttgtc tctaagctaa tgatcgggct tttttcagga
    28561 agtgtctcca ttatggctga tgcagtgaac agtctatcag atactgcttc atccatattg
    28621 actttgatcg gatttaaaat tgctgcaaag ccagcagatc aggaacatcc ttatggacac
    28681 gaacgcttcg aatatatcag tgggttattt gtttcgatca tcattactta tgtaggattt
    28741 caatttttag agtcttctgc tgaaaaaatc atccatccag aaaatgtctc tttaacaccc
    28801 atcgtctttt ttgtcctaat catttccatc tttttgaaat ttgcacaagg caaaatgtat
    28861 caggttattg cgcaatccat tgattctgaa acattgagag ctacagcaac agatagttat
    28921 aatgatgtct ttactacact ggctgtattg ctttcagcag caatcgaatg ggtaactgga
    28981 tggcgtatcg atggatatat aggttttgct ttagcattat atatccttta tagtggattg
    29041 acgatgcttc gaaattttgt atacgaacta ttaggaagtc gcccaacgcc agcagagatt
    29101 gaagaaatgg aagagcattt aaatgcttgc gatgatatac ttggctatca tgatttattg
    29161 gtgcataact atggacccaa taaacgcttt gcatcagtcc acatcgaagt agatgaatca
    29221 atgaatttga atgtggctca taagattatt gatctgatag aaaaagactt taaaaagtct
    29281 ttggatgtgg aacttgtctg tcacttggat ccaatgcctg ttgataacga aaaatactat
    29341 aatatcttgc ggcagttaaa gcaaattctt cttgaaacag gtacagggct taatattcat
    29401 gattttagag taattcatca aggaaaaacg ctgcaatttg atgtagtggt ccctgagaaa
    29461 ttccagtttg atgatacaga actagaaaga atcttacgta gtcaaataga agaaaaaatc
    29521 ggagattacc aattggaaat tacgtttgat cacaattatc ttttatatta aatgagaaac
    29581 tataaaaaga ctccttaaag tttttaattt aaagagtctt tttttctttt aattgtttat
    29641 ggagagcctg acgtgcaaga ttatctgctc ctttattttt gttttcgggc aaccattgca
    29701 ggatcaataa agaaaattcg gatagaagtt cttggattct ttgaaggtag ggaaggaaga
    29761 gctcgttctt cgttgcgttt ttgtcgaccg tagaaactag tgttttgctg tctgaaaaac
    29821 agaaaatcgt ttcattctgt aagtcgtttt tctttagaaa ttctaaagtt tttaaaaaga
    29881 cttcgaactc tgcttgatgg tttgtcccta tagaaagcgg gatagatagc tgttcttgtt
    29941 taccttgata gacaatcaag attccgcctc cactaggacc agggttgcct tttgtagagg
    30001 catcaatgta tacttttaac ataaaaagag attctccttt ttgttcaaac taatgttatg
    30061 gtaagattat atcattactg aatcggtgag aggttagaga atgaaaaaaa atattcgatg
    30121 gcagcctgaa ctggcacata cgatcatcta ttggtcattg accttgatca ttctgttttt
    30181 tagtctgatt ctttctttag aaaacacagg tccttattgg aaaagcaatt ttgttttagg
    30241 tatttttctt gtttttctat ttctaggttt gcgacgttct tttattttaa aatcaaactc
    30301 tgtgattata cgttatgctg ctttttggaa agaccgagaa atatctgtcg gacagattcg
    30361 atctttgacg ccaactaata ctggggtgca aatgatgttg caagatgaga aagccccatc
    30421 tgttttcatg atgcgaaaaa aagatcaagt agtccttttt gatcattttg aaaaaaacgg
    30481 acccaaatca gaatcaattc cttatcctta tacgaaaaaa agaaaaagct aattttaaaa
    30541 atcagaacct aaaaaaacga atttttacag gaaaagaaaa gagtgagacg tacggttcat
    30601 cccgtttagc gtctcactct atttgtttat ctattcatgc atactagtat cggtagtctg
    30661 aacgttgcct ttcttgacat gggcagcttg tgttcctcga ttcccttcga tgatctcaaa
    30721 ttcgaccact tgattttctt ccaaagtttt gaatccctct tcttcaatcg cagtaaaatg
    30781 tacaaaaatt tcttctgtgt catcataact gataaagccg tatccttttt tgttgtcaaa
    30841 ccattttaca attcctgtgg tcaaagcact cacgaccttt ctaaagtctg atcatagcct
    30901 tcattataaa gagtatttat aagtacgtca aataaattaa aaacctgtaa ttttatgttt
    30961 tttttattat aaaaaaaggt ataattggca tatcttaaca aagaagatat tttgttgaat
    31021 aaagttcgtg aaaaggggta atcaaatgaa gaatgatctg caaatttcac aagaaacaga
    31081 gctagaaccg attgttgaag tggctagtaa attagatctt aaggaagacg atttggaact
    31141 gtatgggaaa tataaggcga aaatcaattt ttctggaatc aatcgatcaa aggataacgc
    31201 tgaggggcat ttgatcttag ttacttccat caatccaaca ccagctggcg aaggaaagtc
    31261 aacgatcact gtagggttag gagacgcgct caataaaatc aataaaaaat ctgttattgc
    31321 attgcgagaa ccctcacttg gtcctgtgat gggaatcaaa ggtggtgctg caggcggagg
    31381 atatgcacag gtattgccta tggaagatat caacttgcat tttactggtg acatgcatgc
    31441 tatcacaaca gctaataatg cattatctgc tcttttagat aatcatattc atcaaggaaa
    31501 tgaattgaac atcgatgccc gccgagtgat ttggaaacgg gtagtcgatc tcaatgatcg
    31561 tgaactgcgt aaagtaattg ttggcttggg tgggccaatc caaggtgttc caagagaaga
    31621 tgggtttgat atcacagttg ccagcgaaat catggctatc ttgtgtcttg cggcagacct
    31681 tgaagattta aaggctcgtc ttgcaaggat cgttgtcggc tatacatttg accgtcagcc
    31741 tgtgactgct ggagacttga aagcagaagg cgctctggca ttactgctga aagatgcaat
    31801 caagccgaat cttgtacaaa caatttacgg aacaccagct tttgtccacg ggggaccgtt
    31861 tgccaatatc gctcatggct gcaatagtat tttagctacc aagacagcat tgagattagc
    31921 agattacgtt gtcacagagg caggatttgg tgcagatctt ggcggagaga aatttttaga
    31981 tatcaaagta cctaacttga aaaaagcgcc ggatgctgtg gtgattgtcg ccactgtccg
    32041 tgcattgaaa atgcatggcg gaatgaaaaa agatgaactt aaaaatgaga atcttgatgc
    32101 tttgaaagta ggttttgcaa atttaaagcg ccatatacgt aatatggaac aatatcagct
    32161 acctgtgatc gtcgcaatca atgaattcgt aacagataca gacaatgaac tcacactgtt
    32221 ggagcacttg tgtgaaaatc aggggattat tgccaagaga gcgagtgtat gggcagatgg
    32281 agcagaaggc ggcgttgatt tagcaaaagc tgtagtacga ttgattgata gaaaagaagc
    32341 taattacaag ccattatacc gcctagaaga aacgatacaa gaaaagacag aaacaatcgt
    32401 caagaaaatt tacggaggaa atggtgttgt tttcagcaaa aaagcgcaaa aacaaataga
    32461 agagtttacg aagaatgaat gggacaaact gccgatttgt atggcaaaaa cacagtattc
    32521 attttctgat gatccttcac tgctaggtgc accggaagat ttcaccatca cgatccgcga
    32581 aatcatccca aaattagggg caggattttt agtagccttg actggagatg ttatgacgat
    32641 gcctggtttg cctaaaaaac ctgctgcatt gaatatggac gtgacaaatg acggagaagt
    32701 gttgggattg ttttaagctt gattataatc taccgtatac taaataactt tcattaaaca
    32761 tcgaaggagc tgtattttga ctacagctt
//
LOCUS       DACECY010000030        31295 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.031, whole genome shotgun sequence.
ACCESSION   DACECY010000030 DACECY010000000
VERSION     DACECY010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.031"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(135..512)
                     /locus_tag="IWO38_001469"
     CDS             complement(135..512)
                     /locus_tag="IWO38_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313563.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            768..1496
                     /locus_tag="IWO38_001470"
     CDS             768..1496
                     /locus_tag="IWO38_001470"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ0313564.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1496..1750
                     /gene="purS"
                     /locus_tag="IWO38_001471"
     CDS             1496..1750
                     /gene="purS"
                     /locus_tag="IWO38_001471"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ0313565.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1752..2423
                     /gene="purQ"
                     /locus_tag="IWO38_001472"
     CDS             1752..2423
                     /gene="purQ"
                     /locus_tag="IWO38_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ0313566.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2424..4646
                     /gene="purL"
                     /locus_tag="IWO38_001473"
     CDS             2424..4646
                     /gene="purL"
                     /locus_tag="IWO38_001473"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ0313567.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4631..6070
                     /locus_tag="IWO38_001474"
     CDS             4631..6070
                     /locus_tag="IWO38_001474"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ0313568.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6102..7145
                     /locus_tag="IWO38_001475"
     CDS             6102..7145
                     /locus_tag="IWO38_001475"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ0313569.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            7142..7720
                     /gene="purN"
                     /locus_tag="IWO38_001476"
     CDS             7142..7720
                     /gene="purN"
                     /locus_tag="IWO38_001476"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ0313570.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7717..9252
                     /gene="purH"
                     /locus_tag="IWO38_001477"
     CDS             7717..9252
                     /gene="purH"
                     /locus_tag="IWO38_001477"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ0313571.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9268..10542
                     /gene="purD"
                     /locus_tag="IWO38_001478"
     CDS             9268..10542
                     /gene="purD"
                     /locus_tag="IWO38_001478"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ0313572.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10664..11578
                     /locus_tag="IWO38_001479"
     CDS             10664..11578
                     /locus_tag="IWO38_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ0313573.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11711..13276)
                     /locus_tag="IWO38_001480"
     CDS             complement(11711..13276)
                     /locus_tag="IWO38_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0313574.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13269..14720)
                     /locus_tag="IWO38_001481"
     CDS             complement(13269..14720)
                     /locus_tag="IWO38_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0313575.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14734..15750)
                     /locus_tag="IWO38_001482"
     CDS             complement(14734..15750)
                     /locus_tag="IWO38_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0313576.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15970..17058
                     /locus_tag="IWO38_001483"
     CDS             15970..17058
                     /locus_tag="IWO38_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0313577.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17112..18638
                     /locus_tag="IWO38_001484"
     CDS             17112..18638
                     /locus_tag="IWO38_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ0313578.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18640..19845
                     /locus_tag="IWO38_001485"
     CDS             18640..19845
                     /locus_tag="IWO38_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0313579.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20102..21079
                     /gene="guaC"
                     /locus_tag="IWO38_001486"
     CDS             20102..21079
                     /gene="guaC"
                     /locus_tag="IWO38_001486"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ0313580.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21343..22767
                     /locus_tag="IWO38_001487"
     CDS             21343..22767
                     /locus_tag="IWO38_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ0313581.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23228..24337
                     /gene="pdhA"
                     /locus_tag="IWO38_001488"
     CDS             23228..24337
                     /gene="pdhA"
                     /locus_tag="IWO38_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ0313582.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24340..25317
                     /locus_tag="IWO38_001489"
     CDS             24340..25317
                     /locus_tag="IWO38_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ0313583.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25342..26637
                     /locus_tag="IWO38_001490"
     CDS             25342..26637
                     /locus_tag="IWO38_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HAQ0313584.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26645..28051
                     /gene="lpdA"
                     /locus_tag="IWO38_001491"
     CDS             26645..28051
                     /gene="lpdA"
                     /locus_tag="IWO38_001491"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ0313585.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28213..28488
                     /locus_tag="IWO38_001492"
     CDS             28213..28488
                     /locus_tag="IWO38_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ0313586.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28494..29261
                     /locus_tag="IWO38_001493"
     CDS             28494..29261
                     /locus_tag="IWO38_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ0313587.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29429..31258
                     /gene="typA"
                     /locus_tag="IWO38_001494"
     CDS             29429..31258
                     /gene="typA"
                     /locus_tag="IWO38_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ0313588.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
BASE COUNT        10236 a         5368 c         7030 g         8661 t
ORIGIN      
        1 aaccaaaaaa gaagcagatc cgttagcggt tctgcttctt ttagtttatt atattttctt
       61 atcaataata ggaacctgat tttaaaagcg gctacctctt taccgtgcct ctttacatac
      121 aaaaaataga ggaattaagc gtaaatggct tggctattat aactaaaatg ctggttatgg
      181 agatgttgca gcaagttttc gagaaaagcg tattccatct cttgtccatt gaataagatc
      241 tggatacgat aatgattttt gaaacgcttc tgttcatgaa tctgactgat tgaaagttcg
      301 cctttgtgtt ctgtctgata aagaaaagtc cttttacggc attcagcaca gcgcttggtt
      361 gctcggcgga tttttcggaa tgtataaaaa ctagtatctt tattgcataa ataaacgtct
      421 ttgatcgaca gcaaatcatt atatagacag gaacgttctt cagctgttaa ttctgataaa
      481 aaaagctgac cttgttttat tgcatcttcc atcggcaagt gcctcctcga acccattatc
      541 caaattttta gttaattcta atataccatc attaagtata aatgtaaacg cttgttgatg
      601 gtataagatt ttcgtttctg ataaaacacg aacattaaaa gaatctaatg ccttaaatgt
      661 acatattttt gttgatttat aaaaagatga ttgctaaaat ttaaagtgaa aaaacgaaca
      721 aaaaacgatg tatttgcttt taatgtaaat aaaaaggaga gacgtacatg gagaagaaag
      781 aacttttgta tgaaggaaaa gccaaaaaaa tatatcttac ttctgatgag aacttgattt
      841 ggatagaata tttagatcaa gcaactgcgt tgaatggcgc gaggaaagac cagataagtg
      901 gaaaaggaga attgaataat caaatcacaa gtgcaatttt ccgttatctg actcaaagag
      961 gaataaaaaa tcattttatt gaacaattgt ctaaaaccga acaattagca aaaaaagtac
     1021 aaattatccc tctggaagtg gttatccgaa atattgcagc aggaagtttc tcaaaacgtc
     1081 taggtgttcc cgaaggtaaa gaattcatca aaccaatcat tgaattctac tataaggatg
     1141 accagttgga tgatccattt atcaatgaag agcatatcta tttcttacaa cttgcaaagc
     1201 cggaagaaat cagtgagctg aaagcccaag cgttggctat taatgaaatt ttgaccgaat
     1261 tatttgctaa ggtcggtttg gttttagtag atttcaagct agaatttggc cggtcgaatg
     1321 gacaaatttt gcttgcggat gaagtaacac cggatacttg ccgattatgg gatgaacgta
     1381 cgaatgaaca tatggacaaa gacgtttacc gcagagatct aggtgatttg atcccagtct
     1441 atcaagaagt atataaccgt ctatcggaac taactgaaga aaaggagcga gtatgatgta
     1501 tttcgtaaaa gtatatgtaa cctataaaga ttcagtgtta gatcctcaag gggaggcagt
     1561 aaaaaaagca gtccatcgta tgggatatga aacaatcgaa gatatccgaa taggaaaata
     1621 ctttgagata caacttgctg atgcagaaga accaatcgat acagtgattg aaacgatttg
     1681 tgacaagctg cttgcaaatg taaatatgga aacataccga tatgaaatca aaaaaatgga
     1741 ggaagtataa catgaaattt gcggtcatcg tttttccagg ttcaaattgt gacgcagata
     1801 tgttatgggc agttcgcgag atcatgggcg ctgatgccga atttgtacgg catgatgagt
     1861 cctctttaga aggatttgat ggggttttac tgccgggagg attctcctat ggtgactatt
     1921 tgagatgcgg tgcgattgct cggttttcta caatcatgga agaagtgatt cgttttgctg
     1981 aagaagggaa accagtattc ggtacatgca atggattcca gatattgaca gaagcaggac
     2041 ttttgccagg tgtactgaga cgaaatgagt ctttgcattt tatttgcaaa actgtaccat
     2101 tgaaagtcaa ccccaagacg gcctttactt cagagtatga agccgacgaa ataatctatc
     2161 ttccagttgc tcatggggaa ggaaattatt tctgtgacga agaaacactt caagaactaa
     2221 aaaaaaataa tcagattgcc tttacttata atgacaacat caacggcagc gtggaaaaca
     2281 ttgcagggat tacgaacaaa gcagggaatg tgttaggaat gatgcctcat ccagaacgtg
     2341 cagtagaaga cttgctagga tcagaagatg ggaaacggtt ctttgcttcc attttaaaaa
     2401 acttcggaaa ggtgaccgta tagatgaaaa cattgaaaga accgacagct gaagagatca
     2461 agaaaaaaag aatctatgcc gaatggggat tgactgatga agaatatcga atgatcgaag
     2521 aagatatctt aggaagaatg cctaattata cggaaactgg actgttttct gttatgtgga
     2581 gcgaacattg ttcctataaa aattctaaac cggttctacg caaatttcct actagtggac
     2641 ctcatgtgtt gcaaggccct ggagaaggcg caggcatcgt cgacatcggt gatggactag
     2701 cagttgtctt caaagcagaa agtcacaatc atccttcagc cgtggagcct tatgaaggag
     2761 ctgcgacagg agttggcgga atcatcagag atattttcag catgggggct cgtccaattg
     2821 cgttgctaga ttcattgagg ttcggcgaat tagataatcc aagaacgaaa tatttactag
     2881 aagaagtagt tgcaggtatc ggcggatacg gcaattgtat cggcataccg actgtaggcg
     2941 gcgaaattgc atttgatcct tgttatgaag gaaacccatt agtcaatgca atgtgcgtag
     3001 gtttgattga acacaaacat atccagaaag ggcaagccgc tggcgtcggt aattcgatca
     3061 tgtatgtagg tgcaaaaaca ggtcgcgacg gtatccacgg agcaactttt gcctcagaag
     3121 aatttaatca agaagaagaa cagcaacgtt ctgctgtaca agtaggcgat ccttttatgg
     3181 aaaagctgct gcttgaagcg tgtttggaat taatcttgca tcattcggat tctctaatag
     3241 gtatacagga tatgggagca gcaggacttg tttcatctag tgcggaaatg gcttcaaaag
     3301 ctggctcagg acttatattg acattagatg aagtcccaca aagagaaact gggatgacac
     3361 cttatgaaat gatgttatcg gaatcacaag aacggatgtt gatttgtgtt aaatcaggag
     3421 aggaagaaaa aatccaagaa ctatttcaga agtatgatct agatgcagtc actatcggta
     3481 aagtaacaga cgacgggcaa tatcggctat atcatcaagg agaagaagta gcaaatctgc
     3541 cagtcgatgc attagctgaa gatgcaccag tttatcataa agaaatgaaa gaaccagctc
     3601 gaatcgtaga atttcagcag ttagctcctt atcagccagt tatcgaagaa cctggacaag
     3661 tattgcttga tctgctgcag caaccgacga ttgcttcaaa acgttcaatc tacgaaactt
     3721 atgattcaca agtacaaacc aatacagttg tccgtccagg aagcgatgct gctgtgatga
     3781 gggttagagg gacgaataaa gcgttagcta tgaccacaga ctgcaacgcg cgctatctgt
     3841 atttggatcc aaaaatcgga ggacaaatag cagtggcaga agctgctaga aatattattg
     3901 ctagcggtgg taagcccttg gcgatcaccg actgcctgaa ctatggttct cctgataaac
     3961 cagaagtttt ttgggaatta tcgacttcag ctgacggaat tgctgctgcg tgtgaaacgt
     4021 taggaactcc tgttatttca ggaaatgtct ctttatataa tgaaacggat ggtcaagcaa
     4081 tctatccaac accaatgatt ggtatggtcg gattgattga ggatcataaa catatcacta
     4141 ctcaggaatt caaaaaatca ggggatctaa tctatatatt aggtaagacg tttgcagatt
     4201 ttgatggcag tgaattacaa aagatgcagc ttggaagaat cgaaggtgtg atccgaaact
     4261 ttgatctatc cattgaaaag agaaaccaag agttagtatt gacagccatc caaaacggat
     4321 tgattgaaag tgctcatgac tgttcagaag gtggcttggc aattgcactg gcagaatcag
     4381 ctttcaaaca tcagctaggg atatctgtac agtttgaatt gtcctctgct caattatttg
     4441 cagaaactca atcaagattt gtgttgacag tcgcaccgga aaataagaca cgattcgaag
     4501 aaatgatggg agatgctgct gttttagcag gtaaggtgac ggatgaagcg ataatcgaaa
     4561 tttcagcaac tgacggtcag atcaaaatag aaactgcagt agcaagaaag tgctgggagg
     4621 atgcaatcgt atgtcttatg aagtgaagag tttaaatgaa gaatgcggga tcttcggtgt
     4681 ttggggtcat ccagacgccg ctagtgtggc atacttcggg ctccatagtc ttcagcatcg
     4741 aggacaagaa ggtgcaggta tcgtgtccaa tgatcgagga aagcttaagg ggcatcgaga
     4801 tttggggctg ctttctgagg tattcaaaga tcaaagaaaa ttgcagcagt taacaggaga
     4861 agctgcaatc ggacatgtac gttatgcaac agcaggcaat ggaagtgtgg acaatattca
     4921 gccttttcta ttcaaatttc atgatcagtc gatcggctta gcgcataatg gtaacttgat
     4981 caatgctcgt tcccttagaa aagagttaga ggaaaatggg gcgatcttcc attctaactc
     5041 cgatacggag atattgatgc atttgatccg acgaagtgaa gaaacagatt ttttaaataa
     5101 attaaaaagt gctttgaatg aagtcaaagg tggctttgcc tatttattaa tgacagaaac
     5161 ggcaatgatt gccgctttgg acccaaatgg ttttcgtcca ttagcaatcg gacaaatgaa
     5221 aaacggagca tacgtgattg cctcggaaac ttgtgcatta gaagttattg gggcacgttt
     5281 tataagagat gtccagcctg gtgagatagt gatcatcaat gacgaaggaa tccagattga
     5341 ttgctttacg aaggacacgc agctttctat ttgttcgatg gaatatatct attttgccag
     5401 accagattca aacatcgcag ggatcaatgt gcatactgca cggaaaaata tgggacggaa
     5461 tttagcgaaa gaatcgccag taaaagcaga tatggtcgtt ggcgtaccta attcttcttt
     5521 gtcagccgca agcggttatg cggaagctag tggaatccct tatgaaatcg gattagtaaa
     5581 aaatcaatac attgcccgaa catttatcca gccaacgcct gagctaagag agcaaggtgt
     5641 tcgaatgaaa ttatctgctg tacgaggagt agtggaaggt aaaaaggtga ttatggtcga
     5701 tgattcaatt gttcggggta cgacaagcag acggatcgtc catcttttga aagaagcagg
     5761 ggcaaaagaa gtccatgtga ggattgcttc tcctcctttg aaatatccat gtttttacgg
     5821 gattgatatt caaacgcgta aagaactcat tgcggcaaat cacacgattg atgaaatcaa
     5881 agaatgtatc ggtgcagatt ctttaggttt tttaagtgaa gagggtctga tcgaatcgat
     5941 cggtctggaa aaagatgctc cgtattcagg attgtgtatg gcttatttca acggagatta
     6001 tccgacgccg ttatatgact atgaagaaaa atatcaggaa tcactaaaag aaaaagtatc
     6061 gttcttttaa aagcagacca agaagggagt atatgaattc aatggcaaat gcatacgcaa
     6121 aagctggcgt agatgtcgaa gctggatatg aagtagtaga acggatcaag aagcatgtca
     6181 aaaaaacgga acgtttaggt atgatgggcg ctataggagg attcggcggt tgttttgatt
     6241 tatcggtctt tcaattaaaa gagccagtcc ttgtctcggg gaccgatggc gtggggacga
     6301 aattagcagt ggcaatcgaa gcagacaaac acgatacaat cgggatcgac tgcgtagcga
     6361 tgtgtgtcaa tgatatctta gcacaaggcg cagagccgtt atatttctta gactacttag
     6421 caacggggaa aaatgatccg ttacgtttgg aaaatgtagt aaaaggtgta gcagatggat
     6481 gtgttcaagg aaatatggcg ctgattggcg gagaaacagc tgaaatgcct ggtatgtact
     6541 caggtaatga ttatgatcta gccggatttg ctgtagggat agctgaaaaa tctcaattat
     6601 tgacaggaga aacgatcaac ccaggagatg tgttgcttgg aatagcatct agcgggattc
     6661 attcaaatgg gtactctctt gttcgcaaaa ttttctttga gcagcagcag ttttctttat
     6721 catcaaaact gccagaattg aaaaaaacac ttggcgaaga attgcttgag ccaacgaaaa
     6781 tctatgtttc ttccttgctt ccgctgatca aagaaaagaa aataaaagga gctgcacata
     6841 tcactggtgg agggtttgta gaaaatatcc cgcgaatgtt accagaatct atggctgcac
     6901 aaatcaaact tggctcatgg ccgattcttc caatattcgg ctgtttacaa aaatatggtg
     6961 aaattcctga gatggagatg tatgaaatct tcaatatggg catcggtatg gtcctggcag
     7021 tcgaagaatc gaaagtaaaa gaaatagtac acgaaattga gcaagctggc gaaaaagttt
     7081 atgaaatcgg gaaagtgatc gccaaagaaa aacatcgtgt ggtgtttata ggagaagaac
     7141 gatgagaatc gctgtttttg cttctggaaa cggaagcaac tttcaagcgc tagccgatta
     7201 tctatcgaaa aaaggattgg aatcttcgat tgactggttg ttttgtgatc agcctgaagc
     7261 atatgttttg aaacgtgcaa ctgcgttgag tgtaccggca gattgttttt cacctaaaga
     7321 atttgattca aaaaaagagt atgaagaagc aattttgcat aaattgaaag aaaaaaagat
     7381 cgatttgatc gtattagctg gatatatgcg aatcattgga ccggtattgc tgaaaaatta
     7441 tgataaaaga atcatcaata tccatccgtc tcttttacct gccttccctg gtttgcatgg
     7501 catacgtgat gcctttgaag ctggagtaaa agaaacgggt gtcacaattc attatatcga
     7561 ccaaggtgta gatactggac caatcatccg gcaggaaaaa gtgagaattg aacaggagga
     7621 cacgttcgat tctttagaag aaaaaatcca tagagtagag catcgaatct atccagaagt
     7681 gattagtgaa attattgaaa atggaggcta tctgaaatga caagagcatt gatcagtgta
     7741 tcagataaaa ctgggattgt agcattcgcc caaggtttga ttgaacaagg agtagaaatc
     7801 atttcaacag gaggaacaaa gcatgcgttg gataaagctg ggatcaaaac gctttcgatc
     7861 gaagaagtga cagggtttcc tgaaatgatg gatggccgtg tcaaaacact tcatcctaaa
     7921 atccatggtg gtcttttagg aagacgcgat ctccctagcc atatggaggc aatggaaacg
     7981 gaaaatatcc aaccaatcga ctttgtttgt gtcaatttat atcctttcaa agaaacgatt
     8041 atgaaaccag atgtcgaaat agcagaagct attgaaaaca ttgatatcgg tggtcctagt
     8101 atgctccgta gtgcagcaaa aaaccatgaa tttgtcacct ctgttgttga tccatctgac
     8161 tatgaagaag tactttcaga attacgtcag tatgggcata cttcactttc tttcagacaa
     8221 aaactagcgg caaaagtctt tcgtcatact gcggcatatg atgcactgat cgctcaatac
     8281 ttaacggatt gggtggaaga aaaagaaccg gaaaaattga cacttacgta tgaacgaaaa
     8341 caaacgcttc gttacggtga aaatagccat caagccgctg ccttttatca gtccgctctt
     8401 ctggaaaaat attcgatcgc agcagcaaaa caactccatg ggaaagaact ttcttataat
     8461 aatatccgag atgcagatgc tgcgctacgg atcatgaagg aatatgacga gccaacggta
     8521 gtcgccttga aacacatgaa tccttgtggt attgggacag catctacgat tttagaagcg
     8581 tgggaagcag cgtatgaagc agatcctgtt tcaatctttg gtgggatcat cgttttgaat
     8641 cgagaagtag atctgccaac agcagaacag atgcataagc tgtttctaga aatcatcatt
     8701 gccccttctt atacacaaga agctttggaa ttactgcaaa aaaagaaaaa catccgatta
     8761 ctgacagtag actttgcgca ggaagaaaat caaaatcgag aagaaacggt ctcagtctta
     8821 ggtggacttc ttgtacaaga tcaagatctg gcaatagaaa atgaagaaga gtggaaagtc
     8881 gtgacaaaac gtaagccgac tccagcagaa gcaaaagcat tagcgtttgc ttggagagct
     8941 gtcaaacatg tgaaatctaa tgcgatcgta ctggcaaacg aacgtcagac agtaggaatc
     9001 ggagccgggc agatgaatcg agtcggttca gtaaaaattg cgattgaaca agcgcaagag
     9061 aaaatggaag gtgcagtttt agctagcgat gcttattttc caatggatga ttcggttgaa
     9121 tatgcagcca aacatggtat aaaagcaatc attcagccag gaggaagcat caaagaccaa
     9181 gattctattg atatggcgaa taaatacgat ctagcaatgg tctttactga tgttcggcat
     9241 tttagacatt agagaggagt cggataaatg aacattttgg tgatcggtgc aggtggtcgt
     9301 gaacatgcaa taagtaaaaa acttttagcc agtccgaagg tggatacggt gtattgtgcg
     9361 ccaggaaatc cggggatgac aaaagatggt atcaattgtg tcgctatcag tgagcgagat
     9421 cattcagcgt taatccattt tgttaagaaa gaggctattg attggagtat cgttggtcct
     9481 gaagttcctt tgttaaatgg gatcgtcgat gattttatgg cggaaggatt gaaaatcttc
     9541 ggtccgacaa aagcagcagc tctgatcgaa ggttcgaaaa gttttgctaa acaattaatg
     9601 gatacttatc aaatcccgac agctaaatcc agaagttttg aatgttacga aaaagcagca
     9661 gcgtatatca aagaaaatgg tgcgccaatc gtagtgaaag cggacggctt agcagctggg
     9721 aaaggtgtag tcgtggctac cacagtgaag gaagcgctgg atgcggcaga acaaatgttg
     9781 aaccagcatc gatttgggga tagtagcaaa aaaatcgtcg tagaagagtt tttggcagga
     9841 gaagaattct cccttttggc gtttgtccgt aataccaagg tgtatccgat ggtcatttcg
     9901 caagatcata agcgggcatt tgataacgat aaagggccaa atacaggcgg aatgggtgct
     9961 tattcaccag taccgcaaat ttcaaaaaag atgattgaaa aagctgtgga agaagtttta
    10021 aaaccagcgg ctttgggaat gaaagaacta ggacgctctt tcacgggtgt tttatatgcg
    10081 ggtttgattg ctacgaagga aggaccaaaa gtgatcgaat tcaatgcgcg tttcggtgat
    10141 cctgaaacgc aagtcgttct ttctcggttg aagagtgact ttgcgcaagt aatcgatgat
    10201 ttattagaga atcgtacagt agagttaaag tggcagcaag aaggatacaa tgtaggcgtt
    10261 gtggttgcag ctgccggtta ccccgaagaa tatgagacag gaatggttct accagatttt
    10321 ctggaagaag agctttctgt ttactatgct ggagtttcct caaataacgg caaactagtt
    10381 ggagcaggag gacgtcttta tttagtagaa gcatacggtg agacgatcaa agaagcacag
    10441 aaaaaaattt acaccgcgtt aatggataaa acgacagatg gaacatttta tcgtacagat
    10501 attggagaaa aagctgccaa tagcgaactg atcgtgaagt aaatcagctg cttccattta
    10561 taaaaaatac aacaaacata atcggaagcg ccgattttag tgaagccttt tcataaagag
    10621 tgtggtacac ttttgttgtg tatataaata aggaggagtt cagatgaaaa aaattcaatt
    10681 aggtacttct gatcagaaag cagctgcagt gatcttaggc tgtatgcgta tcaacaatgc
    10741 ggaagatcca gtaaaagtga ttgagacagc ttatgaaaac ggcattgatt tttttgacca
    10801 tgcagatatt tatggaaacg gtgaatgtga agaaatcttt gcagatgctc tagcaaaaac
    10861 atcaatcaaa cgagaagatc tttttattca aacaaaatgt ggaatcgttc caggtgtgat
    10921 gtttgatttt tctaaaaaac atatcattga atctgtagaa aaaagtttga cccgtctgaa
    10981 aatggattat gtggatgctt tacttcttca tcgtccagat acattggtag aaccagagga
    11041 agtagctgaa gcgtttgatg aattggaacg gtcaggaaaa gttcgttatt ttggcgtcag
    11101 caatcaaaaa ccaatgcaaa tcgaattgct gaaaaaatat gttcgtcaac cattagtagc
    11161 taatcagctg caatttggct tgaaacatac tggaatgatc gatcaaggga ttcatgtcaa
    11221 tatgacagat gaagcaagta tcgatcatga cggtagtgta cttgattatt cccgattgca
    11281 tgatatgacg atccaagcat ggtcaccata ccagtatgga ttttttgaag gtgtatttat
    11341 cgggaacgaa aaattccctg aattaaacca aacattagaa gaactagcag ctaaatatca
    11401 aacgacacca actggcttgg cttctgcttg gattttacgc catccagcca atatgcaagt
    11461 gatcgccggt acgatgaacg tagggcgaat caaggaaatc gctcaagctt cagaaattaa
    11521 tatgtctcgg gaagattggt acgaattgta ccgtgctgct ggaaacattt taccttaaag
    11581 attgtcataa gcaggtcaaa agggctataa attaaaaaga cagaataact tgaaagctgt
    11641 tttcagcttc gagatattct gtcttttttt attgcaaatg gaaagagtat cattgtacgg
    11701 atttcgttta ttagttattt ttgtttttaa ggaaaagctg tgtccgatat tcttttggag
    11761 aaatcccttg ttttttctta aataaataac tgaaatagtt agggtcatta taaccaattt
    11821 caaaagcaat atctgaaata cgatagtctg attgctgcaa taattttttt gctaattcga
    11881 ttctttgttc tgtcagataa tcgatgaatg tttggttaaa cgattgagaa aaaatcgtac
    11941 tgaaatgaga gggacttaat gcaacttgtt gagcgaccca agttaaggac atatctggat
    12001 cggtaaaatt ttctttgata aaatccaatg catggttcaa aattggctga tatttgttcc
    12061 ttgaaggctg tgagggcaga gcagttaaga aagaaatgaa catataaact gtcgaacggt
    12121 aaagcgagtc gtcggcgctg attgctaagc gttgactggt ggatgtgcaa tgaaccattt
    12181 cttgcgggat tttcttttct ttcaggcaga catgctggag tagcttagtc aagacaaata
    12241 aacgatataa tcgtgttcga gttggtgtgc cttgtggttt cgtcaagtgt tcgatgtact
    12301 tagatacttc gtttttcgat aatgaagaca aatcaagtga gaggtcaaag taaaagacag
    12361 aatcaactgg aagtttcgct tcttgaatca aatcctcata gctgataatt tgttctttaa
    12421 gggaactggc ggtttcaagt atcatctgcg tgatctcaaa agatgcaggt aattcgctga
    12481 ttctttctgc gacaggacca atggcgacag aaaaattatt tttttgtccc ttttctaatt
    12541 catggaccaa cgtttgggct acctgataac tggtctcgat cacattttct tcaagagaat
    12601 caactattaa aaatttgatg aatcgagagg aaacagcaga gaagatcact tgttgattct
    12661 ccctaaataa aaacttcaaa taatcactga gatgagcgta atcttcaaag tctgtactga
    12721 cttggtttgc tgctaataat acggtcattt ttttccctgc taaagagcga ttgaaagatt
    12781 cagcaagttt caatgtttca ctgatcgtaa gtgagccttt gaacaaaccg ttcaagaaat
    12841 gatttttttt caattcgaaa acaagatcag aagaagtgtg cgtttctttt tgctgcaaat
    12901 ctaaagcagt tactgctttt tctattgctt gtttcaattc gctactttta atcggtttaa
    12961 gcagataatc atctgcctgt acctggattg ctcctttcat ataatcgaaa tcgtcgaatc
    13021 cactaataaa tatgagacga gtccaaggaa gtagtttcct tgcttctttt gaaaaagtca
    13081 acccgtccat gaaaggcatt tggatatcag tcagaatgat gtcgggctgt aagtcgataa
    13141 ttgaagacag tgccatctca ccatctccag cttcgccgac ataagtgatt ggataataag
    13201 aaccaaggac agttatttgt tcccgcaagt tattgcgaat gaggtattcg tcttcgatga
    13261 taaaaacttt atacatgttt gtttcctcca attgttttgg gagatagagg gatcatcagt
    13321 gtgacagttg ttccttctcg gtatttgctg tcaatcgtga taggtttttg atcatttccg
    13381 taataaagaa ggagacgctt gtttacgttg taaagtccgt agccgatact gaaatctgaa
    13441 tcgatccctt tggctaactc tttacgtatt tctgccagtt tttctgcagg gatgccaatc
    13501 ccgttgtcta taactttgaa ttggatcatc tcatcaattt tgcatgtggt aatacgaata
    13561 aatcctttgt gacggacgta cttcgttcca tggtatacag cattttcaat caatggctgc
    13621 aagatcattt ttaaaataga aacaggaaaa agtgtttctg gtatatcgat cttatagtca
    13681 agcatatggc cgtatcttat ttttaagatg actagataat cattgatatg gctaatttct
    13741 ttttcaagag gaatccagtc ttgtcctttg cttaaagacg tacgaaaaaa gccggaaagt
    13801 gcatgggtca tagtcagagc ttcttcaaag cagtcttgtt cgatcaaaga aataatggca
    13861 tccaaactgt tataaataaa atggggagta atctgagctt gcaatacacg aacttcactt
    13921 tgtgctaaat ggtattgttt caaagcgttc tcccgtaaag actggtttag atcttccgcc
    13981 attttattca tgctagcggt aagtgtttgt aattccagtg tttccggcag ggttaaacga
    14041 aagccaaggt tccctgaaga aagttgttct gacatctcga tcagctgatt gatcggttct
    14101 tggacctttc tctctaaaaa gcgacgattc tttttcgcat agaaaaaaat ggtactaaga
    14161 actccaagtt ccactattgt taatagaatg agcgagcgga caagtgtcgt attttttttg
    14221 cttgctaagt cgatctccac acgaacaaaa tcttgtaaga tatctgacag cagttgagtg
    14281 acagaatcta cctgattgat gatctcttca ttggtatcca taaaatcttt tgccaagata
    14341 ttattgatca gctcgttttg ataatcttcc agtgtatcga tcgtacgtag cgatacatct
    14401 aacgtactaa tttctttaag tgtatttgta ttatttttga tctctcgaat atcattccta
    14461 agttcttcta ggatattttc ctcattgaac tcatctacgg ttacttggcc agaaatcaag
    14521 tcccacatac tttccaaaac tttttgatca accttcaaag caattgcatc tgctttttgg
    14581 atatttgcga tcgtctgttg ataaatcaaa agctgttgcg aatataacag tatgctcagt
    14641 agcaaaggga taacagcaaa tagaagcatg gctttgtttg cttgggcaaa aatctgtttt
    14701 atgcttaaat tttttgtcat gatacttctc cagtcaataa tttctggttg ggatgttagc
    14761 tatattctgc tgggaaaaga cagtctcgga aataaaaata tctttaggta atgtttttcc
    14821 tgtttgaata tttccaaaat aacgagtaac ggtattggct acataccagc cgacatcagg
    14881 tttacactcg actacacaat tgacgattcc tgagcgtagc ttctcgatca tatcttcttg
    14941 tccatcaata gttacaatga tcaaatcttc tccaggtttg attgatgttt cttttatcgc
    15001 agctaaagca ccgtgagtca tttcatcact atggctgtat aatacatcga tttcggataa
    15061 atcatttgtt tctaaatatc gtttcatctg gtcttttcct ttcatccgaa taaaatcacc
    15121 gacaattcgg tgaacaatag tcatcggagg tttatggtcg atggttccat ctttttttag
    15181 ggttggttta cgattgattg attccaaaaa tccttcggag cgtagcttgg tcggtgtgga
    15241 gttctctgaa ccagcaagtt ctaggattct gatttcctgt ttctttgaat ttgtgaagtg
    15301 attgctgaca aataaacccg cccgatttcc ttctgctttg aaactggggc caatgtgggt
    15361 gacgaaaagc tcgggatcat tcgtttgaat atgtctgtcg acaagtatga cagggatccc
    15421 tgctcgcttt gcttctttta atacagagtc ccacccggat tcttgcaaag gagtaaaaat
    15481 gatcatatcc actttatagg caatgaacgt ccgtatatct tggatttgcc gttcctgatt
    15541 catgtagcca tttcggtaga gaacttggta atcttctttt tcaagcgcag tgataattga
    15601 ttcagtatgc tttttccgcc agctactctc cgttcctgat tgcgaaaaac caacgactag
    15661 tgggtcaggt gcttcctttg tattaggtgt acaccccatc atttggtaag cgattacaac
    15721 aagggctaaa aaaaataaaa cacgtttcac tttaaatcac tccatctgga tttgttagta
    15781 ctatcttatc agataaaagg gaaaatcaaa gtcaaaagga atcaatcgaa aaaaatccaa
    15841 gaaacttaaa aaaatccaag acaaaaaaag tacacctaaa aaatttaaag taaatcccat
    15901 aaaaaaatca atgttaacgc tttctttatt agctatactc ggtgtatcaa ttgaaatggg
    15961 ggataaaaaa tgaaaaacaa atttagaaag attgttggga ctatcgctat tttgaccgca
    16021 agcattctat taagtgcgtg tggcaattca ggatctgcag atgatagtac ggggtatgta
    16081 ggaatcgcta tgccgaccaa atcagcagaa agatggatcg ctgatgggaa caacatggtc
    16141 tcggaattag aaaaactggg ttataaaaca gatcttcaat atggagaaga taaagtagaa
    16201 aatcaagtag cacaaataga aaatatgatt acaaaagggg tagatacact tgtgattgct
    16261 tctatcgatg gttcggcttt aactgatgtg ttagctaaag caaaagaagc agatatcaag
    16321 gtgatcgcct atgatcgttt actgatgaat tcagaaaatg tcgattatta cgcgactttt
    16381 gataattttg gagtaggtgt atcgcaagca gcttatattg aagagcactt aggattgaaa
    16441 gaaggaaaag gaccattcac gattgaatta tttggtggct cgcctgacga caacaacgca
    16501 ctaatcaatt acaacggagt gatgtctgtt ttacaaccat atatggacaa cggtcagttg
    16561 gtcgtgccat ctggacagac gagtttcagt caaatcgcta cgcttcgctg ggatggttct
    16621 acggcgcaag caaggatgga taatctgtta agcgcaaatt atactgacca aacattggat
    16681 gctgttttgt cgccatatga tccaataagt ttaggaatca tttcgtcatt aaaaggcgtg
    16741 gggtatggga gtgaaagtaa acctttacct gtgattactg gacaagatgc gacagttgct
    16801 ggcgttaaat cgatcattgc cggggaacaa acacaaacga tctttaaaga cacacgtatc
    16861 ttagcaaaaa atacgattga aatgatcaaa gcaatcagtg atggagaaga ggttccggtc
    16921 aatgatactg aaacttatga taatggggtg aaaactgtcc ctacgtattt agcaaataca
    16981 gtatctgtcg ataaagataa ttatcaagca gagctgatcg acaccgatta ttataaagaa
    17041 tccgatctga aaaattaaga ggaaccaaac aaggaactaa agagatctaa gtcagagaga
    17101 ggataagaat tatggcggat tgtattttaa aaatggcaga tatcaccaaa aaattttctg
    17161 gaatccgagc gttagattgc gtgaatctga tgattgaacg tggggaaatc catgcattgt
    17221 gcggagaaaa cggagcggga aaatcaacat tgatgaatgt cttgagcggc ttgtatccat
    17281 atggctccta tgacggggaa atcttttatg atggaaagct gtgtcaattt aaagatttga
    17341 aagatagtga agaaaaaggc atcgtcatca ttcatcaaga attagcacta agtccttatc
    17401 tttcaataaa agagaatatt tttctcggaa atgaacaagc agaaagaggc gtgattgatt
    17461 gggaactgac agaaaagaaa acatatgaac tgctgaataa agtcggtttg tctatcgacc
    17521 cgaatcttct agtatcccag attggcgtag ggcaacagca actagttgaa atagctaaag
    17581 cattatcaaa atccgtccgt ctgttgattt tggatgagcc taccgctgca ttgaatgaag
    17641 aagaaagcgc gaatttgctg agattgatcc gagagttgaa agagcaaggt gtaacctcca
    17701 tcattatttc acataagctg aatgaaatcg tggatatcgc agatcgaatc accattttac
    17761 gggatggaca gacgattgag acactagaaa atgaaatgat cagcgaagag catattattc
    17821 gcggaatggt gggcagagat ttagttgatc gttatcctga aagaaaaccg gatattggag
    17881 aagtttattt taaagtagaa gactggacgg tctatcaccc tcttgatcac gagcgaatca
    17941 taaacgacca catcaacttg catttgaaaa aaggagagat tgtaggaata gctggactga
    18001 tgggagcggg cagaactgaa ttcgctatga gtctatttgg acattcttat ggaagcaaga
    18061 tatccggacg tatattaaag gaaggggcag agatatcagt taagaccgtt ccttcagcca
    18121 ttgcaaacgg actcgcttat gtctcggagg accgaaaatc tttaggactg aatctgctga
    18181 tggatattcg ggaaaacgtg acgctgagca gtttggataa aataagtcaa aaaggagtat
    18241 tagataaaga aaaagaagta actgaggcag aaaatttccg gaaaaagatg cgtataaaaa
    18301 caaactccat ccatcaaatt gtcagtagtc ttagtggtgg gaatcaacaa aaagtcgttt
    18361 tatccaagtg gctgatgaca caacccgata ttcttttttt ggatgagcca acccgtggga
    18421 tagatgttgg ggcaaaatat gagatctaca cgattatcga agaaatggca gcaaatggca
    18481 aagctgtctg tcttatctct tctgaacttc ctgaaatatt agggatgtgt gatcgaatct
    18541 atacgatgaa cgaaggacgt attacaggag aagtctcgcg agaagatgca gatcaggaag
    18601 tcttgatgaa attaatgacg aaagaagagg tgggttaagt tggaacaggc aaaaaatctt
    18661 acggataaaa aagagaaaaa actaaatttg aaaagtagag cagctgatct ttttggtcgc
    18721 tacagcatgg tgatcatttt agcgggacta cttattgcgt ttcaattgat gacggatggt
    18781 attttctggc gtccattgaa tataactaac cttgttctgc aaaatagcca tatattagta
    18841 ctagctgcag ggatgttatt agttgtgctc ttaggtcatg tggatttgtc ggtagggtca
    18901 gtgatggcgt ttgttggtgc aattgcgggc atgatgatgg tcaatcgcca aatgaacccg
    18961 tggatgacag ttatcgcatg tcttgcagta ggcgggctga tcggcgcatg gcaaggtttt
    19021 tgggtagctt atgcagggat accggcgttt atcgtgactt tagcaggatt gctgatgttt
    19081 cgtggactga cacaagttgt tctaggaggc caatctctag caccttttcc agctgttttc
    19141 caaaaaatgt ctacgggtta tttaccagac ttaagcgaag gaagcagcct tcacttcttg
    19201 acattgattt taggaatgat catcacgatc gtgttagttg ttggtcaatg gcgaacaaga
    19261 aatcgtcgga aaaatcattt atttgaagtg gaatctccaa cagctttctt ggtaaaggcg
    19321 gtatttacag cagtcgttgt actaggaata acttatatct ttgcctctta tcaagggtat
    19381 ccgattattt tgatcattct tggtttgatc gttgcagcat atgggttttt gacaaataaa
    19441 acagtagcag gtcgacagat ttatgcgacg ggcggaaatc gaaaagcggc tgaactatca
    19501 gggatcaaaa caaagcgaat cacgttctgg gtattcgtca acatgggagt catggcagct
    19561 ttagctggat tagttttagc tgctcgattg aatgcggcaa cacctcaagc aggtacatcg
    19621 atggaactgg atgcaatggc ggcagtatat tttggtggtg cttcgacttc cggcggcatt
    19681 ggaacaatca tgggtgcgat tgtcggtggc ttggtcatgg gtgtattgaa caatggcatg
    19741 tcgattttag gcgtcggggt agactggcag caggcaatca aaggattgat cttgctttta
    19801 gccgttgtgc tagacttgta taataagaaa aaacgaacaa cgtaggagaa cgccggttat
    19861 tcagaaaaaa gactgtgaca agacatttgt cacagtcttt ttttcgctaa aagcgaacat
    19921 tcattgtttc gtttgtatag actaaaaata atatatgttt ttttaccatt cgtataaatt
    19981 ctctttttgc tcttgatttt tttgcttaat tgagttaaat tacatagggc gctaaaaaaa
    20041 gccttgatta aattaataat gttcgtgttt tgctggatgt gaacatcagg aggataatgt
    20101 aatgaaagta tttgattacg aagatattca attaattcct aataaatgta tcgtaaatag
    20161 ccgttctgaa tgtgatacaa cggtcacatt aggtaaacat acattcaaaa tgccagtagt
    20221 gcctgccaac atgcagacga ttattgatga aacaatcgca gaattcttag cggaaaatgg
    20281 ttatttctat atcatgcatc ggtttgatga agcagctcga attccgttta tcaaaaaaat
    20341 gaaaaagcgt ggattgatta cctcgatcag tgttggcgtc aagaaagagg aatattcctt
    20401 tatagagaag ttggcagaag aatcgctaaa tcctgattat atcactatcg acatcgcgca
    20461 tggacatgct aactcagtta ttgatatgat tcagcatatt aaaaaatatt tgcctgaaac
    20521 ttttgtgatt gccggtaatg ttggtacacc ggaagccgtc cgtgaattgg aaaatgcggg
    20581 cgcagatgca acaaaagtcg ggattggtcc aggaaaagta tgtattacaa aaatcaagac
    20641 aggctttggg actggaggct ggcaactggc agctcttaga tggtgtgcga aagcagctcg
    20701 caagccgatc atcgctgacg gtgggatcag aacacatggc gatattgcaa aatctgtccg
    20761 ttttggtgcg acaatggtca tgattggttc attgtttgcc ggtcacgagg aatctcctgg
    20821 cgaaacaaaa gtcgaaaatg gaatcgtgta taaagaatac tttggttctg cttcagaatt
    20881 ccaaaaaggc gaaaaacgaa atgttgaagg aaagaaaatc tggatccaac acaaaggatc
    20941 tttgaaagat acactagtgg aaatgcaaca agaccttcaa tcatctattt cttatgctgg
    21001 aggaagagat ctggaagcca ttcgaaaagt tgactatgtc atcgtgaaaa attcgatttt
    21061 taatggagac acaatctaaa aaataaataa aatattagga tgttgtgaaa aagttgtcct
    21121 gcattaagta ataagaacag ccaaacaaaa atagttcttt atatgttttg tttggctgga
    21181 attattatcg cagatacaag cttttgaaca tcattattca gaataaggga ctgtgataag
    21241 atttttgtca cagtccctta tgttttgcaa aaaaagtttt tcttttttct cgtaaattcg
    21301 gtaaaatcaa aagaaaaaat agacaataga aaagagaagc ttatgtggga aaaagtagag
    21361 aaaaaacaag gaccgattta tcgtcaaatt atggaacaga ttatgagaga aatcgaaaac
    21421 ggaaggatca atcctggaag tcaacttgaa tcagaacgaa aactagggca actttttggt
    21481 gtgaatcgtt ccaccgttgt ccatgcattg gaagagctta gggagcttgg ggtactaacg
    21541 agcaaacgtg gcagcggatg gtttgttaat cagactgaat gggggaaatt cgctgttcca
    21601 agaatcgatt ggcggcaaat gatctctcct cgttatgagc agacggactg gtatgagcaa
    21661 aaaataagag aagcaaagca gatccaaaaa acatcgtttc ttgatttata cgctagcgag
    21721 atgccagatg agcttttgcc gaattttgag tttccttctt attctcttga agaaatctta
    21781 gcggaagaaa aagaaatgga tcttttaggc tatactccgt tacgtaacaa aatcaaacgg
    21841 tatttggaaa gtaaatttga attggcattt tcccccaacc agcttcttgt gactggtggc
    21901 ggtcagcaag caatcttttt gattttacaa acgattcttt cagctggaga ggggatagct
    21961 gtagagtcac cttctttttt ttaccgatta cctcttttca aagcttcagg aacgcgttta
    22021 tttgggatac cgatggatga agaagggatc gacttagaaa aactgaaaga atcgatccat
    22081 aaaaataaat tgaaagcagt gcttatcaac ccgaattttc aaaaccctac aggaaaagtg
    22141 atgagtcaaa aacgacgaga aaagctagta gaattgtgtc gacagtatcg gcttccaatt
    22201 attgaagacg atgtatttac ggatctttca tttctaaacc aagaagaagt ccttcctata
    22261 cgaacgattg atccggataa tgtgttatac gtcggttcgt tatctagggt tttaggaaaa
    22321 acaacgaaaa tcggttgggt gataggacct gagactttta tctcgcaact agctcaagcg
    22381 caagaaatga tggagttttc gatgaatatc cttacacaaa tcacagtggc taatgtattt
    22441 aatgaagcaa ttgatttgaa aatgcaagat gtaagaaaaa agttgaaaga aaaaagccga
    22501 ctgataagcg cttggggaga ttcacaatcg tttttcaagc ttcattcaat tttaggtggt
    22561 tactatgcct ggattacttg ggatgggaaa aaactagacc ccgaactagc agaaaaagta
    22621 gtagatatgg gtcttggaat tgcaccaagt ctactttttg ggcaagatat aaatggaatc
    22681 agaatcaatt acagtaggct ggatgaaaac caaataccgt tgttttattc aaagatgaaa
    22741 attctagaaa agtggttaac agaataattt cacaaagtaa atagattaaa gaatataaca
    22801 gcttggattt aaatttcaag ctgtttcttt tttttcggaa aaagaatacg atataaagaa
    22861 actaagaaaa aaccattatt tgtacgtccg ttcacgatgt gtaaataaaa taaatgtttg
    22921 caacaaaact gtataacacc aacgttttta aatagaaaca gatggttgta agtttgatta
    22981 gaaaatagaa aaaatgcaga tgaaatataa aacgctttcc ttattgtgat tttattcact
    23041 aatgctgtta atatgcggat tggcataatt tcctttcatc gtgaaaattt gtgcttttag
    23101 cttgtatttc tcataaatcc atgataattt attaatgaag acggttaaat agtgatacag
    23161 ttttattcac tgtttcaatc gcagaatgaa gagattttct tatagatgag agaggaatgt
    23221 gtgacaaatg gcaaacaaaa aagcaccgat cgacttccaa gcactgctag atgaagtcaa
    23281 tgcagatttc ccagtatatc aagcacttga tcaagatgga aacgtagtta acaaagattt
    23341 agtacctgat ttatccgatg atgaactcgt tgaattaatg actcatatgg tatggtctcg
    23401 tgtattagac caacgttcaa cagcgctgaa ccgccaagga cgcctaggct tttttgcacc
    23461 aactgctgga caagaggcaa gtcagctggc tagtgcttac gcatttgata aagaagatgt
    23521 tcttcttcca ggttatcggg atgtacctca actagtaaaa catggtttac ctttatctca
    23581 agctttttta tggtctcgag gacatgctgc aggtaattct tatccggaag aattgaaagc
    23641 tctaccacca caaattatca tcggtgcaca atatgtacaa gcagccggtg ttgcattagg
    23701 tttgaagaaa cgaaacaaga aaaatgtcgt cttcacttat actggtgatg gcggttcatc
    23761 acaaggtgat ttttacgaag caatcaattt cgctggcgct taccatgcaa atgccgtatt
    23821 ttatatccaa aacaacgggt atgcgatctc tacacctcgt gaaaaacaaa ctgcagcaaa
    23881 aacacttgca caaaaagctg tcgcagctgg gattcctggt attcaagtcg atggaatgga
    23941 tccgcttgca gtatatactg tatctaaaat ggcacgggaa tgggcgatca gtggaaatgg
    24001 accagtccta atcgaaacac tgacttatcg ttatggacca catacactat ctggtgacga
    24061 tccaacccgc taccgtacaa aagacacaga tgacgattgg cagaaaaaag atccattgat
    24121 ccgcttccgt aaatatttga cggaaaaagg actttggtct gaagaaaaag aagaacaagt
    24181 catcgaagca actaaagaag aaatcaaagc agctattgct gaagccgaca aagtaccaaa
    24241 acaaaaggtt tcagatttct tgaaaaacat gttcgaagta tctccacaat caatcaaaga
    24301 acaaattgca atttatgaag cgaaggagtc gaaataatca tggcacaaaa aacaatgatc
    24361 caagcaatca cagatgcctt agcacttgaa ctagaaaatg acgagaatgt cgttgttttt
    24421 ggtgaagatg ttgggaaaaa cggaggagtt ttccgggcaa ctgaaggatt gcaggaaaag
    24481 ttcggagaag accgagtatt cgatactcct ttagctgaat ctggaatcgc aggattgagt
    24541 ttcggtcttg cgttagaagg atttcgtcca gttcctgaaa tccaattctt tggttttatt
    24601 ttcgaagcaa tggacgaagt agtagctcaa atggctcgta caagatatcg aatgagcggc
    24661 actagaaatc tacccatcac aattcgttcg ccatttggtg gcggtgttca tacaccagaa
    24721 ttgcattcgg ataatttaga aggtttgatc gctcagtctc ctggtattcg agtagtgatt
    24781 ccttccaacc cttatgatgc aaaaggacta ttgatttctt ctattagaag taacgatccg
    24841 gttgttttcc tagaacacat gaaactatat cgctcattcc gagaagaagt gccggatgaa
    24901 gcttacgaag ttcctttgga taaagcagca gttactcgtg agggaacaga tgtatctatt
    24961 atcacatacg gagcaatggt gcgtgaagca atcaaagcag cagacaatct tgcaaaagaa
    25021 aatatatctg ttgaaatcat tgatcttcgt acagttgcgc cattagatgt agaaacgatc
    25081 attcaatccg tggaaaaaac tggtcgcgta gttgttgtcc aagaagcaca acgacaagcg
    25141 ggcgttgccg cgcaagtagt ttctgagatc tcagaacgcg ctatcctttc attagaagcc
    25201 ccaatcggac gagtttctgc acctgataca gtcttcccat ttggacaagc agaaaatgtc
    25261 tggttgccaa atgcaaaaga tatcgaagac aaagtaaaag aaatcgcgga attttaagaa
    25321 aagaagggac gaaacaaagt catggcttat caatttaaat tgccggatat cggtgaagga
    25381 atcgcagaag gtgaaatcgt caaatggttc gtcaaaccag gagatacgat caatgaagat
    25441 gatacattac tagaagttca aaatgacaaa tcagtagaag aaattccgtc acctgtcaca
    25501 gggacagtta aaaacgtgat tgttccagaa ggcactgtag caaatgttgg ggatgtatta
    25561 gtagaaatcg atgctcctgg acacaatagt gcaccgtcaa ctagtgcgcc atcagctgaa
    25621 gcaccaaaag aaaaagtgga aacatcaggt tcagcaagtg tcgttgaagc tgccgatcca
    25681 aataaacgtg tattggctat gccatctgtt cgtcaatttg cccgtgaaaa agatgtagat
    25741 atctcacaag taactgcaac tggaaaaggt ggcagagtaa caaaagagga catcgaaaac
    25801 ttcttggcag gtggtccaag cagtgcacca gcgaaatcag aagcgccaga agcggcagca
    25861 ccgaaagaag cagctcctgc agcagaaagc aaaccagctg caccagcaaa accattcaag
    25921 tccaacttag gagacttgga agaacgtgta gcaatgacgc caacacgtaa agctatcgct
    25981 aaagcaatgg tcaacagtaa gcatacagct ccacacgtga cattgcatga tgaagtagaa
    26041 gtgtctaaac tatgggataa tcggaaacga tttaaagaag ttgctgctgc aaatggtacg
    26101 aaacttactt tcttgccata tgtcgtgaaa gctttaacag cgactgttaa gaaataccca
    26161 gtattgaatg catctatcga cgatgcaaac caagaaatcg tttataaaca ttactacaat
    26221 atcggtattg caactgatac ggatcacgga ctatatgtac caaatgtgaa agatgcagac
    26281 cgcaaaggaa tgtttgctat tgcagacgaa atcaatgaga aagcaaaatt agctcatgac
    26341 ggcaaactat ctgcagaaga tatgcgcaat ggaacaatta cgatcagcaa tatcggatct
    26401 gtaggtggcg gttggtttac accagtcatc aactatccag aagtagctat tttaggtgta
    26461 ggaacgattg cacaacaacc aatcgtaaat gctgaaggcg aaatcgttgt cggtcgtgtg
    26521 atgaaactgt cattaagttt cgatcaccgt atcgtagatg gagcaactgc gcaacaagca
    26581 atgaataata tcaaacgtct tttagctgat ccagagctat taatgatgga aggatgataa
    26641 acaaatggta gttggagatt ttgctgtaga attagataca gttgtaattg gtgcgggacc
    26701 tggtggctat gtagctgcca tccgcgctgc tgaaatggga caaaaagtag caatcattga
    26761 aagagagtat attggcggtg tttgtttgaa tgtcggctgt attccttcaa aagcactgat
    26821 agctgcagga caccattatc aagagtcact agattctgaa atgtttggag taactagtga
    26881 aaacgtcaaa ctagattttg caaaaacaca agaatggaaa gaaaacaaag ttgtccatac
    26941 gttgacttct ggtgttggtt tcctattaaa aaaacataag gttgaaacaa tcgaaggaga
    27001 agcgttcttc gtagatgatc acacactacg cgtcattcat ccagattcag ctcaaactta
    27061 ttcgttcaat aacgcaatca tcgcaacagg ttcccgtcca atcgagattc caggatttaa
    27121 attcggagga cgtgtgttag attctactgg cggattagca ttgaaagaag tacctaagaa
    27181 atttgtgatc atcggtggcg gtgtgattgg tgccgaattg ggcggagcgt acgcaaactt
    27241 aggagcagaa gtaacgattc ttgaaggctc accacaaatt ttaccaactt acgaaaaaga
    27301 tctagtaaaa ttagtagaag atgatttcaa gaaaaaaggc gtaacagtcg taacgaatgc
    27361 aatggcgaaa gaagcagtcg ataacggtga cagcgtgaca gtgaaatatg cagtggacgg
    27421 gaaagaagaa tccgttactg ctgattatgt gatggtcact gtaggtcgtc gtccgaatac
    27481 agatgatatg ggcttagaac aagcgggtgt cgaagtagga gaacgaggat tgatcacggt
    27541 ggataaacaa ggacgtacga acgtacctaa catctatgcc atcggtgata tcgttccagg
    27601 ggcggcatta gcacacaaag ccagttatga agcaaaaatc gctgctgaag caatctctgg
    27661 caaaaaagta gctgtagatt ataaagcaat gccggcagta gccttcactg atcctgaatt
    27721 ggcttctgta ggtatgacga tcaaagaagc aaaagatgct ggtatcgaag caaaagcata
    27781 taaattccca ttttcaggga acggtcgggc attatcgctt ggtaaaacag aaggattcat
    27841 ccgtctagtt acgacgatcg aagataacgt gcttattggc gcgcaaatcg gtggagtcgg
    27901 cgcaagtgat atggtctctg aattagcttt agcaatcgaa tcaggaatga atgctgaaga
    27961 tatcgccttg acgattcatc ctcatccatc tcttggcgaa atcgtcatgg atgcttcgga
    28021 attagcatta ggtttaccaa tccatatttg atttgaataa tacaggcgaa acgcaactga
    28081 aaaatcagtt gcgttttgtt ttttagaatt atcaataata ttattatgta aacttaaaaa
    28141 tatgacaact tcttggttta ttaccacttc ctatgctaca ctgaagagga atatgaaata
    28201 ggagagttga agatgcaaga atatcagtat ccacttgatt tagattggac aacagacgaa
    28261 atggtcattg taatgaagat gtgggaagat ctggaacgtg cgaatgaaaa agggataaac
    28321 aatgaagctt ttttgaagac ttatcagcag tttaaaactg ttgtcaaatc cattggtgag
    28381 gaaaaacgat tgggacggga atttgaaaaa gcttccggtt attctttata tcgtacagtg
    28441 cagcaagcaa aaaaaaatcc aaacaaaact ttgagaatga acgggtgaga taaatggaac
    28501 agatgattga tcatatcaag tcctggcttg aagaagctgg tgagaaaatc aaatcttctc
    28561 aacaaacatt aacaatctcg caaaaatcaa atcgaacgga tctagtaaca aatgtcgatc
    28621 gaaacatcca gaattttcta atcgaaaaaa tccattcctt tgatccagag gcgaaaatat
    28681 tggcagaaga agaagggtgt aatcaattat cggatctcga tggaagagtc tttatcatcg
    28741 atcctattga tggcaccttg aattttgtga ttgaagggga aaatttttgt attatgctcg
    28801 ctgtttatga atccggcgtt ggaaagcttg gtttcattta tgacgtgatg agaaatgaac
    28861 ttttttgggg tggaaaagga ataggcgtgt ataaaaatga tgagccattg cctagaccag
    28921 caaataaatc cttatcggaa ggcttacttg gtgtgaatag ttatcttttc ggtcataata
    28981 aatatggaat ccgagaaatt gcggaaagca gcatggggac acgtatgtac ggatgcgctg
    29041 gtcttgaact agtggcaatc ttgaaaggaa accacattgc ttatatttca aaccttagcc
    29101 cttgggatta tgcagcggga aatgtcttat tggaagagtt tggtatggta acaagcggtc
    29161 tttttggtac tcctttgaca tttgaaaaaa gggaatacta tttagcaggg acttcttgtg
    29221 catatgcaga aatcgcagac cttttgaaaa aagaaggctg aaaaaaaggt agaaatcatt
    29281 cacaaaaatg ataaaaacga gcaaaagatt aattttctcg aaaaaatgaa aacattttac
    29341 tatatttcat gaaaaaagtc tgctataatt agcagtcgag taaaatttgc cctgtcataa
    29401 agaaccaata aaaaaggagt tagttacatt gaattataga aacgatatcc gtaacgtcgc
    29461 aattattgcc cacgttgacc acggaaaaac aacattagtt gatgaactat taaaacaatc
    29521 agatacatta gacgcacata cgcaattgca agaacgtgcg atggactcaa atgcgctaga
    29581 aaaagaacgt ggtatcacga ttttagccaa aaacacagct gtagactata aaggtattcg
    29641 cgtaaacatc atggatactc caggacacgc ggacttcggt ggcgaagtag aacggatcat
    29701 gaaaatggtt gacggcgttg tgttggtcgt agatgcgtat gaaggaacaa tgcctcaaac
    29761 acgttttgta ttgaaaaaag cactggaaca acatatcaca ccaatcgttg tcgtaaacaa
    29821 gattgacaaa ccttctgctc gtcctgagca tgtcgtagat gaagttcttg aactattcat
    29881 cgaattagga gcagatgatg atcagttaga ctttcctgtg atttatgcat ctgcactaaa
    29941 tggtacgtca agcttgtcag atgatccagc agatcaagaa ccaaccatgg cacctatttt
    30001 tgatacaatt attgaaaaaa tccctgctcc tgtcgataac agcgacgaac cattacaatt
    30061 ccaagtttca ttacttgact acaacgatta tgtaggtcgt atcggaatcg gacgtgtatt
    30121 tagaggaaca atcaaagtag gagaccaagt agcattaatt aaattagacg gaacagttaa
    30181 gaaattccgt gtaacgaaat tatttggttt ctttggacta aaacgtctag agattcaaga
    30241 agcaaaagct ggagacttga ttgctgtctc aggtatggaa gatatctttg tcggtgaaac
    30301 agttactcct gtggatcatc aagatgcgtt acctatcctt cacattgacg aaccaacatt
    30361 gcaaatgact ttcttagtta ataattcacc atttgccggt cgagaaggta aattcgtaac
    30421 cgctcgtaag attgaagaac gtttgatggc agaacttcaa acagatgtat ccttacgagt
    30481 tgaaccaaca aactctccag atgcatggac agtttctggt cgtggagaat tgcatttgtc
    30541 tatcttgatt gaaaacatgc gccgtgaggg atacgaattg caagtatctc gtccagaagt
    30601 tattgaaaaa gaaatcgacg gtgtaaaatg cgaaccgttt gaacgcgtgc aaatcgatac
    30661 accagaagaa tatatgggta gtgttatcga gtccttgagc ttacgtaaag gtgaaatgca
    30721 agatatggtc catacaggaa acggacaaat ccgtttgaca ttcttaacac ctgcccgcgg
    30781 tttgattggt tattcaactg aattcctttc aatgacacgt ggttacggaa tcatgaacca
    30841 tacttttgac caatatctgc caatgcttcc tggtcaaatt ggcggacgtc accaaggggc
    30901 gcttgtttcg atcgatacag gaaaagcaac gacttattct atcatgagta tcgaagaacg
    30961 cggtacagtc ttcgtagaac caggtacaga agtatatgaa ggaatgatta tcggggaaaa
    31021 cagccgtgat aatgatttga ctgtcaacat cacgaaagca aaacaaatga caaacgtacg
    31081 gtctgctact aaagaccaaa catctgtcat caaaaaacca aaacaattga cgttggaaga
    31141 atctttggaa ttcttgaatg atgatgaata ttgcgaagta acaccagaaa gcattcgctt
    31201 gagaaaacaa attttagaga aaaatgctcg tgaaaaagca tctaaaaaga aaaaataatc
    31261 tgtaaaagga tgaagagaga aaccagcttg ttata
//
LOCUS       DACECY010000031        30525 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.032, whole genome shotgun sequence.
ACCESSION   DACECY010000031 DACECY010000000
VERSION     DACECY010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.032"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1638)
                     /locus_tag="IWO38_001495"
     CDS             complement(<1..1638)
                     /locus_tag="IWO38_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAQ0313589.1"
                     /translation="MATVILAEKPDQAEKFAFALADKKIPKSGGKFEFQSEVFGDTIV
                     TWGIGHLVGLSMPEKYEWLPNKEKWDLANLPFLPKMDELKYEVSKGKSQQFTVVKSCL
                     ENADQIIIATDPDREGENIAYNIFKLCKQSIFKKPMKRLWINSLVKSEIIKGFKNLRN
                     AEETISFFEEANARQIADYLVGMNYTELYTLKLQQKGLSGVVSLGRVQTPVNTLVVEN
                     DLSIKNFKSEKYKVIECHTVDREPKVIFKNSREYFKLEKFEEDTKKFGLDSASTGIIK
                     KIEVSKKQTEPQKLFSLGGIQEYANKKWKYSAKKTDKIIQSLYDRGYLSYPRTDSELI
                     TTNEFEYLKANLDVYKSVLGISIDTPCLEPKKRFVDNDKVLEHYALIPTMDIPDIANL
                     TLDEKNIYEIVTRRACLMFAEPYKYENTKVTLDVNGLEFTTSGNVPISLGWKQVDTNL
                     DESDDKEETLPKFHEQETINIDIRFIDKLTKEPKRLTEGKLLGKTGIMAKLGLGTPAT
                     RSNIVDTLLNREYIKIENTNVYPTPKGYLLWDLTKNKD"
     gene            complement(1653..1871)
                     /locus_tag="IWO38_001496"
     CDS             complement(1653..1871)
                     /locus_tag="IWO38_001496"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313590.1"
                     /translation="MELIPYVYQHLYKAKSMKDGNEVVGSLICCPPFSYIATIESMKK
                     MCVDELNDGEVKNLKLTRVLERSIEKFK"
     gene            complement(1888..2247)
                     /locus_tag="IWO38_001497"
     CDS             complement(1888..2247)
                     /locus_tag="IWO38_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HAQ0313591.1"
                     /translation="MNIDGIYNESDFKKGDIAVIRSEYDFPSEIGIHTVEGETVCRYW
                     GRKGNLILLLDTDSGEKLKFCFWHNTNYCCEPIPKRLIDVNKESVRYLADGSRIRLTY
                     QKSENSSITRVRDIVLI"
     gene            complement(2383..2682)
                     /locus_tag="IWO38_001498"
     CDS             complement(2383..2682)
                     /locus_tag="IWO38_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ0313592.1"
                     /translation="MVIKKYVYSHPINVFIIGKLGLSVEVFCELYGFKQGTLSSWITR
                     EREVADLPISFLYALSLASGGNMNEVYDCLCEYEKEYKEYLKISAPKRNKKYIED"
     gene            complement(2916..4517)
                     /locus_tag="IWO38_001499"
     CDS             complement(2916..4517)
                     /locus_tag="IWO38_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ0313593.1"
                     /translation="MLNSCEYADVLAKILKNGIKQFNSKNLVAFKKNSNKIGAIAGFT
                     SKTNMIKTKGLIYRSIDRVLEDSDVLTHWTPNVYSWLGGGENKTIFGHKEANLIQINT
                     FVADIDYPVGEKKTDLNLLVLYLLEEGLLPTLIVDTPKGYHCYFLIQNYNQETDSFDK
                     ASYISNSNDFKSLRVAKRISENIRKSIQNRLPQVDMGCNHFGIFRFPTKKNIVHYEPN
                     FVDTFEGYLKWSKDFEEKEYALKRSNLSLLAPNSTGKKGSKRQIDTKWYDFLIHTDIA
                     EGDRNKTIFTLALACKSSGLSIGECMDQLDQLQFNNDLSYSEVERTIRSAYRGDYKGA
                     KSSYVNEIIENYATPTEFEKYSINVNRQAVKRKGNRVDPSNWVKFAKPREERKYSHFE
                     ESKADLLAYLERKQRSLKKNELFLSVSMGTISEETQIPVSTLKVILKELRTANTVILR
                     TSRGRYGSTKIATSKFVEAKILNTVIQNKKDKQVFVLELLGTANSHVETILRRYEDKT
                     VPFHANILKEREKSKHAPIRGANTG"
     gene            complement(4914..5168)
                     /locus_tag="IWO38_001500"
     CDS             complement(4914..5168)
                     /locus_tag="IWO38_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313594.1"
                     /translation="MYNVGDQIDEIVKIGNVKLVKSKYYISETEIIFNTESGCILIWK
                     LPFPKDETTLKKINTLKIRYCIHEIIDKNTYVINQVKVMG"
     gene            complement(5667..6551)
                     /locus_tag="IWO38_001501"
     CDS             complement(5667..6551)
                     /locus_tag="IWO38_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HAQ0313595.1"
                     /translation="MKRFKGSKMSKKIGKESLSLGLKKEIVKFKSMYREPEFRLIFQL
                     DIVRGLKISFSCLGYLVWLFIARVGWSNAYSFYKLFAGTDRLKESLEVKEALNILGSL
                     AFSLFIFALFILQVGKIMMSTNDNYINYLKPFQKGIKIICRQGTVLFLLSLYEHEIHF
                     QNYYNTTTKLGAFLLLYLVSNIAEKMLFKHIVKQVFSDIQKESYCLKTPYGNLKNVEL
                     FLFKMDSKWDKYIYRSKEVFLTGSYNKTLQESLYDTGAIFNNEPMVLKDFFGDYELVW
                     NPKQLSEQGAENEFAEVH"
     gene            complement(6538..7101)
                     /locus_tag="IWO38_001502"
     CDS             complement(6538..7101)
                     /locus_tag="IWO38_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313596.1"
                     /translation="MTAIYKALKNSNVSEYVKSRVLNNNLDYILENGVADLISKLSDP
                     IDIDHLVVWQDGTLEVSIYGEFYSRNKVTTFKNSISGREDLKGINKNSKFYRTNKELL
                     EKISKRVFELVNPFSKKRSKELFGIFYDGFSAKSNVEKFFYFNDSAYIAVKDSKRIPE
                     ATLEYLEPIKLSVFYSLYEKEIADEAV"
     gene            complement(7597..7905)
                     /locus_tag="IWO38_001503"
     CDS             complement(7597..7905)
                     /locus_tag="IWO38_001503"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313597.1"
                     /translation="MKLEIKEVVCDWGIYVDGETYPFMIFNSKANAQEIMRIMELDNK
                     HERFDSVKKMENECEAVEYQKTCANNKYILGAMGLGKSASIKQVYEESQENCVYENSN
                     "
     gene            complement(8349..8960)
                     /locus_tag="IWO38_001504"
     CDS             complement(8349..8960)
                     /locus_tag="IWO38_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0313598.1"
                     /translation="MLVGYARVSSDDQNLERQLEEFRKIGVEKVFAEKKSGRNTVERP
                     EFNKMLSFVREGDTLVFESLERLGRNSDEILETVSFIDKNGMGLMVLNLPILDTKFGD
                     PNLEKLVRSLVINIFSWTAQNEREEIKRKQKQGIAIAKKKGVYKGKPYEYSSTAKNPQ
                     KRFIYNEIVRMLGEGVPIKRIAEKTGTNRNLVYRIKKRISESD"
     gene            complement(9000..9782)
                     /locus_tag="IWO38_001505"
     CDS             complement(9000..9782)
                     /locus_tag="IWO38_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0313599.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSNTFSLNKDYELLNPIIKNIFTKYYSKDFEDYRDIEVKPDPKKVGRKIRSIRK
                     KLGLTMQEFGYEVSNSPRSTVSTWEHGGNLPNKAKLKKIADIANCSVEDLLFDEGFIS
                     EKSQVKLINELKEENSKLKEKIKYYECLFDGIESLLNSRKNS"
     gene            complement(9796..11178)
                     /locus_tag="IWO38_001506"
     CDS             complement(9796..11178)
                     /locus_tag="IWO38_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0313600.1"
                     /translation="MKPNLSLTSERIRKIIESKGLTLERFGDLIGDTPISSVNNWLRG
                     VSLPNKNRLLTIANIGNTSVDWIKWGTLEEYIASYLIDSGYELYIKDFPEIPYKVFKD
                     IQEKYSDTFSLDKDYELLNPIIKFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTT
                     LTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNVELKNLNIGNNIKNIRKSKN
                     MTQTDFAELMGLSRSYIGDLENNRRNPSIKTLEAIARALDVPIVALINDSSTDNIVEP
                     YESLTAENILLKQENEELKYEVESLRKRLYEIQQVVNQGAEKMIPNLKDVSKRIKYIR
                     KKLGMTMEQFGSHLSNSPKSTIATWESGRNIPSQKKLQLIAIVGETTTDWIKWGTLEE
                     YITSYLIGIGYELYIKDFPEIPHKVFKDIQEKYSDTFSLDKDYELLDSIIKNIFTKYY
                     SKEFEVTQIP"
     gene            complement(11227..12123)
                     /locus_tag="IWO38_001507"
     CDS             complement(11227..12123)
                     /locus_tag="IWO38_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0313601.1"
                     /translation="MEINLFEAGNRIKQIREKHGYTMADLGKLVDANSPSTINNWEKG
                     NNLPNRKRLEKIALLGGTSVEWIKYGDFSDYVYRLTQNIEEKILKEYGETTLNNYREA
                     LLDELTIEKITYEEDTKILAAAEKINTQNIFQEVKYMYSPDEDYRNIEVKPDLGLTDD
                     NKNIKMIGQRIRKIRKKENITLEEFGKLFSPTADKAVISNWENGKNLPNSERIKKIAE
                     IGGVSELYLMTGVDSSVAESMYFLSEVALDALERLSIEEVNRIIESFAGYLNVISKID
                     DPDKREISLNAICQLSLLDKMG"
     gene            complement(12318..12860)
                     /locus_tag="IWO38_001508"
     CDS             complement(12318..12860)
                     /locus_tag="IWO38_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0313602.1"
                     /translation="MKFGGHFLNKKQDQEYYIEKLEKENLELKERIRYYETKFCKRGD
                     CMKPNPVETGKRIKSIRSNFGMTMEQFADLIGNAQKGNINNWERGFSLPNKERLIKIA
                     ELSNKTIDWIKWGTLEEYITSYLIGIGYELYIKDFPEIPHKVFKDIQEKYSDTFSLDK
                     DYELLDSIIKNIFTKYYSNG"
     gene            13031..13219
                     /locus_tag="IWO38_001509"
     CDS             13031..13219
                     /locus_tag="IWO38_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAQ0313603.1"
                     /translation="MPLTGKKMLKLAKKNGWKVVRITGSHHIVAKEGFPPVPIPVHSN
                     QDLKKGTEQKLLKDLGLK"
     gene            13265..13642
                     /locus_tag="IWO38_001510"
     CDS             13265..13642
                     /locus_tag="IWO38_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HicB family protein"
                     /protein_id="HAQ0313604.1"
                     /translation="MKKSYPVIFSAEENGYSVVFPDFAGATQGDNFEEAMSNAKEFLD
                     GILAYYIDEDISVPSPTDIHQLNNLGNNKIVSLIQSDPSPFIQKKTVRKNVTVPEWLV
                     ARGEKDKINFSKVLTDALLKIYE"
     gene            complement(14268..15440)
                     /locus_tag="IWO38_001511"
     CDS             complement(14268..15440)
                     /locus_tag="IWO38_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313605.1"
                     /translation="MTKKIEVYVDGSQEERDSFFGFFWKNSREYVKALNMVYKQLMFE
                     ELIENSIKELDHEYIKRELSLEERIDNLLSNEVIDQKKLDKTKRELSKLRIAKNKEAR
                     DVLTQAIGLKSQTRIRDIAKKLDFDYSDFIDSAKMKASKDFSNDFVGIVTGQRTPRIY
                     RNKNKYGLLPFRGRDIKIHKADKFYISLPKGFRFKVNLGRKPRKARDSALTLERIMDG
                     QYKLNQSNLIFEGKNLFLNLTISFEKDYHKLDFKEGAELTIMFTDLCSGKCLVNNQSV
                     IDFGDSKYISNFKNKIKAMKSREQSRGVFSKGGHGRKRKLDQDRWEAIKKRESNFVKT
                     YNNQCSAKIIQTAIRNKTQIICISEPDKNYGEWAYYQLKEQIINKGKRYGIEVKSE"
     gene            complement(15684..16652)
                     /locus_tag="IWO38_001512"
     CDS             complement(15684..16652)
                     /locus_tag="IWO38_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ0313606.1"
                     /translation="MILENDKPYYVVIGGVNGAGKSTIYQIQNSKRKELDTDRINADE
                     ILRRFGGNWNKQSDINKSMREVLREIEGHFKEGKSFHHETTFGGNPYPDKKRLLNAKK
                     LGYESFLIYIGLDSPERAIERVKSRVAKGGHGVPEDLIRKRYEFSLRNLAELEKYFDN
                     VSIYDNSDEFSYIYVKENHTVVANNTQEYAWVPKAIQEKNAETPISKQIMMDISQEEM
                     NPKETLERIYSASDDFISDRFSKEAQVDDFYRNHKNEIKERILSISFSTGVSQDELVG
                     QDKRRNSCKLAYINSVGELLVKTDSELLSMELNRDRNIQKTDFEVE"
     gene            complement(16649..16846)
                     /locus_tag="IWO38_001513"
     CDS             complement(16649..16846)
                     /locus_tag="IWO38_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313607.1"
                     /translation="MRDMTEAVKELKKMYPDVLNMTVDDFHEALKNAESEEERTFYLV
                     LSSYVTRVDQKKVINQKDFKI"
     gene            complement(16857..17174)
                     /locus_tag="IWO38_001514"
     CDS             complement(16857..17174)
                     /locus_tag="IWO38_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313608.1"
                     /translation="MKDYASRKFIHLEPMKELVINGNEYNDYKSLFVKEQPKGGIRMK
                     NLPDFRLIEVNNLGIIEDYLAKLDEAVKQSIEKNGEDHVLTVVLKDLKDRLEAEAENF
                     NKD"
     gene            complement(17211..17429)
                     /locus_tag="IWO38_001515"
     CDS             complement(17211..17429)
                     /locus_tag="IWO38_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313609.1"
                     /translation="MVNKKETFKVGMEVQGVVIGSGELIEGEVISLLANVAVVKANGK
                     APVVMKKCSMMCLDGLNNPITQRFENVI"
     gene            complement(17538..17729)
                     /locus_tag="IWO38_001516"
     CDS             complement(17538..17729)
                     /locus_tag="IWO38_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313610.1"
                     /translation="MEQTRMFWSDRMQDHLDCIYKTREQLNKLDTVRYYFTYFSENDE
                     QRWDIFHDGNYEEFFYTES"
     gene            complement(17891..18247)
                     /locus_tag="IWO38_001517"
     CDS             complement(17891..18247)
                     /locus_tag="IWO38_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313611.1"
                     /translation="MNDTIAILSDSLIDLDKTSTDYMIIEEQLKNKNPEFDIQEKSLR
                     IELPKFKQKKLITILFEIIERYGLTYYQIWTDKKDGQYTKGVSIEAVREDDQVSIKAV
                     QPLMGYNEELLNCLNV"
     gene            complement(18272..19276)
                     /locus_tag="IWO38_001518"
     CDS             complement(18272..19276)
                     /locus_tag="IWO38_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HAQ0313612.1"
                     /translation="MDLKKAKKVSIVDFCKQNGIGLKQDSREYYRLEDHDSCVISDRK
                     NLFKWNSKDLGGDIFDFIKAYYDCSFKEAKQKLEDSDYEVHSFNTKVKKAPYQYQKEN
                     EVPVSVAKSYLVNERRIDTALVEDFLSKGLIKQDKKGNVLFLWKKDQEIVGCTEQGTR
                     KFFHEGKQKDVTWKKIQENSKEYSGFNVTFGQPDKMYFFESEIDLMSYITQKPEKAEN
                     ATFVSMNGLKVGNVLHGVTEHYNKFKSLPSETVLCVDNDKAGRTFIETEFKEKGIGRE
                     GIGISEVKVDVPLREGFDWNDNLTRGKEAFKRFKLEDVSKIVNFTHKLSLNTPVYEME
                     "
     gene            complement(19313..19573)
                     /locus_tag="IWO38_001519"
     CDS             complement(19313..19573)
                     /locus_tag="IWO38_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313613.1"
                     /translation="MIEQENRNFLETQGEYRQINNSHFVICEDVQIAFKMALKYGKSI
                     RKKIIREKKSKKIGFWELSLRDGLNFIFCTEDYYEKYKCTLS"
     gene            complement(19582..20655)
                     /locus_tag="IWO38_001520"
     CDS             complement(19582..20655)
                     /locus_tag="IWO38_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313614.1"
                     /translation="MSNEQNYETGGTYRLFHGSMNKFDEFSLDHKGLNGTANGFGVYL
                     TPNKEMATMYAEQGSNGNNGFVYEVNVELEKPISMNERTITDEELSKVIDKLQESNDI
                     LNDYNDVSYYGEKVVKREVIASLSENENDVDLINDIGNTIGDHEATAKAFYDVGKYTH
                     IIAEEQLRQKDVVIVVLQPDKVKIETIHDLNEERNSLESSEDMTDKFVGHKVSEVPIR
                     EVVIKDFEQELETTNYYDKQAWQERIEYLENMEEPVSRLALDSDINEFYDSHKEEIET
                     KLTNLSEKLEIDKEELLGFPVDTSSTEYKKATSIMAYNGAMREIYNEIENDTFEIPPV
                     SEEMENKILKLTNFENTTKFEME"
     gene            complement(20663..22489)
                     /locus_tag="IWO38_001521"
     CDS             complement(20663..22489)
                     /locus_tag="IWO38_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0313615.1"
                     /translation="MAYKKGNWKPKTKEQKQKELDDLIKASNSKIEQYQSNPEDMIEF
                     AKFMSKIHNYSPLNLSLIDEGFEGAIAVASYDGWKKLGFQVKAKEKGIGIYAHAPVTV
                     FIDENGEEKTLKEATSEQKEKIKNGELRSKKIKHFKKGYVFDISQTNATEADLPKIFP
                     NRVWNFNIDGELSVSQLEKGVNHLAESMNISIKDMNESGRGEMGSARGEYIQYVDGRE
                     EIVLNSRNSRTQNLTTSIHELAHKKLHNKNEHGSSYSTPIKEFQAEMTSFIVCHNYGI
                     DTSDFTIPYIANWTKNNEKIDPKNFRQIMQEVRETSIDFISTIDKKVLEEQQTAFNAL
                     KNSFVPNENKEQIQQKSDLEFHNFYDSTINDPDRTIHGSYLEAMVDYYRGKYGENSIE
                     LTHSNYDVTAKIGENYVTYYSDTKQFDVCDVYGVVFENPSENKNDRLLLDRSPENCDY
                     VEKFVVESLKACENHELKNDTLKLSEPLRSVVINEAVYNLSENANNYYNEKCWQEEIE
                     NQTNINDVKELNSCMTAEDFYESHKQEIENTITDFESVSGTNLRDTIDNESYKEVASV
                     VAYKLVATKVKYEMDEGTFKIPENSNLEKPTVKTAERSFEME"
     gene            complement(22644..23510)
                     /locus_tag="IWO38_001522"
     CDS             complement(22644..23510)
                     /locus_tag="IWO38_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313616.1"
                     /translation="MKKLRVIATIPMIFSFALLLSACGKEEAGAKSNEAHNISFIDSA
                     EQKDSRCWLIVETSSKNKTPIDKNEYVKAVIQTKDGKAKVFKTDMEQLTLEKVSKMSD
                     EEIIEQALNADKQKFNDKKKSDIDSYNATLDSVKTELENEEKQEFQNDQKIADFQDSI
                     PKIQADIEEIDGVQYSEPEWKMVSATIKMDNENKSPSMETVKLSYKYLDSPIWNLNSF
                     GEVSSEGELSYESTSMDLKLSIPVATTKVLDNFYSGYSNDELDQLLLTKVASKDNKVQ
                     LDSKGKNVSIVE"
     gene            complement(23685..23948)
                     /locus_tag="IWO38_001523"
     CDS             complement(23685..23948)
                     /locus_tag="IWO38_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313617.1"
                     /translation="MIDFRTVMDKLSNGDFRVLIELYLNVTSTVKEFLYSNPKTALVL
                     VAVLIVFAVISFIKKMIPLLIIGAVLILGLYFPEVLDQIKNIF"
     gene            complement(23950..24279)
                     /locus_tag="IWO38_001524"
     CDS             complement(23950..24279)
                     /locus_tag="IWO38_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313618.1"
                     /translation="MKNFDIVCSNTKNIYLRELLNSDSETIEDVKKIIVLFEKENMEL
                     ENWGLFEIPISGNYCFYNWKTEDDVAFANYFFDKNYFSPLYIDKHSNEQVASSIKEAI
                     KLERVRK"
     gene            complement(24301..25836)
                     /locus_tag="IWO38_001525"
     CDS             complement(24301..25836)
                     /locus_tag="IWO38_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313619.1"
                     /translation="MVGDDMTEHIFNRKSASIILKAKFETGKSKKNNHMVELQKFVDY
                     ISRQEAIRQDKLDHDYSEDELNELARIEKALEKIEQETDQPVIKDLREMDKYIDYMTR
                     KKAILENVDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNGSVMFQDVISFDNDFL
                     VREGYYNPETNELNENVLYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHVTAM
                     ERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRTNEFEKISKLRKEVPLEL
                     KQSVKDSLIQLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNY
                     MTKDNPKRKEYDRMTKEIDELYQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLL
                     KQSEEKNLKSNELKNLLDGKKLNVKFDREKPEFKTKYAKQSYDDFQKRLEERQLAREK
                     NEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMIEQEKMRQAREFEI
                     GMN"
     gene            complement(25903..26370)
                     /locus_tag="IWO38_001526"
     CDS             complement(25903..26370)
                     /locus_tag="IWO38_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313620.1"
                     /translation="MNYPYFKVSATEDVKEKFNKCFEVNKSVFQTKSNMFRYVVSNLP
                     MLTKANQNFRDPEKEELAKNLEEAKLKISELETIIELQKQQLENFNKKEDSTHKDYSE
                     QLNSIEESVYKLLINSGLASKELEEERAEPEQESSRLGEGTMFSSPSLRFTTD"
     gene            complement(26367..26663)
                     /locus_tag="IWO38_001527"
     CDS             complement(26367..26663)
                     /locus_tag="IWO38_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313621.1"
                     /translation="MDVSIRGIDPQYVKEIDKKVNKIQERTGKKFSRNDYFLAIIRQD
                     SEIDLIDYKKSEFDLVVEKLLLSNEQMVESISELTSVYERLFNFLISERSDLNK"
     gene            complement(27014..27244)
                     /locus_tag="IWO38_001528"
     CDS             complement(27014..27244)
                     /locus_tag="IWO38_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313622.1"
                     /translation="MCNSIVTIQEKAEEELMRIEAEYKEKRKAIKRKKNAELKKYKEE
                     QALKLGMMLCKYYDCYDINEVLKKANLNQFCE"
     gene            complement(27280..28176)
                     /locus_tag="IWO38_001529"
     CDS             complement(27280..28176)
                     /locus_tag="IWO38_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313623.1"
                     /translation="MNKKYSIHNKKEPNKKNKAQIDKVYKKTKGVKLYLGAILGFLAI
                     GMIIVFSPYVMGKSYDFETVKMSSAQNVTSSLNMYVDDMEINREAGLFKLVLRYQDDT
                     GTKSLSNIKSDYKLNFITNKNNEDVKTKVVKLSDEYTVIYYKNLPKDFGVISVTVNPR
                     YIYPELEPSNDLKEKEIKFYAVDKDIKSNAKLEVESTSDLQKDNFKYQIKAIENSIKD
                     EKKSIEKMNLANKVVQKDIDKANQNLEFQTLDEQTDTKNEISSMQTTIENNKSSIEES
                     ENKIKQFEEKIKQLEKSASRFE"
     gene            complement(28190..28840)
                     /locus_tag="IWO38_001530"
     CDS             complement(28190..28840)
                     /locus_tag="IWO38_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ0313624.1"
                     /translation="MIKRKNDNKWKYICGVLIIILVLGGLLSQLHTSNIQAKNKKLDQ
                     EVTQLEEQNSKLSKNISDLNANVEANLTNDDGSELNKQIAEVAKKFVELYPVYDIEKV
                     EEKKADLEKIATPSVADSIVPDDMITTSKKTLESKSTTQKGEAYSSDPTFKSKYKDSK
                     LYTNYVNANTINYFAEVNYKTESSSGDTENTVYMLFEVTNKDGQVLVDNYEIKYLK"
     gene            complement(28855..29991)
                     /locus_tag="IWO38_001531"
     CDS             complement(28855..29991)
                     /locus_tag="IWO38_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313625.1"
                     /translation="MEQNKNNNVASTLARQGMKQVSKKIGLALKIKIGVVIGLITLVG
                     LLVIGSLATLQGTQTATNTGGAEVIGGNKPLSPEVQKWQKTVEKYAEKYGIKELVPYA
                     LAIMQCESGGKLPDLMQSSESAGLPVNTLQYEESIDQGLKHLSNVYNIAKSYGMESNY
                     DGIVQAYNYGIAYMHYLGKNKKTHTIETAEAYSKNVVAPSLGNTTGQTYSYVNEVSIK
                     YGKTYLYLNGGNFYYAELVKQYIGGGSGNPANPSEFWGKMKTEMEKYQGWPYVWGGKN
                     PTTGFDCSGLTAYLYQFSAGITIDSYTVTQYEATEAVSIKEAKAGDLIFFRGTYGAPD
                     FISHVGIYVNETTMYDSNSSGIGYHNWQDSYWQSHSPEIRRVKK"
     gene            complement(30013..30387)
                     /locus_tag="IWO38_001532"
                     /pseudo
     CDS             complement(30013..30387)
                     /locus_tag="IWO38_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362769.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tra protein"
BASE COUNT         9059 a         5759 c         3701 g        12006 t
ORIGIN      
        1 gtctttattc tttgtcaaat cccataataa atatcccttt ggagtaggat aaacattcgt
       61 gttttctatc ttaatatatt ccctatttag aagagtgtct acaatattag aacgtgtagc
      121 gggcgttcct agccctaatt tcgccataat accggtttta cctaataatt taccttcagt
      181 caacctttta ggttccttag ttaatttatc aataaatcta atatctatat ttattgtctc
      241 ttgttcatga aatttaggta atgtttcctc tttatcatca gattcatcta aattagtatc
      301 tacttgcttc caacctaaag atataggtac attaccactt gtggtaaatt ctaagccgtt
      361 tacatctaat gtaacttttg tattttcgta cttgtaaggt tctgcaaaca ttagacacgc
      421 acgcctagta acgatctcat aaatattttt ttcatctaat gtcagatttg ctatatcagg
      481 aatatccatt gttggtatta gtgcataatg ctctaatact ttgtcattat ctacaaaccg
      541 ctttttaggc tctaaacaag gtgtatcaat actgatccct aaaacactct tatacacgtc
      601 taaattcgct tttaaatact caaattcatt tgtagttatc aattcactat cggttcttgg
      661 ataagataaa tatccacgat catatagact ttggataatt ttatctgttt ttttagcaga
      721 atatttccat tttttattag cgtattcttg aataccaccc aagctaaaca atttttgtgg
      781 ttctgtttgc tttttactaa cctcaatttt ttttatgatt ccagtactgg cggaatcaag
      841 gccgaatttt ttagtgtctt cttcaaattt ttctaattta aagtattctc gactattttt
      901 gaatataact tttggctctc tgtcaacagt atgacattca atcactttat acttctcaga
      961 cttaaaattt ttaattgata aatcattttc aacaaccaat gtattaacag gtgtctgaac
     1021 tctacctagt gataccaccc cagataagcc tttttgttgc aattttaatg tatacaattc
     1081 tgtataattc attcccacta aataatctgc aatttgtcgt gcatttgctt cttcaaagaa
     1141 ggaaatagtt tcctctgcat ttctaaggtt tttaaatccc tttattattt cacttttaac
     1201 caaagaattg atccacaacc gcttcatagg cttcttaaaa atagattgct tacacagctt
     1261 aaaaatatta taagcaatat tctctccttc acgatctgga tcggtggcta taataatttg
     1321 atcagcattt tctaagcacg acttgactac agtaaattgc tgactctttc cctttgaaac
     1381 ttcatacttc aactcatcca tttttggtaa aaaaggaaga ttagctaaat cccatttttc
     1441 tttgttaggt agccattcat atttttccgg cattgataat ccaactaaat gtcctatacc
     1501 ccaagttaca atcgtatctc caaatacctc actttgaaat tcaaacttcc ctccactttt
     1561 tggaattttc ttatcggcta acgcaaaagc gaatttctct gcttgatctg gtttttctgc
     1621 taaaattaca gttgccataa atactctctc cttcacttaa atttttcaat tgaacgctct
     1681 aatacacgtg ttaattttaa atttttaact tcaccatcat tcaattcatc aacacacatc
     1741 ttcttcatag attcaatggt agcaatataa gaaaatggtg gacaacaaat caacgaacca
     1801 acaacttcat taccatcttt cattgattta gccttataca aatgttgata cacataaggt
     1861 attaattcca taatccttca cctcccacta gattaaaaca atatctctta cacgagtaat
     1921 tgaagaattt tcagacttct gatacgttaa tctaattctt gaaccatcag ctaaatatct
     1981 tacactttcc ttatttacat caatcaatct ttttggaata ggttcacagc aataattagt
     2041 attatgccaa aaacaaaact ttaatttttc acctgaatca gtatctaata ataatattaa
     2101 attccctttt ctgccccaat aacgacatac agtttctccc tctactgtat ggattcctat
     2161 ttctgaagga aaatcatact ctgaacgtat taccgctata tcgcctttct taaaatctga
     2221 ttcattataa atcccatcaa tattcattaa tagccccctt ttttatttac ctgatatata
     2281 ttaattctaa caaaaaaaca gccaatatca aactatcaaa aaacagagta gctatctgtc
     2341 tacttgtagt caaaaaaaac aaattattct tttcttcttt ctctaatctt caatatattt
     2401 tttattccgt tttggggctg aaattttcaa atactcttta tactcttttt catactcaca
     2461 caagcaatca taaacctcat tcatattccc tcctgaagct agagacaaag catacaaaaa
     2521 agaaataggc aaatcagcta cctcacgctc tctagtaatc caagacgata gagttccttg
     2581 tttaaatcca tataactcac aaaacacctc aacagataaa cctagcttac cgataataaa
     2641 tacattgatc gggtgtgagt aaacatattt cttaattacc aaagaaaatc ttcctccttc
     2701 aaacataact gcatacgcag ttataagctg tattccgtac acgtggatta agggaaaaga
     2761 cacaagtaaa aatagttcta tacactccat acataaaaga gagtactaac tactaatcat
     2821 tagtatacgc taaaacagga ataggagtgg gcagaaacac tagatatgtt aattggcaat
     2881 ttagctattt aaaaatcaaa aaaataaata gaactctaac ctgtattagc tcctctaatt
     2941 ggcgcatgtt tgctcttttc tctttctttc agtatgttcg catggaacgg tactgttttg
     3001 tcttcataac gtcttaaaat cgtttctacg tggctatttg ctgttcctaa taattctaag
     3061 acaaatactt gtttatcttt tttattctga ataactgtat ttaatatttt agcttccaca
     3121 aatttgcttg ttgctatttt agtcgatcca taccgaccac gagacgttcg taatatcact
     3181 gtattcgccg ttctaagctc ttttaaaatc acctttaacg tagatacagg aatttgtgtt
     3241 tcttcagata tagttcccat agagacactt aaaaacaact catttttttt caatgatctt
     3301 tgttttcttt ctaagtaagc taataaatca gctttacttt cttcaaaatg actatatttt
     3361 cgttcttctc taggtttagc aaatttcacc caattagacg gatcgacacg attcccttta
     3421 cgtttaacag cttgtctatt tacatttatt gagtattttt caaactcggt aggtgtagca
     3481 taattttcga taatttcatt gacatatgaa cttttagcac ctttgtaatc gccacgataa
     3541 gcacttctta ttgttctctc aacttcagaa taggacaaat cattattgaa ttgaagttga
     3601 tccaattgat ccatacactc gccaatagat aagccacttg atttacatgc cagggctaag
     3661 gtaaaaatag ttttatttct atctccttca gcaatatccg tatgaattaa aaagtcgtac
     3721 catttagtat caatttgtcg tttagaccct ttcttgccag tagaattagg agctaataat
     3781 gacaagttag acctctttaa agcatattct ttttcttcaa agtcttttga ccatttcaaa
     3841 tacccttcaa acgtatctac gaaattaggt tcataatgta caatattttt tttagttgga
     3901 aatctaaaaa taccaaaatg attacagccc atatcgactt gtggtaaacg attttggata
     3961 gattttctga tattttctga tattctttta gcaactctca aagatttaaa atcatttgaa
     4021 ttagaaatat agcttgcctt atcaaatgaa tcagtttctt ggttatagtt ttgaatcaga
     4081 aaatagcaat gataaccttt tggcgtatct acaatcagtg taggcaataa cccttcttcc
     4141 aatagataaa gaactagtaa attcaaatcc gttttctttt ctcctactgg atagtcaata
     4201 tctgctacaa aagtattaat ctgaattaag ttagcttctt tgtgaccaaa aattgttttg
     4261 ttttcaccgc caccaagcca tgaatacacg ttaggagtcc agtgtgttaa tacatctgaa
     4321 tcttccaaga cacgatcaat agaacgatat ataagacctt tagtctttat catgtttgtt
     4381 ttagaagtaa atccggcaat agcaccaatc ttattgctat tctttttaaa agcaactaga
     4441 tttttgctat taaactgttt aattccattt ttaagaattt tagctaaaac gtccgcatac
     4501 tcgcaactat tcaacattgc actttctcct tgctatcaat tttgaatagc aaaaaagaca
     4561 tagggtaact ttagattccg taataaggag tcctaaaatt acactatgtc tttagagtaa
     4621 gcacttaaat gctaaacttt aaacgtgcaa aaattaaaat gagttggtag ctcatcttgg
     4681 tggacttcgt ggattgccaa atcgcttttc ttactacaga agttccatta aataagattg
     4741 gtagtcttat ttaattatct ttttcgctat ttttttcgtt acattcatac atagtagaga
     4801 gtctgtaact aacttttcta ctagaatttt ttagtgttcc tttggcttca gaactaatac
     4861 caacattata agctaataga attactttta acaagagttt ttttaaaaga tttttacccc
     4921 atcactttta cttgatttat aacataagta tttttatcaa taatttcatg aatgcaatat
     4981 ctgattttta aagtatttat ttttttcagt gttgtttcgt ctttgggaaa aggcagtttc
     5041 caaattagaa tacatcctga ttcagtatta aaaattattt cagtttctga aatatagtac
     5101 ttacttttaa caagtttaac attacctatt tttacgatct catctatttg atctcctacg
     5161 ttatacattt ttgtttctcc tttcttgata tatatattat atcaccttga ctacttgtag
     5221 tcaacttgga ataaaagaaa tagtctactt gtagtcatgt tttcaattgg aaaaaaattt
     5281 atttaaagat tcttttaagg aaacgcttaa acaatatttc ttagcttttt tacttgcaaa
     5341 ttcaggaaag tgactgtata atcatttata taaattatga aataaagact tcgtggattg
     5401 ccaaagattt ttcttactac gaacagtctt acgagtcaac gattgttggt agctttcgtt
     5461 ggctctttat ttttgccaca aaagactact tgtagtcttt tgaaatttca ttttcaggct
     5521 acttgtagtc aaaaaaaaag gaaagacatt ttcattgtct ttcctttttt ttacgatctt
     5581 tcgttagtct tctgtacggc ccaatcgttc ttgtactttc ttttgaaggc gttcttcttt
     5641 cagataatgt ttttctgtac actatgttag tgaacttcag caaattcatt ttctgccccc
     5701 tgttcactaa gctgttttgg attccaaact agttcataat ctccaaagaa atctttcaaa
     5761 accataggtt cattattaaa tattgctcct gtgtcgtaca ggctctcctg aagagttttg
     5821 ttataactac cagttaaaaa tacttcttta cttctataga tatatttatc ccacttacta
     5881 tccattttaa ataaaaacag ctcaacattt tttaaatttc cataaggtgt ttttaaacaa
     5941 tagctttcct tttgaatatc agaaaagact tgctttacaa tatgtttaaa cagcattttt
     6001 tcagctatat tactaactaa atataggagt aaaaatgcac caagtttagt tgtcgtatta
     6061 taataatttt gaaaatgaat ttcatgctca tacaggctaa gtagaaataa aactgtgccc
     6121 tgtctacaaa tgatttttat tcctttttga aaaggcttca aataattaat ataattgtca
     6181 tttgttgaca tcattatttt tcctacttgt aagataaata aagcaaaaat aaacaggctg
     6241 aaagcaagac ttcctaaaat atttaaggct tctttcacct ctaatgattc ttttaatcga
     6301 tcagttccgg cgaacagttt ataaaagcta taagcattgc tccatcctac tctagcaatg
     6361 aatagccaga ccagataacc taaacaacta aaactaattt tcagccctct cactatatct
     6421 aattgaaaaa tgagtctaaa ttccggctct ctatacatac ttttaaattt aacaatttct
     6481 tttttcaatc cgagcgataa tgactctttg cctatttttt tactcatttt actcccctta
     6541 aaccgcttca tcagcgattt ctttctcata aagtgaataa aaaacagaca atttaattgg
     6601 ctctaaatat tctaaagttg cttcagggat tcttttactg tctttaaccg caatataagc
     6661 tgaatcatta aaataaaaaa atttttctac attacttttt gcactaaaac catcataaaa
     6721 aattccaaaa agttctttac tacgtttttt tgaaaatgga ttcaccaatt caaaaacacg
     6781 ctttgaaatt ttttctagca attctttatt tgttctatag aatttactat ttttattgat
     6841 tccttttaaa tcttctcgac cactaatact atttttgaat gtagttactt tatttctact
     6901 ataaaattct ccatatattg atacttctaa agtaccatct tgccatacca ccaaatgatc
     6961 tatatctata ggatcactta atttactgat taaatcagca acaccatttt ctaaaatgta
     7021 atctaaatta ttatttaata ctctcgactt aacgtactct gatacgttag aattttttag
     7081 tgctttataa atagctgtca ttctttattc tccttcgtag ccttttgatc tacgaattta
     7141 actacccttt ttctatatac aaccctcctc attcaaaatt agatagtcaa gacgcttaac
     7201 aagactacat ttctattttg tatgatttac cgctataatt caacctcaat taaaatttat
     7261 ttcgtgccta acgatttaaa tattcagtta ctttctttat taaaaacagc aaactataaa
     7321 tgataataaa atcaaacact attaaaatca tacttgatat taaagcttta tttgttaatg
     7381 ttgtaatccc aatagtaatt actattaagt ttgtgattat aattctacca atattactat
     7441 tttcattctt atgaattagt aaatcataga catcaaatac gataatacaa actattaaga
     7501 acaatataaa tattacctta ttgttatata caaattcagt tgattttcct attaagtata
     7561 aaactaaata aattagctcc cacatattct cactctttaa ttcgaatttt catatacgca
     7621 attctcctgt gattcttcgt agacctgttt aatagaagct gatttaccta atcccattgc
     7681 acctaaaata tacttattat tggcgcatgt tttttggtat tccactgctt cacattcatt
     7741 ctccattttt tttactgaat caaatctttc gtgcttatta tccaactcca tgatacgcat
     7801 tatctcttgt gcatttgctt tactattaaa aatcataaat ggatatgttt ctccgtctac
     7861 ataaattccc caatcgcaga caacttcttt gatctctaat ttcataaaaa acaccttcct
     7921 gtagataatc aattatcact catgcttatc aattgattct tgaatggtat tgcaaggaaa
     7981 tgtattgaat ctgttttcgt catattctaa cgttgtatac tgtggcgtaa gagaactatt
     8041 atcttcaaca cttattgaat cgaatatgca acctaaaatt ctgaccagtc ataatttttt
     8101 tcttctgtac tttctgctgt aggatttcca aaaaatttca gtcgaaattc tttttcaaaa
     8161 tcattaaccc aattaacttg ttgattcact ctcgcatagc ctctttcttt tctatttctg
     8221 tcgttacttg actgccacaa aattaattct tttgtttaga agcttctttg tataattgta
     8281 caccttaaaa gtttgaaaca ggcttagaat ccttctctcg taacattgat ttctcaaaga
     8341 acttaaaatc aatcagattc acttattctt ttctttattc tatagactaa atttctattt
     8401 gttcctgttt tttctgcaat tcgcttaatc ggaacacctt ctccaagcat tcttactatt
     8461 tcattataga taaacctctt ttgtggattc tttgcagtag aagaatattc gtagggtttt
     8521 cctttataga ctcctttctt cttcgcaatt gctattcctt gtttttgttt tctcttaatt
     8581 tcctccctct cattttgagc cgtccaagaa aaaatgttaa ttactaagct tctaacaagt
     8641 ttttctaagt ttggatcacc aaattttgta tctaaaattg gtaaattcaa aaccattaag
     8701 cccataccat ttttatctat aaaagatact gtttctagta tttcatcaga atttcttcct
     8761 aagcgttcaa gcgactcaaa aacaagcgta tccccttctc tgacaaatga caacatttta
     8821 ttgaactctg gccgttctac tgtgttacgt ccagattttt tctcagcaaa tactttttcg
     8881 acaccaattt ttctaaattc ttcaagctgt ctttccaaat tttgatcatc tgaactgact
     8941 cgtgcatagc caactaacat atttaacacc tacaaataat tttactctag tttaattaac
     9001 taggaatttt ttcgtgagtt cagcagactc tctataccat caaataaaca ttcatagtat
     9061 tttatttttt cttttagctt tgaattttct tctttgagtt catttatcaa tttaacttgg
     9121 gatttttcag aaataaagcc ttcatcaaaa agcaaatctt ctacactaca attcgcaatg
     9181 tctgcaattt tttttagttt ggctttgttc ggtaagttac caccatgttc ccatgttgac
     9241 acagtgcttc taggagaatt agaaacctcg tacccaaatt cctgcatagt aagtcctaat
     9301 ttttttctaa tagaacgaat ttttctccct acctttttag ggtctggttt aacttctatg
     9361 tctcgataat cttcaaaatc ttttgagtaa tattttgtaa atatattttt tattatagga
     9421 ttcaataact catagtcttt atttagcgaa aacgtattac tatatctttc ttgaatgtct
     9481 ttaaagactt tgtgaggtat ttctggaaag tccttaatat atagctcata ccctatatcg
     9541 ataaggtatg aagttatata ttcttctaaa gttccccatt taatccagtc aattgttgaa
     9601 ttagtgatag ttgataataa tttcagcttt tgtggatttg gtaaattggt cccacgttcc
     9661 cagttggata tggtcgcacg tggtgaatta tctattttcc ttccaagttc ttccatagta
     9721 aaaccacatg aaaggcgtaa ttgtttgatt cgttttcctg cttctgaagg atttggcttc
     9781 atacaatcac ctctcttatg gaatttgggt tacttcaaat tcttttgagt aatattttgt
     9841 aaatatattt tttattatag aatccaataa ttcatagtct ttatctaacg aaaacgtatc
     9901 actatatttt tcttgaatgt ctttaaagac tttgtgaggt atctctggaa agtctttaat
     9961 atatagctca tatcctatac cgataaggta tgaagttata tactcttcta aagttcccca
    10021 tttaatccag tcagtggtgg tttctcctac tatagcaatc agctgtaact ttttctgaga
    10081 aggtatattt ctaccacttt cccaagtagc aattgtactt ttaggtgagt tgcttaaatg
    10141 agaaccaaat tgctccatag tcatacctaa tttttttctg atatatttga ttcttttact
    10201 tacgtctttt agattgggaa tcattttttc agctccttga tttactactt gttggatttc
    10261 atacaatctt tttcgtaagc tttctacctc atattttaat tcctcatttt cttgtttaag
    10321 aaggatattt tcggctgtca aactttcata cggctctact atattatcag tagaagaatc
    10381 gttgattaat gctactatcg gaacgtccaa tgctcttgca atagcttcca gtgtttttat
    10441 actgggatta cgcctgttgt tttctaaatc ccctatatat gatcgtgata gacccattaa
    10501 ctcagcaaaa tcagtttgtg tcatattttt actttttcta atatttttga tgttgtttcc
    10561 aatattaagg tttttgagtt caacatttgt ttcgtctaat cccatcaaat aacttacagg
    10621 aacatggaaa taatcagcaa ggatattcca tgtttcagta tttctaggat ttcgctcacc
    10681 acggtaatag ttcgtaagag ttgtatagtt aataccagtt tctgtactaa tttgtttaat
    10741 ggtcttttta ctggaatcaa tataaaattt tattctattc aaagtaacgc cccctatagt
    10801 aaattttatt ataggattca ataactcata gtctttatct agtgaaaacg tatcactata
    10861 tttttcttga atgtctttaa agactttgta aggtatctct ggaaagtctt taatatatag
    10921 ctcataacct gaatctataa ggtatgaagc tatgtattct tccaaagttc cccatttaat
    10981 ccagtctaca ctagtgtttc caatatttgc aattgttaaa agcctatttt tattgggtaa
    11041 agacacgcct ctaagccaat tattaacaga acttattggt gtgtcgccaa ttaaatcacc
    11101 gaatcgttct aatgtcagac ctttggattc gataattttt ctaatacgtt cactagttaa
    11161 cgatagatta ggtttcatag ctaagcctcc tcgtgagtaa agtaatcttt caatattttt
    11221 tgtgaatcaa cccattttat cgagaagtga tagttgacaa atagcattga gagatatttc
    11281 tcgcttatct ggatcatcaa tttttgaaat cacattaaga taaccggcga agctttcaat
    11341 tattctattg acttcctcaa ttgacagacg ttccaaagca tccaatgcta cttcagatag
    11401 aaaatacatt gattctgcta cgcttgaatc gacacccgtc ataagataaa gttcagaaac
    11461 tccgccaatt tctgcaatct ttttaattct ttctgagtta ggaaggtttt taccattttc
    11521 ccagttgctt ataacagctt tatcagctgt aggagagaat agtttgccga attcttctaa
    11581 tgtaatattc tctttttttc ttattttcct tatacgttgt ccaatcattt taatattttt
    11641 gttgtcatca gtcagtccta aatctggttt gacttctata tttcgataat cttcatcagg
    11701 agaatacatg tacttcacct cctgaaaaat attttgtgta ttgatttttt cggcagctgc
    11761 taatatcttt gtatcttctt cgtatgtgat tttttcaatc gtaagttcat ctagaagagc
    11821 ttcacggtag ttgttcagtg tagtctctcc gtactcttta agaatttttt cctctatatt
    11881 ttgtgtcaag cgataaacat aatcggaaaa atctccatac tttatccatt caacagaagt
    11941 accacctaat agtgcaattt tttcaagacg ctttctatta ggtaagttat ttcccttttc
    12001 ccaattgtta attgtgctag gactattggc atcaaccagt ttacctaagt ctgccatagt
    12061 atatccatgt ttttctcgta tttgctttat tcgattacca gcctcaaaaa gatttatttc
    12121 cattcgacac ctcctaatgt atatcatata ccgaaatcat ataaatctat ttcaatcaga
    12181 tttacaaaac ggtcattcat aaaaactagg gattttttcg tgagttcagc agactctcta
    12241 taccatcaaa taaacattca tagtatttta tcttctcttt taactttgaa ttttcttctt
    12301 tgagtccatc tatcaattca accgtttgag taatattttg taaatatatt ttttattata
    12361 gaatccaata attcatagtc tttatctaac gaaaacgtat cactatattt ttcttgaatg
    12421 tctttaaaga ctttgtgagg tatctctgga aagtctttaa tatatagctc atatcctata
    12481 ccgataaggt atgaagttat atattcttct aaagttcccc atttaatcca gtcgatagtt
    12541 ttgttactta attcagcaat tttaattaat ctttctttgt tgggaaggga gaatcctcgt
    12601 tcccaattat taatatttcc tttttgagca tttccaatta aatctgcaaa ttgttccata
    12661 gtcattccaa aattagaacg aattgattta attcttttac ctgtttcaac aggatttggt
    12721 ttcatacaat cacctctttt acagaatttg gtttcatagt atcttattcg ctctttgagt
    12781 tctaagttct ctttctccaa tttttctata taatattctt gatcctgttt cttgttcaaa
    12841 aaatgacctc caaatttcac attgtgttac accctagtgt tacacctcca aaccttatta
    12901 tactgtactt taccatatga tacaataggg tataccctat tgtgacactt acattctact
    12961 tttaatgtaa aaactcttga cctttatacg cataatgcgt ataataatat ttgtaaggag
    13021 gtagtcgcga atgccactta ccggtaaaaa aatgctcaaa ctagccaaga aaaatggttg
    13081 gaaagtggtg aggataactg gcagccatca tattgtagcc aaagaaggat tcccacccgt
    13141 tcctattcct gttcatagta atcaggattt aaaaaaaggg acagagcaga agttactaaa
    13201 ggacttaggg ctgaaatagc ccagtccttt agttcattgg attaactcga aaggagtggt
    13261 ggaaatgaaa aaaagttacc ctgtaatttt ttctgctgaa gaaaatggct actcagtagt
    13321 ttttcctgat tttgctggag caacacaagg agataatttt gaagaagcta tgtccaatgc
    13381 taaagaattt cttgatggga ttctagctta ctatattgat gaagatattt cagttccttc
    13441 tccaacagat attcatcaac taaataattt gggcaataac aaaattgttt ctcttattca
    13501 aagtgatcct tctccattta tccaaaaaaa gaccgtacgc aaaaacgtca cagtcccaga
    13561 atggttagta gctagagggg aaaaagataa aataaatttt tctaaagttt tgacagatgc
    13621 gttactaaaa atatacgagt aaacgtaaat atattaataa caatttagtt gcctaatgac
    13681 atagatagcg gaattaaaag taaaatagta ctcatttatt gcaggttccc ttagaatcaa
    13741 tacaataaat gagtactatt tagactgttt aactactagt aagatatagc gatcatcagt
    13801 ttcagggtta tcccttggtg accatagttt tgcgtatttt tcaactcgtt gccaaatcaa
    13861 cgattctatt tttttatttc tacttaactc caaaacggag taccttcttt ttcttcgtta
    13921 gctcctattt aagagccatg tcgttttata taactctaaa acagaaacta ctgatcaatt
    13981 caataaagcc ttatttgaga actgtgttgt tttacatggt tttaaaacct gatttcccgc
    14041 aaacggttta tccgtaacat ttgagaactg tgttgtttta catggttcta aaacagatta
    14101 gggaagatca atctatcaat ctatatttga gaactgtgtt gttttacatg gttctaaaac
    14161 ctcaacaata ttcgtccttc cgtctggtta cacaacttga cagagctaac ccttcagtga
    14221 gtgagtgtcc cctcactatt tcgttgccaa atcaacgatt tcttattcta ctcactttta
    14281 acttctattc catatctttt ccctttattt attatttgtt ctttcaactg ataataagcc
    14341 cattccccat aatttttatc tggttcagaa atacagatta tttgcgtctt attgcggata
    14401 gctgtttgaa taatttttgc cgaacattga ttattatagg ttttaacaaa attgctttct
    14461 cttttcttta tggcttccca tctatcttga tctaattttc tttttcgacc atgaccgcct
    14521 ttagaaaaaa ctcctcggct ttgctccctt gatttcattg ctttaatttt atttttgaag
    14581 ttacttatat atttgctgtc accaaaatca atcacagatt gattgttaac taaacattta
    14641 ccgctacaca aatcagtaaa cattatggtt aattcagctc cttctttgaa atctagctta
    14701 tgataatctt tttcaaaaga aattgttaaa ttcaaaaata aatttttccc ctcaaagatt
    14761 agattactct ggtttaactt gtattgaccg tccataatgc gttctaaagt taaagcgcta
    14821 tctctggctt ttcttggttt tctacctaaa tttactttaa acctaaatcc tttaggtaaa
    14881 gagatataaa acttgtcagc tttgtgaatc ttaatatccc gacctctgaa cggtaacaat
    14941 ccatatttat ttttatttct atagattcta ggtgttcttt gcccagtaac aattccaaca
    15001 aaatcattgc taaaatcctt actagctttc attttagctg aatctataaa atccgaataa
    15061 tcaaagtcca atttttttgc tatatcacgt attctggttt gactttttaa acctatagct
    15121 tgtgtcaaaa cgtctctggc ttctttgttt ttcgcaattc gtagtttaga taattccctt
    15181 ttcgttttat ctaatttctt ttgatcgatc acttcattac ttagtaaatt atcaattcgc
    15241 tcttcaagac ttagctctct tttaatatac tcatgatcca attcttttat agaattttcg
    15301 atcaattctt caaacatcag ctgtttatat accatattca gcgcttttac atattctcta
    15361 ctatttttcc aaaaaaaacc aaaaaacgaa tctctttcct cttgtgatcc atcaacatat
    15421 acttctattt ttttagtcat agttattatt gtcaatttta gcacctcatt attatatatc
    15481 tggtatataa taatagtaac atatttccta gactataatc aatgatcaat tgtctacttg
    15541 tagtaattat attaaaaaca aaaactaaaa ataatatatt attccttctg actacatata
    15601 gtcactttat ccaaaaaata agaagtgctt tcgcactcct tattaaattt atttactatt
    15661 aattgattca gctacaactt cttttattca acttcaaaat cagttttttg gatatttcta
    15721 tctctattta attccatact taatagctca ctatcagttt ttactaaaag ctcacctaca
    15781 ctattaatat atgccaattt acaagaattt cttcttttat cttgacctac taattcatct
    15841 tgagataccc ctgtagaaaa actaattgat agaatccgtt ccttgatttc atttttatgg
    15901 ttcctataaa aatcatcgac ttgcgcttct tttgaaaacc gatcactaat aaaatcatca
    15961 cttgctgaat atattctttc aagggtttct tttggattca tctcttcttg agatatatcc
    16021 atcataattt gtttgcttat aggagtttca gcatttttct cttgaatagc tttgggaacc
    16081 caagcatatt cttgcgtatt attggctaca actgtatgat tttctttaac ataaatataa
    16141 gaaaactcat ctgaattatc atagatactt acattatcaa aatatttctc taactctgct
    16201 aagtttctta aagaaaattc gtatcttttt cttattaaat cttcaggtac accgtggcca
    16261 cctttagcaa cacggctttt aaccctttca atcgctcttt ctggtgaatc tagtcctata
    16321 tagattaaaa acgattcata acctaatttt ttagcattta gaagcctctt tttatcggga
    16381 tatggattac cgccaaaagt cgtttcatgg tgaaaagatt ttccttcttt aaaatgacct
    16441 tcgatctctc tcagaacttc acgcatactt ttgtttatat ctgattgttt attccaattt
    16501 cctccaaaac gcctcaaaat ttcatctgca ttaattctat cagtatcaag ttcttttctt
    16561 tttgaatttt gaatttgata aatcgtagat ttaccggcgc cattaacgcc cccaataact
    16621 acataatatg gtttatcatt ttctaaaatc atattttaaa atctttctga ttaattactt
    16681 ttttttgatc tactcgtgtc acataactag agagtacaag gtagaaagtt ctctcttcct
    16741 cactttctgc attttttaag gcttcgtgaa aatcatctac agtcatattc aaaacatcag
    16801 gatacatttt ctttaattct tttacagcct cggtcatatc tctcattccc ttcttattag
    16861 tctttattaa agttctctgc ttcagcttct agtctatctt tcaagtcttt taaaacaacc
    16921 gtaagtacat ggtcttcacc atttttttca attgattgtt ttaccgcttc atctaactta
    16981 gctaaatagt cttctataat tcctaaattg tttacttcaa taagtcggaa gtccggtaaa
    17041 tttttcatcc gaataccacc tttaggctgt tcctttacaa ataaagattt gtaatcatta
    17101 tattcattcc cattaataac caattctttc ataggctcta agtggataaa tttacgtgaa
    17161 gcatagtctt tcataataat ctctcccagt agaaaatctt cttttttcta ttatatcacg
    17221 ttctcaaagc gttgagttat tggattgttt aaaccatcaa gacacatcat gctgcatttt
    17281 ttcatcacta ccggtgcttt tccgtttgct ttaactaccg ctacattagc taacaaacta
    17341 attacttctc cctcgataag ctctccgctc ccaataacaa cgccctgtac ttccatacct
    17401 actttaaacg tttctttttt atttaccatt attccgctct ccttccacga ctacttgtag
    17461 tcatttggat attttttagg ggaacggttt tccgttccct ttcatcaaat agaatccttc
    17521 atcatttcaa caaataatta actttcagtg taaaaaaatt cttcgtaatt accatcatga
    17581 aaaatatccc atctttgttc gtcattctca ctgaagtagg taaaataata tctgacagta
    17641 tctaacttat ttaattgctc tctggttttg taaatacaat ccaagtgatc ttgcattcga
    17701 tctgaccaaa acattcttgt ctgctccatc tattatctct cctttttatt aaatcaaggg
    17761 tttgagtctt tgactacttg tagtctatgg ctatatcata caggaatgac tacttgtagt
    17821 caatagtttt ctttagttta attaactgtc tattcgtagc caaataatgt aaaaaaacgc
    17881 ctagtttaca ctagacgttt aaacaattca ataattcttc gttatacccc attaagggtt
    17941 gaactgcttt aatacttact tgatcgtctt ctctaacggc ttctatgctt accccttttg
    18001 tatattggcc atctttctta tccgtccaaa tttgataata agtaagccca tatcgttcaa
    18061 taatctcaaa gagaatagtt attaattttt tttgtttaaa ttttggtaat tcaatacgca
    18121 atgatttttc ttgaatatcg aactctggat ttttattttt aagttgttct tcaataatca
    18181 tatagtctgt agacgtctta tctaaatcaa ttaatgaatc tgataagatt gctattgtat
    18241 cgttcataac acatcaacta ctttcttctt gttattccat ttcatataca ggagtattaa
    18301 gagataattt atgtgtgaag ttcacgattt ttgatacatc ttctaacttg aagcgtttaa
    18361 aagcttcttt tcctcttgtt aaattatcat tccaatcaaa tccctctcgc aaaggtacat
    18421 cgacttttac ttcactgatt cctatccctt ctcttccaat tcctttttct ttaaattcag
    18481 tttcaataaa agtacgtccg gctttatcat tatcaacaca aaggactgtt tctgaaggca
    18541 atgatttaaa cttattataa tgctctgtaa ccccatgcaa tacattacct acctttaatc
    18601 cattcatgct aacaaacgta gcattttcgg ctttttctgg cttttgtgta atgtacgaca
    18661 tcaaatctat ttcactttca aaaaaataca ttttatctgg ttgaccaaaa gttacgttaa
    18721 atcctgaata ctctttagag ttttcttgta tttttttcca tgtaacatcc ttttgtttgc
    18781 cttcatgaaa aaattttcta gttccttgtt ccgtacaccc aacgatttct tgatcttttt
    18841 tccataagaa caaaacattt ccttttttat cttgtttaat tagtccttta gataaaaaat
    18901 cttccactaa agctgtatcg attcttctct catttactaa ataagacttt gcaacagata
    18961 ccggcacttc attttctttc tgatattggt acggagcttt tttcactttt gtattgaacg
    19021 aatgaacttc ataatcgcta tcttcaagtt tctgttttgc ttctttaaat gaacaatcat
    19081 agtaggcttt gataaaatca aaaatatctc cccctaaatc ctttgagttc catttaaaaa
    19141 ggtttttgcg atcactgatt acacaagaat catgatcttc taatctatag tattcccttg
    19201 aatcttgctt taaacctatc ccattttgtt tgcaaaaatc aacgatactt actttttttg
    19261 cctttttcaa atccaaacaa aaaccccctt tactagacca tcactagtaa aattacgata
    19321 aggtacactt gtatttttca taataatcct ctgtacagaa aataaagttt agcccatccc
    19381 taagagataa ctcccaaaag cctatcttct tgctcttttt ttctctaatt atcttttttc
    19441 taatactttt tccgtacttc aaagccattt taaaagcgat ttgtacatct tcacaaatta
    19501 caaaatgact attattaatt tgacgatatt caccttgcgt ttctaaaaaa tttctatttt
    19561 cttgttctat cattcattta tctactccat ttcaaatttt gttgtatttt caaaattagt
    19621 caatttcaaa atcttattct ccatttcttc agaaacaggt ggtatttcaa acgtatcatt
    19681 ttcaatttca ttataaattt ctctcattgc accgttgtaa gccatgatag acgtagcttt
    19741 tttgtattcc gtagaagaag tatctactgg aaaacctagc agttcttctt tatcgatctc
    19801 taacttttcg gataaatttg tcaatttcgt ttcaatttct tctttatgac tatcataaaa
    19861 ttcattaata tcactatcta atgctaatct ggaaactggt tcttccatgt tttctaagta
    19921 ttcaatgcgc tcttgccaag cttgtttgtc ataataatta gttgtttcta gctcttgctc
    19981 aaaatctttt atcacaactt ctcgaatggg aacttccgaa accttatgcc caacaaattt
    20041 atctgtcatg tcctcactac tttctaagct attccgttcc tcgtttaaat cgtgaatagt
    20101 ttcaatcttt actttatcag gctgtaacac tacgataact acatcctttt gtcttaactg
    20161 ttcttccgca ataatatgtg tatatttccc aacgtcataa aatgccttag cagtagcttc
    20221 atgatctcca attgtatttc caatgtcgtt aatcaaatct acgtcatttt cattctcact
    20281 aagagaagca atcacttctc tttttacaac tttttctccg taataactaa catcattgta
    20341 atcatttaaa atatcattac tttcttgtaa tttatctatt actttagaaa gctcttcatc
    20401 agtaatagtg cgttcattca tagaaatcgg cttctctaat tcaacattta cttcatatac
    20461 gaaaccatta tttccgttac taccctgttc agcgtacatt gtagccattt ctttattagg
    20521 tgttaagtac acaccaaatc cattagctgt tccgtttaaa cctttatgat ctaaactaaa
    20581 ttcatcaaat ttattcatac ttccatgaaa caggcgataa gttccgcctg tttcataatt
    20641 ttgttcatta ctcataattt tttcactcca tttcaaaaga acgttctgct gtttttacag
    20701 tcggcttttc caagttagaa ttttctggta tcttgaaagt tccttcatcc atttcatatt
    20761 ttactttagt tgcaaccaat ttataagcta ccactgaagc cacctcttta tatgactcat
    20821 tgtctattgt atctcttaaa tttgttcctg acacagattc aaaatctgta atcgtatttt
    20881 cgatttcttg tttatgagat tcgtaaaaat cttccgcagt catacaagag tttaattctt
    20941 ttacatcatt aatatttgtt tggttttcaa tttcttcttg ccaacatttt tcattatagt
    21001 aattgttagc attttcagat agattataaa ccgcttcatt tataacaact gatcttagcg
    21061 gttcgcttaa ttttaacgta tcatttttta gttcatgatt ctcgcaagct ttcaaacttt
    21121 ctacaacaaa cttttcaaca taatcacaat tttcaggtga tcgatctaat aataaacgat
    21181 catttttatt ttcacttgga ttctcaaaaa ctacaccata aacatcacat acatcaaatt
    21241 gtttcgtatc tgaataatat gtcacatagt tttctcctat tttagctgta acatcataat
    21301 tgctatgagt aagctcaata gaattttctc catactttcc tctataataa tccaccatcg
    21361 cttctaaata actaccgtgg atcgttctat ccggatcgtt gattgtacta tcgtagaaat
    21421 tatgaaattc taaatctgat ttttgttgta tctgctcttt attctcatta ggaacaaagg
    21481 aattttttag tgcattaaat gctgtttgct gttcttccaa gactttctta tcaatcgtac
    21541 ttataaagtc aatagacgtt tctctaactt cttgcattat ctgtctaaag ttttttggat
    21601 caattttttc attattttta gtccaatttg caatataagg aatagtaaag tctgacgtat
    21661 caataccgta attgtgacaa acaataaatg aagtcatttc cgcttgaaac tctttaattg
    21721 gtgttgagta ggaagaaccg tgttcattct tattgtgaag ctttttgtga gccaattcgt
    21781 gaatactggt agtaagattt tgtgttctgc tatttcttga atttaataca atttcttccc
    21841 taccatcaac atattgaata tattcacctc tagcgcttcc catttcacct ctaccagact
    21901 cgttcatatc ttttatagat atattcatag attcggccaa atgatttaca cctttttcta
    21961 attgactaac acttaattca ccatcaatat taaaattcca taccctgtta ggaaatattt
    22021 ttggcaaatc agcttcagtt gcattagttt gggaaatatc aaatacgtaa cccttcttaa
    22081 aatgcttgat ctttttgctt cttaattctc catttttgat tttttctttt tgctcgcttg
    22141 ttgcttcttt taacgtcttt tcttcaccat tttcatcaat aaaaaccgtt accggagcat
    22201 gcgcataaat gcctatccct ttttctttcg ctttaacttg aaatcctagt tttttccaac
    22261 catcataact tgctacagca attgctcctt caaatccctc atcgatcaac gataggttta
    22321 aaggactgta attgtgtatt ttagacatga acttggcaaa ctcgatcata tcttcagggt
    22381 tagactgata ttgctcgatt ttgctgttgg aagccttaat taaatcgtct agctcttttt
    22441 gtttttgctc tttcgttttc ggcttccagt ttcctttttt ataggccatc ttctatctcc
    22501 tttctttttt aggtaagtta aattacctaa ttaaattata ccagatagat aaaaactctt
    22561 ctatctaatt tcactgactt aatgcaaata aaaaagagat aagatagtta tagtaaccat
    22621 cttatccctt cttattttac catctactca actatactta cgtttttacc tttactatct
    22681 aattgaactt tgttatcttt agaagctact ttagtaagca aaagttgatc caattcatca
    22741 ttagaatatc ctgaataaaa attatctaaa actttcgttg tggcaactgg aattgataac
    22801 ttcaaatcca tactagtaga ttcataagat aattctcctt cagacgaaac ttcaccaaaa
    22861 ctatttaaat tccaaattgg actatctaaa tatttataag ataatttaac ggtttccatt
    22921 gaaggtgatt tgttttcatt atccatttta attgttgctg ataccatttt ccactccggc
    22981 tctgaatatt gaacaccatc gatctcttct atatccgcct gtattttagg tatactatct
    23041 tgaaaatcag caattttttg gtcattttga aactcttgtt tttcctcatt ttctaattct
    23101 gtttttacag aatctaaagt ggcattatag ctatcaatat cgcttttttt cttatcatta
    23161 aacttttgtt tatcagcgtt caatgcttgt tctattattt cttcatcact cattttagaa
    23221 actttttcca atgtaagctg ttccatatcc gttttaaata cttttgcttt gccatcttta
    23281 gtttggatca ccgcttttac atattcattt ttatcaatag gcgttttatt tttactactt
    23341 gtttcgacta tcaaccaaca acgtgagtct ttttgttctg cactatctat gaatgagata
    23401 ttatgtgctt catttgactt agcaccagct tcttctttgc cacatgcaga taataatagc
    23461 gcaaaactaa aaatcatagg tattgttgca ataactctta actttttcat ttataagcac
    23521 ctctttttta tcggtctgat atataatttt atattatcat atttttattc caatacacag
    23581 atactcttat ataatgtctt ttcatgtagt ttatcaaatt tctatttcaa accgcttaaa
    23641 acaaatatca aagcccaaaa ttttctactg gaaactttta gagcctaaaa tatatttttt
    23701 atttgatcca aaacttcagg gaaatacaaa cctaatatca acacagcacc tataattaat
    23761 agcggaatca ttttcttaat aaaagagatc acagcaaaaa ctatcagtac tgcaactaat
    23821 actaaagctg ttttgggatt agaatataaa aactctttga ctgtagacgt tacatttaag
    23881 tatagttcaa tcaatacgcg aaaatctcca ttacttagtt tatccataac cgttctaaaa
    23941 tcgatcatat catttcctca ctctttccaa ttttattgct tcttttattg aagaagcaac
    24001 ttgttcatta ctatgcttat ctatatataa tggggaaaag taatttttat caaaaaagta
    24061 atttgcaaaa gctacatcgt cttctgtctt ccaattataa aaacagtaat tcccactaat
    24121 cggtatttca aaaagccccc aattctctag ctccatgttt tctttttcaa acaacactat
    24181 gatttttttt acatcttcta tagtttccga atcactgtta agtagctcac gtaaatatat
    24241 attttttgta ttactgcaca ctatatcgaa gtttttcata atttctattc tccgtttcta
    24301 ctaattcatt cctatttcaa attcacgtgc ttgtctcatt ttctcttgct ctatcattcg
    24361 ttgcgctact tcataatcat ttttcgcacg atacatattt aaatcatcat tgattactct
    24421 atcaattttt ctaataagct ttttatcttt aaatttttca gaaatcttct tgaagtggac
    24481 ttcatttttt tcacgtgcta attgtcgttc ttctaatctt ttttggaaat catcataact
    24541 ttgtttagca tatttcgttt taaattctgg tttttctcta tcaaacttaa cgtttaattt
    24601 ttttccgtct aatagatttt taagttcatt acttttcaaa tttttttctt cagattgctt
    24661 taaaagtttg atttgtccta ctacagcatt acccattcga gcattaaaat ctttttttct
    24721 atttaaataa tattcttgtg gattgtagcg tttaccgtag gttgtttgat atagctcatc
    24781 aatttcttta gtcatacgat catattcttt tctttttgga ttatcctttg tcatataatt
    24841 tgtgacttca tcaatctttt cttttgtttc ttcaggaagc tcattgtagc cacgtgttgt
    24901 actaggtatt tcttttttta attcctttag gtactttact aattctttgt catatcttga
    24961 attattttct acatataatt gaatcaaaga atcttttaca gactgtttaa gttctaaagg
    25021 aacttctttt ctcaacttag aaattttttc aaactcgttc gttctatcta atattttgct
    25081 accgaattta aaaatcatat cgtctaacgt agactgtttt ctcttgcccc tagcttgtaa
    25141 aacaccatca tattccatta tttcacgagt atttttacgc tccatagctg ttacatgaat
    25201 atggatatgt tcagtatttc tgtgaatcgt tgcaaaccaa aaaggatcaa ctagttcttc
    25261 cttttcttgc atttttccca tcatagactt tgttgcttca tatagaacat tttcatttaa
    25321 ttcatttgtt tctggattat aatatccttc ccttactaaa aaatcattat cgaatgaaat
    25381 tacatcttgg aacatcaccg atccattatt ttttgcttct atgaccgatt cttttatttt
    25441 tgaaatgtct tttttagtga tatatctttt tgtattgcta aatgctccgt ttactatttc
    25501 attatctaca ttttctaaga tagctttttt acgtgtcata taatcaatat atttatccat
    25561 ttcacgtaaa tctttaatca ctggttgatc tgtttcctgt tctatttttt ccaaagcttt
    25621 ttctattcta gctaattcat ttagttcatc ttctgaataa tcatgatcta gtttatcttg
    25681 cctaatagcc tcttgtctgg aaatataatc tacaaatttt tgaagttcta ccatgtgatt
    25741 attctttttg ctttttccag tttcaaattt tgctttaaga ataatgctcg cactttttct
    25801 gttaaagata tgttcagtca tatcatcacc caccattttc aaaaattttt tctacaggcg
    25861 gttttgtact taatgctaag ttgcattaga attttttagt gttcaatccg ttgtaaatct
    25921 taatgacggt gaactaaaca ttgttccctc tcccaaacga ctactttctt gttctggttc
    25981 agcacgctct tcttctaatt ctttactagc taatccacta ttaattaata gtttatagac
    26041 agactcttct atactattta attgttcact ataatcttta tgtgtactgt cttctttttt
    26101 attaaagttt tcaagttgct gtttttgcaa ttctattatc gtttcaagtt cgctaatttt
    26161 taattttgct tcttctaagt ttttagctaa ctcttctttt tctggatcac ggaaattttg
    26221 attggctttt gttaacattg gtaaatttga aacaacataa cggaacatat tggattttgt
    26281 ttgaaatact gacttgttta cttcaaagca cttattgaat ttttctttta catcttctgt
    26341 tgctgatact ttgaaataag ggtagttcat ttatttaaat cactcctttc gctaattaaa
    26401 aaattgaata agcgttcata aactgaagtt aactcgctaa tactttctac catttgttca
    26461 tttgacaata aaagtttttc aactactaaa tcaaattctg actttttata gtcaatcaaa
    26521 tcgatctcgc tatcttggcg aatgattgct aaaaaatagt cattcctact aaactttttt
    26581 ccggtacgtt cttgaatttt attaactttt ttatcaatct cttttacata ttgtggatca
    26641 attcctctga ttgaaacatc catacacaca cctctttcaa gctgttttta tctatctggt
    26701 atatattatt atatcaaagt ccaaaggaaa attaaagatt taaagacaag accaaggaat
    26761 taatacaaag agcataaggg aaaactgata gcaccttcct aaaggaaggt ggctaagttg
    26821 gctgtgccaa ctggttttct ttcaaaatag cttcatattt tttgctatca caaaaaaatc
    26881 cattttcgac ctattttcgg tcataatata gtacctactt ttggtcatag tttcgtccgt
    26941 agttttggtc atagtaacta aagcttttac gcccaaaaaa agagtggcaa gttttacctt
    27001 gccactctct tttctattca caaaattggt ttaaattcgc tttttttagc acttcgttta
    27061 tatcatagca atcataatat ttacacaaca tcattcctaa ttttaacgct tgttcctctt
    27121 tatatttttt aagctctgca ttttttttgc gcttaatcgc ttttctcttt tctttatatt
    27181 ccgcttcaat tctcattaat tcttcttcag ctttctcttg aatagtgaca atagaattac
    27241 acattctctc taaactccct taaaataatt taaacacgat cactcaaatc ggcttgcaga
    27301 cttttctagt tgctttattt tttcctcaaa ttgtttgatt ttattttcac tttcctctat
    27361 tgaagattta ttattttcaa tagtcgtctg catagaactg atttcatttt ttgtatctgt
    27421 ttgttcgtct agggtttgaa attctaagtt ttgattagct ttatcaatat ctttttgcac
    27481 tactttattt gctaaattca ttttttcaat tgattttttc tcatctttta tagaattttc
    27541 tatagcttta atctgatatt taaaattatc tttttgcaaa tcagaagtac tttcaacttc
    27601 caatttagca tttgatttaa tatctttgtc tactgcatag aattttattt ccttttcttt
    27661 taagtcattt gaaggttcta attcaggata aatatatcta gggttaactg tcacagaaat
    27721 tacaccaaaa tctttaggca gatttttata ataaattacc gtgtattcat ctgacaattt
    27781 tacaactttt gtcttaacat cttcattatt tttgttcgtt ataaaattta atttatagtc
    27841 acttttaata ttacttagtg atttagtacc tgtatcatct tgataccgca atactaattt
    27901 aaacagtccg gcttccctat ttatttccat atcatcaaca tacatattca aagaagacgt
    27961 tacattttgg gctgaactca tttttacagt ttcaaaatca taacttttcc ccatcacata
    28021 aggagaaaaa acgattatca ttcctattgc taaaaatcca agtatagcac ctaaatataa
    28081 ttttactcct ttcgtttttt tatatacttt gtcaatttgt gccttatttt ttttatttgg
    28141 ttccttctta ttatgaatgg aatatttctt attcatatac ttcacctcac tattttaagt
    28201 acttgatttc ataattatct accaacactt ggccgtcttt attggttact tcaaaaagca
    28261 tatagactgt attttctgta tctccactag aagattctgt tttataattt acttcagcaa
    28321 aataattaat agtattggcg ttaacataat tcgtatataa ttttgaatct ttatatttac
    28381 ttttgaatgt tggatcactt gaatatgctt ctcctttttg agtagtagat ttactttcta
    28441 aagtcttttt actcgttgta atcatgtcgt ccggcacgat agaatcagct acagaaggag
    28501 tagctatttt ctctaaatca gctttctttt cttctacttt ttcaatatca tataccggat
    28561 atagctcaac aaatttctta gcaacttcag caatttgttt atttaattca ctgccatcat
    28621 cattagttaa gttcgcttct acatttgcat ttagatcact aatattttta cttaacttgc
    28681 tattttgttc ttctagttgt gtaacttctt gatccaattt tttatttttt gcttgaatgt
    28741 tacttgtatg aagttgagat aacaatccac ctaaaactaa aataataatt aaaacaccac
    28801 aaatatattt ccatttatta tcattttttc tcttaatcat ctattttctt cctcctattt
    28861 tttgactcta cgaatctctg gactatgtga ttgccaatat gaatcttgcc aattgtgata
    28921 accaattccg cttgaattac tgtcatacat agttgtttca ttgacataaa tacctacgtg
    28981 agaaatgaaa tcaggcgctc catatgttcc tctaaagaaa atcagatcac cagcttttgc
    29041 ttctttaatt gatactgctt cagttgcttc atactgcgtt actgtataac tatctattgt
    29101 aattccggcg ctaaattgat aaagataagc tgttaatccg ctacaatcaa aacctgtcgt
    29161 tggattttta ccgccccaaa cataaggcca accttgatat ttttccattt ctgttttcat
    29221 ttttccccaa aattctgaag gattagccgg attaccacta cccccaccga tatactgttt
    29281 aactaactct gcatagtaaa aattaccacc atttaaatac aaatatgttt ttccatattt
    29341 aattgatact tcatttacat atgaataagt ctgtcctgta gtatttccta aacttggagc
    29401 aacaacattt ttagagtagg cttcagccgt ttcgatagta tgtgtttttt tgttttttcc
    29461 taagtagtgc atgtaagcta ttccgtaatt gtaagcttga actattccat catagttact
    29521 ttccatgcca taacttttag caatgttata cacattactc aaatgtttta gcccttgatc
    29581 gatactttct tcatattgaa gtgtgtttac aggcaatccg gctgattcgc tactttgcat
    29641 taaatccggc aatttacctc cggattcaca ttgcataatt gctaaagcat aaggtactaa
    29701 ttctttaatt ccgtattttt cagcatattt ttctaccgtt ttttgccatt tttgaacttc
    29761 aggtgacaac ggtttgttgc caccaatgac ttctgcacct cctgtattag ttgcagtttg
    29821 tgttccttgt aatgtagcta aagaacctat aactaaaagg ccaaccaatg ttatcagtcc
    29881 tataactacc cctattttta tttttaaagc aagacctatt ttctttgata cttgcttcat
    29941 cccttgacga gcgagtgttg aagcaacatt attatttttg ttctgctcca ttaaattcac
    30001 ctccgatttc tattattgac caccatcgaa tcgttcaatt tgctctggat atggatcaaa
    30061 attaatcata taggtgtctt cactggaagt ttgaactaca gcttgtccta cttctaattc
    30121 tggtaaaagc ataatttctg tatcagtaat tgtatttcct aaaacttttt ttatgtcttc
    30181 caacaatgca gaatctaaat taaagaagca tttaaaagta catagattaa aaatgtttct
    30241 aatcacatct gctacttctg atccagaatc tttagggata atttcttcag gcgtttgagt
    30301 tgcaaaataa acacctgcac gcatttttcg catttctcgc tcaaaactta gtacatattc
    30361 acaagcatac ttgttatttg aattgataaa tactgatagg taagagtttg atattatctc
    30421 cgtcttttcc cccagtccac accgtacgtg cgactttcac cgcatacggc gttccctcgt
    30481 ctataaaaga cattctgttt tcccgtccta caaatattta ttaag
//
LOCUS       DACECY010000032        29570 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.033, whole genome shotgun sequence.
ACCESSION   DACECY010000032 DACECY010000000
VERSION     DACECY010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.033"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(47..1342)
                     /gene="purB"
                     /locus_tag="IWO38_001533"
     CDS             complement(47..1342)
                     /gene="purB"
                     /locus_tag="IWO38_001533"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ0313626.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1355..2488)
                     /gene="purK"
                     /locus_tag="IWO38_001534"
     CDS             complement(1355..2488)
                     /gene="purK"
                     /locus_tag="IWO38_001534"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ0313627.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2472..2960)
                     /gene="purE"
                     /locus_tag="IWO38_001535"
     CDS             complement(2472..2960)
                     /gene="purE"
                     /locus_tag="IWO38_001535"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ0313628.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3376..4683)
                     /locus_tag="IWO38_001536"
     CDS             complement(3376..4683)
                     /locus_tag="IWO38_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ0313629.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4686..5267)
                     /locus_tag="IWO38_001537"
     CDS             complement(4686..5267)
                     /locus_tag="IWO38_001537"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ0313630.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5383..5480)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5839..7131)
                     /locus_tag="IWO38_001538"
     CDS             complement(5839..7131)
                     /locus_tag="IWO38_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0313631.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7128..7751)
                     /locus_tag="IWO38_001539"
     CDS             complement(7128..7751)
                     /locus_tag="IWO38_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313632.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7754..8590)
                     /locus_tag="IWO38_001540"
     CDS             complement(7754..8590)
                     /locus_tag="IWO38_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0313633.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8607..9494)
                     /locus_tag="IWO38_001541"
     CDS             complement(8607..9494)
                     /locus_tag="IWO38_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0313634.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9510..10793)
                     /locus_tag="IWO38_001542"
     CDS             complement(9510..10793)
                     /locus_tag="IWO38_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0313635.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11476..12159)
                     /locus_tag="IWO38_001543"
     CDS             complement(11476..12159)
                     /locus_tag="IWO38_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ0313636.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12177..12794)
                     /locus_tag="IWO38_001544"
     CDS             complement(12177..12794)
                     /locus_tag="IWO38_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ0313637.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12804..13628)
                     /locus_tag="IWO38_001545"
     CDS             complement(12804..13628)
                     /locus_tag="IWO38_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ0313638.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13621..14181)
                     /locus_tag="IWO38_001546"
     CDS             complement(13621..14181)
                     /locus_tag="IWO38_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313639.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14354..15310
                     /locus_tag="IWO38_001547"
     CDS             14354..15310
                     /locus_tag="IWO38_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0313640.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15615..16571)
                     /locus_tag="IWO38_001548"
     CDS             complement(15615..16571)
                     /locus_tag="IWO38_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ0313641.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16675..17277
                     /locus_tag="IWO38_001549"
     CDS             16675..17277
                     /locus_tag="IWO38_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0313642.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17365..18864)
                     /locus_tag="IWO38_001550"
     CDS             complement(17365..18864)
                     /locus_tag="IWO38_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ0313643.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18880..19404)
                     /locus_tag="IWO38_001551"
     CDS             complement(18880..19404)
                     /locus_tag="IWO38_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313644.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19702..20451
                     /locus_tag="IWO38_001552"
     CDS             19702..20451
                     /locus_tag="IWO38_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ0313645.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20442..21227
                     /locus_tag="IWO38_001553"
     CDS             20442..21227
                     /locus_tag="IWO38_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0313646.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21196..21912
                     /locus_tag="IWO38_001554"
     CDS             21196..21912
                     /locus_tag="IWO38_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0313647.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22014..23033)
                     /locus_tag="IWO38_001555"
     CDS             complement(22014..23033)
                     /locus_tag="IWO38_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ0313648.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23052..24110)
                     /locus_tag="IWO38_001556"
     CDS             complement(23052..24110)
                     /locus_tag="IWO38_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ0313649.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPLDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(24331..25479)
                     /gene="nagA"
                     /locus_tag="IWO38_001557"
     CDS             complement(24331..25479)
                     /gene="nagA"
                     /locus_tag="IWO38_001557"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ0313650.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(25555..26391)
                     /locus_tag="IWO38_001558"
     CDS             complement(25555..26391)
                     /locus_tag="IWO38_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0313651.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(26575..27792)
                     /locus_tag="IWO38_001559"
     CDS             complement(26575..27792)
                     /locus_tag="IWO38_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0313652.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            28175..28258
                     /locus_tag="IWO38_001560"
     tRNA            28175..28258
                     /locus_tag="IWO38_001560"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28209..28211,aa:Leu,seq:gag)
     gene            complement(28498..29496)
                     /locus_tag="IWO38_001561"
     CDS             complement(28498..29496)
                     /locus_tag="IWO38_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313653.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         8842 a         6304 c         5065 g         9359 t
ORIGIN      
        1 tatttttgct attctatttt aattgaattt tgcaactcac tcttctttat tccaaaccta
       61 ctcgtttgaa tatcacgtca acgtttttca aatgatgatg ataatcaaag gcatcgtcta
      121 tttcttcttt agataatacc gatgtgatgg ttggatcttc ttctaacagc gggcggaact
      181 gcacttggtt gtcccaagcg tacgcagttt ttggctgaac aagatcataa gcttcttctc
      241 ttgtcattcc cttatcgatc aatttcagca taacgcgctg gctataaatc aaaccaaatg
      301 ttgcagacat attccgcttc atgttttcag gaaagaccgt tagattttta acgatattgc
      361 caaaacgatt taacatgtaa ttcaataaaa tcgtggtatc aggcaagatg attcgttctg
      421 ctgaagaatg agaaatatct ctttcatgcc ataatgtgac attttcatag gcagtgacca
      481 tgtgtccgcg caccactctg gctagaccag ccatattttc tgaaccgatc ggattccttt
      541 tgtgcggcat agcagaagaa cctttttgac ctttcgcaaa aaattcttct acttcacgtg
      601 tttcggattt ttgcaatccg cgtatctctg ttgcaaaacg ttcaatactt gtagcaataa
      661 gcgccatcgc tgagaaatat tcagcatgta gatcccgagg gagaacttga gtagagattt
      721 cttgtggacg gatccccaat tgtttacata catactcttc aataaatggc gggatatttg
      781 cgaacgttcc cactgctcct gagatttttc ctgcttctac tccttttgcc gcatgttcaa
      841 atcgttcgat gttccgtttc atttctgaat accatgtggc aagtttcaat ccaaaagtag
      901 ttggttcagc atggacaccg tgtgtccgac ccatcatcac tgtatatttg tgttcttttg
      961 ctttttcgcc aatgatttcc gtaaaacgtt tcaagtcttc ccgtaaaata tcatttgctt
     1021 gcttgatgag atagccataa gcagtatcta cgacatccgt acttgttaga ccataatgga
     1081 cccattttct ttcttctccc aatgtttcag aaaccgctcg tgtaaacgca acgacatcat
     1141 ggcgtgttaa tttttcgatt tccaagatcc gatcgatatc aaatgatgca tgctcacgaa
     1201 tttttttcac atcttcttgc gggatttcac caagttctgc ccaagcttca tctgctaaaa
     1261 tctcgacttc gagccacgca cggtatttgt tctcttccgt ccagatattc cccatttcag
     1321 ggcgtgtata acgttctaac atgttttttc tcctctaatc ccaaatattc gtttgtttga
     1381 tttcttccag cgtttgctgg atgtcttctg tcaagatagt gatatgtccc atcttgcgac
     1441 ctttttttgc ttgttttttt ccatagaaat ggaattgcca gtctggctta ttctcaatca
     1501 agcgatagct ttccagcagt tgttctccca aaacattgac cattactgca ttgcttatca
     1561 aacgtacttt ttctcctaat ggccaaccac aaatacctcg aatatgggca tcgaactgac
     1621 tcatagaaca agcttcaata gaataatgtc cagaattgtg aggacgagga gctaattcat
     1681 tcacatatag cccgcctgtt tttgttaaaa acatttctac ccctaaaaca cctgaaaggc
     1741 tcactgcttc tgcaatcaca cgggcaatac gctcagcctc ctcgatcaca tcctgatcaa
     1801 tcgcggctgg agcaatcgtt tcatgaagaa tattgttgtg gtggatgttt tccacgaccg
     1861 gaaaagtcgt gaaatctcct tgaccatttc ctgccaccat gatcgaaatc tctttttcaa
     1921 acgggatcca tgcttctaaa acacaggttc cttctcttaa aaggtccatg gcaggagcta
     1981 gatccgcccg acttttcaaa acatactgcc ccttgccatc atagccgcct cgagtggttt
     2041 tcaaaacaca aggatagccg atcccgtcaa tcgcatcttg gatatccgtc ggactgacga
     2101 tcgttgcata aggagcaatc acgatgttat tcgtttccaa aaaagatttt tctaacaggc
     2161 ggtcttgcgt aatcgcaagc aagtctgtcc cttgaggaat aaatgacatg ggtaaaatcg
     2221 catttaacgc ctctacactg acgttttcga attcataggt aatgacatct gttcttctgg
     2281 caagctcttt cagcgcaaag gtgtcatcat agacactttc aatgtgccaa tctgcaactt
     2341 gtgcggctgg acaatcgctc acaggatcca gcacacctac tttaaagccc atttcttttg
     2401 cactaatcgc catcatacga cctaattgac cgccaccgac gatcccgatc gttgaaccag
     2461 gcaacaaagg tttaaccaag ttgatcacta ctttctataa cagttttagc caacattgaa
     2521 cgtctttcag ccaatttagc ctccaattct aaatcataca tggaaagcat ctgtatcgca
     2581 agtagtccag cattaattgc tcccgctttt ccgattgctg tagtagctac aggtactcct
     2641 ccaggcattt ggacaatcga aagaagcgaa tccaaaccat ttaatgttcg tgattggacc
     2701 ggtacaccga taaccggcaa agttgttttt gatgcgacca tacccggcag atgcgcggct
     2761 ccccctgctc ctgcaataat gattttgatt cctttttttc gagcgttttg tgcatattca
     2821 aacatcaaat ctggggttcg atgagcagaa acgacctttt tttcataaga cacaccgaat
     2881 tcatccaata tttgacatgc ttctttcatt gtttcccaat ctgaagtact ccccataatc
     2941 acagagattt ttgctgtcat tgtttttcct cccagttttt ctacattcat attttaacaa
     3001 tcactttttt tgatgtcaaa gaaatttcga atgttaacaa agaaaaacat tttattgttc
     3061 gtgtttatta aagataaaaa aaaggaatgt gacaaaagtt aggtcacatt cctttttatg
     3121 gataaacggt gttctcctgc caactcctcg gtcacaagga acaatattca acaaacatga
     3181 gaagcttttt tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc
     3241 ttccttcaac ggtgttcaat cagctgaccc tcggtattag ctcaaacaaa cggcaaaata
     3301 cgaggaacta tttttccgtt tgtttctcta acaatcaggt caaaacgctg atttcacaac
     3361 ctttcttttg ctctattact tcttgaaggc ttcttccaat atttcttcat cttttttcaa
     3421 tccattaaag atcaaattca atatgatagc cgtaatgcta ctcattacga tgccatttcc
     3481 agtgaacatt tgcaatgttt gcggcatttg actgaataat gtcggcgtaa cattgaaacc
     3541 cagcccaaat ccaacagaaa cagcaacaat caatagattt ttatcattgc tgaaatcaac
     3601 ttccaacagc atacggattc cttggactgc caccatcccg aacatgacta acattcctcc
     3661 accaagtacc ggctcaggga tgatttgtgc taacgcaccg attttaggga ataatcctaa
     3721 aacaatcaag aagaatgcag agaaatagat gggttttctt gttttgattc ccgaaagttg
     3781 aaccaatccg acattttgag aaaagccagt ataagggaat gtattgaaaa taccgcctaa
     3841 gataacggca agtccttctg cacgatatcc ctttttcaat tcttcttctc caacattttt
     3901 cccagtaatg tcaccaagtg caaaatatac acctgttgat tctaccatgc tgaccatgga
     3961 aatgatgatc attagtaaaa tcgaccatac gtcgaaagtt ggtttaccaa aataaaaggg
     4021 ttgtggaaca tggaacaacg gcgcatctcc aatcgcgctc agatcaatca ttcccatgaa
     4081 agcagctaag atgcttcctc cgactaaacc aatcaatacc gcgattgaac gaatgaaccc
     4141 ttttgccagc atttgcacgc ctacaattag gagaatcgtc acaaatgcaa gtagcagatt
     4201 tgttttgtca ccaaaatcag ctgctgttgc attacctcca cccattttct cgattgctac
     4261 aggaatcaaa gtcaaaccaa taacggtgat aaccgttcct gttactatag gagggaatag
     4321 ttttttaatt ttagaaaaga caccagaaac taacacaaca aagatcccag aagcgataat
     4381 agatccgtag atagctccta ctccttcatt ggttccgatc aagatcagtg gagctactgc
     4441 ttggatcgca catcctaaaa cgacaggcaa gccaattccg aaaaatcggt taactgtcaa
     4501 ttgcaataat gtcgctaatc cacacataaa gatatcaatt gaaatcaaat aagtcatctg
     4561 ttcttgatta aagcctagtc ctgtaccgat caaaagaggc accgctactg ctcctgcata
     4621 catcgctagc agatgctgca gccctaaaac cgcagctttt ccattttgtg tttctttttc
     4681 cacgattatg cgtcctcctc aagaaactca acttgtccat tcgtcaagga agcaatacgc
     4741 gcaagagaaa cgactctcaa tcccatttct tcaagtaact gacgtccatc ttggaaagac
     4801 ttttcgatga cgatcccgat tccttctact tgcgctccag cttgctggca taattcgatc
     4861 aatccttttg ccgcttgtcc gtttgccaag aaatcgtcga taatcaatac tttgtcttct
     4921 gaagataaaa atttacgaga aatagaaatc gtacttgtca cttgtttcgt aaaagaataa
     4981 acagaagctg tcaataattc ttcatccatc gtcaaacttt ttgcttttct agcgaagatc
     5041 attggaacat ctaattctct tgcagcaaat aaagctggtg cgatcccaga agcttcaatg
     5101 gtcacaactt tcgtgattcc ttgttcttta aatacttctg caaaccgttt tcccatttgt
     5161 tccatcaata cagggtctac ttggtgagtg acgaagctgt cgacttttaa tacgccctcg
     5221 cctaatatac gtccgtcttt ttgaatacgt tcaactaatt ctttcacttt atttgctcct
     5281 ttatcattta ttaataccga aataaccgct tttgtgcaaa aacctactat gtttctgtta
     5341 aagaaaaaca ccctccactt tatcataaga aaagaagggt gaaatagcca atcttttact
     5401 tatagtccag catttacggt actggctaga aactgtcatc catattatga cgatatataa
     5461 gtaggtatgt aaaactaata atagtgtaaa ttcattttct tgcaactgtt ttgcacgtga
     5521 aggcagtttg ccatatttga ataaaaaatg ttgtttcaaa tattgaaaac agtctaacac
     5581 tactttctga aactgacaac gtggtttact tatttttttg taaaatctaa tgatttttaa
     5641 tgcattattc ctttttgttc gggtttaaat agttattttt attttctcct atctaaaaaa
     5701 gcagcccaca taaaaagaag gcatgacaaa aagcatgtca tagccccttt ttccgaataa
     5761 atagtgttca aaagacagcc tatcggcaat aaggcaagat tttataaact acgggaagta
     5821 atttccgtaa atctattctt attcttttaa gctgacaagc tcgtcaatca ctactgcttt
     5881 tcctgaagca atagaatgat ttgctgcgat tcctgtcaaa atcgacaaaa taccatctac
     5941 gtgagttgct gcccgattcc acggatcttc ttctttttct tcaccaaata gatcatctaa
     6001 taaacgcgga tctcctccgc catgtccgcc cgcttcttgt tcgacgatta cctcatatgg
     6061 ctcaccgaac atcggataga catggatgga ctgaagaacg gctgctcctt catccttttt
     6121 atctccacct gcattgatgt aagacttttc gatcactttc atttcgatcc gtcctttgct
     6181 gccattgaaa gaaacattga atccttccca cggcatatac gcgtttagcg aatacgtcaa
     6241 aatcgcacca gtttcatatt tgaccgttac gcctagtgta tcttcaatag agatatcatc
     6301 ggaaaataca ctgcggtcac gaatatatcc actgtctttt tctgcttcta aatacatttc
     6361 tcgcaattcc tcattggctg acaggtctat agcaaatgga tcagctttag ctagttcgct
     6421 tccatagcat cgactgtaaa atatttctac tccgcgtttc tccgcattat gcatcccata
     6481 aaagtttaat ccaccgaatg caaatacctt ttggggacgg ctgttcaacc aaaaattcac
     6541 tagatcaaaa tgatgggtag atttatgaac aagaagcccg ccgctattgt gcttatttcg
     6601 atgccatctt ctgaaataat ctgccccgtg ttgcgtattt aacagccatt cgaagtggat
     6661 agaaaacaca tcaccaatca catcactttc tatcagttcc tttatcttcg tgtgatacgg
     6721 tgcataacga taattaaaag tgactcgcag gcttcttccc gtctgttttt ggaccgttat
     6781 tatttcttga gctttttcct catcgatagt cagaggtttt tcgcaaataa catcatatcc
     6841 ttttttcatt gcacgaatga tataatcatg atgcgttcga tctactgacg taataatgac
     6901 ttcagccggc cgtacgtcat ccagtagttg ctcaaattgt gatggcatac atgtctttac
     6961 ttttttctgt ccatacttgt tcacaagtat ccgatttgca taatccatcc ttgtttgaga
     7021 tacatcacaa aaaccgacta gttcacaagt ttgatgatac gtagaggcaa ttgcctcata
     7081 aaaaaaacgc gctctcccac caagtccgac ttgtacatat tttttcttca tttaaagtca
     7141 ttctcctttt cttttaagca aaatactgtg acaaatggca gccctggata cagtaatgca
     7201 gtgagtaaaa aatcatgctg tatacaccag aacagtccat acaaaaggca taaataccac
     7261 agaaacccct tgacgttttt tgacaacagc cagatattcc ttttgatcaa ataaacaaga
     7321 ctatctgctt tatcttcttt gcagatatca attagcgtac taaaaaaact atgggataca
     7381 aaaagcaaca gacacaatag aaaaggaatc agataccaag ctccaatctt tcccactaat
     7441 aaataccaat caactgcaaa aataatgaca aacagctgaa acaacgtaat gatgaaacac
     7501 atccgtttga tttttccttt cctccaacat accttttttt ctgccaagta atggacagat
     7561 agataataag aaggatacat catcaaaaaa taaatcagat agacaacacg agcagacagc
     7621 actaatccta aagtgaacaa acatcctaga actaaagcga aaagagcatt actgataaga
     7681 ctggttaaaa aaatccgaca gacatagtag ttgagcttgt aaagaaacat ctcttctatt
     7741 ggctttttca tattcagccc ttcagaccgc ttgtactgat cccctggaca agatatttat
     7801 tgaagaataa gaaaatcaga aaaaccggta gcagcgacaa cgtagacatc gcaaacatac
     7861 tcgcgtaagt cgtactagat ccagcgtctg caaataattt gatggcaatc gataccgtgt
     7921 aagatctcgg cttgtttaaa taaatcaatg gtcccatgaa gtcgtcccat ttccaataaa
     7981 actggatgat gatcgtcgtg atgatcgaag gtttcagcaa aggaaaaata attttggtaa
     8041 agacactata cttgctgcat ccgtcaataa tcgctgattc atctaactct ttggggatgc
     8101 tgaccatgaa ttgcatgatc tggtaaatga aaaatgcttg tccaaaaaaa tgtggtaaaa
     8161 tcagcggcac atacgtccca accaaattca gccgattgta aataataaac tgcggaatca
     8221 aaatcacttg tgcaggaatc atcatcgtta ctaacatgac gacgaaccag aatctttttc
     8281 ctttgaaatc gattctcgat aatgcgtaag caatacaagt cgagctcaga actgtcccta
     8341 tcgtcgaaac tgcagagatg acaaacgaat tcctaaagaa agtaaaaaat gaaattccag
     8401 caaaccccgc ccatcctttt gcgtaatttt cccattgaaa agtctctgga atcaaattca
     8461 gcgaaccact cataatctct tgatccgttt taaaagaacc tgcgatcatc cataagaccg
     8521 gataaatcat gactagacct aatagcaaaa taaaaccgta ttgaaaccat ttcgttactg
     8581 tattattcat ttcttcctcc tctcattcac acaacgaccc atttttttga tgatttgaaa
     8641 atcagaaagg tgacagctcc cataatcaac agcatgatcc acgaaatagc actagcatac
     8701 cccatagaat taaaagaaaa agcttggtta tacacataaa gagcataaaa gttcgtggca
     8761 tcattcggac caccttgggt aatgataaat gcctgcgtaa aagtcataaa cgccgcgatt
     8821 gtctgcataa ccaagttata caaaatgatc ggcgaaagaa gaggtaacgt gatcttgtaa
     8881 aacgtctgcc atttgttcgc tccatcgatc tctgccgctt cataatacga gcctgggatc
     8941 tctttgatac ctgcagcaaa gatgatcatt gccgaaccga attgccaagc agacatcaaa
     9001 acaagaggga tcatgaccag tttttgatcg ctaaaccagg aaagcgcagg tgcgcctaat
     9061 cgttccagga aaatattcac taacccgttt ttagaaaaca actcccgcca aactagagcg
     9121 atagaaatac tgccaccgat caaactaggc acataataca aagaacgaaa gaaagtcgtt
     9181 cctttgatat tcctcgttaa aagataagca atcaatagac caaagatcag tttcacaggt
     9241 acagatgcaa atacatagag aaaagtgact cggatggagt tcaaaaaacg ggcatcactc
     9301 agcatcttta tataattatc caagccgatc catgagactg gatttcccaa acgataatcg
     9361 gtgaacgaat aataaagtga caagatcatc ggtatcactg ttatcaatag aaatcctagg
     9421 atgaatggag aagcaaatac gtaaccgacc acattgtctt gatccaagaa ccgttttagt
     9481 ttttcttttt tcatgccttc ctcctgctgc tatcctagaa tgtttgacgc aaactcataa
     9541 agactctctg ctgcttcttc tgctgttact tcttcataaa tgactttttc aactaaaaga
     9601 ttgtattggt cttgaatttc cggattcaaa gcactatcaa agacattcac ttcgccgttg
     9661 tccatatcgg aaatcatttc taaataatca aatgtcgctt gcaaagttgc atcatctgca
     9721 cgagcgagct gttctcgaat attcgacatg attggtacac cacgttctcc ttttaaaatt
     9781 tcattggctt cttcactgtt ttgaaagaaa tcaataaact tagcagcttc ttctttatgg
     9841 gctgagcttt ttgccatact cagcatttgc gaagaacgta aagggatacc agattctcca
     9901 tctgcttttc tgcgaggtgg attaatcaac tgaatctcca tatcttcagg agcccctgac
     9961 aatatttctg tcaactggtt acttgataga aacaccattg ccgcttctct atccctaacc
    10021 gcctgaaaag acagatccgt cgtacttgca gctccaccac ctggttctgg atgggcccct
    10081 ttgtctacca gatttttacg cagctggaaa aaatctgtta gatagtctgg attttcaaat
    10141 cccaagctgt tagatagatc ttcccgatta taaaaattca atcctttctc aacttgtggt
    10201 atttgaataa tacagccagc tataaaatct tcgaatcttg aaatagcata gattcctaat
    10261 tcttcagtga tttgatagct gatttgttca aattcatccc atgtccaatt tgaagctggt
    10321 tcagggatat ttaattgttc aaatatattt ttattgtaag caatcgcaaa cgaattcacc
    10381 ccgttagaca gtccaactaa ttgcccgtcg taagtcgtcg ttgctaaata ggattcatct
    10441 gcattagaaa tatcaattat cccttgctcc acatattcat ctaatggttc aatctgattg
    10501 atgtattgag gaaattgatt gttcagttgg aaaatgtccc agatatcatc tgatgctgct
    10561 agtgtaccta attttgtgta atatccttca taatcataaa attcataatc gatcgtcaca
    10621 tggggatttt tttcttcata aagctcgatt acttttactg ttgcatcgtg tcggacttgc
    10681 gaaccccacc aagccatgtt cagcgtgatt tttttgtcat cttcttgccc acaagccgtc
    10741 aagaagaata acataacact taaagctaag atgattctca ttcttttctt cattctctgc
    10801 acctctactt ccattgttat ttttcaatat actgtatttt tatacagaac atatttatta
    10861 aacgatcttt ttcaagggga ataaaaacca agacttgcct tatcgttctg cctaggatga
    10921 acaactcaat actttcaaga aaaattagat agtgctgatt cttctttcat cgctaaaaac
    10981 acttcttgct tcaatgatgg gaggataatt acgcaaagaa aaaaggatat cagacaactg
    11041 cccaatatcc ttgacatagc taaataagca tgcttcttcg tgtgcgctct tccctaaaaa
    11101 acagaaaaat aactagtaat tctgtatgaa attaggtaca atatacacgt cgatgcacaa
    11161 gcattgacta tggcaacttg attggtcagt tcccgctgac ttttcaagcc ccgacaattt
    11221 agctgctact aaattgtcgg gtgccttttt tagtttttat tcgtttttca ttctatagaa
    11281 agcgcttcaa taaatcaaga catttttttg ttagaatgct aataataaca aaaggtttta
    11341 actcttctta tgtacattac aggtaaaaaa acaaaaaaat ttctcttttt tttgaattct
    11401 gaacaaatag tactggagta caaaagacga ggaagagcca ctatctgctc ttcctcgtct
    11461 tttgattctc acctgttatt catgattctt tttctcagtt tcccgatatt tttccatttt
    11521 acgactaaaa agctctccgc tacttggtgg cgtatatgtg atggcatttg ctcctgcttg
    11581 aatggtctca gcaatacttt cttccgttgg accacctgtc gcaatgatag gaagttcagg
    11641 atatttttta cgaaaatggc gtactgcttc tgcagtttct ttacctgcac tgatattgat
    11701 aatcgaaata ccagctgcta acttttcatc tatttctgta tatttcgacg ttaccgtact
    11761 gataactgga atatcaacta ctcgacaaac ttcttttact gtctctaaag gcgtaggacc
    11821 gttcaaaacg acccctaacg aaccttgcgc ttctgcgaac atgctcatgt aggaagaacg
    11881 agcaccttgt gtcaaaccac cacctacacc ggagaaaact ggaatgtcag ctgcctcaat
    11941 aatgctttta gtaatcgctg gatgaggtgt aaaaggatac acagcaatta ctgcattcgc
    12001 atttgtatta cgtatgatcg caatatccgt agtaaagatg atcgaacgga tcaattttcc
    12061 aaatatcttg atcccgcttg cttctaaaat gatttccggt actcttacga tatcttttct
    12121 caattccgtc atgatttctg gcgtaaattg ttctgccatc agccacttct cctttgctat
    12181 ctccggtcat actgattcaa ttgatacgtc tcgatgacct ggatgatttt ttgcgcataa
    12241 ccggggtctg tcgcataacc tgcatcttgc aatgcttgtg cggcttcctg atagttggtg
    12301 gccgtaatca ctttttcata cagcgcagga ttccaatcta ctccttggac gaacagttgt
    12361 gtatggtcat ccatggactg ttcccatgaa tcatacactt tgaagtctcc ttggatagtt
    12421 atccattctt cgttaacaaa ttcctttgtt tccaggctta ctttcttctg atccccataa
    12481 gccttgatcc cgaaaagatt attgtatttg ctagccaagg tactttttcc ccaatttgat
    12541 tctaatatag cttgtcctaa aatgatacta ggaagcactc cataaccatc ttgtagttct
    12601 tgggcatgcg gaacaagccg ttcaataaat tgctgatggg attgcgcttc attttcttgc
    12661 tgattatcag taaaaggaga agacagattc ttcaaggaaa aaacaaaggc taagagtatc
    12721 actgctacac cggcaaccac tgctggccaa cggatattct tgcgcttttg ctttttccac
    12781 ttttttttcg ccatgatcaa ttcctacttt tcatttaatt cattcagata ttcttctagc
    12841 gtcaattggt gtttggtgat atactcagcg ttttctttac cgacataacg taagtgccaa
    12901 ggttcatatg tgatcttagt tatatcttcg tgtccttttg gataacgaac gataaatcca
    12961 tactgcgcag cattatctgc gatccattta gctccaggct gatctccgta acttgcatcc
    13021 aatacagtag aaggataact gttgtaccag ttttgatcga cgacatcgac tgccagacct
    13081 gtgtggtgtt cgctgtatcc tggctccgta atcgtttctt tagtaatctt tgtcgcctcc
    13141 tcttctgata caccttgacg actcatcaca tcttggacat tttgttcgaa aacttgttgt
    13201 tgggatgata cagaccggta agctgagacc atcactaatg gaaatccagc tgcttcagca
    13261 gccttggcaa atgcttcata ttctggagca atgcgtttgt cgatcaagta tccgttactt
    13321 aatgatgcca attgagtaga ctcatcaatt tctttttcta atttgtgatc tggaccaacc
    13381 aaaactagct tccaatcgtc tgttttaaca tctggcaatt gtttggacgt acgatctttt
    13441 gtttctttgt cctttacctt gctgctggtt gtttgactgt tcttttcttt actagcagtt
    13501 gttttggtgc ttgttcgttc gactgcttga ttgccggctt gaacatgcga atcatcatcg
    13561 ctaaaaataa catagcccat tgctgcgatc ataatcacaa ctgctccaaa tcctaaaaat
    13621 ttattcactt gaaaaatcct ttcgttgttc ttcacgttcc ccattcgcct catgaacgta
    13681 agtttcttcc agattttcat tcacccaatc gagaagttcg attttttctc cttcgatctg
    13741 tttttgaaca tctgctggca gacgaaaatt caaaatatca tcatagcccc attcatcata
    13801 ttgaatactt actttcaagt caaggtatct accagcctgc tgattatttt tcaaagggat
    13861 cagctcgact tttgctgctt tgctgctaag ccatttttgc gcgatgcgtg ttttgatttg
    13921 cttgtactct gcaacggcaa cgtttcgttt ttcgggaaaa ataatcgttt gacgcaaaat
    13981 cttttgcgtt agcatccatt cataatcagt gaaaagattt tttactttct gcatcgcttc
    14041 agatggcaga tttttctcgc catctttcca tttttgccat tgttctggac tgatcagcaa
    14101 gtattcatct aaaaaatttt tttcagtttc aaactgttct aataatacac tcataataag
    14161 atcaatatat acttgatgca cgtttatccc ctccttttaa ctattctact taagttttga
    14221 tcaaaaagca cctcttaaat cgctgctttc atttttttaa tgtttcaagt cctctcctct
    14281 ttattcttcg tgatttttgt tataataata gacagacaag aaactcaatc caactatgaa
    14341 ggagcgtttt cagatgaaaa aatatcaatg gggaatcgtt ggtcttggta ccatcgcaca
    14401 tgagtttgcc gaaagcttca atcaagagac gagcgaacta acagcagttg cttccagaac
    14461 ttcagaaaaa gcggaaaact tcgctcaccg ttacaatatt ccaaaagctt acggaagcta
    14521 tcaagaaatg ttagacgatg ctgaaatcga catcgtgtac attgctgtac caaatagaca
    14581 gcatatcgac catatcttgg cagcattgga agcaggcaaa cacgttctat gtgaaaaagc
    14641 catcacgatg aataaaaaag aactggctga cgctatgcgc ttagcagaag aaaagaattt
    14701 gatcttagca gaagcgatga caatcttcaa catgccactt tatcagcagt tacgctcaat
    14761 aatggataca ggaaaactgg gggctttaaa aatgatccaa gcacctttcg gcagttacaa
    14821 agaaccagac cctaaaaacc ggttcttcaa tccagaatta gctggaggag ctctgttgga
    14881 tatcggtacg tatgctgtat ctttcgctcg tttctttttg agctctcaac ctgaagtagt
    14941 tgcctctaca atggttcctt ttgaaacagg cgtggacgaa caatccgtca ctatcttgcg
    15001 taacaaagaa aatgaacttg ctgctgtcag cttgactttt caggcaaaaa tgccaaaagt
    15061 cggtatcgtt gcctttgaga acggttatgt gacgattgct gattacccgc gtgctgatcg
    15121 tgcggagatc ttatttacag atggtacaaa ggaattcatc gaaagcggga atacatctga
    15181 agcaatgaat tacgaaataa agaatatggt acacatgatt gaaggtgact tgcccaatcg
    15241 ctctctcttc ttgacaaaag atgtcatcga aatcttggat caaatgcaag ttctatggaa
    15301 aaatatgtaa ttttaaaagg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagcc
    15361 aaacaaaaat agctcctcct atttttcgtt tggctggact tattaccgca gaggaaagct
    15421 tttgaacacc gtttaagaaa gaggttgtga gcctaccgtt cagctccgag tcacaaggag
    15481 caatttcaaa aaacagcttc tcatgtttgt tgaatattgt gacttgtgac cgaggagttg
    15541 acaggagaac accgtttaaa gaagaggttg tgaaatcagc tttttgacct gaatattagc
    15601 tgaattcaca acctctattt ttggattttt tgttgggaga attctgcctt gatttcaata
    15661 aaacttttaa aagactgcaa aaactggaac aattctgctc gatcctcgaa ttcagggcga
    15721 ctagtcggca gaggcatttg ccgatacaat acataaactt cttcaatacg tgttaaaatt
    15781 tgtctgccat catttgactc tgaaaatgtt tctgcagtat aaatcaataa accatagata
    15841 tgttttccat aataagctgg ttgctggatt ctctctagat tttccagcat gtctttaatc
    15901 acattggcct gtgctcttct catagagaag taagtttcgt agtatagcgg ctgtctgagc
    15961 cagtagtttt cttgatgttc tctggcatct tcttggcttt cgcgaatgaa tgtcagtaaa
    16021 tgttcgcatt tgatctgcac atcttcttta ttttctgata aaagaaattc tgccatttgt
    16081 ctcaaaatat ttcgaaattc tttttcgatc tgtatttggt tttccttcag ctgcttcgta
    16141 cgatctggca tgtaaacatt tgcaagcaaa gcaaatacta atccaatcgc cattaatccc
    16201 gcttcattgc cgatcagctg ccaagacatg ttttcctcca ctaaataatg agtgaccaaa
    16261 accgaattta ctactatccc ttcggttaat ttgaagcgag aggcaagcgg gatgaacagg
    16321 aataaaaaaa gactgaagcc aaaaatagta tacccaacag caaaaaaact gaacagtgct
    16381 gttgccgtag ctaatataaa ggctgcgatt cgattcatgc ccgtacgcaa tgtagagcgc
    16441 ttggtatttc ctacacttaa taatgcaatg atccctgcag ctggccaata caacagcgat
    16501 aagctacccg ctgtgaccat cgaaaagaag acactgatca ctgttttgac tgttcgcatt
    16561 cctatacgca tttacttccc ccttttttat tttaattata cttgaataat gaaaaactag
    16621 ctatcagaca aaagtttagg tatgatagta aaaagaccaa aaaggagaat ctctatgcaa
    16681 tttggccgtt ttcgactagg tatgcgtacg atcaaaagtg ccttagctgt ttttttatgt
    16741 atcttgtttt ttcatttgac tcaacgagga atcccgatga ttgctgcatt atctgctgtt
    16801 ttttctttgc gtcaggactt gactacaacc gtttcttttg gaaagtctag gatcatcggc
    16861 aatacattag gtggtacatt agctatcatt tatttttttg tcaaaagcta ttttcacaat
    16921 gattttttgg tagaattgat cttgcttcct attctagtta ttattgtgat cgtcgtgtca
    16981 gatggtatta acaataattc cggtatcatt tccgcaatag ctacgttgtt gctgattgcg
    17041 ttgagcatcc ctcaaggcga atccgctttg tatgctatcc aacgtgtact agatactttt
    17101 atagggacat tcattgcaat cggtatcaat ttctttcttc gtcctcctga aacagaaaag
    17161 aaggaagaac tatcagaaga tttggtcgaa ctccaaaaga aagaacgatt gctaagaaca
    17221 aatttacaag agatagaagc taagatccaa aaacaaaaga aaaatagcga ggactaattt
    17281 tcttttgcaa taaaataaaa aaatggagag aagaacttta aaaagtcttc ctccattttt
    17341 gattgaacgg tgaagtgtac tactttattc ttccttcatc gagttttgtt ctgcctcttt
    17401 tggcgcttca tacatattta cttggctagt agtcccacgt gtcgacaaat tctctacgat
    17461 ttccttcaag tcaagtcctg ttgtcgcttt tagtgtttct tgagtagaag aaagcaaatt
    17521 tgtggcataa tttgtcactc ggttcgctcc actggtttct cctgaactgc ttcctgtatc
    17581 gacaacagag attttttcga tatttcctag cggctgtgct gcttcgcgca tcaattgcgg
    17641 tagcatatcg ataaccatac tcaagacagc tgcttcgcca tattccttga aggcattggc
    17701 gatcttttgt tttgcttcgg cttctgcttt tccttttgct agtgctgctt cggcttctgc
    17761 ttgtccagct aaacgtgtct tattggcttc cgcttctgcc agtgcttcca cacggaatcg
    17821 ttctgcttcg gcttcagtca cttctttgac tttttgagct tgtgcttctt gttctttagc
    17881 gtaccgatcg gcatctgctt ttttcttcac ttccgaatca tattgctttt cacgacgtgt
    17941 gatttctttt tcttccagtt cgatttgttt ttggcgttct acgattttaa tctgcatttc
    18001 ctgttcgatt acttgttgtt gtgctcgggc actttctaaa ttgtaggctt ggtctgcttt
    18061 tgctttagca acatcttgtt cttgtttgta ggttgctaat ttcagttctt tttcttttaa
    18121 tgattctgcg atctctgttt gtcgctgtaa ttcagctgct tgtgattctt tttctgcttg
    18181 agcttttttg atacgcgttt cttttagagc ttccgcttct gctatatctg catctctttt
    18241 gacttgggcg attcttggtt tccctaaaga gtcaagataa ccgtttttgt ctctgacctc
    18301 tttgatagtg aaagaaacga tgatcaaccc catttttgca aggtctacag aagcgacttc
    18361 ttgtacgctt tgactgaact tatcgcgatt ttgatagatt tcttctacag tcatagaacc
    18421 aagaatcgag cgcaagtgtc cttctaatac ttctcttgct tcattttcca gttcttctct
    18481 tgtcttgcct aaaaactgtt ctgctgctgt agcaatctct tctacagatg aaccgatttt
    18541 gatgatcgaa gtaccatcac acatcaccgg tacgccttgt tccgtataca cttcaggggt
    18601 ggagacgtcc aatttactcg ataatagact gatccgattc gaacgttgaa aaactggcaa
    18661 gacgaatgcg ccacctccac gcacaatttt gattttgtta ttgctttcat caatgtgcac
    18721 atttttgctt cctaaataac ttccactgat gatcaacgct tcatccggtt ttgctgtctg
    18781 gtacttcgct acaaagacga tcagcaacat cagtaaaatc aaagctgcga ttgcgatcgc
    18841 gataaaaaca actggtattt ccatttaaca cacgctcctt taagctttta tcatgctttc
    18901 ataaggcatg acataacaga ttctttcctt gatctcaatg atcaatactt tcgttccccg
    18961 ctcagctgtc tgttcttggg ggctgtaaaa acaagctgga cgactaatgg aaccggtaac
    19021 acttttcaac tgaatttctc ccatacctcg tacagggatc ggtgtgatca caacagctac
    19081 ttgtccttcc agagtttttt cagaagacga catcgttgat tcggcatgtt tcatcggcat
    19141 caatatatag aaattaagca aaaataccaa cactatcgat atgcctccac tagcaataaa
    19201 taaaaataaa gatgattgat ctgttaaacg ctcacctaga aaaccaaata agcttgtgaa
    19261 agttaaccaa gggataataa gcatcggatc aagcgggcca tcaaaatcga atacatctcc
    19321 aaaaataatc aataggactg ccaatcctgc acaaatcagc aatgtatacc aataaatcgt
    19381 ctcaagtgcg attcccccta tcatatacac gcctccttcc atttctacgt ctatcatacc
    19441 aatgtttgaa aattctgtat agcttctttt accgactcag tggtgttttt tataggaagg
    19501 ttgtgtttat cgttgacaat ttgttaaatt tttgttttca tgttaaaatt ttcacaataa
    19561 attctttcga ggtagcctat gtttcctttt tttaagaaaa aaccattaaa gctccagaac
    19621 acgatcatcc ttgtcgtttg tttctccaca ttcgtttctt tgattgtttc tgccatattg
    19681 atcaggaatt ttattctttc gatgaaccga tctagtgtgt cagataaaat ctctagcatt
    19741 gctaaaatcg ttgccaatga taaactcatc caaaaaaagc tgcagactgg tgacccgaca
    19801 catgcgattc aaggaataac aaatgaaatc atgattgcat cgaatttaga ttttatcgtt
    19861 gtcatggacc ctgaaggcat tcgatattct cacgcagacc caagttacat cggtcttcct
    19921 ttctcaagtc atgaagatgt gaaaaaagtg attggaggac aagaattttt ctctgaaaaa
    19981 gaaggaatgt tagggttcgg cttccgctat ttcgtcccta tttattatga tcaagagatt
    20041 attggtgcag tttgtgttgg cttaacaaac aacacgattg acaatcaagt caaagatgga
    20101 caaaaaatga ttgttttttc attgattatt ggacttagcg taggcatcat aggcgccgtt
    20161 tttcttgccc aaaaaatgaa aaaagtactg cttggacttg aaccacaaga aatagcagaa
    20221 cagttaagtg aaaaaacgat tatcgaaaat gaagtagatg aaggaatctt agccatctct
    20281 tctgataaaa ctatcctctt gatcaataca cctgcaaaaa acattttcta ccaaacagag
    20341 cagctattgg atttcgaagt aggaaaaaaa tcagtgataa tctatatgac gttttgttta
    20401 aagagacgat tgagcatcaa acgaccgttc ggactcaatc catgtattta aatgggatag
    20461 aaactatttc tagcgttaca cctcttttta gcaatgaccg ttttttagga gcagtcgcaa
    20521 ctttacgaaa tcgatcagaa gtaatgaaat taatggagga gcttgggggg acgaaacaat
    20581 atataaactc catgagagct caaacccatg aattcatgaa taaacttcat gtcatttctg
    20641 gtttgattga tttgaagaag tatgaagaag tcacccattt tatccagcag ctaaatcaaa
    20701 attataaaaa agagattggc caagtctcaa gtctgatcaa agtacctgca attgccggtt
    20761 ttttattagg caaaataaac gaagccaaag aacaatctgt tgaaatgctg ttagaaagcg
    20821 attcttttat ccctaaaatg gagatggatg actccatcca ttcacttttg caagtgttag
    20881 ggaacttact tgataatgca aaagaagccg tgctacaaaa acatgaaaga aacggacagg
    20941 tccggattca tttaaactat gacacagaag ctgaagtatt catcgtgacc gtctgcgatg
    21001 acggtgtcgg gatatcagaa gaaatctcag aaaaaatatt tacgctaggt ttttcgacga
    21061 agggaatcaa tcgtgggttt ggattgaatc tcatccattc aatcgtcaaa cgccacggtg
    21121 gatttttaga attttccaaa caaacaaatg gcggtacaat cgtctatcta gaattaccaa
    21181 aaaaggaaga tcaaaatgat gacaaatgtc ctgataatcg aagatgatcc aatggttgca
    21241 gaattgaatc gtacttttgt ggaaagcatc cctaatatga acgtaatcga aagtttcaga
    21301 aatgtagaag aaggaaaaaa atatttcaga caaaaaaacg cagatctgct gctgcttgat
    21361 gtatacatgc ctggcgaaag tggtatccat tttttatctt ggcttagaca gcaaggaaat
    21421 gatatcccag tgattttgat cactgctgca gatgatatcg aaaccataaa gaatgtgatc
    21481 cgctacggtg ccttagatta tttgatcaaa ccgttcactt tgcaacgttt tcagctggcg
    21541 atcgaaaagt atcttgcttt ctctcagaca atccaaacga ctgaaaaagc cgatcagaaa
    21601 ttgatcgatc aagtattcaa ccacaccact cccattcacg aaataccatc attcatcgat
    21661 ttgcctaaag gtctgtcaaa actgacttta aaaaaaataa gtgcatttat tctaaaagaa
    21721 gatactattt tttcaacaaa agaactggct caaaaaagct cactgtcacg tatatccgtc
    21781 aagaaatatt tgaatttttt agtagaacaa gatatattga acgaaagaat caaatatttg
    21841 gaaatcggcc ggccgctaac ctgttatgaa ttgacaaaga atgggcggaa aaagctcaca
    21901 gattactgtt gagtcatcga attatcccat acaaaatagt ccaagtatac agaagtaaca
    21961 actgtttact tggactattt ttaaacattt tattgacgaa tatccatgtt ttcttatctt
    22021 aattgacggg ctttaggaag agatacctca tttttcgttt ttccagtttc tattatttca
    22081 cgaaagttct cgaaacttgt cgaaatcata tttgataatg cctcatctgt atttgaacct
    22141 acatgcgggg ttacaagaac tctagggtac atttctacta attgctggat agcagggtct
    22201 ggaatcgtct cccatggctt gaaggatcgg aaaaaaacct ctgtttcatt tgcaaaaaca
    22261 tcggttgcaa aaccagctaa atgattgctt ttcaaagctc ttaaaatagc ttcattatct
    22321 tgaagctctc cacgggctgt attaatcaag atgctacctt ttttcatttg agctaaaaat
    22381 ccttcattga tcatttgatc gttttcacct gggatatgag gtacatgaat actgacaata
    22441 tcgcttttgg ctaataactc ttcaagcgag tggaacgtca atactttttt agcagcttct
    22501 gtctgatgaa tatcgtagcc gatcactttg gctcctagtc ccttaaacaa aacagcttcc
    22561 gtcaacccta tcctgcctgt tcctatgatc cctactgtgc aatttctgat ttcacgacta
    22621 aacatttgct catccactcg aaaatctttg aaagatgttc ggatcgtcgt atacgcagta
    22681 tttctcaaaa gcatcatggc aaaagtcaaa gataactctg ctatcgcatt tggcgaatac
    22741 gatggtacac gggctactgt cataccaaaa tcagctgccg cttgtagatc gatatgattg
    22801 actcccacag tccgtgtaaa aacataacga atcccatatt cctgcatttt tgcaagattg
    22861 gtctcatctg ctatacaatt tcccctcaac aatacagcat catggtcctt tgctgacacg
    22921 atattgtcat gtgtcaacag ttcttcataa agactgagat cataatggta aatattcaat
    22981 ttttcaaaaa aatctatctc gttcggacga acaccgtaac atgcgattct catactttct
    23041 tcctccattt cttatgaaaa taaacctatg ccatgaatca tttgtaatac aacgatccat
    23101 acaggtgtca gtaccacagc aaaaatagtt gacagtaagg atgcattgga ggctaaaagt
    23161 gcctccctat caaaactgat agcataggct gctgccactg ttgcagctgg tgtagccatc
    23221 atgataacga ttgttgccag tgcttcaaaa ttgactggta gaatattcgt tgccgtcaaa
    23281 actgctagaa tcacgatatt caatgcaggt acaaatacca ctttgactag actgtaatac
    23341 catgaagttt tttcagatgc agcttttttg aagcttactt cacctaatgt tgcgccgatt
    23401 gctagccacg ccaatggtga acttaaacca gctaaataat ccagcggctg aaatagccac
    23461 ggtgcagtct ggtcaattcg caaaaacgct acttggctta tctctccttc tgcacctgta
    23521 actgacactt gtggcagata cccttggaac agccaaatga aaaggccggc aaaagtagcg
    23581 ataatgattg gattgacgaa catcgctttg atatttttag cttccatctt caagccactc
    23641 atttttatgt atccataaga atacaaaaag attcggtagc caatattgaa aattgaggaa
    23701 tacatcacgc cgacaggacc ataaatcgca ctgacgatag gcgttccgaa aaaggtcgta
    23761 gaaccaaaga tcgtcaggac acgcaacgtt gtttgcttat cccctttata gcttaagaaa
    23821 aaaggttttg aaactagaat aagaataata taaataacta atccccaaat caagacattg
    23881 attccttgca tcagtaattc tgtattgata tcttgcatga aagctcgaaa tgccagtgct
    23941 ggcaaagcca ctgttaagac gactttcgat agaatttttc caacagattc agaaaaaatc
    24001 cccttttttc tacaaacaaa ccctaataat ataataaaaa ctgttgaagt gattgcactg
    24061 acaatcgaca tatctgtcaa agtcgatcga aaaatgtcaa ataagttcat ctttctcctc
    24121 ctaattatgt tcatttattc acaataaaaa aagcttttat tttgctaata cgtattaaca
    24181 tttttaattt tatctttaca ttgttactta ttgaacaata ggaaattaaa gaaacttttg
    24241 aaacttatgt aaagtgattt ttactagaac acaaaaaaaa ccttaaaacc aaacttaaga
    24301 agtttagttt taaggttaac ttaaattata ttatgcgtgg aaacgttcca cgccatctag
    24361 ataagttgct tccaatgtca tatcagggtt caatacgata aagtctgcat cgcgtccttg
    24421 agcgattttt ccacatgtat catcgatttt acagctgatt gctggaatca gactagccat
    24481 catgactgct tgttctggtg ttgctaatcc ccagtctaca acatttttaa ttgcttcttt
    24541 caatttcaat atacttccag ctaagttgcc agattctaga cgtgctgttc cttcttttac
    24601 tactactgga aattcgccta aattgtaatt tccatcaggc atcccaccgg ccatcataca
    24661 gtctgtaatt aatgcgacat gatcatgtcc tgctttttcc attaggattc ccgcagcatt
    24721 tggatgtaca tgatggccat cacaaatcaa ttcagaaaag acatgttcta aagataataa
    24781 cgcacccacc atacctggtt cgcgatgatt caatccacgc ataccattga aagcatggac
    24841 aaatacactt gcgcctgctt caacagcttt ttgcgcttct tctagtgtag cattactgtg
    24901 tcctagagaa acaactactc cttcttcagt cacttgtttg acaaactctt ctacaccttg
    24961 acgttccggt gctaaagcaa tttttttgat taaaccacct gaagcttctt gccattcatg
    25021 gaaagtatcc aaatcaggat ccccgaaata acttgggttt tgtgcccctt tgtgttcttc
    25081 tgtgaagaaa ggaccttcaa aatagattcc tttgatttta gctcctggtg cttcctgata
    25141 cattttgccg attgtctcag ctacatcttt taaacgttct ttgcttgacg tcaatgttgt
    25201 tggtaaaaat gaagtcactc cacaagataa cagaccttca gacatcactt tgatgccttc
    25261 tgcatcatta tccattacat catgattcat atagccgtga atatgtgtat ctaccaaacc
    25321 aggagcaatc catttgccgc tttggtcaat gatgttgact tctccctcag gtagttcttt
    25381 ggtgtattca ccaaaaagtc catcggtaat ttcaagatag cctgcatgtt tgacacctga
    25441 cgctaaaaag aatttgtcag catagataaa agttctcatt ctattctctc cctttgtttt
    25501 tctacctcta aagtactctt atttagagaa gaagtcaaga atggtctaca ccatttacaa
    25561 attttttgaa atttgtttca cagcttgtgc taagccttca tagaatgctg ctctttctgg
    25621 ttcttcttta cgcttgtata ttaagctttt aagcacaaat aaatcattga ggtaatagta
    25681 gctattcttc tcttgagccc aatagattcc ttgattcacc tgttctaacg cttcgtcgat
    25741 ttcattttga tggaataaga aatttccata attataaaaa atttgcgaaa attcactttt
    25801 tgatcgttca tcagctgatt gatgcagcag ttggatgctt cgactcaaat agtagcgtgc
    25861 ttcagccgtt tttcctgcca caccatatat ttttccgata caactaatca gttgtatttc
    25921 tgtgtcagaa acatggattc tttctttttg gctagagtag cttaatgctt ctttcaaata
    25981 ataaagggac tgagagatat tttgacgcaa cgtgaattcg caaacaccga gataataata
    26041 atagcgttga aagtcagttg cttgatacag attttcagaa atatcagaag attttagtac
    26101 ttgttctaac ttccgataat cacgtttttg gaaacaacta tccaacagtt caaataattt
    26161 atcgtttgtc cgagtagttt ttcgagagag attcatcaca tgatcaatcg ttacccctag
    26221 ccgttgacaa atctgcatca tcaccaatac attcggcagt tcctcattat tttcgatccg
    26281 acttaataca ctttgtgaac aaatatcatg cgaaagcatt ttttgcgtca ttttttgttc
    26341 ttttcgaatc tctttaatga tcgttccaaa ggactcgatt aacgtttcca taagtatcct
    26401 ccccaaaata tgcgtattcg cattctattc gtattttact ttaggaagtc gcaaacttca
    26461 agaaaagact tgcaactaaa taatatactt tagttattta agaaaactcc actagaaaga
    26521 gagcctcagt gtgtttgtct gtggccctcc tactgaaaaa tttggttttt aaaatcattt
    26581 ttggcgatag gttgctagga attctctcat tttcttagct gtttgtttca aatcatccat
    26641 tttaggaaga taaacgattc gaaaatgatc tggctggttc caattaaatc caccaccatg
    26701 tacaagtaga atatgatgtt catgtaaaaa gtccaaaacg aatttttcat catccaaaat
    26761 attgaatcgc tttgtatcaa tttttgggaa tatataaaaa gcagcttttg gttttacggc
    26821 tgaaagacca ggaatatcat tgattgcatt atagatatat tctctttgtt catagatcct
    26881 tccgcctggc aggagcagct catcgacact ttgataaccg cctagcgcgg tttggataat
    26941 ttgctgagac aatacattgg aacacaaacg catggaggac aacatattca gcccttctat
    27001 atagcctttg acgtgtgact tatctccact taggaccatc cagcctactc gaaatcctgc
    27061 tacacgatga gatttggata gaccgttcag cgtaacaacg aacaaatcag gcgctaatgt
    27121 agcaatcgga atatgttcta gaccatccat cactaagcga tcatagattt cgtcggaata
    27181 aataatcagg ttattttgtc gagctacttc aacgatttct tctaataatt cttttggata
    27241 aagtgcccca gtaggattat taggattaat gataacaata gcttttgtcc gactcgttat
    27301 ttttgatttg atatcatcaa tatcaggata ccattctgcc tcttcatcgc aaatataatg
    27361 aacaggtgtg cctccagcta atgcaataga agccgtccac aacggatagt ccggcattgg
    27421 aaccagtact tcatctccat tgtccagcaa cccctgcata cacatcgtga tcaattcgct
    27481 cactccgttg ccggtgtaga tatcattgat cgtcacattc ggaaaatgtt tcagttggca
    27541 atattgctca attgcttttc tagcagaaaa gatccccttg gaatctgagt agccttctga
    27601 gtcacgggca ttcatgatca aatcacgaat gacttcgttt ggtgcttcaa atccaaaagg
    27661 tgcgggattt cccgtgttca attttaaaat actaatgccc tcttcttgca tccgctccgc
    27721 ttcttctaga acgggaccgc gaacatcgta actgacacct tctaatttgt tggatttttc
    27781 aaattctctc atcacttagc caatccctca tttcaaaaag ttagaacttt ccaaattttt
    27841 tgttatttac tatttcaata tcatactcca tcctagaaaa ttttgccact tatttaggaa
    27901 aaacgtaagt attaaaaaaa cacaaacaag cgttgagaac ttaattgtca tgatagtcat
    27961 tccatagatt cgctgcccaa tccattgcaa ttccacaaat gattcctagc ataaaaccaa
    28021 gcaaaaaacg tccgaatgca agtccaatca atacacctaa gatcattccc agtcctgaaa
    28081 attctccgtc atatttctcc atcttaataa gtcctcccat ttttgagctt gacaactatg
    28141 taaaacctcg ctatcattag tgtgttatta ttacgcagat gtgttggaat tggcagacaa
    28201 gcatgattga gggtcatgtg agcgttagct cgtgtgggtt cgagtcccat catctgcact
    28261 acaactcgtg aagttaattt tgcttcacga gtttttttgt ttgaacgttt ctttatgcca
    28321 ttatatacat atcactgtat cctttacaaa aagaacgtac aatcatgtaa acagcctgag
    28381 gtgagtatat gaaggtcgga aacaagctac tcgtagaaac ttgcaagtgt taaaacaatt
    28441 aatcgaaaaa cgagtaaaat ccctcaccta atactaaagc gagggatttt ttgtatatta
    28501 tctgtttatc accgttatta taaaggtaca aataccaagc acggttaatc caaattgcat
    28561 aaattcattt ttttgtacac tcatgctttt cattagctct ttaactgcca ttagtactaa
    28621 gatggcgata acaacataaa gtatatagta cgtccgcatt ccggaagccc atactgtggg
    28681 agaaaatccc ataattaacc ttgaacaaaa tcccattatc atcaaaataa ctaaaaaaat
    28741 agaagttttc ttattgtcaa aacttaaata aattccaaca attaaacata gtaataatgc
    28801 caagactact aaagtagcta tccaagtacc tggataaaaa agtgacagtt tcgttcctaa
    28861 ctctgtaaag agattattca ggttactact actccaaatc attgcccttt tatttccatt
    28921 gacgtaagta aagctttgac ccattgtatt accaaaataa ataatcaggt ttaagaaaaa
    28981 cggtaatgca gtcaatatac gcacataata gttttgacat ttcctatatg ttagaaggaa
    29041 aattaagaaa aatagcaata aaaataagat attcatgtct aaaaaaaacg gctttccaaa
    29101 tgatgaaata cctaaatcta acttattaag aaagttgaaa tttttatatt caggaaacca
    29161 tttattaatt tcatgtgcag cacgtaatgc attccctgga gttgtcaaag agaaaattaa
    29221 ttctgctaag cttattattg aaaatactga taacttataa ttatatcgtc cattgacaat
    29281 caaatataaa ctaactatac ttgttagaac aaaaaaacag gcgttgactt gttcttgatt
    29341 agttaaaaat atcaataaag gaatacttac ccaatatatt tttctattta tttcttctcc
    29401 tcttaagagt tgaaaaaacg gataaaaagt tagtaaacta aatgctactg gccactgata
    29461 gttcaaggtg gttgctctcc aaccggtttc ccccattact gtcaatggta ttaaacagta
    29521 tatagagcaa accaataata aatttttaac atttattttc tcaaaaacat
//
LOCUS       DACECY010000033        29021 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.034, whole genome shotgun sequence.
ACCESSION   DACECY010000033 DACECY010000000
VERSION     DACECY010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.034"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1026)
                     /locus_tag="IWO38_001562"
     CDS             complement(<1..1026)
                     /locus_tag="IWO38_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0313654.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNT"
     gene            complement(1108..1797)
                     /locus_tag="IWO38_001563"
     CDS             complement(1108..1797)
                     /locus_tag="IWO38_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0313655.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1903..2271)
                     /gene="rplL"
                     /locus_tag="IWO38_001564"
     CDS             complement(1903..2271)
                     /gene="rplL"
                     /locus_tag="IWO38_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ0313656.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2324..2827)
                     /locus_tag="IWO38_001565"
     CDS             complement(2324..2827)
                     /locus_tag="IWO38_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ0313657.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3153..3842)
                     /gene="rplA"
                     /locus_tag="IWO38_001566"
     CDS             complement(3153..3842)
                     /gene="rplA"
                     /locus_tag="IWO38_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ0313658.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(3946..4368)
                     /gene="rplK"
                     /locus_tag="IWO38_001567"
     CDS             complement(3946..4368)
                     /gene="rplK"
                     /locus_tag="IWO38_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ0313659.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4711..5379
                     /gene="sdaAB"
                     /locus_tag="IWO38_001568"
     CDS             4711..5379
                     /gene="sdaAB"
                     /locus_tag="IWO38_001568"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ0313660.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5392..6264
                     /gene="sdaAA"
                     /locus_tag="IWO38_001569"
     CDS             5392..6264
                     /gene="sdaAA"
                     /locus_tag="IWO38_001569"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ0313661.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6355..7374)
                     /locus_tag="IWO38_001570"
     CDS             complement(6355..7374)
                     /locus_tag="IWO38_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ0313662.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7547..8434)
                     /locus_tag="IWO38_001571"
     CDS             complement(7547..8434)
                     /locus_tag="IWO38_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0313663.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8511..9056)
                     /gene="nusG"
                     /locus_tag="IWO38_001572"
     CDS             complement(8511..9056)
                     /gene="nusG"
                     /locus_tag="IWO38_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ0313664.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9157..9327)
                     /gene="secE"
                     /locus_tag="IWO38_001573"
     CDS             complement(9157..9327)
                     /gene="secE"
                     /locus_tag="IWO38_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ0313665.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9351..9503)
                     /gene="rpmG"
                     /locus_tag="IWO38_001574"
     CDS             complement(9351..9503)
                     /gene="rpmG"
                     /locus_tag="IWO38_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0313666.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9665..10309
                     /locus_tag="IWO38_001575"
     CDS             9665..10309
                     /locus_tag="IWO38_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0313667.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10365..10868)
                     /locus_tag="IWO38_001576"
     CDS             complement(10365..10868)
                     /locus_tag="IWO38_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ0313668.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10881..10927)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11465..12430
                     /locus_tag="IWO38_001577"
     CDS             11465..12430
                     /locus_tag="IWO38_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ0313669.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12529..13701)
                     /locus_tag="IWO38_001578"
     CDS             complement(12529..13701)
                     /locus_tag="IWO38_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0313670.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13688..14833)
                     /locus_tag="IWO38_001579"
     CDS             complement(13688..14833)
                     /locus_tag="IWO38_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0313671.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14847..15779)
                     /locus_tag="IWO38_001580"
     CDS             complement(14847..15779)
                     /locus_tag="IWO38_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0313672.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15896..16975
                     /locus_tag="IWO38_001581"
     CDS             15896..16975
                     /locus_tag="IWO38_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0313673.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            16972..17601
                     /locus_tag="IWO38_001582"
     CDS             16972..17601
                     /locus_tag="IWO38_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0313674.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17711..17938)
                     /locus_tag="IWO38_001583"
     CDS             complement(17711..17938)
                     /locus_tag="IWO38_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0313675.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18041..20629)
                     /locus_tag="IWO38_001584"
     CDS             complement(18041..20629)
                     /locus_tag="IWO38_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ0313676.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20649..21308)
                     /locus_tag="IWO38_001585"
     CDS             complement(20649..21308)
                     /locus_tag="IWO38_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0313677.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21357..21860)
                     /gene="trmL"
                     /locus_tag="IWO38_001586"
     CDS             complement(21357..21860)
                     /gene="trmL"
                     /locus_tag="IWO38_001586"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ0313678.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22081..22947)
                     /locus_tag="IWO38_001587"
     CDS             complement(22081..22947)
                     /locus_tag="IWO38_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0313679.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23056..23697
                     /locus_tag="IWO38_001588"
     CDS             23056..23697
                     /locus_tag="IWO38_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ0313680.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23759..25120)
                     /gene="mgtE"
                     /locus_tag="IWO38_001589"
     CDS             complement(23759..25120)
                     /gene="mgtE"
                     /locus_tag="IWO38_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ0313681.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25138..26037)
                     /locus_tag="IWO38_001590"
     CDS             complement(25138..26037)
                     /locus_tag="IWO38_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0313682.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26039..26836)
                     /locus_tag="IWO38_001591"
     CDS             complement(26039..26836)
                     /locus_tag="IWO38_001591"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ0313683.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26814..27518)
                     /locus_tag="IWO38_001592"
     CDS             complement(26814..27518)
                     /locus_tag="IWO38_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ0313684.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27649..28224
                     /locus_tag="IWO38_001593"
     CDS             27649..28224
                     /locus_tag="IWO38_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ0313685.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28342..29019
                     /locus_tag="IWO38_001594"
     CDS             28342..29019
                     /locus_tag="IWO38_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ0313686.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8544 a         6145 c         5221 g         9111 t
ORIGIN      
        1 agtattattt cggttgcttg ctgcttcacc tagcccttgt actcgtgttt gattggcgac
       61 ttgaccaaat tgattcccga cgtctaataa aataatagct aaaagcaaaa tggccaaatg
      121 gcttccgcca accaataaag caataaagct tattgtttgc ataacaagtc ccatatacac
      181 gatttttctt tcagaataag aatctgccag tcgtcccacg ataggtgcag caactgctcc
      241 agacaaacca aaaatcgcta atatccctgc ttcgaatgtt ccccaattgt aggcgggact
      301 gctgatataa aaaatcaacg tagaccaaaa gatcgaaaat gttccaaaca tgaaaaatcc
      361 actgactgcc gcttctctta atagacgttg agaagcaact aaatgcggca aacttttaag
      421 tgaatcaagg tatgaaacag ctgtactact ttgctggaca gaacgcggca ttttccagta
      481 aagcagaagt gtaagaattc cgactgcaca taaagcaaat aaatagatca tccgccaaga
      541 aaaaagactg gcaacaatac cagataccgt ccgagaaagc aaaatcccca cgagtaaacc
      601 gctaagcaat gtacccatga ccttccctct tgcttttggt cccgctagta ccgcaccgta
      661 aggaatgatg atttgcggca cgatcgaaag taatcctatt aaaaaagaag cgcatgcaaa
      721 taacgtaaac gaaggcgcaa aaaaagcggc caacagagag acggcagata acgctaccat
      781 tcgaatgatc aattttggac gatccaccac gtcccctaaa ggaactaaaa acagtaaccc
      841 taacgcatag cctaattggg tcaacatcgt caatatcccg actgcacttg tgctaacaga
      901 taaactagct gctacttttg ttccaatcgg ctggatgtaa tacatattgg caacaacaac
      961 gccacaagtg attgataata gtaaaataag cgaagttgtt aaaggtgttt tttcattact
     1021 cttcattgat ttcccccatt tcctatctta gtttacaact tatggaaaag attggaaata
     1081 gaaaaataca actattcgcc acacttatta tttgtgcttt tgttttcctt gttttttctt
     1141 gcgatcttta taaatgaaga caactaaccc tactagcaca gtaacacttg caaccaacgt
     1201 gactgcagtt ttgttatatc cgtgaaacag agcttctcca ccacacgcat acaacacaga
     1261 cgaagggatg actccaatca cgatcgacaa tatgatttgc tgtagaggca atttcatcgc
     1321 atctgcagca aaattgacca tagaagatgg aataaccggt atcatatagc ctaacattac
     1381 acctattttt ggatgtttca tttgtcggat tgcttgcacc caatgattcg tttcggtttt
     1441 cttatctaaa aattttaagc gatgcataag gaaaattgca gtgatgttac caagagcatt
     1501 acccaaaatg ttgatgatcg cacctgtcag tggtccaaag ctcaaaccag caatgactcc
     1561 aactactgaa gtaggaatac ctggaacagc acacatcacc gctgtcaata cgatcaagag
     1621 tatcgatgca aaagtcccat gcgaacgaat atgtgcgagt agctctgcct tactcgctct
     1681 cggatttaaa aataattgga tgtctgtctg atattctaaa tacaaccgat agcccactac
     1741 cagtatcaga atgattccca acgagatcaa catcaatcgg attattttct ttttcatatt
     1801 tcctcctgaa aaaaatctat attcattaaa taatagttta acataaaaac aaaaaagcac
     1861 cgaaatccaa cataggatct cagtgctttt aaatagtaaa aattatttaa cagttacaga
     1921 tgcgccaact tcttcaagtt tagctttcaa ttcttcagct tcttctttag atacgccttc
     1981 tttaactggt gcaggagcgc catcaactac agctttagct tctttcaagc ctaaaccagt
     2041 tgcttcacga actgctttga taactttaac tttttggtct ccagctgcag ttaattctac
     2101 agtgaattct gtttgttctt cagcagcagc agcgccacca gcagcagcta cagctacagg
     2161 agcagcagca gatacgccaa attcttcttc aatagcttta actaggtcgt taagttctag
     2221 gatagttgct tcttttaact cagcaacgat gttttcaatg ttcaatgcca ttataatttc
     2281 ctccgtttaa ttgttctttt ttatttaatg ctttatgcgg caattaagct gcatctccat
     2341 cttctttgtc tgccactgct ttgacagcgt aagccacgtt gcggactggc gcttgtagta
     2401 cagatagcaa catagataga agtccttcgc gatttggcaa ttttgccaat gctgtgattt
     2461 cttccaatga agagacttta ccttcgatga taccgccttt gatttctaat gcttttgctt
     2521 ctttagcaaa ttcgtccatg attttcgctg gtgcgactac atcttcgtta ctgaatgcaa
     2581 cagctgtagg tccagtgaat acttcgtcca tgccttcaag gccagctttt ttagctgcac
     2641 gtgaaagaat agaatttttg ataactttca tttctacgtt agcatcacga agttgtttac
     2701 gtaaacgtgt tacttcatca actgttaatc cgcggtagtc aacgactact actgatgcag
     2761 ctgctgtaaa tttttcgctt acttcatcaa cgattgctgc tttttttgcg ataattgctt
     2821 cactcatttt tgagtttcac ctcctgaatt ttgatggaag agttttttgc actgaaaagg
     2881 caagtcactt ttatagttac tttgctcaac cagtattgtt catcatctct tcacttcgtt
     2941 catagtgatt cacaataaaa aactctatgc caccgcagac atagagggac tattgaatat
     3001 attcaataaa cgtcctcggt aggaaattaa ggcttgatgc cacctactgt cttcggtaca
     3061 agttaattat taacctcaac tagcttagca tgctaaacta gttgagtcaa gaattatttt
     3121 tcagttggat caatcaacat tctaatattt aattagaaag aagcttggtc aacatggata
     3181 ccaggtccaa atgttgttgt gatcgttaca tttttcatgt attgaccttt agctgctgat
     3241 ggtttagctt ttagcaatac atcatggatt gctgcaaagt tttcaactaa tttttcatcg
     3301 tcaaatgaaa cttttccgat tggtacatgg atatttccag ctttgtccac acggtaagtt
     3361 actttaccag ctttcacttc gtttactgct tttgttacgt ccattgtaac tgtaccagtt
     3421 ttagggtttg gcattaagcc ttttggtcct aatactcgtc ctagacgacc tacagtagcc
     3481 atcatatcag gtgttgcaac aactacatca aagtcaaacc atccgttttg gattttttga
     3541 accatgtcgt cgtcacctac gaaatctgca ccagctgctt cagcttcttt tgctttgtct
     3601 cctttagcaa aaactaaaac agtttgtgtt ttaccagttc cgtttggtaa tacaacagca
     3661 ccacggattt gttggtctgc ttttttaggg tctacgttca aacggtatgc aacttcaact
     3721 gttgcatcaa attttgcaat gtttgtttct tttgctaaag ctactgcttc ggcaacagga
     3781 taaactttac ttgaatcaac tttttttaat gcatcttgca tttttttgct ctttttagcc
     3841 attttggttc ctccttgatt gtggttataa cggttgtacc tcccacgtgt tctatatctt
     3901 tcaatataga gccaactgag aagctgctgt cttagggtgt atagattatt ctacagtgat
     3961 ccccatgctt cgtgcagttc cttcaaccat gcgcattgct gcttcaacgt tagctgcgtt
     4021 taggtcttcc atttttaatt cagcgatttc tcttacttga tcgctagaaa ctttcgcaac
     4081 tttatttttg tttggttcgc ctgaaccttt ttcgatttta gctgcttttt tcaatagaac
     4141 agcagctggc ggtgtttttg taacgaatgt gaatgaacgg tcttcgtata cagaaataac
     4201 aaccggaatg atcaaacctg cttgatcagc tgtacgagcg ttgaattctt ttgtgaatcc
     4261 catgatattg atacccgctt gacctagtgc aggacctact ggtggagctg gagttgcttt
     4321 acctgcagga atttgcaatt taacgatttt ttctactttc tttgccacga gacatacctc
     4381 cttaagtccg tgatgtggtt aattggagat gcatatttct cctcccacgt tttatctgat
     4441 ctgaaaaaat ccagacaaat aatgtgcaaa acaatgcaca ctggtacagt atagcaaatt
     4501 tatttgtttt ttcaagattt ttttctgcta tttttacatt ttgcttgaat aaacgctaaa
     4561 tttattctaa aaaaacaaca atttttcgtt ctcatttctt cttttatgtt acacttgcgc
     4621 tagcatggtt caacagatga aagcaaacaa taacacggtt ttttagaccc ttgtacaaag
     4681 attcctacat aacaaaatga ggtggagaaa atgaaagatt taaggtatag aagtgtcttt
     4741 gatattattg gacctgtgat gattggtcca agtagctctc acactgccgg agctgctaga
     4801 atcgggaaaa tcatacgtag tatatttggc gaacagcctg attctgtaga tatttatcta
     4861 tatgaatcat ttgcaaaaac ctatcggggg cacggaacag atattgcatt agttggcggc
     4921 ctgttaggga tggaacccga tgatgaacgt ttagctgatt ctttgaaatt ggcatatgaa
     4981 gccggtatgg aagtcttgtt tgttcctaag agtgaaaaag ccgaacatcc aaactctgta
     5041 aagatgcttc taaaaaaagg aacacgaaag ctttcagtca ctggcatttc gatcggcggt
     5101 ggcaatatcc agatttctga attgaatgga ttcaaaattt ctttaagcat gggtactcct
     5161 accttgatcg ttgtccacca agatgttcca ggtatgattg ctaaagtgac tagtatactt
     5221 tctgagacca atacgaatat cggcactatg acagtgacac gggaatcaaa aggtgaaaat
     5281 gccattatga taattgaagt agatgatcct caagtagaag aaacggtaaa aagactcaaa
     5341 cagctgccaa atatcgacag cgtaaattat ttcgattaag gagctaaaaa aatgttttta
     5401 tcgattgaag aattagtaga tcaagcaaaa gcttatccaa gtgtagcaga attgatgatt
     5461 gcagttgaaa tggaaatgag tggccgttcc cgtagtcaca tcatcgagat catggaaaaa
     5521 aacctctcag taatggaaca atcaatcaac gagggaaaag aaggtgttac ctccgtcaca
     5581 ggaattaccg gtggtgatgc aaaaaaatta gatgattatt taaatgaggc agacttttta
     5641 agtggagaaa caatcttaaa agctgtacgc aatgctgtag ctgttaatga agtcaatgca
     5701 aaaatggggt tgatttgtgc tacaccgact gccggaagcg cgggtgttgt tcctggtgtg
     5761 ctgatggctg tccgagatcg gcttgatttg acacatgaac agcaattgga attcttattt
     5821 accgctggcg ctttcgggtt agtcatcgca aacaacgctt ctatcagtgg tgcagaaggg
     5881 ggatgtcaag cagaagttgg ttctgcaagt gcaatggcag cagcagcttt ggtttgtgca
     5941 aaaaatggga ctcctcatca agcagcacaa gcagtagcca ttacattgaa aaatatgatg
     6001 ggactgattt gcgatcccgt tgcaggctta gttgaagtcc cgtgtgtcaa acgtaatgct
     6061 ttaggctctt ctcaagcttt tatctctgct gatatggcat tagcgggtat cgaaagtgtc
     6121 attccgccgg atgaagtcgt tgctgcaatg taccaagtcg gacgtcaaat gccgtcgatt
     6181 ttcaaagaaa cagcagaagg tggcttagct gttacgccaa ctgctaaacg acttgcggaa
     6241 gagattttaa ataagagggc gtgataaagc tccttaattt cgagtattaa gagattagtt
     6301 atcttcataa caaaaaacga agagaggact aattgccctt ctcttcgttt tttattaatc
     6361 catcaatttc gtatttttta cttcatcgat cgtttttgtt cctgcaagtt gcatcgtgat
     6421 cgacaattct ttgttcaggt gttcaaaaac agatgttact ccttctgccc cacctagatt
     6481 caacccataa atcactggtc ggccaatcgc aactaagtca gcaccacttg ccagagcttt
     6541 gaagacatgc tctcctcgac ggacaccaga gtcgaaaatc actggtacac gtttgttgac
     6601 tacttcggca atttttggca atacttcaaa ggacgcagga cctccatcta attgacgtcc
     6661 accatgattg gatacccaga taccatctgc ccctgcagag atagcgatta ctgcatcttc
     6721 tggcgattgt attcctttta cgataactgg aagatgggtg atttctttga ttgttttgat
     6781 gtcatctggt gtcaagcctt gttttgcagc tgcataaatt tctgcgattc cttttccttc
     6841 gccattacca cttgcacttt gttcgctata cgcagctaag ttaggcattg gtaatggaaa
     6901 ttggaattga ttgatgacgt cttcttcacg gtaaccaccg agcgttgaat ctgccgttaa
     6961 aatgatagct ttagcccctg cttctactgc tttgttcaaa atgaactcat tgaatctgtc
     7021 gtctttactc atatataatt ggaaaaactg cggtgcgcct ggcgctgcat cggctgcgtc
     7081 tttgattgtc gtatttgcat aagtgctgat ggaaaagatt gatccagcag cagctactcc
     7141 tttagctgta tcagcttccc ctttttcatg tgccaatcct tgggccgctg atggcgcttg
     7201 gataatcggt gtatctaatt caattccgaa aacagatgta tgcaaatcgg ctgagtcgat
     7261 tccgcgtaat atccgtggca tgattttctt tgtattaaaa gaagtcgtat tttctttcat
     7321 cgtccactca tcttcagaac caccgcgaat atagccaaaa gctcctgctt ccatgcgctc
     7381 ttttacacga ccttctagtt aggcaatgtt tacaatatcg attggatgtt cttcattact
     7441 tgtatgataa gtcatagtag atccttcttt catcttcttg attacatata aatcatacta
     7501 aacttacaaa aagtaagcaa acatttactc tcagatactt ttatcgctat tctttccaaa
     7561 taaatcgact tacaagctga tctacttctt ggttttcatg aagcatacta tgagaggcat
     7621 tttcacctgt cactactttt tcctctattt catttccgcg ttgcttcaac aacgcataga
     7681 ccgctaatgc actgtttaac ggtactgttc catcactcaa gtctgtttca tctagttgac
     7741 cagcaatcaa gaaaaatcga gtggttatag gaatattacc gatcatttgc tggtaatctt
     7801 gatagcggtt agaaacgaca gcaggcccct ttgctaactc atctgtcaga gactgcgcac
     7861 tatcatcgac aaaatcatta aaaggcgcgc caattgctgt taatgtctga acttgtggca
     7921 atgacgtatc ctgtccatac gttcctaaat agcgtagtgc actaactcct cccatggaat
     7981 gccccactat gttgacttta tctatgccgt agttttcttt cagatacaaa agacaagttt
     8041 tgatccattc tgcttgattc cactcgttat ttttattatc ttcaaacaat acttggacca
     8101 tcggattgtc tgcttttcca gacaaatctc ctgttgcttg tatttgtcca tctgttccta
     8161 ctgttaaaac caattctttt actgcttgct gatttttttc caaacgccgc aacatccctt
     8221 taaaggaatt ttctgtcccg ctgtagccgt ggaaaaaaag tgttggaata gtggtttttt
     8281 ctatcacttt actggattct gccgtttttt ctttatcaga aacttcagta gtatttatct
     8341 tcgtcgtctg gcaaccactt aacgcaaaca aactgataag gactaaagct gctgcagttg
     8401 cacgtttgca taaatttttg attggttctg tcatacacct cttcctttcc atatagaaaa
     8461 aagaaagact tgaaaggcca atcatatgat cgacatttca agtctcttta ttaatcgatt
     8521 ttatctactt gatcgaaatc aagttctgta ctggtttcac gtccaaacat atcgatgttg
     8581 actttcaatt tttctttttc tccgtcaact tctgttactt gtccttctaa tccagcaaat
     8641 gcgccttcga tgattttcac gacttcgcca acttctactt ctagatcatt ttgtcgcagg
     8701 ctcataccga tagaacgcaa gatatgattg acttcttctg gaagtaatgg tgctggtttg
     8761 cttccggctc cgtgtgaacc aacgaatcct gtcacaccag gtgtgttgcg gacaacatac
     8821 catgaagcgt ctgtcatgat catttcaaca agtacgtatc ctgggaatgt tttatgaacg
     8881 atttcttttt ctttcccatt tttgacttca gtttcttttt cttcaggaac aacgacacgg
     8941 aaaataaagt cggccatttt catactttgc gcacgagatt cgatatttgc ttttactttg
     9001 ttttcatatc ctgaatatgt atgcaagaca taccagtttt tttcaaaaga ttccattgaa
     9061 ggctcctcat ttcattgtaa aataaaaaaa cctcagtaca cccgaagttt ttctttcgct
     9121 tcattatagc actaaaagta cgttgtggct agcgagttat tttaaaatcc agccaaataa
     9181 gctttggatg gctgtatcca ttacgtaaaa catcaatccg aaaataatag aagtttcgat
     9241 gactactaac gtatctttac ggagttgttt tctgcttggc caagttactt gtttgatttc
     9301 atcttttacg ctttttagaa atttcatgag acaacctccc ttgctactgt ttatttcgtt
     9361 tctttatgta atgtatattg attacaatat ttacaaaact tattgatttc caaacgttcg
     9421 ccgctttttc cttcactgac tgattttgtg tagttgcgag acccacaaac ggaacaagcg
     9481 agtgctgctt ttttctttgc catttacttc tgcccccttg ctgagaatca gacttatact
     9541 tcataacagt atcacgtaac tttttacttg tcaatttgtg aaataaacta tctattgttt
     9601 tggataaaat tatgatatga ttaatttgaa attaagctaa aatgagacaa ttggaggtat
     9661 agcgatgcta ttaaaaacac ttgtatataa gaaaaaagat ctgacaaccg ttacagagac
     9721 tagtacatta gaagaagcat taaaaatttt agaaacttcc ggataccgtt gtgtgccgat
     9781 cttagattct tcaggtaata ttttccgtgg aaacatttac aaaatgcata tctatcgtca
     9841 taaagcaaat ggcggtgata tgacgcttcc tgtcactcat cttttgaaaa acgctacaaa
     9901 gttcatttac ctggatactt ctttcttcaa agtattcttt acgatcaaag agcttcctta
     9961 tattgctgtc cttgacagtg aaaatcattt ttatggtatt ttgacacata gtacgttgct
    10021 taatatgctc tctcaatctt ggagcgtaga acaaggaagc tatgtactga caattgcttc
    10081 cacaggaaag caaggagatt tggctgctat tgcaaaaatc atttcaaaac acagcagcat
    10141 tgccagctgt attactcttg atatcgaaaa agacgaatac attcgacgta cattgatcac
    10201 gctaccggct aacactgaag cgaaagtatg tgaaacgatc gtcaaccact tagagaaaaa
    10261 gaattataaa gtggtagaaa ttgaaaattt gcaaaaccaa gaagcataat caaaaagcga
    10321 aaaaagaaaa cagctaatct gctttctttt ttcgcttttt ataattagac acgcttttca
    10381 aaatcaagcg cgcgatctaa ataatacatc aatggtgctg aaacagccat gacgataaac
    10441 tcacttaatg caagcgtgcc ataggttgtc caaaatgctt ggccacctgt tggaactaac
    10501 atataagcaa tcagtcccat gctgatggta aagaagatcg tgttcaatac taaacgcatc
    10561 tttacatttt tcactttgtt ttgcaataaa gctgtcaggc ctaaagaaag caatgtttgg
    10621 ccgccaccaa aaaccacatc catgatacca aaaccaaaga aggcattata gacgatgacc
    10681 ccgcctaaaa ctccccaaag caatttacgg ttaaagacaa ctaaatgatt caaactttct
    10741 gaaatacgaa actggatcgg ccctgaggat acaggcgcaa ctaaaatagt taacgctaga
    10801 taaagagcca tgatgatgcc atttacaacg attacggata accgttgtct gcttttcgtt
    10861 ttttgcattt tctctcctct tttctcctag tttttttaac gcgggatggt tgatgaaccg
    10921 cggttatgtg aaacaaacaa taattataac agacttttca tttatttcaa tccgtcttat
    10981 ctgcttttaa aaaagcaaaa gcttaaacaa tgtaaacgtt tgctaaaaat cgtgggatat
    11041 ggtataaagt aagtgtaagg ggtcataaga attcccagta aagcagacga agtaggatct
    11101 gcaagaacag aattgttgtg aaacttaaat aaataaaaaa ggatgtggga atttgagcaa
    11161 cctcttacta gagaacggtc tagcaagcag ctgaagatct aagtggacat gccgaacctt
    11221 ggaacttctc cttacgtaag taatggatca tgcgcttgct aggctttttt gtgtttttcg
    11281 gtcttcggac ctttttcatt ttcctagact tctgctataa taaccaagaa ttaattttgt
    11341 aacagatttt tttgaaaaaa caagtttctt tcattcaaaa aatagaaaaa gcttgctaat
    11401 tattggttaa ccagcaaaca ggagtattaa aatatcttgt tttattgaat agcaggagga
    11461 aatcatgaaa ttccattatc atatattgat caatccatcg gcaggcagcg gaaatggcta
    11521 taaagtagca gaaagaattt taccggtatt aaaaaataag catattgact atacacttca
    11581 ttatagtgaa tacaaaggac acgatgctga gattgctgaa actttggcaa aagaaacatt
    11641 gattccttgg atagaagaag aacagaaaac aatcgatact ttcccccttt tgatcatcgt
    11701 aggaggtgac ggcaccttac atcaggtact tgatactttc tatcagatgg aagtagaatt
    11761 cccagttgct tatattccag caggttctgg aaatgatttt gctcgagggg caggccttcc
    11821 aaaaaatccc aaaaaaggac ttcagctgat tttagcagct caatcaccag aaaaagtcca
    11881 cataatggct tatgaagaaa aaataagtga aaaaaaagga atagctgtta ataatttcgg
    11941 tataggatta gatgcagcta tcgtccatgc gacaaatcat tcttccacaa aaaagcgatt
    12001 gaacaaatac aacttaggat cattttcgta tttattctct attctccgtg ctttattcac
    12061 tcaaaaagga tttccaattc tggtagatgc aggaggaaaa caattaagtt tttcaaacgc
    12121 atttttatgt acagcaacga agcatccttt ttttggcgga ggaatcgcga ttgttccaac
    12181 tgctgatgcg tctaaaccag tcattgattt tgtcgtggtc gaacgaatca atttctttaa
    12241 aatcttgtgg ctcattcttt tacttataca gaaaaaacaa ttgaaatcga aatatttcca
    12301 tcactacaca acaaacagat tacgtatcgt ttcaacaaca ccgcaacatg gtcaagaaga
    12361 tggagaagaa atggaaaaac gaccttttga tatcacaata accacaacta aacagttgtt
    12421 ttggtgttaa taaaagaggt tttgtcagaa gctacatctg gaacaccgtt tattcggaaa
    12481 aaaggactgt gacacgactt ttgtcacagt ccttttttta ctatgcagtt aattctttgc
    12541 ccaattgggt tgccatcgat agtttctttc tttacccaaa ataaattgga tcattgaaaa
    12601 caccgcagca aaagcaacta agacacaacc attgaaccaa aaagattctg caaatgtttg
    12661 attgtaactg agtgtttcac tgatcaagtt attattttga acaaaccaat atgttggtgt
    12721 gaatgccgct attttgctaa ccatttctgg caagtattct gctggtacaa acaccccacc
    12781 gataaagcaa ctgcctgtga taaaaatatc tttgatccca ccgctggttt ctgagttttt
    12841 taccaatgaa gtgatacaag ccgaaaagct aatgatcggc aataaaaaca agaaagtatt
    12901 taaaatatga taccataccg ttatatccca cgaggtgtgg atcactaaca gaaccattag
    12961 aagtaagaag ccactgaaca acagcagcga gtaactaatc aaactaaagg ttactttgcg
    13021 attcaagttt cgtttggtaa tgggcgcact ctggtttcgt ttactgatct ctttgcgatt
    13081 aaacgctaga ttgatgacgc tgaaaccgct aaaaatggat agaaacaacc cataagacaa
    13141 gaaattaaag gttgtgcccg tcaacagctt tttcatccgc tgactatatc ccgcacttag
    13201 ctcaacttct ccagtaaccg agagtgccat ttgtgtctgt tccatagctg catcgattgt
    13261 tttatcttct tcttgaaaca gaatcagtgt ttgcaaaaag gtgttcactt gctgtgtaac
    13321 aactgtttta gaaaaggcat ctgggcgact ctgtatcttg atcgtcgcca tctttcctac
    13381 ttcgacttgt tcggtaaagt ctgcaggcag atgtaagata atgtttacac gattaaaata
    13441 aagagcatca tcgatttctt tttgtgaggt tccatccaat gtaacgactg tttccttgtc
    13501 attcaaatat ttacgaaggc catcgactac aggcgactct tcttcactaa tcaaggcgat
    13561 tttcgcatct tgcagagagg tattttcaac atcaatattt tgcgcaaaaa agaatgtggc
    13621 aacaacagtg ataattacac ctatcagtaa attcgattta tttgctttaa gcaatgtcca
    13681 aatcgtttta taaagcgtca taacgcaccc ctctttccat ccaagcattt cctaacgcaa
    13741 aaaggaaaat aaatccgatc aaccatagaa gattggtata aaaactagcc aaatcagtgt
    13801 agaaataaag tttatacaaa ctttcgctga tcaagttgac gagatttaat tgaccaacaa
    13861 tcggaatatg aagatcaatc caatacttca cttgttccga ccccatcata ccagcaaaaa
    13921 agctcatcgc aatcgtcact gaaatcaaga tgctatcctt ttgcaccaga ttcatttttg
    13981 gtatcaaatt accaaccaat gtacctaaca aaatcgcaca caacgacccc agagcggtaa
    14041 caatcatcaa ccattgccaa cgttcaccaa agtctacttg atagataaaa cgaaagacag
    14101 ctaacataat ataaatctct gtcaaaacaa tcgtaaagct tgctgccaaa ttagcgattg
    14161 aaactaacga ttgctttcta ggaatcaagg acaagcgaat accatttgac gattgatttg
    14221 cttgctgatc ctgtacattg cggacccccc acatcgtccc gtagagaatc gccattccaa
    14281 tcagagtgaa gaaataaaaa cttttgaaac tcatattacg acctgcatga acttcttgaa
    14341 aagatacttc ttgttcaaaa acagtcatga tgggcgcgag atcaacgttc tccacttctg
    14401 ccactttact cgaaattata ccaatacgct gtaaaaattg atccatgacg tttttcatca
    14461 acgtctgact cattcctgct tgacccacat ggagcgaaac agatgaacca tcaatgagat
    14521 aaaaaccagc caaatcatct gcatttagct tttcattcgc ttctacttcc gatagttcct
    14581 gtacttcaaa taatggttta tcattgactt cgacttgttc taagactgtg gtaaaatttt
    14641 ccgaaagcgc tgtttgatcc gctttgatta caattccagt attaatcgtt tcaagattgc
    14701 caccttgatc aatctcgcca aaggccgcca taaacatcaa tcctaaaaga attggaaagg
    14761 ccaatgtcca aaacaacatg gatttatttc gcagcagtat ttttgtgcga tatttataaa
    14821 aatgccaaaa cattatttcc cctcctctag tctctcaaat ccttgccagt aatctctaaa
    14881 aagacatcat tcaaggacgc ttctttggtt gctatttttc cgtaactgat cttttcttct
    14941 tgtaagattg taagaatcgg caacaatact gtatttagtt cagtagttgt tacttggagc
    15001 tgaccttttt tataagcgat tccatcagaa gctattgttc gctgaatcgc tgacaacaca
    15061 ttttctggga gtaatgggat gtcgatcgtc aaacgatccg ccgtccgtac catatctttt
    15121 agagattctt tggttccttt agcaatcacc tgtccttgat ccatgatcac gatctggtcg
    15181 caaagaattt ctacttcttc catgtaatgt gttgtataga caatggttgc tccttgtcga
    15241 ttcagctcct tgatactttc taaaatttta ttccgacttt gggggtctac agctactgtc
    15301 ggctcatcta aaaaaattaa ccgcggcttg tgggcaatcc cacatgcgat gttcaaccga
    15361 cgtttcaagc caccactaag ttctcctgga cgaaacgaaa catatttttc taatcccact
    15421 aattgaatca cctcttcaat caaggctttc cttttctctt tttcggaaac atataaaccg
    15481 caaaaataat cgatattttc ttgcacactt agttcgtcaa agaccgcaac gtcttgagga
    15541 acgaccccaa tcattgcctt gacagcataa ttttcaggag tcattgttgt cccgaacaat
    15601 ttaatggttc ctttgtcata atttaacaaa gaaagcatac agttgatggc agtcgatttc
    15661 ccactgccgt ttggacctaa cagacctaag acttctcctt cttgtacttg cagatcaaaa
    15721 tgattcaatg ctacaaaatc tccatagcgt ttgactaatt tatcaatttc gataatcatc
    15781 atgaaaaact ccttccaaat ttcttgttga tctgactata ataaagtgca agaaaagaaa
    15841 ctagtgacga ttggctaaac cacacatgac atttgtcaca atgaaggagt ctgctatgca
    15901 taaatctcgg ctactcaacg aaactagctt tctactggtt atctcattgc ttttgctggt
    15961 aagtcaagga ttcgttgcta tgcaagttat ctatttattg atcgccgttt tattcactgg
    16021 tgtcagttat ttactgccta caaagccaaa ttggattttc caagttattc tatttctttt
    16081 atgtgccttt ttacctgatc ttgtcctgtt tctgccagcg acgatgcgag tgatcgcccc
    16141 tgaagaaagc caccttgatt acttgttttt gttaggtttt ttgttgattt cgacggttgg
    16201 taatcatccc cccatcaagg tcgcctttgt cgtaattttg ctgttttttt ccttttactt
    16261 acgaaaaaaa gatcaacgaa cgaccgatct tgagaaaaag tacattgtct ccaaagatac
    16321 tcattgggaa aaacaacaag agttatcgga acaaaataaa gcattggccc tgtcacaaga
    16381 aacacaatta gccttgcaaa ttgtggaaga acggaaccga attgcccgag acatccatga
    16441 caatgtcggt catctgctgt caagtgcgat tttacaaatt ggtgcgcttg aaatgatcaa
    16501 tcaagaacca aaactaaaag aaccattaga aaatttaagt gccaccgttc acactgggat
    16561 ggatcggatt cgtcaaagtg tccatgacct ccatgagaca tcgatctctt ttcaacactc
    16621 cttagagttt ctgatcgctg attttccttc ttctgttacg attgaaggaa acttgtttga
    16681 aggattgaat gaaacgcagc aaaactgctt cttaatggtc atcaaagaag ccttaaccaa
    16741 cagtatcaaa catagtcatg ccaaaaccgt tgttttacat tttcgaacgt tgccggcctt
    16801 ttatcgcttg caaatcacga atgatggcac cagcccacaa aaaaattctg ctcccggtat
    16861 tggtttgaaa acgatgcgtc aacgtgttca agaacaaaag ggacaagtcc atattttcca
    16921 agaaaaccag caatttcagt taacgatcat tttaccaagg gagggacacc aatgataaca
    16981 gtagtcatta ttgatgatga tccatttgtg acgaaatcgt tacaaaccat tttagaaagc
    17041 acgaaagaga tccgtgtgtt aggcattggt cattgtgcca aggatgcgct agtcttatac
    17101 gaaacccatc gtcccgatgt tttgctgacg gacatccgca tgcctaaaca aacgggcatc
    17161 gatgctgcca aagaaatttt ggctaaattt cccaaagcca tccttttgct tttgacgaca
    17221 ttcaaagatg aagaatacat ccgcgaagca ttttccatcg gtgtcaaagg gtatttaatc
    17281 aaacagaact tgcaagcgat tattccttct gtcaaatccg cttataatgg acaggccgta
    17341 tttggtaatg aaatcatcga gactttcact caaatgatga agaataaacc gacgattgat
    17401 agtcatgctt atgcttcttt tagtgaacgt gagcttgcaa tcatcaaaga agtagctgcc
    17461 gggaaaaaca ataaagaaat tgcagatgcc ttgtacttga gtgacggcac tgttcgcaat
    17521 tacatcagcc aattactaga aaagctagat ttacgggatc gcacgcaatt ggctatctat
    17581 ttttatcagc atttgcaata agtcttatct aacacaaaaa aacctgaatg atgctccttc
    17641 gcatcattca ggtttttttc acatcttcta ttcaattgtc ccgcaaacca tttcctcacg
    17701 attgaaagaa tcaaggattc gcagggctgc caggattcgt tggattgttc ggcgtgttag
    17761 gcggaaaaat aggcgccttg tctaacgttt tttctagttt tgattcttca cgatcgtctc
    17821 gttctacatc aggactttca ttcaatttga attctttttc catctcatct aactttttaa
    17881 agtcattgcc agattctttt gctaatttcg ctaatttatc ttcggtttct ttagccataa
    17941 agtaacgccc cccttttgat ttcagcttag catatcacac tgaaaaaaca aaagaaaacg
    18001 ctaaccaacg atcattttta gctatttctc atctttaaaa tcaagcattt tttgccggta
    18061 taaaatcgta gggagaactg ttttccatac caatcatcgg atctactaac tggttatcga
    18121 tcacttctgc tgtaagaacg aaatctttag aaacagcagg ttctacctct tctttctttt
    18181 cttctccaac aaatgaagaa aacagatctg gttccactgt ttccattact tggtttttcg
    18241 tttcattcgt cccactgtca tcttctacag gtagcttttc ttttacaacc cgtggctctt
    18301 ttgtatagtt cccatcttca aatggatcat ttccgaccaa agaatcttca taagctgcta
    18361 gtttggtccg gagagtgata ttcattttat cattatccat gatccttgat ttcaacgtag
    18421 tcatccggct tgctgattca ttcgctacac aagtgacata ggcttgctct tcccctaaca
    18481 aaatcaacag ttccttactg cgagtcactg ccgtgtagag caaattacgc tgaagcatcc
    18541 gctgatattg atggaccatg ggcaaaataa ccatcttgaa ttcactgcct tgtgatttat
    18601 ggattgagca acaataagaa agagtgattt tattccattc gtttctttta taagtgacct
    18661 catttgcatc aaactggata actaattcat ccactttatc ttcagaatct tttgccaaga
    18721 tgatacccgt gatttctccc atatctccat tgaatacatt caattcggga gtattgacta
    18781 attggaggac tttgtctcct atccgataaa cggtgtcgtt ccaatgtact tccttttttc
    18841 caggtttccc tggattgaat atctcctgca tcattttatt taacgcatcg atccctgcag
    18901 gtccccgata cattggtgct aatacttgga tatcttgtgc acggaatccc ttatttttcg
    18961 ctttttccac aattttagag atcaagggtt caatttgata agcatcactt cggaaatagg
    19021 aacgatcttt ttgattcttt gtgaaatctt ttggcagacg tccttctttg atctcatggg
    19081 caagaggaat aatactagaa ccatcacctt gccgataaat ctgattgagt tccattttgg
    19141 gaatttgttc gatttccaaa agatcatgga gtacttgacc aggaccgaca gagggaagct
    19201 gatctttgtc tcctactaag ataacttgca tattcgtcgg aatcgctttg aataatgtgt
    19261 tagccagcca tgtatcgacc atagacatct catcaacaat caaaagtccg ccttctaatt
    19321 ctttcgtact gactgaaggt gttttttccc gtccattcag ccccagaaga cgatggatcg
    19381 tactggcagg cagacctgtc gtttcgttca ttcgtttagc tgctcggcca gttggtgctg
    19441 caagtagaat aggaaaaatt tcttgggtat attttgaagg atccaattcc agtccgttca
    19501 actctgcaaa aagattgacg attccgttga tcacagtagt tttccccgta cctggtcctc
    19561 ctgttaaaat aaatagaggg gaacggattg cttcagcgat agcttgttct tgcgaaggtc
    19621 catactgtat gccgaatatt ttttcgatac gccgaatatt cttctgcact tctttttcag
    19681 gataatggat ttctttttta cgactgagaa ggcgctggat cgaattaccg atcccccatt
    19741 cagaaaagta caaactgttt tcgtaaagat tagtctcttc ttgttgtatc tttccttctt
    19801 caacaagttg gataattaca gtagcaacct gatcaggctg gatttcaatt gggcgacttg
    19861 tttctaaaat acggatcgtt tgctgaagca atgtctccgc cggtacgtat gtatctcctg
    19921 tttgaactgc atgctgaaaa atctgatgca agatagctgc tcgaattctt ctcggtgaat
    19981 ctgctgcaat accgatttgt tcggctagat tatctgcacg tttgaacccg attccttcta
    20041 tgtcttcgac tagctggtac ggattttctt cgatgatatc caaagactcg ttcttatagg
    20101 cctggtagat actgaaagct aactgactac cgaacccgta acgattcagt cccatgatca
    20161 cttgatccat cccgtggttt agccgaatcg tctcagcaag catttctctt tttggcttcg
    20221 tcaaaccgga gatttgtttt aataccgaag gatcagcaat gattttttcg atagcatctt
    20281 ctcccagcaa ttctacaatt ttttctgctg ttttcttccc gattcctggg aatttttcgc
    20341 tggaaagata cgctacgatc ccatttgcag ttgttggctt ttcttgctca tatctttcga
    20401 gtttaagctg ccttccgtat ttaggatgat ccactaattc tccataaaaa cgataagtct
    20461 cattttcttg aatatcgccg aaacttccgg tgactacaat ctctttttca cggtaatcag
    20521 ctgtggtttc actgatgtgt accaaaagca ctttgtaaaa attactagga ttttgaaaaa
    20581 aaatcgcggc aactgtacca acgaaataag gcatttcttc tggctgcatc ggaagccgcc
    20641 tccttcttct atagtaaagg ttctggtgct gtttcagttc ctaaaaaatc tgtttggttc
    20701 catactttca gttcatatgg caataatctg caaggctctt cagtctctaa aatcgtcaaa
    20761 ctactattga acaatccacc catttcacgc aactcactta gctcttttcc tgtcatccat
    20821 tgaatggctg cagttaaaga agctccatgt cctacgaata aaagtgggcc atctttatgt
    20881 cgggcaaccg catctgctac aacagtttcg atacgtgtga ttgccgattc aatcgtttct
    20941 ccgtgaaaag gagaaggatc atacagatct aaatgattac gcaaattcgg cagattatcc
    21001 ggatatttct ggtacatttc atcgatcgac tgaccttcta aagtacccag ccctagttct
    21061 cttagcttat ctgtataaaa aatctctacc ggacgagcaa gctctttgtt gataccttct
    21121 gctgtatcac gtgctcgctt tgctgtactg gaataaattg ctttaaaagg aacatatttt
    21181 acctttttcc caaatgcttt gatttcttcg taactttgtg gcaaaagcgg agaatcgcca
    21241 ttgctccctt gaaaacgtct ttcctgattc cattgtgtct tcccatgtcg ggtaaaatat
    21301 agttccatac gcagcaaact cctgtctttc ttttatttta ctctataata aactgtctaa
    21361 tccagcttgt cattttcata gtgatgcgtc aattctaaat tagggaatcc ttgctggcga
    21421 agtgcttcat acactatcat tgcagctgta ttcgataaat tcaatgagcg gacatgctca
    21481 tcattcatag ggatgcgtaa acacttttct tcgttttcac gcataaatgt ttctggcaaa
    21541 ccggttgttt cttttccaaa taaaaagaag tgctcttctc catcagcaaa attttcgtca
    21601 ctatatactt tatgggcaaa ttttgagatc aaatgtagtt tacgattacc taaaaatgtt
    21661 aaaaaagctt ctaaattact gtggtaagta atatttacat cattccaata atccaaccct
    21721 gcccgtttta gatgtttgtc atcagttgaa aaacctagtg gctcgattaa atgaagcgga
    21781 gtattcgtgg ctgcacacgt acgggcaata tttccagtgt ttgcagggat taatggttca
    21841 aataaaacga tatgattcat caggatcatt cctctcatat ttatgttcgt ttctattttc
    21901 tgacaatttg atttttgcat aaaaaaaaac gtatcaactc ggtgtcagtc actgcgagtc
    21961 gttacgttag tgaagctctt ctcactaact tttatcagaa caggaacctg ataaaaacca
    22021 atgaaaaaac aacttgttgc taatataaca ccctcaactt ttttttgcaa cctctactca
    22081 ttaaaaactg ctagaaaaac gttttctctt agttcctttt aataattcta tgtcaattgt
    22141 aatcttcttc aaaggattct tatataagtc tgctttgatt cgctcttcta cctgccattc
    22201 taaaggtgac atggccaaaa gatctttttg attttcctct ggtatctcaa attcataggt
    22261 tacacgctga cgttcagtta atttcattgc ttcagcaaac ttggctacta cacgttcatt
    22321 ggaataactc tgtttttcag gttgattcgg ataaaaagcc tgacgtagtt cctttaaata
    22381 attttcttgt ggcaccacct tcaacacttc tccatcttct gagagtacac gttggaattc
    22441 ttggtaatga gaaggcgaaa agatatttaa aaccgcgttg aatgtttgat cagcaaacgg
    22501 caaattggtc aaatcagcta cacaccaaaa tgcttcggca gattgttctg tagctgcgta
    22561 gactccttct tttgaaatat caaatccaac agcattttga atctttttca attttgaaag
    22621 ttcattcaag aagctacctt ctccgcagcc aacatctaat agcgagactt cttcaggtaa
    22681 atgctgcgca atagtctcaa taacgggctg atacatgcca gattggatca ttctacgcct
    22741 tggaacgaac attccttgat cgtattctgt tttaaccgca tgggataaaa aataaagtgt
    22801 tccttttttg gaaaggtcaa aacggtgatt atttttgcat atcaagccat tatcagactt
    22861 ttttacttct tcttggcaaa ccggacagcg gaacagctga tgattttctg ccaaaaactg
    22921 gcgtcctcgg tcaatttttt ttaacatagt tccccctcat ttctgtttta tcttaccgtt
    22981 agatgctcac tttggcaact gctaaaaata tgatacaatg cctagaagaa agtgaaagat
    23041 aaaggagaag attagatgat caaagaattt tgcgctgaaa actacacatc tattcctcta
    23101 gcaattgcta gaggagccga tcggatcgaa ttgtgtgaca acttagcggt cggtggcaca
    23161 accccgagta ctggtgtgat cgaagaaacg ttaagttatg ctggggaaaa agatatcccc
    23221 gtaatgacga ttattcgtcc tagagggggc gacttcgtct ataacgatat cgaattgaaa
    23281 atcatgcata ctgatttgat tgaagcaaag aaattaggga cggatggcgt agtatttggt
    23341 tgtcttactc cttcaggctg gctagacgaa gaggccttag aactattgat cgaaacagca
    23401 gaagggttgc agatcacctt ccatatggct tttgacagta ttcctgaaga tcggcaattc
    23461 gaagcaatcg attggctggt agaacatggt gttgaccgta tcttgactca tggcggtcct
    23521 gctgacaaaa ctattgtaga aaatttccca cggcttaaag aattgattgc ctatacagat
    23581 ggccgcttaa tcattttgcc tggtggtggt gtcactgcag agaatgcagc acatgtagct
    23641 aaagaattga acgtaagcga agtacacgga accaaaatcg tggatcttca gttataatct
    23701 tataaacaaa aaaacatttg caacaggcat aatgcttatt gcaaatgttt ttgttttttt
    23761 acataaaata gcccataaat aaagaagcaa tattgaaata aatcagtaca ctcgtcaaat
    23821 cgctcaaagt cgtgataaag ggaccgcttg ctactgctgg atcaaatcct agacgatcca
    23881 ttagcatcgg gattaaacta cctgcaagat tcgccactgt gattgcaaat agcatagcca
    23941 tccctatgac aaaccccaag ataaaattat gtttccaaaa acctacgatt agaaaaatcg
    24001 tgatccccgt aacagctcct gtgacaacgc cggtcaagac ttcagccaaa acaagtcgaa
    24061 caaatccatg atctttctca tcagaaatcg caagtcgacg aacagccaca gctagcgact
    24121 gggttccagc attaccggca gttcctgtaa tcagcgaaat gaagactgct aaaatactcg
    24181 cttcacttac caagtcttca tagtgactga ttaaagaagc ggtcgacata cctaaaaaca
    24241 acagagtgat cagccaaggc aaacgtcgag acgctgcctt tactggattt tcattaattt
    24301 cttccacatt gaccccggca agtcctgaat aatcgctagc agcttcatca tcaataacgt
    24361 cgatgatatc atcgactgtg acgattccta acaaatgatc atcataatcg gtaacaggaa
    24421 gagctaagaa gtcataatct ctaaatgttt gtgccacatc ttcttggtcg tcgcctacat
    24481 gaactgaaag cactcgttcg ctcatcagat cagaaatcat tgcgtcgtca tcattcacga
    24541 ttaggtcgcg cagagaaata acccctacta gctgaccttc ttgattaacg acatagatgt
    24601 aatagattgt ttcagctaca tctgcttcgt tcttcagcac gtacatcgca gagcgaacag
    24661 tttgattggc tacgatcgaa acaaattcag ttgtcatgat cgcgccagcc gtttcatcct
    24721 cgtaatggag aagttccttg atttcactgg catcgtctgc cgacatcaaa cttaaatatt
    24781 tagcaatttg ctttttatcc agcgtattaa gcaagtcaac agcattatct gtatacatct
    24841 cagaaagcat atcagctgca tagctaggac gcatttcatc taggtattct ttcatatgtt
    24901 cgttgtcttc ttcaatcaca tcgaacatat ccgctaattc tttaggcgac agataggaat
    24961 agatcagctg cctgttctct tctgtaagag attggtagaa ttgcccctgc tcatagatat
    25021 gcatctctaa aaaactgttt ctgaatgcct gaatattatt ttcccttaaa tcttgagtca
    25081 actgggcaaa tctttcctct aattcttgat tttcatccaa accaatctca cctcgcctta
    25141 ttcttcaaac cgtctcacaa ctgcctccat gtctgcggct aaaggctgct tgaactccag
    25201 caactcttct gtaaatggat ggtaaaaacg cagatcataa caatgaaggg cttgtctttt
    25261 tattcccaga tccattcgcc cgccatacat atcatcaccg agcagggcac agccaattgc
    25321 ggaaaaatga actcttattt gatgtgtcct tcctgtatgc agctggatat caactaacgc
    25381 gatttctgtt agtcgcttct ctatccagta ctcagtctgt gccgaacgtc cttcatcaga
    25441 gaccattcgc ttcaatagcg aagaataatc tcgggcgatc ggcaaatcga tatgtccatg
    25501 tgccgataaa tctgaaagcc ttccactgac taaggcctga tattttttca caaacttctt
    25561 ttcacgaagt tgcatatcta attttgcgtg cgcaaatcca tgctttgcaa aaagcatcaa
    25621 gccagatgta tcccgatcta gtcgcgtcac cacatggatt acttggtttt cgtaaccttg
    25681 ttttttataa taagccttta cacgattagc cattgtccca tttggatggt attgggcagg
    25741 aatagacgaa acgcctgctg gtttatttac taccagaacg tgttcatctt catacacgat
    25801 atcgatcggt gtttcatcca aaagtacggt ttcatgttct ccttcagcag gaatcacaat
    25861 ggttacctta tcatccttag ctagagaaaa caagacattt tctacttgat cattaacttt
    25921 gatttgaccg ccttgaaatt ttattttcgc taacagaccc tttgagatcc ctttttcttt
    25981 taaaaaatat tttacttgct gagaatgatc tttctcataa tcccatgtaa actgcatatc
    26041 aatcctcact aataaaggca tctttcacac gatgccaaaa atgcatatgt cgataagaag
    26101 caaaatggat ccgttcttct gctattcgat aataaattga gcggatattt gcttttgcga
    26161 cattcaattg atcaaccgtt acaagataat cttctgtatt ttccaatttt atttctagcc
    26221 attcatcatg ggcaatcacg atcggagaac ccaaagtacg aaacactcga ttattcagtg
    26281 aagcaatctc tgccaactga aaggcattga tactaggatg gatcacagca ccgccgacac
    26341 ttttattata tgctgtcgaa ccagtaggcg tagaaatcgt caatccatct cctctaaaac
    26401 tttcaaacag ctcgtttttg atgtatacat cggctaccat cgtacgattg gcccgtttaa
    26461 tcgttgattc attcagtgct aaaaaatgct ggtccggtgt ttcatccaaa taactgatcc
    26521 gaacatccag caagggataa ctaacacttt gttcccggtt cgttcgtaag ctgtctacta
    26581 gttcttccag ctcgtaatcc cgccaatccg tgtaaaaccc taaatgacct gtatgcacgc
    26641 ccaagaaacg cacatcattc agacaatgac tgtagtgatg gaacgccgac aataatgtgc
    26701 cgtctccgcc gactgagatg accaattctg gctcatgatc atctcgttga atccctgctt
    26761 gttctaaaag tgcgttcaaa cgttctgtca cttctatagt ctttgcttct ttattatgaa
    26821 cgatcgctac tttcatcccc tgcctcctct ctttcgcttt ctttcttcaa aagcgttcga
    26881 tagtagacat catctgattt tcctcttcca tgggagaata accgctgtgc ttcttggatt
    26941 tcttcacgga tactggacat ctcttcatcc aactgatagg cagcttctgc cgcacgctga
    27001 agtcgttcac tcatttcttc tggaaaaacg ccttgatatt tataattcag cgaatgctca
    27061 atcgttgccc agaaattcat cgcaagagtc cgaatctgga tttccgccaa aattttcttc
    27121 tcaccgctga tcagctgtac aggatactcg atcaccagat gataagaacg gtagccactt
    27181 gcttttttat ttgtgatgta atctcgttct tggagaactt tcatgtcttt tcttttacgg
    27241 atgatctcaa caacttgatg gatatcttct acaaactgac acatgatcct taacccggca
    27301 atatcagaca tttcttcttc taaacgtccc attggaatat gccgtaattg tgattttgtt
    27361 acaatactat cgactggctt gactctccca gtgacaaatt caatcgggac atgtcgattt
    27421 tgttcacgga actgtttgcg gatcccccgc agtttcactt tcaattctga tacagtttgt
    27481 tcatatggtg ctaaaaatgc ttcccaatct cgttccatag ctgatacctc tacttcatta
    27541 acattcattg tttattctac catatatgca aagttttttc gtactcttga tttatgcatt
    27601 gaccaaaaaa agaaaattag gcaaaataga caaaaaggag tcgatcaaat gacacaaatg
    27661 cttgaaatcg aatataaatc tatgctgaca aagcaagagt acgagatatt catcgctcac
    27721 tatcaattaa cagataaaga tttccgtgta cagactaata tttatttcga taccgttgat
    27781 gaacagctga agaaacaaaa ttgtggccta cggatccgtt tcgttggtca tcaagctgaa
    27841 tatacattaa aaacacctgc tgaaaaaggg cgtctcgaaa caacggatac ctttataaca
    27901 gaagaagcag aagcatttat cttcgaaaaa cgtctgcctc gttcaggcgc agtttttcaa
    27961 aaactttctg atttgaatat cgatcctgct tcattaatcc aaaccggaaa gcttacaaca
    28021 aaacgagctg aattccctat tgaagaagga cttttagcta ttgatgaaag ctggggagac
    28081 aatctacacg attttgaact ggaactagag gtaggggatg catctgtggg aaaaattgct
    28141 tttataaatt ttttaaaacg tttttcttta ccttatcgtc ctgctaagaa caagatccaa
    28201 cgtatgcttg aagctaaaaa ttagaaacct ttcattttca caaggcactt gagttcattt
    28261 ttttactttt ttctgttaag ttataggatg tgaaagaatc tagtcactag ttgtatagaa
    28321 tctattttgg gggagattca tatgatcgaa atctatttat ttgttaatcc tttaggggaa
    28381 tactgtttcg aaatggaaca acagttatta caatttatcg aagaagagta cgggacacca
    28441 tcaaaagaaa agatgcaatt tcgctttctt cctttggtca atttgcagac aattggggat
    28501 gtcatgcaac aaaaaggaat ttctcagaac aatcttgagg aacgtaatca cttattctca
    28561 atgacttatt ctgcagcatt ggactgcaaa gcggcgcagt ttcaaggaaa gaaaaaaggc
    28621 cgccgttttt tgatcaaatt gcaagaagca gtcggctgta atgacatacc atattctcaa
    28681 gaacttgctg aatccatatt tgaagaaatc ggcggcgatt tgaatatgtt taaagaagat
    28741 cgtcagtctg acttcgttaa agaagtattc ttttctgatc aaaaaattgc tcgggaaatg
    28801 ggcatcacaa aacacccttc tgcagtcgtt tacaattatg cctgcgaccg tgattttggt
    28861 gttttagtag aagtaaaagg tacaagtagc ctttggagaa tcacagagct gtgtaaaaca
    28921 acagataact catatcaagt ttttaatggt gaacatttac atacaaatga ttctcagttc
    28981 cattcaagaa ctagccattt acacttgctt tctaattaaa a
//
LOCUS       DACECY010000034        28793 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.035, whole genome shotgun sequence.
ACCESSION   DACECY010000034 DACECY010000000
VERSION     DACECY010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.035"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(56..643)
                     /locus_tag="IWO38_001595"
     CDS             complement(56..643)
                     /locus_tag="IWO38_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313687.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            818..2140
                     /locus_tag="IWO38_001596"
     CDS             818..2140
                     /locus_tag="IWO38_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0313688.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2295..3071
                     /locus_tag="IWO38_001597"
     CDS             2295..3071
                     /locus_tag="IWO38_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ0313689.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3064..3633
                     /gene="rnmV"
                     /locus_tag="IWO38_001598"
     CDS             3064..3633
                     /gene="rnmV"
                     /locus_tag="IWO38_001598"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ0313690.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            3626..4510
                     /gene="rsmA"
                     /locus_tag="IWO38_001599"
     CDS             3626..4510
                     /gene="rsmA"
                     /locus_tag="IWO38_001599"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ0313691.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            4961..5590
                     /locus_tag="IWO38_001600"
     CDS             4961..5590
                     /locus_tag="IWO38_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313692.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(5714..6823)
                     /gene="ugpC"
                     /locus_tag="IWO38_001601"
     CDS             complement(5714..6823)
                     /gene="ugpC"
                     /locus_tag="IWO38_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ0313693.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7032..7454
                     /gene="mgsA"
                     /locus_tag="IWO38_001602"
     CDS             7032..7454
                     /gene="mgsA"
                     /locus_tag="IWO38_001602"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ0313694.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            7777..8313
                     /locus_tag="IWO38_001603"
     CDS             7777..8313
                     /locus_tag="IWO38_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0313695.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(8353..10116)
                     /locus_tag="IWO38_001604"
     CDS             complement(8353..10116)
                     /locus_tag="IWO38_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0313696.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(10119..11834)
                     /locus_tag="IWO38_001605"
     CDS             complement(10119..11834)
                     /locus_tag="IWO38_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0313697.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(12613..12837)
                     /locus_tag="IWO38_001606"
     CDS             complement(12613..12837)
                     /locus_tag="IWO38_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313698.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            13037..15805
                     /locus_tag="IWO38_001607"
     CDS             13037..15805
                     /locus_tag="IWO38_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0313699.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            15886..16305
                     /locus_tag="IWO38_001608"
     CDS             15886..16305
                     /locus_tag="IWO38_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ0313700.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            16317..16787
                     /locus_tag="IWO38_001609"
     CDS             16317..16787
                     /locus_tag="IWO38_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0313701.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            16804..17583
                     /locus_tag="IWO38_001610"
     CDS             16804..17583
                     /locus_tag="IWO38_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0313702.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            17570..18391
                     /locus_tag="IWO38_001611"
     CDS             17570..18391
                     /locus_tag="IWO38_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0313703.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            18408..19457
                     /locus_tag="IWO38_001612"
     CDS             18408..19457
                     /locus_tag="IWO38_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0313704.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            19470..20474
                     /locus_tag="IWO38_001613"
     CDS             19470..20474
                     /locus_tag="IWO38_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0313705.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            21107..22126
                     /locus_tag="IWO38_001614"
     CDS             21107..22126
                     /locus_tag="IWO38_001614"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ0313706.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            22169..23050
                     /gene="galU"
                     /locus_tag="IWO38_001615"
     CDS             22169..23050
                     /gene="galU"
                     /locus_tag="IWO38_001615"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ0313707.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            23350..23727
                     /locus_tag="IWO38_001616"
     CDS             23350..23727
                     /locus_tag="IWO38_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313708.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            24333..24947
                     /locus_tag="IWO38_001617"
     CDS             24333..24947
                     /locus_tag="IWO38_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0313709.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            25720..27459
                     /gene="yfcC"
                     /locus_tag="IWO38_001618"
     CDS             25720..27459
                     /gene="yfcC"
                     /locus_tag="IWO38_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ0313710.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            27616..>28793
                     /gene="iadA"
                     /locus_tag="IWO38_001619"
     CDS             27616..>28793
                     /gene="iadA"
                     /locus_tag="IWO38_001619"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ0313711.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     "
BASE COUNT         9330 a         4938 c         6001 g         8524 t
ORIGIN      
        1 aacaatcatc ggctttgact tcgatgattg ttttctttat tttgtaaaaa tcctatcact
       61 tgattttatg gtaacctttt atttttgtct tcaaacggct tggtaagaga gcagtaagca
      121 gtgcaaatgg gatcgaaaag acataccaga aaatcgtacg tccgatacca taatgttgat
      181 agagctttgt caacatcacg tgtaacaaga tcaaccctaa aagcaaggag ccagctataa
      241 accaatgatt atcccaatgc atgacttcac taagtgccat cactccaaaa ggatagaaat
      301 aaaaatagat cccgactaag gtcatccgcc agttctttgt aaatgttccg atattcaata
      361 aaaagaaaat cgctgaaagc aaaataccga taggcggaaa aagcccaacc aaaaaggaat
      421 acaacatccc ccacaagatc atcacaggac ctttgatcaa tgcaacgatt gcgatgaaca
      481 aagtcagcaa tattagtttt tgtgataacg ttgcaacagt cattaatcca aataaaatta
      541 atgtgaacca atgccaatgg gactgctgtt gttctttacg ggaaaaatcg acccggttgg
      601 ataaataatc tgctaatttc ggataagaac gcttaaaatc cataaactgt tcctccttcg
      661 tggaaaaggt aggtttagta tacgaaaaat aaaaaagaaa tacatccgtc ttaagaggga
      721 gtttttcact gaaagtggag aaaaaagaat ggtaaattaa aatattctca gttatactga
      781 aaattatacc aaatttcaaa gaagaaagag gatgggaatg aaaggcaaac taaaacgaga
      841 aatcaatctg tttggcgcat tagctaccgt tatgggaacc gtgatcggtg caggcgtctt
      901 cttcaaaact gctgctgtta ctgctagtac tcagtctaca agtttgacat tgctagcttg
      961 gcttttgggt ggtttcttaa cgatctgtgc tggattgaca gtggctgaat tagctacagc
     1021 aatcccggaa actggtggag ctgtaaaata tattgaagct gcgtatggaa aattgcccag
     1081 ctttttatta ggatgggcgc aaagcttgat ttactttcct gctaatattg ctgcattatc
     1141 gattatcttt gcgacacaat tgacgaactt attacaatta tctaccgatt atcttttgtt
     1201 gattgcagtg atcaccgctg tttctgtgac agggttgaat ttgttaggga caaaagtcgg
     1261 tgcttctgta caatcagtga cgttgattgt taaattaata ccaattgctg tgattgtcat
     1321 atggggatta ttgaccccag gacaaggaac tgtccagtta tttccaatcg aagctggaaa
     1381 agatgttact tttgtcgaag gattgagcag tgcgttgtta gcaacgttgt ttgcttatga
     1441 cggctggtta ggtgtcggtg caatggcagg ggaaatgaaa cggccggaaa aagatttgcc
     1501 taaagcaatt attcttggtt tgagttttgt aacagtcgtt tatctattga tcaattttgt
     1561 ttttttgaaa actttgccga ttgatcatct tgccggtaat ttgaatgcag cttctgaagc
     1621 atcagacgtc atttttggag gaatcggtgg aaagctggtc actattggga tcttgatctc
     1681 ggtatacggt gcattgaacg gttatacact aactgggatc cgcgtgcctt atgcgatggc
     1741 tttagaagac gagctacctt tcagtaaaca gctgacaaag ctttcaaaga agtttacggt
     1801 tccttatgtt cctgcggttt tccagttagc agtagcttgt attatgatga gccttggttc
     1861 tttcgacttt ttaacagaca tgttgatttt tgtgatgtgg ctattcagtt tactgatttt
     1921 tatcggtgtc tttattttaa gaaaaaaagc gccggaattg ccacgtccat ataaagtacc
     1981 actttatcca atcgttccga ttattgcgat tctcggtgct attttcattt taggtatgac
     2041 gatgatgacg caaacgaaat tagcattgat cggtataggt gttaccttga taggtatccc
     2101 agtctattat caaaaaaaga aaaaacgctc agaagagtaa aatatttcaa caacagacag
     2161 aaaaagtacg aactaggtcc tctagatctt cgtgcttttt ctatttcaat aaaacaagtt
     2221 cttctatttt gatttttgct acttctgcaa gtagtgctta tgtgctaaag taagaatgaa
     2281 aaggagagat aaacatgata tttgattctc atacccattt aaatgctgaa cagttcaatg
     2341 aagatattcc agaaacgatt cagcgagcac aagaaatggg ggtaacgaaa atggcggtcg
     2401 tgggcttcga tacgccaacg attgaaaagt ctttgcaatt aagccatgac tatccaaata
     2461 tatacagtat tatcggctgg catccgacag aggctggcag ttatacgaaa gaaatcgaaa
     2521 ataaattaca agaacaattg actttaccaa aggttgtagc attaggggaa attggcttgg
     2581 attatcattg gatggaagac ccgaaagaag tccaagataa agtatttcgc agacagattg
     2641 ctatcgcaaa agaaatgaac ctaccaatca gtattcatac tagagaagca cttgaagaca
     2701 cgtatcaaat cctaaaagaa gaagatgttc gagatatcgg tggaatcatg catagtttca
     2761 gtggtgatta tgaatgggca aaacgatttc ttgacttagg gatgcatatt tcatttagcg
     2821 gtgtcgtcac tttcaaaaaa gcattagacg tccaagaggc tgcagcaaat gtaccaatcg
     2881 aacgattact tgtggaaaca gatgcccctt atctggcacc agttcctttt agaggaaagc
     2941 gaaatgaacc aggctatact cgttatacag tagaaaagat tgctgaactt cggaatcttc
     3001 cgacagaaga agtggctgcc caaacatgga caaatgcgca ccgattattt ggtctaagcg
     3061 aacatggata agatgaagat ccaagagatt atagtcgttg aaggaaaaga cgatacaaga
     3121 aggctgcggg aagtagttga tgcagatacg atagaaacaa tagggtctgc aatcaatgaa
     3181 gagatcctga tgcaaattga acatgcccaa gagactcgtg gagtgatcgt ttttaccgat
     3241 cctgattttt caggagaaaa aataagaaaa atcattatgg aagctgttcc tcaggcaaaa
     3301 catgcttttt tgccgcgcac acaagctaaa ccaaagaaaa agggaagcct tggtgtcgag
     3361 catgcaagtg atgctgctat tttagaagca ttgaaaaagg tcgtgacgcc agacatttcc
     3421 caaacggctg tttctgaaat cactagacaa gaccttttag actatggttt aatagctggg
     3481 gcacaagcaa aagaaaaacg agaaaaacta ggtgacgagt tgcgcatcgg ttacacgaac
     3541 ggcaaacaat tggaaaaacg tttgaagatg tttcgtatta cgccgaaaga actcgcccaa
     3601 gcaatgaaaa tagtggagga acaacatgag tgaatatcgt gaaattgcga caccctctag
     3661 aacaaaagag attttattga aacatggttt ttcatttaaa aaaagtttag gacaaaattt
     3721 tctgactgaa ccaaatattt taaggaaaat cgtagaaact gcgcaaatag atgaacatac
     3781 gaacgtgatc gaagttggac caggaatcgg tgcattgacg gaacaattag ccaagcatgc
     3841 aaaacaagtc gtagcatttg agatcgatga tcgtttgatc cctgtattgg ctgatacgat
     3901 gcaaccctat gacaatgtga ctattgtaca tcaagatatt ttaaagaccg atttgtcaac
     3961 agctgttaga gaaacatttc atgaggagct tccgttaaaa gtcgtagcga atttacctta
     4021 ctatattacg acaccgatca tgatgcattt tcttgaatca gatttggttg tggatgaact
     4081 agtcgtcatg atgcaaaaag aagtagctga tcgtatctct gctgaaccag gaacgaaagc
     4141 ttatggttca ttatcgattg ctgttcaata ttatatggaa gccagtttag cattcatcgt
     4201 accaaaaaca gtatttgtcc ctcagccaaa tgtagattca gcgattttga aactgacaag
     4261 aagagatata ccggcagtcg aagtgacaga cgaaaaagca tttttccgat tgacaaaagc
     4321 ggcatttcag caaagaagaa aaacgctttg gaataacttg cagcattcgt atggcaaaga
     4381 tgaccagaca aaagcatggt tagcaaaaag cttggaaaca gctggaatcg atccaaaacg
     4441 tcgtggggag actttgtcat tacaagaatt cgccgcatta agcaatgcga tgtcagaaaa
     4501 tatgcagtaa aaaaagaggt tgtgacagaa gttgtgaaaa agctgtcctg catcaagtaa
     4561 taagaacagc caaacaaaaa tagttcctca tgtttttcgt ttgtttgggc ttattaccgc
     4621 agatgcaagc ttttgaacac cgtttaagaa agaggttgtg ggcctgccgt ttaactctaa
     4681 gtcacaagga acaatttcaa aaaacagctt ctcatgtttt tgagttagtt ggtcttatta
     4741 cttgatgcaa gcttttgacc actattatgc cgaacaagga gatgtgacac aaattttgtc
     4801 acatctccta tttttgtttc ttgatcaaaa aattcttata cttgatactc acactcattt
     4861 tgtatggatt tagaaaagaa tatgtgtctt gacagttatt agtaagtgag ttatactaag
     4921 tcgacattta tgtgtcaaga catattagga gagagaaaaa atgagaaaaa attcagttcg
     4981 aaaactaacg atttctgctt tattgattgg gatgggggtc attatcccaa tggtcatgcc
     5041 taaaatcatg attccaccag cttcctttac tttggcgtct catgtgccat tatttattgc
     5101 gatgtttttt acaccaggag ttgcagtggc tgttgcgcta ggtacgacat ttggtttctt
     5161 tttaacaacg ccggttatca tcgcattgcg agcattgtca catcttgtgt ttgcgttgat
     5221 tggggcttgg tacttgcaaa agcatccgag tatcgtttta aaaaatggac agtttacttt
     5281 tgctaattgg cgtttccaag ggttcaattt tgtgatcggt gtgattcatt ctttagcaga
     5341 aatgctagta gtcagcatct tttactttat tggtaatatg cctgaaacat attattctca
     5401 aggatatttt tatacagtat tcatcttgat ggggattggt gggcttatcc acagtttagt
     5461 tgattataat attgcttatt ttatagcagg aacactcagc aaatattttg atatccccgt
     5521 atttacggca gcaaaaaaag aaaatcggaa agagtcgatt caagggtacg cgaagaaaac
     5581 gacatattag acatgatctc tcaaaaaaaa aactctcatc tagtaatgga taataaatcc
     5641 tctaagatga gagttttttg tcctaaaaag agctgcacca acatcttctg gcacagctct
     5701 aacagggggc aatttatgga agtttgatga cttgttctgt ttctttatca aagaagtggc
     5761 ctttgctgat gttgaaggca aggtcgacta tttcgcctgg tttatggaaa tctcttgcat
     5821 ctacttttga gatgaactca gtagaaccag tttttgtgta aagcatcgtt tcagctccaa
     5881 gtaattcgga aacgacaact tctgatttca ctacggcttc aggtgaagct tcaagggcta
     5941 cttgttcact gtggatgtct tctggacgaa taccgaagat aacttctttt ccttcataac
     6001 ctttttcaac taatactttg ttttttcctt caggaatacg aagcttaagc ccatgtccat
     6061 ctgtgataac accatttttc aatgtcacat taaagaagtt catggcagga gatccaataa
     6121 agccggcaac gaacatattg acgggtgtat cataaacttc tttcggtgta ccgatttgtt
     6181 ggataaatcc gtctttcatg ataacgattc ggtcagccat agtcattgct tctgtctgat
     6241 cgtgagtaac atagatggtc gttgtttcaa gacgctggtg aagtttggca atctcagcac
     6301 gcatggctac acgtaattta gcatctaaat ttgataatgg ctcatccatt aagaagactt
     6361 ttgcatcccg aacgattgcc cgtcccaatg ccacacgttg gcgttgtcca ccagataaag
     6421 cagctggttt tcgtttcaag tattctgtca gacctaagat ctctgctgca ttgtccacac
     6481 gttttttgat gtctgcttta tcatatttac gtaatttcaa gccaaaagcc atattatcaa
     6541 aaacagtcat atgaggatac aatgcgtagt tttggaaaac catggcaata tcacggtctt
     6601 taggtgccac gtcattcatg acttttccgc cgatatttag ttctccttca gagatatctt
     6661 ccagtcccgc gatcattcgc aaagtagttg actttccaca accggaagga ccaacaaaaa
     6721 cgataaattc acggtctttg attgatagat taaaatcagt tacagaataa ttttctgcat
     6781 tgtcatattt tttatatacg tttttcagag ccatttctac cataatacgt tccacacttt
     6841 cttttttagt tttattaact atttaaagta taaatgaaag cgttaactaa cttcaatgtc
     6901 atgttgaaca agaatttttt cttttttttg tcaatgtgct agttttaagc tttaataaat
     6961 atccataaga aataaaaact gatacactgg agtaaataag tatgttaaca atatcaaaag
     7021 gagtgtctct tatgaaaatc gctttgatcg ctcatgaccg aaaaaaacct atgatggtga
     7081 aattagcaac agcgtataaa acaatattgg aaaaacatga actttttgct accgggacga
     7141 ctgggcagag gatcacggaa gcaaccggct tgccgatcca tcggtttaaa tccggaccgt
     7201 taggcgggga tcagcaaatt ggagccatga tttcggagga tcagttagat ttggtgattt
     7261 ttcttcgaga cccgctggct gcacagcccc atgaaccaga tgtaacagca cttatccgtt
     7321 tatctgatgt ctatgagatt ccgttagcta cgaatatcgg aacagctgag gtgctactaa
     7381 gaggactgga agccggcttc ctagattttc gaagaatcgt ccatgaaata gacaagcatc
     7441 cactttcatt ttgatggata agcgtatgga aaaataacgt ttaatgttcg cctatatgta
     7501 gctataaaag ctaaacaaac tgataatatg cgatataata cgaacgtatt ggataatata
     7561 aacaataata ttagtttttc gtatttgcag taaatgtgaa aatatgctat agtttctgtg
     7621 ggaaaagatc ccaaaaataa tacagagtag aaaataaagc gttaagtgct aagaggatgg
     7681 gatgttgcct cttggacgaa ggacgtaatg ttcgcggttt tattttcgca ttcgctgcat
     7741 gatacgatgt agcagctctt aaaggagatg ctaaaaatga agaaacaccg gttagacgca
     7801 cgtatgattg ctattatggg cttattgatt gcattgatgg tcactttgtc ccgactggta
     7861 gcaatcgaga ccccatttat taaaatcagt gtcacattta ttccacaagt gatcatgggc
     7921 attctttttg gtcctttttg gtcagggata ggcgcagttt tagctgattt agtaggaatg
     7981 gccctcttct cgaaatctgc attctttatt ggatttacat tgaatgcatt tattgaagga
     8041 gcaatctacg gattcttttt ctatcgaaaa gagatcactt ggaaaaatgc cattctggct
     8101 acgcttagtg tcacgctgat catcaactta tttttgacac cattatggct tgcgttgatg
     8161 taccatgtgc cattgttcag ctgggtcgtt tgggcgcccc gcctgttaaa aacagtaata
     8221 tggttaccga ttcaatcaat tgccatttat tacgtaggac gtagtattcc ttacaaaaaa
     8281 attttaagaa gtttagcgat ccacgctaaa taaaaaacaa gcagtcttcc catcccagaa
     8341 gactgcttgt ttttattgtg ctgtgaattg tgcttgatag agccgtcggt aataaccatc
     8401 tttttgaagc agctcatcat gggtgcccat ttcgacaata gctccctgat ccatcactaa
     8461 tatctggtcc gcatttttga ttgtggacag gcgatgggca atcacaaaac tggtttttcc
     8521 ttccatcatt gctaagaagg cttgttggat ttttttctct gtcaatgtat cgactgagct
     8581 ggtagcttca tctaagatca acataggggg attactgatc atcgttcgag caatcgtcaa
     8641 cagttggcgt tgtccatctg atatctttaa cccttgaccg ccaatcttag tatctaattt
     8701 ttccggcaaa cgttctacaa attcatacat ataagaggct tttagggcat cattgatttc
     8761 tgcgtcactt gcttccggat tcccaaaacg aagattttct cgcaatgtac tgtcaaatag
     8821 ccaagtatct tgtaacacca ttccaaaact tttacgtaat gtatcacgtt ggatagagcg
     8881 gatgtcatgg tcatcgactg taattgttcc ttttgttgtg tcataaaaac gcatcagcaa
     8941 attaacaagt gtggattttc cggcccctgt ttttcctact atagcaatgg tttgacctgg
     9001 ataagcagta aaattgaaat cttcgatcaa acgctgcttg gggtcataag aaaaagaaac
     9061 atgatcaaaa gagacttttc ctttgatatt tttgggttcc aaggcatagg catctggcaa
     9121 atcaggcttt tcttctggct gatcgatcaa atcaaatgct cgttctagac cagaaaaagc
     9181 ggtttgcagt tgtgtcgtga ttccagaaag ttcgataaat ggtttagaaa actgacttga
     9241 gtagatcgta aagctggaaa tcacaccaac agtgattgac gaaccactct tcaatgccaa
     9301 taggccgcca actaaaccaa tcgataaata agcaaggtga tctacaaaac gagaagacgg
     9361 gtttgtcaag gaagaagaaa attgtgcccg ttgtcctttt tcatatagat cagcatttag
     9421 cagttcaaat tgctgttgac tagcttcttc ttgctgaaaa gctttcacga ttttctggtt
     9481 accgattcgt tcggtcacaa agcccgagat atccccaata atcctttgct gtctatcgaa
     9541 atttgtttgt gaagcacgag caacgatcca actcacaagg aaaataattg gtgtagaaca
     9601 aagaacgacc aatgtcagaa gtggacttag acgcatcata aaaataaacg aaagaatgat
     9661 gattgccact ccagaaaata attgattgaa ggccgcagaa attgcaatgg aaatattgtc
     9721 catatcattt gtaaatcgac tgacgatatt tccgtgggag ttttgatcat aataacgaag
     9781 tggcaatcga ttcagatgag caaatgcatc ttttcttaat tttgcaacgg ataagtaagc
     9841 taaccgattg cttaacactt ggatgagcca ttgactgact acgttgatta aaacgatacc
     9901 accgaataga ataagtaaat ggaacagtcg attgaagcgg acctgtcctt ctccaatcat
     9961 ttgatcgata ctttgaccaa tttgtaacgt catcacaact gttgcaatcc cattgatgat
    10021 tcctagtatc acagctagcc aaatgtcttt tgcataggca gtaaggtaag gaatgaatcg
    10081 gcggagcgtc tgcaaggata atttttttac tttcataact attcttcctc ctgtgatgcg
    10141 acgatttcct gatattctgc tgattgagca agtaattctt catgggtacc ctgagcagca
    10201 atcttacctt gatccatcac taagatatga tgtgcttctt gaatagagcg gacacgttgg
    10261 gagataataa tcactgtact gccaagataa gtctgtagcg ccttacgcaa atccaagtct
    10321 gtttgataat ccaacgcact taaagagtca tctaaaatca gtaatgctgg cttggcaatc
    10381 aaagctcttg caatcgtcag tcgctgtttt tgtccgccag agaaattttt cccattttcc
    10441 atgacttttg tatctaaacc atttggaagc tgacggacaa attcttctgc ctgagcgatt
    10501 ttcagagctt cccaacattc tttttctgtg gcgtctttct taccccattg aaggttttca
    10561 cgaatagtcc cggtaaatag tacagaagtt tggggaacaa gagcaatttg ctgacggaga
    10621 tgatccagct gccaagaacg aacatccatt ccgtccatta taactgtacc ggaactgaca
    10681 tcatagaaac gcggaatcaa ttgtgtcaaa gtcgttttcc cgcttccggt tggaccaatg
    10741 attccaagga ctgtatttcg cttcagctga aatgtaatat tttggagaga aagaccagaa
    10801 tcaggagtat aacggaagtc aacatggtca aaagcgataa ttgcttcaga agaaaaatcg
    10861 ggagtagtac tgacctcagc ttcaagaatc gaattttctg tgtctagcac ctctttcacc
    10921 cgatttcctg aagcttcggc acgtgtgaag atcacgacga ggtttgaaac aacgatcagt
    10981 gccaaaagca tttgattcat ataattgatc aaagcaagaa cttgtccttg ttgcaagcta
    11041 ccaatattta ctttgatccc gccaactgtc agcaaacaga tgatcccgac attcatgatc
    11101 aaggttgtag caggtgataa tagggcagag atattggaaa cacgtaaata attgttcgct
    11161 agatcatttg tgcttttatc gaacttctct gtttcttttt ttgtctgcgc aaatgcgcgg
    11221 atgacacgaa caccgctaag attctgacta acgtgttcat tgagctgatc tagtttttct
    11281 tgcacttttt ggtagagagg aacggttttt ttgatgataa aaaataaaat gattgagaaa
    11341 ataggcaata atgctaagaa aaacagacct acttcccagt cgatcacaaa agccatgacc
    11401 actgacccga tacttaaaaa cggtgctctg atcaataaac gaatgaccat tgctaaagct
    11461 agctgaagct gattgatgtc gtttgtcatt cgggtgatca aggtatctgt tccaaaatga
    11521 ttcaactcgt tgtgagagaa agtattgatt tttttgatta gctgattacg taactctgta
    11581 ccaaatcctt gtgaagctac tgaagcatag tattggcaaa tgatagcaca aatcagacca
    11641 atcacagaca tagcgaccat ccataagccc atttcaatga tttttcctcg gtcattcatt
    11701 tttagtccat tatcgatcaa ggaagccatc attaaaggca ggacaagttc gaagacagct
    11761 tcaagaaatt tgaagaacgg gcccaaaatg atttgcttac gatagtcttt tgcataacgt
    11821 aataactgaa tcatatgatt cctcctatta tttatttgtc cacaattgaa aaaacaagta
    11881 ctaccacatt gtagcttagg aaatttagaa tgaaaagaga acaagccttt tcataaatga
    11941 aagttttatt atgaagaagc tttagaaatt gaatagacta ttttttttaa aaaaaagaaa
    12001 atgagaaaac atgccagtct cttcattcat aggaacgtct attttactta atttttctat
    12061 aaatgaattc tgcataaaaa atatatcaag taaatgttta tagatgaaac tctatttact
    12121 tcttttttaa tttgttcttg caatcttttt tgctacgtaa tttttgtaaa aaagtgggtt
    12181 gagagaaatc taattgattt tcaaagaagg agtatgaaaa tgagaaaaat atttattcgt
    12241 ttattgtggg tactatctta gaggaataaa taatagtgga gaaaactatg gtttggcgca
    12301 gattctatca ggtttgatgg aacgaagaca acctcccgac atcaagtcaa aagctacttg
    12361 agattttgat ctgaaaataa aaaaagaaaa caaggtagga acatcaatgt ttctaccttg
    12421 ttttcgtaat caatgggaaa aaactaagag tggtgtacgt aatcaataaa aaggatggtg
    12481 acaacagtac ctctttactc tctgaaagca tcataactcg tttttcctca ttatgccaat
    12541 aaattgttgt tataccacat ataaggattt gtaatagcta aaaacagaca ggaaagttga
    12601 aatctgtgaa tcttattttg tatggaagga tttatagaac gcaaaaatca agataaagat
    12661 gaataaaaat cctgtttcgg ctatatattg cgtatctacg ccaataaaac gagtttgttc
    12721 tgtcatagcc atataaagca aaggcaaaaa tacaactaca gcgagataag atccgatgat
    12781 ttctcgaaaa gttttccagg aagtatgttt cctgttttta tatttatccg atttcattga
    12841 atagtcatag cctccttatt ctttcatatg ttgaaattcc agtataaaaa ctggaaggaa
    12901 ctgtacgttt tttagaataa aaattataag ggataagtag gctgctgtct ataataagat
    12961 atatttttca attctttatt aaagatttta ttgacaaaat gaatccgttt tctatgatgg
    13021 aggaggggtg aataagttgt tgaagaagga aattttatgt tacttagaga accagacagc
    13081 ctttttcgat ttagaacata tgaacgaggt ctttacagca caaaggttag ctgatcattt
    13141 tggggtaaaa agaaacacag tcagtcatta tttgaaccag ttaaatgatg agcaactact
    13201 agtcaaaatc aattctcgac cagtgattta tttccacaaa gaagcttttg aaaaacaatt
    13261 ttttaagtta cgcacgaatt tttatttttc tatcgatgat ctcaaagagg aacagccttt
    13321 tttcgcgcaa ccaaaagact tattctcttt tatgatcgga tacgacgcca gtttgaaaga
    13381 aagtatcgaa caaatcaaaa cagctcttta ctatccagat ggcggattac cacttttgat
    13441 tacaggcgaa agtggcacag gtaaaagtta tttagttaat ttggtctatc agtattgtct
    13501 gcttcatgat ctgctagaag attctgcacc attcatcact tttaattgtg cacaatatgc
    13561 agataatcct gaacttttga ctagcaattt gtttggacat gttaagggag cgtacactgg
    13621 tgcagaagaa aataaaaaag gggcttttga agcagcagat ggtgggatct tgtttttaga
    13681 cgaggtacat cgcttaacac cagaaggtca ggaaaaactg ttcacctatc ttgatcaagg
    13741 tgtgatttat cgtatgggag aacctaattt gtcacgccgg atcaaaacac gtctcttttt
    13801 tgctacgact gaagagatta ctagtcattt cttaacaact ttcattcgaa gaatacctat
    13861 ccgaatcgaa ctacctgcac ttaatcagcg tagtcgtaat gaacggctag aactgattta
    13921 ctcttttttt ctcgaagaac aaagaaaaat ggggagccct ttgtcagtca gtggaccagc
    13981 tttgtcactt ttaacaagtc agaaattatc cggaaacata ggagagctga aaaatatcgt
    14041 caaagtttca gcagcaaaag catatgctga acagcgtgaa cagcctgaaa tccatgtcac
    14101 gatccatcat ttgcaaaaag aactacttgc gcagcctatt actaaaaata cagcacagca
    14161 aggaatctat attacgcaag accagacctt agagcaatta atggagaagc aacagcccga
    14221 acagcagcga atcgttaaaa gttttgagca aatcctaatt gatttcaaga aaaataactg
    14281 tctgctatat tcttgcgaag gaaaattaaa gcaagaagtg attcaattat ttgatttttt
    14341 gctgtttgag accagtagac aagaaaaaca cgagctgctg gtttattgga cacaatctat
    14401 ccgagagacc tttcgacaaa tggaaactgc ctatcaaatc aaattcgatg gaaacagtgt
    14461 ctatgccttg agttactatc tctatcagcg aagcactatc aaatggttac cggaagaaca
    14521 tgatattatc caactaatca aagaattaga acaacaagtt tctcagtctt atccatctag
    14581 ttatcactat gtccaacgaa ttctggaatt aagtaagatc aaattagata ttgaagacac
    14641 aagtatggat cggattttac tgacgatcta tctaaaaaaa gcgaaatggt caaaagaaaa
    14701 acgactgcca aaagcgatga ttgctgctca cggttatgca actgctagca gtatggcaaa
    14761 tgtagtaaat cgtttattac aagaggatct atttgaatca tttgatatgc cactagatgt
    14821 cacaccacag cagattgctt cagaaatcct tgattattgc gaaaacagcg atgtttcaaa
    14881 tggattgatc atattagtag acatgggatc tctgaaagaa atccatcagt tttttaaaaa
    14941 acagttatcc gtaccgcttt tgattttgaa taatgtgacg acacctttag caatcacagt
    15001 aggggaatgc ctgcaaaaaa atgtatcatt agaagagata gcagaagaga ctgttcgaca
    15061 gatccagcca gaatggcgac tgctctatcc agaggaaaac aaacccaaag cactcattac
    15121 tacttgcttc actggaatcg gtacggcggt ccacttaagt gaactgctag aaaaaagctt
    15181 acctacggct tgtcagttga aaatcatccc atatgaatat cagcaattaa aagataagaa
    15241 aaacagtgac ccattatttt ccatatacga agtcgtagga atgattggca ccacagatcc
    15301 tgatatcacc aatatcgact atctttcatt agaagacctc atctcggggg aaaagatgtc
    15361 tgtactagct gaatggttgg acactactat gaatgcttca gagaaggaaa tattcaatca
    15421 gcgcatcatt cgtaattttt cattggaaaa agtactggat tccgtgacta ttctagatac
    15481 agagaaggtg atgggagaaa tcgatcattt tatgagagaa ttagaggtgc agttgaatcg
    15541 aagcatcagc aatcccaaaa aattagcgtt gtatgtccat gtgagctgtc tcattgaacg
    15601 attgatccga caaatgccaa tcgaaaccta tcaaggatta gagaaatgga agcagtgtca
    15661 aaaagaaggg ttatctgcta ttaaatctgc gtttagtgtc attgaagagc attatagtgt
    15721 catgatcccc aattctgaga ttgcctatat ctatgatatt ttgagtgaag atacagagtt
    15781 tctctcgata gaagaagaat tttgaacata agcaagttgt tgccactaat tttggcatgc
    15841 aacttgcttt tttagtatgt gtaagtgaaa agaggagatg aaaaagtgga tagagaaatt
    15901 atcttagctt cccatggaaa atttgcttca gggatattag attcattaga gctgatctat
    15961 gggaaaaatc attctattac tgtattcgat tgctatactg atgagaattt tgatttagcg
    16021 gaaacagtaa ggcagctttt tatgaggtac aaagataaag aaatcattgt tctgacggat
    16081 ctttttggcg gaagtgtcaa caacgaattc ttacaatata tcaatcggga tcatgtctat
    16141 ctcgttgccg gattgaattt gccactattg attgagctag tatctcggtt ggacgctgaa
    16201 gtagagacaa ctgttttgat ccagcaggtg ctaactgatt caaaaaaaat gatccagttt
    16261 tgtaacgagc ggctcaatca gaaaatagaa gaagacgaat tttaggagga agaaaaatga
    16321 ttgtattgac aagagtcgac catcggttat tacacggaca agtagctttt tcatggacac
    16381 aaacaatagg cgcagactgt attttgattg ctaatgatga tgtacctacg aatgagatac
    16441 gaaagacaac gatcaaatta gccaagccac aaggagtcaa actagtgatc aaatcaattg
    16501 atgactcgat tgccgcgttg aaaagcgggg tgacagataa atataagtta ttcatcgtag
    16561 tggaatcgat cgaggatgca tataagctgg cagaagcgta tccagaaatc aaaaccatca
    16621 acttaggagg gatcaaggca aaacctggta ctcgcagcat cggaaaagcg gtaaatattt
    16681 taccagaaga ggaggaacgc ttgaaacgat taattgacaa acaaatagaa gtagaaatcc
    16741 gtcaagtacc agcagataaa aaagtggatg taagagaagt tttgtaagaa aggaagaacc
    16801 tcaatgattg tacaagcagt cttattagga attgttgcat ttgtcgcaca atcagaatat
    16861 gcactaggga catcgttgct ttctcgtccg attgtgacgg ggctgtttac aggaatcgtg
    16921 ttaggtgatg taaaaactgg gatcattatg ggcgcaactt tagagttagc atttatcgga
    16981 tcgttctcag taggtgcttc gattccacct gatgttgtga ccggtgggat tttagggacg
    17041 gcttttgcta ttacagctgg agccggtaca gaaaccgcgc ttttactagg tttgccgatt
    17101 gctacgttga cattgatatt gaaaaatatt tacttagggc ttttgattcc aattatgaac
    17161 cataaagcag acaattatgc agaagaagga aattataaag gagtcgagca tatgcacttg
    17221 cttgccggta tcggtctttc cttgatgctt ggtgttgtgg taatggtttc ttatatggtg
    17281 ggaagcaata cgatcggaaa tctattaaat atgattcctg acttcgttca aaaggggttg
    17341 tcagtagcta cagggttgat tccagctctt ggttttgcga tgcttgctcg tttgctgatc
    17401 aacaaacaag ttgcaccata ttttttctta ggttttgcta tagcttctta cttagagatt
    17461 cctgtgaccg gagtagctat ttttggtgcg attttagcag ttgtggtagt caatattatg
    17521 aatgtccgtc aactaccact tcaaacaact gcaggggagg tagaagaaga tgaagaattc
    17581 tgagatcaaa aacagcaaaa taacgaaaaa agagctaaat cacgtatttt ggcgttcttt
    17641 tcaaatggag ttttcttgga attatgaacg tcagatgaat atggcttatg tttatgcaat
    17701 gattccgatt ttgaaaaagt tatatcagtc gaaagaagaa atggcagcag ctttgaaacg
    17761 gcatctagag ttttttaaca cgaccccaca tatcgttaca ctgattttag gaatcacggc
    17821 tgcaatggag gaagaaaata aggatgatcc tgaatttgat gtgactgcga tcgatagtat
    17881 caaaacatct ttgatgggac ctcttgcagg gttaggagat tctttctttt ggggaacttt
    17941 gcgattgatt gccacaggtg tgggtacctc tttagccatg caaggaaata ttttaggccc
    18001 tatcttgttc attttgattt tcaatattcc gcacattctg tttcgctatc tagcaacagg
    18061 ttggggatat cgcttaggaa cgggtttttt gaaaaagatc caggcaaacg gtatgatgga
    18121 aagtttgaca ttaggcgcgt ctattattgg actgatggtc gttggagcaa tgactgccac
    18181 gatgatcacc atcaatattc ctttaaaaat cggttcagga gaaaatgctg tgactgttca
    18241 aagtattttt gatgatatcg ttccaaatat cttatgtctt ggagcatttg gcatagtgtt
    18301 ttatctattg aagaaagaag tgaaaccatt aacgatcttg atcggactag ctttctttgg
    18361 aatttttggt tcgtggatag ggatttttta aaaaaggagc agagaaaatg tttacgatgc
    18421 aagattatat ctatgaagaa aaggaagtac taagtacgat cttgaaaaag aatgattttt
    18481 cgacaagaga acatatgaaa aaaacagtga atttactgat cttagcaacg ggttcatctt
    18541 ataacgcttg tttagcagcg aagccagcat tggaatctta tggtgatctt actgtcgata
    18601 ttcaagaacc tttttatttt ttcaattatg gaaaactttc tccttcaatc gatactgtga
    18661 ttgctgtgtc acaaagcggg aaaagtgctt ctactgttga agcagtaaaa atgatacaaa
    18721 aacaagggtt acctgttgtt gctatcacaa atgacgtgca aagtccactt gctttagaag
    18781 ctgcacaaat tattgattta ggtgccgggg tagaatcagt aggcttcgtt acaaaaggct
    18841 attctgcgac tgttttacag ttactactct tagggatagg aattggaata agtaaaaata
    18901 aaatcagcaa aaaaatagaa caagactaca tgcaacagtt gcgcaaaata atcaaccatt
    18961 taccagcgat tattcaaaaa acggaagaat tttttgatgg atatcaaagt ttatttcgtt
    19021 tagcacagcg tttcgtcacc attggatatg gtccaaactg gggaacagct aaagaagcag
    19081 agacgaaatt gactgaaaca atccgcgttc cgtcacaagg atttgagtta gaagcttata
    19141 tgcacggccc atacctggag gcagatgctt cgcatttgct attctttatt gaaggggaaa
    19201 gtgtaaataa ggaacgttcg caaaagttgc aaagatatat gagtcggtac gttggagaga
    19261 ctctcaccat taccacaaaa aaagcaagaa acgagaaaac acttggatta gcaatagaat
    19321 gtgatgaata tctgtctgta ttagcgcttg tcgttccgtt tcaactattc gcttataaaa
    19381 ccgcagtagc taaaggtatt gacttgaaca aaaagatttt tgaagacttt gatacagtct
    19441 tgaaaagtaa attataagag gtggaaatca tgctgaaatt caatgaagaa gaacaaatca
    19501 aagcaataaa aggcgcacta gctttacgtc cgcaagtaga agaaatcatc gataaacttt
    19561 atgcggaaaa atttgacgct gtctattatc taggaatcgg tggaacgtat gcttcctcga
    19621 tgcaagctgt cacttatatg aatgggaaaa gcaacttgcc tgtctttgta cagcatgctg
    19681 cagaatatta cactactgga aacaaaagat taacaaaaga ttctatcgtt attttgtcct
    19741 ctgttaccgg tacaactcaa gaagtagtga aagcagtaga acaaatcaag aaagtaggag
    19801 cgacactgat tggatttatc gataaagcga acagtaagtt gtctcagcta tgcgattttg
    19861 tggtcactta tccagctcct ggaacagaac aaatcaaatt ttttatggca gcagatcgat
    19921 tgatgtattt gaatggagaa ttcgaagctt attcagaata ctatgaacag ctcgaacgtt
    19981 atttaccaac agggttagtg gaagcagaga aaaaagcaga tgcatttggc ttatcatttg
    20041 cagaaaaaca tcgacgtgat agtatgcatt attttattgg agcagggaat caatggggag
    20101 ctgtttattc atatgccatg tgttactggg aagaacaaag ctggcttcca tcgaaatcta
    20161 tccatgctgc cgagtttctt cacgggacgt tagagatcgt agaagaaaca accccagtca
    20221 cattattttt aggcgaagat gaacagagag tgttatctga acgtgtggct aaacttttgc
    20281 caaaaatttg cagcaattat acattgatag acacaaaaga ctatccagta gaaggtatca
    20341 gtgaaaaata tcgcggacgt gttttgtctt tcttattgat gcatgtagtg acacaacgca
    20401 tcgatgctca tgtggagcag ttgaactgcc atccattaga gatacgccgc tactatcgtc
    20461 agtttgatta ttaggataga ctggataaaa agaatataaa aaataaagag agcagcgata
    20521 actgctccct ttattttttt aaaatattgg catcaattac gttataaaac acattttttg
    20581 cattatctac agttcaactt ataatctgtt taatgggaca tgtgggtgcc aaccattttt
    20641 tgttatgcaa tagtactatt tagttgttgc atggatccgt agtaaaagtc caagtacaac
    20701 gagttgcgcc agtttacata gcgttttgta tccagaatgt tgactaaaac aaaagttcct
    20761 atgtcaaccg tcaacagaga caatctgtcc gttgcaggac ctgacagatt tagaaatgtt
    20821 caatctgtct cttatgtatt ttaaatcaat actcactagg agaagtttcc taacctccta
    20881 atagtaatgc tggtagtaac attgtcaaaa ctgcttcctc ttaggaacaa gcaaagcata
    20941 acgagtgtaa aaataaaatg tagactattg taacatatca ttgattattt caagtaaaaa
    21001 cgataaaaga agtaaacaaa cacttaaaaa caatggaaat tagtcccaac tttaagaaat
    21061 gtatactata atggaaatta tcaattattt atttggagga atatccatga aacaaaaaat
    21121 cgcagttctt ggtcctggat cttggggcac tgctttagca caaacattgg cagaaaacgg
    21181 gcatgatgtc cgtatttggg gaaatgtacc tgaacagatt gatgaaatca ataagtatca
    21241 tacaaatcag cattttttac ctgatttgac gatcccagaa tcgattatcg gatataaaga
    21301 attggcagaa gcagtggata acgcagatgc tattttattt gttgttccaa caaaagcgat
    21361 tcgttcagtc gcacaggaac ttattggtaa aatgaatact aagccaatca ttatacatgc
    21421 aagtaaagga ctagaacaag atacgcataa acgaatctca gaagttttgg ctgaagaaat
    21481 tccagaagaa aaaagacagg caattgttgt attatctggt cctagccatg ctgaggaagt
    21541 ggcagtacat gatatcacaa caattacagc tgcaagtatt gatcaaaaag cagcagccta
    21601 tgtacaaaaa ctgttcatga atgattattt caggatttat acaaataatg atgtgattgg
    21661 cgtagaaact ggtgcagcac tgaaaaatat cattgcaatt ggtgccggcg ctatccatgg
    21721 attaggattc ggagatgatg caaaagcagc aatcatgact cgaggattgg cagaaatcag
    21781 ccgtcttggt gtcgcgatgg gtgccaatcc tctgacattt atcggtttga gtggtgtggg
    21841 ggacttgatc gttacttgta caagtgttca ttctcgcaat tggcgtgccg gtaacttgtt
    21901 aggacaggga cataagctgg aagaagttct agaaaacatg ggaatggtcg ttgagggtgt
    21961 ggcaacgaca aaagcagcag tagagctagc tcaacagcta ggtgttgaga tgccaattac
    22021 tcaaacaatc tataatgttt tgtataatgg agaagatatc aaaaaagcag caaaagaaat
    22081 catgttacgt gacggaaaaa tggagaatga attttctctt cgttaaatag atgatcaata
    22141 gttaatcgtc gtttaggagg aacagcaagt gaaggtcaaa aaagcagtca ttcctgccgc
    22201 tgggctagga acacgttttt tgccggcaac aaaagccatg gcaaaagaaa tgcttccaat
    22261 cgtggataaa ccaacgatcc agtttattgt ggaagaagca ttggattcgg gaatcgaaga
    22321 catcttgatc gtaacgggaa aagaaaaaag acccattgaa gatcactttg atgcaaatat
    22381 cgaattggaa aacaatttgc gggaaaagga aaaaacagaa ttactcgctt tagttgaaga
    22441 aacgactcaa gtcaatcttc acttcatccg tcaatctcat ccaagaggac tgggggatgc
    22501 cgtgcttcaa gcaaaagcat ttataggaaa tgaaccgttt gtcgtcatgc tgggggatga
    22561 tttgatgaaa gatgatatcc cattgactca acaattgatg atggattatg aagaaacaca
    22621 agcttccgct gttgccgtca tgcgtgtacc agaaaatgaa acatctaaat acggaattat
    22681 tgatcctaaa acagatagcg aaaagggtcg ttgcagagta aaagggtttg tagaaaaacc
    22741 tgaaatggga aaagcaccaa gtaatttagc cattatcgga cgttatctac tgactcctaa
    22801 aatatttgag atattagaaa ctcaagaacc aggagcagga aacgagatac agctaacgga
    22861 tgcacttcag actcttaacc agacagaggc ggtctatgcg agagagttta aaggaaaaag
    22921 atacgacgta ggcgataagt taggatatat gaagacaaat attgaatatg gacttcaaca
    22981 ttcggaaatt gggacttcat taagtgcata tattatccag ttgagtaaaa agctaagaac
    23041 agaaagttaa ctggaaataa gtggcctgct gagaatgaaa ggctatttgt tattaaagcg
    23101 gcagcttgtg cgtcatttgc gcaactgtcg ttttagtttt ttgactctag atcagttgag
    23161 tcgtggaaaa cgaaagatag aaaaagactt tcgtgaatag attcagaaaa tattatgcta
    23221 atggtttaat ttttttttaa gttgtataaa agaaaatgat tacataagag aaatgaaagc
    23281 caagccagaa gattcatagt agaataaaaa ggcgaatttt ttaattataa aaaaaggtgt
    23341 gtgttcacta tgggaaaatt agtcgctgtt gcacagtcgc tgcttgatat actcaagtgg
    23401 ctgatggaag gaagcaattg gatatttgtg ttgccactat tgcttattag tctatttatc
    23461 atgtttcgca tggtcagaaa aaaagaaacg tttctgattt ttatctggta tgcagcatcc
    23521 gttggggttg ctaccttgat ggttgaattg gcgttaggta gttgggaagc ttgcgtattt
    23581 gctttatttt ttgctgtact gtatctaatc atttatatcc catatatgaa aagaaagtct
    23641 cggataggca gaaaaggtag acatacaact actttttcta gtacagattc tctcgaacaa
    23701 agtagaagaa cgagaagaag acggtaatat ttagtaggta tacgcaaaaa aagataaaat
    23761 aatggataaa ctattgagat gagagattta tttaattggt ggaaaaaagc ttttttcact
    23821 tatataaaag aatctttttt aaaggaatgc ttgttgtatc gggataagcc attccttttt
    23881 ttatgttatt tatatatata taaatcatac caatgttttt ttgtattttc ttgcttgcac
    23941 ttggataaga gattacttac aataaattca gatagaaaca gctgaataaa aaaaggctcc
    24001 ctgaccctta gttggcgcta agaatcaggg aatgaccagt cacacacata caccctgacc
    24061 gatcattttg ccatagtcaa tgcagtgcat cgacctaatt tattgtacct aatctagggt
    24121 gaaaattcta cccctatgta gatattttcc atggtctgca cgttgtttta tttgctatgt
    24181 caaggtattg ggcgtattcg ttcaatgcct tttttgtgtt tgaaacagca aagaccacat
    24241 gtgttactgt ctaactactg tttttttatg tacttcctta gattaggcag ctgtttctat
    24301 tcatgtcaag tatagctagg cgggagagat aaatgagttt atgttatttt gactattatc
    24361 accaattgga agagaaatta aggatagaag agccgctgat gcttcaagct ggcacttgga
    24421 tcgcgaaaca aattaatcaa ggtggtagat tgcagatata tgctagccgc acactaagtg
    24481 gtgtagcatt tgaattttgg gatcaaaccc cggatcttgt accaagtata ttgattgaaa
    24541 atccagccgg aggtgtctat gaaacgctag aaggtgcagg acaggcaatt attgatcagc
    24601 tttctgtcaa atcagaagac atttttcttc ttctttcgaa tgaaggaaga aacccggcaa
    24661 tcatcgaact agctcagtgg atcaaagaaa atggtcatcc attaatcatt gttactggat
    24721 ttgatttgtc acgaagtatc aaatcgcaac atagcagtgg tttgcgatta tttgaatttg
    24781 ctgatttggt tttggacaac cacgccaatt tacatgatgc agtcttgact ttgccaaatc
    24841 tagatccagc gatctgcgga acagcctctc ttgcaacgat ctttttgctg cagcaagttt
    24901 tgtattttac agcaaaacag ctagtccagg acacaagtgg aaaatagtta tccacatgtt
    24961 gacaaagttc cttaaaaaaa taatgttcca cataaaaatg gagcgtttga aaaaaagaaa
    25021 acgattatta atgaggatta acaacgacta ctttttttgt tttagttatt ttaagatgga
    25081 tcatctttga ttttaagctt gttttgttta aagtttgtta tttttataag ttgtttcaca
    25141 gttatgagaa atgagttata agaatatgta gtgtcagaaa aataaaaagt agtggaaaaa
    25201 gagacaagaa agaatccata tggattcttt cttgtctctt aatacacgtt gatgaaagag
    25261 tcttccccaa aactctttac aaagtaatta tagtacattt gttatcatta ttgcaatgaa
    25321 gtttacctga tataacaaaa ttgtccaaca ggataccttt ttctaataga aataaaatac
    25381 acaaaaattg aaggatagat aatggatatt tataagtgtt tattattttt tttgcaatga
    25441 aattcaattt ttcaatgatt gtaaaatgaa aaaaacagga cgagattttc tctaatctaa
    25501 aaataaaaaa agaattttaa ggaaaaacag agaaataaaa tacttaatat aaatggaaag
    25561 agaaaaaaat aatcatacat aggagaaaaa aaacaaaata gaaaaaagtg attttctaat
    25621 aaattattca tgtggaaatg tcatttttac tgtataattc atttgattat ccatttttta
    25681 ttcatcaaaa ttactgaaaa aagaaaggaa gaaataaatt tgaagaaaac aaattcttca
    25741 ggaaaaccga aacgcgactt cactaaatta tcgacacccc atacgtatgt cattattttt
    25801 ggtgtagtca tctttgcatg gatactgact tttgtcgttc cagcaggaaa gttcagcacg
    25861 caagatattg aatacaaaga tgcaaatggc gaaacaagta ctcgtactgt tttacgtcaa
    25921 gattccttcc gttatgctta cgagctggat aagtcgtatg tgtttgatca attagaagaa
    25981 ctgcaagatc atccagcaga acgtgaaaaa ctggatgttc cggaaaaagg attggaaaaa
    26041 gtcattgctg acggagaaaa aaatcttacg caggaaaaac tagatgaaat ctccttgaca
    26101 gatgatgtgc tttacgatca gtacggcgaa aatatctatg atacttctaa aaaattgcat
    26161 aaaacagcca aaatatgggg aacagatgat tttggcggct ttggtttctt aaattttgtt
    26221 tttgaaggac tagtatctgg agataagtat ggatctgctg tagggatcgc tgcattgatc
    26281 cttgttgttg gaggtgcttt tggtattatt atgcgtaccg gagcgatcga cgcaggtatc
    26341 tatgcgttta tcagtaaaac taagggactg gagcgtttag ctttaccgct attattcttt
    26401 gctttttcat tcggtggcgc tacattcggg atggcagaag aggtaattcc attctcaatg
    26461 gtgatggttc catttgtcat tgcgttagga tatgactcga tcgtagcagt aaccgtgact
    26521 tatgtcgcat ctcaagtcgg aaatgcgacc tcttggatga gcccgtttag cgtagcggtg
    26581 gctcaaggga ttgctggaat ccccgtgttg tctggtgcga cattccgatt gattatgtgg
    26641 gtagttgtta ctgcattagc cgctggttat atgatgatat atgcagaaaa aatccgtaaa
    26701 aaaccagaaa gttcgttgac ttacaaatca gatgattatt tccgttctca tatcaaaaag
    26761 acatctgatg aaaataaaaa attcatgctt gggcataaat tgatcttatt agagatgtta
    26821 gttgtccttg tctggatcgt ttggggcgtg actcaaaaag gctattacat tccagaaatc
    26881 gcttctcaat tctttgtgat gggattagtt gctggaatca ttgctgttct tttcaaatta
    26941 gatggaatga aagtcaatga catcgcttct tctttccaat ctggtgcagc tgatttagct
    27001 ggtacagcaa tcgtagtagg tatggccaaa gggattcttc ttgtcttggg aggatctgat
    27061 gcatctgttc cttcagcttt gaatacgatt ttgcatggga tcgggacggc gttgaccggt
    27121 gttccagctg tcattggtgc ttgggcgatg tacatcttcc aaagtttgtt caacttggtt
    27181 gttacatcaa attcaggaca agcagctttg actatgccaa tcatggctcc actggcagat
    27241 ttagtaggtg tatccagaca agtagcagtt cttgcttatc agcttggttc aggttttatg
    27301 gacgctttta cacccgtatc agccagcttg attggtgtgc taggtgttgc acggatcgaa
    27361 tgggcaaaat gggcaagatt ccaaatcaag atgcaaggat tcctgtttgt tttaggaact
    27421 attttcatca tgattgccat tgcaataggt ctgcagtagt actggagacc tgtaaataaa
    27481 aaagtaatcc aaacagtaga taaggactcc aagaaccgtc attttacggt aaaatcaaag
    27541 gagtaaagat cctatcgttt ggattatttt ttaagatcct atcatcagtt atacttaaag
    27601 aaagaggaaa aatgtatgaa aatcattcga caaattgaag tgtttgcacc agattactta
    27661 ggaaaaatgg atgtactgat tgccggtggg aagattttgg ctgtagaaga acagctagaa
    27721 ggcggctacg aaggagtgga agtagaagaa ctttcaggtg agggaaaagt actaaccccg
    27781 ggtttcattg actgtcattt ccatatttta ggtggaggag gagaaggcgg ttatcaaaat
    27841 cgaacgccag aagtcacatt gagccaattg actacagctg gtgtaacgac agttgtcggt
    27901 tgtttgggaa cagacggaga aggtagagat atgactgcgt tgatcagcaa agcgaaaggt
    27961 ctggaagcag aaggaatctc cacttatgta tacgaaggtt cgtaccgatt gccggtaaaa
    28021 acagtaacag attctatcat caaagacttt ttgacaattg ataaaattat cggaatcgga
    28081 gaaatcgcag tatccgatca ccgttcttct cagccaagtt ttgaagagtt cgctcgtgca
    28141 gtagcagatg ctcgtgtagg aggcatgtta tccggaaaag ctgggatcat caatgtccac
    28201 ttaggcggag gaaaaagaaa gctagagcta ttgacacgaa tcgtagaaga aacagaaatt
    28261 ccgatcacac aatttttgcc tactcatgca aatcgcacac cagaattatt tgaagcttgc
    28321 gtggcatttg ccaaacgtgg tggcacgatt gacttcactg ctagcgaaga tcctgatttt
    28381 tgggagaaga cagatggtga agttcgcttc agcaaagcac tgaaacgatt gatcgaggaa
    28441 gatgtttctt tagataattt cacgatgacc tcagatggac aaggaagctt gccttatttt
    28501 gatgaaaaca atcatttcct agggttagga gtaggaagtg ccaaggcatt gctagtaggg
    28561 atcaaagaag ctgtgcaaaa agaaagtatc cctttggaaa tcgccttacg agctataacc
    28621 agtaatccag cacgtatatt gaaattggat aaaaaaggaa aaatcgaaat tggtgctgat
    28681 gcagatctgt gtatcttgga taaagaaacg cttgatattg atacagttat cgctaaagga
    28741 gaaataatgg tccaagaaaa agaagtcaaa gtatggggga ctttcgaaaa aag
//
LOCUS       DACECY010000035        28600 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.036, whole genome shotgun sequence.
ACCESSION   DACECY010000035 DACECY010000000
VERSION     DACECY010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.036"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            237..1877
                     /locus_tag="IWO38_001620"
     CDS             237..1877
                     /locus_tag="IWO38_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ0313712.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="IWO38_001621"
     CDS             2031..3353
                     /locus_tag="IWO38_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ0313713.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="IWO38_001622"
     CDS             complement(3415..3846)
                     /locus_tag="IWO38_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0313714.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="IWO38_001623"
     CDS             complement(3910..4746)
                     /locus_tag="IWO38_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ0313715.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="IWO38_001624"
     CDS             complement(4936..5238)
                     /locus_tag="IWO38_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ0313716.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="IWO38_001625"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="IWO38_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ0313717.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="IWO38_001626"
     CDS             complement(5692..5958)
                     /locus_tag="IWO38_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ0313718.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="IWO38_001627"
     CDS             complement(6212..6937)
                     /locus_tag="IWO38_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ0313719.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7303..7373)
                     /locus_tag="IWO38_001628"
     tRNA            complement(7303..7373)
                     /locus_tag="IWO38_001628"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7339..7341),aa:Gly,seq:ccc)
     gene            7490..8242
                     /locus_tag="IWO38_001629"
     CDS             7490..8242
                     /locus_tag="IWO38_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ0313720.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8258..8737
                     /locus_tag="IWO38_001630"
     CDS             8258..8737
                     /locus_tag="IWO38_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ0313721.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8988..10568)
                     /locus_tag="IWO38_001631"
     CDS             complement(8988..10568)
                     /locus_tag="IWO38_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ0313722.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10561..11304)
                     /locus_tag="IWO38_001632"
     CDS             complement(10561..11304)
                     /locus_tag="IWO38_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0313723.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KRLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11480..12256)
                     /locus_tag="IWO38_001633"
     CDS             complement(11480..12256)
                     /locus_tag="IWO38_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0313724.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12504..13340)
                     /locus_tag="IWO38_001634"
     CDS             complement(12504..13340)
                     /locus_tag="IWO38_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ0313725.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13351..14280)
                     /locus_tag="IWO38_001635"
     CDS             complement(13351..14280)
                     /locus_tag="IWO38_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ0313726.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14472..14825
                     /locus_tag="IWO38_001636"
     CDS             14472..14825
                     /locus_tag="IWO38_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0313727.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14899..15555)
                     /locus_tag="IWO38_001637"
     CDS             complement(14899..15555)
                     /locus_tag="IWO38_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ0313728.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15781..17715
                     /locus_tag="IWO38_001638"
     CDS             15781..17715
                     /locus_tag="IWO38_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0313729.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17801..19024)
                     /locus_tag="IWO38_001639"
     CDS             complement(17801..19024)
                     /locus_tag="IWO38_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ0313730.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19018..20916)
                     /locus_tag="IWO38_001640"
     CDS             complement(19018..20916)
                     /locus_tag="IWO38_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ0313731.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(20910..22028)
                     /locus_tag="IWO38_001641"
     CDS             complement(20910..22028)
                     /locus_tag="IWO38_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ0313732.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22111..22944)
                     /locus_tag="IWO38_001642"
     CDS             complement(22111..22944)
                     /locus_tag="IWO38_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0313733.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22937..23854)
                     /locus_tag="IWO38_001643"
     CDS             complement(22937..23854)
                     /locus_tag="IWO38_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0313734.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24081..25205)
                     /locus_tag="IWO38_001644"
     CDS             complement(24081..25205)
                     /locus_tag="IWO38_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0313735.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25388..26296
                     /locus_tag="IWO38_001645"
     CDS             25388..26296
                     /locus_tag="IWO38_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0313736.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            26437..28050
                     /locus_tag="IWO38_001646"
     CDS             26437..28050
                     /locus_tag="IWO38_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ0313737.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         8889 a         5621 c         4868 g         9222 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt
     7081 ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct
     7141 taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg
     7201 catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt
     7261 cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg
     7321 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa
     7381 aaacagtata cgcctctttc actccttcct caagtttttt tattctttac gcttccgata
     7441 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     7501 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     7561 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     7621 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     7681 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     7741 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     7801 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     7861 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     7921 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     7981 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     8041 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     8101 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     8161 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     8221 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     8281 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     8341 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     8401 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     8461 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     8521 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     8581 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     8641 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     8701 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     8761 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     8821 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     8881 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     8941 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     9001 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     9061 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     9121 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     9181 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     9241 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     9301 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     9361 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     9421 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     9481 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     9541 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     9601 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     9661 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     9721 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     9781 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     9841 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     9901 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     9961 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    10021 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    10081 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    10141 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    10201 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    10261 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    10321 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    10381 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    10441 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    10501 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    10561 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    10621 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    10681 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    10741 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    10801 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    10861 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    10921 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    10981 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    11041 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    11101 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    11161 aactaatctt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    11221 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    11281 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    11341 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    11401 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    11461 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    11521 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
    11581 tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat
    11641 ctttacataa atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga
    11701 gaatctcttc atctttcaat actaggccta ttttattttt ataacctggc atgctcatta
    11761 gataaaggat ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc
    11821 accttttttt cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt
    11881 ggatgctttt tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa
    11941 tctttcgatt aataacagtt attgctttat tcggatgaga cgttagataa acaattttac
    12001 acttttcatt cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca
    12061 aatcgatgtc taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa
    12121 caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc
    12181 tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa
    12241 atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat
    12301 acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac
    12361 acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa
    12421 atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag
    12481 ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca
    12541 gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa
    12601 tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac cgttactcct
    12661 tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg
    12721 aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca
    12781 tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct
    12841 aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca
    12901 ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat
    12961 tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg
    13021 gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc
    13081 aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga
    13141 tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact
    13201 aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg
    13261 atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat
    13321 aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct
    13381 aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat
    13441 cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt
    13501 gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat
    13561 cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag
    13621 cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa
    13681 ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg
    13741 gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg
    13801 gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata
    13861 ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc
    13921 catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat
    13981 cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg
    14041 atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc
    14101 agtaaaaata gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa
    14161 aaatacttgt aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat
    14221 aacgatcgat aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat
    14281 gagtttcctc ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa
    14341 aaaacaagtt gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt
    14401 gactttttct gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag
    14461 ggagaataaa aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt
    14521 catccgttgc cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc
    14581 cacgactaga ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg
    14641 aaacaccaga agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc
    14701 atcaaggaaa agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc
    14761 tactagatga actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa
    14821 aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg
    14881 ttcaatcgct ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg
    14941 caagaaatag atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc
    15001 tgacggagca ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt
    15061 taactgattg actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat
    15121 ttgttggatc ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac
    15181 gatgcgccca acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact
    15241 ttgatggaat ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac
    15301 atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc
    15361 ataaccatac ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc
    15421 actatctact tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt
    15481 atttaaaatc cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat
    15541 aatatgatct ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca
    15601 tcatagcagt atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt
    15661 ctttattcat ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac
    15721 tactaattga aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc
    15781 atgatattat tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa
    15841 aacatacatt tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct
    15901 ggaaaatcga ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc
    15961 gctaaaaata aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat
    16021 gaaacagttt ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg
    16081 atgcgcgagc tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct
    16141 tatttaaaag aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat
    16201 gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag
    16261 aaaatcgata ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg
    16321 caacaccctg acattttgat tctagacgaa ccaacgaacc atttagatat cgagacactt
    16381 tcctggcttg agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac
    16441 cgttattttt tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc
    16501 cattacaaag gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa
    16561 tggaaggctt atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg
    16621 aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa
    16681 atggaccggc tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata
    16741 gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat
    16801 gtcaccttat ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta
    16861 ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt
    16921 atccgtggaa aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa
    16981 gctgatctgc atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact
    17041 ccagaaaaag agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa
    17101 aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg
    17161 atgaacaaag aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac
    17221 aaagaagtat tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat
    17281 gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc
    17341 aaagtctatc taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc
    17401 gccgaattgt tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat
    17461 tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag
    17521 gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc
    17581 gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct
    17641 gctagacaag atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa
    17701 gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct
    17761 ttttttattt tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat
    17821 tactagtcat tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat
    17881 ttaccccatt atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt
    17941 ccgttgtagc atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata
    18001 agttcttttc tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca
    18061 tgatcaccat actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc
    18121 aataacaaat ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta
    18181 aatcaaaata tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc
    18241 atgcttggcc tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc
    18301 ttggttttcc atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta
    18361 ctaaataatc tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc
    18421 cagtttcttc actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc
    18481 taccttgttg tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc
    18541 aaaaacgctg aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc
    18601 ccgttatttt ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat
    18661 tagtgataat ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt
    18721 gttgaaataa aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc
    18781 cttctgtcca accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg
    18841 ggaattgata ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac
    18901 gctcgatctc attattccat tctttttcca ttaattcttt ataacattca tttatccatt
    18961 tttctggatg tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta
    19021 gcataatttt agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc
    19081 aaaatcaatt ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc
    19141 aaccactcct gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat
    19201 aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt
    19261 tcgtttaata gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct
    19321 atagttttcc ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc
    19381 aaaccctgtc tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg
    19441 ccagccttca aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt
    19501 agaggcaagt acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg
    19561 tccataggtt ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg
    19621 aatccctagc attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga
    19681 aaaaccaaat tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa
    19741 atttggccct aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag
    19801 caaattggct tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc
    19861 cgtccaatac tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa
    19921 tggagataac gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat
    19981 attcaggata cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt
    20041 gactaacccc ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga
    20101 ccaaaagctt gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc
    20161 atcttccgca aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat
    20221 ctggctaatg ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc
    20281 aaaaggatta taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag
    20341 ataacaattt tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc
    20401 aaatttttgg ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca
    20461 ttcatattca aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa
    20521 ttttttccat aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac
    20581 catacgctgg tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc
    20641 agcttttatt cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg
    20701 ccccgctccc cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc
    20761 taccccttga tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg
    20821 ttgatttatc tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg
    20881 atgtttcatt gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat
    20941 cgaagtaagc cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt
    21001 tctctatggt atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt
    21061 gcatagtgct tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat
    21121 cgtacagagg aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca
    21181 ctggtatcaa tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt
    21241 tcgattttct gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt
    21301 tttgtgtaac gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa
    21361 caagaattct cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca
    21421 tcaaattctg cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta
    21481 tctgcatgct gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa
    21541 tacagtaatg gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc
    21601 catgctacta aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt
    21661 aaccctagtt gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt
    21721 aagaataaaa aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat
    21781 aattgttctt gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc
    21841 tgccataaaa taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt
    21901 acgtctgtat attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat
    21961 tccgcatcta tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt
    22021 tcattcatgg atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac
    22081 gaactagatg gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc
    22141 cttaaattta gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa
    22201 aattagcata attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt
    22261 gataattaag ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata
    22321 ttgattccaa cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat
    22381 cggtacagcg attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc
    22441 catcagtgct gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact
    22501 ggcaatcgaa gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat
    22561 gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt
    22621 gaacaacagt ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga
    22681 actggctgta gctaaaactg tactgattac agcaataaca atcgaattcc aaataatcaa
    22741 gggataatta tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt
    22801 gaaattgatt ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata
    22861 taaatatggc atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct
    22921 gctttgtatc ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata
    22981 atataacaga tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca
    23041 tttcaaaact tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat
    23101 tggaagtccc aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta
    23161 aaactgtggc cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc
    23221 gaaatttcga tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa
    23281 gaccagctaa aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca
    23341 ttgatattcg tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat
    23401 tcaaaaatcc attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag
    23461 ccaccgttga tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta
    23521 actgtgctga attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg
    23581 atcctgccat gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga
    23641 tagctagata attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa
    23701 aagcaaacga gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg
    23761 tgaccaatcc ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct
    23821 ttattgtctt gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa
    23881 tcaccagtta attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa
    23941 ttttcaaaat cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca
    24001 tctgaataag tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt
    24061 tcatagtatt tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag
    24121 gccaatgagt tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct
    24181 gtcagcataa atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc tttagggaca
    24241 taaaaacttg atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc
    24301 agtggagcca tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca
    24361 taccagccgc cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca
    24421 ccgtagaaat gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc
    24481 gcttctttag tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg
    24541 gctagcatgt tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta
    24601 ccagtttttt cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct
    24661 tcaaaatcta cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta
    24721 aaatcactag cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat
    24781 ttttgatcat aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa
    24841 aaagcatctt gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata
    24901 tccggcatat cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc
    24961 gaagctacat tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt
    25021 gcagcatttt caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg
    25081 gcttttttta tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat
    25141 gcacttaagc tgaataatag ggtaatgttt agcaaagcta tcaccaattt gtatcttttt
    25201 ttcatgtcta tcccttcttt cttttcaagt tcttatataa aacgctttca cttatgtgta
    25261 ttaacggaat tagtaacaca aatatttata taaaaaaata ttttacttgt cgttttggct
    25321 tttcattttt gttataatag tcattaatta taaatagaaa gctttttata taaaaagaga
    25381 aaggaaagtg gttcttttgt taacaaacaa ccaatcctcc aatcttgtca aaaaaacact
    25441 aacctattcc atagataccc atcttcattt atctgctgct ccctattttc gactactaat
    25501 aaatgaaagc gctaatttat atgctgaatt tgcacataaa tctatagaac tagataatcc
    25561 ttccctttta ttcattgata aacagcaaga agtccaaatc ttttctactc aaaaggaaac
    25621 acaaactttt atcattatcc agttttattt tgatgagcaa ttattagcac atattacggg
    25681 acaatctttt tctatagatc aagaaataac atctatcatt acttttctga aaaatgaaca
    25741 gttcaaaatt gattatctaa aacatagtaa gccagctatt tcctatgatc aggaaatcaa
    25801 ttcttttttc atactatgcc aacagatgag cattcatttg ctttccttat tagttactta
    25861 ttgtctgcaa aaaattcaat tgtcttcctt accaaggcat tatcaacagg aacttgcttt
    25921 attgacaata ccttcaaatg agaaagtcat tcctaaaaaa aagcatattc tttattcaga
    25981 tgaattagtt gatccaatca tacgatattt agaagaacat ctttcagaaa attttttgct
    26041 tgaacaatta gcccaaattt tttttatcag tccagccagc ttaaaaaaac attttagaaa
    26101 agtgaatgga aaaagtgtta ttagctattt taaagaacta aaaatggaaa aagcaaaacg
    26161 tctcgtgcta gaaaatgaat taacctatac agaaattgca gatatattag gatttcattc
    26221 gatccatcat ttttcttcag cttttaaaaa atatacaggg ttatcccctt caaagtatta
    26281 tctatcctta aaatagaaaa aaatttttag gttttatcca caaattattt ctctatattt
    26341 tctgtctatt caaaataaac aataattttc cggcacaatt atttgctgtt ttttaccata
    26401 acatagtacg atcaacgtag aaaaggagat gtttttatga aagttgttgt cattggcggt
    26461 gtagctggcg gtccttcatt tgccactcgt ttccgtcgat tgaatgaagc gcacgaaatc
    26521 attatctatg aacgcggaga aaatatttct tatgcaagtt gtgctttgcc ttattattta
    26581 ggaggtgtga tcactgaccg tgactcgctg atcgaacgta caccagaaat attgaaaaca
    26641 aaaaacaaca tcgatgtctt tactaaacac gaagtaacag caatcgatcc ttctactaag
    26701 cgattaacag ttaaagacct atccacaaat gaagaaataa aaacagatta cgataagttg
    26761 atcatctctt ctggtgctag accagattat ccggatattc ccggagtttt tgaagcagaa
    26821 aacggctttg tcctccgtag tgtgacggat gcggatcgaa tcaaatcgtt ccttgaagaa
    26881 aaaaatccac aacatgtcgt cattcttggt gcaggtgtta tgggtctgga attagctgag
    26941 aatttcaagc atcgcggctt agacgtgact ttaatcgatc aattgccaca agtcgctttc
    27001 ccttatgatc cagaaattgc taatttagtt tatgacaaat tgctgaaaga aggattagcc
    27061 gttcatttag aaacaagagt tactgagatc cgtgataaag gtcgagaaat agtattatca
    27121 gatggctccg tcctttctgc tgatatgcta atttttgctg ttggtgtttc tccgaagaat
    27181 gaagtggtga aagcaaccgg catacaatta tcagatacag gtcagatcat tgttgatgac
    27241 cagttacaaa ccaatctgcc ggacatctat gcgattggcg atattatcga aacgactagt
    27301 gtagtgactg gtcagccgat ccaaagtatg ctttccagtg cggccaatcg tcaaggacat
    27361 atgctggcag atattttaaa tggtacgcct atgcgctatc gcggatatat tggtgcaggt
    27421 gtcgcaaaaa tctttgatca tacagcaagt tatgcgggaa tgacagaaca tgaattaaaa
    27481 gcatcaggca taacaaatta taaaactgtt tttatcactc cctttgacca tgcctatttc
    27541 tatccagaag ctacaagatt aaatctaaag ctgatttttg atgcagatag cggtcgtatt
    27601 ttaggtggac aagcatttgg agaaaaaggt gtcgataaac ggatgggaga actttctgta
    27661 gcaatcaccg gaaacttgac agtctttgat ttgcctgatt tggagttgcc ttactctcca
    27721 ccgtattcta ctacccgtga cccgttgaat atagctggtt atgtcgcaat caatcaaatg
    27781 acgaatatcg tagaaacgat caaagcaagt gatatacccg aaaacgattt gaaagaagcg
    27841 ttctttttag atatacgtga acctaataaa gcaccttctg gcagtatctc ggcaacaaaa
    27901 aatattccaa tgaatgagtt acgagatcga atcaatgaaa tcccaaaaga taaaaaagtt
    27961 tacattactt tcagaagagg attgaatact tatacttctg cccgaatctt ggcaggtttg
    28021 ggtatcaaag cgattttgat tgaagaataa aaaaacgacc gcggtgggta gccgcggtct
    28081 aggagttatt tttacttgag gagtaaaaat gaaaaagtat atagggttgt tggttggtat
    28141 gtatatagaa tagaagataa atgtgaagaa attgtgcatt atttcttcac atttattagg
    28201 ttatagttta agaaagttta aacattgcgc aatttaatta ttttagctgt ttcttttcat
    28261 aaaaaaacat gttgtgactc tgagacaaat catagtccat agtgatgtct ctagagtaac
    28321 tacgcctaat attacgaggc atcatattaa tctatcagca ctagtaccta aactagcatc
    28381 cctttcgaat attttttctt atataataaa gtttgctttc actcctctaa tctcaccaaa
    28441 tcgactggct tgcgttgtgt gatactagga actgattggt ttagaacgac ttttctattg
    28501 taatcatatt tttcgccatc ctcgttgtct tctaatcctt caatgaatga tccctaatgt
    28561 gtgtattaaa taatttaatt tttttgttcc tatttattta
//
LOCUS       DACECY010000036        27255 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.037, whole genome shotgun sequence.
ACCESSION   DACECY010000036 DACECY010000000
VERSION     DACECY010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.037"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            259..597
                     /locus_tag="IWO38_001647"
     CDS             259..597
                     /locus_tag="IWO38_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313738.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(802..1041)
                     /locus_tag="IWO38_001648"
     CDS             complement(802..1041)
                     /locus_tag="IWO38_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAQ0313739.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1017..1364)
                     /locus_tag="IWO38_001649"
     CDS             complement(1017..1364)
                     /locus_tag="IWO38_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ0313740.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(2096..2677)
                     /locus_tag="IWO38_001650"
     CDS             complement(2096..2677)
                     /locus_tag="IWO38_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0313741.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2860..3486)
                     /locus_tag="IWO38_001651"
     CDS             complement(2860..3486)
                     /locus_tag="IWO38_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0313742.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3508..4839)
                     /locus_tag="IWO38_001652"
     CDS             complement(3508..4839)
                     /locus_tag="IWO38_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ0313743.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4994..5395)
                     /locus_tag="IWO38_001653"
     CDS             complement(4994..5395)
                     /locus_tag="IWO38_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0313744.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5481..5687)
                     /locus_tag="IWO38_001654"
     CDS             complement(5481..5687)
                     /locus_tag="IWO38_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313745.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5782..7284)
                     /locus_tag="IWO38_001655"
     CDS             complement(5782..7284)
                     /locus_tag="IWO38_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ0313746.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7302..7757)
                     /gene="argR"
                     /locus_tag="IWO38_001656"
     CDS             complement(7302..7757)
                     /gene="argR"
                     /locus_tag="IWO38_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ0313747.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7855..8472)
                     /locus_tag="IWO38_001657"
     CDS             complement(7855..8472)
                     /locus_tag="IWO38_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313748.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8693..10066
                     /locus_tag="IWO38_001658"
     CDS             8693..10066
                     /locus_tag="IWO38_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0313749.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10141..11253
                     /locus_tag="IWO38_001659"
     CDS             10141..11253
                     /locus_tag="IWO38_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ0313750.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11325..12962)
                     /locus_tag="IWO38_001660"
     CDS             complement(11325..12962)
                     /locus_tag="IWO38_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ0313751.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13130..13738)
                     /locus_tag="IWO38_001661"
     CDS             complement(13130..13738)
                     /locus_tag="IWO38_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0313752.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(14122..15273)
                     /gene="queG"
                     /locus_tag="IWO38_001662"
     CDS             complement(14122..15273)
                     /gene="queG"
                     /locus_tag="IWO38_001662"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ0313753.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(15292..16044)
                     /locus_tag="IWO38_001663"
     CDS             complement(15292..16044)
                     /locus_tag="IWO38_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313754.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(16185..17135)
                     /locus_tag="IWO38_001664"
     CDS             complement(16185..17135)
                     /locus_tag="IWO38_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ0313755.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(17136..17975)
                     /locus_tag="IWO38_001665"
     CDS             complement(17136..17975)
                     /locus_tag="IWO38_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0313756.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(17972..18718)
                     /locus_tag="IWO38_001666"
     CDS             complement(17972..18718)
                     /locus_tag="IWO38_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0313757.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            18956..20326
                     /locus_tag="IWO38_001667"
     CDS             18956..20326
                     /locus_tag="IWO38_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0313758.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(20381..21277)
                     /locus_tag="IWO38_001668"
     CDS             complement(20381..21277)
                     /locus_tag="IWO38_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0313759.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21558..22235)
                     /locus_tag="IWO38_001669"
     CDS             complement(21558..22235)
                     /locus_tag="IWO38_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0313760.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(22326..23231)
                     /locus_tag="IWO38_001670"
     CDS             complement(22326..23231)
                     /locus_tag="IWO38_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0313761.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            23406..24830
                     /locus_tag="IWO38_001671"
     CDS             23406..24830
                     /locus_tag="IWO38_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0313762.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            24876..25907
                     /locus_tag="IWO38_001672"
     CDS             24876..25907
                     /locus_tag="IWO38_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0313763.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            25914..27101
                     /locus_tag="IWO38_001673"
     CDS             25914..27101
                     /locus_tag="IWO38_001673"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ0313764.1"
                     /translation="MEIKRSLNTKFAEIFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
BASE COUNT         7541 a         5599 c         4924 g         9191 t
ORIGIN      
        1 tatataacac taagtaacgc aaaattcccc tttgtaaact atctagaaaa ttgttttgtt
       61 taaagtatat cttattggat ataaatagaa ataaattgta gccagttcat tttttcttta
      121 actaaaaaaa taatccaaga aacattgaac ctatggatcc aaaagctacc caaatactat
      181 agctaacccc ccgctacctt caattttgca gataaaacgc tttgggagaa aatttcaaag
      241 acaacaggcc atcctatcat gcccgattat aaacaaaaag ctggctattc cttttgctct
      301 gacaaatttc aattgggaaa gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg
      361 atcaaagaat gcccgcttca aatagaaaca ttggttcaac gaatttcaac tagacaagac
      421 tttgctattg tagaacgtga gatcgtagct atttttgtag aagaagggat tttacatgat
      481 ccaactcata tcaatgtgga taaatggcaa cctcttatct ataaattcag agaatataca
      541 actgccaacc agtcgttaac tttgaatttt agattccaag aattcaaaga gtcataatga
      601 taaacatcgt taggtataaa taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc
      661 tatacattag aaaggtcgac tgtgacccta tagatgcagt atatgcagct tatgagcatt
      721 tcttctgttc tctttgtttt tgggatctgt gctgaagtct ttttcgtttt ttgccgctct
      781 ttctttgaaa aatcgattat gttatacctt ctccattact aattcaggca tctgctgagt
      841 aatgattttt cgcgcaaaag aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa
      901 gacaagcggc attcggtcat ggattggtgc gactgtgcgg ttaggttcag tagtcattat
      961 gattgattct gccatttttt catcctgatg gctacgataa aaaccgccaa tatacactat
     1021 tttttcctta tttgaaaaca taaatttgtc tttttcttca ttccactcat tgggaaaaca
     1081 caacgatttt gcaaaaatgg ttgatgaaag gtcttttttt cttctactgt ctctgcacgg
     1141 gcattaatca tcaattgtcc ttttttaaat ccggtaaatc cccacttcat gatttccgga
     1201 gtgaggatgt tttcttcctc ttttcttaac gtcacaacaa gatttgacgg aaaaacttct
     1261 ccagctgcta attctgctgc ctgcttcggt tgtttcgtct gggcttctcg ataataattc
     1321 tgaaggagct ctgactctag atcaaaaaaa taacgaccac acatcctaat ccctcccaat
     1381 ctttttttct atcatacact gcaacaagaa gttcgcacca acaaaacgct ttcaattttt
     1441 gtagaactaa aaaagttgtg acaagtgaca aactctccag tgaatggtat tctcctgcca
     1501 actcctcggt cacaagtcac aatattcaac aaacatgaga agctgctttt tgaaattgtt
     1561 ccttgtgact cggagtgaaa cggcaggctt acgacctctg ggtaaacggt gttcaaaagc
     1621 cagtttctcg gtattaagcc aaatttatgc aaaaatgtag aaagctattt tcacaaaatt
     1681 tttcttaata ctcgaaacta agcgactttt tcacaacctc ttccttaaac ggtgttcaaa
     1741 agcttgcatc tgcggtaata agcccaacca aacgaaaaat atgagcagct atttttattt
     1801 ggctgttctt attacttgat gcagggcagc tttttcacaa cctctccact aaacggtgtt
     1861 caatcagcta accttcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttgcc
     1921 gtttgtttct ctaatactca ggtcaaagcg ctgatttcac aacctctaat acacggtgtt
     1981 ccagaagtag cttcttcgaa aaataagccg aaatttcaca aaaatttgag aagcaatttt
     2041 cgtaaattcc ttcttatttc ttgaagctga ccacttctgt cacaacctct aattattaaa
     2101 gaaccttttg cgaagttaac aatctttctt tgatcaattg aaaattctgc ctaagtagtg
     2161 atagatcctg tgtgactttc aacaataaga tacttccctg gatttcagca atcaacgctt
     2221 tggcttgacg gtctgcttct tcatcggaca gtttttcttc agaaagtgcc aggatatttt
     2281 gtttcgtata cttgacccat tgattgacta attctagtag caacagacgg tattcttcat
     2341 ccactgtact caactcaata atcaaattac ctacaggaca gccatgataa gtgatctgtg
     2401 tctcatgatc agcgaaaatc cagtccaaca tttcttctaa tgcttcttct ggctgttttc
     2461 cagcatttaa gatgtcttcg aacaattctg tctgccatac gtccatatgc gcatggatca
     2521 ccgctagaca aacttctttt tttgaagaaa agtaataata aaattgacct tttcctgtat
     2581 cagaagctgc tagaatatct cggattgacg tttcatgata gcctttttta taaataagat
     2641 ctttggtcgc ttccaagatc ttttcctttg ttttcaatga tattctcctt tttcatttct
     2701 catagtccta tattatacag taaattcatc attttttctt cttaatcgtt taaaaaagac
     2761 tgtgccaaat tatctgacac agccctcact ataaaactgt tctcatatag ctagatgttc
     2821 attgattcaa gaactgcgag gacaaaagca attcctatct tatgatagtt gctctaatac
     2881 ttcctctgtt gtcatcactt gatctgcaat aaagccaaag ttcgtcaacg ctgcatccat
     2941 tgcttcttgg ccaggagcac caattgcatc tgtcgctacc acaacattga atccactctc
     3001 taccaattca cgcatatggg aatcgctaca caagtttccg ttcattccac caagaattac
     3061 tgtgtcgatt ccattttttc tcaattggag cgccaaatca ttgctttctg gtccaaagat
     3121 tttatgagga gagctgatga tcgtattttc atccaaataa ggtttcaatt cttcgataaa
     3181 atcggctcct gaaccttctt ccactgattc atattgactt tttctagcaa acatctgctg
     3241 ctcaagcatc atatgttctc ctgcaccgcc aaattgccat ttatggtcat gtgggtaaaa
     3301 ataatgagga gaaataaaga gtttgtatcc tttttctttt gcttgtttta ccatttcaat
     3361 catattttcg atcgtattgt atttttctaa gatatctttt gtcaaaccaa agccaccgcc
     3421 tgctggtttc aagaactcga cttgcgggtc agtgagaact actgctgtat ttttattatt
     3481 gaacatggtg gattctccat tcgttattta ttttaataca tcatttagag cttcactcat
     3541 tgtcggatga gaatagattt gatctctcaa taacgtataa ggaagttttc cattcatagc
     3601 tagcgaaatc aagttgatga cttcatgaga atcttccgca tagatacttg cccctagaat
     3661 caagtcagtt tcagggtcga ctaaaacttt gaatacacct ttaggatctt ctaatacttg
     3721 tgctttaggg acaccgcttg tcatatactt gaataatttg taattgacgc cttgtgcttt
     3781 ggcttgtttt tctgttaaac ctacattgga taaagtcgga gtgataaata cactgtaagg
     3841 cacagctttc cggttattcg ttgttcgttt attctcgccc ttcaactgat ccataatgat
     3901 tcgataatca tctagagaga tataagtgaa ctgcaatccg cctttgacgt ctccaatcgc
     3961 ccaaatgttg tctgcatctg tacgaaggta ttcatccaca gcgattgctc cgcgatctgt
     4021 tagtttaaca tctgtatttt ctaatccaag tgatgctgta cttggttttc ttccagttgc
     4081 agccaaaact tttgacgctt ggattgttac ttcttggcca tcttctgtaa aggtgacggc
     4141 agctgttgat tcttggtctg ctacttgatc tacagataca cctagatgga attcgattcc
     4201 cgcatttgtc atgtcttcta aaatcatttg cgcgatatct tcatcttcac gtggaagaaa
     4261 ttcagaatgg gcatcaagaa cgacaacttt agatccgtat tcattgaaca ttgatgcaaa
     4321 ttccaagcca atatatccag ccccgatgat cactaatttt tccggtaatt gttcctgatc
     4381 catcgcttgc gtagaatcaa tcaagtagcg acttgtattc aatcctttga ttggtaaaat
     4441 gattggttca gaaccagtat tgatgaaaat ccgttcgcct tcaacaatca ctttttcacc
     4501 attattcgtc acttcgattt gatggttaga aagaaaacgt gctgtaccgt ccatcactgt
     4561 cccagttgcc tcatcgctga tcatatgata atttttggcg cgaagcatgc ccgtcaattt
     4621 ttctttccga gcaacagcct ctgtaaagtc gattcctctt tctccattga aaatcaatga
     4681 tttagaagga atacagccga tgttgataca tgttccgcca tacatacgtg ttgacttttc
     4741 aacgactaaa accgattcac cttttgaagc taacgccttc gctaaagtct taccgccctt
     4801 tccaaaaccg atcacaatat tttgatattt ttccatcatt tttccctcgc ttctttttta
     4861 cctaccgatt ggtacaaaaa tatgataacc gataattgtc tattctccaa atcatctgct
     4921 ttatttgtca aaaaaaacca gacaaaaagc ggtcctttca cgtttttagt ctggttcgat
     4981 ttcctttatt cttctaaaca atcgttactt ctacatcgat atttcctcta gttgctttgg
     5041 agtaagggca gaattcatgc gcatcttctg caattttttg ggcttcttct tcgctgatgc
     5101 catcgatatg cacttctaat actacaccga tctgatagcc gccatcatct aaattataaa
     5161 gagagacacg tgcttttact gtggactctg ctgtcattcc tgcttctttg atcatatgtt
     5221 gtaccgcccc attgaaacaa gaagcataac ctgctgcaaa taattgttct ggatttgttc
     5281 cttctgcatg gattcctgga ggagtgattt tcatatgcat cgagccattt ggtgcctcga
     5341 cttctccatt gcgcccgcca tggttgatca ttgctgtttc gtatgctttt ttcattgtac
     5401 tgctcctttc gattgaccat ttgctacttg gctataatta tagtgtatga caagtaaggc
     5461 gctttctaca aaaattctgc ttattcgaat accagaatgc cgtcttctac tatttttcca
     5521 aaaaacaaaa aaatactact cgtgaacaac agcgtcatat ttgttccaaa ataaatagca
     5581 atcttttcta cataatcttt tatatgattg attttaagat acatatatac cagaaagttg
     5641 accccataaa ccaatactgt taaaaaaata accatattcg ttaccatttt ttcctccttt
     5701 tgctggtctc cctaccagca ctcgatttaa ccataccact ttttttgccg tccaagcaac
     5761 tagcctttca aaaagactgg tttattctgc tgttaatgat tcaagatcag aaaaatgtcc
     5821 gaataatttt gctaatttta ctaacgccaa cttgttcttt ccgaattcat cattttctat
     5881 ctgtaatact agtaacggct catgtaagct taaacggatc aaaaaccagc cgtttccgta
     5941 tggctcttct agatcatatc gaataccttc ttcattttcc gggtcgaagt tcatcccttt
     6001 ttcttcttcc aacagtcgct tcatttcttc taatatcttg tttccttgtt cgcgtggttc
     6061 atcgctgaac aattttaatc ggatttcttg tacttctacg ggctgtttca actctttgat
     6121 ttgttcatct aatgtgatac cttttctttt taattctggc aaaagcatca ggatctttgc
     6181 gatgacatat gcaccatcat ccagaaaata gttttccttg aatgcagcgt gtccgcttgt
     6241 ttcgatggct agctgtgtat ctttcccaag acggttaagt tctaatgctt tattgattac
     6301 atttcgatag ccggaaatat aacgaacttg gatgcctcct ttttcttcaa tgaatctttg
     6361 tagatgagaa gaagtagggg aattcgtcac gattcttgtt cctggatgct cttttaatac
     6421 gattgtactt aatactgcga tcaaattatt tcgattgatc actgttccag aagcactgac
     6481 aactgcagaa cgatccacat ctgtatcgaa aatgatccca agatctgctt catttgctaa
     6541 aacagcctgc ttgatacttt ccatcgcttc tttattatca ggatttggga tatgattagg
     6601 aaatcggcca tctggttcca aaaattggga acctgttgaa tccgcaccta agctattcaa
     6661 tactttttca gcaaagaatc caccagcacc atttccggca tcaacaatga ttttccaacc
     6721 atttagtggt ttgctttcta gtgttccaat ccccttttta atttttgtga ctaaatcttg
     6781 cgcataaggt gtcaaaaggt ctgctttttc tacgcttcca gtttgttcgc tttttatttt
     6841 ttgctgctgt gataggataa aagcaatatc ttctttttct gctcctcctt ctgaggtaaa
     6901 gaattttatt ccattataat aatagggaag atggctagca gtcagcataa ccgcagcatc
     6961 gcacgagaac tgttcaaatt gagtggacat aaacatagca ggcgtagtag ctaattcgaa
     7021 atcgatgaca tgtatccctt gttctttcag actttggatc aacgcctctt tcagtgctgg
     7081 tccgcttatc ctactgtcat gaccaatccc aatcgtccat tttttatttg gatcggttga
     7141 tttcttcttt ttctttatcc agtttgctag tccccagccg atctttttta ccatgtctgt
     7201 cgtcaagttt acttcatatc cttcaactgg taatgcgatt cctcgaatat ccgatccatt
     7261 ttgcagcgct ttcatatctt tcatataaaa gtcctcctca tttattccct cattttttcc
     7321 agcttttctg caaatcgctg tgcttcttct tctgagcggc aaataataat gaatgtatct
     7381 acaccggcca tcgttccgac aacttcaggg taagccactt catctagcca attcgttacc
     7441 acatcggcac catttcgttc agtcaatacg ataaccatga actgaacaga ctggatacgc
     7501 aaaacctcgc gtctaattgc tgtacgcaaa cgttcttcat ttaaagaaac agatggctgg
     7561 ttaaataatg tatagcgtac tattttgttt tcattttgac tttttactaa cttcatttct
     7621 cgaatgtctc gagaaatcgt tgcttgagtc acatcggcgc ctgcttcatg cagccgcgca
     7681 agcagctctt cctgtgtccc aataggttgt tctgtaatga tctggcggat cagtgcttgt
     7741 cgctgtttct ttttcatatt agacacctct caatgttgat tatagcaaaa gataagtaat
     7801 atttcttcat attttggtat tttttacttt tattttttgt tttcattcag gttgctaacc
     7861 tttttctatt ttaacacgca aaatcgtctt cattttttca ggtgctgatt ttcttggatc
     7921 gctgatgtaa atctctttgt gttctttaga agctctttca tagccttctt ttgcgatgaa
     7981 tgcttctaat ttctcaaacg ttgctggttc ttcggcatag ggcccgacgt gtaagatttg
     8041 ggcacaaaga ccttcttcca tctttttgaa tactacttgt tctttcaagt cttccggaag
     8101 tttgctagaa cgctgaatgg cttcttgtgc tatcgcttct gtaacaaagt caggctgtcg
     8161 gatcatcaat tgataagaaa aatgttcttt tttcatcact ggttcatgca aatacttttc
     8221 ttgcagtccc cattgtcctt ccagtggata gacagtatac atctgataat caggaatcag
     8281 ccaatttttt ttaggcgaca tgcgtattgc atagctgact gcatatagac aggctactct
     8341 tcgtttaaag tcttcctgat tcgggtcgcc gatccctctg atgctaaaga atgatttttt
     8401 aggaacctct agtatttgcg ggcttttttt catactatag ctcttgtctt tacgccattc
     8461 aattttttcc atttttcttc ctccgcgaaa ttccatttag ttatttatta tcatacctct
     8521 ttttgagtga cttggctaat cgtgttgaca ctatttttca gtttgtgcta gaataagtga
     8581 agtaaataaa tcgtacagat aacacgtttc ggcgtgtcga gagagaggcg gtttctattt
     8641 ttataataga aatcactctc tttttttacg aaatttttta gaggtgatgt aaatgacaaa
     8701 acaaaaacag ctgcttttag tacctgcagt aattgcaaca gggatgatgt cttttgcggg
     8761 cgtattgatc gagacggcca tgaatgtaac tttcccaact cttatcagac agtttgattt
     8821 aacgactgca caagtccaat gggtaacaac gatttatctt ttgatgattt cgatcattgt
     8881 tccgctgtct acttatttga atcgcaattt tagtctcaaa aaactctttt tagcttcaaa
     8941 tctgcttttc ttggcaggta taatgatcga tttcttctct cctaccttca tggtcctatt
     9001 gtttggtcga ttgctccaag gtgcagcgac aggtatcgct cttccgttga tgttccatat
     9061 tatcttgaca tttgcccccc tcaatcgacg aggcgcgatg atgggtgtcg gtactttgac
     9121 aacggccatt gctccagcaa ttggccccac ttatggaggc attatgactt cacattttac
     9181 atggaatcat atcttcctgt ttcttgtgcc cgttttgatt ctttctttga ttatcggctg
     9241 gtttgctatt cccgagatgg cagtacaaaa aagtggttat ttggattgga taagcgtttt
     9301 aggaatttgt cttttgttta gtggaagtct aacatttttg agtacgttgg gaacagtttc
     9361 cagctggatt gctttaatca tcggcggtat tggctggttc cttttttact atcgaagtaa
     9421 aaaagtggag catccactga tccgactgtc tattctttca aataaaacat tccgcttgtt
     9481 tttgattggc ttcttagttt atcagttttt attacttggt gtatcttttg tcttgccgaa
     9541 tttccttcag atcgttcaag gaagtagtcc ttttgtcgct ggattagcga tgcttcccgg
     9601 cgcagcggtt ggtgctttgc tttctccttt ttccgggaaa atgctggatc aatatggtcc
     9661 gaagaagcca atttttgctg ggctgattct ttctttgatt ggttggctag cattattcct
     9721 actcatagga cacgcttcag tgggtgtatt gattgcttgt catgtgtttt atatgatcgg
     9781 tatcggatta tcttacagca atatgatgac caccggaatg aatgcactga atgaagaact
     9841 acaaggagac gggaacgcaa tgttcaatac cttgcagcag ttttctggcg ctgtggctac
     9901 ttcattagta gcggtcatta tcaattacgt ccagcatcat acttcccata actatgaagt
     9961 gtctactaca ttaggatcaa aagcagcatt aggcgtcttg ttgctgttgc ttctcgtaag
    10021 ctttgctcga ttcgcctatt atctgttctt tgctaaaaaa gcgtagattc catatacggc
    10081 aggtgttgca tctgccgttt ttttgtgtga aaatggttgt ttgtaaaagg aggctttttt
    10141 atggaaaaaa ttgccattat cggtggcggg atcattggta tgaccctagc cagttactta
    10201 gatacatcaa aattcaatgt tgctttattt gatgaaggga caggacaggc aactaaagct
    10261 agcgctggga ttatctcccc ttggttgtcg aaacgcagaa acaaacaatg gtatcgtctg
    10321 gctaaagacg gcgctgcttt tttccctaaa cttataaaag atttccaact tgatcaaacg
    10381 atttacgcac aatcagggac acttttgctt cgacctgaaa aagatttagc tgaattggct
    10441 gttcttgccg agaaacgaaa agaagaagcg ccagaaattg gaaacatcga atggctttca
    10501 cctgaacaga catctcaagc acttccgtta ttgaaaccag cagtttcact gcgtatcagt
    10561 ggtggtggtc gtctggatgg aaaagcatat ttagaacatc ttggtcagcg tttagcacaa
    10621 aaacaaatgg agcagatcca ggcaaaagtc actttgcata agaagagcga ttcttggctc
    10681 atcaagggac cgtttggaga atacacagct gatcgtgtaa ttttgacacc tggcccggca
    10741 cttaagcctt tattacaaga attaggctat gctgcagata tacgtccgca aaaagggcag
    10801 ctcttagcat ttcagacatc ttataagatg agtcaagact ggcctgttgc tatgcttgac
    10861 ggagaagctg atttcattcc tttcatggat ggaaagatcc ttttaggtgc tacccatgaa
    10921 aacgatcaag gctgggattt ggctcctact agcgaagctt ataagggatt gactaagagt
    10981 acagaacagt tcttgactga tccagatcag ataaaaaact ggccatacga gttccgtgta
    11041 ggtacccgtg cttatacgtc agatttttcc ccatttttcg gcgtactgcc agatgatcca
    11101 agcttactgg ctgctagcgg tctaggctct tctggcttga ccacaggtcc ttatatcggc
    11161 tatctattag ccaacttctt aaatactggt cattggccta cggaagatta tcaaaaggac
    11221 atctccacct atattacgaa aaatccatca tagaaaaaca gctttttcaa gtaaggatcc
    11281 cgagcgatat tgcgcatgga ttacttcttg aaaaagctgt tattttagtt caatttctga
    11341 atcaaattct ccaaactttc ttttgcttca tctggcaaga gatcttttcc tatctgagct
    11401 tcaatgaatc gtaaacgaat atcaagtcgt tgtttcacca gatttttata ggacgtatcc
    11461 tcaaaagaaa caggaaaatc tggtataggt aaatagtgga tgtcttcaaa tggaccaaac
    11521 gacttgaatt ctgccgtttg ttgcacgatt ctttcaacga ttgacaatgt cgtttcaggt
    11581 ttgatttttt tatcatcgaa atatccagta ttcaggacat agcaggctgt ttgctgtttc
    11641 aaaaacaact ctcgaaaagc ttgatagtct tccctaagag gatagacacg gaaaggatta
    11701 gcaaatggtt caatgactag ctcatctagc tgtctttttg tagtgatatt ttccgcgtct
    11761 gatcgttttg ttgctaatgt tgcaccaaaa gacgctgcta aaactgggtc attgatcttg
    11821 ataattggcg gaagcgaatc atctttcatg atccaaaaaa cagcatcgat cttttcgcta
    11881 agatgatcga ctcggtcggg tgtcgcataa cgtgatttga ctgtccgccc atttccgttt
    11941 cgtatatctc ctgttactag gactttttct cctttacttg tacatgtcac gcccacattc
    12001 tgacaagtaa ggaaagaaga aatggccgga tcatcagacg gatagtcttg tgttttatca
    12061 aagtaagaag gttccaaggc agtagcaaaa ccattttttc gagaaataac gaaagcatcg
    12121 tcatgcaaga tcgaaacaga gtgaccttcc gtatgttttg ctaaggtaat cgttgatttt
    12181 ccagaaccgg acaacccaaa tacagccatc gtataggtct ggtcattact agcatattgt
    12241 ttcatcccac cgtggcaggc aataaagcca ttgcgatttc ccaatgccca agcaagcgtc
    12301 aaagtcgctt tttttaattc tccaaagtaa cgtaatccta gcacagccgc tgtattatgg
    12361 agagcatcaa acaaaatgag tccatttgga aatgtttcat gtgtccattc gggatcacaa
    12421 aagatataaa gatctccctc atcaaaggaa acagactttt gataagtctc taagatcttc
    12481 gaattcgctg gctgaaaatt cagcaaataa gaatacaaat tgttctcaaa tccttctgga
    12541 atcagcaaat gactttgcac agaaaattct tcagataggc caacgattac ttttgtatgg
    12601 taaaactggc gcttgcgact ttgatacaca gcttctctta aaattttctc gtatttgctt
    12661 tgttccttgt ctaagtcctc aaagatccgc cgagcacttg ctgttcttcc cacaatgctt
    12721 ccatcattga atactagtgt cctaacatca gctggtaaat ctagttcttc agcatgtgag
    12781 attgttaaat ctgtgatgat cgttcctgga ctcccttttg ctagttgata agctgtcttc
    12841 ctatcaaaaa cttcatgaac attattaccg taaaatgtcg tttctacaat cgatcgaata
    12901 ctggataaca gtggatttcc acttgtgatt tcgtttaatt cgagactctc ttttgtactc
    12961 atggctcctc ctcattccct aaacaaaaaa acgttttctt tattatacat tattcgagtt
    13021 tttatttgta ctgctgcgct gtgggaagca aacgagttat ttttcacaca aaaaaaccga
    13081 gaacttgaat aaacaagttt ctcggtttca tcattttcct tttatcggct tagtaccagc
    13141 cgtttgctaa ccagaatgtt ttagcagcat cccatgaacc ataacgagat gttacatatt
    13201 gttctgctac acgttcttga ttggcagctg aatagtcgcc gtttaagtat gaagcatcta
    13261 attgataacg gccgatgtaa cgtccgttag tagctgtgta tgaaccgcct gattcttttt
    13321 gtgcgatcca ttcttttgca gagcttgaag ttggcgctgt tgcagcagct ggggcagcag
    13381 gagctacttc aactgtttca acaggtgcaa cgtatgtatg ttcttgcgtt gctgtttcag
    13441 ttgtagatgc tgtattttct gttgttttgt tttctgttgt tgctgaagaa tcatttgaag
    13501 ttgggattgt taattgttca ccaacataaa tgcggttgat gtttgcgatc gaattttttt
    13561 cagcaattgc atcgatcatt gagttgtctc ctgcaaattt ttgtgagatc ttagataatg
    13621 aatcacctga ttttactgta taaacttcat cagcgtgagc atttgtagtt cctacaaaca
    13681 ttgcagtagc accagcagca aaagttgtac ctaaaacaat tttcttaaat gaattcatat
    13741 taaactcctt tttttctccg ttttttattc tttcgacgct attaacttta tcatttagaa
    13801 atatttcata ggtaaaagaa acatgacatt taatgacaaa ataacgaatt atttaatatt
    13861 tgtaacattt aaacaaatat ttttttgata caaaaccata aaagccttgt tgtataagca
    13921 aagtaatcgt ttttctatgt ttttttattt ttgaatgaaa aacgcagatt ctgtaacatt
    13981 cactgcaata atccataaaa aaaagcaaaa aaatttttga tttttatcaa attctcttaa
    14041 aaaaatgtaa aaaatacaaa aaaaccagta attgttacaa caattactgg acaaaaactt
    14101 tgaataatgt taactatttt tttattttaa ctttctccgc aataatttct gggcatgttc
    14161 caattcgttt ataacgactt cttctgtttc tttagctaat atttcatcta atacatcaag
    14221 ccatttttcc ggttcttttg ttcctagacg tccaatagcc caagctgctg ttcctctgat
    14281 gacagggcgg acatctgtta aacattctga aatctcaggc aatgccgttc gatcacctag
    14341 attagctaaa gcaatcaatg cgtttctctg caaaggtttc ttcccacgcc atgaaccagc
    14401 taaatgccca aattgctgtt tgaagtcttt attagacatc gaaagcattg gctttaattt
    14461 tggatatatt tcgtctactt ttggttccat ctcttcatga aaatgaaaat cttttccttg
    14521 attgtaaggg cagactaact ggcagatatc acatccataa atgacatttc gcattttttt
    14581 acgatattct tccggcatca tcccttttgt ctgagtttga taagacaaac atcgctgggc
    14641 attcattctt ccgtcaccaa gcaatgcttt tgtcggacaa gcagtcacgc agcgcataca
    14701 gtcaccgcaa ccaaattctc ctggtttatc aggctcaaat acaatattag taacgatctc
    14761 accaagatag acaaatgaac cgaactcttc agtgatcaac aatccgtttc gtccaataaa
    14821 tcctaaacca gctcgctgtg ctacagcgac atcgatcagt tctccagtat ctacttgcgg
    14881 agctagacgc cattcctctt ctttttgcca tttttctgct cttgtttgga tgaattcgat
    14941 caatcggttc aaacggtctc tgagtatatc atggtaatcg atcccccaag aagcacgagc
    15001 aaactgcccg cgtttttcat ccctaggcac ttcttcatgg atcctagtag gatacgcaag
    15061 tgcaatggaa atgatcgatt tcggctgatc aaatgtcatt tctggataaa gacgctcatc
    15121 gatatttgga tgttcaaagc cagaagtatg gccagctgct ttttgttctt ccaacgattc
    15181 ttttaatgaa tcaaaaggtt ccgccgtggt gaacccgatt ttgtcaattc ctaatcgttt
    15241 gctttcttgt atgatttctt cttttaatgt catggtcttc ctcgcttgct gttaacggct
    15301 gtataaccca taatatagtt tttctttttt cagctggaca aactggatct gctgatcggc
    15361 aatcttcttt tcttccatcc atttttttct tgcagcaaat acttcccaat aatttgttga
    15421 aattaatttt cttttgttat ctaatgcatt aaaaaaattc aatgaaactg gttttgtact
    15481 taacagttca tggagcctta cctcttccca tggcgaaaac tcagacagat cgcgggaata
    15541 caatacttct tcatagagat ttttttgctg tgtgtacgtg aatgtttgcg ggattttccc
    15601 aggcctagca atttctacta gacgattata aaacatattc attaatttgc tgttggcaat
    15661 aaattgactt ttataatagg ccgactggat ctttttgatt actccttctg ctttgatgac
    15721 tgaaaataat gcaggccatg ctttgacggt tatttcgtcg ttttgtttaa ctaatacgcc
    15781 aggtgtgaag ccgactggag aaaggaacac aaaataaccg ataccggtat tttcatctaa
    15841 aggctgaaat tcatcaaaca accatttgcc tatgtacagc tcttcatctg gttctgttgt
    15901 ctcattttga tttgctatga ataatttagc taattctgct tcaagaaatt gaaggtcttg
    15961 atccttgtcg gtattttcct gctggctgat caatcgaaag acttcttctt taatcgtata
    16021 tttatcttta cgtgtaaaat tcatagccac accttccttc cttactctaa ctataacgga
    16081 atttcttcaa aaaaggtatc tgtacgacga aaaacagcat aatttttttt catatctctt
    16141 ataaaaaatg caaggtctgc tcctcaagct agaccttgca cttttcagct ttcttttgtt
    16201 tgtgacaaac cttcatggat cgtttcaata ttccatttca tcatctgata gtaagaatct
    16261 ccttgttctc cttcttttgc tatggaatca gtgaacagtt tatcatagat tttcaaaccc
    16321 gtttcttttg ctacccgttc catgcttctt gagtctacac tggtttccac gaataaaacc
    16381 ggtacttctt ttgctcttat ctgatcaatg atcgctttca tttgatcagg cgtaccttga
    16441 ctttctgtat tgatctccca tatataagct gctggcaaat catatgctcg tgaaaaatac
    16501 ttgaatgccc cttcacttgt tactaataat tttttgttct cttcgataga agcaaaactt
    16561 tccttcgctt gtgtatctaa ttcttttagt ttttccacat atgcttctgc atttttttta
    16621 tagagtgtcg cattttctgc atctttttca gagaatatac gtgcgatttc ctccacatat
    16681 tttattccgt tagataggtc tagccaagca tggggatctg cttttgtatg ttcttcaccg
    16741 ctagttaaat aaagaggttc tacatttttg ctaactgcaa aataatcttt tccttcttct
    16801 tttttagccg tttccatcaa gttatcgaac cagctgttac ctgtttcaag attcaaaccg
    16861 ttgtataaaa taacatctgc atcgcttgcc tttttgatgt cttctggtag tacttcatat
    16921 tcatgcggat ctgttccgac tggtacgata ctgtggatat ctatttgatc tgttccgacc
    16981 tcttttgcca tgtccgccaa gatcgaattg gtagccacga ctgacaactt ttctttactt
    17041 ccttcatccg cttggtcaga agcctgcttt ccgcaaccgg ctaataccaa gaaactaact
    17101 gccaagatta aaaagagtga tttttttgtt ttcatctatc tgcttccttt ctgaaataaa
    17161 attccttttg ttggagaaaa taaaaaagca aggataaaaa atgcggtaca tgctaacacg
    17221 atcgtagctc ctgatggcca attgtaacta taactgaaaa acacaccgac aactgcactt
    17281 agcataccga ttcctgcagc tgtaatgatc attttcagta aatggttcgt tagcaagtaa
    17341 gccgtggcgg ctggtgtgat caacatggca atcaccaaga ttgttcctac tgtctgtaaa
    17401 ctgactacag ccactaatgt caaaaagaac atcaaaagat aatggatcag cgatgtgttc
    17461 agcccataag cttttgccat tgtcggatcg aatgatgtga tcttcaattc tttataaaag
    17521 aaaaagacaa atatcaagac gattccacta acgagtgctg tcagcaacaa atcgctttcc
    17581 ctaacagcta agacattccc aaataaaata tgatacaaat ctgtcgcact ctgggcatat
    17641 gaaatcaaga taatccctaa tgcaaaaaag gagctaaaga caattccaat cgctgtatca
    17701 tttttcaatc ggctccgctg agtcacgaaa ccaatcactg ctgctgttag catcccaaaa
    17761 gcagtggctc caaagatgta actaaaacca aacatataag aaatggctac acctggaaga
    17821 actgcgtgag agatagcgtc tcccatcaaa gacatccctc gtaaaataat aaaagaaccg
    17881 atgactccag aagcaagtcc tacaagcatg gaagtgacca aagcgtattg caggaattga
    17941 taattcatta atccatcaat aaaggattgg atcatttttt ctctcctcct tttactaaat
    18001 gtctgatgat ctcaccatag gccaattgaa ggttcttcga tgtaaagact tcttgtactg
    18061 gaccggaagc gatcagctgt ttatttaaaa gaataacctc atcgaaatat tcttctactt
    18121 tatgaagatc atgatggacg atcaaaatcg tttttcctga attcttcaat tcctgcaaga
    18181 tgtcaaagat ctttcgttca cttaacgcat caatccctac gaatggttca tctaaaaaga
    18241 tccattctgc tccttgggct agagctcttg caataaaaac tctctgaagc tgtccacccg
    18301 atagttcgct gatctgtctt ttggcatatt cttccatccc tacttttttc aaagcttgtc
    18361 ttgcacgttc tttttctttt ttcccaggtc tttgtccaat tcgtaaagat ggatatgttc
    18421 ctaaaagtac aacgcctaat acatcaattg gaaaggaaag atccaattca cttcgctgtt
    18481 ccacgtaggc tattttttta cggactgagt ttaccggctg tccagaaaag gtgactttac
    18541 cagtagtagc tggtatcaac tctagcaaag atttcataaa tgttgatttt cctgcaccgt
    18601 ttggtccgat aataccggta attttttgtg gatttatcgt caagttgata gttttcaaaa
    18661 cagttttccc ttgataagcg gctgaaaggt cttgaattac aatggccgta cgttccaaat
    18721 cttacactcc tttttaggtt tacctaactt tttatttaaa tatagtttaa ctaaaatgga
    18781 aagcattttc aagtagaatg atgaaaaagt ttttttcgaa ttttcctcgc attttatgaa
    18841 aattttctgt agaatcacat caagagttca ataaggctaa cctaataatg aaaaacatgt
    18901 gttgaaacgt ttgattattt tttcttgttg actcaacaac gaaaggagtt tttgtatgga
    18961 agaagaaaag ttaactcgaa gtcttagtgc ccgtcatatc caaatgatcg cactaggtgg
    19021 aacgatcggt gtcggtctgt ttatgggtgc ctcctcaacc atacgttgga cagggccctc
    19081 agtgatggga gcctatgcga ttgcaggatt ctttttgtat ttgattatgc gtgctttagg
    19141 cgaaatgctt tacgttgatc cttcgactgg ttcttttgca aattacgcga gcgagtacat
    19201 ccatcctgtg gctggctact tgaccgcttg gagtaatatt tttcaatata ttgtcgtagg
    19261 aataagtgaa gtgattgctg taggatctta tatgaattat tggtggcctg atttgccggc
    19321 aattattccc ggaatcattg tcgtcttgtt tcttatgctg gcaaatctta tttctgtcaa
    19381 ggcgtttggc gaattagaat tttggttttc gatcatcaaa gtcataacga tcattttgat
    19441 gatcattgca ggtcttgggg taatcttatt tggatttggt aatcatggtc aggcagtagg
    19501 gatttcaaat ctttggaaaa atggtggatt cttcactgga ggcgtcaaag gatttttctt
    19561 tgcattatcg atcgttgttg cttcctatca aggaatcgaa ctgatcggaa tgactgctgg
    19621 tgaagcagag aatcctaaaa agacgatcat tgaagctgtc cagtccacta tcggtcgaat
    19681 cttgattttt tatattggtg ctatttttat cattgtgagt atctatccgt ggaatcaatt
    19741 gagtgagatt ggttcacctt ttgtacaaac tttttctaaa gtcggcatta catttgctgc
    19801 aggactgatt aattttgtcg tgattacagc ggcattatca ggttgcaatt cagggatttt
    19861 cagcgcaagt cgtatggtct atacacttgc cataaatgga aagatgtcta aaaaatttct
    19921 gaaactatct cgtcacggtg cgccttttta tcctgtcgta gcaatctcag gtggaatctt
    19981 aattggtctg atcttaaatt atcttctacc aatgttcatc aaccagtcag aaaatatttt
    20041 cgttttcgta tacagctcaa gtattttgcc tggtatggtt ccttgggtag tgattttgat
    20101 cagcgagatt cgcttcaaaa agaagcacgc agataaaatg catgatcatc cttttaaaat
    20161 gccactatct ccttatacga attatgtgac acttgctttt ttagctatcg tattgatttt
    20221 catgtttatc aatcctgaaa cgagaatctc attgattatt ggactcgttt tcttgcttta
    20281 catgacgatc cattactttg tacgcgaaaa aagaaccgga aaataaaaaa agaacgaagc
    20341 ttcaccttag tggaagcttc gttctttttt attgctttta tcaattcaat tttagcgtct
    20401 caccgggagt caggaaataa ttctccatgc tcatcccatt cagcgacaag atcgtttcta
    20461 ctggtacgcc agttcgttcg gctatttgat ttggaccttc tccttccaaa actacggttg
    20521 tcgcagcctg tgaagtcgcg ctgttggtat tagtttcaga ggaatctgtt gtgtcttgtg
    20581 ttgttcgagc ttgcatttca gaagcttcta ccgactctgc tgtctcagaa tcagaagatg
    20641 ttgtgtcttg tgtgcttgtt gactctgctt gaagagaggc gctagtctgc aaataaatat
    20701 cggaaatctt agcgacatga ttcgtataat tagccgataa ttgattgatc aaagtatcta
    20761 agtctacacc gtctttattt tctgctgcct gatacgctgc acgtaatttt ctgatccctt
    20821 cgtcggagat tgcttgaagc tcacgtgctt tttggttagc aactgctgat aaaccagcta
    20881 ttccctcttg atttccctga atttctgtct ggtattcttc aatcaatgca ggcgtctttt
    20941 cttcgatctt tttcgaataa tcatttacga ttgcttgaag ttgctctggc aaaccatttg
    21001 ctgttttttc ttcagaagtt tgtgtctctt ttgttttagt tgatgactca cttgtttgtt
    21061 tcgtctctgt tttctttgtc gttgtttccg ttatggtcgt acttttcgct tctgacgaat
    21121 tttttccatg aaaatataga cccgcaactc ctcctgctat gatcaaaaac acggcaactg
    21181 ctgccccaat catgatcatc cgcttacgag ctgcctgttt ttgctgtctt cttcgggtca
    21241 tccgaacatt ttctccccac tcattttcat catacatttt tttcaccacc aatttttatg
    21301 tataatagcg ttatcaaacg cttcatattc ttattgtagc aaataattgt cgctgtgcac
    21361 atttttatct ttgttattta taagaaaatt tcaccagttg agatgattcc aagattgtcc
    21421 gtcgatttct ccttgcagct gcattgttcg ttcgtctatc tcagccatca gtttctttat
    21481 ttcatataag agcgcacggt cttgatattc tgatttttcg cttaattcat ctaattgttg
    21541 gatcagccaa tcatttgtca taatgaagtt cctttcattt cttgcagtgt aaaggtcaag
    21601 tcttcgatct gctcttttgc ctcagtcagt gcttctacga tatatgcttg tttttcagat
    21661 tcttcttcct gcattttctg attttcctgt actctttgaa taaaccagtc aatccgctga
    21721 gtcagcgatg gtttgccttc caatgagaga tactgctgat aagcaattag cttctcccaa
    21781 cgtttttcct gggataaata cataaattgt gctaaaacga atggcggaag ataggtcata
    21841 tggaatttat ttgcgattcg ctgatacggc ctcaaaaatt cggaaatcgt atattcttct
    21901 tttcctccag cttggtattc tttttccgcc acacctgttg tcaatacgat tcctagttct
    21961 ttccctttta atacatgtgc tgctcgttcc aagacattat cttgccaaat ttttaaatga
    22021 gcaggagaac tataccagta aaacggaaac tggaaaatga tccgatcatg ctgggccaag
    22081 agctgtagtt cagtctctat atctattttt ccgtctggat aacttgattc caaatgatgc
    22141 caagtagctg gtaaaccagc agaagcttct ttgaaaaatt gctgatttaa agattcttcg
    22201 atcgttggat ggctaatgat gataagtgta cgcatccctt ttcctccttt ttagtcttcg
    22261 ctttacagtg tagcataacc gccacaagtt tcttttgccg atgcttgtca ttttaaccag
    22321 actaatcagg ttgtccaaga aactgcggtt tgtttttcca actgtttttt caaggtatcc
    22381 aaagcaatat tcttaaaatt ttcgtttttc atacgtttac gatagttcgt agccgtttct
    22441 ccggtactct ttttgaacat tcggctaaat acgactagac tattgaaccc tacttgatag
    22501 gcaatacttt ccacggattc atccgtcaga gaaagaagaa acgcccctcg attgatccgt
    22561 accccgatca aatattcctt gatactggtg cccatatgat ttttgaatac tcgcgacaaa
    22621 tggctccgat tgaccgctaa atgctgggcg atttcctcaa cagacaattt ttcagcataa
    22681 tgtgcctcga tgtatttgac tgtttcaaca gctaaagagg aatgaacctt ttgtacacct
    22741 ccgctgacct ttccgccatc ttttaaaagg actgataaaa aacggtaggt taatgctgtt
    22801 aactgtaatt cattttccaa ggtttcttcc gtatctttca tgcattctaa aataatgtcg
    22861 atatattttt gtatgtcaga agagaccatc gtgtattggt cttctctgaa aagcgactgc
    22921 cgaatgattt cttctgcagc actcccacca aatgaaagcc agcaatacat ccacggatct
    22981 tctccatctg ataagtaaaa cgtcgagtct cctggacgaa tcaagaacag atcacctttt
    23041 ttcaattgga attgttgatt tttcacatga taagatcctt tgccttccat cacgacatgc
    23101 aggatgtaat cattgcgaat agcaggacca aaagaatgaa acggcatcgt tttagagaaa
    23161 ccacaaaaat caaaatacaa ttctttttct acttgttgat gttccttttt taaaaaaatc
    23221 gattgctcca cgtctatccc tccttactcc tttagtataa tgcaacaaaa agttaaataa
    23281 aagtcacaga aatatattaa aaaggaagcg atttcttgtt aagtttaagt cattaaaaga
    23341 tctttgttat tcgttgtaac cggtttactt agtattatat attgaacagg agaggaatac
    23401 agattatgaa aagcaaaatg atccaacgtg tgtcctacgc tttaggcgct tttggacacg
    23461 acacattcta tgttacatta agcacttatt ttatggtatt cgtgacaagc cagatgttcg
    23521 caggaacaga taaggcaacg aatgcaaaaa tgattgctac agttacaagc ttagttgtag
    23581 gtatccgctt agttgaaatt atttttgatc ctttgattgg tggcatcatc gataatactc
    23641 gtacaaaata cggtaaattc aaaccatggt tagttatcgg cggcgttgtt agttccgtac
    23701 tcttggtagc aatctttacc aatttctttg gcttagctac tcataatacg actttattta
    23761 tcatattatt tgcaatcgtt ttcatcttat tggatagttt ttattcattt aaggatatcg
    23821 ctttttggtc aatgattcca gctttgagta cggaaacaaa ggaacgtgaa aaatttgcta
    23881 cgtttgctcg tttcggctct tctctaggtg caaatggtac tactctagtc gttgtcccta
    23941 tcgttacttt ctttacctat cttgttactc ataaacatat tgaaggcgct tcaggctggt
    24001 tctggttcgg tgtgattgct gctgttattt caggcggtac tgcgattatc actgccttgg
    24061 gcacaaaaga agtcgatact gcgatccgtc agcaaaatga aaaagctggg atcaaagatg
    24121 tgttcaaagc agttgctcaa aatgatcagt tgatgtggct tgcccttgct tatatcgctt
    24181 atgcgattgc caatgcagca acaactgccg tattattcta cttcttcaaa tatgtacttg
    24241 gccaaccaac tgaattttgg atcgttggtc tagttgctgc cttacttggt ttagtagctg
    24301 ttccactatt cccaatgctt acacgcgtca tctctcgtcg ctacgtttac cttggaggga
    24361 tcgcaatgat gatttcttct tactttttct ttacgatcgc aggttcaagt ttgccaatcg
    24421 ttatcatcgg gctagtattg ttctacttcc ctcaacaatt gattttctta tccgctttga
    24481 tgacgatcac agactctgtt gaatatggtc aatggaaaaa tggtatccgt aacgaagctg
    24541 ttactctatc aattcgccct ttactagata aaatcgctgg tgctttgtct aacgggatcg
    24601 tagggtttgt cgctatcgct tctgggatga ctggtaatgc tactgctgct gatatcactg
    24661 ctcatggcgt aacgactttc aaagcttatg cattctatgc tcctgctgct ttgatgattt
    24721 tctccgctat tatttttgct tggaagatta cattgacaga gaaaaaacat gcacaaatcg
    24781 ttgagaaatt ggaaaacaaa ttggctccag ctgatacaat ggaagattaa cttcacgatg
    24841 cagtagttcc ttcccattaa gaaaggatct tgaaaatgaa gattacagaa aaagattttg
    24901 gccaaggtta tcacttgata accttggaga acaaaaacaa gctagcactt tctatctctg
    24961 atttaggtgc tagaatcgtc agtttaaaat caaatgatcg agaattagtt ttgggatttg
    25021 acaccgctga agaatatatc gaaaaagatc cgtatatcgg tgcttctatc ggtcgcaccg
    25081 cagggagaat cgaaaatggc cgcttttctc tcaatggaaa aacttaccaa ttagccacag
    25141 atccaaaaac tggacataat ctgcatggtg gtgctcctgg atttgaatta aaaaaatggt
    25201 cttatgtcat tttgaatggc gaaaatgaag cttctgtcat ttttactact acaagtcccg
    25261 acggagaaca tggttttcca ggtacaatgg atgtggaaat ccgctatact ttgacaaaag
    25321 ataatatctg gcgagtgacc tctcgcggca ccagtgacca agatacttta ttcaatccga
    25381 caaatcatgt ttacttcaac ttgacgggag atgcttctca gtcaatcgat caacatgagc
    25441 tttggctcaa tagcgaagct tatgctcctt tgcgaacaga tagtattcct ataggggtaa
    25501 aagaaaatgc tgcaggaagt gctttcgatt ttcaaatacc taaaaaatta gcgtccgttt
    25561 ttgctagtga tttggatcaa aaaaatcttg ttgacggaat cgatcatcca tttttcttaa
    25621 aagaaaccgg cttgggtaaa gaagccgctc gcttgactag ccccgataaa agaatccaag
    25681 tagatattgc tacagatgct tctagtgtgg ttattttcac tgcaaatttc ggcacagaaa
    25741 caccagaaat gcgcaaccga aaactggcac atcacggtgg catcacattt gagactcaaa
    25801 cagctccagg tgcagagcgc ttctcagcat ttggttcgat tcatttaaaa gccggatcag
    25861 tatttgaaac agtgacagaa ttcaaaatca aaacaagaaa ggaataactc atcatggaaa
    25921 tcaaaagaag cttaaacaca aaatttgccg aaatattcgg acctaaaaaa actacacaat
    25981 acttctcacc aggacgcatc aatttgattg gtgaacacac agattacaat ggaggccatg
    26041 tcttccccgc ttccatcact tatggaacgc aaggtgtagc agcacctcga gacgataata
    26101 aaatattagt ttattccaca aactttgacg atatcggtgt cattagtttt actttagatg
    26161 agctagaata tgataaaaaa gcaaactggg caaattacgt aaaaggaatg gtccttaaat
    26221 taaaagaagc tggacactca atcgatcatg ggtttgagtt attagtggaa ggaacaatcc
    26281 ctaatggtgc aggactttcc agtagcgctt cattagaatt attagtaggt gttgtattag
    26341 aagatctgtt tgatttaaaa acagatcgct tggaactagt ccaaacaggt aaaaaagtag
    26401 aaaatgaatt catcggtgtc aattcaggta tcatggatca gtttgccatc ggcttcggtg
    26461 agctagatca tgcaatctta ttagatacaa atacgctgaa atacgaaatg gttccagtga
    26521 aactagatgg ttatgcaatc gtcatcatga atacgaataa gcggcgggaa ttggcagatt
    26581 caaaatacaa tgaacgccgt agcgaatgcg aagaagcctt ggcacgtttg caaaaaactt
    26641 tgcctatctc ttctttagga gacttagatg aagaagaatt tgaatcaact attgaccaaa
    26701 tcggcgatga tacattgata cgcagagctc gccatgccgt ttctgaaaat cagcggactt
    26761 tgaaggcaaa agctgaatta gaagctggga acttagaagc atttggtcaa ctgttaaatg
    26821 attctcacca ctctcttcgc tatgattatg aagtaactgg gatcgaatta gatacactag
    26881 tagatgctgc tcaaaaacaa gaaggtgtct taggtgcacg aatgacagga gctggatttg
    26941 gtggctgtgc gattgctctt gtcaaagaag aaaatatacc tgccttcaaa aacaatgtct
    27001 atgatgaata catgaaagtt gtcggctatg ctccggaatt ttatgttgca cacattggaa
    27061 atgggacaac aaagatcgat ccagaagctg ttcatgtttg ataaaaaaat caacgatctc
    27121 agctaaaaac aaatagataa aaataacctc tcagcaattt ccaaatgtga gccttttgct
    27181 cgtatttgaa aattgctgtt ttttattgac acggaaagag aacgtgtgtt ctaattgtgt
    27241 tgaagaggtg attgt
//
LOCUS       DACECY010000037        27011 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.038, whole genome shotgun sequence.
ACCESSION   DACECY010000037 DACECY010000000
VERSION     DACECY010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.038"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1643)
                     /locus_tag="IWO38_001674"
     CDS             complement(<1..1643)
                     /locus_tag="IWO38_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ0313765.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDEC"
     gene            complement(1806..2399)
                     /locus_tag="IWO38_001675"
     CDS             complement(1806..2399)
                     /locus_tag="IWO38_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313766.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(2400..2732)
                     /locus_tag="IWO38_001676"
     CDS             complement(2400..2732)
                     /locus_tag="IWO38_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313767.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(2782..5313)
                     /locus_tag="IWO38_001677"
     CDS             complement(2782..5313)
                     /locus_tag="IWO38_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313768.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNNVESLPTDKRNALTAKNFKTANINSVII
                     PKEAAKMAGNDKSGLSDIEKDRLKALQYRLELDDNGKEIPVEISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            complement(5310..7922)
                     /locus_tag="IWO38_001678"
     CDS             complement(5310..7922)
                     /locus_tag="IWO38_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ0313769.1"
                     /translation="MRIRETTKRKDKPFNLEKKKYLSHFYSYAPAGERLPGKHDKRVK
                     QKLMYSGLVYLPLDYLVTRIPEAFSRFEIIQGSLQGVNLFQLQEMPQVFHFDSAGFWN
                     PLTPATSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPDAKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKNELYVASKDELEARGYDVYAYNLTEPLQSMSDNPLALIVKYWQRGDV
                     DTATQLANTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPTNEFKQLNALDEFMNQLPPGNLAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEKGDQVCLKYDHQRKEYVAWYEFSQRELKDEEGNIV
                     YRKKRGETHLPEWEKEAVLTLDESKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHIV
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPAMDNMEGIMTVTAGRNMLW
                     DLFIQSYKQLRAKYGEDNADIIKENCQTHVYIMSTDDNTIEEFSKKVGNKTVEQENST
                     VNSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRRDLKGNKIRPYPIFNSE
                     KSNSPYAYQYLEDFDTLGRTIHDIDIYCTHADLDLKALQIDYDYFLPDEDARDLYHGL
                     HEQLGEKIEENMFYKIVQSLENETAQKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     GKRLLLESEKVVKE"
     gene            complement(7922..9976)
                     /locus_tag="IWO38_001679"
     CDS             complement(7922..9976)
                     /locus_tag="IWO38_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313770.1"
                     /translation="MYFTIAGNTKTDKEVIKKATKEVGKRTSFLPKTIKYSVIEMEEQ
                     LHVLGKVLEQESQSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELETDQRKVNQVSEVEANEEVPWERPIFPK
                     DESKEKEKGIADEQADISTHKSIFEEENEVMDQLEVDPFEETMEDEETAEVEIDPVEA
                     NEEPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYKLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNGQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYETQQQELKEQEEKQRQEEKEREQLEIKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAIQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKASDSSKTKEGSSDSTVQSNSSESSESNE
                     VK"
     gene            complement(10046..10348)
                     /locus_tag="IWO38_001680"
     CDS             complement(10046..10348)
                     /locus_tag="IWO38_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313771.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAGVAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILGAASIITSLFGLFGQTPQGL"
     gene            complement(10366..10608)
                     /locus_tag="IWO38_001681"
     CDS             complement(10366..10608)
                     /locus_tag="IWO38_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313772.1"
                     /translation="MIAGLFIYPKGGIIVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            complement(10605..11069)
                     /locus_tag="IWO38_001682"
     CDS             complement(10605..11069)
                     /locus_tag="IWO38_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313773.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     RELGGNWYKLKIIDDTTWEYSEEMNPEPVQVSVEKQKDYQGLERYKVVDSAGVSEFIN
                     KPDYLFIVVPYEKDGVQKMILLGSSKDEKRTKEKLIHDGSELNKYKLQKTSN"
     gene            11257..11448
                     /locus_tag="IWO38_001683"
     CDS             11257..11448
                     /locus_tag="IWO38_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313774.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            11592..13658
                     /locus_tag="IWO38_001684"
     CDS             11592..13658
                     /locus_tag="IWO38_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAQ0313775.1"
                     /translation="MKLLNPKTVIAGTFAVTLAATLALAGSVDAASTSVMYRLYNPNN
                     HEHFYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEV
                     KWLTKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWK
                     YEGIGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDS
                     KATQKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQ
                     TALSNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLL
                     KEEHVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVE
                     VKARPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPTFTNFVLDPRESSSNTVTLN
                     NVTSNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFL
                     CRNDDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQN
                     GVAPMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYL
                     YVDEILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFIS
                     ANPIFK"
     gene            complement(13694..15784)
                     /locus_tag="IWO38_001685"
     CDS             complement(13694..15784)
                     /locus_tag="IWO38_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0313776.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVSGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKDGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVDYVDAHVECGSNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQDG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSTGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVAAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFSYAVLKFN"
     gene            complement(15781..16041)
                     /locus_tag="IWO38_001686"
     CDS             complement(15781..16041)
                     /locus_tag="IWO38_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313777.1"
                     /translation="MKLIKLLLFNLSCLTAVFLYCGLLFSQLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            complement(16054..16815)
                     /locus_tag="IWO38_001687"
     CDS             complement(16054..16815)
                     /locus_tag="IWO38_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313778.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTVADSQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLSTDQIESQLKDRVKKLSSDQLKLVSNGETKTIDQQA
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            complement(16831..17583)
                     /locus_tag="IWO38_001688"
     CDS             complement(16831..17583)
                     /locus_tag="IWO38_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0313779.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAAGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAASTDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVSFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            complement(17598..19574)
                     /locus_tag="IWO38_001689"
     CDS             complement(17598..19574)
                     /locus_tag="IWO38_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0313780.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQAHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            complement(19627..20301)
                     /locus_tag="IWO38_001690"
     CDS             complement(19627..20301)
                     /locus_tag="IWO38_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ0313781.1"
                     /translation="MDEKMVIFFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKIPV
                     DQINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIF
                     KGTNQYTLSLGAATYFYEDGEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSN
                     DGVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYV
                     VQGELQ"
     gene            complement(20379..21541)
                     /locus_tag="IWO38_001691"
                     /pseudo
     CDS             complement(20379..21541)
                     /locus_tag="IWO38_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
     gene            complement(21547..22236)
                     /locus_tag="IWO38_001692"
     CDS             complement(21547..22236)
                     /locus_tag="IWO38_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAQ0313782.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGHQKDKIAEKVDGVTK
                     TIHIQEQDKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYPDAEMGNGNYILAGYNVDTPSVLLSDMIDLSEGEEIDLIDA
                     HYKYQYEVSKRGKIYDYSQVVQSKENNSFLSLPKAGKKPLLTLLDFEANNMKKCDVIQ
                     CELVNVEKINK"
     gene            complement(22363..22557)
                     /locus_tag="IWO38_001693"
     CDS             complement(22363..22557)
                     /locus_tag="IWO38_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313783.1"
                     /translation="MEEKLLKTMKQKHLKRLSVMQYISDMQITGKEKACLLGSMKNFE
                     QLRRTYVKIRSNCQLLLEVS"
     gene            complement(22667..22906)
                     /locus_tag="IWO38_001694"
     CDS             complement(22667..22906)
                     /locus_tag="IWO38_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313784.1"
                     /translation="MARPVLDAMDMDTLTKGRYVQLLARSYSPKDVFKALSKGTDMEK
                     ERLRKEFDYWRIYNGIKDLKPARPVVKRKKKITQE"
     gene            complement(23073..23414)
                     /locus_tag="IWO38_001695"
                     /pseudo
     CDS             complement(23073..23414)
                     /locus_tag="IWO38_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
     gene            complement(24033..24365)
                     /locus_tag="IWO38_001696"
     CDS             complement(24033..24365)
                     /locus_tag="IWO38_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ0313785.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            complement(25190..26230)
                     /locus_tag="IWO38_001697"
     CDS             complement(25190..26230)
                     /locus_tag="IWO38_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ0313786.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT         7860 a         5387 c         4150 g         9614 t
ORIGIN      
        1 tggcactcat caatcatcgc caagaagcgt ttgaagtctt gttcttccat ttgtttttct
       61 ttaattaaac gtcgttgtct tcttccttga atcaatgcct gattccaaat caacgtcata
      121 gccatgtaca tttgacattg ttgtacccct cgatctaagc ctgtaataga gtcaatgtca
      181 aaaaagacca actgttgttg gtcaaggtgt cgaatcgtac tatgcccatc aaaaatcatg
      241 ccatacgtct cacataagga ttctacgatg gattccattt cttcttttcg attctttttc
      301 tcttgggaag cacctggtaa ttccagttgt cctaaaaagt ctaaaaattc tttgaacgtc
      361 ggataagctt ctggatcttt acctgtgact tgcgtctccg tatttgaacg cgaatttgag
      421 gaagtaaagc cgcgtggtac tgttccatac tcttcataaa aacgatttaa gtaggtctta
      481 aattctgctt tcatcgttcc ttttagctga ggactcacta actgtacctg atagaccact
      541 ttgtctaagt gtgtttgata ggacgctgct tcattgatct tcaaatcttt gactgatcca
      601 tcgtcatata aactggatgc ttcatcatac gtccgtgtgg caaatatctc taaaggattg
      661 atcattccgt cagatccgtc taaacgcacc ataataccgt tatattcctt taagaaagga
      721 ataaagtctt ttcccttttc aaagccccaa ataaagtctc ctgcagctaa atgggcttcc
      781 gcgatcattt taagaacggt cgatttacca gctcccatca ttcctagaag caaggtatta
      841 gtagacaacc gtacactatt tttatacgtc gcattccaga caaatgggcc gcctgtcatg
      901 gtttccccat attgtgtccc tcctggatct tttagcgttt ggtggtggaa cgggaaacct
      961 ccgccaatgt ttaacgatgg tagtgattgc cctttgatat ttccgaacca ttcagcttgt
     1021 tcatcgaaca tggtaaacat cgattgccaa tctcgtttgg ttttatgcag ataagtcacc
     1081 gcacgataat ccatcccttt tagattttcg atcagttcct gcgctcgttc atctacttct
     1141 tgaatcgtgt ttccgtaaac atgtactctc actttgactt gcttagaaat ttccccatta
     1201 gaagtcaatt tacgagcata ttccgctagt tccaggtatt cttctgcgct tttatttcgg
     1261 tcagtcgctt ttcgttcatt atctgatcga tcatttaatt catctaaggc acgattgatt
     1321 tgatcaatca ctttttgctt ttcatctgtg ctaacatcga gtgtggccac tgtatgagga
     1381 ttgttcatga gattggccaa ccaaaaaagc gtaggatttt ttgtgtagtg ataaactgtc
     1441 acacatcgtg aataaccatt accatacgtc acatattctt catcaaaatt taacccacct
     1501 tgtggcatca cttgttttaa aaaatctaaa tcataccctt tggattttaa aatttctatt
     1561 tcccgatttc ctagttgctt taatggatca agtacttttt gatcattttg tggtgaaaaa
     1621 gctgttttaa ttgcttctaa catgtacagt cacctcttct tgtttgattc tttacttggt
     1681 taaaagattg cccttttccc aacttaccca ggcttaaagt gtgaggaaat tttctagtgg
     1741 tctcttcctt cggaacatct ttttctaacc atatcaacgg atatttcatt ctctcttctc
     1801 cattcctact catcattcat attgttcatt cgatacagaa tcttgatcaa ttcttttttg
     1861 tagagtggtt ttaaccctaa aatgccacta gatcgcatta actgacggtt tctggtttgt
     1921 aaatccttga ttgtctttcc ttcaatgacg aaaaagaagc ttaagtcagg acgttcttgt
     1981 tctgctcgtt tcaattcctg gatttcttcg actaatttct tttcatattt ttttaatcgt
     2041 tgtagatttc ttcctaagga ctgttccata cgatttttat ctaataattg ccgcttttgc
     2101 acgatttgtt ccgttagatc cgtctctgtg gtcaaactaa ctaacttaat tgttggcaca
     2161 tacgtacgca tgacggtggt caactgattc attcgttcta gtttctcttg ttcatttaat
     2221 ccttccacgt ctgtagaaac tacacgataa taacgttgaa aatgatcttc tctagtaatt
     2281 aagactccct cttctgtaca ggatttatac ggaaataagt ccaagataga gcgctcttct
     2341 ggttttccaa actgcaaaat ggatttcctt tcaactgaat tttgtccttt gactcgcatt
     2401 tattcatcct ccttatacgt accatgataa tttcggttag gatctaacgc atgataatgg
     2461 ttcttgtccg caaaaaaaat agactcgata atcgcaaaaa tttgtcgttg acctgggtta
     2521 ttatagggtc gccaaacaat cactaacgct atcgcaatcc ataagccata attcaagagt
     2581 gcaaaaaaga gtggtaagtg gacaagatta ttgaccattt tcgcccaaaa aaagcagcca
     2641 ccaagcgtta atagatctaa aaagaaaaaa gcgaaaaact tccattctcc actaaatctt
     2701 ggagggtttt tcatgtttat atatttatcc ataattgatt tctccttttc atataaataa
     2761 aaggcctaac ttgtaggcct tttatggttt gatatcctgt ttgatatccc tcaatagttg
     2821 ttctttcaat gcattgttcc tgtttttagt ttctcttgtc acatttattt ttgttggtgc
     2881 agtaaatgcc acatttgctg gcttgtaacc tgtatgaacg ttttgaatag gttttggtgc
     2941 agcttctctt gtcacaacat tttgaacgga cgcattccct gtctcaccac tttcagataa
     3001 attgtaagaa acagttgtca gatttccatt ttcaggcaaa cgacttggtt tcatttgaac
     3061 agtacctgca ggtgattgat gaacaaaact ttgatcatta tccttgaatt gattcaaacg
     3121 actatctttc attggtaaat tttgtgcctt aggtaaacca attttcgttg atttttcagt
     3181 tccaacttcg attccattat catttggcag aacgatggaa tcctcatccg tttgtacttc
     3241 ttgatttcgt tttggtaaat ctgaaatgcg tttttcattt tcggagttaa cagatctata
     3301 ccgatattgt tctggatcag tttttggtaa ctgtccctca ttttctgctt tgtcttcttg
     3361 atttactttt gataaatctg aaattcgctt ctcattttca aggtcactgt taggtttata
     3421 gcgatattgt tctggatcag tttttggtaa ctgtccattt tttccttctt ctgaatttct
     3481 ttcttctatg gaatttttct cttcaatcga attttcatta gagtgcaacg cttcttggtg
     3541 atctttgact gaaggaatca ctataccttc ttgattttct ttttcggttg ttgtattagc
     3601 ttttactgct gcattaactt gtccttcacc atacgcatct tttagtcctt ctttgacttc
     3661 tgaagctttt tctttttgtt tttctagtgc ttcggatgga ttcttcacag cttcagccaa
     3721 ttctttagct ctcgtgccgt ctaatttctc ttctaaggtg tctttggctt tttctgtaag
     3781 atcttcagct gttcctttta acccacgttc attgacgtaa ccaagacctt ctgcggcttt
     3841 agaaacacca tttttcgctt tcccgaacat ttcggaaact gttttcttac tattaggtac
     3901 agttgtagct ttatcgtctg attgttcttc cccgttatca ggggtaacat cttttcgatg
     3961 gccaaatcca tttttacgaa tatcatttaa atggcttaaa ccactcttac cagcacttgc
     4021 aaggttttta gctccgctac ccacattttt cgcaatattt cctaaggcaa acatgctaag
     4081 aagagaatta ctgccgtttt tcagactcgt atcaacaccg aaaatcttcg taaccgattg
     4141 agagcctttg aatagcgcaa tagttagcac aattaaagca acaacataca agaatccatt
     4201 gatctttcct gccgcatgca aatcaccgat acctgaaagc attaatgtat agattcgtaa
     4261 ttctaaaatc gtaaacgcca ataacaagaa agactgagaa atatcttcga tagcactttt
     4321 catccgttgt cctgtctcaa agtccgttga cgcggcaatc actaaataga attttttaaa
     4381 taccagctca ataatgcaag taacgatcgt aaataggata aataaatagg ccaccgccaa
     4441 agaagattca ccagctaaaa taatacctgg actactaatg tatcgacgat atcctgaagt
     4501 atatacatat ttggctaatc cttcatcact aatctccaca gggatttctt tcccattatc
     4561 atccagttcc aatcggtatt gtaatgcctt tagtcgatct ttttctatat ctgataatcc
     4621 acttttatca ttccccgcca tcttagcggc ttccttagga ataatcactg aattaatatt
     4681 agcagtttta aaatttttcg ctgttaaagc atttctttta tctgttggta aggattctac
     4741 attattcggg tcagtcgcta gtaatgtact tacatcgatc acattatttt gaataatttg
     4801 aaaagcaggt gaaccagttt tatctgcttt atttgctgca gtgacgtctc cgtaaaagtt
     4861 tttactgata tttaaccctt catcgattaa agaactacca ccaaaaatca agatcatggc
     4921 cataaataga ttaaccaaga tatttttgac ttttggtggg tgtttactca taaaaaattt
     4981 caagcctaaa taaattaatg tgacgacaca aaaaagagcc aataccttac taatcaaact
     5041 ttgaaacagt tcattaacac cagcggcgtt caataagtct tttaaatcaa agctttgata
     5101 aattgttttt tctaataaag tattgagatt atataaccct ttgatcaatc cccataaaaa
     5161 agtattacaa atccaactga atacatcttg atactgcaga taattaccat aatcttgtaa
     5221 gactttcgtt acggttttgg gatctgcatc cccttctccg aataagcctg accaccagtc
     5281 ggctatgcca gaaaaaattc ctccaatgat cattctttca ccaccttttc agattccaat
     5341 aacaaccgtt tcccaatcat ttcagaaatt tcttcatttt tgatcaactt ttcaacatat
     5401 tcctgaagac gatccgttgc ttgttcttcg taaaattgaa ttaataattt ttgggctgtc
     5461 tcattttcaa gagattgaac aattttataa aacatgtttt cttcgatttt ttctcccaat
     5521 tgttcatgca gtccatgata gagatctcta gcatcttcat ctggtaagaa ataatcataa
     5581 tcaatctgta aggccttcaa atcgagatct gcgtgagtac aataaatatc aatatcatga
     5641 atggttcgac ctaaggtatc gaaatcttca agatattgat aagcataagg actattggat
     5701 ttttcagaat taaaaattgg atacggcctt atcttattcc cttttagatc tcgccgtttc
     5761 aatggcgcaa gtacaatcgt ttctccttct aaaaaagtag aaattctttc aggagttaag
     5821 atacgatctg aatcaacatt acgattgata tgtgtattca tgcctaaaga attgacagta
     5881 ctattttctt gttcaaccgt tttatttcct acttttttag agaattcttc aatggtgtta
     5941 tcgtctgtag acataatata tacatgtgtt tgacagtttt ctttgataat atctgcatta
     6001 tcttccccat acttcgcacg tagttgttta tatgattgaa taaatagatc ccataacata
     6061 ttacgacctg ccgtaaccgt catgattcct tccatgttat ccatcgctgg catattccca
     6121 aattcatcta aaatgaaatg aattcttcgg tgacatttat cacctttggt cttaacacat
     6181 tgcgtggata actctttata taactggctc agaaagatac taactacgat gtggttagat
     6241 ggatcataat caggtgtaag cataaaaata gccattggtt tatcggtata gtgcatccga
     6301 atagactcta ctaataagtt acttttactt tcatctagtg tgagaaccgc ttctttttcc
     6361 cattctggca aatgggtttc tccccttttt tttcgataaa cgatattacc ctcttcgtct
     6421 ttcaattctc tttgagaaaa ctcataccaa gcaacatatt cttttctttg gtgatcatac
     6481 tttaaacaaa cttgatcccc tttctctaaa tgacaatcaa agttgatttc acaaaaacct
     6541 ctataaccaa cttttactcg ttcttcatga aatttctttc cttgatgata aaattccact
     6601 acgattcgtt gatttaaaag actttcatgt aatttcaaat ccaaatattt gggaaaacca
     6661 accgatttat aatcaatggt tgattgagaa gtcattttcg caatttttgt tagggtaaat
     6721 ggattcaatc ctgtgatcgc tgttgataga atattgcctt ttgctttatc agaaccaatt
     6781 ttggtcgccc catattgttt tttcgctaga tttccaggtg gtaactgatt cataaattca
     6841 tctaaagcat tgagttgctt aaattcatta gtaggatctt gataattaaa accgccaatc
     6901 tcagaaagaa gttcgacgag attattgggt gttaatttgg agtaatcccc tgtttgatcg
     6961 gcttgttcta acaacgcaaa aataagcgca ttgacagctt tctgcgcatt ttcgttaaag
     7021 tatttgttgt ctgaagcatt ggcatcataa taaatcgtat ttgtaaatgt atttgctagt
     7081 tgcgtagcgg tatccacgtc tcctcgttgc caatatttca caattaaagc caaaggatta
     7141 tcactcattg actgtaacgg ttcagttaga ttataagcat aaacatcata gcctctggct
     7201 tctaattcgt ctttgctggc tacatacaac tcatttttag gatcattgac aaccattgaa
     7261 ctttgttttt ctgcacgaga caaattatca atagttgcta atacttcaat ctgtcctttc
     7321 cctgatcgag aagtaccaac aatacaagta ttaacggtat ctgtttggat ataccaatat
     7381 tttttatagt gagtgatagg aattccacca tatccaggga aagaaccttt cgcgtctggt
     7441 atttgaggat agatttgttg aatctctttt agtgtcatta accgattatc ccctttttgt
     7501 ccatccgcta gtggacgaaa ccacatgtcc attttcaagc ccataagtac agctggaaca
     7561 acacaaatcg tagaaacaac taagcgtgta cctaaactcg ttgctggggt caaaggattc
     7621 caaaaaccag ctgaatcaaa atgaaagacc tgtggcattt cctgtaattg aaacagattc
     7681 actccttgta agcttccttg aatgatttca aatcgactga aagcttcagg tattcttgtc
     7741 actaaataat ctaaagggag atagactaaa ccgctataca ttagtttttg tttgactcgc
     7801 ttatcatgct tacctggtaa tcgttcacct gctggtgcat agctataaaa atgtgataga
     7861 tatttctttt tttctaaatt aaaaggcttg tcttttcgtt tcgttgtttc tcgaatccgc
     7921 attatttcac ctcatttgat tctgaacttt cggaagagtt tgattgtaca gttgaatcgc
     7981 ttgatccttc tttggtctta ctagaatcac tcgctttgct gttttccata cttgactctg
     8041 ttgttgactg attggtacga ttcaataatt cttctaattg ttgttcatac tcttttcttg
     8101 cttgttcctg ggcttcttcc ttgattgacg ccgttgtggc tacagaatca gcctgtgaag
     8161 attgattgat atgattaaaa gcaaatactg tccctccacc tagaagcaag cttaaacttg
     8221 caatacatcc caccatccat ccgttaaagg aacgctgtgg tgaaggagtt aaatttgctt
     8281 ggttgttcac tggcgtttga atagcagcta ctggttgagg ttgtgctaga taattcaact
     8341 gtgcttgacg caatgtatcc atttgttctt gacgttcctt acgagcagct gcttcttttt
     8401 ccttttcttg ctctaaccgt tgtttttcta tcgccatttt ttgttcagca agatcctgct
     8461 cacgtttttt tatttctaat tgttcgcgct ctttttcttc ttggcgttgt ttctcttctt
     8521 gttctttcaa ttcttgttgt tgagtttcat actctttttt ccattttgga atttgtgctt
     8581 gtaattcttg ctggatctgc tcgtgttttt ccataagaac ttcagtcgtt gtttgatata
     8641 gtttattcag ttgcccattt aattctttgc taatttttaa cttatctgta cgtaaacctc
     8701 ggtagaactc ttcttttcgt tctttaattc gttcttttaa caagttttct ttttcttttg
     8761 atagcgcttc ttgattcgtc ttagtaaaat tgttgatctt ttctacacac aaggaataag
     8821 cattgtcgcg tttcttttcg taccgctctt tgattttttg tacttcttct tcaatcactc
     8881 gttgttcttc tttatttagt tcctcttttg ctctctccca attcgacgac tgttcagtcg
     8941 catactcatt tatttcttga tctgtttcct gttgacgttc ttcaaaagta gctaataaat
     9001 cttcttgtgc aagctcatgc cagtttgttg attggacaga ctctaactgt tctaaaagtg
     9061 tgcctttacc ctgtgagatt aattcttctt tactctctaa aaatgcttga taggtttggg
     9121 gtaatttata tttcgaataa agctcattta aaaataattt atttttttct ttcatccatt
     9181 catgctctgg cggatattga tccaaaatat aatcaaatac agttgccttt tgtatctctg
     9241 tttcgttttt ctctacttct tcactgataa gtggttcaat atattcctga gaatcccctt
     9301 cagtccatgt tggctcttca ttagcttcaa ctggatcaat ttctacttcg gccgtctctt
     9361 catcctccat tgtttcttca aagggatcta cctccaattg atccatcacc tcgttctctt
     9421 cctcaaatat cgatttatga gtcgaaatgt cagcttgttc gtcagcaata cctttctctt
     9481 tttcttttga ttcatccttt ggaaagattg ggcgttccca tggtacttct tcgtttgctt
     9541 cgacttcact cacttgattc acttttcttt ggtcggtctc taactcttct tctaaaactt
     9601 gtagaagttt ctcgttacga taagactctt ccacactcat ggtttcatcc gtttctttaa
     9661 acacatccag gcggattaaa tccatcaagg taggatagaa accttctgaa atttgataat
     9721 ccagcggccc aaaaacgtat tctgtaccag ctttctctat ttttccctct gtgtcatgta
     9781 acgtgagagt caacttaaca gaagattgac tttcttgttc cagtactttc cctaacacat
     9841 gtagctgttc ttccatttca atgacagaat atttaattgt tttcggtaaa aaagaagtac
     9901 gctttcctac ttccttcgtt gcttttttta ttacttcttt atctgttttg gtattgcctg
     9961 caatcgtaaa gtacatgaat attctccttt cataaaaaag gagtaaaaag tatcgactac
    10021 ttcttactcc tactttttta ctttcttata atccttgagg tgtttgccca aataatccaa
    10081 ataacgaagt tatgatagaa gcagccccta gaataacacc tacccccatc aatacataac
    10141 ctacgtgcat cttcccgttt tcagctcctc ttcgtccaaa taggaagaaa agccccccaa
    10201 taacaatgga agcaacggcc gcaacaccag caaaaatcat caaattattt tttaaactag
    10261 ttgctgcagt ttgtaatgct ctaaaaatat caaccgcagc tactactttc ggttgtgtta
    10321 aaaaattcat tgtaattgat tcaaacataa aattgttcgc tccttttagt gtttaataat
    10381 ttcatcgaaa acaagctttt cacgttcacg ttgatagata tatccttcta agtccttgtc
    10441 ttttccccgt aataggtcaa attgtgcttt ttcaaggact tcttgtttcc actgtgtttc
    10501 agaaacacca cgacgttcta agattttaga cgcggctttt tcaaatggcg ttttagcttt
    10561 agccactata attcctcctt tagggtaaat aaaaagacca gcaatcaatt gctagtcttt
    10621 tgtaatttgt atttatttaa ctctgatcca tcatgaatca atttctcttt cgttcgcttt
    10681 tcatccttac tagatccgag caaaatcatt ttttgtacgc catccttctc ataaggaaca
    10741 acaataaaga gataatctgg tttattgata aattcgctaa cacctgcaga atctactact
    10801 ttatatctct ctaacccttg ataatctttt tgtttttcta cacttacctg tacaggttct
    10861 ggattcattt cttcagaata ttcccaagta gtatcatcaa ttattttaag tttataccaa
    10921 tttccaccta gttctctttt ccaaacagtt cctggttgca ataaactatt atcttcttta
    10981 gcttccgatt tcttattact tgaacatcca cttaaaataa taaaaaatat tgcaaacata
    11041 gagacaaacc atagttgctt tttcttcatt ttattccttc tttcgttgtt tatttttagt
    11101 atatcaaata acgaaagtag caacaaacct cattttgata cacggggaac ggatactcat
    11161 aatttcccac ctttctatag aaacttttcc tcttgatatt gtgatttatt acaaaaaaga
    11221 gctataataa agaaaaaatc aagagggagc attgcaatgt acgctgataa agagaaaatc
    11281 gaatggttac tatttgaaac tggcaagaca gcatatgaaa ttagcaaaca ttcttcagtc
    11341 gcagaatcaa caattgcgga tttaataaag aaaaaaagtg cgattgaaaa aatgcgattt
    11401 gataatgctg ccaaactaac caaatacgcc gaatccattt cttcatagca tataaacagc
    11461 catccttaca cttttcatag gacggctttt ttatttcaaa atttccacgg tataccgttg
    11521 actgcctcgg tataccgtgg tatattatga atgtaaacaa aatatttaat catttaatag
    11581 gagttgaaaa catgaaatta ttaaacccaa aaactgttat agctggaaca tttgctgtca
    11641 cacttgctgc cacacttgct cttgctggat ctgttgacgc tgcttcaact agcgtcatgt
    11701 atcgccttta taatccaaat aaccatgaac atttctacac ctctagcaca aaagaacgtg
    11761 atcacctagt gaaaattaag tggggtaatt atgaaggccc cgcttgggaa gcaccgacta
    11821 gcggtggtca tcttgtctat cgtttataca ataaaggtct tcgtgatcac cattacacag
    11881 ctagttggga tgaagtgaaa tggttgacta aaaattacgg ctggacttat gaagggcctg
    11941 cctggcgttc tgccgaaaaa aataataaac cgatctaccg ccttttcctt cctggtgtaa
    12001 aatctggctc acatcactac actgcaagtt ggaaagaagt acaatggtta accaaagact
    12061 atggctggaa atacgaaggt atcggttggt atggcgctga cacctctaaa cccaaagtga
    12121 ataaatcagc tttagaagct ctttatacga gcgttaaggg aactaaaaaa ggtgactata
    12181 ccgacaatac ctggaacgca ttccaaaccg ctttaaacaa tgctaaaaaa gtgttaaatg
    12241 actcaaaagc gactcaaaaa caagtggata gtgccaaaaa cacattagat tctgcttaca
    12301 agggattaaa gaaaaaacca gctcctactg tgaacaaaac ggaattacaa gccttgtaca
    12361 accaagtaaa atcaactgct aaaggagatt ttacagaagg atcttggaat aattttcaga
    12421 ctgctttatc caatgctaaa aaagtactag acgattctaa agcttctcaa acccaagtaa
    12481 ataatgctaa aaatagtctt gattctgcat ttaaagggtt acaacacaaa cctaaacctt
    12541 cagaatatgc ggtaactgtc cgtcatatga atcgacaaac tggaacgctg ctaaaagaag
    12601 aacatgtctc agtaaaagct ggtacaagtt acactgcaaa agctagaacc gacttgacta
    12661 actttgaagc accacagtat caagtaaatg ggaatgaaac acaaacacaa acgattaaca
    12721 caaataccac atttactttt tactatgatg aagtctttct agttgaagtg aaagctcgtc
    12781 caaataaagt ctcggatact gatccaaata tccaagaact ctataattac agtaagagtt
    12841 atcccgtcgt ttatggacaa agcatcacaa ttgaagcacc tacgttcact aattttgttc
    12901 ttgatcctag agaaagctct agcaacactg tgactttaaa caacgttaca agtaatcaag
    12961 gaatcaattt tgcctatact catcaataca acgtcactgt gaaccatata aacgttgaca
    13021 caaacgctgt tttatctact gaaacacaaa cggtttacga gggagatagt ttctctactt
    13081 cttggaaaaa tatgaccgat caaaactact tcttgtgtag aaatgacgat tcatctgtta
    13141 ccgttgataa aaatggacaa cgtgtaatca ataatgttga ccaaaatcga acgatcactt
    13201 tcaaatacaa aaatatctca ctaagtgatt taaacaacta tgtgcgtcaa aaagaacttg
    13261 cttggttaaa tagctaccgt caacaaaatg gcgttgcccc aatgcaattc aatgatatcg
    13321 ttcaacaagc tgcagatatt cgtgcaaaag aattacaagt aagcttcagt cattaccgac
    13381 ctggcggcgg tactttccaa gatctcttag aaagcttagg ctgctacggt gctaaaggag
    13441 aaaatatcaa tagtttctac ctttacgttg acgagatcct ttcagataca ggagcagtaa
    13501 atgctatggt tatgtggaaa gatagcgctg ctcataacgc aaacttgctt tacaacaatc
    13561 aaagcatcgt tggtttatcc catgcttatg aagtttctgc agacggatca ctttcttcag
    13621 acaacgtatt tattagtgcc aatccaattt tcaaatagaa aaacaaggct gattttcagc
    13681 cttgtttttt tgtttaattg aacttcagaa ctgcgtagct aaaagttgct aacacaagta
    13741 atagtaagct gaccgctaaa atcgttacta aatgtgtaga acctgtttta ggaacaataa
    13801 cttgtggcaa gttaggatca accccaattg tcactacatt cgttttatta gaaacattat
    13861 ctactgccgc tacagcttgg ttatcaatat ttttttctga ttgtaatgga gttttagctg
    13921 taaatatcag ctgaatttct cgtccatcta aaagattgtt acttaagaag tcagcggaag
    13981 cttcccactt cagcgttctt gaattgctat cataggttag tgtgccttca gaagtaatat
    14041 cttttcctgt actcttatct attacttgcg cagaaccttc tttatacgtc aaacgagctt
    14101 caatcggatc ttgaatacta aattgcttgt agtgatctga cgcagcgtct acgccttttg
    14161 tatgccattt ttgtgtgact agatattgta cttcagaccc tgcatttaaa ggagctgtcg
    14221 gtgtaccctt cttaccgtca tttcgtccgt ttacatcttt tccatcttgt tgcacttctt
    14281 tcactggaat tttagggaaa taaacggtaa tgatatttgt atccttttct actttattaa
    14341 tgatcgaagt tccctgattt ttggctacac tattttgatt ggccaattca ttgactttta
    14401 cattgatcac taattcgtaa gtttcgccgt tatacttcat tcgatttttt aatgtatcag
    14461 ctttcgctgc atattttacc tggtgttttg ttttgtcata agtcacttca ccagatacat
    14521 cgaatttttt atttgatcca cattctacgt gtgcatcgac gtaatctact tctttaggta
    14581 aattatcaat taactcaaat cgatcgtaaa ccgcaagtaa atccactcct aaacgattca
    14641 ctttttgttt gattcgatat tgaattgtat ctcctacttg caacatttta tcgttcacgt
    14701 tatttccatc tttatctacc cctgtttttt caggttgatc aggaacaacg ctaaagattg
    14761 tcgcagaaga taaagtaaac caggtttgaa cattagaaga accaatcaca aacgtcggat
    14821 ttgcttcaga tattttaaag gacaccgtcc cttgaccaaa cttcggatct cctaatttat
    14881 catgcaaatt gttgaaatca ttatccgctt gtgtcagctt agagcttcct cctgggtgac
    14941 cacctactac gttcagattg ttgtagaaag atttaaattc tgaaagaacc gtatcttttc
    15001 tgacatacca atctgtcgta ttcattttgt catagtgggc atattctcct ttaagatccg
    15061 tgctttttcc tcctgcaggg ttcaaagagt taaaactgat aaatgtatcc cctgaaaatt
    15121 gaacgggact accgtcctca tatgtggcta ctagctgcac attcatttgt gttgaatggt
    15181 aaacaattcc tgagaaaaga ttgtctgaga tccaaaggac tgttcttttt ttagatttac
    15241 tcattgcacc attcgtattt ttatagccat cgtctcgaaa atgagttttt gtatagttat
    15301 tatctagtag attccacggc aacgtatctg aatataagtg gatattagat agaactagct
    15361 tgatattcac ttttttcccg ttatacgtac cgatttttcc gtagtcaata ttgactttgg
    15421 tgttggttgt tacgccttct aaaacaaggt ttgtttcacc atattctttt tggaaagaag
    15481 ggtaacgtat atttttgttt ccctccacta atcctacgta gtcgtctgta aaaccacctt
    15541 tgttggcatc aacatgatat ttgtaaactt tggacgctcc gcttacagta atatttgttt
    15601 ttgcattagg tacatctttt ggaataataa aattattctt tacgttctgg attggatttg
    15661 acggatcgac aataactttt tcagcaaaga cattggctgt ttttaaccca aacaatgtac
    15721 ctaacatcat cattatggga aaaacatatt ttaaccgtct tgtccacgag tgtttaaaat
    15781 tcatcccttc atcccctctt ttttataaat gactagtgaa attccgacaa taacgatcaa
    15841 attgatcgta gtaaataatg gattcctaat gagcttttcc ataatatcta gcggcatgga
    15901 agcagctggt atgtttaaaa tcctcattag gtatagttgg tagcttggca aaatagatag
    15961 ttgagaaaat aataaaccgc agtacaaaaa gactgcggtt aaacaagaaa gattgaataa
    16021 taataattta atcagtttca tcttatttct tccttaatct ctagaaaaca agtgagtaac
    16081 aaaatcccaa caattctcaa agaagttttt ctttacgcca gtagaaacga ttttttttac
    16141 ttcttcttta ggttgcgaag cttctgattt aggatagatc cacacatcct ttaagaattc
    16201 accgttttta tcagaaacag gtgttaatag caaaatatcc tcgctattag aaatgttttc
    16261 aggacttgat tcattgacaa tataataagc ttgtttctga ttagcctgta cttctaccga
    16321 gaattcaaag acacctgctt gttgatcaat tgtctttgtt tctccattag aaactagctt
    16381 caattgatca ctggatagtt ttttcactcg atcttttaat tgggattcaa tttgatcagt
    16441 tgagagatcc ttaacatcca tcttttgtaa aatgtccgat acatcataaa cagaaaactt
    16501 cgcgccattt actccttctt gtttttcaag cttttgcccc tggttgtcta attgttctga
    16561 agaaataatc ttttgaacgt gaatggtctg tttgactatt tgatcgtctt cagcaatttt
    16621 aggttcttct gaagaatcaa ctgttgacga atcgacggtt tcatcgctgc tttgaatcgt
    16681 gctgctatct gacgtttgga ttgtttgttc tgtgctatcc gtgctttctt gactgtctgc
    16741 aaccgtcaac tgtgtgtagg cagtgaaaga aaaaagagcg gctgcacaaa caaatagcca
    16801 ttttctattt ctcatgttgg ttaccctcct ttattttttc tttttataaa caaatagcga
    16861 accaataatc atgactgaaa gaaaaacagt acttcctaaa agccatttcc aagataattt
    16921 ttgtttttta ttttcttcat cttttaattc tgattttgta aaggatactc gtttcccata
    16981 cactaggaaa cggtgtgtgt tgattccctt aggcgtacat gtcattagcg ttactcgatc
    17041 ttcattggga tttggttcta agtaagtgac aaagtttctt tgtagttcat ctttttgcac
    17101 ttcctcaatc cgatcgattt gataagcgtg ctgctcacca tcaactttga tataaaactt
    17161 ttctcctttt tttactgaag gtaaatcatc aaaaagatta tctttataaa gtccactatg
    17221 acctgcgatt aaaggatttt taccgtttcc tcctgtaatg tcaccagttc cttccgtaat
    17281 acctacacca ttttctaaaa ttttgtcgga cgttccgtca taaattggta gttttaaaga
    17341 aatacttgga attgtaagaa taccaacggg atcgcctaac tcaatcttag caacatccaa
    17401 agaagaatcc gtgcttgctg ccgttcctgt tcgatcgatt gcggcaatct gctgattttg
    17461 ctggtatttc gccacgctcg cttcagcagg catcaaaagt aagacacctg cagctgaaag
    17521 taaccagaaa gcaatgatcc atttacccat gattcgcttt ttcttaggct tcctgataat
    17581 cattggtttt gatccttcta agctttcttc ttaaagtaaa caactgcaga aagtgcccct
    17641 gctcctagca acaagcaaga gatcaataca tacgtttgga aaccgttact accagtcata
    17701 ggtacggaag ctttttgatt gtttttgatc gtgattcggt tttcttcttt gtatgaatct
    17761 ttgtctactt tgaaagagat ctctttcgtt aatttttgat agttttcagg cgcttttgtt
    17821 tcttccagtg aatattcacc ttcagttaaa ccagtgattt caaagtgacc ttctttatct
    17881 gattttagga tcgctgcatt tgctacatcg tctacccata ctgcttcttt gacaccgtct
    17941 ttatccgtat caactaatcc accataatat tttccttctg cgtttttagc agcaaattct
    18001 gcgccttcaa gcggtgttgc tttgattcct gttccaaata gtccgctatc gacttttaag
    18061 aattttgcac cataagtcac aacgtcagct ttcgtttttt cgtggtgttc ttcctgatta
    18121 aatggattgt tgttataaac gaaatccacg ctatttgtta atggattggc aacagctgcg
    18181 ctgtcattta catacatgtt ataagtcatt tgaagatctt gtccgtgtaa acttgctaca
    18241 gtatcactta cttggccatt attcacgata aaatcgatca caaagccatt ttctgaattt
    18301 tggatcttat aatctgtatt ttcttttaat accacagatc catctttatt tgttactttg
    18361 atatttgctt tagcagcgac ataagaaagt ccttgtcctg ctgtatcccc ccatttaaat
    18421 acactgtaag gagaagtttc gcttttcact ccgtttactg tgataccgtc tgctccgcct
    18481 gcaatgtctg ttgggatatg aatcgttcct tgataattta ctgcgtcacc aatttggaaa
    18541 gaatgttctt gtcctgttcc atttgttact gttttatcga tcactggtgc tttatagttt
    18601 acgtatgaag cttttaagtc acttgtcacg ccatcattcg taatttcaaa ggttaattta
    18661 ttgtgcgcat cgttgcgagc gtctgcgcct aataaatatt gatctgctgt tggttcttta
    18721 tctgatccca ctacaacttc tgaaggaact tccacaaaat agtattttcc taacgtcaaa
    18781 tcagtagcag tcagctttcc ttgatcatcc gatacaagat ctcctaattt tgttcctttt
    18841 ccaggttcgc ccttgtataa ctcaaatttt gcccctttta aaggtgtttg tttgctttga
    18901 gctgtgccat cgtcaccaaa tttcgttaat tcaaaactca acttagaaac aatattttta
    18961 ggatataagt gaatgtcttt taaaaaacct gaacctgtat tatcagtcat tggcgcaatc
    19021 accaccattg gttttgcttt ttgtgtgact aatccagcag cggatttact ttcataaacc
    19081 acatatacat tccctttgga attgacatag gctggttgat cagcaaaagt gatctcacca
    19141 ttttcatcta cagctgaagt aattttgttg gttgcattct tgacgtattc actgttagat
    19201 cccttgtctt ctacatcttt aacgatttcg tccactttcg cgttggttag atcagaatct
    19261 tcagtcggta atacttgatc cgtaatattt cctaacgtaa actcaacatc accataatct
    19321 gccttgttgt atttcgttac acctgtagga tattgatcgt gcgtataacc atcgtttttg
    19381 attccgtcaa tatcaacatt caattgcgtt ccttgatcgt acattaattt atgaaccgtg
    19441 atattgacct tctctggtgg cgttttttgc gtgacatctt ctgcatgtgc ttgatacccc
    19501 tgaaagcagc ttgcaatgat tcctaacatt ccaacaagca ttccagcttt ttttacatac
    19561 cgatttattg ccattttttt atctcctcat ttcatatttc aacgactcgc gttgattaaa
    19621 accttgttat tgtagctctc cctgtaccac gtatctttct tttccttgtg acgtataaac
    19681 acaagtaaac aacgttaata agggtttctc tcctttcttt ggcaaagaca agaaagagtt
    19741 ctcttcagga acgccatcga ttaatttaaa gtactcaggt acgataaatt tacgcgttac
    19801 cttgtaacga tagacgccat cgttactaac taagtccatc acctccccta atgacaactt
    19861 ttgaatatca gaaaataaaa cacctggcat ttccatgtta tgacctgcta ggacatagtt
    19921 tcctttgccc atttcgccat cttcataaaa ataagtagcc gctccaagac tcaaagtgta
    19981 ttgatttgtc cctttaaaaa ttggcaatgc taaaccaagt gcaggaatct gaatttgtgc
    20041 aaatacttgt ttattgacgc ccatttcgat tgcttctttt tgtatggaca acatttgacc
    20101 caaagacata tcattattga cgttcgttgc atcctcctta taactctttg cgtgtgcatt
    20161 gatttgatct acgggtatct ttttttctat attttgctta aacacctctc cttcatgttt
    20221 ttggtagcta acatagccaa acacactgca aaagacgatt aaagcgactc ctaaaaaaaa
    20281 aataaccatt ttttcatcca tctgcctcct ttcagataaa acaaaaaaca ctttttcaca
    20341 acgaaaaagt gttttctctt attactattc tatttcattt atctggtggt tttatattgg
    20401 tgtgtagcca ttgctaacat taagaataca attggcaccc aactgattgt agttaatgtt
    20461 tcttccatga atgattgaca catgatcgct aaaaaagccc acaagaatgt tagttgtccg
    20521 tttgtcagct cacattttct tacgagcaac cataaaaaac taagcaaagg aataatcgat
    20581 agagcaaaac caatcaatcc atagtgattg acgagtgcgt gatacgtatt atggttacct
    20641 agaatctgat ttcctatatg gaaaaagaaa ggtttcatcc ctacaaatat ttgtcctgtt
    20701 ttttcgccta atgtttcata gaatttgcgc caaatttctt ctcgtcctgt aaataaatga
    20761 actttgtctg acattgcagc aaaataagaa atcggtaaag ctagaaccga aactgctgca
    20821 tacatccaaa aggacaagtg tgtgtttcga aaaatcttct taggtaaaat ttctaggata
    20881 acgaagataa caagcgcgta taatgctgct ttcgattggc aaacccaaat cgttgcccca
    20941 gcaagaatca acgctggtaa tgatagcaat cgcatgtacc attttacaaa tgaactagca
    21001 aatcctgtga taagaagggc taatgtcatt aaagaactac ctatcgtatt tgtattgatc
    21061 caaatatctg ataatttgtt cgatggattc caaatacttt gtccttctgg tactggtatc
    21121 tcggtaaata ctcgataaac cgttacgagt aaaaagacat agaatactac aagatacaag
    21181 ctacgatcaa acacagtaaa ttccgtccat tttacaatca aaataatggc aataattgtc
    21241 ggaacgatca gatcaccata gaaagtatct actcggtagc ttctaaaaaa agaaaagaaa
    21301 aggtaaaaaa gaatgcctgc tgcaatgaag aataaatcgt ttctctttac cttgtacaga
    21361 ttcaaaagaa acaaacagcc tacgaaaaac gtcactaact gtgcagcatt atcataaata
    21421 atatcgtagg aggatttaac cccaccgtcc atattaaata tagaatggat accaacagta
    21481 acagaaaaat ggcactcatt ttattgatca atttttctac aagttcttga atgtgactca
    21541 cttcccttat ttattgattt tttctacatt gactaattcg cactggatca catcgcactt
    21601 tttcatatta tttgcttcaa agtctaacag tgtgagtagt ggttttttac ctgcttttgg
    21661 caaggataaa aaggaattgt tttccttaga ttgaactacc tggctgtaat cataaatttt
    21721 tcctcttttg gagacttcat actgatactt gtaatgagca tcgattagat caatttcttc
    21781 tccttcagat aaatcaatca tatcggataa taaaacgctt ggtgtatcta cgttataacc
    21841 agcaagaata taatttccat ttcccatttc agcatctgga taataagtag aagctactcc
    21901 taaactaagc gtatcgctat tcgctccttg aaaaacaggg agaataaatc ctgtagaaga
    21961 aaaaacaagt tttccagcaa tttcaccttt gagatccttt tctaatgcct gtttttgatt
    22021 agtaataagt tgtctcttag aaatatccgt taaactggtt aaattatctt gataggtttt
    22081 atcctgttcc tgaatatgga ttgtttttgt tacaccatct actttttcag cgattttgtc
    22141 cttttgatga ccttgataag caaaataact aaaaaggaga aaaacaatga ttacgcccac
    22201 gtaaggatta aataaagttt tattgttttt attcatctat ctaccttcta tatttgaatt
    22261 aatttaccta catctaacca agataaaccg cgttaaattt tactattctt tttattaaat
    22321 aacaatgcca catcataatt gatcattttc atatcataat cgtcaagaaa cttctaataa
    22381 tagttggcaa ttagatctta ttttcacata agttcgtctc aactgttcaa aatttttcat
    22441 tgatcccaat agacaggctt tctcctttcc tgttatctgc atgtcactga tatattgcat
    22501 gacagataac cttttaaggt gtttttgttt catcgttttt agtaactttt cttccatttc
    22561 tttcgacact tttttctatt ttcgatttct ttacaattca tgagttttcc taccttcctt
    22621 gttttgataa aaataaaaaa gcctatgatc acatacgctc tacacttcac tcttgggtta
    22681 tctttttctt tcttttaact actggccttg ctggtttcaa atccttgatt ccattataga
    22741 ttctccaata atcaaactct tttcgtaatc tttctttttc catatctgtt cctttggata
    22801 aggctttaaa tacatcttta ggtgaataac tacgagctaa cagctgtaca taccttcctt
    22861 ttgtcaaagt atccatatcc atcgcatcta aaactggtcg cgccatctaa tttgcctcct
    22921 aaataaattc tttactcgat gtctaatgat aatactagct ttttattatt cgtcgtaatt
    22981 tctgcacatt cttcttcatt attttctgcg attaacgcat aatagcatgg tgattttact
    23041 tcgaaaaatt tcatgatcta ttcctccaat ttttattctt ctttatccgc tacaaagtca
    23101 atgatttcaa cactgacaat ataatcactc aaatatgacc aggtatcttc tacagacaat
    23161 tcgtctcctt tgtcgttcgt aagcgtcatt ttaaaccact catcgccttc atcatcttca
    23221 ggtagcaagc cgaatccaat tggattatca gtcaatgttt aatcgtataa atcatcctcg
    23281 tctaatgcta aaacatctaa tcctttacaa tttccgcctc ttctaaacgt cacaaatcct
    23341 ttgtaatccc aacgttggaa agaaacctta atagtatgtg tgcccaatcg tatattcgga
    23401 tcataatttt tcatttgtca ttcctccatt acgtaaattt cattttctct atgttttata
    23461 tcaatttttt cactttattc aggacttgtt catattttat cgtttttcgt ttacttaaaa
    23521 atacataaaa aatttcgact cgtttatggc gtccgaaacg agaaacccta gtagacagcg
    23581 gtaaatgctc gattgatcca ataatcaacg tcctcgcaag tatctacatg tactgcagca
    23641 cacgacacaa tttcttgtgc gtcctcccca ttacgagaag acctactcct tattccatga
    23701 gtttgcatta aagtctagat cccttagtag ctgctcatga ttaatctaga cgctattcta
    23761 acttgtctaa cttctaaatt ccctcttgtt tactctgcgc ttattattta cacaaattca
    23821 tttcaccact atccaaacaa gcgcatttac tcgattgacc aattttttac gttaccaatc
    23881 gttaaaacct accccgtcac tccccgaagg gaattgttga tagatcaagt ctactcctta
    23941 tttttctagt gactctaagt tcctttagaa gtctcgacaa tattttcaag caagcgaaaa
    24001 acgataaaat acaagttatg agaatgatca attcagctga tcaaacgccc catccctttt
    24061 gcggtacaac gcaagactcc attttttctt cgaccaatca gtacaccgtt ttggtcataa
    24121 acatgtttga ttgttggata tagctctttt aaggggatag gatcttttaa aattatttgc
    24181 ttttgtttcc caatgtaata aatcatttcc tctgagactt tgtctttctg gattatatat
    24241 ttcttacact gcacaacggt tttactttgg ccagttgata gtttcttcgt gattttctct
    24301 gtagcggtac accgtttacc gtagtgacaa atatctttga gactcaaaat tcctgtatat
    24361 gacataaaat aaaccaccca atttctttaa atatttttaa caagtttttc ttgttatttt
    24421 tcaaaaatag agaattgatt ttcgttcatg acaaacaaaa aagagggaac gcatccaaac
    24481 atgcgttccc tctaatttca gcgttcgtca tacggcaaag agccacacgg acatttttat
    24541 aacattatgc ttaggcatag attcatcgta atatcacgta tttttaacct taacacagcc
    24601 aaaaaaagtg gtatcacttt tgaatatctg ttatactaaa tccgttgcgg gtaataccgt
    24661 aacactgaaa ggttaacgca ttaagggcca aatcttaatg ccgtgtatga acgctgacga
    24721 agattaccag ccttccgagt tcataccgcc tttctttttt ttgtattttc aattgtccaa
    24781 atttatcgtc caaaagtgca gaacgaaaat caattcatca caggcatagc agacaattaa
    24841 atttatacaa aaaaatgtaa gcgaataata ttcgtattgc ctgaaaagaa gtaatatcaa
    24901 gatttttagc ttgtttaaat aatgaaatta tctctcttcg cgtattatta taactcaaaa
    24961 aaaaggagta tgcaaacata cgttcccttt tctatatttg aaattaatta tttcttttaa
    25021 cacttatgac gtaaaaggct acgtttgtga tttttatcat aatattttaa cttataaatt
    25081 ctattaaaat agctattatt ttgtaatatt cgtaatataa ttagaataat aaaaaatttt
    25141 taattttaat gttactccat gaagcaacaa tcactttgtc agtaaagttt cactctaacc
    25201 agtctttcgt aatagtgctt ggggagcttt cataattcaa acgtttttca cttgatactt
    25261 tagcagctat tggtttttcg ataaaccaat tttttacata gacaaaaatc aggttctgga
    25321 tattttcgac tttttcggtt tttaattttt gataggcttt taacagtgtc gtgtataatt
    25381 cttgttctgc attgacataa taactatcga gttcttcaaa aactatcgaa ataccacttt
    25441 ctatttcagc ttttttcttt gcattatgaa tagtctggac ggttttttga gcttcactgt
    25501 aacttgaaga aaatgttttt atcaaattta atactcgttc agggacaaac gtagcaatgc
    25561 tgtcgtttgt cataattgtc tcaaaattgt ctaacaacaa ttgatcttgt aggcggtctt
    25621 tttcagtgtc tatattgtgt ctattagtgt ctaatatatt attattaagt tctggtatga
    25681 ttttcatacc ctctgattta tcaacgtttt ggcacggtat gattttcata ccctcttgag
    25741 ccccaaggga ttcagcgtct gatttttcgt cgagggtggg tatgattttc ataccctctg
    25801 atttatcaac gttttcccta ttctctctat caaatttttc gagtaagtag atatcatttt
    25861 ctgagacttt taattctgct agatatagtc tattaggatt atttttccct aactgtttgt
    25921 taaatcccat ctgcttttgt aataataaac caaaggattc caactctttt ttgattgcta
    25981 tcgctttttg tttttggcag tttaatacgc tacatagttc atttgtcgta taagtaaaat
    26041 aaacattacc ttcttcatcg atttgccgtt tgctgattgc gatctctaat cttgctttta
    26101 aaagcatata ggcaatctta gttgaatctt tcattttttt gtattcgtca gaaacaataa
    26161 agacttttgg aaattgataa aataattcgt tataaactct atccgcctta aaaaaattaa
    26221 aatcagtcat tgtagatcct ctactttcac gttgttttat ttatgtaaaa ttacctactc
    26281 ttttcgaaaa tagcaaagat aaataaaaaa tgcaaacgca ttaaaactgt tttttaatta
    26341 aatactacct aaaatcagaa ataaaatgaa ttatgtacta cctttaaagt cattaagaac
    26401 tactttaaat aaatattatt tataattaaa tactaccaga agaacgtatg aataaaattt
    26461 agttttaaaa ttaaagataa ttaagtacta ctagtgtcaa aacaaaaaat aaattaagaa
    26521 ctaccttatg catataaaac atactaccat tactagtgat atatactacc ttatttcagt
    26581 agtatatatt atattattct tataagacaa ggctttaggc tagaaaacaa tagattatat
    26641 actactaaaa aatacaataa gtactaccaa tgtcagatat atataataat tatgtactac
    26701 cattaaaatt aacttattta ataagatact actgtgttaa tctgaaaata attactttaa
    26761 agtagttaca tactacctgt taaattagat actaccatta atacttaaat tagatactac
    26821 ctatatttat attaagtact accgttctag gaataagata ataaaaaaat actaccaaat
    26881 tgcaattaaa tactacttaa aatgtaaaaa ataagaacta cccaaattag gtagttctta
    26941 ataagagaat atcagataaa tttgtataag tcataaaaaa attaatctta aatttttaaa
    27001 tatatatact t
//
LOCUS       DACECY010000038        25671 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.039, whole genome shotgun sequence.
ACCESSION   DACECY010000038 DACECY010000000
VERSION     DACECY010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.039"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            170..514
                     /locus_tag="IWO38_001698"
     CDS             170..514
                     /locus_tag="IWO38_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ0313787.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            515..934
                     /locus_tag="IWO38_001699"
     CDS             515..934
                     /locus_tag="IWO38_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ0313788.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1026..1376)
                     /locus_tag="IWO38_001700"
     CDS             complement(1026..1376)
                     /locus_tag="IWO38_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313789.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1342..2673)
                     /locus_tag="IWO38_001701"
     CDS             complement(1342..2673)
                     /locus_tag="IWO38_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ0313790.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2929..3495
                     /locus_tag="IWO38_001702"
     CDS             2929..3495
                     /locus_tag="IWO38_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0313791.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3640..3727
                     /locus_tag="IWO38_001703"
     tRNA            3640..3727
                     /locus_tag="IWO38_001703"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3672..3674,aa:Ser,seq:cga)
     gene            4061..5035
                     /locus_tag="IWO38_001704"
     CDS             4061..5035
                     /locus_tag="IWO38_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ0313792.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5360..7255
                     /gene="asnB"
                     /locus_tag="IWO38_001705"
     CDS             5360..7255
                     /gene="asnB"
                     /locus_tag="IWO38_001705"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ0313793.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7450..7896)
                     /locus_tag="IWO38_001706"
     CDS             complement(7450..7896)
                     /locus_tag="IWO38_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ0313794.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8124..8273
                     /gene="rpmG"
                     /locus_tag="IWO38_001707"
     CDS             8124..8273
                     /gene="rpmG"
                     /locus_tag="IWO38_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0313795.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8361..8879
                     /locus_tag="IWO38_001708"
     CDS             8361..8879
                     /locus_tag="IWO38_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0313796.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8956..9912)
                     /locus_tag="IWO38_001709"
     CDS             complement(8956..9912)
                     /locus_tag="IWO38_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0313797.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10078..10884
                     /locus_tag="IWO38_001710"
     CDS             10078..10884
                     /locus_tag="IWO38_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0313798.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10881..11870
                     /locus_tag="IWO38_001711"
     CDS             10881..11870
                     /locus_tag="IWO38_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ0313799.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11867..12871
                     /locus_tag="IWO38_001712"
     CDS             11867..12871
                     /locus_tag="IWO38_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ0313800.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13003..13545
                     /locus_tag="IWO38_001713"
     CDS             13003..13545
                     /locus_tag="IWO38_001713"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ0313801.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13565..14263
                     /locus_tag="IWO38_001714"
     CDS             13565..14263
                     /locus_tag="IWO38_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ0313802.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14284..14496
                     /locus_tag="IWO38_001715"
     CDS             14284..14496
                     /locus_tag="IWO38_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ0313803.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14496..15458
                     /locus_tag="IWO38_001716"
     CDS             14496..15458
                     /locus_tag="IWO38_001716"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ0313804.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15476..15871
                     /locus_tag="IWO38_001717"
     CDS             15476..15871
                     /locus_tag="IWO38_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0313805.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16034..16399
                     /locus_tag="IWO38_001718"
     CDS             16034..16399
                     /locus_tag="IWO38_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ0313806.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16396..18207
                     /locus_tag="IWO38_001719"
     CDS             16396..18207
                     /locus_tag="IWO38_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ0313807.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18269..18436)
                     /locus_tag="IWO38_001720"
     CDS             complement(18269..18436)
                     /locus_tag="IWO38_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ0313808.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18684..19673
                     /gene="galE"
                     /locus_tag="IWO38_001721"
     CDS             18684..19673
                     /gene="galE"
                     /locus_tag="IWO38_001721"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ0313809.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19685..21169
                     /gene="galT"
                     /locus_tag="IWO38_001722"
     CDS             19685..21169
                     /gene="galT"
                     /locus_tag="IWO38_001722"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ0313810.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21193..22173
                     /locus_tag="IWO38_001723"
     CDS             21193..22173
                     /locus_tag="IWO38_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0313811.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            22262..23086
                     /gene="tsaB"
                     /locus_tag="IWO38_001724"
     CDS             22262..23086
                     /gene="tsaB"
                     /locus_tag="IWO38_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ0313812.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            23070..23612
                     /gene="rimI"
                     /locus_tag="IWO38_001725"
     CDS             23070..23612
                     /gene="rimI"
                     /locus_tag="IWO38_001725"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ0313813.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            23619..24083
                     /gene="rimI"
                     /locus_tag="IWO38_001726"
     CDS             23619..24083
                     /gene="rimI"
                     /locus_tag="IWO38_001726"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ0313814.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24080..25096
                     /gene="tsaD"
                     /locus_tag="IWO38_001727"
     CDS             24080..25096
                     /gene="tsaD"
                     /locus_tag="IWO38_001727"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ0313815.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
BASE COUNT         8327 a         4400 c         5377 g         7567 t
ORIGIN      
        1 aaaaaaagcg ttagcaacta tgcttaaaaa gcatatcgac tgaataaact aagcattgga
       61 caggctgaaa gactacattt aaaatgaaat gcgaagaaac aatagaaaat aaagttgcct
      121 tagaaccata gttgaatcac ctaacagtcg tgaagggaga ataagagaaa tggcagaaaa
      181 acaaaccgca ggacgagatc aattaggaga ttttgcacca aaatttgcag aattgaatga
      241 tgatgtttta tttggacaag tatggtccag agaagaatta tctgctcatc agcgtagtat
      301 catcaccata tctgcattaa tttcaggagg aaattttgaa caattgccag ctcatctgaa
      361 gattggcaag caaaatggca ttactagtga agaaatagca gaaatcatta cccatttagc
      421 tttttatgta ggatggccaa aagcttggtc agcttttagt ttagcaaaag aaatctattt
      481 agaagaaaat agcgaagata agaaagaggc gtaaatgacg acaaaacacg aagaaataca
      541 caatggcgtg atttttccag caggtgaaaa aaacgaagct tacgctcaag tatttaccgg
      601 gcaaagctat ctgaatatgt tagtgaatga tcccgaagtt tctgttggtg taggtaatgt
      661 aacttttgaa ccaggttgtc gcaatcactg gcatgtccat aaagacggct atcaaatctt
      721 acttgtgacc ggaggagaag gctggtatca ggaagaagga aagccagcaa gacgattaat
      781 ggctggagat gtcgtcgtaa cgcacaaagg gattaaacat tggcatggtg cgacaaaaga
      841 cagttggttt gaacatttgg cgatcactgc tggtaattca gagtttttag aacctgtaac
      901 agatgaagtg tatgaaggat tggaaaatca ataatgtcaa gcttgaacag ctcctgaaag
      961 tagattttaa tcgaactttc aggagctgct tttttgattc agtcttttat aaatagaaaa
     1021 tagggttaat aataggaatg agaagcacgt cgaaagatct taatattgat aatcggctgg
     1081 gcatacatga tttccacgtc atcaatcaaa ctacctaaca gatgataatc ttcccgatca
     1141 tgtcggggat tacctaatag ttcttgataa tagatttcca tactagggtc ccgaggattg
     1201 ttcagcaaat cagcaagtac atctaaatct tctggctgag tttctgccgt accctgtatc
     1261 tctatgaaaa gcccttcctc agcaaattct actccaatac gtatttcagt tatttcatgg
     1321 gaatataaat aagtaagaag ctcatcgatg attctttttg ataattcttt ttccaagtgt
     1381 ctttttttcc tttctgacag cggtaaagtt atctagtatc ggtacaagga tacctgcaac
     1441 gaaaccagtt gaaaaaccac tgttatatag atttagtcct ccgtgcaaag tgctgacgtt
     1501 atggacaaga gcaatatgta gtacaccagc tattacaccg taaaaactgc cgtaaaaacc
     1561 gcttatgggc gcgagacctg tcccaaacac agctgcaaca agcatgccag tactggtgat
     1621 ctcatgaaaa gttccaaaca aagaagcaat aaatattccg actagtatcg gaaaactgtt
     1681 ttttaaatga caaccaaaag cactgaaacc caccgcagat aaaatagcac cgatgacagg
     1741 tccatttaac tgaccgccaa ccagcaaaac ataagaagac agcatcagac caactaagcc
     1801 tatgttgatc aaagtagcac caataccaga attagcaatg aaatcagttg tcaattttcc
     1861 agatgaatcg aaaatttctc gaatccccaa aagccgaaag gaattataat agaaaccaat
     1921 tccgactgtc agcgaaaaaa agccaaaaat aatccaaatg agaggaaagt gatattcatt
     1981 gaaaaccaat gtattttcga cgattgaata gctgaataac ctcaaaacag cagtaaaagt
     2041 catcgcaatc aacccagagg tgaatccaat attataaagt gtgaatccac gatggaaaac
     2101 taaagtttga gcagctaagg gcggaagaag tagaccaatt gctatgccag ctaaattccc
     2161 caataaaata cctatcagta aaggaaaacg cataccaaaa gtgatataac taacgaccgg
     2221 tgataatgcg ctaccaaaca gtccgatcat gatgtactga gaaaaaggtt tattgacaaa
     2281 tacagcgtat agatacacac ctaaaatgat tgagatagaa ttataaaggt ttttgccaaa
     2341 aaaagagaaa ccggtaactg ttagaatcga agcaaataag ggaccgttta aaatcacctt
     2401 gtgccgataa gctaaaagca cagaaagtaa ggtcaaaatc cctacattta tgaatgcgct
     2461 tccgaaaccg cctaaagcaa aataatctgt caaaagattg cttggcgagg taagaatagt
     2521 tagcaatcca ctccaattat cagtaggagt attagaaagc aagccaataa aaagaaaaaa
     2581 aacacctgta aaaccaagat aaaaatactt tcttttctgt gaaacgattc gtttttgagg
     2641 atgaatataa ataacgtcag tttcgttttc cattacacac cttctttttc attttcttta
     2701 actataactt gaaatgaaag atttggacaa ctatcattta ggtcaaaact aaaaatgtaa
     2761 gggaacccat tgaatttaac caaatttttt taattttgca aaaaaaagtt aaagaagact
     2821 gaaaaaaaat ttaaaatata acgttttgag taaaatgttt gcaaaaaatg ctaaaaatag
     2881 ggtacaataa aacaacatat actgcagtaa agaagtgggg gagttttaat gagaagaaaa
     2941 gtctatacca aggatcaaat tctaaaagct gcttatgatg ttgttgcaaa ggaagggttt
     3001 aaaggattta ctgcgagaaa tattgctaaa aagatgggga tttcgacaca gccgatttat
     3061 ctggaattca aaaacatgga agatttaaaa aatacgttgc tggattctat ctttgaacaa
     3121 ctaaaagaag atgttttccc tattgaacgt actggtgata aaatcattga tctcggtttg
     3181 aactatatct actttgcaaa aaagcatcat agtctttaca tggcattgta cattgatgaa
     3241 catggggacg gaaagaaaat gcatgatttt tcatacaatt atttccgtga aatagttaaa
     3301 gaagatgaaa gatacaatca attatcggat gaatatgtcg atgctctgca tactggtaca
     3361 tgggtagcag tgactggctt agcgtcattg atgtcatcaa acatcatgca tccaacagaa
     3421 gaacaaatca ttgcactgat caaacggaca attgactcga ttttagcatt agataatcct
     3481 gaaataatca gatagtgttc tatcagtatg aatttgatcg gtaagtccgt tggctgcaaa
     3541 ctgtcaatgg acttttttgc taaaaaaagc gatttctgtt atttatctat tagaaatagg
     3601 cttgacattt cgtgaaaaaa gtttaaaaat agtacttacg gaaggttggc agagtggtaa
     3661 tgcaccggac tcgaaatccg gcgaaccggc taataccggc gcgcaggttc aaatcctgta
     3721 ccttccttat atcaacgatt agaggttgtg aagaagctgt cctgcatcaa gtaataagaa
     3781 cggccaaaca aaaatagctt ttcctatttt ttcgtttgat tgggcttatt accgcagatg
     3841 caagcttttg aacaccgttt gttcggaata aggggctgtg acaagacttt tgtcacagcc
     3901 ccttattgtt gtacttttaa cctacaatat tttttgattc cccctgagta ctatttttta
     3961 taaatgctag taaatcaatc ttctttggcc tttttttgat aaaaaaaata ctagatgtaa
     4021 gataaaagtg taaaaacgat ttgttttagg acgggaaacg atgtgtaccg caatcactta
     4081 tgcaacgaaa gaccattatt ttggaaggaa ttttgattat gagatgtctt ataatgaagt
     4141 ggtcactatt actccaagaa attatcgttt cgattttaga aaggtaaaaa atttagataa
     4201 acattatgca atgataggaa tagcagctgg ggtatccaac tatccactct attatgaggc
     4261 tacaaatgaa aaagggttga gtatggcagg actgaatttt cctggcaatg ctgattataa
     4321 agaattacaa gaaggaaaag ataacgttgc accatttgag tttattccat ggatactagg
     4381 gcaatgctca accatagacg aagctaaaga attattggct actatcaatc tagtcaatat
     4441 cgattttagt gaaaaactac ccttgtcacc tttacactgg ttattagctg ataaagaaaa
     4501 atcgatagta attgaaagta tgaaagatgg acttcattta tatgataatc ctgtcggtgt
     4561 gttgacaaat aatccgccat ttgattatca attatttaat ctgaacaatt atcgctcgct
     4621 atcaaatgga acaccagaaa atcatttttc aaaccagatc agcttgaatg tttatagtcg
     4681 tgggatggga ggattgggct tacctggaga tctctcttct gtttctcgat ttgtaaaagc
     4741 cacatttact aaaatgaatg ctgcatcagg tgattcagaa tctgaaagta tcagtcaatt
     4801 ttttcatata ctaggctcag tcgagcaaca aaaaggagta tgcgatactg gagaaagaaa
     4861 atacgaatat acgatttatt cttcttgctg taacgttgat aaagggattt attattatcg
     4921 tacgtatgaa gacagtcaaa ttactgctgt aaatatgaat aaagaagact tggatcgtca
     4981 tgaattggtc agttatccag tcataaaaga gcaacagata aactatgtca attaattaat
     5041 gaatagatcc gtttggtatc atattttctg atacagtcat aattagacac gatctttagg
     5101 tcgtgtcttt ttttgctggt ttttttggaa atagcatcca ctaatatttt tttaacacga
     5161 agagaataag gtagcctcaa aaaagaaaac atggtaaatt agatattggg actttatttt
     5221 ctgtaaaaac agaaactttc atcttttgtt ttcacgtgac gaaattgtaa gagagaaaat
     5281 attcgtccgc tatttattct atgataagat gcatatgaat gaaataaaca gtaattttat
     5341 attaggatgt gaaagttaaa tgtgcggtat tgtaggtttt gttaacgaca aggacaacaa
     5401 aaaaacaatt attaatacaa tgatggatcg aatcgttcac cgtggaccta atagttcagg
     5461 agaatatatc gataaacatg ttgctttagg attcagaaga ttaagtatta ttgacttaga
     5521 gggcggtact cagcctattt acaatgaaga tcggacaaag atcattattt ttaatggcga
     5581 aatctataat taccagcctt tgagagaaga attgattgca gctggccacg tttttcaaac
     5641 acatgcagat acagaagtcc ttttgcatgg ttacgaagaa tggggaacag aattattgca
     5701 aaagatccgc ggaatgtttg cttttgctat ttgggataat gaaaagaatg agttattcgg
     5761 tgcaagagac cactttggga tcaaaccata ttattatgca gaaatgaacg gaacatttat
     5821 gtttggttca gaaatcaaaa gttttctgcc tcatcctgat tttaacaaag aattgaacaa
     5881 agaagcgttg aaaccttata tgactttcca atattctcca ttaaacggcg aaacattctt
     5941 caaagatgtc tatcgtttgc ctgaaggcca ttattatacg tataaagatg gaaaaataga
     6001 cattcagcaa tattgggatg cagattttga aacaaaagaa acgcattccc gacaagaatg
     6061 gatcgaaaaa atcgacgaaa ccgttcaagc ttctattgaa gcacatacgg tcagtgatgt
     6121 agaagttggt tctttcttat ccagcggggt tgattccagt tatgtaacct ctgtattgaa
     6181 accagatcat tctttctcaa tcgggtttga tgataaaacc tataatgaag cgattgaagc
     6241 cagaaaacta accgaactat tggacttaga taatacagca gcagtgatcg acggagacat
     6301 gtcgttcaaa gcattccctt tgatccagta tcacttagat gaacctgatt caaatccttc
     6361 ttgcgtacct ttgtactttt tagcaaatct tgcttcacaa agtgtgcggg tagtccaatc
     6421 tggagaaggg gcggatgaac tgtttgcggg ataccaaacg tatggattcc atacgaattc
     6481 aaaattcatc cgtgtgattg ctcaaggatt gaaaaaatta ccaaaaggaa cacgctataa
     6541 tttaggacgt aaaatcggaa aaatgaaaaa tttccatgga cggatccatt tatatgaatc
     6601 acttgctcct gcaaaagaat attttatcgg tcatgctcga gtgttcgaag aatcagaagc
     6661 atcagaagtg ttaacaccaa aatatcaaac agcaccatct gtggatgaaa tcatgactgt
     6721 tcattacgag aaaacagaag gaatcaaaga tgaagtcaat aagatgcaat atgtcgatct
     6781 tcatcaatgg atgccaaaag atatcttgtt aaaggcagat aaactttcta tggcaagttc
     6841 tttggaagtc cgagtacctt tattggatat cgaagtcatg aaattggctc aacaaatacc
     6901 aagtaagttt ttattgaatc aaaacaacac aaaagatatt ttcagacaag ctgcgaacaa
     6961 acatcttccg gaagaatggt cgaatcgtgt gaaattagga ttccctgtgc caatcaaagc
     7021 ttggttgaaa gaagaacacg gctacgaaca agtcaaagca ttattcgaag cagattttgc
     7081 aaaagaattt tttgatcaag aaaaaatcat gaagttgcta gatgaccatc acgaaggacg
     7141 taaagaagag caaagaaaaa tctggacaat cttcagtttc ttgacatggt ataaagtcta
     7201 ctttgtagat gaaacgattc cgcaagcaga agcaatcgac tatgtgactg tttgatgaaa
     7261 atcaaatatc aataaaaaag aggttttctc ctaccgttta acactgagtg ataagaaaca
     7321 atttcaaaaa acagctcttc ccgttttata gatattatga cttatggttc ggagttggca
     7381 gaggaacacc attgtttcga acataagggg gttgtgacac gattttcgtc acaacccctt
     7441 tacatatgtt caataatcga tttctttttc ttttgtccca tcttttttat aaatgaacaa
     7501 agttgattct ttgttttgtg ctacttgctt tcctttttcg attgcttctt tttttgtatc
     7561 aaaatggttg cttgcctttt ttgccccttt agatttcacg atccattgtt cttcctcata
     7621 ttctacgatc acatctgaat caagcagttt tccagcacga ggattcgtat cgtgttcatc
     7681 cgtcttgctt ggattttttt ctttttcgaa agttcgtttt tctgattcac tagcatcttc
     7741 ataccatttt tttgcttgat caatggcaat tgggatcgca cggttatcag ggtacccttc
     7801 atctaataat gcatttgcga tgtcgattgc tttttttctt gtcaatttat ctaagttttt
     7861 cattgatgca ggaaagtcct tcatgttcca tggcatatta gtatccccct ttattaaaaa
     7921 acataatcta tataaacaag tgtaagggta tttatacaaa aagaaaaaga ttttgtttga
     7981 gaagtaaaac cgttgtattt atcgtcaagt agttttactt aacaaaatcg tgaaaaacac
     8041 tagcaatcta gaaaaaatca tggtaagata atcaagtctg agagtcatct tagaggattc
     8101 ttgtaaaaat gggagggaaa aacatgcgcg taaacatcac tttagagtgt acttcttgta
     8161 aagaacgtaa ctatctaaca agcaaaaaca aacgtaacaa tcctgatcgt ttggaaaaac
     8221 aaaaatattg cccacgcgaa cgcaaagtta ctttgcaccg tgaaacaaaa taatgttttt
     8281 aaagttctca attccttgct ggtcaaagaa ttgagacttt ttttatctca aaacatctaa
     8341 aatcataagg agcggataat atgtttattt ccatgtgggc acaagataaa aatggtctga
     8401 tcggcaaaaa tggtttgtta ccatggcgat tgccaaatga tatgcgtttc tttcgcgaac
     8461 acacaatgga tcgaatttta gtgatgggaa gaaagaccta tgaaggaatg ggggatcttt
     8521 cacttcctta tcgccatatc atcgtgttga caacgcaacc aaattttcgc acgaaagaaa
     8581 atgcggaagt aatgcattcg atcgatgaac ttttgtcgta tagtcaaacg atctcagaag
     8641 atatctatgt ttcgggtggt agtcggattt ttcaagaact tcttccttac actggtcgga
     8701 tctggcgaac attgatcgat agcacgtttg aaggcgatac gtatattggg aatattgatt
     8761 tttcagactt cgcccttata gaggagtatg aggggataac ggatcaagaa aatctatatg
     8821 cccatcgttt tcaaaaatgg gaaagagtaa aaaatagggt tgcttcaaaa gaagaataaa
     8881 atctatgcgt ctagtttgtt aataagaaaa aaagcatctc acgactaatc gtgaagatgc
     8941 tttttttagt taaaatcaat ttagtttttc tttgatatca ttgaccattt cctgactggc
     9001 aataggtccg ttatatagcc aactagattc aggtccgaat tgcagtacat gattgtctgt
     9061 cactgctttt agattcttcc agcttggttc attgaacatg gcagcatctt tgtcactgtt
     9121 cacaaggatg atgtagtcag catctaattc agctagcttt tcagaggaaa cagcagacca
     9181 gtcagaagtg gcttttttgg agacctcttc cactagatca ggaacaccaa attccagatc
     9241 gtcgtatacg attcttccac ttgaccgatt tttgctgacc ataaaggctg agttgttggt
     9301 cacccacaac actgcagctg tcttgttctc gattttatct gctagttctt ctcgagtttt
     9361 cttgatggat ttttgataat cggaaatgat tttttctgcc tctttttctt ttgccaaagc
     9421 tttgccgaca tcttttagtt gttcttccca tgtgacatct gttccgtttt ttacaacata
     9481 agttggcgcg attttgctat attgttcgta tttcccgcct tcgacagtag cggaagaaga
     9541 aatcaacagc aaatcaggct caaagcttaa cactttctca taaggaaggt cataagaaat
     9601 cgttgggaca tcctttaaat catcttgcag ataatcttga atggaaccac tgccaacagt
     9661 ccactgggcg ataggttttt cccctagtgc aatcagatag tcttctaagt aactgccgat
     9721 gatccgtttg ggcgcgtttg gaatctctac tttatgtcca agtgtatctg tgactgtatg
     9781 agtactattg gatgaagtcg tcgaattggc agttgtagct gtttctttat ctgaagaggg
     9841 ggagcaagcg ctcagtactc cagccagcag caatatgcta gataatgtga atacttttgt
     9901 ttttaatttc atttctttcc tcctaacata attgagaatc tttctcattg gttattattc
     9961 ctgatatttc gaacgctgtc aaccgcttca tttggctata gatatgaaag tagcgcttga
    10021 cgaaatgttg aaacagcgtc ataattgaga atgattttca gcgaaaggat gggtgaaatg
    10081 acgagattaa gagcagctga cattgcagcc ggctacgaaa acaaaaaaat cattgaaaac
    10141 atgtctgtta cgattcctga agggaaaatc actagtttga ttgggccaaa tggaagtgga
    10201 aaatctactt tattgaaaag ttttacccgt atactacctg tgaaatcagg aaagatcatt
    10261 gtcaataact ctgatatctc tacatacagt tcaaaagaac ttgcaaaaaa aattgcatta
    10321 ttagcacaaa ctagtgaaca ccctacaggt atgacagtag aagaagtagt atcttacgga
    10381 cgataccctt atcaaaaact gttcagtggc atggacacgc aagatcatga agcaattgag
    10441 tgggcgttgg atgctacagg tctagctgga tttcgtatgc gggatatcgc aacgctgtct
    10501 ggtggacaaa cccagcgtgt ctggatcgct atggcgcttg ctcaggaaac agatatcttg
    10561 atcttggatg aaccgacaac atttttagat ccggcacacc aattagagat cctacagtta
    10621 ttgactgaaa tcaatcaaat gaaacaaaca acgatcttga tgtcgattca tgacattaat
    10681 catgcttcac gtttttcaga ttatttatta gcaatgaaaa acggtaagtt gatcacagaa
    10741 ggtacacctg aagaagtgat cacaaaagag tgtatgaaaa aaatatttga gattgatgca
    10801 acgattgtca cgcttccaag aacaaataaa ccagtttttc taacctatga tttgttgcca
    10861 aaagagcagg aggatcaatt atgaggacta tgaagaaaaa tagactcctg cttttggtct
    10921 gttttgttct tttgattatt ttttttgttg ctctttcaaa tggcgcagcg actgtgggaa
    10981 aagcagattt ttggcaggcg ttgtttgatt ttgatagatc aaaacaaagt caccaaatca
    11041 tacgaaatat tcgtttgcct cgtgtggcgg gagcatttct tgtagggagt tgttttgcat
    11101 tgagcggtgc attgatgcaa ggagtgacaa gaaatccttt agcagattca ggcttactag
    11161 gaatcaacgc gggtgcttcg ttggcaatgg cccttagttt tgctttttgg cctaaagctt
    11221 ctgatttttc tgtgtttcta atgtcattgg caggttcttt tgctgtaact ttgctcgtat
    11281 ttggtttttt gcagttttcg aaaatgggca tgaatccttt acagctgatt ttagctggcg
    11341 tagctattag ttcttttttt actgctatca gccaagcact tacccaattc tttaatctgc
    11401 agcaggatct tgctttttgg tttgtaggcg gagctgccaa tatcacatgg aaacaattag
    11461 cagtattgtt tcctttatat atacttgctg ttttggtttc tttttcatta ggaaaagcaa
    11521 tatctttgat tcatttgagt gaaacacatg cattagctct tggaagtcat cctaaacgaa
    11581 cgcggttgat tgttttctgt ctagttgcag tgttagcagc cattgccgtt tcgttagtcg
    11641 gacctgtttc atttattggg ctgttggttc cgccagttgt tcgcggaatg gttggaacgg
    11701 actatcgtta tgttctacct gtctcttttt tagttggcgg tggattggtt atgttggcgg
    11761 attttacagc gaggatgatc aatccccctt ttgaaacacc ttttggatta gtcattgcat
    11821 tgattggcgt tccgttttta ctgatacaag taaggaggga acaacggtga aaaaaatata
    11881 ttggacgttc tatttcttgc tttttgtact tgtcatcctg tctataggta gtttaatgat
    11941 tggtacgcct tttatttacc tggatcagct ttttgccacg ttcataggga aagaatcatc
    12001 ttctttggcg ctgattgtta gagaatttcg agttcctcga ttactgatca gtttattagc
    12061 aggagcttgt cttggcgttt ctggttatat cttgcaagga gtaacaagaa atgaattggc
    12121 agattctagt atattgggca ttaatgccgg agcaggattt ttagtgatgc tttatttagg
    12181 tttcttttcg cactattcgc tgccgtttct attgctagtt gtagcctttc taggtggtgt
    12241 tttagctgct tgctgtgtct atcttgcggc ctatgatcga aatggctttt tgggtatgaa
    12301 tcgaatctta ttgtccggta ttgccgttaa tgccggcctt tctgcattaa cattgctatg
    12361 cacgatccaa ttatctaagg aaaattatgg atttgtgaat gcatggttag cgggatcaat
    12421 ctggggtgcg agctggcctt atgtttgggc attgcttcca tgggcggtta tcttaattcc
    12481 gctaggagga ctttatgcac agaggattgt attgctatca tttggtcaag aacgcgcgca
    12541 aggattaggc cttaacgtgg ctaaaagtca aaaaatcctt ttgttgttag ctgttgcttt
    12601 ggcaagcggt agtgtagcgg tgacaggtag tttatctttt gtaggactac tagctcctca
    12661 tgctgcgaag ctagtcgtac gtaaagaaaa taactggacc tttgttctga gcggtttgtt
    12721 cggttccatt tttctgctta gtgcagatat ggttgctaga gtgattctac taagtgggga
    12781 agtacctaca ggaattttga ttgccctttt tggtgcgcca tattttctgt atgcactttt
    12841 tatcagacaa aaacacgcgc tgaacagttg aaattcatta aaaaaggaag gccaatcatt
    12901 tctaggtctt ctttttattc tatttatgca tttatttgaa agttcgattg ctatttttct
    12961 ttttttccta taccatttta tgagagaagg aagggtgagt ttttggacca attgaaagta
    13021 agattacgcc ggaaaggaat cgaaaagctg aaaaatctgg ccaaaagtgg caagaaaaag
    13081 gaaaaagaac agcgaatcta taaacagcta ttcgccagcg agtactggaa gtcagcaaaa
    13141 gtgataggga cgactttatc gaatgaattt gaactggata cacagccggt tatccaacga
    13201 gcagaaaaag aacaaaagaa aatagttgta cctaaaactt tgccgaaacg tcagatggcg
    13261 ttttatgaaa tggatgaaca gtccgttctt gtacgttcga gttttggtgt tcttgagcca
    13321 caatcagaga agctctattt gccggagatg atcgatctcc tcattgtacc aggtattatc
    13381 tacaatagag ccggttatcg aatcggtttt ggcggtggtt attacgaccg gtatttaaat
    13441 aattttcata aaaaaacatg tagccttgtt tttcgtgaac aattgaatga acaatggcaa
    13501 ccggagagct ttgatcagaa gatccaacaa ttgtttattg actaaaatga aatgggaggt
    13561 tttcatgaat tatcaacagc aaataaaatt gcgtgcatgg ttaagacgac cgttatggac
    13621 ctatgctttt ttagggatac agacagcggt cttcctcatg atggaattat ttccgcgact
    13681 tgagatccct tattatgccg gaatgtatgg tccttatctt gttcacttca atgaatggtg
    13741 gcgtttagtg acaccaatct tcatccattt tggactgatg catttcgtaa tgaactcact
    13801 gatactgtat tttatggggc agcaaatcga ggcaatctat ggccattggc gttttttctt
    13861 gatttatatg ttcagcggga taatgggaaa tacagcaagc tttgctttta acgaagcgaa
    13921 tgttctgtct ggtggtgcaa gtacttcgat attcggtttg ttcggtgcgt tgtttatctt
    13981 agggtttcat tttaaacata acccagcgat ccagcagcta gtcagacatt ttcttttatt
    14041 tattgtgttg acatttgttt ttggtcttct ggatacctct gtagatgttt ggggacatgt
    14101 cggtgggcta attggcggat tagtgttagg gaatgttttg ggtctaccga aacaacagac
    14161 aagctattcg atacaccaaa ggattctttc tacactagtt tttgtatttc tttttgtaat
    14221 atgtatttta ctgggtttaa aaaaatatgg tttacttgta taatatgaag gaagtgctta
    14281 aagatggaga gcttatatga tgtacagcag ctgttcaaaa aatttggcat ctttatctat
    14341 gtgggtgcac gcatctatga cattgaatta atgactatcg aattgcgtaa tctgtatgat
    14401 agccgtttga ttgatcatga tacatacatg acagcgtggc gcatattaaa aagagagcac
    14461 cgaattgaag aaagccgaaa gaaaggaaat ttgtaatgga caagaagatt attgggattg
    14521 atttaggtgg aacaacagca aaatttgcaa ttttaacacc agaaggagag attcagcaaa
    14581 aatggagcat cgataccaat attttggatg atggtaagca tatcgttcct gaaatcattg
    14641 aatcaatcaa tcatcgtttg aatttatata atatgaaagc cgaagacttt atcggaatcg
    14701 gcatgggtac cccaggaagc gtggacagcg aagctggaac ggttattggt gcgtataact
    14761 taaactggac agaagttcaa tttgtgaaaa aactgatcga ggcaggaaca ggaatcaaat
    14821 tcgctatcga taatgacgcg aatgttgctg ctttaggtga acgatggaaa ggtgctggag
    14881 aaaacgatcc agacgttgta ttcgttactc taggaacagg tgttggcgga ggtatcgttg
    14941 ctggcggaaa tttgattcac ggcgtgagcg gagctggagg agaaatcgga catatcactg
    15001 tggatcctga agggttcgaa tgtacttgcg gtaaacgcgg atgtttagaa actgtatcta
    15061 gcgctacagg tgtggtacgc ctaggtcgtt tcttggcaga agaatacgca ggggattcaa
    15121 aactgaaagc aatgctagac aacggagaag aagtaacaag taaagatatc tttgaaatgg
    15181 ctcaagaaga cgatccgttt gcattgatgg ttgttgatcg cgtatgcttc tacttaggtt
    15241 tagcttgcgg aaacttgggg aatacattaa atccgtcaag tatcgtgtta ggtggaggag
    15301 tatcggcagc aggagaattt ttacgtagcc gtgtagagaa atattttaaa gaatttactt
    15361 tcccgcaagt aagagaatca acaaaaatca aattagcaga gcttggtaat gaagctggtg
    15421 tcattggtgc tgcctcatta gccttgaaat ttgcttaaaa aaacggaggc attacatggt
    15481 tttatgggta attaatatca ttttgctatt gattgtgctt gcaatcatcc tttgggaagt
    15541 ctatctacga gtaatggcca aacgttcagc aacgactttg acagaagaag aatttcgaga
    15601 aggaatgaga aaagcacaag taatcgatgt tcgcgaaaaa gatgtgtttg atgctgggca
    15661 tattttaggt gcaagaaaca tcccttacac tgttttgaaa gattcattag gatctattcg
    15721 aaaagaccag cctgtctata tttacgatca aaagaaaagt ttgagcatcc gtacagcaaa
    15781 taaattacgt aaagcaggtt atcaagagat ttatttactt aagggtggat atgacggatg
    15841 gtctggaaaa atcaaaagta aaaaagtata aaacagatga ttgtatgaca gctttaaaaa
    15901 ccgaatcaat cattagattt tgaccaagat cacatgaacg gcgcaatgga tagttgtgcc
    15961 gttcattttt tttattttgt gctattatct acatatataa gctaagcgaa aaggtagaaa
    16021 gaaggaaaca actatgatca aattaaaacg tgtttacctt gatgctgaaa aagaggatgg
    16081 ctatagaata ctcgtggata gattctggcc acgaggaatt tcaaaagaaa aagcgcagtt
    16141 agatctgtgg ttgaaagaaa ttgcacctag taatgaagca cgcaaaactt ttgaccatgt
    16201 accagaacga tttgcttcat ttgcaaagaa ataccagcaa gaattgattt tcgaagaaga
    16261 aaagaagcaa gctattttta aactcagtca acttatgaaa gaacatccag tcattacttt
    16321 tgtttatggt gcaaaaaatg aacatcagaa ccatgctgtt ttattgaaaa aatttatcga
    16381 aatggaggca tgcaaatgaa aatagggatc attggtgccg gacctagagg attaagtgta
    16441 gcagaaagga ttctccataa taatcaggac agacattctc ttgaactttt tcttttcgat
    16501 cccaatggtc ctggaggtcg agtttggcgg ttgaatcagc caccggagtt attaatgaat
    16561 tctgtttctc aacaagtgac gctttttaca gatgaaacat tgtccagtgg aggattggta
    16621 acacagggac ccaacttgtt tcaatggagt aaaggacctg ctacagatta tatcgaacag
    16681 acggtaacca aaaataagca actaattata gaagaaatca atcgtctgga aaaagatcag
    16741 caatctactc gatgtttata cggactctat caacgctggt ttttctcaaa gttgcaagaa
    16801 gaatttccag cttctattca gtttgtttat tctttagtaa aaaaagtaag caaaagaaaa
    16861 gatggttatc ttttacagac agatcagcaa agctattcta tcgatcagct tgttttagca
    16921 agcggtcact gggaaaatga tgagacagaa gaagaaagag tgtgggtaga ctacgcaaag
    16981 aaaaatgttt tactgtatca gcttccttct aatcctgcag atgtagatct ttcgaagata
    17041 ccgcctaaag aaaatgtctt tttaagaggt ctaggcttaa gtttttttga ttatgtcgga
    17101 ttgtttacag tatcaagagg agggcgcttc gagaacgaaa agggaaaact ggtctatcat
    17161 ccatcaggca aggaaccgat tgtatacagt ggatcaagaa gggggctccc ttattaccct
    17221 cgaggaagaa atcaaaagca aggcggagca atggcatggc cacggttact aacaaaagaa
    17281 aacctggagc aatggcatca gtcgggattt ttgactggcg aaacattttt tgactatctg
    17341 aaaaaagacg ttgagttgtt ttattacaag aaaatcatcg aagaatatca cttgccgatt
    17401 ccaaagaaaa attttgagca tgattttctc tccctctctt cagaggagtg tctcggaaag
    17461 catccagcac taatccctta tagatgggat tggtcgtatt tagaaactcc tcttgcctat
    17521 cagaatgagc ccttttcaga agctagccga atatttattg aagggcagat aaaagaagcg
    17581 gaaaaaggca attgcacagg agcatttgct tcaacatttg atgcattaaa agactggcgt
    17641 gatccaatcc ggcaggctat tgaatggcac gttttttctg ctaaggaata caaagaaata
    17701 ctatggggat ggtttactca tctaaatagc tttttaacga tcggtccgcc aatcattcgg
    17761 acgaaagaat tagcagcact gatagatgcg ggtatttttc atttgatcga accgccattt
    17821 gatattcagc aaaagcacaa ccgctatgtt atttctctac aagataaaga aatcagcagt
    17881 cgctatttga ttgaagctcg attgccacaa acaagctttt ctcgttcaag taatcctgtc
    17941 ataaaaaatt tgaaggaaaa caaacttgtt cgcccttttt cattttcaga taatacagga
    18001 agttatcatt cagaagctat agatgttgag cgaggtacga atcgcgtgat tgatgcttca
    18061 ggggaaatcc agtctgactt ttattgctat ggaatacctg ttgaaggtgt ggattggcta
    18121 actgcagcag tagctagacc ttatacagat ccatggaatc tgagacaggc agataaaata
    18181 gcacaactta tattgactga caaataaaaa tacgcatgat gacttatctg ttttttagaa
    18241 agtcttcatg cgtattttta aataattatt aacgagaaac gcgtttacgc attgcttttt
    18301 tacggttttc ttcgatcatt gcttcttttt cgtcaattgg atcgatcact gtttttttca
    18361 cagaagcaac caaaccagca acggcagcgg ctgttgccag acttcctact aatattccag
    18421 atacaaattt tttcattgaa tccacctcat ttattatttg ttatatctat tatggaacaa
    18481 aagagaaaaa aaggctagtt ttacgtttgt ttgtaaacct ttgcaaaaaa tccttgaaaa
    18541 tgtttactaa atgttttatt ataaatttac catatgaatt tacgaaattt tactttgttt
    18601 gttttcacca taaaagtaaa cttcgttttt taaattatat agaatcaaca aacacaattt
    18661 tactaatcaa aggagtgttt tttatgacaa ttttagtact aggcggagcc ggttatatcg
    18721 gttcgcacgc tgtagaccag ctagttcaaa aggggtatca agtagctgtg gtagataatt
    18781 tgctcacagg tcacaaacaa gcagtacatc cagatgctca tttttatgaa ggagacatcc
    18841 gggataaaga atttctacgt tccgtatttg aaaaggaacc gattgaagga gtaatccatt
    18901 ttgctgcaag ctctttagta ggagaatctg tcgaaaaacc attgatgtat ttcaacaaca
    18961 acgtatacgg tatgcaaatc ttacttgaag tcatgcacga gttcaacgtg aataaaatcg
    19021 tattctcttc tactgcagca acttatggag agccgaaaga gtctccaatc acagaagata
    19081 caccagctaa tcctaaaaat ccgtatggtg aaagtaaatt gatgatggaa aaaatgatga
    19141 agtggtgtga ccaagcatat ggcatgcgct atgtcgcttt gcgctacttc aatgtagccg
    19201 gagcaaaagc agatgcatct atcggagaag atcatacacc agaaacgcat cttgtcccaa
    19261 tcattttaca agtagctctt ggtcaaagaa aagccttggc tgtttatgga gacgattatg
    19321 atacaccaga tggaacatgt atcagagatt atgttcaagt agaagatttg attgctgcac
    19381 atattttagc tttggaatac ttgaaagaag gaaatgaaag caacttcttc aatttaggca
    19441 gcagcaaagg atactcagtc aaggaaatgc tagaagcagc tagagaagtg acaggaaaag
    19501 aaattccagc agaaatagca ccaagaagag ctggagatcc tagccgatta gtcgcttcaa
    19561 gtgaaaaagc gagagagatt ctgggctgga aacctgaata cacagatatc aaagcgatta
    19621 tcaaaacagc atgggattgg catgtcagcc atccaaatgg ttatgaagaa taggggaatg
    19681 agatatgtca atcagtcaaa cgatttgtga ttttgccaca cttgcgatcg aagctggcgg
    19741 atggatggaa atggaccgct tatatttaca gaatcgtctt ttagggatgg taggagaaga
    19801 tgctctagaa agcattgaag taagaccagt taaggaagaa tctgtagact tattggatca
    19861 gcttgtcgct aaggcaagag aaaatggtgt tctttcagaa ttgccagcag aacaagagat
    19921 ttttgaagcg caattaatgg attttctcac accgccacct tcagtcgtca atgcattctt
    19981 tgcacagcac tattcgaaaa gcccagagga agcaactgag tatttcttta atttaagtaa
    20041 gaaaaatgac tacatcaaaa caagagccat cgcaaaaaac atcaaatttc cggcagagtc
    20101 ggagtatggt gaactggaaa tcacaatcaa tttatctaag ccagaaaaag atccaaaaca
    20161 aatcgcagca gaacgcgagg cggtaaaagt agactatcct acctgcatgc tttgcatgga
    20221 aaacgaagga tatagaggtc gcttgaacta tccagcccgc accaatcacc ggattattcg
    20281 aatgaatcta gacggtgaaa gctggggatt ccaatattcc ccttatgctt attacaatga
    20341 acattcgatc attttatcag aacaacaccg cccgatgaaa atcacaaagg aaacatttgc
    20401 tagactgctt cggattactg aagtcttgcc tcattacttt gttggttcga atgctgactt
    20461 accaattgtt ggaggatcga ttctttctca tgatcattat caggcaggac gtcatgaatt
    20521 tcctatggca aaagcaccga tagaaaaact tatcaaacta gaagattatc cgcaagtcat
    20581 cgcaggagtc gtgaaatggc cgatgtctgt gatccgtttg caatcaacag agaaaaactt
    20641 gcttattgaa gcagctaact cagtattgga aaaatggaaa gcatattcgg atgaaacagt
    20701 atctatcaga gctttttcag cagatggtac accacaccat acgattacgc caattgcacg
    20761 aagaagggac aattactttg agttggacct tgtccttcga gacaacaatg tttcagaaga
    20821 acatcctgat gggatttttc atccacataa agatgtccaa catatcaaaa aggagaacat
    20881 tgggttgatc gaagtaatgg ggcttgctgt tttaccacct cgtttgaaac cagaactagt
    20941 agaagtagag catttcttat taggagaaga aaaccagatg gctgaatatc atcgtccgtg
    21001 ggcagaacaa ttaaaaaaag aacatcctga tttgacgaaa gatacggtca ccgatgtcgt
    21061 acaaaaatct gtaggtcagg tttttgcacg tgtactggaa gacgctggag tattcaagcg
    21121 agatgaaaaa ggacaggcag cgtttgaacg atttactgcg attttataaa cgtaaaaaag
    21181 gaggagagac aaatggcaac aattaaagac attgcgcagt tggcaggagt ttctcctgct
    21241 actgtttcaa gagttctcaa ctatgatcct gatctttccg ttggcttagc cacaaagcaa
    21301 aaaatttttg aagcagcaga acgcttaaat tatacgaaac atcgtaaaaa tgcagcaaat
    21361 actaagcaaa cgcttcgcct gatccagtgg tacgacgata cggaagagct tgaagatctt
    21421 tattatttat ctattcgcct aggaatcgaa aaaaaggcag aagaagcaaa cgttacttta
    21481 ttgaaagaaa cgtgggattc cattagtgat cttccggcag atggaacgat cgcactagga
    21541 aaattcgacg aagctcagtt attagaaatg aaaggatccc aaaatgcctt gcttttggta
    21601 gattctgacg gcacttctca aggaatcgac tctctagttg tcgactttaa gtcaagtgtt
    21661 caaaaagtca ttgaacactt tgtctcccat aaagcgcaac atatcgctat gcttgctgga
    21721 acagaatata cgaagaaaaa ccatcagcgg ctaaaagatc ccagaattat ggcatttcaa
    21781 caagttttgg cagaaaaagg ctacgacgaa ggtccaatta tcgaagcaga tttttctgtc
    21841 gagtcaggat atcaagcagt gaagcagtat ttaaaaacga atccgcatgt tcctgatgcg
    21901 ctttttgcag ctaatgatgc attagcgatt ggcgcactga aagcgattca agaaaaaggt
    21961 ttagtggtac cagaagatat ttctgtgatg ggcttcaatg atgtcagtgt agcgaaatat
    22021 gtaacgccct cgctttctac tgtaaaagtt tataccgaat ggatgggaga gctagcagtg
    22081 gagacgattc ttcaattaag taattccaaa gcaccggttt caagaaagat tattgtggaa
    22141 acagagttga ttattcgaga atcaacgaaa taaaagatgg aaaagctgtg ggaaaatgga
    22201 gtgataccgc agcttttttg tgtttcagga aggggcaaaa tttgcccgcc agcagataat
    22261 catgtattat tgtactagtt ttgcctttgg tattttcatc ctcagaataa aaaagtatgc
    22321 tacaatggaa ggcgagaatg tcatagcaga aaggatcaaa tatatgatta cattaggaat
    22381 agatactgca aaccaaactt tggcgatcgg tgtggtagaa gacgaacaaa ttcttggaca
    22441 aatacaaaca aatatcaaac gcaaccacag tgtaacactg atgcctgcaa tcgaccaatt
    22501 gtttgcagat cttcaaataa gtccaaaaga cattgaccga atcgcggttt ctgatggtcc
    22561 cggttcttat acagggcttc gtataggtgt aacgacagca aaaacgattg cttatacatt
    22621 agataaagag ctagtaggag tatctagtct gaaaaccatc gcggctaatt gcatcggtgt
    22681 ggataatatt attgtgccca tgtttgatgc tcgaagaaaa aatgtatacg ctggggcata
    22741 ccgctggaaa gaaaatagat tagaaactgt acttgaagat gtacatattt ctgcttctga
    22801 tttgttcaca caactacaaa agctgaatga agctatttat ttcgtcggga gtgattgtca
    22861 caaattcttc tctgaaataa aggaaatatt gcctagtgcg atgattaata cagttccagc
    22921 atgggatatt ccaagtggtg taactgtggc tcaattaggt gctttagttg aaccagttgc
    22981 agagaaacaa gcttttcttc ctcgctattt aaaacgagta gaagcagaag aaaaatggtt
    23041 ggagacgcac acacctggag acgaaagtta tgttgaaaaa atttagagcg tatgccagaa
    23101 gcttttttca agataaaatg atttatacag aaaaaacagt cgaacaaatc gatcgtcgtt
    23161 acgtactaag agaattgact acagaagata tcaaagagct gctggatgtc gaacgagaag
    23221 tctataatgg cgaattgcca tggacaaaaa gtgcctttat atcagaattg aagtcaccgc
    23281 ttcctcatct atatctaggt atctttgata atggaagaat gattggtttt atcggttcaa
    23341 gagcgttcgg tttagattgc catatcacga atattgccgt tcgaccggca tatcagcgta
    23401 aacgaatcgg ttcgctgctt attgacgaaa tcgagaactt tgctataatg aatcgctgtg
    23461 agacgatgtc attagaagtg cgtatgagta atcaagatgc acaaagactt tatcggaaat
    23521 tggggtttca agcaacaacg atcaaaaaaa gatattacac tgaaacaaaa gaagatgcgt
    23581 tggatatgat aaaactttta gaaaatgagt gagattagtt gatttgcaga aaaaaagatt
    23641 ttgatgaagc gtacttggca gaatgcgtat ggatgattag cgaaaatgct tatccacatg
    23701 gttcaccttg gacgaaaatg cagtttttgg cagacatcca acagcctcac tctgattatc
    23761 tgcttttagc tgaagataac cagatctgtg gctttattgg atactcgaaa gtaatagatg
    23821 aagtcgagat cacaaatatt gctgttgcag tttcagaaca aggaaaaggg catgcacggc
    23881 atttgttaca attactgatt acggaacaaa taaaagattc gctgcatgtt tttttagaag
    23941 tacgtctatc taatgaagca gcaagaaaac tttatgaatc agaaaagttt cgtatattag
    24001 gtaaacggaa atcatattat cgcaatccga cggaagatgc gatcattatg agcagaaagt
    24061 taaaaacgga gacgatgaaa tgaatgaaga attgattctg gcgattgaat caagttgtga
    24121 tgaaacgagt gtagcggtcg ttagaaatgg aacagagatc ttatctaaca tcgttgcttc
    24181 tcaaataaac agtcataaac gttttggtgg cgtagtacct gaagtagcta gccgccatca
    24241 cgtagaacaa atcaccttat gcttggaaga tgcactggta gaagctggtg tgtcggcaga
    24301 agatttatcg gcagtcgctg tcacttatgg accaggactt gtcggctcgt tgctgattgg
    24361 tatttctgca gctaaggctt ttgcttgggc acaccaattg ccgcttattc ctgtaaacca
    24421 tatggcaggg catatctatg cagcccgtct ggttaaacca ttccagtttc cattgatggc
    24481 acttcttgtt agcggaggac atacagaatt agtctatatg caagaagatg gatcatatga
    24541 aatcattggg gagacaagag atgacgcagc aggagaagct tatgacaaag ttggaagagt
    24601 attaggtctt tcttacccaa gcggaaaaga aatcgatcag ctagctcatc agggaaaaga
    24661 taattaccat tttccgcgag cgatgatcca tgaagataat tatgatttca gttttagcgg
    24721 attaaaaagt gcatttatca atttggtgca taatgcacaa cagcgtggag aagatctaga
    24781 caaaaatgat ttagcagcta gctttcaagc aagtgtgatc gatgtattga ttactaaaac
    24841 acttcgagct tgtcaaaatt atccagtcaa gcaactgatt atagctggag gtgtagcggc
    24901 caatcaaggg ttgcgtgaag gattgcagga tgctttggca actaaactcc ctgaggtaga
    24961 actagtgatt ccccctcttc gactttgtgg agataatgct gcaatgattg gagcagcagc
    25021 acatgtggaa atgcaaaaaa gaaattttgc tagctatcag ttgaatgctg atccaagctt
    25081 ggttttatct gaataaagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
    25141 ccaggaaaaa cggcttctca tgtttttgac ctggctgttc ttattaccgc gtatgcaagc
    25201 ttttgaacgc cgtttaatca agagttcgta agcctgtcgt ttaactctga ttcacaagga
    25261 acaatttcaa aaaacagctc ctcatgttta tgaacattat gacttgtgac cgaagagttg
    25321 acaggtacac accgtttaat cgaaataggg ctgtgacacg tttttgtcaa agccctattt
    25381 tttacatact tttaatcatt attgttattt tgctattttt tacagcttat ttatcattat
    25441 ccgttcctct gattgccctc actgagtaat cgtttattgt cgaggaatat cgaataatct
    25501 agatcggatg aggacaagac gcttcatcta gttaatctaa cagtattttt tgcaaacagt
    25561 gaatattaca taactgaaac acacatagaa aatctttgag tcttgtccag tgatctaaaa
    25621 ctgccggatc tttcagaagt tttagggagg aaaataagca gtgaccctga t
//
LOCUS       DACECY010000039        24269 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.040, whole genome shotgun sequence.
ACCESSION   DACECY010000039 DACECY010000000
VERSION     DACECY010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.040"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..570
                     /locus_tag="IWO38_001728"
     CDS             <1..570
                     /locus_tag="IWO38_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0313816.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            574..1215
                     /locus_tag="IWO38_001729"
     CDS             574..1215
                     /locus_tag="IWO38_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0313817.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1212..2129
                     /locus_tag="IWO38_001730"
     CDS             1212..2129
                     /locus_tag="IWO38_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0313818.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2126..2797
                     /locus_tag="IWO38_001731"
     CDS             2126..2797
                     /locus_tag="IWO38_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0313819.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3338..3826)
                     /locus_tag="IWO38_001732"
     CDS             complement(3338..3826)
                     /locus_tag="IWO38_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAQ0313820.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4027..4293)
                     /locus_tag="IWO38_001733"
     CDS             complement(4027..4293)
                     /locus_tag="IWO38_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0313821.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4293..4556)
                     /locus_tag="IWO38_001734"
     CDS             complement(4293..4556)
                     /locus_tag="IWO38_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0313822.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(4549..4821)
                     /locus_tag="IWO38_001735"
     CDS             complement(4549..4821)
                     /locus_tag="IWO38_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313823.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            5032..5217
                     /locus_tag="IWO38_001736"
     CDS             5032..5217
                     /locus_tag="IWO38_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0313824.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(5359..7182)
                     /locus_tag="IWO38_001737"
     CDS             complement(5359..7182)
                     /locus_tag="IWO38_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ0313825.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7780..10485)
                     /locus_tag="IWO38_001738"
     CDS             complement(7780..10485)
                     /locus_tag="IWO38_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ0313826.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10791..11738)
                     /locus_tag="IWO38_001739"
     CDS             complement(10791..11738)
                     /locus_tag="IWO38_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ0313827.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11928..12197
                     /gene="rpsN"
                     /locus_tag="IWO38_001740"
     CDS             11928..12197
                     /gene="rpsN"
                     /locus_tag="IWO38_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ0313828.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12373..12735)
                     /locus_tag="IWO38_001741"
     CDS             complement(12373..12735)
                     /locus_tag="IWO38_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0313829.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12746..13867)
                     /locus_tag="IWO38_001742"
     CDS             complement(12746..13867)
                     /locus_tag="IWO38_001742"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ0313830.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13883..14233)
                     /locus_tag="IWO38_001743"
     CDS             complement(13883..14233)
                     /locus_tag="IWO38_001743"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ0313831.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14363..15874)
                     /locus_tag="IWO38_001744"
     CDS             complement(14363..15874)
                     /locus_tag="IWO38_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0313832.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16196..17548)
                     /locus_tag="IWO38_001745"
     CDS             complement(16196..17548)
                     /locus_tag="IWO38_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ0313833.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17701..18777)
                     /locus_tag="IWO38_001746"
     CDS             complement(17701..18777)
                     /locus_tag="IWO38_001746"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ0313834.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18973..20718
                     /locus_tag="IWO38_001747"
     CDS             18973..20718
                     /locus_tag="IWO38_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ0313835.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(20998..21435)
                     /locus_tag="IWO38_001748"
     CDS             complement(20998..21435)
                     /locus_tag="IWO38_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ0313836.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21419..22792)
                     /locus_tag="IWO38_001749"
     CDS             complement(21419..22792)
                     /locus_tag="IWO38_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313837.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23114..23602)
                     /locus_tag="IWO38_001750"
     CDS             complement(23114..23602)
                     /locus_tag="IWO38_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0313838.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23624..24226)
                     /locus_tag="IWO38_001751"
     CDS             complement(23624..24226)
                     /locus_tag="IWO38_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0313839.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6958 a         5163 c         4488 g         7660 t
ORIGIN      
        1 gcaagccgta tcgccatcat gagtgaaggc cgtgtgatcc aatttgatac accagaaaat
       61 attttacgtc atcctgcaaa cgatttcgta gaagagttga ttggagaaga tcgcctacta
      121 caagccaagc caaatgcgac aagagtcggc gaagttatgc taaatacagc aatcacgatt
      181 acaccggaaa aatcattaca agaagcaatc aaactgatga gggaaaaacg tgtagatacg
      241 ttgttagtta ccgataattc gaatgtatta aaaggattta tcgatgtaga aacgttgaat
      301 aaaaaacgag gaaaagtatc cagtgtcggc gacattttga ataaacatgt tttttacgta
      361 aaagaatccg cctatcttcg tgataccttg caacgcattt taaaacgcgg gctgaaatac
      421 gtgccggtcg ttgatgaaca gaacaaagtc gtaggtatcc tgactcgtgc atcattagtg
      481 gatatcgtct acgatgtgat ttggggagaa gatcccgcaa ctactgatgt tgcagagtca
      541 gctactcctg aagaatctaa ggaggtctga cctatgcagc agttttttca tcagcacggt
      601 tcagaaattc tctcaaaagg ttttgaacat ctttatattt caggacttgc tttgatcatt
      661 ggtatcatca ttgctgtacc gcttggtgtc ttactgacac gttttccaaa agctgccacg
      721 attgtgattg gcttaaccag tgccttgcag acaatccctt ctcttgcttt attggcattg
      781 atgatcccgc tatttggcgt aggtaaatta cctgcgatta tcgcactctt tatttattca
      841 ttattgccga ttttacgtaa tacgtacatt gggatgaaaa atgtcgattg gaattatcgg
      901 gatgtggcaa aaggcatggg gatgactgag ttccaatcca ttttttccgt tgaattgccc
      961 attgctatgc caacgatcat ggctggtatt cgtttagctg ccgtttacgt gattgcatgg
     1021 gctacacttg cctcttatat cggcgctggt ggtttaggcg atttgatttt tagtggactg
     1081 aataattacc aaccagatct cattttggca ggtacgatcc ctgtaacaat tttagcacta
     1141 ttggctgatt ggcttctagg tctcttggaa caccggctga caccgatcgc acttagagag
     1201 gagaatgaag aatgaaacgt atactaaaaa tgaccatggt tcttttctct cttcttatac
     1261 ttgcgagttg ttccttccct ggtcttgcca gcaatacaga tgaagacacc atttcgatca
     1321 caggtggaat cacttcagaa gcacagatct tagcaagtct agttgccgga atggttgaac
     1381 attatacaga taaaaatacc gcaatcatca acaatctggc tactacaact atcaaccatc
     1441 aagcgatgat gaacggagat gcttcaattt cagcggctcg ctacacagga acagatttaa
     1501 cgactacatt gaaccttcct cctgaaaaag atccgaaaaa agcgtttgct accgtgaaag
     1561 atgaatttga gaaaagatac ggtcaaacgt ggttcccgtc ctatggtttt gaaaatacgt
     1621 atgtctttct tgttcgaaaa gacaccgcac aaaaatacca tctatctaaa gtcagcgatc
     1681 tgaaaaatgt agcggatgaa ttagtagctg gtgtagatac ttcttggatc aatcgtaaag
     1741 gagacggtta tgacggcttc caagaaactt atggcttctc cttcaattcg atattaccta
     1801 tgcagattgg acttgtttat gacgcagtcg aagcaggaaa aatggatatt gttcttggct
     1861 actctacgga tggacggatc gccagttatg atctagtcat gttggaagat gatcgtcaat
     1921 tcttcccacc atatgatgct gctccagttg tagacaacaa gatattagct gatacgcctc
     1981 atttaaaaga agccttaaat aagttaggtc ataccatcag tacggaaaaa atgcaggaat
     2041 tgaactacga agcagataat aatctagtgg aaccatcagt agtagcacaa cgtttcttac
     2101 aagaaaacca ctattttgaa gggaagtgaa gcaattggat aaaatgaatg tctttcaaca
     2161 atttttctat tatttccagg aaaatggctc ctatatcttt gcacaattta tccgtcactt
     2221 cctgatttct atctatggtg tcctctttgc agcagttgtc gggattccgg ttgggatcat
     2281 gatttctagg agacgtaaat tagcaaactg ggtgattcgt ttggcgaata tcatccagac
     2341 gatcccttct ctagccatga tctccatttt aatcattgga ttaggcctgg gagtcaatgt
     2401 cgtgattgtt actgtatttt tgtattccct cttaccgatc atcaagaata cgtataccgg
     2461 gatgatccaa gtagacaaaa acatccttga tgtcggcaaa ggcatgggga tgactgctag
     2521 acaacgacta ttcatggtag agttaccact ctctgtttct gttatcatgg caggcataag
     2581 aaacgcgtta gttgttgcca tcgggataac cgcgatcggt gcattcgtcg gtgctggtgg
     2641 tcttggagat atcattatcc gaggaaccaa tgcaacagag ggcacatcga ttattttagc
     2701 aggtgcattg ccaactgcat taatggcgat catcacggac tggcttttag gtattttgga
     2761 aagacggcta gatccagcca gccgtacatc acgctgatgt tattgtaaaa aacaaaagct
     2821 tctgcgacca tttgttggtc gcaaaggctt ttttagttct gtcattttta aggcaataat
     2881 ccactctgat tcttccctcc ttatgggcaa agagttgctt ttcttagcta tttatccatt
     2941 ccgtatactc caacttcata ctgccaacgg gttcatatct gcatccaccg actaagtttt
     3001 tttcttatcc gctaaggcat tttgccaatc cattttccca tcactcgtca tggaattcat
     3061 accacatgat ctctaaatac tcgtctgctt gttctttgaa ttgggcatca attcttacat
     3121 cctttggcgt ggctttcatt taagcctttt ccatcagtcc tcgattgttc gatgccttca
     3181 gctgcatcat caaaactggt gactgccgta tcggtaactt cgatcatctt ttatacaatc
     3241 gggaacttgc catcctcatc cttggctaaa aacatatcta gccattccag cttctcgcca
     3301 tgggaggttt gcggttccta aaaccggtac acgattctta tggactgcat ccgtcgcaac
     3361 tagacttggc gatacgatga tccttcacca gaagagcacc cccgtaaacc aattgatcga
     3421 tatattgcca ataagaaaag acattggcgt aaaagatatc gatccctcgt gatgcattgc
     3481 cgttgatact gctattcatg gccgaaagcg ccacgacttt aacttcttcc gtttgagtga
     3541 cattcatgtt cggcaatgtc gctttggata cccgctctgc taaaggaacg acactggtat
     3601 tgtatttcgt atctgtatct tttccaaaag accatgccaa cgaatcctct gggaaccaat
     3661 aagacgactc cggttgattg tccattgctt ttttcaacat acttttggct gtttctcgat
     3721 aaacgacatt tccaattgtc ttcgcctcac tttggcgata atcgatggtc gttgccactg
     3781 ccaacactaa caacgatgct ccaattttac tgattttcct tgtcacactt cttcctcctt
     3841 gttttacata aacccgtcaa cattccattc ctaaactcaa acaatgaccc ttcaaaggtg
     3901 gagatatagg aacaaattaa agaaatcatg caataaatcc aagaaggttg gttttagaac
     3961 aaaaaaagag tctccttttt ggggagactc tttgttaact atctaaaaag gttggctctt
     4021 ctcacttcac tcataatgtg tccaggcaga ataaatttct acaactttat tttcctcatc
     4081 aactttgtaa accactcgat gctgtctatt cagcctgcgt gagtatctgc cttcatgtgt
     4141 aggtctcagc ttttcaaaag actgcgtggg catatatgga tcttccttca atgtctgaat
     4201 cacttcttca aattgtttct ttaaattcgt ttgcttgatt tttcttaaat ccactttcgc
     4261 agagtttttg attgcgactg tatagttact catcaaagag cttcccaatc aatatcatcg
     4321 atattcgtaa agctactgtc gtctttttcg cggtctcgta ctttatctaa agtacctgtt
     4381 tgctcaagaa aaagggtttc ttgaatcgct ctccagtctt ccagcccaat caatacagca
     4441 ttgtttccac tacgatcact aataatctcg atttcagtat gattttcatt tacctctttc
     4501 aataactgat aaaaattttt acgagcagtg ctaggattca tcgttgtatt agccattgtc
     4561 tctcctcctt tttctgatat aattatagcg tacttgatta agtacgtcaa aaaaatacaa
     4621 taaagcactt aaattcagca aacttttcct cttttttaca tagagaaaaa gacccctttt
     4681 tagggaggat ctttgtccac gctcacttct tactccttta cgactagcgg tagccaaacg
     4741 taaagtaatt ggtaacaacg tatcatcttt agcaaaggtc gaatccaaag cctgtgagtc
     4801 tatgcaacaa caccaataca cgaaggttcc tcgttgcagt gtgacttgat tgatatcttg
     4861 aataacctga ggatacgcac gaaagatttg taacggttat tcaaaactga ttttggccgt
     4921 caagtcttct actgcctcaa aaatgattgc ctctttcgtc tcctgtcatt catgttcact
     4981 atcgatttga tacttgctta gaccgttggg tttgtgaatc accaattaaa aatggcactg
     5041 agttatggta acaaaaaatg gtcacttgac gaggctgaga ttcctactta tgaagacaca
     5101 tggcacttaa gagtcgaggt aaaggaagaa gctgaaaata taggaggctg tacattattt
     5161 ggcaaaggat tcggaaatta tgatcaagac atcaaaatca aaatgtttta cagctaaata
     5221 ggaagaataa taacatttca tacgctgttc ccatctttaa tggcttatag agggcacctt
     5281 tagctgagaa taagacaaaa cagaccctct agcttcaatt aagagctaga gggtctgttt
     5341 ttgattttaa aacttggttt atttcttcaa atggtagcta taggtgctga caacgatatt
     5401 ttcacgccaa gacaatcgca atcttaaccg caaacttgtt tggttatgga gaatattctg
     5461 ccagcgtctg tttggtacaa attgaggaat caacacagta gttgtatagt tttgtttcgc
     5521 tgcattttta cttacgatat ctacataacg gataattgga ttttcaatcg agcgataaga
     5581 agagtggacg attgaaaaac ggacatctgg gaaatgcttt ttaaattctg cttcgatttc
     5641 tttttctttt tcgacatctt cgtctagcga aacatgcatc gcaacgacat aatcaccgat
     5701 cgaacgtgcg taattcaacg caccgatatt tacccgtgtc acattcccga ctaatacaag
     5761 aacggtattc ccgtcataat catgtagttt tgcatcattt tctaaacgaa gctgttctgc
     5821 tactttttta taatgatcat ggatcttata aaaaatgaac ataacgattg gcataatgat
     5881 gaagaaaggc caaatgtccc ctaaccgata gacaaacaaa atcgcaatga ttgcatatga
     5941 gatgaacgca cccgtaatat tggcaatcga tttgcttaac catctttttc cttctttttt
     6001 ccatttgacg accatccctg tttgagataa agcaaatgga atgaacacgc cgatagagta
     6061 taacggaatc aagcgttctg ttgagccatg gaaaataaac agcaatacga tcgaacctag
     6121 cgctagtgtg ataatcccat tggaatagcc tagtcgatcc ccacgatctt gatacatgtg
     6181 aggcatgaat ttatcttttg ccagattgta tgcaagaact ggaaaagcag aaaaaccggt
     6241 gttagcagca actgctaaaa tcagcgctgt agcaaattga aggacgtaat acaaaatacc
     6301 atgtccaaag acagctttac caatttgaga caaaacagtg acttcttttt caggaacgat
     6361 cccataccaa tagttgataa atgtgatccc cacgaagaag aaacctaaga tcaatgccat
     6421 catcgtcaat gtagcagcgg cattttttgc tcttggcttc ttaaagaatg gtacagcatt
     6481 actaatcgcc tccaccccag ttaaagagga agaaccagaa gaaaatgctc gtaaaatcaa
     6541 agcgatagaa acacccggca cagccgctcc tggaagtgca gtagcttgca atggcacagc
     6601 acctgtcacg atattatata aaccaacaac gatcagtaac gagatcacaa ggataaatgt
     6661 gtacaccgga aataatagga acgatgcaga ttccctcagc ccacgaagat tcagcatcat
     6721 cagtagcaaa acgattgtca cagaaatagc gacttgatga ccgtagagtg ctggcaccgc
     6781 tgatgtgatc gcttcagcac ctgctgagac agatactgct actgtcaaca tgtagtcgat
     6841 caataaagag ccaccggaaa tcaagccagc attctttcca agattctcac tgctgacgac
     6901 atatgctccc ccgccatgag gataagcatg gatgatttga cgatatgata gcgttaatga
     6961 aatcaataaa ataataacaa aagcggcaat gggtagcgaa taccagatgg ctgcagcaga
     7021 caaagcgact aaaacgacaa cgatttgttc cgtaccatat gcaatagatg ataacgcatc
     7081 agacgacaat aatgccaaag cagcaaatcg ggttaatttg tgctcatcat tttcagcaga
     7141 ttttaaaggc ttacctacta acaacctttt taagtaatcc aatttctaaa ctcctttaca
     7201 cacaatttta cttcaagttt tatagtcgaa aatttatagt aaacaagatg ttatagtacg
     7261 ctcactgacc ttaaaagtca ataaacttta agaaaataat tggaaaaaaa cagaaaaata
     7321 tcaagcgctt tttcaaacgc tttaatacag caaaaatcta acattttttt atgggctttt
     7381 tctttgaatt ctataataaa aggatagaac ttgacttaga aaatcaagtt ctatcctttt
     7441 tgatatctta acttttggaa gagagatgta aggctgcttt ctacccatca ttcttactac
     7501 tcgaagcata gcgcagttgt cacaacctct tgataaacgg tgtttaaaag cttgcatctg
     7561 cggtaataag ctcaaccaaa ttaaaaacat aagaagctgt ttttatttgg tcgttcttat
     7621 tacttgatgc agagcagctt ttttacaacc tcttggtaca cggtgttcaa tcagctgacc
     7681 ctcggtatta gctcaaaaaa acggcaaaat atgaggaact atttttccat ttttttctct
     7741 aatactcagg tcaaaacact gatttcacaa cctctttttt taatgaaaaa tatggctctc
     7801 atgcgccgct tctttgaatt tcgtcatttt ttcttcgatg aacggataga ttgccgttcc
     7861 tacgattcca aagacaacaa acaaaccgat catgatccag attgctttcg tagcatttgg
     7921 ccaataaatt cctcctgcag attcccgtaa caagtttacc gcatgagtaa atggcaagaa
     7981 cggattgatc atttggaaga atttccctga gacttggatc ggatagttcc cgcctccgcc
     8041 agagatcgac agtactaaga tgataatagc gatcccttta ccgacattat cgaataatgc
     8101 agccaagaca tagacgatca tcatgaacgc taaggcgatc aagacagcaa atgacacgct
     8161 gtagaccgga ttttggacat ctactcctag taagaaaata ttccctaatg taacgatcaa
     8221 gctttgcgca atcgccattg tcaaaaatgt cagcatacga gcaacgaatt gttctcgttt
     8281 cgtaaaattg tttcggtctt tctttcctaa atagtattct gtcgtagcaa cacttgaaag
     8341 aagaacagcg ccgacccaca gacatagtgc agtataaaac ggggtgctag ctgaaccgtt
     8401 atttgcgatt ggataaagcg cattggtttg tagctcaaca ggttgtgtaa agaagtcact
     8461 ttcttttgtt gcgtctagtt tcaataattt gatgacttcg cctagatcga cttcttgttc
     8521 gccttttcgg atcgcgtctg ctgctttttg gataccagct ttgatatttg gccagtcatt
     8581 gttgatcaag tccgcagcag cgttcacagc tgtttcgact tctggcattt tttcatttac
     8641 tgttgtcaat gtaccggtga tttcattttt tagtgctggc caatcatttt ggataaaatc
     8701 agcggccaat cctaattttt gctgaatgat cggtaattca ttgttgtata ggtctgcccc
     8761 ttcattgatg ccattgacga tggtatccat atttccattc agcattgtat tggcatcatg
     8821 gatttcttgt ccaattgcag gcatttcttt ttgatatttt tctaaaattg ccactgcatt
     8881 cgttacagtt gctttcgttg agctgagaag cgaatcaaag tcgatctggt ccgctttgct
     8941 cagtgcgcct tgtgcagtcc caatcgtatc gatgacttgg tctagtagag aagtgacagt
     9001 ggaactgatt tgacctgtat tgataccagc aatgatgcca ttgatattat taacaacggc
     9061 atcaatttga tctagcaatt catcaatagc cttcgtgtct tcattttgaa cagctgtgtt
     9121 caaagcagat aggcggtttt gcagattgct tagcaagggt tttaattgat ttagctgatc
     9181 aataattggc tgtaatttcg tatttccagc acttgtttgc aatttagtca ggaactcgac
     9241 cagtttatcg attgcatttt gttgttgaga tattccgtca ataaagccgt tcaatacatt
     9301 gctgatgttt ttcttttcat catcagtcag cttaccatct tcaatagctg ttcttacagt
     9361 atctattaac gaacctgtcg ttgttgccac ttgtccaatt gacttcaatg taacatctac
     9421 actgtttgtg atgcttggta tcgcatcttt cagctgagag gcacctgatt ttgtttggtt
     9481 tgctagatca cttgcctgat tttctaaatt tttcacatct ggcaagatat cttgtacttg
     9541 ttggatgatc gtcaagcctt gttttgcttc atcgattcct tggttcattg tctcttggat
     9601 cgaagcaaaa tcgccgtcga tctctgctag ttgcttaccg gcattttgga tctctgggat
     9661 tttttcttgc aaatcaagga tgatttttgc ttgatctttc aactcaggca ttttgtcatt
     9721 caatgcaacg actttttcac ccatctcatc tactttaggg atgtaatcca cgaattcatt
     9781 tgctttattc agtttttcct tgatttcagg tagttgactt tgtaattcga ggacttgttt
     9841 tgtgtagccg tcgatcgtgt ccaaattttc atctgttgat aaaatcatat ctttaacttt
     9901 attgatactt actaggttcg tatcgatgtc gtaacctatc tcattgaata ctttcaataa
     9961 agtcgaactg gctgttttga taaattcatt tgtgatttgc gattggatcg aacttgctcc
    10021 tttatcggtg attttcggtg caatcgcatt gattttttca tttaccgtat attcgatttt
    10081 tggtttcttg atatcaccac tagtaaaact cagcagatct tctgagaaat cctttggcaa
    10141 gtaaatacct gcgtaatact ttccggaacg gactccgtct tctaattgtt cttttgaatc
    10201 aacgaattgc catcctaatt gtttattttt atgaagcgat tcgatgactt gctccccaat
    10261 ggccacttct ttaccttgaa actctgctgg cttgtctgca ctgtaaacag caattggcag
    10321 ctctccagta tttccatatg gatcccataa tgctttgatg ttaaaccacg catatagcga
    10381 agggatgatc atgatcgcga tgatcagaag tgttgcaatc gggtttttga aaatccgttt
    10441 ccagtcaagc aaatataatc gaagcgtatt tttgattgac tgcatagttc tcccccttct
    10501 tcttttttca gctgaattac tttaccatgt atttcatact tttttcaatc ggcttttttc
    10561 aaaaaagtcg aatttttgga catatcacgg caattgtctc attgtaatct cttacatatt
    10621 gagaaataat taaggattac agcctttact ttatcatgaa ataaaaggga aagataattt
    10681 tatgccaaag tttttatcta ttatacgtat acgcaaaaaa gctaaaacat tttttgtctt
    10741 tgcccctaca aaaatgtttc agcttttttc gttttttatt cctgtatttc ttatttttcg
    10801 aatgacagga tgattttccc agcaggagga tgtccttcaa ctagctgatg tgcttggatg
    10861 acatgcttaa gtgaagcaga cagattcatt gaaataaaaa catgaaaggc tcctttttgg
    10921 taggctttga ttatgccagc aaaggcttct gcatctagat attcctttct tggaacaaac
    10981 gattcataaa agccttcctt tttttgccgc aaatctagat ctgggaggtc atttaatgca
    11041 acatacctgc cgcctggttt catgatctgg atgccggttt ctcctttgat acttcctttc
    11101 gtcgcatcga ttacaaggtc tgcctgatca gcaaattgca gacctggatt tgttttatca
    11161 taagcagcaa atgctgaagc tcctaatttt tttacctttt cctcattttt actggatgca
    11221 ctggttaaga ttcggattcc cttttcatgg agaagctgga tcagacttga cccgaccgca
    11281 cctgatgctc ctagtatcat tacagtatct gtcggctgga tttccgtcaa atggttgatt
    11341 agattataag ccgtaattcc tggcgtgacc attccggctg cttcttccca actcatttta
    11401 tctggaattt ttgctacttt tgctgatggc aacacgactt cttctccgta agtcccgccg
    11461 actgcatgga cagctactcg atcacctaca tgaacagttg tgacatctga accaatctct
    11521 gtcacaatac cagcgccatc attacctggt acataaggga atttcaatgt tcgtatctct
    11581 ttcatttctc ctaaacgcaa ggcaacatca taaggattca cactgaatgc ttttatttct
    11641 acacgaacat gtccagaacc gacttctctg ggatgagcat cgatttcttc aaaaacatct
    11701 tgtgcttctc cgtaaccgtt aattgcaaat cttcgcatgg tcaccatctc cctttttctt
    11761 taagtttacg tttatgtctt tattttgaca aaaaaaatgt cttttcgttt tggcgcatga
    11821 ttcaaaacaa aatagttgtc ttttttcgcg taacgtgtta aattatttat ctaaaagcgt
    11881 aaagattacg atttacgcta ataaaaaata attggaggaa tttgttgatg gctaaaaaat
    11941 caaagattgc caaagcaaaa aaacagcaag aaacgattga acgctatgct gctttacgcc
    12001 aggaattaaa agcgcaaaaa gattacgaag gcttgcgtaa acttccttta gatgctcgtc
    12061 cagaacgatt gaaaaatcgt gatttaatcg atggacgccc tcgcggatac atgcgtaaat
    12121 tcgggatgtc ccgtataaaa ttccgtgaat tagcacataa aggtttgatt ccaggagtaa
    12181 aaaaagccag ctggtaatcc aagtctggct gtaatgtaaa ggaggagggc gcatggacca
    12241 ctccttggat catcaaaaaa gatacgttcg cttcttctag cagaaatgct ggtactagaa
    12301 gcagacgtat ctttttttac gcacagctgg tcctttttta tatgtaaagg ctgcatggtt
    12361 acttcgctat atttatttag taacaggagt cagtgcttcg atacccacac taattttcaa
    12421 tgctcggtca acttctgcca taatgggtcg atccaaatgg cagacctttt ctttcaatct
    12481 tgatttatcg atcgttctga tctgctccaa caggataacg gagtctcttt ctattcctgt
    12541 tccagtagct ttgatcccaa tgtgagtcgg aagcttaggt ttagccattt ttgccgttat
    12601 ggccgcaaca atgatcgtcg ggctaaaatg gttacctaga ttattttgaa taaccaaaac
    12661 tgggcgtgtt ccgccttgct ccgatcccac gactggagaa agatcagcaa aataaatatc
    12721 tcctcttttt accataggat cctccttatt cttgatattc tcttggtatc cgctgtccta
    12781 atccgcaagc cacttcatag tggatcgtac ctaattgatc tgctatatcc tgcatagtga
    12841 tttctttgtc tgcatttttg ccgataagcg tcacttttgt tccgacagga aattcttgag
    12901 gcaatcggat catcagttga tccatacata cacgcccaat cgtttcacag tagttccctt
    12961 ctaccaatag agaaaaaccc tgcatctttc gtggccagcc atctgcataa ccgatcggta
    13021 tcgttccgat ccactctgct tcaggagtga tatatgtttc tccatagccg attccttcac
    13081 ctgctgggag ttttttgact tgaatcaatt cagaaaccag ctcaagtgct ggctgcaatg
    13141 gataaacttc cggtagtgcg tgcccagagg gattaagtcc gtacattgct actccataac
    13201 gaatcatatt gccgattgtt tcatcatgcc ataaagccgt tgcactattg ctgacatgta
    13261 catatcgagg aagttcggga agttttttca gcacttctat aaagcgttct ttttgcagat
    13321 tccaatatgt atcatctgcc tgatctgctg tagaaaaatg ggtaaataaa ccttcccatt
    13381 ctaatacacg actttctttg atgaatgcag ctgcctcttt tacttcttca ggtgtacaaa
    13441 atccgatacg acccatccca gtgtctgctt ttagatgaat cgaaagaggc gtctctagct
    13501 tacttttctc taaaacagca gctgctgctt gcaaccattc ccgtgtacct gcagttactg
    13561 ataaatcatg agcaacagct acagtcagtg cgtcgtaagg aaccatattc aagataagga
    13621 tgggctgagt aaatccagct tctctcaact cgactccttc atctaaattt gaaacacaaa
    13681 agcccgaagc tccacctgca actgctgcct cagctgtttc aatagctcca tgaccgtatc
    13741 cgttcgcttt tactactgca aacaattctt ttccttgcgg aagtcttgca acttcatttg
    13801 ctacattttc agttatagct tttttatgta tcactgcttt cgttgggcgg tgccaagcga
    13861 caaccatcgt ttttccttct ttctagtctt ccaaaataat ttgtgcaaaa gctgtttcat
    13921 ttgtatgagt aatactgacc catacattgc cttcatgagg tgaacgcgtc acaacaggct
    13981 gaccattttc attttttaaa atctcgacat cttgaaaggt aacttttccg atcccagttc
    14041 cccaagcttt agagaaagcc tctttgcaag cgtaacgccc gcctaaaaat tctacttggc
    14101 gatgaaaagg caaggactgg aatagtttca tttcatcaga ggtcaagatt cgtgcaatga
    14161 attttggttt ttcttcgatc agccgagtga tcctaggaag ttcgactgca tcgatcccaa
    14221 ttccttttat catatgcttt tctcttttca ataaatttag aacactctca ttctaccaaa
    14281 ttataaccat tttgccacaa ataagtccac aaataacaaa aatgaaagtt tctcttaaaa
    14341 aagagaaact ttcattttat gtttaatcgt tatttgaacg gattacaaat ccgcgttttt
    14401 tatcattgtg acgtttaccg ccatcttttt ggctgttatt atttttgcta taaccgcctt
    14461 ttgatttgtt tccacgataa ttaccgccgt cacgacgatt acggttgttt ccgccaccac
    14521 gtttgccatt tttattgtat ccttttttct gcattggcaa tgggcgttct ggtgtgatct
    14581 tcactggcac tgcatcagct ggatcttttg ctgtcgtttt tagcaaaagt gcaactaaat
    14641 cttgtgcaga atattcttct aataatttat ctgctgtttg caggtattta tctagaccat
    14701 tttctgcaag ttttgtttcg atttgttcaa ctgcagcacc taattgacct ttgaatgctt
    14761 ctttttcggt tggtggacgt aaagtcgtca tccgtttttt cgtcaagttt tcaatcacat
    14821 gcaagtagtc catttcattt ggtgtaacga aagtcacaga cataccgcct tttccggcac
    14881 ggcctgtacg tccgatacgg tgaacgtagc tttctggatc ttgagggata tcatagttgt
    14941 acacatgagt gacacctgaa atatctaatc cacgtgcagc aacgtctgtc gctacaagga
    15001 tatctaaatg accgctctta aatgaacgca atacgctcat tcgtttttgt tgagacaaat
    15061 cgccatggat tccttctgca cggtaaccgc gtgcttctaa tccgcgtgcc aattcgtcta
    15121 cacggcgttt tgtacgtccg aagacgattg ttaattcagg tgtttgaaca tcaaacaagc
    15181 gagtcatgat atcgaatttt tcgtattctt ttgcgcggac atagtattga tcgatcaagt
    15241 cagctgtcat ttctttcgct ttgatcttca catgatgtgg atttttcatg aatttaacgc
    15301 caatattctt gatagctggc ggcatcgttg ctgagaaaag taatgtttgt ctttgatcag
    15361 gaacttgtga aatgattttt tcgatatctt ccaagaagcc catattcaac atttcatctg
    15421 cttcatccaa aactaatgtt tgaacggttc caagtttcaa tgtgtggcgg ttaatgtggt
    15481 caagcatacg tcctggtgta cccacaacga tatgtggacg atctttcaag ccacggatct
    15541 gacggccaat atctgctccg ccgtaaacgg cttgtacacg tacttttttg tcgcgcccta
    15601 aacgatataa ttcttcttgt gtttggatag ctaattcacg tgttggtgcg ataactaaac
    15661 cttgtaattc gtgacgatct ggatcgattt tttctaacat tggtaatcca aatgcagctg
    15721 ttttccctgt acctgtctgt gcttgtccaa taacgtcttt tccagctaat gctagtggaa
    15781 tcgtttctgc ttggataggc gttgcttctt caaaaccagc gcgttcgact gatttcaaca
    15841 attctggtga taattctagt tctttaaatt tcaaatgaat cctcctatat ggttgttaac
    15901 tgaggatgta gcttttttgc taccccgtcg atttgttcgg tcaaagtgta ttgtttcttt
    15961 ttgacagtgg ccacgcacgt ttttatccat tcttagcccc tgtggcacca ccgaaaaatc
    16021 actttgcgaa gtttaggcat tagaaaagcc aaacgggtcg cgttcagctt tatcattatt
    16081 atgacatctg gcttcaacaa gttgtttcta tggcaaaacc agtccaattt ctcttcatct
    16141 ttcggcaata ggttattcta acatagtttt tcgttttctg caagtttaca gatttttatt
    16201 tcatttgctg aagttgttct accacttcaa ataagcccat gctgttgctg ccttttagca
    16261 taatactatc tgttgctgca agattttgtt tgatagcatc aatcaattta ttcttttctt
    16321 tttcaaaata gaacacaggt agttgggggt atttttggtt caattcttct tttagtgcat
    16381 gcatttgttc cccataaagg aaaatcatgg cgaatttctc aggatcgata tgttcactca
    16441 tttgcgcatg catatttaaa gactctttcc ctaattccag catatctgct aacacagcga
    16501 ttctgcgtcc ttttgttggc agatttttaa aagtatctaa taccagtccc atggctgttg
    16561 gatttgcatt gtagacatcg ctcaaaatag ctgcaccatt tcctgccgtt agccattcgg
    16621 ttctgttttg agtgatagaa acatgggcga gcccttggcg tatctcaggt atggacagtc
    16681 caaagaagcg cccgatcgta tacgcaatca aagcattcgt tacattataa cttccaggca
    16741 gcgggatcgt aaatgtttct ccttctacta caaaatcggt ttgtgctttt ccttttgctg
    16801 tcacttccgc atgaacatcg ccatttccaa taccgaatgt tgtgactgtt tgtactaatg
    16861 ttgccgttaa aggtgtcagc aacggttcat ctgcgggcac aacgagtaat ccgtctgata
    16921 caagtccgtc agtgatttcc attttggctt tagcgattcc ttctcgcgac ccaagatttt
    16981 ctatgtgtgc ttcaccgatc attgtgattg ctgcagcttt tggttgagcc ataagtgaaa
    17041 gttccgtgat ttcattggcg tgatccatgc ccatttccaa aacgatcttt tctgtatctt
    17101 ggggcatgtg caaaatcgtg taaggaagac caatattatt gttgtaattt ccttgcgtct
    17161 tatatgtttt gtatttttca gacaagacag acgcggttag atctttcgtg gtggttttac
    17221 cattgcttcc tgtaattgca acgacttctg cgcctacttt ttccaagtaa taggttgcca
    17281 attgctgcat ggctttcaaa ggatctgcta caggaataac tggaaaattt tttggagctt
    17341 gttcaacagg caagctccaa aatgaagcag atgctccgtt tgtgatcgct tgctggataa
    17401 attcatgtcc atctcgcgta ccagcaagag gaacgaataa acttccttcg gttatcttcc
    17461 ggctgtcaaa ttcgacattg gtaatctttg tcagtgcaga gcattcgctg cccacggctt
    17521 gtgcaatttc ttgtattgtt agattcattg agatctctcc taaaaaaggt tgtgaaaaaa
    17581 gcgtttggcc taaacggcgg gttcgcatga ctctcacttc caagtaaatt tctctattcg
    17641 tatttagaag ctagaatgaa cgcggttacg caatcactcc agcctcttct ttatttctct
    17701 ttattcattt ttttcaaaaa aagattgacg ctgatggtat cgattcattc ctaactggat
    17761 cagttcttca atcaaatcac cgtatttcaa gcccatattt tcccataaga gtgggtacat
    17821 actgaactcc gtaaatcctg gcatagagtt taattcattc aggaataatt catttttatt
    17881 tgtcaaaaag aaatcgcacc ggctcaatcc gcttccacct aacatcgtgt aagctaactt
    17941 cgcgtactct tgcgcttttt gataaacttc ttctggcact tcggctggaa tctgcatttc
    18001 gattttatta ttgatatatt ttgcttcata atcatagaat gctacgtctt ttacgacttc
    18061 accaggcaaa gtcgtccgaa cgtcttcatt tcctaataca gcaacttcga tttcgcgcgc
    18121 ttcaattcct tgttcaacga ttgctcgaga atcatactga taggctgttg ctaaagcatt
    18181 ttgcagctct tctcggtttt ctgcttttgt aatgccgaca ctagaaccca tattcgccgg
    18241 tttgacaaac atcggataaa gcaaagaacc ttcacattga tcaaatactt ttttaggatt
    18301 ttctttccat tgattcttaa gtactggtac ataaggaact tgcggcacac cagcagcttg
    18361 taaaatatac ttggtcatga ttttatccat ggcacatgca ctggtcaata cgcctgcgcc
    18421 gacataaggc atattcaatg tctctaagaa gccttggatc gttccatctt ccccgtttgg
    18481 cccatgtaaa actggaaaaa cgatggctcc ttcttctttg atttcgcccg gattgatcac
    18541 ttttcctgta aagccttcct ctgtctgtcc acttgggtcc catgaaagat gcaagacatc
    18601 tttgctggca ggtttttctg ttagtaatgg acctttgacc cattgtcctt cttttgtaat
    18661 aaatacgagt tgaacttggt aataattata ataaatggca tttaaaactg aaaatgcgga
    18721 aagaatggac acttcatgct ctgcgctgcg tccgccatat agtaaagtaa tcttcaaaag
    18781 attgtcctcc tgtattctaa atcgttcagt gatttactct catattttag cacaaaacaa
    18841 aaataaacga ataacttatt tataaaaaaa cgagccagct tctaaaagaa cgagcgtcat
    18901 ttattttttg tcagctagat tatttgtccc gttttttata aaaagttatg ataaagaaaa
    18961 ggagggatga tgatggcgca aacaaatcca cgcagccgct tggcagaaat cattcgagtg
    19021 ttcatcaatt acaatgtagt gccaaacttt gtacagcaaa aaaatcccga acaggtaaaa
    19081 aaagcctttg aagaactagg ccccaccttc atcaagatag gacagatgct ttctgttcga
    19141 gaagatttgc tttcttctgc cttcactcaa acattcaaaa cattacaaga tagtgtacct
    19201 agcgacacat tttcaacggt caaaaagacg atcgaaacag aactttctct ttcattatca
    19261 gatatattcg atgatttttc aaaatcgcct tttgcctctg cttctatggg gcaggctcat
    19321 cgtgccaagc tgaagaacgg tgacgcggta gtcgttaaga tccaacaccc taacatcgcc
    19381 gaagagatcc gacttgatct tcaactattt gaacgagcga tccctttgat caaatacatt
    19441 cctgaaacta gcgtagttga tctaaaaggg gtactccaag aagtaaaacg ttccttgatc
    19501 aatgaaatgg acttcttaaa agaaagtcaa aatggcgaac aattctatca aaagaacaat
    19561 ggctggaaag agatccgcag ccccaagatt tatgacgctt tttgttcgaa aaaagtgatc
    19621 gtgatggaag aaatgtctgg taaaaacttg aatcatttaa tgaacgccga aaacaaaact
    19681 gaaactttta tcactggaat tcaaaacaag cagctgaaac aagaagttgc aaagctttta
    19741 gttgagaact ttatgaaaca ggtgtttgat gatggttttt tccatgctga tcctcatcca
    19801 gggaatctgc tattccatgt actaacaaaa gaagaacaga cacaagctag ccgaaaaact
    19861 gaaacagtcc atgaaaaaga gttcggttcc tttgcttttc gagcctccac ttctgcagaa
    19921 gaccctgttg cgccatatac catcaactac atcgattttg gtatgatggg gcatctttct
    19981 gcaggactta ggcaaaaact cacacaagcc gttttagcgc tgtatacgaa agatgcttat
    20041 cgaatcgaaa aagcggtcct tcgactttgt cagcaagaag gctcgtttga tgaaagtcgg
    20101 ttccaccaag aactgacaag ttttcttgaa cagtattatg attctcctat tgatgaaatc
    20161 aacttgcaag aagtattcac acatgtcgtg acgatttgtc atcaaaataa tctgcagttc
    20221 gatcgtgata tcacgctttt gctgaaagca tttgggacat tagaaggggt gatccgcgtc
    20281 ctcgatcctg aagtttccct catggaagtc gcttcgcctt ttgctcaaca ttattttctc
    20341 actcatctgg atgtagaaga tacattgaaa caaagcggat tagacttatt ggaagggatg
    20401 aaggctgcgc ctaagatccc tcaacagctc caccatctat tagaaatgtg gacttcggga
    20461 caaggaaaag tcaatttgga attgaaaaag caagacaaat tattgagcag gatcgaaagt
    20521 atgatcaatc ggcttgtttt cggtatgatt ttagctgcat tgatcgtagg gtcctccctg
    20581 cttgtccaag cagcacctgt agaaaacgca gaggtcgttt ccttattagg gatatttacg
    20641 tatgctattg ccgcattcgt cattattttt ttggcaatcg atgcattgat ccaaatgtat
    20701 aagaagagga aaaagtagaa aaaggttgtg acagaaagaa tcagcttcaa gaaataagaa
    20761 ggggttttac ataaattgtt tctcaaattt ttgtgaaaat tcggcttatt tttcgaagag
    20821 gctacttctg aaacaccgag aataaaaaat gtgaaaaagc tgttctgcac ccagtaataa
    20881 gaacaatcaa aaagggaaca tggaacacat ttatcctgtt tccctgttcc ctttttgatg
    20941 tattcgagca taaataaaat accttcgtta cctactatct tctgtacttg gtaaacctta
    21001 gtcaattgcc ttttttaacg cttgacgaaa agacatatga tctttctccg taaagggttt
    21061 tggacccttt gtccttatgc cagcttttct taactttcca ctatgataac gttgttctag
    21121 aagcgtgccg atattcgctt cgtcaaattc ttttccagag tgatggaaca ctcgcacacc
    21181 cttcggcaat acaagtgaag ctaaaccata atcttgcgtg atcaaaaagt cattcttctt
    21241 aaccataccg acgattttat aatccgcact atcagctccg cggtctacgt agacgatttg
    21301 gacatgatta gcgtattctt tggtcgtata atggtccaca ctggtcacga taaccacgtc
    21361 aagaccatag gattctccta attttatgac atcttctttt actggagaac catctccatc
    21421 aatgaatagc ctcattcttt ttcgccttct tccataaatg ataaacagtt actaatccgc
    21481 tcagtaaaag accaacattg atccattttg ccaagataag cagttgtaca ctttctatcg
    21541 gcactgattc atggttcagc gtgatcaata atagatggat tggctctccc atttcttttg
    21601 ctaaaacaaa caaaagcgtc atcaataaaa actggtggat cacactccct ttattttgag
    21661 tagtatactt gacaacgatc cggcgatcca ttggaatagc tgtatcagtt tgataatatt
    21721 ccttatttag ccagctgcct gttactacgt gccagaaact aagcagaaag acacctatcg
    21781 caaaaccatt tggaatcaag aggaccaata aagaagaact cagtccaagc aattgcatag
    21841 ccaatggaga ccatgttaaa cgtttcaaaa tccatggtcc gactattttt gctgcaatca
    21901 agcctgctgc ataaggcaaa tacataaaaa tcgaaagttt ttctaatccg tacagtatgc
    21961 caacataaaa actgctaaat aaaaaaagga aattccctag cattccattt agaaaagtaa
    22021 acagatttag ccagcttggc aacttccatt ccttcttctg gtgtcctaaa acaaacacga
    22081 acaagatgaa cagcagcaag aaaccataaa tagcaagatc aaaaagcctt tcatcgaata
    22141 acaatcgggc atttctcaaa agaaacaata agccaaagaa tatcaaaaat aaaacaagct
    22201 ctcgcgtagc taccagattt cttttgatgt ctttgaaatc cagttcgtaa cgcggataat
    22261 gactaaccgt atggtaggcc atgacgtaaa aaagtgtata aatagtaaag cttgctgcaa
    22321 tctgagtcgg tgcaggcaac tcaatcgctt gatacaggag aaatagccag atagctgcaa
    22381 ataagtactt tttcccatcc atattgataa aaccttgcgt cttttcgtga aaattgaccg
    22441 tggtattagc aggtacaagc catgctgcac tcagccctag caaaacactt gaaaaataaa
    22501 ataaaggaaa ataaaatacg cctaatattc caagcaatga accagcacca ccaatcagca
    22561 gagcgatcat cagcaacgtg taactatcta gaccaaatcg tagactgttg actaaaaata
    22621 gtccagtcat ccgaaaggca taaaacagcg taaatggcaa aatcgtcagc caattatgcg
    22681 agtctgttag tcccaaaaat aaaagataag gaataaaaat aaccgtgtta cttaagataa
    22741 agggcgttgt ttgtgtaaaa ttacgcccaa atcgtttgag tttttcgaac atgacaatgg
    22801 ctccttaaac agtcttataa ctaatctgct cttcgatttt agcattaaag gggaaataaa
    22861 ccaattttac agaatatatt cttgtaaaaa aaaatacaga aagaagcata agcttctttc
    22921 tgcattttta cggtacttga ttactcaagc agacaagccc ttctccacaa aagtcttgcc
    22981 cgacacccct gcatccggca ggtgataaag atagaaatca attatcctgt gcctccaaaa
    23041 tcaattagga gctctgtaaa ccagagtcct tctcgacccg tcgcataaat ttactttaac
    23101 acagttccta tgtttatgcg agagcttttt tcattttata atcgaccatt tgaaatcctt
    23161 gaccgatatc tgaaataagt tcttctgtaa tctcaaatcc ttcatgctta taaacctcga
    23221 ttgcttgatg attcccctga ttcactcgca aaaagatctc tctttccttt gtttgcgcag
    23281 caagttcttc ataccagcga aaaactgaag aagtcaatcc ttgaccacgt actttattta
    23341 gcaaatataa tttacttaaa taaatttttc catttatctc ttcataagca gtgtaaccga
    23401 cagtcttttc ttcgcttttt aataaaaagt aggacacacc ttctgtcagt tctttttgaa
    23461 tctgtttttt tgactgataa gttttcagca tgtaatccac ttgtttttga ccaatgatcg
    23521 gcacaaaagc ttccggccat acttcttgta tgactgccat cagctcatcc agttcatttt
    23581 ctgttacttt tattttttcc atacacccac gtcctttttt acattataaa tgaccttgcg
    23641 cttgatggaa taaaaagagc atgatcatgc tatacacacc caccgaccat ggtttcaaga
    23701 aaatcacaat ctgcataaat tccctaaaag ataatttcgt caaaccagct actagacaga
    23761 tcaagtcgtc tggcgcaaag ggaagcagca aggtaacaac tagtactttt ttaatatctt
    23821 tcgtttttct tgtcagcaat tcatcaaatt tctgatactt ttttggcgat aaaataagct
    23881 ggacaaaggc tacgccatag taacggacaa gcaaaaatcc gattgcttcc ccaatcacta
    23941 ggccaataca actatataaa agcccaaatc cattgccaaa catcagcatg cccgctactg
    24001 aggagatccc tcccggcaaa ataggaatga tcacttggat cacttgcaac agaacaaata
    24061 tcatcactgc atacttgcca aattgctgga taaacgcttg caaagacgtg ctcgatcgaa
    24121 aaatcccatg atgataagca taaagaatca acacaaagaa aaaaaacaga ccaataatgg
    24181 gcatgatctg aagacatttt tgtaaaactg atcctttttt agacatctat attccccaca
    24241 tcttttttca tttattaaaa ctgcctcct
//
LOCUS       DACECY010000040        23827 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.041, whole genome shotgun sequence.
ACCESSION   DACECY010000040 DACECY010000000
VERSION     DACECY010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.041"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(53..787)
                     /locus_tag="IWO38_001752"
     CDS             complement(53..787)
                     /locus_tag="IWO38_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ0313840.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="IWO38_001753"
     CDS             1114..1524
                     /locus_tag="IWO38_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ0313841.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="IWO38_001754"
     CDS             1517..2218
                     /locus_tag="IWO38_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ0313842.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="IWO38_001755"
     CDS             2218..3804
                     /locus_tag="IWO38_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0313843.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="IWO38_001756"
     CDS             3823..4044
                     /locus_tag="IWO38_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ0313844.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="IWO38_001757"
     CDS             4041..4802
                     /locus_tag="IWO38_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ0313845.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="IWO38_001758"
     CDS             5182..5691
                     /locus_tag="IWO38_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ0313846.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="IWO38_001759"
     CDS             complement(5766..6305)
                     /locus_tag="IWO38_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ0313847.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IWO38_001760"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IWO38_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ0313848.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7372..8169
                     /locus_tag="IWO38_001761"
     CDS             7372..8169
                     /locus_tag="IWO38_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ0313849.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8197..8832
                     /locus_tag="IWO38_001762"
     CDS             8197..8832
                     /locus_tag="IWO38_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ0313850.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="IWO38_001763"
     CDS             8852..9625
                     /locus_tag="IWO38_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0313851.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="IWO38_001764"
     CDS             10033..10830
                     /locus_tag="IWO38_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ0313852.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="IWO38_001765"
     CDS             10808..11221
                     /locus_tag="IWO38_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313853.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="IWO38_001766"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="IWO38_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ0313854.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="IWO38_001767"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="IWO38_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ0313855.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="IWO38_001768"
     CDS             15998..16558
                     /locus_tag="IWO38_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ0313856.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IWO38_001769"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IWO38_001769"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ0313857.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="IWO38_001770"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="IWO38_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ0313858.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="IWO38_001771"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="IWO38_001771"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ0313859.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="IWO38_001772"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="IWO38_001772"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ0313860.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="IWO38_001773"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="IWO38_001773"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ0313861.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="IWO38_001774"
     CDS             21905..22483
                     /locus_tag="IWO38_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ0313862.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="IWO38_001775"
     CDS             22535..23017
                     /locus_tag="IWO38_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ0313863.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="IWO38_001776"
     CDS             23134..23508
                     /locus_tag="IWO38_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313864.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7781 a         4285 c         5115 g         6646 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcacccggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaataa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagtt
//
LOCUS       DACECY010000041        23571 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.042, whole genome shotgun sequence.
ACCESSION   DACECY010000041 DACECY010000000
VERSION     DACECY010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.042"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWO38_001777"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWO38_001777"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ0313865.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IWO38_001778"
     CDS             complement(1172..1867)
                     /locus_tag="IWO38_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ0313866.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IWO38_001779"
     CDS             complement(1880..2596)
                     /locus_tag="IWO38_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ0313867.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3531
                     /locus_tag="IWO38_001780"
     CDS             2926..3531
                     /locus_tag="IWO38_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ0313868.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3856..4179
                     /locus_tag="IWO38_001781"
     CDS             3856..4179
                     /locus_tag="IWO38_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ0313869.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4361..5731
                     /locus_tag="IWO38_001782"
     CDS             4361..5731
                     /locus_tag="IWO38_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ0313870.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5861..7162)
                     /locus_tag="IWO38_001783"
     CDS             complement(5861..7162)
                     /locus_tag="IWO38_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ0313871.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7392..9383)
                     /locus_tag="IWO38_001784"
     CDS             complement(7392..9383)
                     /locus_tag="IWO38_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ0313872.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9527..9793)
                     /locus_tag="IWO38_001785"
     CDS             complement(9527..9793)
                     /locus_tag="IWO38_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0313873.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="IWO38_001786"
     CDS             complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="IWO38_001786"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ0313874.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="IWO38_001787"
     CDS             complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="IWO38_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ0313875.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13137..13700
                     /locus_tag="IWO38_001788"
     CDS             13137..13700
                     /locus_tag="IWO38_001788"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ0313876.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13953..14474)
                     /locus_tag="IWO38_001789"
     CDS             complement(13953..14474)
                     /locus_tag="IWO38_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ0313877.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14477..15319)
                     /locus_tag="IWO38_001790"
     CDS             complement(14477..15319)
                     /locus_tag="IWO38_001790"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ0313878.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15312..16037)
                     /locus_tag="IWO38_001791"
     CDS             complement(15312..16037)
                     /locus_tag="IWO38_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ0313879.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16213..16509
                     /locus_tag="IWO38_001792"
     CDS             16213..16509
                     /locus_tag="IWO38_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313880.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17077..18669
                     /locus_tag="IWO38_001793"
     CDS             17077..18669
                     /locus_tag="IWO38_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0313881.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18682..19422
                     /locus_tag="IWO38_001794"
     CDS             18682..19422
                     /locus_tag="IWO38_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0313882.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19590..20939
                     /locus_tag="IWO38_001795"
     CDS             19590..20939
                     /locus_tag="IWO38_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0313883.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21067..21657)
                     /locus_tag="IWO38_001796"
     CDS             complement(21067..21657)
                     /locus_tag="IWO38_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ0313884.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21804..23156
                     /gene="brnQ"
                     /locus_tag="IWO38_001797"
     CDS             21804..23156
                     /gene="brnQ"
                     /locus_tag="IWO38_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ0313885.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6750 a         4746 c         4490 g         7585 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 agcacaggtg aaggtggcga accagaagtg atcggttcaa cagaagaata atactgaaac
     3541 aatcaggtgg tgttagaagc ggcgggtcag cagcttttaa cacgcctttt ttatttctaa
     3601 tcgtttattt tcatatgggg cttccacttt tgtaaatggc tttacaaacg aaaaatccta
     3661 atttaccaag cttttcctcg catactttta tcgtaccttt ataaaaattt atactttgaa
     3721 aaaaagtatt caattattgt tgcatttttc ctgattcttt attagaatga accctcgttg
     3781 aagtaagtat ttctagttta tcctaggatt aattataaaa ataaaacatt cgttgattcg
     3841 aaggaggtta aacagatgtt tgaaagtttt gatagcagcc gcaaccgtta cgcttcagtt
     3901 ggtgtcgtat caagtttgcc agatgaattg attgacagta tatggtttat tattgaccta
     3961 gatcttaagg gagtcattcc attagaaaat atattaacct ttgatttgat cgaaaatcgt
     4021 ggaaaagtga cgatgcattt ttctcaagca ggaagcaatg tggaaatgga tattgatttg
     4081 ccgtttgcct attcgaacca atttcctgct caagtatacg cctatgatga cggtacgaga
     4141 gaaacgatcc ttttaccaag tgagattaga caatattaag gtgatagata aacgtggatg
     4201 tcccttattt tacagaccag gccaagttgt tgattctttg actcaattct tggcttcttt
     4261 ttatggagtt tcataaatta tcattgattt ctaattggta ttctcaggat aaacgctata
     4321 tactactaac taaattttaa agatataagg agtttaaaag atgcgcgatt taacaaaagg
     4381 aagccccgct aaactgattt tgatgttcac tgttccttta ctgatcggaa atgtttttca
     4441 gcagttctac aatatgatcg atatgatcat cgtgggtcag acgctaggta aaaatgcgct
     4501 ggcagcggtt ggtgcaactg gaagcctgac atttttgatc attggctttg cccaaggttt
     4561 aacagctggt ttagccatta ttaccgcaca acgttacggt gcaaaagatt atcggggtct
     4621 gaagaaaagt tttgctgcta gtgttgtcat tagtcttgtc gtcactgtga ttttaactgt
     4681 cctaagtctc ctgtttattc gtccgatgct tcaattgatg caaactcctc ctgaaatact
     4741 tgatcaagca caaacattta tttcaattat tttattaggg atctttgcat cagtcagttt
     4801 caatttgctt tctaatgtca ttcgcgcact tggtgacagc cgcacaccac tattctttct
     4861 gatcattgcg gtcatcatca acgtcgtact tgatttgatc tttattatct attttggcat
     4921 gggtgtggaa ggtgcagcta ttgctacagt aatcgcacaa gtttcttcca gcgtactgtg
     4981 tctagtgtat atcaagaaaa aaatgccttt gttacagtta cgcaaaaaag atttcaaatt
     5041 tgaaaaagaa gagtttgctg tacacctaaa tgctgctttg ccaatggcgt ttcaatcttc
     5101 gatcatcgct gttggtgcta tcgtgttaca agcagcacta aatagtctag ggacagatgt
     5161 cgtagctgct caagccgctg ctggtcgtat cgaccaattt gcaaatcagc cgatgatgtc
     5221 atttggtatt gcaatggcaa ctttcactgc acaaaactat ggtgcgaaag aatatggccg
     5281 aatcttgcaa ggggtacgtc aaactttact gatgagtgtc ggatttagtc tcttagcagg
     5341 tgctatcgtc attttcttcg gtcatttctt tgttcaattg tttgttagcc catcagaaac
     5401 acgtgtattt gaattagcac aaacctactt caatatcaat gggtctcttt actggatctt
     5461 agcagtcctg ttcattttac gctacacact tcaagggctt ggacaaagca agatccctac
     5521 gatcgccggt atgatggaat tgcttatgcg ttcttttgca gctatcgttc ttacaggaat
     5581 gtggggatac ccaggagcag cagcagcttc tcctttagct tggatcggtt ctgttgccgt
     5641 gttgctttac tcttatatgc gttcgatgaa acaattgaaa cacatgggtg aagaggaaca
     5701 tttcacatta gaacatgcag aagcaagata gccttgatta aaagaaatcg ttgcggctaa
     5761 aaatgaaaac ttttattgaa aatatcttct tcgtttaccg aaaacaggtt ttttagataa
     5821 caaaaaggct gtgacaaaaa atgtcacagc cttttttctt ttattctatt tttctttccg
     5881 tggactctac tttgaaataa tcgatatgtg catgctggta ttctaatacc gtgaaacgga
     5941 atgatgcaat ctcaatgatt gttcctgctt ttatttcttt atatcggcta gaagcaaagc
     6001 ccgcaagtgt agtaaaattc gtttcttcaa actctggaat ctctacatga aaataacgct
     6061 ggaaatcatc taatgttagt ttgccggaga tgatataaga gccatctgac tgttccgcta
     6121 tcaatgcttc gtccaaatct gtctcatctc ggatttcccc gacgatttct tctaaaatat
     6181 cttcaatcgt cactaagccg cttgtccctc cgtattcatc ttttaagata gcaatatgct
     6241 ttcccttttt tcgcatgatc gtcaataatt tttttatagg gatcgtttca gttgttacga
     6301 tcggctcttc aatcaactgt tctactgtca gctggtcatc tttataagat tgcttgatca
     6361 aatctggcaa ggtgacataa cctaaaacat tgtcttttga ttctaagata accggatagc
     6421 gagtatgtcc ttgttcaatc gaagctttta tcgcttcttt gacggtttct gaagcttcga
     6481 atacttgcat ggacgtacgg tctacttgta tatcttttgc caaggtatca tcaaattcaa
     6541 acacgttttc caaataatga tattcttctt tggtcaactc tcctttttca taagaattca
     6601 atgcgactaa tgtcagctct tcttgagaca atgtctcttc tccttcagat acgagacgca
     6661 cacctaatag cttccctagc cctgcagctg aatgattcaa tacccaaata aaaggaaaca
     6721 ttactttata aaaataatga agtggtttaa ctacgaacaa gacgaccttc tccgtattga
     6781 cgatactata gctcttagga agcagttcgc caataaccac atcgatatac gtaactaaaa
     6841 taaatcccag cgccacagag attgtactgc taatagccga actaaaaggt agtttaccga
     6901 tcactggttc tagtaaagcc gcgacagatc cttcaccgac ccacccgatg atcaaaccgg
     6961 ttagcgtaat tcctaattga ctagctgata aatagttatc taaatggtgt gtaacatgaa
     7021 gagctagctt ggcgttcttt acattttcct tttctagttg ttccaatctt gatcgtctga
     7081 ttttcactaa agcaaattca cttgctacaa aaaatgaagt aattgcaatc atcaaaaata
     7141 aaataatgaa attgataacc atattctccc gcctttctct ttttctattg tacccttttt
     7201 ctacaaaaag attgaaaata atgcgtattc atatttagga ataaaaaagg agatgcggca
     7261 tttttcagcc acatctcctt atcacttcaa aatcattgcg ttacttgttg ctaaacagct
     7321 ttttcatgac ctctgataaa cggtgttcaa gtagctgaca ttcgacatta gttcaggtca
     7381 aagcgctact ttcacaaccg ctctttcaac catgttttcc gagtatttcc aatgataagc
     7441 ggaacagttt catgttttac ttcgctgtac tctagcccaa accatgtttc tggcgaggta
     7501 gcgatttttt tgattgccag ttttgcctgc atgatttgac gttgccattt tcctagagtt
     7561 gtcacattgg aaactacttc atcaatcagg acaaattgga aatcgccaac ttctcgccct
     7621 ggtgtcagag agtatctttg tggctgtttt ggcaaacggc cttgtttcat caaatcatgg
     7681 atgacttggc gaacatatac gttgatttcc tgttggaccc tgaaacctaa gaacagattc
     7741 acttggacaa taaaatcagt gtccatcata tctacttgat attctttcgt atatggctca
     7801 tccgtcactt ccacatgaac aaaccaatag actcgtgctc ttttaggccg tttatctaaa
     7861 atcgaataga tgaactgtcg gccaacttta tcttcagcca tatcaggaac caagaagacc
     7921 acatttgtct gatagattgg taaagaagta tcatcacgta attctctaag ctgcgggata
     7981 taatctctca aagacacatc ctcggttgac gattcgcgaa taatgttccc ttgttcccaa
     8041 ataaacatca cgactaagat gatcaaacca atcagaacgg ctacaaaacc gcctcggaag
     8101 aatttgacaa cacttgagat aaagaaaacg acttctatcg caccgaagaa aaaggtaact
     8161 aaatatgcaa gccaacgaga atgcccattt tggataagat aaaagtaaag cagtgctgtt
     8221 gtcatcaaca tcgtcacagt gatcgataga ccgtaagcag cttccatatg atgagacgtt
     8281 cggaatgtga caaccactag agaacaggcg atccataaaa tcagattgac tgctgggata
     8341 tacatttgcc cgatactgtt cccaggatac atgatcttta accgaggcag cagtttcaac
     8401 ttgattgctt ctgaaactag cgtaaacgaa cctgtaatca atgcttgcga agcaatgact
     8461 gcagcgactg ttgcaaaagc aacgccgatc acgatccaac tttgaggcat catttggaag
     8521 aatggattca ggttcttgat ttgctgtgct tcagggtttt gataaacatt caacaaccac
     8581 gcagcctgcc caaaataatt gataaccaaa caaattttga cgtaaggcca gctcagacga
     8641 atatttcgtt tgccgacatg tccaagatcg gaataaagcg cctctgcacc tgtcgtagcc
     8701 aggaaaatat ttccaagaat gaacaagcct aatttgttat ctgcactaaa aagcagctgg
     8761 atagcataat aaggattcag tgcacggata actgtccagt cttgactgaa gttcattaat
     8821 cccattacgc ctaaaaaagt aaaccaagcg aacatgattg gtccgaatgc ttttccgaca
     8881 atatctgtac caaagcgttg gaccatgaat aaaacaaaaa taataactaa agtgataatg
     8941 acgatcacgt tctggttatt accgaaccgg tcaaaaaact gcggaattcc tcgcaagcct
     9001 tcaatcgcag tcgttaccgt cacagcaggg gtcaataccc catctgctag taaggcagct
     9061 cctccgatca ttgcaggaat gatcaaatat ttcccacctt ttcggacaag tgtgtagagt
     9121 gagaaaattc caccttcacc atggttatct gcattcaatg caatcacgac atatttgatt
     9181 gttgtcagta gagtcaatgt ccaaaaaatc aatgagactg atccgataac aaaatcagga
     9241 ttgattcctg acaatccgcc attatcttca acaatcgatt tcataacgta aagcggactt
     9301 gtcccgatgt ctccgtagac gactcccatc gctacaagca ggccagccat tgatatcttt
     9361 ttggtattgt gcgttgttgc cacgtttttc cctcatttct tgttatatgg atatccttta
     9421 ggtaagcttg aatgatagat gatattctta tccatgacac ccgctttcct aaattgcttc
     9481 taattatcca agtaacacac tgaagtgtca acttatttga ataaaattat tctttctttg
     9541 gacgttctct gatacgtctt gccacaaatg aataaagagc aaggcaaaaa attgcaccca
     9601 ataatgctgg tatcaaataa aaaccgccga gttccggtcc cagtggtcct agtaaaaatt
     9661 cgccgagcca actacctaaa aaaccgataa tgatatttcc agatacgcct cccgggactt
     9721 cctttcctaa aattgcgcca gctatcaaac ctaatatacc gccgataaat aacgaccata
     9781 aaaaattcag catctccttt ccctccttta tccatatttc tcacttttat tttacgcttt
     9841 attcagaagg actccaaaaa tatgcgccaa gaaactccag aaaaaaagaa gttgtaacaa
     9901 aagtgttcag cttatttccg tagaagctgc ttctggaaca ccgtgaatca gaggttgtga
     9961 aatcagcggt ttgacctgag tattagagaa acaaacggaa aaatagttcc tcatattttg
    10021 ccgttttttg agctaatatc gagggacagc tgattgaaca tcgtttattg gagaggttgt
    10081 aaaaaagctg ttctgcatca agtaataaga acagccaagt aaaaacagct cctaatgttt
    10141 ttaacttggc tggacttatt accgcagatg caagcttttg aacaccgttt attcgaaaaa
    10201 aaaggactgt gacacggctt ttgtcacagt cccctttttt cgaataatta ttgtccccct
    10261 gtcaccttca cgatcacaag tacattattc ctatgaatta aattattttt cttcattttt
    10321 cgttgaacca tcttcgttca acattgcttt tcttgacaga tttacgcgtc cttgtttatc
    10381 gatttcagtt actttcacaa ggatctcatc acctagttta acaacatctt cgactttatt
    10441 gacacgatcg tttgctaatt gggaaatatg gacaagtcca tcttttcctt tgatcaagtt
    10501 gacgaatgca ccgaattttt cgatacggac aactttccct agataaactt caccaacttt
    10561 gacttctttg gtcaaatctt cgatgatctt gatagctttt ttgatcattt ctgcatcagc
    10621 tgaagcaata cttacttttc catcttgatc gatatcgatt ttcacaccag tttcatcgat
    10681 gattccgttg atcgtatcgc cgcctttacc gataacatct ttgattttag caggttcgat
    10741 ctggatcatt tcgatttttg gtgcatattg gcttaattct tcacgtggag cagcaatcgt
    10801 tgaagtcaat tcttctaaga tttccatacg tgcttgtttt gcttgagtta atgcttcagt
    10861 taggatttgt tctgtgattc cttgaatctt gatatccatc tgtaaagcag tgataccatc
    10921 ttttgtaccg gcaactttaa agtccatatc gccaagatga tcttccagcc cttggatatc
    10981 agttaggatc gtatagtttt caccatcaga aacaagtccc atcgcaatac cggcgactgg
    11041 cgctttgatt ggtacacctg catccatcaa tgctaatgtg ccagcacaga tacttgcttg
    11101 ggaagaagaa ccatttgatt ccaatacttc tgctactaaa cggatcgtat aaggaaattc
    11161 ttcttctgaa gggattactt gtgctaacgc acgttcacct aatgcgccat gtccgatttc
    11221 ccgacgccct ggagagcctg cacgtcctgt agaaccaaca gaaaattgag ggaaattgta
    11281 gtggtggatg aatcgtttgc tgacttccac ccctaaacca tcaatgattt ggtgctctcc
    11341 tagaggtgcg agtgtacaag ctgataaagc ttgtgtttgt cctcgtgtga acaaacctga
    11401 tccatgtaca cgtggcaata agctgacctc tgatgacaat ggacggattt catccaattt
    11461 acgcccgtca ggacggatct tatcgatggt gatcaattca cgtacgacat ctttttcaag
    11521 atcttctgtg atttgtttaa cttctttcaa caagtgatct tcatcttcat gacctgcaaa
    11581 tttctcagca taagcttcgc ggatatcgat cttcacctgc tcgatattat cttcgcgagc
    11641 aagtttttct tcagtcatta ctgctgcttt cattgtagcg taagaagcat caaagatttc
    11701 ttttttcaat acttcatcaa cttgcagtaa agtcacttcc attttttctt tccctactgc
    11761 ttgaacgatt tcttcttgga aagcaactaa ctctttgata gcgtcaaaac caaacagcaa
    11821 tgcaccaagc atgtcttctt ctgaaacttc tttcgcgccg ctttcaacca tattgatggc
    11881 ttgtttcgtt ccggcaacag tcaattcgat atctgtttgt tctgcttgtt ctactgttgg
    11941 attcaagacg tactcgccat ttacacgacc aacttctacg cctgcgattg ggccgtcaaa
    12001 tgggatatct gagattgata gtgctaaaga agaacctagc attgctgcca tagctggcga
    12061 gcagtctgtt tctacactca tcacgatgtt cgttacttgg acttcattac ggaagccttc
    12121 tgcaaacatc gggcgaattg gacggtcgat caaacgggca gtcaatgttg cttccgtact
    12181 tggacggcct tctcgtttga tgaaacctcc agggattttc cctgctgcat acattttttc
    12241 ttcataattg atcgttaatg ggaaaaagtc tgtatctttt gcttctttag aggccacagc
    12301 tgcacttaag actactgtat ctccataacg gactaaaacg gctccgtttg cttgttttgc
    12361 tagctgaccg acttcgattt gtaagggacg gccgccccat gtcgttttga atacttgttt
    12421 ttctgacata acttctcctt ttccttctcg tgaaagcaag acgctactaa acaaatgaaa
    12481 actgccgaaa gccgtctttt gagcagcttt catttggtta gtaggagtcc atctagcttg
    12541 atcacggaat tttacttaat tgatatacga gtaaaaaaag tgagattcat gtgaatccca
    12601 ctctttttga ctaaaattaa cgacgcaagc ctaaacgttt gatcaattcg cggtaacgtt
    12661 gaacgtcagt tttacgtaag taagctaaca agttacggcg gtgaccaact tttttcatta
    12721 ggccgcggta tgaatgatgg tcttttttgt gcacgcgtgc atgttcattc aagtggttga
    12781 tttcttcagt taatacagcg atttgtactt ctggagaacc agtatcccct tcatggcgag
    12841 cgtattcgtt gatgatttca ttcttgcgtt cttttgaaat tgccatttct ttcacctctt
    12901 tcgattattc ccctgcgact gagtaaaacg ttggtgattc gcttaaccaa gtggcaggtc
    12961 atgtgcctag agcacacagc tatactttac ctgaaaccgg gttgaaaatc aagtagttaa
    13021 tcattaagaa aaggtagttt tcaaacaatc ttactactac ttttccattt ttgtggtaaa
    13081 ctataatgcg aaaatagact tgaaacacag tcttttaaga aaggggtaca tattccatga
    13141 ttacaatgga tgatatcatt cgtgaaggaa atccgacctt acgcgaagtt gcaaaagaag
    13201 tttctcttcc attaagcgaa gaagatatct ctttaggaaa agagatgctt gagttcttaa
    13261 aaaacagcca agatccaatc aaagctgaag aactacactt acgaggcggt gttggcttag
    13321 ctgctcctca attagatatt tctaaaagaa tcattgctgt tcacgtacca agtcctgacc
    13381 cagaggcaga tggaccttct ctaagtactg ttatgtataa tcctaaaatt ttgagccatt
    13441 ctgttcaaga tgcttgttta ggggaaggtg aaggctgttt atctgttgat cgagaagtac
    13501 caggatatgt tgttcgccat gccaaaatca ctgtgtctta ttatgatatg aatggtgaaa
    13561 aacacaagat tcgtttgaag aattatgaat cgatcgtcgt tcaacatgaa atcgaccata
    13621 tcaacggtgt gatgttctat gaccacatca acgatcaaaa tccatttgca ctaaaagaag
    13681 gcgtattggt gatcgagtag agaattggca ggttccttcg atactatgct acaagaaagc
    13741 tttataaaaa aggggagtgc tcaaaagctg cacttccctt ttttatgttt ctccactaat
    13801 cgaaatttag atagacatct agaaatcgca attccaaaaa attctgtgta ttggatacgc
    13861 atcttttctt attttttctt atttcttcca tatgggtgtt tatctcagag atgatagaat
    13921 agtttctagg ataaaaagta gcagctacga tgttatcctt cgtagaggat tcctgcttca
    13981 gctagttttt gtttgatgtt tttcagcata tgcttttctg ggtagcggat cgtatgcaga
    14041 tggatgccgc ctgttaattc ggataacaaa gtagcattac ttttttgata acgtctcata
    14101 aaggaatcga tatcttgtgg cgttttgatg tgcaaagttc ctgctaactc accgtaaagg
    14161 ggatgttcca caattacatc aatgatttca ccaccatttt caacgattag tctcagttct
    14221 tcttctgttt gatcttttcc atgctggaca gcgattttac tgatgtgccc agtattggtt
    14281 tcttctgttt cgaggatata acctcttgct gttgccacga tacttttacc tgcagctcgt
    14341 agtaaagcaa tatcgcctac aatgatctga cggctgactc caaaagtttt cgcaaacttg
    14401 ctagcactca ctggtttttc tgtatttgcc aaggtttgca tgatttgttt tcgccgtgtc
    14461 tctccttcga tcacacttat tcctccttca ttttgttttg gtattcgcgc gagtgtttca
    14521 tcaaatcaac atacgtgtct gttattgttt ccttatatcc cttatttttc agcagactaa
    14581 caactttatc caatacttgc tgctctcttt ttgcatcata aactgcttgg ttttcttctt
    14641 ttttcacttg tcctatttta gttaccgtat ccattctttt ttctaataaa gcaaccaact
    14701 ctcgatcgat catatcgatc ttatgacgta atgtttcaag agactcttct gttagttctt
    14761 cctctactgt cttgatgata cgagcaggat ttcctgcaat cactacattg tcgggaaaag
    14821 atttggtcac aaccgaacct gcgccgacta ctacgttgtc ccctagagtg acacccggcg
    14881 tgacgataac tcctccgcct aaccaaacat tattaccgat tttaatgggt tttgcgtact
    14941 ccagaccgct gttacgtttc actggatgta gtggatgcgt cgccgtgtaa agctggacat
    15001 tcggagcgaa catgcagtta tcaccgattt caatcgtgct gacatctaaa aatgtacaat
    15061 tgaaatttgc ataaaaattc tccccaacaa atatattata tccgtaatca aaattgataa
    15121 ctggctccat atagattttt ttccctgtac ggccaaaagt ctctttcaac aattgggatc
    15181 gcagttccgc tgattctgct tggttgatga tttggctttg acttcttgca aacatacggt
    15241 cggcaaccag ctcaggatca ttggcaaaat aaagctcgcc tgcaatcatt ttttcttttt
    15301 cggttttgaa ttcagtcata atctgctcct aattcttgta tttttttgaa aacaaccgct
    15361 ccgtcttctt gaataaacgt ataaggctcc gctaaataaa caggcgtttg attgattact
    15421 aagattttgg ctgacggctg gcgaaactgt accagatcac aaaacggatg aacttgaaaa
    15481 ctcgttccaa caatgacgat cagctctgct tggctgacgg cataagccgc ctgctccatg
    15541 actccatctt gaaacccttc accatataac acgatgtctg ggcgtatttg tcctccgcat
    15601 gcttgatgtt tgtcgctgtg aagatattct ttccagtcta ccgtttgttg acattttcga
    15661 caatgacatt gatacagact cccatgaaaa ttgacgagtt gcgggctttt agcctttgcg
    15721 tgcaaaccgt cgatgttttg ggaaacaacc caaacatttt tttctttcgc tagatgtgct
    15781 atttccaaat gaatgatatt gggttttgct tcaggatgat aaatcttttt gacaaattga
    15841 tagaatttat ccggttcatt caccattgct tgatgactca gcaagtattc cggctgctcc
    15901 atcccgtggt agattcctga caaagatcga tagtctggca ctccagaagg tgtggatacc
    15961 cctgcaccgg tcaaaaatgt aatttgctga taagctaata tctcttttgc tgcttcgtct
    16021 actgttaatt gtttcatgtt ttgtcacctc actttccatt atagcccata ctcgaacaaa
    16081 tttattgttg aaaagtcaga aaaatgggaa taaaaaagtg ttatgggcat ttgagcaatt
    16141 tatcacaatg ttcactgaca aaagaatgaa aacgtgttac aatactgctg tataaaagtg
    16201 aggtgaatcg atatgttacc attctttatt tattggggcg ttattctggc ttgtatcgcc
    16261 tggttagcgt taagcgttta tttctctgtt ttttatcttg tacgcaaaga aaatggaaat
    16321 ctttgggctt ttgctttatt caacgtgatt gccgcaattg ttttagccat tacattagcc
    16381 gtatatcgaa catggggctg gggaatcacc caatactcaa gtttgattta tctaatatta
    16441 gccatttatg gcgtcgttgt tcttttgcaa gcgatcctag gacgtgagcc aaagaaagcg
    16501 gcagcttaaa tgatcgagat ggaactggac acaggaaatt ttctgttcta gttctttttt
    16561 tattgtatta catacatttt tgtgtataat tatactatcg tttgcaatgg atagatgatt
    16621 cacattttga ttgttttttc ggtaaaagta aagatttcta tatttatcag tattttttca
    16681 aaaaccagca gttatagatt gacaaagcct ctcgagtatc gtaaactttt tttgtataaa
    16741 taactgaata cgagatgcct caatcaatga ggtagaggtc gcgagaatca ttaaccctgt
    16801 ggagtgaagc aaacatggag aagcaggaat agggaggatc gccgaaatgt gcactttttt
    16861 gcttggagtg tatgttggga cgcaagttaa gagcttgcgg actgtcttag cagtgatgct
    16921 aagttgcgct atgttttgtg agaggtttat caccttatgg aattttagcg gcctttttat
    16981 atctataaat cggtcgcttt ttattttttc agataaagct ttaccgcatt tcatctagac
    17041 cgctcgtgta tcatataatc agaaagggag aaaaaaatga aaaaaaatat ggtttcagtt
    17101 gccgtactat ttgcttttgt cttctcacta ttcagtgtgg gatctctggc aaatgcagat
    17161 gaaaaaacac cgagcgatga gtttcgcgta ggtatggagg ctgggtatgc acccttcaac
    17221 tggtctcaac aaacagatga gaataatgcc ttgccaattc aaggccaaaa tagttacgcc
    17281 ggcggttatg atgttcaaat cgctaaaaaa gtagcagatg gtttagggaa aaaattagtc
    17341 atcgtccaaa cgaaatggga tggtctggct cctgcccttc aatcaggcaa gatcgacgcg
    17401 atcatcgccg gaatgagccc tactgctgaa cgacgaaaag aaattgattt taccgatcct
    17461 tattacgaat cacaattagt agtagtagtt caaaaagata gcaaatatgc aaaagcaaaa
    17521 agtttagcag atctttctgg agcaaagatc actgctcagt taaatacatt ccactatact
    17581 gtgatcgatc agataccaga tgtgcagaaa caacaagcaa tggataattt ctcagcaatg
    17641 agaacagctt tagcttctgg aatgatcgat ggttatgtca gcgaacgtcc ggaaggtgta
    17701 acggcaacta gtgtgaacaa agacttggaa atgcttgaat tcagcggtgc agatggcttc
    17761 cagacaaacc cggaagatgt tcagatttct gttggtatgc gtaaaggcga cccggatatc
    17821 caaaaagtaa accagattct ttctggcatc tctaacgatg aacgaaccaa aattatggac
    17881 caagcagtca aagatcagcc tgctgcaact gatagtgaag aaaaacctgg catcatttct
    17941 gatttcacta cgatttggaa acaatacggc aatatgttct tacgaggtgc tggacttacc
    18001 ttgttcatcg cattgatcgg gacagttgtc ggtactacac ttggattact gattggtgtc
    18061 ttccgttcgg tacctgaatc aggaaatgca ctgactcgct tcttccaaaa aatcggaaat
    18121 gcacttcttt ctatctatgt cgaagtattc cgcggaacac cgatgatggt acaagcaatg
    18181 gtcatcttct acggtctggc actcgctttt ggcatttcat tgaaccgaac gattgccgct
    18241 ttgtttattg tctctctcaa tactggagct tacatgacag agatcgttcg tggggggatt
    18301 tttgcagtag atgaagggca gttcgaagct gcgcaagcca ttggtatgac ccatggccaa
    18361 acgatgagaa aagttgttat tccacaagtt ttacggaata tcttgccagc aacaggaaat
    18421 gagtttgtta tcaatatcaa agatacagca gttttaagtg taatcggcgt agctgatttg
    18481 ttcttccaag gaaactctgc ttccggtgcg aatttccaat tcttccaaac atttacgatc
    18541 gtcgggatca tctacttagt catgaccttt acgatcacca gaatcctgcg ggcggtagaa
    18601 aagaaaatcg atggtccttc agcttatacc aagcttgaag aaatcgacaa ttcaaatttg
    18661 caggaataag gaggaagaat catgaatgaa aaaattattg aaatcaaaca tctaagaaaa
    18721 agcttcggtg aaaatgaagt attgaaagat atcagcgtaa cagtcaataa aggtgaagtt
    18781 gtgacgatca tcggttcttc cggttctggt aaatctactt tcttgcgctg tatcaattta
    18841 ctggaaaaac caacaggcgg cgagatcatt tataatggag aaaatgtatt agctcctaaa
    18901 tataatttac ctaaatatcg aacgaatcta ggtatggttt tccaatcctt caacctgttc
    18961 aacaatatga atgtcttgga aaactgtatg tctggtcaaa caactgtcct gaaacgagat
    19021 aaagaaactg ccaaaaaagt agcaatcgaa aatctggaaa aagttggtat ggatcgttat
    19081 atcgaagcca aaccagcaca attatctggt ggacagaaac agcgtgtcgc aattgctcgg
    19141 gcattatcaa tgaatccaga tgtattgcta ttcgacgaac ctacttcggc gttagaccct
    19201 gaaatggttg gcgaagtgct taatacgatc aaagacttag cacatactgg cttgacaatg
    19261 cttatcgtga cgcatgagat ggaatttgca aaagatgtat ctgaccgtgt catttttatg
    19321 gataaaggtg tgattgcaga agaaggtaca ccagaagata tctttgtcca tccgaaagaa
    19381 gaacgtacaa aagaattcct ttcacgtatt ttaaatgctt aggatcaata gctgattggg
    19441 acaaagagct gcggtgttta ccgcagttct tttttatgaa ttactcataa aaggtaaatt
    19501 tctgaaaatt ttggaatctt agttgtgttt ttcagaaaac tctcttaata tggaagtaat
    19561 taaacttttt tagttaggat gatttaaaaa tgaatgaaga gcaagaactg cagcgtggac
    19621 tgaaaaatag acatgtccaa ctgatttcta ttggcggagc gattggaacg gggttgtttt
    19681 taggttccgg aaagtctatc cagctagctg gcccttcgat attgctcgcc tatcttatta
    19741 ctggatgcat ctgttttttc attatgcgtg cgttaggcga acttctgtta tcaaacacca
    19801 ataaccattc ttttctggat tttgttgcag aatatcttgg caaaaaagcg gctttcatca
    19861 ctggctggac gtattggttc tgctgggtat cgattgcaat ggccgattta acagccatcg
    19921 gaatgtatgt acgctactgg gcgcctggtg ttcctcagtg gcttcctgaa ctgattgcat
    19981 taggtcttct tctttgcttg aatatggtgg ctgtctcatt gtttggtgag ttagagtttt
    20041 ggtttgcatt gatcaaagtt gtagcgatca ttgcctttat cctcgtagga gcttatatga
    20101 tcctcaccca ctatacgaca gatattggca cagccagtat cacgaatcta tggtctcatg
    20161 gcggattctt tccaactggt gcaaaaggat ttatcttagc tttccaaatg gtaacctttg
    20221 cttttgctgg gatagaatta gttggattag tcgctggaga aacagaaaat cctgaaaaag
    20281 tgttgccgga agctatcaat aatattccta tcaggatcat cttattttac ttaggatctc
    20341 tttttgtcat tatggcaatc tatccatgga acagcttgaa tccagataac agtccatttg
    20401 tagaagtatt ttcggaaatt gggatcacga ttgctgcttc cttgatcaat ttagtcgtat
    20461 taagtgcagc agcttctgcc tgcaacagtg caatatatag tacaggacgt atgcttcgtt
    20521 ctttagcgca agaaggcagt gcccctaaaa aattcaaaaa gttaacaaca catcatgtgc
    20581 cagggaatgc tttgacattt tctactatcg tcattttcat ttctgttatc ttgaattacg
    20641 tgatgcctag tgaagtattc acactagtaa gcagtattgc cactacttgt ttcttgttta
    20701 tctggagtat gcttgtctac acgcacatga aatatcgtaa aagtatcctc ggcaaaaaag
    20761 ctcattcatt taaaatgccg ttgtacccat ttagtaatta tctggtattt ctttacatgg
    20821 cctttgtgtg tgtcgtcttg ttcctaggaa aagatacgag aatcgcttta ttactaacac
    20881 ctgtgtggtt cttgcttcta ttactaatct atcatatgaa atatgaacgt aagaagtaag
    20941 aaagtagttg tgacagaagt ggtcagcttc gagaaataag aaggaattta cgaaaattgc
    21001 ttcttaaatt tttgtgaaat ttcggcttat ttcttggaga taaaagcttc tgtcccagcc
    21061 tcttttctat ttccccggaa acaaccgctg ataataggca tctgcttttt tataggaaaa
    21121 aatccaaata ccagatacaa aaaaacgtcc gaataacccg atcaacggta caaaaaagac
    21181 accggcaatc aagcataagt taatcatcat ggagataaga cttcgacggt cctttgacaa
    21241 aactctcacg acatgggaat ctggtttatt tgctttcatg atttgcctca tcaagatacc
    21301 aaaagaaaaa ctccacatcg tgtatactaa gacataagcc atctcaggcc aaaaagcttc
    21361 agggttacgc ccgacccaac tagtaacaga aggacataaa gtcagccaaa agagcaaaaa
    21421 gatattcgac cagaccactc ggtaattaat tcgttctgcc atctgaaata tacgatgatg
    21481 gttcacccag atcaccgcaa caaatataaa gctgatggta taagctaata atgtatttcg
    21541 gatgacccat aaagaatgaa agctagatgt attagggatc ttgatctcta atacaataac
    21601 tctcaaaaca atggctacca ctccatcagt aaatgcttct aatctcgact ttgtcacgat
    21661 gattcctcct cagatgattc agcgctttta ttttatcact tacgttatta aataaccatc
    21721 gttttatata gggaattttc tgaaaattgt tgcatatttt tcgaaatttt gttactttta
    21781 aaaaaaccgt aaaggagtta tgtatgacaa aaaaattatc atttaaagac tatcttttta
    21841 taggctccat gcttttcggt ctattttttg gtgctggaaa tcttatcttt cccgttcatt
    21901 tgggtcaaga agcaggcgct gctacctttt gggcgaatct aggtttcttg gttaccggta
    21961 tcggtcttcc cttcttaggg gtgattgcca ttggtgtatc gaaaagctca ggtgtatatg
    22021 aattagcaaa acggataaat caaacatatg cgatggtttt taccatcttg ctttacttag
    22081 ttatcggacc gttttttgct ttacctcgcc ttgcaaccac atctttcgag attggtttag
    22141 caccatttat cgatcctagc agacagacgc tatatctagc aattttcagt attgtatttt
    22201 ttgtgctggc ttggtggttt tcaagaaaac cgacaaagat attagattat gttggaaagt
    22261 tcctgaatcc tgcatttttg gttttattag gtgctttgct tctccttgct tttttgaatc
    22321 cactaggttc tgtaaatcaa gcgcctattc agccaaatta tcaagagcat gcttttttca
    22381 cagggtttac tcaaggatat aacacattag atgcgcttgc tgctttagca tttgggatca
    22441 tcattgtcac aacaatccaa aacatgggtg taacaaaacc tgctgaaatc gctaaagaca
    22501 cgatcaaatc tggtgcgatc agcattatat tgatgggaat catttatact ttgctcgctt
    22561 atttaggtgc tatgagctta ggtagctttg ctctaagtga aaatggcggg atcacgctgg
    22621 ctcagattgc tgatcattat ttaggtactt atggcagtat cttattagca tttatcgtta
    22681 tattagcttg cttgaagacc ggtattggtt tgattacagc ttttgcagaa acattcacag
    22741 atttgtttcc gcaaagaaat tacgcattct ttgttgcact tgctagtatc cttccttgtt
    22801 tagcggcaaa cgtaggcttg acgaatatca ttcaattttc gttgcctgtt ttgatgttca
    22861 tctatccatt agcaatgaca ctgattttac tagtgttagt tggtcctttg tttaagcagc
    22921 gtacggctgt ttatcgaatg actacttatt ttacattgat tgcttcgatt tttgatgggt
    22981 tgaacgcttg cccagaaact atcaaacaaa cacctattgt tcaaaatctt ttatatgttg
    23041 cggaaagcta cttgccgttt ttcaaattgg ggatggggtg gatcgtacca gcagttatcg
    23101 gttttgttat cggactgatt tggagtttcg ctaagaaaga agaagtagca gattgataaa
    23161 agaggttgtg aaagagacgt tcagccttga gcaataaaaa gtaattttga aaaatggctt
    23221 ctcacatttt tgcataaatt tgacttaata ccgagaaact gacttttgaa caccgtttat
    23281 tgagaggtcg tgaaaaagcc gcttagtttc aagtattaag aaatattttg tgaaaatggc
    23341 ttctcacatt tttgcataaa tttgacttaa taccgagaaa ctggcttttg aacaccgttt
    23401 attgagcgtc tataaactga cctgagtatt agagaaacaa aacggcaaag tagctccata
    23461 tactttgccg ttttgttttg aactaatacc gaagagaagc tgatcaaaca acatttcttt
    23521 tataatatag attttagaca tatatatgat ataaaaatag ttttcgcaaa a
//
LOCUS       DACECY010000042        23432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.043, whole genome shotgun sequence.
ACCESSION   DACECY010000042 DACECY010000000
VERSION     DACECY010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.043"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(10..2154)
                     /locus_tag="IWO38_001798"
     CDS             complement(10..2154)
                     /locus_tag="IWO38_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0313886.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQTADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFEPVPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2511..3884)
                     /locus_tag="IWO38_001799"
     CDS             complement(2511..3884)
                     /locus_tag="IWO38_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0313887.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPIMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3884..4279)
                     /gene="rbsD"
                     /locus_tag="IWO38_001800"
     CDS             complement(3884..4279)
                     /gene="rbsD"
                     /locus_tag="IWO38_001800"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ0313888.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGSEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4307..5224)
                     /gene="rbsK"
                     /locus_tag="IWO38_001801"
     CDS             complement(4307..5224)
                     /gene="rbsK"
                     /locus_tag="IWO38_001801"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ0313889.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5333..6346)
                     /locus_tag="IWO38_001802"
     CDS             complement(5333..6346)
                     /locus_tag="IWO38_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0313890.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQISHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEESGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6427..7029)
                     /locus_tag="IWO38_001803"
     CDS             complement(6427..7029)
                     /locus_tag="IWO38_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0313891.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKGIVNNGTLSPHTAQSILTNN
                     DLLAKTLKRTAALKVLVNVDITTFSYLSHQTDAGGISALSAPAEFTDLLLKDSLKDTN
                     NMLTITKYNPSAFFGTSLDLQLRRRGIETIVLSGVATTNGVYATALDAFQHGYHVVLA
                     EDACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7214..7915
                     /locus_tag="IWO38_001804"
     CDS             7214..7915
                     /locus_tag="IWO38_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0313892.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7925..10636
                     /locus_tag="IWO38_001805"
     CDS             7925..10636
                     /locus_tag="IWO38_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ0313893.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEHNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKITEGKAQIAQGRTQ
                     LEDAKKQLAQQSAAIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKAAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWSYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11033..12661
                     /gene="treC"
                     /locus_tag="IWO38_001806"
     CDS             11033..12661
                     /gene="treC"
                     /locus_tag="IWO38_001806"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ0313894.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRITIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12721..13902)
                     /locus_tag="IWO38_001807"
     CDS             complement(12721..13902)
                     /locus_tag="IWO38_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0313895.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSVW
                     LVPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            14154..15680
                     /locus_tag="IWO38_001808"
     CDS             14154..15680
                     /locus_tag="IWO38_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ0313896.1"
                     /translation="MKRLIGILAMLVLAGMLTACGGASGKAEDSKEKLSVMTTFYPMY
                     DFTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQK
                     TLQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVAN
                     IRDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLAL
                     EYGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGV
                     SLEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNG
                     YFEDEDVQDRSLSDWAGTWQTVDTYVEDGTFAPVFEYKEKLNQDKTAQEYKEYYTKGY
                     QTDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGA
                     FRYIQFRDHTIAPADSEHFHLYAGNESQEALLKEMENWPTFYPDTMDGREIAQEMLLH
                     "
     gene            complement(15749..16477)
                     /locus_tag="IWO38_001809"
     CDS             complement(15749..16477)
                     /locus_tag="IWO38_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0313897.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(16490..17770)
                     /locus_tag="IWO38_001810"
     CDS             complement(16490..17770)
                     /locus_tag="IWO38_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0313898.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIALSAFLSALLAFCFKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYAQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLVVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(17911..18513)
                     /locus_tag="IWO38_001811"
     CDS             complement(17911..18513)
                     /locus_tag="IWO38_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ0313899.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            18759..18965
                     /locus_tag="IWO38_001812"
     CDS             18759..18965
                     /locus_tag="IWO38_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313900.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            19278..19997
                     /locus_tag="IWO38_001813"
     CDS             19278..19997
                     /locus_tag="IWO38_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0313901.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(20078..20761)
                     /locus_tag="IWO38_001814"
     CDS             complement(20078..20761)
                     /locus_tag="IWO38_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ0313902.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            20993..23179
                     /locus_tag="IWO38_001815"
     CDS             20993..23179
                     /locus_tag="IWO38_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0313903.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         7812 a         4210 c         4583 g         6827 t
ORIGIN      
        1 taatttattc tatttcttta aaggcagatc aagccagaat gatttgtcta aacttaacaa
       61 ggttagtaca ggaacagttg cagctttaac tgtacgagta gttgtttctg gtatctgtct
      121 tgccgtaaaa ttgttccaca aatcccagat tgaagcgaca cggaagatgg aagaagcgtt
      181 gttgacgcgt tttatcgttg aaaattctaa tcctgtgatt ggcacgccat ctgttgtcca
      241 tttgattggc acatcgaatc gcttagacat attgaggatt tcagcgtttg cttcttcagt
      301 tgttgctgct tcttttttcg gttcatcata ttttgtcagg tcgtacgctt cgatgatcgc
      361 gttcaatttt tgtgaatatt ggcgatctgt agcataacgt ccttccaaat atttggttgc
      421 ttctttgtag tcttttgttt ccgacttcca agttggtttg tagaaatcag gattgccagt
      481 cagtccattt ttgatcaagt cggcatagtc ttccaaactt tccttgtaag aaggatattt
      541 acggaattgt gcgcggattt ggtagctttg tccggaacta tcttgttcaa gtgtattgaa
      601 ggtcacgctg cttcctttat atgatccttt gataccaaaa aggttatgat ttggttcgct
      661 tgataataag ctgtttccag atccactttc aaggatagct tgagccatca tgactgatgc
      721 gtagattcct tctttttgcc cgatttcctg tgcatcatct gcaatctttt tcaagaactg
      781 atcagttgtt tggttttgac tgaaatggaa cgagccatct aatccatcta aaatagatga
      841 gccgcctgtc agcgaacctg aagcagcagg taaacctgct aagtttactc ggacagcaaa
      901 atcaggcgtg aaatctgaaa tagaagttac tcgaacttta tctcctggtt gaggcgcatg
      961 gataaattgt ccgttcccaa tatagatacc tacgtggtgg gtaacaccag aagaaggtcc
     1021 ccagaagacg agatcaccag gttgggcctg acttactgga atctgtgtac ctgcatattg
     1081 ctgttcgcct gtccagccac cgatataatg accggttgct ttcatatata cgtaataagt
     1141 caaaccagaa cagtcaaaag tatttggacc ttttgctccc catacatatg gttttccaag
     1201 atgcttgaat gcttctcgaa cgattgcttg ttggacattc gttgtatttg taggcaatac
     1261 agcatcaatt ggtgatttta caacaaaatc agaagaagca gaagatggat tctctatacc
     1321 gctttgattt gctgtatttg gacgagatga agcattgttc gttccgccat ttgatgaacc
     1381 attgtttgga tggtttggtt ctctctgagt atttccattg ttattcgatc cagatgggtt
     1441 tgtttgattc gaagaattcc cgctgttcga attattgtta ttattgtttg aattatttga
     1501 accattatta ttgttgttat tacccacatc tggtttgttt ggcgtttccg gatctggtgt
     1561 actagggact tcttcactag gatcagttgg tttctctgtt gaagtatccg gtacaggttc
     1621 aaattctgca gtaatagata aattgttcgt acctactgta aaggtctttg catcataatt
     1681 tacttctttg gcaccattaa cagcgactga agcaatttgt tcaccttcag ctggcgtaat
     1741 agtgtaagtt accttcgttt ccgctaataa accttctacc gttccacttt ccgtaatatc
     1801 caaaggttgc tgttcagggg aatccgctgt cgaagcagtc agttggatct taccaatcaa
     1861 agaaggagca attgtaaggg aatatgtctc ggctggcttt tcattttctg atggatcagc
     1921 cggatcaatt tcagttggct cggtcgatcc tgttgttgag ttagttgttc cagttgggtc
     1981 ttctgtaaaa gaatccgttg tttcactagt agctgagctg gatgtagtcg tatctgttgt
     2041 gactgtttga tcagctgttt gattatctac ggcaaatacg gtattggtcg acaaaacaga
     2101 cggagcaatc agccaagtag cagctagcaa acgtatcgtt tttttatttt tcataataaa
     2161 ctagtccttt tcctttcatt gattttctgt cttattcagc attcattaga cattttttgg
     2221 aacagataat cgtgtcgaaa agctttttct tttcataaat ctgtctgtgt attctaaata
     2281 aaacaatttg taaataagca acgagcattc attcgttttt cattgacgtt cattttattg
     2341 catagaccac ctttgtttgg ggataaacaa gtggggataa aaatggttat gcatatgtaa
     2401 attataccaa agtttggaaa gtatcacaaa aatttataaa aataatgatc ctaaaaagaa
     2461 aaagactgaa atatgtgata aacatctcat acttcagtct ttttcattaa ctattcataa
     2521 gacattaggt cttcatcaaa cccgctaacg agagtagtaa tcattgctac gactagacta
     2581 acagccagtc ctgctaagta ccagaaaaaa gtaccggaaa tgaatgcagg caatgtggtc
     2641 agactaccaa atacaaaagc gatcgctttc acttgggcaa atcccattac agctccacca
     2701 gcagctgcac caatgcaagc agcaacaaat ggttttttca atgggatcgc aattccgtaa
     2761 acagttggtt cggtcactcc cgcaagtaaa atcgaagcaa aagcagaacc ggagatcgaa
     2821 cgagtttttg aacgtttact tcgtagaaat acacctaaag ctgcaccagc cattccaaaa
     2881 ttggctgctc ctgtcaacgg aacgattcgg tcgaacccat aagcagagat attactgatc
     2941 atgatagggt ttacagccca ttggatacca aatattacca aaatattcca accaccagcg
     3001 atgattgcgc cgctgatcgg tccgttaata tccatcatcc aagtaattgc ggcagctaaa
     3061 ccttcgccaa ggtaaacacc cgcaggcccg aataccatta acgtcaaagg aaccgtgata
     3121 actaatgaaa gtaaaggtac aaatactaat tgcatatttt cgcgaatata ttttttgagg
     3181 aatttttcaa caaaagaata aaagaaaata cctaaaatcg ccggcatgac agtagacgta
     3241 tactgcatca gtactacagg aatccccaga aaatcagtag ctgctttgcc actgtctgaa
     3301 agtaaagagg tcagattcgg ttcaatcagt gctgcagcaa tgacagccgc aatatatcca
     3361 ttcactttaa acgttttggc ggcagaaaaa gcaagaaaga ttggcagaaa ataaaagacg
     3421 gcattcgacg cagcagacaa aatatgataa gttccgcttg tttcactaag ccagccaatc
     3481 gtagtcgcta aaacaaccag accttttaat ataccagaac cggcaaacag cggaatgatc
     3541 ggcgtaaaga tttccgatat caaaccaaat agtttttcga tcggatttag cttttcttta
     3601 ggagctgaat gatcgatttg acgcgttaat tccttgtaag caggagccaa tccctgatcc
     3661 atgattattt ctgcatatct atcgagtaaa atgaaatact taatggaagg gatttgctgt
     3721 tttaattttt ctgcattggc tactggctgt tttttcaatt tgatacgaag ccgagtcgca
     3781 catggaagga cagaatcaat attgtctttt cctcccaact gttcaatcag catatgaatc
     3841 tgctcggaca gtaccatctg ttcaggattc attgtcgttt ccattagaag accactccac
     3901 tcactaatat aatattggcg taaggtgtca tttcgcctgt acgaataaat gcatgacagt
     3961 tattcaactc ttttttcatt tcttcatgtg ggataaagct aatcggtgta tctggtaaca
     4021 ataatttgat tttttccaat atttccgggt tcattgtctt tatttcttcc gccaattcaa
     4081 tcgattgtac ctcaagttct tctaaaacgt tttctaaaac agataaaaag cttggaagac
     4141 cgtttgagac agccaaatct attttttcac taccaaaggg gacaggcatg cctgcatccc
     4201 cgatacttaa tttatcaaag tgccccattt gtgcgattac tcgtgaaata tctgaattga
     4261 ttacttttcc ttttttcatg tttgtttctt cctctcattg ttaattttag actgctgatt
     4321 gttctaaagc ttcgcggtaa gggatagaag gttgcgcacc ataagactgc acagcaacag
     4381 aagaggctag atttccataa aggatcgctt ctttcaagtt actaaggtct ggctgaagtg
     4441 tgctgcttaa tgcgccaata aatgtatcac ctgcggctgt tgtgtcaacg gcatccactt
     4501 taaaagcagg gacgataccg tgttcttttt ctgtatgata gaaagcgccg gcactgccta
     4561 gtgtaatgat tactgttcca atacctaatt catggagttt ttcggctgct gcgaccaaac
     4621 tgttatgatc tgtcactctg attcctgtaa tgatttctgt ttctgtttca tttggtataa
     4681 tgatatctgt tttttcaagt agttcagtag ggatcgtttc acgagctggt gcaggattca
     4741 ggatcgttgt ttttcctgcg tcttttgcaa tagaaaaagc taggatcgtc gcatctaagc
     4801 tactttcaaa ctgagcaata ataaagtcac tgtttgtaat cagttgcttg ttttttagga
     4861 cttcttgttc atggaaagca ttatttgcac ctgcatggat caagatacta ttttctcctg
     4921 ctgcgtctac cataatcatt gcttgtccgg ttgtggcgcc ttggatttca gctatcccag
     4981 atgtatcgat gttttctttt gtcagtagat ctaataactg ttgtccttcg ctgtcagccc
     5041 caacgcctcc aataaaagat gtccgtgcgc ctgaacgttt ggctgccact gcttgattgg
     5101 cacctttacc tccgccagcg tgaaaaatct catgcgcatg cattgtttcg cctggcttgg
     5161 gcatgttggt taaacgaagg gtagtgtcca tattaatact tccaattact gtaaccgtgt
     5221 tcattttaca aacctccaac tatctcattt attttgatgg gaaaaattat tttttaaaac
     5281 gttttagtaa aacgttttaa tagttcgata aaaatatagc acaccttgaa agctatttca
     5341 agggtgcagt tgaaaatctt ttttctaatc gtacaggtaa tcttttttct tcccattctt
     5401 tttctgggaa ttggatccga tctaacaata atttggcaga tgtttgtccc aattcgaaga
     5461 tcggttgtgc gatcgttgtc agtttcggct tgatatactc gcacatatct atattatcat
     5521 agccaataat gctgtagtct tcaggaatgc tttttccaga ttcctctaat ccgcgatata
     5581 atccaaaagc taattcatca tttaaagcaa acacaccagt tgcagcagaa tcaagaagtt
     5641 ctgctgtgat ttggtagcca ccttgttttg agaactgtgt aggtaagagg atcagttggt
     5701 cgtgagagat ttggtaaaca tcaagagcag atttaaaccc ctcaatccga gcgtggacat
     5761 tttccggcgg attttcagga tagacaaggg cgatcgtttg atgacctaga ctaagcaagt
     5821 gcatgcccgc taggtaacca ccgcgaaaat cgtctgttcg tacggcatca ctaaatcctt
     5881 cagatttttt ctgatccaaa acaatgaagg gccggccttg ttttttcaaa ttttcgttga
     5941 ttgcatctgt agagacagca gaagtcgcga taatgaatcc atccacacct cgacgtgtga
     6001 gctcggctag atattcgatt tctttttgat gatcagaatc agcattacat aatatcgtga
     6061 cgaaattctg cttatacaag atatcctcaa ttcctcgcat cagtgtactg aagaatggat
     6121 tcgtgatatc aggaacgagt acaccaattg ttttcgtttc cttggtaatc atttgtctag
     6181 caaaatagtc aggctgatag cctaattctt ctttggcagc taaaactttc tgaacagttt
     6241 tcggactgaa tttctccgta tttccattta atatcaagga gacagttgtg attgaaacgc
     6301 cggaaagttt agcgacttct ttgattgtga tctttttttt caccatgttc atactcctat
     6361 ctaaatcctg tggttatttt cccatgaaat caaaaagaag taaatgaaaa tcacgtgtgc
     6421 cagtacttat ttgctttgtt ggatagcttc gattatttgt ttcgttgagc gtactcgtgc
     6481 agttttcgga aatatctttt ttatgaacaa ttgatgggat tctttgtcac gatcactgca
     6541 agcatcttca gcaagtacaa catgataccc atgttggaag gcatctaatg cagtagcata
     6601 tacaccgttt gttgtcgcta caccgcttaa aacgatagtt tcgattccac gccggcgaag
     6661 ttgaagatca agagaagtac caaaaaaggc acttggattg tattttgtga tagtcaacat
     6721 attgtttgta tctttcaagg aatccttcaa caacaagtca gtgaattctg ccggagcaga
     6781 caaagcagat attcctccag catctgtctg atgacttaga taagagaaag tagtgatgtc
     6841 tacattcact aaaactttca aggcagcggt acgtttcaat gtttttgcta acaaatcatt
     6901 gtttgtcaga atagattgtg cagtgtgtgg agagagtgta ccgttattta cgatcccttt
     6961 ttgcatgtcg atcgcgacaa aagcagtttt tgataaatca aaaatcatat tttcctttaa
     7021 atcattcatg atgttccttc tttcaaataa caatgttttt tatgtaagta aatcataaat
     7081 cgtttatttg tcaactaaat aaaggattcc tttctttagt ctagagaaat tcagtggtaa
     7141 aataaataag tatgagaaaa cgcattcgat gcatagatat agcgagaaat atcgtgaaaa
     7201 tggaggaatc agagtgaagt atgtcgaagt aaaaaatgaa tacaaacgat acaaaatggg
     7261 agaaacgacc attacagcaa atgacgatat ttctttttca attgaaaaag gcgaattagt
     7321 gattatactt ggtccaagtg gagcaggaaa gtcaacgatt cttaatattt taggtgggat
     7381 ggatacacct gatgaaggtc agatcattat tgatgacaca gatattgcgc aattttcaga
     7441 taaacagctt actgcttacc gacgaacaga tgtagggttc gttttccaat tctacaatct
     7501 cgttcctaat ctaacagcaa aagaaaatgt ggaattggca acggaagttt ctcctgatgc
     7561 gcttgatcct gtagaggtat tgaggcaagt gggcctagct catcgattga ataactttcc
     7621 gtcccaatta tcaggaggcg aacagcagag ggtttcaatt gctcgtgcac tagctaaaaa
     7681 tcccaaactt cttttatgtg atgaaccgac aggcgcgctg gattttgaaa cgggcaagca
     7741 agtattgaaa ttattgcaaa acgctagcag acaacacggc aataccgttt tgatcattac
     7801 acataattct gctttagcgc ccatcgctga tagagtcatt cacattaatg atgctaaagt
     7861 aaggtctgtt gaattaaatg atcatccttc atccattgat gagattgtat ggtaggggga
     7921 ttttatgaaa acaagaacat atcttaaagc aggttttcgc gaaattcggc aatctaaagg
     7981 acgattcatt gcaatcatcc ttattgtcat gctaggaaca cttttatttg taggtgtcaa
     8041 aactgctgga cctgtcatgc aaaaaacgat ggaccactat gttggtacag caggactttc
     8101 tgatttgcaa atcgtttcta ctggtggctt gacacaaaaa gatattgctc aagcagaaaa
     8161 gatcccagat gcaaaagtag aaacaggaaa acagatctat tattccaata ctggtaaaaa
     8221 cgaagtgatt caaatatttt cttataacaa aaataacaag cagaacaagc tacaattaac
     8281 tgatggaaaa ctcccggaaa aaccaaacca gcttgtattg gatgaaaaag cggaagatga
     8341 agggtataaa atcggtgata tttatcagat tgattcagat gaattgaaag aaaaagagta
     8401 taccatcacg ggctttgtcc gttctccatt gttcatcaac aatttggaga gaggatacgc
     8461 gaacgtcgga aatggtagcg tcgattattt tgtctattta cctgaatccg tttttaaaac
     8521 agatatttat tctgtcttat atcttgattt tacaaacgta aagaatttga atacgtacag
     8581 caaagcttac aaagataaaa tggaacacaa tcaagagaag gcagaaaagt atttacaaga
     8641 tcgccctgaa gaacgcttag aagagctgaa aaaatcggca aacgaagaat tagaaccagc
     8701 acagcaaaaa ataacagaag gcaaagcaca aatagcacag ggaagaacac agttagaaga
     8761 tgctaaaaaa caattagcgc aacagtcagc agctatccag cagcttcctg aagcgcaacg
     8821 aacaatcgct gttcaaacat tatctcaaca agaagctcaa cttgctgaac aagaaaggca
     8881 attagttgag aaagaacgtg aactggcctc tgcagaggaa gaattgaatc gaaatgcaga
     8941 aaaaattgaa aatttaacta aaccatcata cctatacaat gaacgttcag aaaatgtcgg
     9001 ttttcaagaa tttggtgatc tgtctgatcg tatcgctgcg attgccaatg tttttcctgt
     9061 tttctttttc tttattgccg cattgatcac ctttacgaca atgacacgta tggtagaaga
     9121 aaaccgcagg gaaatcggga cattaaaggc gttaggttat acgaaagttg aaatcgcaaa
     9181 aaaatatgca atctatgcaa gtctagcctc agggataggc attgttttag gaacgatatt
     9241 aggaacgaat cttttgccaa ggatcatttt tgagctgtct aacgagcggt atgatattgg
     9301 gagtgcgctt attttttatg actggccgcc gattttacaa gcagctgccg cattcttcat
     9361 tgccgcattt ggcgctgcaa tgatcgtttt attcaaagat ttaagagaac ggcctgctgc
     9421 attgcttcaa ccgaaagcac cgaagccagg gaaacgcatt tttcttgaat acatcactcc
     9481 gctgtggtca cgccttagct ttaatcaaaa aatcagttac cgaaatctct tccgatataa
     9541 gtcaagaatg cttatggcga ttatcggaat tgctggatgt gccgggctga tggttgcagg
     9601 cgtgggattg aaagattctc taagttctgt ctcagctaaa caatttggac cgatcattga
     9661 ctatcaagca atcgttacat tggattcatc cacggaagat agtcgtcaaa aagcagcgga
     9721 tatgctgcag aagcaagaaa aagtaaatga gatcctagct attcatactc aaaccgtaga
     9781 attgagaaaa aaaggacatg cttcgcaaag tctgacagtg atcgtaccaa gtaaaacagc
     9841 tgatttggat cgttttatcc atttaactga tcttgaagag aacccacgct ccttgcctaa
     9901 aacaggaatc gctattacgc aaaaagcagc agaactattt gacttatcta caggagatca
     9961 tatttcgctt tatgatgaag atcaaaaaga atggaaagtc gaagtaaaag aaatcgtgaa
    10021 aaactatcta ggtagcttta tttatatgtc ccctgaatat tatgcttcag ttactgatca
    10081 atcgatgatg gataatgcat ttttgctgcg tacagataag atgacaacaa aagaagaaga
    10141 aatgctttcc gagaaactgc ttgccacaga cgctgtgaca aatacgtctt tcatgtccaa
    10201 acaaatcgaa acgcaagaag aatcaatggc gaacttagac tcggttgtct taatctttgt
    10261 cgtattatca ggactgctgg cttttatcgt cttgtataat ctaactaata tcaatatttc
    10321 cgagcgtgta cgcgaattat ctacaataaa agtccttgga ttttttgata aagaagtcac
    10381 gatgtacatt gttcgagaaa atatcatctt tactctttta ggaatcatcg gaggatttgg
    10441 tgtaggatat atattgactg acttcatctt gcaacaagcg tctatggaga atgtcatctt
    10501 tccattagtg atcacatggt ggtcttatgt cttatcagca ggattaacta tcgtatttac
    10561 ggttattgtg atgattgtta cccatttcaa attaaagcat atcgatatga ttgatgcgtt
    10621 gaaatcgaat gaataaaaac agaggttgtg aaatcagcgt tttgagctaa taacgagggt
    10681 cagctgctaa ttgaacaccg tgaatcagag gttgtgaaaa agctgtcctg catcgagcaa
    10741 aaggaatact caaataaaat tagctcctca tgttttacat ttgagaagac tgattgccgt
    10801 agataaaagc ttttgagcac catttataga agaaacaaga atattttgct attttaaaca
    10861 agaggataga caaatacatg tctgatctct tgttttttat tgggcaacag acctcttaga
    10921 atgtataaag ataaatttta caaacgcttt ctgttcagtt ataataataa atgataactt
    10981 gtatatataa gttgcggtag gtaataaatc agttgaatgg aggtaaatat ccatgacatt
    11041 taaagacaaa gtcatttacc aaatctatcc caaatcttac aaagacagta atggtgatgg
    11101 aattggagat ttaaaaggaa tcaaagaaaa actcccgtat ctgaatgatc taggcatcga
    11161 catgatttgg ctaaacccta tttatccaag ccctcaaaaa gacaacggtt atgatatctc
    11221 tgattacaca gccatcgatc ctatctttgg tacgatggag gaattcgaac agctaatagc
    11281 cgaagcaaag aaataccgga tagaaatcat gctcgatatg gtgctgaatc acgtttcgac
    11341 agaacatgaa tggttcaaaa aagcactagc aggggaccag cattatcagg acttcttcat
    11401 tttaagagaa gagccaacgg attgggtatc aaaatttggt ggaagtgctt ggtcgccatt
    11461 tggagatacg gggaaatatt atttgcactt atatgatcgg acacaagctg acttgaactg
    11521 gcggaaccaa gcagtaagag aggaactatt caaagttgtt cgtttttgga tggaaaaagg
    11581 ggtaaaagga ttccgtttcg atgttatcaa tgttatcggt aaagacgaac aattgaaaaa
    11641 taacaaagaa aatgatggca aacctgaata tacggacaaa ccaatcaccc atacgtatct
    11701 aaaagaattg aaccaagcaa cgtttgggac ggattccact atcatcacag tcggtgaaat
    11761 gagctctacg acgatcgaaa attgtatctt gtatacagaa cctagtagag aagaattatc
    11821 aatggtcttt aatttccatc atttgaaagt cgattatgaa aatgggaata aatggtctac
    11881 tcctccattt gattttgcag aattaaagcg actatttcat acatggggag aaaagatgag
    11941 tgagggtaac ggctggaatg ctctattttt taataatcat gatcagccac gtgcgctcaa
    12001 tcgatttgta gctatcgatc agtatcgaaa acaaggagca gagatgctag caaccatgat
    12061 ccatttgaac cgtgggacgc cctatattta tatgggagaa gaaatcggca tgatcgaccc
    12121 agactattca tcgatttccg actatattga cgtagaaagt ttgaatgctt ataagttact
    12181 ccttgaacaa ggattcacag aaaaagaagc gtttcgtcgg atcaaagcaa aatctcgcga
    12241 taactctcga acacccatgc aatggtcaga cacagaacaa gcaggattta ctacagggaa
    12301 accttggtta aaagttggag gcaagctaga agagattaat gtagaaaaag aactatcttc
    12361 tgaagattct attttgtcct attacaagaa attgattcgg ctgagaaaaa catatccaat
    12421 cgttgcacaa ggagattatc atgcttatga agcaaatcat ccggaagttt atggctttct
    12481 tcgccaattt gaaggacagc agctgcttgt attaacaaat ttctatgata aagatacaag
    12541 gatcaccatc ccagaaggat tcacagaagc caatattctt atcgataatt attccgatac
    12601 tgttcttcat caaagtctgg agctaaaacc ataccaggca attgctttgc tgaaaaatta
    12661 aaacaaaaac acaaaacgtc gttcctacga gctattctcg taagatcgac gttttgtgtt
    12721 ttatttaaaa taatcttctt cagaaaatga agagttttca tactctcctt tatcgccata
    12781 aaatttattg tagcgttgtt tgaatttacg gaaaataaaa gtaacgagta atttgactaa
    12841 cgtatagata ggaataccaa aaataacgcc cacaattccc attagatcac ccatgacaag
    12901 taaaacgatt aagatcgtaa tcggatggat ctgtaaacga tcacccatta ctcgaggaat
    12961 caccaagtca ccgtggagaa gttgtacggc aaaccagaca atgacaaatt tgacagccat
    13021 aaaagtagag tcttggaatg caatgatcaa acctggaaag aaggcgataa acggcccaat
    13081 atatggaatg atacataaca acccagcaga aatagctaaa acactagcaa attctaatcc
    13141 gatcagtaag aaacaaaccc agtagactgc tccaaggata aatgaagcaa tgatttgccc
    13201 ctttagaaat gaacccaatt gttgattagc aatctttgtc aaatttttaa aatctgtacg
    13261 aaatgctggt gggacaatcg ctaaaactga ctgataaaat ttttgaggat ctctcaaaag
    13321 aaaaaaagcg acaattggac cagtaaataa agtaataaaa atagctgtaa cagttgaaaa
    13381 aatgttgctg atccgttgtg cgccactttc ccaataattt ccaataaatc cagctatatc
    13441 agacgtcacg tcgtcaatag aagcgaaaaa ttgattaaat gaatcggcaa aaggtgtttt
    13501 ctctaaaaat tgtcggagac tctcttgaaa atcttgaaac aaatctggca aactactgat
    13561 caatgtcttt gcctgttctt caagaaaagg aacaagccat acgcttccaa gccccaataa
    13621 aaataaaata atcacataaa cgataatgac tgacaagttc ctcgaaaggt gcttgctcaa
    13681 acgtttgatg actggttcca aaagataata caagatcagt gcaaacaata atgggggcaa
    13741 gatagtagcg ataatgattc cgacaggctt gaatataaac gagatctgat ggaaaaaata
    13801 aatgatcagc gcaagtaaga taactattcc taaaacatag taagaggttt ttcctcctaa
    13861 aaagcggatc aaacgattcg ttgtatctgt ttcttttttc actctttttc ctcctttatc
    13921 tataacttca ttatagacaa aagcgggtat aaaaggaaaa tgatagaggt tgattgcaaa
    13981 aatatcgtca atcattcata taaactttct catcaattaa aataagaaaa cgcttgacag
    14041 ttcttttaac catatgataa attaaatcct gttgctaaaa cgtaactatt acgttttgta
    14101 aaaggcgaat cggaacattc aaatgaaaga agatgagaag aggagtgtca taaatgaaac
    14161 gattgatagg tatattggcc atgctggttt tagcaggtat gttaactgct tgcggcgggg
    14221 caagtgggaa agccgaggac agtaaagaaa aattgagcgt gatgacgacc ttttatccta
    14281 tgtatgattt tacaaaagca atcgtcgggg atgaaggaga agtagagtta ttgattccag
    14341 cagggactga ttcccacgat tatgaacctt ctgctaaaga catggcaaag atccaagata
    14401 cagatatctt tgtttataat gatgaaaaca tggaaacatg ggtccccgca atccaaaaaa
    14461 cattacaaga aggcaatgtc catacgataa aagcgacaga aggtatgctt ttacttccgg
    14521 gaagtgaaga aggacatgat catgaccatg aacacgggga agaaggccat acacatgagc
    14581 tagacccaca tgtatggcta gctccaagtt tagcaatcaa gcaggtagca aacatacgtg
    14641 atcagctgat tgaagcttac ccagaaaaac aggaagtatg gacaaaaaat gcagcagcct
    14701 ataccgagaa acttcaagca ctccatcagc tttatcaaga aacctttaag caagcaaaac
    14761 aaagatcttt tgtcacgcaa catgctgcat tcaactatct ggcattagaa tatggattga
    14821 atcaagtatc gattgctggt ctttcttcta gtgaagaacc gtctgcggcg agaattgctg
    14881 aactgaaaca ctttgttaaa gaacatggaa tcaactatat ttattttgaa gaaaacgcca
    14941 aagatagtat tgccagaacg ttagcaaacg aagcaggtgt atctttagaa gtattgaatc
    15001 ctttggaagg attgacgaat gaacaaatag aaaatggaga aaactatctt tctatcatgg
    15061 aagcaaacct cgaagcgttg aaaaaaacga cagagacaga aaacccgcta gaagatactt
    15121 tgacccccaa aaaagaaaag agcgtctaca acggttattt tgaagacgag gatgtacaag
    15181 atcgctctct ttcagattgg gcaggaactt ggcaaacagt agatacgtat gtagaagatg
    15241 ggacttttgc tcctgttttt gaatataaag aaaaattgaa tcaggataag acagcacaag
    15301 aatataaaga atattatacg aagggctatc aaacagatat tgcttcaatc aagatcagtg
    15361 agaaagagat ggcatttacg tttaaggatg gaacaacgaa acgttcaaat tatcgttatg
    15421 tagggaaaga aatcttgacc tacgaggcag gaaaccgtgg cgtaaggtat ctattcgaag
    15481 cagaagataa ggaaagtggc gcttttagat atattcaatt ccgtgatcac acgattgcac
    15541 cggctgactc cgaacatttt catttatatg caggaaatga aagtcaagaa gcattgctga
    15601 aagaaatgga gaattggcca acattttatc cagatacaat ggatggacgt gaaattgcac
    15661 aggaaatgct tttgcactga taggcaaaaa agaaccgaag ggtttggatc ggctagtcat
    15721 ttagtcggtc caaactattc ggttcttttt atgaaatcaa tttctttaat ccacggatag
    15781 aggctgggat ggtctcgccg tttgccaacg tgacttctct ggttttttta ttaatcgacc
    15841 gcacattttg tgtattgata acataggatt tatgacaacg gataaaattc cgatgcattt
    15901 tctcgatttc tttgatcttc tcatagaatt gtaattgggc atgagtcaaa tggacttcca
    15961 gtttatgtgg tgctcctagc gaagtagaaa aatattggat gtctttgatc ggtaaaaatt
    16021 tgatctcatc attaaactct attttgaaga tatctttttg gatctgggac atttgaaaat
    16081 cttcgaaaat tttgctcagg atatgctcga cattttcttt taaatcataa agatcttctt
    16141 tgacgatata atcagtcggt tcgatgttgc tttttaagat tttcatcgac atttccgtat
    16201 gggaagtcac atagattatt tttcctaagg gatctaattc acggatcaat ttaccgatcg
    16261 ctacaccatc catttcactg tcttctagtt cgatatctaa aaagtaaata ccaaaagaag
    16321 aagttctgat cttggctgca ttgagaagtt cttccggatc agaggtagaa atatggacat
    16381 aactgtctaa gttttcaatc atgattctat ttttgatcac cgttgataaa acttctaatt
    16441 gttttggatc gtcttcacaa atataaatgg gaatcatatc attactcctt tattttattt
    16501 gtatataaat cgtttgagaa aaacgctggt tttcaattga tgtttcaagg aaaagataag
    16561 gattggtctt tgtcaattca tccaagataa acaagccaag gccttcgttt tttggatttg
    16621 tttttgttga gaaccctttt tgtttcaact ggtttaacgg caatgtattt gttgttgtat
    16681 tgatcacacg aatcatcacg gaatgattct tcttgagtat ggtcaattcg atttttttct
    16741 tctttgcagc actagctcct tcaatcgcat tgtccaagat aataccaagc atacgtacaa
    16801 ggtttaccgt atgtttcgta tccagttcga ttggctcagg aaaatgaagc tggacgacca
    16861 accctttttc ctgtgccatc atgagtttta atgacagtaa actacggatc tcaggaactt
    16921 tcatattatc caacatactt agattggccg gaaaaaggtt catcatctgg cgtgtaggta
    16981 atatcgtttg agcataataa gaccgtaatc cttctaagtc gccttctgaa atatagctgt
    17041 gaagagaaaa aagcagattt tggtaatcat gtttgaattc tctcaagtct tgatagtttt
    17101 tttctagatc tttgatataa tcctgttgca tcctgacttg ttgctctaat aactgatcag
    17161 ctaatttctt attagagatt ttccaataat aaaccaagat gatcaggcca acacacagat
    17221 atagaatgaa aaaactcgta ttcaaagcta agacatcagg tgtttcacca gaaaaacggg
    17281 tataaaaaat aatgatatag tatgtaaata atatcaatgt cccgatcgta ccgatcatac
    17341 ggcggtccac agtgtgaaac caagtctcta acgatttttt aaaacaaaaa gctaataaag
    17401 cagataaaaa agcggataag gcaatgtgaa aatactgcaa tgaatctcct ggttcaataa
    17461 ccgaagattt caaaacaaaa aaatcgccga tgcttgttaa gtgatctcct aaaagcgacg
    17521 tcaataaccc aaaaggaagc gcgtaaacca attgttgacg cgaagtcgaa aagaagaaaa
    17581 aagtaagcat cagcattcct atagcaatta gagcagtaaa aatccctaaa atcgaataga
    17641 cccatccaat caaaatacaa gaaatcagaa tgactcccgc aaacagatat ttgtatcgga
    17701 tgtgatggtc aatcaataag atacaaattg tgtaaatcaa atattgcaac gctatggacc
    17761 ataaaaacat aaagtacagc tcccattgat ttttaccaaa ttatagcaca attagcgtat
    17821 tattttttta atttttcagg gattttgaat tcagaactca taccaaagca gcagccagat
    17881 acaaaaaagt cagataagtt agttaattgt ttaatgattg tttttttcat gaaagattcc
    17941 tccaaaattc atcaagtagt ttgtaatagg aagcaacgta gctatttcta aaacaatccc
    18001 aagtagcaaa taggtagaaa agtaagaagc tgagaaaaat aaagtaatga gagaaaaaat
    18061 tagaaacatg ctgatcgcct tctttaataa tgggggacgt ttctcgggtt caatgggatt
    18121 gattgctgta cctcttgatc catagcgaag cagtaacaaa aagcagctga ttaacaaaaa
    18181 gaccatcatt gggaaggaga tagtcaattg cagatgtttc atcaccaagg gaacgcccca
    18241 taaataaagt agtgtccaca gcagacaaac gaattcagag tcggaatgca atccataagc
    18301 atatctgcgg atgcatatat aagaaagaaa ggtgattcct gtttccgccc atgagcctgt
    18361 caccagagaa aagactagaa cgaccgctag ttttccaaga ttattcaata aaacttctaa
    18421 ggcaaatttt acttggacat acaccatatc ttcttccgat ttgtctccgc tgaataaggc
    18481 ttctaataat ttgttcacaa ctttatcttt catctcatac gtacctctac cacaaatttt
    18541 atgctaaaaa aaataaaaaa cgtcttttgt ttcataaaaa gtggttttga catcaaaacc
    18601 gtcatgattt cctaaaaaaa tcttcgtttt ccaagaacct taccaaaaaa agtgacagtt
    18661 ttgatacgaa aagtactttt tatgacaagt ttgtgctttt tcgagtgatt tatgttaaaa
    18721 aaattactga ccttataaaa ggtgatggag ggattcttat ggaaaatgaa caattgtttg
    18781 aagtcggcga aacatatgca tttaaatctg atttatttga atcaccaata aaaggaaaaa
    18841 tagaaaaaat ctatgaaaat agtgtgatga tacgtgtcat gaaatgcgca gtagaagaca
    18901 atgataccgc tcataatctg cactggcagt tagttgtacg caaatcaagt acaatagaag
    18961 aataaaaaca gataaaatgt tgagagcata aagaggttgt gagtctccgt ttagctctga
    19021 gttacaagga ataatgttaa aaaacagctt ttcatgtttt atgaatattg ccacttgtga
    19081 ccgaggtgtt ggcaggagaa tactgtttat ttgaaaaaag ggctgcgaca agacttttgt
    19141 cacagccttt attttcatga ataggagtta cttttggcta cttttagaga agtagctttt
    19201 aaggagcaga taaagtcaag gcttttattt ctataaatga agtagtaaaa tgaagaacag
    19261 attttattac aggaaggatg ccgataattg tgatcattgt tgcaggaacg atcggtgcag
    19321 ggaaaagcac actgactgaa atgcttgccc aagatttaga aacaaaacct ttttatgaga
    19381 acgtggaaga taatgaagta ttgccgctat tttattctaa tccagaaaag tatacttttt
    19441 tattacagat tttctttttg aacaaacgct ttttggcaat caaagatgct ttcagccatg
    19501 atgacaatgt actagaccgc tccatctacg aagatagtat gctgtttcat ttgaatgcag
    19561 atttagggcg tgtttctgaa gtagaagtaa agcagtatga aggtctttta gaaacgatgc
    19621 tgaaggagtt agaagaaatt tcgccacaaa agaaaccaga tctactggtt tatattcgtg
    19681 tatcttttga aactatgtta gcgagaatca aaaaaagagg ccgggaatat gaacagctag
    19741 aacaagatcc tgaactctat tcctactata aagaactcaa tcgacgatac gaagagtggt
    19801 atgaacagtt tgatatatgt cccaaaatag tgatcgatgg ggacaaatat gatttcgtag
    19861 ccgatccggc atgtggagaa caaatcgtac aagaaatcaa gatgcgggca aagaaaatga
    19921 cagaagaggc agatgcccag tcgatccgct acactacaaa taacccgaaa gcaaagggaa
    19981 tctttacaac taaataatat aaaaactaag caaattgctc actcgcagat atttctctgt
    20041 gagcagcatc ttgcttagtt tttttgattc attgagatca aatagtgaac cgattcgtat
    20101 gataaaattg ccataaatga aatgtctgag tgagggccat gatcaaaaat aaaatggaaa
    20161 cgatcaaaat cgaatgggcg ccaaacagat gatgacagaa aacaaggaca atagctcctg
    20221 cgataaaggc aagaacgaca cttagatagt ggaaaaatcg atcaaaatct gctttttctt
    20281 tagaaataca ataatcatat aagttcgttg ctaccccttt taaatttcca gttgtaaata
    20341 aattagtgta ggcacgcccg ttgattttat caaaagctac ccactggatc gatgcagtaa
    20401 aagcaatcaa aatcgttact aatgcgggat gctcatggac gggtgtcaaa ctaacaaaga
    20461 aaaaaatcac tgcttcataa tatagaacaa acaagcgcca gaaatgtttt tctttttttc
    20521 taaaataatc aattagatac tttgttagaa gaatccctaa gaaaaagaaa agggtagaca
    20581 atactttttt accaacactt agccattctc cgtcaaatgc ttggatgatc gatagaatga
    20641 tattgccagt ttgagcagag gcaaaagcac catattggat ataagtataa ctgtccatgg
    20701 ctcctccgat aaatgtcagc aaaagtccta ttgtacgccc ctcgtgaaag gaagtctgca
    20761 tttttttcat caccttttat gaatattttt gtcttcctta tcttagcata aacgattgaa
    20821 aaaaaggaaa aattttcaga aaactaaaat catatcgctt cccattttca tttttagaaa
    20881 ggtctgttag tataaagaaa aaatagacaa cgaaacttta aataaccaac ttgaaataac
    20941 caacttgaaa taaacaaatt caaagaagaa agatgggaag gaaatcaatc agttgaatga
    21001 aaaagaaatt ctccgattac aaaaagaatt gaaacgctgg gagaacaagt ataaaaagct
    21061 tcaacaagtc tttcggctac aagaagaaat cattgctacc tatgaagcgg cttatggcga
    21121 agcacccgga cttattgagg aagaagtaca agtaaaagaa agtgaaggaa acagctcttt
    21181 ctttaaaatg atggagaaac gttcctataa tcgcttcaat caagaacaaa aagcagctat
    21241 cacatatgat atgaagaaac atcttcgaat catagcagga gctgggagcg gcaaaacgca
    21301 aacaatctgt gcaaaagctg tttatctgat gacgcaaaaa caagtagacg aagaacgcat
    21361 actaatgatc acttttacaa ggaacgctgc aaatgaacta aaaaaacgag tagataattt
    21421 cagccagaga aaaaccgctg tccatatcgg aacattccat agtatctttt ttaggatata
    21481 caatgaaatt tgtcgaaaat ttcctgaagt agcaatgcaa ggaatacaag gagacttttc
    21541 aaaggatact gcacaaaaag tgaatgctgt tttgcaacag ctgatacgta aatataatct
    21601 ttatatattt gatcaatatg gagaaaaaac gattgcttct cggttagact attggcagaa
    21661 tatgaacctt tcagtagaag aaatggttca gttagttaaa gaaaaatatg attccattga
    21721 taagaatgca cgacagccga tatcagaacg actgtatgga ttgctcacgg aactccaaga
    21781 acaaaaaaga agccagcaat tgctagagtt caatgatgta ctccaaaatc tgaaaagcgc
    21841 tttggagaat gaggagatcc agcattatat cggccaaaaa tatgattatc tattcattga
    21901 tgaatttcaa gatactaatc cacttcaatg ggaaatcgtg aaacggatga caaaggaaaa
    21961 caggatcaag ttgattgttg ttggagatga tgatcagagt atctatggat tcagaggatc
    22021 agagccagca tacatcaaga actttgaaaa agaatatcct accaaaactc ttttcttatt
    22081 aactaattat cgttctcggg cagctattgt ccaaggagca aatagattga tcagctataa
    22141 taaaaatgat cggataccaa aggcgatgat tccagcacaa aaagaagcag gtgttatgga
    22201 agcctatttg ttttctgata ctaaagcaga aagtcagtgg ctaatcaaac aaattcagac
    22261 atttattcaa aaaaatggga aatataagga atcaatcata ttataccgat cagcttcaca
    22321 aacgcagcag cttgtccaat ctctgctgaa aacgaatatt ccttttgtac tggaagcaga
    22381 tagtccatat gaaggtatct ttggtatcaa gtctttccaa catttctatc aaaaaattgt
    22441 tcattggcaa acagcactga atctgcaaaa gaaaaatcag gcctaccagc aattgctccg
    22501 gcagctgatg gcagactgct acctgaaaaa aagtgaatgc gatcaatact tttctcctaa
    22561 atatcaaaat atagccgttg cagattatat tttgtcgata agccccaatt taaaatcaaa
    22621 atctgcagaa atccgagagt ttgaggaagc gttgcgtaaa acagaccagc atcaagaaat
    22681 ctattcgctt gttcaaagct atcttcatct gccacgtata gctaaggaaa tcgaccagag
    22741 tgatcaagaa tggatacgag aagaagtaaa gcagcatgct actttcagaa gactccaagc
    22801 cttatcgcaa gaaacccaag cgacagctaa agaattaaaa acccgcttga ttgcttacag
    22861 acaaggaaaa cttgatgcac tatgtatcca gagcattcac aaaagtaaag gtcttagtta
    22921 ccgaaatgtc tttttgattg gttgtaacga aggaagtttg ccttataacg gagcgacaga
    22981 aaagaaagta atcgacttta atgaaataaa agcagaacca gtgacgacga tagaagaaga
    23041 acgaagacta ttttatgttg cgatgactag agcaagaaat cgtctgtatc tatgtgtacc
    23101 tcaaatgaaa aattcaagaa aattgaaagt atcgagattt atcaaagaaa ccgggagtcg
    23161 tgtgaaaaaa ggaaagtgat agaagagagg gtgtgacaga agtggtcagt ttcaagaaag
    23221 aagaaggaat gtacgaaaat tgcttctcaa atttttgtga aatttcagct tattttcgaa
    23281 gaagctattt ctggaacacc gtttattcgg aaaaaggagg tgtgacaaga attttgtcac
    23341 acctccctta tttattttga aaatgagaat actattatca aaatagagaa tatatagaat
    23401 aattctcatt attcaaacgt aaataatcag tt
//
LOCUS       DACECY010000043        22787 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.044, whole genome shotgun sequence.
ACCESSION   DACECY010000043 DACECY010000000
VERSION     DACECY010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.044"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(41..1420)
                     /gene="brnQ"
                     /locus_tag="IWO38_001816"
     CDS             complement(41..1420)
                     /gene="brnQ"
                     /locus_tag="IWO38_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ0313904.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            2126..3097
                     /locus_tag="IWO38_001817"
     CDS             2126..3097
                     /locus_tag="IWO38_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ0313905.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            3121..5313
                     /locus_tag="IWO38_001818"
     CDS             3121..5313
                     /locus_tag="IWO38_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ0313906.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            5330..5806
                     /gene="ybeY"
                     /locus_tag="IWO38_001819"
     CDS             5330..5806
                     /gene="ybeY"
                     /locus_tag="IWO38_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ0313907.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            5784..6185
                     /locus_tag="IWO38_001820"
     CDS             5784..6185
                     /locus_tag="IWO38_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ0313908.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            6200..7099
                     /locus_tag="IWO38_001821"
     CDS             6200..7099
                     /locus_tag="IWO38_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ0313909.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            7242..8054
                     /gene="recO"
                     /locus_tag="IWO38_001822"
     CDS             7242..8054
                     /gene="recO"
                     /locus_tag="IWO38_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ0313910.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            8438..9355
                     /gene="glyQ"
                     /locus_tag="IWO38_001823"
     CDS             8438..9355
                     /gene="glyQ"
                     /locus_tag="IWO38_001823"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ0313911.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            9357..11432
                     /locus_tag="IWO38_001824"
     CDS             9357..11432
                     /locus_tag="IWO38_001824"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ0313912.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKITNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDIKTDRASRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLNQIAAMALSL
                     ASLDQIITK"
     gene            11822..12601
                     /locus_tag="IWO38_001825"
     CDS             11822..12601
                     /locus_tag="IWO38_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313913.1"
                     /translation="MEKPFVKSLTAVAVGAGAIVICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVIQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISVAYATKAQTVAQQMGYSNISY"
     gene            12647..13591
                     /locus_tag="IWO38_001826"
     CDS             12647..13591
                     /locus_tag="IWO38_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0313914.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGSDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            13607..14386
                     /locus_tag="IWO38_001827"
     CDS             13607..14386
                     /locus_tag="IWO38_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ0313915.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKRTAPARPKATNDKTAPSRRSRSRV"
     gene            14398..15096
                     /locus_tag="IWO38_001828"
     CDS             14398..15096
                     /locus_tag="IWO38_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ0313916.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            15124..15888
                     /locus_tag="IWO38_001829"
     CDS             15124..15888
                     /locus_tag="IWO38_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ0313917.1"
                     /translation="MIDLHCHILPGIDDGSENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            15902..17721
                     /locus_tag="IWO38_001830"
                     /pseudo
     CDS             15902..17721
                     /locus_tag="IWO38_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378780.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
     gene            17732..18364
                     /locus_tag="IWO38_001831"
     CDS             17732..18364
                     /locus_tag="IWO38_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ0313918.1"
                     /translation="MKAIIIGAGGFGKEIAFLLQSVSRYELIGFVDDSLKMKNQELLG
                     KPVLGTIDSLIELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVAPSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDAQIGNYNSIFPSVNISGHVILGD
                     KNEIGVGTKIIQNLRIGNMNIIGAGSVVIKNINNNTKNVGVPTKVIERWD"
     gene            18366..19484
                     /locus_tag="IWO38_001832"
     CDS             18366..19484
                     /locus_tag="IWO38_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ0313919.1"
                     /translation="MENKRILLASPHMSEEGYEQEYVKEAFDTNWVAPLGANVNGFEK
                     ELAKYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPV
                     FVDSDYETWNMKPEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKNELSKLSNIQF
                     MPENNWNEPNYWLSVITIKGINTNHLIDELDKNNIEARPVWKPMHLQPVFKNYDYIGD
                     DVAETLFDLGVCLPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            19477..20094
                     /locus_tag="IWO38_001833"
     CDS             19477..20094
                     /locus_tag="IWO38_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0313920.1"
                     /translation="MRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLGS
                     PIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKMLRATSLDELPE
                     LWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKFD
                     LDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNNTPEKFKGS"
     gene            20221..21390
                     /locus_tag="IWO38_001834"
     CDS             20221..21390
                     /locus_tag="IWO38_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0313921.1"
                     /translation="MCRVLNILFQYENIIKKYKEKRVMAKYYNKKILIMANNDDGLYR
                     FRGMLIKNLVDQGNEVIVSLPKGKNISKLIELGCQIIETPIDRRGINPKTDFKLFFNY
                     CNILKKIKPDMVIAYTIKPNIYGGLACRLRKVSYALNITGLGTAFQKKGVLKSLIVFL
                     YRLAAKKSKVIFFENFENMKTFQTLQIAKQSKYKLLNGAGVDVEHFSYLEYPKDEQVH
                     FLFIGRIMKEKGVDELFEAMKNLYSEEKNCVLDVLGYFEEDYGIKIEKYEKEGWLKYH
                     GYQSDIRPFVERAHCFVLPSWHEGMANTNLESAASGRPIITSDIPGCKEAVIESKSGY
                     LCERKNSCSLYNSMNKFLKLSYEQRINMGKMGRNYMIKKFNKKKVVDETIKSLFS"
     gene            21436..22260
                     /locus_tag="IWO38_001835"
     CDS             21436..22260
                     /locus_tag="IWO38_001835"
                     /inference="COORDINATES: protein motif:HMM:NF025025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0313922.1"
                     /translation="MSNVSNPLVSVIMGAYNSEKTIGRAIDSIIKQTYLHWEMIICDD
                     GSTDGTVKIIRKYVEKDKRIKLVQNKKNSGLAYSLNHCLKYSSGKYVARMDADDISLP
                     ERFAYQVGFFEKNPEYDVVGCNRIVFDENGDSGIRKSKEIPTINDMYKGSPFAHPTIM
                     MKRSVYTILGGYRVNKDTTRSEDLEMWFRFFNEGFRGYNLQRVLFKYQETSEDFHKRS
                     LRAAYGITKVLLRGYKLIGVSKYKYIYAFKPIISAIIPKYIMYRYQERRLHNTEVL"
BASE COUNT         7974 a         3812 c         4643 g         6358 t
ORIGIN      
        1 cctgtttaat atctcttatg ttaaattgtg tttgatttgg ttattcttct ggtagttcat
       61 ttgcttcttg tttataccct gataggcgat aggcgtaata aaagagtaag ctgccagcgt
      121 aaccaagtaa cgttgggacc atccagccaa gtcctaatgc agcaaaagga acgtgatgat
      181 gataaaatcc tagtaagctt gctacgacag tttgctgact cactaatgca ggcgaatttg
      241 ccaacatatc caatacggct ggaacggcag aaaagaacat cgttacttga tagacaaatg
      301 gactcttttg gaagaatttt gctgttaagg aaagcaagat taacgcaagt gacaatggat
      361 acaaaagcat caatactggt aaagaccatt ggatgatatt atccaatccg gcattcgcaa
      421 cgacaaaaga gacaaaagtc gttaaccgca gccatgtcat ataactgact ttagggaaca
      481 gtttatggaa atcttgtgca aaagaaacaa cgagtcccat agcggtagtg aagacaccta
      541 atgtgaccat tacccctaag aagctagcac cgaagttacc taaataaaaa ttagtgatct
      601 gagaaagtgc agttccgcca ttttctgata aaggcatctt attcaaactc atcgcaccga
      661 gaaggaccag accaaaataa atcatgactt ctaacgcaac gctcaggcta ccagcttttg
      721 ctgttaattc tgtgactttt ttatctttaa aaccaagatc acgaactgca tggacaaaag
      781 tgacacttaa agcaagtaaa gctactgcat caaccgtgtt gtatccttct aaaacgccat
      841 ttgtcaaagc gttcgtctga taagaatctg cagcagattg gtttagattc cccatgggtt
      901 ggaataaaga aacgacaaaa atgatagcta ataatgcaag aaacaaggag tttaaatact
      961 ttccgacata tttgaccaaa cgtgttggat gtacagtaag gaaataagca agtaaaaaga
     1021 aaagagcaga gaataaaaac atacctaatg aatcatattt ttcaggcaaa aagggtttag
     1081 cagccatttc aaaagcagtt gctgctgtcc gcggtgtacc aaagaatggt ccgatcgtta
     1141 aatgaacaag gatcaagaac aaggcagcat accattttcc gacaggttta gctaagtcat
     1201 acagaccatc actttttgtc actactacag ctaagatagc taataatggg aaaagagcgc
     1261 ctgagatggc aaatcctaca ccagcgctga tccagtttga tccagccatt tgacctaagt
     1321 ggacaggaaa gatcagatta cctgacccga agaacatccc gaagataagt gaactgacca
     1381 tcaataattg cttccatgaa taagtttttg tttcttccat aaatttttcc tccaataatt
     1441 atcattttga gtaacaaaat aaacgttgca gaaactagaa atgccactaa tatcagattc
     1501 catgaaaacc accaccttaa aatataaaaa aaagcccttt tccacttggg aaaagggctc
     1561 tagattctag aacggtacca ccttttttcg cttaaaaaag cttcttcatt cacggacgct
     1621 tttcgataaa gttatccgct agcctgataa tggaggcagc cacaatgatc tacgttttcg
     1681 acattgactc ggaaatgatg ttcgtctatt cgatttattg tcatttctca cttacatgac
     1741 tcactgggaa taaatcgctg aatagcctac tgttttcctc gacgctttgc tgtatattta
     1801 tttgttgacg ttataataca cctgtttttt ttgaaatgca agaatatttc aaatattttg
     1861 ttttcttttt ttgaaacaag aaaaataccc agagaatact tgctaagtta gacggaatac
     1921 ttgtcctcta tcttatgcat agcttacatg atcagctttt tttattcaaa cgagaccttt
     1981 agagaatgga agcagctatt ttttaacgtt tcttggtact tatggttaag caactctttt
     2041 agaacctttt cttatttgct atatagtttc ttctgagtat ggtaaaatag gaaagaatga
     2101 atcgaaaaaa aggggatgtc tatttttgac ggaagagtct agttctttag agattagatt
     2161 gacggctgct gatgatgtca gcatgctttt aggtgcacac gacaagcata taaaactgat
     2221 tgaagaaaca acagaaacaa ccatccatac acgtggagaa atcattcaga tcattggcga
     2281 acaatcccag atttccttgg ctgcctcagt cattcggaca ttgcaggaat tgatcaaacg
     2341 aggaatccat gtaagtacac cagatgtcgt gacggcactg aaaatggggc aaaaaggcac
     2401 attagattat tttgttgaaa tgtatgagga agagatcgtc aaagaccgta atggaaaacc
     2461 aatacgggta aaaaacagcg gccaaaagaa atatgtcgaa tcggtagcca aacatgatat
     2521 cgtatttggc gtaggaccag ccgggacagg aaaaactttt cttgcagtag tattagcaat
     2581 cgctgccttg aaaaaaggtg aagtgcaaaa aatcattttg acgcggcctg ctgtggaagc
     2641 aggggaaaat ctaggctttc ttcccgggga tctaaaagaa aaagtagatc cttatctgcg
     2701 tcctgtttat gatgctttat accaaatttt tggattagat catacgaacc gattgatgga
     2761 acgaggcgtg atcgaaattg ctcctttagc ttacatgcgg gggcggacat tggatgatgc
     2821 attcgttatt ttggatgaag cacagaacac gacagttgct cagatgaaaa tgttcctgac
     2881 acgtcttggt tatcaatcaa aaatgattgt caatggagat acgagtcaga ttgatctgcc
     2941 tcgagggaca acaagtggtc ttgttcatgc agaacgaaca ttaaagcaaa tcaagaaaat
     3001 cgattttgtt aattttgaag caagtgacgt tgttcggcac cctgtagttg ccgaaatcat
     3061 caaagcttat gaaaaggcag atctccatca agaataagct ttgcttagga ggataagcac
     3121 atgttgaata aaacactgca gaaactacaa gaaaaacttg gaaaggcagc attgcctttc
     3181 ctgctgttct tgttttttat cattatctgc ggcgttacat ttagtagtgt caaacaaaag
     3241 gccaatgatt tcagagaagg ccaagtagca gaagaaagta tccgtgcgaa caaaacgatc
     3301 gagaacacgg aagaaaccga acaaaaaaga aaactggcag cagaagcagt cactccagaa
     3361 tacacttacc aaaaagatct tgcagatgac cagaacaatc ggatcaagca actatttgaa
     3421 ttgatcgata aaacgaatga tgccatcaac aaatcttatg acgaaaagat tgataaggca
     3481 aaggaagatg aaaatatccc aaagcctact acagaagagc ggatcgccgc gttgaagaaa
     3541 aattttgaaa atgtgaatgc agaaaatgtt gcgttttatc aaaaactacc gaacaatttt
     3601 tattcgacga tatttgaaat gactgaaact gagatccata ctgtgaggga tgagagtctg
     3661 aagctcattg acgaacagat gagcaaacaa gttcgtgaaa gtgaattgga agcttttaaa
     3721 caagaagcag aagagcaaat ccaatatttg aatgtcacac cagcacaaca acaaatgatt
     3781 cgctacttag tggatcaagg tatcgtagtg aatgacgtat tgaatgagaa aaagacagaa
     3841 gaattgaaac aatccgcacg agaagctgta caaccagtga tgatcttcca aggagaaatc
     3901 attgttcgtg aaggtaatca aatcgatgct tcagctatga aaaaattgga attactagga
     3961 ctgactaatc aaacaacttc gatttttccg ctaattgcca tggttttagc tgttttgctt
     4021 caaatcggcg tactgattta taattcactt caattccaaa atgcaggaga aagaacaaaa
     4081 tacgtgcttt tctatgtgac agctatgtca gtcagcatag tgctgatgaa atttttccaa
     4141 ttattccaaa cggaacaagc tacttatatc ccgctgtttt atcccgctgc ttttgcacca
     4201 cttgttttaa gctttttctt gaaccgaaga gcagggatca tggctggtat tttccaagca
     4261 gtatctgcac ttttcatttt ttatgattcg attggcacga actttctaac gattatcttg
     4321 atgtcttatg ttttctctgg gctcatggca acgattgtca gacgcaaacg gatctcagaa
     4381 caaggattat ctgctgccat gtgggtcatt atttttcctg tgatgatgga tgtcattttg
     4441 atcgtctatc aaggaatgag ctttggtgat gcaacgactt ggttaatgct tgtctgcggg
     4501 ttcactggtg cactcttctc attcttgctg acgatcggtc tacacccata tattgaattg
     4561 atggtcaatg atgacagtgt aattgtatta aatgaactca gcaatccaaa tcatccttta
     4621 ttgaaacagt tgctggaaga agcgccaggt acttatcatc atagtatgat ggtagctaat
     4681 ctaagtgcta acgctgtagc agaaatcggc ggacgttctt tgctaactcg agtagcttgc
     4741 tattatcatg atatcggcaa gatcaaacat gctagctttt tcgttgagaa cttgccttca
     4801 ggagcggaaa acccgcacaa tttcttattg ccagaagaca gtaaacatat tatctttgga
     4861 catgtgacag atggagccaa aatattggaa gattataata tgccgcaaat ggtgatcgat
     4921 atttgcaaac agcatcatgg aaccacgcta atgcgttatt tctacatcaa agcgaaagaa
     4981 cgcaatccgg aagtaacaga agagcaattc cgttatcctg gaccaaaacc gcaaagtcgt
     5041 gaagcaggga tcgtcaatat tgctgacagt tgtgaggctg cagtacgggc aatggatcat
     5101 ccgacgagcg aaaaaatcga aagttttgtc cataatctta tcgaagagcg tatttccgat
     5161 ggtcaattag acgatagtgg actgaccttg aaagaaatcc gaaaagtgga aaaatcattg
     5221 atcagcggtt taagtagcac cttccattct cggatcaaat atcctaaaat gaaaacagaa
     5281 gttgagaaga taaaggaaga acaagaagaa tagaaagaag cgatgtataa tggatattac
     5341 ctttatggat gaaacaaacc aagtctcaga agagaaaatg aaagaaatcg atgacttgct
     5401 gcaatttgca gcatcttatt taaagctgcc agaggaaaca gaaatgtctg ttactttcat
     5461 ggacaatgcg gcaatccaag taatcaatcg tgattaccgt ggaaaagatg caccgacaga
     5521 cgtcatcagt tttgctctgg aagaagaagg cgaagacgaa atcccagtga tttttgaaga
     5581 tggagagaat ccattgcctc gtgtactagg agacatcatg atttctattg aacgtgctgc
     5641 tgaacaagca gaagcttacg gccattcata tgatcgggaa cttggctttt tagcggtaca
     5701 tggattcttg catatcaacg ggtatgacca tatgacacca gaagatgaga aagaaatgtt
     5761 tggtttacag aaagagatct tggatgccta tggactcaaa agataaaaac gtagagaaaa
     5821 acaaacattt cctgatgtct gtggaatttg ctgtccaagg agtcaaaacc gtttttgagg
     5881 aagaacgcaa tatgaggaaa catgttgcgt tcggtatcct tgctttattt atgggcgtga
     5941 ttttccagct tgatcggata gagtggttat ggctgctttt agctgttttt ctcgtttgga
     6001 tagtagagat catcaatact gtttttgaaa atgtagtgga catgtttacg gattttcatt
     6061 ttcatccgat tgggaaaaaa atcaaagata tggcagctgg agcagtttta gtgaccgctt
     6121 gttttgctgt catcattggg ttgattttat ttgtccctaa aatatatcat ttgttgttta
     6181 attaaaaagg agaacgtata tgccagaaca taaatcaggg tttgtcgcaa tcgtcggccg
     6241 cccgaatgta ggaaaatcca cattattgaa ccgaatcgtg ggtcaaaaaa ttgccattat
     6301 gagtgacaag gcacagacaa ctagaaataa aatccaagga gtatatacta caccagaagc
     6361 tcagctgatt tttatcgata caccaggaat ccataaacca aagcatcgtc taggagattt
     6421 tatggtagaa gcagcttaca gtgcgttgaa agaagtggat gcagtcttat ttatgatcag
     6481 tgctgatcaa aaacgcggcc ggggagacga ttttattatt gaacgtctaa aaaacgtgca
     6541 ttctccagtt tatctgatta tcaataaaat cgataagatc catccagacg agttgctggg
     6601 aatcatcgaa gattattcga cgcagatgcc ttttgcacaa gtgattccga tctctgctac
     6661 agaaggaaac aatgtcgaac gcttaatgga tgtattagtc agtgaaatgc cagagggccc
     6721 acaatatttt ccagatgatc aagtgactga tcatcctgaa tactttatcg tttctgaatt
     6781 gatccgtgag aaagtcttgt ttttaacacg agacgaagtg cctcattctg tcgcagtcgt
     6841 tgttgattcg atgaagcgaa acgagaatga taaaatccat atccaggcca cgatcatcgt
     6901 cgaaagagat agccaaaaag gaatcattat cggcaaaggc ggaaaaatgc tgaagcaagt
     6961 aggaacgaaa gcaagaaagg atatcgaaaa tcttttagga gacaaagtat ttctagaact
     7021 ttgggtcaaa gtccaaaaag actggcgaga caaaaaagtc tatctgcaag actttggcta
     7081 ccgaaaagac gaatactgat attctgaact tcttataggt taagtataga aataaactgg
     7141 ataaaaatcc gcacgttgag aaaaacggcg gaagatcgaa acataaataa tctggatcgg
     7201 cagaatctgc ttttccagat ttatttttta ggagggagaa tatgagccag cttgcagaat
     7261 cgaaaggctt gattttatct agccgtaatt acaaagaaaa agataaatta gtcaaaatct
     7321 ttacggaatc ttctggcaaa atgatgtttt atatcaaagg aatccatcgg caaaatcagc
     7381 cattagctcc ggctttgctc ccctttaccg aagcagtcta tatcggtaat tttcgaagtg
     7441 aaggtttgtc gtttttaagc agtgtcaaag acgtccatgc gtttcggaac atccaagaag
     7501 atatcttttt gagcggttat ggtacctatt tgctaaatct agtagatgca gcaatcgaag
     7561 atcatcaata tgacccgcat ttattccaat ttacgcaaca agctctgcag cggatggatc
     7621 aaggagatga tgcagaaatc atcacgaata tctttgaagt tcaaatactg caacgcttcg
     7681 gaatcgcgcc aatttggacg cactgtgtcg tatgcgggga aacaaaagga aaatttgact
     7741 attcttccaa atatggcggt gtgatatgcg agaaacactg gccaatggac gaacaccgtt
     7801 atcatgcaga tcctcgcgct gtttatttcg ttcgaatgtt ttcggctatt tcttatgata
     7861 agatcagtgg aatccacttg aaagaagaaa cgaagcaggc aatccgtgac ctcattgacc
     7921 agttgtatga tgaatatgtc ggtattcatc taaaaagcaa aaagttcatt gatcaaatga
     7981 agtcttgggc acacgtatta aagacgccag agaaaaatat gcctgaaaaa gagatacctg
     8041 aaaaagaaga ataaatgaaa gatacaacaa aaaaatcaaa actatttgac aaatacaggg
     8101 acttccgtct attattaacc gtagattaaa tagatcgttc actgagagga tctattatat
     8161 aaacaagaaa actgcaaaat gaatacgtac attgaccaag atcagtaaaa aacacgtggt
     8221 ttagcgagtt cgggagagtg gaagccggac ccaaacgatt tttgaagagg cacttgcgag
     8281 tacgataaat gaagctgccg aatctttatt cggaagtagg gtggaaccgc gaaattattc
     8341 gtccctatgt ctgtaatcag acatagggac tttttgtcgt tttctttcct gtattcttat
     8401 tgacagcaac gcttaaaaat tgaaaggggc aaaacagatg agtaaaaaat tgaccgtcca
     8461 agatatgatt ttaacattac aaaaattttg gtcatcaaac ggctgcatgc tgatgcaagc
     8521 ttatgataca gaaaaaggag caggtacgat gagcccatat acattcttac gggcaatcgg
     8581 accggaacca tggaatgctg cttatgtaga gccatcccgc cgaccagctg acgggcgtta
     8641 tggcgaaaac ccaaaccgtt tgtatcagca tcatcaattt caagtcgtaa tgaaaccttc
     8701 acctgaaaat atccaagagc tttatttaga aagcttgcgt cttttaggaa ttgatccatt
     8761 agaacacgat attcgttttg ttgaagacaa ctgggagaac ccatcgatgg gttgtgccgg
     8821 tgtgggctgg gaagtatggc ttgatgggat ggagatcaca caattcacgt acttccagca
     8881 agtcggagga ctaccttgta aaccagttac ttctgagatt acttatggac tagaacgtct
     8941 tgcttcatat attcaagaag ttgaaagtgt ctatgatctg gaatgggctg atggtgtgaa
     9001 atatggcgag atcttcaaac aaccagaata tgaacattcc aaatattctt ttgaagtaag
     9061 cgaccaagac ttgctactaa gcaattttga ccgttttgaa aaagaagcta aacgctgtat
     9121 cgatgaaaat cttgttcatc cagcgtatga ttatattttg aaatgcagcc atacattcaa
     9181 cttattagat gctcgcggtg cagtatccgt aacagagcgt gcaggttatc tttcccgtat
     9241 ccgcaacatg gctcgtgctg tagccaaaat ctttgtggca gaacgcgaaa aattaggatt
     9301 ccctttattg aataaaaaca cagcaaatcc agaaaacatg aaggaggctg aataagatgg
     9361 caaaagactt attacttgaa atcgggctgg aagaaatgcc tgctcatgtt gtcacaccaa
     9421 gcatgaaaca actagaagaa aaaacagcaa aatttttaga tgaaaaccaa ttgacttatg
     9481 atacgatcga aacgttttct acaccgcgcc gcttagcaat caaaataaca aacattccag
     9541 aacgtcaagc agacagtgaa gaagaagtca aaggaccagc caaaaaaatt gctttggacg
     9601 cagatggaaa ctggacgaaa gcagctgaag gatttgtccg cggacaaggt ctgacgacag
     9661 atgctatcac ttttagagaa ttaaaaggcg tggaatatgt ctatgtaacg aaatacactc
     9721 acggcaaaga agcaaaagaa gtattgacag gtttagcaga tgtcattaaa agcctcactt
     9781 tcccagtaac gatgcattgg gcagaatacg attttgaata tatccgccct attcactgga
     9841 tcgtggcatt gttagatgac gaagtaatcc cgtttgagat cttggatatc aaaacagaca
     9901 gagcttcaag aggtcatcgt ttcttaggtg aagatgtgac atttacgcat gcaaaagaat
     9961 acgaagaaaa attaaatgaa caatttgtta ttgcttcacc gcaaaaaaga aaagaaatga
    10021 tcatcacaca agcaaacgaa ttagcagaaa aaaatcactg gatcattgat ctagatgaag
    10081 atttactaga agaagtcaac aatctagtcg aatatccaac tgtcttttct ggcgacttcg
    10141 aagaaaaata tctatctgtt cctgaagaag tactagtcac ttctatgaaa gaacatcaac
    10201 gttatttcga agtacggaac gatcaaggta tgttgcttcc tcattttatt tctgctcgta
    10261 atggcgatcg ggtaaaattg gaaaatgtgg tacgaggcaa tcaaaaagta ttgactgctc
    10321 gtttagaaga tgcggaattc ttttataatg aagacaagaa agcttcaatc gaccaatacg
    10381 ttgaaaaatt aaaaactgtt actttccatg aaaaaatcgg tagcatctac gagaaaatgc
    10441 aacgggtaca agtcatcgct cgcatcatag gcgaacaagt aggactatct gaggaagaat
    10501 tgatggatct gcaacgcgct gctgaaatct ataaatttga tctagtaacc aacatggtag
    10561 gagaattccc tgaactacaa gggatcatgg gagaaaaata tgctctgctt aaaggcgaga
    10621 aaccagccgt agcaacagcg atccgtgaac attacatgcc tatatccagt gaaggagatc
    10681 tacctgagac tacagtaggt tctgttttag cgattgctga taaattagat agtatctttt
    10741 ctttctttgc agttgggatg atcccaagtg gctcaaacga tccatatgct ttgcgccgtc
    10801 aagcatatgg tgttgttcga atcatcgaat cgaaaaaatg ggcctttcct ttgtctgttc
    10861 tgcaagaaac gattagtgaa gttatcagta aagatacgga tcgttttggc attggcttgt
    10921 cagcgggaca acaacaggtg atcgacttta tcaaaggccg tctgcgccaa ttattgacaa
    10981 cgaagaatat tcgtcatgat gtcatcgaag ctgttttgaa cgcagaacaa aaagatctga
    11041 caaaagtatt tgcagcagca caattgttca agcagcatct tgcagatgaa gacttcaaac
    11101 catcgatgga agctttgaca cgtgtcgtga acttagcgaa aaaagcggag cttgaaaaac
    11161 aatcagaagt agaccctgaa ctgttcgaaa acgaagcgga gaaagaactg cacaaagcag
    11221 tagaggcaat gaaaacaagc tttgctgacc aaacgatcaa cgaaaattat gatcagcttg
    11281 taagccttcg cccactgatc gaaaattatt ttgatcagac gatggtcatg gcagaagaac
    11341 cagcagtacg taagaaccga ttaacgcaat taaatcaaat tgctgccatg gctttgtcac
    11401 tagcaagttt agatcaaatc atcacaaaat aatttctaat atgtaaataa accagagaac
    11461 aggcacaaaa taggctattt ctctggttta tttgttataa atataaataa ctattattaa
    11521 ttcaatgtta atttttttgt tattttattt tttgtgtaac ttttaaaaac aactatgaaa
    11581 attaaggaaa taaaaatgaa atcgattaat tgttatctta ttaatgtttt atttactttt
    11641 aaatcgtaaa ttgccctagt caagaaaaca gtttgtggta tgattatata gagaaaactt
    11701 gtttataaat atttctgtga atatattagt ttaattatgt tttttaaaat aaaaagctgt
    11761 gatttttatg taagtgaatt atatcttaat ttatctatca tcaatactta ggagtgaatc
    11821 tatggaaaag ccattcgtca agtcattgac tgctgtggcg gtaggtgcag gtgctattgt
    11881 gatctgtttg tttggttacc atatcaataa tcagcggcaa catcagcaac ggatcaatta
    11941 tgcagaatct gccatcacca atcaaaaaga caccttgact tctttatcga aagaagtaga
    12001 caagctgtat agtacaaaag aaaaaatttt tctaaaccct gaaataacag aagaaacaat
    12061 tacaagtttg tccaataagc tatcctctgt caaactttca gcagatgatt acgacatcaa
    12121 agaatcagaa ttgcctaagg aagctgttgc aatccaagat gagaagaaag caatcgtgac
    12181 acagttagat agtgcagaat caaaactgaa gatccagaca gctgtcaacg atttatttac
    12241 taaaaatgta tcaaactggc agcaagctgt agacgatgtc atcatcaaag aaaaggtgac
    12301 aagtgcagat gtcgctcgtg tacgtgaaaa tatgagcttt tttaaagata gtccttggaa
    12361 aacggttgtt atacaatatc taggattcgc tgatacccag atcgcgcaag tcacgcaact
    12421 agatcagtta ttcgatacga tgttaaagga tggtcaagtg actgccaccg ctacttacga
    12481 tcaatatttg acggcgctca gtcaaataga gcagatccgg aatgaaaaaa taagcgtagc
    12541 atacgcaaca aaagcacaaa ctgttgctca gcaaatgggc tatagcaata taagctattg
    12601 acagttaaag cgttgattca agcaaggtta gcaaacggag gaaactatgg caagacgaag
    12661 aagaaaatca agagggaaaa aaatatttct gattatttta gcagttctta tcgtattagt
    12721 attagcaatt gtaggagtag gagcaaaatt atatctggat ctctcacatt ctattgagaa
    12781 aacttacgaa tcagtggaac gaacccaagg agaacgaacg aaaccagttg atctggacaa
    12841 acaagaatct ttctctgttc tattgatggg gattgatact ggagatttag gtcgggtcga
    12901 tcaaggaaga tcagatacga tgatggttgc cacagtcagt cctgaagaca aacagacaac
    12961 gatcgtcagc attcctcgag atacgtatgt cgacatcgta ggtagtgaca caaaagacaa
    13021 gatcaaccac gcttacgcat tcggcggagc tgccatggcg atggataccg tacaaaaata
    13081 tgtagacatc ccaatcgatc actatgtctc tatcaatatg aaagggctaa aagaacttgt
    13141 tgatgcagtt ggtggtatcg aagtcaataa tgacctcact ttctcacaag atggctatga
    13201 ctttacaatc ggtaaaatta ctttgaacgg ggatcaggca ttatcttatt ctcgtatgcg
    13261 ttatgaagat ccaaacggtg actatggtag acaagagcgt caaagaaaga tcgtagaagg
    13321 tgtagcgaaa aaagtattga gtctaaacgg tgtcagcaac tatcaatccg ttttgaatgc
    13381 tctagagtcg aatatgaaaa cagacatgag ctttgatgaa atgaaaaaaa ttgctttaga
    13441 ttatcgggag gcatttggta cgatcaagca agatcagctg caaggagaag gcttcatgca
    13501 agataatatc tcctatcaaa aagtcagtga tcaagaactt gatcgtgtga aaaaagaatt
    13561 aaaagatcaa atgaatctag agaataaata ggaaagtgga gaaaaaatgg aagagacaat
    13621 cagcttgaaa gaaatttttg aaacattacg caaacacttg gtaacaatca tcatctcaat
    13681 gtttgctggc ttggccatct caggaatcgt aacatttttc atcttgacac caaaatacag
    13741 ttcacaagca caattgatcg tgacactgcc acaatcagac acagcaaatg tcaatgatgt
    13801 caatacgaac ctgcaaatga tcaatacgta taaagatatg atcgtcagtg atttagtact
    13861 aaatgaagtc aaagatcgtt tagaaacaga agacaatgtg aaaatgactg ctggacaaat
    13921 caaagatgcg atctccgtca atcagtcaca aaattcacaa atgttctcga tccaggcaat
    13981 aagcacgaat gcagtgactg ctcaacagat cgctaataca acttcacaag ttttccaaga
    14041 aaacgcaaaa gacgtgatga atgtcgataa aatctcgatt atttcaggtg cagttgccaa
    14101 tacgacacct gtttcgccaa ataataaatt gaatctagtg attggcttag tattaggaat
    14161 gatggtaggc gtaggtttgg cattcttatt cgaattactt gatcggacag tgaaagatga
    14221 aaaatatgtg acggatacat taggattccc aatcttagga acagttccag aaatgtcagc
    14281 gaaagaattg aacgctggta tccgtaaaaa aagaacagct cctgcacgac caaaagctac
    14341 aaacgataaa acagccccat cacgcagaag ccgctcaaga gtctaggagg aagaaaaatg
    14401 gcacgaacac agaaacaaaa agcaacacaa actcatgcag tcagtttgat cacattaaca
    14461 aacccatctt caccaatcgc cgaacaatat cgtacgatcc ggacgaatat ccagtttgcc
    14521 tccgcagctg gtcagcaaat caaaacgatt gtcgtgactt catcaggtcc tggagaaggg
    14581 aaatccacca ctgcagcaaa cattgcagtc gtttttgcta aatccggtca acgagttcta
    14641 ctagtcgatg cagatttacg taaacccgtc gtctataaga cattccagtt gaacaatgcc
    14701 agtggattaa gtacagcact tagttcatca ggaagtgtag cagatgtgat ccaacgaacg
    14761 ccagtcgaga acttgtcgat tttgcctagt ggacctaagc cacccaaccc atcagaattg
    14821 ttaagctcgc caagaatgga tcagatccta gctgaagcaa gacagttatt tgacgtggtg
    14881 atattcgata tgccaccagt agtagccgta acagatgcac agatcatgtc ttcaaaaaca
    14941 gatggcactt tattggtcgt acgtgaaaat atgtcacgta aagaatcctt gactaaagca
    15001 aaagaactgc tagagatggt acaagcacga gtcttaggtg tcgtatacaa cggagcagaa
    15061 cacagcaaag atgctggtta ttactacgga aattaaggaa taagaagaga ggagacaaga
    15121 gaaatgatcg atctacattg ccatatccta ccaggaatcg atgacgggtc agaaaactta
    15181 gaagcaagta ttgcaatggc tgaaaaagcg attcagcaag gcattacgca tattttatgc
    15241 acaccacacc acaataatgg aaaatacagc aatgaaaaat cacaagtcat ttctctagtc
    15301 gcttcgttac aagccgaatt agagaaaaga cagttgcctt tgacgttatt ggaaggacaa
    15361 gaagtacgta ttaccggaac gctcatcgaa gacatccacc gagatgaaat tttatttaca
    15421 gatttagatg atacatatct tctgatcgaa tttccgacac tggaagtgcc tttatatgca
    15481 gaacggcttt tttttgagtt gttagaacta ggaaagacac cagtgatcgt ccatccagaa
    15541 cgcaacgcgc attttcgaga agacccaaat catttgcttc cgttcctaga tatgggatgt
    15601 ttggcccaac ttactgcgcc aagttatgtt ggtgtatttg gcaaagacat ccagaaaaca
    15661 gccaaaacaa tggtcaaaca taatctcgtg cagatggctg cttctgatgc acacggggtc
    15721 tctaagcgca ccttttactt aaaagagtgc tatgagcaga ttgccaagga gtttggtaag
    15781 gaaaaagtag tacagatgaa acaagtcgca aaggatctga tcaacgggga tgagatcaat
    15841 tatccaatct atgaagaggt aaaaaagaag aagtttggtt tattttaggg ggagttaaaa
    15901 agtgtttata ttaacaagaa gaagaaaaat tgccacattg gttgttgtgg acagcttgtt
    15961 attaatcgct gcaaatattg cagcagctaa attcatgaag ccgtttgtat ctataccaat
    16021 ggatcttatt ttaatctcta tcggtttgtc gattggtttt tatttatttt atggtagttt
    16081 attcaaagtt ttcactcgga tcaatcgcta tacaaattta agagagatca ttgcaatttt
    16141 tagttcacta tctgcaagtg ctgcctcaag tatcttcata ctgctgttta ttaatcggcg
    16201 atacagttta cggttagtta tctttactta tctgctttcg ctcttattga tcatcggtag
    16261 ccgattgatc tggcgtatct acatcgaaaa aaaaaaaata tgagatatgt atcagcagat
    16321 tctgctaaaa atacattgat tgttggtgct ggcgaaggcg ggcgtatttt atacaacagc
    16381 tttcttggat ccaaaacggc tcaagatatc cacgttgtcg ggtttgtaga tgatgatcca
    16441 aataaaagaa atacgtatct ttcaggaaaa aaagtattag gtgcattaaa agatattccg
    16501 gaattgattg aaaaatatga cattcaaatg gtaacgatcg ctatcccttc cttatcaaga
    16561 aaaaaattac gacgaatctt cgagttagtc gaatccgctc gtgtgaaagt caacacgatg
    16621 ccttctatag aagaacttgc ttcagggaaa attagcgtat caaaattaaa aacaattgac
    16681 gtagtggatt tactgggaag agatgaggta aagctggata ttgaatccat caaagatcag
    16741 atcacagaca aagtcattct tgtcaccggc gcaggaggat cgatcggttc agaaatctgt
    16801 cggcaaatca ttcaattcaa tcctgcaaag ctattgcttt taggacatgg ggagaactcg
    16861 atctacttga ttgaccgtga gttacgcacg catcatcaaa attgtcctac agaaatcgta
    16921 ccgatcatcg cagatatcca agatcgcgag aaaatcaatg agatcatgga acaatatcat
    16981 ccagatatcg tctaccatgc agctgctcat aaacatgtac cactgatgga atataatcca
    17041 aaagaagcag tcaaaaacaa tattttcggt acaaaaaatg tagcagaagc agctaaagca
    17101 gcaaaagtga aaaacttcgt catggtatcg acagataaag ctaacaatcc tccaaatgtc
    17161 atgggttcaa caaaacgaat cgctgaaatg attgtgactg gattgaatga agaaggctgt
    17221 acgaaattct cagctgtcag atttgggaat gtccttggtt ctcgcggtag tgtgattcca
    17281 gtattcagag aacaaattgc tcaaggtggt ccaatcactg taacagactt tcgtatgact
    17341 cgatacttta tgacgattcc tgaagctagt cgtttagtta ttcaatctgg cgcattagca
    17401 aaaggtggcg aaatttttat tctagatatg agtgaaccag tgaagattgt agacctagcg
    17461 aaaaacatga tccgattgag cgggtatagc gaagacgaaa tcgaaatcat tgaaacaggt
    17521 atccgtccag gggaaaaact atatgaagaa ctattgttgg ataaggaaag aaatgatgaa
    17581 gcagtttatg agaaaatctt tgtaggaaac atcaaaggct actcaataca aaaagtgatg
    17641 gattttgtta agagcctgcc gcaagacgat gaacaattgg ctaaagacat tgtcactttt
    17701 gcaaatgctt cgaataagta gggggagtaa agtgaaagca attattatag gagctggtgg
    17761 atttggcaaa gagattgctt ttctattaca aagtgtctct agatatgaat tgattggttt
    17821 tgtcgatgat tcgttaaaaa tgaaaaatca agaattattg ggaaaacctg tattaggaac
    17881 aatagattca ttaatagaat tagaagaaga gactgctatt tttctgggaa ttgcttcgcc
    17941 agatatcaag gagaaaatat atcaaaagat aaataaaaat aataaactga ttttccctaa
    18001 tttagttgca ccaagtgcac tagtgggcat aaatgttcag ttagggattg gcaatatttt
    18061 gatgccctat acgacttata ctgctgacgt agttttagga aatttcaata tgatcaatat
    18121 cggctctact atcggacatg atgctcaaat cggtaattat aattcaattt ttccaagtgt
    18181 aaatatttca ggacatgtta tattaggaga taaaaatgaa attggagtag gtactaaaat
    18241 aatacaaaat ctaagaattg gaaatatgaa tattataggt gcaggaagtg tagtaataaa
    18301 aaatattaat aataatacta aaaatgttgg cgtgcctacc aaagtaatag aaagatggga
    18361 ttaagttgga aaataaacga atattattag catctcctca tatgagtgag gaaggatatg
    18421 aacaagagta tgtaaaagaa gcatttgata caaattgggt tgctccttta ggtgcgaacg
    18481 tgaatggttt tgagaaagaa ttagcaaaat acgtgggagc tagtcatgct gccgcattgt
    18541 cttctggaac agcagctata catttagctt tgaaagcggc tggggtcggt aaagatgaca
    18601 tagtgatttg tcagtccttg acgttctcag ctactgctaa tccaattctc tatcaaggag
    18661 ctatacctgt atttgttgat agtgattacg aaacttggaa tatgaaacct gaacatttgc
    18721 gaacagcact agaaaaatat cccaacacaa aggctgtatt agtagtacat ttgtacggtt
    18781 tatcagctaa tttagacgaa attactaaaa tatgcaaaga atatgatgtt actctaattg
    18841 aagatgctgc agaatcatta ggaacaacgt ataaaggaaa atatactggt acatttggcg
    18901 actatggaat ttattctttt aatggaaata agataatcac aacttcaggt ggcggaatgc
    18961 ttgtatctaa tgacaaagaa aaggtggaaa aagtgagatt ttgggctaca caatctcgtg
    19021 agaaggcacg ccattatcaa cacaatgaaa ttggctataa ttatcgaatg agcaatatcg
    19081 ttgcaggtat cggtcgagga caattgaaag ttttaaatga aagaattgag aaaaaacgat
    19141 atattcatag tttttataaa aacgaattgt ctaaattatc aaatatacaa ttcatgccag
    19201 aaaataattg gaatgaacca aattattggt taagtgtcat aacaataaag ggaataaata
    19261 cgaatcatct tattgatgag ttagataaaa ataatataga agcccgccca gtatggaaac
    19321 caatgcattt acaacctgtt ttcaaaaatt atgattatat tggcgatgat gtagcagaaa
    19381 ctttatttga cttaggagta tgtttaccat cagatacaaa gatgacagat gaagatttaa
    19441 aatatgtagt atctacgatt aaaagagtga tcaatgatgc gtaaaaaaac tttctatcag
    19501 ctttttggga aaagaatttt agatatccta ttgtcaggaa ttgctctcat cgttttatcg
    19561 ccgataatct taattgtagg ttttttagta agaattaaac ttggttctcc aattatcttc
    19621 aaacaagaac gaccaggaaa aagtgaaaaa attttttcca tgtataaatt tcgtacaatg
    19681 actgacgaaa gagaccacaa tggagaatat ttacctgatg aaattcgact aactaaattt
    19741 gggaaaatgc ttagagcaac aagtctggat gagctacccg aactttggaa tattttgaaa
    19801 ggtgatatgt ctatcgtagg acctcgtcca ttattagtgg agtatttgcc actatatagt
    19861 gagaagcaac ggaaaagaca caacgtaaga ccaggattaa ctggactggc tcaggtcaat
    19921 ggaagaaatg ctattagttg ggaagaaaag tttgatttag atgtttatta tgttgataaa
    19981 attagtttct ttaatgatct tattattata attcagacat gtaaaaaagt aatcaagaaa
    20041 gagaatatta atacaatcaa taacaatacc ccagaaaaat ttaaagggag ttaaatacag
    20101 cagcttaatg caagttttgt gacttgcatt atatttaggt ataaatcaaa gcgagtatat
    20161 tagaattata taacatatgt taaaaaaacg catagctaat aaaagtatga ttttaaaaat
    20221 ttgtgtagag ttttaaatat actgtttcaa tacgagaaca ttataaaaaa gtataaggaa
    20281 aagagagtta tggcaaagta ttataataaa aaaattctta taatggcaaa caacgatgat
    20341 ggtctttacc gttttcgagg aatgctcata aaaaacttag tagatcaagg taatgaagtt
    20401 attgtatctc ttccaaaggg caagaatatt tctaaattaa ttgaacttgg atgtcaaatt
    20461 attgaaactc caatagatag aagaggaatt aatccaaaaa ctgactttaa actatttttt
    20521 aactattgta atattttgaa aaaaataaag ccagatatgg tcattgctta tactatcaaa
    20581 ccaaatatat acggtggatt ggcatgtcgg ttaagaaagg tttcttatgc attaaatata
    20641 accggattag gaacggcatt tcaaaaaaag ggggttttga aaagtcttat tgtatttttg
    20701 tatagattag cagctaaaaa atctaaggtc atatttttcg aaaattttga aaatatgaaa
    20761 acatttcaga ctttacaaat tgctaaacaa agcaaataca agctattaaa cggtgcagga
    20821 gtagatgtag aacactttag ttatttagaa tatcctaaag atgagcaagt tcatttttta
    20881 tttattggaa gaattatgaa agagaaaggt gtagatgaac tttttgaagc aatgaaaaac
    20941 ttatatagtg aagagaaaaa ttgtgtactt gatgtccttg ggtattttga agaagattac
    21001 ggcataaaaa ttgaaaaata cgaaaaagag ggatggttaa aatatcatgg atatcaatct
    21061 gatattcgtc cctttgtaga aagagcacat tgctttgttt tgcctagttg gcacgaagga
    21121 atggcaaata ctaaccttga atcagcagca tcagggagac caattattac aagtgatatc
    21181 ccagggtgta aagaagcagt tattgaatcg aagtctggtt atttatgtga aagaaaaaat
    21241 agttgtagtc tctataattc gatgaataaa tttctaaaat tatcatatga gcagcgcatt
    21301 aacatgggaa aaatggggag aaattatatg attaaaaaat ttaataagaa gaaagttgtc
    21361 gatgaaacaa ttaaatcatt atttagttaa aaagatatgt tttgcaaagt atgtgataga
    21421 aaagagggat tctgtttgag taacgtaagt aatccattag taagtgtaat catgggagcc
    21481 tacaattcag agaaaacaat tggaagagca attgattcaa ttataaaaca aacatattta
    21541 cattgggaaa tgataatttg cgatgatgga tcaactgatg gaactgtaaa aataatacgg
    21601 aaatatgtag aaaaggataa acgtattaag cttgttcaaa ataaaaaaaa tagtggatta
    21661 gcatattcat taaatcattg tttgaaatat tcatcgggaa aatatgttgc aaggatggat
    21721 gcagatgata ttagtttacc agaaagattt gcataccaag ttggattctt tgaaaaaaat
    21781 ccagaatatg acgttgtagg atgtaatcga atagtattcg atgaaaatgg tgattctgga
    21841 atacgaaaat caaaggaaat tccaactatt aatgacatgt ataaaggttc tccatttgct
    21901 catccgacaa taatgatgaa acgaagtgtt tatactattc taggtggtta cagggttaac
    21961 aaagatacaa caagatcaga agatttagaa atgtggtttc gattcttcaa tgaaggattt
    22021 agaggttata atttacaacg agtcttattt aaatatcaag aaacaagcga agattttcat
    22081 aaaagaagtt taagagcagc ttacggaata acaaaagtat tgttgagagg ttataagtta
    22141 ataggagtta gtaaatataa atatatttac gcatttaagc cgattatttc cgcaataatt
    22201 cctaagtata ttatgtatag atatcaagag aggagattac ataatactga agtattatga
    22261 tattgataaa attatgattt taacaattat tatttttgct gatatgctaa cttttttgtt
    22321 tatacgtaga tgtatatttg attactacca taaaatattt gcgtatttaa tgttaacagg
    22381 attcactttg tattcaggta tcggtattca atttcagcaa aataaaatat ctactccata
    22441 catatatcta atgcaatttg tgttatttat gattgtattt gtatttatga tttggtttag
    22501 tgaacggtta aaaaaaggaa aagcattaag tgattcagat ataatattaa atagtagcat
    22561 tttgattgta tttactataa tatacttatt cactttttta tatcaattga tttatagcgg
    22621 tcgttctata aatagtttta cagatattgt taacatattc attcattata gtccaacaac
    22681 ctttagtgag agattgtcta gaaaaaataa tgagatgtac gtgattatga cgaatcagat
    22741 acgttccatt gcagcaccat ttttttatat ttatttatat aaagtaa
//
LOCUS       DACECY010000044        22777 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.045, whole genome shotgun sequence.
ACCESSION   DACECY010000044 DACECY010000000
VERSION     DACECY010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.045"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..293)
                     /locus_tag="IWO38_001836"
     CDS             complement(<1..293)
                     /locus_tag="IWO38_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313923.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPER"
     gene            complement(542..742)
                     /locus_tag="IWO38_001837"
     CDS             complement(542..742)
                     /locus_tag="IWO38_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0313924.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(1047..2279)
                     /locus_tag="IWO38_001838"
     CDS             complement(1047..2279)
                     /locus_tag="IWO38_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ0313925.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2533..3102
                     /locus_tag="IWO38_001839"
     CDS             2533..3102
                     /locus_tag="IWO38_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0313926.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3280..3720)
                     /locus_tag="IWO38_001840"
     CDS             complement(3280..3720)
                     /locus_tag="IWO38_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ0313927.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3878..4642
                     /gene="map"
                     /locus_tag="IWO38_001841"
     CDS             3878..4642
                     /gene="map"
                     /locus_tag="IWO38_001841"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ0313928.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4674..5597
                     /locus_tag="IWO38_001842"
     CDS             4674..5597
                     /locus_tag="IWO38_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ0313929.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5673..6812
                     /locus_tag="IWO38_001843"
     CDS             5673..6812
                     /locus_tag="IWO38_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ0313930.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6805..7605
                     /locus_tag="IWO38_001844"
     CDS             6805..7605
                     /locus_tag="IWO38_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0313931.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7605..8432
                     /locus_tag="IWO38_001845"
     CDS             7605..8432
                     /locus_tag="IWO38_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0313932.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8410..9144
                     /locus_tag="IWO38_001846"
     CDS             8410..9144
                     /locus_tag="IWO38_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0313933.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            9244..10110
                     /gene="rfbA"
                     /locus_tag="IWO38_001847"
     CDS             9244..10110
                     /gene="rfbA"
                     /locus_tag="IWO38_001847"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ0313934.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            10124..10696
                     /gene="rfbC"
                     /locus_tag="IWO38_001848"
     CDS             10124..10696
                     /gene="rfbC"
                     /locus_tag="IWO38_001848"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ0313935.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10718..11746
                     /gene="rfbB"
                     /locus_tag="IWO38_001849"
     CDS             10718..11746
                     /gene="rfbB"
                     /locus_tag="IWO38_001849"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ0313936.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11844..12695
                     /gene="rfbD"
                     /locus_tag="IWO38_001850"
     CDS             11844..12695
                     /gene="rfbD"
                     /locus_tag="IWO38_001850"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ0313937.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12730..14763
                     /locus_tag="IWO38_001851"
     CDS             12730..14763
                     /locus_tag="IWO38_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313938.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14807..16087
                     /locus_tag="IWO38_001852"
     CDS             14807..16087
                     /locus_tag="IWO38_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ0313939.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            16297..17103
                     /locus_tag="IWO38_001853"
     CDS             16297..17103
                     /locus_tag="IWO38_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0313940.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            17115..18335
                     /locus_tag="IWO38_001854"
     CDS             17115..18335
                     /locus_tag="IWO38_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0313941.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18325..19911
                     /locus_tag="IWO38_001855"
     CDS             18325..19911
                     /locus_tag="IWO38_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0313942.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19950..22088
                     /locus_tag="IWO38_001856"
     CDS             19950..22088
                     /locus_tag="IWO38_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0313943.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALKMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(22156..>22777)
                     /locus_tag="IWO38_001857"
     CDS             complement(22156..>22777)
                     /locus_tag="IWO38_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAQ0313944.1"
                     /translation="KGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFN
                     SEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKN
                     FFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMD
                     FRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         7177 a         3858 c         4706 g         7036 t
ORIGIN      
        1 tacctttctg gatcaacgaa agtatacttt atatagtcat aacgccgatg atcgctacgt
       61 gcgtccggca cgtcagtcac gatatcaaac aaaaagtata catctttctt cattctagtt
      121 tttgcagcag ggattttaaa gtagttctta ttagaatagt agagattgat taataagcta
      181 tcttcgattg ctaaaaagaa aacttctgaa ttccatactt tataaaaatc tttgataaat
      241 ctattcgaag ggtcaaattt aaaccataat tgtgtttttc cttccatcag cataaaagtt
      301 cacctcaaaa agagtatacg aacgaatgtt ctttttgtga aggcggaatt taatatactg
      361 aataatagaa aacaagtact tgtgccaaat tatgtgccaa aaaaattcga attcaacaaa
      421 atgtaaacaa atagaaaacc ttctatatta agatttcctg caataacaaa cacaacagaa
      481 gacgtgtaat agttagtcag gaacgtacaa ataacccctg tatcctttgc ggtacagggg
      541 gttattttgt ataagtagac attgtagttt ctaaactgct atgtcctaaa cgtttcgata
      601 cgctaagtat atttactcct tgataaagta aaatagatgc atgcgtatgt cttagtccat
      661 caataaacag tcgaagagaa aatagggaaa aattgattac taaagaaaaa cttactaggt
      721 cgacggtgta gttgattttc atacttcaat agattcgaat taaacgattt cgattggctt
      781 ataccagtcc tatccaacga aagatatatc gttcttaact ctagcggtat aacttgaagc
      841 tttacgtaaa gccaatggga atgaactgga gatgactttt tattattttt agtaatagct
      901 gatgggttaa tacttacaac tttccttttc aaagtaccat ataaaaaaac ggtggggttg
      961 tttagcgaaa taaaggatct aagccaatag cttagatcct ttattctatg ctttatatta
     1021 ttttttgcat gtgtgcgaaa gtatactcaa gcccaatcgc catttcggaa aatcggtact
     1081 gtagagccat cttcacggat tccgtcgata tccatcttgt cagatcctac catgaaatct
     1141 acgtgggttt gacttctatt caatccagct tctgctaatt cttcatcgga catttctgta
     1201 ccgcctttca cactgaaggc ataagcagaa cctagtgcta agtgattcga tgcgttttca
     1261 tcaaataatg tattgaaaaa gatgatgcca gattgagaaa taggtgaagg gtcaggtaca
     1321 agtgcgactt ctcctaaacg acgcgcacct tcatctgtat caagcagttt tgctaagaca
     1381 tcttctcctt gttcggcaga aaaatcaaca acttttccat ctttaaaagt aaatttcata
     1441 cccgagatga tggtgccagc ataactcagt ggctttgtac ttgaaatgta accatctact
     1501 cgatgactgt caggtgctgt gaacacttct tccgtcggca tatttgccat gaatttttct
     1561 ccacgagcat tatagcttcc agcgccttcc caaagatggt ttttaggcaa tccgataatg
     1621 atatctgtac cgggagcagt atagtgaagg gcagaaaatt gttctcgatt caattcttct
     1681 gctttttttg ctaatttttc atcatgcttc ttccatgcta agacagggtc ttcttcatag
     1741 acacgagtcg ttttgaaaat ctgatcccat aatgcctcaa cttgtttttc ttctggcagt
     1801 tctgggaaga cttttgctgc ccattgttta ccagccgcag ccacgacagt ccaactgact
     1861 ttatttgctt gagttgcctt gcgtaaattc attagtgctt tgccattcgc tgattgataa
     1921 gacgctactc gatggctatc tacaccagca aatgcatcgg gatcggcaga aacaacgctg
     1981 atcctgcttg ctcctttttc aagccattca tccgcttgat cgattttaga ttgtggtaca
     2041 ttctcaatac gatccgttgc agcatgtaaa agaaattctc tttggatctg gtcgtcagtc
     2101 cattgaacga taacctcagc ggctcctagt ttataagctt cttgtgtgat caatcgagca
     2161 aggggtgcct gttccacgct gatctgtaaa acaactgtgt gtcctttttc tgtggctacg
     2221 ccagtttctg caattaagcg tgcatatttt tttagaagtt cattaaaatc agataacata
     2281 ataaaaattc ctcctcacca ttttttctgg taactattag catgatacca tttaattatg
     2341 attcaaacaa tgaggcttta aataaaaaaa ggaggttatt tttttaaggt taagtttcat
     2401 gatatatgtt actacttgct atttcagctt gtgaagaaaa tcaaaaacta attttgcata
     2461 tcaaaaaaac atgttacaat gaaattaatc aatggtggaa gcgttataaa aaaattaagg
     2521 agattaaaaa atatggcaag aaaaaagacg attactagag aacagatttt ggcagctgct
     2581 tatgaagtag ctgccaccga tggatttaca cgatttactg cgagaaacat cgcgaacaaa
     2641 atgaattgtt ccacgcaacc gatttatctt gagtttaaaa atatggatga cttgagacaa
     2701 gctctttttg agcagatcta tgatcattta caatatgaag tgttcactgt tgagcataca
     2761 gggaatacga ttatcgatct tgcttttaat tatatccatt ttgcaagaag agagaagaag
     2821 ttgtatcgtt cactgtactt agaagaatat ggtggaggaa aagagatgca gaatttctct
     2881 tatacttact ttgtgaatac agtgaaacgc gatccagctt atgtgaatct ttcagaagag
     2941 cagatcgaat ctttgtataa tgggacatgg atcatagcta ctgggatcgc agctttgatg
     3001 tcttcaagca tcatccatcc aactgatgaa cagattgaaa agatgatcca agacagtata
     3061 gatgctatct tgaaattcaa agaaccaatc gaaatcgatt gaatatactc aaaagttcca
     3121 caaatcagta ggtaacaaga gatgtgtcaa aataaggaaa agttcgtttt tcttacagcg
     3181 tatgcatttt taactcccga agaactcttc catgaatgca atgaataaaa gagccataca
     3241 gattgaataa atctggatgg ctcttttatt gcaagtatat taaatttttg cgactagttc
     3301 tttagcgaaa gcttctaaat tttcgatatc ttcttcttca gcattcagat ccactttaac
     3361 attttcggct ccttttgatg cgcctgtttt tgcaaaagca gcatcgaaat catccaccga
     3421 tttgcagaat tcatcgtaaa aagtatcacc agatccgcag acaccgtaaa tttttcccaa
     3481 tagatccaat tcttgcaagt cttcataaaa gtctacgatc tcatcaggca gatcgccatc
     3541 atcgtatgta taagtagcta ctacgcaaat atctgcctcc tcgaattcat ctgcatctac
     3601 ttgggtacat tcgttgattt cgacttcgat atccagattt tcaaatgctt ctgcaacaat
     3661 atcggcgatt tcttcagtgt ttcctgtcat gctagcatat acgatttttg ctaaagccat
     3721 gatgtgcctc ctcgtttata attatttaac aattatgaat tataccaaaa catcattgaa
     3781 aagaaaataa aacctaaaga cacctgggga atgtcatttg gctaaatgta tgccgctatg
     3841 ttaaaatgag agggatttgt aaaaaggaga cgaaaatatg attactttaa aatcagcacg
     3901 tgaaattgaa atgatggcag aatctggagc tttattagca gatgtacata aaaatctgcg
     3961 tgattttata aaaccaggaa tcacaagttg ggatattgag gtatttgtca gaaactatat
     4021 cgagagtcat ggcggaattg ctgcgcaaat tggctttgaa gggtatgaat atgcgacttg
     4081 tatcagtata aatgatgaaa tttgtcatgg attccctcgt aaaaagcctt tgaaaaacgg
     4141 tgatttggta aaagtcgata tgtgtatcga tttgaaaggc ggcatttctg attcttgttg
     4201 ggcctatgta gttggagaat ctacacctga gatcgaccga ttgatggaag taacgaagaa
     4261 agctttgtac atcggtattg agcaagcaca agtagggaat cggattggtg acatcggtca
     4321 tgctatccaa acttatgtag aaggcgaaaa tctagcgatc gttcgtgatt ttattggtca
     4381 tggcgttggt cctacgattc atgaagaacc agcagttcct cattacggag aagctggaaa
     4441 agggttgcgg ttgaaagaag gcatggttat cacgatcgaa cctatggtta ataccggaac
     4501 ttggaaaatg aaaatggatc ctaacggctg gactgcgtat acaagagacg gcggactttc
     4561 ttgccaatat gaacatacat tggctattac taaagatggt ccaagaattt tgacatcaca
     4621 aggagaagaa ggcacttatt gatctatcca gtcaataggt acgggagagg tacatgaaag
     4681 tttggaacaa aataaaacaa aatgaaaatc tgatgcgctt tattgagacg actcaaaagc
     4741 gcatcactga ttcagaaatg agtacgacct ctgtcgtcgt tgcttattat ttactcttgt
     4801 ctttatttcc attgattatt gcttttggta atattttacc gtttctccac atcgatcaag
     4861 agactgtact gacctattta cgagaagtga ttcctgagac cgtttaccag tttatcggtc
     4921 ctgcaatcaa agaccttttg acacaaagtt caggaggctt gttatccgtt tctgcattag
     4981 cggctttatg gtctgcaagt caaagtatca atgctttgca aattgcgatg aataaagcgt
     5041 atggcgtaga aaatagaaaa aattttttga tcgtccgttt cttctcacta attgtcattg
     5101 tcttgttcat gattgctatt agtggggtaa ctatcgttct tgggttggga cagctgattt
     5161 tggatgctat tcagccaatc gtacaaatac ccgtgaactt tatcgatcag ttccaaactt
     5221 tgaagtggcc aatcacattg gtagctttat ttgtcatcat gttcttgatt tatttaattg
     5281 tccctaatgc tcagttgaag ttgaagacga tcattccggg cacgatcttc gctactgtcg
     5341 gctggatgct gttatcacaa gtgtttagta tctatgctag gtattttgca acgaggatca
     5401 gcggttatca gatcatagga agttttattg tactcatgct ttggcttaat tttgctgcta
     5461 cgatcatcat tttgggcgga atcatcaatg cagttgttca agaatacgtg acgggtcatc
     5521 aaatcaaaaa acgtcgagat ccaagccgaa aatggttcaa taaattgaaa actaaatttt
     5581 ctcataaaaa aaagtagaaa caagagttct tattgtaaat ttatatcagt aatttataat
     5641 aattatgact cttaattaaa gtaggtgccc atatgcttgt ttcttttagt atggttataa
     5701 gattttttct gacaatttta ttatcactga tattaacacc tgtattcaaa atcatttcag
     5761 tacaaacagg tatggtcgac aaaccaaatg aaagaagaat aaataaagtt ccaatgcctt
     5821 cagcgggcgg actgcctatc tttatttcat ttgttatatc gactttattc ttattccgta
     5881 aaattatccc tcaatcgtat attgtaccga tcttgattgc ttctttagta atcatagtca
     5941 caggagtgct cgatgataaa tatgagctga cacctaaaca aaaaagtata gggatcttga
     6001 ttgcttctct tattatttat tttgtagcag atatacgtat tgattccttc acgcttcctt
     6061 ttattggata tatccaacta ggctggctta gttttcccgt aacgattttt tggatcttcg
     6121 gtatcacgaa tgcggtcaat ctgattgacg gtttggatgg gctggcggca gggatttcat
     6181 tgatcggttt aattacgata ggaatcattg gatatttttt cctccatgct tctacagtgt
     6241 atattccagt tgttattttc tgtcttgtag caagtattat cggttttttt ccttacaatt
     6301 tttatccggc aaagatttat ttaggcgata caggagctct attcctaggg tttatgatgg
     6361 ccgtcctttc actacaaggg ctgaagaacg tgacttttgt ttcatcgatt tctttgctga
     6421 tcgtcatggg agtgccggta acagacacgt tttttgctat cattaggaga aaagcaaacc
     6481 gtgtttcttt ttctacagca gataaaaaac atcttcatca ccgactaatc agtttaggct
     6541 ttactcataa aggtgctgtc ctgacgattt atgcgatggc tttgatgttt tctttcacag
     6601 ctatggtcat gaattatact ggaagaatcg gaacgatcat attgattatt gccatgttgt
     6661 ttgctgcaat tttattattt gaattgattg gtctgatcaa tgagaaacat cagtttattt
     6721 taaaaacatt gcgattttta gggaataaag agtaccgtac gcaagtaaaa cagaaatatt
     6781 tgaagaaatg gaaccgtcat atagatgaat aacaaattaa accagccgat ccttattagt
     6841 attgtgacgg caaacagtga taaaattttt cggacactag ataattttat ttcaactgta
     6901 aaagatgaaa cagcagtaaa aatccgaatt tttgacaaca attcacttcc cgagtacaaa
     6961 aaaagactaa cagagtatag gaaatatccc tacatcgata tctattttca tgatgaaaat
     7021 tccggatttg gttatggaca taatcacaat ctgatagcta gcgattctcg ctatggcatt
     7081 atttgtaacc cagatatttt agtggatgaa gagacagtta cagcattggt cttgttattg
     7141 aaagaacatc cggaatgtgc gatgttagca cctaagattc ttaatgaaga tggaacaact
     7201 cagcacctca tccgtgagca gctggctgtt tttgattacg ttttgcgctt tgtgcctttc
     7261 aaatgggtca aaaaaaagtt tgacaaacga ctggctgtgt ttgaatgccg aggattgcct
     7321 actgatgcta atagttatgt acggatgatc tcaggtagtt ttatggtagc tgatattgat
     7381 aaatttaaag agataaaggg gtttgatgag cgtttcttca tgtactttga agacaacgac
     7441 ttgtgtttga gttttgaaaa agcaggttac aagttattgt acacaccctt ttatgcagtt
     7501 atccattttt acgggaaggg tgcgcatcga aatgcaaaac tgtttcgcgt gtttttgact
     7561 tctatgatga aatttttcaa taagtggggg tggcgttttt tctgatgaaa ccagaaatat
     7621 tagtagtcgt tgtgctttat cagcaactat tcagtcaatc tccagcttat ggaccattaa
     7681 gcaaagcact tagcgaaaag caaattcagc ttattattta cgataatagt cctcaaaggc
     7741 agcaaaatga gctgtttgaa aaagaaaata tcgtttatca tcatgatccg caaaatcctg
     7801 gtttagcggt ggcttataat tttgcgttgt ctcaagcaga tgaaggaaca cgttgtctcg
     7861 tcactctgga tcaggatacg aggctagtcg acgattattt tgaactattg cgtaaagtaa
     7921 cctttacaga tgaatgtgtt gctgcagttc cgatgatttt ttctgggagc cgacaagttt
     7981 ctcccctata ttcagatcgt tatatcaatc gtacagctca agcagtagaa gttgaaggcc
     8041 caacagctga acgaatcatg gcaatcaatt caggtgttgc ctggtctgtc aaatttctaa
     8101 aaaagattgg cgggtttaat cctgcttttt ctttagattt tttggatcat tggctgtttt
     8161 ggaaagtgga tcagttggaa aaaactgtgg agatccttcc tgtacgaatg gaacatgatc
     8221 tttcggtttt agactatgaa aaagtttcaa gtaatcgata ccgttctatt ttacaagcag
     8281 aaaatcgatt ttatcatgaa tatgatcaaa ggcatttatc cagtcaccga cgacaattat
     8341 tgttaagaac agctaaacag ttcttgaccg tcaaaaaccg gcatatctgg cggatgactt
     8401 tgcgctcata tgttaagaat tggaaggtgt gaattcgtgc gttcggtttg tatagctacc
     8461 tttaatggag gcagatattt aagagagcag ttagatagta ttttatctca gctttcagca
     8521 gaagatgaag tagtcatttc tgatgatggg tcagtagatg atacatggga tatattaact
     8581 gaatacgcag aaaaagataa gcgaatccag cttttttcgg ggcctcgtca aggattgatc
     8641 gctaattttg gttatgcgat ccagcagacg aaaggagatc taatcttttt ggcagaccaa
     8701 gatgatgttt ggttggagac aaaagtgaaa aagatcactg actattttga gcagaatccg
     8761 gatcagctag tagtagtttc tgatttaatc attgtggaca gcgagctgca agagatccat
     8821 gattcgtatt tttcgtttcg aaattccaaa gaaggatggc ttaataatgt gataagaagt
     8881 cactatattg gtgctggtat ggctttccgg tcttctttaa aacccgatat tttgccgatc
     8941 ccgtcagaag tgccgatgca tgatatgtgg attggtttgc tgggaggaaa gaagactggg
     9001 ttcctaagag aaccgttaac tctgtaccga agacatgatt taaacgcgag tgagattgaa
     9061 acacattcta aattacggca aaaaatttct tggcgtgttc atctgattac ggcgttgatc
     9121 aaacgaaaat ggtttaaacg ttgataaaag atgtttaaat ccttaatatt tccatgacaa
     9181 atataacaat tgatgagata ataacaaagt gaaatttgaa atcaaaaagg agagcagata
     9241 tccatgaagg gaatcattct tgctgggggc agcggaacac gtctgtaccc tttaacaaag
     9301 gcaacatcga aacaattgat gccgatttat gacaaaccaa tgatttatta tccaatgtct
     9361 actctgatgt tggcgggaat caaagaaatt ctgattattt caacgccaca agatacacca
     9421 cgtttcaagg aattatttgg aaacggaaat gatttaggac tgcagatcga atatgcagta
     9481 caagaaagtc cagatggatt ggcacaagcg tttatcattg gagaagaatt tattggagat
     9541 gatagtgtct gcttggtatt aggggacaat atttactatg gtggcggtct gtctaaaatg
     9601 ttgcaacgag cagcttcaaa agatacaggc gcgacagtat ttggctatca tgtcaatgat
     9661 ccagaacgct ttggtgtagt agaatttgat gagaatatgc aagctttgtc gattgaagaa
     9721 aagcctgctc agccaaaatc aaattacgct gtaactggcc tttattttta tgacaatgag
     9781 gtaatttcta tcgctaaagg tatcaaacct tcagaacgtg gagaactaga gattacggat
     9841 gtcaataaag catatttgga taaaggaaaa ctatccgtag aacttatggg acgcggattt
     9901 gcttggcttg atacaggaac acatgaatca ttgcttgaag catctacctt tatcgaaaca
     9961 attgaaaaac gtcagaactt aaaagtggct tgtttggaag aaattgctta ccgtatgggc
    10021 tacatcgata aggaacaatt gctggccctt gcacagccat tgaaaaaaaa tcaatacggt
    10081 caatatttat tgaatttggc cgctgaataa ggagtcctta cagatgaaag taacagatac
    10141 aaaattaaca gatgtcaaaa tcgtagaaat ggacgtcttt ggcgatcatc gcggtttctt
    10201 cacagaaagt tattcaaaag caaaatttgc agaacatgga ttgaaatatg actttgttca
    10261 agacaaccat tcactatcag ctgaagctgg tgtccttcgg gggcttcatt tccaaaaagg
    10321 agaagctgca cagacaaaat tgattcgggt agtcactgga gcagttttag atgtagtagt
    10381 ggatatccgc aaaggtagcc cgacttatgg tcaatgggac ggatttatct tatctgaaca
    10441 taatcatcga cagttgcttg tacctaaagg atatgcccat ggattcgtta ctttgacacc
    10501 aaatgtgaac ttcctttata agtgtgataa ttactataat gcagaagcag atgggggcat
    10561 cgcatttgat gatccagatt tagcgattga ttggccaatt gatccatcaa gagcgattat
    10621 gtcggaaaaa gatcaccgtc atccgacatt aaaagaattt gaagcagaaa atccatttgt
    10681 ttacggagaa atctaagaat taaggagaaa ttttcttatg aaaaacatca ttgttaccgg
    10741 aggtgccgga ttcatcggct ctaatttcgt tcattacgtg gtaaacaatc atccagaagt
    10801 gcatgtcact gttttggata aactgactta tgcaggaaac aaagagaact tggccggact
    10861 tcctagcgat cgcgtagaat tggtcgtagg agatgttgca gatgcagaat tagtggaccg
    10921 tctagtaaaa gaagcggacg cagttgttca ttacgcggca gaatcccata acgataattc
    10981 attaaaagat cctttcccat ttgtacaaac gaatatcatt ggtacgtata cacttttaga
    11041 agcttgccgc aagtatgatg tacgctatca tcacgtatct actgacgagg tttacggcga
    11101 tcttccatta cgtgaagacc tgccaggaca tggggaaggc gaaggagaaa aattcactgc
    11161 ggaaacacca tataatcctt caagtccata ttcttctaca aaagctggtt cagatctgtt
    11221 agtaaaagca tgggtgcgtt cttttggctt acgtgcaacc atttcaaact gttcaaacaa
    11281 ctatggtcct tatcagcata ttgaaaaatt tattcctcgc caagtaacga gtatcttaag
    11341 cggtattcgt ccaaaattat atggcgaagg aaagaatgtt cgtgactgga tccatacgaa
    11401 tgatcattct tcagcagtgt ggctgatttt gacgaaaggc agaattggcg aaacctatct
    11461 gatcggtgca gacggtgaag aagataataa gaccgtgatg gaattgatct tagaaatgat
    11521 gggccaacca aaagacgcat acgaccatgt aaacgaccgc gcagggcacg atttgcgtta
    11581 tgcaatcgat tcgacaaaat tgcgagaaga gctaggttgg cagcctgagt ttacgaactt
    11641 ccgtgatggg ttggctgaca cgatcaaatg gtacgaagaa catgaagact ggtggaaaaa
    11701 agaaaaagag gccgttgaag cagcatatgc caaaaatgga caataataca gaggaaattt
    11761 cactgtatta atggaaaaac acatgtgagg caggcgctcg cctgtctctt ttttaaaata
    11821 aaaatcaagg agcggataac catatgtttt tattaactgg aggaaatgga caattaggta
    11881 cagaattgcg ccatttatta gatgaaaaaa atctgccata tgtttcaaca gatgcaaaag
    11941 acttggatat cacagatgct gagaaaacaa tggcttatat tacagaatta aaacccgatg
    12001 ttattttcca ttgcgcagct tacacagcag tagataaagc agaagaagaa gcaaaagaat
    12061 tagatgagaa aatcaatgtc gatggcacaa gaaatgtagc acttgcagca aaagctgtga
    12121 atgcacaatt cgtctatatc agtacagact atgttttcga tggaaaaaac aaaaatgtag
    12181 aatacgaagt agacgaccaa acgaatccac taaatgaata tggtcgtacg aaattattag
    12241 gcgaaaaagc agtagaagag attttagaag actattatat catacgaact tcttgggttt
    12301 tcgggatcta tggacacaat ttcgtcttca cgatgcaacg cctagcggaa acccgcgaca
    12361 aactgactgt agtaaatgac cagtttgggc gaccaacttg gacacgtact ttggcagaat
    12421 ttatggtata tgtcatcgaa gaaaaagcac catttggtat ttatcatttg tcgaacgaaa
    12481 atagctgttc ttggtatgag ttcgcaaaag aaatattgaa agatacagat gtagaagttg
    12541 cacctgttac gtcagaagaa tttccgcaaa aagcaacacg tccgcagtat tcagtcatga
    12601 gcctgaaaaa gacagaagcg ttaggttttg tcataccaac ttggcaagaa gcacttgcgc
    12661 aaatgctgga agctgccaaa aaaatgaata agtaatttcc tttttatcaa atagaatttg
    12721 gagcatgata tggaaaaaca aacaagaagt gcacgacgaa aagaaaagcc aaaagcgaat
    12781 caggcagatt cgaatttggt gcttgacctg tttctaaaac tccgctatct tattggcgtt
    12841 ttagtcatcg tggtcatcgt tacttttaat ttaaacggaa gttcacttgg tgtttgggat
    12901 aaatatgtgt cgcaaagaga cgatgggaaa aaaactgatg tgatttttgg acagaatcgg
    12961 gaagtgcggt cagacgaatg gctggtgcag acaccttttt acctctcaca agctgaagag
    13021 gacttttcgt tggtgaacga ggattattca ttaaacggac aaaacatgat cattgcgtat
    13081 aattctcctg taaaggatat tacggttatc ggaaaaccat tcaactgggg attctttttc
    13141 ctcggaagag atcgaggact ttccttttat tgggcgatga aattagtcgt catggtcttg
    13201 cttggattcg aagtactgat gatattaacg aagaggaaaa aagctctatc gctaatcggt
    13261 gcatttgcgc tgacattttc tccagcagtc cagtggtggt tcatgcaaca tgtgggcgat
    13321 ttgattttct ttactttggg actgatgatt gctttttatc attatttcta caatcatgat
    13381 aaaaaatggc tacgaacgtt gatgatgctt ttggtggtta ttttcggtat tgggtttatt
    13441 ttagtgattt acccagccca tcaagtgatg ctggcgtact tgctgatttt ttattttgtt
    13501 ggcctgctta tttactatag caagaaagtt tcatgggatc tgttggatgt agtgttgatt
    13561 ggaggggcag ttttatttat tggcggagtc atggttcatt tctggctaac gtcaaaagac
    13621 gcattgttag cttctttgaa taccttatat ccaggaaaac gggtaagtac tggaggaaag
    13681 tggacgattg gcgaattttt ttctttcctt acaaattgga aaattccttt tgaagatatc
    13741 aactattcga ataactctga agtggccctg ttctatcact tttttccaac tgtattccta
    13801 gcaagtccat ttgtgttatt tggcaaaaaa gatagcgaac aaaagttact gggacgtatt
    13861 ttgatgattt tttgtctgtt ttctatcttt tggattaccg ttgggttgcc aaaatggcta
    13921 gcagaaatca ctctgctgtc ttatgttcct ccattaagag ccatccttac ttttagtttt
    13981 gcggcttgtt tattatcgat ctggtttatc gcttatattt ggagagaaga tgttattcct
    14041 atttggtata aattcatttt gttggcagta aatgcatggt cttatttcta tgcgattacg
    14101 cattcgaaaa tggataatta tttttcggat tttgaaacca tcgcagttgc tgtgttggga
    14161 acagtgattt tagcttttgc attatttggc tggagacgct tgttttacct cgcttttgtc
    14221 gccttagtta tcgtatctgg ctttttcgtc aatccagtcg tagaaggtac aggagcgatt
    14281 tttgaaaaaa cactggcaaa agaaatccaa aaaatcgatc aacaagatcc tggtaaagtt
    14341 tggttgtctg aagatgaatt atataacttc accccaacat tgggagtgaa atccttcaat
    14401 acagtacgct tttatccaga tatagctgca tggaagaaaa tcgatccgaa agaagaatac
    14461 gagaagtatt ataatcggta tgctcataca agagctttta tcgctaagga agatacaaag
    14521 ttcattttag atcgtccaga tatgttttcg gtcacgttga attttgaaga cgcagaaaaa
    14581 ttagggatcg actatgtgat cagtaaacga ccattaactg attacaacca attatatgaa
    14641 gcgcagttca cgcagctata tggaccggat aaagacggtt atatgatttt taaagtagaa
    14701 tatccggatt atccagatgt accacaaaca caaactcagg aaaatcagct agtcgtacag
    14761 taataggcta gctgagaata atcctgaaat attagtagaa aggagagtga agataattca
    14821 agcaatcgta caaaaatttg tcaacaaaat gagtgtcggt ttcttgcttt tgttgctgac
    14881 gctagtcttt tttgtttgga cagcaggggt aggttttcca gcaactttgt ttatatataa
    14941 caatagttca ttacttatcg cttttagtgt ggctattttg ctcattttaa atgtttataa
    15001 agcaaaaggc gtcgattttc tttatctagg tgttgcgctt gcccttttca ttttcttttc
    15061 ttactttcgg gaattaagaa atgagtcttt atttggagac ttactgtttc ctttgattat
    15121 tttattttgt ttagtaatta aatggatcga gtggacaaaa tgggatcgcg gtatttatct
    15181 gattgtcttt acggtcttcc tcggaatcac gttatatcgt gtatttacag agatccaagt
    15241 accagaagga caaagcatct gggctccagg caattcgctt tctgatattt ggatcaatac
    15301 gaatacaatc ggaagttctt tgatgactct tgctttattg atcagtggat ttgccagttc
    15361 ttttgaacga tggtatgttc gaatcatcgg tgggatagca gtcgttgcag cctttttagg
    15421 tatttgggtc tgccaatcta gaggtgcatt agtcgcgttg attgtttttg cgatattgga
    15481 tcttttacct aaatcgttga tgcgtgtcat tcgtgcgcca tttattgcct acacggttac
    15541 gattctttta gctttgccga tttcttatct ggcggctgtc tctgaaaaag tcaatttgtt
    15601 taccggtcga gaagatattt ggcataaatt ctatcagacg ctaggagaaa aatctgaaca
    15661 agttttacta ggaatgaaaa catttatctt ccagcgagga aaccaatttt taggaaacca
    15721 caatagttat aattctattt tgaatatcta tggattgata ggatttggga ttgctgcttt
    15781 gctgcttatc ctttttattg gtcgcttaac gttgaaagca gatttatcca acgggcagat
    15841 gacttttatc tgggcctttt ttgcggtgat gatgcagtca tttatggagg atacattgac
    15901 ttcataccca tgggttccga ttatttatat cctgttggct atggccgttc atcgatatga
    15961 tgaaccagaa gttacggagg aattctatgt tatggaagag gcatatgacg atatgactta
    16021 tgatgataca tcttatgagg aacaaccaga tagcgctcat tcttccagga tccaacgcta
    16081 tcattgatag gcacaaaata cgagagaggc tgtgacaacc gtcgcagcct tttttggttt
    16141 attgcaattg tctagcatct tctgtaaaat agaaacgtta gagaaaatct tgtatcatag
    16201 aaaattaaga aagaaataag aacgcagctc ggtaccaaaa atatggtaaa atggcacttg
    16261 actgtaattc actgaaaatc gcttggagga acacaaatgt ttaaagattt gtatttattt
    16321 atgaaggaca tttatcagaa cagaaaatta ctgctacagt tttcgatcaa tgacttcaaa
    16381 tcaagatatg caggatcgtt tttaggaatc ttatgggcgt ttatcaatcc gctatttact
    16441 gtattgattt attggctggt atttggtgtt ggactgaaag cacaaatgat cgatgcaaaa
    16501 tacccattta tcgtatattt agttaccggg atgattccct ggttcttctt ctcagatgca
    16561 tttgcatcaa ctacggttgt ttttcgggaa tatacttatt tagtaaagaa agtcgtattc
    16621 aatattcgta tcttgcccac gacgaaaatc ctatccaatc tgtacaccca tttattcttc
    16681 attctgattg gcatagctgt cgcgcttata aatggtatat atccttcagc aatgtcttta
    16741 cagctgattt attatttatt ttgcatgtgt gctttcttga caggattgac ttggctgacg
    16801 gcatcgatcc aaccattttt acctgatatc atgcaattaa ttacgatcgt gctgcagttg
    16861 atcatgtgga cattaccgat attatggagc cctaatcagt ttgattcagg tcttgtaagc
    16921 atattgaaat taaatccgct gtactatgtt gttcaaggtt atcgtgagtc atttttaagt
    16981 gaagcttggt tctgggagca tggaacatat actttgtatt tctgggtttt cacgttagta
    17041 atgttcgtaa tcggctctgc agtgttccgt agattgaaac cacatttttc agatgtgctg
    17101 taaggagaat agctatgaca gaatatgcaa ttagactaaa agatatcaca aaatcttata
    17161 atatgtacgc aaaaccttcg gatcgatttc gtgaagccct caatcctttt aagaaatcct
    17221 atcatgatgt attttatgcg ttgaaaaacg tgaatgtaga aataaaaaaa ggtgagatga
    17281 tcggtttcgt gggagaaaac ggatcgggaa aatctacaat gttgaaaatc attacgggag
    17341 tactgacacc aacatccggc acgatggaaa tcgaaggaaa aatctcagct ttgctagaac
    17401 taggatcagg attcaatcct gagtattctg gctatgaaaa tatctattta aatggaatgg
    17461 ttctaggatt ttcaagagaa gaagtcgatg aaatggtcga tgatatcatt gaatttgctg
    17521 acatcggcga ccatatttat caacctgtaa aaacctattc aagtgggatg ttcgttcgtc
    17581 tagcttttgc tgtagcgatc aatgtgaatc ctgatatttt gattgtggat gaagcattgg
    17641 cagtaggaga tcttgaattt caactaaaat gtatggaaaa atttacagaa atcaagaact
    17701 cgggaaagac gattttattc gtttctcatg atattagttc gattcgccgt ttttgtgatc
    17761 gttcctattg gttgaaaaat ggaaaagttg tcgaatatgg tgatacgatg gaagtaacga
    17821 cgaattacga aaactttttg aagaaaaaat cagttaaaac agtagatcga aataaaaacc
    17881 aagtagaacg cttcgcagct ccagatatcg tagacgtgac gaaagccgaa ttgttggata
    17941 aagataaaaa tccagtggat atgctggaac aaggagaaaa actatacgtt aaagtaacct
    18001 atgaagtaaa agacgataca atcaaaaatc cagttttagg aattgctctg cgtacagtcg
    18061 acaattttta tgtgtgcgga ctcaatactc tactagatgg tatcaagatc ccttggaaaa
    18121 aaggtgagaa tatcttttat ctggagtatg aaaagttagg tttgttagct ggtgaatatt
    18181 attttgacgt tgcggtcttt gaagaaaatg caacggttcc tttggtatac aagacaaaat
    18241 acatgaactt gtttgtcagc ggttcttata tcggagaagg catcgtggtc ttagaccata
    18301 aatgggaaga agggactcat agcaatgaaa tatgattttg aaatggacct agatgaacaa
    18361 agcagtgtgg gaaaaattgc cgcacagatc aaaccaggaa gtaaagtttt agagtttggt
    18421 cctggaaacg gccgattaac gaaacatttg atcggagcaa aacaatgtga agtgagcatt
    18481 gtcgagctag ataaggaatt gttcgatttt gtcagcgagt ttgcccaaga tggattttat
    18541 ggagatatcg aaagttttga atgggcaaac tattatgctg gacaaacatt tgattacgtt
    18601 ttgtttgccg atgttttaga acatttggta gatcctggaa aaacgttgaa gaaagttaga
    18661 gaatttttga atgaagaagg agaaattcta attacattcc ctaaccttgc gcataattct
    18721 gtcatgattg atttgttcaa taatcaatta ccatgggctt cttatggttt gctagatgaa
    18781 acccataatt ctttttatac tcatgatggc ttccaaaaag tttttgaaaa agcaggatta
    18841 tacatcaata ttgaagatta tttgtactta gctgtaggtg atacagaatt aaaatcaact
    18901 tatgaagaat tgccggaagc tgtacgttat gatttcaaaa tgcgaccatt tggcgaagtc
    18961 taccaatact tcttctcatt gatgaaacat ccagtagcac aaagttccat tgcgcagccg
    19021 caaaattcta attatgtgaa agtattagaa gtaatccagc aaacgaaaca agatgaaata
    19081 attcaacaaa taccattcaa taatttcaca ggggaaaatg agacactaag tttccatgta
    19141 tcagaattag cagaacgaat ggtgtttcgc ttcgcgcagc aacctagctt tatcgaattc
    19201 agcgcagaag ctgctggaga aaaactcaca tttatcgatt caaatgctgt agtcaaaaca
    19261 gtaaatgatt gttatttgtt tgatggtaaa gatttaccag agttcttttt aacggatatt
    19321 gcagggaaag aagtgactat ccattgtcac tatcgtttta tcggagaatt gacaccaaca
    19381 atgaaagaat tgctggaaac aatccgtcca atggctgaag ttacaaagca gctttctgag
    19441 aaaaatgatg aattagaaag agaaaatcat tatcttattg agaataatac aagattagat
    19501 cagacgttga aaacaaccat aaatcgctat tgcacgttgt tggaaagtga tgaatggaca
    19561 atcaaacata gattagggcg acgtaagaaa gaaacatcga aaaaaatcca agaaaaagag
    19621 ctttctctat gtattgacga aaaaatctgg gatgcagaaa caaagatatt gaaaatcatc
    19681 gggtggggaa tcgctaattc ggatcgccaa ccattgtcct ataagttatc agccgaccaa
    19741 tcaccatttt ttgaagcaat tcctgtttta cgagaagaag tcaatcaagc aaaacagcta
    19801 gctgaaggag caaaagcagg atttgaactg cggatccttt gcgagaagga acgttcattc
    19861 ttagttgaag cagtggctca aaatggtcaa tcatggatca ttgaaatgta aataatgatt
    19921 aacttataaa gataaggagt aaacgaatcg tgcaaaaaaa agaaattgcc gtatttatcg
    19981 atcaaatcac tcgtgaacgg gccactggcg atttgcttat tgtgggctgg gcaattgatg
    20041 aagtgaccaa agaaatccct acgataaagg tagaaaaaga aaatgtgatt gctgaagcaa
    20101 cacatgtcgt aagattggat atcaatcatt tatataatct tgatgttaag acccaaagcg
    20161 gttttaaggt acgtttatca gggaaaatga gaggaaaagc aatcttagac ttccaaacag
    20221 caagacatca aaatggcata gctgtgaaat taaacggtaa atatccttat gatgacggca
    20281 ttgaatcttc atgggaaaga aaaaaaagat tactgaaaaa agggataaat tatgcaagga
    20341 ctcatggcgt aaaaaaagca atccgccggg tcaaactgga attgaaacca ggatcgatcg
    20401 actatgcaga gtggatcagt cgtcatgaga aacctgatct gaaagagcaa aggaaagaag
    20461 tacaaggatt tgcctaccgc ccattgattt ccatacttat gcctgtctat aacgttgaaa
    20521 tcaaatggct cgaaaaatgc attgattcgg tacttgatca aacgtatgat cactgggaac
    20581 tatgtatcag tgatgatgct tctactgatc cagctatccg aaagtgtcta gaatcctatc
    20641 aagcaaaaga tgatagaatt aaagtcgttt tccgacaaga aaatggacac atcagtctgg
    20701 caactaattc tgcattgaaa atggcagaag gagagtttat agcgctctta gataatgatg
    20761 acgaattacc tccatttgcc ttatatgaag tggcaaaagt gctaaacgta catccagaac
    20821 tggatttgat ctacagtgac gaagataaaa tcgatgcaga tgggaatcgc tttgacccgc
    20881 acttcaaagc agactggtct cccgatacat tgatgggaaa taattatatc tctcacttag
    20941 gcgtatatcg gacaagcatt gttaaggaac ttggcggttt ccgtaaagga tatgaaggct
    21001 ctcaagatta tgatttggtt cttcgtgtga cagaacaaat tcctgcagaa cacatttacc
    21061 acattgaccg tgtcttgtat cattggcgga cgatcccggg atcaactgca agcaatggag
    21121 aagctaaaag ctacatctat gattctggag tgaaagcatt gacagatgct ctatctagaa
    21181 gaaatatcaa gggaagcgta catcctggca gaatttctgg tttttatgaa atcacttatg
    21241 atgttttgca agaagacttg gtcagtgtga tcattccaac taagaatggc tatgaagatc
    21301 taaaaacttg tgtggattcg atcatcgaaa aaacgtctta tccaaattat gaaatcatta
    21361 ttgcagataa tggaagtaca gatcctaaaa tgcaagaatt atttgcagaa tacaagcatc
    21421 agctgaaaga ccgatttatc gtcgaattaa ttgacattcc attcaattat tcacgtatca
    21481 acaatctggc agcagaaaaa gcaaacggca aatacttttt attcttgaat aacgatacag
    21541 aagtgattga acctgactgg atgacagcga tggtttctta tgcacagttt gatcgaatcg
    21601 gctgtgtagg agcaaaatta ttttatccgg atgatacaac gcagcatgca ggtgtattat
    21661 tagggatcgg tggcgttgca ggacatgcat tgaataacta tgatcgaaca cattgtggtt
    21721 actttggccg attggtcatt gacgtcaatt acttagcagt cactgcagct tgtatgatgg
    21781 tcaaggcagc tgacttcaat gctgtcaatg gatttgatga aatattagaa gtagcattca
    21841 acgatgttga cttgtgcttg aaagtttatg aactgggtcg ttacaatgtc tatgcacatc
    21901 aagcagaatt gtaccatttc gaatctaaat caagaggtta tgaagataca ccagaaaaac
    21961 aaagacgttt tgctggggaa atcaaaaaaa tgcaagataa gtggccttct tatattgctc
    22021 atgacccatt ttataacgat aatctaacta aagaaggaat cggcgatttt tcgttgagac
    22081 ctgattgatt aaaagaactg tattttcatt tttattaatg aaaatacagt tcttttaatt
    22141 taattatatg atttgtcaaa ttaagctaga ataaaatgct tcttgtacat agctcaaaaa
    22201 tatttcgatt ggtgttctgt aattcaatga ttttcttgga atatgattac gttgattgct
    22261 gacactggaa ataaatgtct gattcacttt tctaaaatcc attgatttcg gcagtccatt
    22321 acgacgcaga atcccgttag aattctcgtt taatgggcgt tgagaaggtg ttccagggtc
    22381 cgcaaaatat atatcaatat catgttggtt actaatggct ttccagttag aaaattcttt
    22441 tccacagtca aacgtaatag atttaaagaa gtttttaggg aagcgagaaa accattgatt
    22501 aagggcagtt tcaatatcta atgccttacg gccgttgggt ttaatcgtga taatgacttt
    22561 agataatctt tcaactaaag taatgacggc acttttatga tgaatgccaa cgatcgtatc
    22621 accttcaagg tgaccaaact cagaattgaa atcaggataa ttatcaggtc gatcatggat
    22681 tgagcgctga tactgttgtt ttccccgttt ttcctgatgg ccattgggtt ttcttttacc
    22741 tttcatcggt agtgtgtcaa tatcaaatat tccttta
//
LOCUS       DACECY010000045        21678 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.046, whole genome shotgun sequence.
ACCESSION   DACECY010000045 DACECY010000000
VERSION     DACECY010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.046"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            286..885
                     /locus_tag="IWO38_001858"
     CDS             286..885
                     /locus_tag="IWO38_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313945.1"
                     /translation="MNLKNTNSNKSTLYVISDDYINKLKKVDYRVQNNYREQRLYFKS
                     NLKISNKNEEINYYVPLASAKENQRSLTNKSVFKIYGDREEKDFLGVLHINNMIPVPK
                     DCAKVWTPNSPNVDEKYKMLVIKQAKYLKLHESEINESCELLYNSKLGKLDPSYFKKN
                     RKEVITYQKIMTNVESLEAVSIAESQRNIHVQTQSIDVE"
     gene            997..1221
                     /locus_tag="IWO38_001859"
     CDS             997..1221
                     /locus_tag="IWO38_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313946.1"
                     /translation="MISMARPRKYNTEEERKEAMKKARKKWVEKNQETIAYIRAKSFA
                     KKFIEMSEDEDIELLEKWLEDRKNNGQNDE"
     gene            1573..1809
                     /locus_tag="IWO38_001860"
     CDS             1573..1809
                     /locus_tag="IWO38_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313947.1"
                     /translation="MLSDYKEELKLIENRVLGFVECKDNLDDTLEVLESIIFNTKVLD
                     LKAINGDMTTHIILDEISAKEIGETLIAWANKEL"
     gene            1889..2743
                     /locus_tag="IWO38_001861"
     CDS             1889..2743
                     /locus_tag="IWO38_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0313948.1"
                     /translation="MMKSNFLGYVIAINMFKGGVGKSTLTQLMADILSARGFKVLVID
                     VDPQGTVTKKFQRQYDLKVVGKMTLFEAVINMSFLDCISPVKENLDIVLGSEQMSRFT
                     KVLRDMWRSTGFTDEGEEVFWNLFNYMIGNDNLRDQYDFILFDTIPTVSEFTDNVYVA
                     SDFLVIPTQTEQDSIDNLHSTVTNYQTAVKEVNGDLEILGIVPYLVDTKLKTGLELLS
                     ELQEEYGDLVYSHIVSERGVIKRWGREGLNKSIPHSKTAYSMYVDIVEETLVRLEKAT
                     GENILERV"
     gene            2756..3193
                     /locus_tag="IWO38_001862"
     CDS             2756..3193
                     /locus_tag="IWO38_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313949.1"
                     /translation="MMSKTKKVTSAGSNGSGNPFGIKSGSGGFNIGQQMLKGKAGIKS
                     TGDKRKSVRVPVELYDEVVKDLSYGAKYSLMTEILALFLKEFETHGNSIVTDFGKLKT
                     RPVKISEEAFKVLKVIKFETDQSNTNIIASALTWYLDKRKGKA"
     gene            3513..3956
                     /locus_tag="IWO38_001863"
     CDS             3513..3956
                     /locus_tag="IWO38_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0313950.1"
                     /translation="MQILEDIKIYWKRIIGYTLVLLVVNLFLSMIALVFVNQRKDNVT
                     ELVTFISFFISGIFAPFFEETLMRGIIQNQLELKTNLSTRSIYIIVAILFSLSHLNLY
                     FIPYFMTSLILSFVYDNSDKRLIVPVTIHCFYNLIVILLSVVFYT"
     gene            4101..4424
                     /locus_tag="IWO38_001864"
     CDS             4101..4424
                     /locus_tag="IWO38_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313951.1"
                     /translation="MARSRIAKIEQTKKDKKISKNKTKFFKAEDVKRIAWSVKTFDED
                     RYVKVSMNGLFVQLGNFSNFKTIDELYPEIRNIVDSKGLIVAKRKSRNSGLAYCKNKG
                     IMIPA"
     gene            4842..8471
                     /locus_tag="IWO38_001865"
     CDS             4842..8471
                     /locus_tag="IWO38_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313952.1"
                     /translation="MKKLKLISIALFSLITMSTARQSIVSAEGTSTKAVVEESGDKKV
                     AIDDKVKNEAEKATVVQGADGLMYVVVDENDPEKLLEAESPLKNQQENTGAVEVGVDE
                     KRQEKADKANEKVKNFDDIDYNDISSLQSALNDPKFPFLSEDELNEKLQKTKNTFESE
                     ALMKKTRGYFSQRILELSNNLNDVPKRAKVSRHALVSNRASQVSETRINPPNGYPWVI
                     YLIRSNGYSYTENYAIRRTIDGKEAFCLQAGVQATNTYTGSTSGYLSNSQREMISSIL
                     SAYKELGGWANNDAGKQRWIATQMLVWENSNEKPKSITVTPNWKNGATKWKNDLVARA
                     NAIRVRTKWDSTSKTVDEGDKATFKVTNTYGDHNLHVKSVSGGGKATISNGTLTVDTT
                     GATGNKITVDMIKGSHPSDAHPTTVWSNGSYQKLVTGSFYISTSVTVNVKRKGTVEVL
                     KVDSETKKPLSGATFRFSYSGKTKDVTTDSNGKAKLTERLKAGTVVKVKEIKAPNGYQ
                     LDSSEFSITVKENQNVTTTRTNKKSTGSVEIEKIGDLDGLGLPNVVFTIYNSDNKVVK
                     DNLKTDSNGKIKVDELQFGKYYAVEKQGVTGYDPDGKKYNFEISQDTPFTKPAKVKVT
                     NIYEVSISEKVSDMNESQVLKNNWSADPNDYLQYDITTGNLKKSGERNVQSFIIEGFY
                     DNKNVDVSKSEVYVGSTNVTNSFDINNDTNNGKVIAKAKTSVLTNNDFYNKSYNLRIT
                     MKIKKSTLENTEKQKNLTIVKNSKVTVGSKISKSASSNDVETVLYSRKVTINHIDEKD
                     KHLLKQDIDYKYDGETYEYKPRTDLFDKDGNNYKSDVIHKGKIDGKDIVLNTPYHIPV
                     LSVNVDRIQIDTARAVKNGYLPTKIELSKKEEYEKELEKIVFKVKITDIDNNKVVYDE
                     NFKYKEFNSHMSLDLPTEYLTKNKKINYSVDILLVDNPDKNKFETETKDLRTIGYTSS
                     EKVLTNKDMTNNQVNYSAVVRTVKERENNNPVKEFNESINYKFTDSTKTKTGYGISVD
                     LEPTYRNEIGKQIDMKVYSYPMNELIDSKIDEIYQSGGKETEVKMDRLKNDGKVESGI
                     STQNLSFQFPHMNIERKTGNIYADSQRNDESIKYELIDGGRKLYVPIWCDLGNYSIAI
                     KSSNFGGNYISLDMNQNIDVYAYMYATIDSETKKDDELLLEPVYPESTLPDGWSKNEI
                     DWLNK"
     gene            8531..8893
                     /locus_tag="IWO38_001866"
     CDS             8531..8893
                     /locus_tag="IWO38_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313953.1"
                     /translation="MRVQSYGNSVFDVKREFQDSRRKTKKLIAYFILSAIVFYIVKFV
                     LELNSIVFTDMVHKMLNGEPINFNFDYMLEKAQSNQIVAFIVSIYYFIEKSIFSLKDF
                     FFEMLTKLTTFYNKISKN"
     gene            9127..9549
                     /locus_tag="IWO38_001867"
     CDS             9127..9549
                     /locus_tag="IWO38_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313954.1"
                     /translation="MIYVAFFLDGSDVMLRDMPKMILLMFVGFFLILVIAYYSLNGYT
                     QDSSVKGMQETLRTTAIANRDDSIRVEKGRFALIKSDFEKDFKEKFGKSKNLNNETAD
                     YKFDYLTDGKDGIKAIKVKVESGSKEYQATCILNVAND"
     gene            9561..10976
                     /gene="cpaF"
                     /locus_tag="IWO38_001868"
     CDS             9561..10976
                     /gene="cpaF"
                     /locus_tag="IWO38_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ0313955.1"
                     /translation="MRVVEEKKISPKIEETPKSKINIASIIKKNGIKENSEAIEGNLD
                     FLTKLLEEDSGNYEPPIEDAKLNMIRDDLTRNYSQEVLQALTDDEIRRKLVQVVLKEH
                     GTFFKKDKEVAEYVVRECIGTGIIERIMTNDEITDVGWNGSQLSIETNSNKWLFDGDK
                     LNITQEYIERVISKYAVVNDRAFNSGNPIMDGMFKNVRISATHIDNSPDGSTFSMRVA
                     RPRLALNKENFEDFAPDYILELFEKMMKTKANICISGETGTGKTELQKLLLSFIDNHD
                     RMIMIEDVQETHAKELFPDKDIFSWITTPKKAISDLVKASLRNNPRWIIVSETRGSEA
                     YEMLQAVLSGHYVVTTLHAVNARAIPRRFVNMCASGFKINENAVEEDINRYFDFGVHI
                     KKTVVDGKVIRYLNEIVEFSASEPTTVFKQKFINGKFVIENGSLSEDFKEKMAEKFIT
                     FEFPEDQTNTEESLPEEEEVI"
     gene            10993..11808
                     /locus_tag="IWO38_001869"
     CDS             10993..11808
                     /locus_tag="IWO38_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313956.1"
                     /translation="MTKRKGLMDVSELNAYQVAYGKPLDYRDYLNYVGLPCLFMGGFS
                     FALCYYWWIALICGVIGGVYGFKVIMPKSIKRSYELKSLQERNKFINNMTQILTDDSK
                     TVTRALSIAKQRTKGELREDIQILESRLQGADKFQIAEAFREINNKYYSDVVFTQYFE
                     QLETAIYEGRNNSDTMKQIKSYHNDVKRKTEGFMKIKDGYLQDMKQMMFIVAVFIGAI
                     TFAFGFSTFYEGFARSPIGWVFGSIYFLIMVSFMKSFFKHYFDDEIMSLGVSN"
     gene            11808..12605
                     /locus_tag="IWO38_001870"
     CDS             11808..12605
                     /locus_tag="IWO38_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313957.1"
                     /translation="MKVKALKKKRKSSISPLKITKTEHKSFWIKLLGDVYYQKLREMN
                     NSNIAIIKWQKQRLKKSLIFILVGFLMYLFLGKEWYYLAGGIATSVGFYVWQAKSING
                     IYNQFRFERHLQFSKFTRLLIPYLKQKKDGGNLYGVFAKIVKRLDYDTDKKLLMKLMQ
                     EMTDQPNDIRPFTEYAEQTSGTDMSVLFMSTVYDIRQGSADLDVIEELDRLASEELMA
                     GIDSIIDYKSRRFVFFPTKIAMTSFILVVGFAVGVLFMNLKDINFGQ"
     gene            12623..12985
                     /locus_tag="IWO38_001871"
     CDS             12623..12985
                     /locus_tag="IWO38_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4320 family protein"
                     /protein_id="HAQ0313958.1"
                     /translation="MQEQSVMEIIKWFIGLMMIMAMVSIGLFCVQLQDINTFKQQVNY
                     QIERKGGLTTEAVEAIDKYSATNYDRRFTIESDKLNQKVSYGEEIDYTIKATIPIQIL
                     PLPDVTLSFNGNSVSQIR"
     gene            13157..13378
                     /locus_tag="IWO38_001872"
     CDS             13157..13378
                     /locus_tag="IWO38_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313959.1"
                     /translation="MEAIQENRKDNQETSEKNKKKRKKKVDKFTEMTDKIIEKKGLKP
                     KDFDRQAIKEARQKLYDDFVKENISQLLD"
     gene            13397..13672
                     /locus_tag="IWO38_001873"
     CDS             13397..13672
                     /locus_tag="IWO38_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HAQ0313960.1"
                     /translation="MKNLVISKFAMLPRMDFFDNVSKFMSNMTSKLQGISLSVIIFCI
                     VVTGLMFLFGEGPSRTAKKWLTYIVIGAIIIFGASTIGQTIKSVGGF"
     gene            13781..15607
                     /locus_tag="IWO38_001874"
     CDS             13781..15607
                     /locus_tag="IWO38_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313961.1"
                     /translation="MNQRFEYNFEDVEPKLLKKGKGLKKKNKYTLKQAEALVGWIQHN
                     WSDKTTITLICSDTEEGTKYAFDGIEITPFSIGGQEKLLFPDIEGQILQAPDSMISDP
                     NKLILDINDQYKHEPDVAEPTKESKSKLNLKLPSVVLPFAKKKNKLMNELIEPEETDI
                     YEEELEEQEEIEELVVSSEETEEVEEIETVSENEEETEEIFDFEDEEEVENVQKGDLS
                     TSSQNGLEAVNDPQVPPYISSQTPNYSPQYQAPNVLQPLRKHETVQLVTLKEYCDLGE
                     EIETSISQMVEKLKPESLFKFIGIPSTGLTNTRIDEYRSNHAMLRLAEVKFENLREHY
                     SRQIISFKAEALDRLRTAVDIAWKKPYDLDVKKNKAEELSKMEAEAEAKLNEFVKRQE
                     NNVNDKIKKFEIEQEIDLKNFIARQEADKAVFIATEKDRTQNLIDSKAESINADLLET
                     KDKFIDEEMYKMKIEMNKNLFDGKQKIKSELATELAKANDNVWENALKFIQEIQDEIE
                     AKTPIWAAEIKEFNSLEEQEHERMKQTKELQLKEESIEIKRLEAEMNLKRMEELDHEN
                     KMLKIKLETALSKNELLAMEQDTKQVVNGSNRVGALNLFSKR"
     gene            15620..16336
                     /locus_tag="IWO38_001875"
     CDS             15620..16336
                     /locus_tag="IWO38_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313962.1"
                     /translation="MSVFNNKVMLGNCYVNHIGFYDGGTYRVLFLRLSVFSYSKDGKR
                     HYENINAQISCPLDRNGETTTTSSHPKALMNKFIFEKLDRNLVEGMKISIHGNIRGTE
                     QVLLNSGWKNFGQLSPLEKEEYQKIPKNELVTRNQTYIYIEEVSMPEKEEFRKSQSKV
                     TKEDVEVAEPNQADEILSDDTDPVNDEGEVIEVFEAEIIEEEEEIKSFDKRMYREKPK
                     GYVNFEDMYYDEYSNEPPIN"
     gene            16349..19207
                     /locus_tag="IWO38_001876"
     CDS             16349..19207
                     /locus_tag="IWO38_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ0313963.1"
                     /translation="MKLVQNNEVKEIKNGFVKNSNKILGKLKKVGSFKKNKYHTGKIE
                     GEDLPKSLQDKLNVPLVFIGVFLGSIVFLAITYLLNFLKALLVAIGQSESGLLGVEFD
                     PKFSLAYLKPLGDTKQYFMCAVVAVIGVAYLIYGKLNHESEKNLVYGQKGDSRLSTIK
                     EIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGKGETFVVPL
                     LDNLSRAKIQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLESMSFNPLQLVI
                     DSWANKDYHEASKRANTLTSMLFASGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDC
                     IEKITMYNVAQMLNELGSLFYTDPKTRKEKNALDEYFNTLPQGNQAKIQYGSTSFAGD
                     KAKGSILSTASQGIEMFTSDLFGKLTSKMSIDLKEIGFPKSIQFKVNTNLVGKRISIS
                     FLRKENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLDIRYEEEKSRAWYRIEFPK
                     KQANSHDTSLVTKKFKSGKNYSDLEISTLRLKYTDQPTAVFMVIPDYDPSNHVLASIF
                     ISQLYTELASNCKQTPDKKCFRRVHFLLDEFGNMPAIDNMDGIMTVCLGRNMLFDLVI
                     QSYSQLETRYDKAFKTIKENCQNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLD
                     TDSSVTSSAEQERVITVERASQLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMP
                     YRYQFLAEDFDTQRDINEIDIACEHINLSLQDNQIPYARFIKDLKTRLEYSIINNIPI
                     SEEDYQDYLNLSGSQQTINEEELLKLVNTEVKSQDMEGQVLSEQEMKINIYLKESQKE
                     AIELVKVINGRLADENSADMVIKTINSQILDTPFIQSLINDSIAIHNVQESKDQIMIL
                     EQQLANAYELAVFKTKNSILNNKIIKLKNAMTKLAKAI"
     gene            19258..>21678
                     /locus_tag="IWO38_001877"
     CDS             19258..>21678
                     /locus_tag="IWO38_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313964.1"
                     /translation="MQWLKYKWPIIVSLIIILFMAYPQRVFALPWITDNKEVLKFLTK
                     YQDYLQFTKGGFGSIVVQSLSWGLIRGAYFVCKSAENLVPQSLSLFSFVESSSLGAVY
                     QSVLNTVVVGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALILLMPTLIGSTEK
                     LSGIMFAKTLYNDATSINNQDGGVAWSLIEDGVTDLVYINKNDAYDRLGKDGVQKNAL
                     KEKNFFSYDFSEVITVDTAKDLEDDNKKAEYLQSRLTPDGEGNMQAVKIDDGWMSMFS
                     DSFKGGYYRFTKNTFALTLGLLSLAVAYVFSIFIIVTAILELAFKRVLGVLVFATDIE
                     TGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFVSYLGTGGGSSVNPFIKAVAYVCAVF
                     VLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGNGNGSDS
                     GEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMR
                     DMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDRKDGAKV
                     ENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKSAEELIN
                     QKRQEAKYTPEAMNREEMLRKRVEGRTGTNTDEKEALTKERIQEAKNSNTGLEKLVKE
                     NLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIGVRENLQS
                     SSTSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQG
                     FKTVDVREN"
BASE COUNT         8206 a         2722 c         4207 g         6543 t
ORIGIN      
        1 ttatttttat tcaaaagtgc ccaaaatata ggtatactga attaaggata aatacaaaac
       61 tagttagagg acagttgaaa aaatacaaac aaattagatt gctattttgt taaattggaa
      121 gtagaataat accgttttga tatgtatagt gtgttttacc aataaaatta cataaacgtg
      181 aatatatttt tatgaattaa atgtaaaatg agagcaatta gttttaaaaa agattatagg
      241 ctgttaatct tagcatatat aatccattgg aaagggggag atccaatgaa tctaaagaat
      301 acaaactcaa acaaatctac actatatgta atttcagatg attatataaa taagctgaaa
      361 aaggtggact atcgtgtcca aaacaactat cgagaacaac gattatattt taaatcaaac
      421 ttgaaaattt ctaacaaaaa tgaagaaata aattattatg taccattggc ttcagcaaaa
      481 gaaaatcaac gttctttgac taataaatca gtgtttaaga tttatggtga tcgagaagaa
      541 aaggactttt taggagtact tcatataaat aatatgattc ctgtgccaaa agattgtgca
      601 aaagtatgga cacctaatag cccaaatgtt gatgagaagt ataagatgtt agtaatcaaa
      661 caggcaaaat atttgaaatt acatgagtct gaaataaatg aatcatgcga attactttat
      721 aattctaagt taggtaaact tgatcctagt tactttaaga aaaatcgtaa agaagtaatt
      781 acttatcaaa aaataatgac gaatgtagaa tcattggaag ctgttagtat agcagaatcc
      841 caaagaaata ttcatgtaca aactcaaagt atagatgtag agtaagatag gctaaaccta
      901 tcttatttta ttattctatt ggatattatg atacattgaa ttttaatcga atatgtattt
      961 aaatgttatt atactgtaga agataaaact taaaggatga tttctatggc gagaccgaga
     1021 aaatacaata cagaagaaga aagaaaagaa gcaatgaaaa aggctaggaa aaaatgggtt
     1081 gaaaaaaatc aggaaactat tgcctatata agagccaaat catttgctaa aaaattcatt
     1141 gaaatgtctg aagatgaaga tattgaattg ttggaaaaat ggttagaaga tcggaaaaat
     1201 aacggtcaaa atgatgaata ataaccattg actacaagta gtcaatggtg tagaatggtt
     1261 ttagggatag aaatatccct tttctaaaag tctaatgact acaagtagtc gatagaggga
     1321 tcgggaataa aaacagacga ttatggccta aagaaaggag gaatatgaat attttgagga
     1381 acgatttatt aaatgagcgt tgataaattt cactaaatat tttcttaata tctatgtatt
     1441 gttcaagcgt agaaaaaaat attgaaatta ttacaaaacc gctcataatg gaaaataacg
     1501 aattatatta atgaatcgtt cacttttgac cttttttact agattttttt gtaggacttt
     1561 tagagaattt ttttgttatc tgattataag gaggagctaa agttgataga gaatagagta
     1621 ctggggttcg tagagtgtaa agataattta gacgatactt tagaagtcct tgaaagtatt
     1681 atatttaaca ccaaagttct ggatttgaaa gcaattaatg gtgatatgac aacacatatc
     1741 atattagatg aaattagcgc aaaagagatt ggcgaaactt taatagcatg ggctaataaa
     1801 gagctgtaaa agataatttt aaaataaagt ggccaattga ctacaagtag tctgtatagt
     1861 ataataaatt atcaaaagtg aggtatacat gatgaaatca aattttttgg gatatgtaat
     1921 tgcaatcaac atgtttaaag ggggagttgg taagtcaact cttactcagc taatggctga
     1981 tattctatcg gctagaggct ttaaagtttt agttattgat gttgatccac aaggaacagt
     2041 aactaaaaag tttcaaagac aatatgattt aaaagttgtt gggaaaatga cattgtttga
     2101 agcagtaatt aatatgtctt tcttagactg catttctcct gttaaagaaa acttagatat
     2161 tgtgcttggt tcagaacaaa tgtcaaggtt taccaaagta ttaagagata tgtggagaag
     2221 tactggtttt actgatgaag gtgaagaagt cttttggaat ttgtttaatt atatgatcgg
     2281 taatgataat ttaagagatc aatatgattt tattttgttt gacactattc ctacagtttc
     2341 agaatttaca gataacgttt acgtggcgag tgatttttta gtaattccca cgcaaactga
     2401 gcaagattct atagacaact tgcatagtac ggttactaat tatcaaactg ctgtaaaaga
     2461 agtaaacgga gatttagaaa ttttaggtat tgttccatat ctagtggata ctaaattaaa
     2521 aacaggatta gaacttttat cagaacttca agaagaatat ggagacttag tttattctca
     2581 tattgtttca gaacgaggcg taattaaacg ttggggtaga gaaggactaa ataaatctat
     2641 cccacattct aaaactgctt atagcatgta tgtagacatt gtagaagaaa cgttagtacg
     2701 tttagaaaaa gctacaggag aaaatatttt agaacgtgtt taggaaaggt gttgcatgat
     2761 gtcgaaaact aaaaaagtaa ctagtgccgg cagtaatggt tcgggtaatc cgttcggaat
     2821 aaaaagcggt agtggtggat ttaatatagg acaacaaatg ttaaaaggta aagctggcat
     2881 taagtctact ggtgataaac ggaaatctgt tagggtacca gttgaattat acgatgaagt
     2941 tgttaaagat ttatcatacg gggctaaata ttcattaatg acagaaattt tagctttatt
     3001 tttaaaagaa tttgaaacac atggaaatag tatagttact gattttggca agttgaaaac
     3061 gagaccagta aaaatttctg aagaagcctt taaagttttg aaagttataa aatttgaaac
     3121 agatcaatct aatactaata ttattgcttc agctttgaca tggtatcttg ataaaagaaa
     3181 aggaaaagca taatgaaatg aggtaagact atggatcata aatcaaatta ttcatacaaa
     3241 gaaaattata tttggaagta tgtagtagcc gtttttctac tggtaggtgg aattattgca
     3301 ttggttaaat gggctataat tcctaatgct ttagtattta tcaattggtt tgtccctaat
     3361 ttttggagtt tttttggcta tatattgata atctggttta tgtttaaagt actctctttt
     3421 ttctggtcgc ctgtatattc tttgcttgga aagtggtcgt acctaatttt aatagcgaca
     3481 gctatttata tcattatgca gattaactaa ttatgcagat tttagaagat attaaaattt
     3541 attggaaaag gataataggc tatacattag ttcttcttgt agtaaattta ttcctttcga
     3601 tgatagcgtt ggtctttgtt aatcaaagaa aagacaatgt aactgaatta gtaacgttta
     3661 tttcgttttt cataagtggg atttttgctc ctttctttga agaaacctta atgagaggaa
     3721 ttatccaaaa ccaattggaa ttaaaaacaa atttgtctac aagaagcata tatataattg
     3781 ttgcaatttt gtttagtcta tcccacttaa atttatattt tattccttat tttatgacga
     3841 gtttaatttt gtcttttgtc tatgataatt cagataaaag gctgattgtt ccggttacta
     3901 ttcattgttt ttacaattta attgtaatac tgttaagtgt agttttttat acttgaaaaa
     3961 aatagacgaa aacctaaaaa tacaaggttt ttatctattt tttttatttt gtttttatct
     4021 atcaggttga tagaaattgt cataaatgat ataattaagt atgaactgat ttagtaaaaa
     4081 aattaaagga gtgatagctg ttggcaagat caagaattgc taaaattgaa cagactaaaa
     4141 aggataaaaa aatttctaaa aataaaacaa aattctttaa agctgaagat gttaagagga
     4201 tagcatggtc tgtgaaaaca tttgatgaag atcgttatgt taaagtttca atgaatggtc
     4261 tatttgtaca gcttggtaac ttcagtaatt ttaaaacgat tgatgaactt tatccggaaa
     4321 taagaaatat tgtggatagt aaaggtttaa tcgtggctaa acggaaatca agaaactctg
     4381 gtcttgctta ctgtaaaaat aaagggatca tgattccggc ttaaatcagt gaaattgttc
     4441 tttgaaaact taatactact cgtactgtag caatgttgct atgttacaga taaaagcgta
     4501 aggctaataa gcaaaatagc aaccaagcat actgttattt cctttgccaa gaggtatgta
     4561 aatacgttag caatagaagt gataaggtta gtatttcacg ccaaaaaata caaaagtcga
     4621 tccattgaaa ctactaatta tgaggtggcg taaatggtaa ttattcagaa tttctatcta
     4681 ggaagaacct atgaaaaccg tcctagagat cagaatattg acactaaaaa attcagcaca
     4741 gtaataaatg tgctagatac agggtaactc tttactacaa gaaatgtagt aaagagttgt
     4801 tatgattctt tattacattt ctttctaaag gaagtgtgat aatgaaaaaa ctaaaactga
     4861 ttagcattgc tctattcagc ctaatcacta tgtcaacggc tcgccaatca attgtttcgg
     4921 ctgaaggaac gtctacgaaa gcagtagtag aagagtctgg cgataaaaaa gttgctatag
     4981 acgacaaagt gaaaaacgaa gcggaaaaag cgactgtggt tcaaggggca gacggcttga
     5041 tgtatgtggt cgttgatgaa aatgatcctg aaaaattgct agaagctgaa tctcctttga
     5101 aaaatcaaca agaaaatacc ggcgctgttg aagtcggtgt tgatgaaaag agacaagaaa
     5161 aggctgataa agctaacgaa aaagtgaaaa actttgacga tattgattac aacgatatta
     5221 gttcgttgca gtcagcgttg aatgatccga aattcccttt tttatcggaa gatgaattaa
     5281 atgaaaaatt gcaaaagaca aagaatacat ttgagtctga agcgctaatg aaaaagacaa
     5341 ggggatattt cagtcaaagg attttagagt taagcaataa tttaaatgac gtaccgaaaa
     5401 gagcgaaagt gagtcggcat gctttagtta gtaaccgtgc ttcacaagta agtgaaacac
     5461 gaataaaccc tccaaatggt tatccgtggg tgatttattt aattaggagc aatggttata
     5521 gttatacaga aaattatgct attagaagaa ctattgatgg gaaagaagct ttttgtttac
     5581 aggccggtgt acaagcgacg aatacataca ctggttcaac aagtggttat ctttctaatt
     5641 cgcaaagaga aatgattagt tcgattttat cagcatataa agaattaggc ggttgggcta
     5701 ataatgatgc cggaaaacaa agatggatag caactcaaat gcttgtttgg gaaaacagta
     5761 atgaaaagcc taaaagtata actgttacgc caaattggaa aaacggagct actaagtgga
     5821 aaaatgattt agtggctcgt gcaaatgcta ttagagtaag aacaaaatgg gattctacgt
     5881 ctaagacggt tgatgaggga gataaagcaa cctttaaagt aactaatact tatggagatc
     5941 ataatttgca tgtgaaatct gtatctggtg gcggcaaagc taccataagc aatggtactt
     6001 taactgttga tactacagga gccactggaa acaaaatcac tgtagatatg attaaaggtt
     6061 ctcacccttc agacgcgcac cctacaacgg tatggagtaa tggttcttat caaaagcttg
     6121 ttaccggttc tttttacatt agtacttctg ttacggtaaa tgttaagaga aaaggaactg
     6181 ttgaagtttt aaaagttgat tcagaaacaa aaaaaccatt atcaggagca acatttagat
     6241 ttagctactc aggtaaaaca aaagatgtta ctactgattc gaatggtaaa gcaaaattaa
     6301 ctgaaaggtt gaaagctgga actgtagtaa aagttaagga gataaaagct cctaatggct
     6361 accaactaga ttcaagcgaa ttttcaatta cagttaaaga aaatcaaaat gttactacca
     6421 caagaaccaa taagaaatcc acaggttctg tagaaattga aaagataggc gatttagatg
     6481 gattaggact accgaatgta gtattcacga tttataattc cgataacaaa gttgtaaaag
     6541 ataacttaaa aacagatagt aatgggaaaa taaaagtcga tgaactgcaa tttggtaaat
     6601 attatgcagt agaaaaacaa ggtgttactg gttatgatcc cgatggtaaa aaatacaatt
     6661 ttgaaataag tcaagatact ccttttacaa aaccggctaa agtgaaagta acaaatattt
     6721 atgaagtatc cataagcgaa aaagtatcgg acatgaatga aagtcaggta ttaaaaaata
     6781 attggtctgc tgatccaaac gattatcttc aatatgacat tacaactgga aatctaaaaa
     6841 aatcgggaga aagaaacgtc caaagtttca ttatcgaagg gttttacgac aataagaatg
     6901 tagatgtgtc aaaaagtgaa gtatatgttg gatcaacaaa tgtaacaaac tcttttgaca
     6961 ttaataacga tactaacaat ggaaaagtaa ttgctaaggc gaagacaagc gtattaacga
     7021 ataatgattt ttataataag tcctataatt tacggatcac tatgaaaatt aaaaaatcta
     7081 ctcttgaaaa cactgaaaag caaaaaaatt taacgattgt taaaaatagt aaagttactg
     7141 tcggttcaaa aatctctaaa tctgctagct ctaatgatgt agaaaccgtt ttatattcaa
     7201 gaaaagttac tatcaatcat attgatgaaa aagacaaaca ccttttgaaa caagatattg
     7261 attataaata cgatggggaa acgtatgaat ataaacctag aaccgattta tttgataaag
     7321 atggaaacaa ttataaatct gatgtaatcc ataaagggaa aattgatgga aaagatatag
     7381 ttttaaacac tccctatcat attccggtac tttctgtgaa tgtggatcgg attcaaattg
     7441 atacggctag agctgttaaa aatggctatt tacctacaaa aatagaacta agtaaaaaag
     7501 aagaatatga aaaagaatta gaaaaaattg tattcaaagt taaaattaca gatatagata
     7561 acaataaagt tgtttatgat gaaaacttta aatacaaaga atttaatagt catatgtcgc
     7621 ttgatttacc tacagaatat ttaacaaaga ataaaaaaat caattatagt gttgatattc
     7681 ttttagtgga taatccagat aaaaacaagt tcgaaactga aactaaagat ttaagaacga
     7741 tcggttatac ttcttctgaa aaagttttaa caaacaaaga tatgacaaat aaccaagtca
     7801 actattcagc agtagttaga acagtgaaag aaagagagaa taataatccg gtcaaagagt
     7861 tcaatgagag tatcaattat aaatttactg atagcactaa aacaaaaaca ggatatggta
     7921 tatcagttga tttagagcca acatatagaa atgaaattgg gaaacaaata gatatgaaag
     7981 tctatagtta tccgatgaat gaattgattg acagcaaaat tgacgaaatc tatcaaagtg
     8041 ggggaaaaga aacagaagtt aagatggatc gcttaaagaa tgatggtaaa gttgagtcgg
     8101 gtattagtac tcaaaatctt tcattccaat ttccacatat gaatatcgaa agaaaaactg
     8161 gaaatatata tgctgacagt caaagaaatg atgaatctat taagtatgaa ttgatcgatg
     8221 gcggtagaaa attatatgtg cctatttggt gtgatttggg aaattattct attgctatca
     8281 aaagtagtaa ctttggtggt aactatatct cgttggatat gaaccagaac atagatgttt
     8341 atgcttatat gtatgcaacg atagatagtg aaacaaaaaa agatgatgaa ttactgcttg
     8401 aacctgttta tccagagtct actctaccag atggttggag caaaaatgag attgattggc
     8461 tgaataaata agtttgaaaa gtttttatct atctgataga attaaataat gatataggga
     8521 tggtgataga atgagggttc aaagctatgg taacagtgtc tttgatgtta aaagagaatt
     8581 tcaagattct agaagaaaaa ccaaaaagct gatagcttac ttcattctat cagctatcgt
     8641 tttctatatt gtcaagtttg ttttggaatt aaactcaata gtattcacag atatggttca
     8701 taaaatgtta aatggagaac ctattaattt caactttgat tacatgcttg aaaaagcaca
     8761 aagcaatcaa attgtggctt ttatcgtgag tatctattat tttattgaaa aatctatttt
     8821 ttctttaaaa gattttttct ttgaaatgct taccaaacta acaacgtttt acaataaaat
     8881 ttcaaaaaat taaggaggaa gtttgaatgt tagatcaaaa tacagacaga agttggtgga
     8941 tgattggtgc cgtgatcgta ggtgccatct taatcgggac agctaaagta gcttttcccg
     9001 aagtgtttaa tatcgtcatt acatttttca aaaacatgat ccctacaaac ttaggatagt
     9061 tccactaaaa aattcacgta gtttgatgcg tagaaaaata ataaagaatg aaaaaggcta
     9121 cataaagtga tttatgtagc cttttttttg gatggaagtg atgtaatgct aagagatatg
     9181 cccaaaatga ttcttttaat gtttgttggg ttctttttaa ttttagtgat tgcgtactat
     9241 tcattaaatg gctatacaca agattcgtct gtgaaaggta tgcaagaaac gttaagaact
     9301 acagcaatag caaacaggga tgatagtatt cgagtagaaa aaggacgatt tgctttaatt
     9361 aagtctgatt ttgaaaaaga ttttaaagaa aaatttggga aaagtaaaaa tctaaataat
     9421 gaaaccgctg attataaatt tgattatctt acagatggga aggacggtat taaagcaatt
     9481 aaagtgaagg ttgaaagtgg atcaaaagaa tatcaagcaa cttgtatttt aaatgttgca
     9541 aatgattaga ggtgtgaaat ttgcgtgttg tagaggaaaa gaagatttct ccaaaaatcg
     9601 aggaaactcc taaaagtaaa attaatatcg caagtattat taaaaaaaat ggaattaaag
     9661 aaaactctga agcgatagaa ggaaaccttg attttttaac taagctttta gaagaagata
     9721 gtggaaatta tgaaccacca attgaagatg caaaattaaa tatgattcgt gatgatttaa
     9781 ccagaaacta ttcccaagaa gttttacaag ctttaactga tgatgaaatt aggcgaaaac
     9841 ttgtacaagt tgttttgaaa gaacatggta ctttctttaa aaaagataaa gaggttgcag
     9901 aatatgtagt acgtgaatgt attggaacag ggattattga gcgaattatg accaacgatg
     9961 aaattacaga tgttggttgg aatggctctc aacttagcat tgaaactaac tctaacaaat
    10021 ggttgtttga cggtgataaa ttaaatatca cgcaagagta tattgaacgt gttatttcta
    10081 aatatgctgt tgtaaatgat cgggctttta atagtggtaa tcctatcatg gatggaatgt
    10141 ttaaaaatgt tcgtatatca gctacacaca tagataattc accagatggc tctacgtttt
    10201 ctatgcgtgt ggcaaggcct agattagcat tgaataaaga aaactttgaa gattttgctc
    10261 ctgattatat tttggagcta tttgagaaaa tgatgaaaac aaaagcaaat atctgtatat
    10321 caggggagac aggtacaggt aaaacggaac ttcaaaaatt actgctaagt tttattgata
    10381 atcatgatcg tatgattatg attgaggacg ttcaagaaac tcacgctaaa gaattatttc
    10441 cggataaaga tattttctct tggatcacta ctcccaaaaa agcaatttca gatttagtaa
    10501 aagcttctct tagaaataat ccacgatgga tcattgtttc agaaacacgt ggttctgaag
    10561 cttatgaaat gcttcaagcg gttttgtcag gtcactatgt tgtaacgaca cttcatgcgg
    10621 ttaatgcacg tgcgatacct agacgttttg taaatatgtg tgcaagtggt tttaaaataa
    10681 acgaaaatgc ggttgaggaa gacataaatc gttatttcga ttttggtgtc catatcaaaa
    10741 aaacagtggt tgatggtaaa gtgatccgtt atttgaatga aattgttgaa ttttctgcta
    10801 gtgaaccgac tacagttttt aagcaaaaat ttatcaatgg aaagtttgtc attgaaaatg
    10861 gtagtctttc cgaagatttt aaagaaaaaa tggctgaaaa atttattacg tttgaatttc
    10921 ctgaagatca aactaataca gaagaatcac ttcctgaaga agaagaggtg atttaagttg
    10981 aaagtacagg ctatgacaaa gcgaaaaggt ctcatggatg tttcggaact aaacgcctat
    11041 caagtagcgt atggtaaacc gttagattat agagattatt tgaactatgt tggattacct
    11101 tgtttgttta tgggtggctt ttcttttgct ttatgttact actggtggat tgctttgatt
    11161 tgtggagtaa ttggaggcgt atatggcttt aaagttataa tgcctaaaag tattaaacgc
    11221 tcttatgaat taaagagttt acaggaaaga aataaattta ttaacaacat gactcaaatt
    11281 ttgaccgatg atagtaaaac agttactaga gcgttatcta tagcaaaaca gcgaacaaaa
    11341 ggtgaattaa gagaagatat tcaaatctta gaatcacgtt tacaaggtgc tgataaattt
    11401 cagattgctg aagcttttcg tgaaatcaac aataaatatt atagtgatgt tgtttttaca
    11461 caatactttg agcaattgga aacagctatt tacgaaggta gaaataattc agatactatg
    11521 aagcaaataa agagctatca taatgatgtg aaacgtaaaa cagaaggttt tatgaaaatc
    11581 aaagacggct atcttcaaga tatgaagcaa atgatgttta ttgtagctgt ttttattgga
    11641 gctataactt ttgcttttgg tttttctact ttctatgaag gttttgctcg atcacctatc
    11701 ggatgggtat ttggatctat ttactttttg attatggtta gcttcatgaa gtcatttttc
    11761 aaacattatt ttgatgatga aataatgagt ttgggggtaa gtaattaatg aaagttaagg
    11821 ctttaaaaaa gaaaagaaaa tcaagtatta gccccttgaa aattacgaaa acagaacata
    11881 aaagcttttg gattaaatta ctaggtgatg tttattatca aaaattacgt gaaatgaaca
    11941 attccaatat tgcgattatt aagtggcaaa aacaacggtt aaaaaagtca ttgattttta
    12001 tcctagtcgg attcttaatg tatctatttt tagggaaaga atggtattac ttagccggtg
    12061 gaattgctac ttcagtaggt ttttatgttt ggcaagcaaa atcaatcaat ggtatctata
    12121 atcaatttcg gtttgaacga cacttgcaat ttagtaaatt tacacggcta ttgatcccct
    12181 atctgaaaca aaaaaaagat ggcggaaatt tatatggtgt ttttgcaaaa atcgtaaaaa
    12241 gactagatta tgataccgat aaaaagctat taatgaagct tatgcaagaa atgacagatc
    12301 agcctaacga tattagacct tttacggaat atgcagaaca gacttctgga acagacatgt
    12361 ccgtattatt tatgtctaca gtttatgata ttcgacaagg ttcggcagat ttggatgtta
    12421 ttgaagagtt ggatcgttta gcttctgaag agttaatggc cggaatcgat tcaatcattg
    12481 actataagtc gaggcgtttt gtcttctttc ctacaaaaat tgcaatgact tcatttattt
    12541 tagttgtcgg ttttgcagta ggcgttcttt tcatgaacct aaaagatatt aactttgggc
    12601 aataaaggaa aggagaaagt caatgcaaga acaatccgtt atggaaatca ttaaatggtt
    12661 tataggttta atgatgatta tggcaatggt ttctatagga cttttttgtg ttcaattaca
    12721 agatattaat actttcaagc aacaagtaaa ttatcaaatt gaacgaaaag gaggtctgac
    12781 gacagaagct gttgaagcaa tcgacaaata ttcggctact aattatgata gacgttttac
    12841 aattgaaagc gataagttaa atcagaaagt atcttatgga gaagaaattg attatacgat
    12901 caaagcaact attccaattc aaattttacc gcttcctgat gtaactttat cgtttaacgg
    12961 aaatagtgtt tctcaaataa gatgagagga aggataaaat gcaagagaat aatgtttcca
    13021 gaataattta tttgattggc ggattgataa ttttatcctt agctattgcc ggtcttgtag
    13081 agcattggcc ggaaataaag ttattaataa gaaactttta cgacaataaa atgattaaaa
    13141 tgtaggaggt atagatttgg aagcaattca agaaaatcgt aaggataatc aagaaacaag
    13201 tgaaaaaaac aaaaaaaaaa gaaaaaagaa agtagataaa tttacggaaa tgaccgataa
    13261 aatcattgaa aagaaaggac ttaaacctaa agactttgac cgtcaagcaa tcaaagaagc
    13321 acgacaaaaa ctgtatgatg attttgttaa agaaaacata tcacagcttt tagactaaca
    13381 tttaggggga aataaaatga aaaatttagt tatcagcaag tttgcaatgt taccaagaat
    13441 ggattttttc gataatgtaa gtaagtttat gtcaaatatg acttcaaaac tacaaggaat
    13501 ttcactatct gtaattatct tttgtattgt ggttactggt ttgatgtttc ttttcggaga
    13561 agggccaagt cgtactgcta aaaaatggtt aacttatatc gtgattgggg caataattat
    13621 cttcggagct tcaactatcg gacaaacgat caagtccgtt ggtggtttct aaggggaagc
    13681 ttcttagaac taaaaaattc gcaagtattg catgaaagag cattgagtga ttaatgctct
    13741 tttttatttt gaaaaactat aaactaagga gatttaaact atgaaccaac gattcgaata
    13801 taattttgaa gatgtagaac ctaagttact gaaaaaagga aaaggattaa aaaagaaaaa
    13861 taaatatact ctcaaacaag cagaagcatt agtaggatgg attcaacaca attggtcaga
    13921 caagacaaca attaccttga tctgttcaga cacagaagaa ggaacaaagt atgcttttga
    13981 tggaattgaa attactccat tttcgattgg cggacaagaa aaattattgt ttcctgatat
    14041 tgaaggacaa attttacaag cgccggacag catgatttcc gatcctaata aactgatttt
    14101 ggatattaac gatcaataca aacacgaacc agacgtagca gaaccaacaa aagaatcaaa
    14161 gtcaaaatta aatttgaagc tcccttcagt agtattacca tttgctaaga agaaaaataa
    14221 acttatgaac gaattgattg aaccagaaga aacggatatt tatgaagaag aattagaaga
    14281 acaagaagag atcgaagaat tagttgtgtc ttctgaagaa acagaagaag tagaagaaat
    14341 tgaaactgtt tctgaaaatg aagaagaaac agaagaaatc tttgactttg aagatgaaga
    14401 agaagtggaa aatgtacaga aaggcgatct atctacaagt agtcaaaatg ggttggaagc
    14461 tgtaaatgat ccacaagttc caccatatat atcttcacaa actcccaatt actctccaca
    14521 gtatcaagcg cctaatgtct tacaaccatt aagaaaacac gaaacagtgc agttagttac
    14581 gcttaaagag tactgtgatt tgggagaaga aattgaaact tcaatttctc aaatggtcga
    14641 aaagttgaaa ccagaatctt tattcaaatt catagggatt ccttctacag gtcttacaaa
    14701 tactcgtatt gatgaatata gaagtaacca tgctatgttg cgattagctg aagtaaaatt
    14761 tgaaaattta agagagcatt attcacggca aattatttct tttaaagcag aagccctaga
    14821 tcgtttaaga actgcggtag atattgcttg gaagaagcct tacgatttag atgtgaagaa
    14881 aaacaaggcc gaagagttat caaaaatgga agcagaagca gaagctaaat taaatgagtt
    14941 cgtgaaacgt caggaaaaca atgtaaatga taaaatcaaa aagtttgaga tcgaacaaga
    15001 aatagattta aaaaacttta ttgcaagaca agaggcagat aaagcggtat ttattgctac
    15061 agagaaagat cggactcaaa atttaattga ttctaaagcg gaatcaatca atgcagattt
    15121 acttgaaaca aaagataaat ttattgatga agaaatgtac aaaatgaaaa ttgaaatgaa
    15181 caaaaattta tttgatggca agcaaaaaat taaatcagaa ttggctactg aactcgctaa
    15241 ggcaaatgat aatgtttggg agaatgcgct gaaatttatt caagaaattc aagatgaaat
    15301 tgaagctaaa acaccaattt gggctgctga aatcaaggag tttaatagtt tagaagaaca
    15361 agaacatgaa cgtatgaagc aaactaaaga acttcaatta aaagaggaaa gtattgaaat
    15421 caagcgtttg gaagctgaaa tgaatttgaa gcgtatggaa gaattagatc atgaaaataa
    15481 aatgcttaaa attaaacttg aaactgccct ttcaaaaaat gagttattag ctatggaaca
    15541 agatacaaag caagtggtga atggatcaaa cagagttggt gcacttaatt tatttagtaa
    15601 acgttaaagg agaacggata tgagtgtttt taataataaa gttatgttag ggaactgtta
    15661 tgtgaaccac attggttttt acgatggagg aacatacaga gttttattct tacgtttatc
    15721 tgtttttagt tactcaaaag atggaaagag gcattatgaa aatattaatg cacaaatttc
    15781 ttgtccttta gatagaaatg gggaaaccac gactacaagt agtcacccta aagccttgat
    15841 gaataaattt atctttgaaa agttggatcg caatttggtt gaggggatga agataagtat
    15901 tcatggaaat attcgtggaa cagaacaagt gcttcttaac agtggttgga aaaactttgg
    15961 tcaattatcg cctttagaaa aagaagaata tcaaaaaatt cctaaaaatg aattagtaac
    16021 aagaaatcaa acatatatct atatagaaga ggtaagtatg ccagaaaaag aagaatttag
    16081 aaagtctcaa tctaaggtaa caaaagaaga tgtggaagtt gcagaaccga atcaagcaga
    16141 tgaaatttta tccgatgata ctgatccagt gaatgatgaa ggagaggtta ttgaagtttt
    16201 tgaagctgaa attatcgaag aggaagaaga aattaaaagt tttgataaaa gaatgtatcg
    16261 agagaaacca aaaggttatg tgaattttga agatatgtat tatgacgaat attctaatga
    16321 accaccgatc aattagtggg ggtcaaatat gaagctagta caaaacaatg aagtgaaaga
    16381 aataaaaaac ggctttgtta aaaactcgaa caaaattttg ggaaagctga aaaaagtagg
    16441 tagtttcaaa aaaaataaat atcatactgg aaaaattgag ggagaagatt taccaaaaag
    16501 tttacaagat aaattgaatg ttccgcttgt atttataggt gtttttttag gttcaatagt
    16561 ttttctagca ataacgtact tattgaactt tttaaaagct ttgttagttg caatcggaca
    16621 gtcagaaagt gggctacttg gtgtagagtt cgatcctaag tttagtttgg catatctaaa
    16681 accacttggg gatacgaaac aatactttat gtgtgctgtg gttgcggtaa taggagtcgc
    16741 ttatttaata tatgggaaat taaatcatga aagtgagaaa aatcttgttt acggtcaaaa
    16801 aggagattca agactttcaa ctatcaaaga aattaagaga gattaccatg agataccaga
    16861 aaaaacagaa gtgtatgatg gtattggtgg tgtgccaatt agtcactatc aagataaata
    16921 ttatattgat agagacacag taaataccgt tgttttggga gcttcacgat ccggtaaagg
    16981 ggaaacattt gttgttcctt tattagataa tcttagccga gcaaaaatac aaagtaatat
    17041 ggtcgtgaat gatccgaaag gagaattgtt tagcgcttca aaggaaacac ttgaaaaacg
    17101 aggttatcgt gtattagtgc taaatattga tgatccactt gaatcaatga gttttaatcc
    17161 tttacaacta gtaattgata gttgggctaa caaggactat cacgaagcta gtaagcgagc
    17221 gaatacttta acttcaatgc tctttgcttc aggaatgggt acagataatg agtttttcta
    17281 taaatcagct aaatcagcgg tcaatgcgat tattttaacg attgtagaac actgttttaa
    17341 caatgattgc attgaaaaaa ttacaatgta caacgtggct caaatgctga acgaattagg
    17401 aagcttattt tacactgatc caaagactag aaaagaaaaa aatgctttag atgaatattt
    17461 caatacttta ccacaaggga atcaggccaa gattcagtat ggttctactt catttgccgg
    17521 tgataaagcc aaaggttcga ttttatctac tgcttcacaa ggtattgaaa tgtttacaag
    17581 tgatttgttc gggaagttaa caagtaaaat gtctattgat ttgaaagaga taggctttcc
    17641 taagtccata caatttaaag tcaatacgaa tttagttgga aagaggatta gtatttcatt
    17701 tttgagaaaa gaaaatgatg taattagatt aattaagaag tatcgagtga aagtaaaagc
    17761 tttagggcta tgtgttttaa attttaatga atatcttcag gatggagata tgttggatat
    17821 acgatatgaa gaagagaaat cgagagcatg gtatcgtatt gaatttccca aaaaacaagc
    17881 aaatagtcat gatacttctt tagtcactaa aaaattcaag tcaggtaaaa attattctga
    17941 cttagaaatc agcaccttac gattaaaata cacggatcag ccaactgctg tgttcatggt
    18001 tattccagat tatgatccgt caaatcacgt acttgcaagt atttttatca gtcaacttta
    18061 tacagaatta gcaagtaatt gtaagcaaac accggataaa aaatgtttta ggcgagttca
    18121 tttcttgtta gatgaatttg ggaatatgcc ggctatagac aatatggatg ggattatgac
    18181 ggtatgtcta ggaagaaata tgctttttga cttagtgatt caatcctata gtcagctaga
    18241 aacaagatat gataaagctt ttaaaacaat taaagaaaat tgtcagaatc atattttaat
    18301 catgtcgaac gatgaggaaa cgatagaaga gatttcaaaa aaatgtggtc ataagacaac
    18361 aataaataga agtgctagtt ctaaacattt agatactgat agctcggtca ctagttcggc
    18421 agagcaagag cgagtaatta cagttgagcg tgctagtcaa ttgattgaag gggaacaaat
    18481 tattttaaga aaccttcata gacaagatac aaaaagaaat aagattagac catttcctat
    18541 ttttaatact aaagaaacaa atatgccgta tagataccaa tttttagcag aagactttga
    18601 tactcaaaga gatattaatg agattgatat tgcatgtgaa catatcaatc tatctttaca
    18661 agataaccaa attccatatg caaggtttat caaagattta aaaacacgtt tggagtattc
    18721 aattattaat aatattccta ttagtgaaga agactatcag gattatctaa atttatcagg
    18781 atcacaacaa acaattaatg aagaagagct gttaaaactg gtaaatacgg aagtaaaaag
    18841 tcaagatatg gaaggtcaag ttttatcaga acaagaaatg aaaattaata tttatctaaa
    18901 ggaaagtcaa aaggaagcaa tagaattagt aaaagtaatt aatggaaggt tagctgatga
    18961 aaattcagca gatatggtta taaaaaccat taattcacaa atccttgata cgcctttcat
    19021 tcaatcttta atcaatgatt ctatagctat tcataatgtt caagagagta aggatcaaat
    19081 tatgatccta gaacaacaat tagctaatgc ttatgagtta gcagtgttta aaacgaaaaa
    19141 ttcaatactg aataataaaa ttatcaagtt gaaaaatgct atgactaagc tagcaaaagc
    19201 aatttagaga gaagggaaga aatattgact gaatctatta attggagaaa actaaatgtg
    19261 caatggttaa agtataaatg gccaatcata gtttccctaa taatcattct atttatggcc
    19321 tatccgcaac gagtttttgc gttgccgtgg attacagaca ataaagaggt attaaaattt
    19381 ttaacaaaat atcaagatta tcttcagttt actaaaggtg gttttggaag cattgtcgtt
    19441 caaagtttat cgtgggggct gattagaggt gcatattttg tttgtaaatc agcagaaaac
    19501 cttgttccac aatcgctaag tttgtttagc tttgttgaaa gcagtagttt aggtgctgtt
    19561 tatcaatctg tattaaacac ggtggttgta ggattaatga tcctttcatt gatgtttgtt
    19621 ggttacaaga tgattactgg aaaaggaaca gtggatttaa aatcggttgg aatgaacgta
    19681 gtgatgtcta tagcattaat acttttaatg ccgactctga ttggatcaac agaaaaactt
    19741 tcaggaataa tgtttgcaaa gactctttat aatgacgcaa ctagtatcaa taatcaagat
    19801 ggcggtgtag catggtcatt gattgaggac ggtgtaactg atttagtata tatcaataaa
    19861 aacgatgctt atgatcggtt gggtaaagat ggcgtacaaa aaaatgcgtt aaaagaaaag
    19921 aatttctttt cctacgattt cagtgaagta atcacagttg atacagctaa agatttagaa
    19981 gatgataata aaaaagctga atatcttcaa tctaggttga caccagatgg cgaaggaaat
    20041 atgcaagccg ttaagattga tgatggttgg atgtcgatgt tctctgatag tttcaaagga
    20101 gggtactaca ggtttactaa aaatactttt gcattgactt taggattact ttcgttagct
    20161 gtagcctatg tttttagtat ttttatcata gtcaccgcta ttttagaact ggcatttaag
    20221 cgtgtgttag gggttcttgt ttttgcaaca gatattgaga caggacagcg ttcaaaagtt
    20281 gttctatcag atattctaca gtgctatttg actgtaggct ttcaaggatt tggattatcc
    20341 atgtttgcaa tgtttgtttc ctatttagga acaggcggag ggtcttccgt taatccgttt
    20401 attaaggctg tagcttatgt atgtgccgtt tttgttttaa tcaaaggcag tggaacagtc
    20461 atgagatact ttggtgttga tattggactc aaagaaggct atgggcaact tgcttctgct
    20521 tttggaatgg gtgcaatgct gtttagaaaa ggatcaaatg gatttaatcg tgccaaagga
    20581 agcggaaacg gaaatggatc agacagcgga gaaggagaag atcgaaaacc agaaaagaat
    20641 ttcggtgaaa ctctatcaaa aaaagctggc aaaactggaa gagctttggg atatgcccat
    20701 gaacgtggat tatcaggttt agcttcagat ggagctacaa tggctagtga aagagcaaca
    20761 aaaccattta agagtatgcg tgatatggct aatgatacta agaataagtt caaagaaggt
    20821 ttagatgatg gtacagtgtc cgctattaat aaaaatagca aacctatgtt agctaaaaat
    20881 aaagaagatg aaactggaaa atatgctgat tccatgccaa cacgtctttc ggatagaaaa
    20941 gatggtgcta aggttgaaaa tgccgataga attatgtcta gttctgaacg tatgcgtgaa
    21001 gcaatgaaaa ataatgctga atcgaataat aatcctgtaa gtgcaattca gcaaaaagtt
    21061 caacaagata ttgaagaacg taaaaatgct atgcacggtc aagctaagtc tgctgaagaa
    21121 ttaattaatc agaagcgaca agaagctaaa tacacgcctg aagcaatgaa tcgtgaggaa
    21181 atgttaagga aacgtgtaga agggcgtaca ggaactaata cggacgaaaa agaagcgttg
    21241 accaaagaaa gaatccaaga agctaaaaac tcaaataccg gattagaaaa attggtcaaa
    21301 gaaaatctac aaaattctac aagtagtcaa ggtggtcgat cagtagatgt ccgagaaaat
    21361 attcaaggtt cattgaatgg tcatgtcgga cggaatgttg atgtaagaga gaatttacaa
    21421 agttcttcaa atggtcaagg tactaagaca attggtgttc gagaaaatct gcaaagtagc
    21481 agtacaagtc aaccaaaaac agtagatgtt cgagaaaatg tacaaaattc tacaagtagt
    21541 caagggttta aatcagtgga tgttcgagag aatgtgcaag gtagcagtgc aagtcaacca
    21601 aaaacagtag atgtccgaga aaatgtacaa agttctacaa gtagtcaagg attcaaaaca
    21661 gtagatgttc gggagaac
//
LOCUS       DACECY010000046        21393 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.047, whole genome shotgun sequence.
ACCESSION   DACECY010000046 DACECY010000000
VERSION     DACECY010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.047"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            185..1183
                     /locus_tag="IWO38_001878"
     CDS             185..1183
                     /locus_tag="IWO38_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0313965.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="IWO38_001879"
     CDS             1295..1804
                     /locus_tag="IWO38_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ0313966.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="IWO38_001880"
     CDS             complement(1834..2493)
                     /locus_tag="IWO38_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ0313967.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="IWO38_001881"
     CDS             complement(2495..3259)
                     /locus_tag="IWO38_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ0313968.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="IWO38_001882"
     CDS             complement(3267..3944)
                     /locus_tag="IWO38_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ0313969.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="IWO38_001883"
     CDS             complement(3981..5366)
                     /locus_tag="IWO38_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ0313970.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="IWO38_001884"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IWO38_001884"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="IWO38_001885"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="IWO38_001885"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ0313971.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="IWO38_001886"
     CDS             6305..7162
                     /locus_tag="IWO38_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ0313972.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="IWO38_001887"
     CDS             complement(7243..7578)
                     /locus_tag="IWO38_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313973.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="IWO38_001888"
     CDS             complement(7645..9015)
                     /locus_tag="IWO38_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ0313974.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="IWO38_001889"
     CDS             complement(9093..9629)
                     /locus_tag="IWO38_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0313975.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            9938..11419
                     /locus_tag="IWO38_001890"
     CDS             9938..11419
                     /locus_tag="IWO38_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0313976.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTTKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHKLYMKKGYENKTKRM"
     gene            complement(11522..12229)
                     /locus_tag="IWO38_001891"
     CDS             complement(11522..12229)
                     /locus_tag="IWO38_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ0313977.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(12308..12646)
                     /locus_tag="IWO38_001892"
     CDS             complement(12308..12646)
                     /locus_tag="IWO38_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ0313978.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(12757..13719)
                     /locus_tag="IWO38_001893"
     CDS             complement(12757..13719)
                     /locus_tag="IWO38_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ0313979.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGL
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(13734..15065)
                     /locus_tag="IWO38_001894"
     CDS             complement(13734..15065)
                     /locus_tag="IWO38_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0313980.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(15073..15753)
                     /locus_tag="IWO38_001895"
     CDS             complement(15073..15753)
                     /locus_tag="IWO38_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ0313981.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(15972..16451)
                     /locus_tag="IWO38_001896"
     CDS             complement(15972..16451)
                     /locus_tag="IWO38_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ0313982.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(16578..18257)
                     /locus_tag="IWO38_001897"
     CDS             complement(16578..18257)
                     /locus_tag="IWO38_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ0313983.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            18805..20307
                     /locus_tag="IWO38_001898"
     CDS             18805..20307
                     /locus_tag="IWO38_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0313984.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            20320..20982
                     /locus_tag="IWO38_001899"
     CDS             20320..20982
                     /locus_tag="IWO38_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0313985.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(21083..21286)
                     /locus_tag="IWO38_001900"
     CDS             complement(21083..21286)
                     /locus_tag="IWO38_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ0313986.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
BASE COUNT         6354 a         4435 c         3635 g         6969 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcactatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca tctacccata
     9661 aataaataac ataaataatt ctaaaaatat tattgataat tatcaaaaca tatttagtta
     9721 tatccattaa aaatcaattt ttccgcctta aaaacaagaa tatcttgaca aaatacccta
     9781 ggaaataaac aatagaataa acaaggtaac aaaaaaatta aataacgtta tataaaaaag
     9841 aaggcgacac tctgtgagaa taacacagac tgaaatgact ttccactcac agtttttgtc
     9901 taactatata caaaaacaga ataaaaagga ggtatttatg ctaattgaag caagaaaaaa
     9961 agagttactt gttctacaaa tggtcattca aaaaaataat tgttctttac aagaattatc
    10021 caatgactta catattccaa aacggacaac caaagaactg atccgaaaaa taaatgtatc
    10081 actcaagcaa tttttattga ttgatgaatt tatttattct aatcataaag gagagatcca
    10141 aaccaaagaa accgatcaaa ctaaaaagtt agctgttttt tctaagttaa aattatgcta
    10201 cctaaaagaa tccaatcgtt ttaactattt gatgttacta atcaattatc cacaaactgc
    10261 tgtaccaaaa aaatatttac ttgaacaact ttatatatcg ccctcctatc tcgaaaaact
    10321 gacaaaacag ctcaatcatt ccttaaaaaa attccattta cggatcctat ctgtaaaaaa
    10381 ttgctgtact cttgaaggaa atgagttatc tatccgtctc tatctttact tttttctttc
    10441 agatacattt ggaggtaacg attggccatt ttcctcactt gaattaaatg cactaaaaat
    10501 gaacagtaca gatgagcaaa tagaaaaaat ttcaaaacaa gaaaaaaata tctacttgct
    10561 gttaacttta ttcatattac gaaacaaaca acaggcatat tttccaaaaa cttcttctga
    10621 aatcactgaa ttgatggact tattgcagca tacacatgat tattcagaat actttacttc
    10681 tttttcagtg agtcgccctt gtgaagagac agaaacagag agagcctact ttaattattt
    10741 tttctattgt ttttttccaa atcaaatctc ttttcgacaa aaacaggaga ttggtttttt
    10801 attttccaca agcaaaaacc ttatttgtcg acaagtcggt caattgatga cttacttaac
    10861 tcaacagttt cctctcaacc atttagaaaa taaaatacca atttattttt attttcttgt
    10921 gttggcagtt gtttttttcg aactgttggg tgaatccagc agtagttttt ctacactttg
    10981 ctttccagaa gccacgtcca atcattctag ttcttttata caggaaaaga aattaagaga
    11041 aaaaatcgat acaatttttt caaaaaatgg ggaaagaaac caaatcagtc aataccttag
    11101 tcaattgatc caacacttac tacaaacaga aacgaaagag cagttactca tccatttgca
    11161 gataaccaaa cagtttacag gaaattatca tcttcaaagc catttgcagc agttatttcg
    11221 caaagatgct gttaaaatta ccgcatattc agaacatgct gatttgatca ttacagatca
    11281 gcatgagaaa atttcagatg aaacggagac tttctatctg gatacaacgc acaatacagt
    11341 gttatttggc aagttaataa aagcggttca taaactgtat atgaaaaaag gttatgagaa
    11401 caaaacaaag aggatgtgac aaaattcttg tcacatcctc ttattccaaa taaatggtat
    11461 tcaatcagct aactctcggt attagctcag atcaaagcgc tgatttcaca acttctttat
    11521 tttaatctct tcggttgccg ccgaacaata agatcaagcg caacagctgc aggaatgtcg
    11581 aaagcgctgc tgcaacataa gtcagtgctg ctgcaaataa tacgtgtctt gccatcggta
    11641 cttcttcttc cgtcagtagt ccgccttcac tcaaaatttg gattgctcgc cgagacgcgt
    11701 taaattcgac tggtaatgtc accaattgga aaagtaatgc taaagaaaaa gcgaaaatcc
    11761 cgatattgat taacgtagag ttccagctaa gcaatacacc taccataatc aaggggaacg
    11821 aaatcatcga accaaagtta gctgctggaa caagtgctgc acgaatcttc aatgggacat
    11881 aacccgaagc atcttgtacc gcatgtccgc attcatgagc tgctacacct atcgcagcaa
    11941 ctgaagtaga ctgtgcagtt gcttgagaca aactcaacat cttattacct gaattgtagt
    12001 tatctgtgag atctccggca atttgttgaa caccgacatt atttatctgt tgactttgta
    12061 atatatattg tgctgcttgt gtaccagtca cattgttttt gcttctcact tgatcgtatt
    12121 ttcggaaagt actattgacg tacgctgacg caattccgga aataactaat ccaagaatga
    12181 ccaaaagaaa tgtcggatct aaaaaaccat acattccata accatacatt ttcgtttcac
    12241 tccttttcgt ttactttatt ttatcattag tagtaggaaa agaatgtgaa aagaccttga
    12301 atattcttta attctttttc acaaattccg atttcagttt catcggtcca aatccttcta
    12361 ccttgcaatc gatattatga tcgccttcta ctaaacggat gttcttgact tttgttcctt
    12421 gtttaatggc acctgacgca ccttttactt tcaagtcttt aatcactgtc acgctatcgc
    12481 cctctaacag acggtttcca tttgcatcta ataccactaa tccgtcttct atgacagttt
    12541 cttgttcttc tttgttccat tcgttcccgc attctgggca aatcaacaag cctcgatctt
    12601 catatgtgta ctctgagcca cattctgggc aatttggtaa tgtcatcttc tttctccttt
    12661 aaaatcttta gtgagcacta ttgtaacgga aaaagaagat cttgaaaaga tacatttgtg
    12721 atttttacta caaaaggcac gttttcgaac gaagtatcat tctgtttttc tgctcttata
    12781 cgattggcat atatcttgga tctctttata gatttcctct acttctgcta aatcttttgc
    12841 attcgcacca cttgctaatg gatggcctcc tccatgatgg cgtttagcca attcattgat
    12901 gatcggcccc ttagaacgta aacgtacgcg gtaataccct gaaggctgct gaacaaaaat
    12961 cccccaagcc agcacttcgt caatcactcc aggaagtgaa acgatcgaag ctgtctctga
    13021 atcatcgatt ccatatttat caagaatttc ttgcggaagg attattcttc ctgcaccatt
    13081 ttcatccacc tggatatttt gataaagata accggaaagt ttagcgactt tcatcggcat
    13141 ttgttcaatc tctctgttca aagcacttgc atcaaatgga taatttctca atatcgaagc
    13201 aatctctaat gtacgggacg tcgtggaggg atacaaaaag agccctgtat ctccgacgat
    13261 tcctgcataa agtaaccgtg cagccttaac gttcatcttt aaagattcag gaaacatcaa
    13321 agcaaattct gcaatcatct cactacagct acttgctttt gtatttaccc acacaagatc
    13381 tccataaggg tcatcattgg gatgatggtc gattttgatc agtttttctg ccaactgata
    13441 tctttgatca ctgattctgg gtgaattagc tgtatcagtc acgatgacta gcgctccttg
    13501 gtagaagaca tcttcaactt cttgcatttc tgctaaaaag cgaagccctt caatcgtttc
    13561 acctacttga cgtatttctt tttctgggaa actttcccgt aatagttcgg ctaatcctac
    13621 ttgtgatcct aatgcatcag gatcagggcg caaatgcctg tggataatga ttttatcgta
    13681 tttttttatt gtatctacta tttcttgctg tactgtcatt tcactcctgc tttctaagaa
    13741 cgttccatta cttgacaaac aacgattgct ttggaaacga tggtgttttc aataaacact
    13801 tcgatatcca gtttagcgga acgtctgccg atttccaata cacgtggacg aatgtctaat
    13861 tcgctttcca attggatcag ccgcaaataa tgtaggttca cttgttcaat caagatattt
    13921 cgtttttggt tcatcaccat tgttttttgt gcgacatcag aaatgatttc gctaagtacg
    13981 ccaaaagaaa tggttccaac gccattgacc atttgtggtg ctactacgaa tttgaactta
    14041 gggttttcca aacgatttcc ttcacggtca gtatcaatcg tatggatatt tccagatatc
    14101 tgatccgaaa tcgtatctgc aatctgcggt tgtcgctgga caagctgcat cgctttcatt
    14161 acatcttgcc gggtaatcaa gccttccaat gtcaagtcat cggaaacgac cggcatgact
    14221 tctagcccat cccagatcat ttgatggcta acagaggcaa tactcattgt tttctttaca
    14281 ctgattggtt ctttcgtcat cacccgatcg atgatctggt ttggcgattt ccctaaaaca
    14341 tcttttgccg taacaatccc catcaaacga tgatggcggt tgacgactgg aaaacgcgaa
    14401 tgctgcgttt cttctgacaa acgctgataa tcagaaatcg tatctgtctg ctgcaaataa
    14461 tgagtctttt cagctggcat atagatatca ctgacaagca tgatatcttt tttgatcaat
    14521 tggtcgctta acgctcggtt gatcatggtt gcgactgtaa atgtatcata agaggtgcgt
    14581 aagatcggca tttcaagatc atcagctagc tttgcaatct cagcagtcgt attgaatcct
    14641 cctgtgatca atactgcggc gccatcttcc aatgctaatt tttggacttc cgcccggttg
    14701 ccgacgatca ttagtgatcc tggcgtaata tagcgttcca tggcatgttt agtcatcgcg
    14761 ccgatgacga atttattcaa gaccttgtct aatcctgccg ccccgcctaa aacgtcccct
    14821 tcaatgatcc tgacgacctc gccaaatgtc aaacgttcaa tatgtttttt taatttgcgt
    14881 tcgatacgga tggtccctac tcgttgaatt gttgaaacta gtccgacatt ttctgcatct
    14941 ttgattgcac ggtacgctgt tccttcactg acattcaatt cttttgcgat gcttcttaca
    15001 gaaattcgat tgccaacagg taaactttca atatattcta aaatcaaatc atgttttgtc
    15061 gccattttct gcctacactt ccagcgtttc cgagacgctc attatttttc cttcactatc
    15121 ttccagtaac tctacaaaag attcgggatc ttgttcaatg acagggaaag tattgtaatg
    15181 gattggaact actttcttgg cacgaattgt ttgcgcagcc tttgctgcat cttctggccc
    15241 catcgtatag ttatcgccaa tcggcaagaa agcgacatca atatcgaatt cttctgcaag
    15301 caatcctaga tcactgaaat aagcagtatc ccctgcatga taaatcgttt ttccttctgc
    15361 ttgaataata attccgctag gctcgcccat atactgaacg ataccgtcta cttcatagct
    15421 tgaactatgc tgtgcatgta cgaattttac cacgccgaat ggaaacgcat gttttccacc
    15481 gatattcata ccgaatgaat ctactcctct gctgttcgca taatcagcaa tctcaacaat
    15541 actgataatt gtggcttgat tttcttttgc aataggaatc atatctccta tgtggtcact
    15601 atgaccatga gtaatcagca accaatctgt ctttacttct tctgcagtta agtctgtcaa
    15661 tggatttcct gtaataaacg ggtcaatcat cacgcgttgg ccattttcca tttcaattgt
    15721 taaacaggaa tgtccatgat acgtaatttt catattttac acctcgcgtt ttctctgtat
    15781 cttttttatt aaaaacgatt tcatcacttg cacatcaatg aaaaaatata gctgctaaat
    15841 atattttcat tggattgata gtacagtatg tacaaagcaa ttgtaacaga taatacagga
    15901 tgcaccaagt accaagcaga tataatctca agtttgaggt ctctgctctg cttagcttct
    15961 cttctcgtcc cttaactaat taaatcagcg taaataggat ggacgatctt ttctaaatcg
    16021 gctactttta tccgaaggct tctcccgatt tcaccgcttg acacaattac ttcttctttt
    16081 tcttttaaag aactgtctgc taaaaaacaa taatcaggat gtgcgagttt tattccaata
    16141 ggggtgtttg ctccgtgggg atatcctgta tgcattaata caaaatccat tccaggaaaa
    16201 ccaactttac gatctttcgt cacttgtctc gtttttttgt aatctaagtg atcgttcaaa
    16261 ggaatcaatg cgatgatcac cccagttttg tttcctttca ctacgagagt tttacatatc
    16321 aaagctggat caatccctct tttttgtagt tcttctgtga aagattcttc taccacatga
    16381 ctaaaatcaa gtggttgata gctgatcttt tgttcttcta aataacgttc gacttttgtt
    16441 tgactcatca ttgattcact ccctttattc tatcgtttat tataaagcga gggatgctgt
    16501 attcaaaaat tatttgtttt tcgcaaaaaa agagcaagac gagcactcta tagagaggtt
    16561 catcttactc tttttgttta ttttttattt cgacgttgag tagcttccat aatgactggc
    16621 aagatcactg ggcggcgttt tgtttgttca aataagtaac ggcctaattg atctcggatg
    16681 tcttgcttta atttgctcca ctcaaaatca ttgctttcta aatgtttctc aacgattgtt
    16741 tcaatcatat tgctgctttc tttcatcaga tcacggctag cttttacgta aacgaatccc
    16801 cgtgaagtaa tttgaggttt tgaaatgatt ttcttttctc gccgactgat agtgacaacc
    16861 gctacgaaaa tcccgtcttc tgacaagatt tttcgatcac gtaaaacaat gtttccgata
    16921 tcacctatgc ctaatccatc aatcattaca ttttctgcac ttgtagttcc tgcaacactc
    16981 atacgcttgt tcttatattc cagcacgtct ccgcgtccag taatatagat atttttgtat
    17041 ggcatcccta ctgcatgagc caaatctgca tgagcagcta attcacgata ctctccttga
    17101 actggcacaa aatatgttgg tttcatcaaa ttgatcatta attgcaagtc attaggattg
    17161 gcatggccag aaacgcgcat attctcagag atcaacttca ctgttccacc agctcgataa
    17221 atgatatctt ccgttttcgc cacgatggtt tccatcgcga tgcttggcgt agtagtgata
    17281 taaaccaaat ctccttcttc aatcttgaca ttacggtgtg taccgtttgc cattttttgc
    17341 agagacttga tgggttcacc cattcggcca gtttctaaaa tgatcagttc ttctggatca
    17401 tagtttttca tgtctttcaa cgtaacaagc agatcttcac ttggtaattg aagtttttct
    17461 aaacgcatcg ctgtgcgaat aattcgttcg aaatcttgac cagtcaagac aacttttcgt
    17521 tgtgcttttt ctgctgcatc caatacttgt tgcactcgtt gcaaattact tgctacacaa
    17581 gcgacgatga tacgcccttc ccaatatttg atcgtatcaa atacttcatc tgctatttgg
    17641 gcttcagaag ctacaggctg aggattttct gcatttgccg attcacttag taaggctaag
    17701 actccttctt tgccgatttc cgccaatcgt gcataatctg tttgatacat tgggatagcc
    17761 gtttggtcaa atttaaaatc tcccgtataa acgatactac cttcttttgt tttcagattg
    17821 atccccactg aatctggtat cgtatgagtg gtacggaaaa agctcactac tgtttcacca
    17881 aagtctattt ctgtatgctc atctaccaca tggaaatcct taaatccttt ggctgcttca
    17941 ttacgactga cattcaattt tgctaactcg atcgtcaact ctgtcccgaa tactggtaca
    18001 gaaatttttg acaacaagta aggaagtgcc ccgatcgcat ctgcatgtcc atgtgttaaa
    18061 aagacaccag ctacacgatc aatgttttct tctaaatacg taaaatcagg gattacgacg
    18121 tcgatcccca acaactcatt ttcaggatat tttagcccac aatctaaaat aaaaatatcc
    18181 tcgcccactt ctgcgacata catgtttttt ccattttcac gaacgccgcc taatgggata
    18241 attttaatgg tactcacaaa cttcacctct tcttatgaca tccaagttta aagatgtcga
    18301 ttttcttctt tatctaaata tatgagccat tccaatgaag catatgcaaa atcactattt
    18361 ctcaacttat ggagaatcag caggaaaatt accagcacat ccgcgttctg acagttggat
    18421 gggcatttta ttttggatat acttcattaa caaaatggat cttttgcgaa acgctatagc
    18481 cagaatactt atataagtat tcagctgttc cccattatta tacaacaaat aaactaaatc
    18541 gctagtatca gcaattgtta gaacaactat gcacgtaaaa atccctctgt agcatctagg
    18601 atttcttttg ctattcctct ttcatttctt cttcgtcggt ttgaaacata cttttttcat
    18661 tttaccacat tttttgcaat aactatagtg actttttata tatacagaac ctcatgttga
    18721 tggtgtgcaa acagcataag atttaagcta gctttaagat aggttgtcta ttatcaaatg
    18781 catctactag gagggatgca aaatatgaat tttatgaaac gtgcatggaa aagtacaaaa
    18841 gtaaaatggg gacgttcatt gttattgttt gccgtgttca cagccattct tatttttgtg
    18901 ctagctggcc tcacgattcg aagcgctgca gaagaagcag cgaatcaagc acaaaaagaa
    18961 gttggagcaa cagtgacttt aagtgccaat cgagaagctt catttaaaaa acaggaacaa
    19021 acttcttcaa gcggttctac cactgaagaa cgtccagatc caggcagttt ttctcttaca
    19081 ccagtttcct taacggacgc ggaaaaaata gctgaattag ataatgtaaa gagttattcc
    19141 tttgaaagtt ccgcctcagc tatggcaaaa gatggaataa ctgccatcac tagttctgat
    19201 tcaagtgaag acagttcaga aactactgat gcaggcggtg aacctgctgg aggaatgggc
    19261 gggaatccgc aaatgactca agcagacttt caaatcagtg gtgtgtcagc aactgcacaa
    19321 aatagtaatt tttctgatgg aacagcaaaa atcacggatg gtgaaggaat agatgacagc
    19381 gacaaaggca caaacaatgt agtcattgat tctactctag cagaaacaaa tgacctggca
    19441 gtcggagata ctttcacaat cacgagtact gaagatgaag ataccactta tgaaatgacg
    19501 atcaaaggaa tttatgaatc cagcgaaaca agcggcagta tgggtatgaa attcaacttc
    19561 atgaatccgt cgaatacact ttacacttct tacacttttg caaatgaact aaatgggaca
    19621 tctgacgata acacgataga ttctgctatt tacactctat ctgatccaga taaaatggat
    19681 gagtttctca aagaagctga gaagctgatc gacacagata catttagctt acaatcaaat
    19741 gattccatgt atcagtcaat gttggaacct ttaaacaacg tggcaagttt ttctaaaaat
    19801 gttgttttac tagtggctgt agccggtatt atcatactta ctctgatcat tatgattacc
    19861 attcgggagc gtcggcatga gttaggcgtc ttgctatctt taggagaatc cagaagtaaa
    19921 atcattctcc aattgtttac cgaagtggct atttgtatga ttcttgcatt aggagttgct
    19981 tcgcttagcg gaaacgtcgt cgcgaatgcc gtgggacagc agctacttga ccaacagaca
    20041 gaaaccacga cacaaaacca gcaagctcct ggcggtggac aaatgccagg taacagacaa
    20101 gaacaaggcg gtcaacgcga tggtcaacaa ggaaccacga ataatccatt tgctgtttct
    20161 gatcaagtca gcgaattgga aatcaatgtc aaacctgcac aattgggact attagccgga
    20221 gtagcatttg gaatcagtct gttttctgtc tttttagctt ctatagggat attaagatta
    20281 aacccaagaa aaatcctatt aaactaaagg aggaatttga tgttacggac agaacaattg
    20341 ggattttggt atcaagatcc gcaagatgcg ctttataaag atgtagaact gaatttcgaa
    20401 gctggaaaaa tgtacgctat tttaggagca agtggttctg gaaaaacgac ttttctttcg
    20461 cttatttctg gactagatgc accaaaagaa ggaaaggtat actatcaaga tcgttcactg
    20521 aacaaaatcg ggttaagaag ttatcgtaaa aatgatgtct cgattatttt ccaagcttac
    20581 aacttgcttc cttatttatc ggctctagac aacgtcatag cagcaatgga aatcactaag
    20641 accaagcaag agaattatcg acaagttgct ttagatcgac tggctcgtgt aggaattgat
    20701 gaaatattag caaaaaaaca ggtgaccaaa ctttcgggag gtcagcagca gcgagtcgct
    20761 atcgttcgtg ctatctgctg tgaacatcaa ctaatcgtag cggatgagcc tactggaaat
    20821 ctagatgaaa caacttcaca agatatcgtc aaacttttcc aagaaatcgc ccatgaacaa
    20881 aatcgctgta ttatcatcgt gactcatgag attgaagtag caaaaagctg tgatgaagtc
    20941 tttgaactaa aaaataaaaa atttcaaaaa atcgaagttt aaaaaacagg ataatgatca
    21001 aataaaaaat ccgaaccata tctgtctcgc agatatggtt cggatttttt atttgcttga
    21061 ttcaattagt aataagtggt ttttattttt tttcgtcttt tcgagaaaac ttgtctttga
    21121 cttcgtcgcc taaatttcga gctttttctt tgatgtcagc acctagtgat tgtgctttcc
    21181 cttctgtttc tttgctcttg tcgtctgttg ctttgccgta tgcttctttt ccttcaccga
    21241 ctactttatc tttcatatct ttagaacgtc cttttaaatc tgccattgtg cttcctcctc
    21301 ttagtttgtt tattgatcta ccttcagggt aacatcaaca acagatacag acaaacagaa
    21361 cgctcgtttt ctaaaaaaga tagttagagc tgt
//
LOCUS       DACECY010000047        20789 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.051, whole genome shotgun sequence.
ACCESSION   DACECY010000047 DACECY010000000
VERSION     DACECY010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.051"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            179..2107
                     /locus_tag="IWO38_001901"
     CDS             179..2107
                     /locus_tag="IWO38_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ0313987.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            2245..2700
                     /locus_tag="IWO38_001902"
     CDS             2245..2700
                     /locus_tag="IWO38_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ0313988.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            3170..3439
                     /locus_tag="IWO38_001903"
     CDS             3170..3439
                     /locus_tag="IWO38_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0313989.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            3839..4093
                     /locus_tag="IWO38_001904"
     CDS             3839..4093
                     /locus_tag="IWO38_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ0313990.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(4182..5228)
                     /gene="holA"
                     /locus_tag="IWO38_001905"
     CDS             complement(4182..5228)
                     /gene="holA"
                     /locus_tag="IWO38_001905"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ0313991.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(5266..7539)
                     /locus_tag="IWO38_001906"
     CDS             complement(5266..7539)
                     /locus_tag="IWO38_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ0313992.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(7532..8071)
                     /locus_tag="IWO38_001907"
     CDS             complement(7532..8071)
                     /locus_tag="IWO38_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ0313993.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(8207..8425)
                     /locus_tag="IWO38_001908"
     CDS             complement(8207..8425)
                     /locus_tag="IWO38_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ0313994.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(8422..8667)
                     /locus_tag="IWO38_001909"
     CDS             complement(8422..8667)
                     /locus_tag="IWO38_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0313995.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(8783..9511)
                     /locus_tag="IWO38_001910"
     CDS             complement(8783..9511)
                     /locus_tag="IWO38_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ0313996.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            complement(9670..10713)
                     /locus_tag="IWO38_001911"
     CDS             complement(9670..10713)
                     /locus_tag="IWO38_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ0313997.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            complement(10703..11194)
                     /gene="coaD"
                     /locus_tag="IWO38_001912"
     CDS             complement(10703..11194)
                     /gene="coaD"
                     /locus_tag="IWO38_001912"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ0313998.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            complement(11191..11751)
                     /gene="rsmD"
                     /locus_tag="IWO38_001913"
     CDS             complement(11191..11751)
                     /gene="rsmD"
                     /locus_tag="IWO38_001913"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ0313999.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            complement(11870..12208)
                     /locus_tag="IWO38_001914"
     CDS             complement(11870..12208)
                     /locus_tag="IWO38_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ0314000.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            complement(12192..12629)
                     /locus_tag="IWO38_001915"
     CDS             complement(12192..12629)
                     /locus_tag="IWO38_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ0314001.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            complement(12619..13743)
                     /locus_tag="IWO38_001916"
     CDS             complement(12619..13743)
                     /locus_tag="IWO38_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314002.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            complement(13764..17192)
                     /locus_tag="IWO38_001917"
     CDS             complement(13764..17192)
                     /locus_tag="IWO38_001917"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ0314003.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            complement(17218..18381)
                     /locus_tag="IWO38_001918"
     CDS             complement(17218..18381)
                     /locus_tag="IWO38_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0314004.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            complement(18384..18686)
                     /locus_tag="IWO38_001919"
     CDS             complement(18384..18686)
                     /locus_tag="IWO38_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ0314005.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            complement(18800..20572)
                     /gene="recQ"
                     /locus_tag="IWO38_001920"
     CDS             complement(18800..20572)
                     /gene="recQ"
                     /locus_tag="IWO38_001920"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ0314006.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
BASE COUNT         6027 a         4310 c         3578 g         6874 t
ORIGIN      
        1 tcttatttga gaaatgttct ggttttttat tttctttaat aataatcatt ataaataata
       61 aaaacaacat caagcttaat caccaaatga taaccattat cgttctcatt tagctgttat
      121 tattatcaat tacattttta ttatgctatc cttgttttga caaggaggtc taataacgat
      181 gactaatcag aaaaaagctt ttgcagcaac aatcggctgc cttttattta tgatcttcgg
      241 ttttatcttc gaccgattct ctcttaccat caccccactt ttttatatca gtgccattat
      301 catcggtgga tataaacaaa catcggaagg cttgaaagaa ttgtggaatg atcggacact
      361 gaatgtggat ttattgatgg ctttagctgc tattggtgcc tgtttgatcg gtaattactt
      421 tgaaggagca atgttgacat tcatcttttg tttaagcggt gcattagagg aatacacaac
      481 taataaaagt caaaaagaaa ttactgcttt gatgaatctt caaccacaaa aagcccaatt
      541 attaaaagaa aatggacaaa tgcaagaagt agatgtttct caactaaagg tggataactt
      601 ggtatttgtc gctaaaggag cagctgttcc gatagatggc tttttagaaa gcacacaggc
      661 aacgatcgat gagtctgcat taagcggtga atctgtgcct gtagaaaaat atttaggcga
      721 tgagatgtat gcaggaacgc tgaatcaagg aaatcccatc acgattcgag taacaaaaac
      781 gagttccgaa actgtctttg ctaagattat ccaattagta gaagaagcgc agaatacgcc
      841 aactcaaaca gcttcattta tcgaacgaat cgaaaataat tatgtaaaat tgattttgct
      901 tgctgttcct ctaatgatcc ttctccccca ctttttccta ggttggagtt gggatgaaag
      961 cttttatcga ggaatggtat tattagttgt ggcttctcct tgtgcgcttg tcgcatctgc
     1021 tacaccagct tctctagctg ctctttcaaa ttctgcaaaa aatgggattt taatcaaagg
     1081 cggtatccat ttagaaaagc tttctgaatt aaaagcgatt gcctttgata aaacaggaac
     1141 gctcactaaa ggaaaaccag ttgtaactga cagctttttc tttgatgaaa aggatttggc
     1201 acaacaactt cttgttgcta tggaacaaaa aacgacgcat ccacttgctc atgcgatttt
     1261 gcaatatttc gattgcacaa tacctgaaga aatccaatac ttaccaatag aagaaattac
     1321 tggttttgga ttacaaacta cctatttagg tgcgcaatgg aaagttggca agcacgcata
     1381 tgatcctgag acgatgataa tcagcaaaga gattgcggaa atgatcgaac gtttggaaaa
     1441 tcaaggcaaa accgtgattt atctttctaa agatcagcag ttaattgctg ttttaggtct
     1501 gctagatatt ccaaaagcaa atacccaaca agtaatttcc tactttaaat cccaaaacat
     1561 ccatacaagt atgataaccg gcgaccattc tggcacagca aaggccattg ctgaacaagt
     1621 agggattgaa cattactatg caagctgtac tccggaagaa aaaacacaac tagtcaaaaa
     1681 agagaatgaa ctttaccaag taaatgcgat ggttggtgat ggagtcaatg atgcgcctgc
     1741 tttagcagcc gcttctttag gtgtcgctat ggggcaaggg acagatgccg ctatggatat
     1801 cgcagacatc gtcttaatga aaagcgactt aaataaattt gtctacagtc atcggctatc
     1861 tttaaaaatg cggcggatca tcaaacaaaa tattattttt tcgattcttg ttattctctt
     1921 gttgattgct tctaacttcc tgcaattttt aaatctacct cttggagttg taggacacga
     1981 aggaagcaca atattggtta ttctgaatgg cttgcgttta ctaagacctt tacctgatct
     2041 ttctgattct tcaaaaaaat gtaagtcttg tccactatac aaagcgcggc tgtcttctgt
     2101 caaataagca aagtgttttt ttatgatttt gtttatttta tgaaaaaaac agcttatgaa
     2161 taggtcttga aggatgaatt cctacttttg taactttcta aaaacatgtt atgattgttt
     2221 cgaagaaagt gaggaatata tttcatggaa agttggcttt ttttaggaat tattttactt
     2281 gtagcaattt tagctaaaaa tcaaagtctg atgatagcgg cagcttttgt tttgattatg
     2341 aagctcatac cagcctcccg ttcctttttc ccttggcttc aaggcaaagg aatcaatatt
     2401 ggggtgacga tcatcacagc ggctattcta atccctatcg caacaggaga aatcggtcta
     2461 cgtgatctat tagaagcctt taaatcccct attggctggg tagctgtttt ttgcggtggg
     2521 ctagtggctg tcttatcttc caagggtgta ggattgattg cttctgatcc acaaatcact
     2581 gttgcacttg ttttcggcac gattttaggt gttgtcttct taagaggaat cgccgccgga
     2641 ccggtcattg cttctggtat cacttactgt atcttacaag tatttgcttt aggaaaatag
     2701 aacaaatttt gtctatttta ggagattctt aacaccccca atttactgat ccgtgatata
     2761 ctgacaacga ggtgatacaa tgttcgaaaa attaaagtat ttcataggaa caatcggctg
     2821 gtttcttcta gcggtacttt tgattctggg cttagcttat ctattatttc ctgatttgat
     2881 acaaaatgtg tttgatactg ctttttgatt gacctattaa aacgatatag gggatgctcc
     2941 tgcgatgcag gggctttttt taatcaatgt tttgtaataa tttaggccgt gccaatgaga
     3001 cttcctatag aattcgatgc tcttttttct ttttggctct tctgctcttt tttgttatac
     3061 tagattacag tggacaaaag aaagaatgat cgcatccact tcaacgaata tcaaaatgga
     3121 agaattagtg cctgttttca ggttactttg aaaagaaagg aagacgcttg tggaaacttt
     3181 tgaaggtctt ttaattgata cgtacgtttt acaagaacag ccaatgatgc tgactgggat
     3241 caacttgcag cttgaggacc gtgtgatcaa tggcctgctt aaagatcgct atttggcaaa
     3301 atatatctta cgtttgcccc taaataaata tgtgctggaa gtaagtggca aatggaaccg
     3361 tcggaaccag ttggtcataa aaaaaatgtt gataaaaaat atcgatgaat acattaaaat
     3421 aatcggtaca ccagaatagc ctttactgat caaaggatac tggttataaa ataactgcag
     3481 tttcttaata gaaaaacaaa gaacaacagg gaatcgtatc ccttttcaaa aaaaactttg
     3541 caaacggttc tctttgttat ataataactt agtaccaaac attcccattc aaactttgtt
     3601 taaatagata aataaaaatc tagtttggtt tgacgctatt gcctcccaca atagcgtctt
     3661 tttgattttt ttcagcttgt actatccgtt tttactgaaa aaaaatccta ataaaagact
     3721 gttaaggaaa aaaacttgtc atgttcatat tgcaaagcac gctgccttta ggtataataa
     3781 tgaatgttga attatgcggt aacacgcgta agaacttaag ttatcggagg tgaaactaat
     3841 gccaaacatt gaatctgcaa tcaaacgtgt tcgtacaagc gaaaacgcta atgtgaaaaa
     3901 ttcatctcaa acaagcgcta tgcgtactgc aatcaaaaaa tttgaagatg ctgtagcttc
     3961 aggtgctgac aacgtagatg cgttatataa agaagctgtt aaagctattg atatggctga
     4021 atcaaaaggt ctaatccaca aaaacaaagc taaccgcgac aaatctcgtt taagcaaaaa
     4081 aatcgctaaa taaaagaatc ggcttcggcc gattttttta tttgtcaaaa aaaggacaaa
     4141 aacaaacgac cttcccgcat gctttgccct cgtctttctt cttatcgatt tcttaacaac
     4201 tgccctgtgc gcaaaacgaa tagttgaaaa cttaattctt tttcgatttg tcccgttttg
     4261 atttgataat ccatctcaac taattgatca aacaactgta ccagcaaatg ttcatcgaat
     4321 ttttttactt cctgcatggc aagttttaca cgatatggat ggattttcaa tgtttcagca
     4381 atatttgcct gctgataacc aatctttaca agaaattttg tctgtaaaaa taaacgtaat
     4441 tgagaaagca aaatcgcatt aattttgatt gtttcttccc cttgcataac taaatcttcg
     4501 tataaacgca aagcttgttc cgtcttccct tttaaaatat attgggtcat gtcaaaaata
     4561 ttatgttcta acgttttagg aactaattct tctacttctc ttaccgtgat ttgctgttga
     4621 ttttgtccat aaaggaccaa tttctgtaac tctctcatca ttttggataa atctaagtcg
     4681 gtcaacctca agaataaatc aattgcactt cgttccattt tgattgagga attctctaag
     4741 gtattcatca gatattgacg tacttctttt tcattcaact gttggacatt gatgataatt
     4801 gctgtttttt tcaattgttt cgttactttt ttccgttcat ccaacttttc atatggcgca
     4861 aagaatacta atacagtcga ttctaatggt tctttcaaat aagccgtcaa taaatcagta
     4921 ttttgttcga ttccattgtt tactttttca cctgttaaaa aataaggatt ctctacgaat
     4981 actaaccgtt gatctccgaa aaacggcaac gtctctgctt cagctactac ctcatctaaa
     5041 cttgcatttt ccatgtcaaa acttaaaaaa tttaagtcat cttcgccctc taattggatt
     5101 tttttgacaa tttctgtccg aacttgatct tggagaaact tttctgttcc taaaatcaag
     5161 taacaagatg caagtttttc tttacgtatt ttttgtaact ctgcttgtat actcaaatta
     5221 ctcaccactt tcaaaaatta tcctaccatg agcagatgag aattactagt ctatcattgt
     5281 ttccactagc ccatgaggat gaaaaactgg atgccagcga taggtgatca ttccttgttg
     5341 atccgttcgc catgttttta tctgattttc ttctagtgtc tccaatactt cgctgtgcgg
     5401 gtgaccataa cgattattta cgccgcagga tataatccca tgttttgctt caatatggga
     5461 aagaaaatct gtgttacttg acgtacggct accgtgatga cctaatttga ctacatcggc
     5521 tttcagatta ggataaatca aaagcaattg tttttccatt gcttgattca aatctcctaa
     5581 aaataaaaag cgcctattgt tatattccat ccatagacca atcgaatctt catttcttcc
     5641 ctctcccttt tcaaaaggag tcaatacata aagatcataa taaccgctga tcctgtcccc
     5701 atgtttgatt tcaatgatct cagtaccatt tttttgagct tcagataaat gcctttgtaa
     5761 tggctcattt cgtccagcgc cccaaccgat ataaattttt ttgatagtaa aatgctgaga
     5821 taacgtgggt aattctccca catgatccgt atcgtcgtgt gtcaagatga gtttatcgat
     5881 tacacttatc ccctgacctt ttagatatgg aacgatattt ctttcagcag gaggacgtat
     5941 cataccttgc gcccactttt ctctttcaaa gtgtaactga cctccagtat cgatcaaaat
     6001 cgtttctttt cgaaaaggac tttgcaaaaa gatactgtct ccttgaccga catcgataaa
     6061 agcgatcgtt gaagaaaagc gtaaaaatgg caaagtcaaa aaaataaaag aaaaaagacc
     6121 aacataaaac aagtgtttca caggttgttc tttcaaccta tccatacaaa tcagtaatcc
     6181 gaaaatatac atcccagata gcacaagggg caaacgtcct ataatgagag acgaccctga
     6241 agaaaaatcg attattcgct caaataagat taatccctct tctatcagca aaagtaaagt
     6301 ttccggcagc cagcgattga agagtgtgag aaataacaca ccaggaagaa aaagaaactt
     6361 gaataccggt gcaatcagta atgtaaaaag actacctaaa ataggccact cataaaaatg
     6421 ccatacaatc aacggcatcg tgatcatagt aaacaggacg tgagaggccg tttttttcaa
     6481 ccaatctcca tcaaaagaaa tcaggataaa caaaaaactc atgaaaaaac taagctgtcc
     6541 acccgcatga aaaagaaatc gaggatcaaa tagtaaaaga agccaaaaga caactgcaaa
     6601 taagtccatg gtggaataat gcttattcag caatttgact agcaatcgca ataaaaacag
     6661 caaactagca cgcaaaacag aaatagaact tcctgcaata gaaacataac agatagttag
     6721 tagactcaac ggaataatac tgacgtgtag tggccatccg atacgtcgaa ataaataata
     6781 cagccaccct aaataaaatt ggatgtgtag acccgataga ctgaacagat gcagcaaacc
     6841 actctcccta aaaatttctt ctggttctct gaatcctgca tctttatagc cccataataa
     6901 agaattaata tatacacttg ttttttgcgg aaattcagat tggacaaagg agataaaaga
     6961 tcctcttttt ctctgaagcc agtttcgcca gtgattttgc atggtgatct gttcaatctt
     7021 ttgaatttgg aaccgtccag aaattctatg gttcctgaag taagcttctt ggtcaaaatt
     7081 ataaagattc cgcttttttg ctggagagat gaactcgcct tcagcagtca attctattcc
     7141 ctttcctcgg aagttaagcc atgttttcaa ttctgcttct gtgtttactt gataagaaag
     7201 ctggagcttt tgtttattct ctgataacct tccttccatt gtcaaaaaat taccatttgc
     7261 acgaatcgta tctgtaaata cagtaatttg cgcagatgtt gttttttcct ctaaaaatga
     7321 atactcttgt agcctataag aaatggatac cgttatgcta cacaaaatac acaagtacgc
     7381 gataaaacgt ccttctttta gcgaaaacca taaaaaaatt ccggcaagaa gcaaacgata
     7441 accccaattg tcagtgtaat acaacaaaca gataatcatc gacgtaattg ccggaaaaat
     7501 caaccgattt cgaacatgcc tttctaaaac gctagacatg ttttcctgtt aattcatttt
     7561 gaagttttga aaaatgctgt gagtctaaag caacttggtg tacagataca cctaattgtt
     7621 cgatcaacgc ttgggcataa ggatcatttc gatagtcatg gagataattg atccctttca
     7681 caccagcttg caacaaagcc tttgtacagg ccaaacatgg aaaatgtgtg acatagattt
     7741 ctgaaccttc cgtcgggata ccaagttttg cacactgtag gagtgcattc atctctgcat
     7801 ggatcgttcg caagcagtgt ccatccacga cgtaacaccc ttcatctatg cagtggacat
     7861 ctccactgac tgctccatta tagccgccag caatgatcct tttttctcga acgagtgtcg
     7921 ctcctacttc taaacgtgta cacgtactac gtaaagataa cagtactgcc tgagccataa
     7981 agtactggtc ccaaggaatg cgttcattat gtgacttatc agtaactttt tctttattcg
     8041 tatcgcatgc ttttgaagat ttttctaaca tgttttatat tcctccgttt tttcaagttg
     8101 attaaaaagt actcattgct taaacaaaat attctttaaa tataacgaat catcctgtct
     8161 gacaaaagat cctacccttc actgccgaaa tgaagtccgt catccattag cccattataa
     8221 aaattcttcg aatccatttg ttcaagaaat tccgtataaa aatcgatact tcacaatcat
     8281 ggcgttttgc tacttctgtg agaacagaga tatattcttt gatcaacttg ttattccgat
     8341 atttttgttt tgtctcatcg actgctggtg gcgtgataaa gaaagcatga tcagaactat
     8401 agtctttttt cagctgaact atcatccact gcagattttt tggtacgttt ctttagaaag
     8461 ttgtttatgc tctgctagat cattcgtccc aatgagaata aatactttat ctgctgtata
     8521 gctaagtaca tctttggcaa agcgttctcg tagagcttga ctgttattgc cgctgacagc
     8581 cgtattcata atatgaatcc cagatatttt attttggaga tagacattca gcattggttt
     8641 ttttaatgtt tctttgcgaa caatcaagct atttcctatc caaaaatact acctcctcgt
     8701 cctgccaagc tcttagaatt ggattattct tagtatccat tagcgtccat ttattgctcg
     8761 tgccagtcga ttttgaatca ttctatttaa ttgtaacgaa gttttttagt ttttcaactg
     8821 tcttttcacc gataccagaa atctcctgca ggtcttccac tttctggaag gatccgtttt
     8881 cttctctaaa tcgaatgatt tcttgcgctt tgactgatcc gatcccagga atctgctgaa
     8941 gttctgatgg agtggctgta ttgatattta ttttgggagg ctcggaatct gctgtaggct
     9001 gttttccttc cgaggatgcc aactgatcaa gatgttctgg cagctcttca cctacagcag
     9061 ggatatatag gacttgctga tcttctatct tcgcagaaaa gttgattcga tcgctatccg
     9121 cttcttctaa taatccacct gcacgcttta gtacatcata gacccgttct tctgaagaaa
     9181 aggaatagat accaggctcc ttcacacttc ctttgatatc ggtgtacatg acctttggac
     9241 gagcatcctc ttctacatcg tttccttcag ctgatggatc ttctccactg gaactaaacg
     9301 aagaaagtat ttcttttgca gcagtctctt ctgtttctct ataaaatagc aaatttgata
     9361 ataaaaaacc acttgcgata caaaagataa gaactatcat agacatccaa atgatcaatt
     9421 tgggtgaagc tagtattttc ttgctgattt tttctatcat tgttcttttt tcaaagaatg
     9481 accagtaaaa atcattcatt actcctttca tttaaaattg aagaaacagt gagctctggc
     9541 aaaatcgtcc cttcaacatc taattacgtc aattttttct tttgttttta tttttatcgt
     9601 gaagattttt gacgaaaaag aggggttgtg acagaactcg tgtcacaacc cctctttaat
     9661 tatttagttt catttcattt tctctagata gtcaagcgct tcttgtactg tttttactgg
     9721 tacgattttc attgaggaat tcaatttttt agcggcagct tttgcttctt cgtagttggt
     9781 ttttatttca ggatattctt tcttaacatc gtctgaaatc gtatcatctg gtgcaaagaa
     9841 aatctctacg ccagctttat ccgcacttgc cactttttta tcgattcctc cgattcggcc
     9901 aacttctcct tcactgttga tcgttccagt tcctgcaatt tgcttaccat gtcgcaagtt
     9961 tttaccagtg acttgttcat agatttctaa agtgaacatc aatcccgctg atggcccacc
    10021 gatagagcca gaattaatac gaacatctgt atctgattcg atctcggtat ggtcagtaag
    10081 cgtgatacct attcctgcct ttttatctgt tggtaactcg atcagatcac cagaagctgt
    10141 tttttcttca ccattatgca aataactgat ttcgattgtt tgtccaactt tttggctttt
    10201 tacataatcc ataaatgctt ctgtactttg aaagcttttc ccgtctactt ttgttactgt
    10261 atctccaact gaaagcttgc caaagaaatt cgatgttttc tctacactca tgacatatac
    10321 gcctttgaat tccattttat aaggcttgtc tgcaagtttt aacgcttgtt cgattgctgc
    10381 attttgggaa gaatccatat aatactgttg catttgatta tattcttcat ttgtcgcccc
    10441 accagtcaat tcttcttcac tgatcaattc ttcaaaaggg gtcattttag cttttaaagc
    10501 taagaaacct gtagcttgcc tgataccaac agaggtaaga gagaaagacc cagaaccttt
    10561 atcttcctta tcattgaccg taattagttc tttcagatcg atcgtggctc ccggtgattc
    10621 tacataatac ggcaagggaa ctactacaaa tccaatgatc aacattccaa atatgataat
    10681 cagtactgtt ttcaaccatt gtttagcttt catttgattc acgctttcca gctaaagctt
    10741 cgttgatcac tggcggtaag tagctgctca catctccgcc aaagtgcagt acttctttta
    10801 aaatactgga actcacgtga ctatattctg gttttgctaa taagaagaca gtttccaatt
    10861 catcatatag atgatgattc atctgagcaa tctctcgttc atattcaaag tctttgatac
    10921 ttcgtatgcc tctgatcaac gcatctgcac caatctcttt tgctgtttct accgtcaatt
    10981 gattttcttg atgcatgact tttacattag gaagatgaga aacagctttt gtgatcaatg
    11041 tcattctttc ttctggggga aacaaacttt tctttgaagt attgataaaa acaccaatta
    11101 ctacctcatc gaataattta gccgcacgtt ccaccgtatc taagtgacct tttgtaaaag
    11161 gatcaaaact tcctgggaat aatgcacgtt tcattgattt ccctcttttc tatagatagt
    11221 tacggccgtt atgccataaa tctgttttct tatttgatgg aatccagcca cagtttctgg
    11281 caattcgact gttttatccg tttcgcatac gataactgcg gttttagaaa aaagtcccag
    11341 ttcttccatt ttatggattt gatcgattat tttttgtttt gcatatggcg gatcaagaaa
    11401 caagtagtcg aattgttgtt tctccgctgc tagccgttcg attgctttgt ccgcatcaag
    11461 ttttattgtg gtaaatttct ctggttcttt cgtaatttcg atgttttctt tgatgatttt
    11521 tagcgcttgg taatttttgt ctacgcatac cgcatgactg cagcctctag atactgcttc
    11581 gatagctaaa ccaccgcttc ctgcaaacag atccaatact gtctctccat caaaatatgg
    11641 tccaatcata ttgaatatgg attcttttac tttatctgta gttggtctgg tgttcgcccc
    11701 tgctaaactt tttaaacggc gcccgccata atttcctgat attatccgca tctactcact
    11761 ccttagaaat ggtactggta ctagtatacc aaaaaaatac ttggacaatg atcaaaaatc
    11821 gatcgttatc caagttattt tttatttaaa aactgctcat aagagatttt taaagatgtt
    11881 cagctaatcg gatttctgta tttagctctt caatttcgaa accttcgtct tttaatactg
    11941 ttttgtttcc tattttttcg gcaaaattca tttcgatatc cggtcggtaa gaacgttcta
    12001 cgtttcgaac aaaatgtaat ttacggattc gttcttctgt ttcttccaat gtctcttcat
    12061 ttaagtaaag cacgacatat ttcattcttc ttgagacata atgcaacaag ccgaactttt
    12121 tcagattctt taattgtttt aagctgtaga cccatacgat cagcccgcgt cgttttgtta
    12181 tttcaaattc actattgtct aattgcatga caattacctc cgcatccaaa gtgttttttc
    12241 gtttcaaaaa acgggctacc tgcatctact ttgatttcat tagaaacttc ttcggcaagt
    12301 acttggacaa tttgatccaa cagactttgc agctgtgttt cactatatcg gaaagctgca
    12361 acatgctcat ccatatcaag ctgtcttttt gcttttcgaa cagctcgttt tttttcacga
    12421 taatcaggtg catactcacc atatgccgct acattttcaa atgcttcttt cttagacaag
    12481 aacgcttctt tcaactggca aacttttgca gattcttcca ttgactgttt atgttcaaga
    12541 taagaagcca tcgttttact cttttttatc atttcagcga cttggaggca ttgatcctcc
    12601 agttgaaaca gctcttcgtt aacgatcact gctatcacta ctttctccgt tttcttcttg
    12661 caataagact aacatatttt ctttagaaaa agcaatgcct acagaatccg ttcccgggtc
    12721 catgaaacgg tcatggttgt aaggatcgct gtaccaatat aaaaacgaaa aagtagcgtc
    12781 aatcgccgga tgaataaata gcccactagt attttctaat ttttgtttac tgattaactt
    12841 ctccaattca tcattcgtca gtgagaattt cactgtcgtt tgagcactgc ctagcgattt
    12901 ttgccaatca gccatacgtt cctctgacaa tatttcctca ggccttgtca aaaaatcatg
    12961 tagtagcagc tgactgtggt tttgaaaggc aagtgtttga ttgtatatgg gaagttcgtc
    13021 ttctcttcta agcatattta ataaagtcgt actttttcgt tctttaacgc gattgattga
    13081 ttcccaatca gccgatttat ctgcttgata atgtggaagg ccttcaccaa acacttgata
    13141 tggcaatagt ttcaaaagac tatctttatc taaatacaca actgaaaata ggccgtttgt
    13201 tgtttgatca aaaaataaaa tagcaaaagt gtcgttatca aaagcaatta gcggacgata
    13261 attcatatcc tcttccatta gttcgattcc aacttctgta tcatggtact caaaagtgaa
    13321 attagtatag atagtagtga ttttcgtaag atcttgcatc gtcatcccta aataaaacgg
    13381 cgcgatatca gcaggccctg tttgcagtac tttaacagtt gtgactctgc tgtcttggat
    13441 gttcgcttca aatgtgagat gactagcttt attttcataa tatcttgttt caaaaccaaa
    13501 tccgctagtt aatcgatcat aggcttggcc aaaatccttt tccagctgat cgatcggttt
    13561 tccgatataa gtagaaaaac cagttgcttt aagctcctgg tatttccacg ttgtcatttg
    13621 gtttgatatt tgttgatcag agttttcctt cacttcaagt tttggcggaa acaagactgg
    13681 ttccatgtag aatgccacaa taactaatag cagaatcagc caaaacaaac ccagtctttt
    13741 catgtttccc cacctttcgc ttattatttc tcagtcactt caatcaaaag gtcaccagac
    13801 gaaatagtgt ctcctgaaga cacatataca tgtgcgactt ctccgtcaaa acgtgcttcg
    13861 atggccgttt ccattttcat cgcttcagta atcatcaacg tatcaccttt tttaacgtta
    13921 tcgccttttt tcactaatac atccaaaaca gaaccagaca tcgttgctcc aatttgttct
    13981 cggtttgtcg gttcagcttt caatcgggtt tggaccgtac tgatgatcga attatctttg
    14041 atagtgattt cacgtctttg tccattcaaa ttgaagaaca gtacgcgatt cccttcgata
    14101 tcgggttctc cgatttcatc taaacgaatg ataagcgttt ttccgcgttc gatttgtaca
    14161 ttgattgttt cacccaaacg tataccttgg aaaaatgttg gtgtgtccag taatgtaaca
    14221 tcgccaaatt gattatatgc tttttgatag tccaaaaaga cttgcggata catcaaatag
    14281 cttaatactt cgtcttgttt tggttcatag ccaatttttt ctgccaattc tttttttact
    14341 tcgttgaagt cgactgattc agccaaaaga cctggacgtt cattcaatgc aggtctgcct
    14401 ttaagaataa tcttttgcaa ttttttagga aatccgccaa cgggttgccc tagttctcct
    14461 tggaagaaag tcacaacaga ttcagggaaa cttagtgttt cgcctttttc ataaatatct
    14521 tcttctgtaa gatcattttg gaccataaat agtgccatat ctccaactac tttagatgat
    14581 ggagttactt taacgatatc gccaaacatc aagttgacag tatgatacat ttgtttgatt
    14641 tcatcccagc gatgacctaa tccgacagct tttgcctgtt gctgtagatt cgaatactgt
    14701 cctcctggca tttcatgcat atatacttct gtttcaggag cattcaagcc gttttcaaac
    14761 ggtttgtaat acatgcgcac atcttcccaa taatggttta atttctgtgc attttcaatc
    14821 gtgatttctg gcagccgtgg accattgacc agtgcataat acaagctgct catgctaggc
    14881 tgactagtat tcccactcat tgcactcatt gccacgtcaa cgatgtctac tcctgctttt
    14941 gaagcagcag aataagtgat gattccattc ccgctggtat catgagtatg cagatggatt
    15001 ggcagatctg tcgtttcttt caattcacta atcaaacgat aagctgcctg cggtttaagc
    15061 agcccagcca tatctttgat tgcaatgata tgcgcaccca gttgttctag ttcctttgcc
    15121 atgtctttat aatactgaac attgtatttt gcccgacttg gatcgttgat atcgccagta
    15181 tagcaaatag ctgcctctgc gattttacca gtatcccgta cagcttggat acttttttcc
    15241 atttgtggag tccagttcaa actatcaaag attcgaaata catcgattcc ttgcgctgct
    15301 gcttctttga caaattccac caatacattg tcaggataat tagaataccc aacggcattt
    15361 gaacctctga aaagcatttg cagcaacgta ttaggcatta aagaacgaat ttttctcaac
    15421 cgttgccaag gatcttcatt caaaaagcgg taagccacat caaatgttgc tcccccccac
    15481 atctcacttg aaaagagttc aggaagacct tctccagtca aacgtgcaat ctgcttgaaa
    15541 tcctttgtcc gaacacgagt tgctagcaga ctttgatggg cgtctctaaa tgtcgtatcc
    15601 gtcaacaaca aattttcctg tcctttgatc cattcgacca aagcatcggc acccttcgca
    15661 tctaatacat tcttagccgt tacgtaatca gttcttgtga ttagatcttt aggcatacgt
    15721 ggttcttcat aaaatggttt ttcgccactt tcgatacctg gaaaaccatt cacagtaatt
    15781 tcaccgatat atttcatcgt tttgtttccg cggtctcgta gtcgaggaaa ttcaaataat
    15841 gttgctgtac tatcaatgaa ggttgttttt gcatttcctg attggaattc aggatgtgtg
    15901 atgacattga gcataaatgg aatgtttgtt ttcactccac ggattctaaa ttctttcaag
    15961 caacgttcca ttttctgaat ggctgtttca aaggtagcac catgcgtaca tacttttact
    16021 agcaatgaat caaaatacgg tgtcactaca tagcccgcgt aagcatttcc gacatccaga
    16081 cgtacgccaa aaccaccagg tgaacgatac gtatcgattt tccctgtgtc tggtaaaaag
    16141 ttattcaacg ggtcttctgt agtgattcgg cattgaatgg ctgaaccatt taatttaatt
    16201 ccttcttgct gaggcaatcc gatttcttta tgcagatcca aaccttgagc aatcaacagt
    16261 tgcgttgtta cgatatcaat atctgtaatc atttctgtga tcgtatgttc cacttggacg
    16321 cgagggttga cttcgataaa gtaaaaatca tctccttcaa caagaaattc tactgtacct
    16381 gcgttgacat acccgacata tttcatcaat tggacagctg cctggcagat tttttgacgt
    16441 tgctgttcat tcatagagac acaaggcgct acttctacta ctttttggtg acggcgctga
    16501 acagaacaat ctctttcaaa aagatggatg acgtttccat gcgtatcccc gagaatctgt
    16561 acttcgatat gcttaggatt agcgatgtat ttttcaacat acacttcatc actaccaaag
    16621 gctgcttttg cttcgctttt cgcacgttcg tagccctctc ttgcactctt ttcatcgtga
    16681 gctacacgca tgccgcgtcc gccgccgcct agagctgcct tgatcataat cggataaccg
    16741 tattgtttcg caaactccaa ggcatcgtct atagaagcga ttggtccatc tgtgcctgga
    16801 atcgaagcaa tccccgcttc gatagctgca gcttttgctt tgattttatc tccaaaaata
    16861 tctaaatgat gcagtttggg tccaacaaaa atgatgcctt cttcttcgca ccgttgcgca
    16921 aaattcaagt tttccgacaa aagaccgtaa cccggatgaa tagcgtcagc tccgcattct
    16981 ttcgcgatag aaatgatccc ttcaatatct agatacgcat caatcggttt ttttccttgt
    17041 cctactaaat atgcttcatc cgctttgaaa cggtgtacgg aatattcatc ttcttctgca
    17101 tagattccaa cagttttaat tcctaattct gtacaagcac gaaatacacg tacagcgatt
    17161 tcacccctgt tagcaactaa aacttttttc atcgaaccac tcctacatct ttttttatta
    17221 taaacctaag ctcttgcgtt tttcatccgc actgatgttg aggacaaagc cgacacaaat
    17281 cgaaagcatc agcaaactgg agcctccttg acttaaaaat gggaacgtga tccctgtcaa
    17341 aggaataacg ccagtaattc ctccaagatt gacaaatact tggatcaaaa acattgcgcc
    17401 gatcccaatg cacaaaagcg aattgaacgg atcttttgaa cggataccta caaggacaat
    17461 gcgcacaatc ataaagaata gcaacgctaa tatcaaaatc cccataatca ctcccagttc
    17521 ttccactacg attgcataaa taaaatcggt ttgtgcttct tgtaaaaatc cttttttctg
    17581 gatactgtta cctaatcccc tgccaaatag tcctccgttg aacatggcat agtagccgtt
    17641 gactagttga tgccctttat ttaattcgtc tgagaaagga ttttggtaaa tagcaaatcg
    17701 attatagata tactgtaaac gtccgggtaa gatctttccg tttgtgatat tgatcagcca
    17761 gatcgtaaaa gtacttagac aaaagcctcc tactcctaca atcaaagtat acacataatt
    17821 gactccgctg gctaaaagca gaaccaaaat aatcaaaaag ataactgccg cattaccaaa
    17881 atcgggaaga atagcgacta atgcagtaag tccgataacc attgcaagtg gacgtttcac
    17941 agtcccaaga aaatcttttt gtacactgtt ttgtctttta gataaagtca gagacaggta
    18001 ccagatagaa atgattttta ggtattcggc aggttggatc gagaatcctg caatttgcag
    18061 ccaacctttc gccccattga tctccttacc aaaaaacaca acaatcagca gcaaaatcgt
    18121 caaaactgcg atggctgcca tgatcaaacg ctggtttttc aaaacatctg ttttcatttt
    18181 ataaagtagt gcgatagcaa tcaagctaag cacccaaaaa atactctggt taatgactga
    18241 tgcggacgga ttttgtccgt tttctaataa tagatacgac gtagaactat ataccatgat
    18301 caatcccaat acacataaaa ttaaataggg aatcaagatg ctatagtcta atagatgcct
    18361 ttttttcact ttcttaggca agtctattcc tccttgccaa cttctttttt atcataaatt
    18421 tcttggtaaa cctcactata taactgattc aattctcgtt ctagctcact taacatcagc
    18481 tggccctcgt tctttccgat tactcctata cggacagcaa aaccgacttg ccgggaaaag
    18541 ccgtacatct gtgtatctac cacttcttcg aaggctttgc attgtgagat acagagactg
    18601 ttgttttgat ttcggattaa tcgtttgatc cgttctgctt catcatttaa tacttggatc
    18661 gcaagttttt tcgacaaaga atccatctgt gcttacctcc cattctaatt ccttaattat
    18721 aacatatttg attttcataa ggagcaaaaa aaacagattt aaccagttgt ttttattcat
    18781 ttttacgtat tcgttctttc tacttatcca gaacgttaga gtccgcattt tttatccgag
    18841 ataaaaaggc ttcgccatat ttatcaagct tgctttggcc aactcctttt atctgaagca
    18901 tctccaatct gcttgatgga cgtattcggc tcatttcttt caatgtggaa tctgaaaata
    18961 cgacatacgg gggaacgcct gcttctgatg ctagatccgt tcgcagttcc cgcagcactt
    19021 caaatagtgt atcggtttct tcatccgata gctgctggac tttcttgggt tcctttcgat
    19081 aaacagattc ttgtcctttc aatacttgta tccctctttc tgttactttc aaaacaggat
    19141 actcaccatt gatggtcaga agatagccat tcgatatcaa atattcaatt aactgcatcg
    19201 tttctttttg cgattggttt ttcatcagtc cataggtaga taaatgtcca aaacctaaag
    19261 cttgcagctt ttgttctttc gatcctgcta agactttcat cagcatttgt ttaccatagt
    19321 tctcacccat acgttttaag caagaaagaa ccatctgtgc ttctgtcgtt acttctacta
    19381 tttctctgtc atctaaacag ttaccacaac ggttgcacgg actgcttgtc tcgccaaaat
    19441 agtttaggat gtattgctga agacaggact caatatacac gtattctgtc atcgctttta
    19501 atttttcata ttctttttgc ttttgccctt cttctctttg ggactgctgg ataaaaaact
    19561 tttggacttg gatatcttga ggagagaaaa gcaggatggc ttcactggaa agaccatctc
    19621 ttcccgcccg accagcttcc tgatagtatg cttctagaga gccaggaatt tgataatgga
    19681 taacaaaacg gacattgctt ttatttatac ccataccaaa tgcatttgtc gctacaatca
    19741 gctgcaagcg atcgtataaa aaggcttctt gcatttctgt tcgctcattc tcatttagac
    19801 caccatgata tctacccacg gaaaaatcga attttttaag caaatgatag agacggtcta
    19861 cttcttttct cgttgctgca taaatgatac cagattgatt tttgttgatc tttaaatatt
    19921 cgattagata ttgttctttt ttttgatctt taattacttg aaatcgtaga ttttctcgtt
    19981 caaaaccagt ctgaatatgg ttgttttctg gaatttttaa aagtcttttt atgtcagcag
    20041 ctacttgcac agtcgctgta gctgtcaaag caacaattac aggacgattt ggcagttgat
    20101 ctagctggtt cgccatctgt aaataacttg ggcggaaatc atgcccccac tgagagatac
    20161 aatgtgcctc gtcaatagcg atcatacgga cgttccatcg cttcattgct tggataaaat
    20221 ccggcatgat aaaacgttca ggagcgatat aaagtaaacg gtactgctgt ctatcgattt
    20281 cttggaagcg tctagctgtt tcgtaaccat ctaacgagct attgataaaa gtggcgggta
    20341 tacctaattg attcgccgcg tccacctgat ccttcatcaa agagattaaa ggggacacaa
    20401 ccacagtaag cccatctaat agtaaagctg gaagctggta acagattgat ttcccacttc
    20461 cggtaggcat gattcctaat acattttcct gatcaatgac tttctggata atttcttttt
    20521 gaccaggtct aaactcatca tagccaaagt attgttttaa taagtcgttc atcttcccgc
    20581 tcctttctct tgccatttta cttcgatttt cttcgaagga caagttgatc tcctttcgtg
    20641 aaacattatc ttattatgaa acaaaaactc tctagaaata agttttttaa aagtctttta
    20701 aacgtctttc ggcgtatttt ctgaatttat gtccattgac aaagatgact tctccacaga
    20761 aaagagttgg acaaaaaaag aaaccagct
//
LOCUS       DACECY010000048        20179 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.052, whole genome shotgun sequence.
ACCESSION   DACECY010000048 DACECY010000000
VERSION     DACECY010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.052"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(282..479)
                     /locus_tag="IWO38_001921"
     CDS             complement(282..479)
                     /locus_tag="IWO38_001921"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314007.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(625..1503)
                     /locus_tag="IWO38_001922"
     CDS             complement(625..1503)
                     /locus_tag="IWO38_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0314008.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1542..2660)
                     /locus_tag="IWO38_001923"
     CDS             complement(1542..2660)
                     /locus_tag="IWO38_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ0314009.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2657..4756)
                     /locus_tag="IWO38_001924"
     CDS             complement(2657..4756)
                     /locus_tag="IWO38_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ0314010.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4807..5628)
                     /locus_tag="IWO38_001925"
     CDS             complement(4807..5628)
                     /locus_tag="IWO38_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0314011.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5625..6434)
                     /locus_tag="IWO38_001926"
     CDS             complement(5625..6434)
                     /locus_tag="IWO38_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0314012.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6455..6949)
                     /locus_tag="IWO38_001927"
     CDS             complement(6455..6949)
                     /locus_tag="IWO38_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0314013.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6961..7449)
                     /locus_tag="IWO38_001928"
     CDS             complement(6961..7449)
                     /locus_tag="IWO38_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0314014.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(7526..10222)
                     /locus_tag="IWO38_001929"
     CDS             complement(7526..10222)
                     /locus_tag="IWO38_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0314015.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(10650..11723)
                     /locus_tag="IWO38_001930"
     CDS             complement(10650..11723)
                     /locus_tag="IWO38_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0314016.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(11720..12547)
                     /locus_tag="IWO38_001931"
     CDS             complement(11720..12547)
                     /locus_tag="IWO38_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0314017.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(12548..13354)
                     /locus_tag="IWO38_001932"
     CDS             complement(12548..13354)
                     /locus_tag="IWO38_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0314018.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(13366..14451)
                     /locus_tag="IWO38_001933"
     CDS             complement(13366..14451)
                     /locus_tag="IWO38_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0314019.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(14468..15010)
                     /locus_tag="IWO38_001934"
     CDS             complement(14468..15010)
                     /locus_tag="IWO38_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0314020.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            15268..16257
                     /locus_tag="IWO38_001935"
     CDS             15268..16257
                     /locus_tag="IWO38_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ0314021.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            16254..16991
                     /locus_tag="IWO38_001936"
     CDS             16254..16991
                     /locus_tag="IWO38_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ0314022.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNCLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEETQSKSEIAALITEKIRLEESK"
     gene            16988..17539
                     /gene="coaC"
                     /locus_tag="IWO38_001937"
     CDS             16988..17539
                     /gene="coaC"
                     /locus_tag="IWO38_001937"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ0314023.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            17555..18187
                     /locus_tag="IWO38_001938"
     CDS             17555..18187
                     /locus_tag="IWO38_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0314024.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            18272..18919
                     /locus_tag="IWO38_001939"
     CDS             18272..18919
                     /locus_tag="IWO38_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0314025.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            18924..19907
                     /locus_tag="IWO38_001940"
     CDS             18924..19907
                     /locus_tag="IWO38_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ0314026.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMKANKNIGKIILKITE"
BASE COUNT         6217 a         4302 c         3502 g         6158 t
ORIGIN      
        1 taaaataaaa aatttattgc aaaaaaaata gaagtgacaa aattatcaat catttcaagg
       61 taatataact acctgagttt attatcgatt aaaatgctaa ctacatttgc aagagaagcg
      121 agagttaaag gtaaatatag aatttctact tttttagcaa tagaaatagg aataaaatat
      181 ttaaaatgta tagaaacttt cttttttttc ttgctatctt tttataatca caactctcta
      241 taatagaatt acgatttatt ttatacggct gaaaaaatct atcaccaaaa aaattcaaac
      301 attaagccaa ggattatgaa gtcaacattt cgaaaaatga acatacattg gtaagcaact
      361 ataactatta ccacagacat aaagtaacac aatatattac tatttaagtg tacgccacca
      421 gtgaatatcc tcaaggtata cagcgcacaa caaaaagaaa aaaggaaacg cagaagcatt
      481 tcctttcagt gatttaatga aaacaacacg atttgactta gagccttttt atttgaattc
      541 attttcgctt tatccaactt catttgatca tttgattact cggttaaata cttttgacat
      601 gagtatcaat gaaacattca ttaattatgg ttgcttattt cttaccgctt ttctggccaa
      661 tgctaatccg ccgattattc cggcattatc acctaattcg ggcgtaataa tgtagctatc
      721 aattggtggt atttcaacat atccatttat aagctcaaca aatttatcct gaactttttt
      781 cttcatgtgt tcttgtttca ttacaccacc gccaaaaata attctatctg gcgaaaacat
      841 cagtgttgta ttgtatgcac attgggcaag gtaaaaggct tcaatctgcc aaaacgaatc
      901 gtcagccaat aaattctggc cttttacacc caaccgtttt tcaatcgctg gtcctgctgc
      961 catcccctct aaacaatctt ggtggaaggg acagtttcct gaaaaattgt cctttggatg
     1021 tcgcttgatc aacatatgcc ccatttccgg atgactgaac ccttccacga aagtcccatt
     1081 gaacaaggca ccacctccga tacctgttcc cacagtgaag tatgcaacac ctgctaaccc
     1141 tttgccagaa ccagcaacat attcaccata gcaagccgca ttgacatctg ttgtccattc
     1201 aatcggaata ttgaaatatt gctttagcgt tcctacaaaa ttgaaatttt gccaagcaag
     1261 ttttggtgtt gatgtgatat gaccgaaact cgttgatttg cattgaatat caattggacc
     1321 aaagcagccg accccgatgc ctgccaattg ttccttaaaa ggtttaaaaa aatcaatgac
     1381 ctgcttcatt gtttcaactg gagttgttgt gggaaaactg actctttcca aaatcgttaa
     1441 ttcctcagta ccgactgcac agacaaattt agtccctcca gcttcaattg ccccatatat
     1501 catcataggt tcctcccttc gaatatgtga aaaacacttc attaacgcgg caagcgttct
     1561 cgatcagtga aatgacgttt agtatttcct gaagcttctg ttgaatacca gtaagcgaca
     1621 ctagcaacat cgtcttgccg ctcaaataag cgaacatcat cgttgccgat ttgttgtaaa
     1681 gtgaccgata aatcttcggt aaaacagatg gggtcttgaa gatgccaacg ataaagacca
     1741 tgtcgtggca ttgcgtcgtc accaaaggca tgaacggcat tcaattttcc cgtttcaaaa
     1801 cgatctcttg tggcatctct atttgattgg taaggatacc ctaaaaaggc tgttgaatag
     1861 tttttcgcat gtggacgtcc gaactcatcg tactcatgaa aggcccacgc cccgccaaaa
     1921 taatcttctg aacctgttga ggttacggtc ggatactcac tatccccatc aatgtagaat
     1981 ttaaactcgc cttcgcccca ccaataacgt tctaggcagg taagcgcaag gtaagtaccc
     2041 acgtaatagc cttgaccctt taatcgatca attaacttat aatcctcctg taatttggta
     2101 atgcgttccc gatgccaatt tgcgtgaaaa tataggctat tttcttccaa ttcgtccacc
     2161 aaagcgtaat ttatcgtata aaaaaatgag gacatatcct tcggatgtaa attctcgatc
     2221 gtaattttag cggatgtttg gaaaggcatt tcgaaatagg aattaaagcc ccctgtagga
     2281 ttaaccacta tcggcaacga gttcacctcg caacgctctc caaacccatt acaaaagaaa
     2341 tcccctaaag gactttcaac agcagggcta tccgacccat cccaataaat tcttaagatt
     2401 aaatcacgta aaacaaaact gccaaattct gttcgatcac gaatcgtcat ccacatatga
     2461 cgaattattc ccgttccagt aatatccgca atcacagttt cttcaccaga agtcagcgga
     2521 attgcgccac gacctttgcg gcttggtcct agctcgcttg gcaacatcgc agctttacct
     2581 ttctcgcctg ttggattttc gggagtgatt gctcgactct ggattttttt aaattgggta
     2641 atatttttta gtatactcat ctttttctcc tttgaatgtg aatcgttttt cccttttcta
     2701 cgtgaagaat ttttttcgta tcacttattt tcaatggctt ttctgacaaa tttgaaatac
     2761 tcaactcgga ttgcgtacat tcaatcgaaa tgaaggcttg accgaacttc aaattcttcc
     2821 aagaaaacgt taaattatca acaggatcaa tttgcaatac tccttttttg aatttcaatc
     2881 cgaaatattg actgatcatt aggctgacaa aaagacccga tgcccagccg catttgccgc
     2941 agccaaagtc tcgcacgact tctccttgtt gagcctctaa gcgatacggc caccaccacc
     3001 acgaaccgtc taaatctgcc aaacaaatta gttcttctaa tcgggttacg aaagcttgag
     3061 ggttgtgaag ctcgctcatt aaaacagtga taaagccagg aaaagtggcg ccggattcta
     3121 atccccaagt gatgcctttg atttcctcgc tataggttgg attgaaagaa ctagcagaaa
     3181 aggccatggt gttctgatat aaatgatctt tattatctaa aaattgataa aaaggcatca
     3241 acgtagtgtc tgattcttct ccatcatgca tcaataaatt aatttttcca tccacaatca
     3301 catcacttaa aaagattaac ccttgctcaa gaatcggttt ttgatagtgc ttcactgaat
     3361 aggttgtttt ctcctcatca ccgattccct ccaaaaattg ctcaccaaaa ggaccatcga
     3421 tagtcatttg tttacaaatt gattccttta gtttacaggc aatgtttttg tatcgcttgc
     3481 taagactcag ttgttctagg gcttcaaaaa tttctgcaag tccaatacag gccttccaaa
     3541 gacaaagatt gctgccggta tgatactttc caagtgcaaa ggcgtctgat aaccaaacag
     3601 atggaaaaag cattggttcc cctgaccgac gttgagctaa tatgtcctca ataattcttg
     3661 aagcattcag tagaacctgc ggacaccgct caaagaattt tgtatcccca gtatgttcaa
     3721 aataaagact agccagtaac gcactggata acgaattact cagagaatga ttgatacctc
     3781 ctgtaaattt tgaaccagga aacttaacgc catattgatc aaaccataga accgtttttt
     3841 gacataactc cggttcaaac atggtgaatg gcaatgatgc gtaatacata tccttattcc
     3901 aaattcgatt agtagcagga tagctgcccc aattgctgcc gacgacctgt ccctgtcgat
     3961 tcataccgaa actagaaaag ctttgataaa gtgcacgacg atagagatgg acaactctat
     4021 cgtttggcat tgacagctgc ccaaatagct gagcaaaata gttcaatgtt tccttcagcc
     4081 atttttcgat atcagattcc tgaaaagtca ttacttcttc aaaagcattt gggtccacaa
     4141 aagcaatggc gaagcttgcc acttcctgtt gtgcgatcgt gtccttattt cctttttgag
     4201 aaatcaacac attttgctga tccgaatact tctcttgata cgaaggcaca tctttcactg
     4261 aaacttgaag gctttcgtgt gtttcattca caatgtaaac ctgttgaaca agcatactta
     4321 aacgcttatt agccagtatg ggagcaaagg atactacaat gaccttaaac gtcgaataat
     4381 gatgaatata tctaggcaaa cagtcttgaa tcacatcgac ttcaatacga tgatccgatt
     4441 ctgacaaatg gtagacaatc ccgtttactt caacctttaa ggataacgaa ttggtttgag
     4501 agagctgttt attgacccaa acacctggtt tttcctcagt tagtaaattt tctcgataaa
     4561 aggtcaacgt ttgaatcgtc ccgcaaacat caaggtttgc taatgtcttt tgattactta
     4621 catcgaaggt caatcccttt acatcaggaa tattccgata gtagctgtat tcgtttgaag
     4681 tagtcacatt tccgtcgttt aactcagcat taaaaaatgg gtgtttgggt gaataaatta
     4741 cttcattaat agtcatcttt tccctccttg ctaaaaaaac tgcagcctag tagctgcagc
     4801 ttcctactat gcgattccaa gtaatgacaa taaaattccc aagacgacca ttccaaaaat
     4861 aattttgatg acgccgactt ttttatctag taatttgaaa cagctcaatg ttaccaataa
     4921 cggtacaatt cctttaaaaa tactatcgag gatttcttgg acattcatta gttcttcgcc
     4981 cttcattgaa aagctcaaaa cggtattgaa tttgaccata ctgatcgtca ttgcacctac
     5041 catgatcaga cccaagatac tagcagcttt tgtcagaatt tcaattaacc cgttcgcata
     5101 cagcttttca atatatttag agcctaatga atagcctaaa acgccgccat agtatcgcat
     5161 caagtaatta ggtatattga acataactaa aaagataatt ggtgccaaaa tatttccggc
     5221 attggctaag ttgatcgcta gtcccgcggt aacgacacgc caaactcccc agaacaatga
     5281 atcgccaatt ccagccaatg gtcccatcaa actcgttttg atcgcgttga tactttccac
     5341 gtcaaaacca gctttttcag aattttcctt ctccatcgat gccgcaatcc ccatgacaaa
     5401 ggacgcgcaa ggaatggtcg tcgaaaaata ctgtgtatga cgttccagcg ccgcagctcg
     5461 atcttcttca cttttgtaga aatgattaat gaagggcatc atagaatagg cgaaaccacc
     5521 gccgcccatc gtcaccggat tcaccgaagc atacatagtc atcgagcgcc aaaacatctt
     5581 ccgaaccaat tttttctcat ctttagaaat tccatcagct aatttcattg aaagaaatcc
     5641 tcctcttctt ggtcaagcgt tgcatttaca gcagcatcgc tttgaactgc atgaacagat
     5701 atatttttta gttgtagatc tctttgggct ataacaattg caataatcgt tccaactgcg
     5761 gcaattgcaa tcaatggtaa ctcgagatac gccataacaa cggccccaaa gaaaaaccac
     5821 acagcaatat ttttactcca aagcatccgt aacagcatcg ccatcccaac tgccggcagc
     5881 agatttccag cgactgttaa tccgttcatg atgacctctg gaatattatc caagacgctt
     5941 ttgacaacat ctgctccaaa taaaacggca aagaatggaa taagagcata aagcgcatag
     6001 tggacaaacc acataaagaa gtgcagattg atgattcctt tttgatcacc agattttgca
     6061 aatttgaaaa atttagcagc catcggtcca catacaaaag aaaaaatcat attcattaac
     6121 tgtaatccta atactgctag tggaaagaca atgggaataa tcgcatccac gtttcctccc
     6181 attttgattg cgaacgctgt agccagccca gcagccaaag aaggctccgc cgtactagag
     6241 attccgatat tgacgacacc cataaagatg gcttctagtg acgccccgac aatcaacccc
     6301 gtctgcaaat cccccatcaa cgctcctgct aacggagcaa caacaatcgg acgactcagc
     6361 atggacatcc cgaacatttc atgtccagca actccaataa aaacggccaa agcgactaca
     6421 aatgcatcat acataatttt tctccttcct ttttctaaaa atcactcaaa atagagatag
     6481 ctgaacgttt ttccatcgcc ggtgtaggct gaaaaacggt atctggataa agttcaacta
     6541 attcttttaa gtcttgaatt tctgacgtcg ttaacatcac ttcctttgat aaggttttct
     6601 tatcttccga tggcggtgtc attttcccag cattccccac gttcattaat tcgatttccg
     6661 ttgggaaagc tttcgcaact tttaccgcat ctttgacatt agggactaaa accatcaagc
     6721 gcatgtcctt cgcccgcgga tcttttaaca aatgaatcgc gtcatcaact gttttaataa
     6781 tgactttgac attcgatggt gcagccatct tcaatgtcat cttttgaata tcgttggtag
     6841 ccgcctcatc gcttgctact actaacccgg ttaaatttag actctttgtc caagtcatcg
     6901 ctacttgtcc atgaattaat cgttcatcaa ttcgcactgc tttaatcatt tttcttcctc
     6961 ctaaaaaaaa tcttcctctt catttactac atgtacctca tcaagaatgg tcatctttaa
     7021 ttcctgttgg catttattta ccagttcctg aatatcctcc tttgttaatt ccttggttgc
     7081 caatactatt tcgagcagaa ttggcagatt aaagccagac aaaatagtta cagcaatctc
     7141 tttctccaaa aaggttttgg taatcttctg attgacactt ccgccaaata aatcggtaaa
     7201 aacaaaaatc tgatcctgct cgttcacctg gtttacaaat acattcaact cctgttcaaa
     7261 atcacactca tccacataag cattaatcac cgtaatctct tgaaccttcc ccgtcagtaa
     7321 ttgaatactg ctaagaaagc ctgaagctaa gtgaccgtga gtcgctatta gaattttctt
     7381 catatggtta attcctttct ttctgttcac ttttatataa gcaattcctg tgccaacttt
     7441 taaaaacatt ggtttaatca cgttaattca cattaaaaaa agtgtctaac ctaaaaaaaa
     7501 cagattagac acttttttag acactttatt tttgaatata cggtcctaat aattcataga
     7561 gcagcgaaag ctcataagca ttgactttaa aattatattt catctccatt gattgaaaaa
     7621 tacttctaga aacataataa aattcatctt catctggttt caaatcataa ttcggttctt
     7681 cagatttttc tgtccgagcc aagaataaac gttccatcat caatgccgta tgcatataga
     7741 gatttagttt tacacggcca tttaaagtga aatgatagta attttcatat ttagaaatga
     7801 ctgtttccac ttcactcatc acgatcgttg ggttcagaaa gttcaaacgg tccgcaattc
     7861 cttccaacga aaagaatttt actagctcct gaatcaagcg gttaaattct gccggcttca
     7921 tttcaggact caaaatatgc tccaaattgg ctgctccatc accgtctaag atgtcataaa
     7981 cattaatatt aggaattgaa aaggaacgtg gaagatccga agtcgtgatg accaattttg
     8041 tcttgtcaaa ataagtttca tcattttggt cgattaattc acgtagttct ctgtagtcct
     8101 tcgtgaaaac ttcgatggat tcatccgata aatgatgacg aaaaacatct ttcaatttgt
     8161 ccgaaatccc caaacccgac atacaggaaa tgataatgtt gctactttga gaaaagcctt
     8221 caaagtattg aacattgatt gaatacttgt ctgctgcatc ctccgcaatc tgtttaaatg
     8281 gcgcattcat cgtcatcttc aagccaatat caagtgcaat ggaagtcgtc aaattattca
     8341 gaagaagtaa ctcccctttt aaatcgttct tgatctgtgt gtaaatttga tttaaagagc
     8401 ccatatctac gatcagcact agactatctg tattcacttg ccgtttcacg aaattccttg
     8461 tcgattcgac aatctctgac acattactct ctactggcat atccaacgct tcaaaaacat
     8521 aattgccgca taattgattc actactgctt gaatgctgct agccgtacta tctccgtgag
     8581 caagcaacag gcccttcagc tcgatggatt catctacata atcagataaa gtcacgacaa
     8641 taagcagtct tagaaagttc tcgtcctttt ctgagagcgg tggtaaatgc tccaagaact
     8701 gattacataa gtaagtcgtc cgaggtagat ttgcagctaa tgtacgattt atcccggtta
     8761 agtctgtctt atagcttggg gaaatgacat aggatctgct aataatttct gcaacaggtc
     8821 cactcgtcgt caatccgtac gtttttccta aaacggatac ccagaccttt ctaaacatct
     8881 gaatcatcaa ttttctactc attgataaaa aagaatcctt atcaatgtga tgcaccaaat
     8941 gctgtacttc attctttatc gaagaaaaat tttcattgaa attaccttca tttatcagct
     9001 gagtaatttt ttccaaaatc gaatcataat caaacggttt gattcgatcc gtttttatct
     9061 tttgattttt ctttatcaat aaactagcaa tcggcaagct ttctacttcg gattccagca
     9121 tatttaactg aatcaagatc tcatttttcc caatcgttaa tggttcttct tgtgttcgac
     9181 tatacacatc cgcacaactg attttaatga tattcttcag cttaccaata ttcccactaa
     9241 agggatgaga taacattaaa gacaaggctt ctcttgaaac aataatatct ttttgtaaaa
     9301 catccgcttc attttgaaaa aataattgaa tcagctctaa tcgttctcga atggggcgat
     9361 cttcaaaagt tggcaacata acactaattt gaatacgccg atcaaaggtg tctaataaat
     9421 aattttcacc tttttccgtc gtggcgaaaa taaaacgaac cttagcagaa tgccaattta
     9481 cattttcacc gactggacga tagtttccag tatcaataaa tagaaagagc ttttcttgat
     9541 tttctttcga taggcgatgg acctcatcga gaaaaagata gccgttgttg gccgttgcca
     9601 ataaaccatc ggtatccttt tcagcccccg taaaggcccc cttcttgtat ccaaacagtg
     9661 ttgccgaaag cagttcggga ttgtccgcat aatcggcaca gtttaagata gcaaatggtg
     9721 catctttttc aataatttgc tcatggatag cgtattcaaa aatttttgtg gctaaaaaac
     9781 tttttccaac acccgtggct ccatttatta atacactgag tccgttaggc ggatatttga
     9841 cagcggctat acatttttga atgacctctc tcagacttcc gtcatgacca actacagaat
     9901 gaaaggcttc gtttatttgc tccgtattat ctacaaccgc atattcatct aaaggcttcc
     9961 acaaaacagg ctttcccggt aatttctcga cctttttatc ttccactaat ctcgtcaaat
    10021 aatggctggc attctgacga gagataccta gattcttcga aattttctcg gcagtgacag
    10081 aagacatatt ccctgttagt cgtatcagct catcaaaaac tcgatcttta gcgctctttt
    10141 tcattttttc ctccagttta atgtgtctaa aagcataata aactatcggc tgcaatatgt
    10201 ctattctata tttgtattcc aatttttact ttgaaacaca aatagtcgaa tatttgcctc
    10261 agatatctga caaaataatt tatctaccaa tttagttttt atctaatatg atttttatgt
    10321 tttttcatag ctacaaatat tgaactggat tcatatatta gttttattaa tagattacag
    10381 aagggttaag tatatttgca tagtttccaa ttactttatc cttctgtaac tttttcaatc
    10441 ttagatttac tagcccgtct cgggccctat tttacaatgg ttttgtccac ttttctctgg
    10501 tagaaaaacg tggtagaaaa aataaaataa cacaaaaaaa gcggcgtttc atccgctttt
    10561 ggtagaagat tggaaaactc agatagaaaa aggacgattt ttagctcttt agaacgttga
    10621 taagtcccca ttcttactga gttttgccat tatctcctat acatcttgaa ttctaagaat
    10681 aggtcattat agatgcctag gtatttcgat cctagatctt tgtagacttc aagatgggag
    10741 atcgtgtcat cagatggata gaattgttcg tccgacgaaa tgtcttttgg caataacgct
    10801 tttgcttctt catttggtgt agaataacca atatactctg cattttgtgc cgcattttcc
    10861 ggttctagca taaagttgat gaaatcatag gcaccttctt tatttttcac ggtttttggc
    10921 ataacgatat tatcaaacca aagattggat ccttctggtg ggatcacata atgtaaatgt
    10981 tcattttccc acatcatatc ggcagcttcg ccagaaaatg tcactgccac ggaggcttct
    11041 tcattgatca tatacatttt gatctcatct gccacgattg ctttgacatt tggtgttaaa
    11101 tgattcaatt tatttgctgc ttctgtcagc tgtgccatat tcttactatt caaagaatag
    11161 cctaaactat tcaaagaaag ccccataact tctcgtgcac cgtcaatcag catcaaactg
    11221 tcttttaatt ccggcttcca caaatcatcc caatgcttaa tttgatttgc atcaaagaat
    11281 ttatcgttat atacgatccc taaagttccc caaaaatatg gaatcgaata ctgattatct
    11341 ggatcaaaat ccaagtctaa aaatcgtgga tcaatattat ttaatccttt gatcttcgaa
    11401 tgatctaatg gaagtactag tttttctttg atcatttttt ggatcatata ttcacttggt
    11461 atagcgatgt cgtagtttgt cccaccttgc tggatttttg tgaacatcgc ttcattagaa
    11521 tcaaatgttt cataattcac tttgtatcca tattcttttt caaatttcgt gatcaaacta
    11581 ggatcgatat aatctcccca gttatagatg gtaagtacct ttgctccact catgccactt
    11641 gttttttcaa gttgacgaac accaagcagc agcaccgcaa taatgatgag aatacctaaa
    11701 aacagagatt gtaatttttt catcgcagcc gtgccacccc ctcgcgtact cttctgtttt
    11761 tcttcagccg ttttggacgg ttttctttac taataaagta gtatccaatg accaatatca
    11821 tggagaacaa gaaaactaac gcacttagcg cattgatttc taaactgatt ccttgtcttg
    11881 cacgagaata gatctctact gagagcgtcg taaagccatt tcctgtgacg aaaaatgtaa
    11941 ccgcaaaatc atcaagagaa taggtaaatg ccataaaata tccagctatg attcctggag
    12001 aaagataagg caaaatgata tttttgacta cttggaaatt attagcacca aggtctctag
    12061 ccgcatcgac cattgaatcg ttcatttcct gcaattttgg caagaccatc aagacgacga
    12121 ttggaatgct gaaagcaata tgggaaagca atacactctg aaaacctaat ccgaatccta
    12181 gtagtgtaaa gaaaatcaag aaactagcac cgataataac atcaggagag actaataaga
    12241 tgttattcat gctcaacaac gctgtccgtg cacgtcgttt tttcgtgtaa tagattccca
    12301 tagccccaaa tgtcccgatg atcgtggcga tcaatgcaga taagaaagcc agcatgaacg
    12361 tattcaatac gataatgatc aaacgtgtat cttcaaaaac agcttcataa ttttcccaag
    12421 taaatcctgt aaagctgttc atcgttcctc cttcattgaa ggaataaaaa atcagataga
    12481 agatcggtgc atataagaat aaaaagacga tccctaaata aagatacgac catttaaatt
    12541 ttttcattta tgagcgacct ccttttttct tctcgcctgt caggagcatc acgatgaaca
    12601 tagcaatgat taaaatcaca ccgattgtag aacccatccc ccagttttgt gtaaccaaga
    12661 aatgctgttc gatagccgtt cctaaagtga tcacccgatt tccgccaatc agacgagtca
    12721 gcatgaataa agaaagagaa ggaataaata ctgcttggac accacttttc acaccattta
    12781 atgataacgg aaaaatcact cgtcggaagg tctcaaagct attcgcgcct aaatctctac
    12841 tagcactgat caaagaagga ttcatctctt ccaatgcatt gaaaattggc ataatcataa
    12901 atggaatctc gatatacgtt gccacgaaca gaaaactgaa gtctgtgaat aatatctgtt
    12961 tcggcccgat cccgataaaa tcaaagaaat gattcacgct gccgtgcaca ctgaaaatcc
    13021 caataaatgc gtaagcttta agcagcagat tcacccatgt aggcaaaata atcagcatca
    13081 gccataattg cttgtgcttc agttttgtta aaaaataagc agtcgggtaa ctgatgatca
    13141 gcgtgaaaaa cgtgatcaaa aatgcatacc atacagaatt gatcgtcata gaaagcgatg
    13201 tccccgacgt aaaataagat agataattat tcagcgtgaa ttgcccattc atatcaaaaa
    13261 aagattgata aatgatcatt aatacaggcg cgatcacgaa taaaccgagc cacaaaatat
    13321 aagggacgga atagatcctg cgcattgttt tcattcttgt cccccctatt cttcgtagct
    13381 gtcaagacgt gcatcgaatt cttcttcaga ctcgttgaaa cgcatgacat ggatatcttc
    13441 tggctcaaag gccaatccta ctttgcttcc ttctttagct tttttcgttg aatggaccat
    13501 ccattcattt tgctgttcat cataacagat gatttcataa tggacaccac gaaacagctg
    13561 agtatcgacc gttacgacta gttttccttt atctggtgtc gtgatcgtca aatcctctgg
    13621 gcgtaaaaca acttcaactg gttcatttag acgcatcccg ccatcgacac attcaaattg
    13681 cttaccaaca aactcaacta gattatcttc gatcattaca ccgtccacga tattgctctc
    13741 cccaacaaaa tcagcaacga aatgattgat tggctcatca taaatatcta cgggtgtacc
    13801 actttggacg attttccctt tattcatgac aaaaatctca tcactcattg ccaacgcttc
    13861 ttcttggtca tgagtaacaa aaataaacgt gattcctaat cgttgttgca gatcccgcag
    13921 ttcatactgc atatctgtac gtagtttcaa atcaagcgcc gataacggtt catctaatag
    13981 caatactttc ggttcattta caattgctcg tgcaatcgct acacgctgtc tttgaccacc
    14041 agacatttca ctaatctccc gtttttcgta tccaggaagc tggaccatac gcaaagcatc
    14101 ttttaccttc ttttcgatat ctgatttgga cattttcttg atcttcaatc caaaagcaac
    14161 gttatcaaaa acattcatat gaggaaaaag tgcataatct tggaagactg tatttacttg
    14221 tcgcttatta gctggcaaat cattcaaccg tttcccttca aaatagatgt ccccttctgt
    14281 agcttcggtg aatccagcta taatacgtag gatcgttgtt ttaccacagc cggaaggacc
    14341 aagcagtgta taaaattttc cttgttcgat ctcaaaactt acttttttta atactgtttc
    14401 atcatcatag cgtttcacta cttgatcaaa tgaaatgatt ggtttcccca cgtttttacc
    14461 ccctatatta caaatatgat tgcgttgcaa caatcaatac ttttgttcgt tttttgagat
    14521 gattccttaa ttgatgttgt tttgtcgctt gataatagat cgactgtcct gcttttgcca
    14581 catagaccgt ctctcctaag cttaactcta attccccttc taatacaaaa ataaacgttt
    14641 cagataatga aggttcaaac gtcttatatt ctcctgcacc ttcaaaagtc agcaagacag
    14701 gctccatttc tttttcattc gaatcagcca cgagccattc caattcatac ccgttttctt
    14761 catcgaaata aaccgtatga tcttcttttg aataaaccac ttgcagattc gctgcttctt
    14821 gatgaaaaaa ttcttctgga gtcacaccta agacttcaag aatagaaaag aatgtttcca
    14881 ttgatggtga acttaaatct cgttccaact gggaaatata cccttttgac aaatctgtgc
    14941 gttcgcctag ttcttcttgt gtcaaattct tttggatgcg caaatttctt aacttctcac
    15001 caatttccat aatttcctcc gaatcgaagt tttctaatag taaacttcct gtttactacc
    15061 actcgtatta gtatacgaaa tttattgaaa aaggcaaccc tttgtaaaat tttttttgct
    15121 gaatatttct ttatcttttg gaataaattc tagttgtttg attctttatt tttcacctaa
    15181 tttttgtcta aatttacgga agaacaagaa taaaagagta ggctttatgg tatgataaaa
    15241 gaaaatttta aaaggagtgt ctcacgaatg aacgcttttc gatcttttca ggtaataaag
    15301 gaacctgttt tccatacagc aatcagtgat ctgccattta ctgagttggc tgaaaatgaa
    15361 gtcttggtaa aaattgccta ttctgatgtc aattataaag atgcattggc aatgagcgag
    15421 tctggtcagg tgatacgaac atatccgatg actccaggga ttgatttatc cggaactgtc
    15481 gtccaaagta acaatccacg tttttcaaaa gaagatcttg tacttgctac tggttttggg
    15541 cttggcgtca cccatccagg cggttatagc caatatcaaa aagtaccagg agactggtta
    15601 gttcctctac caaaaaatat gacgctgcgt caagcgatga tccttggtac agcagggttt
    15661 actgctatgt tgtgtgtgaa cgctttaatc cgtcaaggta tgacctccga taaaaaagtt
    15721 gtcgttactg gtgcttctgg cggtgtaggc agtacagcga tagccctgtt acataaacta
    15781 ggattccagt caatcattgc cttttccaga aaagaagagt ctgtcacatg gctaaaaagt
    15841 ctaggtgctt tgcaagtcgt acgacctgat gaatttctgc cagagacaac aaaagcttta
    15901 ggaaaacagc aaatcgacta tgtcatcgac acagtaggtg gcgagcagct aagcagtctt
    15961 cttccattga tctcttataa cggtgcagta gctttatgtg gcaatgctgg gggaatcaaa
    16021 ctaaacgcga ctgtcttgcc ttttattctg cgaaacatcc agttgattgg aattgattct
    16081 gtcaatgtac caatagatca acgtctttct ttatggcaac agatggcgga tttgcagatt
    16141 gcagatgaac tggtcgtcca agagattact ctggatcaat tgcctgaaac agcatctaaa
    16201 ctacttgctg gaacacacca aggaagaaca ctagtaaatg taggtgacca taaatgaaga
    16261 ttctaatcac agctggagga acgactgaaa aaatcgatca agtacgtgct attacaaatc
    16321 attcgacagg acgattaggg caagcattag ctgattattt agctgccaat ccggatacca
    16381 caatcgatta cgtgactacg aaacaagcac tgaaaccgaa aagacactcc aatataacga
    16441 tttatacgat cgaatctgca ctggacttat ttttacaatt agaagaacta acgaaaaaag
    16501 aacactacga tgcgattatc cacagtatgg cagtcagtga ttttactcct gcttttagtt
    16561 tttcagaaga acagctggca aaaaacctac ccacttcttc aacgcaagaa gaactggaca
    16621 attggtttgc ggaaaatgaa cagacagata ccactgtttc aaagatctct tctaacaccg
    16681 agcatcttgt gctcgttttg aagaaaacac caaaaatcat ttcttatcta cgtgaatggc
    16741 agccaaaagc aaaaatcatt ggtttcaaac tattagtaga tgtaccaaag gaatctcttc
    16801 tggcagtcgc aaaaaattgt ctgataaata acaaaactga ttttattttc gcaaatgatt
    16861 tgacggaaat ccatggcgag acacaccacg gctatctctt atctaaagat ggaacagtgg
    16921 aagaaactca aagtaaatct gagattgctg cattgattac tgagaaaatt agactggagg
    16981 aatcaaaatg aaaaagatct tactaggcgt tactggaagt atttctgctt acaaaagcgc
    17041 tgatcttaca agtcaattcg tcaaattagg tgcccaggta gatgtgatta tgtctaaaag
    17101 cagcacacat tttatcacgc cattaacctt gcaatctctg tcgaagcgcg cagtccatac
    17161 agacgtcatg caagaatcaa atccgcaggt aatcaatcat atcgaactag caaaacaagc
    17221 tgacttgttt ctcatcgcgc ctgctacagc aaacattatc ggcaagctag caaacggcat
    17281 tgcagacgat cttctttcaa cagtcgcaat ggcgcttcca gtgggaacac caaaattgat
    17341 tgcccctgcc atgaacacga atatgtatca aaacccgatc actcagcgta atctggaaac
    17401 attgagatct gtcggctatc aggaaattga accaagagaa tctcttttag cttgtggaga
    17461 ttttggaaaa ggtgccttag ctgatattca gacgatcatc gaacaggtca tgctgactct
    17521 taatgaaaga aatgcttaga aaaaaggagt acttatgaaa aatacaaaaa actttacatt
    17581 aactgcgatg tttttagcta ttttgatttt attagctgtt acgcctttag gttttatccc
    17641 tatcggtcca atcaatgcaa caaccatgca tattcctgta atcattgctt cgatcgtttt
    17701 aggaccaaga ctaggtgctt ttcttggcgg tacatttgga ctgatcagca tgatccgtag
    17761 cacagttatc caaacacctt tgtcttttgt cttttctccg tttatcccag tcatcggaac
    17821 agaacatgga agctggaaag ccttgctgat cgcgtttatt cctcgcatct tgattggggt
    17881 ggttccttat ttcgtttata aaggtctgcg caaactaatg aaacaaaaag cagattccgt
    17941 ttctttattc ttagctggac tttccggttc attgaccaat acgattttag tgatgaatat
    18001 gatctacttt ttattccagc aagattatgc caaagtcatc ggaacaaatt taaatgctgt
    18061 ttattcagct attctagcgg ttatttttac aagtggtata cctgaaggaa tcgtcgcagg
    18121 tcttgcgact gcagcagtat gcaatgtctt gttaagatac ttcaagcacc cttctcttac
    18181 taactgatag tcatcgtagc cgatctctga tttttttctc ttttggtgtt actattgaat
    18241 ggaaagattt gacgtcgaaa ggatgatcca aatgaaaatt tttgttgtag gtgcaaacgg
    18301 acaaattggc cgacacctga taaaagattt agctccaagt tcccatgaaa tttttgcagg
    18361 ggtgagagat gttgcttccc aaacattagt aaaaaaagag aatgtttcgt atgtctcttt
    18421 tgatttaact tggtccgtgg aaaaaatgac cgaagcattc aaaggcatcg atgtattgat
    18481 ttttgctgcc ggctctcaag gaaagaatct tctgcaagta gacttggatg gcgcaatcaa
    18541 aacagtgatc gcagctgaaa atgcgcatgt ctctcgctat ctgatggtca gtgctgttta
    18601 tgcagatgaa ccagcaaaat ggcctgagtc catgacagat tattatatta ctaaacatta
    18661 tgcagatgaa tggctgaaac gaactaactt agattttgtc attcttcaac cagtcacatt
    18721 gactaacgat gaagaagtta cttccattca attaactaag cctaatgaga aggcatcaaa
    18781 aacgatcact cgctctacag ttgctgctgt acttgccgct ctagtggaag aaacggatat
    18841 ttctcgtacg actctagtac tttctgaagg tagcaaagag ttgaatacag cattccaaga
    18901 atgggcaaag gaggaataga tccatgcgtg ccatttcaat caagcatcct ggttctgtag
    18961 atgtattaga acaaattgaa gtgcctgttc ctaaaccaaa acaaggcgaa ttactgatca
    19021 aagtccatac agcagctgtc aatcgaacag atattatgaa aagagaatca gtctctttga
    19081 aacctcccta ccctattttg ggagtcgaag tagctggaga ggtagttgag aataaaagtg
    19141 atatctccac ttttactcct gggacaaaag tatacggatt agtcaatcta ggtggttatg
    19201 ccgaatatgc tgtgatgcct gccgatcgtg caatccttct ccctgatacg ctagactatg
    19261 tttctgctgc tggaatctca gaagtttttt tgactgctta tcaaacactt tactggcttg
    19321 gaaaattaca agaaggcgaa actgtcttga tccatgcagg agcaagtggt gtgggaacag
    19381 cagcgatcca attagctaga cagctttctc acgcgaaaat catcgttact gcaggatcag
    19441 aagaaaaact cgccttttgt aaagaacttg gtgcagatga attgatcaac tataaaaaac
    19501 aagactttca agaagaggta cgaaaaatca ctgaaggtcg gggagtcgat gttattttgg
    19561 atttcatcgg cgcttcttac tgggaaaaga atttagcttc tatagccgtt gatggacgtt
    19621 gggtcttgat tggtatgcta ggtgggacca tcgtgcctga gatagacctt ggtgtattga
    19681 ttgggaaacg tgtccaactg cttggtacat tattgacacc tagaagtgat cagtataaag
    19741 cagagctcgc acaaactttt atgcaaaaag ctgggccttt tctagaaaat ggcaagattc
    19801 ggccaatcat tgaccgtaca ttccaattca gcgcagtcaa acaagcgcat gaatatatga
    19861 aagcaaacaa aaacatcgga aaaatcattt tgaagataac tgaataaaat catgctgatc
    19921 aaaaaagagg ctgggacaga agcgtttaac tccaagaaat aagaagaaat tcacgaaaat
    19981 tgcttttcaa atttttgtga atttcagctt atttccgaag gaatttcttc tgcttccgcc
    20041 gtttatacgt gtttagagcg tgagacaaaa gtgttcttta cttttgtccc acgctctttt
    20101 tccgaataaa cggtgttcaa aagcttgcat ctgcggtaat aagtccaacc aagttaaaaa
    20161 cataagaagc tgtttttac
//
LOCUS       DACECY010000049        20172 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.053, whole genome shotgun sequence.
ACCESSION   DACECY010000049 DACECY010000000
VERSION     DACECY010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.053"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            42..197
                     /locus_tag="IWO38_001941"
     CDS             42..197
                     /locus_tag="IWO38_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314027.1"
                     /translation="MVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLLNL
                     FNRIVGA"
     gene            210..713
                     /locus_tag="IWO38_001942"
     CDS             210..713
                     /locus_tag="IWO38_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0314028.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            840..1877
                     /locus_tag="IWO38_001943"
     CDS             840..1877
                     /locus_tag="IWO38_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314029.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            1892..3589
                     /locus_tag="IWO38_001944"
     CDS             1892..3589
                     /locus_tag="IWO38_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HAQ0314030.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKFPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            3582..4043
                     /locus_tag="IWO38_001945"
     CDS             3582..4043
                     /locus_tag="IWO38_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314031.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            4056..4916
                     /locus_tag="IWO38_001946"
     CDS             4056..4916
                     /locus_tag="IWO38_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314032.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            4954..5343
                     /locus_tag="IWO38_001947"
     CDS             4954..5343
                     /locus_tag="IWO38_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0314033.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            5330..7777
                     /locus_tag="IWO38_001948"
     CDS             5330..7777
                     /locus_tag="IWO38_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0314034.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            7782..9890
                     /locus_tag="IWO38_001949"
     CDS             7782..9890
                     /locus_tag="IWO38_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0314035.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            9887..10891
                     /locus_tag="IWO38_001950"
     CDS             9887..10891
                     /locus_tag="IWO38_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0314036.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            10910..11821
                     /locus_tag="IWO38_001951"
     CDS             10910..11821
                     /locus_tag="IWO38_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0314037.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            12008..12295
                     /locus_tag="IWO38_001952"
                     /pseudo
     CDS             12008..12295
                     /locus_tag="IWO38_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(12483..12698)
                     /locus_tag="IWO38_001953"
     CDS             complement(12483..12698)
                     /locus_tag="IWO38_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314038.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(12957..13124)
                     /locus_tag="IWO38_001954"
     CDS             complement(12957..13124)
                     /locus_tag="IWO38_001954"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0314039.1"
                     /translation="MGSECGHTDGKKSFTIREWTCPICRTHHDRNVNVSINILIKGYV
                     YKHCLRKHQEP"
     gene            complement(13469..14908)
                     /locus_tag="IWO38_001955"
                     /pseudo
     CDS             complement(13469..14908)
                     /locus_tag="IWO38_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(15456..16127)
                     /locus_tag="IWO38_001956"
     CDS             complement(15456..16127)
                     /locus_tag="IWO38_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ0314040.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            complement(16476..17879)
                     /locus_tag="IWO38_001957"
     CDS             complement(16476..17879)
                     /locus_tag="IWO38_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0314041.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTISS
                     AESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFSTPQQIESTESFQSQPI
                     LSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGP
                     QMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGD"
     gene            complement(17991..19334)
                     /locus_tag="IWO38_001958"
     CDS             complement(17991..19334)
                     /locus_tag="IWO38_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0314042.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFSQ
                     EYDKLILATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPVFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            complement(19468..>20172)
                     /locus_tag="IWO38_001959"
     CDS             complement(19468..>20172)
                     /locus_tag="IWO38_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0314043.1"
                     /translation="DADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKV
                     DVSTAGNKKGIVVVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPN
                     AEVTVTLPNGSQYHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSN
                     EKDSEKAVSKDNKTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILL
                     GTLGLLGYKNRKKENE"
BASE COUNT         6506 a         3418 c         3751 g         6497 t
ORIGIN      
        1 aatggttttt aaccaatgct caatctttag tcttactagc gattgtggtc attggtttgt
       61 ttttaggatt taaacgggag ttttccaaac tgattggctt cttggtgatt gctttgattg
      121 cggtaggtct ggtctttaat gcttctggtg tgaaggatgt cctactcaat ttgtttaacc
      181 gaattgtcgg tgcctgaggg ggaatcacaa tggaacagat gcgtgtttat attgcgaacc
      241 ttggaaaata caatgaaggt gagttggtgg gggcatggtt taccccacct gtcgattttg
      301 atgaagtgaa agaacagatt ggattaaatg acgaatatga agagtacgcg attcatgatt
      361 atgaactgcc ttttgagatt gatgagtata cgccaattga agagattaat cggttgtgta
      421 acttagcgga ggaactagaa ggaacgccaa ttggtgaagc tgcttctgag attcaacacg
      481 ctttcttcaa ttctttcgag gaaatggtag agcatgtgga tgatattgtc tattatccag
      541 attgcgatga tatgagtgat gtggcgcatt acttgattgt agaagctggt gattttggcg
      601 aagttccaga acaattgaag ccgtatattg attttgatgc ttgcggtcga gatttggaga
      661 ttggaggaaa ttatttggtt actagtcgtg gagtttttga gtatccagaa tgaatcaaac
      721 actatatata gtgtttgatt agtatatgta agcaaaaaaa cacaatatat agagtgcaaa
      781 ctgaagggta atgtgatata atatttgccg aagaaatatt tttgaaatga ggtaatatta
      841 tggcaaatat tcaaacaagt tttattgatt tccataattc aattagattg gacgtagaag
      901 acaatacgct acttaaggac tataaagacc aagtaattga tggattaaaa gattacttac
      961 ctgatgatgt gaagtttgaa acttttttac agggaagcta ttctgtttat accggaataa
     1021 aaagctgtga tgaaaaaatt gattttgaca tcgatattgc agttgctttc gaaattgatc
     1081 atactgttta tgaagatcca agggagccta aactgtgggt aaaagaggcg ctagttgaaa
     1141 tttttcctaa tgctcaagtt aatttaaaag taccttgtgt aactgctaca tttactggaa
     1201 aaaagacaaa aaaaaatgtg cacgttgatg ttgcggttta tgctaaagaa gatgaaaatt
     1261 atttcttagc aaaagcaaaa gaattcagtg ctcccgaaaa ccgatgctgg gaggaagcgg
     1321 atcccaaagt attaaaagag aaaattaata gtcatgtagc agattcagat gatcgaaaac
     1381 aatttcgaag atgtattcgc tatttaaaaa gatggaaaga taacaatttt aaccaagaat
     1441 ataaaccaac aggaattgga cttacaatta atgtgatgga tacgttccta gttaataagt
     1501 ccactgattt tttaactaga aaagttcagt ataatgatat ggaatgtatg aagcagattg
     1561 tttcttcgct gaaagattca tttgtgtatg agtacagtga aacagatggt tggcattatc
     1621 gtctacatgc atatcttcca gtgaagccta acagtgacac ctattcaaaa atgacagtga
     1681 atcagatgag tgattttaaa aacaaacttt ctaaattata tgatgatttg attttcgcta
     1741 tcgatactga agatgagtat gaagctacaa aacgattaaa taatcaattt ggggaggatt
     1801 ttttaattat ttcagaagaa gaagttacag aaaaaaactt aagaaatgca tttgttacag
     1861 actatccgag tgcttaggtt ggtgttaaag attggattat actgatattt taaaagaaaa
     1921 gctaacagat gttgtgcgtt tattcgatgg taattactat ggggattttt catttgctac
     1981 aattggaaag ttgcgtttaa aaattgaaat accaaaacat tttccattgg tacttcctag
     2041 gatttacatt cataattttg atcatttagg actttaccta ccacatgtag aaaaagatgg
     2101 gttggtatgt ttttcttcag atataaatta tttagtagat gaagaaaatt cagaattggt
     2161 tttattgcag tgtgttaata aaactataaa aacaattgag gaaggtttga aagggttaaa
     2221 taaaaatgat tttcgaaaag agtttgttgc tttttgggac aaacaagaga atcaaatttc
     2281 gttattctac ctttgtgaac cagcgttgtt tatcaagtca gttgatacaa taatgtacgg
     2341 taagtctttg gttgctttgg ataaagagaa cgaattacaa ttaaatgcag cgagtagatt
     2401 gtttaatgga gagaaagaag atactcatga attagatgca tactatgttc caattagaaa
     2461 caagaataat attatgcccc cgaagaataa taaagcatgg tcaaagaagg atattttaca
     2521 aatagtaaaa aaaaatgtaa caggtgcaaa taaaagattt tttttaaaac ttgaaaaaag
     2581 aaagaataga cataatgttt tacttattct agatatccca attgaagaaa agtcaaataa
     2641 tgtcataatt ggatttcttt ttacaaaaca atctggatct ataaaagatt ataagggtac
     2701 gcctaggact gtattagttc agcgtcatga tgaattatat ttggtgaata gaacgagcgg
     2761 agaaaactat ggattgggtg ctaatatagc tattgttggg gtagggtcag ttggcagtaa
     2821 aattgctgaa aatcttggtc cattaggtgt gagaaagatg acactcatag acaatgattg
     2881 ttttcaagct gaaaatttgt atcgaaattc acttggtgca gatagtttaa aaaatgcccg
     2941 atctcagaat aattttttta aagttgattt aatgcaggag gagctcgaaa aaaaattccc
     3001 atacctttgt gttgaagtgg agtcaatgaa cgttttagac ttaattagta tgcaacctgg
     3061 ttttttcaag gaatatgact atgtttttgt ttgcatagga gatacaatgg caagcctggc
     3121 tttaaataat tatttttcca aaagtagtca aaaagttttc tattcttggc tggagccata
     3181 tggcattggg ggacacactc tatttataaa ttattctcta aatcaaaaag gttgtttcaa
     3241 ttgtctttat acagattcaa actctaaaaa taggtgttca aatagagctt ctctggtaaa
     3301 agaaggacaa atttttgaaa ggactttagc gagttgccgc tcaaaattta taccatataa
     3361 tgctattgcc tcgacggaat gtgcaattaa tgcaataaaa cttttccata aagtcaatca
     3421 aaataatatg accaatagta ctcttttttc atggatagga gatgggagta cttttgagaa
     3481 tttaggatat caatatagcg acagaaatac ttttcttaat ggagtcaata agtttgaaga
     3541 aagattcttt tctaaatatt gcaaagtatg tggggagtta gatgattaaa ttttcgtgtg
     3601 accgtattgt cattgaagta agggatgaag ttcttaagat atttgaacaa tatcggcagg
     3661 gaaaggatga cttgcctgaa gctggaggag ttcttcatgg aaaaagactt aagaatggga
     3721 gcataataat taccgatata acaacacctc aaataggaga tatacaaaaa agattactct
     3781 ttaaaaaaaa ccgagaaatt catcaacagc tttctaatga gaaatggata gaatcaaata
     3841 agttttctat tgcattggga gaatggcata ctcatcctga agctgtacca actccatcaa
     3901 atgttgataa gaaaagctgg agactgaacg tgagcaagca acatgatgat agagtttatg
     3961 tttttattat cgttggattt tctgatttga gaatgtggtt actgtcaaaa aaatcagata
     4021 ttaggcaggc tataaaacta taaaggagtg attaggtgaa atcacaaaag aatatttggt
     4081 ggtgggggtt tttagtagga aaggtaatga taccagccct tctcttaatt tctctaccga
     4141 tgattacttt tttctttacc aataaaaaaa tgagtaaaga agccgtgatt tttttctttg
     4201 gagaccaaaa agctaccttt attgaaacgt tagtaacatc tcttcagctt aatttgaatt
     4261 acattttttc aattattatt gttatatcgt tattgaattt ttttaaaaaa agaaattcag
     4321 gaaaggtgtt taactctaat ggaaatgtgt actacaacta tttttatttt gtattttggg
     4381 tagcagcaac tttacttggt tatgataaaa ttcagattgc aggaattcct atacacatgc
     4441 aatataagtt ggttttgagt ggaatttttt ctgaggtgtt gccagatata tacgatgatc
     4501 attacgacag tgatggaaca tgtaaagtaa gcattgaaaa agaaaatttc gatgacatcg
     4561 atggatatga ctctgtgaac ttactgatta ttgatactta cgatataaaa atgagtgaat
     4621 tatctatgga aaatcaaaca tatcctacga taattgttag agggaattca atagatggag
     4681 ttcgtaaagt taatagatct ttaatcttgg aaataaaaaa aacaatggat gaaattcaaa
     4741 aaagtgactt taaaaaagta tttgttgctt caacatctaa tccaaaaaat agtatcaata
     4801 ttattaattc atcgtttaga tttttcggtc gatcacgtag gtttaaactt tatgttcttc
     4861 aaaaggatta tgcaagcaat ggaaagtata gtaagaaata tagaattttt atttaagaag
     4921 gaatttttcc ttctttttat tttggaggaa actatgaaaa aaataaaaag ctacaccagt
     4981 atttgggcag ttgaaaaagt catttatgcg attaatgact ttcagctgcc ttttcctgtg
     5041 acctttaacc agatggcttg gtttgtttta tccttgatgt ttgtcattgt atttgcccat
     5101 gtgcctccgc tatccatgat agagggggct tttttaaaat acttgggcat tccgatagcg
     5161 gtcacttggt tcatgtcgca aaagaccttt gacggtaaga aaccattggg atttttacga
     5221 tcctttatca gctatcactt acggtttaag gtcacttttg caggaaagaa agttaaagaa
     5281 cagaagaaac gatgggacga accaatcacc ttggtaagga gtgtgaatta tgtacccgat
     5341 taagtatatt gaaaataatc ttgtctttaa ccaagaaggc gagtgttttg cttactatga
     5401 attagtgcct tacaattact cctttttgtc acctgaacag aagtatcaag tacatgataa
     5461 ctttcgccaa ttaattgcgc aaaatcgtga aggtaagatc catgccttac aaattgccac
     5521 agaaagtagt attcgtgcca cacaagaacg ttcgaaaaag gaaattaccg gtagactcaa
     5581 agaagtagca aagcaacgaa ttgatttaca gacagatgca ttagtatcca tgattggcga
     5641 tagtcagatt gattatcgct tctttattgg ctttaaactg atttcgacag atgaagaggt
     5701 caatctaaag aatctcagga aatctttctt ttctggactt caagaatttg tctatggagt
     5761 gaatcatcat ttgatgggtg attttgtttc cttatccaat gaagaaattc gtcgctatac
     5821 caagcttgaa aaactaatgg aaagtaaatt ggctcgaaga tttaaagtca aacgagtaac
     5881 gccgagtgac ttgacgtatt taattgaaca tatctatggt gaaaaaggca caccgtttga
     5941 agaatatgaa ttccagctac caaagaaaaa gctgaaatca gaaacgctgg tgaaacgtta
     6001 cgacttatta cgtcccactc gttgtttgat tgaagaaaag cctcggtact tacggatgga
     6061 acatgaaaat catgagtctt atgtagcgta cttaacaatt aataccattg taggggaaat
     6121 ggagtttcct tcctcggaac ttttctatta ccagcaacaa caatttacgt ttccgattga
     6181 tacttcaatg aatgtggaaa ttgtgacgaa taaaaaggcc ctagctaccg ttcgtaataa
     6241 gaaaaaggaa ttaaaagatt tagacaacca tgcctatcaa tctgataatg aaacgaactc
     6301 caatgtatta gatgctttgg attccgtgga tgaattggaa accaccttgg atcagtcgaa
     6361 agaatccatg tataaactaa gctatgtggt tcgagtgagt gctaaatcag tggatgaatt
     6421 aaaacgccgc tgtgatgaag tgctcgattt ttatgacgat accaatgtaa aactagtccg
     6481 gccctttggc gatatgatgg gtctacacaa agaattctta ccctcaagta agcgatacat
     6541 gaatgactat attcaatatg ttacgtctga ctttttggcg ggacttggtt ttggggctac
     6601 tcaaatgcta ggagaattag aaggcattta ctttggctat aacgtagata cggggcgcaa
     6661 tgtttacctg aaaccggcat tggcatcaca aggggtcaaa ggctcagtaa ccaatgcttt
     6721 ggcatctgct ttccttggtt cgttaggtgg ggggaaatct tttagcaaca atctcttagt
     6781 ctattatgcc gtcctttttg gtgggcaagc tgtgattgtc gatccaaaag gagaaagagg
     6841 cggttggaaa gaaacgttgc cagaaattgc ggatgaaatt aatattctta acttgaccag
     6901 taagccagaa aatcaaggct tacttgatcc ctatgtgatt atgaagaaaa agaaagactc
     6961 agaaagtttg gcgattgata tcctgacctt tttaacaggg atttccagtc gggatgggga
     7021 gaagttccct gtgttacgca aagccattcg aacggttacc caaagtgatc agcgaggatt
     7081 attacaagta atcgttgaat tgcgtaaaga ggactcacca gtcgcggaaa atatcgcgga
     7141 tcacatcgag tcctttaccg attatgattt tgcccatttg cttttctcag atggttcgat
     7201 tacccagtcg attagtttag ataagcagtt aaatatcatt caagtagcgg atttggtttt
     7261 accagatgca gaaaccactt ttgaagaata taccaccatg gaattgctga gtgtttccat
     7321 gctaattgtt atttccactt ttgccttgga ctttatccat agcaaccgag aaattttcaa
     7381 gattgtggac ctggatgagg cttggagttt tcttcaagta gctcaaggaa aaacgttatc
     7441 gaataaatta gtgcgtgctg gtcgggccat gaatgccggt gtttacttcg tcacgcaaaa
     7501 cagtgatgac ttgttggatg aacgtttgaa gaataatatt gggctaaagt ttgcatttcg
     7561 cagtaccgat attcatgaaa tcaagaaaac cttggaattc tttggattag ataaagaaga
     7621 tgaaagtaat caaaaatgca tgcgagattt ggaaaatggg caatgcttga tgcaggattt
     7681 gtatggtcga gtaggtgtgg tccaagttca tccagtcttt gaagaacttt tccatgcttt
     7741 tgatacccgt ccgccagttc aagagagaat ggagtgagga gatgaaaaag aaactttttc
     7801 gtatctttgg aattgtttta atcagtttgg tcactctgat aatacttttg agcttagttg
     7861 gaacggtagc tgaggcaaca ggtctggttg atgatacagt tggagccgga aatctttatt
     7921 cccaatattc acttggtaac tatcagttgg atttctttgt ggatagttcg tgggattggc
     7981 tgccttggaa ttggggtgat ggcctaggga agagcgtaat gtatggactt tatgccatta
     8041 ctaattttat ttggacagtt agtctctact tatcaaatgc cacgggctac gtggttcaag
     8101 aagcctataa gctggatttc atttcagata cggctgaaag tattgggaag aatatccaaa
     8161 ccttagccgg cattaccgaa aatggactcc agacctcggg cttttacttt gggtttctat
     8221 tattgatgat tttagctcta ggagtttatg tggcgtatac aggattatta aaacgagaaa
     8281 ctaccaaagc agtccgggct gtcattaatt ttgtgatgat ttttctactt tcgggttcgt
     8341 tcattgcgta tgcgcccacc tatatcacaa aaatcaatga ttttagctca gatgtcagcg
     8401 aagcggctct tagccttgga accgagattg tcgtgccgaa ttcagaaagc caaggaaagg
     8461 acagtgtgga tttaatccga gatagcttgt tttccattca agtccaacaa ccgtggttac
     8521 tcttgcaatt tgatgattcc aatatagaag aagttggcga agatcgtgtt aacaaaatcc
     8581 tgtctgtaag tccagatgaa aataaaggca aggatcggga agaagcggtc aaagcggaga
     8641 ttgaagataa tgacaatgcg aatctcagta ttaccaagac catgaaccgc ttagggaccg
     8701 tggtcttttt ggtgttgttc aatattggca tatccttctt tgtctttctc ctaacaggga
     8761 ttatgttgtt ctcgcaaatt ctctttatta tctttgcgat gtttttgccg attagtttct
     8821 tattgtccat gttgccgact tatgagagtt tgggcaagaa ggcgattatt cgcttgttca
     8881 atacgattat gatgcgagca ggagttactt tggtgattac gactgcattt agcatctcca
     8941 cgatgttttt caatatttcg gcaacctatc catttttcat ggttgctttt ctgcaaattg
     9001 tgacctttgc cggtatctat ttcaaattgg gcgacattat gaatatgttc aatctccaga
     9061 gtaatgatag ccaatcaatg ggaagacggg tcatgcgaaa accgcaaatg ttaatgaatc
     9121 gaacattgcg gcaactcaat cgaaatgtgg gacggacttt ggcttttggt ggtggtgcag
     9181 cagttggaaa taagttggca aaggaacaat caaaatccaa atttaagcca tccggttctt
     9241 ctctacgaaa gaattcacga ttaccgaacg atcacgagat accatctgac ttgaataagg
     9301 aaaagccaat tccaaataat aagaaacaat cccgaatgaa tttggccggg agaaaagtcg
     9361 gcaaggttct ggatacccaa gctttggtga aagataagac caaacaagta aaggatggag
     9421 tacggaatac gccaaccaac ttgaaataca acttccataa aggaattgaa aaaacgaaaa
     9481 aagcaccgga agagtttaag cgaggactgg tccaagaaaa agcaaatcga ggagagttgc
     9541 gagaaaaaca acaacaacgt agagacgaaa aaatagctga aaagcgcaaa gtattgaacg
     9601 aaatagggaa tcctcatggg aaaagaaggg aaaatgttcc gataaaagca aaggtccaat
     9661 ctcaaaagga tagagcacca aagcggattg tgagagcaaa ttcgaatccc gaaatcaaac
     9721 gaaaattcgc tagccaagaa atcatttcaa aaggaataaa ccaatcctca agtaagaaaa
     9781 attcttttca acaagtgaaa cggcggtcta ctctgtccaa acgaaccaat cgaaaagtac
     9841 aaaaaagttt gaaccctcat cccaaaccaa ggtcaggtga gaaaaaatga acgtaaagaa
     9901 attaagtgcc atttcgttaa ctttcctaac aatctctttt atgggtatct tgatgtgtgt
     9961 gggcttattg tttggtgaag acagtgaagg aagtagtagt tcaggtgctc ctcctggggg
    10021 gagtagtgtc tcagaagagg ttttgaaaca ccgtgcaatg gtggaaaaat atgccaaaga
    10081 atcggatata acagagtatg ttccgattct tttagcgatt attcaagtgg aatctggtgg
    10141 cacaatggaa gatgtgatgc aaagttctga gtcagccggt ttaccaccga attcattaag
    10201 taccgaggca tccatcaaac aaggatgtct ctattttgcg tctttggtta agcgttccaa
    10261 agaattaggt tgtgatcaag atagtatcat tcaggcctat aactttggcg gtggttattt
    10321 ggactatgtg gcaaaaaacg ggaagaagca tagttttgct ttagctgaat ctttttcaaa
    10381 ggaaaaatct tgcggtcaaa aaatcgatta ccctaatccc atcgcgaata aggaaaatgg
    10441 tggatggcgc tataactatg gcaatatgtt ttattgctta ttggtgaagc aatacctaac
    10501 tacaacacag tttgacgata aaacagtcca agggattttt gacgaggcgt ttaagtatga
    10561 aggaacggct tatgtatttg gtgggtcaag acccgaaaca ggatttgatt gtagtggctt
    10621 gactcaatgg tgttatgcca aagttggtct gaaacttcct cgggtggctc aagatcagta
    10681 tgatgccatg acccatattg acctaaaaga tgccaaacca ggggatttgg ttttctttca
    10741 ttcgacttat gatgccggta cttatgtgac ccatgtcggt atttatgcag gggagaatcg
    10801 catgtttcat gcaggagatc cagtaggctg gacgaatcta acagaaagct attggcagca
    10861 gcatcttatc ggtgctggac ggtacaaata agagaaaaga ggaaaaagaa tgaagataaa
    10921 aattgaacgg aatcaaaagg aaaagagcct aaaaaagaaa aaagaacgag tcgtatccgt
    10981 gggtaagcat cgaaaaatgg tgcttgccct ttggctttta ttatcttgta gtcttacttt
    11041 tggcgtttat aaaaatttca ccgccattga tcaacataca actcatgaaa aagtggttat
    11101 taaagagaaa gtagtgaata cttctggtat tgaaaacttt acgaaggatt tttcgaaaga
    11161 atatttttcg tggaaaaata acaaagaagc aattgaaaag agaatgaaca acctcgaaca
    11221 gtaccttaca gaagaaggtt tcgctttaag tcaagatatg gttcgagcag atattcccac
    11281 taattccgaa gttcagtcag taaaagtctt agatgttgaa aagagctcgg aggaatttgt
    11341 cgtttcattt ttagtggagc aaaagattac agagggaaaa aaggcacaat cgatttcttc
    11401 tgcataccga gtgacgatct ttgaagatga aaacaggaat tatatagtaa cgagtttgcc
    11461 aactatgatt gcaaaacctg atagggcaaa atacaagaca aaacaagtag aaaatgattc
    11521 gaaaattgat gccaaaacta cagaagaaat cgctgagttt ttggaaacgt tctttaaatt
    11581 atatccatca gcttctgaaa aagaattgga gtattatgtt gaaaatgatg cgatgcgacc
    11641 aatcaatatt gacttaaagt tttcgaagtt agaggatcca gtttacctac aaaacggaaa
    11701 aaaaattcta gtgagtacaa agataaaata ccttgataat gtatcaaaaa ccactagcta
    11761 ctttcagtac aaattaatat tacaaaaaaa tggaaactgg aaaatcattg atgttaaata
    11821 ataatgactc aatctaaatt tatgagaagt tataagtagt ttaacttatt tcaggcagaa
    11881 gtcgcccatc ctctataggt gggtgatgaa tgccgttcgg taactcgaaa accgacaatt
    11941 aagacatagt gcctttttat tttaatccat gtcataatca gtctatataa gaaatggttg
    12001 atataccatg gaattagata caaataatca ttcattattt cttctttgtt accaccttat
    12061 tttagtagtg aaacattgta aacatctcat tgatgatgta atgttagctt atgcaaaaaa
    12121 ctatctttga aagaatatca gaatcacatc acattacctt agttgagtga aatcgtgata
    12181 aagactactt tcatattatg ctcaaagcac aacctaaaac cgaattaaca aaattcagca
    12241 atgcctaagc tcatgtctga taaaatgaga ctttctaagg gtcaaaaatt tcctttgaat
    12301 acgatcatta gcttagataa aagtaaaaac cttagaacta cggggatagc ttggttaata
    12361 agagatatcg ctgttaataa agacataagc ttgcaagtat gctctattcc caagaatctc
    12421 ccacttcaag cactaaggat cgttaaattc agctaagtgg caagtagttc actagacgat
    12481 aattatctcc atatttcaat ctcagctttt ccatttttca tagcaaattc cgttattttt
    12541 atagcatcta aaagattcag cttttccact gatctttcac cacttttcag cttttttatt
    12601 gtgcttaaat tgataccagt accttttgat attgaattgg aagtaaaatc attaagggtt
    12661 ttttttatta aattcatatc cgcaattcca gcgttcataa gaatgaagat tctcctctca
    12721 tagactactt tgaaaatggt actacagtgg ctgtttatat atttcagtag gttactgtag
    12781 tggtcatttt attataatta gaaaaggaac ctaaatcaaa aatgctaaag aaaattttca
    12841 tagtattaaa cctatcatga ctaaagccac ggaattcttg ataaaagagt atacttgcaa
    12901 gcctatgtct ttactaacag cgatatctct tattaaccaa tctatccccg ttgttcctaa
    12961 ggttcttgat gttttctaag gcaatgcttg tatacatagc ccttgatcaa aatattgata
    13021 ctaacgttga cgtttcgatc atgatgtgta cggcaaatag gacaagtcca ttctcgaatt
    13081 gtgaatgact tcttaccatc tgtgtgtcca cattctgaac caatttgact agacggaaat
    13141 catttatcta tcttgatgat ttttttgaca gaagtatcga aaatctttac ataatctggg
    13201 tgttgcattc ttatgcagat gagagcgtta gctccgtatt cataaccagt tgcctctaaa
    13261 acgtagtcca tcggtggata cctagcttgc ggttgccagt ggctttcgca gtcttcttgt
    13321 aatcaaatcg agcatctaat tgagtactag tattttgaag ataatacttg agtttttcgg
    13381 ttttcagaag ttcacttcat cttccatcat ccatggaaat cccactcacc catacgcagg
    13441 cctccaaaaa ttcttcaaca atggtttttc agacattttt ctgaatacct ccttgttttt
    13501 tgatacgcca gatagtcagg aaaaaggcaa caaaacacaa aacaaagctg cctaaaaagg
    13561 taatcttcat tccatataaa aagacatccg gccgatctgc gatatagtta gttactcgtt
    13621 ggcccatctt agcagacatc gcttgatata agaccgtcgt agaaagcgaa atcccgatga
    13681 ccatccccaa atttcgggca aaggaattca tggagcctgc cagtcccaaa ttttcgatag
    13741 tgacagaact cataaccatc gtattatttg gggactggaa caaggcattg ccggcaccca
    13801 tgatggcagt agcgacaatg tacagccaca aaggagagtc cactgacaaa aacatataca
    13861 tgacttgggt taccgacaat aaacccaagc ctactaatac aaaaatctcc gggccaaccc
    13921 ggtcggtcag gtatccgctt aaaggcgccc cgacgaccat catcaaggga aagaccatca
    13981 tcaacagtcc ggcatagcta ggactcaacc cgcgagcatt ttgcagataa aaaggaatca
    14041 ctacattaac aaagaagttg gaagaaaaaa tcaacaaggc tgtaatcaag ctcatggtaa
    14101 aaatcttgtt cttaaaaata ccgaaagaaa tcaatggaga ggctgctttc ttttcaactt
    14161 ttaaaaagct gacaaaggca atcactgaaa ttacgaacag tacaaggggg actggatgga
    14221 aaaagccact ttcttgcccc aaaaagattg ttccaaaaag gctgataatc gtagccgcaa
    14281 tggcagcaaa gccccctcca tcgattgtat ggccgctcat ggtgatatct tttggtaaga
    14341 atttttcagc aatcaacatc gttacgattc ccaccggtac attgatccaa aaaatgtacg
    14401 cccaagaaaa ttgggacaaa atcaagccgc caagacctgg tccagcaatc gagcctaatg
    14461 aaacaagcgc accgattgct cccaatgccc gaccacgttc atgtatggga aagacttccg
    14521 tgatgattcc ggcatttgta gccatagtca tactagcacc aatggcttgt accacccgtg
    14581 cgaacaatta aatacctagc gaatgattga acccacacaa cagcgagccc aagacaaaga
    14641 tcagcgtacc aatccgaaat actttgattt tcccacagct atcgccaatt ttaccaaaca
    14701 gcaacaaaca agaacagaca atcatcagat aaacggacac tacccattcc gcttgattca
    14761 ttggtatgcc catatcatct gagatggtag gtagtgcaat attgacaata ctgccatcca
    14821 atgtggacat gaaggtaaac atagatatag caatcaaaat ccaccagcga tttttttgga
    14881 cttttgcatc ttcttgataa ctatgcataa taggatttcc tttctaatga tcgtgtagct
    14941 aagaacaata taacctatgt ctactgccca acaacttctc gcgactctag ccataggaag
    15001 cttgagtaat ttcgatttgt tattctcatg taaccatgta agtttgagat gctttatttg
    15061 aacttactct atagtaacta gtgataattc gctttacttt cttctagcaa tttctaaaag
    15121 tcgacttcat tgttcttcag attaatagtg tgagctatct cgtgagacgt aagaaaattt
    15181 ccattctact ttttcccaat tttttcatat ttatggtgtt gcacctattt gttttccaat
    15241 ttgcttttca aatacattgc tatgatattc aattccatag gattatacta aatgtcaata
    15301 gtatgaataa tatattatag atgtttatat atcactgtta attattatcc acaataatta
    15361 atgattacct cgatatttgt caatcaaagg ttgaaacaat ttattttcag catcttttct
    15421 aacttgagct gcactcttaa tagaatcata cgatcctaaa tagtacctct tttttttaaa
    15481 gttatatacg caatccattt ctctttagaa cgataaactc ccttaacacc acttgtatta
    15541 tttttcggta attttgacgt tagacactct attactgtcc cttcaacaca tgtccttgtt
    15601 tcttgccttt ttaaacatcc acaactttga gtatgtccaa aaaataagtt actataactt
    15661 atctcacaca tactgccaca ctcacattga caaacccatt ttgttttcca tgaacagtta
    15721 ttgtccaaag gatactttgc agtaagtaat ccgaattttt tattttcaat gtgatgcttt
    15781 tttgatttca aacatccaca gctttttaca tcacctttta gaagtcgctt tgccgatact
    15841 aacttttttc tcccacacag acattgacat tcccaaacaa cagttccttc acttcgcagt
    15901 ttagttggct tgattgcaac taacttacca aaagttttat ttgtcaaatt tttatattta
    15961 tgcctatttt tataatttcc acatccacaa cttttggttc tacctgatag taagtttgat
    16021 cgatatacaa tcgtttcatt cccacagtca cataggtaac gatatttttt atatccatta
    16081 tgagtaatct cctcaacaac agtaaggtga ccatatcttt ccttcactgc acctaccctc
    16141 ccaagaaaga agtaccatat aatttcaatt aacttttaat aaatcagaaa aaaatatttt
    16201 caatttttct tagtctgatt ttatgactaa tttataatac aagactgaac gatacgtaca
    16261 cgaaatcctc aagatagata aattgttttc atctagtaaa acctgctcaa aatatgagca
    16321 taaagacggc aataatacac tcgatattcg ataatggact tgttttattt gtcaaactca
    16381 tcgtaatcga gatatcaatt ccagtctcaa tatcctgatc aaaggtctaa gaatccaacc
    16441 attttcttgt tactttgtca gattgattac cttttttagt ctccgaataa taaggttttc
    16501 ggatcaacta acatgctctg agaaagacta ctatctttgc aaatttccaa atgaaggtgt
    16561 gggccagtac tattcccagt acttcctaca tagccaatta tttgtccttg caaaacatta
    16621 tctccagttt ttactaggta ttgttgctgg tgagcatata acgcggttga tccatcttcg
    16681 tgttgtatca ccacgtaatt tccccaagag tagtgaaact ctgctttaac aaccttacca
    16741 gctttaactg catgaatcgg ctcaccctgt gtagctgcca tatcaagtcc tcggtgaaat
    16801 tcatttccac gatttccaaa caaactggtt attgaataat tatgaatagg cacttggtat
    16861 ccagtcttac tcatttgtgg acctttaact tctttatcat agttggttaa atcataggtt
    16921 ttaattaatt catttagctt caaatagtat tgtgtatctg ttgcataccg tcctgtaagg
    16981 aatttagttg cttctcgata tgttttacta ttagctttta atgctcctgc ataaaaatct
    17041 ttatttccac taattccctt agttaataat ttggcataat cttcaaaact ttcctcatag
    17101 ttttcataca ctctaaattt tgattgggtc gtataaagcg ttccattacc caaatcttcg
    17161 tttgttacca taatgacaaa gtttccatta taagttcctt ttatcccaaa taaattgtaa
    17221 ttcggtgcct gagctaactg gctttgacca ctggcagatt ctaagatcgc ttgtgcaatc
    17281 ataacagaag catataaatc atatttctga ccaatttttc tcgcagactc accaattttc
    17341 ataataaaat tttctacaga ttcatttttg tcaaaatgaa tttcttcatt accagatagt
    17401 attggttgtg attggaaaga ttctgttgat tctatttgct gtggtgtcga aaaatttttt
    17461 gtttgtcctt ccgtttttgg cgcttccttt tttgaagact ctatatggtt tggtcttgtc
    17521 actttcgaag aagttatttc acttgtacta cttgaagttg ttgaagattc ggcacttgaa
    17581 atcgtactac tttcacttga cgaagttgtt gaagactcgg catttgaagt cgtactactt
    17641 tcacttgatg aagttgttga agactcagca tttgaagtcg tactactttc acttgataaa
    17701 gttgtcaaaa gttcactact ctctgctgtt acatcggaat tactcatttc agtcgtctcg
    17761 gttctttctt ttgcaatttt gtatgttgaa ttttcgcttg ccaacacatg gaaaggtgtt
    17821 gctataaaga gttgggacat taatataact gctgatgcaa gttttaaaga ttttttcatt
    17881 tgatttacct ctcttttttc tctttaatag tgttttctat taaaaattat gataaatatt
    17941 ttcaatgaat tgagtaatac agtatatttc ttgatttcat ttagtaacga ttatgtttca
    18001 atgtttaatc ctgttactgc ctttaaaata aaattatagg gttggctaaa actaggatga
    18061 aataaataat ctaacatttt caatttgtct aatgtaattt gttcttctat agcaagagaa
    18121 aacattgata atagtccaga taaatcttct tgactacaaa gttgtacacc tactattctc
    18181 tttgtatttt tttcaaagct aattcttaat tttacacgac tattttttct caaaaaaaca
    18241 ggtctaatga aatcttctat ttctatataa tctatagact cacttaataa tcgtgctgtt
    18301 tttaaattca aacctgctgc gtaaaaattg taattaaata tagaaaagcc atttgtaaat
    18361 tgggaaccag aaggtgtaaa atcttgattg tcaatatgtt gagttgcaat ataagctgtt
    18421 cttagagcat tagaaataga aaaatcaaca ctacttctat taatggacgt aaaaaaagtt
    18481 gtagcacaat ccccaatagc atatatatct tttatatttg tttgctggta ttcattagtt
    18541 agatatgctc cactaacatc tctcaagagt tcctttttgc caattggact attagataga
    18601 aatcctatag cttgtacgac catatccata gagtattggg taccacttgt agtgactatt
    18661 ttttcaatct tttttcttcc taaatatttt ttaggacttt ctgatagaca aagatgtatt
    18721 ccattttttg atagtatttc ttcaacttta tctgaaaaac tacggtcgta ataagtcgac
    18781 aacactctat ctgcaacatc gaaaagatat acttctttcc ctctttttct tatggcttca
    18841 gcaatttcaa ctccaatata tcctgctcca ataattccta cttttttaat agtattatca
    18901 tctaaagctt taaaaatctc tttagcattt ggaaaattct ttatcgaaaa aatattagct
    18961 aaattattcc ccggtacatc taacataatt tgctttgaac ctgtagctag tataagtttg
    19021 tcatattctt gggaaaatct tgtgccatct ttttttacac aaataatctt tttattttga
    19081 atatcaattt tgtcaactgc tgtctccaaa aacaactttt ttatcttaga atttagttct
    19141 cttctatttt gataaaaaag atcttctaaa ttgctaactt ctctttttat gaacaacgat
    19201 gtcccacatt ctaagtaact aatttctgat tttttatcta tcagaataac ttcataattt
    19261 gaatcacata agtttagaaa tcgagctgca gctagccccg aatggtttga tccaataatt
    19321 actatttttt tcacttaatc acattctttt cccttctatg aaaagtatct tcatttttaa
    19381 ctaacaacct atctcccaaa ataaaaaaca aaaagaggaa gaaaaagatt cttcctcttt
    19441 tcagagaaga tttaagaaat tgattttcta ttcattctct ttttttcgat ttttataccc
    19501 taataaccct aaagttccta acaggattaa accaccacta atagtaaaga tattactatc
    19561 aatctctccc gtcttaggat aagcttttga ggactgcttt tgaggactgc ttttgcctct
    19621 atttttgttt tgcttgctac cttgattgtc agtcttatta tctttactaa cggctttttc
    19681 actgtctttt tcgtttgaag aaacaactac attagtaggt tcactagtat taccagattc
    19741 atcagttgca gttactatca cttttgttcc tgcctctaat ttaggaactt taactgtaaa
    19801 attaccattt ttatcagctg tcccatggta ttgactcccg tttggtaatg taactgtcac
    19861 ctctgcattt ggttcagttt taccagtaac aatttggtca ccctctgtaa taggatcaac
    19921 ttgaggttta ttcggtgctt ttttatcttc tacagaaata gtaacctcta cttcttcttt
    19981 agatccatct ggatatgtta ctactacaat cccttttttg tttccagctg tactaacatc
    20041 tactttctct ttccaagtat aagtagttcc ttttggtaga tcttctttgt tcttaattcc
    20101 ctcagatgcg tctggctctt tatttaagtc tgtagtcact ttttgtcctt caggattgta
    20161 tttatcggca tc
//
LOCUS       DACECY010000050        20112 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.054, whole genome shotgun sequence.
ACCESSION   DACECY010000050 DACECY010000000
VERSION     DACECY010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.054"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            4..2376
                     /locus_tag="IWO38_001960"
     CDS             4..2376
                     /locus_tag="IWO38_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314044.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="IWO38_001961"
     CDS             2437..3897
                     /locus_tag="IWO38_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ0314045.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="IWO38_001962"
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="IWO38_001962"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0314046.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESYRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYHKAAACTSEACDFRLWTTIAKKKLTATQLKEII
                     QNGRTSQPVKGLKGQKGSFEATIVLKEDFTTSFEFSEKKKTNYKKRTRRTTK"
     gene            6358..6954
                     /locus_tag="IWO38_001963"
     CDS             6358..6954
                     /locus_tag="IWO38_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0314047.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYSKAEWYELGKIEIKSRYGNPVIAYDPERTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTIRSYIMALN"
     gene            6967..7866
                     /locus_tag="IWO38_001964"
     CDS             6967..7866
                     /locus_tag="IWO38_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ0314048.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFENRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYEETMDSISNLMEKVI
                     HVEEKERKIAYQRHQQMNREMER"
     gene            7869..8354
                     /gene="ssb"
                     /locus_tag="IWO38_001965"
     CDS             7869..8354
                     /gene="ssb"
                     /locus_tag="IWO38_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0314049.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGILIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            8720..8980
                     /locus_tag="IWO38_001966"
     CDS             8720..8980
                     /locus_tag="IWO38_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314050.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9427..9633
                     /locus_tag="IWO38_001967"
     CDS             9427..9633
                     /locus_tag="IWO38_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ0314051.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9633..9857
                     /locus_tag="IWO38_001968"
     CDS             9633..9857
                     /locus_tag="IWO38_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314052.1"
                     /translation="MKTQHKLLNVKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFE
                     DLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            9872..10309
                     /locus_tag="IWO38_001969"
     CDS             9872..10309
                     /locus_tag="IWO38_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314053.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEAGVKPSTIKPSEYQALE
                     LTYGKFIETKKAKTVSSEWLDYFENVNKYGACYIMKKEDNENE"
     gene            10302..11009
                     /locus_tag="IWO38_001970"
     CDS             10302..11009
                     /locus_tag="IWO38_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314054.1"
                     /translation="MSNVVYQLTVEDVSHFLTNKEHLNEVVRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDCKRMPYQRFERKKGQ"
     gene            11390..11569
                     /locus_tag="IWO38_001971"
     CDS             11390..11569
                     /locus_tag="IWO38_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314055.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQELKQKYGKSESRIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            11662..11847
                     /locus_tag="IWO38_001972"
     CDS             11662..11847
                     /locus_tag="IWO38_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003027804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, HicA family"
                     /protein_id="HAQ0314056.1"
                     /translation="MPLTGKEMLKLLKKNGWVERRQEGSHHHLYKDGVRITVPVHANQ
                     DLGRGLEQKILKDAGLK"
     gene            11881..12312
                     /locus_tag="IWO38_001973"
     CDS             11881..12312
                     /locus_tag="IWO38_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HicB family protein"
                     /protein_id="HAQ0314057.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
     gene            13249..14523
                     /locus_tag="IWO38_001974"
     CDS             13249..14523
                     /locus_tag="IWO38_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0314058.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            14577..16004
                     /locus_tag="IWO38_001975"
     CDS             14577..16004
                     /locus_tag="IWO38_001975"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ0314059.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDHLYMKPVRELEQLRVG
                     SGVETKVNLAGEIIIAQDASFKEALIDVLLTKSKNQEIKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            16017..16889
                     /locus_tag="IWO38_001976"
     CDS             16017..16889
                     /locus_tag="IWO38_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0314060.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNELLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            16984..17979
                     /locus_tag="IWO38_001977"
     CDS             16984..17979
                     /locus_tag="IWO38_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0314061.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKDN"
     gene            complement(18584..18691)
                     /locus_tag="IWO38_001978"
                     /pseudo
     CDS             complement(18584..18691)
                     /locus_tag="IWO38_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            18807..>20112
                     /locus_tag="IWO38_001979"
     CDS             18807..>20112
                     /locus_tag="IWO38_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0314062.1"
                     /translation="MIIKNLGNVKSIDIHVKDLLIFTGKNNTGKTYTSYILYGILSEL
                     FGARFFNYFSHTEIEPHLQNSLNILLVSKDSIRKKFNNDAKKFLEENLKKIAVRNFKV
                     SENNFKNLSFTISDSEISELTGDIFNYEIKRYSISGIVITIQNVGEKLKISFEPTDSF
                     QYLSKDIEIEDIYKILAQELSKNVISLPKVFYFPAERTGINVFKNELNESRLKTYDTL
                     LNTVQFTNLTNQKKKAALKYDLLKKNIELLMETTDNSSYPKPISDYINYLNNMKNRYE
                     QKNVNNIASYIRDEIIMGKYEIDEKDNSVSFRQKFGKNRYRKERIPFHVVSSSIKSLY
                     GLDYYLENSSKEGDYLIIDEPELSLHPENQVKLAKVITDIVESKIKLITSTHSDLFVR
                     ELTNIVIENKLNSKNNKKNKLSDKNIGIYNFVGNNDINELEKI"
BASE COUNT         7344 a         3114 c         4014 g         5640 t
ORIGIN      
        1 gacatggcag aagaaaaaga accactagac aatagtcgct tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaga agatgtttga acaccagaga
      121 aagaccaacg gtcagccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gaacgtttag aaaacaaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcggc
      301 ctggatatga caatcgaaaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta
      421 gaacagctga aagaaagatc agaaaaagga aaagaaaatc ttcttcctga agtacaagca
      481 attattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 gactatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgcg
      661 ccaactgatt ctttgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt agaattgatc
      781 gatcaactca aaacgttgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgaa
      961 aaggaacaaa aacaaacggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taagcaagaa
     1081 gtcaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtgaaag aacttttgaa ttttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc tgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacggggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac ttcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagcg
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tgtagaaacg gcagagaata agaatattgt gcccgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtgat tttaaaagga
     2221 aaaagttatg gaaaagagat tcaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga aacagtgtca gaagtggcca aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgc gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt ttgaaaatga ccgaaaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagacta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac tctaagcaac aatttaaagc agccacagat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtagaaa atcttcattt tgagacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaaca tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaatg agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagttaaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac taaatcgtga tcagttaaac
     3181 gatacgtggt tagtttcgat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagttgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aagtcattta tgaagaagtc gcaaaagaag aaaagatcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaatt ggtttccgct
     3541 cacaaagtaa atgcgtcttt tggtcagcag ctttctgtta acgattcaat agaagctatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagcttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc tcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggattg gaatgatcaa ttgaagcatg aaatctacca acaagagatc
     3841 agaaagcaaa aacgtgccat gttgtttcaa caaggtagac agcaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaaaccgtca caagcgaagg cttatgccga
     3961 tagcttttct aaagctaccc gaaaagatgg gtattttgag atccaagatc gattgttttc
     4021 aggagaaact gtgatcacgt atggttttgg tcatttagtc gagttagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agagcatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatagcttg
     4261 gacgatcatt caacaagcag gcgccgatca tggaaagata tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagatg cagaagagac
     4381 ctacccaaaa tttgaagaag cacaagcacg ccagatcgcc gattggttga tcggcatgaa
     4441 cggtagcccg ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gtttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttacaggaaa agattagaaa
     4561 cttccaaaaa gaaccatatt ttgaaggaaa agcccaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggtacg atccaccaag ttgaaaccga
     4741 gaaaaaaagt gcggcaagtc cacgtctttt ttctctatcc agccttcaat ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagatac tttggaagcc atgcaagggt tgtatgaagg
     4861 aaaatatcta agctatccgc gaacggatac cccttatatt acggaagggg aatatgcgta
     4921 tctgttggat cacttagacg aatacaagca ttttttaaaa gccgaagcga ttccgactcc
     4981 tattcataca ccgaatagtc ggtatgtgaa taataagaaa gtccaggaac attacgcgat
     5041 cattcctaca aaaacggtta tgacagcagc ggcttttgaa caactaagtc cactgcagca
     5101 ggctatttat gaacaagtgt taaaaacaac ggtggccatg tttgcggaaa agtataccta
     5161 tgaagaaaca acgatcttga cgcaagttca gcagcttcag ctaaagacag tcggaaaagt
     5221 accagtagat ctaggttgga aaaagctgtt tggcaaagag agctacagaa aagaaaagga
     5281 agaagaaccg ctacttccta aagtgacaaa aggagaagcg gtgacggtag atctacaagt
     5341 ccttgaaaaa gaaacgaagc caccacaacc ttataccgaa ggtacgttga tcacagcgat
     5401 gaaaacagct ggaaaaaccg tggatagcga agaagcacaa tccatattga aagaagtaga
     5461 aggaatcggt acagaagcaa cacgagcaaa tattattgaa actttgaaac aaaaggaata
     5521 tatcaaggta gaaaaaaata agcttgtggt gacaaataaa gggattttac tttgccaagc
     5581 cgtggaaaaa gagccgctgc taacaagtgc cgaaatgacc gccaaatggg aaagttactt
     5641 attaaagatt ggtgagcaga aaggaacaca agctactttc ttagctaata tccaaaaatt
     5701 tgtctctcac ttgttggaag tcgtaccagg acaaatccaa tccactgatt ttggatcgac
     5761 gttacaagag gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt tagggatttg
     5821 tccaaaatgc caggaacaag aagttttact ctatcataag gcggccgcat gtacgtctga
     5881 agcttgtgat tttagattat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggaaatc attcaaaatg gccgaacaag ccagccagtg aaaggattaa aaggtcaaaa
     6001 gggttcgttt gaagcaacga ttgttctaaa agaagacttc acaacgagct ttgaattttc
     6061 tgaaaagaaa aaaacgaact ataagaaaag aacacgccgc actacaaaat aatggagggg
     6121 ttatcttatg cgaaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagcaaaaat
     6181 tcgttcagaa caagagaaat tacgtgccag taaaagaaga cttcagcaat taaagaacca
     6241 gcaacggatt caaaaagaaa ttgaattaga tcgataaaat cttcctggct attgtatacg
     6301 cataaactta tgttatacta tatttataag tttatacgta tacaaaggag ggagaaaatg
     6361 acaaaagcaa tggtttacaa gctgctagaa gaatttaacg gaactttggc tttgaaggat
     6421 gccaaaaaag cgggcatttc tcctgtaacg atcaaacgta tggtagatcg tggagagctg
     6481 gatcgtgagt atccaggttt tttcacctta cctggtcaat ttcctgatga attgtttatg
     6541 gcacaaaaga aatatgagcg cggcattatt tcgcatatca ccgcgttgga tttatatgat
     6601 ttaacggata tgatccctag acagatcgat ctaaccgtac cttacggtta tcacgtttct
     6661 gaaaaggggt tgaaagaatt cgcagttgag ttacactatt caaaagcgga atggtatgaa
     6721 ttaggtaaga tcgaaataaa gagtcgttat gggaatcctg ttatagctta tgatccagaa
     6781 agaaccttgt gtgacatttg gaatccctgg tacaacgttg aggatgaaat caaagtaaaa
     6841 gcaatcaaaa actacatgga gtcggacaga aaaaatctta gaaaattgaa tgagtatcga
     6901 cgaattttgc ctacagacaa aacgatacgc tcttatatta tggctttaaa ttaaaggaga
     6961 agacacatga cacctacgca attaaaagca aaagtgaaga atatttccag agaaaaagaa
     7021 gttgatccgc agcttgtttt aagacatttt atgatggaaa aattcttgga aaaaatctca
     7081 gagtctcctt atcgtgaaaa ttttgtgtta aaaggcggct tcttaattgg ctcaaagtat
     7141 ggcattgaaa atcgaacaac aaaagacatt gatacgactt tacgggaaat gaaagtaacc
     7201 aaagaaacat tgacaacggt tctaaacgat attttttcaa cgccgacgaa agaaggcatt
     7261 caatttgaga ttcaaggaat gaaagaaaca agggaagctg attattatcc agggtttagc
     7321 ttacgtgtac tggcacattt ggaaaacatg cggccagatt ttaaggtaga cgtgacgaca
     7381 ggtgactcga tctatcctgc aacgatcact cacagtcata aattgatgtt tgaaaatcga
     7441 acgattcctt tggaatctta tcctacagaa caaattattg ccgaaaaatt aagtgcaacc
     7501 tttgattttt taacagataa ttcgagagga aaagattttt atgatctcta tactattcct
     7561 aaaatggaaa agatcgatga gaagaccttg aaagatagtg tgagaaatac atttacacgt
     7621 cgaggaaaaa ctgatccgtt aaagatttat tacgaaaagg atatggatgt actgaaaaat
     7681 agtcatgagc taaaagatac ctggaagaaa tatcaaaaaa gcaatcctta tgctgcgtcg
     7741 atcacgtacg aagagacaat ggattcgatt agtaatttga tggaaaaagt gattcacgtc
     7801 gaagaaaaag agcgaaaaat agcttatcaa agacaccaac agatgaacag agaaatggag
     7861 agataaaaat gatcaataat gtaactttag taggacggtt aaccaaagat ccagatttac
     7921 gttataccgc aagtggcaca gcagtggcta cttttaccct agccgttaat cgtaatttca
     7981 cgaatcaaaa cggcaatcga gaagctgatt ttatcaattg cgtgatttgg cgcaaaccag
     8041 cagaaacgat ggcgacatta gctaaaaaag gcatcttgat cggcgttgtc ggacggattc
     8101 aaacacggac ttatgacaac cagcaaggac aacgtgtgta tgtgacagaa gttgtagctg
     8161 ataacttcca actattagaa agcaaagcag cgacagagag ccgtgcgcac gctgatcaat
     8221 cgtccacgag tccaagcacc accacttttg aacaacggga tacagcgacg cctaacaaca
     8281 atggattgaa cgcttcacag aatccatttg gtggacaatc aatcgatatt agcgatgatg
     8341 atctaccgtt ttaagtagca ttttctattg gttgcatttg ctacggttta tgttataatt
     8401 tagtcaacaa aaagaatttt caatctataa ccaaaaacca ctagctggcg ggctagtggt
     8461 tttcttttac gtttctatta taagaattaa cgagtaggct tactgtcttt cttggcaaat
     8521 ttacaatcaa gccatttgca gatatagtgc gcggctactt ctgccatgat tgcaaaaaca
     8581 aaatcaaaaa gaattttcaa acctataacc tcctcccctt gccagattag gccggggtag
     8641 cagacagcaa gactattata gcataattga aatatccaaa tgattggatt tttgctataa
     8701 ttttagcgag gtgattgtga tgaacaaata tgttggtttg cttgataaaa tccgagtaat
     8761 aaaaacgcaa ccgttattgg ttcgctttac tttacaaacg attcatgaat cgattaattg
     8821 tgtagttgca gatattgaaa ttatcgataa attgctaatt atggatgatg gaaaatacaa
     8881 tattgccgtc acaggtcact tcaataaacg aaatcagctg gttattgcta gcatgtatgt
     8941 tagaaatccc gatcatttta cacgatcgat gggcatataa ataaaagaag agttgtagtc
     9001 ctaaatgagg attgcagctc tttttgtttt acatagaaac gggaacagga aaaggcctgt
     9061 ttaggaaggc acaagcgcct tctcaattaa aaaacctttc tctcactcca accgccaccg
     9121 ccgttcgaga agaaaggaag tgaacgctgc acgttcacta agatatggtg tcttgcaaag
     9181 caagacaaaa taggaaaagc ttcctctcac caaaaggttc gttcctgttc gctatctctt
     9241 tattgatttg ccagaccggc aacaagttgc cttttatcac caaaaagcga caaggacaag
     9301 ggtaaatggc acgctgcgcg cccttgtcct tgtcgctttt cgttgattca ttctggccaa
     9361 caaatcaata aagaaagaac taacgaaagg aagtaaaacc tttcctaaga ggaggcagaa
     9421 tcaatcatgc gaaagaaatt tgattattgg ggtgttccct tcagcgaatt attcccaaac
     9481 tatcatgcac cacatactgt cgaatgcgat tgtggagaaa gagcgaaatg tatcaaaagc
     9541 tatcgtttgt atcaatgtcc aacatgcggg aaaaaatata ccttgtctta tggcgattac
     9601 attttgattg atgagaaagg aaagaaacgc tgatgaaaac acaacacaaa ttattgaatg
     9661 taaaagaagt catttctgaa aaagtagcag aagctttact tattccgcgg tactggacga
     9721 tagaaacccc tttagtagag gacgcaccga ctatgctatt tgaagaccta gtgaagattg
     9781 caggatatga actgcattat caagaaaact tggaaactta tgaaaacgaa tattggttaa
     9841 ccaaattaga gaactaaaaa ggagaaaaaa tatggctcag aattactatg acgaatttgt
     9901 caaattacct ttggataaaa tggcacaaaa aatggaggat atgacgttcc tataccatga
     9961 aacacgtgta cccaaaaaac attacaagga aaaattgtca gtcgcagtgg aagaaatgat
    10021 tgaatcaggt gtcgaaatga atctcattgc gacgtactat cgaacattgg aagaactgaa
    10081 aaaacaaaat ggaaaatggt ttttccaagc cttactatgc ttggaagcag gcgtcaaacc
    10141 aagcacaatc aaaccttctg aataccaagc tttagaacta acgtatggaa aatttatcga
    10201 aacgaaaaaa gccaagactg tttcaagtga atggttggac tactttgaaa atgtcaacaa
    10261 gtatggcgca tgttacatca tgaaaaagga ggacaacgaa aatgagtaat gtggtttatc
    10321 aactaacagt ggaagatgtt tctcactttt taacaaacaa agaacatttg aatgaagtag
    10381 tcagacggat tcaaaaagac tttagttgta tggaagaaat gaagtacttt attcaaagag
    10441 tggaagaaca gttgtatatg gaaagcgaaa cgagtcaaac gaaggtcaca gtcagccata
    10501 atcatttaga cgtgtataaa cgtcgtcctg aaacggtaaa aaaaggaaca gagcttctac
    10561 gactagaaac agaaaaatac atcatgaatg tgtacctaga aaggagtgag ctggtcgaaa
    10621 actttgtcag ctacacgatt tgtaggaaac actattactc agaatcagaa tattattcaa
    10681 tttttgagag catggaatta atggaagaag aaatggaaga gctaaacagc aaaaatgcga
    10741 cggtagacga ttattacctt gtatttacta gatttctgac gtgtgggacg gaatctatcg
    10801 tgttaatggg aatggattat gaactcgctg tacccgaagc atttttgagg gaatatgtag
    10861 aagaagtaga taacccaggc gacgactggt acacgacttt aactgatttt ttagatacct
    10921 acacttacga cgatacagaa gaaatccaaa atgagcttga ctgtaaacga atgccatatc
    10981 aacgatttga aaggaaaaaa ggtcaataac catttatacc gaaaagtgag aatgtgtgaa
    11041 ataagaacaa aaaaccttga aattaaggga atggttctta tttcgcacca atcgctccaa
    11101 ccgcccccgc cgctcaggtg gtgcgaaata agcgggcaac gaccgataac ctaagagaaa
    11161 gaaggaaatc catatgaata aaatcattcc tctgttgttc gtactgtttg ttggcgagat
    11221 acttatgcgt catgctgcag ctgtacattt aaacttcgta gtattcttag gaatactggt
    11281 attttgtttg atcttttggc gatttgtatt agcccttgtg agtctactat tcaagatgat
    11341 tgcagccata tttttcatag ggttatttcg atgaaaggag agagtactaa tgccaattgc
    11401 aataggaaat aaacgtttac ccgtgacgtt ggatgaaaag agacaaaaag aattgcagga
    11461 actcaaacag aagtacggca aaagtgaatc caggattatg tgtattgcgt tagatttatt
    11521 gattgcacaa gaaaaagcag ggtttgatgt accagcactc aaaaagtgac gtcacttttc
    11581 atttgaagaa acaaaagtga tagcacttta cgcacgtgtc gatacgtgtt atgattattt
    11641 tgtaaggagg tatagtgcac aatgccactt acgggaaaag aaatgcttaa gttattgaaa
    11701 aaaaatggct gggttgagcg aaggcaagag ggatctcatc atcatcttta taaggatggt
    11761 gttagaatca ctgttcctgt tcatgccaat caagatcttg gccgaggact tgagcaaaaa
    11821 attttaaagg atgcgggatt gaaataatct cgcattcgat taaagaaagg attgactgaa
    11881 atgttaagtg agcctttagc aaaaacctat cctgctattt ttagcccaga agaaggtggt
    11941 gggtacttta ttgaatttcc tgatgttcaa ggtgcttaca caggaattaa cgaagacgat
    12001 atatcttatg gaattgctat ggcacaagag gtgttaggta tggttttagc agattatatt
    12061 gagcatgaag atctacttcc agaaccaaca ccaatcaata agatttctgt tgaagatgat
    12121 tcttttacta ctttaattcg tgtagatgtt gccaaatatt tgaaagacac agaacttgtc
    12181 aaaaaaacct taacaattcc tcaatgggcg gataagctag gcaaaagggc tggcataaat
    12241 ttttcagtcc ttctaacaga atcaattgca gataaagcag ataacattct tcgttctgga
    12301 agaaataatt agaactcaaa agagtcagaa acttaaaaat ctttggcttc tttttttact
    12361 acaaagatgg cttctttaaa ttatttgcct gattgattta aaaaaattgc gcaaagacta
    12421 cgagctaatt aagttgctcg tggtctttct ttatgaaaac tttttagatc tagataaaaa
    12481 cattgagaca ctttgaaaaa tttttttaat agtaatgggc gcttctatgt caagctgatt
    12541 cttctttcgg taaattaatt ttacttagaa tatcactatc ttgataaaaa atggtgtatt
    12601 cacaaattct ggcataaaaa atacctactt tctagttaag gttcctgaat aagtcgcaat
    12661 ccaattattt ccagctgatt tataactttt taaatataat tttccataat accaataacc
    12721 attacttttt tctgtccaat ttcgttattg agaagatcta gaaggccagc caactttatc
    12781 aaaaatcatc attcctgtca ctcgtatcgt tttagaagct gctcgagtaa gtaatggaga
    12841 aactgttaaa taattttaag taacagttaa gtcgttagtt gggttatcaa tggctattcc
    12901 gtccttatct gaacctcgaa acccaatcat agaaaaagtt ttttgaacaa gttttaacag
    12961 ataaatcaag ttatatacta gaaaattcgt aaaatatatt tttaatgtca taatcgtttg
    13021 atttttctgg attgacattt atttataaaa aatagtgcca ttaaaaaaga aaagtgacac
    13081 accaataaaa gtcatatcct gtgacattat atggcattat ccaaatagaa taaccggttg
    13141 acatgtaagc ggattccttt tataatttta ggagtataag tccttttcta aaaatattaa
    13201 ccggttatca tgaaagggga tacaaatata tcgttggagg ataaaaatat gtcaaaggtt
    13261 aacgtattta aaaatcaatc ctatttacaa agttcagcta cattattact attttttgct
    13321 tcttggggtg tttggtggtc attttttcaa ctttggctaa catctgaatc aaatggttta
    13381 gggttatctg gcagtgctgt aggaacagta ttctcggcaa attcgttagt taccttaatt
    13441 ttgatgttta tttatggaac attacaagat aaattgtata ttaaacgaaa tttattaatt
    13501 tttgcttctg tattagcgac acttgttgga ccatttttta tatggatata cgggccattg
    13561 ctagataaca attttaattt aggcattatt atgggagcac tatttttgtc agctggatat
    13621 ttagcttctg taggagtttt tgaagctgtg tcagaaaggt ttagtcgttt atttggcttt
    13681 gaatatggac aagcaagggc gtggggatca tttggttatg ccttggtagc gcttttggca
    13741 ggatttttat ttgtaaaaaa tcctcattta aacttttggg cgggatcttt ctttggttct
    13801 ttactattgt taaatttatt attttggaac cctaaagttg aacgggaagc aaatcaaaat
    13861 tttaatcaag aacaagctga atcaaatagt attccttctt taaaagaaat gtttgatcta
    13921 atgaaactgc ctcaattatg gacgataatc atctttattg tttttacatg gacattttat
    13981 acggtattcg atcaacaaat gtttccggga ttttatactg gtttgttttc aacatcagct
    14041 aatggtgaaa aaatatatgg gacattgaat gctattcaag tattttgtga agcgttaatg
    14101 atgggaattg ttccaatcat tatgagaaaa ttaggggttc gaaatacttt gttattaggt
    14161 gtaaccatta tgtgtgtacg aattggattg tgcgggtttg cctcgacacc attatctgtt
    14221 tcatgcataa aaatgttgca tgctttagaa gtaccattat ttacattacc aatgtttcgc
    14281 tattttacac ttcattttga tacaaagcta tcagcaaccc tctatatgat aggatttcag
    14341 atagctgctc aaattgggca agtgatttta tcaacaccat tgggaatatt aagagacaac
    14401 gttggctatc aaccaacatt taaaattatt tctcttattg tattactagc aggcatatat
    14461 gcattcttta ttcttaaaca agatgataga gatgttcaag gggatccatt tattcgagga
    14521 taatggaaac caacagaata aaaaacaatt ttatctagaa agtgagcgaa tatattatga
    14581 caaaaagagc tatagaatta acaaatacac gttatcgttt gggatatcat gtgtctgcac
    14641 catcaggatg gattaatgat ccaaatggat tttgctattt tgatgggtat tatcatgtgt
    14701 tttatcaaca tcatccatat agtgctgaat ggggaccaat gcactgggca catgctagaa
    14761 gtaaggattt agttcattgg gaatctttac cacttgcttt gactcctgga gatcaagaag
    14821 atgaaggtgg ttgtttttca ggaagcgcta ttgaaaaaaa tggtgtgttg tatctgttct
    14881 atacaggaca tcactattat ggagacaatg atccagatca tttttggcaa aatcaaaaca
    14941 tggcgtatag tacggatggc attcacttta ctaaatatga aaaaaatcct gtaatagcaa
    15001 aagcaccaga agataacaca catcatttca gagatccaaa agtatggaaa cacgatgaaa
    15061 aatattacat ggttttgggt agtcaagaaa aagatggatt aggtcgtgct attgtttatc
    15121 agtcgttaga tttattaaat tgggattaca ttggaccaat ttctaaagcg aaggaagtac
    15181 ttacagaagg atttatgtgg gaatgcccag acttttttga attaaatgga aaatctattc
    15241 ttttattatc ccctcaggga attgaagcaa atggaaaaga ttatttaaat ctatatgaaa
    15301 caggctactt tatcggtgat tatgattatg agacagcaaa attcattaga ggaaattttt
    15361 atgaattaga taagggacac gatttctatg caacacaaac aactttatct ccagatggta
    15421 gacgtattgt tatggcttgg atggatatgt gggaaagccc tatgcctgaa aaagtagacg
    15481 gatgggcagg agcattaacg cttcctcgag aattagagtt aattggagat catttgtata
    15541 tgaaacctgt gagagaatta gaacaacttc gagttggcag cggcgttgaa acaaaagtta
    15601 atttggcagg agaaataata atcgctcaag atgcttcatt caaagaagct ctaattgatg
    15661 tactattgac taaatcaaaa aatcaagaaa tcaaattttc attaaaaaca aaagatgaag
    15721 agttaattaa tattttttat tctaaggata aaaatgaatg taccttaact cgtaacggca
    15781 aaaatgatcc aaggtatgga accattcaga tgtgtgatag attatcactt cgagtgttta
    15841 tcgataaaag ttcggcagag atttttatta atgatggcga gcttgttttt acagagcgtt
    15901 attatacaga aaatcaacca agtatgcatt tgagtgttag tgaagaagct tttgtctcga
    15961 gtacaattta tcatctaaaa aaagatgcaa tttcttacga gtaaggaggt tttatgatgt
    16021 taattggtag catagaagca ggtgggacta aatttgtatg tgccgtggga gacgaggatt
    16081 accgagtaca agaccaaata atttgtccaa cgaccacccc cagggaaaca ttgaaaaaaa
    16141 ctgttgacta ttttaaacag tttgatataa atgcattagg tattgcatca tttggtccaa
    16201 tagaaattcg aaaaaattca ccaaaatcag gatatattac gtctacacca aaggtgggat
    16261 ggcgaaatgt taatgtgtta ggttatttac aagatgagct gaatgttcca atgtcttgga
    16321 caacggatgt gaacggatca gcttatggag agtacatcat gtccacttta tcgaatgaaa
    16381 aaataaattc attagtttat tacaccgttg gtactggcgt tggtggtgga gctgttgttg
    16441 acggaaaact attaggatcg tttggtcatt cagaaatggg ccatacgttt gtaaaacgcc
    16501 atcctgatga tattgatttt aaaggaattt gtccttatca tggtgattgt ttagaaggct
    16561 tagttgcggg accaacgttt gaagcacgtt ttggaaaaaa gggtgaagac gtttctttaa
    16621 cggatcctat ttgggatgta atggcctatt atctagcaca agctgtgatt caaacaacat
    16681 tgattatacg accggataaa atagtatttg gtggaagcgt agtaaatgaa aaattgttag
    16741 aaaaaattcg tgttaatgtt aatgaattat tggatgatta cgtggaaata ccaccgttaa
    16801 gtaaatatat tacgcgtcct ttaattaaaa ataatggatc ggcaacacta ggaaactttg
    16861 ctttgggctt gaatcaatta caagaataaa acatagaaaa aatatcgatt atcaggtata
    16921 gtagttctat agagttcttt ttttcgatag ttaattattg cattatttag gagagacaag
    16981 agaatgcgcc caaaattaga agatgttgcc aaaagagcaa atgtatcaaa aacaactgtt
    17041 tcacgtgtat taaacaatcg tggctattta agtcaaaaaa cgattgataa tgtttataag
    17101 gctatagaag aactcaatta tcaaccgaac gttgtagcgc gtcaattatt tcaaaagaaa
    17161 actaacattg tcgggttact atttcctaca gtagccaatc ctttttttag tgaactcgtt
    17221 gaggcattgg aaaaaaagtt atatgaaatt ggttataagg tattaatagg caattcgatg
    17281 aataataaag aaaaggaaac aaattattta aatcagttat tgtctgatca agtagatggt
    17341 ttaattgttg gtacacataa ccaagggatt caggaatata aatatcagaa tttaccgatt
    17401 gttgcaattg atcgagtgat gaatgaagat attccagttg ttgaatccga taactacaat
    17461 ggtggaaaac ttgcaaccaa gttattaatt gcacaaggtg caaaaaatat tattcataca
    17521 aacggtccaa ttgacttaca gactccggct aatcgtcgga gactagccta tgaagatact
    17581 atgaaagcat atcagttaat tccacgaact gtgacattag atttcaatat ttcttatgta
    17641 aagaaaaaac agattttttt ccaaatgttt gaggattatc ctaaaataga tggtattttt
    17701 gcttcaaatg acatcgatgc ggccctaata ttacaagtag caaaagaaaa ggggttaaat
    17761 gtaccagctg atttattagt agtcggatat gatggtactt taatgactcg gagtatatta
    17821 ccagatctga caacagtaat acaaccaatt aatgatatag cagacacagc tgttgctatt
    17881 ttgatgaaac gaataaataa agaagaaaca aaaaaggaat atattttacc tgtcacatta
    17941 tggatgggga agacatcagt aaagaaaaag gacaattaaa tttataaaga ctatcaatga
    18001 taatttcatt attaattcgt gatataaaaa gttgttaaat ctcttataat agactatgat
    18061 acaaaatcat gcttttaatc tgtgtagatg ttgccaaata tttgaaagac atagaacttg
    18121 tcagaaaaac cttaacaatt cctcaatggg cggataagct aggcaaaagg gctggcataa
    18181 acttttcagt ccttctaaca gaatcaattg cagataaagc agataacatt cttctttctg
    18241 gaagaaataa ttagaactca aaagggtcag aaacttaaaa gtttttgatc ctttattttc
    18301 aagtctgatt tgacagattt tcggcagctc atttatttat ggtcagaaat agttgctgaa
    18361 attttaatct attagcaaat agtccagaac gtatgaagag gattcggata tcatttgtca
    18421 ttaacaccat aaatatttat aaaattcgtg ttcacctaca attaaattta atatataata
    18481 ttcgttgtaa gggcttataa ttttttgtta atgaacaaga tatgtaattg atagtgtcaa
    18541 tagctattca aaaatgtagg cttttggccc ataaataaca aggttactta tatgataact
    18601 ttttttcttt ataatcttta atccgataga gctttccagt agtttgaata atcttagcgt
    18661 ggtgcactaa tcgattgatt agtgcatttg tgttcagtcc attgagacat aatagtggat
    18721 agttttactg agaaaattga taaaaaatag agatataatt caaatacgta tataatgaaa
    18781 ctaaagataa atacggagga aaatatatga ttataaaaaa cttaggaaat gttaaatcaa
    18841 tagatataca cgttaaagat ttgctgattt ttactgggaa aaataatacg ggaaaaacat
    18901 ataccagcta tattttatat ggaattttat cagaactctt tggagcaaga tttttcaatt
    18961 atttttctca tacagaaatt gaaccacatt tacaaaatag tttaaatatt ttattagtta
    19021 gcaaagactc tataaggaaa aaatttaata atgatgcaaa aaaattttta gaagaaaatt
    19081 taaaaaaaat tgcagttcgt aactttaaag taagtgaaaa taacttcaaa aatctttctt
    19141 ttactatttc agattcagaa attagtgagc tcactggtga tatctttaat tatgagatca
    19201 aaagatacag tataagtggc attgttataa caattcaaaa tgtaggagag aaattaaaaa
    19261 ttagctttga gccaactgat tcatttcagt atcttagcaa agatattgaa attgaagata
    19321 tttataaaat tttagctcaa gagctatcta aaaatgttat tagtttgcca aaggtttttt
    19381 attttccagc cgaaagaact ggtataaatg tctttaagaa cgaactaaat gaaagtcgct
    19441 taaaaacata tgataccttg ctgaatactg tgcaatttac taatttaact aaccaaaaaa
    19501 agaaagctgc cctcaagtac gatctgttaa aaaagaatat tgagctgtta atggagacaa
    19561 cggataatag ttcttatcca aagccaatat cggattatat taattatctg aataacatga
    19621 aaaatagata tgaacagaaa aatgtcaata atatagcatc atatattaga gatgagatta
    19681 ttatgggtaa atatgaaatt gacgaaaaag ataattctgt aagttttaga caaaagtttg
    19741 gtaaaaatag atatagaaaa gaacgaattc cttttcacgt tgtgtcttct tcgattaaat
    19801 ctctatatgg attagattat tacttagaaa attcaagtaa agaaggagac tatcttatta
    19861 ttgatgagcc agaattgagt ttacatccag aaaatcaggt taagttagca aaagtaatta
    19921 cagatatagt tgagagtaaa attaagctaa ttacttctac ccatagtgat ttatttgtac
    19981 gtgagcttac aaatattgtt attgaaaata aattaaatag taagaataat aaaaaaaata
    20041 aattgtctga taaaaatata ggcatatata attttgtagg aaataatgat ataaatgaat
    20101 tagaaaaaat tt
//
LOCUS       DACECY010000051        19512 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.055, whole genome shotgun sequence.
ACCESSION   DACECY010000051 DACECY010000000
VERSION     DACECY010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.055"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            127..3654
                     /locus_tag="IWO38_001980"
     CDS             127..3654
                     /locus_tag="IWO38_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0314063.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="IWO38_001981"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="IWO38_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ0314064.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="IWO38_001982"
     CDS             7442..7783
                     /locus_tag="IWO38_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0314065.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="IWO38_001983"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="IWO38_001983"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ0314066.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="IWO38_001984"
     CDS             9140..11560
                     /locus_tag="IWO38_001984"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ0314067.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="IWO38_001985"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="IWO38_001985"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ0314068.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="IWO38_001986"
     CDS             12994..13731
                     /locus_tag="IWO38_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0314069.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="IWO38_001987"
     CDS             13745..14560
                     /locus_tag="IWO38_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ0314070.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="IWO38_001988"
     CDS             14573..15220
                     /locus_tag="IWO38_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0314071.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="IWO38_001989"
     CDS             15236..15892
                     /locus_tag="IWO38_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0314072.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="IWO38_001990"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="IWO38_001990"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ0314073.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="IWO38_001991"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="IWO38_001991"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ0314074.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="IWO38_001992"
     CDS             18234..18752
                     /locus_tag="IWO38_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ0314075.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /locus_tag="IWO38_001993"
     CDS             18859..19359
                     /locus_tag="IWO38_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0314076.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6564 a         3348 c         4186 g         5414 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggacaaaag cgtttaactc caagaaataa gaaggaattc acgaaaattg cttttcaaat
    19501 ttttgtgaat tt
//
LOCUS       DACECY010000052        18840 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.056, whole genome shotgun sequence.
ACCESSION   DACECY010000052 DACECY010000000
VERSION     DACECY010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.056"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(21..251)
                     /locus_tag="IWO38_001994"
     CDS             complement(21..251)
                     /locus_tag="IWO38_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314077.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="IWO38_001995"
     CDS             complement(687..1253)
                     /locus_tag="IWO38_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0314078.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="IWO38_001996"
     CDS             complement(1253..2209)
                     /locus_tag="IWO38_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ0314079.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="IWO38_001997"
     CDS             complement(2209..2865)
                     /locus_tag="IWO38_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ0314080.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="IWO38_001998"
     CDS             3235..5253
                     /locus_tag="IWO38_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0314081.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5680..8094
                     /locus_tag="IWO38_001999"
     CDS             5680..8094
                     /locus_tag="IWO38_001999"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ0314082.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="IWO38_002000"
     CDS             8442..9995
                     /locus_tag="IWO38_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ0314083.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="IWO38_002001"
     CDS             complement(10376..12160)
                     /locus_tag="IWO38_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0314084.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="IWO38_002002"
     CDS             complement(12175..13896)
                     /locus_tag="IWO38_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0314085.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..14757
                     /locus_tag="IWO38_002003"
     CDS             13996..14757
                     /locus_tag="IWO38_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0314086.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15724..15992
                     /locus_tag="IWO38_002004"
                     /pseudo
     CDS             15724..15992
                     /locus_tag="IWO38_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16664..>18840
                     /locus_tag="IWO38_002005"
     CDS             16664..>18840
                     /locus_tag="IWO38_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314087.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
BASE COUNT         6186 a         3343 c         3771 g         5540 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14581 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14641 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt gacgaatacg
    14701 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14761 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14821 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14881 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata tatacgatgt
    14941 aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt ataattcatg
    15001 gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt gtatcagatg
    15061 agaatgataa attaaatagg tgcataaaag cattacactt aaaatacatt gttgcttaca
    15121 ttattacaat atgttaataa ggcaaaaata aatagatgga ttgaataaac atatttttta
    15181 atcaaatagt ccctactgtg actttcctca gctatcggtg atactaccga tagcattttt
    15241 ttgcttataa aataattata agtatttaga gatttttctt ctaacattct tcaaacaaga
    15301 aaaatcacaa aggttattgt tatttttata tattcttttt aagaatgatt gtttttttat
    15361 agaaatttta aaaaataaaa cacgttattg atggatagtt agtttgttat ttggtaatat
    15421 tatagaagtt gactggtagt gatttatttt tcgtcgtttt ataaaaatgt tattttttgg
    15481 aaaaattcac aaatatgtaa cgaatgtttt tctggaattt gctgtgttaa aaagcagtat
    15541 gactaatata aatactattg tcatttatgc aattttttat ggatagtaat tttagagggg
    15601 aatcaatgaa ggaagaatag aagggaaaaa tagttgcaaa tacgccagtt agcaaaaata
    15661 gcaatacatc ttcaagcttt aaaattcgag agaatcatct tggtagtgga atcaactaca
    15721 acaaaaggat taaaccaatt aatagataga attaccaatg gaaaaataga aaaggtagtt
    15781 attctataca aaagttgttt aattcgcttc ggttatgaac ttagtgaaac cctttgcaat
    15841 atatggtaca gaatatagaa attattgaca ttacagaaaa tgatgaagat ttaatacaga
    15901 ttgtcactat ttgtagatac cttttacaag gaaaccgagc caataaagct aagaagatga
    15961 ttaaggagtt gatggaagac gatacaagtt aagaaagtga aactacgccc aaataaagag
    16021 caaaaagagt aattggacgt ggaaaatgca agaaatgaac catcgattcg gtggacagtt
    16081 tattagtaat gacggtttga gaacatccat gacgagaatg aaggaacatc ctaaatgcgt
    16141 ttagttgaac caagtgccaa ataatatggc caaataagcg atcagttatc gatgaatccc
    16201 aagtattcta aactatatat tcattttgat gctaagcatt gatatatttt tttgggattg
    16261 atgaaggtta accaatacag ggactcactg atgaaattct agagtctgac cttggtatca
    16321 acgatttagt gattgtttct aatggaaaag tataaaagta tataagtaca ttaacaaaat
    16381 aactattagg gttacgctcc agcttgtgca aggcactggc aggaggaatc gtgaaatcat
    16441 aattatggtt aagccacacg gacaactaaa agtcatacaa agctatcgaa tcaatcaaca
    16501 gaagacaatc ggcaatcatt agctttgttt acagcttaca ttgcaacgct tgagttgaac
    16561 cgaaaatacg agggaggtaa cgcagccatt ttgagttggg gaatcttttt agaaaattat
    16621 cgggtacctt gttgtttagt caaaattata agggggagta atagtgagga acaaatttca
    16681 taaactgtta agtatgtttc tagttatgct tattgtgatc aatcaatttg ggaacataac
    16741 ggttctagct gagtctttag cagaacagcc agagcgttcg ctgacgatcg aagttgatca
    16801 agaggaggct gctttgggtg agcagttcca gttgcgagtc agaggaacac cagacctgct
    16861 aaatgcattg gtcattgata agagtgagga tttgattgaa ggggaagtga agcaactcaa
    16921 tgattacgaa cgtctgatta cttattcttc ccagcattca ggtaaatacg aattgaccgc
    16981 aagaagcggt gatattcaat caaatacggt ctcaatagaa atcagtgtac cagaagaaag
    17041 cacaagtgag tcttttaatg taccagaaga aagcacaagt gaatctttta atataccaga
    17101 agaaagctta agtgaatctt ttaatgaagc cagatcatct gagccatcta taacgcagga
    17161 atctcagatt gaaagacaag aggagctctt gaattatgac atcggcatct ctgctgagcg
    17221 ccccactttg ccgagcaggc atacacaaac gttaaaggga tacattcaag gaggcgctgg
    17281 cgatattctt gaagaatatg gggagatggt cgtacgagtt cctagagcat cgattggctt
    17341 aattgagttc attagagtgg aggtcgctga tgattcacct ttaaagccac tggcatttta
    17401 ccaagaaggc acagacgttg tttttccatt tacctttgat tatgaaggat taaatgaagc
    17461 cgctcagtcg tatacattca gtatcacgtt tgaagttgct ttctttcaat caggcggtac
    17521 tgagccaaat aatgactttc aagtgagtct tacttatgct gagcgagcag caaacacgga
    17581 attttcaatt acgcctattc cttcatcaag acccattttt tcgaaattat actatggctc
    17641 tagagaaatc gacagccaag gaaatcttgg gaatctgctg attgacgggc aagacgtgac
    17701 caacagtaat aaatttattt tgcaggtaaa ttatgaagga tcacgaaaag acggtgtagt
    17761 ggttaccgat gtactaccgc aaggaacaac tttggcaccg aatccgacta catggagttc
    17821 cactggaaat agagaaccta tttcaggtgg ggttcggatc gcagaagtaa cgaatccagg
    17881 tgaagcaaac gttagaactc attatgtttc cgaggaattt gtggacaaag tatcttttaa
    17941 taaggaaacg aatacacttg ttgtggactt tggtaatcta gcagagggtc aatcgtattt
    18001 tattgaatat gctcttgaag tgaccgatgt cgacttgttc aatgaaggaa gacaagagga
    18061 tgggttttct cggaatcgag cgagtatgac ctacaataat caggtaatga gccgagacta
    18121 ccgcttgaga atccctgaaa gtaatcttcc ggcaatctct ttaagaaaaa gtgtggacaa
    18181 aaatgttatc aatctcaatg aaaatgaact aacgtatact ctgcatgtct cgatgttttc
    18241 tggaacaaga aatacgcttg ttattgaaga tgttttggaa gaaggcatgg aatttgtgtc
    18301 atttctatca gatattccct ctttcattga tgccgaagtc aataatggaa tagttcgttt
    18361 cagtgcgaat aatacgattg gtggtccgac aagttttacc ctctctttta aggtcaatgt
    18421 agaggcgtat gcggtcggcg atcaaatttt aaactttgca acgcttgtga atagtgacaa
    18481 tcgattgaat acgagtactg taagaacaag gaaaatcgat gggcgtatta aagtaattaa
    18541 aactgatgag aatggccaaa ggctggcagg agcaaaattt gaaattttat caagcgattc
    18601 acaagtgata caagaaggta taacggcaga aaacggagaa tttttaagtg aaccgcttcc
    18661 tgtaggtagc tatcaagtcc gagaaacaga agcaccggaa gggtatcttt tagatgcgac
    18721 ggtacatgat gtttttataa atgaatactc catagaagaa gtgacgttaa ccattgaaaa
    18781 catccttgat acgggtggtg ttgaactaat taaacatgct gaaggcagtg gtgaagtcct
//
LOCUS       DACECY010000053        18407 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.057, whole genome shotgun sequence.
ACCESSION   DACECY010000053 DACECY010000000
VERSION     DACECY010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.057"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(37..846)
                     /locus_tag="IWO38_002006"
     CDS             complement(37..846)
                     /locus_tag="IWO38_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0314088.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            1139..2575
                     /locus_tag="IWO38_002007"
     CDS             1139..2575
                     /locus_tag="IWO38_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ0314089.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNEVSVALWIVDILFIINFIILATI"
     gene            2745..3941
                     /locus_tag="IWO38_002008"
     CDS             2745..3941
                     /locus_tag="IWO38_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0314090.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSESTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFILSNIFLSLFNFRK
                     VLRSRL"
     gene            complement(4003..4188)
                     /locus_tag="IWO38_002009"
     CDS             complement(4003..4188)
                     /locus_tag="IWO38_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ0314091.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            4415..7729
                     /gene="dnaE"
                     /locus_tag="IWO38_002010"
     CDS             4415..7729
                     /gene="dnaE"
                     /locus_tag="IWO38_002010"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ0314092.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLIKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLIENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKDGSQIPLENLSASLPLT
                     ANGYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITNYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLGPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            7897..8859
                     /gene="pfkA"
                     /locus_tag="IWO38_002011"
     CDS             7897..8859
                     /gene="pfkA"
                     /locus_tag="IWO38_002011"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ0314093.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            8933..10717
                     /gene="pyk"
                     /locus_tag="IWO38_002012"
     CDS             8933..10717
                     /gene="pyk"
                     /locus_tag="IWO38_002012"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ0314094.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(10752..11654)
                     /locus_tag="IWO38_002013"
     CDS             complement(10752..11654)
                     /locus_tag="IWO38_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0314095.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            11840..12409
                     /locus_tag="IWO38_002014"
     CDS             11840..12409
                     /locus_tag="IWO38_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ0314096.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            12467..12646
                     /gene="rpmF"
                     /locus_tag="IWO38_002015"
     CDS             12467..12646
                     /gene="rpmF"
                     /locus_tag="IWO38_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ0314097.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            12816..14237
                     /gene="gndA"
                     /locus_tag="IWO38_002016"
     CDS             12816..14237
                     /gene="gndA"
                     /locus_tag="IWO38_002016"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ0314098.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDEAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            14430..15116
                     /locus_tag="IWO38_002017"
     CDS             14430..15116
                     /locus_tag="IWO38_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0314099.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            15113..16618
                     /locus_tag="IWO38_002018"
     CDS             15113..16618
                     /locus_tag="IWO38_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0314100.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(17242..17385)
                     /locus_tag="IWO38_002019"
     CDS             complement(17242..17385)
                     /locus_tag="IWO38_002019"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314101.1"
                     /translation="MEKEAFDLHVLGTPPVFVLSQDQTLIKSSNNLAIVKLNLFASIL
                     LAC"
     gene            17305..>18407
                     /locus_tag="IWO38_002020"
     rRNA            17305..>18407
                     /locus_tag="IWO38_002020"
                     /product="16S ribosomal RNA"
BASE COUNT         5893 a         3247 c         3959 g         5308 t
ORIGIN      
        1 tgtcacaacc tcttttttgt ggaatctaat tctctattat agtttaagta gtttttccag
       61 ataaacagct aaaccgtctt cctgattgct caaaggagtg acatcatttg caatgccttt
      121 gagttggtca gtaccgttag ccatagctac tccccaacct gcataatcca acaattccaa
      181 atcattatgc tcgtcaccaa aggcaatgac gtctttttga tcgatagaca ggtagttagc
      241 aatctcttgc acgccttttg ctttttggat tccttttgaa acaatctcta agatggcagt
      301 aggtccaccc catgtccgaa catcgacata actgccaaat tgacgtgtta attcagcaga
      361 tacagctcct gcaaaacgtt tgtctgttcg gacaagcaaa gatgtaggat ttgtcgtcaa
      421 atttttagga gaaagcaaat tcttctctcc tggacgagaa gaagcaaaaa ttttttcatc
      481 aaagaaatca aaactgtcaa taaaaaaagt atcacgattt tcagcagcga taaaatctaa
      541 atttagctgc tttttttgcg ataagatttc aaaagcgatt tcacgattga tcaacgtttc
      601 tctttcacca tcccagtgct gattagggat gtgtactaat gcgccgttaa aattaaccat
      661 cggtgttgtt aattctaatt gttggtaaaa ctgatggctc attcggaatg ggcgtcctgt
      721 agcaatactg acatgatgac cggcattgat tgcttttttt agagtctctt ttgttttaga
      781 tgtaatcatc gaatcttgat tgagggtagt tccgtccaga tcgatggcaa ttaatttttt
      841 attcaataag ggtccttcct tcctcaacta gcatttgttt tagtctagca tattttaggt
      901 aattaacaag agcaatcagc tattcctaac aaaaactaca gttatggcta tataaatagc
      961 taaacataag ctaaacacga acgttaagta ttattttcgc tttttttgtt aaaaaaacgt
     1021 tgaaaagcga atgaaacgtc tatataattt gaaattgttc gatggatttg ccctatttta
     1081 cttatttttt attattatag ttttccatca gaaaaaaatt cagggagtgg aactttttat
     1141 ggaaaagttt ttcggtttaa agaaaaacaa tacaaatgta tcaactgaaa taatggcggg
     1201 agtaacgaca ttctttgcca tgagttatat cttattcgta aatccgacga ttttgtcagc
     1261 atcaggtatg cccttccaag cagtatttct agcgacaatc attgcatcga ttattggtac
     1321 gctggtaatg ggattgttcg caaatgtacc atatgctcag gcacctggta tgggattgaa
     1381 cgcatttttc acttttacgg ttgtttttgg gcttggttat tcttggcaac aagccttggc
     1441 aatggtgttt atttgcggat taatcaatat ttttattact gtcacaaata ttcgaaaaat
     1501 gattattcgg gcgattcccg aaagtcttca acatgcgatt ggtggcggga tcggtatttt
     1561 cgttgcgtat gtcggaatca aaaatgcagg attcttatct ttttcagcag accagtcagc
     1621 tatttcgagc tctgtcgttg aaggcggaaa agcgacaaat gttacgataa atggtggaat
     1681 cgtccctgcg ttagccaact ttgacaacgc tcctatctta ttggctgtga ttggtttggt
     1741 actgacagcc attttagttg taaaaaatgt ccgtggggct attcttatcg gtatcgtagc
     1801 gaccacagtc cttggtattt ttatgggtgt tgttgattta agttccattg attggcaaac
     1861 gaattcgtta ggtaattcat ttaaagaatt aggaacaact ttcggtgcag catttggttc
     1921 tgaaggcatg caatctttat tcagtgattc ttccaagatt cctcaagtat tgatgacgat
     1981 catcgcattc agtttatctg atactttcga tacgatcgga acatttatcg gtacaggacg
     2041 ccgcacaggc attttctcaa aagaagacga gatcgcgttg gaagatggtc gtgggttcaa
     2101 aacaaaaatg gataaagcac tatttgccga tgcgatcgct acttccgtgg gggctatctt
     2161 tggtacatcg aatacaacaa cttatgtcga atctgcagct gggatcgggg ccggtggacg
     2221 tactggtttg acttcagtag tagtggcagt gctattcgca ttaagtagtt tattctctcc
     2281 actgatcgcc attgtaccaa atcaagcaac tgcgccagca ttgattttag ttggagtaat
     2341 gatgatggct tcttttgcag acatcaaatg gctagatatg gaagaagctt taccggcctt
     2401 ctttgcatcg atctttatgg gattgtgcta tagcatttcc tatgggattg cagcaggatt
     2461 tattttctat acaattgtga aagtaatcaa aggaaaatac aatgaagttt ctgttgcttt
     2521 gtggatcgtt gatattttat tcattatcaa cttcatcatt ctagcaacaa tttaactaaa
     2581 gagaagggat tggaacagta tttgttctga tctctttttg ttttacataa aaagaacgtt
     2641 acatataaaa tgacctttgc gggatttttc tctttttact ggcgagatcg ttgcttgttt
     2701 attcgaaaat aggtatgata aaagagacga aagagggatg atagatggag attaattgga
     2761 gaaaaaattt agtgattgcg tggattggct gttttttcac tggtgcaagt attagtttag
     2821 ttatgccatt cataccagtt tatgttgagc aactaggaac accaaaagat caaatcgaat
     2881 tgttttcggg actagcaatc tctgtgactg cttttgcatc tgccgtggtt gcgccaatct
     2941 gggggaatct agctgatcga aaaggacgaa aactcatgat gatccgtgca gcagcaggga
     3001 tgacattaac aatgggatct ttggcttttg taccgaacgc gtattggctt ttgatcatgc
     3061 ggttttggaa tggaattttg tctggatata ttccaaatgc cacagcgatg attgcttcac
     3121 aggcacctag agaaaagagc ggatgggcgt taggaacatt gtctaccgga gcgattgctg
     3181 gaaatctgat cggaccttca atgggcggtg cactggctca gtggtttggt atggaaaatg
     3241 tattcttgat cacaggggct ttgttgatga tcacaacggt attgaccatt ttcttagtca
     3301 aagaagattt ccatccgatc gagaaaaaag atctgataag caccaaagaa attttttcaa
     3361 aaatggatca tctctctatt ttgatcggtc tcttcattac gaccttgatt ttacagatag
     3421 gaatcactac gatcagccct attttgacct tatatattcg tgaattgagc gaaagtacag
     3481 aaaatatcct ttttgtcagc ggcttgattg tttcgatagc tggtgtttcc gctgtttttt
     3541 cttcaccgaa actaggaaaa ctaggggata agattgggaa tcaaaaagtt ctaattggag
     3601 gactcatttt atcattttgc tgttatttac ctatggcatt cgtcactaca ccgctccaat
     3661 taggtatact tcgctttatt ttaggctttt caactggcgc tttgatgcct tctgtcaata
     3721 cactgatcag taagattacg cctcaagaag gagtcagccg aatctatagc tacaatcaga
     3781 tgtttagcaa ctttggacaa gttttaggtc ccatgctagg atcgacagtc gcgcatgctt
     3841 ataattacag tgctgttttt attgtgacaa gtttgtttat tctgtcaaat atttttcttt
     3901 ctttattcaa ctttcgtaaa gttcttcgca gcagattata atggtggtga ggagcagaaa
     3961 ataaaaaaga gcttgagata tctcaagctc ttggctgttt ttttacgaat gggcagaggt
     4021 ttcttttctt ggcgcaactg caccaatcaa aaggataatg acacccgcaa tgaatgaaat
     4081 gattgttgtc aaagtaaaat catatgttcc gcttgttaat gcagcaccaa tataaccgac
     4141 tacttgacct agaacgacag cccagaataa tgtaacgata tatcgcataa agaacacctc
     4201 ttttctatct taattttacc agattttctt tgaatgtaaa gaaacgagac caaatctcaa
     4261 taaaatttga gttcttgcga aagaactcat tcctacagat tacaagcaaa tgaagtggaa
     4321 taatcaactt cagcgcctgc tatcttaatg aaagtaaaag gatcaaacct tttaacgcta
     4381 gtgaatttta acgaaaaaaa aggcggaatg caagatgttt ttcccacaac tttatacaat
     4441 tacttcttat tctcttttac aaagtacgat acgtattcga gaatatgtac aagaagcaaa
     4501 aaaacaaggc tacacttcat tagccataac ggataaagat gtcttatacg gtgcacctga
     4561 attctatcgt atttgtgtag aagaaggagt acagccaatc attggattac atcttagtta
     4621 tacttatgaa gaaaaaaatt atggcttgct tgcgtacgca aaagatgaaa taggttatca
     4681 gcagctgata aaactttcca gccgaaaaat gatccaagaa aaattggaac ttgcagaaat
     4741 gacagaaacc gaccatctag cgtttgttgt tccggcaaat cattctgttt ctgaggcttt
     4801 caataaagag gataactttt tcggtcattg gcagttttta cttgaaaatg tttttactag
     4861 aaatttttat atcggcttga tagaaaacac gcctgatcaa ttgcctgaat ggtatcagta
     4921 tgtgataaaa gaaaattatt cgctttgtgc attacatccc atcgctagtt tacaacaaga
     4981 agaacttttt gcacttgaag tgatgaaaca tatcaaagat ggaagccaaa tacctttaga
     5041 aaatttatct gcatctttac cactaacagc aaatggctat cttctttcag aacaagaaat
     5101 gatcaaacga ttcgaaaaag cgggtctttc ccaagctgta ataggcgccc agcgtttagc
     5161 tgaatcgatt tcatttgaat tccagttcca tcagaaatta cttccgcatt atccagttcc
     5221 taatggaaaa caagctggag aatatttaag agagttatgc tattcgcaag tggaacgccg
     5281 aatcactaat tatacaaagg aataccaaga acgtttggac tacgagttat ctgtcatcca
     5341 tcaaatggga ttcgatgatt atttcttgat cgtatgggat gtaatggctt ttgctcatag
     5401 aaacaaaatc gttaccggtg caggtcgtgg ttctgctgcg ggttcattag tagcctatgt
     5461 gttagaaatc acagatgtgg atccattaga atatgatttg ctgttcgaac gctttttaaa
     5521 ccccgaacgc tttacgatgc cagatatcga tttggatatt cctgacaatc gacgtgaaga
     5581 agtattgaat tatgttcgag aaaagtatgg acaatatcat atggcacaaa tcgcaacatt
     5641 tggcacaatg gcagcaaaaa tggttcttag agatgcagct agagtgtttg gtttatcgca
     5701 aagtgaagca aaccgctggt caaaagcaat acccaatcaa ttgaaaatca cattgaccca
     5761 agcttataaa acttcgcatc aattagtaga attagttcag gcaagcgaaa aaaaccaact
     5821 tttattcaaa actgcagtga ttttagaagg attgccaaga catgtttcga ctcacgcagc
     5881 aggtgtagtt atcagcgata agaatctact tgaactagtt ccgctccaac gcggttctga
     5941 tgaagctttt ctgacccagt atacgatgaa tgaggtagaa gcaatcggtc ttttgaaaat
     6001 ggacttttta ggactacgca acttatcgat tattgatttt actttgaaag ctgttaagcg
     6061 agtagaaggg tatgagatcc gattaaaaga gatcccgcta aatgatgcaa aaacgctgct
     6121 cttgtttcaa cgaggcgaaa cctcaggagt atttcaattt gaatctgctg gtatccggaa
     6181 cgttttacga agattaggtc cagaaaacat cgaagatatt gccgcagtca atgcattgta
     6241 tcgaccaggc cctatgcaaa atatagatac cttcattgcc aggaagaaag gaaaagaagc
     6301 cattcgctac ccagatgatt cactgatccc gattttgaaa aacacatatg gagtgatcgt
     6361 ctatcaggaa caaatcatgc agatcgcctc gactatggct ggatttacat tagggcagtc
     6421 agatattctg cgacgggcag tgagtaaaaa gaaaaaagac gtattagatg aagaaaagtc
     6481 gcattttgta tcaggagcat tacaacaggg atattcggaa aaaacagcag cgaccgtata
     6541 tgaatatatc gaaaaatttg caaactatgg attcaatcgg tcacatgcat ttgcttattc
     6601 attcgtggga ttccaaatgg cgtatctaaa agcccatcat ccaggtgctt tctttaccag
     6661 cctgatgaat tctgttcggc ataacacatc aaaattgaaa gaatatattg cagaagcacg
     6721 taaaaataaa ctcaaacttt cctcgccgtc cattaatcaa agtaattcca gttttgagtt
     6781 ggtcaataag gaagaaatca gattcggact gacagcgata aaaggtattc ggcgtgattt
     6841 tgtagaagat atattaaacg aaagaaaaca aaatggcccg ttccagtcag tggatcaatt
     6901 cctcatacgg atcgataagc gttggctgaa attagagtta ctgcaatcac tgacagcagt
     6961 aggcgttttt gatgaattat ctcctaacag gaaacaattg atgcttgatc tcgaaggaaa
     7021 aatccaaaat atcgtttaca gtggtggaag tcttgacttg ctgggaatca tggcattaaa
     7081 agaagaagaa gtcgctgact atacactaga agaacgcttg aagctggaag aggagtactt
     7141 aggtatttat ttgtcaggtc atcctgcaga agggtttgat cgtcttcgtt tagctaaaca
     7201 agttcagctg attacagata tcgtccctaa tcaaccagtc aatttattag tgatgatcaa
     7261 ggaagttaga gaaattcgga caaaaaaagg agaactgatg gcctttgttg aaggaaccga
     7321 cagtagtgga gaaatttctg tcacagtttt tccaactctt tatcgtaaat atcgtaattt
     7381 gtttgccgtt gatcgagtga tctttgttca agggcggagc gaaatcagca aatataatca
     7441 ggaactgcaa ctaattggag aaagctttgc tgatcctaac cagttagaag cccagtatcc
     7501 ttcacaaact tgctatttgc gtattacaga agaacgggaa gatcccgaga taatgaaaca
     7561 attacaagtg atttttcaga aacacccagg atatatacca gtagttatgt atcacgaaaa
     7621 aacgcaacga aaagtagttc tgtcagatgc ttattgggtg gatgacagca agagtataaa
     7681 ggggcaactt atttatctgc ttggcgaaga taatgtggtt ttcaaataac ttacggtaaa
     7741 aaatactact tgctttcatt ttttttcatt tttttatccg taaatcaaga caagtgagaa
     7801 aaataatgtt agaatgttag tcgtggttaa cgcaaaaatg gttgtaaacc taagtcatac
     7861 ttaggataaa gtattttaat gaggtgaatg tttattatga aacgcatcgg aattttgacc
     7921 agtggcggag acgcacctgg aatgaatgca gctgttcgtg ccgttgttcg taaaggtatt
     7981 tacgaaggac tggaagtata cgggatcaat tatggttttg ccggtttagt tgctggagat
     8041 attcgtcgtc ttgatgtggc agatgtaggt gacaagatcc aacgtggtgg tacattcttg
     8101 tattctgcac gttatccaga atttgcaact gaagaaggtc aattaaaagg aatcgaacaa
     8161 ttgaaaaaat tcggaatcga aggtctagtg gttatcggag gagacggctc ttatcatggc
     8221 gcaatggctt tgacaaaacg cggtttccca gcagtaggta ttcctggaac aatcgataat
     8281 gatattcctg ggactgattt cacaatcggt tttgatacag caatcaatac agtactagaa
     8341 tcaattgacc gtattcgtga cacagcgact tctcatgttc gtacattcgt tatcgaagta
     8401 atgggacgca atgcaggaga catcgcttta tggtcaggtg tggctggcgg agcggatgaa
     8461 atcatcatcc cagaacatga ttttgatatg gcttcagttg ctaaaaaaat ccaagaaggc
     8521 cgcgatcgcg ggaagaaaca ttgcttgatc atcttggctg aaggtgtaat gggcggaaat
     8581 gaattcgctg agaaactttc agaatttggc gactatcata cacgtgtatc tatcttggga
     8641 cacgttgtcc gtggtggagc accaagcgca cgcgaccgcg tgttggcaag taaattcggt
     8701 gcttacgctg tcgaattgct tcaagaagga aaaggtggat tatgtgtcgg tatccatgac
     8761 aacgaaatcg ttgcgtctga catcattgat acacttgaaa acaacaaaca taaaccagac
     8821 ttatcactat atgatctaaa taattcactt tcattttaag agtgaaagaa accaagctat
     8881 ttaagctgtg tacagcttta aataaacaaa attgaatggg agagtttttt aaatgaaaaa
     8941 aaccaaaatc gttagtacat taggaccagc aagtaactca gtagaaatta tttctcaatt
     9001 gattgaatct ggggcaaatg tcttccgctt taacttttct catggtgacc atgaagaaca
     9061 attatcacgt atgactatgg tccgtgaagc agttaaaaag acaggaaaag atgtcggtat
     9121 tcttttagat acaaaaggag ctgaaatccg tacaactgtt caaggcacaa cagaagctga
     9181 ttttggtcgt gcaggataca tcaaatttga agtaggagat caaacacgca tctctatgga
     9241 tcctgaacat gtaggttcaa aagaaaaaat cgcggttact tacccaggat tatttgacga
     9301 tgttcacgtt ggcggacaca ttttatttga tgatggtttg atcgatatgc aaatcattga
     9361 aaaagacgaa gcaaaccgtg aattagtcgt agaagttaaa aatgcaggta tgctaggatc
     9421 acgtaaaggg gtaaatgcac caggcgtttc tattagtttg ccaggtatta ctccaaaaga
     9481 tgctgacgat attcgttttg gtttagacaa cgatatcgac tttatcgctg caagttttgt
     9541 acgtaaagca caagacgttc tagatatccg cgaaatctta gaagaaaaag acatgactca
     9601 tgttcaaatc ttccctaaaa tcgaatctca agaaggtatc gacaacattg acgaaatcat
     9661 caaagtttct gacggtgtta tgattgcccg cggagatatg ggtgttgaaa tcccagctga
     9721 actagtacca atggttcaaa aacgcatcat caaaaaatgt aatgctgctg gtatcccagt
     9781 tatcactgct acacaaatgt tagaatcaat gcaagaaaac ccacgtccaa cacgtgcgga
     9841 agcttctgac gttgctaacg ctgtatttga tggcacagat gcaacaatgc tatctggtga
     9901 atctgcaaac ggggactacc cagttgaagc tgtttcaaca atggcacgta tcgaccaaga
     9961 agctgaaaat gcattatcag aattaggtac attccaattg aacgaattcg ataaaacaga
    10021 tgtaactgaa acaatcggtt tatctgtagc tcgtgctgca aaaaatctag gcgtgaaaac
    10081 aatcgttgct gcaactgaat caggatacac tgcaaaaatg atttcaaaat atcgtccaga
    10141 tgcagatatc ttggctgtta cttttgacga acgcacaaaa cgtggtttga tgttaaactg
    10201 gggcgtttac ccaacagttg ctgaaaaacc atcaacaact gatgaaatgt ttgaactagc
    10261 agctaaaaaa gctgttgaat taggtttcgc atcagaaggc gatttaatct tgatcactgc
    10321 tggtgtacca gttggtgaac gcggaacaac taacgtgatg aaaattcaaa tgattggttc
    10381 taaattatta gaagctcaag gcgttggaca aaaatctgtc gttgcaaatg cagttattgc
    10441 ttcatctgct gaagaagctg taaaaaaagc aaaagacgga atggttctag ttgtaccatc
    10501 aactgacaaa gaatatatgc ctgcaatcga aaaagcagct gctgttatcg ttgaagaagg
    10561 cggattgact tctcacgctg cagttgtagg aattgcacaa gatattcctg taatcgttgg
    10621 tgctaaaggc gcaacaacaa caattacaga aggtgaattg attaccgttg attcacgtcg
    10681 cggcatcgtt taccgtggcg aaacaacagc tatctaattt agcttaataa aaaataaaaa
    10741 cagctcttct tttacgaaga gctgtttttt tgtgctgtaa cgaattcatg gataacttgg
    10801 actacttgtt cttgaagagg cgtgacgatc tgctgtgttc gttttgccaa tgaaatcaag
    10861 aagcgaggct gaacttcatc tgaaagaggg aggcaaacta aatgatcttc aggatggaca
    10921 gcaatctctg ccaaaaaacc gatacccaca ttttctcgga ccatactctt taaaatcgtc
    10981 aagtcattgc tttgataaac aacttttgga tgagtatgtg tttgtttaga aagcttagtg
    11041 aatgctgcgg ggtggacata atgttcgttc aatagtacaa actgttcatc tgctatatct
    11101 ttgaaagaga cagtatcctg cttggaaaga ggatgtgtag gtggcacgac gatcatgaaa
    11161 cgtttgtcca aaagtaactc tgtttctaaa tattgatcca cgattggctg tgttgagcct
    11221 aatacagcta aatcgatcct cccttgtcgg atcatcccgt ataagtcccg agagcctcca
    11281 tcggtaatct caatttgatt catcaattca tgcttgataa agtaacttga cagtttagga
    11341 aagtattcat tgccgataat aggaggcatg ccaaaacgaa gctttttctt ttgcaactga
    11401 gtgatttcag ctcttgcagt agaaatctct tggagaatgg tttgtgcatg gagaaacagc
    11461 tgctttccag aaggcgtgac gattaattcg tgatgcgatc gatctcttgt aataagtacg
    11521 gtgtcaaact ctttttcaag acgttgtaat gcatatgtaa tagtaggttg gctcacacgg
    11581 aaataattag ccactttcgt aaaatttttt tgtttgacga tttggacata gtattcgata
    11641 tctttcgtgt tcatgaagat catccgctcc tttaatggaa taatgattat tgtaaatgat
    11701 tctgaggatg atagcaaaca gaggctttct aataaagatt tttctgtttt tttatgaact
    11761 gcaaggtgtc aagaaaattt cattgacaac atgctttttg ccctgtataa ttgcttttgt
    11821 tgcttaggag tgatgaaaaa tgaaatggtc tttattggaa ttgagaaaat accaggagac
    11881 accgttaact tttcatgaga cgctcgactt gaaagccgct ctcatgaagc gagacaattt
    11941 aatacttgat gttgcgccag tggaagtcga aggtttggtc tcagttgata agaaaggcta
    12001 tatcgttcat tacactgtgc agacgacttt gacagttcct tcaacacgtt cactagaacc
    12061 tgttgaactg cccatggatt tttctgtaga cgaagtattc atgacagaag aacaattcca
    12121 aagtagagat gaacaaatgt ctgcggatga gattttgcta attgaaggtc cattattgaa
    12181 tttagcagag tcgattgaag acaacatctt attggcgatt ccaatgcggg tattgacaaa
    12241 agaagaggaa gcagcaacag aacttccaaa aggcaatgat tgggaagttg tttccgaaga
    12301 agaataccag cgcagaaaaa tggaagaagc cgagaataaa attgatcctc gtcttgcaaa
    12361 actatctgaa ctttttgatt ctaaatcaaa agacgacgat aacaagtaag attggaatat
    12421 attgtgtcta atttagatac aagactttac aaggaggtgt agcaaaatgg cagtaccagc
    12481 tagaagaaca tcaaaagcta aaaaagcaaa acgtcgtaca cactacaaat tgacaatcaa
    12541 agggttaaac gaatgtccaa actgtggtga aatgaaaaaa agccatcacg tatgtgcaaa
    12601 ttgtggttac tatgatggta aggacgtaat gtcaaaagaa gcttaataca ttttaattag
    12661 aaaatgtaaa acctcaaggc taaccatgga gccttgaggt ttattttttt gtagtcggta
    12721 aataaaaaat atgttgttct ttagtttgca atgtagccgt gttaagctat aatgtaagag
    12781 attagggaga aatcccgtat gtaggaggag ttcatatgtc aaaacaacaa atcggcgttg
    12841 tcggtatggc cgttatgggt aaaaatctag ccttgaacat agaaagccgc ggctattcag
    12901 tggctttatt caatcgtaca ggggctaaaa caaccgctgt agtagaagaa cacccagata
    12961 aaaatttcaa agcaacatat acaatcgaag aatttgtaga atctatcgag aaaccacgtc
    13021 ggatcttgct aatggtcaaa gcaggaccgg caacagatgc aacgatccaa gagttgcttc
    13081 ctcaccttga taaaggcgat attttgattg acggaggaaa tacattcttt aaagatacga
    13141 tgcgtcgtaa tgaagagtta gccaactctg gcatcaactt tatcggtaca ggcgtttccg
    13201 gtggtgaaga aggagcttta aaaggacctt caatcatgcc tggtggacaa aaagaagcct
    13261 atgcgctagt tgcaccaatc ttagagcaaa tttctgcaaa agcagaagac gaagcaccat
    13321 gtgtcacata tattggccca aatggagccg gacattatgt gaaaatggta cacaacggta
    13381 ttgagtatgg agatatgcaa ctgattgctg aatcttatga cttaatgaag aatattttaa
    13441 acctttcagt agaagaaatg gctgatatct ttaaagaatg gaatcaaggc gaattagaca
    13501 gctatttgat agaaatcact gctgacattc tgacacgtaa agacgatgaa ggaacaggta
    13561 aaccagtagt agatgtgatc cttgatgcag ctgggaataa aggaacaggt aaatggacaa
    13621 gccaaagcgc gcttgactta ggtgtgccac ttccattgat cactgaatct gtatttgccc
    13681 gctatatttc tgcttataaa gaagaacgcg tacaagcaag caagatttta tctcgtacga
    13741 acgattttga gttcacggga gacaaaaaag aattggtcga aaaaatccga gaagcgttgt
    13801 atttcagtaa aatcatgagt tatgcccaag gatttgctca attacgtgtc gcttcaaaag
    13861 aatttgattg ggacttgcca tttggtgaaa tcgcaaaaat ctggcgcgct ggatgtatca
    13921 tccgagctcg attcttgcaa aaaattacgg atgcctatga caaaaatcct gagatcgaaa
    13981 atttattgct agatgattac ttcgttgaaa tcacgaaaaa atatcagcaa tcagtcagag
    14041 acgtcgttgc cttggctgtc caagctggcg tacctgtacc gactttctca tcagccatcg
    14101 cttacttcga ttcatacaga gcagaacgtc tgcctgcaaa tataatccaa gcccaacgcg
    14161 attactttgg cgcacatacc tatgaacgtg tcgacaaaga aggaatcttc cattactcat
    14221 ggtaccatga agaataatag tctttcggct gttaggaaat gagaaaataa aaagaggacg
    14281 aaagagctga agtcgattgt tatgagtaga cttcagctct tttttaaaat tgctatccat
    14341 gataaaatga ctatagattt ttaagcatat tatcggtata atgatagagt ttttaaaaag
    14401 ccagtagaat agaaaggata aaataataaa tgagcaatat tctaatcatt gaagatgaaa
    14461 agaacttggc acgatttgtc gagctagagt tgaagcatga agggtataac gcagaagtcc
    14521 actacaacgg ccgaactggt ttagatgcgg cattaaataa tgagtgggat gccatattgt
    14581 tggatctgat gctcccagaa ttgaacggtt tagaagtctg cagacgtgta cgccaagtca
    14641 aaaatacacc aatcattatg atgactgcaa gagattctgt gatcgatcgt gtatctggac
    14701 ttgaccacgg agcagatgac tacatcgtca aaccatttgc tatcgaagaa ttattagcac
    14761 gtcttcgtgc attgttgcgt cgaatcgata ttgaaggcga taaaaatgtg gctaaacaaa
    14821 ctactattac ttatcgcgat ttgactatcg aaaaagaaaa ccgtgtcgta cgtcgcggaa
    14881 atgaagtaat tgaactaaca aaaagagaat acgagttgct gcttacttta atggaaaacg
    14941 tgaacgtggt attagcacgt gacgttttac taaataaagt gtggggatac gaaacagaag
    15001 ttgaaacaaa cgtagtggac gtatatattc gttatcttcg caacaaaatt gacgttcccg
    15061 gggaggaaag ctatatccaa actgtacgcg gaacaggtta tgtgatgcgc tcgtgataaa
    15121 gccgaaaaaa tcggaaaaaa gagaactgaa agggccctcc ttgacaatca agtgggcctt
    15181 tgcgagttct ttcttcatat tcgttgtgtt tacaattttc gctgtcatta cctataaatc
    15241 atctatcaat ctgattgtgg caaaagaaag aaacaatgta gagcgtacga tttcagaagt
    15301 agcttctcgt ttggctaact cggatgaaga actgacatta gtaaatacgt atcgtaatct
    15361 gacgaaagcg acaaatgacg gagatgaggt cgtagatcat acttcactgg tcgaaggaaa
    15421 cttaatgaag atcgattcct ttatagctga gctagggcag ccatcaatgg atctttatgt
    15481 ttatgatttg gacgagaact tgatttttaa aacccatgaa aacagtcgaa acctgattca
    15541 gactacacgt aaagttccaa cgatcgttac actagaagga aaaactggct ttttatctat
    15601 ccagcctata tattctaaag gtacaagaga aaagatcggc tatgcgcaaa gcttctatga
    15661 attatcttct ttctatgata ttcgaaataa gctattattg actcttattg ttttagaagt
    15721 tgtttctttg atcttaagta gtattttagg tttcttttta tcttcttatt tcttaaaacc
    15781 actcaaggta ctcagagata caatggatac tatacgtaaa gatccgcaat cagatatcca
    15841 catgcctgaa atcgacacaa atgatgaatt ggcagattta gctgaaatat tcaatgagat
    15901 gcttgaccgt atgcgtttgt atattgaaca acaagagcaa ttcgtggaag acgtatctca
    15961 tgagttaagg acacctgttg caattattga agggcatctt agtctgctaa atcgttgggg
    16021 gaaagatgat cctgagatat tagaagaatc attaagcgcc agcatgcaag aaattagccg
    16081 tatgaaaagc ttagttcaag agatgctaga cttatcaaga gcagaacaag tagatgttca
    16141 ttatgcgaat gaaacaacta aaggaaaaga agtcacctat caagtttata ataatttcaa
    16201 gatattatat ccagactatg tgatcacttt agatgatgat ttgacaaaag aagtgaccct
    16261 gcaaatttat cgtaatcact ttgaacaatt aatcattatc atattggata acgcagtaaa
    16321 gtattcaact acacgaaaag aagtccatat ctctatttct tccactctga atgaatttga
    16381 gatcgcgatt caggactttg gcgaaggaat acccaaagaa gaccttaata agatcttcaa
    16441 tcgtttttat cgagtggata aagcaagagc tagaacgaag ggtggaaatg gtctggggtt
    16501 gtctatcgct aaacaactgg ttgaaaacta taaaggacgt atcttagcag aaagtgtagt
    16561 agggcaaggg acaattttta gaatctttat tcctatcgca aaggcagaag acaagtaaag
    16621 catgtgcttt acttgttttt ttatatatcc aacaaagaat aaggtacaaa atacaagcaa
    16681 gttagaaatt cagggatgtc attataaaaa aaactagcga acaattaaat agaataacat
    16741 ccgttaatta aacgtaagta gcttttagat gaacgaaaaa acaatgaaat gctgtaatat
    16801 tcgtgtttaa aaataataaa taaaaatagg gaattttcta ttgcaatttt ataaagagat
    16861 tgctatatta ttatttgttc taaatgattc ctttaaggtg tcactaaaaa ttataaaaaa
    16921 tagggttgac gcacttttga tgtcatgtta taataacgaa gtcgtttgga tgaggtagct
    16981 aataaagctt gattttatcg aaaaaaactt cgaaaaagtt gttgacaaat aacaataaag
    17041 atgatatgat aatcaagtcg ctgctaatga gtgacaaaga cgtttagaaa aaataaacaa
    17101 agaagttgtt gacattctct tctgaatctg ataagattaa agagttgttg aaacaaaaag
    17161 attagacctt tgaaaactga acaaagtaag acaaaccaaa tgtgtagggc gtcttgattc
    17221 aattcaagac aacaaacatt tttaacaagc aagcaatatg ctagcaaaca aattgagctt
    17281 aacaatcgca agattgttcg aactttttat gagagtttga tcctggctca ggacgaacac
    17341 tggcggcgtg cctaatacat gcaagtcgaa cgcttctttt tccaccggag cttgctccac
    17401 cggaaaaaga ggagtggcga acgggtgagt aacacgtggg taacctgccc atcagaaggg
    17461 gataacactt ggaaacaggt gctaataccg tataacaatc aaaaccgcat ggttttgatt
    17521 tgaaaggcgc tttcaggtgt cgctgatgga tggacccgcg gtgcattagc tagttggtga
    17581 ggtaacggct caccaaggcc acgatgcata gccgacctga gagggtgatc ggccacattg
    17641 ggactgagac acggcccaaa ctcctacggg aggcagcagt agggaatctt cggcaatgga
    17701 cgaaagtctg accgagcaac gccgcgtgag tgaagaaggt tttcggatcg taaaactctg
    17761 ttgttagaga agaacaagga tgagagtaac tgttcatccc ttgacggtat ctaaccagaa
    17821 agccacggct aactacgtgc cagcagccgc ggtaatacgt aggtggcaag cgttgtccgg
    17881 atttattggg cgtaaagcga gcgcaggcgg tttcttaagt ctgatgtgaa agcccccggc
    17941 tcaaccgggg agggtcattg gaaactggga gacttgagtg cagaagagga gagtggaatt
    18001 ccatgtgtag cggtgaaatg cgtagatata tggaggaaca ccagtggcga aggcggctct
    18061 ctggtctgta actgacgctg aggctcgaaa gcgtggggag caaacaggat tagataccct
    18121 ggtagtccac gccgtaaacg atgagtgcta agtgttggag ggtttccgcc cttcagtgct
    18181 gcagctaacg cattaagcac tccgcctggg gagtacgacc gcaaggttga aactcaaagg
    18241 aattgacggg ggcccgcaca agcggtggag catgtggttt aattcgaagc aacgcgaaga
    18301 accttaccag gtcttgacat cctttgacca ctctagagat agagcttccc cttcgggggc
    18361 aaagtgacag gtggtgcatg gttgtcgtca gctcgtgtcg tgagatg
//
LOCUS       DACECY010000054        17996 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.058, whole genome shotgun sequence.
ACCESSION   DACECY010000054 DACECY010000000
VERSION     DACECY010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.058"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(25..1521)
                     /gene="lysS"
                     /locus_tag="IWO38_002021"
     CDS             complement(25..1521)
                     /gene="lysS"
                     /locus_tag="IWO38_002021"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ0314102.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="IWO38_002022"
     CDS             complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="IWO38_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ0314103.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="IWO38_002023"
     CDS             complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="IWO38_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ0314104.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3741..5852)
                     /gene="hflB"
                     /locus_tag="IWO38_002024"
     CDS             complement(3741..5852)
                     /gene="hflB"
                     /locus_tag="IWO38_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ0314105.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="IWO38_002025"
     CDS             complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="IWO38_002025"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ0314106.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="IWO38_002026"
     CDS             complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="IWO38_002026"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ0314107.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8052..8522)
                     /locus_tag="IWO38_002027"
     CDS             complement(8052..8522)
                     /locus_tag="IWO38_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ0314108.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8568..8993)
                     /locus_tag="IWO38_002028"
     CDS             complement(8568..8993)
                     /locus_tag="IWO38_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ0314109.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9060..9329)
                     /locus_tag="IWO38_002029"
     CDS             complement(9060..9329)
                     /locus_tag="IWO38_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ0314110.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9340..10935)
                     /locus_tag="IWO38_002030"
     CDS             complement(9340..10935)
                     /locus_tag="IWO38_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0314111.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="IWO38_002031"
     CDS             complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="IWO38_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ0314112.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14488..15048)
                     /locus_tag="IWO38_002032"
     CDS             complement(14488..15048)
                     /locus_tag="IWO38_002032"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ0314113.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15401..16375
                     /locus_tag="IWO38_002033"
     CDS             15401..16375
                     /locus_tag="IWO38_002033"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ0314114.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16545..17945
                     /locus_tag="IWO38_002034"
     CDS             16545..17945
                     /locus_tag="IWO38_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ0314115.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
BASE COUNT         4891 a         3978 c         3248 g         5879 t
ORIGIN      
        1 attccttaga cagcttttat tcgattatct cattgttggg aatagcaaaa catcccgaat
       61 tgattgtgca tcagttagca acattactaa tctgtcgatc ccgatgccta gaccaccagt
      121 tggcggcata ccgtattcta aggcttccaa gaagtcttcg tctacaccgt gagcttcgtc
      181 atttcctagt tcacgttctt tttcttgcgc ttcgaatctt tctctttgat caattggatc
      241 gttcagttct gtaaaggcat tagcaaattc gcgccccact atgaatagtt cgaatcgatc
      301 tgtgaatcga ggatcttctg gatttttctt cgctaaaggc gaaacttcga ctggatgtcc
      361 gtatacaaac gtcggttgtg tcaatgtttc ttcaacaaat gtttcaaaga attcattgat
      421 aacatgtccg acacccatag cttcggttac ttctacattg tgtttttcgg ctaaagctaa
      481 tgcttcttcc aatgtcattt cttgccagaa gtctacacca gtttgttctt tgattgcatc
      541 taccatatgg atgcgtttaa agtcactttc taagtcgaca gcttgtccat catatgtgat
      601 ttttgctgtt cctagtactt tttcagctgc gttacggata ataccttctg tcagattcat
      661 tacatcattg aaatcagtat atgctgtgta tgcttctaac atcgtaaatt ctggattatg
      721 tgtagtatca attccttcat tacggaaaac gcggccgatt tcataaactt tttccattcc
      781 gccaacaatt agtcgtttta aatgaagttc tagcgcaata cgcaaatata aatcaatgtc
      841 cagtgcatta tgatgcgtga taaatgggcg tgcggcagct ccacctgcct cattgtgcaa
      901 tactggtgtt tctacttcaa tatagccatt gccatctaaa tagcgacgga tttcgctgat
      961 aatttggctt cgtttcataa atcggtcaaa gctttcgcga ttactgatta aatccaagta
     1021 gcgttgtctg tagcgttgtt caatattcgt taagccatgg tatttatctg gcagtggacg
     1081 aagtgctttt gacaaaatcg tgatttctga agctttgata gaaacttcgc ctgtgtctgt
     1141 tttcatgatt tgtccagtta caccaaagaa atctccaagg tctgcatgtt tgaatacttc
     1201 ataggcctca tcacctactt ggtcttttct tacataaatt tgaatttggc cttcacgatc
     1261 ttgtagatgc gcaaatccag ttttcccttt tcctcggcga gtcatcatac gaccagctac
     1321 actagcggtc aatcccattt ctgctagttc ttcttttgtt cttggatcaa atagttcatg
     1381 caattcttct gagttgtgcg tgcgttcaaa tcgttttcca aatggatcga ttccatcttc
     1441 acgcaattgt tccatttttt gacgacgtac aagcatttga tcatttaaat cttctacttg
     1501 atgttgttgt tcctgtgtca caaaagttcc tccattctca tgttaatctc tttcctataa
     1561 tgccaatgaa acacaaaaaa agcaagccga acttggaatt tcggcttgaa aattcacgaa
     1621 aaacttagaa atcacaacgt ttgtttcaat agtacttttt ccgctttttc tacaaattga
     1681 tccagcaggt cattgatttc ttgctgtttt tctgtctgat tgattgctac tttcactttc
     1741 gctgctcgag gaatcccttt taaataataa gccgcatgtg ttctgaattc tctacaagct
     1801 actttttctc ctttgagatc tgctaagcgt tgtaaatgaa gtttagctgt agcaattttt
     1861 tcggctggtg ttggttcagg aatcagttct cctgtttcga gataatgctg tgtgcggtga
     1921 atcatccatg gattgcccag cgctgcacga ccaatcatca caccgtctgc accgacatag
     1981 tcaagcatcc attttgcatc ttctggcgtt ttgacatccc cgttccccat aaatgggatc
     2041 gtcaaatgtt ttttcacttc tttcaatact tcccagtccg cttttccttc atacatctga
     2101 acccgtgtac gtccatgcat agcgatggca cttgctccag ctgattgtgc tgctaatgca
     2161 ttttctacag caaagacgtg ctcttcatcc caaccaatac gcatcttgat tgttactggg
     2221 atatttactg ctgaagaaac agcttggacc atttcgtata ctttctcagg atctaaaagc
     2281 cattttgctc ctgcttctgc tttgattact ttatttactg gacagcccat attgatatct
     2341 attatatcgg cagctgtgtt ttcttcgaca aattgagcag cttcaactag actatcttta
     2401 tttccgccaa atatctgtaa acttaatgga tgttctgttt cttcaatgtg gagcatctct
     2461 aaagtttttt tgtttctgaa atgaatacct tggtcactga tcatttcaca tacgactaag
     2521 cccgcaccaa attctttgac agtgactcga aatgctgaat ttgttattcc agccatcggt
     2581 gcaacaacga cacgattagg gatttccaca ttgccaattt tccatgtgtg ttccacttaa
     2641 atccctccta aggcttcttt cttcaatcca tctaattctt cttcgctata cttgtatttg
     2701 ttattacaga aagaacaaac cgcttctgca ccgtgatctt cgtctatcat tgcttggatt
     2761 tcttctgcac ctaacgcgat aattgcttct gcaaattttt cttttgaaca gtcacatttg
     2821 aattgaacag gcattttttc caaaatgttc aattcttctg taccaagcaa tcctttcaat
     2881 atttcttctg gtgtctgtcc ttcatctaag agggtagaga ttcttggtgt cgtttttaat
     2941 tgttcttcga tcttgctgat tgtttcttca tcggcacctg gcatgatttg gatcatgaat
     3001 cctcccgccg tcttgatgct gtcgtcagta ttgaccaata cactcagtcc aacagctgaa
     3061 gggatttgtt ctgagatagc aagatagtaa gtaaaatctt cagcgatttc tccagaaaca
     3121 atcggcgttt gcccagaaaa aggttctttt aatcctaagt cttttgtgac agtaaacatt
     3181 ccttgtgttc caactgctcc ccgcacatcg attttacctt cactatttag aggaagactg
     3241 agatgcggat ttttgatgta acctttcaca tctccctttc catttgcatc tactacaatt
     3301 cctgttgctg gaccatcgcc ttggattcga acagttagtt tgtcttctcc tttgagcgtc
     3361 gcaccaagta gcagactacc gaccaatgtc cgtcccaaag cagctgagga ggtgtgccaa
     3421 gtatcatgac gtctttgtgc ctccgctacg gtatttgttg cttgaacggc gtatgctcgg
     3481 acgaatccgt cgtgagcaag tgccttaacc aaataatctt tcatttgtta tgtactcctt
     3541 tttccgtttt taaactcata gtgaatcata ctagcaacta ctgctttttt caactttgga
     3601 atgcttcttt taaaaataat tcagaatcat ctagaaaaca ggacatcacc ctatttacaa
     3661 cttaaataaa gaaatctccc gaaaaccggt ttttgttccg gttttcggga gtcatatcag
     3721 atcaattgac ctatattttt ttatttgtat tgatcgtctg aatgattatc ttctgattgt
     3781 tgagaatctg aatcatgttc tgctttattt tcctcattca gttcatgttt tgcttcttca
     3841 aaatcacgtt tttcttccgc ttgtttttca gcgtcttttt cttccaaagc acgttttgct
     3901 tcttcaaaag ttttcgcttc tttttcgctt ggataatcat ttgactcagt accttcaggc
     3961 atcacaccgt gttcaaacaa tgacttgatt gctttggcat ccaatgtttc gaattccaaa
     4021 agtttttcag caatcagttt atgctgtgca cgatgagctt cgatgatttc tcgagcttta
     4081 tcatgagctt ccatcaagat ttttcgtact tcttgatcaa tctcgaaagc gacttgttcc
     4141 gagtaagctt ttgtttgacc atagtcgcgg ccaacaaaca cttgatggtt tccttcatat
     4201 tgaacaggac ctaaacgatc actcattcca tattctgtta ccatgcttcg tgctagagct
     4261 gtcgcttgct caaagtcatt ggaagcacca gttgattgga cattgaagat gatttcttca
     4321 gctgtacgtc caccaagtaa gcctacgatt tgttcaaaca tatcttcttt cgtcatcaag
     4381 aactgatctt cttttggtaa agcgatcata tatccgccgg cgcgtccgcg agggatgatc
     4441 gttactttgt ggacgacccg agcgcgactc aatactaatc cgacaatcgt atgaccagct
     4501 tcgtgataag caaccatttc gcgttctttt ttgctgatta cgcgatcttt tttagctgga
     4561 cctgcaatca cgcgatcttc cgcttcatcg atatcagaag catcgatttt ctttttattt
     4621 cttctagcag ctactaacgc tgcttcattc aagacatttt ctaaatctgc tccggcaaaa
     4681 cctggtgtct gttgagccac aactttcaag tcaacgtcat ctgccatcgg tttattccgt
     4741 gcatggactc gcaagattgc ttcacgacct tttacatctg gacgtccaac taaaatctgg
     4801 cggtcaaaac ggcctggacg tagtaacgct gggtctagta catcagaacg gttggttgca
     4861 gcaatgacga ttactccttc atttccgtcg aagccatcca tttctacaag caattggttc
     4921 aatgtttgtt cgcgttcatc atgtccgccg cccataccgg caccacgttg acgacctacc
     4981 gcatcaattt catcaatgaa aatgatagct ggtgcgtttt tcttcgctgt ttcaaacaaa
     5041 tcacgaacac gacttgctcc aacaccgaca aacatttcta cgaaatcaga acccgagatc
     5101 gagtaaaacg gtactccagc ttctccggca acagcttttg ctaataaggt tttacctgtt
     5161 cctggaggtc cttctaaaag tacacctgcc gggatacggg cacctaattc aacaaatcgg
     5221 cgaggatctt tcagaaattc aacaacttca actagttctt gtttttcttc ttccgcaccc
     5281 gctacatcag aaaaacgtac gcgattagct tttttgtctg cttctttggc ttttgattta
     5341 ccaaagttca tgacacggcc gccaccgcca ccgccgccgc cttgttgtcc catcatcatg
     5401 taaaagaaaa agatgatgat tacgattggc aagaagctaa gcaataggga aacccatata
     5461 ccgctgcttg attcttcttt tactgttgta gcaactttgt tttcttttgc cagttctgtt
     5521 acttggctca atgttgtatc atttggcaaa atgattgttg tgaaatgggt agtagtcgta
     5581 tctgttgaac ctaagatagc taacccaccg ctgttagcga cggtctgctg ttctttgtat
     5641 tcaccagtga tcttatatac accatttgca ggctgcactt caaattcttt gatcttgcct
     5701 gcttgtagtt gttctgtgaa ttttgagtac tcgatatctg acgatcgctg gccgttatta
     5761 ccaaaaatga aataaacgac cattaccatc gctaagatta cgaggacata atacaaggca
     5821 tttttcatca tgccgttgtt ctttttgttc atagttgccc tccctaagcg tcaatttaat
     5881 ttaacagttt tttatcaatc ctgaccattc tattgtacca taatttaatt gagatacagc
     5941 attaatttga ttgataaaca ctaggtttca atacaccgat gtacggaagg ttacgatatg
     6001 cttctgcata atccaagcca taaccgacca caaattcatt tggtacgttg aagccaacat
     6061 aatctgcttc gatatttact actcttcctt caggtttgtc aagtagtgta acaattttga
     6121 ctgattttgc tttgcggtat ttgaaaagat ccactaaata agcaagtgtt cttccgctgt
     6181 caatgatatc ttcaacgatc aacagatcgc gaccttctac attcgtatct aagtctttga
     6241 cgatttttac ttcccctgac gagactgttg cattcccgta gcttgaaaca tccatgaaat
     6301 ccaattccaa atacgtatcg atagaacgaa cgatatctgc catgaatggt accgctcctt
     6361 ttaagacacc tactactagg ggattggtgt tttggtaaat ttctgttaac tctttgccta
     6421 gttctttgca acgaacttgt atctcttctt gtgagatcaa aattctctca atatcctttt
     6481 ccaacatcag gttctccttc caattgcttc tttttgatat ttgtaaagaa gtatgtagtg
     6541 tattttatca gtttcttcac gaatactcaa atatgaaaag gcaaaaggta tcaagctata
     6601 tatatttctt ttggcatctg taatgatcca agcatgctgc ctttgtgaat taggtacttt
     6661 ctgatcgata aaatatctag atactttttt tgtgagattc tctgttaatt taatgcggtc
     6721 tcctgctttt cttttttcga catacagttg cacatcagca ggaatccaaa tgtcatgaga
     6781 aaatagagac catgaatctg ttgctttgat gtcccctgca ttttcaggtt tgaccagacc
     6841 aatccattct gtttcattca aatatattcc ttgttctgga atcagcaaca agcgctggtc
     6901 ttctgtctgg ctagttgctt gtttttcttt tttagagagt acaacaaaat gatactctct
     6961 tttcagtatc caattattgc caatactgag ttgccattgg ctttttttct gttgccactg
     7021 gtctaaaatc gactccattt gttgttcttt caaaataatt cctgtttttg agaatgcttt
     7081 gtcgaaaaag gagtaaaaaa aatagtatcg ttcagcatct gtcatttctt caatcatttc
     7141 tgcagaaaat cgaaattgtt cctcccactg ttcgacttcc ttttcgatca actgtcccat
     7201 atatttttga atgacttggt ctgcccattg gatctgttgc gaaaattgct ggaaatgtct
     7261 caacgcctgt gtattttctt gtttcaattg aggaactacc aaatgacgaa gccgatttct
     7321 ctggatatct aacaattggt ttgtctgatc ctcaaagtaa acaagctgcg attccgctgc
     7381 ataagtatat aggtcttctt tgctaaaact cagcaacgga cgaatcagtc tgcccgtagc
     7441 aaatggttgt acttctttga ttccagaaaa agtatttaac tgtcccccac gaacgatttt
     7501 cattaacacc gtctcgattt gatcatctcc gtgatgagca gtcatcaacg tgtcatattg
     7561 tccttcttcc atcacgcgag tgaattcttg ataacgaaag ttacgagctt ctgtttctat
     7621 ggcggatttg gctggatctt cccaaatact tacatataaa gggagattct tctcttcaca
     7681 atatgagcga agataggcag cttcttctgc tgattcttct cttaattggt gattgatgtg
     7741 gacgatacct aaagaaaaac cgatttggcg ttgcacttgt aaaagtaaat ggagcaaaac
     7801 catcgaatca acaccacctg ataccgctgc taaaattctc gatccttgtt gccagaatcc
     7861 ttgttcattc ccgtgccgaa taaatgactg ctgtaatagc ggtgcttttt tcccgccttt
     7921 ttcaacgttc atcctttcca caccctttct tcctaaaaaa aagagagcgg gaaaacgtct
     7981 aaaacgacaa ttaacctgcc ctctgttgat actatgaccc aaatagtcgc actatcatta
     8041 tttgcggctt tttagctgcg acgtccgcca cggccaccgc gctttccttc tgtgtttcgc
     8101 ttcaatgaag ataagcggtc atcgctatct ttcaaaaatg aactcatcaa tgaatcgaag
     8161 tcttcttttt gagctgaagg ttgactgcga gaaaaagatt ttttctgtgc tggacgattt
     8221 cggttgtcac ggggatcttt ttgttgatat tttttaggac ggctttcttc tccagattca
     8281 gcaggttgtt cctgcgcttt ccggatagat agtccgattt ttccatcatc atttactgaa
     8341 ataactttaa ctgttacttc atctcctaca cttaaaacat catggatgtc ttttacatag
     8401 ccatttgaca cttcgctaat atgaactaga cccgtttttc cagcacctag atcaataaac
     8461 gcaccaaaat ttgtgattcc tgatactttt ccttgcagtt ttgctcctac ctcgattgac
     8521 ataaaaaaaa tgttcctcct aattatttcc cttttttata ttcctgctta ttttgcagtt
     8581 gtagaactag ttgattgatc tgtttcattt gtcgagctgt ctgtatttga ttctaatata
     8641 ggataaactt tttcaccgtc ttttgagtaa aagaatttgc ttctggcaag tttagctaca
     8701 tactcatcat ctttcagcaa tgaaacatct tggtgcagct gatcaacgtc tgcgttgact
     8761 tctttcattt cagtatttgc ttcgactctc aattcgttca attgatgcat cctttgcatg
     8821 tcatggaaaa tctgcacacc tattatcgcg aaaatgacca aagcaccaac aaaaattgca
     8881 gccagtctgc gtcttcgaaa aatcagttga cgttgttgtt gctgaaattc agcatacttt
     8941 ttctttgcat attccgtatc tagcgcagcg attttattgc tttttttcgt cacttggatt
     9001 cgctccaatc tttttgattg tctttttcat tataccaata tcgtgaaagc ttgtctaatc
     9061 tatttctcta gtgtttctgt gcgattttca gaaataattt cgtacatttt ttgtgcatct
     9121 tcttttttgg ttgattcatg taattccgta attttcactt ccaacgtttt gttgccaaac
     9181 tggactttca aaatatcgcc tactttgaca tcactggaag atttcgccaa tttaccgttg
     9241 atttgaattc ttcctttatc agctacttct tttgctactg atcgtctttt gatgattcgg
     9301 gaaattttta aaaatttatc taatcgcata tcttattcct cactttttaa tttttttatt
     9361 taatcgcagg attttcttac caaaaggcaa cattagccat tctcgtactg taaacagacg
     9421 aagacgaatc actgcataaa tgaacacaga agccccgatc ccaactccta tcacgctgac
     9481 aaaaaaagca gtggaacgat gagttacagg accaaatact atattcaata caccataata
     9541 cagcagcagt actactgtca tacaaagaag gctgaacatc aacttccgtc caaaatgccg
     9601 ttctttccaa aaagcattta gctcttttgt ttcaattcga acaaaatacc aaagcgtcac
     9661 acctaatcct aaaagggtag acaggcttgc acctgctgtt cctaataaac cggtcagaaa
     9721 agaagtagtc aatcctttta ccaatagtcc ccatcctgcg ccttttaaag aagggcgaaa
     9781 cgagttcttg ctttgagcaa tactttgata agcttggatg actgcagtga aagcaatgga
     9841 aaaaacaaat aatacaagcg ctgcatttcc cgcataatct ttaaacaacg cataattgat
     9901 ataagggagc agcaaggcta atcccaaaga cgcagctaat gctaaagcgg tcgtcaatct
     9961 aagatagatt ttggctgtct gtaaaaactg acgatagatc cggttggtca aatgacgagt
    10021 caacgcaggc aggaatgtcg cacttaatgc agtggcaatc actaaaccta attgtactaa
    10081 aggctgacct cgatcataga ccccttttgc tatcttcgca ccgtattctg acagtccgta
    10141 aacttccaaa gcatttttta caaagaaaga gtcgatcagc tggaaaaaga taagcaatcc
    10201 actataaatc gaaaccagtc cgccttctac cagaaaacgc cggatcaagg gctttgacgg
    10261 ctgtttgaac aatggaaacc gtcggaagct taacgctcct ccatgaatct tttgttcata
    10321 gtagtgaagg atcccatatg cgcataaacc gccaaaaaca gctccactca ttgctgccgt
    10381 ccctgtttgg tacaccgtcc atccaagctt tctaaaagca aacgcactac agacaatcac
    10441 aaaaacacga acgaactgct caactacttg agaaacagca gttggttcca tcaagaatct
    10501 tccttggaaa ttccctcgat aaaaagatag accaggcatc agcaaaaagg taaaggaaac
    10561 tacttggatc agcggtttca attgtatgtc tcccatcatc caagcaatcc agctgctaaa
    10621 tgcaaagaca aacaaccaca aaaagacacc tgtccaaaat actaagggat aaagattttg
    10681 caaagcttgc tttcgctcga aaggattctt tctttctgca acgtactttg atatgaactg
    10741 cggaagaccg gataaagcta aagtcattgc tatcccataa attggataaa cttgctgata
    10801 aacataaaaa ccttcgtcgc cgactaagtt ttggagggga attcggtaaa gggcacttaa
    10861 tacttttgct atgaaagaag caatcgtcaa tacaaatgct ccttgcatga cgcgccgcat
    10921 ttctttatga tccaaatcat tgctccttcc tacgctgaca gatatttttg ctcacgcaac
    10981 gctttgacaa acttttggat ttcctgaagc catactgctt cttccatttg gttggggatc
    11041 gtcaagcgta tcaccatctg ttctttttca acgcctagac tggcttttag ttttgtcgct
    11101 gtcagtgctt cgaataactg ttccacacta tagcgttttg ttccaacttt acttagagta
    11161 aagactatat gcgcctcctg tttccttatc gtttctagga gcgctcgatc gccatccatt
    11221 ttgatctgcc caattgtcaa gagatgtgct acttcttctg gatattctcc aaaacggtcg
    11281 agcagatcat cttctagttc atctagcatt tcttgcgaat caagttcacg aattcgttta
    11341 tagatctcga ttttctgccg ttgatctgcc acgtatgttt caggtagata cgcatcgaca
    11401 ccgaggtcga tctcaacagt ggtcttctcc acttgactat tctttccttg tttacgagaa
    11461 acagcttcgc ttagcatttg agaatacata tcgaaaccga ctgcatcaat aaatccatgc
    11521 tgctgagcac ctaagagatt acctgcaccg cgaatggaaa gatcccgcat cgcaatcttg
    11581 aatccagatc ccagttctgt aaaatctttg attgcctgaa gacgtttttc actgacttcg
    11641 ttaaggatct tctgctgctc atacataaag taagcatatg cgacacggct gcttcttcct
    11701 acccgccctc gcaattggta taacgtggat aatcccatat aatcagcgtt ctcaacgaac
    11761 aacgtatttg cgttcgggat atctactcct gtttcaatga tggttgtagt gacaagaaca
    11821 tcatattgac cttcaataaa ttcaaaaagc gtattttcta attgcgcctc tgtcatttgc
    11881 ccatgagcat aaccgatccg tgcttccgga acgagttctt ggatttcttc tactttttgt
    11941 tcgatcgttt ctacccgatt atataaatag aatacttgtc cacctcgtcc catttctcga
    12001 tggattgctt cgcgaatagc cccaggattt ttttccataa catacgtttg gatcggataa
    12061 cgattcgcag gcggtgtttc gattactgaa agatcccgca cgcctaacat cgacatatgc
    12121 aaagttctcg gaatcggtgt tgcagtcaga gtcaaaacat ccacttgtgc acgtaactgt
    12181 ttcaatcgtt ccttatgttt tacaccaaaa cgttgttctt cgtcaatgat cagtagaccc
    12241 aaatcgctaa attcaatatc ttttgacaaa attcggtggg ttcctacaac aatatcaact
    12301 tgacctgtac gcagttgttc gatggtttct ttttgttgct ttcttgttct gaaacgactt
    12361 aatagcccga tatttacggg gaaaccttca aatctttcca gcatcgtttc atagtgctgc
    12421 tgtgccaaga tcgttgttgg aactaaaaag gctacttgtt tgctttcctt tatcgcttta
    12481 aaggccgctc gaagcgctac ttctgttttc ccataaccaa catcacctac gagtagtcga
    12541 tccattggtt tttctttttc catatcccgt ttgatctccg ctgcacttct caactggtca
    12601 tcagtttcag agtaggggaa ggcattttcg aattcttttt ggtaggcgtc atcaggtcca
    12661 aaagcatatc ctttttctga ctctcttgcg gcatataatt tgattagatc gtcagcgata
    12721 tcctcgattt ttgaagagac ttttcgcttg gttttcgtcc attcacttcc gcctagctta
    12781 ttgatgcgag gcgctttggc ttcagaggcg acatattttt ggatcagatt cagttgagta
    12841 acaggaataa acagtttgtc gtcgttttga tacaaaatag tcatatagtc ttggtgaacg
    12901 ccatctactt ctagcgtttc catcccaatg tatttcccga taccatgatt ggcatggaca
    12961 acgtaatctc ctgcttttaa ctcattatag cttttcaatc gctctgcgtt tgttactgtt
    13021 tgacgtctcg tttgtttttt cgtcgttttt tggaaaattt ctttttctgt gatagctact
    13081 attttttctt gaggaagttc aaaaccagcc tgcaaagctc cttcaactaa ttgtacctgt
    13141 cctctgatca atgcattttt ttctgtttct actactaata tgtcttcatc tcgaagcatt
    13201 tgttctgctt tgcgtattct gtcctttgtc ggaataaaga tcacaacagt ctgtttttgc
    13261 ttgcgccaac ggtccatttc tgttttcagc aatggcattt gtccgaagaa ctgttgcatc
    13321 gtccgatatt gaaaattatg gattgcttgg aaacgaagat ttcccatccc tttttggaag
    13381 agtgagaaaa aggttgtggt gaacttttcc ttctgcacga ttttttgtat atctgctgaa
    13441 aatgtctgct cagaaaacac ccgcaattcg ctgatttttt gagtctgcca ttctgccgct
    13501 tccctttcga tttcccgatt tgtttccatg attcgctgat aatcatcaac gactagtaaa
    13561 ctatttgaag ggaaataatc caagatcgtc acagattctt ggtaaagaag atctgagtag
    13621 aattttgctt cttctgtcgg gacacctgct tgccagcttg aagtcagctg accgaagtaa
    13681 tcttgcaaaa actcgcggtc tgcttcttct gtggtcgcag caagccgttt ttctagcatt
    13741 ttttgcattt gctctattcc atgttctaga tcctctggtc cgtagacttg ttcactcgtt
    13801 ggggaaaccc agatttcttc cgttttttcg attgagcgtt gtgtttctgc ttcaaaatac
    13861 cgcatagaat ctatctctac atcaaatagt tctacacgaa caggataggt tgtcgttaac
    13921 gggtaaatat cgataatgct tcctcgaata ctgaattctc ccggttttgc gaccatctct
    13981 tctcgtacat agcccatcaa agtcaattgg cgaggaagat tttccacgtc tatctcatca
    14041 ccaatatgcc aattcaattg cgcctgtagc caagttttct tattcggcag tcgtttgtgc
    14101 aaagatgcga ctggcaacaa atagatacct gctttttttt cttgaagtgc gttaagcgtt
    14161 gcaactctct cgcctcgcgc ttctggagaa gaaaaggcca tttcagcaga aagcacttca
    14221 tccactggaa ataaatggat ctgatcttgc ggaatcacat gttgcagatc ttctgctaac
    14281 tgattcgcat aatacaaatt tggcatcaga ataatcatgt ttttatttgt tttcttaaac
    14341 gctcgagcaa atagaagtgt tttcgctgag cccgctaatc ctgtgattag ttgtctaggc
    14401 attttttctt ctaaattgct gacccattct tttacttgcg gatcttttcc gactaattga
    14461 ataatatcca ttccttttcc tccttcctca attgaatcga ttcatcgcat ccacgaacga
    14521 atgcccttct atgagatatt cgactgcttt aacagattga cacatgcttt cttctatcaa
    14581 aggctgattt tctttgctga aaggactaag cacatgctgg actactgttt ttttcccttc
    14641 tggcctgccg attcccacct tgattcgatc aaagacattc gtattcaaat gggaaataat
    14701 acttttgata ccgttatgcc cgccggcact tcccttttga cggagccgga tttttcccac
    14761 tgccagatcc aaatcatcgt agattacgac taattcctct ggataaatcc caaaataagt
    14821 cattagtggg ccgactgcac gaccagattc attcatgaat gtttgtggtt tgactaaaag
    14881 gatcttttct ccattatgaa aaaattcggc aacttctgct tcgaacggat tttttttgaa
    14941 agtagcttga tgctcgcgtg caattttatc cacgaccata aagccgatat tgtgttttgt
    15001 atattggtat ttggtacctg ggtttcctaa cccgacgatc attttcattt ttgttttcca
    15061 cctaattcta aaaagcagca caaaaggctg gactgctaat tagcggtcca gtccatcgag
    15121 cttttttctt ttttatactt gtcaatgcat agtataacac aaaacaaggc gtttcggcta
    15181 aaagagaatc gttaataaat gataaagaaa ttaaaaattt cttcaaagtt taatgaaata
    15241 attgatatat aaaaatagtg aaataacgca catgtaagct tttacatata atacaacctt
    15301 gttatctgtt atattttata gccaaatagc gtgacatggg gcttgagaaa tgttagcata
    15361 agcatgggaa aaaacaaatc aatggaagga ttgatactaa atgactgcag ctgcagaaaa
    15421 aaaagatcat caaaaagtta ttcttgttgg agacggcgcc gtaggttcta gctatgcctt
    15481 tgctctagtt actcaaaata tcgctcaaga agtaggtatt gtggatatca atactgctaa
    15541 aactgaagga gacgcaattg acttgtctca cgctttggca tttacttcac ctaaaaaaat
    15601 ctattctgct acttacgcag acgcacatga tgctgactta gttgttatta cagctggcgc
    15661 accacaaaaa ccaggtgaaa ctcgtttaga cttagtaaac aaaaacctaa aaatcaaccg
    15721 cgatgttgtc acacaaatcg ttgaatcagg atttaacggt atctttttag ttgccgctaa
    15781 cccagtagat attttgactt actcaacttg gaaattctca ggtttcccta aagaacgcgt
    15841 aatcggttca ggtacctctc ttgactcagc tcgtttccgt caagcacttg cggaattagt
    15901 tgacgttgat gcacgtaacg tccatgctta catcttaggt gaacacggag attcagaatt
    15961 cccagtttgg tcacatgcaa atgttgccgg cttgcaaatc tatgaatggg taaaaaacaa
    16021 cccagaaatc gatgaagaag ctatggttaa cctattcttt ggcgtacgtg acgctgctta
    16081 cacaattatc gagaaaaaag gcgctacttt ctatggtatc gcagtagcat tagcacgtat
    16141 cactcgtgcc atcttagatg atgaaaatgc tgttcttcct ttatcagtat tcatgaatgg
    16201 cgaatatgga ttgaacgaca tctacattgg tgcacctgct gttatcaacc gtcaaggtat
    16261 ccaaaaagta atcgaaattc ctttaagtga cagcgagcaa gaccgtatgg ctgcttctgc
    16321 taagcaatta aaagaaattc tagatgaagc ttttgctaaa ctagacgctg aataatcttt
    16381 gaacatgacg gaaaacatct attcggtgtt ttccgttttt ttattttatc acccttatgg
    16441 tttctaaatt ttttcataaa gtagagataa ccttcgtctt ttcctctaaa ttatgctatc
    16501 atgagaccgt tccaccctaa acttttactt aaggagttgt cgatatgaca agatcttcgc
    16561 atcgaaaaaa atcgaaacat gttgctttgc tttcagttct tggtgttctc ttagtgctga
    16621 tcagctttta tacctatcgc agtacatact acacaaagca ttttttgccg aatacagaaa
    16681 taaatggaat aaatgtcagc aatctaacag tcgaaaaagc aaatgaaaaa ttaaaagaag
    16741 cttattctga taaaaaactg gctattaaag aagatggaaa agtctggcag gaagtaccaa
    16801 aatccgagct cggctacaaa gatgatttca cttcggagct ttctccgatc ctaaatgaac
    16861 aaaacggatg gacttgggga atgacttatg tctcagcggc agaaaaacaa gaaatagatc
    16921 ctgtcagtca aaaccaccaa aaactagaca ctaccgttca gacattgaca actaagctga
    16981 ctgatttgaa caaagaccgg acacctacta ctgatgcaac gatcgaaaaa gcaggtgact
    17041 ctttcgttat caagcctgaa gtcaatggaa atacgatcga cgtcgatgct gcggtcaaac
    17101 aattaaaaag tgcggtaaac tctggtaaag atacaattga attgactgaa ttcaaagaaa
    17161 aaccaaaagt aacatctgaa gacagctcac tgaaagaaca actcgcatcc atgaatgcta
    17221 tcgcaaatgt aaaagctact tacagtataa acggtgagac tttccagatc ccatcaagtg
    17281 atattatgag ttggctgacc tacaatgatg gaaaagtcga tttagataca gaacaagtac
    17341 gtcaatatgt aacagattta ggcacaaagt ataacacaag tacgaatgac acaaaattta
    17401 aaagtacaaa aagaggagaa gttacggtcc cagttggcac ttacagttgg acgatccaaa
    17461 cagacagtga gacagaagca ttgaaaaaag cgattctggc aggccaagat tttactcgtt
    17521 cacccattgt gcaaggtggc acaaccgctg atcatccatt gatcgaagat acgtatatcg
    17581 aagttgatct ggagaaccag catatgtggt attacaaaga cggaaaagtg gcccttgaaa
    17641 cagatatcgt ttccggaaag ccaactacac ctacacctgc tggagtcttc tatgtttgga
    17701 ataaagaaga agatgcaact ttaaaaggaa caaatgacga tggaacacct tacgagagtc
    17761 cggtaaacta ctggatgccg attgactgga caggtgttgg tatccacgat tcagactggc
    17821 aacctgaata cggtggagac ttatggaaaa cccgcggttc acacggctgc atcaacaccc
    17881 caccaagtgt aatgaaggaa ttattcggga tggtcgaaaa aggaacgcct gtattggtct
    17941 tctaacaaaa ataatatata aaaagaaagc acccgtacga ttggtacagg tgcttt
//
LOCUS       DACECY010000055        17714 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.059, whole genome shotgun sequence.
ACCESSION   DACECY010000055 DACECY010000000
VERSION     DACECY010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.059"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            3..815
                     /locus_tag="IWO38_002035"
     CDS             3..815
                     /locus_tag="IWO38_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ0314116.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            812..1612
                     /locus_tag="IWO38_002036"
     CDS             812..1612
                     /locus_tag="IWO38_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ0314117.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1773..3041
                     /gene="rseP"
                     /locus_tag="IWO38_002037"
     CDS             1773..3041
                     /gene="rseP"
                     /locus_tag="IWO38_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ0314118.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3109..4818
                     /locus_tag="IWO38_002038"
     CDS             3109..4818
                     /locus_tag="IWO38_002038"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ0314119.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5030..9382
                     /locus_tag="IWO38_002039"
     CDS             5030..9382
                     /locus_tag="IWO38_002039"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ0314120.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9526..9999
                     /gene="rimP"
                     /locus_tag="IWO38_002040"
     CDS             9526..9999
                     /gene="rimP"
                     /locus_tag="IWO38_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ0314121.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10023..11198
                     /gene="nusA"
                     /locus_tag="IWO38_002041"
     CDS             10023..11198
                     /gene="nusA"
                     /locus_tag="IWO38_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ0314122.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11220..11513
                     /locus_tag="IWO38_002042"
     CDS             11220..11513
                     /locus_tag="IWO38_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ0314123.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11510..11821
                     /locus_tag="IWO38_002043"
     CDS             11510..11821
                     /locus_tag="IWO38_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ0314124.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11834..14140
                     /gene="infB"
                     /locus_tag="IWO38_002044"
     CDS             11834..14140
                     /gene="infB"
                     /locus_tag="IWO38_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ0314125.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14167..14514
                     /gene="rbfA"
                     /locus_tag="IWO38_002045"
     CDS             14167..14514
                     /gene="rbfA"
                     /locus_tag="IWO38_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ0314126.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14622..14843)
                     /locus_tag="IWO38_002046"
     CDS             complement(14622..14843)
                     /locus_tag="IWO38_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0314127.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15291..17654
                     /locus_tag="IWO38_002047"
     CDS             15291..17654
                     /locus_tag="IWO38_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAQ0314128.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWELFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6035 a         3146 c         3903 g         4630 t
ORIGIN      
        1 gaatgttacg tttttttccg caaaaaaaca aatatattca agaaaaaagc gaatttcaat
       61 ttaataccga tggaccaatt cctcagcaca ttgccatcat catggacggc aatggtcgtt
      121 gggcgcaaaa ccgtcgtttg ccacgagttg ctggccataa agaaggcatg gaaacagtaa
      181 agaaagtcac aaaaaaagca tcaaggcttg gagtgaaagt gttgacgctt tatgcctttt
      241 cgactgaaaa ctggaagcga ccaaaagatg aagtaagttt tctcatgcaa ctacctgtcg
      301 acttctttga tacgttcgta ccagaattga ttaaagaaaa tgtcaaagtt catgtcatgg
      361 gttatgaaaa cgtattgcca gaacatacgc aagatgctgt aagacgtgca attgagcaaa
      421 caaaaaacaa tactggaatg gtattgaatt ttgctttgaa ttatggaagt agagcagaga
      481 tcgtgactgc tgtccaagaa attgccgaag aagtagcaaa aggagaaata cacgcagaag
      541 aaatcgatga tgaattgatt gctaagcatt taatgacagg tttcttgcct aaagagttac
      601 aagatcctga gctgatgatc cgtacaagtg gagaagagcg aattagtaat tttctgttat
      661 ggcagatcgc atatagtgag ctgtatttta caaaagcgct ttggcctgat tttgatggtg
      721 cgcatcttga agaagcaatc gcctcttatc aaaatcgaga ccgtcgattc ggcggtgtaa
      781 aaaaaacaga agatgagaaa ggggatcagt catgaaacaa cgtgtgatca cagcagctgt
      841 ggcgctagtc ttgtttattc cgattatatg gatcggaggc atagcggttg agcttacagc
      901 agcaatctta gcggtagtag gagtttacga gctgttccgg atgaaaggat tgacactctt
      961 aagttttgaa ggagttttat ccgccgttgg tgcagtgtta cttgttttgc caaaagaaag
     1021 atggtttttc tttcttccag agaatgtaag tacatttatg ttattttatc ttatagttat
     1081 gattctttta ggaacatcag ttatttctaa aaatacgtat acgattgatg aagcaggttt
     1141 tcctgtgctt gtcagcttgt atgtgggaat aggtttccag aattttgtca atgcacgaac
     1201 agaaagcttg gctgttttga tatttggtct atttattgtt tgggcaactg atatcggagc
     1261 atatatggtt ggtcgacgtt atggtcagca taagttaagt cctgatattt cgcccaacaa
     1321 aacgattgaa ggtgcgctcg gtggtatcgg tagtgctgtt gtagtcgcat ttctttattt
     1381 attgctatac ccagcaaaag aactattcaa tcatggtatg tttgtcatgc ttctgatgac
     1441 gattattttt tcagcagtcg gacaatttgg tgatttggtc gaatctgcaa tcaaacgtca
     1501 ttatgatgtg aaagactcgg gaaatatctt accaggccat ggtggaatct tagaccgttt
     1561 cgacagtttg ctgtttgtat ttcctatcat gcatttattt ggcattattt agtaagtata
     1621 cctgcgtttc tttgtgaatt tcataaaaga cgcaggtttt tatttttcat tatattgata
     1681 ctattggtgt ctttggtata ctatggtaaa attatagtgt tttatcgttg ttttataaaa
     1741 aacacaaaaa acaagtaaag gagaaaaaag ctatgaaaac gattctgaca tttatcatcg
     1801 tttttggtat attagtgatt gttcatgagt ttggtcattt cttctttgcg aaacgatcag
     1861 gaatcctcgt ccgcgaattt gctatcggta tgggtccgaa aatttatggg catcaagcaa
     1921 aagacgggac aacatatacg cttcgcctat tacctattgg cggttatgtt cgaatggcag
     1981 gcaatgggga tgatgaaaca gaaatggcac caggaatgcc tctttctctt ttactaaatt
     2041 ctgacggcat cgtagagaag ataaatttaa gtaagaaaat ccaattgaca aatgctatcc
     2101 cgatggaatt gagccgttat gatttagaag atgagctgac tatcactggt tatgtgaatg
     2161 gagacgaaac agaagtagta acctaccctg ttgatcacga tgcgacgatt attgaaaacg
     2221 acggaacaga aatcagaatt gcgccaaaag acgtacagtt ccaatcggct aaattgtggc
     2281 agcgtatgct gaccaacttt gctggaccca tgaataactt tattttggca atcgtgttat
     2341 tcatcattct ggcatttatg caaggtggtg ttcaagtgac gaacactaat cgcgtaggag
     2401 agatcatgcc taatggagct gcggcagaag caggcttaaa agaaaatgat gaggtagtca
     2461 gtgtggacgg aaaagaaatc cattcatgga atgatctgac aacagtcatt acgaaaaatc
     2521 caggtaaaac attagacttt aagatcgaac gagaaggtca ggtgcaatct gtcgacgtga
     2581 caccgaaatc tgtagagtca aatggcgaaa aagttggcca acttggtata aaagccccaa
     2641 tgaatacagg ttttatggat aagatcattg gagggacgcg tcaagctttc agcggttcat
     2701 tggaaatttt caaagcctta ggatcactat ttacaggttt cagtttagat aaattaggcg
     2761 gacctgtcat gatgtaccaa ttgtcttcag aagcagcaaa tcaaggtgta acaactgtta
     2821 ttagtttgat ggcgcttctt tcaatgaatc tcggaatcgt caatctgctt ccgattcctg
     2881 ccttagatgg cgggaaatta gtcttgaata tttttgaagg gattcgcggg aaaccattaa
     2941 gtcaagaaaa agaaggaata ttgacattag ctggctttgg atttttaatg ttgttgatgg
     3001 tgctagttac atggaacgat attcaacgat tctttttcta gaaaaagttg acaaataggg
     3061 tataataaca aacgataact attttgagga aataaaggag tatatagaat gagacaatca
     3121 aaaatgttga ttccaacatt acgcgaagtt ccaaatgatg cagaagtatt aagccatcaa
     3181 atcttgctaa gagctggata tatccgccaa gtatcagcgg gtatttattc atatctgcca
     3241 ttagcaaatc gtgtattgga aaaactgaaa cgcattatgc gggaagaatt tgaaaaaatc
     3301 ggagcggtag aaatgctgat gcctgccatc ttgccagcag aactttggga agaatccggg
     3361 cgttacgaaa catatggacc aaacttgtat cgtttcaaag accgcaatga tcgaaaaatg
     3421 atccttggac caactcacga agaaactttt actgatttga tgcgtaatga aatcaattca
     3481 tataagaaat tacctttaaa tctttatcaa atccaaccta aatatcgtga cgaaaaacga
     3541 cctcgcttcg gtttgcttcg cggaagagag tttatcatga aagatgcata ttctttccat
     3601 gcaacggaag aaagtttgga cgagacatat aaagattacg aaaaagccta taccgaagta
     3661 ttcaagcgtt gtggcttaga atttcgctcg atcatcggag acggcggcgc aatgggcggg
     3721 aaagactcaa aggaattcat ggcgatctca gaaatcgggg aggatacgat ctgttattcg
     3781 actgaaagtg attatgctgc aaacttggag atggctacaa gctactatgt atcaaaaaaa
     3841 tcgcatgaaa cacagcttga attggagaaa gtagctacac ctaacgtaaa atcaattgac
     3901 gaagtagcag atttcttttc tgttgaacct caaaaaatca tcaagtctgt attgttcatt
     3961 gcggatgaac aacctgtcct tgttttggta cgtggagacc atgaagtcaa tgacgtaaaa
     4021 ctgaaaaact ttttatctgc tgacttttta gaagaagcaa cagaagaaga agcacaaaaa
     4081 tatctaggtg ctgagtttgg ttctgtcgga ccagtcggtg tttcagatga cgtaaaagta
     4141 tatgcagacc gttatgtcca agatttagcg aatgccatta ctggagcgaa cgaaacagga
     4201 ttccactata aaaatgtgaa tccagaacgc gatttcactg tgctttctta tgaagatctt
     4261 cgttttgtac aagaaggaga tccttcacca gacaacaacg gtgttcttgc ctttactcga
     4321 ggaatcgaaa tcggacatat attcaaaatc ggcactcgtt acagtgaatc tatgggagca
     4381 actgtactag acgaaaatgg acgagaaaaa tttgtgatta tgggctgtta tggtatcggt
     4441 gtaagtcgtt tattatcagc aatcgtagaa caacattcag atgaacaagg aatcaattgg
     4501 ccaaaaggaa ttgcgccatt tgatctgcat atcgtccaaa tgaatctaaa agacgaatac
     4561 caaacaagcc tgacgaatga actagaagaa gcgatgacaa aagctggcta tgaagtactg
     4621 gttgatgaca gaaatgaacg cgcaggagtg aaattcgcgg attctgattt gattggctgt
     4681 ccaatcagaa tcactgtcgg taaaaaagca gtggatggaa tcgtagaagt gaaaatcaag
     4741 aaaactggcg aaatggttga agtacgtaaa gaagaattgg cgaatacttt accaatcctc
     4801 ttgaaccaag gcgaataata agcgttaaaa cacagaaaat aaaaatagct gaatcagctg
     4861 tttttgacaa tctgatgatt cgcttttaaa attggaactg cgatttgaat cgcagttcca
     4921 attttttaat gtaaaactca gaaaaaacga gtatactaaa aggtactgaa aaagaagagg
     4981 ataagatgcg atcaattgca ttgtttctca tcagaaaggg cgtatatctt tgtcagaaaa
     5041 acgagattta tttgaaaaac tgttggaaca aatccaacta gaggaagcag aaaaaaatca
     5101 tccactgctt actgctggtg aaatggatag tgtagtggtc catcgtaaat cgcgtttatg
     5161 ggaatttacg ctacattttt ccaaaatttt gcctattatg ctatatcggt cattgactca
     5221 gcatctgact atcgctttta aagatattgc acaagtcaaa ctgaatatct tagcagatga
     5281 ccagacattc gatgaacagc tcttgcaaga ttactgggcc caagctttgg aaaatcagca
     5341 atgcgataca ccactagcac agcaagtatt gaaaacgcag gtacctgtga taaaagatcg
     5401 taaagtcatc ttgccaatcg acagtacagg agctatttct tatttgaagc aacaatactt
     5461 gccattagta gaagagttgt tcgtcagtta cggattccca aaatttcgta tcgaaccaga
     5521 agtggatgag cagcaagcgg aacgtgtatt gaaacttttt gaagaacgca aacaagaaca
     5581 ggcagaagcc tttatgaagc aagcagctga aagtctagtt gtccacgaac aaaagaaaaa
     5641 ggaaagaaaa gagcaatcgc cagctcttga aggacccatc cagctaggac gaaatattcc
     5701 agccgatgaa ccaacgacac caatgatcag tatcgttgaa gaagaacgaa gagtcacact
     5761 tgaggggtac gtattcgaca aagaagtcag agaactacgt tcaaaacgca agatcttgac
     5821 tttgaaaatc acagattata cttcatcatt tatcgtcaaa aaattctcga acggcgaaaa
     5881 agatgaacaa gtatttgatg cgatcagtgt cggtagctgg ttaaaggtac gaggaagtat
     5941 tcaagaagat acgtttgtcc gtgatttggt catgaatgcg caagatatca tagaagtcaa
     6001 acatacacct cgcaaagatt atgctcctga aggggaaaaa cgagtagagc ttcatgttca
     6061 tagcaatatg agtacgatgg atgcaacaaa cagcatctct gatcttgtgg cacaagcagg
     6121 aaaatgggga caccgagcta tcgccattac agaccatggt ggtgcgcaag cctttccgga
     6181 agcacatagt gctggaaaaa aagcaggtgt gaaaatctta tacggcgtgg aagcgaatgt
     6241 cgtagatgat ggtgtcccaa ttgcttataa tgatgcacat gaagcattga gtgaagccac
     6301 atacgtcgta tttgacgtgg aaacgacagg attgtctgca gtatatgaca cgatcatcga
     6361 gctagccgca gtcaaaatgt ataaaggaaa tgtgatcgaa agctttgatg aatttatcga
     6421 tccaggacat ccattgtcac gaacaacggt agatctgact ggtatcacgg atgaaatggt
     6481 tcgagggtca aagtcggaag aagaagtatt gcggatgttc cttgaatttt caaaagatac
     6541 aattttggta gcccacaacg cggcttttga tatggggttc ttgaatacta gctacgcaag
     6601 gtatggtatt cctgaagcgg ccaatccagt cattgatacg ttggaattag ctagatatct
     6661 gtatcctcaa ttcaaacgtt tcggattagg tgtgttatct aaaaagtttg gtgttagctt
     6721 ggaacagcat caccgagcaa tctatgatgc tgaagcaact ggtcacttag catggatctt
     6781 tgtgaaagag gcaatggaca atcataacat gctttatcat gatcagctaa atgagcatat
     6841 cggagaagga gattcttaca aaagggctcg acctttccat gtaacgattt tagcaaaaaa
     6901 tcaggcagga ctgaaagatt tgttcaaact gatttcaatg tcgaatgtcg aatacttcga
     6961 acgcgtccca cgaataccac gctcccaatt gaaaaagatg cgtgaaaatc tattgatcgg
     7021 ctcagcttgt gataagggag aaatattcga agccatgatg caaaaagggg tagaagaggc
     7081 aagaaaccga gcaaaattct atgattatat tgaagttatg ccaaaagcgg tctatgcgcc
     7141 tttgatcgag caagaattgg taaaaaatga acatgatttg gaagaaatta tccaaaattt
     7201 ggtagaaatc ggaaaaagtt tggataaaat cgttgttgca acaggtaatg tacattatct
     7261 gaacgaagaa gatgccattt accgaaaaat tttgatcaat tctatgggcg gagcgaatcc
     7321 gttgaatcgt catagtttgc cggatgtcca tttccgaaca accgatgaga tgctaactgc
     7381 atttcacttt ttaggggaag aaacagcgaa agagatcgta gtagaaaata cgaataaaat
     7441 cgcagatatc tgcgaagaag tgattccagt caaagatgaa ctttatacac cgaagattcc
     7501 aggttcagaa gatgaaatat cagaattgag ttacacaaaa gcaaaacaaa tgtacggaga
     7561 tccgttacct gagatcattc aaaaacgatt agaaaaagag ttgaattcta ttaatggtaa
     7621 tggcttctcg gttatttatc tgatcgccca aaaactagtg cataaaagta atgaagatgg
     7681 ctatctagta ggttcacgtg gctctgttgg ctctagcttt gttgcgacga tgacaggaat
     7741 cactgaagtt aatccattag cccctcatta ttattgtcca gagtgccaat attccgagtt
     7801 ttttgaagat ggtacgtatg gttctggatt tgacatgcca gaaaaacaat gtccaaaatg
     7861 tggtgcacgc ttaaacaaag acggtcatga tattccattt gagacatttt taggattcca
     7921 tggagacaaa gtgcccgata ttgatttgaa cttctcaggt gattatcaag cagaagccca
     7981 taactatacg aaagtattgt ttggcgaaga ctatgtttac cgtgccggaa cgattggtac
     8041 agtcgccgac aaaacagcat atggctatgt aaaaggatac gaaagggaca ataatctgca
     8101 gtttcgaagt gcagaagtcg acagattagc aaaaggcgct actggagtca aacggacaac
     8161 cggccagcat ccagggggga tcatcgttat tcccgattat atggatgtct atgattttac
     8221 gccgatccaa tatccagcag acgatcaaaa ttcagaatgg aaaacgactc attttgattt
     8281 ccactcgatt cacgataatg tattgaaatt agatattcta gggcatgatg acccaactgt
     8341 tattcggatg cttcaagatt tatcagggat cgacccacaa acgatcccta cggatgaccc
     8401 ggaagtgatg cggatatttg ccggtcctga agtacttgga gtaagtcagg aacaaattta
     8461 ttccaaaaca gggacattag gtattccaga attcggcact cgttttgttc gagggatgtt
     8521 agaagaaacg catcctacaa cttttgcaga actactgcaa atttccggac tatctcatgg
     8581 tacagacgta tggttaggaa atgcggaaga attgattcga cgaggagatg cgacactggc
     8641 ggaagtaatc ggctgtcgtg atgatattat ggtttatttg atccatgctg gattagatag
     8701 cgggatggca tttaaaatca tggaaactgt tcgtaaagga caatggaaca agatccctga
     8761 tgaattgcga gaaacctatc tatcggcgat gaaagaaaat aatgtgcccg actggtatat
     8821 cgattcttgt tcaaaaatca aatatatgtt tccgaaagcc catgctgcag cttacgtatt
     8881 gatggctctt cgtgtcgctt atttcaaggt ttatttccct attttgtatt actgtgcata
     8941 cttttctgta cgtgcagacg actttgatct agtctccatg tgcaaaggaa aagatgctgt
     9001 gaaacaagca atgaaagaaa tcacggacaa gggattagat gcttccgtaa aagaaaagaa
     9061 tcaactgaca gtgctggagt tagccaatga aatgctggaa cgaggattca aatttggaat
     9121 gattgatttg tataaatcgg atgccgttaa ctttgttatt gaaggtgata cgttgatcgc
     9181 accatttaga gctgtcccaa gtttgggaac caacgtggcg aaacaaatcg ttgaagcaag
     9241 aaaagacggt ccatttttat ccaaagaaga tcttgccacc cgcggtaaag tttcaaaaac
     9301 attgatcgaa tatatgaacg acaatggcgt attgaaagat ctaccagatg aaaatcaatt
     9361 atcgttattc gatatgctat aagcatctgt tttcctgtct gcttgtaatt ttaagcagag
     9421 tatggtatag ttcttatagg taatgatact aactgtgagt gagcggattt ttcgctcact
     9481 ctttttaatg gaattacgca cttaattttt aaggaggcga aacttttgag tagcgtcgtt
     9541 gaaacagtca cagaaatggt gacaccgatt ttagatgaac aaaattttga acttgtcgaa
     9601 gtcgaatttg taaaagaagg aaaaagctgg ttcttacgag tatttattga caaagaaggc
     9661 ggtatcgata tcgaagaatg cgcctttgtc agtgagaaac tgagtgaaaa acttgatgca
     9721 gcagatccag atccgattcc tcaagcttat ttcctggagg tatcctcccc gggagcagag
     9781 cggccattga aaaaagaaag cgactatgaa aaggcacttg gtgattatgt ccatgtctcc
     9841 ttataccaac cagtagatgg cgaaaaacaa tatgaaggat ttcttcaatc atttgatgct
     9901 gaacagctta cattgaaaat acgcataaaa acacgagaaa aagaattgac ttttgaccga
     9961 aaaaatattg caaaagctcg cttagctatc caattttaat cacggagagg aatcagaaaa
    10021 aaatgagtaa agaaatgtta aatgcgttag acgctttaga agctgaaaaa gggatctcaa
    10081 aagaaatcgt gatcgatgct ttagaagcag cattggtttc tgcatacaaa cgtcactatg
    10141 gccaagcaca aaacgtcgaa gtggaatttg atcagaaaaa aggaaagatc catgtctatg
    10201 cggtaaaaga agtaacggaa gaagtgatgg attcacagtt ggaagtctca ttgaaagatg
    10261 cacttttaat caatccagca tatgagatcg gtgatacgat ccgttttgaa gtaacaccaa
    10321 aagattttgg acggatcgct gcgcaaacgg ctaaacaagt tattttacag cgtgtacgtg
    10381 aagcagaaag aacgattatt tataacgaat tcagtgctta tgaaaaagac atcatgcaag
    10441 gaatcgtcga acgccaggat aaacgttata tttatgtcaa tcttggtaaa attgaggcag
    10501 ttctttctaa acaagatcaa atgccaaatg aattttatca accacatgat cgaatcaaag
    10561 tttatgtttc ccgagtagaa aatacgtcta aaggtccgca agtatttgtt agccgcagcc
    10621 atccagacct attgcgtcgc ttatttgaac aagaagtacc agaagtctat gatggaatcg
    10681 ttgaaattgt cagcatcgcc cgtgaagctg gcgatcgttc aaaagtggca gttcgctcaa
    10741 atgatccaaa tatcgatgca gtcggtacat gtgtcgggcc aaagggacaa cgagttcaag
    10801 caatcgtcaa tgaattaaaa ggcgaaaaca tggatatcgt cgaatggaat gaagatccag
    10861 ctgtattcat cgcaaatgca ttgaatcctg cacaagtcat ggatgtgatc ttcgatgaga
    10921 caaatccaaa ggcctgcaca gttgtcgttc cagactacca attatcgcta gcaatcggta
    10981 aacgcggaca aaatgcacgt ttagctgcta aattgactaa ccataaaatc gatatcaaat
    11041 cagaatctga catgacggaa ttttatgaaa atcaagaaca acaaaaagaa acagaagaac
    11101 tgcatgatga agcaatcgtt cagtcagatt taacagacga tgaatatgaa acaattgcat
    11161 tcaacaatga aacggtcgaa gaaaaaccag aagcttaact ttagtaagga ggcaaagaga
    11221 tgaaacaaag gaaaattcct ttacgcaaat ccgtggtttc aggtgaaatg aaacctaaaa
    11281 aagagatggt ccgaatcacc cgttcaaaag aaggggaagt atcgatcgat ccaactggaa
    11341 aaatgcctgg acgaggagcg tatgtctcac ttgagccgga agaagtacaa caagcttggg
    11401 ataaaaaaat tctcgatcga gtactcgaaa caacattaac agatgagttt tatcaagaac
    11461 ttcttgacta tgtcactcac caaaaagcac gtaaagagtt atttggaaaa tgaatacagt
    11521 taatcggcaa aaagccatga atctcatcgg tctagcgatg cgcgcgggaa aactaatcac
    11581 gggtgaagaa ctgaccattg ctgaaatccg caaacaaaaa gcaaaattgg tgtttgtggc
    11641 aaccgatgca agtgagaata caagaaaaaa gataaaagat aagagttcat attatgaagt
    11701 tccttgcttt gagctatttt ctgaagcaga aatcacgcag atgatcggaa aacctcgcaa
    11761 ggtattcgga ataatggatg ccggatttgc gaagaagacc aaggagctaa ttgaaggtta
    11821 ggaaggtgat tgcatgggca acaaaagaat ctatgaatta gcaaaagaac tcaaaaaatc
    11881 tagtaaagac gtcgtggaaa aagcacaaca acttggcatc gatgtgaaaa accatatggg
    11941 tgcgattact agcagtgatg agaagaaatt gcaacaagcg ttcaaaaacc caagcacgaa
    12001 acaacaagct gcacaacaag caagcagaaa accagccaat caacagccga acgatcaacg
    12061 taaaaaagaa acaacgaaca atcaacaaaa aaataaaaat aaccgcagtt atcaagatcg
    12121 cggtcaaggg agcggtcaag tgaatcaagg aaaaaaacca acaacaaacc aaaataatac
    12181 gggaaatacc agcagccaaa atcgtcaagg aacgagccaa ggcggcaatc aaaaccgtac
    12241 taacaataac aacaaaccaa acaataacaa tcgcggcaaa tttaataata ataaccgtaa
    12301 tcgttataat aaaaaaggta aaaaaggcaa acagcaacaa tcaactaaac cagctgtacc
    12361 accacgcaaa ttccgtgaat tgccagatgt attagagtat actgaaggta tgaacgtagc
    12421 agatattgcg aaaaaaattc atcgtgagcc agcagaaatc attaaaaaat tatttatgct
    12481 tggtgtcatg gtcaatcaaa accaagcact ggataaagat acgattgaac tgttagcgac
    12541 ggactatggc atggaaccac aagaaaaagt ccaagttgac attgctgata tcgataaatt
    12601 cttcgaggca gaagaagtcg atccagataa attagtctca cgtccgccag tagtaacgat
    12661 tatgggacac gtagaccacg ggaaaacaac cttattggat acattgcgcc attcacgtgt
    12721 aacaagcggc gaagccggcg gaatcacaca gcatatcggt gcctaccaga tcgatattga
    12781 tggtaaacca atcactttct tggatacacc aggacatgcg gcctttacaa gtatgcgtgc
    12841 acgtggagca agtatcacag atatcacgat cttagttgtt gctgcagatg atggcgtcat
    12901 gccacaaaca gttgaagcaa tcaaccatgc caaagctgca ggcgttccaa tcatcgtcgc
    12961 tgtaaataaa attgataaac caggtgcaaa tcctcagcat gttatgcaag aattaagtga
    13021 gtatgaatta attcctgaag catggggtgg agaaacgatt tttgttgaga tctcagcgaa
    13081 attcggccaa aacatcgaag aattgctgga aatgatcctg ttagtcgcag aagtagaaga
    13141 tttaaaagca gatccaacac aacgcgcaat cggaacagtg atcgaagcac gtcttgataa
    13201 aggtaaaggt cctgtgtcaa ccttacttgt ccaagaagga acacttcacg ttggtgaccc
    13261 aatcgttgtc ggtaatactt acggacgtgt ccgtgtaatg acaaacgatc ttggccgtcg
    13321 cgataaagaa gcaggacctg caacgccagt agaaatcact ggcttgaatg atgtacctca
    13381 agcaggtgac cgtttcgtag tcttcgaaga cgaaaagaca gcacgtgcag cgggtgaaga
    13441 acgcgcaaaa cgtgcattat tggaaaaccg tgctgccaca agccgtgtga ctttggacaa
    13501 cttatttgaa agcctgaaag aaggcgaatt gaaagaagtt aacgtaatca tcaaagcaga
    13561 tgtacaaggt tcagcagaag cactggctgc atcattgaag aagattgatg tggaaggtgt
    13621 ccgtgtaaaa atcgtccatt cagctgtcgg agcaatcaat gaaagtgatg taaccttagc
    13681 agcagccagc aacgctatta tcatcggttt caacgttcgt cctacaccgc aggcaaaagt
    13741 gcaagctgat actgaagaag tcgatattcg tcttcaccgt atcatctata aagccatcga
    13801 agaaatcgaa actgcgatga aagggatgct agaccctgaa tttgaagaaa aaatcaccgg
    13861 tcaaatgatc gttcgtgaaa ccttcaaagt ttctaaagtt ggtacgattg caggtgccta
    13921 tgtaacagaa ggatatattc gtagagatag cggtgtccgt gtaatccgtg acggaatcgt
    13981 aatctatgaa ggccaacttg caagtctgaa acgattcaaa gatgacgtga aagaagtcaa
    14041 aatgggttat gaatgtggtg cgatgattga aaaattcaat gacatcaaag tagatgacgt
    14101 aattgaagga tttatcatgg aagaagtcaa aaatgactaa aaactgataa aaggaggcca
    14161 atcattatgg ctaactaccg tgatcgccga gtcggtcaag aaatcttaaa agaagtcaat
    14221 gatattttgc gcaaaaaggt ccgtgaccct cgtgtggaga acgtaacgat cacagatgtg
    14281 aaagttacgg gggacttaca gcaagcaacg atctattaca gcattttatc tgacttagca
    14341 tcagataaga aaaaagcaca agatggattg aataaagcat caggattgat tcgtcgagag
    14401 ttagggcaca atctaagtat ttataagaca cctgaattgt tctttgaact tgatgaatca
    14461 gttgtctatg ggaaccatat cgatgaattg cttcgcgact tgaacaaaga ctaatcaaaa
    14521 aatcccgcaa atcctgtgaa atcaggggtt gcgggatttt tttgttttca ttaagacgta
    14581 ttacctaaga gttccatgac tttagtctgg tagcgtgtca gctaaccaaa aaagagaaaa
    14641 agaaaagtaa aaaattcgac tttctttttt ctatagtata taaatttttg catatagata
    14701 aagatcaaga taccgataac ggaagctaga ttgtaatcta ttttagtgac agtcaggtat
    14761 agccaagtaa ctagaagatc agtggaacct gcaccataaa agtttgagtt tgtcggtccg
    14821 ttgacagtta aaagaaaaat cacgttgaag ttattgatat ttccgattac gccgcttcga
    14881 tttggtcacg atccaaattt tggataatcc ctgtagaaat tagcatagta gctgggatac
    14941 caattcacat attgacaaag ataacagaga cttttacata aaacggatca atcaggaaag
    15001 gaattgctcg atccagcccc agttttccaa caatgtgtta actggcctag cgtcattcag
    15061 caaattatgc atgatgagga gcgaaatgaa ctgcggaaca gccattgtga taacgacaag
    15121 ttttttggat caatgtaaaa aacgtcagcg aaaggaaaaa tttcgtggtt ttttatacct
    15181 gttcatttgg ctagatataa aatccaaaca aatgaacaga agtgcttatt aggtttcaat
    15241 tgggagtatc tgcgctagaa taagagtaat aatgaaaagg agtgttatgt atggattatt
    15301 ctagtaaaga atactttgat aaaatgacgg cttggtggcg tgctgctaat tatctttctg
    15361 ttgggcaact ttatcttaaa gataacccct tactgagacg tacactgaaa ccagaagatg
    15421 tcaaaaaaca tccaatcggt cattggggaa cgattcctgg ccaaaatttt atttatgtcc
    15481 atttgaaccg agtcatcaat aaatatgatt tgaacatgtt ttatatcgaa gggccaggac
    15541 acggcggaca agtcatggta tcgaatgctt atttagatgg aagttacaca gagatctatc
    15601 cagaagtaac ggaagatgag acaggaatgc aaaaattatt caagcgattt tcatttcctg
    15661 gaggcattgc ttctcatgca gcaccagaaa caccaggttc tatccatgaa ggcggagagc
    15721 ttggatactc tctttcacat gcagtcggag ctgtattaga caatcctgaa gtaatctctg
    15781 ctgtcgtgat tggtgatggt gaagcagaaa ccggtccttt agctggaagc tggttctcaa
    15841 atgtctttat caatccagtg atagatggag ctgttttacc gatccttcat ttaaatggtg
    15901 caaaaatagc caacccaacg atcttggcac gtaaatcaga tggagaatta gcaaattact
    15961 ttaacggact tggctgggag cttttcttta ttgaaggaaa tgatccagaa aaattaaatc
    16021 ctgttatggc tgaaaaaatg gatcaagcaa tcgagaaaat caaatccatc caaaaagaag
    16081 ctcgcctgaa gacagcaaca gatgtcgtga tgccaaaatg gccagtattg atcgtacgca
    16141 caccaaaagg atggaccggt ccaaaagaat gggacgggga accaattgaa ggaaccttcc
    16201 gagcacacca agtaccgatt cctgtagatc aagaacatat ggatcatgca gatgctttac
    16261 tccgttggct gaaatcctat gaaccagaaa aattatttga tgcacaagga cgtattttag
    16321 aagaaatcag agaaattgcg cctactggtg accaaagaat ggcaaaaaat cctatcacca
    16381 atggtggaat cgatccaaga ccgcttatta tgccagattg gaaaaaatat actttgcaat
    16441 ttgaaaaacc aggctccgtt aaagcagaag atatgaccga acttggtaaa ttcgttcgcg
    16501 agatcattga gaaaaatcca gaaaatttcc gtatatttgg accagatgaa acaaaatcaa
    16561 accgattgaa tcaagttttc aaaacgacaa atcgccaatg gatggaaaaa atcgaaccag
    16621 aaaatgatga atggctttcg ccatctggtc gcgtcatcga cagtcaatta tcggaacacc
    16681 aagatgaagg gttcttagaa gggtacgttc tgactggacg gcatggtttt tttgcaagtt
    16741 acgaatcatt cttgcgagtg gttgactcta tgttgactca gcacttcaaa tggatacgga
    16801 aatcacatga cctttcttgg cgaaaagatt atccttcgct gaatctcatt gcaagctcaa
    16861 cagtcttcca gcaagatcac aatggatata gccaccaaga tccaggaata ttgacacatt
    16921 tggcagaaaa gaaggcggag ttcatccgtg aatatctgcc tgcggatgca aatacattgc
    16981 tcgcagtcat ggataaagca ttccgctcgt cagaaaaaat caatttgatc atctcttcca
    17041 aacatccgcg tgctcaattt tactcagcag aagaagcagc cgtcttagtg aatgaaggct
    17101 tgaaaatcat tgattgggct tcgacagcaa aagaggagga acctgaattg gtcattgctg
    17161 ctgcaggaac ggaatctaac ttagaagcat tagcagctgt aactttgctg ctggaagaat
    17221 tcccaaaact aaaaattcgg ttcatcaatg tcgtagattt attgaaatta cgtcatccaa
    17281 gtcaagatcc gcgtgggttg agcgatgaag aatttgatca atattttaca aaagacaaac
    17341 cgatcttatt tgctttccac ggttatgaaa cattggtccg cacgattttc tttgatcgtc
    17401 ataaccatca tttaatgatt catggctaca aagaaaatgg agatatcacg acaccatttg
    17461 acatgcgtgt agtaaatgaa cttgatcgtt atcatttggc aaaagacgca gctttaaaaa
    17521 tcaaaggaag tcaagcagaa gattttgcgg aaaaaatgga tcagaaatta caagaacatc
    17581 aaaactatat tcgtgaaaat ggtattgatc tgccagaagt attggattgg aaatggaaaa
    17641 atcttgacca ataaaatgat aaaatcgatc ccgcaaaaca gaatttttta cattgtatta
    17701 tttgtcaaat taag
//
LOCUS       DACECY010000056        17568 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.060, whole genome shotgun sequence.
ACCESSION   DACECY010000056 DACECY010000000
VERSION     DACECY010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.060"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            282..521
                     /locus_tag="IWO38_002048"
     CDS             282..521
                     /locus_tag="IWO38_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314129.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(883..1143)
                     /locus_tag="IWO38_002049"
     CDS             complement(883..1143)
                     /locus_tag="IWO38_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314130.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1328..1825)
                     /locus_tag="IWO38_002050"
     CDS             complement(1328..1825)
                     /locus_tag="IWO38_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ0314131.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1955..2665)
                     /gene="budA"
                     /locus_tag="IWO38_002051"
     CDS             complement(1955..2665)
                     /gene="budA"
                     /locus_tag="IWO38_002051"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ0314132.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="IWO38_002052"
     CDS             complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="IWO38_002052"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ0314133.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4547..5209)
                     /locus_tag="IWO38_002053"
     CDS             complement(4547..5209)
                     /locus_tag="IWO38_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0314134.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5219..6034)
                     /locus_tag="IWO38_002054"
     CDS             complement(5219..6034)
                     /locus_tag="IWO38_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0314135.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6296..6739)
                     /locus_tag="IWO38_002055"
     CDS             complement(6296..6739)
                     /locus_tag="IWO38_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ0314136.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6873..7211)
                     /locus_tag="IWO38_002056"
     CDS             complement(6873..7211)
                     /locus_tag="IWO38_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0314137.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7199..7576)
                     /locus_tag="IWO38_002057"
     CDS             complement(7199..7576)
                     /locus_tag="IWO38_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0314138.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7800..8993
                     /locus_tag="IWO38_002058"
     CDS             7800..8993
                     /locus_tag="IWO38_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0314139.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9159..9581)
                     /locus_tag="IWO38_002059"
     CDS             complement(9159..9581)
                     /locus_tag="IWO38_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0314140.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            10171..10626
                     /locus_tag="IWO38_002060"
     CDS             10171..10626
                     /locus_tag="IWO38_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314141.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            complement(10788..11960)
                     /locus_tag="IWO38_002061"
     CDS             complement(10788..11960)
                     /locus_tag="IWO38_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0314142.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(12166..13595)
                     /locus_tag="IWO38_002062"
                     /pseudo
     CDS             complement(12166..13595)
                     /locus_tag="IWO38_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            complement(13592..15094)
                     /locus_tag="IWO38_002063"
                     /pseudo
     CDS             complement(13592..15094)
                     /locus_tag="IWO38_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345213.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            complement(15091..17418)
                     /locus_tag="IWO38_002064"
     CDS             complement(15091..17418)
                     /locus_tag="IWO38_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0314143.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         5067 a         3480 c         3050 g         5971 t
ORIGIN      
        1 tatcctccta gcaactattt aaatacccaa aaaaattacc tctagataat atcataccta
       61 cgttttcact tataaatttt ttcataaaaa aagcgtttag aggcataatc gttaatacag
      121 atggtaacca atgtatctgg tttgaactgg gatatcaccc tctccatata tgtattacta
      181 agactaatag atactataaa agacttgata gttttgcatt gattttgcct aatactgagc
      241 ataggtgtat gctaaacgat actataagga gtgtgtacat aatgaaaaga attctgttta
      301 tcagtttatc aaatttattg ttacttactg cttgtgggac ttctgcatca aagaacatac
      361 cttcagatac gcaaatttcg aatagtatcg attcaacaat ttctacaaat gagaaaaatt
      421 ctccggtaga aacttccgat ataaaagttt ctacctctgc aaaaaacagg gtaaagaaac
      481 tgtcagcacg tctcctttat ttcaaacaaa ctctgaatta gcttcttcat cttcttcagc
      541 aaataatgat gatagtttcg cgtttactcc taactaaatg caacctttac aaatgcttta
      601 cgataacaag cctgagctaa atgatcctga tatcagtttt gcattttttg aaatgattgg
      661 atcggatgat ctttttaaaa ctactagttt gtctatccga aaataggaca gttctggcag
      721 tataggtttt tatcgagttt ctccaccagg cctcgttaca gagacagatt ctctaggcga
      781 accttttgaa taaatcaaaa atcatgaaca atcactgcca actaaataaa gtgccttcaa
      841 aagcttggtc ttctcgcttt tgaaggcact ttatttaaat ctttatgaaa ctaaagtatg
      901 ttcatatttg ttgctatagt tatatagata ttctgttgtt tccaactcat aaacgtgagt
      961 atattttgcc actacttttc cgcatttttc taatatttca ttgcgatcga cgtcatgata
     1021 ttcgtctacg cagatcacaa cacaattttc taatttactg attacttctc caaccacttt
     1081 ttgtttcaag ccaataggtt ggcaaatata tttttttcct aaaatgatag tttcgttatt
     1141 catatttcat ccatccttaa tgtttttcca ttatttaaaa tgataatacc attaaaatat
     1201 gattatgaaa atcaataaca cttgtgattt tataaaaaaa taaaaaaacc gaaaaacaga
     1261 aatgcccact gtctttccgg ctctttttat tcttgatttc tcttcttttt tgtttcaata
     1321 gacgatttta gttattaata aatgaaaaag catttatatt aaaatttgct aataaaagat
     1381 ctacatattt ttctagaaat gcttgatcga tttcatcata ccctttcgtt actccgcaat
     1441 cgatatctaa aacaccgatt aaattacctt cttttaccat tggcactacg atttctgatt
     1501 gtgcggctga atcgcaggaa atgtaatttg catgggtttt gacatcatca acgatcaatg
     1561 tttcttgttt ttcagctgat tctccacaaa ctccttttcc catcttgata cgtgtgcaag
     1621 aaactcgccc ttggaacggt cctaaaatca attcattacc atcgaataaa taatatcctg
     1681 caaagacagt ctcaggcaaa gtctcattca acaatgcaga tgaatttgct aaatttgcta
     1741 accagttatc ttctgcttct aataatcctt gttgctgtaa taataaaatt tcgtatgcat
     1801 cttttttctg tttttctgtc cacatgataa aaaccgctcc tttttgatct atggagaaga
     1861 ttatagctca gtttttttgt aaacgctagg tttcctcctc aaaaaaacga gatacgtcag
     1921 gttaatcacc ttgaacgcat ctcgctgtct ttttctattc tgcttgttgg atttctgctg
     1981 ctaaatcagc ataatcaatc gtttcgtgaa caaaagtcgg atcatcaact ggaaaatgct
     2041 gtacaaggga cgagatgcgg ctaatctcaa cttttccttc agccacttca aaatccatta
     2101 catgtccgcc aaatgtatgt gtatcatcga taaaatgcaa gtgaaatccc gctgcagcaa
     2161 ctccttggaa taattcaggc gtaaagaacc cgactaatgt tccttggaca ttgctttgtt
     2221 ggaattctgg ttgagactct gaaatttttg ctagtcttct gtaaggtttt tcttgcttcg
     2281 gcatgatacg gatgtgcata tttttgaata atccagttat cttcactgcc aaaaataggt
     2341 tttccccggc ttctcttact aatatgtctt ttttcaattg ttctccatct gcagtttttg
     2401 agacagaaaa actagtatct ggcgtaaaat ctgtaattgc tgcatagggg gtaagttcac
     2461 ttccatctaa ttgggcaact gagccatctg atcttccttg ataagctacg ccatctaaaa
     2521 agatgacttc accatcaagc ccatgcaatg ttcccaatcc cagagttcct ttttctaata
     2581 tatgagcaat cgtttctgtt ccatccatca agccagccat caaagctcct aatgtcccgt
     2641 gctggtataa gattttttca gtcatgtcga cacttcctta atagaattga tctggtaaga
     2701 ttgtttctcc caatttctca ttatcacggt aatcaatcgg aatatcaata attacgggtc
     2761 cttctgtcgc aaatccttct tctaaagctt tttctaattc ctctacactt gtagctcgga
     2821 ttcctttagc gccaaaagct tctgcatatt tcacaaaatc aactggacca aagtctacac
     2881 ctgacgcccg ctgatatttc attttttctt ggaactctac catattgtag tgtccatcat
     2941 tccaaatcaa atgaacgata ttcaattttt tacgtaccgc cgtttctaaa tcctgggcag
     3001 agaataaaaa tcccccatcg ccggaaactg atacgatttg tgtatttgga cgcaccaatg
     3061 cagcagaaat cgcccatggc aatgcaacac caagcgtttg cataccatta ctgaataaca
     3121 aatgacgagg ttcataactt ctaaagtggc gtgccatcca aatataatga ctacctacat
     3181 ctacggtaac agtcatatca tctgttactt ttgactgaag cgtgttaata acttctaaag
     3241 gatgcaagat acctgcttct ccttttgatt gaacaatgtc ccgctccgtt aattctgctt
     3301 gaagagatgc taaatattct tttgcatctt ctgacacttg ttgaggctct aatgaacctg
     3361 tcaataaatc aagagtggct gagatatcac cgatcaattc tctttcaggc tgcataaaag
     3421 gatcgatttc agctggcgct tcatcgatca caatgatccg cgcatctttt tccgcattcc
     3481 agtttcttgc ttcatattcg attggatcat agccaatagc aataactaga tcacttcgtt
     3541 tcaaaagcat atctcctggt tggtttctaa ataaacccac acgaccaaag aaatgcgctt
     3601 ctaattcacg tgagatcact ccagctgctt ggaatgtctc tacaacaggc aatgctgtct
     3661 ttccaactaa ttgcctgatg gctaacgttt ctttttcact tgatcctcgc atacctacta
     3721 atagtaccgg tagttttgct tctctaatgc gttcagctag gtaacggata tcatctgcag
     3781 aagcagaacc taatttagga tcgctcaatg gcttgatgac attcccttgg acaggtgcat
     3841 ccactacatc ttgcgggata ctgataaagc tcgcgccttt ttttgaactc ttagccatac
     3901 gataagcatt ggcaatcact tctgaaattg tttctggatc ttggatttct gcactgtatt
     3961 ttgtgattgg ctggaacagt gctgcattat ccatgctttg atgggtcaac ttcaatagat
     4021 cgcttcgttt tacttgtccg gcaattgcta aaactgggtc accttctgct gtcgctgtta
     4081 ccaaacctgt agctaagtta ctagcaccag gaccacttgt tgcgataact actcctggct
     4141 ctcctgtaat acgtccgatt gcttgtgcca taaatgcagc attttgttcg tgacgagtca
     4201 cgattaattc aggaccttga tcctctaatt cattaaatac accatcgatt tttgcacctg
     4261 gtataccgaa tacataatca acatgatgat taatcaaact ttctactaca gatttcgaac
     4321 cttgtttcat tttttcgtcc attgaacatc gtccttttta tgctttattt ttaattatta
     4381 gttagaaata atcacaaata attgatacag tttctaaacc tgtttcattc aaaagtatag
     4441 cacgttttat ttcatttgtt tagccatatg tttcaaaaaa tgaaaaagaa tgacagtttt
     4501 tacataaaaa ctgtcattct taattgaaag tttcaattta taaagtctac agatccatag
     4561 ttctcatcaa tttacgattg ttgaagtaaa tcaaaccaaa tacgatagaa ggacctagta
     4621 gaatacacca ttggggcttt acgaaaaaga agagaaacaa acttgtcaat agtaaaaaat
     4681 ctagtaaact cacataccat tttttcaacg tatagtagaa cgctatccgt agaacttgat
     4741 ttggtttcga cgctggattt ctcacctgaa aataccagta gttgatgcta aaggaaactg
     4801 ataaacaagc aagtaaaaca aacaacggaa tcaaccattt tccataagaa aaacgtataa
     4861 aaaagaaaca atcaaaaatc attatcacac taactatcca agcaaatagc caataaaaaa
     4921 atccctttag ccaaaattgg cgaaacgtct ttatgaacaa acgtactggt gaaaaacttt
     4981 tttcttttgc taattcttct gtcataccta tcatcacgat gcaacctgga ccaataaaaa
     5041 gtaaagaaaa aataaaggaa aagacattcc ttccatctat cgacaaaagg aacgcagctg
     5101 ctatatacaa gatattgaca caccaaaaac aaaggctact tgccaatgat tgatacaacc
     5161 aataaaagaa ttccatataa acatttttgt caaaacgctg ctctttcata atcacctcct
     5221 attccttcat tcctgatgaa gcgatccctt ctacaaaata tttttgcatc gctaaaaata
     5281 taaaagcaac aggaagaaca gaaacaactg aggcagccat gattaatgcg tactctgcat
     5341 caaatagccc aataaacatt ttcaacccta actggattgt tttattttcc gtagaagaca
     5401 aataaatgaa tggccccata taatcgttcc atgtattaac gaatgtgatg atcgtcaaac
     5461 tggcaatcgc cggttttgta agaggtaaaa tgatcttgcg atagattccc cactctgata
     5521 agccatcgat ccgcgcactc tcacataaag aatctgggat actactgtag tattgcttca
     5581 acaagaacac accaaaagca ttgaacgctt gtaatagcac caacgaccac aacgtatatg
     5641 ttaaacccac ttttctcatc ataatgaact gcgggatcat ataagactgc caagggacag
     5701 caatcgtgcc gatatatgct aaaaataaaa tatcgcgccc tttaaaattt agtttagaaa
     5761 acccgtaagc agcaaaacta gaagtaaaca actgaatcaa cgtgatcaca acgcttaaaa
     5821 aagctgtatt cttaaaaaat gtcactaaag gaatacgaga ccaaataacc agataattct
     5881 ctaaatgcca agttttaggg atccattgaa tcggaatcgt gaacacttca ttatttgctt
     5941 tgaatgaagc agaaaccatc cataaaaaag gaatgatcgt caataaagct aatacaatca
     6001 gcagaatcat acccaaccat tgaataaagg gcatgttttt tgaacgaatt tttgcttttt
     6061 ttctttcatt tcgaacacct ccaaagttat aatcttcatt tctttgtaat gattacaatc
     6121 cagaacaacg gttattatag ctgattcatt tccgttcttc ttatgtttgt aagcgcttat
     6181 ttaacaagtt atgtaaatct ctttattttt tttgatttaa aaatgaaaaa atacaaaagc
     6241 aagactgtca tacgtctcgc ttttgtattg aaatttcttc gattagagat acttattact
     6301 gttcaaaagc ttctttgaaa cggtgaatca attcattgac tacaggtgat aaaatccgat
     6361 tctttttcca gactagcatg cagttcgttt caatcattgg gtcaagcgga acgaatgtca
     6421 ctcgctttga aagatgactt acagcacctt ctatgactaa agcagcacct acctgattct
     6481 caattagcgc aaatacattg aaaatcaaat tgtacgttcc tgtgatcagc aactgctcct
     6541 tttttatgcc taaccagtca gcaaacattc gctgtatttc tggacgcttt gagatcaaaa
     6601 gaggtacatt cgcaaagtct tcttttgtta tcacgtcttt ttttgctaaa acagaatttt
     6661 ctaaaatgag caatccccat ttttcctttc ttggtaacaa aatcgtttcg tatttctctg
     6721 tatctactgg atctagcaaa atcgctaaat ctaacaggct tttttctagt cactccgtga
     6781 tatcatcact tgttccgctg tggatatgaa attgaacgct gggataatca cggatcaact
     6841 tttctagtaa tagtgccaat gtattcgaat tatcagcttc cacacagccg atcgtaaatc
     6901 ggccgctgaa taattgtttt tttctgttct cgaattcttg agtggtttga ttcgttaaat
     6961 ctaaaatttc ttctgctcgg cttttttaga aaataacctg cttctgtcaa gatcattttg
     7021 cggttttcgc gtacaaacag ttctgttcct agttcctctt ctaactcctt cagttgtcgg
     7081 cttaaggttg gttgtgtaag atgtagaact tctgcggctt gagagattgt tccttcttct
     7141 gcaattgtcc aaaaatattt tagtaagcgt aactccatag cgatgctcct tgattttttt
     7201 acttgataca ttccatttta tttctagata acacacgatc atatttcttg atttttcctt
     7261 gtaaacgttc caaacattct tgcatttcag caattttttt cgtcaaaagt tcactttctt
     7321 caatcaaaag cttacgccgc tcaaatacag ttgcttctcc ttcgctagat aggcgaatat
     7381 attcaatcaa agactcgatc gataatccgg catttcgcat acagatcgta aagtcgaccc
     7441 attttaggtc ttcttctgta taatcacgga ttccgccttt tgtccgatta actggtggaa
     7501 tcaacccgat tcgttcataa tagcgcaatg tatcggccga aatgcccttt tcttcactga
     7561 cttgtttgat attcatctga tcactctttc tcttaatttc ttgccacttt acttatgtat
     7621 tttatttatt ctacaacttt aagtgtactc caagtcaata ctcatttcta ttaaaactaa
     7681 gctggattat ctatatcggt tgtttgtgta ttatttcaaa taaaaaagtc ttgttttttt
     7741 attctttttt ggatatactt ttaacaattt agggaaattt taggaaaagg ggtataaaaa
     7801 tgacaacgta tcagcgaaag ttattattat cgacatcggc gggaattgct ttagagaata
     7861 tggatattat gtttttagct ttttcattat cttcaatgat tacttctttt catattagtg
     7921 gtacgcaagc gggtttcatt tcaacgatca ctaatttggg gatgctcatt ggaggtattt
     7981 tttttggcat catagctgat aaatatggac gtgtgaaagt attttctcaa acggtccttc
     8041 tgttttcgat tgcttcttta ttgatgtatt ttgcttcaaa catctacttg gtttatctct
     8101 ttcgctttat cgcaggtgta ggagcgggag gagaatacgg ggcgtgtatg tccttgattt
     8161 ccgaaagttt ttcaaaaaaa caaattggac gagcttcttc ggttgctggt attggtgcgc
     8221 aggtcggtgc ggctttggct gcgattttag ctgctgtgat cattccaacg tttggttgga
     8281 agatgttgta tgtcgtaggt gtactgccgg tgttgatggt cttgttcatt cgtcgcggct
     8341 tgactgaacc aaaggaattc aaatctgtac aatctgccga aaagaaaacc aaacttagca
     8401 gactgtttga atcaaaacat cttgcttggc aaacatgtgg ccttagtatc atggtaactg
     8461 tccaaattgc cggctatttt gggttaatga attggctgcc ttcaattatg caaaatcgat
     8521 tagggctttc ggtttctggg tcttcattgt ggatggtcag tactatttta ggcatgtctt
     8581 tggggatgat gacttttggc ataatcatgg ataaattggg gcctaaacta gccttttctt
     8641 tgtttttaat ttgttcatcc ttatctgtct atctacttgt gttagcccat agccagtgga
     8701 gtttggtagc agccgctgtg atagtcggct actttatcaa tggcatgtat ggtggatatg
     8761 gcgcagtgat tagtagtttg tatccaacag aaatccgcgc gacggcgaat aatttcatta
     8821 tgaaccttgg acgagctgtt ggtggttttt cttctatcgt catcggtttc ttgatggatc
     8881 attatagttt aactgccgta atcttgtttt tgagcggtat ttacctagtc agtctgctgg
     8941 tcttgacaac tcttactggt gttacagcat taagggaaag cttgcagcat tgatagttga
     9001 gtctgtaaat gacaagaaag aggtgtggca aaagcttgca tctgcggtaa taagaccagc
     9061 caaatcaaaa acatgagaca ctgtttccgt ttggctggtc gtattacttg atgcagggca
     9121 gctttttcaa ctgctaattt ccttctatct ggcggctact attctttgac gtccgtcatt
     9181 tcaaagtact ttactaataa cttagcataa cgaaaaactg tggatacact cgtatattga
     9241 gcgtctgcat aatcttccaa atttttctca ggatgttgat acatgaattc aatcaaatga
     9301 taattatatg attttctgta gaaatgaaga taaataatgt agattgggaa gttctcctgc
     9361 ttttccatag aaaagcgccg attattctca ttcttcaaaa tgaccttatc aggatatttt
     9421 tggaacaatt cttctaaatc tgttagataa ttcaagatag acttacttga taccttcaat
     9481 tcgttttgta gttcttctat tgaacaccaa cgttctgttt caagcagtaa atgaatcatt
     9541 ttaattttct tttgagaaac ttttgttaaa aaagtctgca tattttctcc tcctgtatct
     9601 actttactat actacataca agaaaaaaaa cggatacaaa tatcatattg tcacaccgtc
     9661 aatagtgcat aaacctgtta ttatttctct ttttttccta agtattctat tgatatgaat
     9721 catttaatcg tttattatat aaatgtcaat aatctaataa taaaataacg tacatatttt
     9781 attattgcat acttaataac agaactatag caaaaaatcc cgtgtatcaa aacgggatta
     9841 gattaagttt cctcaatcac tattcataac acaattatta taacaagtaa gtaaacaatt
     9901 gaaaaggaca tttattgaaa acggttgttt tttacttaag gaggaaattt agtgaaaaaa
     9961 ttagttgcat tgtttttggt cgcaggaagt ttgttgagtg ggtgtggtgc tactacgtct
    10021 actactcaaa gcacaagttc agctcaggct tccagcagca gtaccacgat ctccgaaagt
    10081 acggcagatt catcgaaaga acaagaacaa attgaaaaga aaaaagaaga agagaaacaa
    10141 aaagcggaaa aagctgcact agaaaaaaaa gtgacagaag cggaatctgc tttgtcacaa
    10201 gctgaagcaa atcctacaaa agaaaactat gcaactgctt cagcagctat ccaagcgcta
    10261 cctgaaaaca aacaagagtt gacaagccgt cttgctgcaa ttgattccac aatcaaagca
    10321 aaagaagcag aagaagaacg gaaaaagcaa gaagaaattg cacgacaaca agcagaacaa
    10381 gcgcaacaag aagcaatcgc acagcaacaa gcagaacagg cgcgacagca agcagcagct
    10441 gaacaggcct ctcagcaggc acaagccgca gctcctgagc aaaataatga acaaactgtc
    10501 tatgtcacac cgaccggttc aaaataccat acacataaat gtggaaatgg tacgtatacc
    10561 cctgccactt taagcgaagc tcaagctcgt ggtttaaccc cttgctccaa atgttatgga
    10621 aactaattca aaagttatac tataataata aaattcttct gaaacataaa aaaatagaag
    10681 ctggcgttcc agatagtaaa ccaaaaatag cagtttacac gtccatttcg tcagcttctc
    10741 tcttttttca tttagtctgt tccctctgct tttttggaca tcttcattca ttctttctca
    10801 atcaaatagt acacctcttt ttcttttcgc agtttatatc ttcctttttt caatttcatt
    10861 gaaaactgtt tcgaatgttt atgttttaca tggattttaa gcaggagctc ctcatgttgc
    10921 aagcgatagt tcctgccttt taaatcagaa aatctcacta gtccgtattc atcagaagct
    10981 ttcacaaatg gaactttttc tctagtaata tagtgtttgc cgttgagcgt ttttacagaa
    11041 aagaccattc cttcgattgg tcgtccgttt gtgttcttta cataaaaaga taaaggatag
    11101 tttcgcttag cgatggccaa agaatctaaa taagcgatga gtagccaaac taagccgatt
    11161 gatccaagta gtataccgat aaaccaaata aaaaatttcc attttttcca ttgatcaatt
    11221 ccttggatcg ctttttttgc ctcttttgct tgataaggaa tacgatgccc cgtcacaagc
    11281 agtcgatgtg tattgaccat ataaggagta caggtaagca aagtcacgag atctctgcct
    11341 ttttggatac ccaaagaatc tacttcatca ggtaagacca cttggatttt ttctacttga
    11401 tacgccaatg tctttccatt gatctggatg aaaaactggt ctcctttttt caacttaggc
    11461 aaatctgtaa ataattttgc ttctgggagt ccacgatgcc cagaaagaac ggcatgggtg
    11521 tttttcccgc cagtcggata agaactttct tccaataatg acgttccttt ttgcaaaaat
    11581 atttccgttg tctgatcgaa tataggtaag ctgacattga tttttgggat ttcgatgact
    11641 ccaagcatat gttccataaa aaaggcatta ctaggtaaat cttttaatgc ttttgcatca
    11701 actgcttggt tgaaactgcc tatccctggt gatacgtttt gctctgccaa ctgttggttt
    11761 ttctttgtca ttttttcctg ctgttttttt atttcctctg aattcttatt cgatgcttgc
    11821 ttttgataat ggatgatcat ttgctgggca agaaaatcat tcagtgaatc ttctacaaaa
    11881 ggataagaaa agatcccgat cccaacgact aaaatgattg ccatgaagat gtccagtatt
    11941 cttattctgc ttttattcat catgtccacc tattttctaa tatgtatatg aagaggttgt
    12001 gaaatcagcg ccttgacctg agtattcgct caacaaacgg caaaatagtt cctcctattt
    12061 tgccgtttgt tgagcgaata ccaagagacc gaccacttgt cacaaccttt ctccccattt
    12121 gaatcactat cgtttattta tcgtatgatt tttgtttcca atagtttaag cttctgtttc
    12181 ttgatggcgt tttttgaagt agatcactgc tcctccaact aaagcagtac ctaccaagat
    12241 gaatacgatg atccccatac caccagttga aggcaagatt cctttaggtt ggttatcgat
    12301 cgttgtttca tcagaagttg ttggatcatc atctactgta aattcagtgt acgcagcttc
    12361 attcttaggt aatgcgtagc cttctggtgc ttttgtttct tgaaggcagt atgccccttg
    12421 tttcagccct tcgattgaaa atttcccttc tgaatcagaa atcaatttca cttcttccca
    12481 gtcagaacct gtgacttcat ctgaccaagc atatgggtat tctcctgagg caagatttgc
    12541 tatttttttc attaatttaa cagttggatc ttttgctctt agaatagaaa attctgcatc
    12601 ggctaatttt tcttgcgtgt tcacatcttg tttgatgaag tggaaatcac cgtagttttg
    12661 gacattttct acagtaatcg tgttatccgt agttgtattt tcccgattga ctacaccgtt
    12721 tggattccca agattggctt catctgcgcc atggatgata ccggttgttt gttgcaattt
    12781 tccgtttttt actataaaag tagtttcttt ttgtggattg gttaattgat aaccatctgg
    12841 tgcttttgtt tcaactaaag tataattgcc gtctattaat ccattgacac tgatggatcc
    12901 ttcagtatca gttgtgaatg tcaacgctga ctcagcagga tcatagagag cgacaccagt
    12961 gttttcatca aaaccggtca cgaattgatt ctctgaattt ttgatctgga attgcgcccc
    13021 tgctaactta tctacccctg aaaagttccc agctttctgg attttgatat tgctttcttt
    13081 gatgacattt ttagcatata ggtgaatcgt gtctaaaggt ttgtcagggt ctgatgaatt
    13141 cgggttataa aagggtaagc ttaacacgat atttggcgac gtttcgacac cgggctttgg
    13201 tgtttctaca aagacgtata cagcatcttt cccatcagat ttacctggaa gagtcaggtt
    13261 agctaatcct tcaccagaag tcgttacact tgcgaagggt gtagcgtctg ctggaactgc
    13321 tgtgtcttta cttggatagt acgtagcagt gatgtcatat acgttgaatt caacgccgtt
    13381 caacggttcg ctattcggaa atgtcaattc cgttccgttg tttatttgat tttctggtgc
    13441 tgaagtgaat ttcaatttat gcaaattaac cgtaacattt tctggtgtag gtgatacggc
    13501 ctagaaacct ctgcagttcc taggccgaga acgaaaggaa gcagcatgat agcagttcct
    13561 aaaaacgtcc atatttttgt tttgatgttt ttcattttat ttccctccta ttgtttcctt
    13621 tttgttgttt tttataaaac caagctgtcc ctgttgaaag gactagcaac aaccctaaaa
    13681 ttgagaaacc tccaagcatc tcttcgcttg tttttggaaa gcttcctcca tttttttcat
    13741 ttgatggacc atttgtctgc atctgaactg gcgtttttcc attattcgtc ggatttgctt
    13801 ttggttcgtt gatgatttcc aatggtatag gatgaagcgg atcataactg cctttttcaa
    13861 cttcaaagag aatggttgat tgactcaata tatatccttt aggcggttta atctccacta
    13921 gttcgtaact tccaaaagac aatccatgga cagaaaatgt tccatcttta ctggaagtaa
    13981 aaatcgttgc tcttgaatca tctttggcta cccaatgatt tatcccgttt tcttgcgcta
    14041 agtattggtc attttttttg actagaaatt gagcatctgc tagtttttgt ttcgtttgcc
    14101 gatccacttt gacaaattgt ttgcctcctg tttggatggc aagatgtgtt tctactgttt
    14161 gattgtcagt cgtcaaacgt acatcgttat caaatgtcgt tttttcactg ttttctttca
    14221 atgtcatttg gtaacggatc gttaattttt tcccagcaaa cgaactaaga tcttttccag
    14281 aatctgataa gacaaaacca tgttttgttt tttgtgtgtg aaatgtatga atcttgtctc
    14341 caccaatcaa aaaatcgatc gtttctggtt ctagccataa ctgaggatca gctgtatctt
    14401 tgatcgtata ggttcctatc tcgttgatgt tctcaggaat tgttacggtg atgagataag
    14461 gaactttatc cccatctgca aaatctgtct gcttattctc tactgttttt gttaatgtga
    14521 gtgcagcagg tattttttgt tcgctctttg gatacaagtg gatggtcgtc aatttatgat
    14581 tagatgaatc tgtcacagga agcacaacga caaatggtgc tgatttttct tttacttaat
    14641 ctgaagtctt tatttccgca aatcggtaaa cagcgtcacg accttgctca tctttgtccg
    14701 atgcactaaa agaagcaatg ccctcttcct cgtctactgt ttttgtcaca ttttctgcca
    14761 gtattttttc actatcactt ttttgtgcta gttttctttg tgcgtcttcc acactacttc
    14821 cttctgaccg cagcttgtaa aattcatccg agacatcata tatagcgaaa gtaacgccgt
    14881 tcaacccttt tactccttga aagttatccg ggatatttat tccgtcgttt tgactgatac
    14941 ttggcatctt atcgctttcg aacattcgat tatgcaaagt aaatgtaata ggtgattctc
    15001 tagctgacac agtcggagaa cctataaaaa agaccagtag gataaaaaag agaacagcaa
    15061 atatatccac acgtacattt tttgtttttt tcatcgctgt tcacctgctt ttttcttacg
    15121 atggataaaa taaaagaagc cagaaaaaat aaagatcagt cctaaaaaga caaagggcag
    15181 tagacctatc ccacctgtcg aaggaagcaa gagagtcgga ttgatcgtca acttagcggt
    15241 atctgttgcg ataatcgtat aattattgtt tacacttcct tcgcctaaac taatcgttac
    15301 tatttgtgat ccaatatctg tccggctgtt aactgttagg gtataatcca atgattcgcc
    15361 taagacaaca ccggtaacgt ggagatttcc aaccgaaatc gagtgacttt gggcatcata
    15421 attaaaggca gcgtcatcga cagtaatcac tgcgttagct ttatagatgg tataagttcc
    15481 tgatgtgttg gctatatcag taaatgacaa cttgggattt gctgctttga ctttgtctat
    15541 tccgctattc gttagtttaa ttgtatagct gccagcatta accatatttt ttgctgattg
    15601 tgcatctgct gtgtaatcca tactgctaag tgccacttgt actggagtaa gcgaactagt
    15661 gacaccctct gctagcttga catggattga cgggacacct gcttttccat catatgtctt
    15721 actgccactg agctctaccg caccgctgat ttgcttaatc gtatagacat cagatgtctg
    15781 cacgatagta aataaatagt ttgttctggc agctagttta gctagtccgc tttttgtcaa
    15841 ttgccaagac gtataggttc caatttcagt aggcgcagtt acttcaactc ccttttcatt
    15901 catcgctttg aaatcagttg gattccatgt gatttgatca atactgagat cattgtttaa
    15961 aagggatgtt gattctgttt tatcattaaa ggcatattta acgatcggta caacagaaag
    16021 accatcatag tacttactgc catttgcatt gattgatact gttgcatttg tcttataata
    16081 taggatgact tcagaaatct gttttccatt tgcgtccttt acgattacat ttccatttgt
    16141 atctaaacta gctaaaacac tcattgctga ttggttcttt gtttctgtcg ctgaaacagc
    16201 tgtaaaggta tatccgttga tagtaggcgt attagtcgtc aggtcttgtg cagcacttcc
    16261 gtaagtaaga ttcattattt tagaaggagc aatcatctgt ccaaaatagt cttgatactt
    16321 aatagtgatt ggtgtttgtt tgcctgtata ggtcaccgta tcgacgttac tttctagggc
    16381 tgtgaaagaa ccgtttgttt gtgcaaaaga atagcccgtt actgctgttg gagtgataac
    16441 cactttgtca cccacatgga ttccagataa tgtagtcccg tacgttccga tttttgctgc
    16501 tccaccattg actgtataag aaaaagttcc tgtccgattc gcattgacct gaaccgttgc
    16561 tgcttgatag gttggaaaat ttgaaacatt tcccttacca tcaacgatct gcccattttt
    16621 gatcgagtac cccgtgaatg gaatatcatt tgctaactgt ataccgattg aaaaagtact
    16681 catacctcct ccggcaggaa gccctcccca tgttgagggt gtaaaagaat aacttttatc
    16741 cgcattgggt gtaactgaat cgatcgtatt tccagcggta gccaccccca tatagatact
    16801 ttttgggaat gtgatagtag taccatttac tgtggagcca ataaacagat tatggatatc
    16861 tggacggtta tcaataaaag cgattgcttt ggttacgcct tgtaacgcag ttttatccgc
    16921 actcatcgat ccgttaaccg caacaatgac tccatctttg gcatatatca ctgtatatcc
    16981 atctggtatg gtatgtccgt tgaaattcgc agtggccgca acagtactaa atgtctggct
    17041 tctacctgtt tctgaataag ctttctcccc tgctgctttt gctgctgtga tttctttttc
    17101 tgtcggatca atcagttgag tcgaggatgt ggttgaggtt gtctcgttgt tgctagactg
    17161 attggtgctt tctatggact gtatttcaga atagggtgga ctatttgtct cctctgtcgt
    17221 ttggttagca aaatttgacg tttctgtata aacgtttaca taaatacttg ataagctccc
    17281 taagaaagta aaaatcaaga tgatcgaaat cataatggga tgtaagtatt ttcttttaga
    17341 tacagctttg agtctagaaa caaaatgttt ttgacatata cgttggattt tattttgagt
    17401 tttcttaatc attttcatcc ctcctctcaa taaaaaatca tcaaataatc ctttacacgt
    17461 tattctaaat ttaattttta ttattttatt gatgtataga aagtttgggg ctttttttct
    17521 ctttttattc taaggttatt ttagtcttgt cctttaatac aaagattt
//
LOCUS       DACECY010000057        17005 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.061, whole genome shotgun sequence.
ACCESSION   DACECY010000057 DACECY010000000
VERSION     DACECY010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.061"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..124)
                     /locus_tag="IWO38_002065"
     CDS             complement(<1..124)
                     /locus_tag="IWO38_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAQ0314144.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVF"
     gene            complement(228..539)
                     /locus_tag="IWO38_002066"
     CDS             complement(228..539)
                     /locus_tag="IWO38_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0314145.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(541..738)
                     /locus_tag="IWO38_002067"
     CDS             complement(541..738)
                     /locus_tag="IWO38_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ0314146.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1132..2859)
                     /locus_tag="IWO38_002068"
     CDS             complement(1132..2859)
                     /locus_tag="IWO38_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0314147.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2852..4045)
                     /locus_tag="IWO38_002069"
     CDS             complement(2852..4045)
                     /locus_tag="IWO38_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0314148.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4232..4876)
                     /locus_tag="IWO38_002070"
     CDS             complement(4232..4876)
                     /locus_tag="IWO38_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0314149.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4970..5104)
                     /locus_tag="IWO38_002071"
     CDS             complement(4970..5104)
                     /locus_tag="IWO38_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ0314150.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="IWO38_002072"
     CDS             complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="IWO38_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ0314151.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7235..7708)
                     /locus_tag="IWO38_002073"
     CDS             complement(7235..7708)
                     /locus_tag="IWO38_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ0314152.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8109..8333
                     /gene="nrdH"
                     /locus_tag="IWO38_002074"
     CDS             8109..8333
                     /gene="nrdH"
                     /locus_tag="IWO38_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ0314153.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8492..10651
                     /gene="nrdE"
                     /locus_tag="IWO38_002075"
     CDS             8492..10651
                     /gene="nrdE"
                     /locus_tag="IWO38_002075"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ0314154.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10701..11666
                     /gene="nrdF"
                     /locus_tag="IWO38_002076"
     CDS             10701..11666
                     /gene="nrdF"
                     /locus_tag="IWO38_002076"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ0314155.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11755..12894)
                     /locus_tag="IWO38_002077"
     CDS             complement(11755..12894)
                     /locus_tag="IWO38_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0314156.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13203..13916)
                     /locus_tag="IWO38_002078"
     CDS             complement(13203..13916)
                     /locus_tag="IWO38_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ0314157.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="IWO38_002079"
     CDS             complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="IWO38_002079"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ0314158.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15105..16637
                     /locus_tag="IWO38_002080"
     CDS             15105..16637
                     /locus_tag="IWO38_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0314159.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16828..>17005)
                     /locus_tag="IWO38_002081"
     CDS             complement(16828..>17005)
                     /locus_tag="IWO38_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0314160.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5278 a         3494 c         2909 g         5324 t
ORIGIN      
        1 aaaaaacaca ttccactaaa tttgatgccg gttttccttg aggttttgtt ccagcacctc
       61 ctttaatttt tttaagctct tgatctgaac attgtttagc atttagtcta tttttttctt
      121 ccattacgat tccccctaca aactataaat acttttatgt cactgattca atatacctat
      181 ttttctaatc aaaaaatttt gagacatatt taatattata agtatcatta aaattgagat
      241 ttatctccat aatctgctcg atataaacca tttattccag cagttcttcc aatttcactc
      301 aaatttctta ttaattcctc ttgatattca gtgaatctta ttttattagt ataagcagta
      361 aaatatagat aattcaccaa tctatttatt aaaggactgg gatctaattc atatttttgt
      421 ttttctaatt ttaaatatac cttttgtaaa acctcaagaa tatcagaata tttaggatct
      481 ttacttatag atatatcatt atataattca tgaacaattt gcttagcatt ttttttcatt
      541 ttagcacttc cctggaattg ctccacctaa aaaaccacct atagacattc ctgcaataca
      601 agtagttgcc ttggcccaat cgaccgtaca tttattttta gtgcaataca ctccatttcc
      661 ataatatttt ccactatgag tggtaccccc atagataagt tgtgtctctt taatagacaa
      721 aatttttaaa tgtttcataa tctaatcacc cctaaattaa tgtttttaga ctattattct
      781 aacattcatt aaatttcaaa aaacaattcc ttcttgaaac gtagcaaaat aattcctgaa
      841 taagaattta atcactttta agtatataaa ctatgtatat tctttaatat tttttgagaa
      901 tgcacttcgt acataataaa ctttaatcac attagtcttt catacctacg atcgatctct
      961 ttgactcgca cccataaatt cataatagtt tgtcggcgtc tttgaacgtt tcgaagatga
     1021 gctacgcatt ctcttttttt atagacaaaa aagatagaga cgaacatgga gcttccaaat
     1081 ttggatattt gatccaattt tggaagctcc atttatttcg tgtctgtctt tttatttttt
     1141 ccagctttcg actaattctt ggttctcttt gatccagtct tttgctgctt gctgtggatc
     1201 ttttccattg ttgatttcca acataacttt ttccatatct ttttctgacc aatggaaatt
     1261 gtctaatact ttgtaagctt ctggctggtc ttctttcaat ccttttcgaa ccatcgtatg
     1321 gatctgctct tcgcttccca ttccgttttc aggatcttct aagtatttca gatcatattt
     1381 ggcaaacatc caatgaggtg tccatcctgt aactacaatt ggttcgtgtt ttttgatcgc
     1441 ttgacctagt gcaacggtca ttgctcctga agaagatgtc tctactttcc agtctttcaa
     1501 gttgtcatat ttttggatcg tgttttcagc agccgtaact acaccagctc ctggctcgat
     1561 tcctatgatt ttcttacctg cctgatcggt taactcatcg atcgaattta catccatgta
     1621 ggctggcact gctaaaccaa cttttgctcc tgtcaaattg gcacctaggt cttcgacttg
     1681 atctttatat tgtgcatatt gtgaaccatg agttttaggt agccaagctg aaaccatggc
     1741 atctgactcg ccatttgaaa cagatttcca catgattgaa ttatctaatg gcgtcatcgt
     1801 tacatcatag cccatttgtt tcaatacttc accgactaca tttgttgaag caacttctgt
     1861 atcccattca acataagata agttgatacg ttttgtttct ttggcagatg aaaaactccc
     1921 tataccaatc gcacctaaga tcactactac tacagcacca atgatcagtc cttgacgttt
     1981 tttacttttc tgtgttgttg ctcctgcagc ttttttattg tttggctggt tcaaatgctg
     2041 tgtaaaacgg tcaatgatga ttgctaagat aactaatgca accccgttga caaatccatt
     2101 cccgacttgt gctctttgca atgctgataa tacgccgcgt cctaagccgg gtgcaccgat
     2161 catagaggca ataacgacca ttgaaagtga cagcattgtt gtttgattga tccctgccat
     2221 gatcgtactt ttagctaaag gcaattctag tttgaatagt ttttgacgac ttgtgctccc
     2281 gaatgaatca gaagcttcta ctaattcttt aggaacttgt cgaatcccta aattcgtaaa
     2341 acgaacggtt ggtggcagag caaagatgac tgaggcaaat acccctggaa ccatcccgat
     2401 accgaaaaat gcaactgctg gaatcaaata aacgaatcct ggcattgtct gcataaagtc
     2461 aaggatcggt ttgataatac tttgggcttt actgcttttt gccatcaaga tccctaatgg
     2521 taccccaatg atgatcgaga tcacactaga taaaagaaca agtgtgacag tattcatcaa
     2581 atcattccat agaccttggt tataaataaa ccatagaccg attaatgtga acaacgtaag
     2641 tcctggacgt ttcttagaaa taaagaaagc tgctaccgtc aataacaaga taaacaaagg
     2701 agctggaatc actaacagta aatttgtaat tcccgacatc actgcctgtc caatcgtttg
     2761 taaaaatgaa aataggccag aaaaagttgt cgtaaaccat tctgtgatat tttctaccca
     2821 gtttgccact ggtaattgat agttcaatag attattcatg ttcactcacc tcgcttgttt
     2881 ctgctaatgc ttcaatcaca ctacctcgga tgacgacacc ttttaatttt ccatcctcta
     2941 caactgcaag tggagtcggt gagtcataaa taatattgaa aatatcactt accaccatgt
     3001 ctttcccaat caccgtcaca ttttcatcta ggaagtcgat caatggacgt ttttgcttcc
     3061 tagcttctaa cgcttgatct gcagtgatga tccctttcag gtgtctcttt ctgtcaacag
     3121 ccatcagcat gctgacttct tcattacgca tccttgtcaa tgcaacgttt ggtccatcag
     3181 attcgatatt cgtcgtcaat gcaggtacca tgatgttttg agccgtaagc actttggaac
     3241 gatcgacttc ttccacaaat tcacgtacat aatcatttgc tggattggtc aatatttctt
     3301 cacctgttcc gatctgcatg atttcgccgt ctttcatcaa ggcgattctg tccccaatcc
     3361 gtaaagcttc attcaaatca tgggtaatga aaataatcgt tttttgaaca ttggcttgca
     3421 ggtccagtaa ttcgtcttgc atttctcgcc ggatcagcgg atcaagagcc gagaacgctt
     3481 cgtccatcag caagatctca ggatcatttg ctaaagctct ggctaggcca acccgttgtt
     3541 gcatcccacc agataactga ctaggatatt ggtctttaaa tgaaagcaaa ctagaatttt
     3601 ctaatgcttt ttctgctttt tcttgtcgtt cttctttagg tacaccacgg acttctaacc
     3661 cgtattctgt attttccaag atcgttcgat gtggaaacag accgaagttt tggaaaacca
     3721 tattgatttt atgacgacgg acttctcgca attcttcttt gcttaacttc gatacatctt
     3781 ctccatcaat atagatactg cccgaagttg gctcaatcag acgattcaat agacggatca
     3841 atgtggattt cccactcccg gaaagcccca tgatcacgaa gatttctcct tcttttacgt
     3901 caaaattcac gtcatacaca ccaaccgttg caccggtttt ctttaaaatc tccgttttcg
     3961 gctggtgatc attcatcata tctaaagcgg cttgtgtctt tttaccaaat atctttgtta
     4021 aatgttttac ttctactttt accaaacaaa ttctcctttt ttatgtaaat tcatttttct
     4081 ttctgaaaaa agtgaccaca ctatgttaac atcatgtagt cactttgtca aaaatgaaag
     4141 aaatttgctt gtccaaactg aaaaaaactt ggctaaatct ttgtaaataa aaggtttagc
     4201 caagttaata tttttttaat atttcacaca atcattctgc aagctgatgc tccgggtaaa
     4261 aaaagtttgt catacgctgt agtgtatatt catttccaat aaaatgaacg atgtcccctg
     4321 ccgtaaattt agcatagggg ccaggtgaaa caagcagttc ttcattatga gaaattgcga
     4381 taactgtggc gccagtcgcc tgccagatat tcaactcgct aattgaacgg tccagatgca
     4441 ttgctttttc tgataaagat aattcaaacg gaagtaaagg attcatctta tgtattcgct
     4501 tcgtttgatt gaccagctga tccaataatt gagaaaaatg attcagctca tcttgttgct
     4561 tctccacact tgccatgatt tctgcttttg tatcttggat ggattgtaca tcttgatatt
     4621 gcaataggaa atcttttgct ttttccttcg aagcaacaaa tgccccactt ccatgccgta
     4681 cttccatgat tccaagatct accagcacat tgatagcctt gcgggtagtc tcaggagaag
     4741 cattgtacgt actggcaagt gttgagcggg catggatctt ctcacccact ttatatcggt
     4801 tttcaacgat acgttcggct aaatcaacag caatctgctg gtagcgcggg cggacgattt
     4861 ttttctttcc agacattttc tttcctcttt ctttttttac tttccctcat cttactatgc
     4921 tctagcaaaa taagcaaaaa aggggagaat agcctcttgc aacgactgat catcgcttga
     4981 tctgttcctt ttttactggg caagctgttt gacagtcatc acaattctta ccggatttta
     5041 aacgagtata gacaattgcg cctgcacttc caaagattaa cacacttaat aagaatgtcg
     5101 ccatcctatt cctctccttc taatgacaat atagtataaa actggttctt ggattttggc
     5161 tgacggaaca aaacataaac gagtcctatc atcagtaaaa tagcaagata cgtccctaac
     5221 gctacctgtc caccctccaa taagtgtccc aactgataaa tcacaaaact aactaaataa
     5281 gctaaaccgc attgatagcc tatggcgatc caagtccatt tcttggcatt catttcccga
     5341 tggatagccc caattgccgc aaaacaaggc gcacaaagta aattgaaggc taacaatgaa
     5401 taagctgctg cgggagtata tgtgtgttgt agcacttgcc agatttctct gccattttcg
     5461 gatacttccg catgaccaaa caaaatacca aatgtgccaa taatattttc tttagcgacc
     5521 aatcctgtaa tcgctgcgac tgttccttgc cagtttcccc aaccaagcgg ctgaaaaaca
     5581 actgccagga ttcgcccgaa aaaggctaag atactttggt cttcagaaac aggttgagca
     5641 taaaaattat aagaagagct gaaccagatc acgatactag aaacaaagat aatcgttcct
     5701 gcttttttaa caaaagactt gctgcggtca taggtttgtc ttaggaccgc tgaccattga
     5761 ggaaaatgat atgcgggcaa ttccatgata aaaggagctg tatctccagc aaacaaacgt
     5821 gtttttttca agctgattcc agagaaaata attgcagtca taccaagaaa ataggcagaa
     5881 ggagctaccc aagattgatg aggaaaaaag gcccctgcta ctaaggcaat aatcggcaac
     5941 tttgcagaac aaggcatgaa ggttgtgacc atgatcgtca aacgacgatc ttgctcgttt
     6001 tcaatcgtgc gactggccat gacgcctggc actccacatc ctgtagcaat cagcatagga
     6061 atgaaggatt ttcctgaaag accgaatttc ctgaaaagtc gatccatgac aaaagcgatc
     6121 ctcgccatat atccgcagtc ttccagaaag ctcaagcata aaaacaatat aatcaattgc
     6181 ggtagaaatc ctaacactgc tcccacgcct gcgatgatgc cgttcaagat cagctcctgc
     6241 atccatcccg ctacttgcca ttcagctaac aatctgccaa catgttctgg taaccatgta
     6301 ccaaacaatg tgtcattgat ccaatctgtc cccattgttc ctactgtttg aatagccaga
     6361 taatagatca gccacatcac aaatgcgaag atcggcaagg ctaaccaacg attcgttgca
     6421 atctgatcga ttttgtcact catcgataat tggaaagaat catttgacac aacacagagt
     6481 gtacatagtt gggtaataaa ttcataacgt tcattgacca gaatcgtctc actatcgtcg
     6541 tccaatagtt tctctgttaa ctggatcagt tgctcgatct ctttttgctg agaaactgct
     6601 atctcgtaat cctctcttac cttctgatct cgttcaaaat atttcattgc ataccagcgt
     6661 ttcttgccgc tttgttctcc tagtacatct tcaatttcat gtaaagctgc ttctaaacgt
     6721 ggatcatatg tacagggaac aggaatagaa ggccgctttt ttgcagactc ctgaatcttt
     6781 tcgattagtt tttcgattcc ttgctttttc aatacgctga ttggtaaaac tggtacacca
     6841 agaccatagg aaagcttttc tagattgatt ttttttcctt cttttttgat gacatccatc
     6901 atattcagac caatgatcat aggaatgtct aaatccaata actgcgtagt taaataaaga
     6961 tttcgttcta aattggtagc atcaataatg ttgatgatca gttcaggctg ttcctgaacc
     7021 aaataatctc ttgcaatcac ttcttctggt gtataaggag acaaagaata gatgccgggt
     7081 aggtcctgaa tatgaatgtt cggatttttt ttcaacgatc cttctttttt ttctactgtt
     7141 acgcccggcc aattccctac atattgattc gaaccagtca agagattgaa caagctagtt
     7201 ttcccgctgt ttggatttcc gactaacgcg atattcattc tttttcctcc ctttgtatca
     7261 agattagttc ggcctctgac tttcgtaacg tcagttcata ccctcgcact gtcagttcga
     7321 ttggatctcc cagaggggct agttttcgga taactatttt tacatttcgc gtcaatccca
     7381 tatccattaa ccgtcgcttc actgcgcctt gtccgtggat tgctacgacg atcccagtct
     7441 ctcctgcttt taattgatca agagaagtcc agctttcaga ggaagcagtc tttttattga
     7501 caaagatttg acctagtatt tcccgactta ctgccactcg atgacttttt accagtaaaa
     7561 tggcattgtt gcctgataaa ttgacgacga cgattttact tccggcaacg atcccgatat
     7621 tttgcaaatg gcgttttgtt ttttcttctg tttcgattcg ttcgacttga taaacgaaat
     7681 tctgctttgc ctgcgctaag gtctgcatat tgattcctct cttgtgaaat aaatcatgta
     7741 ttaaaatcat tcattatatg ttctaatttt cttacgtgtt acgcaaataa atgagaatca
     7801 ttctcacata gaatactctc ggtaattgag aatgtcaatt atacttgtga tattatgagc
     7861 gaatctttag tattgataga taaaaatgag aaaaaaatat ttcttcatag ttttccacaa
     7921 ttttttctct attaaatttg ctattttccc acacgatact tgttttcagc tcttttttca
     7981 aaaacacaaa acttagtatt ccacagtttc cacaacacaa tatgtagtgt ttgttgtttg
     8041 acttttttac taagagtttt atactatcta ttatagataa taagaatgtg ttagaggagg
     8101 cactcaccat gaacgtaaaa atcttttcta aaaataattg tatgcaatgt aagatggtca
     8161 aacggttttt aacagaaaat caaattgctt ttgaagaagt caatatcgat gaacagcctg
     8221 aagcagtaga ctggttgaaa gcacaaggat tccaaagtgt tcctgtcatt acttctgatg
     8281 cagaaacagt tatcggattc cgtcctgatc agttgcgtaa acttgccagc taacatgact
     8341 aaaagaatca aaacgtgata catggatttg aagggcttga gacagcacca gtttttgtcg
     8401 caaacttcca aatccttttt taaagcttaa aaacaatgca atgatttgca atcaagcatc
     8461 atcaaatacg aaatgaaggt tgggcgtacg tatgagtctt aaaaatttac aagatgtcag
     8521 ttatttcaag ctgaataatg aaatcaatcg gccagtcaat ggtcaaatcc ctttgaataa
     8581 ggataaagaa gctttagttg ccttttttga ggaaaatgtc aaaccaaata cgatgacttt
     8641 tccatctatt atggaaaaaa tcaattactt gatcgatcaa gaatatatag aagcagaatt
     8701 cattcaattg tattcaccat catttattga aaagctatat caatttttgg acgagcaaca
     8761 tttttctttc aaatctttta tggctgctta caaattttat tctcaatatg cgttgaaaac
     8821 caacgacggt tccgcttatc tggaaactta tgaagatcga gtagccttca atgcgcttta
     8881 ctttgcgaac ggggatgaag aattagcact tgctcttgca gacgaaatga tccatcaacg
     8941 atatcagcca gctacacctt ctttcttgaa tgctggacgt aaacgtcggg gcgaattggt
     9001 gtcttgtttc ttgactcaag tcactgacga tatgaactct atcgggcgct caatcaactc
     9061 tgctttgcaa ttgtcccgta ttggtggcgg cgtagggatt actttatcaa atcttcgtga
     9121 agccggcgct cctatcaaag gatatgaggg tgctgcaagt ggtgtcgttc cagtcatgaa
     9181 attattcgaa gatagtttca gctattctaa tcaattaggt cagcgtcaag gagctggcgt
     9241 ggtttatttg aacgttttcc atccagacat tattgctttc ctttcagcaa aaaaagaaaa
     9301 tgcagatgaa aagattcgcg taaaaactct ttctcttggc gtgatcgtac ctgataaatt
     9361 ttatgaattg acacgtaaaa acgaagatat gtatttattt agtccatata gtgtcgaaaa
     9421 agaatatggt gtcccattct cttatgtaaa tattacagct gagtatgatc atctagttgc
     9481 aaatccaaac attcgcaaac aaaaaatcaa agcgcgtgat cttgaaaatg aaatctcaaa
     9541 attacaacaa gaatctggct atccttacgt aatcaatatt gatacagcga accgcgagaa
     9601 tccaatcgat ggcaagatca tcatgagtaa tttatgttca gagattttac aagttcaaac
     9661 gccttctgtg attaacggca aacaggaata tgaagtatta ggaacagata tctcatgtaa
     9721 cttaggctcg acaaatatcg tgaatttgat gcaaagccct aactttggtc aatctgtgcg
     9781 tacgatgact cgtgcattga catttattac agatgcatct gatatcgatg tcgtaccacc
     9841 gatccaaaac ggaaataagc tgaatcatac gattggatta ggtgccatgg gcttacacac
     9901 ttatcttgca aaagaacaaa tggaatatgg ctcagaggat tcattagact ttacaaatat
     9961 ttacttcatg ctgttgaatt attggacact tgttgaaagc aacaatatcg ctcgtgaaag
    10021 aaaacaagtt ttccataatt ttgaaaaatc agcttatgct gacggcactt atttcgaaaa
    10081 atacgtaact ggagaattcc agccaaaatc agaaaaagtc aaagcattgt ttgaaggcat
    10141 ttttatccca actgctgaag attggaatgc tttgaaacaa gcagtaatga aagatggact
    10201 gtatcatcaa aatcgcttgg cagtagcacc aaatggctca atttcatata tcaacgatac
    10261 gagtgcaagt attcatccta tcactcgcat gatcgaagaa cgccaagaga agaaaatcgg
    10321 taaaatttat tacccagcgc cttatctatc caatgagacg attccgtatt acacttctgc
    10381 ttatgatatg gacatgcgta aagtaatcga tgtttatgca acggcacaac aacacgtcga
    10441 tcaaggcatg agtttgactt tattcatgcg ttctgaaatc ccagaaggca tttatgaatg
    10501 gaagacaaca acgaagcaaa cgacacgtga tttgaatatt ttaagacatt acgctttcca
    10561 caaaggaatc aaatccattt attacgtacg tacctttact gatgatgcag aagaaatcgg
    10621 aagcaatcaa tgtgaaagct gcgtgatcta atatcataga tagattcgct tattcatatt
    10681 ttaattaaag gagcattctc atgtcagaaa catattatga agcaataaac tggaatgaaa
    10741 ttgaagatat cattgataaa tccacttggg agaagctgac agagcaattt tggctagata
    10801 cacgtatccc actttcaaat gacctagatg attggcgtac attgtctcaa ttggaaaaag
    10861 atacagtagg acatgtattt ggcggtttga cactgctaga tactgtccaa tctgaaagtg
    10921 gcatggatca gttgcgtaaa gatgtccgca ctccccatga agaagccgtt ttgaacaata
    10981 ttcaattcat ggaatccgtt catgccaaaa gttattcttc tatttttagt acactgaata
    11041 cgaaaaaaga aatcgaagaa atttttgact ggacaaatac gaatccttat ctgcaaaaaa
    11101 aagctgagcg tatcaatgaa atctacaaac acggtacacc gctagaaaag aaaattgcca
    11161 gcgtcttttt agaaacattc ttgttctatt caggttttta tactcctctt tattatttag
    11221 gaaacaacaa attggctaac gtagcagaga tcatcaaact aatcatccga gatgaatctg
    11281 tacatggaac ctacatcggt tacaaattcc aattaggatt caacgagtta cctgaagcag
    11341 aacaagaaac gttaaaagac tggatgtaca atcttcttta tgagctttac gaaaacgaag
    11401 aacgttatac agaagaatta tacgatccaa tcggctggac agaagaagtc aagacattcc
    11461 tacgctataa tgccaacaaa gcattgatga acctagggat ggatccactc tttccagata
    11521 ctgccaatga tgtcaaccca atcgtaatga acgggatttc aacaggcaca agcaaccatg
    11581 acttcttctc acaagtcgga aacggctatt tgttaggtca tgtagaagcg atgaaagacg
    11641 acgattattt gattggattg gaataaggat ttattttaat tcaaaaattt ttcgaaaaat
    11701 aaaaagagtt tagcgtacag atattttaaa tgtacactga actctttttt atgcttattc
    11761 acttgtttct tccagcactt ttggactgtc taacttttcc gaaacatgtt ccattcttct
    11821 caataaatag attttccgga tatttaatat tatcgtctta atcactgcat agacaggtat
    11881 gcaaatcagc atgcctaata ttcctgcgac acttcctgct ccaatcaata ccaagatgat
    11941 cgtcaaagga tggatattca ttgatttccc aaataacaac ggcttgatca aatttccatc
    12001 taattgttgg atcactaaaa tcgacaacgc catatacaaa gcttgaaatg gagagataaa
    12061 caaaccgaca atgattgctg gtaccgctcc aataaaaggg ccgacatacg ggataatatt
    12121 cgtgattccg caaatgaccg caagcaacaa gctataaggc tgatcgaata ttctcattgc
    12181 caaaaagctc attacaccaa taatcaaagc atctaagatc gtactgctga tataggcaga
    12241 taatgtttcg tttaattctt ttaccgtttg gcgtaactct ttacgaatcg ctttcggaaa
    12301 gaagtgagac aacgcatcaa taaatttgtg cccatccttg aacataaata gcaaaataaa
    12361 tggaacggta aataacaaaa taaaaaattg catcataaat gaaatgattt ttgagacact
    12421 tgcagataaa ctgactaata tgatattgaa caaattacta agagataaat ttgctgatgc
    12481 taattgttct tccaaattga aatttttaaa ctcttcccgc tgcgccaatt catttaacca
    12541 tttgcttgtt tcatctgcat acattggcaa gctctgggtc aattgaattg tctgttccat
    12601 taactgaggc actacattca tgacagttaa aacaactaaa atgattacga ccaaaaacgt
    12661 caataaaaaa ccgaacactc tcgggatccg atgcttttcg agaaaaacaa cgattggatc
    12721 gaacatataa tagataaaac cagcaacaag caatggtaaa aaaattgccg aaagcatttt
    12781 caatattgga ctaaaaatat tcggcatttg taaaataaag tacacgccga tgatcgtgag
    12841 tactagctcc actgtccaga aaaataattt tgattgttta aaacgtgatt gcatacatct
    12901 cctccaaggt cctttgatac ttctattcta tcatttaaca aagagttacg cgctgagatt
    12961 ttcatttgct taaaaaaaac aaaaaaaata aacatatttt aatgagtaga cgagtactta
    13021 aacaaaaaca cggtttggag tagtgacaaa aattatactg cagtcccttt ttccaaataa
    13081 acggtgttca aaagcttgca tcttcgtaat aagcccagcc aaacgaaaaa tataaggaac
    13141 tatttttatt tggctgggct tattacttga tgtaggacat ctattttaca acctcagatc
    13201 atttattgcg caatactgac taaatgcaac tttgtgatat ttttgtgtac ggacaactcc
    13261 tcgatcacat cggcataagt gagccctttt ggaagatcaa tcgtatagat atttttgtac
    13321 actcggtagt catcaacgaa ttttacgtca aaattcacat cttcgattac gatatttttc
    13381 tcttcaaaat aatcggtaag aaaatctttg gtttcttttc tatggataaa ttggatttct
    13441 aactttttag tcgtaggtac atggatcacg cgtttcacta ctgtgagtgc aatagcaatc
    13501 ccgataaaac tgatgatcgc aatggaatag taacccatgc cgatggcgat ccctaatcca
    13561 gcaattgccc ataaagaagc ggcagtcgta agtcccgtca ccgattgttt cgtgacgatg
    13621 atcgttcccg cgcctaagaa accgacacca ctcaccactt gagcaatcag ccttgcttca
    13681 tctgaacgaa tcgttccagc aagctgtgga tactctaaag catctcttaa tgcaccacta
    13741 gcgatctcaa cttggatcaa tgcaatgata catgctccca tacaaactaa aatatgcgta
    13801 cgcatacctg ctggacgatt tttatattgt cgttcaaaac cgattgctcc gccaaatagc
    13861 atagcgatcc ctaaacgtaa aataatctct ggaattgtca ataccatatc attcatctaa
    13921 acaccctcca gtccaatata tcatgtctga aaggagcaga caaaaaaagt gcaattttta
    13981 cctcttacga aaaaaggact gtgacaaaat ttttgtcata gtccttttta tgaataaacg
    14041 gtgttcaatc agctgaccct cggtattact caacaaacgg caaaatatgg ggagctattt
    14101 ttcctttgtc ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttagcctac
    14161 tattgaaaat tacaacttac ttgctgcttc ttcatcgatg atcacaacaa cattgtcgtg
    14221 attttgcaat acacttgctg gcaaatgatt cgttacaggt ccttcaatca tccctttgat
    14281 tgcatcagct ttgctttcgc caaatgccat caagacgatt tctttacttt tcataattga
    14341 accgattccc attgaaacag cttttgttgg tacatcttct gctttatcga agaatcgttt
    14401 gtttgcattg attgttgatt ctgttaagtc aactactgaa gtcgttccgt caaatgaagc
    14461 accaggttca ttgaaaccga tatgtccatt ttgcccgatt cctaagattt ggatatcgat
    14521 tggatgtgca tcgatgattt gatcatactt tttgcatgct gcttccagat cttctgcttt
    14581 tccatctggt acaaacgttt ctttgaatgg ttttttatca aataattgga catccatgaa
    14641 atgacggtag ctttgttcat ctgaaccgcc tagtccaacg tactcatcta agtttacaga
    14701 agtcatttca ctgaaatcca aatcgcttga agtcatttct ttatataaag taactggtgt
    14761 gcttcctgtt gccaatccta atacttttgc acctttgttc ataccttctt tgatgatttc
    14821 aaatgctttt tttccgcctt cttcagcgtt ttttacacga atgatttcca tagtgattgc
    14881 tcctttatat tttggtatag cccaattata gcattctttt tttcttttta caataattag
    14941 tatagaccaa attgcatttt tttcattttc ttgacaataa ataggaatcc gtgttaggtt
    15001 tcataagatg gttcaatcaa acaatccatg ttgcatgaaa accaagcaag tgattcactg
    15061 tggacgcttt atgtctgcag tttttttatg aaaaaggagt tattatgagt atactgacat
    15121 taaacaacat tacccagcaa tttggcgaca aaatcctcta tgaaggtgtc gatctgcaat
    15181 taaatgctgg cgaacatctt gggctgattg gacaaaacgg agccggtaaa agtacattga
    15241 ttaaaatcat cactggtgag atcttaccag atgacggaca gatccattgg caaaaaaata
    15301 ttcacaaagg ttacctcgat caatatgtgg aagtcgatga aacattaaca atcgaagaat
    15361 ttctaaaaac tgcgtacgca aaagaatacg aaaaggaaga aaaaattgct tttctttacc
    15421 aaacatatag cgagaccttc gatgattcct tgctggaaaa agctgggaaa ctccaaacgg
    15481 aattagacca aggagtgttt tatcaaatcg acaccttagt tgctgagatg agcagcgggt
    15541 tggggatcga tgtcttaggt ttagattcac tgttgaaaaa tctaagtggt ggacaacgtt
    15601 caaaggtgat tttagcaaaa ctacttttag aaaacccaga tgttctgatc ttagatgaac
    15661 caaccaatca cttagatgat cagcatatcc aatggctgac gcagtttctt caggattttg
    15721 acggtgcgta tatcgtgatt tcccatgacc aagaatttct tgatcagatc accacccata
    15781 tcgcagacat cgagtttgga aagattacta agtatactgg ccatctaaaa caagcattga
    15841 aacaaaagga acaaaatcgc gaaagctatt tgcgccaata tcatgctcaa caaaaacaaa
    15901 tcgaaaagac cgaagcttat attcgaaaat acaaagccgg ttcccgatca acgatggcaa
    15961 aaagccgaca aaaacaatta gataaaatcg aacggctgac gcctccagct tcagcaagta
    16021 aagctgtctt tgattttcca ttctcgccta ttgtcacgac attagcagtg gaaacaactg
    16081 atttagtcat cggatatgat cgaccgctac ttgcacccat caatttgacc atccgctttg
    16141 gtgaaaatat tgccattcga ggcttcaacg ggattggaaa atctacttta ttaaaaacac
    16201 tgatcggtga gattgaaaaa ataagtggcg atttccactt cccagataac acaaaaatca
    16261 attacttttc acaagatctg tactgggaaa atccattaga aacaccacta caatatttaa
    16321 gcagtcaatt tccaaaagcg actatcaaag aattacgtcg ccaactagct aaagcagggt
    16381 tagttaatca attagcaagt gaacctctca ctacattaag tggtggggaa caaactaagg
    16441 tgaaacttgc acagttgaca atgaaccaag gtaatttact gattctcgat gaaccgacga
    16501 accatatcga tcaagaaacg aaagaaagct tacaagacgg gatacagaaa tttccaggaa
    16561 cagtaatcat cgtctctcat gaacaagaat tttatcaaga tcttgtgcaa cgagtcattg
    16621 agattgaacc aaaataaacg tttgattgaa tagttgaaaa aaaggagctg tgacaaaagt
    16681 tttgtcacaa ctccttcttc tgaataaatg gtggccaatt agctgacctt cggtattagc
    16741 tcaaaaaacg gcaaaatatg aggagctatt tttatttggc tattcttatt acttgatgca
    16801 gaacagcttt tcacaacctc ttttcgtcta ttttttattt tcgagatttc tctttccttt
    16861 aaaaacaact gtttgaaaga agaacgcaca tccttgaaaa acaagcccgc taaccgttac
    16921 acctaaccag ccatactgct gccacattgt cgtccctagc aatgaaccag ccgcacctcc
    16981 aataaagtac ataaacataa ataca
//
LOCUS       DACECY010000058        16145 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.062, whole genome shotgun sequence.
ACCESSION   DACECY010000058 DACECY010000000
VERSION     DACECY010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.062"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..402
                     /gene="scm"
                     /locus_tag="IWO38_002082"
     CDS             <1..402
                     /gene="scm"
                     /locus_tag="IWO38_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ0314161.1"
                     /translation="SSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSS
                     DSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTG
                     SQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(557..1543)
                     /locus_tag="IWO38_002083"
     CDS             complement(557..1543)
                     /locus_tag="IWO38_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ0314162.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1566..2132)
                     /locus_tag="IWO38_002084"
     CDS             complement(1566..2132)
                     /locus_tag="IWO38_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ0314163.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2146..2562)
                     /locus_tag="IWO38_002085"
     CDS             complement(2146..2562)
                     /locus_tag="IWO38_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ0314164.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3019..4953)
                     /locus_tag="IWO38_002086"
     CDS             complement(3019..4953)
                     /locus_tag="IWO38_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0314165.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5255..6190
                     /locus_tag="IWO38_002087"
     CDS             5255..6190
                     /locus_tag="IWO38_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ0314166.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6280..7383
                     /locus_tag="IWO38_002088"
     CDS             6280..7383
                     /locus_tag="IWO38_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ0314167.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7396..8349
                     /gene="menA"
                     /locus_tag="IWO38_002089"
     CDS             7396..8349
                     /gene="menA"
                     /locus_tag="IWO38_002089"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ0314168.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9163..10509)
                     /gene="gor"
                     /locus_tag="IWO38_002090"
     CDS             complement(9163..10509)
                     /gene="gor"
                     /locus_tag="IWO38_002090"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ0314169.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10643..11884)
                     /locus_tag="IWO38_002091"
     CDS             complement(10643..11884)
                     /locus_tag="IWO38_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ0314170.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(11902..12825)
                     /locus_tag="IWO38_002092"
     CDS             complement(11902..12825)
                     /locus_tag="IWO38_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ0314171.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13046..15538)
                     /locus_tag="IWO38_002093"
     CDS             complement(13046..15538)
                     /locus_tag="IWO38_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ0314172.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15545..16012)
                     /locus_tag="IWO38_002094"
     CDS             complement(15545..16012)
                     /locus_tag="IWO38_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ0314173.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4741 a         3370 c         2871 g         5163 t
ORIGIN      
        1 tcaagtgaca gtacagatac tacctctacc acaagtgata gctcagattc atctaccact
       61 acaagtgata gcacggacac gtcagcttca agtgacagta cagatacatc ctctaccaca
      121 agtgatagct cagattcatc aacttcaagc gatagtacag acacgtcgtc taccacaagc
      181 gatacacgtc atgcaagtgt ctccttctca aaaacaagtt cggataacag gacgaacttt
      241 aacttaacta ataaaacaag caatgacaat ggaaacaaat caaaaagagc acttcctaaa
      301 actggaagtc aatcgaataa ttggataaca ctcgcaggcg ttatattact tgtaatcggt
      361 ttaagaatca cttttatgag ttataagaaa tcgaaaaggt aaaaaaatat tcaagataaa
      421 aaagagcaac gtcgctaacg gacgttgctc tttatttttg ctttttatca taaatacaag
      481 aactacaatt caatttgtca aaaaacacgt agatttcctt ctgagaaatt tatttacgta
      541 tcgaaccttt ccctattcag tctttgcgat ccaaaatcag ttgggtaagg gaaaagagat
      601 tctcttttgt tcgaagagag gtggcaggta atttctttat ttccttcaaa gccttttctg
      661 tgtaagaggc cgctaacttc ctagcttctt ccacgcctcc tgtatgttca acgattttgc
      721 gtacggcgtc gacatcttct tctgtcattg ccgctttctt ctctaaataa ggtagtagct
      781 ttggtttgtt tttttgcaaa gagtagatca acggtaaaga atagacccct tgacgcatgt
      841 cttctagtac aggtttgccg atttcctggc tctcttttgt ataatctaaa atatcatcga
      901 taatctgaaa ggccatcccg atagaaagtc cgatatctcc tgcttttttt gcgaagcgct
      961 cgcttgttcc actttcaaaa gcgccaacag aacaacttaa ttggaacagt tcagctgtct
     1021 tacctgaaat attttccaaa tattctttta cagaaagttc ataattatag cgatcatcca
     1081 tctgccctaa ttcgccattc aatacttttt ccatactgcg agaattcaat tggagacttt
     1141 tcaaggaagt ggagtaatca gacaatagtt taaaacagca gacaaataaa tagtctcctg
     1201 catagacaga aacgacatta ccaaactgac tacggatatt aggctgacca cgtcgcagat
     1261 ccgcttcatc aacgatatca tcatggatca gtgtggccgt atggagcatt tcgatagaag
     1321 ctgccaaagc gatggctttg tctcgatctt gctctggtcc aaattgtgag aacaatagct
     1381 gataggcagg acgcaacagc tttccgccgg catggatcgt ttctttgatg gcttgttcga
     1441 caggtttgtt tttcaggcgg acagtttctt ccatccgttt tagtgtttct gtcaattctt
     1501 tacttaaagc aggatagttt tcccacattg gatgtagctt cataaaaaaa ctccttgtgt
     1561 gacgattatt ttttggtaaa cattgggctc catttttgtt tggttccctg ctcataatct
     1621 ttttggaatc gttgaagtgt ctgcacatgt cgtaaaagat aattcgctgc gatcccgatt
     1681 gcaatccctg ataaaatccc taaaactgaa aggactggca agtacaacat gactgtccat
     1741 gattgtgcaa tgaaagaagc catcaaaagc tgtccgacat tatgcatgaa accgccggcc
     1801 gcactaattc cgatgatact gacacgtttt ggtcctaatt gcttgaccaa taacattcca
     1861 aaataactaa gcaaagcacc actcatacta taaaggaatg tggaaatcgt tccgcctagc
     1921 aaagtagtca ggaatagtcg tagccaaagt agtaagaagc tgtcttgttt tttcatggta
     1981 aaaatggcaa cgatcgtgat tagattggct aagcctagtt ttgcaccagg cgcaaaagaa
     2041 aaaggataag ggatcatatt ttccaataaa ccaatgatca ctccttgtgc aacaagtagc
     2101 gaaatataaa tcattttttg taatttactc atggtgtgtg aaggtctaat aaatcagact
     2161 gtcttcgtca cctccatctg ttgaacggac ttcgatcact aatttatgtg gcaaacagac
     2221 gatggtttcg ccatttttcg ttgcccagcc tcgacgtacg cagatctggt catcgcaatc
     2281 tgcttctttt atccgtactc gatcgccatc tatctcgatc aagttataat ctccgtgttc
     2341 atctttgtaa gtatatgtgt tgctctcttc tccagccatc aaagggaatg ttttgatttc
     2401 tgtgccatcc acacgcagta ccgcttcttt ttctgctgtt gcggtgtttt gctgaagacc
     2461 aaaaatcatg actggtaaaa aagaaagaac agtcaaaagc aaaataataa aaacatccca
     2521 aggtttgagc agactttttt cagcaaattc tttaaggctc atttttgttc tcctttttcc
     2581 agattaactc acccaatttt agcataaaaa caagaggttg tgacacctgt gtcttgcttt
     2641 gagtaataag aacgaagaat agaaaacagc tttccatgtt ttctaattct ttgggcttat
     2701 taccgagaag cagcaggtgg aacaccgttt ataacaagag gttgtgacag aagtgggcaa
     2761 cttcaagaaa taagaaggaa tttacgaaaa ttacttttca agtttttgtg aaatttcggc
     2821 ttatttttcg aagaagctac ttctggaaca ccgtttataa caagaggttg taacagaggt
     2881 tgtgacacct gtgtcttgct catggaggct tatctcgggc atcttattcc tttaatttta
     2941 cataaaaagg aggctgtgac ggtttgtctc agcccctttc tattgaaatc ttgcattcaa
     3001 ataacgttct ttatctattt atttcttgcc gtactgggaa cttggtctac cgtaccacca
     3061 attacctagt gtacgttgta gccatggaat tacccagcga tccaacccaa tcgcacgtcc
     3121 ggaaccgttc atcaatgcga aagcaacaaa aatgaaccaa atgtttaccc agtaaaacat
     3181 gcctgataag cagaacgtga tcaccaatac aatcgttgtc gcactgcata gccatgtgaa
     3241 taatccagca atcaatgcta atgccaaggc aatttcaaag aataccatga atttttggaa
     3301 gaacattgct acatcattgt ttggcatgat cacttgcatg atctttgtga accagtctgg
     3361 tgacttctca atcacagcca ttggttcttc accgtatgca taactcaaac caaaatgtgc
     3421 agcttgagtc gctgcttctc cggcacttgc ccctgctccg ctggcagctg tggttgcatc
     3481 accagcagca gaagcaccac tggcaacatc tgttacattg tattcccatg tccaagggaa
     3541 cacactgttt ccagtgaacc atgatccttc accaaaccaa gtacttggtt tgaacacttc
     3601 accattccca atgatttttt tggaagcttc aacgaaccaa actaaaccat agaaaatacg
     3661 aagtggcaca ctccataaga cattgccgta acgagaagaa tgtcctcgag tcacgttgcg
     3721 atcatctttg atatggaaaa actcatgcat taaatattgg aacatataat aaccagaacg
     3781 aatatcaaag aagtatttca aattcacgat atgtttcatt acgatagcta aaaatccgct
     3841 taaatggatc ttatcaaata ggttagctac cccccatttg gcaccaatag aaaccataaa
     3901 tccttgatag tttcctttaa atttatgctt ttctccgcct ttgatcgaag ccacgatatt
     3961 cgctgcggct gtgtgtcctg tttgttcagc agcttgaacg atctgcggtg tcggtgttcc
     4021 tgggaattct tcataaaaga ctaaatcacc aatgatatag atgtttttat cttcgtagcc
     4081 ttttgcctgc atatattcat tggcgatcaa ccgctgtccg cgtgctgatt ctaatccaaa
     4141 gtcagcagcg tcagaagttc ctttgacacc agctgtccaa atcagtgtgt gagtcggaat
     4201 gttgcgtccg tctttcaatt tgatatgatc agcagccact tctacgattg gtgcattcaa
     4261 taataattcc acgttgtgct tacgcaaata gcgttcagct tttgcagcat catttcgatc
     4321 caacatattc aagatcgtcg gcattgcctc tacgaccatc agtgtgattt cgtttggatc
     4381 taatttataa tctttggcta ggcgattttt ccagtcgatc agttccccaa tcatttcaat
     4441 accggtaaat ccagaaccac agacaacgaa agtcagcatg gctttacgca gttcatcatc
     4501 tggttccaat gcagcttttg ctactgtctt ctcaatatgt tcccgaatcc ttacagaatc
     4561 ttcaaatgac caaagggtga atccgtgtgc tttgacacca ggtgtaccaa agtcattcgg
     4621 ctctccgccc attccaagga tcagctgatc aaaaggatag gaacctaatt tggtcttgac
     4681 gatttttcgt tctttatcga taccgattac agtgtctgtg acaagttgta cattcttttg
     4741 acgagcaaac aaatgctgca gatcgtattg gattgcttct ggttctacac gtccgccagc
     4801 tacttcatgc aactcggtca tcatagtatg gtacgaatga cgatcgatca gagtaattgc
     4861 tacatcttct tttttcagtt tctttgccaa gtattttgta gctgaaacac cggcatatcc
     4921 tgctcctaca acgacaatat tttgctttgc cattgatcat ccgctcctta tagactatct
     4981 atataatgaa tatatgataa ttcttttagt ttgatactta ttacacaatc tccatatatt
     5041 atatagatgt tctgttcttt tgtctaactc cttttggatt tttttcaaat tttaaaataa
     5101 agcgtttttt cacgaaattt gctgaaagaa aacgtttgaa atattacatg ggttatttat
     5161 caaaaatagt tgacttatag gctaaaaaga ttagaatgta taatgaatca caaagaaaaa
     5221 ttgtggacta gaaatggaat ggtggggtat taaaatgaag aaaaaacgtt ttgtaactgg
     5281 atttgccgta ttagcatttt cagctctagt gttaggcgct tgcggcgcag atgacaacgg
     5341 aagctcaaac tcttcttctg aatcatctac agcacaatct tcgactgcaa aaagcacaac
     5401 agaatcatct gcaaaagttg tagctggcgg agatttgcaa gacggcacat ataaattaga
     5461 agaaaaaaat tattcaaatg gttaccgtgc agtctttgaa atggttgtaa aagacggcaa
     5521 aatcactgaa tcaaaatatg ataacgtcaa tgaaaacggc gaatcaaaaa caaaagatgc
     5581 agaatacaac aaaaacatgg aagcaaaatc tggcacaaac ccagaaaaat acattcctga
     5641 gttgaatgac caattcttaa aagcacaaag cgcaagcggt gtggaagtcg taactggagc
     5701 tactcactca tctgaaagct tccaaaacta cgcacaacaa ttgatccaag ctgctcaagc
     5761 tggtaacaca gatacaatcg aaatcgacaa tggtgctgat ttgaaagacg gcacttacaa
     5821 gcttgaagaa aagaactact ctaacggcta ccgtgtgcaa ttcgaaatga cagttgctgg
     5881 cggcaaagta actgaatcaa actttgatta tatcgacaaa gacggtaaat caaaacaaga
     5941 tgatacagaa tataatgaaa atatgaaagc aaaaagcggt acagaaccaa aaacttacat
     6001 ccctactctg aacgacgaat tcgtcaaagc aatgggcgaa gaagatggct cacctgctaa
     6061 tgtagaagtt gttacaggtg ctacacacag ctcacattca ttcatcatgt acgcacaaca
     6121 attagtgaat gcagctgaaa aaggcgacac tcaaacaatc gaagtagata atatcgtaac
     6181 agaaaaataa tcacataatc atactaataa aagttggagg gtgggacgat agccagatta
     6241 ggcagtcgtc tcacctttca aaatgaagag aggaaaaaga tgcggaaaaa actactattt
     6301 tccttaatca cactgggcat ctcttttatg gtgctaactg gctgcagcaa ctcagcaaat
     6361 aaaacaacca ctgaaagttc agcagctacc aagatattga aagatccata ttctgatgaa
     6421 cagttcttgt tagggactta cgttcgtatc cgtgtgtatg atgaaggcaa agaatcagca
     6481 ttgaaacctg ccttcgatcg agtaaaagaa cttggtgata aaatcacgat caatcaaaaa
     6541 ggttctgaga tcgatgaagt gaatgagcaa gctgggatca aaccagtaaa agtttctgat
     6601 gacgtctaca cgttagtcaa gcgggcctac gaatatagcc aagattctca aggcggattc
     6661 gatatggcga ttggtgcaat cacacaatta tggcgaatcg gttttgatga cgccagaaaa
     6721 ccttcccaag aagaaatcga tcaggcattg aaattagtgg attatcataa gatcgaacta
     6781 aatgacaaag aaaaaactgt ttatctaaaa gaaaaaggaa tgatcatcga tttaggtgca
     6841 atcgccaaag gttacattac ggacgaagta gtgaaagtct tgaaaaaaca aggagtgacc
     6901 actgccatcg ttgacttggg cggtaatgtc tatgtcttag gccacagccc aagaggcgaa
     6961 aaccaagact ggacagtcgg catccaagat ccgaacatgg ctcgcggatc tgttttaggt
     7021 tctattaaag aacggaacaa aacattagtt acctctggaa tttacgagcg ttatttagaa
     7081 gtagatggca aaaaatacca tcatttgttt gatccaaaaa cgggttatcc gttcgacaat
     7141 gatatcgcaa gtgtcacgat cataacagat aaatcaattg atggtgacgg gttgtctaca
     7201 gcagtctttt caatgggtgt gaaaaaagga ctggaatacg tcgaaagcga attgaataac
     7261 gggacggaag cgatcttcgt gacaaaagac gataaagtat atgtgaccga tccaatcaaa
     7321 gacaccttca aattatcgga ggactcccat tacacgatgg gtgaccgttc tgaactaaaa
     7381 taaggagtac gaaccatgac actaaaaatc tttttagaag tagttgaaat acgaacaaag
     7441 gtggctagtg tatttccttt tgcggtaggg gtattattct ctatcgcttt ttttcatgag
     7501 atacattggc taaacacttt tttatttttt attggaatgc tagtttttga tttagcgact
     7561 accgctatca ataattacat ggattatgaa aaggctcatt ctgaagtata taagtatgaa
     7621 gaaaatgtca ttggccgcag aaatctatct cctgtattga ttcgaaacat gattttagct
     7681 atgattgcat ttgtttttgt tatcggcgtt attctaacga tcaatacggg ttggctttta
     7741 cttttgatgg gatttatctg ttgttttatt ggagtattct atacattcgg accgattcct
     7801 ttatctcgaa tgcctttagg ggaaattttc agtggggtga ctatgggcct tggaattttt
     7861 gccatggtcg tttatgtcaa tacattcggt tcagatgtct atgatctgac agtcaacttt
     7921 tcatctggac aatttgattt ggtaggaaat ctttgggcta tagcagcaat cgtattagct
     7981 tccttaccct tggtgtttac gattgccaat atcatgttgg ctaataattt gcgggatctt
     8041 gaccgtgata ttgaaaatca tcgctataca ctagtttttt atatcggacg tcctgcggga
     8101 acagtcttat tccagctatt gatgtatgct tgctatttcg tgattttaat ggggttgttg
     8161 agcggtatct atgaatggcc gatcttggtc accttcttga ccttgccaat tgtgaagaag
     8221 catttggatc aattcaagca aacgttaccc cagcctatca gtttcagcta ttccatcaaa
     8281 aatatggtct tattcaatgc cagttatgta ttggggctat tactaacaat cattatgcaa
     8341 gccatttaac agcatgaaga ggttgtgaaa aaactgccct gcatcaagta ataagaacag
     8401 ccaaataaaa atagctcctt atatttttcg tttggttggg cttattaccg cagatgcaag
     8461 cttttgaaca ccgtttatca ggaggttgtg aaaaagtcgc ttagtttcga gtattaagaa
     8521 aaattttgtg aaaatggctt tccacatttt tgcataaatt tggcttaata ccgagaaact
     8581 ggcttttgaa caccgtttat cagaggttgt aaaatcagtg ttttgtcctg agtattagag
     8641 aaacaaatgg aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgaaggaca
     8701 gctgattgaa cactatttgt tcggaaaaag tgactaacta tgacacaatt tttttcacag
     8761 tccctttttt cacttgattt gattcgtacc agcgaacttg acgagaactt tcttggtttg
     8821 gtaactatat ggtattcttt taacatcctc gtttgcttct tctaatttat ttatacaaag
     8881 tcacactttt ttaaacatct gttcttcgat acatttctat ttcttttaaa catcgatacc
     8941 aatattttga acttaagagt gcataccaaa tggattcatt tactcttcgt cagttacgaa
     9001 gataatcaaa atctccaact tccctactta tttcgttttg agaattatcg aagcaatctt
     9061 acataaataa ctaaaaaaca aaaaaccaga aaatcagtat actgattttc tggttttaca
     9121 tggtttgttc aataaaaagc tgttggaact gttaatatta aattatcgca tcgttacgaa
     9181 ttcttccgct cctgttggat ggatggctac tgtattgtca aagtcttctt tagttgcacc
     9241 catcttgata gccacggcaa aaccttgcag catctcatcg acaccaacac caatagcatg
     9301 taagccgacg attctttctt cttttccgac acagatcaat ttcatttcgc atttttggcg
     9361 ataatcattt aatgcaaagt acattggggt aaagcgtgaa cgataaactt tcaattcatt
     9421 ttcgccatat ttttccaatg cttcttcttc tgtcaaacca attgttgcaa ttggtggatg
     9481 cgtaaagacg actgtcggaa caagtttata gtcaagataa gaatctttct ttccattaaa
     9541 taaacgttct gataaacgtc taccagctgc gattgcaaca ggagtcagat ctaatttacc
     9601 gatcacatca ccaacagcaa aaatatgttc atccgttgta ttttggtatt tatctacttt
     9661 gataaaccct tttttgtcca attccacatt tgtattttcc agacctaatt tttcggtatt
     9721 cggtacacgt ccaccggcaa aaagtacaca atcaccagtt aaggtttcac cattttcaaa
     9781 tagcaccgta tattcgtcat tgtttttagt aatttcttta gcaatatgat gtgcgtaagt
     9841 ttttattcca gcttcttggt acatttggat caaattgtcc gaaagcattt tatcaaacgt
     9901 tctaagtggt cgttcttttc gatatgccca catgacttca ctacctaaac cacgaaatac
     9961 acctgaaagt tcagccgcaa tgtatccagc acctaaaacg attgctctct taggtaactc
    10021 tgttaattca aaaaatccat ttgaatcaat tgcgtattct ccaccaggaa tggtcatttt
    10081 acttgcacgg cctccagtag cgatcaagat tttaggagca cgataggttt caccgtttac
    10141 ttcgatcgtt tgactatcga taaaagtagc gtaacctttt atagcctcga catgattgct
    10201 atctaatcct ctttgataag caccgtgtag aaaatcaata tatttttctc gtctttctac
    10261 tagaccggcg aaatcaacat tttttaaatc tgcttggata ccgtaacttg aggcatctcg
    10321 ttcaattgtt tccaaaatcg tacttgcttg ccacataact tttttaggta cacaaccgac
    10381 gttgacacat gttccgccca attcattcgc ttcaatcagc aaaacttttg cgccatgcat
    10441 ccctgctctg tttgctgaag caataccgcc agagccgcca ccaattacga tgtaatcata
    10501 tatttgcatt ttgttcctcc ttattgaaga gatcaatcaa aacctttatt taacgatcct
    10561 ttttctacag aaaacaagtg taacactttt cttgaatttt gtatgcgaag atgcttaaaa
    10621 cagggatata tcagcttttt ccttatttac gttttcggat catatctcca ggtttcaccg
    10681 gctgtgcgac tggcatagtt aaaatcatca ttggatttgg tgctcggtca atggattcgc
    10741 cttcttcatt atgcataact tcaacggttt gactgaaatg actgaatcct ggaccataaa
    10801 attcgatttc atctcctaca gaaaacaagt ttctctgacg tatcgtagca atctgtgttg
    10861 cctcatcata agataatact tcaccgacaa atttatatac tgggatttta cgacgttcac
    10921 cgaataattg ttcgttttca gttggtgtat tgtagtaaaa acctgtagct aattctcgtt
    10981 gtgctacttt ccataactcg tcgatccatt cttgcttgca gacatagttt tcaggatctt
    11041 ccatataact gtctacagcc gctttataga catttgacac cgtagataca taatggatcg
    11101 atttcattct tccttcgatc ttgaagctgt ctacgccgtt ttcaatcaat tctggaatat
    11161 gttcgatcat tgacatatcc actgcactca tcgagaattc ttcttccact tcgcctgtat
    11221 cagttaaact gtttcgttct tgaccaaaag gcatatcgaa caatccatac ttccagcgac
    11281 aagattgaga acatccgcca cggtttgcat cccgcatcga catatggttg gacaatgtgc
    11341 atcgaccaga ataagagata cacatggcac catggataaa tgcttcgatt tcggtatccg
    11401 tatttttacg aatctctgcc acttcctcca ttgaaacttc acgagctaaa acgacacgtt
    11461 ccaacccttc ttctttccag aattccaatg tctcaaaatt agttgctgaa gcttgagtgg
    11521 ataaatggat cggcagacct ggggcttctg tcgcacaaat ttcgatcaaa gctggatcag
    11581 aaacaatcac tgcagaaata ccgacatcac gcaaactgcg gaaaaattcc cctgctccat
    11641 cttcatttcc ttcatgggtg accatattag cagcaacgta taccttcgca tcatgttctt
    11701 ttgcaaaagc aactccttca gccatttctt cataagtaaa attacctgca cggcttctta
    11761 aaccatatgc attcccgcca atatatactg catctgctcc ataatggatc gcggttttta
    11821 acttttccag tgttcctgct ggcgctagga tttctggtcg tttcaatttt ctttttactg
    11881 tcatttcttt catcatactc cttatttgat agcagaagga tcaatataaa agaatcctgt
    11941 atctaattca cggttttcag gatgcaattt actgatttcg ctagtcattt tttccgcttt
    12001 ttcttgatcc cagctacctt ctaaaatttg tttttttgct tcggcgaata gtcgagtgat
    12061 gtttacgaat ttttcgcctg gagtaaataa tccgtccaat ttccatgtac agtaatgatg
    12121 ctgataaagt tcattcaatt cattcgagag attcagatca ttgctggcaa aaatatgtgt
    12181 gccatgacta tcttcataaa tagaataatg tgtctcttct tttttaggtt ccgagataaa
    12241 aagacctctt tcacgatctt tttgttcgtc ttgtttcgta tagttatagt aattctgtag
    12301 cagtggacgt ttggattgat ggatgcatgt agcaccatac acaagggttt ctacaggaat
    12361 atcgagtttt tcttccatcg ccttcatctc ttcaaaagga acttcacgag caagtactgc
    12421 tccaactgcg ccttttttcg cccagaaatt gatttgacgt gcgctcgtta ctaatgtttc
    12481 agcatcataa atatacggta tacgcaattc aggatttttt ttcatcgtat aaataatacc
    12541 tgtatcacca actgtgatca catctacgtt tatttctttt aaaaaagcca aatattcagg
    12601 tacatttttc attttttcag gatgaatgat cccatttaca gcaatattcg cttttttacc
    12661 atacttgtgt gctaattcta ccaattgtcg ctgttcttct cgtgtaaatg aagcaggcag
    12721 acgcaatcca aattgctctt caccgaaata tagtgtatct gcacctgcag caagcaactg
    12781 ttctgcttgt tcaatagttt ctactgtggt tatgagttcg atcataattt tctcctccta
    12841 caacttcata atagtagcat agctctaaaa actttccaat acttcttcta tttaaagtcg
    12901 gatttttgaa aattatttca caaaaaatca aaataaagtt cgatatattc tgtccaaata
    12961 taaaaagaga ttgtgacaaa aatcttgtca caacctctta atcaagtctt actattataa
    13021 aaattcagct acacaggcca agtaactata gggaacctac tgtttccttt ttaggtccgc
    13081 gaacattcaa tgtgatctta ccttttgaag cacctactgt tacgtggtca cctaaacgaa
    13141 tttctccgcc aagcaaagcc tcgctcaatc gatcttcaat ttctttttga agtgcgcgac
    13201 ggattggacg tgcaccatat tcaggatcaa atccagcttt cccgatcaca tcgattgcag
    13261 atggtgtgat tttcacttga atatcttgtt ctttcaatcg tttgataatc gctttgctca
    13321 tgatttggac gattgtatgg atttcatctt tactcaatga atggaagacg acagtctcat
    13381 cgattcgatt caagaattca ggtcggaaag ctttctttaa ctcttctaag atacgttttt
    13441 gcatggcttt gtgatcttta gtaacatctt ggacattgaa tccgacattt ttttcttctc
    13501 gaatttgtgt cgctccgata tttgaagtca taatcatgat tgtatttcta aagtccactt
    13561 ttctaccttt agaatctgtt aaatgaccat catccaatac ttgcagaagt aaattgaaaa
    13621 cgtctggatg tgctttctcc acttcatcta agagaatcac tgaatatggt ttttgacgaa
    13681 ttttttctgt caactgtcca ccttcatcat accctacgta tcctggagga gacccgatta
    13741 atcggcttgt actgtatttt tccataaatt ctgacatatc aacacggatc agggcatctt
    13801 cacttccaaa catgacttct gaaagtgctt tggccaattc tgtttttccg acaccagttg
    13861 gtccaaggaa catgaatgaa ccaattggac gatcaggatc tttcaatccg cttcgtgcac
    13921 ggcggatcgc acgtgaaact gctttgacag cctcatcttg accaacgaca cgttcatgca
    13981 gaagtccttc cagttctaac aagcgttcgc tttctttttt ctccaactgc tgtagaggga
    14041 caccagtcca ctccgataca actgtagcaa catcttcttc tgttacacga tcagaataac
    14101 cttgctcctg ttttgcttca attgcagaaa ctttttgcag ctttttacgt agtgcttttt
    14161 cttgtctgcg taattgagca gcattttcaa agtcttgttt ttggatcgct gcttcctttt
    14221 cttgtactag ttcatcgatt tctagtttga tcacagttga ttttgttaag tgatcggttt
    14281 gatccaaacg gactttcgct gctgattcat cgatcaaatc aattgcttta tcgggtagtt
    14341 gacgagaagt gatataacga acagaaagat tgactgctgc tcgtacagct tcatctgtga
    14401 tctccactcc atggtgctct tcatatcttg tacgtaatcc ttgaagaatc acttctgctt
    14461 cttcaggagt tggctcatct acttgaatgc gagcaaaacg tcgctctaat gcagaatctt
    14521 tttcgatata tttttgatat tcatctaggg tcgttgctcc gatcgtttgt aattctcctc
    14581 gagctaacgc cggtttcaag atattcgaag catcaattgc tccttctgct ccacctgcac
    14641 caattaaggt atgcaattca tcaatgaata agattacttg accatcatta tagatttcgt
    14701 cgatgacctt tttcattcga tcttcaaatt caccgcgata tttcgtacca gcaaccaaag
    14761 atcccatatc aagcatcatc aggcgctttt cttgcatgtc ttctggcact tcacctaaaa
    14821 tgattttttg agctaaccct tcagcaatgg ctgttttgcc tacaccaggt tcacccacca
    14881 gtacagggtt attctttgtc cgtcggctaa gaatctggat aagccgtttc acttctctcg
    14941 aacgaccaac aactgggtcc agcttgtttt cttttgctaa tttcgtcaaa tcacgtgcta
    15001 atgaatctag cgtaggtgtt ccttgcggtg tttgccgttt atcttgtaca tttacatttc
    15061 tacgacgatt cattcctttt ccagcagaat caccagctag ccccattttt tgtttcagaa
    15121 cttgacgggc ttttgaaagg ctgataccta agttgatcat gattcgagta gccatgatac
    15181 tttcatcttt taataagctc atcaaaatat gttctgttcc aactttttga gaaccagttt
    15241 tcttcacttc gctgctagct aaagcaaaaa cttgtttcat tcttggtgaa tatggaagaa
    15301 agactccttt agggtaagaa cggataccac cataaccaat cagatgttca atttcttcat
    15361 aaatttccgt ttcattcaaa tccatttcac gtaatacttt tccagcaatt ccattttgct
    15421 cgaccactaa ggctagtaaa acatgttctg aactaactga ttgatgtttc aaccgttttg
    15481 cttcttcttg tgcaatttct aatactgctt gggctctttc agtaaaaagt tcttccatct
    15541 aatctcactt cccatcttca taacttaaac gttccagaaa tgctcggaga atccgagcac
    15601 gtgtatgatc ttcatattca ttgaaattca atgtgttttt tgcaattgca ctcaacatca
    15661 agtttccttc tttttttgta attatttggt cttcataaag tttttgaata atagcaaaag
    15721 catttttctc actgattgta tcatcaaata actgatttat ctgctcaagt aattgttttt
    15781 tatctgaaat ccttactttt gcaattcgta tataaccacc accaccacgt ttgctttcta
    15841 ctagataacc acgttggatg gtaaaacggg tattaatcac atagttgatt tgtgaaggaa
    15901 cacagttaaa gaggtctgcc atttcaatcc ttcgaatctc gatcatttca ctattttcca
    15961 gaattttctt tatatatgat tcaattaaat ccgaggtatt ctgatgtgcc atcttattca
    16021 ttccctttga ctaatattga ccttaattat aacgaatttg ataatagaaa gaaagaagaa
    16081 cgctttttgt taaataaatc ctttagaatc aacaaaaaaa aatacggaat atactattcc
    16141 gtatc
//
LOCUS       DACECY010000059        15869 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.063, whole genome shotgun sequence.
ACCESSION   DACECY010000059 DACECY010000000
VERSION     DACECY010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.063"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            415..978
                     /gene="efp"
                     /locus_tag="IWO38_002095"
     CDS             415..978
                     /gene="efp"
                     /locus_tag="IWO38_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ0314174.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            1464..2723
                     /gene="lysA"
                     /locus_tag="IWO38_002096"
     CDS             1464..2723
                     /gene="lysA"
                     /locus_tag="IWO38_002096"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ0314175.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            2710..3510
                     /gene="dapF"
                     /locus_tag="IWO38_002097"
     CDS             2710..3510
                     /gene="dapF"
                     /locus_tag="IWO38_002097"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ0314176.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            3513..4394
                     /locus_tag="IWO38_002098"
     CDS             3513..4394
                     /locus_tag="IWO38_002098"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ0314177.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            4391..5107
                     /locus_tag="IWO38_002099"
     CDS             4391..5107
                     /locus_tag="IWO38_002099"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ0314178.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            5113..5808
                     /gene="dapD"
                     /locus_tag="IWO38_002100"
     CDS             5113..5808
                     /gene="dapD"
                     /locus_tag="IWO38_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ0314179.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            5824..6825
                     /locus_tag="IWO38_002101"
     CDS             5824..6825
                     /locus_tag="IWO38_002101"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ0314180.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            6822..8183
                     /locus_tag="IWO38_002102"
     CDS             6822..8183
                     /locus_tag="IWO38_002102"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ0314181.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            8143..9294
                     /locus_tag="IWO38_002103"
     CDS             8143..9294
                     /locus_tag="IWO38_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ0314182.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            9269..10438
                     /locus_tag="IWO38_002104"
     CDS             9269..10438
                     /locus_tag="IWO38_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0314183.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            10613..11992
                     /locus_tag="IWO38_002105"
     CDS             10613..11992
                     /locus_tag="IWO38_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0314184.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            12273..13070
                     /locus_tag="IWO38_002106"
                     /pseudo
     CDS             12273..13070
                     /locus_tag="IWO38_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            13259..13927
                     /locus_tag="IWO38_002107"
     CDS             13259..13927
                     /locus_tag="IWO38_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ0314185.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(14127..14513)
                     /locus_tag="IWO38_002108"
     CDS             complement(14127..14513)
                     /locus_tag="IWO38_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314186.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(14485..15867)
                     /locus_tag="IWO38_002109"
     CDS             complement(14485..15867)
                     /locus_tag="IWO38_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ0314187.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
BASE COUNT         5228 a         2805 c         3399 g         4437 t
ORIGIN      
        1 aaaaagataa taaaaaaagg agctgactat taaatcagct ccttgaaaga catagagaaa
       61 aattgagtag ataaaaagat aagttacctg gtactcattc tatgaagggc aattaataga
      121 catgacaaat agtatgctgc ttttatgcac catacaacag caacgattca attgtttttg
      181 gttgctgact gtcatgtgta tccctccaag taaaattttt attaacgata gcatgaaaaa
      241 aaataaaagt caaaggcttg aaagaaaaaa atatttcgat agcatcgttt acccgtgttt
      301 atttttctga caaataggca tgagacctta aaagatagag aatactctag ttttctggcc
      361 ggttgtctga tacaataaag ttgattatga taaagaaaag aaggagcaaa aataatgatc
      421 tcagcaagtg atttaagagc cggtatgact ttcgtacaag acggcaaact gattaaagtt
      481 ctggatgcaa gccaccataa acctggtaaa ggaaatacag ttatgcgtat gaaactgaaa
      541 gatgtccgtt caggtgcaac atacgacaca acgttcagac cagaagaaaa atttgaaaaa
      601 gcacatatcg acacaaaacc tgtacaatat ttgtacacga tggatgatac agctttcttc
      661 atggatttgg aaacatatga gcaatatgaa attcctgtag aaacagtaga agaagaaatg
      721 aaatatatcc tagaaaacat ggaagtaaaa atccaattct tcggtagcga agtcatcggc
      781 gttcaattac caacaactgt cgtattacgt gttgtggaaa cacaaccttc catcaaaggt
      841 gcaacagtca ctggttcagg taaaccagcg acaatggaaa ctggcttagt agtcaatgtt
      901 cctgacttca tcgaaacaga tgaattgttg gaaatcaata cagcagaagg ttcttatgta
      961 aaacgtgcgt caaaataatt cgctaacgga ttgataagaa tttgaaaaca gagcttgacc
     1021 tcttgaaagg cagatcatcg gatctgcctt tcaagaggtt ttttgtggaa tcagcaaatt
     1081 gaaaaataaa aaagtatttg attttattta tattttgaaa gatgaatggc aaaagctaga
     1141 gaaagaaaaa actgcactta tttatgaaga aattgaaatg attcataaaa ttgaaaatat
     1201 ccgaactatt ccgaactata ttgaagcgaa aatatttttt catcactaaa tagtgttgaa
     1261 tttatgtttt tatgtataaa tagcttctaa tagttcgggt attttgtttt tataaccatt
     1321 tttattctta tttttaaaaa aatatttcga aatatactag acaattcaga aaattttgtt
     1381 taatattatt ccaataaaat tcaaatcttt atcaaatgaa attccaataa aaacggaaaa
     1441 aaagtgaaaa gaggaatgaa aaaatgacag aattgataat tggcggagtc acagcaagca
     1501 cattggcaga aaagtataag acacctctgt atgtgtatga tgaagaaaag atgagacaaa
     1561 cgatgctgtc attcaagcga ggctttgtat cgaaacgttt tgatacaaaa gtgctttacg
     1621 cctctaaagc attccaaacg attgagatgc tgcatttagt tcgtgaatat ggattcgggc
     1681 tagatgttgt cagtggtggg gaaatctata ccgctttgca agctgatttt cctagagata
     1741 ctatctactt ccatggtaac aacaagacgc cagaggaact gatctatgca cttgaaaatg
     1801 atttgttgca tattgttgcc gataatttga tggaagttga attattagca gaattaagcc
     1861 aacgctattt aaaaaaaatc tgtatcatgc ttcggttgaa tgtcggagta gaagcacata
     1921 ctcatgaata tatcgtcact gcgcatattg attcaaaatt cggcatgagc tatgaaagta
     1981 aagagtgcca acaagccctt cagttgataa aagaaagcag ctatctagag ttagaaggat
     2041 ttcatgctca tatcgggtca caaatttttg atgttactgc ttggttagct gaaatcgaca
     2101 aattgacgaa ttacttaacc gattttgacc aacctctttc cttgaatctt ggcggagggt
     2161 ttgggattcg ttatacacaa aatgatcagc cgatcccagt cgaagaggca ctaaaaaaac
     2221 tgattgaata tacggaagaa gctttggaaa aacgaaaact gaccattcgt caattactga
     2281 tcgaaccggg tagaagtatt gttggagaag ctggtacaac actatacacg attggtttca
     2341 tcaaaaagac accacataaa aaatattact ttgtagatgg cgggatgaca gataatatcc
     2401 gtccagctct gtatcaggca gaatacgatt gtgatttagc agcaaaactg tcggaagaaa
     2461 aaacagagaa agtgacgatt gcaggaaaaa tgtgtgaatc aggcgatgtg gtaattaaag
     2521 aagcttattt gccgaaagca gaatcgggtg atcttttagc tgtttattcc acgggagctt
     2581 atggatactc gatgagcagc aactacaatc gagcgacacg accagcagtt gtttttgtca
     2641 ataatggcca atcaagactg attgttcgca gacaagcatt ttctgatcta ctaagggggg
     2701 aagtttccta tgatccttca taaatataat ggctgcggaa atgattttat cttattagat
     2761 tacgcagaag acacagatta tagtcaatta gccgcttctt tatgcacaaa agaaaaatat
     2821 gatatagacg gattgatcgc cgtaaaaatc gatccgttgg aaatgatcta ttacaacaaa
     2881 gatggcagcc gcgcgccgat gtgcggaaat ggcattcgct gttttgctcg atacgtaaat
     2941 gatcagaaca tgatgaatga acaggaattc aacgtagaga cattagcagg cacaagaaaa
     3001 attacggtgt tggatccctc acctttttat tgcagcgtag atatgggagt gccagatgat
     3061 tctccggaaa aaatcaaagc taatcaaact tatccaataa aaaaccagga aatagagatc
     3121 aatggtgaaa aagtaacgat cactagttta tttttaggaa cgattcatac cgttgttttt
     3181 gttgaaaatg cgcgtgagga agtctggaga aaaagaggtg aactgatctg tcatcatcct
     3241 ttgttcaaag aaaaaaccaa tgtcaacttt gtagaagtag tcaatgaaca agaattgatc
     3301 gtccgtacct atgaaagagg agtaggttgg acacttgctt gcggtacagg ttgctgtgcc
     3361 tcttatgtgg ttgctaagga aactgggaaa atttcagcag atcaggtagt tgttcacttg
     3421 gaacaaggag acttgatgat ctctggtgag gatgcgatac aaatggctgg cccagcagtt
     3481 catgaatgga caagaatatt ggaggaatga agatgataaa aggttcgatc gtggcgctga
     3541 tcacgccgat gaatgaagag ggatcagtag attatgctgg tttggaaaaa ttgatccaat
     3601 tccatttaga tgaacaaaca gatggattat tagttttagg tacaacagga gaatcttcta
     3661 cgctcacaca atctgaggag gagcagatcc ttcaactgac agtaaagaaa gtggctggcc
     3721 gtgtgccggt gattgcagga gctggaacga ataatacgaa agaaacgatt gaaaaagcga
     3781 agcattttgc ttctttgggg gcagacgcac ttttggtcat caccccttac tacaacaaaa
     3841 caagtgatgc aggactagct gctcatttta cagcaattgc agaagcctca ccgattccgc
     3901 ttattttgta taatgttcct tctagaacag gcatgtcgat tccaatccat gtattagtaa
     3961 atttagcaga acatcctaat atcattggtt tgaaagaagc ctcaggagat atggcttatg
     4021 tgatggatgc cgctcggttg attggagaag aattcttcct ttacagtgga aatgatgatt
     4081 tgatcctacc agtaatgagt gtaggaggaa gcggtgtaat aagtgtatgg gcgaatatcc
     4141 aacctaaaat agtccatgaa ttagtaaaag atacgcagga cggaagatgg cagcaagcaa
     4201 aagaaaagca attaaatgca cttgaactga tccatgcctt gttcagtgag accaatccta
     4261 ttcctgtcaa agcagccatg tcattattag acttaccttc cggacctcta cgcctgcctt
     4321 tagtttctct ttcaaaagag aaaaagaaac aattagctca gcttttgtta aaagaaagga
     4381 cggttgaaaa atgaaaatcg gactgattgg cagtggacag atgggaaata gagtcgaaga
     4441 agttgcacaa gaaagaggag atacggtcct tcttttacgg acggcaccta aagaagcaac
     4501 tacgcttgct tttacggaac tgccagaact gcttattgat ttttctcatc cagataattt
     4561 agagaaaatc ctttcgtata gcttatcata tcaacttccg ttagtcattg gcacgacagg
     4621 ttatacacaa tcacagttcc aagaaataaa agaacatgcc aaacatgtac ctgtgatgta
     4681 tagtgctaat ttttcgttcg gcatcatggt gatgaatcaa ctaatcaagc aagcagctgc
     4741 aatgttgcaa ggctggcaga tcgaactgat tgaaaaacat catagtcgta aaaaagatgc
     4801 accatctggt actgcaaata tgctgcttca aacaatccaa gaagtgcaaa agctgcagcc
     4861 cgtatacaac tggcaaggga aaaaaagaga agaaaaccaa attggtgtac acagtattcg
     4921 tgcaggttct ttacctggtg aacatgatgt attgtttgct gctactgatg aaatatttac
     4981 gatcaaacat gaatcttttt ctaatcgaat ctttgctgca ggcgcagtag aagcagcgga
     5041 ctggttgaaa gatcagtcac caggttatta caaattagaa gatttattaa tggacaaagg
     5101 ggtgtaaaaa acgtggaagc tcaagaaatc atccgctaca ttcaaacggc atctaaaaag
     5161 acgcctgtga aagtctatct gaatgtaacc gagccaatcg aatttaaaga aagtaaagtt
     5221 tttggtgaag gaagcagctt cgttgttttt ggtgactatg agacgattgc tccggtttta
     5281 gaagctgaat cagaaaaaat cattgaaatc gaaatcgaaa ctgctgcaag atacagcgca
     5341 gttcctttgc tagatattaa aaaaattaat gctcgtatcg aaccaggtgc tattatcaga
     5401 gatcaagtgt caatcggcaa caatgccgtg atcatgatgg gagcgattat caatattggt
     5461 gcagtaatcg gtgaaaacac gatgattgat atgggtgctg tcttaggtgg acgagcgaca
     5521 gtgggcaaaa actgccatat tggtgcagga gctgtcttag caggtgtgat cgaaccagca
     5581 tcggccaaac cggttatcgt agaagacggc gtactggtag gtgcgaatgc agtgattgtt
     5641 gaaggtgtcc atatcggaaa agatgcagtc gtggcagcag gagcagtggt tttagaagat
     5701 gtggcagcag aaactgttgt cggtggaata cctgcacgtg tattaaaaat aagtgatgat
     5761 cagacaaaag aaaatacagc attgattgct gcgttacgcg aattatagag gagatggaga
     5821 aaaatgcgcg aaatagatac ctgtattgtg ggggcaaccg gattagtcgg ccaaacgatg
     5881 ctcaaagtgt tagaagaata tgattttcca gtaggacgat tgaaattatt agcttcaaaa
     5941 cgttcagccg gtaaagtttg tgtattcaag aaaaagaaac acactgtaca ggaattgaca
     6001 gaaacttcat ttgaaggata tgatttagcg ctattttctg caggtgcttc tgtatccaaa
     6061 gagtttgcgc caatcgcacg aagtcaggga ctatttgtca tagataattc ctcagcatgg
     6121 cgggaagatt cggatattcc attaatcgtt ccagaggtca atctggaaga ctatacattg
     6181 aatcggctga ttgcaaatcc aaattgttca accattcaag cggtacttcc gcttaaagct
     6241 ttacaagaag cctttggtat ccagcgagtc aattattcga cctatcaagc agtttctggt
     6301 tccgggcaaa aaggcattga agatcttcat cgtacgcaaa atgacgagga accagttttg
     6361 tatccttata atattagcca aacggtgatt ccggaaatcg atcagccttt ggaaaatggg
     6421 tatacaaaag aagaacagaa aatgataaat gagacccgta agattctgca tcagcctgct
     6481 ttgccagtat cagcaacctg cgtacgtgtg ccaatcgaaa atggacatgg agtctctgtg
     6541 gcagttcaac tgcaaaaaga gtttacgacg gaagaaatta gagagtgtct cagccagttt
     6601 ccagggattc aattagaaga tgatctagca gcacataaat atccgacgag cattcttgct
     6661 agaggaacag atatggttta tgttgggagg atcagaaaag acacttcttt agagaacgga
     6721 ttgttgttct atacgactgc cgataacata cgcaaaggag ccgctgcgaa tgccgtacag
     6781 attgctgcag ctttaatgca gaaaatagcg gaggtggcaa gatgagtaat acgctgaaag
     6841 tagcgaaatt cggcggaaca tcgatgtctc atgaagagca attcaaaaaa gtccgtacaa
     6901 ttgttctttc agatcccaat cgccaagtga tcgttgtttc tgctttaggc aaacgtacgt
     6961 cagatgatga caaagtaaca gacttacttt atttgattta tgcttacctt cagcatcatg
     7021 ttgattggac acccctttgg aaaaggattt gtgatcgttt cattcaagta agagatgttc
     7081 ttgccctgca gttacctatt gaagatgaac tagcacgcat acagaaacaa ctggaagacg
     7141 aaaagataac tgaagattat ttagtaagta gaggtgaata tctgactgct caactgatgg
     7201 ctgagtatct tggttaccag tttattgacg caaaagacct gattttcttt gactatgatg
     7261 ggaaaatcga tcaggaaaaa acagcacagg aaatcacaaa agcatttaaa agatatccga
     7321 aaatggttgt tccagggttt tacggggcga accctgcagg aaaagaaaaa ttattgggcc
     7381 gtgggggaag cgacattacc ggcgcgatac tggcgaagat gttgcaagcg gaaagttatg
     7441 agaattggac ggatgtttca ggaatcatga tggctgatcc gcgaatcatc gatcatccta
     7501 aaatcatcga ggaaatctct tttagagaac tgagagagat ggcttatatg ggggctaacg
     7561 ttcttcatga agcagctgtt ttcccagttc aagaggctga tatccccatc caaataaaaa
     7621 ataccaatga tcctttcgct caagggacaa aaatatcaaa tcacgaaata gaaaaggaaa
     7681 atggactgac aggaatagct ggacgcaagg attttctttc catcacttta ttcaaacgtc
     7741 atatgtccga tgaaatcggg tttatctgga aagcaatgag catttttgct aaacatgaga
     7801 tatcaatcga acatattcct tcagggatcg acaatatcgg agtgatcgtc tcagcagaag
     7861 cgattgccga taagttattt ctcatcacga aagaattaaa agaaacactt ggcgtggaag
     7921 aaatcgtcgt gatcgaagac ttagctttga tctctgtcgt tgggggtccg cataaagaat
     7981 tgattggatt atctgggaaa gtgttatcga tattgaacaa attagaaatt cgcacatcga
     8041 tcttatcaca aggagcacag gagttgaatc tgatcatcgg tgtaccgaat aatcaatatg
     8101 aaacagtagt gaagggtatc tatgaaggga tggtgaatac ggatgctgaa agaatatcga
     8161 atggcactcc atcaaattcc tgaaatcggg tttcatgaat tcaagacaaa aaaatattta
     8221 tatgaacaag taaaaaacag cgaggccgtt atccatgaaa tcggcgaaac aggactactc
     8281 ctttattttg ataatcagca agaagaaacg attgcttttc gaacagatat cgatgcactg
     8341 ccagtcaaag aagaaacgaa tctctctttt gcttctaagc atgaaggtta tatgcatgca
     8401 tgtggtcatg acggacatat ggcgatgctt ttagggttga ctgactttat aactaaacac
     8461 attgaggatc ttatttataa tgtagtactg atctatcagc catcagaaga aatttccggt
     8521 ggcgcacaaa gcgttattca gtcaggattg ttggataaat atgaagtaca agcaatattt
     8581 ggctttcatc tatggcctgg actagctaag ggagaagttt tctctcgacc aggacctttg
     8641 atggcacaaa gcagtgaaac agacattatc attaaaggcc agtctgcaca tattgcttct
     8701 agtgaaaaag gaatcgatag tctggaggca gctgtacgat ttttgaagaa tgtttatgat
     8761 tttgaccggc aattaccggc agagtggaat catctaatga aatttggcca aatccaaggt
     8821 ggaacgatcc gaaatgtttt agctggagaa gttattgtat ccggaagtat ccgttcttac
     8881 agttcagata tgcaaaatta tttgaagagg ggattagacc atttagcaga acagtttcac
     8941 tctacagcta ctatttcttt tcgctataat gacggctatc cagctgtaat caaccataga
     9001 ggcttatatg cagcactgtt agattcaaac aaactacatg aattagatgc tccagttctt
     9061 caagccgaag attttggcat atatacgcaa caatatccat gtgtattttt ctttttaggt
     9121 gtcggtgata cccctgcttt acatgatccg acgttcgatt ttgatatgga tgttttagaa
     9181 aatggtgtag aatggtacaa aacgattctt tttacagaaa atcttcttgt aaaaatcgat
     9241 agcagttcaa aagaggagcg agtaaataat gatgaattta tccaatcgat ttagtcctag
     9301 gctttctaga atagaagtat caaaaatacg acaattcgat cagcaaatat cgtcgatacc
     9361 agatgtgatc aaactcaccc tgggagaacc ggattttcca actcctgaac atgtcaagca
     9421 agcaggaata gcagcgattg aagaagactt ttctcactat accggaatga gaggattaga
     9481 ggaactgcgg gaagcggctt gtatatttca gcaacagcgt tatggattaa cttatgatcc
     9541 gcaaacggaa gttttgacga cagttggtgc aacggaagcg attgcctctg cgttgttatc
     9601 tgttttggaa gaaggagaca aggttcttat tccagcgcct gcttattcag gatatcagcc
     9661 attagtggaa ctagctggtg ctgaattgat cccgatagat acttctgaca cagggtttgt
     9721 ctgtcagccg gaacaatttg aacgagcatt tgaacaatat ggatcagctg tcaaagcagt
     9781 cattttaaac tatcctaaca atcctactgg gacgacattg tctgcaaaac aattgaaagc
     9841 aatcgctgag gttttaaaaa aatatccggt atttgttata agtgatgaag tatatgctga
     9901 attgacttat agtggaacgc atatgtccat agctacttat cttccagaac aaacaatcgt
     9961 gataagcggg ttatccaagt cacatgcgat gactggttgg cgagtgggct ttatttttgc
    10021 tcaaaaagcc cttattgacg aactaattaa agtccatcaa tatctggtga ctgccgctac
    10081 aacgatgtcg caaaaagctg cggctgaagc gctaataaat ggggtgaaag atagcgagaa
    10141 tatgaaagag caatatcaaa cacgaagaga ctatctggtc cagcaattgg cgcctttagg
    10201 atttgaagtg acccagccaa acggtgcatt ttacttgttt tgcaaactcc cagctgccat
    10261 tcaaactgac tcttggcagt tttgtgtaga attagcggaa caggcaaaag ttgcgtgtat
    10321 cccaggaatt gcatttggcc cagaaggaga aggttatatc cggatttctt atgctagcag
    10381 catggaaaaa ttacatgagg cctgcaaacg gatcaaagac tttttaacag aacagtgatt
    10441 catagatttt atcaaaaaaa caatcgttaa ttttgcggta tattctgcaa ggagacgatt
    10501 gttttttaaa tttagttcaa atgaacgcgt ttcttttgat tcataactaa tgtaatgtta
    10561 agtttaaatt gtttagaatg attttaaaag aatattcaag gaggtttcat caatggaaaa
    10621 aatcaaagtt gtcattgtag gtgcatctca tggagggcat caatcgatct tggaactatt
    10681 gacacgatac ggtgaaaatg tagatatcac gctatttgaa gcaggagact atatttcctt
    10741 catgtcatgt ggtatggagc tgtatttaga agacaaggta acagatgtaa atgatgtacg
    10801 taatttcaga ccagaaaact ttccgcaatc caatgttcat attttaaata atcacgaagt
    10861 aaaaacgatc gatgccgata aaaaaacagt aacggttact agcgcatcag ataacgaaac
    10921 aaaagaatat ccttatgaca aattgatttt gagttctggt gtcaaaccaa acaatctgcc
    10981 agtaccagga gcagatctag aaaatgtcta cttgatgcgt ggatacaatt gggcaaccaa
    11041 aatcaaagaa aaattaaatg atccggctat taaaaaaatc gctgtcgtag gtgcgggtta
    11101 cattgggatc gaagcagcag aagcaagccg aaaagctggg aaagaagtcg tattgcttga
    11161 tgtgatcgat agacctttag gcacttattt agatgctgag atgacagata ttttagaaca
    11221 acacctaaat gaaaacggta ttgaagtttt gacaaatgca aaaatcgaag cttttactgg
    11281 taacggaaaa gttgaggcaa tcaaaacaag tgaaaaagaa atcccagcag atctagtcat
    11341 tcaagctgcc ggtgtgaagg caaatacaga atggttaaaa gggatcgtcg acttagatga
    11401 acgtggctgg atccaaacaa atgaatactt gcaaacgaat cttccagatg tgtatgcagt
    11461 aggagatgct acacttgctt attctatccc agcacgcaaa aaaatgccga tcgctttagc
    11521 gactgttgca cgtagagaag cacgttatgt agtaaaacat ctttttgaag aagtaccaaa
    11581 caaaccgttc ggcggagtcg taggttcctc tgcattaagt gtctttgact atcattttgc
    11641 agcaagcgga ttgaatagct ttacagcagc aaaagcagga gtagatgtac gagtgtctta
    11701 ttatgaagat acgttacgac caaaatatgt accagaagaa caaggaaatc caaaagtgtc
    11761 catccaatta ttctcggatc caatgaccca tcaattgcta ggaggagcag tattatctac
    11821 ctatgatgtt acaggtcaag gaaatgtatt ggcactagcg atccaaaaca aactgacttt
    11881 agaagaacta gcagaagctg acttcttctt ccagccaggc tttgaccgac aatggagcat
    11941 gttgaatcta gccgctcaac aagcgttagg agaagaacca tttgtagaat aagaaaacga
    12001 aatatttgaa aagaggttgt gaaaaagctg ccttgcatca agtaataaga acagccaagt
    12061 aaaaacagct tcttatgttt ttaacttggc tggacttatt accgcagatg caagcttttg
    12121 aacaccgttt attttcaaaa aagggactgt gacacgattt gtgtaccgac ccccaaaagt
    12181 tagaccaaaa aatctaactt ttggaggtcg gtatttttta tggttaaata tacttttgaa
    12241 cttaaattaa agatcgttca tgattatttg gatggcaaag gcgattggac cgtaaaaatc
    12301 cagatcaaga gattgaagcg gaaatgctta aaattcggga gaagcacaag gattatggct
    12361 gtttacggat gaccaatgaa ctaagaaacc gcggtttctc tatcaataaa aagaaagttc
    12421 agcgcttgat caagaagcta ggaataaaag taacggcata cacgcgtaaa tcacgtcgct
    12481 acaattctta tcgaggaaaa gttggagcag tagccaaaaa tcggatccat cgtcgtttct
    12541 acaccagtat ctgccatcag aaaatcacga cggatacaac tgaatttaaa taccatgaag
    12601 tggatgccaa aggcattatc cgtcaaaaga aattgtatct agatcctttt atggatatgt
    12661 tcaatagtga gattttatcc tatcgtatct ctgaaaagcc caatgcatta gcggtaatgg
    12721 aaggtttgga ggaagctatc cagaccacta acgattgtcc ctatcgtcgt acgtttcact
    12781 cagatcaagg ttgggcgtat caaatgaaag cttatagaaa caaattgaag gagtacaaaa
    12841 tctttcaaag catgtctcgg aaagggaact gcttagataa ttcacttatg gagaattttt
    12901 ttggcttatt aaaacaagag atttttcacg ggaaagttta taactgtttc gaggaattaa
    12961 aatcagccat cgatagctat atctattact acaataatga acggataaaa cagaaattaa
    13021 actggcaaag tctcgtccaa tttcgtaaaa caaccgtaac ggttgtgtaa aaatatcatt
    13081 ttagagcata aaaaaaagga gtggatttct ccactccaaa aaagtctaac ttttgggggt
    13141 cactacagtg tcacagtcct ttttttagta aaaggataca tttcttcctt gtatattcca
    13201 tgcgtgttcg tgagaaagca tatataattt aattaacaga tagttagggg gagaaaaaat
    13261 gattttttca attacaatga aactggtgtt cgggttgatc ggtttattat tagtggtacg
    13321 tttgcttggg aagaaatcca tgtcagaaat cacaccgttt gatttagtct atacgcttgt
    13381 tttaggtggg atcttagaag aatcaaccta tgatgacaat gtccatgtag ggcatgtcct
    13441 atttgccatt gcgctttggg cagtaatgat ttatggtatc gaacgtatcg tccaaaaaaa
    13501 tgaaaaggtc aatcgttggg tcaaaggcga gccatctgtt ctaatcaaag acggcgtcat
    13561 caacatgaca gagttgacaa ataaccatat cgagatggag cagttgcgag caatcttaag
    13621 acagcaagaa tgcttttttc ttgaaaatgc caaacatgtt atcctagaga atgccggtca
    13681 aatgagtgta ttgaaaaaat cagatgaaga caaagcttta tcgatcctgc ttgtagatga
    13741 agggcaaatt cagcataaag tgcttcagtc caatcaattg actgaagcat ggcttatgga
    13801 gaacttgaag aaagaaggct atgcagatgt aaaacagctg atttatgttg aatggtcaga
    13861 agaaaaaggg ctctacatac taaataaaga agacgtcatg catgaatctt accgaataga
    13921 tggatagata gacagaggtt gtgaaatcag cgccttggct tgttactcaa aactaagcga
    13981 ctttttcaca acctctgata tctaatatac gttgttccag aagttgcttc ttcgaaaaat
    14041 aagccaaaat tccacaaaaa tttaagaagc aattttcgaa aaatcctttc ttattttttg
    14101 aagttgatca cttctgtcac aacctctcat gtcacaaacc atttttttgt ttcctttaac
    14161 tcaatatacc gtacgtcttc ccacaatatc ggctgatggt caatcataaa gtaatcttca
    14221 taggcttcac caatgaagtg cccttcgacc tcatccaaga gtcttccaaa ttcatctttt
    14281 aaattgagct gaatgattac tgctttacga gataaaaagg cttctgtcag tatactatcg
    14341 atttcttgca atgtcatttg ttgtttagga agaggctgtt tgcgtgcttc ttcttcattt
    14401 tttttgatac cgttgactag ttcttccatc gcaaaagcag tcccccattt taatccaaat
    14461 ggccgatctc gatactcgtt atagtcaatg aatttttttg ctgtccgtct catcttttcc
    14521 accttctaat ccactcatac cacctgcatg cccgccaacg agagaactac gtgcgatggc
    14581 gcgtcctcct tgcaagagac tatgcgcatg gatgattgat tgaaagccgt atttttttct
    14641 aatcgtatcg acaacaaaat caactttcgc ttcatttatc tgcttttcag gatcttcaaa
    14701 aaggttaagc tgtaaacctg tcgcgtatac gaggtcggaa cagttcacgc ctacacgccg
    14761 aatatcctgg tagtggtaat gctggtggaa aatggtcaat aaagcatctg ctagatctct
    14821 gctagcattt gtcgcttcga ttttcatttg ctggtgaaac ccggtcttgc catttggatc
    14881 aagatagcct aaagagtatc ctacccataa gctgactact tgtgtttttg ctgcagatcg
    14941 tctcaagcga gtgccgactt ggtcggccat ttctttgata acgatctcga tttcttctcg
    15001 tcttgtataa tctctatcta ggatttgact atttccgata ctttttgatt ttggtgtata
    15061 ttcttgtccc aaaaaacttc gatcgatgcc ccaagcatgg gcataaagct gagtaccgat
    15121 gacccctagc ttgcttttca agtgataata atccgcgtga gctaaatcat aaacagaatg
    15181 tatacccatt tgtttcagac gttttgctgt tcgtttgccg attccccaaa agtcagtgat
    15241 atctgagatt ttccataact tttctggtac atcttcataa cgccattcag caaacatttg
    15301 ctttccgtct tttgagccat tgtctaaggc tagtttagca agtagcgggt tatcgccaat
    15361 tcctatagtc gtgtagattc cggtctgctc aaacacatct ttatggatca gtttagccat
    15421 gtcgtacgca tttgttttac caaagagtgc catgctattt gttacatcaa ggaagctttc
    15481 atcgacggaa tagacatgat ggttcgcttc gtctgcatat tttttataga tagaattgat
    15541 ttctctgttc ttttccatat aataggccat ccttggggca acaatatata aatcacttgg
    15601 ataggggaag ggaagatctc tggcacggct gatattggtg atgccgtaag ctttttttgc
    15661 tgcaggactg gaagccaaga ttaatccgct accccgtgtt tctgtcgaat cggacggata
    15721 ggacatgact actaatttag ttgtaagggg atcaagatcc ttttcaacgc actctacgga
    15781 ggcgtaaaaa gatttgcagt caatacataa aatatctctt gaaggttctt tttcataaac
    15841 aaataccgga ttttgattcg cccacataa
//
LOCUS       DACECY010000060        15856 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.064, whole genome shotgun sequence.
ACCESSION   DACECY010000060 DACECY010000000
VERSION     DACECY010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.064"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            292..1431
                     /locus_tag="IWO38_002110"
     CDS             292..1431
                     /locus_tag="IWO38_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ0314188.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1498..2073)
                     /locus_tag="IWO38_002111"
     CDS             complement(1498..2073)
                     /locus_tag="IWO38_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0314189.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2078..3052)
                     /locus_tag="IWO38_002112"
     CDS             complement(2078..3052)
                     /locus_tag="IWO38_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ0314190.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3076..3120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3335..4303
                     /locus_tag="IWO38_002113"
     CDS             3335..4303
                     /locus_tag="IWO38_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0314191.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4416..4694
                     /locus_tag="IWO38_002114"
     CDS             4416..4694
                     /locus_tag="IWO38_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0314192.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4812..5048
                     /gene="secG"
                     /locus_tag="IWO38_002115"
     CDS             4812..5048
                     /gene="secG"
                     /locus_tag="IWO38_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ0314193.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5160..5906
                     /locus_tag="IWO38_002116"
     CDS             5160..5906
                     /locus_tag="IWO38_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0314194.1"
                     /translation="MKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSLAEH
                     QLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5909..8266
                     /gene="rnr"
                     /locus_tag="IWO38_002117"
     CDS             5909..8266
                     /gene="rnr"
                     /locus_tag="IWO38_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ0314195.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8288..8752
                     /gene="smpB"
                     /locus_tag="IWO38_002118"
     CDS             8288..8752
                     /gene="smpB"
                     /locus_tag="IWO38_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ0314196.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9054..9773
                     /gene="atpB"
                     /locus_tag="IWO38_002119"
     CDS             9054..9773
                     /gene="atpB"
                     /locus_tag="IWO38_002119"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ0314197.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9826..10041
                     /gene="atpE"
                     /locus_tag="IWO38_002120"
     CDS             9826..10041
                     /gene="atpE"
                     /locus_tag="IWO38_002120"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ0314198.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10132..10656
                     /gene="atpF"
                     /locus_tag="IWO38_002121"
     CDS             10132..10656
                     /gene="atpF"
                     /locus_tag="IWO38_002121"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ0314199.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10643..11185
                     /locus_tag="IWO38_002122"
     CDS             10643..11185
                     /locus_tag="IWO38_002122"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ0314200.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11208..12764
                     /locus_tag="IWO38_002123"
     CDS             11208..12764
                     /locus_tag="IWO38_002123"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ0314201.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12778..13680
                     /locus_tag="IWO38_002124"
     CDS             12778..13680
                     /locus_tag="IWO38_002124"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ0314202.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13703..15109
                     /gene="atpD"
                     /locus_tag="IWO38_002125"
     CDS             13703..15109
                     /gene="atpD"
                     /locus_tag="IWO38_002125"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ0314203.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15123..15545
                     /locus_tag="IWO38_002126"
     CDS             15123..15545
                     /locus_tag="IWO38_002126"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ0314204.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         5244 a         2863 c         3433 g         4316 t
ORIGIN      
        1 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
       61 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
      121 gtagaatagg aatctatggg caagtcctct cgtataaata aaataggagg gaagtagcag
      181 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
      241 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
      301 gaagaaaaaa tcgcgatttt acaagagatt atccgaatta aatcagtgaa tggaaacgaa
      361 ggtgaagtag cagcctattt gaataagctt ttggcaaggc atgacataac aggagagatt
      421 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
      481 aaagtgttag gcctatctgg acatatggat gtcgtggcgg ctggtgatga atcgtcttgg
      541 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
      601 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
      661 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
      721 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
      781 gagccaacca attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
      841 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
      901 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
      961 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
     1021 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
     1081 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
     1141 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
     1201 cctttgattc attccattca gcaacaattc agccagccat tacctttagt tggagcggca
     1261 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
     1321 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
     1381 ttggatatga tcgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
     1441 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
     1501 gcctattttt ctttttgaat atatcaagcg ttggggcaac atagtgatga ctaatgaaac
     1561 aacgacgata gacaacaagc gatcaaggta gtctgtaccc acctgtacca agatcacact
     1621 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
     1681 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
     1741 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
     1801 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
     1861 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
     1921 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
     1981 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
     2041 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
     2101 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
     2161 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
     2221 aacgatcaac caatgtttgt ccgcgactta ttccttgagt ttctacagca gtgaagcttt
     2281 taaagccttc acagatggac tcatcgataa aataagcaac agctaatgga tcattgatga
     2341 cacatcctaa gattctttct tgtttccagt gaaagtcaaa ataaaaacga gtgatcgctt
     2401 ttagatactc tccctcttca ggattcattt ggcagcaata ttcaaggata gtcggtgtca
     2461 agacgatttc tcttgtcaca tctaacccaa ccatttctat cggttgtttg agcttttcga
     2521 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggtgaacagt
     2581 taccatggct tttgtaagtt ccgcccattg agacaaaacg atccatatgt ttcccaacag
     2641 atgggctgtt tttcaaagca gaagcgatat tggtcaaagg ccccaaagca atgactgatg
     2701 tatctgtttt ttctttgaaa taatcagtca gaaaatcaac tgcatggagc gattctgcct
     2761 gtttggctaa gaccatcggg aaatttgtgt cccctaatcc atccaaccca tgagtgtctt
     2821 gcgcactaat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca gggatatcta
     2881 aacgatcaca gcgctccaaa catttcagca cattctgcgt accgatatct acagggacat
     2941 tcccgcaaac gacggtaata gcaactactt cgatatcagg ggatttgaca gctagtgata
     3001 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tactttacgc attatttcca
     3061 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     3121 acgagtgtct tgtttataat gcacgaaatt agtatactgg cttttattgt gaatgtaaat
     3181 gctctcttac aagtaaatct attgagaaga actttaagta aatgatacaa tgaaaaagaa
     3241 atggaattta cgtgtgccga agaaaaagaa tcctattgat cggtatattg tataataaat
     3301 agattatagt ttggcagaaa gcggcgggtg tacgatggtt attttttatt tcattcttat
     3361 tctgatattt ataggaatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     3421 aagagcgggg gtacagagag gctatgactg gtatgaagaa actgggcacc gcttgatgga
     3481 tcctgctgca gtcacgtcag tagaaaaaaa gcgacaaaaa ttagaagaag agtgtgaaag
     3541 cttctgggaa caaggaatac cgaaaacgat caaaagttat gacgggttga aactagctgg
     3601 acagatgttc ctccaaccaa ctcagcagaa taaatgggtc atttgtgtac atgattatcg
     3661 aagtacaggg aaaagagata tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
     3721 cgttcttata cccgatttac gtgcgcatgg agaaagtgaa ggagaaatca tcggaatggg
     3781 atggctagac cggctagatt taattgcatg gatccaactc atcttagatg aacaaccaga
     3841 tgcatccatt attttgcacg gcagttcgat gggcgcttct actattatga tggcaagtgg
     3901 tgaaaagctt ccatctgcag tcaaaggatt tatcttagac agcggatatg tttctgttta
     3961 tgcggaattt cggtatatgt taagtaaaat cacggtcttt cctaaaaaaa tgatcatgag
     4021 atatgcgaat cactatgctc agaaatacgc aggttattct ttaaagcaag catcagctac
     4081 aagacagctt ggtagtaacc atttgccttt gttagtcatc catggtgaaa gagatcattt
     4141 tgttcctaca gaagcagcat acacgattca aaacgctacg gcaggggata aagcattact
     4201 gcttgttcca gaagcagaac atttggaagc atcgatgaaa gatccgaaaa cctattggac
     4261 agtgattttt tcatttattg aacaaagaat aacgatggat tgattttctc ttcctttttg
     4321 taagcaacac gaggataaat aattgtttat agaccatgag actgttaaaa taaggtagga
     4381 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagag gaaaaaaatc
     4441 gtcttgcttt attcaatgat gagcaacaag aaatcggaga aatgacatgg tcggatgcgg
     4501 ggccagacat catgatcatt gaccatacat ttgtcgatcc agcatatcgt ggtcaaaaat
     4561 tagctgaaaa gctagtgtat actggagtag aactagcaag acgagagggg aaaaagatta
     4621 tcccttcatg cccttacgca aaaaaagagt ttgagaagaa accagagtat catgacgtct
     4681 tacgccaatc ataataataa cagctgaaag atttgcactt tcagctgttt ttccatttat
     4741 atagagttgt cacggagttt tatctatgat aaagttaaag agacgatatc ttttgggaag
     4801 gaggctgcta tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
     4861 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
     4921 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
     4981 caactgcagt catgggagct gtctggatga tcttattatt tgtcctagca tttttatctt
     5041 cgaagtagtc gaataagcct ctcgcacaac ggcgggcggc tttttttatt gcaaagagaa
     5101 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgaaacgag
     5161 tgaaacaatt accaaaaccc ctttatgcaa aacatggaaa acgagcagtt ttgctgcttc
     5221 atgcgtactc aggaagtcca aatgatgtcc ggatgctcgc acggttttta gaaaaagcag
     5281 attatacagt ctatgcgccg ctatttaaag gacatggaac gatggagcca tatgatatct
     5341 tgcaagaaaa agcagaaagc tggtgggcag atactaaaaa agctatccat tttttacagt
     5401 ctgaacagtt ttctgatatt gccgtattgg gattatcgat gggtggcatc tttgctgtac
     5461 gggcactaga agaagaatca gtaattggcg gcggtttttt ctgttcgccc ctgtctcctg
     5521 tagaaacaaa cgtgcctgaa aattttgaaa aatatgtacg tcaagtgctg aagatagcag
     5581 gcaaatcaga aaaagaaatc aatgaaaaag ctgtgtctta ccgctcgtta gctgaacatc
     5641 aattaatgga tatacaagac caagcagcaa tcgcagaaag tagactatca gatatccaac
     5701 agccaatatt tttagctcaa gctgggaaag atgaaatgat cgacccaaat ggtgtatttg
     5761 aaacggcacg caaattgagc cgccaacgag tgacacttca atggtatcct gaaagcggtc
     5821 atgtgattac agttggcaca gcaagacgcg aattagaaaa agacgtgttg gaatttttag
     5881 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acaataaaag agaaaattct
     5941 ctttttcatg gaaagcagtc gcaaacgtag tttttctatg gaagaaatcg ctgaaggtct
     6001 aggctttcaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
     6061 agaacagagt atcgttttca ataaaaaagg aaaagtcaaa cttcctttaa aaccgatctt
     6121 ggtcgaaggg acatttcggg caaatgaacg aggatttgga tttgtaacga ttgatcccga
     6181 agaagatgat atctatatcc cgaaagaagc gactggcttt gcaatggatg gggataccgt
     6241 agcaattgat atcatgaagc gagcaaatac agatatggat cgcggagcag agggaaaagt
     6301 agtggagatt cgagaccgag cgactactca gttagccgga gaatttgttg cttattcaga
     6361 cgaagagata caggaaacgg atctttatgg tgtggtcatc ccgaaagata gaaagcttaa
     6421 ccaattcaaa gtgtatgcag cggcagaggg agttcgtcca gttgacggaa gtattgtatt
     6481 gatcgaactg acccattacc ctgaaaaagg gtatgcaact agtttagaag ggataatcaa
     6541 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcgattg tcgtggctaa
     6601 cggtattcct acaaaatttc ctgatgatgt gttatctgag gcagatgaag ttccagacca
     6661 gatcagcgaa aaggatattg ttggacgcag agatttaaga gatcagctca tcgttacgat
     6721 tgatggagaa gatgcaaaag atctagatga tgcagtgacg gtcaaaaaat tagaaaatgg
     6781 taattatttt cttggagtac acattgcaga tgtatcttac tatgtgacgc aaggcagtca
     6841 actggacaga gaagcatacg aaagaggaac aagtgtctat ttgaccgatc gagtgattcc
     6901 gatgatccca caacgtttat ccaatggtat ctgttcattg aatccccatg ttccacgact
     6961 caccatgagt tgtgaaatgg agatcgatcc aaatggacaa gttgtctctc atgagatttt
     7021 tccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
     7081 agatcaagat gaagaaacga tgcaacgtta tgctcagctc gtgcctatgt tccaagaaat
     7141 gggtgaattg catcgaatcc ttgaaatgat gcgaatcaaa cgtggagcga tttcttttga
     7201 ggatcgtgaa gcgaagatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
     7261 aagcagaggg ataggtgaac gcttgatcga atcctttatg cttgctgcca acgaaacggt
     7321 agctgaacat tataataaat taaatcttcc gtttatttat cgaatccatg aacagccaaa
     7381 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
     7441 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
     7501 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
     7561 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
     7621 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
     7681 aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag atcatagttc
     7741 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
     7801 attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg ttgtgaaatt
     7861 cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca atgaattgaa
     7921 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
     7981 gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc cagatgaacg
     8041 agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac gtccaaaagc
     8101 acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct cagaaaaaaa
     8161 tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga aaaaagggaa
     8221 aaagccattt tacaaaggtg tcaaaaagaa gaaaaagaaa aaataaaatg agttggagga
     8281 aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag ctcgccatga
     8341 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg agatcaagtc
     8401 gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca acggggaagc
     8461 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
     8521 gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg aaaacgaatt
     8581 gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
     8641 ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag atttgaaacg
     8701 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
     8761 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
     8821 atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg tgcatgggga
     8881 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
     8941 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
     9001 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
     9061 aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt tgtataatgg
     9121 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
     9181 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
     9241 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
     9301 ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc ttacctaacg
     9361 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
     9421 taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa ggatatttcg
     9481 taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt gaagaattta
     9541 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
     9601 taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca ttagcaattc
     9661 cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa gcatttgtct
     9721 ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa taatactcgg
     9781 ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa ttatatcgca
     9841 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
     9901 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
     9961 atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt tatcgccttg
    10021 attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat cgtttgttac
    10081 tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc tatgctgaat
    10141 caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt tgtcagcggc
    10201 tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat cagcgatatt
    10261 ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca atctcgcatc
    10321 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    10381 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    10441 gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat cactgtagaa
    10501 cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct tcaaatcgcg
    10561 gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat caatcaatac
    10621 attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    10681 gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct atcaagatct
    10741 cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt taagtgatgt
    10801 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg gttatgatgg
    10861 aatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg accttttgtt
    10921 catgattgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat tgggaagtgt
    10981 cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    11041 aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta ttattggtgg
    11101 cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    11161 aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    11221 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    11281 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    11341 aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga atggcgcaaa
    11401 acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    11461 gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag gccttgatcg
    11521 gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc gttactgaca
    11581 aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct gttaacgaac
    11641 caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc ggacaacgtg
    11701 aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgattgat acgatcatca
    11761 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    11821 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    11881 atgccggtgc gtctcaacca gcaccattac tttatatcgc accatatgct ggtactgcaa
    11941 tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat gatctttcaa
    12001 aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca ggtcgtgaag
    12061 cttatcctgg ggatgttttc tacttgcact cacgtttatt agaacgtgca gcaaaattaa
    12121 gtgatgatct aggcggcggt tcaatgactg ctttgccatt tgttgaaaca caagctggag
    12181 atatctctgc ttatatccca acaaacgtta tctcaatcac tgacggacaa atcttcttag
    12241 aaagcgactt gttctacgcg ggtactcgtc cagcggttga tgccggacta tctgtctctc
    12301 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgtctag
    12361 acttagcaag ttatcgtgaa ttagaagcat ttacacaatt cggttctgat ttggatgcgg
    12421 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaactgc
    12481 atgcaccgct agctgttgag aaacaagtag tgattttgta tgccttgaca catggattct
    12541 tggacagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    12601 ataccaatca ttcagatatt tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    12661 aaacgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaaata
    12721 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    12781 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    12841 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    12901 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    12961 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgctgctc
    13021 agccgtcctg tgaagaaaac cggttatatc gtcatcactg cagatggcgg tctggtcggt
    13081 ggatataaca gttcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    13141 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    13201 ggtatcaacc tagcatatga gctgcgcaat ttatctgatc agccaagctt tgatgaagtc
    13261 cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga tgaattatac
    13321 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    13381 ccaatctcag acttagatcc agaagaagca acgactttcg aacaagaata tatttttgaa
    13441 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    13501 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    13561 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa ccgtgcaaga
    13621 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    13681 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    13741 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    13801 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    13861 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    13921 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    13981 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    14041 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    14101 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    14161 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    14221 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    14281 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    14341 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    14401 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    14461 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    14521 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    14581 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    14641 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    14701 agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    14761 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    14821 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    14881 aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa actttaacgt
    14941 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    15001 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    15061 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    15121 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    15181 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    15241 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    15301 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    15361 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    15421 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    15481 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    15541 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    15601 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    15661 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca gaggttgtga
    15721 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    15781 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaataaggga
    15841 ctgcgacaag actttt
//
LOCUS       DACECY010000061        15698 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.065, whole genome shotgun sequence.
ACCESSION   DACECY010000061 DACECY010000000
VERSION     DACECY010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.065"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="IWO38_002127"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="IWO38_002127"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HAQ0314205.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="IWO38_002128"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="IWO38_002128"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HAQ0314206.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="IWO38_002129"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="IWO38_002129"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HAQ0314207.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="IWO38_002130"
     CDS             complement(4028..5044)
                     /locus_tag="IWO38_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0314208.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5146..5343)
                     /locus_tag="IWO38_002131"
     CDS             complement(5146..5343)
                     /locus_tag="IWO38_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314209.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
     gene            complement(5315..6634)
                     /locus_tag="IWO38_002132"
     CDS             complement(5315..6634)
                     /locus_tag="IWO38_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011674482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAQ0314210.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
     gene            complement(7375..8355)
                     /locus_tag="IWO38_002133"
     CDS             complement(7375..8355)
                     /locus_tag="IWO38_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ0314211.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(8368..9246)
                     /locus_tag="IWO38_002134"
     CDS             complement(8368..9246)
                     /locus_tag="IWO38_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ0314212.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(9262..10062)
                     /locus_tag="IWO38_002135"
     CDS             complement(9262..10062)
                     /locus_tag="IWO38_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HAQ0314213.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            11454..11780
                     /locus_tag="IWO38_002136"
     CDS             11454..11780
                     /locus_tag="IWO38_002136"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0314214.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            11788..12696
                     /locus_tag="IWO38_002137"
     CDS             11788..12696
                     /locus_tag="IWO38_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0314215.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            13325..13576
                     /locus_tag="IWO38_002138"
     CDS             13325..13576
                     /locus_tag="IWO38_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAQ0314216.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            14118..14276
                     /locus_tag="IWO38_002139"
     CDS             14118..14276
                     /locus_tag="IWO38_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0314217.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            14348..15574
                     /locus_tag="IWO38_002140"
     CDS             14348..15574
                     /locus_tag="IWO38_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0314218.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
BASE COUNT         4618 a         3356 c         2656 g         5068 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaactt aagaaaacca
     5101 ccggagaatg aattctccgg tggacttttt gtatgattaa ccttcttatt gaacatcact
     5161 gatcttaaac tctattccaa gggcgtttct ataatattct ggatacatct ccttcccaca
     5221 tttttcacag gtaaattggg gaggattgtt cggatcattt gcaatatctg catcatctaa
     5281 aaattttact acatccattg ggattgattc tttttcacca caacttagac atataaattt
     5341 cacgttggcg ttcccaatcc cagcgaattg ctttaaaagt tttttctttt tcttcttcat
     5401 actgttgcct gaattctttt tggtcaattt ttcgattctc ctctcgcata aaccgctttg
     5461 catggttatc tttcgcatat ttaataacta accgtccatt tttgcgcaca ctcatcccaa
     5521 cgaattcata gtactcaccg caacaagaac attctaaggg attttcacca aattcttcgg
     5581 ttattctttc ttcccaggat tttggtctta aggcctcatt cagattgata aacagctttt
     5641 ttactcgttt ctgatactcc ttcatgacct ttttcattaa tgttttgatt ctacgactat
     5701 agattccata gcgacgaatc gttttgaaat tcttattttg aatatggcga atcagtgcag
     5761 ctatgaattc gtctatactc atggtcatcg tctctttagt attcgttcgt ttgtccatgt
     5821 agttgaacat gacagtgtcg ccatcataca ttaagattcg gcttaatgcg atcgggccac
     5881 gcttcatata acgactgatg tattggagta gtgactttag atttgtcctg ctccttttag
     5941 gagcatttac gtaaaaccct tctccattgt tcttgtacgc agcttgtaat cttgattgaa
     6001 ctctttgctt gtcccactca cctaacgttc gtctaattag tttcaataca gcatttttcc
     6061 agtattttct taacatagaa aaaggtaaat aatcgtattc ttcccaactt ccatctcttg
     6121 ttaagccacc catagtaaca accatatgaa catgcggatt aaattctagt tttgatccaa
     6181 aggtgtggat tgtagctact atacctgcct tcacctttct tttctcaaaa taggcaagaa
     6241 taatttttgc ggcttcatcc attagtcctt tcagtaagga atctcgatag ttttccatca
     6301 taaagatttc ccgtaacccc tcatcgatcg taaagattac gtgccgatgt gtcactttaa
     6361 acatatcatt cgccacaacc gctgaccacc tttggctttc acctgttgca cacgttggac
     6421 agaactttcc acgacatttc aagggaacaa actttgtttg atgacatccc tcgcagacaa
     6481 acagtcggta tcctttttga atatctccac aatatctaaa cttgtttact tcccgtttga
     6541 cgactagccg aatctttcgc ccgtatttct caacaaagtt atcccaatta tggtgttcgt
     6601 caaaaaagat tgcttcgaga atattcgctt tcatagtaat attttacttg ttcaaaaaca
     6661 aaatgaaaag ggctaaaaga ctggtagtgc agccttttta gcccttaact ttttatactt
     6721 tctttactgt ctaaaaaata tgcggcgcac agaacgcgcc gcatatcgga ctaagtttta
     6781 ttcaaatttg tctccgccaa cacaatttga cgaagagcct tttctttcag gaagacttcg
     6841 gtaattttta ggagttctac ctattctttt aaccgtttat tctctttttc taagcgtgta
     6901 atttcatttc tgcgaatgga tttcatgact tcctctaccg agaaaagtac gttatccata
     6961 tttttcaact tcttgaactc atcaatacac gctattagag cgagcttcta gctatagact
     7021 aacttcttta acagaaaacc cttcttccgt taccagtttt acagcggtaa ttttaaatat
     7081 ttcatcaaat gaacgtcttg gcataatcca gatcttcgct ttctcttaat ttggtgtcaa
     7141 ttttagtata acccgtccaa cttatttttt aactaagtgc ccggtttttc ttgacaactc
     7201 cacccaaaag ttagattttt tggtctaagt tttagggtcg cttcattact agaattcaac
     7261 aagagatgtt ttttatttta tttaaattat ggggtcagtt tccatttgat tacaaacttc
     7321 tttgttttct ttttttaaac gcaataataa aggttttaat ttcagccttt ttaattagaa
     7381 aatcaatttt cccaaattat tttctttagc tttcttaacg atttctgtcg caactaccac
     7441 atctaataca gccgaaccaa ctgttttgaa gatcgtgatt tgttctccac ttgtccgacc
     7501 gatcaattct tcattgatca gttggcctag ttccccatga tagctgtctc tttgaatata
     7561 cccatcttgc agcggtgaaa tgaagtcccc agcttctttt agaacaccgt ccatggtatc
     7621 aaaaataaca acatctgcgg ctttgattat ttctcgtgga atttcacaca tttccggtgt
     7681 gtaagcaccg actccattga tgtgcgcacc ttttttgacc cactcagcag agaatgtcgc
     7741 acgttttgaa gtggtcacac ttgtaatgat gtctgctcct tcaacgcatt cttgactggt
     7801 ttttgttggg cgcatcgtta cagaaaattg ttgcatcatt ttttcagcaa attggctggc
     7861 acgctcaaaa tcaatatcaa aaatccgaac ttcttctaat ttacgaacgg tcaacattgc
     7921 ttctaattgt gactgtgctt gtccgccggt tccaatcagc gcgccaattt tagcatcttc
     7981 tcttgcaaga agttctgttg ctgctccttg aacagcgcct gttcttaatt gtgtcaaata
     8041 agtaccatcc aaacaagcgg ataccatacc cgtttctgga tccaatacga tcatcgttgc
     8101 tggtacactt ggtaagtttt tcttgatgtt ttcaggatac acggaaacga ttttgacacc
     8161 tagcgcgcct tcgccacctt ctacataagc aggcatatat aagctttgtc cgttgctttt
     8221 tggcacatcg atattggtgc gaagaggcac acttgccttt cctgctgaat acaatgacag
     8281 tgcttttttg tccgcatcga tggcttctct cattgaaaaa cttttgataa tatcttcttt
     8341 tttaagaaat aacatttgaa tcgctcctta attagctgct gtaacttttt ttaattgact
     8401 gcccaacatc gtatatgcac tgtatagtaa gaccaaaatc acgatccatt tcaggatata
     8461 ggtatctaaa gatttgacta ggaatacagc gaccaatacc cccaatacac cgaaggttga
     8521 agtaaaaagt gtgatttttc tgctgtattc attaaattta atgaattgaa cactgccgat
     8581 cggtactgag aaggtacaag cccccatcat aataggaaag gctgcaactg gattcaagcc
     8641 tagcaagtag acagttacca tcgttaacgc ataagaacca atgccgatat tgttcaatgc
     8701 accatagaca aagagtaaaa atcctgccaa gattaatttt ccgccataca attcactggc
     8761 tgttccgttt gatggaatcc agttcatttg tcctgcaaaa atcaacaatg cagcaatgac
     8821 caatcctatg ccgacaaaca gtttgatcgt tttctctggt aacttcacga caaatcttgg
     8881 tcctaaatat gcaccgataa tttggcagat aatacaaaca gctagtgtct tgatccctac
     8941 gttgatggca gtgatgtaag acaatgccat gacggctacc ggtactacac attgggtatt
     9001 caacgttccg ggtagttttt tcatgctgac ccatttcaac tttggataaa ggacggttcc
     9061 gatagcaaag tcggaaacgc caaatgttga aagaaagaag atgataaatg acgtgaaggg
     9121 caacagacgc atatcccctg gttcttcttt gaaattttgt ttgtttttga aaaggtctct
     9181 tagaaagtaa ataataaaat atagattgat tgcgacaatt agtgtcagca aaatattccg
     9241 aatcataaaa aattcctcct actagtaaat ctttctgatt ccctcaaagc cctcaccata
     9301 ttttgcttta agttcttcag gagattctgc aatcactttt agtgtatatg catcatgttt
     9361 gacatattct tttgctagat tgaccatggc gtggtttttt gtccaagtga catcggttga
     9421 ttgcagttct tcgttaatgg taccaaacac gacttcttgc tcatcggcga cgatgttgat
     9481 ccatcttgct ccaaagtcag caagtttgtc tgtttcaaaa ccatgaatat aatacttttc
     9541 taagggaaga tcatattcat gggtagaact gaataaaatc agtacaaact tatcaactct
     9601 tttttcagct tgctgcaatg cctttagtag tgaatccgtg aaattctcat gccaaacttg
     9661 aatgagcaaa ctttcttttg cgtttttcac caaatgctct actttatcca gaacatactc
     9721 gataccatca actttccaca gcatttcttc ttcatctttt tctttgatca tccctagatt
     9781 cttggtaaga aaagaaagat catttttaac acttttttct aatttctcta aaaactcatt
     9841 tgccgagatg gcatgataaa gtttaggttc tgatttatta acgacaatca aattcttttt
     9901 taatagtttt tccaaaatat tatatacttt cgagcgtggc acacctgaga ttttacttac
     9961 ttcatatcct gtaagattac ctttttctaa tagtgtggta tacactcttg tctccatctc
    10021 tgaaaaatca tattttttca tgatttgaat aatcgtttcc attgattaat cctcacttta
    10081 tttttagtag ctactacggt aacaactata aattcttttt gattatctgt caagagttag
    10141 ttcaataaca cacatataat aattagctta aaaacaatct ttttattaaa aagaaacatt
    10201 ataatatgaa tgtttgtgaa aaaataatac aacttagtca ttctttacaa cgatttatag
    10261 aaaaacaacc tctttaaata taaaaggtac gggttactga ttacttgtaa atctttacgt
    10321 tccagtttta ttctttctaa aaaatatact cacttctttt cttagacttc tttttttgat
    10381 tctcctcttt atggtcttgt ccttttttgc cttcaaaata tttaaataaa cccctttcaa
    10441 aagcatttct aggttttgga caataaggaa tgactttttt cttcatataa atacctcatt
    10501 taactatttg atatcatcac attatcaaaa aaaataggtt ttttattaaa actatcttga
    10561 cctttcggga gatttcactg tgatttattt ttattcccat acttttttcg ttggtcactt
    10621 tcgtatacct aatctaagcg atcatcggcg acggtgcttt tacaacgaat ggcaggacaa
    10681 aacaaggggc ggaaaaactt ttttgcctat ttcataaaaa actttttcct acagcaatcc
    10741 cttgttttgt cctaccacac gttgtgaacc caaagcacgc cgacgaacgc ttagctatac
    10801 gaaaatgacc tccaaaaaca aaaaagtgag gtgaatagaa acaaaaagaa attgaaatct
    10861 cccaaaatgt cgtccgcctc ggagacaact tgctgctgaa aatggttggt taattcattt
    10921 aagtattaaa taagataaat catcaaatta atcggaaaaa tagcatgatt ggccttatca
    10981 aaaataaact gtaaaaactt atatttctat gagtggtgga acaatgctaa taccaaatta
    11041 atttatcgat tacccacctg cacaaaccaa agatagcatt attaggaaac gaatcaatca
    11101 gcgaatccag cattataatg aggatactaa aaataaagtt ttcataatta taaatccgcc
    11161 ttcctacgaa aggcaccaat gaggtgatga aaaccttaga gcgctttctt tcttttttta
    11221 ttccgtgtta ccctttcctt gcaggtccct ggtacactac agaacccgag gaggtgagtg
    11281 gcggggctgt atagcggcaa ccccgcataa ttatatggtg tcggtcatcc gttaaggcat
    11341 caatgaatgg cgcaaacccg tagcccgtaa acttatctct tccgaagttg actcgatttc
    11401 gccggatgac cagtccctac caaacctgta ctattttttt acaggaggtt gctatgttca
    11461 aaatctttcg taaaaattgt tgtggcttgg atgtccacaa aacgtggatc tatgcgtgta
    11521 tcggaatcac agatgcgaat ggtcgtacag tgtataagca agctcgcttc tcttccttta
    11581 caaaaggtct caatgaatta gctgaatggc ttgctaaata caactgtaag gatgtgtgtt
    11641 tggaatcttc cggtaaattt tggattcctg tttttaatat tcttgaaaaa acttgttttg
    11701 tcacactggc tcatccgaag tacaccaaac cccagaaagg aaataaaaca gatcgcaaag
    11761 atgcaaaaat ggatttgtga tctctttatg tgcgacatga tcaaacccag ttttattcct
    11821 ggtcttgaaa tccgccagct tcgtgatcta atgcgttatc ggacgaaact gaccaacatg
    11881 aaaacaagtg aaaagaatcg tgctttaaac tgcttaaccg tttctaatct gaaactagat
    11941 gatgttttta gtgatgtgtt cggaaaatca tccacttcta tcatccaata cttgttagaa
    12001 catcccggtg aatcatttga tgttcgacca tttatccatg cccgttgtaa aactcccgtt
    12061 gaagaaatcc aagcggcagt tgagggggcg atttctgtac ctcaagcgat taagcttcgc
    12121 caatgcctca accatattaa cgagattgag aagcatcgag aagagatcaa tctagagatt
    12181 caagctcttg cagaaccctt ctcccctgtt ttggaactac ttcgtaccat accgggctta
    12241 gacactcagc caataacagc gattgcgatt ctttcagaaa taggaccgga tatgtccgtg
    12301 tttccaactt cgaaacactt tatttcttgg gctggatgct gtccaagaaa cgatcgcagt
    12361 gcccgacgaa caaaatcaac acgtatctct cgtgcagggc agtcgttgaa accgctactt
    12421 gtacagattg ccaatgctct tctcagatcc aaagaacatc cagaatttaa aaatcgctat
    12481 cgtagaatca aagcgaatcg tggacacaag aaagctatca ttgctatttg taagatgctc
    12541 ctgaccgcta tctggaatgt cttatcaaag gtggaaccct attctgctaa aggatatctg
    12601 gagccaaaac ctgcgaaaga agagaaagtt ctgacgcaat cccaagctct ggacctttta
    12661 agaaaacgcg gatataccat tatagatagt gcttaaacca aagttacgtt gattcttcct
    12721 aaatagactc gttgtcttcc atttcaaaaa ttttgaaaac ggtaggctta tttagtgtac
    12781 cgttcttcag gtatcttcaa atagatttat tttcaaactt atttcctttc ttcttttact
    12841 ctcctatagc ttagcattta agtgggcact cgtgtcacct tgctagatag ctatgaatgc
    12901 cgtaataagc ctcctagtat ataggcagac tgccaattat aactgattca atccacatta
    12961 tgataaaatc caccttttcg tttcatatgt gttgaaatat cacatatgtc aaaagtgcac
    13021 gagtcttttc attcagtgct ttgtaaatac tcaaaattat tccttttcac tttaaaaaaa
    13081 agataattag tacaaaatca gaaatgagga aagtattagg atggactaga aaggagttgt
    13141 tgaatttgtt gcaacagatt ggtcatttat caattaaaaa ttaggtgaag ttttattcat
    13201 cactgcagct gctgtttccc agagaataaa acgtctagaa gaaactggtc agttactgta
    13261 ttatcaagca gcaattgact acgaacaagc ttgattacta accaaagcgt ttatccacct
    13321 gttgattaaa tcaaatcaaa aatcaggttt ttatacgttc ataaaagcaa tctccagtgt
    13381 actcgaatgt gactgtatca taggtcctta ttcgatgtta atcaaaatgg ggtttccttc
    13441 taccgaatca ttggacagtt ttatcaatga tttatcaaag tttggacgaa ccaacaccca
    13501 aatcgttttc tcgactcttg tttccttaat ggtttatcag ttctggaatg tttatcatgc
    13561 gcttttatta aaatagaaaa gagctttttt catgcgacaa agcagtgatc atttattggc
    13621 acatgcaatc tttgtggatc cattttttga aaatacgata tttagttttg acccaaaaat
    13681 ctttaaatac gtatttgata atttatctta acaaccagat aattttattc agcaccgtat
    13741 tatctatatt gaattctcgg aatcaagata aatttttttc cgtttttgtt tcttttttaa
    13801 tcttcttctt gttattaata gtgattagga tgatattcct atattgatct ttgataatta
    13861 gatataattt ttgaataacg ttccctgcta ttcgagctag aaatacaagt acgccatgac
    13921 ctagttagcg acaactactt agagatttct tttgttctgg gacaacaaaa tgccatgaca
    13981 atagcagtaa ataatgatac ttccgtaagg ctcgccgtaa actggggaga ggtgaaattc
    14041 ctatgtgctt acccaaagcg gttcaaaaat tttaaactat tattttagga gatgatttta
    14101 taaaatcgaa agcctacttg aataaaaaac aagcctgtga atttctagga attgataata
    14161 atactttaga taagtggatt cgtgtatata attttccagt aataaaaatt gatggcgtag
    14221 ttcgctttga cgtagaggat atcaaagatt tcatgaatac attcaaaaaa aattgaaatt
    14281 ttcttctcgt cacatatact ataaatagcg aaatgtactg ccttactttt taaaggagtg
    14341 aggaagtttg cctacaaagc tttcaaatgg gaaatataaa actaatctga ggtatccaaa
    14401 aaaattcaga gaaatcactg gaattgcatc agagaagtat caaaaagtat ttccaactag
    14461 gcaattagct attaaggccg aaagggaaat gaaaaagaag attgaaacag ttttaagaga
    14521 ggagaatgca aactcactag aattgaaagg aaaaataaaa tttaaagatt tttatgaatc
    14581 aaaatggctt cctagatatg aactaggtca aactactagg tcaaacaggg ctccaagtaa
    14641 tgtaacaatt agtaatacta aggatatatt ccgattgcac atccttccaa tgtttggtga
    14701 atatgcaatg aattatctga attcaaatac tgagataata tcagatgaat taacaaagaa
    14761 atcgaaagaa tacgcaaata taaaaattat caaaggctat gttcgctcta tgtttgatat
    14821 tgccgaaata ctatgttata tcgaattcaa ccgaactacc aaaattattc aatgtattac
    14881 cgcgcctaag aaaatggctt tagaagaaaa aaggataagg gaagggaatc aagcattatc
    14941 atcagaagag ctgactgatt ggatagaagc agtaaacaat gacttaaata atcacttgct
    15001 tacacttcac gattacacac ttttcatgct taccttatat ctaggtgata gaaaaagtga
    15061 gacttatgct ctgcaatgga aatatattga ttttaataaa aatacggttc gtctcaaata
    15121 tgctttagat aaatatcaaa gaaagacaca tacaaaaggt tggaaagaca ccgttattca
    15181 ggcaccagaa gttgtgatga cattattaag agaatggaaa tcagctcagg ctgaacaatt
    15241 gttgaaacta aaaattatac aaacacctga ccagtacctc tttacctatt ccaaaccttc
    15301 tggagaagta aattgtcctg ttcacgctga ctacttaaac tatcgaatca atgcaataaa
    15361 aaaaaggcat cctgaattgg ctcaattatc tccacacaaa cttcgtcata cctatgcaac
    15421 tatagctagg gaaggtggag caaatatgaa tcaaatctct aatgctttaa cgcactcgga
    15481 tatttctact acaaaaatat acgtgaacac acctgacgta gttgataaat ctgtttttga
    15541 agcatttcaa aaaggattga ataagtgtga ttaacatgtg ttctcaaatg tgttcttatt
    15601 ataaaaaaag acactttcca aataattgaa aagtgtcctg aaatattgat ataaaaagaa
    15661 tattaacgtt ttgagaattg tgaagcttta cgagcttt
//
LOCUS       DACECY010000062        14966 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.066, whole genome shotgun sequence.
ACCESSION   DACECY010000062 DACECY010000000
VERSION     DACECY010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.066"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..596)
                     /locus_tag="IWO38_002141"
     CDS             complement(<1..596)
                     /locus_tag="IWO38_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0314219.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKL"
     gene            869..3340
                     /locus_tag="IWO38_002142"
     CDS             869..3340
                     /locus_tag="IWO38_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0314220.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3443..5179)
                     /locus_tag="IWO38_002143"
     CDS             complement(3443..5179)
                     /locus_tag="IWO38_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ0314221.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5176..5880)
                     /locus_tag="IWO38_002144"
     CDS             complement(5176..5880)
                     /locus_tag="IWO38_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0314222.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6011..6514
                     /locus_tag="IWO38_002145"
     CDS             6011..6514
                     /locus_tag="IWO38_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ0314223.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6474..6812
                     /locus_tag="IWO38_002146"
     CDS             6474..6812
                     /locus_tag="IWO38_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ0314224.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6833..8251
                     /gene="ffh"
                     /locus_tag="IWO38_002147"
     CDS             6833..8251
                     /gene="ffh"
                     /locus_tag="IWO38_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ0314225.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8363..8941
                     /locus_tag="IWO38_002148"
     CDS             8363..8941
                     /locus_tag="IWO38_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ0314226.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8945..9466
                     /locus_tag="IWO38_002149"
     CDS             8945..9466
                     /locus_tag="IWO38_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0314227.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9545..10099)
                     /locus_tag="IWO38_002150"
     CDS             complement(9545..10099)
                     /locus_tag="IWO38_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0314228.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10352..10627
                     /gene="rpsP"
                     /locus_tag="IWO38_002151"
     CDS             10352..10627
                     /gene="rpsP"
                     /locus_tag="IWO38_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ0314229.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10639..10890
                     /locus_tag="IWO38_002152"
     CDS             10639..10890
                     /locus_tag="IWO38_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ0314230.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            11015..11806
                     /locus_tag="IWO38_002153"
     CDS             11015..11806
                     /locus_tag="IWO38_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314231.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11976..12497
                     /gene="rimM"
                     /locus_tag="IWO38_002154"
     CDS             11976..12497
                     /gene="rimM"
                     /locus_tag="IWO38_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ0314232.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12487..13248
                     /gene="trmD"
                     /locus_tag="IWO38_002155"
     CDS             12487..13248
                     /gene="trmD"
                     /locus_tag="IWO38_002155"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ0314233.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13384..13731
                     /gene="rplS"
                     /locus_tag="IWO38_002156"
     CDS             13384..13731
                     /gene="rplS"
                     /locus_tag="IWO38_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ0314234.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13989..14282
                     /locus_tag="IWO38_002157"
     CDS             13989..14282
                     /locus_tag="IWO38_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ0314235.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         4978 a         2708 c         3035 g         4245 t
ORIGIN      
        1 agtttgtaaa gtctgcgatc ttcaaggatc gtaaatagct gattggttgt cccatcaaca
       61 caaataaaac tcatggaacc agaaacagaa ttcattgatt tgaactcatc catacacaac
      121 accgtcggaa ggaagttcat acgtggctgg taatgcttcg ttgcttcacg caagatccgt
      181 gtcaccgtcc catccgagac gaagtacttc ttagcgatta gtttacgtga ttgattttcg
      241 tacagttcca tgagaatcgc ctgcttcagt tcctttgtta gttgatgatg ttcatcaact
      301 aagtccgttt tcgcagaaaa ggtccttcca caatcatggc agagaaaacg ggtacgtttt
      361 aattctaaaa tagttaactt ttctttgact ttcaacatct gagtgcgtgt cgtataggaa
      421 ccgttgcgga taatacgaac cgatcgacag tgttcacaat aggaagaaga gtcatccaat
      481 tttcctttaa taaactgcgc ttgaacgcct ttatattctt tttcaatcag ccattcttca
      541 tcaaaaaata aatgtttatc tgttaatccg agtaattttc tagtatgatt atccattgag
      601 agactccttt ggtaggatga cgttttggtc gactaaattt taccaaaagt gagtctcttt
      661 ttgagttgaa acgaagaaaa agtgttagta gaaaatcgcc ttcgattttc caccaacaca
      721 aaatattata gaaccctttt tttttgctat atgacaggaa tgaaaggtgt ttgcaagctt
      781 aatgtgagga acactttcca tatctgttct atactggata ggagaagttt ggaaatatcc
      841 agaaaacgaa atcaatcgag gtagccacat gaagaataaa tggataaatc gaagtaaaaa
      901 aaagaaagta acacaaagat catcagataa agtcatccgt ctttttccat cgtataaaga
      961 aggattaacg gcagatcaag tcaaggaacg aatagaaaaa ggtgcagcaa ataattcagt
     1021 cgatcccaca ttcaaaacaa atcagcaaat cgtattggaa aacatattta cgtatttcaa
     1081 tttgatcttt cttatcttag ccattttgct ttgtttagta gaatcctata agaacttgac
     1141 ttttttacca gttatcatag ccaatacagg aatcgccatt tttcaggaaa ttcgatccaa
     1201 aaagatcttg gacgaactaa atgttttaaa tacagcaaaa gtcaatgtca tacgagacgg
     1261 gatagaacaa acaatagata tagaagacct tgtaatagat gacattgtta ttttagagac
     1321 tggatatcaa attccagcag atgcagaggt cgtggaaggg aaactgcaag ttaatgaggc
     1381 tttattgaca ggcgaagcag atgaaataac caaagaaata ggtgatccat taatgtccgg
     1441 cagtttcatt gtttccggaa aagcttacgc ccgattagac aaagttggag cagattcgta
     1501 tatttcccag ctgacagtca aagccaagac aatgggagaa ggagaacagt cggagatgat
     1561 tgcttctctt aatcaattga tcaaatggat tgggatcatc attattccta tcggtatcct
     1621 gcttttttct caaagttact tttttaatgg aaacacgatc aaagaaagtg tcgttgcaat
     1681 ggaagcagca ttgatcggta tgatacctga aggactgtac ttgctgacga cgattgcatt
     1741 agcattaagt gcgactaaac tagcaaagca aagagttctt ttacacaata tgaaaagcat
     1801 tgagacattg gcaagggtaa atgtcctttg tgtagataaa acaggaacga tcacagaaaa
     1861 taaaatgtct gttcaaaaaa tgatcgtctc taaaaaacaa gaaataatca gtcagtcaga
     1921 tcaattggaa gcatgcattg ctgactatgc aaaagcgatg gcaggagaca atgcaacaat
     1981 ggaagctgtt aaagcatatt ttacaaaaac cactgatcaa tcctatcacc atgttattcc
     2041 tttttcttct gtccaaaaat tcagcagtgt agcgttggca gacaaggtgt atgttttagg
     2101 agcgcccgaa atggttttgc gcgatcaatt gccggaatat gcggaagagt ttgtttcttt
     2161 cgctgaaaaa ggctatcgcg tgttggtctt tggtgtgtac gatgggattc ttgaagaacc
     2221 agtattgaca gaagcagtca caccattagg ctatattctg attgctaatc ctatcagaaa
     2281 agaagcaaga gccacatttg attacttcaa aaaacagcaa gtagccatca aagtgatttc
     2341 tggagataat ccgctcaccg tatccaatgt agctgtccaa gcagggattt cacatgctga
     2401 tcagtacgtt gatgcatcta aattatcaga agaagaatat gaagcagctg tcgaaaaata
     2461 tacagttttt ggacgcgtca agccagaaca aaagaaaatc ttcgttcaat tgataaagcg
     2521 taacaataca gtagctatga caggtgatgg cgtgaatgat attctagcca tgaaagaagc
     2581 agactgcagt atagcgatgg cttccggaaa tgaagccgcc atgcaggcct cacaagtggt
     2641 cttacttgat tctgactttt cacgcatgcc agaagttgtt gcagaaggac ggcgcgttgt
     2701 gaacaatatt gagcgatcag ccagcttgtt tttagtgaag aatattttct cctttttgct
     2761 ctcactgttt tcagtgatct ttgctctgac ctatccttta gaaccatcac aaatcacatt
     2821 gattagtttg tttactatcg gacttccatc tttcttgctt gctctagaag aaaacaaaaa
     2881 gcgaatcaga gggaaattca tcatgaatgt gatggaaaaa gctgtgccag gtggactaac
     2941 agatatgatc gtagtcggtg cgttggtgat ctgcggggtg actttgaatc taaataaaac
     3001 agatgtttct acagcatcta ccatgctttt gattgcagta gggttcctcg ttctctataa
     3061 aatctgttca ccgctgaata aattcagaag tcagatcatt cttttctgtg ctagcggtaa
     3121 ctttttttcg gtcatctttt tgcataagct gttttcaatc accggtatct cagctgtatc
     3181 acttttgctg ttgagcattc tatttttctc ggcagattcg atttttaggc atttgaccac
     3241 attggtagaa tatttgtttg aacgtaaaaa tgaaaaagag caaaagccgt ttagtcgcgt
     3301 agctaaaaaa tttctatcga tatttaaacg agaaaattga cgaaaagtat ctcaaaaaag
     3361 agtaaaaaag aacaaccact cgtgtatctt tcgaagcagg atgagtcatc caactttaaa
     3421 acgcacgagc agttgctctt ttttacattt tcggaatggt aacggtgaaa gtcgaacctg
     3481 ttccaagatg actgtctaca gtaatcgtac cattaaggag ttgcacgtag tcttttacaa
     3541 tcgctaaccc taatccggtc ccgcctgaat gtcggcttcg cgccttgtct acgcggtaaa
     3601 aacgctcaaa gatccgttcc tgatcctcaa tgtcgatacc tataccaaag tcttggacag
     3661 aaaacgatag atcatctgtt gcctgatagc ggataataat ctcgctgtcg ctttttgaat
     3721 attggacagc attttcaatc agatttttga taattggata aaggatatct gttttagtag
     3781 taaatggttc gttctttcca atcaatcgaa tcgtcaaccg tttcttttcg atgattggct
     3841 gataattttg aacgatttgc tggaaataag gttctgtata aattgtctgg atctcgtaag
     3901 aattctcgcc atcttttgac agctggatga tttcccgaat cagtttatct aaccggatcg
     3961 catctttttg catgatctca agaaaagaag tcagagtttg cgggtcatct tttgcaccat
     4021 ccagtaaagt ctcggtaaaa ccaatcagtg aagtcacagg agtttttaat tcgtgagaga
     4081 cgttgccaac gaaatccttt tgtagctttt ctaaacgtcg cactttcgtt aagtcatacg
     4141 ccacccctag aatctgtccg gtaccttctg aattagtaaa gtagcgcaaa ctcaagtcta
     4201 atgtctgccg accttctgta agggtgatct cctgatggat cagcggatgc tcaggagtca
     4261 cttgatgaat cagctgaatg agttttggct cttgaatgac ttctgtgtac cgctgttcag
     4321 gttgatagct taccacgttt aaatgtagct gcatctttgg attaagcagt agtaaacgag
     4381 aatcagcatc atcaatcagg aatacgccaa tcatcagttc gttcagtaac gtatataatt
     4441 gctcttctgt ggtagtacac gcccgataca tcttactcat ttgttcgctc aacgcattaa
     4501 tcgtctgata caattcttcc cattgatcgg gtgtttgcat gattacttcc gcctgattag
     4561 gattttgcac catttttttt agtacgggca gcacggtttt caatggctgg tttttttgat
     4621 agatcagata atagatcatt cctgcaagaa caaggaagaa agacagaaag aagaaaaaga
     4681 cccaacggcg aaaactctca gttctcggta aaaaaacgct tgtcggttct gcgatacgta
     4741 aaatggcttc taactggcca ctttttttga ctggtaatgc gacatataaa agatcctcat
     4801 tcaaagtagt acttttgcgc aaagcgctac ctaggttccc accattcaat acggctttga
     4861 tttcgggacg ggagtttcgc tgttcattca atgtttcatt gtttgtgtca aataagatct
     4921 ttccagtagc atccattaaa gtaatccgtt cgttcgactg atggacgaat tcttccaaag
     4981 cagttttgtt ttgcgctgct tggaggtttt cgacatctaa ttgccggatc agcagttgtg
     5041 ctttttttgt caggtagctt tcttgctgtt ccacgatctg ctgttgaaaa taatgggaaa
     5101 tcaattgcca gccaccaaaa aacaaactaa ctaaaagaat aagaaagaag agacgttcat
     5161 aaattttttt ccatttcatc gtttgggttc ctgaaactta tagccaaatc ctctaatagt
     5221 caccaagtac tttgggtgtt ttggatctcg ctcgattttt tctcgcaaat gactaacgtg
     5281 tacatctacg attcggcttt gtcctgcgaa gtcaaaattc caaatgcggt ctagcaatgt
     5341 gtctcggtcg atcacacggt cttttcgttt catgaaataa acaagtaatt caaattcttt
     5401 cggtgttagt tcaatcagat ggtcgtctac ggtcacttga taattggtca aatcagcagt
     5461 gatttgtcca atcgatagat attcaggctc agcttcatct tttgattgct tacgaggttc
     5521 aattcgtctg aatattgctt tcatacgggc taatacttcg cgaggactaa atggtttggt
     5581 caaatagtcg tcagcaccta tttctaggcc aatgatccga tccacttggt catctttagc
     5641 cgtcagcatc aaaatcggtg tttcattttt ttcttgacgc agccgctgag tgatctccat
     5701 gccatccatc gttggaagca tcacatctaa aatgatgaaa tcaaatgatt gttctaatgc
     5761 taattctaat ccttcgcctc catctgtggc actggttacc ttgtagcctt ctttttccaa
     5821 attgaacgtc aagagcgtta caatagaagg ttcatcatca acaactagta tttttttcat
     5881 tcaaatctcc tttaaatagg tttcattctg tttatatttt agcatagaat cagtacaaag
     5941 ggctatttta caaaagaaat aaatggtata ataggaaggc actttgctga atagaaagga
     6001 agagcagtca atgattggaa tcagtgcatg tttaggcggt ctggcctgtc gctatgatgg
     6061 acagaaaaaa gaagtaccag aattgatgga actagttgaa actggagaag ctgtcatggt
     6121 ctgtccagaa gtgatcggcg gattaccaat tcctagagat cccgctgaaa tcattggtgg
     6181 aaatggattc gatgtgtgga aagatcaagc aaaagtatgg acgacaagcg gcgaagatgt
     6241 aacggaagcg tacaaaaatg gcgcgatcaa agcctatcaa aaattgcagg aaaaacacat
     6301 tgatctagtc atcatgaagg aaaaaagccc ttcatgcgga acccattcga tttatgacgg
     6361 aagtttttcc ggaataaaaa aaaatggtgc cggagtggct actgcttatt ttattcagca
     6421 aaaaatgact gtgctctctg atgaagaatg gctgaaaact agaggtgagc agaatggaaa
     6481 tcgaaaagac caatcgaatg aacgcgctct ttgaatttta ttcgacctta ttgacagaaa
     6541 aacaaatgaa ctacatggaa ttatattatg cagatgattt ttcattaggt gagatcgcgg
     6601 aagaatacga agtaagccga caagcagtat atgacaatat caagcggaca agcaagatct
     6661 tggaggatta tgaaaaaaaa ctccatcttt tttctaatta tattgttcga gaacaggtat
     6721 tggaaaatat gactgcatat attacagata agtacccgga agataaaaaa ctacttgaat
     6781 atgtacagca aattcaagca atcgaagaat aaataaaagg aatgaagaaa atatggcatt
     6841 tgaaagctta actgaacgtc tccagcaggc gatgggaaaa ataagaaaaa aaggaaaagt
     6901 atcagaagct gacgtaaaag aaatgatgcg ggaaatccgc cttgccttac tagaagccga
     6961 tgttaacttg caagtagtca aagattttac taagcgcgta cgcgaacgtg cggtaggggc
     7021 agaagtgctg gaaagtttat cacctgcaca acaaatcgta aaaatcgttg atgaagaatt
     7081 gacgatcaca cttggttctg aaacagcaga gttaaataaa tcaccaaaaa taccaactgt
     7141 tatcatgatg gctggtcttc aaggggcagg taaaacgacc tttactggta aattagccaa
     7201 ttatttaaag aaaaatgaaa atgctcgtcc gctcctaatc gcaggggacg tatatcgacc
     7261 agctgcgatc gaccaattga aagtattagg tcaacagcta gacgttcctg tatttgatat
     7321 gggaactgaa gtcagcccgg tagagatcgt ccgtcaaggg atggagttag caaaagaaaa
     7381 gaaaaacgac tatgtattga tcgatacggc aggacgtctg catatcgatg aaacattgat
     7441 ggatgagttg aaacaaatca aagaattgac gcaaccaaat gagattttgc ttgttgtcga
     7501 tgccatgact ggtcaagatg ccgtcaacgt agctgatagc ttcaaccagc aattaggtat
     7561 tacaggggtc gtcatcacta aacttgatgg ggatactcga ggcggggcag cactatcgat
     7621 ccgctcagtg acaggcgcac caatcaaatt catcggttct ggtgaaaaat tgactgactt
     7681 agaagtattc cacccagatc gtatggctag ccgtatcctt ggtatggggg atatgctgac
     7741 attgattgaa aaagcgcagc aagactacga cgagaaaaaa gcggaagaac ttgcacagaa
     7801 aatgcgggaa aattcattcg atttcaatga ctttattgaa cagcttgatc aagtgatggg
     7861 tatgggccca ctggaagatc taatcaaaat gatcccagga atgaatcaag tccctggaat
     7921 cgaaaacgtc aaggtagatc caaaagatgt tgaacgcaaa aaagcgatgg tttattctat
     7981 gacgccagca gaaagagcaa atcctgatct gttgaaccca agccgtcgtc gccgaattgc
     8041 tgccggttct ggaaacagtg ttgttgaagt gaatcgaatg atcaaacaat tcaaggaatc
     8101 aagaaaaatg atgcagcaaa tgtccaaagg ggatatgaac atcccaggta tggaccaaat
     8161 gtttggttca ggcgtcaaag gaaaactagg caaaatggcg atgaatcgaa tgatcaagaa
     8221 aaataagaag aaaaagaaaa aacggaaata agatgaaaaa aacagcttcg gtacagactg
     8281 aaaatgtcta ctgccggagc ttttctatca gcaattttta ttctttacta atacaattac
     8341 tctaaacgaa gaggtggcac agatgagatg tccgtggagt gaacaaacag aaagtatgcg
     8401 gcaatatcat gatactgtct ggggtgttcc tgaatatgat gaccaaaagc tgttccgaaa
     8461 attgatgctt gatattaatc aagccggatt gagctggcag acgattttaa ataaaagtga
     8521 agcattcgat gaagcatatg atcgattcga tatcaaaaaa gtcgcaaatt atggagaaac
     8581 aaaaatcagt gaactaatgg aaaacaaagg aattattcgt aaccgaagaa aaatcgaagc
     8641 agccgtccaa aacgccaaag cagtcttgaa aattcaagaa gaaatcggca gtttttcaga
     8701 gtatctttgg tcgttttcta atggaaaaat cgttgattct tcttatcatt cacaagaaga
     8761 agtcccaaca acaagtcctc tttcagatga attagcaaaa gatctaaaga aaagaggatt
     8821 taaatttatc ggaagcacaa caatctatgc ttttttagaa gctgtcggga tcatcaatga
     8881 tcaccttgac agttgtttcc gcaaagaaga ggtcgtacgt atcggcagaa agggcgaata
     8941 aagcatgcga aaaatcatat tttatggtgc tataagttta gatggctatt tggcaacgaa
     9001 aacggatgat cttcaatggc tttttgacac tccaacaggt aagcaaacta cttatgaagc
     9061 tttttatgaa acaatcgata cgaccatcat gggcagaaaa acttatcagg aagcaaaaaa
     9121 atatttggag accgaagcaa tttatccaga gaaaaagaat ttcgtttttt ccacccacgc
     9181 acaattacaa ttgccagatg ctgaggtcgt aaacgaagat cctgttgctt ttctaaaaaa
     9241 tctacaaatg acagaaggaa aagatatctg ggttgtgggt ggcggtcgat tgatcaaacc
     9301 gctattggaa catcaaatga ttgatgaatg gtatatccag attacccctg tattattagg
     9361 ggacgggatt cctttattcc aaaaaggaac atatgaagcg cgttttgaaa tgctagacac
     9421 gacacgattc ggtgaattta tcgaattgca ttatgtacga aagtagaagt aaataaaata
     9481 aaaaaggcaa ctatatgcag aaatgtcctg tacatagttg ccttttttat taccgttatg
     9541 ctttttactc tgttaatttg ttagaaaaat ctagatacgc atctaaaatc gtttggaaga
     9601 aacttaatgt tccttcaacg atattaccat tttcatcaat tagattcgca acgtttccga
     9661 tatatgcttc cggttgctgc aaggttggca cattcaaaaa gacaagtgat tggcgtagat
     9721 ggtggtttgc gccaaagccg ccgatcccac ctggtgatgc agtaacgact aaacctggtt
     9781 tatgatccca aacactgtgt ccataaggtc ttgagcctac atctaaagca tttttcaata
     9841 ctgctggcac agaacggttg tattcaggag taacgaaaac gacaccgtct agtcccctca
     9901 cttcctcacg aaaacgagtc cattcagcag gagcctgttc tggagtttcg agatcctcat
     9961 tgtaaaatgg cagatcatcg atttttacaa atacactttc gtacccttca ggaagcaatt
    10021 tcgcaaaagc ttctgctact tttttgttgt aagaatcttt tcttaaacta ccaataaaaa
    10081 atccaatttt ttttgtcata cgatttttcc tccttcaaac tatctataat aatcttacaa
    10141 aagataagta actggagcaa ataaaaacac ttactgacaa aagctacata gaagatagat
    10201 tacttatttt taacaaaaaa gaagtctaaa atcttctatt ttctaaaaaa aatcgaattt
    10261 aatcaagctg taaagaaaaa aagctttaca gggtaaaaaa aaactgttac actacatctt
    10321 gtgaataaaa caatggaggt ggaaataaat aatggcagtt aaaatccgtt taaaacgtat
    10381 gggttctaaa aagagtccgt tttaccgtat cgtcgtagct gattcacgtt ctcctcgtga
    10441 tggacgtttc atcgaaactg taggtactta caatcctttg aaagaccctg cagaagtagt
    10501 tttaaaagaa gacttagttc ttgactggtt atcaaaaggt gcacaacctt ctgacactgt
    10561 acgtaacatc ctttcaaaag aaggcgttat gcaaaaacac cacgaagcta aattctcaaa
    10621 gaaataaggt gaccggatat gaaagattta agcgacttag tcttaacaat cgttcgtccg
    10681 ctagtcactt atcctgatca agtcaagtta gacgtcgtag agtcgaaaga cttttacgag
    10741 tacaatctaa cagtggctcc tgaagatatt ggccgtatca ttggaaaaca agggcgtgtt
    10801 gctaaagcaa tccgcacaat cgtttacggt gttcggatca atgcaccgaa aaaagtgcgt
    10861 ttgaatatca tcgatgataa ggaaaagtaa ataaacgtgc tgtactcgaa ttttcgagtg
    10921 cagctttttt gtttttttta ttaattgaag aaatgattat ttggcaaaac aagcaagaaa
    10981 gcgtatcctt ataggaggag ggagtgaaca gccaatgaga aagaaaatgt tatttattcc
    11041 atttgctcta ttgttaagcg cttgtagttc ggttactcaa gaatcagaca atacacatct
    11101 atcaagcagt atatctgaaa ctgttaccag tccagaaaaa agtagtacca aaacggcttc
    11161 caccactgaa caaacaatgg ctacctcaaa taatgagaaa aagactgctt tagaccagtt
    11221 gaaagaacaa cagccaaatg ttcccatgcc tctagatgta ccagtctctt caggctatct
    11281 caatatcgca gcgacacata caaagcaagg ttattctatt ttatattaca gaacagatcg
    11341 tcccctcggg ttgaatgctg acgaattgaa tcaagaaacg cctattgcaa cctatctata
    11401 tcaatatggg tttgcttcta gtcaagaaac aatccaagtt ttgcagccat ttgagatcga
    11461 cacaaatggt cagcaagtgg acttaggttc tcgaatcaca gggtatcagc aaggtgcagc
    11521 aggttcaagt tttcttgaat ggcaagaagg aaactggcgt attcgaatcc gtgggaataa
    11581 tatagaaggc caagatccct tactattagc taaagaaatc gtagcttatt tggaagaaaa
    11641 cagtctacca gcacctgaac aatttggtaa aattactgtt gatatgggtg atacgacgaa
    11701 tcgagccgta gaagtaagct ggcaagaacc gaaaaatgcc tatacgatca ctcatcaaga
    11761 tcctatgtct gcgttgaaga tggcagtttc tatgaagagg ttgtgaaatc agcgcgctga
    11821 tctgagccca tcatttaact ccaagtcaca ggaagtaatc aaaaatagca cgccgcattt
    11881 ttgcataatt ttggcttaat accttaaaag aaaactagaa ttgcctcttc acacatgcta
    11941 aaataaaaca gacaaaaaag aaatggggat ataagttgac tgaatacttg aatgtaggaa
    12001 aaatcgtaaa cacacaaggt atcaaaggcg aagtccgtgt tatctcaaca acggatttcc
    12061 cagaagaacg ttacaaaaaa ggaacggtgc taacactatt ccaagacgga aaagcacctg
    12121 tggaattaac agtcaaaagt catcgcaaac ataaaaattt tgacttattg agttttgaag
    12181 gacatccttc tatcaatgat gtggaaaaat atcgggatgg catattaaaa gtgtcaaaag
    12241 acaactctgt agatctagct gaaaatgaat tctattacca tgagatcatc ggtctgcatg
    12301 tattagaaga tggaactgag ttaggtaaag taaaagaaat cttgtcacca ggtgccaatg
    12361 atgtttgggt cgttcaacgt ccgaaaaaag ctgatatatt gcttccatat atcgcttctg
    12421 tcgtcaaaga agtcgattta gaagctggag tggtccgtgt agaaattcca gaaggactga
    12481 ttgacgatga agattgatgt attgacttta tttccaagaa tgtttgaagg tcctatgggg
    12541 gaatccatca tcggtaaggc tgtagataaa ggattattag atatcaatat ctctaacttc
    12601 cgcgattatt cagataacaa acatcagaca gtagatgatt atccatacgg cggtggagca
    12661 ggtatgctgc tcaaagtcca gcccatctat gataacatcc aagccattga aaaagctgca
    12721 ccggatgtca aaaaaagagt aattcttctt gacccagctg gtaaacgatt cgatcagaaa
    12781 atggcagaag aattttcaca agaagaacac ttagtattca tttgtggtca ttatgaggga
    12841 tatgatgaac gtatccgcac attagtgaca gatgaagttt ccttaggaga ttatgtcctt
    12901 actgggggag aattaggtgc gatggtcatg atcgatgcaa cagttcggct tttgccagat
    12961 gtgttaggga atcagacttc agctcaaacg gattcttatt caaccggttt attggaacat
    13021 ccccaatata ctcgcccggc agaatataaa gggatgaaag taccagaagt attgacaaat
    13081 ggaaatcata aattgattga agaatggcaa ttaaaagaat cactaagaag gacctatcaa
    13141 cgtcggcctg atcttttgga aacacttgaa tggacacctc aaatgacgaa atttttagaa
    13201 gaaatcaaga aagaagaaca agaaaaaaca gcagatgaag ccaagtgact ttttctaaaa
    13261 tcttctttac agtaggttga taatatgtta gtattttatg gtgagtgcaa aagcacttaa
    13321 ctattacgat attccgctgt gagaaaagct cataagaata tttggaataa ggagaagaaa
    13381 accatgaatc cattaatcga agaattaaca aaagaacaat tacgttctga catcccagcg
    13441 ttccgccctg gtgacactgt gcgtgttcac gcaaaagttg ttgaaggtac tcgtgaacgt
    13501 atccagttat ttgaaggtgt tgttatcaaa cgccgcggtg ctggaatcag cgaaacttat
    13561 acagtacgta aagtttctaa cggagtgggg gttgaacgta cattcccatt gcatacacca
    13621 cgtgttgcaa aaatcgaagt agttcgctac ggtaaagtac gtcgtgcaaa attgtactac
    13681 ctacgtgcat tacacggaaa agctgctcgt atcaaagaaa tccgtcgtta atcattgtta
    13741 tcagactgag aaagaaccct tggtattcaa gggttctttt ttcttagcat gtaatttgaa
    13801 caccctgatc cagttttttc tttaattatt ggtaggggag aataatggat tagaataaat
    13861 ctacctataa gtagtagtaa aaaaaagagc actatgataa tatctttctt aagagtaacc
    13921 gctttaataa aaaatccaga atattctaac ataaatggag tattcttaga atcaaaagga
    13981 gaaagaagat ggtttatact tatacggagc aagaaaagat gaaagtttta gacacattca
    14041 tgaatgatgg aaaagtaaaa caagtaccaa gttcaacaaa aaagaaatac atcctattga
    14101 aagaagtctt gaaacgcttt gaccatggag tgacttatac agaaacagag gttaattcga
    14161 tactgctgaa cgtattttct agtggcgatt atgtcgaaca aagacgttat ctaatcacat
    14221 ttggcttttt caaaagaagt tcagacggtc gggcatatca gatgatggga attgaaaatt
    14281 agaagtttta atttatactc attgaaaaaa gttcaagtat gcgatattgg ttctacaccg
    14341 catacttgaa ctttttttgt catctgtatc tataatacaa agccaagaac gttattcaaa
    14401 atatggatag ctatgataaa agctaatgag cgattcgcag ttaaataagc aatcgtcagt
    14461 aaaatcgttc caggagcgta catgaaaaaa tcaattggat gcgttacatg aataaagata
    14521 attctctttt atttgtacga ataaatacca aaacgtacct cctaaaatta gattttggac
    14581 agatgccctt taaaaagata acaagatttc gttcttgaaa caatatatat gcttttattc
    14641 caatgaggtt gtgaaaacag tattttgtcc tgagcgttag aaaattgaaa atggggaata
    14701 gcaactcgta ctttaccatt ttttaagtga atacaagatc agctaattga acaccgttta
    14761 ttcgtgatga gggacacaac actgcagtcg acacatttct cttttatgtt caaagataac
    14821 aaatatttaa aacaaagaaa aatagtagat aatcccgtcc taactctttt attcttccct
    14881 catagcttcc aaatttaagc aatacttcaa ttaatcaagt tttatctttt tttttcaata
    14941 atcgtaaaaa aagagtattt tttatt
//
LOCUS       DACECY010000063        14649 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.067, whole genome shotgun sequence.
ACCESSION   DACECY010000063 DACECY010000000
VERSION     DACECY010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.067"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            332..946
                     /gene="gmk"
                     /locus_tag="IWO38_002158"
     CDS             332..946
                     /gene="gmk"
                     /locus_tag="IWO38_002158"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ0314236.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            951..1256
                     /locus_tag="IWO38_002159"
     CDS             951..1256
                     /locus_tag="IWO38_002159"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ0314237.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            1509..3917
                     /gene="priA"
                     /locus_tag="IWO38_002160"
     CDS             1509..3917
                     /gene="priA"
                     /locus_tag="IWO38_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ0314238.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            3932..4423
                     /gene="def"
                     /locus_tag="IWO38_002161"
     CDS             3932..4423
                     /gene="def"
                     /locus_tag="IWO38_002161"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ0314239.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            4416..5354
                     /locus_tag="IWO38_002162"
     CDS             4416..5354
                     /locus_tag="IWO38_002162"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ0314240.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            5354..6712
                     /gene="rsmB"
                     /locus_tag="IWO38_002163"
     CDS             5354..6712
                     /gene="rsmB"
                     /locus_tag="IWO38_002163"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ0314241.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            6725..7465
                     /locus_tag="IWO38_002164"
     CDS             6725..7465
                     /locus_tag="IWO38_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ0314242.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            7462..9531
                     /gene="pknB"
                     /locus_tag="IWO38_002165"
     CDS             7462..9531
                     /gene="pknB"
                     /locus_tag="IWO38_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ0314243.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            9696..10595
                     /gene="rsgA"
                     /locus_tag="IWO38_002166"
     CDS             9696..10595
                     /gene="rsgA"
                     /locus_tag="IWO38_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ0314244.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            10608..11258
                     /locus_tag="IWO38_002167"
     CDS             10608..11258
                     /locus_tag="IWO38_002167"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ0314245.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            11259..11897
                     /locus_tag="IWO38_002168"
     CDS             11259..11897
                     /locus_tag="IWO38_002168"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ0314246.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            12052..12906
                     /gene="mreC"
                     /locus_tag="IWO38_002169"
     CDS             12052..12906
                     /gene="mreC"
                     /locus_tag="IWO38_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ0314247.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            12910..13419
                     /gene="mreD"
                     /locus_tag="IWO38_002170"
     CDS             12910..13419
                     /gene="mreD"
                     /locus_tag="IWO38_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ0314248.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            13674..>14649
                     /locus_tag="IWO38_002171"
     CDS             13674..>14649
                     /locus_tag="IWO38_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314249.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTT"
BASE COUNT         4923 a         2719 c         3185 g         3822 t
ORIGIN      
        1 tattaagcca aatttatgca aaaatgtgag aaactatttt cacaaaattt ttcttcatac
       61 tcgaaactaa tcggcttctt cacgacctct tatacacggc gttcaaaagc ttgcatctgc
      121 gataataacc ccaaccaaac gaaaaacatg agaagctgtt tatgtttggc tgttcttatt
      181 acttgatgca ggacagcttt tttacgacct cttggtacag ggtagctttt ttaccaattt
      241 ctagctataa ttaaagataa ccttgaaagt tgcagaaaaa aagtgcaaaa taaacgataa
      301 gtacaaaaag actagttgaa aggacgtcgt catgtcagag cgtggattat taatcgtgtt
      361 gtctggtcca tccggtgtag gaaaaggcac agtacgaaaa gcaatttttg agagtgagga
      421 taatgatttt cagtattcga tctcaatgac tacccgtaaa atgcgtgaag gggaggtcga
      481 aggcgttgat tatttcttcc gaagcaaaga agagtttgaa tcaatgatcg aagctggaga
      541 gatgctggaa tacgcagaat atgtcgggaa ttattatgga actcctttgt catatatcaa
      601 caagacattg gatgaaggaa aagatgtttt cttggagatt gaagtccaag gagcaaaaca
      661 agtcaaagaa aaagtgcccg atggtgtatt catctttttg actccgccag atttagcgga
      721 attaaaatca cggatcgtag gccgaggaac agatgcagat gaagtaatcg aagaacgaat
      781 gaaagttgca aaagaagaaa tcgaaatgat ggctctttac gactatgctg ttgtaaatga
      841 tgaagtgccg ctagcagttg aacgaatcaa aaacattatt gccagtgaac attttcgagt
      901 agatcgcgta atcggtaaat atattaaaat gttaaaggag atgtagtctt atgatgttaa
      961 aaccttcaat tgactcacta ttagatcgtg taaattcaaa atattctcta gtgattttag
     1021 caagtaaacg cgcgcatgaa ttggatgcaa gtgcacagcc tactctagat tcttttgatt
     1081 cagtaaaaag cgtcggtcaa gctttagaag aaatcgatgc aggaaatgta gtcaatgatc
     1141 cacatcctga attaaaacgt gaacgtttga agatggaaga agaggaacgt caagcacaaa
     1201 aagagcgtga acaacacgaa ttggaatcac gtatccgtga agagcaaaaa atgtaataaa
     1261 gaaaaataga gcgaacatct aaaaagaaaa gcaaacccag ctgtttcctt ttcaaacatt
     1321 cgccacttat tgtctggtag aaaaggctgt gtctgaactt tttgacacag ttcttttttt
     1381 tcttatctgc tgttaatttt ttgttaggat ttggcagaag ataagacaaa tccatcgatc
     1441 tctagtaaaa taggcaagga actttttgtc gcttgataag aaaagacaaa aaaaggagga
     1501 aacgaaccat gacattaaca gctgaagtca ttgtggacgt acctactatg cagaccgatc
     1561 agccattcac ttatttggtt ccctctgaaa tggaaacagc tttgcaagtc gggatgcgag
     1621 tagaagtccc ttttggtaat ggcaatcgtc atgtccaagg atttgttatg gctatccaaa
     1681 aatcagaaga atcagctaat ccatctttga aggcagttat tcgtttgcta gaccttgctc
     1741 cggtggtcaa tgaagaatta ctttcattag ctgattatat gaaaaaaatt acttacgcat
     1801 tcaaaatcac ttgtctacaa acgatgcttc cgagtgtgat gaaagcagaa tatgataaat
     1861 tgatctatcc tttggcagac acaccagaag ttcaggtatt gtttgatcag cgagaagtga
     1921 tttcctggaa ggaagcagaa gaagaaggca gcctttctca attgattcgc tggcgccaag
     1981 agcaattagt agatatcaaa tatgaagtcc atacacgaaa taaagtaaaa acgattcgtt
     2041 tggtccgttc tttgttaact gaaaagcaaa tcgaagaaga atgggcaaaa ttacggcaaa
     2101 atgccaagaa acaaaaagaa cttcttttgt gtttatctga actgagccaa gaagaaccaa
     2161 ttgcttattt caaaaataaa ggaatcagta ctgctgtctt gaatcaagga aaagaaaagg
     2221 gctggctgga gtttatagaa tcagaacgtt atcgcgaccc ttataaagac cgagtatttg
     2281 accaaacgac ggcattggaa ctgaatgcag aacaaaaaaa tgctgtcgaa caaatcatca
     2341 cagcaggtca gcagcaaaaa gatcaggtct ttttgctgga gggaatcact ggcagcggga
     2401 aaacagaagt atatcttcag gcaatcgctg atgttttgtc agaaaataaa acggctatta
     2461 tgttagtgcc tgaaatcgca ttgactccgc aaatggtcga gcgattcaaa ggacgcttcg
     2521 gagaatcagt tgcggtctta catagcggac tttcccaagg tgaaaaatat gatgaatggc
     2581 gcaagattga gcgggaagaa gcgcaagtag tagtaggcgc acgatctgct atttttgccc
     2641 cgctgaaaaa tatcgggctg atcattatcg atgaagaaca tgaatcaagt tacaaacaag
     2701 atgagacacc gcgttatcat gcgagagatc tagcaatttg gcgcagtaaa tatcatcatt
     2761 gcccaatagt tctaggaagc gcgacgccct ctttagaatc aagagcacga gctcaaaaag
     2821 gcgtttatca gttattgcag ttaaaccacc gagccaaagc agctgcacag cttccagcta
     2881 ttgagatcgt agatatgcgt gaagagtttc agaatcatcg gacctctact ttttctgcta
     2941 atttgcagga aaaaatacaa aatcgattag acaaaaaaga acaaactgtc ctactgctca
     3001 atcgtcgagg atattcgtct tttgtgatgt gtagggactg tggatttgtt cttccatgtc
     3061 ctaattgcga tatctctctt acactgcata tggatacacg ttcaatgcgc tgccattatt
     3121 gcggacatga agaacctatt cccaatcgct gtcctaattg tggcggcaat aaaatccgct
     3181 actatggaac aggtactcaa aaagtagaag aggaactccg agaactttat ccgcaagcta
     3241 ggattttgcg tatggatgta gataccactc gaagaaaagg ggcacacgaa cagattttac
     3301 aaaaattcgg tgcaaaagaa gcagatatat tattagggac acaaatgatt gctaaaggtt
     3361 tggactttcc agaagtgaca ttagttgggg tgttgaatgc tgatacctcc ctgaatttac
     3421 cggattttcg ttctagtgag catacattcc aattgcttac ccaagtttca ggacgagcag
     3481 ggcgagcgga aaaagcaggc gaagtcgtga tccaaacatt caatcctcaa cattacgcca
     3541 tagaattagc taaaaaacaa aattatgagc agttttatca gcaggaaatg catgtacggc
     3601 atcgtggagg ctatcctcct tattacttta ctgtgaaaat aaccgccagc catcctgaag
     3661 aacaagtagc ggcaaagaaa atctttcaga ttgccaatca gctaaaagaa gtgttaagcc
     3721 ctcaaagctt gctattaggg ccaacgccca gcatgatttt gagagtgaaa aaccgatatt
     3781 attatcaact aatcatcaaa tataaacatg aaccgaatct tcctcatgtt ttggatgaaa
     3841 ttttgaatgg aagccagaaa gaacagcgac aaggtctttt tgtggcaatt gacaacgaac
     3901 cattaaactt tatttaagaa agggccaaat tatgcgctat cctatattga tccaccctaa
     3961 tgataaatta aaaagaactg ctcagccaat cgatgtaatc acggacgaga caatcgcttt
     4021 attagacaat ttatatgaga caatgattgc gaatgacgga atagggatcg ctgctcctca
     4081 agtaggacaa aacaagagaa ttgctgtgat cgaagtagat gaaggcgaga aatttgaact
     4141 gattaaccca gaaatcatcg aagcaaaagg tgaaagcctt gacgtagaag gttgtctcag
     4201 tattccgcat gtctatggaa cggtcaagcg agcagatgaa gtgaccgtgc gttattatga
     4261 tcgagacgga gaagagatag aagtgacggc ttttggctat ttagcccgtg ccttccagca
     4321 tgaaatcgat cacttagacg gtatcttgtt cattgagaaa atgatacagc agatcccgga
     4381 agaagaactt gaagaatata tggaggaaca tcttgatgac taagctagta tttatgggca
     4441 cacctgcttt ttctgtgccg attcttgaag ggctgataga atcaggttac gagatccagg
     4501 cagtggtaac acagcccgac cgtccagttg ggcggaaacg agtaattacg ccgacaccgg
     4561 taaaagaagc ggctctgaag cacggtatcc gtgtgctgca gccagagaaa atctccggtt
     4621 cgccagaaat ggaagaaatc attgaattgg tgcctgatgt gatcgtcact gcggcatttg
     4681 gccaattctt gcctgaaaag ttattgcaag taccaaaatt aggggcaatc aatgtccatg
     4741 cttctttact tcctaaatat cggggaggtg cgcctgttca ttatgccatc atgaatgggg
     4801 agaaagaaac tggtgtaacg atcatggaaa tgatcaaaaa aatggatgca ggcggaatct
     4861 atgcgcaaga aagcatacca atcactaaac aagatgatgt agggacgatg tttgaaaagc
     4921 ttagcctttt aggaagaaag ctattgctag aaacattgcc caatattttg gatggacaaa
     4981 aaccagtacc tcaagacgag tcagaagcaa ctttttcgcc aaacatcaca cgggaacaag
     5041 aagcaatcga ttggcataaa actgcagaag aaattgacaa ccaagtacgc ggaatgcgtc
     5101 cttggccaat tgcattcacg acctatgagg gcatacgctg gaaattatta tcggttgaag
     5161 tattggcaga aactacagac aaagcacctg gtacgatcat ccggaaagac aaaaaaaatc
     5221 tatggattgc ttgcggtaaa cagaccgtct tagccatcaa tgaattacag ccagctggta
     5281 aaggaaaaca ggccgtacat gatttcctta acggaagcgg tcagcaagtt cagattggtc
     5341 agcaggtgaa ataatgaaaa aaatccctaa aaaaatgaaa aaatcagtac gatatatggc
     5401 attactgaca ttagagcgag tgaacaaggg tggcgcctac tcgaatcttt tgttgaatga
     5461 gatgatcaaa caaagcgagc taaatcaaaa agatgtgggt ttatttacag aattgatcta
     5521 tggtacgatt agccatcagc gactattgga attttacgtt gcaccattga tcgctaaagc
     5581 aaaaaaagtg gatgactggg tcaagacgct tctttatctt tctgtatacc aattagaatt
     5641 tttagataaa gtccctgcac atgctatttt aaacgaggcg gtcgaaatcg caaaagtaaa
     5701 aggaaatcct gggacaggga aatttgtaaa tggtgtcttg aggaattatc agcgtcaagg
     5761 agcaccagat ctgcaaggca ttgctgatcc tatcgaaaga ctttctgtca aaatcagcct
     5821 gcctctatgg atgacgaaac gactggtgga tcaaatcgga tacgaagaaa ctgaaaagct
     5881 cggtttatcg ctatatcagc ctagccatgc tagtgcccga gtagatacac gccgactttc
     5941 cagagatcag gccatcactg ttttgcaaga agaagagatc acagctgaaa aaagccaagt
     6001 cagtccatac ggtatagtag cgaaaaaagg gcatttagct tctagctcat tgttccacga
     6061 tggcgtcatg acgatccaag atgaaagttc gatgcttgta gcgccgtcta tgcaaatcga
     6121 gcctgaacat catgtcttag atgcctgtgc agcacccgga ggtaagacga cgcatattgc
     6181 ttctttttta gatccgtcat gcggtggaga agtggtcgct ttagatgtac acgaacacaa
     6241 agtcaaactc attgaagata atgcagcacg tctaggtgtc tcttctgctg tctacgctca
     6301 aaagatggat gctagagaag tcagtaaaca gttcgaagca gagcagtttg atcggatttt
     6361 agtagacgcg ccttgttcag gtcttgggct gcttcggcga aaaccagata ttcgttataa
     6421 aaaacagccg gatgaattag cgaatttacc aaaaatccaa ttggaaattt tagaaagctg
     6481 cgcaccaact ttaaagtctt ccggtattct gacttatagc acttgtacga tcttaaaaga
     6541 agaaaaccaa gacgttgttt ctgctttctt ggaaagacac cctgattttg agaggattga
     6601 tgttttgacc aatcctatgc tacaatcatc aatcgaagag aaaatgttga ctttgtatcc
     6661 gcatcagttt tacacagatg gatttttcat ttgttgtttg cgcaaaaagt aatgaggtga
     6721 aaaaatgcag attgaatatc aatcagatgt aggaagacga cgtaacacaa atcaagacta
     6781 tgccagtgta tttaccagcc gttcaggtct caagcttgct gtattagcag acgggatggg
     6841 tggtcatcgt gccggagaca ttgccagtca gatggcggta acgaatctag gcgaagcttg
     6901 ggaaaaacaa gaagctgttg acgatgaaaa aatcgcccaa tggttcattc aatcgattca
     6961 agaagaaaat atgctgattt atcagcgtgg acaagaacag ccggaatata acggtatggg
     7021 aacaacaatc gtagcggccg ctctgtcaga tgactggttc acgattgcca atgttgggga
     7081 tagcagagct tatttgattc gtgaagataa aatcatgcaa ttgacggagg atcattcttt
     7141 agtcaatgaa ctagtcaaat caggagaaat cagcgaagaa atggcagtga accatccaag
     7201 gaaaaatatt ttaacacgtt ctgtcggcat gcctggaaca gtcgaagtag atgtttctac
     7261 tcatctttgg caactaaaag atcgactgct gttatgttca gatggcttga cgaacatgct
     7321 gtctgaagaa gaaataggaa cgatcatcaa tcaagaaggc ccattgtctc ataaagtaac
     7381 tgagctgatt gatcgtgcca atgaagcagg cggagcggat aatatcaccg ttcttcttat
     7441 cgaatataag gaggacagtg tatgatcgag ttgggcaaaa aattaaatgg ccgttatcaa
     7501 atcactggaa atatcggtag cggtggtatg gcaaatgtat ttttggcgca cgatttaatc
     7561 ttggatcgtg atgtagcagt taaagtctta cgatttgact ttcaaaatga ccagactgcg
     7621 attcgacgat ttcagagaga agctttagca gcaacagaac tcgttcatcc aaacattgtt
     7681 agtgtttacg atgttggtga agaagacaac atgcagtatc tggtgatgga atacgtcaaa
     7741 ggaatggact tgaaacgata tatccaaaca cattatcctg ttccttatga aacagcagta
     7801 aatattatgc agcagatcct ttctgctatt tctttagctc ataaccatca aatcatccac
     7861 cgagatttga aaccccaaaa cgtactgatc gataatgaag gtgttgtaaa aatcacggat
     7921 ttcgggattg ctatcgcttt atctgaaaca tcgatcacgc agacgaatac gatgctggga
     7981 tctgttcatt atctgtcccc tgagcaggcc agaggaagta tggcaacgaa gcagtcagat
     8041 atttatgctt tagggatcat cctttatgag atgttgacgg gtagcgtacc gtttgatggt
     8101 gaatctgctg taacaattgc tttaaaacat tttcaagatg atcttccatc tataaaagca
     8161 cttgatccaa atgtgccaca agcactggaa aacgtgattt tacgggcaac ggctaaagaa
     8221 cctgcagacc gttataagtc agccgaggaa atgtctgatg atctttctac agtattatca
     8281 ccagctcgtg caaacgaaga aaaatggcag cctcatgtga tggataatga aacaaaagtt
     8341 atcacacccc ttacggaaga tgcaccaatg cctgattcat tcaaatctat gcctctgcca
     8401 aaagacaagc cagaaaacga aaccttgcca gaggaggaag aagttctgcc tgatcctaaa
     8461 aagaaaggac agaaaaaatg gtggctgatc ctattagcta ttcttgctgt tttaggaatc
     8521 ggaacagctg tattctttgc ttctggcggg cgaggagaag ttgttgttcc tgatgtttct
     8581 gatctttctg aagcttctgc tagagaaaca ctcgcaaaag cgaaattaga agtagcaaca
     8641 aaaacagaag aaattgcaga tgacaaaatc gaagaaggta atgtagtgaa gacagacccg
     8701 gcagcgggaa cgacagtcaa gcaaaaacgc gaagtcactc tgtatattag tagcggcacg
     8761 aaaaaaatca aattagatga ttacagtggt aaaagttatg aagatgccga agcaagtttg
     8821 aaagaattag ggttttctgc tgacttgatt caaaaagaag aggaattcag ttcagaggta
     8881 gaaagcggaa tgatcatttc gcaatctcca tcagaagata cagaagtcga tcctaaaaca
     8941 gacacgatta ctttcacagt cagcaaagga ccagaaacag tgactttgag tagtcatgta
     9001 ggagaaaact acgaaactgt tcgtaattgg cttgttacac aaggattcaa tatcaataat
     9061 attagtgcta cttatgacta tagcgacact gtaccatcag gttcgatcat cagtcaggat
     9121 ccagcctcgg gtgaagttgt agcagaagaa acttacatca attttgtcgt aagccaagga
     9181 acagagccta aattgaatga tatctccggc tatacaaaaa gcgaagcaca aagttattta
     9241 gctagtgtcg gtgcagaata tatcgggcat gagacttccg aatattcaaa cacggtggat
     9301 aaagataaag tgattcgtac agtaccaggg gcaggaacga caattaccaa aggaatggtt
     9361 gtgaacgtaa tttactctaa aggaccagat ccagcgtctt ctagttctga aaaagactct
     9421 tcagacagtt ctagcagctc aagtagctcc agtagcacaa gcaactcttc aaaagaagaa
     9481 tcttccagct ccacaagttc atcagctagc acctcagaaa gttcaaaata agtgctgaat
     9541 aaaatatatc agaagggaat gcgacattga tttgttgcat tctctttttt gtgtttctat
     9601 ctcttttgat caattttcaa gccatatact ttgagaagtt tgcatggaat aacacctact
     9661 ttcctcttcg tatggtaaaa taaacaaaaa cggaggtggc acttatcaaa ggacaaatca
     9721 gaaaagcaat cagcggtttt tactatgtat acgcaaacgg agagacctat caaacaagag
     9781 gaagaggaaa tttccggaat cggaaaatca cgccgcttgt cggagatcag gtgatctttg
     9841 agagtgaaaa tcaaacagac ggctatcttc tggaaatctt gccgagaaaa aatcaattag
     9901 ttcgcccacc agtcacaaat gttgatcttg gaatcattgt cacaagtatc gtagagcctg
     9961 cattttctta taatctactt gaccgctttc tagttacctt ggaatacgag tcgatcgaac
    10021 cgattatttt tttgacaaag accgacttgt cagacgaccc agaaaaaatc gaagagatca
    10081 agcaaaccta tactaaaata ggctatccgg tgattgtacc caatcatacg ggtgatacga
    10141 cagaactgat ccgttacttc ccagaacggc tatcggtctt tatgggtcag tctggagcag
    10201 gaaaatcgac gctactgaat caaatatcac cggaactgaa tttagcaaca ggagagattt
    10261 ctgatgcact tggaagagga cggcatacaa cacgtcatgt ggaactgatt cctatctatg
    10321 gcgggttagt agcagataca ccaggattta gctcgattga ctttttgaca attgaagcag
    10381 gagaactgcc gagacaattt cctgaatttg tagaagcttc tgtccattgc aaatttagag
    10441 agtgcatgca tgctaaagaa cctggctgcg aagtaaaaca tagagtagaa acaggtggta
    10501 tcgcacagac acgttacgac aattatttgc aattcttaca agaaatcgaa aaaagaaaac
    10561 caatgtatgc aaaaaaagaa ggtaaacgaa aataggagga aacaagaatg aagatagcac
    10621 catccatttt gagtgcagat tttgccaatt tacaaagaga cattgagtta gtggaaaaag
    10681 gaggcgcaga ttacatccac gtagatgtaa tggatggcca atttgtccca aatatcacat
    10741 tagggccaaa tattgttcag gcaatccgtc ctgttactaa attaccgtta gacgtccatc
    10801 tgatgatcgt tgatccagaa aactacattc cagcatttgc aaaagctgga gcagatatca
    10861 ttactgtcca cgtcgaagca actcctcata ttcatcgggc acttcaaatg acaaaaagct
    10921 taggagttaa agctggagta gttatcaatc caggaacacc agtctccatg atcaaacatg
    10981 ttttgccttt ggctgatcaa gtactagtca tgactgtcaa tcctgggttc ggcggacaaa
    11041 gctttattga agaaacagta gaaaaaatta cggaactcgc tgatctacgt agagagaaca
    11101 attggactta tgcaatcgaa gtagatggcg gaatcgttcc agaaacagca aaaatctgta
    11161 aagatgcagg ggcggatgtc ttcgttgcag gatcatatat ctatggagcc gaaaatccag
    11221 tcggacaaat cgaacgatta aaagaggcat tagactaaat gaaaatttta ttagctgctg
    11281 gaggtaatcc gcaaaactgg ccgcagtctc tgctgtccaa atacgatgcc tatgtcggga
    11341 tagaccgtgg tgcgctgtac ctgcttcaag caggctaccc agtagatctg gcagtgggcg
    11401 attttgattc attgtcgaac gaagagcaga aggaagtatt tgcaaaagcc aaagagtata
    11461 cccgttcccc tgcagaaaaa gacgatacag atacacagtt aggtatctta aaaacatttg
    11521 aacgttaccc gcaagcggaa gtcactttga ttggagctac gggcggaaga ttggatcatc
    11581 ttttagccaa tctctggtta ggtatggaac ctcgcttcaa accctttgcc catcagctta
    11641 ttatcagaga ttgtcaaaat tatttgtctt attatttgcc tggagagcat atagtaagac
    11701 agataccaga tatgaagtat ttagcttatt gctgcttgac tcccgtctcg aatcttacat
    11761 taaaaggcag caagtatgta ttggaaaata aagaagtaga atatccgaca tcttatgcaa
    11821 gtaacgaatt tctttcagat accgcagaat tttctttttc agatggtatg attgcagtta
    11881 ttcaaagcaa agattgatcc ataaacaaat aaaaaaattt gtctttcagg ctaaaaatga
    11941 cagaatctat tatttgctaa aaaaaagctt aagaggagca acttaccttt tgtttcacgg
    12001 catttagtga taaaattgaa cacaagagtc taattaattc gaggtgtctt tgtgaagaag
    12061 ttcaatccta ataaaaatat tattattacc ttggttattg ttatcatcgt ggtgacgata
    12121 ctaagtgtga ccgctgcgca gcgagcgaat gaaggaaagc ccaatttctt ccaatccatc
    12181 gttaatgaca gtgttgcaat ggttgataaa acaatctctg ccccagtacg gtgggtacga
    12241 aatggagtag attctgtcca taatcttttt tcaacttatg ccgaaaatga gcggctaaaa
    12301 gaaaaaatcg atagctatga tgacgtttca cagcaaaaca agaattacga gagagaaatc
    12361 gaagcgttga aaaaagaact tgagttgaat cagacgctga ctagttacga aaaagtaacg
    12421 gcaaatgtca ttactcgttc gcctgacagt tggcaggaca tgctgatcgt agacaagggt
    12481 tctaaagacg gtatcgaagc aaatatggca gtcatgtcgc aaaaaggatt gattggccga
    12541 gtgatcgagg tcaatacggc ttcgtctaaa atcgaattac tgtcatcctc taatgaaagc
    12601 tccaatcatt ttccagtacg ggtatcttcg gctaatggcg aagcgtttgg tttgcttaaa
    12661 aactatgatg aaaagctcca tgccttagtg gtgacccaat taactggtga tacggatatc
    12721 aaagaagggg atgttgtcca gacatccggt cttggaggga attctccagc taacttgccg
    12781 atcggtacgg ttattaaaac gaaaccagat agttatgggc tggatcggga agtttatgtg
    12841 aaaccttatg cagaaatgta tgacgtgtca gttgtgacga ttgtccaacg gctagcggag
    12901 gattagtcca tgttgaagaa agaaacaatg aagtactatc tgccaatcgt tttgttcttt
    12961 ttgatgttga tagatggtca tttaacaaga atgctagggg agtggtcgaa aggcacctat
    13021 atgtcaaatg cccactttct gatattggca ttattatgtt gcagtatggc gtttgaaaaa
    13081 cgttatttac tgattaccac gattgttctc ggggctatct atgacgctta ctatattggc
    13141 gttatcggta tctatgcagt agctctccct ttaattgtat ggttgatgta tgtaatgaaa
    13201 gacgttatcc atgtcaacat ctttactgaa tttttcagta tgatcatctt tgtcacgggt
    13261 tatgaattgt ttacgatggt ggtccagttg atttttaaat tagcagtagt aaataacacg
    13321 tattttatta caaggttttt aggacctaca ctgctgttga acatgattat atttgtatta
    13381 ttcatttttc cctttaagaa attattcagt gatgaataat ttcttttttt atttttaggt
    13441 cgaaagcgaa aaagaatcgt tgttatctta agaaatgcta gacttaacgc ggctttttca
    13501 tgatcatttt ctgaaacgaa cagttttgtc atttttaaaa gttgttttga aacatttttg
    13561 taacataact attatatcgt tgacacactg ctatggtaca atttttatat cgttaagaaa
    13621 gacttaaata tatttagata tttattaaat taagccagtc ggaggaatga agagtgaaaa
    13681 agagtttaat atcagcagta atggtatgtt caatgacctt gactgccgta gcatcaccga
    13741 tcgctgccgc ggcggacgat tttgattctc agatacaaca gcaagatcaa aagattgcag
    13801 acttgaaaaa tcaacaagca gatgcacaat cacaaatcga tgccttagaa agccaagttt
    13861 ctgaaatcaa cacacaagca caagacctat tagcaaaaca agatacattg cgtcaagaat
    13921 ctgcacaatt agtaaaagac attgcagatt tacaagaacg tattgaaaaa cgtgaagata
    13981 caatccaaaa acaagcacgc gaagcacaag taagcaacac aagctcaaac tacatcgatg
    14041 ctgtattgaa tgctgattca ttagcagatg ctattggacg cgttcaagca atgacaacga
    14101 tggtaaaagc aaacaacgac ttgatggaac aacaaaaaca agataaaaaa gctgtagaag
    14161 acaaaaaagc tgaaaacgat gctaaactaa aagaattagc tgaaaaccaa gctgctttgg
    14221 aatctcaaaa aggcgatctt ttatctaaac aagctgattt gaacgtactg aaaacttcat
    14281 tagctgctga acaagcaact gctgaagaca agaaagctga cttgaaccgt caaaaagcag
    14341 aagctgaagc agagcaagct cgtattcgtg aacaacaacg tctagcagaa caagctcgtc
    14401 aacaagctgc acaagaaaaa gctgaaaaag aagcacgcga acaagcagag gctgaagcac
    14461 aagctactca agcttcatct actgcacaaa gctcagcaac agaagaatca tcagctacac
    14521 aatcttcaat gactgaagaa tcatcatcag ctacacaatc ttcagcaaca gaagaatcaa
    14581 caacacctga atcttcaaca gaagaatcaa cagcgcctga atcttcagca acagaagaat
    14641 caacaacag
//
LOCUS       DACECY010000064        14448 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.068, whole genome shotgun sequence.
ACCESSION   DACECY010000064 DACECY010000000
VERSION     DACECY010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.068"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..203)
                     /locus_tag="IWO38_002172"
     CDS             complement(<1..203)
                     /locus_tag="IWO38_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314250.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRI"
     gene            complement(191..820)
                     /locus_tag="IWO38_002173"
     CDS             complement(191..820)
                     /locus_tag="IWO38_002173"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ0314251.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(827..1543)
                     /gene="pyrF"
                     /locus_tag="IWO38_002174"
     CDS             complement(827..1543)
                     /gene="pyrF"
                     /locus_tag="IWO38_002174"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ0314252.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKEIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(1540..2472)
                     /locus_tag="IWO38_002175"
     CDS             complement(1540..2472)
                     /locus_tag="IWO38_002175"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ0314253.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDPDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(2469..3245)
                     /locus_tag="IWO38_002176"
     CDS             complement(2469..3245)
                     /locus_tag="IWO38_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ0314254.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(3299..6484)
                     /gene="carB"
                     /locus_tag="IWO38_002177"
     CDS             complement(3299..6484)
                     /gene="carB"
                     /locus_tag="IWO38_002177"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ0314255.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(6489..7565)
                     /gene="carA"
                     /locus_tag="IWO38_002178"
     CDS             complement(6489..7565)
                     /gene="carA"
                     /locus_tag="IWO38_002178"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ0314256.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(7570..8850)
                     /locus_tag="IWO38_002179"
     CDS             complement(7570..8850)
                     /locus_tag="IWO38_002179"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ0314257.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(8869..9795)
                     /locus_tag="IWO38_002180"
     CDS             complement(8869..9795)
                     /locus_tag="IWO38_002180"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ0314258.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(9808..11091)
                     /locus_tag="IWO38_002181"
     CDS             complement(9808..11091)
                     /locus_tag="IWO38_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ0314259.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(11105..11644)
                     /gene="pyrR"
                     /locus_tag="IWO38_002182"
     CDS             complement(11105..11644)
                     /gene="pyrR"
                     /locus_tag="IWO38_002182"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ0314260.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(11879..12790)
                     /locus_tag="IWO38_002183"
     CDS             complement(11879..12790)
                     /locus_tag="IWO38_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0314261.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKPAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(12787..13251)
                     /locus_tag="IWO38_002184"
     CDS             complement(12787..13251)
                     /locus_tag="IWO38_002184"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ0314262.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLVLAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICILIYIILDEKDQRKK"
     gene            complement(13256..13954)
                     /locus_tag="IWO38_002185"
     CDS             complement(13256..13954)
                     /locus_tag="IWO38_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0314263.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(13941..14327)
                     /locus_tag="IWO38_002186"
     CDS             complement(13941..14327)
                     /locus_tag="IWO38_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314264.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINDEKRSVLSHEKKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
BASE COUNT         3908 a         3312 c         2581 g         4647 t
ORIGIN      
        1 attattctct tctttctgct aaccagtctt agaaaaatga attttaaaaa aatcaaaaac
       61 tgatcacgat agaagtttct tttcatacct gatgaaaaga tgcttctcgc aatcagtttt
      121 tggatttttg ctattccaat tgatgattct ggagacacaa atcggatttc tctatcttca
      181 tcaagcgcgc ttaaatctgc caattttcag gatctttttt ccattctttt agcagagtta
      241 attcttgctc atcgatataa cgatcttcta aagctgcttc aatcaataca gaataattgg
      301 tcaacgtcat aagagggatt tcggcttttt cgaaatttgc ttttcctttt ggcaactcat
      361 acgtaaagat agccgcaaca ccaaggatat ccgcaccttc tctttttgct gcttcagctg
      421 cttccaagac acttccgcct gtagaaatca aatcctcgat cacaaccatc ttctgacctt
      481 caaatatacg tccttcgatt tggttacctt tcccatgatc tttcgcctta cttcgaatat
      541 agaccattgg caaatccagt atttccgcta cccatgcggc atgagggatt cctgctgttg
      601 cagttccagc aatcacttgt acttcagggt aagcttcttt gattttgctt gctaatcctt
      661 ttgcgatctc ttttcggact tttggatagc tcatcgtgat tcggttatca cagtaaattg
      721 ggcttttgat tccacttgcc catgtaaaag gattacttgg atttaaaaat actgcttcaa
      781 tttctaataa gtcttttgcg atactttgtt caacattcat tttaggttag ctccattctg
      841 tttgaatcgt ttgatatgct ttataaggat tagttgactg agtgattggt cgacctataa
      901 cgataaaagt tgagccgatc tcttttgcct gagaaggtgt gactactctt gtctgatctc
      961 ctgcgactgt tccttctggt cttatacccg gagttaagca aacaaattcg tctcctgcca
     1021 tttctttgat cgcttctact tcccaagcag aagaaaccac accatctaat cctgcttttt
     1081 tggcacagct agcataatgg atcacacttt tttctaaaga aacttcgatt agttgatcgg
     1141 catgcatttc ttcttcactt gtagaagtaa gctgagtgat agctagaagt agcggacgtt
     1201 ttccaccttc tggagtcccc tcttctaatc ctcgcattgc cgcctccatc atacgaatac
     1261 cgccagcggc atgaacacag gtgagatcaa caccaagctt tgccaatccc cgcattgctt
     1321 tttcgaccgt attcggaata tcgtgaagtt tcagatctaa aaatacatta tgaccagcct
     1381 cttttagata acgtacgatt tcaggtcctt cttggtagaa caattccatt ccgattttca
     1441 caaacaattt ttcttctttc gggaaaaatc gaagaaagtc ttctacctct tgcctagttg
     1501 aaaaatctaa tgcaataatt ggtcgttggt ctagttgatt catcgttcac gttcctctct
     1561 cacttctttt attaatcttt ccagcgattc gatgcctagt tcgctcattc gcacagggag
     1621 ctctttaatt agtttaggac agatatacgg atcggtgaaa ttcgcggtcc ctatagctac
     1681 agcacttgct ccagccataa acatctcaag aacatcatcc actgtctgca cgccgcccat
     1741 cccaatgatt ggaaggctgg atatacttgc tacttggtga atcaaacgaa tagcgacagg
     1801 cttgattgct ggtccggaga gtccacctgt ttgattggct aaaatcggtt ttcttgtctt
     1861 aaggtcaatc cgcataccaa gaagagtatt gatcatcgta aacccatctg cccctccagc
     1921 ttcgattgcc tgagcgattg gaacgatgtc tgttacattt ggggacaatt tgacatagac
     1981 aggaacagaa gaaactttct ttactgcttg tgttaattga aaagcaacat caggatctgt
     2041 tccaaaagca attccgccat gtttgacatt tgggcaagaa atattcagct cgattgcttt
     2101 cacatttggc gcttcgccaa ttttgctaca aacttctaca tagtcttctt cacaagcacc
     2161 agcaacattt gcgatgatgg gtaactcatt gtactcctct aattctggaa gaagtttatg
     2221 cataacaacg tctagaccag gattttgcaa accaattgca ttcaacatgc cagatggtgt
     2281 ttccgccact cttggtgtgg gattgccaaa gcgggcattt ggagtcgttg ccttgaccat
     2341 gatcgatcca agctgattca aatcatagta ttttccgtat tctttgccaa aaccgaaaca
     2401 gccgcttgcc ggcatgatag gattttttaa ttctagaccg gggagcttga ctgctaaagg
     2461 attctcattc atataattac ctctcctatt ttaaaaatcg gtccttcgtc acaaactttc
     2521 acactttttg ttcctgtttc atcttccggc gtatgacaaa cgcaagcata gcaagcccct
     2581 atcccacatg ccatgcggga ttctaatgat aactggacat tttgatgcga agaaaacagc
     2641 tgttccactg tcttcaataa cccgtttgaa ccacatgcaa aaacagctgt aggttccgtc
     2701 ttcgttccta acaataaatt accgacattt ccgtgcatac cgtatgttcc atcgtcagta
     2761 gcgattcttg tttctcctag acgattaaat tctttttcat aataaacgac ttcatgtgtg
     2821 gcaaaaccta aaaaatgcgt tggcttaatt cctttttcga ctaactgttt tgataactca
     2881 tatagaggag ggacaccgat tcctccaccg attataaata cttcgtctcc tgtatgaaga
     2941 ttggacaaat caaaaccatt gcctaaagga cccatgacat ctaaatggtc tccggcagaa
     3001 agagtagaga agactctagt cccctcacct tccacccgat aaatgatacg gcaagtattt
     3061 gtttcgtggt cgatatgatt caagctaatc ggtcggcgta atagcaagtc tgcacgcggc
     3121 acgagaatat gcaagaactg tcctggttgc tgcatttctt ttactagatc acctttcaat
     3181 gtcatttcaa aaatctttgg tgccagctgt ttttgctgaa cgatttccat catttcttgt
     3241 ttcatctgtc ccctcctgca acggtttttc gtctttttgt cttaacatgt gctcgccgtt
     3301 aaatagggtt agttgaaaaa gcacgggatt ccatcactcg aacgatcgca tctgctgtgt
     3361 ctaaagaagt aaacaatgga ataccatgtt caactgcttc tcttcggatg atgaaaccgt
     3421 ctttagaagc atttccccga tctttatcaa tcgtattgac aaccacttgt gttttaccat
     3481 ctcttattaa gtcgactaca tttttttctg ctgcttcctc agaaattttc gctactgttt
     3541 caacgaccag accatttttt tcgaaataag cagctgtatg tttcgttcct atcagactat
     3601 agccaatttc tgtaaaacgt ttagctaatt ctagtgcttc ttcttttgtt tcgtccgcaa
     3661 tcgtaaataa tacggctcca tattcaggca agtgtaatcc agaagcttcg aatgccttgt
     3721 acaaagcttt gtctaattgt ctgtcagatc ccattacttc tccagtcgat ttcatttcag
     3781 gccctaagta agtatcaact ttctgcaatt tcgtaaaaga aaagacaggt gctttaacat
     3841 gaacttcttt tgtttctgga taaagtccgt tttcataacc aagtgtcttt agtttctctc
     3901 ccaaaatagc tttcgtcgca atttgtgcca ttggaattcc tgtcacctta cttaagaatg
     3961 gaacagtccg acttgctcga ggattgactt cgatcacata aacctcattt tcatggatga
     4021 caaactggat attcatcatc ccgacacaat tcaaacctaa tgctaagcgg atcgtgtaat
     4081 cttcgatcgt tttcttgatc gattcagata atgtttgagg tggatacaca gccattgaat
     4141 cccctgaatg gacaccagca cgctcaatat gctccatgat ccctgggatg agtactgttt
     4201 caccgtcaca aatcgcatcc acttcgcatt caccaccaag caagtaacgg tccactagta
     4261 ctggatgttc tggagatgct ttcacagcat gttccatgta atcttctaaa tctcgctggt
     4321 tttcaacgat ttccatcgca cgtccgccta atacatagct tggacgaaca agaactggat
     4381 aaccaattct cgaagcgata accacggctt cttctttact tgtagcagta tcccctaaag
     4441 gttgaggaat atccaactct ttcagcgctt gctcgaataa atcacgattt tccgcacgat
     4501 ccaagtcttc gatcgttgtc cctaagattt ttacaccagc agcagtcaaa ggttcggcta
     4561 agttgattgc tgtctgacca ccaaattgaa cgattactcc tatcggctgt tctagctcga
     4621 tcacattcat cacatcttcg aaggtcaatg gctcaaaata taatttatcc gaaatagaaa
     4681 agtcagtcga aactgtttca ggattgctgt tcatgatgat tgcttcgtat cctgcctgct
     4741 ggattgcttt gacagaatgc acagtcgcat agtcaaattc aaccccttgt ccgatacgga
     4801 taggaccaga tcccagtact agaattgagg gcttttctga tcgtgaactt tcattttcat
     4861 attcatagga actataaaaa taaggtgttt ttgattcgaa ttccgctgca caagtgtcaa
     4921 ccatcttata tacaggaatc aaatcatttt cttttctcat ctggcgaacg gcttcttcgt
     4981 ttatttccca cagctccgcc acttttttgt ctgtaaaacc gttttgtttc gctgttttca
     5041 atatcttgtc atccaacggc tgctgtttca actgctgttc gatttctacg atatgcaaca
     5101 gtttatctaa aaagaataaa tcgatttttg tcaaatccgc taattcttct atggtgtacc
     5161 ctcttctaat agcttcagct acataaaaca ggcgatcatc ttgtgctcga accacttttt
     5221 ggattaggtc atcgtccgtg atgtctgcca actcaggaag ttcgatatgg tgtgtaccga
     5281 tttctaaaga acgcacagct ttcaataaag actcttcgat attccgaccg atagccatca
     5341 cttcaccagt tgctttcatc tgcgtgccta aaagccgttc tccgttttca aacttatcaa
     5401 acggccagcg aggaatttta gctacaacat aatctaatgc aggttcaaat tctgcaaaag
     5461 tcgttcctgt aacaggattt ttcatttcgt ctaatgttaa tccaagcgct attttagcag
     5521 ctagcttagc gatcggatag ccagtcgcct tacttgctaa cgcagatgaa cgagaaacac
     5581 gcggattgac ttcaatcaca taataattaa aactatgtgg atcaagagcg agttggacgt
     5641 tacagcctcc ctcgatctta agtgctcgaa taatcgaaag agaagcatca cgaagcattt
     5701 gatattcatg gtcagacaat gtttgactag gggcaaatac gatagaatca cctgtatgga
     5761 tacccaccgg gtcaaaattt tccatattgc agacaacgat tgcattatca gccgaatctc
     5821 tcatcacttc atattcaatt tctttaaaac ctgcaatact tcgttcaatc aaacattggg
     5881 tagcggggga aagacttaat ccattttctg atacgatacg taattcttct tcattttcac
     5941 acatacctcc accagttccg cctaaagtaa aggctggacg aacgatgaca ggatagccga
     6001 ttttatttgc aaaatctaca gcttcttcta ctgtattgac gatttcagac tcggggattg
     6061 gctgattcaa ttctttcatc aactgtttga atagatcacg atcttctgct tgatcgatcg
     6121 cagaaagctt tgttcctaga agttctatgt tcaattcttc taaaatacct gatgctgcca
     6181 gctccattgc catattgagg cctgtctgtc cgcctaaagt tggaaggaga gcatctggac
     6241 gctcttttcg aagaatcctt gatacaaatt caagtgtgat cggttcgata tatacttgat
     6301 ctgccacttc tttatccgtc atgatcgtcg caggattaga atttactaaa accacttcat
     6361 atccttcttc ttttaaggca aggcaggctt gtgttcctgc gtagtcaaat tctgcggctt
     6421 gaccaatgat gatcggacca gatccaatca ccataatttt cttgatatct gtacgtttcg
     6481 gcatctgttt actgctcctt tcctgcgtcc atcatttcca taaattcatc aaataaatgt
     6541 agtgcatcat gcggaccagg ggcagcatct ggatgaaact gcacactgaa agcgggataa
     6601 tctctgtgtt tgactccttc tactgttccg tcatttactt ctacatgagt tacgatgagt
     6661 ttttctggat caatcgtggc ttcatcgact gcatagccat gattttgaga agtaaaatca
     6721 attcttccgg tcgctacttc tcgtaccggg tgatttaatc ctcgatgtcc gaatttcatt
     6781 ttataggtat ctgcaccgtt ggctaaagca aataactggt gaccgagaca aataccaaaa
     6841 atcggtactt ttccttggat ttttttgatc atttcgattg cttctggcac atcttttgga
     6901 tcaccaggtc cattcgtcag catgactccg tctggtgata gcgctaaaat cgtctcggca
     6961 tctgtgttat agggaagtac tgtgaggtta cagtttcttt tactcaattc tcgtaaaata
     7021 ctatgtttca agccgaagtc aatgacaatg actttccgac ctgttcctgg acttggataa
     7081 ggtttgttag ttgagacttg ctgcacttga ttggttgcta gaacggttgc tttcagctga
     7141 tcaaatgcat gttcgaagga atctccagca tcaacgatac tagctttcat cgttccgact
     7201 tcacggattt tgcgagtaag ggcgcgcgta tcgatacctg atattccagg aattcctttt
     7261 tgtttcaaaa atgaatctag cgtcatttgg tttcgccagt ttgaagctac acgtgcatgt
     7321 tcttttacta ctactccttt gcatgttgga gaaatcgatt cataatcatc tctatttacc
     7381 ccataattac ccaccatagg ataggtaaat gtaatgattt gaccgttaaa actttgatca
     7441 gtgatagttt cttgataacc agtcatactt gttgtaaaaa cgatttctcc gaagacacta
     7501 gcatctgcac caaaagcctt tccttcgaaa actgttccat cttctaatat aagcaaacgt
     7561 tccatacttt tattctccct tctgccacac aagttttccg ttgacaaaag tcatttgtgt
     7621 ttcgcctttt actttccagc cgataaaagg tgtattctcc ccttttgata aaaagtcttc
     7681 tttatcgatc gtgcaagttt gtgtaatatc aaaaactgct acatcagcgg gggcaccaac
     7741 cgttagcgtt cctgcattca aaccaaaaat ctcagccggt ttaactgcca accaattgat
     7801 cacttgttcc aacgtgaatc gaccagtttc tacaaaatga gtatagatca gttgaaacgc
     7861 tgtttcgctt cctacgatac caaatggtga tttcatgaaa ctttggcttt tttcttctaa
     7921 tccatgggga gcatggtctg tagcgatgca atcgatcgta ccgtcaagca agccttcgat
     7981 caaagcttct ctgtcctccc gtcctcttag cggcgggttc attttccaaa agccgaaatc
     8041 ttcgggaata tcttcgtcga tcaaaataag atgatggggt gaaacttctg ctgttacgtg
     8101 gattcctgct ttttttgcgt ctctgattac acggacactt tctttagtag aaacatggca
     8161 aacatggtaa tgaacaccag ttgcttcagc tagcaacaga tctctagcaa tttgagaaga
     8221 ctcggttacg cttaagatcc cgggaagacc aagttcttct gccttcttgc cagcgtgcat
     8281 aacgccgcca aataataaag attcatcttc tgtatgggct actagggctt tattgttttt
     8341 tgcagcttct ttcatagcca gatacatcgt cccagctgtt tggacaccta caccgtcatt
     8401 tgtgaaagca aaagcacctt cttcaatcag tgcttcttga tcaactaatt tctcacttcg
     8461 cagattttct gtgattggcg catactgcaa taccttcaca actgcatctt ttctaatgat
     8521 gtcatatact tgtctcagct tttctgccgt atcaggtaca ggatttaaat tcggcatcgc
     8581 gcatacagta gtgaaaccgc cacgagcagc tgagcgagtc ccagcttcga tcgtctcctt
     8641 gtatgtaaat cctggctctc tcaaatgaac atggacgtcc actaaaccag gtgtgatcaa
     8701 ttgtcccttt gcatcaaaca cttcatcaaa ttcggcttct gagaatcctg taccgattgc
     8761 tttgattcgc ccatcttcta tccaaatttc tgctggtgtc gtcatatttt ttcgtgtatt
     8821 gatctgaccg tttttaatga gtgttttcat tttttcatcc tcctataatt acgcttttcc
     8881 atttagcact gcttctaaga ttgccattct gacgaatact ccattgctca tttgctgaac
     8941 gatccgcgac tgtaatcctt ccactaatga atcagctaat tctacatcgc ggttaactgg
     9001 tgctgggtgc ataatgattg catgtttttg caacttcttc gcacgctcaa ctgttaaccc
     9061 atgttcctga tggtagcctt cttttgagaa actttctttt ccatcatggc gttcgtgttg
     9121 cacccgaagc atcatcatga catcaacttg atctagtagt tcatccaatg gcatataatg
     9181 tccgtacact tcgaattcat cgtcgtacca ttctctagga cctgagaaga atacttgtgc
     9241 gcctagacgt tttaacatct gcatattgga tttagctacg cgagagtgag tgatatcacc
     9301 tacgatggca actttcagac cttcaaaatg accaaattct tcataaatgg tcatcaaatc
     9361 aagcaagcat tgggttggat gctgtccgct tccgtctcct ccattgataa tcgaacactg
     9421 gattgtttta ctttgtatca gttcgtcata atagttctca tcaccatgac ggataaccgc
     9481 tacgtctacg cctaatgctg acatcgtcag tactgtatcg tatagtgttt cccctttttg
     9541 gacagagctt gtgcttgctt caaattcaat cacttctagc cctagttttt tctccgctac
     9601 atcgaagctt ttatgtgtgc gagtactatt ttcgaaaaag aggtttgtag caaagtactg
     9661 gcgcttttgc ggagtccaat tagcaccttg cttgaattcc tgccctctgc ggatcaagcc
     9721 cataacttct tgatcagtaa gtgcctcaac ggtcaataaa tgctttaagc tgatacgttc
     9781 tgatttgatg atcataatgt ttcctcctta gttttcttcg cttcgtgaag tttctggtaa
     9841 gataagattt aaaatgatgc caagtacagt tgctaatgcc attgccgaca atgtaaatgt
     9901 gcctacttca aagacgagtc cgccaattcc tactactaag ataactgaag caatcaacag
     9961 atttttcttt ttatcaaaat cgattttgtt ctcgattaaa atcttcattc cacttgccgc
    10021 aattactcca aataagataa aactgattcc tgagatgact ggtccaggga tacttaaaat
    10081 caaagcgctt aattttccta cgaatcctaa aatgactgca aatactgcag ctccaccgat
    10141 cacaaacacg ctatggacac gtgtgatagc cagaacacca atgttttcac cgtaacttgt
    10201 tacaggagga cctccgacaa aggctgccac gatttgtgct aacccatcac ctgtcaacgt
    10261 cttatgaaga cccggttctt caaagaaatt acgtttagtt agtttattca ataccataag
    10321 atggccgata tgctctgtca tcgtcacaaa agcgatgggt gccattgttg tgattgctcc
    10381 aagatatagc tttggttcat attgtacaaa gggaatctca aagttaggaa cagcaaacca
    10441 ggcagcgtct acgattggct tagtatccac gattccaaac agcaaggcta cgatgtatcc
    10501 gctaatgata cctagtaaaa tcggaatcaa tccgagaaaa cctcttagcc acatgttgaa
    10561 gaaaattgtt aatcctaatg tgatcagcgc tacagcaata tacttgaaat catacgtgcc
    10621 ctgattatac atagcatttg ttgctgcatt tgccgcaagt cccaacccga taaccatgat
    10681 gactggaccg acgacgattg gaggcaagat tttatccagc catgctgaac cgatcttttt
    10741 gatgatcagc gcaacaatca aatagactaa tccagttgtg actgctcctt gtgcaatcgc
    10801 tggataacca tctgttttca tcaaaagctg catcgctgca atgaaagcga agctgcttcc
    10861 taagtacgca ggtatctttc ctttagtagt aaataagtac accaatgtac ctagtcctga
    10921 actgactagc gcaatacctg gatcgatacc tactaagatg gggactaaca cggttgctcc
    10981 gaacatcgtg aacaagtgtt gtaaacttaa tcctacccaa tgtcctgggg ttgggcgatc
    11041 gtggatatct aacaccacgt catcattatg aaatcctttt ttctccgaca tgtttctctc
    11101 tcctctaatc ttcctctttc aaaatcatga tacgatcttg tccatcaagc tcttgcatct
    11161 ctactaaaat ctcttccgcc cgagatgttg gaatgttctt cccaacataa tctgcacgga
    11221 ttggcaattc tcgatgccca cgatcgacca ggactgctaa tgaaattttt cttggtctgc
    11281 caaaatccat tactgcgtcc attgctgctc gaatcgttcg tcctgtatac aaaacatcat
    11341 caatcaaaat aacttctttt ccttctaagg aaactgggat gtcagaagaa tgaagttccg
    11401 gttcttctgg attttctttt ttgtcatctc ggtataaagt gatatccagt tctcctacag
    11461 gaatatcgat atcttctagc tgcttcaggc gttcggcaat tcttgatgca atgtagattc
    11521 cacgtgtttt gatacctact aatacaatat cttggatact atgatttctc tcgataatct
    11581 cgtaagtaat tcgagtcaat gctcgcttca tcgttacctg atctactact tcttttgctt
    11641 gcatattcat tccccctact tttttgcata aaaaaactcc tgccctaaga ggacaggagg
    11701 aatgtttaga ctgagcgtat actgatacac tgatagctat actatgtcaa tgagcaatct
    11761 acttatggcg caccatacgt atccttgctc ccacccttct tagcctcacg ggactaaact
    11821 taaaggaatt ctattaagtt ttatgctttc gttgttaatt actataccgt tatctaaatt
    11881 aaaatgcaat gttttttctt aatttttcta aagttttttc aaaaacttct ggaagaggtg
    11941 cttcaaagat catcttttcg tttgtagtcg ggtgcgtaaa acctaacatt tgcgcatgga
    12001 gaaactgacc attgcctttc aaagttttct tcggtccgta tactggatct ccagctaacg
    12061 gataaccgat atacttcata tgcacacgga tctggtgtgt tcttccggtt tctagtttta
    12121 attcaaccag agtaaatcct tcgaaacgtt ccaatacagt aaaatgtgtg acagccggct
    12181 tcccatcttc tcttatcgcc tgcattttcc ggttttcctt cgaacggccg attggcgcat
    12241 tgatggttcc tttttcgtga ggaatctctc catggactaa agcaacatat ttgcgcaaag
    12301 aggtcttatc ttttaattgc tgagctaatg attcgtgtgc ctggtcattt ttggcaacca
    12361 ttagcagtcc ggaagtatct ttgtcgattc ggtgaacaat acctggacga ataacgccgt
    12421 tgatcgtaga aagatccttg atatggtaca tcagcgcatt gaccattgtg ccatgcgtat
    12481 gacctgctga aggatgaacg accattcctt gaggtttgtt tatcactgct acatcttcat
    12541 cttcgtaaac gatcgtcaac gggatatttt ctgggaccac atctaatgtt tctggttcgg
    12601 ggatcaagac agtaatctcg tctccttgtt ttactttgta atttgctttg actggctgtc
    12661 cattgacaga aaccgctcca tttttcagcc acagctgaat ttgcgaacga ctgtaatcgg
    12721 tgaataaatt agtcaatact ttatcgatcc taccagattc atggtgaatc gtcgttttaa
    12781 gttctgtcat ttttttcttt gatccttttc atctaaaata atataaatta atatacaaat
    12841 cactccacat acaagtgtca catctgctac gttaaaaatc ggaaagctca taaaatctgt
    12901 ctggaacata tctactacat agccaagacg aatacgatcg ataaaattgc caatcgcacc
    12961 agctagaacc aaactcaatc caatcatgaa ccagcgatta cctttttcac gatttttaat
    13021 catcaatgtg agaatcacaa cgactgcaat gagtgtcact atagtaaaga accacatctt
    13081 cccttctagc atactccatg cggctccagt gtttcgaata tgattcagtg acaaaatacc
    13141 tggtataaga tttttcgtct ctccaagcgc tagattatca gtgatccacc atttgaccaa
    13201 ctgatccact cctactagca aagcgctgaa tatccaataa ataattaaca aaaaatcaat
    13261 ccttttctat ttgtttataa atatcacgag gtcgtacgat cccgagcaga ttaccatcgg
    13321 gattcccatc tttagtgata agtacggctt ccaaagtccc ttgctcacga aacatcgctt
    13381 caacctcgta aatatgtgtt ttagctggaa caaaatgata atttgtgtac tttccgtttt
    13441 ggatgatcaa atccaacgct cgtttatttg ttaaatcaac cggacctttt tggctttctt
    13501 ttgctagcca aaacccgata ttacgtaaag taatcaatgc tacaaatttt cctttattgt
    13561 ataacggaaa ttgtgaatac cttttttctg ctactatctt taacagttca agtaacggaa
    13621 gatcttgttc aaaacctgtt acacgttttg caaatcgagg cagcacggtt tcaggctgaa
    13681 tcagttcttg ttcgatcagc tggattcgct tgactgtcca atcatttggc tctgcaatga
    13741 caaaatcgac tgcgatccga tcatggacga tcgcattcct taactgagca agctgtaaaa
    13801 gatcatcttc gtattttctg atcattaatt gttttctttg agctaggcgt ctgacaagtt
    13861 cggtaaatcc catatttcgt gcatttccca tctcgtctct catccatttt tcgattcgat
    13921 tgaaactgat taaaaaaaga tcagagtttg ccataatttt cctccttgat caaatatgcc
    13981 cggtttttta tatagaaaaa gaacgcaatc aacacaataa ctgcaaaaaa actgaaaaat
    14041 aaattttgta ggaaaacggc ccccaaccca gttgctagaa ataaaaacgg atagatttga
    14101 agaatcaagt aattcattgt gatcacataa gcaggctttg cttctttcac tgtataaacg
    14161 gggcttttta gccaataaaa ggtgacctgc atcgtaaagg gcacttccag atcaagcgtt
    14221 atcttttttt catgactgag aacactgcgt ttttcatcgt tgattcttag ctgaatcgag
    14281 ctacccatac cgtaaaaacc ggtttttctt ataaatgtcg ttttcatcat accctcctat
    14341 tatgctttag tatagctcaa aatacaataa agaaaaagaa tgatcctagt gtaaacaaaa
    14401 ttaattccct atcgtctaaa aaaagaagct gtagtcaaaa tacagctc
//
LOCUS       DACECY010000065        14197 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.069, whole genome shotgun sequence.
ACCESSION   DACECY010000065 DACECY010000000
VERSION     DACECY010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.069"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1302)
                     /locus_tag="IWO38_002187"
     CDS             complement(<1..1302)
                     /locus_tag="IWO38_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0314265.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFK"
     gene            complement(1299..1952)
                     /locus_tag="IWO38_002188"
     CDS             complement(1299..1952)
                     /locus_tag="IWO38_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0314266.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2240..3173
                     /locus_tag="IWO38_002189"
                     /pseudo
     CDS             2240..3173
                     /locus_tag="IWO38_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3081..3797)
                     /locus_tag="IWO38_002190"
     CDS             complement(3081..3797)
                     /locus_tag="IWO38_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ0314267.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(3847..4056)
                     /locus_tag="IWO38_002191"
     CDS             complement(3847..4056)
                     /locus_tag="IWO38_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314268.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            4333..5606
                     /locus_tag="IWO38_002192"
                     /pseudo
     CDS             4333..5606
                     /locus_tag="IWO38_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5713..6744)
                     /locus_tag="IWO38_002193"
     CDS             complement(5713..6744)
                     /locus_tag="IWO38_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0314269.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6751..7581)
                     /locus_tag="IWO38_002194"
     CDS             complement(6751..7581)
                     /locus_tag="IWO38_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0314270.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7578..8384)
                     /locus_tag="IWO38_002195"
     CDS             complement(7578..8384)
                     /locus_tag="IWO38_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0314271.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8390..9214)
                     /locus_tag="IWO38_002196"
     CDS             complement(8390..9214)
                     /locus_tag="IWO38_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAQ0314272.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(9204..10304)
                     /locus_tag="IWO38_002197"
     CDS             complement(9204..10304)
                     /locus_tag="IWO38_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0314273.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10482..11267
                     /locus_tag="IWO38_002198"
     CDS             10482..11267
                     /locus_tag="IWO38_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAQ0314274.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            11300..11683
                     /locus_tag="IWO38_002199"
     CDS             11300..11683
                     /locus_tag="IWO38_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ0314275.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11759..11890
                     /locus_tag="IWO38_002200"
     CDS             11759..11890
                     /locus_tag="IWO38_002200"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0314276.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11859..12095
                     /locus_tag="IWO38_002201"
     CDS             11859..12095
                     /locus_tag="IWO38_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0314277.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(12173..13144)
                     /locus_tag="IWO38_002202"
     CDS             complement(12173..13144)
                     /locus_tag="IWO38_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ0314278.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            13778..>14197
                     /locus_tag="IWO38_002203"
     CDS             13778..>14197
                     /locus_tag="IWO38_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0314279.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVSQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRQFRETTVFEDIFAYILEQAVK"
BASE COUNT         4289 a         3093 c         2483 g         4332 t
ORIGIN      
        1 tttaaagata aaaaattctt catcctgcaa tactttatat tctatattgt aattattcag
       61 aatattactt acaatatata aacctaatcc attactattt tccttattta aatcaaattt
      121 aacatcaaat attttatcca tattcgaaat tttattgtta ccgtatgaat tctctatata
      181 taaccaatca ttaactatcc caatattaat taccccattt acatcagtat attttaccgc
      241 attactaatc aaattagata gaataatctt taaagctgtt tttcctatat aaacattctc
      301 gtctaatata taattattga cagttatttt cttttgattt gctaatattt catacttttt
      361 taatatgtct tctaatgtat catttatttt caaatattct tcatcattat tcaaattttc
      421 tatagaatga actgataata tttgagaaat attttttgtt aaactatcaa cgatttcaat
      481 acattcatta atataaatat ctctttcttt atattttcca atattgtatt tcatgttttc
      541 cagtattatt ttaagactag caagaggggt tttaagttca tgggatgcac ctttaaaaaa
      601 gtcatatttt aacttttcta attttaaaat ttctttattt ttaaattcca aatcgtcaat
      661 tgttcttaat aaagtagaat acaaatcatt aatttgttgt tttaattctc caacttcatc
      721 attagagcta acttttaaac tcattttttt atcaagtttc atcattttat cagtaacaat
      781 ttttatttct tgtatattat tttttattga ttttgcataa attaaagaaa taatagcaga
      841 aaataaaatt gatattaata aagaatatgg taaaaattta aggcttaaat ctttcgcatc
      901 tttttgcata tcagctgtag aaacaaattg tagatttgtt tttttaccat cattaagctt
      961 tatttctctt tcttcaatta tcaaagaatt actatcactt tctaaattaa catttatatt
     1021 atctttgatt tgtatttcat tcttattatt tttttctttt atgaatactt ttatttcact
     1081 actcttggaa taaaactcca aagtttgttc tatatatttt atttcttttc cattcatatt
     1141 actagaaatt tcatttgcca tattatgaat cttttctttt ctcgtctcca aatatgtttt
     1201 aggaaagata aaaaaaatta atgaatgaac taatataatt attattccaa gaacagaaaa
     1261 tatttgtatg aacatttttg gaaatatctt taattttttc attttctctc caatttatat
     1321 cctacatttc ttatagtagt tatacagtct aattgtaatt tttttctaag ttcttttata
     1381 tatacatcta ttactcgatc ataaggagtt tcttcgctat ctttccatac ataatcaata
     1441 atttgcattc ttgttaacac ttgtccatta ttatccaata aacattttaa tacttctagt
     1501 tcttttgcat ttacatctat ttcttcacca tttattttag ctgtatagct attaaaatta
     1561 accgatagat ccttatattc aaacttctct aaatgtccat aattcttctt aattaaagaa
     1621 tctattctag cttttaagac cggcaatgaa aatggctttt ctacatatcc gtctactaaa
     1681 ttagtaaatg catcaatttt atattcttca tcactaaatg cagtcaagat tagaattggt
     1741 aaattgcttt tctttctaat ttctttcaac acttctaaac catttataaa aggtatctga
     1801 atgtctaaga taaccaaatt tatatcgcta ttaaattttg acaaggcttc tcttccatct
     1861 ttagcttgaa taacagtata tccaaattct gaaagatatt cacttattcc ctctcttatc
     1921 atattatcat cttcaacagt taatattttc atatatacct ccacttaagc ctatacacta
     1981 gatattatac agcatattat ggctctataa tatctagtgt tggtgacgcc ttctgttgcc
     2041 aatacttttt ctttttttta ataaatgcaa acagacacct tgaaatcctt tagaaaaaag
     2101 gcttctataa tattttgtgt tgttggaaaa tcgaaggcga ttttctacta acactttttc
     2161 ttcgtttcaa ctcaaaaaga gactcacttt tggtaaaatt tagtcgacca aaacatcacc
     2221 ctaccaaagg agtcgctcta tggataatca tactagaaaa ttactcggat taacagataa
     2281 acatttattt tttgatgaag aatggctgat tgaaaaagaa tataaaggcg ttcaagcgca
     2341 gtttattaaa gggagattgg atgactcacc ttcccattgt gaacactgtg gatcgattcg
     2401 tattatccgc aacggttcct atacgacacg tactcagatg ttgaaagtca aagaaaagtt
     2461 gactatttta gaattaaaac gtacccgttt tctatgctat gattgtgggc agaccttttc
     2521 tgcgaaaacg gacttagttg atgaacatca tcaactaaca aaggaactga agcaggcgat
     2581 tctcatagaa ctgtacgaaa atcaatcacg taaactaatc gctaagaagt acttcgtctc
     2641 ggatgggacg gtgacacgga tcttgcgtga agcaacgaag cattaccagc cacgtatgaa
     2701 cttccttccg acggtgttgt gtatggatga gttcaaatca atgaagtctg tttctagttc
     2761 catgagtttt atttgtgttg atgggacaac ccatcagcta tttacgatcc ttgaagatcg
     2821 cagactttac aaactaactc aatacttcct gcactttcct agaaaagccc gtttaaaggt
     2881 caagtaccta gaatgccagc tattgtcagc tgcttaaaac ggtctttcca aatgcggaga
     2941 tcgtaactga tcgctttcat attgtccaac atatcaatcg cagcttcaat caattgcgta
     3001 tccagatcat gaatcgtttt cgagcctcgc actctgaaaa tcagaagaag taccgtcggc
     3061 tcaagcgata ttggaaactc ttactcaaag attccacgac attggagccg ctcaaacggc
     3121 attatcatcg actgtttaaa cgaccaatta gtcaaacaga aatcgtcgat gaaccgttgt
     3181 gcacaatggt gtcctgaacg tacagcgaca ccttccatat ccagagcagt cgccacatca
     3241 cggggatgga tgccatccaa attaaaagca accacgccag tatgatgttc aggatcttgc
     3301 ggtccgtaaa tggtgatacc gccgatcgct tgtaatttag gtaaaacgta ggcaaccagt
     3361 tcttgctcat aacgatgaat attctccata ccaatctcat tcaagtaatc gattgctcct
     3421 gcaatgttcg gagtaccagc ttcaaatttc catggcagct ctttccatga actatcgtaa
     3481 agattgacaa aatctatcat ttcaccgccg aattcgactg gctccatttg ttctaacaat
     3541 tgacgtttgc catataatac accgattcct gtcggcccgc acattttatg cccgctaaag
     3601 gcgaaaaaat ctgcatccaa cgcttgtaca tcaactgtca tatgcggtgc tgcttgggca
     3661 ccgactggac tatcctcaac tacaacagcc tgttcaaccc tcagccgttt caagcacaac
     3721 aaataacctt ctggatctgg tttgccgacc tgaagatcat cgatcgtcac aaaggaatca
     3781 attttttcct taatcatcgg atgtgcttca aaaaaatagt ctagctcccg cagttcagaa
     3841 ttgagactac tccgattttc cagtgctgct gatccgctgc tcaaaatgtt cagcatccac
     3901 tggtctccca accgcttgac ttatcccttc ataaaaggac agttctgctg ccgtcccaac
     3961 accttgttga tagtctgcaa aaggaatttt atagccgaac tgctccagta aataggcttc
     4021 agtaaatagt gatgaatact ctcgtaaaac atccattcac tgtcaatcat cgtcccatca
     4081 aaatcaaaaa taagtgcttt ggtcatcttc tttccccctt acccaaaaca agcaaaagct
     4141 gtgtcctgct ctctctacag tatcagcata aaaactgccg tctgaaaagt caaaggtgaa
     4201 agaaatgtgg cgctataatt tctaggactg atagtaaatc attctatcag tccttttgag
     4261 cataaaaaaa caaaacatac agaagctccg ttagaattga atcaccacaa cacaatcaaa
     4321 ggagcgttct gtatgcctga tcttcatctt atcaaactat tactaggaat caaagatgga
     4381 aacattcaaa tcactcatgt cgagaagaaa aaaatacatt caatcaacag tatcgttgtt
     4441 tctgccacgc tcattaaaac gattcagccc tgtcctcagt gtaaacaggt gaatcaacat
     4501 gggctgatcg ttaagaacgg aaagaaaaaa tcgctggtcc ttctcaataa gtgtgcgaat
     4561 caacggactt atcttgcttt ggcaaagcaa cggtacctct gtcgtggctg tcacacctat
     4621 tttacggcga atacctacat tgttgaaaaa aactgtttta tcgccaaaca agtacattat
     4681 aaaatattgg aggaattggc tgaaaaacaa gcaatgacga cgatttctaa acattgcggc
     4741 gtttcttggt caaccgtttc gcgaacactc gcttctctgc tccccatgac aaacgtgaaa
     4801 ataaattggt tgcctcgctg ttcattggta gatgaatttc gttcattgaa aaatcaagtc
     4861 ggtccctatt ctttttcttg tatggatggc gatactggga aattactgga tattcttcct
     4921 tcacgtaaga agaaagatct catttcttat tttatgcaat ttgaacgacg tgcacgttta
     4981 aacgtaaaaa tactcgtaac agatatgaac gcctcttatg ctttgctgat aaaagagtgc
     5041 tttcctcatg ccaaactcgt tgtggatcgc tttcacatcg tcaagcacct cattcgcagt
     5101 tttaaggata tttgactatg agtcatgaaa agcttcgacc gaaacgatcc aatacaagcc
     5161 aaacactatc gacaagtgaa ggctttatct cgacttctga tcaaacgaca ggacacgctt
     5221 gtttatgata aatggacaaa atggcgtaat tttggttggg cctatttaac cgaaagtgaa
     5281 gtcgtagaac gtctactttc tacttctgat gaactacgaa tagcttatgc ttactatcaa
     5341 gaaattcttc aagcttttta tgacaaagaa gcggacatct tctttcagct tgtaaaaaca
     5401 atgcccaagt cggttccaag agaactgcat catataaaaa aagcgtttat aaactatgaa
     5461 tccggcattc gtttagcctt agagttgcct tactcaaatg gcaaaataga aaatctgcat
     5521 acccacatca aagcattgaa acgaatagct tacgggttag gatgagaacc agaattttct
     5581 taatcaatca tctgatcact tattaaacat aaaaaaacat ctaaagcaga aaatcaattc
     5641 taccttagat gttcttatat cactctatca gtcctatttg acaaagagcc agaaatgttt
     5701 gccgactata cactagcttt gttgtgcaaa atgctgagta tcaatttcca cagggattgc
     5761 tagaatatct ttcagctgaa tgtataaggc atcgctaatt ttctcttccc gatcgttcaa
     5821 ccgatccact aaaatttctt tgccctccag cgtttgaaaa acataccgtt gaatgttgcc
     5881 taaaataaat gattccttcc atagaaccgg aatcttaacc ccctgaggaa ccggttcaaa
     5941 ttgaatcagc tccggtctta aataataaat gcagcgattg cccttagcgg aaaactcgtg
     6001 ggtatactga ttcagctcat accattcgaa acgattatag ttgccgataa attccgcaac
     6061 aaatcggctc tgaggattct gatacaattc cttcgaggtg gaaacctgag ccacccgccc
     6121 agcttccatc acaaaaatcc gatcactgat tcgcatcgcc tcttcttggt catgagtcac
     6181 aaaaatcatc gtcatcccta gcgattgctg gatagcgcgc aaatcccgct gcagctgttt
     6241 tctaattcgg gcatccaaag cacttaatgg ctcatccagc agcaggactt tcggttcagt
     6301 gaccaatgag cgagccagtg ccacccgttg tcgttgaccg ccagaaagaa actggacatt
     6361 tttatctgcc tgctccgtca tatccaccaa tgatagcatc tcaaaaacct tttcctgaat
     6421 cacctcagga ctcagcttct ttaccttcaa accaaaggct acattttcaa aaacagtcat
     6481 agttggaaac aatgcatact gttgaaaaac aaagccgata ttgcgatcct tcgtaggctt
     6541 atcggcaata ttctcattgt ccagcacaat ctcaccgctc tttggttgtt ccaatcccgt
     6601 gatacagcgc agcaacgtag actttccgca cccgctggga cctagtaaag taaccagctc
     6661 acccttttca atctgaattt catcaatagc taaaatctgt ttttcatcaa aaaacagatt
     6721 gagatcatgt atttctaaaa acattttccg tcacaactcc tttaagggct tctttttcga
     6781 ctgcttctct tccctcgtct gcttaaggga cagctgttgc ttttgtcgat aagtcagcat
     6841 aaaggtagtt aaagcaattc ccaccataaa cagcaaatac aaaaccatca cagcgctagc
     6901 caaatggccg ttttcgttca tccgccgcag catatagatg cgtaccgtct gaaatcgacc
     6961 gccaatcaac aggttcgtca gcatgtactc cccaaaaacc gaagaaaaaa tcatcagaga
     7021 aactagggtc acaccgatac ggatgtttgg aaacagcacc tggataataa tcgtagacat
     7081 cggggcgccc aaggtttctg ctgcatccac taattccttc agattaatta agcgcatctg
     7141 gttgttaaga ctaagataga caatcggcaa cgccgtaata aacaatgcac ctaccaacac
     7201 tacaaacatt gggataccag tttcagcata cgttcgaagc aaagccgtta ccaaaatcac
     7261 tcctggtaaa gcatacggca gcaggagcag cttttccagc caacgattca aacgagggaa
     7321 atacaaatga atccaaataa tggtgggaat catcaacaac agaatcgtca ccaagaccac
     7381 acctgctaat aaaagtgagc gacccaccgc agcaataaat tccctgtccg tcagcagctg
     7441 ttggaaccat tgcaaggtaa aatcactagg taaaagcgat tttgaccatt tagtggagct
     7501 ggcatacaaa atcgttccaa taatcggcag caagtaatac aggacaaagc agaacaaaat
     7561 cggttttatt aactttttca cgtctgcacc accttgcctt tcttttgaat aatcaacatt
     7621 gccgttacca gcaggacacc taaagcgact gccaacgcac tgcctaaatt aggcttggcg
     7681 aacacatctc ccgacactaa agccgcaatt cgaatcgtta aaagatttac attactgctg
     7741 gtcagggcat acgctgtttc ataggtcccc atgccattag caaataagat gacaaaggtg
     7801 ctggcaatcg atggtcgcaa aaaaggcaaa gccaccttga accaagcata cgctgatgaa
     7861 gcgcccagca attgtgcgga ctccagccat tcctttttca cttccttcat gctgggatac
     7921 aaaaacaaga cccctaacgg aatttggaaa taggtatacg tgatggtcaa tccccaccaa
     7981 gaatacaaat tgaagtccgc tagaaatggc aaaatcattt tcagcacacc tacattcccc
     8041 aacaaaataa taaaagagaa tgccagcgga atcccagcaa aattagctgc caaattggaa
     8101 acaaaggtga ttttctcctg caatttagtc gatagattca ttaagcataa acataaaata
     8161 aacgtcccga ttaacccaat cacactggag ctgacagcaa tcagtacgct gttaacaaag
     8221 gcctgtgtgt aatatttgtt gctgaaaata tcactgtaat tggccaatgt ccactggtta
     8281 gttaaatcac tgtgcaagct gcacagaacc ataaaaagca gcggcagcag taaaaatgca
     8341 cagaagataa aggaggcagg aaccagaacc aagcgttttt tcatctcttt catcccctca
     8401 tcaattcttg aatgtcctgc attttctctg acggtgcaat tcctaataac cagcaaaaca
     8461 atggagccgc cagcagctgt ggtacagttt gttccttcag cttttggcgt ttatttgaaa
     8521 tcaaataaaa aggaacttca cgatgtgcag ccaagctgcc gccatgcagt ccgtgcgcat
     8581 ccatcccgtg atccgcagtc accacgactt gataaccttt ttgcagccat tcaggaaggt
     8641 aaatagccaa taatctgtcc gcctgattaa ccgctgctgc atattcatga gaatcggcgg
     8701 taaatttgtg accgacatca tcaatattca tcgaatgtac gaccaaaaag tctggctgat
     8761 actgattcac taaataatgg gcatccgcaa ataaatgcga atcagggtaa gtgtcctcac
     8821 tataaaagat gccatgctga atcagtcgct cctcctgatg ctggatgcga tcaaccaaac
     8881 gattataggg ggcttgattg tacaactcgc tgtaccagtg gtaagcggcg gctcctgttt
     8941 ttccgccagc ttccttaact aattcaaaca gcgaccactc tttggatcgc tgagcatggt
     9001 aattactata gatgccattt tcataggctg gaacgccggt catcagtact tcgtataacg
     9061 gacgtgaatt gctgggcagc tcacttttta ccttcaataa gcttgcttgc tggtgctcaa
     9121 ctaaatgatt catgaaccca agctgggcga cggctgtgtc gtagcgacag ccgtctagta
     9181 taactacaat cagtttgtca agtctacttt gcataggtca gtacctccgc ctgccaaagc
     9241 tctggaagct ctacagaggt cttgttccaa gcttcattgt cttcaactgg caccgccgct
     9301 tcataagcat cctccggcag taatttttca gctacttctt taggcagctc taccttgcga
     9361 ataggtcgcg catagccctt tgccaaattg atttgccctt catcagataa aatatattcc
     9421 cgtgctagct ttgccgcatt gggatgaggg gcatttttat tgattaaggt tgtatacccg
     9481 ctaatgatgg agccgtctgt tggcacagtt acctcaaaac gctcggagtc aatttggtca
     9541 cgataattta gtccattgaa gtcccaaaca actgccacat cgatttcacc tttttcaaga
     9601 tttgctacgc tggcatcaac ggtggacaaa cggccttttt ctgccatttt tttgtagtaa
     9661 ttcagacctg gctgaatatc cttttcgtca cctccgttgg caatcgccgc agccaataca
     9721 gcaaattgtg cttggttggc aatcgtcaca tcccccacgc taacttgata gtcaccctct
     9781 tgcagctcct tccagctagt cggtgcttgc ttcacattct ctttatcggt gataaaggaa
     9841 atggttcccg tatagcttaa caaccaatcc ccatcatcat cctttgccca atccggaatt
     9901 tcctcccaat agttggtctt ataaggtaag gttaactctt tgctttcagc caacggaccg
     9961 aagctgatac cgacatcacc aatatcggcg gttccatttt ttccttctga ttcaaactta
    10021 gccaattctt ctgcactgga catatccgta tccacgtgac ttaagccgta ttctttttca
    10081 aggtcgttcc aagtgcctac ccaatttgcc caagtatccg gcattcccaa gctggcaatc
    10141 tctccttctt ccttggcttt ttctataatc gtatctaaag agtcgtctgc cgcagccgat
    10201 tgcttcgttg cttcacctga gccgcagcct gtcagcagga tacttactgt tgctgcacaa
    10261 aataaaccga tagtcatgtt acgaattttt tcattcattt tcattcattc ttctcccctt
    10321 tcgataattc cattctaaca aagctttgta atgagagaat cagtttcttg aaaaggtttc
    10381 atgaacaatg taaaggttgt atatggtttg taaatttcga cgaattcccg ttaaccttct
    10441 aaagcttttg cggtatgata gtagaaaaaa ggaggctccc tatgcagtat tatgatcagc
    10501 atctccacac gtatttttcc tttgattcag aggaaaaatt tgaaaactat ttagcctatc
    10561 agccggagtt tttcgtttct acggatcact ttgatttaaa caatccatct accggaaaag
    10621 atgacattcc ggattatcca gcctatataa acaaactgga ctctttaaaa gaaacctatt
    10681 ccaccaaatt tctgcgaggt atcgagattg gcgttgttcc aggacaagaa gagcggatcg
    10741 tggattacct cgctcagcat ccttatgatt taaagctggt aagtattcat caaaatggcc
    10801 gctttgatta tatggacgat gtggttttaa ccaaagatcc cattcaaact accaaggaat
    10861 attttgatca gatgttgcag gtcttgacgg attttccaga aggagatatt ttgactcact
    10921 ttgattatgg attacgccgc ttcaccttca cagcggaaga attcgcggag cattttgagg
    10981 ctcagctgct aacgattttc aaaaaggttg ttgaacgaga attggcgatg gagttaaatg
    11041 ccaagagctt cttgaaatac ggcaacgaag acctttaccg ttatgccgtt cctttgtatc
    11101 aaagtgtcgg cggaaagctc tttaccttag gctcggatgc tcatgttgcc gaagattatc
    11161 agctgggctt taaaaaaatg agtcagctgt tggcagactg tcaggtagaa aagttgctag
    11221 tcattcaagg aaaagaacgc cgcctgacgc cgctgccgat tttgtgattc ctatataata
    11281 gaagaaagtg gtgaaaaaaa tgagcaacct gtgggactac aatcaagagg cacccatcca
    11341 ttatttaatt gcgcgacatt gggatgcgtt gaaaattgag gcggtttgcc gatccttgtt
    11401 agcagccgtt ccaaaacaac aactggaaaa ttttttggtg gctgacagct tgcagcggga
    11461 aaaagttcag gcttattttg cagcctttaa agatcagcct ttggaatatt tgcatgcgca
    11521 gtttcatctt ttttatcaag tagccgcccc agatgactac aacgatttac gaggacagct
    11581 gcaattgacc tttcaagcgg atgaaaccgc ctataccgtt ttattgggaa tggctcgcct
    11641 tggtgatcag gcgaaggtcg agtggcggat tttcgatatt tgatggcagg atcaaggcgt
    11701 ttcagttgca aaccttcctc tctttcaatt agaaaaaccg cagcaggaag aagtttgtgt
    11761 gctggtcacc gatgctggtg aaattcgtct ccgcttattt cctgaccaag ctcccctggc
    11821 tgtagccaac tggcgctccc tagctgagca aggcgtttat gacaacacac ctttcggctc
    11881 gtgtcattaa ggaatttgtt attcaaggtg gcgctttgga tggttctggt gatgaagctg
    11941 tttccagcta tgagggcttt ttcgctgatg aagtgcatcg cggactttat cattttaatg
    12001 gcgccttggc cttaggggac cacggccccc acaccaacgg caaccagttt tttattgtgc
    12061 aaaacacaaa ggcgcaggct gacctgctaa tgtaaagcaa gcatacttgg agcttggagg
    12121 actaccggag ctggatggtc gttatactgt ttttggacag gtataccaag ggttagaaat
    12181 cgtagcagcg atcgcagctc agccgacaaa cagtgaagat gtaccgattg agccagtgtt
    12241 cattcgacaa attaacttcg aaaaaacatc gtaaaaagac actcactgac ttcagcaaaa
    12301 gctctattct tgagctggct gcagctagtg agtgtctttc atttatccat aagataaagc
    12361 ttggattcaa taaataaata aatgttttgg ttaaagctgt gaccagcaaa tgaggaaccg
    12421 tgcgagccag ttgatctgcc aatgcaaaca tttctttagg tgaaggcgct ggcgtgtctg
    12481 ccaacggata ctccagtccc aatgcttggt aaccgtgcat accatagcag tggtacggta
    12541 gtaattctaa cggtacgaac cgatcaagat ctgccacaaa acaagcaatc gtcagcagct
    12601 cggtcaggtt catattgata cctggaacca caggaactcg aattcgcact tcgcagttag
    12661 gatactcaat taaaaagcga cgaatattct tcaaaatttg ttggttatct aagccggtcc
    12721 attgtttgtg gagcttgctg gtaaacattt ttacatccac aaacaatgta tccacatagg
    12781 gagctacctt ttggatttca ttataagcac cacaaaagct ggtttccagt acggtattga
    12841 ttccaatgta tttcgatttt tgcaaaattt ctttagcaaa atccgcctga accagtgcct
    12901 cgcctccgct aatggtcact ccgccatccg agtgaaagta aaaaacagca tccttctcaa
    12961 tctcatccat tacctcttca gcggtcatgg tctggccaaa tcctttttca atctgcttgc
    13021 tttgtgattc gggaatcgaa caccaggcac agcgtaatgg gcagcccttt aaataaacaa
    13081 cggtccgtaa accagcacca tcatgtagag aaccctgttc tattcttaat actgttcctt
    13141 ccatatagat ttttgaatta cacactagtt taaactaaaa cacacaagga aaaacttacc
    13201 atttaatcat aaaattagct catataaata tactaggatg taaaatagac tggtcaaaat
    13261 ctggtcaaaa aagaggacta atacaggtag tcctcttttt tgttatttta atattctaaa
    13321 tcatttttta ttggtttatt cccttattcg atgtagactc aaatcactat atcactagat
    13381 atcaaacggc gtcatacgga atcaaaaaca tcaaatttga cctgttaaat ggtcattgaa
    13441 taacattcaa aatcaataaa acggagtcag acacggagtc aaaaacgctg agagtaaaaa
    13501 atatatacag ccttgtaggt aaagtaccac agggctgtat attttttatt tcagattacc
    13561 ttaaatgatt ccatttactt tttatctcat tttttcctac tagttagtat caaatactct
    13621 cctattttct caataaacta tttttggata catggtatct gaaacagcta aaaccttgtc
    13681 attacagcaa aaaatcacgt tatccagact gtagacaaag tccattttat ggatgatgtc
    13741 tacagtcttt tcttttataa tagatttatg aaaacgaatg caaggagtga tagctatgat
    13801 gtctaaaaac aatacgaatg gtagaaatca atttgcgatg cttacgattg atgatctagt
    13861 ttctcaagat catctcgttc gaaagattga tgccgcgctt gattttgaat ttatttatcc
    13921 aatcgtcgaa gccacttact ccgatttagg tcgcccaagt atcgatccag tgattctgat
    13981 caaattggtg tttattcagt atttattcgg tatccgttcc atgcgacaaa ccattaagga
    14041 agtcgatacc aatgtcgctt accgctggtt tttaggctac tccttcgagg agaaaatccc
    14101 ccatttctca acttttggaa agaattatgt tcgccaattt agagaaacga cggtgtttga
    14161 ggatattttt gcctatattt tagaacaagc agttaaa
//
LOCUS       DACECY010000066        14115 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.070, whole genome shotgun sequence.
ACCESSION   DACECY010000066 DACECY010000000
VERSION     DACECY010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.070"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..554)
                     /locus_tag="IWO38_002204"
     CDS             complement(<1..554)
                     /locus_tag="IWO38_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ0314280.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
     gene            complement(570..1049)
                     /locus_tag="IWO38_002205"
     CDS             complement(570..1049)
                     /locus_tag="IWO38_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ0314281.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(1055..2079)
                     /locus_tag="IWO38_002206"
                     /pseudo
     CDS             complement(1055..2079)
                     /locus_tag="IWO38_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(2096..3202)
                     /locus_tag="IWO38_002207"
     CDS             complement(2096..3202)
                     /locus_tag="IWO38_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ0314282.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(3431..6028)
                     /gene="adhE"
                     /locus_tag="IWO38_002208"
                     /gene_synonym="adhC"
     CDS             complement(3431..6028)
                     /gene="adhE"
                     /locus_tag="IWO38_002208"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ0314283.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(6333..6713)
                     /gene="yajC"
                     /locus_tag="IWO38_002209"
     CDS             complement(6333..6713)
                     /gene="yajC"
                     /locus_tag="IWO38_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ0314284.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(6810..7955)
                     /gene="tgt"
                     /locus_tag="IWO38_002210"
     CDS             complement(6810..7955)
                     /gene="tgt"
                     /locus_tag="IWO38_002210"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ0314285.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            8173..8871
                     /locus_tag="IWO38_002211"
     CDS             8173..8871
                     /locus_tag="IWO38_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ0314286.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(9039..11678)
                     /locus_tag="IWO38_002212"
     CDS             complement(9039..11678)
                     /locus_tag="IWO38_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0314287.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(12297..13328)
                     /gene="queA"
                     /locus_tag="IWO38_002213"
     CDS             complement(12297..13328)
                     /gene="queA"
                     /locus_tag="IWO38_002213"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ0314288.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            13521..>14115
                     /locus_tag="IWO38_002214"
     CDS             13521..>14115
                     /locus_tag="IWO38_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0314289.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDE"
BASE COUNT         3957 a         3095 c         2543 g         4520 t
ORIGIN      
        1 gcgcctacga gataacctcc cataaacaaa gcagagatcg aagcaccacc agcaacaagt
       61 gaatacatga taaaagcagt acttggagga atgaccatac ctgtcggagc tgacgcgata
      121 ttgaccgctg tcgcaaactt ccggtcatat ccttcttcca cttgctgagg gatcaacact
      181 ccaccaatcg cggtagaagc ggcaatggcc gagctagaga tggaaccaaa taaagcattc
      241 cctaacacat tcgtatgagc caaagccccc ggcacgcgcc cgacgaacaa catcgcaaaa
      301 tccactagtt tcttagctat ccctccatta ttcatgataa tgcccgagag aacgaaaaac
      361 ggaatagcga tcagcgaaaa actattgata cttcccacca ttttttgagc tgaactaaaa
      421 gcagaaacat caaacggtat aactaacaat aaagtcatca tcgatgagaa tgcaatactt
      481 atagcaatcg gcatccctaa tacaagcaac acaacaaata caaaaaataa cactaatccg
      541 gcttctattg ccattttatt cctccatttt tatatttttt cttgaattgc tggcttgttg
      601 cttaatgtat cgattagatt gatcaggcta tagataatga tgagtacccc cgatacaggt
      661 actgacagat aaacatatcc cataggaata cttaaagaag gcgagatctg ccccattgtc
      721 aatgtgacag cattccatcc gccatacacc atgatcatcg cagcaaatac cagaaataaa
      781 ctttgaacaa caacttctaa aattttactt tttttgccag ttaatttttc acttaaaacc
      841 gttacggcta aacgtgcttt ttgtcccaca acatacgcgg aagacaacat ggcaaaccag
      901 acaagaccga atcgaacaag ctcttccgtg accgtatttg gatttcctaa aaccgttctg
      961 gaaaatactt gccataaaac aacagcgacc atcccaacaa gcaaaacagc tcccaggatc
     1021 tctaatacct tgtttaaaat cattttcatt acagctacgc tccttgctct tggatctttt
     1081 gataaaggtc tttgtaggta ggatcgtttt tgaatatatc atgaagtggg gcaactgcct
     1141 ctcgaaacgg tgtattatct acatcattga attggactcc atgttcttct tgtgctaaag
     1201 ctttttcttc ttcaattgct tttgcaaaag cttcaatttc aaaaaccgtt gattctttcg
     1261 ctgcttcttc aatggctttt tcctgttcat cagttagctg ttctttgatg gcatcattca
     1321 tgatgatgat atctggcaca cgtgtatgct catcatagga atagtattta gctaccccgc
     1381 catgtccagc tgtatacaag acaaattcat tgttttctga tccgttgatc aaattggatt
     1441 gaagagacgt gtacacttca tcacttccca taggtacggg cgaaccgcct aaaagctcta
     1501 ccattttgat agcggtttcg ctttgcatca cacggatctt ctttcccttc aaatcttccg
     1561 gcgtatggat cggaccgtct accatataaa agctgcgctg accagaatca taataggtca
     1621 atccggtaaa tcctaaattt ttggtcgatt ggtagattgg ctccattatt tcttcattat
     1681 ccatcacctt aaagaaatgt tcttcatcat tgaatagata aggaacggaa aaaatcgaat
     1741 aatctttaga gaaactttcc aacgctgagg cacttacttt tgtgaaatct actgctcctg
     1801 actgtgtcaa ttcgatcagt tcacgttctc cacccagttg tccatctggg aaatactgga
     1861 tctgtacgga accatctgtt ttttctttta ctaaacgccc aaattcattc attgcatcaa
     1921 ttactggctg ttgcttgcat aagcaaagcg caaggtaact atctcattta cgtctgcatc
     1981 tgcgacagtt gccacactag aattatttcc gcagccgctt agacctagca gacctaggct
     2041 aagcgaactt gctgcaaaca gcagtgatgt ttttttcata tgggtttcct ttcttttaac
     2101 aataagagac gagcaattct gtcaaacaaa gcaccgttaa cgactgtcca taaggcattg
     2161 ctgtgattcc gatattccga taaaaatcta aactgtcccc cattcctgtc ccgattgaaa
     2221 cgttttgaac ttctcctttt tcatcgattt gttcaagcaa tccttggata gcacgataag
     2281 cgacttcttt atattcttgt ggtaaataac gtttatggac cgctttcaaa ataccgtaag
     2341 caaatcctgc agttgcagaa gattctacgt aagaagaagg atcatccaac aacgtatgcc
     2401 ataaaccgct ttcagactga taagttttta atgcacgcac ttgtgcttca agtgtttcga
     2461 tcaataattt gcgcaatgca tcttctttcg ataattctaa gatttcaatg atctctggaa
     2521 tagcaatcgt caaccagcag tttccacgag cccaaaatgc ttcggcatag ttatgatttc
     2581 cttcaaacgt ccagccgtga taccacaatc ctgttcgttt atccatcaag tatttgatat
     2641 ggatcataaa ctggtaacga gcttcttcta aatattctgg gcgattcaac agtttcccga
     2701 tttttgccaa aggaagcact gtcatcatca atgtatcgtc ccacaactga ttcttatttt
     2761 ccggtccata tgtggcatgc tgtaacccgt cttcatttgt ccgaggcatg tcattcatga
     2821 cccattctgc ccattgttct aaataaggca aatagcgtga atctttcgtg tcttcataca
     2881 agtaagccat agtcaatagc ggtgccattg tattgacgtt tttgggcgga gcgccttctt
     2941 ccatacgatc ctcaaaccat tcattgacca cttgataagc gcgaggattt tttgtcatct
     3001 ggtaattttt ataaatacca tatagaccga caccttgtgg ccaattccac actgtccagc
     3061 ttttgtcatc tactttcagt ccatcaaagt ccaacaaaaa ttcaccatta tcgtctttga
     3121 tcgttgtcag gttttcaacg agcaggtcaa ttttttcttt cactaattct ttaggtacaa
     3181 aaatctcttc tttaataatc atctttacgc caccttttta aacgctttca ttttatatcc
     3241 ttaatatagc agtaataccg tccactataa atatcgcttt ttgaacaaaa actgtctaaa
     3301 attgacctaa atgttaaata acaaaagtaa ctttcagtta cttggaaatt aaaaaaagaa
     3361 cttggagaaa caacgttttc caagttcttt ttcatctatt ttcttatctt ttttctacgc
     3421 tttcttttgc ttatccgttg taagcatcaa tgatgatctg tttcaattca gagatcaatg
     3481 gttctttcgg attagctgtc gtacattggt cttcgtaagc taattcagcc atacggtcta
     3541 cggttgtatc tagtgtttct tgtgtgactc cttgagcttt tagactcatg ttgatgccga
     3601 cagattttcc taattcataa acagccgttg ctaaagcttc gactaattcc tctgttgttt
     3661 cgcctttcag acctaagaat tttgcgatat ctgcgtaatc tgtatctgca cggaaatagt
     3721 catatttagg gaacatcgca tgtttttgtg gatcttttgc attatagcgg ataatatgcg
     3781 gtaaaagaat cgcatttgtc cgtccatgag ggatcccgta ttccccgccg attttatgtg
     3841 cgactgagtg acaaatacct aggaatgcgt tagcaaacgc cataccagcc attgtagaag
     3901 cgttgtgcat tttttcacgt gattccatat caggtgtttt tactgatttt tctaaatagt
     3961 caaagactaa cttgattgct tgtaggctca atccacgtgt ataatctgaa gccatgactg
     4021 aaacatagga ttcgattgca tgtgttaaca catccatccc ggtatctgct gtgacagatg
     4081 ctggaactga catgacgaat tgaggatcga cgattgcgac atctggcgtc aatgcataat
     4141 ctgctaacgg atatttcaca tgtgtttcac tgtcagtgat gaccgcaaat ggcgttactt
     4201 cagaaccagt ccctgatgtt gtcgggatac atacgaattg tgttttttct ggtttgtcga
     4261 tcttatatgt ccgtttacgg atatccaaga atttttgttt tgcaccaaag aaagatgtgt
     4321 ctgggtgttc atagaacatc cacattccct tagctgcgtc cattgcagaa ccgcctccaa
     4381 gagcgattac tgtatctggc ttgaagtctg ccatcagttt cgttcctgcg tatacggtat
     4441 ttgtcgatgg gttcggttca acatctgaga agacttcgat cttcacatca tttttacgtc
     4501 gtgccaatac ttcacgaact gtatcgcagt aaccgaattg gaccatacca gggtcacaaa
     4561 cgatcatgac acgttcaacg ttttccattt tctctaagta caatagagag ttcttttcaa
     4621 agaagatttt tggcggtaat ttgaaccatt gcatgttatt tctccgtttc gctacagttt
     4681 tgacattgat taagtttaca gcagagacgt tacgagaaac agagttctta ccataagaac
     4741 cgcatcccaa agtcaatgac ggaatcattt cgttatagat atcaccgatg ccgccttctg
     4801 cagatggtgt attcactaaa atacgacaag ctttcattcg aagaccgaat ttgacatgca
     4861 actcttcgtt ttctgaatgg ataactgctg tatgtcctaa tcctccaaga ttcaacatac
     4921 cttcacacaa atcaaacgca tggtccgtgt tgttagcttt gatcatggca agaactggag
     4981 aaagtttttc tcttgatagc ggataatctg ggcctacgcc ttccaattca gcaatcagca
     5041 ttttcgttcc ttttggtaca ctgattcctg ccaattttgc gatttccatc gcagaatgtc
     5101 caacgattga tggattgacc gcatattttc cttcgttcat cactgcatct tctaattttt
     5161 gcaattcatc tggttttacg atataaactt ggtgtgcttg gaattctgct tttactgctg
     5221 catagatttc tttatccacg atcacagctt gttcagaagc acaaatcatt ccattatcaa
     5281 atgttttcga aacgatcaaa tcgttcactg cacgtttaat ttttgctgtt ttttcaatat
     5341 aagctggtac gttacctgga cctacaccta aagctggttt tcctgtagag taagcagact
     5401 tgaccattcc agctccacct gtcgctaaga cgatcgcgat acctggatgg ttcattagca
     5461 tagaagtcgc ttcgatcgaa ggatgttcga tccattggat acaattttca ggagctccgg
     5521 cagcgatagc agcatcacgt accactcgtg ctgcttctgc agaagatttt tgggcacttg
     5581 gatggaaagc aaaaacgatt ggatttcttg ttttcaacgc gatcattgat ttgaaaatcg
     5641 ttgtggaagt cggattcgtt gttggagtta ctccgcaaac cacaccgact ggttcagcaa
     5701 tttcaatcaa ccctttttgg acatcttcat tgatcacacc aactgtttta tcatgtttga
     5761 tattatgcca aatatattcg gacgcataca tatttttgat tgctttatct tcgtaaacac
     5821 cgcggcctgt ttcctctaca gccatcttcg ctaatggcat atgttgatcc aaagctgcca
     5881 ttgccatttc atgcacgata tggtctactt tttcttgatc aaagctttcc atttctttca
     5941 atgctgcatt tgctttttct gccaaatcat caatcattgc ttgtacgtca attgtcttcg
     6001 ttttttcttt ttctattact ttagccatca ttatcctcct aatgggtcat ggttatttgt
     6061 taaataattc acatgtatat catacaatat ttatttttct atgtaaacac ttttttgtga
     6121 tttttttcac agttattttt aaatataaaa ttgctttatt tacaggtgtt tgtatagaaa
     6181 aatatataaa gttatttatt attatttata tgacacaccc gattattcaa tattgagcaa
     6241 tatatcccat gctcttatct ctttcttttt tgaataaaaa atgaggtagg acatctttct
     6301 gccctacctc gtctttaaat aagctttgat acttattttt cttccttctc ttctgtctct
     6361 tccagcgttt ctgttgtttc agtttttact tcaactgtct cagctggttc ttctgttgtt
     6421 tcaacagtag tcgttgcatc atttgttacg acagtccctg gtttgaccgt tttgattgct
     6481 gcacgatcgt actcaaggaa gatcccttcg cagtctaaaa caactgtccg tttgtcattg
     6541 tcgatttctg aaacaactcc atgtaatccg ccgatcgtta cgactgaatc acctggtttc
     6601 atgctatcta acaagttttg acgttcttgc tgttgtttct tttgcgaacg tgacataaaa
     6661 aaccacattg ctaatagcgc gataaacatc acaagcattg gtaatgttcc catttttttc
     6721 acctcactaa ttctgtatct acaaaaaaca ctttagcaga aaaatgctga taacactagt
     6781 tttttactta atttttaaaa gattttcctt tagaagcttt ttgcgttttc tttattgaac
     6841 ccgtattctt caaaaaaggc ttctctaaat tcaagcaaat tatcatccat gattgcttga
     6901 cgtacttgtt tcattagatt caacaagaag tataaattgt ggtaagaagt caaacgaatc
     6961 ccaaatgttt catcacattt gatcaaatgg cggatatagg cacgtgtata gtttttacat
     7021 gtataacaat cacatttctc atcaattgga cggaaatcat gggcatattt cgcatttttc
     7081 actactagac ggccttgaga agtcatacat gtgccatttc gagcgattcg agtaggcaag
     7141 acacagtcaa acatgtctac tccacggata acaccatcga tcaatgaatc agcagcaccg
     7201 acacccatca agtaacgtgg tttgttttcg gggatcaacg gtgtagtgaa ttctaataca
     7261 cgattcattt ctgatttagg ttccccgaca gataacccgc cgattgagta accagggaaa
     7321 tccatcccga ctagatcttt cgcgctttga cgacgaagat cttcaaaccc tgctccttgg
     7381 atgattccaa ataatccttg acggtctgga ttagcatgag ctttcaatcc tcgttctgcc
     7441 catcgagacg tacgttctac tgattttttc acgtagtcgt aactttcatc gaatggtgga
     7501 cattcgtcaa aactcatcat gatatcagag cccaatttat tttggatgtt gattgctttt
     7561 tctggtgata aaaacatacg cgaaccgttc aagtgatttt tgaaatgcac gccttcttct
     7621 tcgatattcc gcatatcact taatgaaaaa acttgaaagc cgcctgaatc tgttaaaatc
     7681 ggttgatccc agttcataaa tttatgcagt ccgccagctt cttctactaa gtcttcacca
     7741 gggcgtagcc ataaatgata tgtattactt aaaataacgc cagcacccat ttctttcaac
     7801 tcttctgggg acattgtttt tactgtagct aatgttccta ctggcatgaa cattggtgtg
     7861 gggaaagttc cgtgtggcgt gatcaattcc cctaaacgcg cgccagtatg tttttctttc
     7921 ttgattaaac gatagcgaat ggcaggttgc gtcattcatt tccctacttt ctatataaaa
     7981 tatcttgaca actcccacca tcataatcgt ttcaagtgct ttttgcaagt tccctcggct
     8041 tccttctact ttatttaata ttcttttttt tatccttttt ttatgaataa caaaaaaaag
     8101 aaaaacatta tgacagaaat attttttgat acatggtata ttggtgttat taaagaaaag
     8161 gagggaaaaa tcatgcaatc ttctgcagaa ttaaaacgcc aagcaaaaga gagtctaaaa
     8221 ggacgttggg gacaagctgt tttactaaat ctaatcccta cattgattgg tattgctact
     8281 attcttctta ttatgattcc tattatcttg tttgccacca ccatatatga tggatcagcg
     8341 agcgtctcag aagcgacaac ttcaggaggc ggtagttcag gaggcggtgg cggaattatt
     8401 tccagcatca ttagcgcttt gttcatgagc ggtatttctt ggacttatct tgatatttta
     8461 cgtggacaaa aagatcagat cgaaccattc aaagatgcat ttcgtggatt caaaggtttg
     8521 tttatcggcg gagtgatcag cttagctgtg ctgatctcga tctttacaac attatggggt
     8581 ttgctattag ttattcctgg aatcattaaa agctattctt attctcaaag ctattttatt
     8641 tactacgata tcgtccaaga aacaggacaa acacctaaag tactggatac gatcactgca
     8701 agtcgaaaat tgatggatgg ttataaagga caacttttct ggttagatct ttcatttatc
     8761 ggctggcata tcttagcatt agcaactgct ggtattggtt atctatggct cagcccatac
     8821 atttctgcaa caaaagcagc attttatgaa caattgccaa aagatattta aaagagagtg
     8881 tgacttgtga ccgagggggt ggcaggagaa caccgtttat cgagaggcag taaaagaaac
     8941 attcagccca ttagcaaaaa ggaggtgtga caacattcat gtcacacctc ctttttgtat
     9001 agttccagta aaaagaaaat caacttagaa acagttaatc attcctcttc ttctgcttcc
     9061 ggtaatcgat ggaacttgat cagttcattc accgcgattg gtacgataga taaaatccct
     9121 accaagaacc agtgattcaa actgatgtgt actaggccga atagttcttg cgtaaatggc
     9181 agcaaagcaa cagccgttgt aatagctaat gccagtaacg ccatttctac caaagcttta
     9241 ttggtcgcta aattgacttt gaagatcgat tggctgctgc gtacattgaa cacatgcaga
     9301 atcgatgaat aagctaagat caagaatgca acagtttgac cgacatgctg acttgcgtct
     9361 acccatgggc tgatagtatc gacataacta cctaaataat aaccaaacaa ggttacgatg
     9421 gtgaagaccc ctgcatttat ggcaattttt tgcaataaac ctcgagcaaa gatcccttca
     9481 tttttcggaa tgggtggctg atccatgatg ccgtgttccg ctggctctct tcctaaagca
     9541 aatcctggaa gtccatcagc aactacattg acaaacagta gttgcacagc tgtaaatggt
     9601 gctccccatc ctaacagcat cgcaatcaaa acaataaaga tctccgaaat attacagctt
     9661 agcaaaaagt tgatagcttt acgaatattc tgataaaccg aacgtccttg actgacagcg
     9721 tctacgatcg tcgcaaagtt gtcatccgtc aaaatcatat ctgcggcact ttgcgccaca
     9781 tctgttcctg tgatccccat ggcacaccca acgttacttg ctttcaacgc tggtgcatca
     9841 ttcacaccat ccccagtcat cgctacgact gcacccgtac gttgccaaga tttgacgatc
     9901 cggatcttgt cttctggcgt tacccgagca tagacagaga gagctttgac acgcgcatct
     9961 aattcttcgt ctgataattg atgcagttct gatcctgaaa gtgcttctga cggatcattc
    10021 aagataccta attctttcgc tatggcactg gcagtcacca catgatctcc agtgatcatg
    10081 actgttttga tgccagcctt tttcgctcgc ttgattgctc ctttactttc tggacgtggt
    10141 ggatcgatca ttccgatcag accaatcaaa cgtaagtctt tttccagtgc ttcagaagaa
    10201 atatcttgcg gttcttcgtc ataagtagca taaccaacag caatcactct cagtgcccgt
    10261 ttaccaaagc gatcattaac gatcgccgct tgttctacat cgcctgaact aaagcgcgga
    10321 gcaagaacat cgaatgctcc tttggtgatt gagatgtatt tttcgcccat ttggtgaact
    10381 gttgtcatca tttttcgttc agaatcaaaa ggaagctcgt ttactcgtgg atatttttct
    10441 tctaattctg cctttgtatg ataattctct tcaacagccc ttacgatggc tacttctgtt
    10501 ggattacctt tgataacaag gtcgtcatct tcttgctcga ccgtcacatc tgtacatagt
    10561 gcagccattt tcaatatttc catcgcgctg ttcgtcattg aatcttcgat attcgttact
    10621 tcatcttctt tgctccatac ccggcggacg cgcatttcat tttgtgtcag cgttcctgtt
    10681 ttgtctgagc agataacatt agcggttcct agcgtttcaa ctgctggcaa ctgtcggatg
    10741 atcgcatgtt tcctcgccat cttctgcaca ccgtatgcaa gtgtcaacgt aacaataaca
    10801 gtcaaggttt ccggtacagc tgcaacagcc aaggaaatcg aagtcatgaa catctcaagg
    10861 agtggttctc cttgcagttc accaatcaca aatactaaag ccgcagccgc taaagcgatc
    10921 atactgattc gtttgcctag ctgattcaat cggacctgta aaggtgtttt ttgcatagta
    10981 ttttcgttca aaagatccgc gatcttaccc atttctgtag ccatgccaat ggcagtgacg
    11041 atcgctttcc cacgacctgc agcaaccgta catcctttaa ataccatatt aagttggtca
    11101 cctaatgact catcttcttc agcaataaat gctgcatctt tttcaactgc ttcactttct
    11161 ccagtcaacg ccgattcttc tacacgtaat tgtgatgctt cgatcaaacg ggcatcggca
    11221 gaaatagcat caccggactc taaaacaaga atatcaccca ctaccagttg ctctgactca
    11281 acagtggtca ctttgccgtc tcgtatgaca gttgccgttt gtttattcat atcttgcaag
    11341 gattccagtg ctttttctgc gtttccttct tggacaatcg ccaataccga gttgatcaca
    11401 acgatagcga tgatcagcag tccttcaaat aaatccccat gttctgtagc aaatgcggta
    11461 taaaaggaaa tcaccgctgc tacaagaagg atgatcgttg taaaatcgga caagcttcga
    11521 aagaattttt tgatcatcga ttctttcggc gcctcatcga atttattcaa tccctgcttt
    11581 tttcgccgtt catccacttc ttgtgaagac aacccagtat ctggatctgc ttctgttacc
    11641 ttctttactt cttctattgt ctgcttgtat tcttccatcc tattcctcct ttaaaatctt
    11701 cgtacaaaaa aaggtactta ttttactata acatcctttt tttatttact tcaaataaag
    11761 ggcttatcga ttatttgtta agaaagcgta aaatttaaag tgcttatatt tgcaaacgct
    11821 accaaaggat gctattatat agatgtaagg aaggaggata ccatcaaata gacagaagaa
    11881 tttctcgata gaacagtgta agcaaacaaa gttgttagaa gtacaggttt cacccagaca
    11941 tgcgtgtaga gtacaaacct ataaatagtg aatgcgtatg gatgtttgga tcacgaagta
    12001 tccgaaacaa gaagtaatgc gatacgtaac aattgaataa cgtaaacgtc tgaaaattga
    12061 ataggaacaa gagatgtttc aaatgttgaa gatgtaacga aaacaaaatt tttgtttaac
    12121 atgaagaaat tcaacaccgt gctttgcatg tatgatggaa agttacaatc atttctgttt
    12181 atttgaatcc ttccgaggta caagaaagcc gagcttatcg agagctcggc tttttctttg
    12241 tcataaaatc tttgtaaagc ttttttgtta ttcaaaacta gcggcttttc ataaccttat
    12301 ttcacgaaca tcgcatctcc aaaactaaag aaacgatagc gctcttcaat cgcatgctgg
    12361 taagcagata aagtcaattc tcttcctgca aaggcactaa ctaacatgac taaagtcgat
    12421 tttggcaaat ggaaattagt tgagaaagct tctaccactt tgaattcata gccaggcgta
    12481 atgaaaatat cagtccatcc gctgtctgct ttgatttctc cattaaattt cgttccaatc
    12541 gtttctaatg ttcgaattga agttgttccg acagccacga ttttcccgcc atttttacgt
    12601 acttggttca atcgctcagc cgcttcttcc gtcaaacggt agaattcact atgcatatgg
    12661 tgttcttcaa ttttatctac gctgacaggt cggaatgtac ctaaccctac atgtagtgtc
    12721 agatagacta attcaacacc ctttgcttcg atctcatcaa gtaattcttt cgtaaaatgc
    12781 aatccagctg ttggcgctgc agcagaacca ttttctttcg catagacagt ttgatagcga
    12841 tctgggtctt ccagacgttc tttgatatat ggaggcaatg gcatttctcc caatgattca
    12901 agaatctcta gaaaaattcc ttcatactca aagtcaatga tccgtccgcc gtgttccaat
    12961 tcttctttca ctactgcttg caatctgcca tcaccaaaag agattcttgt cccaactttg
    13021 gcacgtttcg caggtttgat caatgtctcc cacgtatctc cttgtgtatt cgttagaaga
    13081 aggacttcta aatgcgcacc agtatctggt ttttctccat ataaacgagc aggcagaact
    13141 cgtgtattat tcataactag cgcatcacca ggctgtaact cttcaatgat ttcatggaaa
    13201 tgtttatctt ccatctctcc agttttccga tccaaaacaa gtaaacgaga atgatcgcgg
    13261 tcttttaatg gtgtttgtgc gattaattct tccggcaagt caaaatcaaa atcttctgtt
    13321 gttaacatat cgtcactcac tttctatcat gtgcttcatc attctatcat gtttttcgtt
    13381 ctgttggcaa aatcagcact ttttcacttc tattttttgt tttcctaaat aaaaaaacta
    13441 ccctatttgt attttttcat tgttttaggc atactagtaa tagatagttc ttcattgaaa
    13501 atagaaaaag aggtggcacc atgattgaat ttcaaaatgt gtcaaaaatt tataaaggtg
    13561 gaaaagtcgc tgtagaggac gtcaatcttt catttgaaaa gggtgagttc atttgtttta
    13621 tcggtacgag cggaagcgga aaaacgacga cgatgcggat gatcaaccga atgactgatc
    13681 caacacaagg gaaaattttg attgatggaa aatccatcca agaaattgat ccagtggaat
    13741 tgcgacgtca gatcggctat gttatccaaa gtattggatt gatgcctcac atgacgatcc
    13801 gtgaaaatat cactcttgtc caaaaattgt tgaaagtcag tcaagaagaa cggaataaaa
    13861 cagctgaaaa aatgattgat cttgtggaat tgccccgtga gatgttagat cgctacccac
    13921 atgaattatc tggtggtcag cagcagcgta ttggagtcgt tcgtgccctt gcagcgaatc
    13981 aagatatcat cttgatggac gagccattcg gtgcgcttga tccaatcact cgtgattcct
    14041 tgcaagacct tgtcaaagat ttacaagagc gtttgggaaa aacgatcgta tttgtaaccc
    14101 atgacatgga tgaag
//
LOCUS       DACECY010000067        13919 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.071, whole genome shotgun sequence.
ACCESSION   DACECY010000067 DACECY010000000
VERSION     DACECY010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.071"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            133..507
                     /locus_tag="IWO38_002215"
     CDS             133..507
                     /locus_tag="IWO38_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0314290.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(621..1250)
                     /locus_tag="IWO38_002216"
     CDS             complement(621..1250)
                     /locus_tag="IWO38_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0314291.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1384..1456)
                     /locus_tag="IWO38_002217"
     tRNA            complement(1384..1456)
                     /locus_tag="IWO38_002217"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1421..1423),aa:Thr,seq:ggt)
     gene            1670..1743
                     /locus_tag="IWO38_002218"
     tRNA            1670..1743
                     /locus_tag="IWO38_002218"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1704..1706,aa:Asn,seq:gtt)
     gene            1698..1847
                     /locus_tag="IWO38_002219"
     CDS             1698..1847
                     /locus_tag="IWO38_002219"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314292.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2340..3668
                     /locus_tag="IWO38_002220"
     CDS             2340..3668
                     /locus_tag="IWO38_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ0314293.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3870..4703
                     /locus_tag="IWO38_002221"
     CDS             3870..4703
                     /locus_tag="IWO38_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ0314294.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4719..5456
                     /locus_tag="IWO38_002222"
     CDS             4719..5456
                     /locus_tag="IWO38_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ0314295.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5611..6579)
                     /locus_tag="IWO38_002223"
     CDS             complement(5611..6579)
                     /locus_tag="IWO38_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ0314296.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6811..7644
                     /locus_tag="IWO38_002224"
     CDS             6811..7644
                     /locus_tag="IWO38_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ0314297.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7678..8517
                     /locus_tag="IWO38_002225"
     CDS             7678..8517
                     /locus_tag="IWO38_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0314298.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8543..9103
                     /locus_tag="IWO38_002226"
     CDS             8543..9103
                     /locus_tag="IWO38_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0314299.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9302..11023
                     /locus_tag="IWO38_002227"
     CDS             9302..11023
                     /locus_tag="IWO38_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ0314300.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11188..12330
                     /locus_tag="IWO38_002228"
     CDS             11188..12330
                     /locus_tag="IWO38_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0314301.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12346..13557
                     /gene="thiI"
                     /locus_tag="IWO38_002229"
     CDS             12346..13557
                     /gene="thiI"
                     /locus_tag="IWO38_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ0314302.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4751 a         2298 c         2834 g         4036 t
ORIGIN      
        1 gtctacactc tgagatgtaa gaaatatcat aagatatttc ttacatcttt ttgttttgag
       61 caggacttta gataatttta ttaatatatg ctagcatgct tttgtaagaa aaagaaagag
      121 ggcgtattag ttatgtatct taaaattacg gaacaagcac aagaaagatt acaaaaatat
      181 attgatgaag gcgcaacagt tatattggat ttagatgatg gcgttgggga atattctaaa
      241 atgggtgttt gctctttaga tactagtttt cgtttacttc ttcttgataa agaacagaga
      301 aaaaatgatt ataaactcca attagatagt gatattgggg atgtttatat aaaagattat
      361 tcaaaaatgt acatggatga acatatgacc ctttctttag atccacgtct aggtgtattt
      421 actcttgaaa gcccaagtgg ttctttagac agtcatgttc aattagtaga tctacgacca
      481 gaaaaattct ctggaataaa acaataactt tctctctatt gtccataaaa ctagtctcaa
      541 tcaagctgta ttgaaaaaac aagacaacat tcttaccatg gtaaataaaa agacgacaca
      601 ttttgtcgtc tttttttaaa ttaactattt tcgataaaag atcgcatagc ttgtgccatt
      661 atttctggct tttctctatt caattcatgt tttccgtttt taataaagag tagccggcca
      721 ttcgttgaaa gtttatataa acgttttgct gctggtttat tcgcatgatc tttggtaccg
      781 cataaaatca atttaggaag tcgtgatgta cgaagctgtt ttttgcagtc taatgtttca
      841 agtgaacgaa gtagggtaag ggtttgtttt ttagatagac ctaatttttc aaatatctta
      901 gaaggtacgc agtaaaaata aatattctgg aaagtaagca ataagcgatt aggagactca
      961 aattgtggag cgactaaaac aacctttttt aaatatggag aaggagttgc aagatagttt
     1021 aaagcaataa cagcgcctaa agataatcca cataaaataa aaggttcctt tatctgattc
     1081 aattgtactt cgatgtattt ggttaagtcg tctaaagtag cagtgtctgt gctgtttaaa
     1141 tgattgaaca atgaaagact tttgcaagaa taactattgg gtagataaga gattacatct
     1201 ttccatgatt gttcgtcttg gcctagacca tgcaaaaaaa caattttcat attgctctcc
     1261 tttttatagt tatcatatag actttctttg tactattaaa atataaacat tcatttctta
     1321 cgtcataaaa taaaaaaaca gcgcagacac attgttaaac caacatgttg cactgtttaa
     1381 ggaagccact tgccgggctt gaaccggcga cctcttcctt accatggaag cgctctacca
     1441 gctgagctaa agcggcatta tcattaatac aaataatagg ataacgaata ttaatgtatt
     1501 tgtcaacaga attttttcga aaaaatgtgt ttctctaata ggaaaacaga ttttagaaaa
     1561 aataaaaata acaaaaaatg cgaacggttt ctttgataat ataataaaaa aattcaaaaa
     1621 aagtattgcg ttatttagaa gaaattgata ttatattcat gtacttgatt ccggcatagc
     1681 tcagttggta gtagcgcatg actgttaatc atgatgtcgt aggttcgagt cctactgccg
     1741 gagtaaataa agcttatgca aagaaaaaga aatccaatca cttgggtttc tttttctttt
     1801 attattatga tgaatcaagc tttctgtttt tgtcacgtat gttgtaatct ttacgaaaga
     1861 gtagagttgg ctactcataa tcaaccatac ttcccatcat cgctaaatac gaaataaatc
     1921 agcattccta caccaatcaa agcacaagct gcaccaagaa aagggatgac attcccaaag
     1981 ttatctgtat tcataagtat gccaccaccc acaaacaaaa agccgccagc aatcaggttt
     2041 ttcattttat tcatctcctt tcttacgtta tagtatttcc ttctttcaat gagtattttg
     2101 aaagctatct tattgtatga tgttcttcta ggcctcttct ttattataca ggccattttc
     2161 gttatctcat agaaatattc ctttttatct gaaaactatt gtataccgta tgacattttt
     2221 cttcatgaag ttgtaagtat attctcattc aaatgaaaat ttcatcatag aagagattgt
     2281 atttctaaat tcttaagagt aaaatacgaa gtataacttg gatttggaag gatgattgga
     2341 tgaaagctat tacgcctgat atgacaacaa aatttaacaa acagtttcaa gcaaatatca
     2401 aacagcaggc attgcagcgg gcagttgtga agaacggaat tactgcttct gctgaaaatg
     2461 tttcagcaca tgtggagaat acaccggtat tttctataga tttggctacg gggaaagtgg
     2521 ctaatcaaat gcaatcgggg cgttgctgga tgtttgctgc actgaacaca ttccgtcata
     2581 agatgctgaa tcaattccat ttaaaagagt ttgaattatc acaaaattac acttttttct
     2641 gggataaata tgaaaaggca aattatttct atgaaaacat cattgcaaca ggaaaagagg
     2701 caatcgatag ccggaaagta gctttcttac tcgctactcc tcaacaagat ggcggccaat
     2761 gggatatgat cgtttcatta ttccaaaaat atggcgttgt gcctaaatcg gtgatgccgg
     2821 aaagtgcgaa tagttcaaat tctcgagatt tgaataacta cttgaacaaa ttactgcgta
     2881 aagatgcagt agtgttacgg aaaatggttg ctcaaggaga aacattagac gctattgaag
     2941 aaaagaaaga agaaatgcta gaaagcattt ataacttctt agctatctca ttaggaacac
     3001 ctccaaaaga attcgatttt gagtaccgcg atgaagaaaa gaactatcat cttgaccgag
     3061 gactgacacc acaaattttt tatgataaat acatcggtgt aaaattagat gattatgtta
     3121 gcgtgatcaa tgcaccaaca aaagacaaac cattcaatcg ttcttataca gtagagatgc
     3181 taggaaatgt cgttggcggg aaagaagtca aatatctgaa tgttgatatg gaaactttca
     3241 aaaaattagc gattgctcag cttgaagaag gagaatctgt ttggtttggt tgtgatgtag
     3301 gtcaatcttc tactagaact agtggcatca tggccttaga tgcttattca atggatgatc
     3361 tatttgatac ggattttaca atgactaaag ctgaacgttt agattttgga gaaagtctga
     3421 tgacccatgc gatggtattg accggtgttg acttagtaga cgggcaatca acaaaatgga
     3481 aagtcgaaaa tagctggggc gaaaaagttg gaaagaatgg cttctttgtt atgagtgatg
     3541 cctggatgga cgaatatacg taccaaatcg ttgtaagaaa agatttattg acgaaagagc
     3601 aattaaatgc gttcaatgaa gaaccaattg ttctatctcc gtgggatcca atgggtgctt
     3661 tagcatagac gagtatttca atgaagagta tgtagtaaag ataaaaagag ctggcttaag
     3721 ccagctcttt ttatctttaa agtccattcc ctgtttttta tggtccctgt gctataattc
     3781 taaataaagg ataaggattg gaaataatcc ttttatcatt aaaaatatga tgacgaagta
     3841 gaataaaatt gggagtaggg gggaaagtca tggatatcca aactgtttta tctgatctga
     3901 aattaaatgg taaagtgatt ccagttgttg gtggcgatgt taatcagacc tatcgtataa
     3961 aaaccgaaca tcgtgcttat tttttaaaaa tccatccgaa tgtaaaaaaa ggattttttg
     4021 aagcagaagt agacggatta aaagaactgt cagcattcgt acgtgtaccg gatacgtata
     4081 tgctaggtga aacaagtgaa ggcgcttatt tgttaatgga atggatcgag ccgggaaaag
     4141 gggatcaacg agatcttgct gcagctttag ctaatctcca tcagcaaaca gcgccacaat
     4201 ttggttttcg taaagataac tatttgggta cacttgttca gaaaaattct tttgaagaag
     4261 attggtggac ttttttcttt aaagatcggc ttgaatccca gatttctttg gcagaagaaa
     4321 cgaatcgatg gaatgtacag cgtcaagaaa agtatctgcg atttaaagaa cgtgtattga
     4381 aatcggttga accaaaaaag atcactccac gcctcttgca cggagacctg tggagtggta
     4441 atgtgttctt tgaccagcaa gggcatccag tttttgtcga cccagctgtt tcttatggga
     4501 atcgggaaca agatattgca atgagccagt tattcggtgg tttccgaccg gaatttttag
     4561 atgcttatca aactatattt ccattagaaa agggatggaa agaccgactg ccgatttacc
     4621 agttatatta tcttttggca catttgaata tgtttggaga aagttacggt tcccaagtag
     4681 atcaattact agaaaatttt taagaggagg gcgtgttttt ggcaaaaaaa tataccaaaa
     4741 agcatgaagt ttcatattat gagtgtgaca tcaaccaaac gatgacattt ccttcgatgt
     4801 taggagtagt tatcaaaacg tctgaagatc aaagcgacca gttgggaaga ggcagtgaat
     4861 tcgtcaatag ctttggactt acttgggtca tcacgaatta ttccatggag attaatcgtc
     4921 tccctagagt cggtgaagta atctcagtaa cgactcaagc catggagtac aataagtttt
     4981 tctgttatcg gaacttctgg atccatgatg aagatgggaa agaattagtc aaaatagaat
     5041 ctgttttcgt attgatggat tttgtgaatc gaaaaatgag cagtgttaat gaagagatca
     5101 ttgctccttt tgagagtgag aagatcaaga aaatcaagcg tcaggaaaaa attgaaaaga
     5161 ttgattcggg cttcatgttg ccatatcgtg ttcgttttta tgatatcgat agtaatcagc
     5221 atgttaataa tgcgatgtat tttaattgga tcattgacgt attaggatat gactttttga
     5281 ctacgcatgt tccagaaaaa gtaattattc gatttgataa agaagtagag tacgggaatg
     5341 agatcgaaag ccattttgaa caaatcccta tcgaaaatgg tgtcaaaaca aaacatgaaa
     5401 tccgaatgca agaacagctg tattgtgaag caaatattat atggaaaaat aaataaaaaa
     5461 gaggttgtga cagaaggggg caacttcaag aaataagaag aaatttacga aaattgtttc
     5521 tcaaattttt gtaaattttc gacttagttt tcgaagaagc tatttttgtc acagcctctc
     5581 tttttaagtt tagctattag ataatgatgt ttagttttgg ataaacgaaa tcaattcttc
     5641 acccgacaaa ccatagttta cagcaatcaa tagttttaaa cgtgcttttt gactattaat
     5701 actgttacag aaaataatcc ccattttttc tagttctttt cccccgcctt cataatcata
     5761 aacaggctct gcgattccat tgaaacaacg agaaacaagt actacaggga ttttttggac
     5821 aagaagcttt tccaaagcag ctaaggcttg tggagggaga ttccccgcac ctaaagcttc
     5881 gattaccaaa ccgtctactt tcgtatgggc gatggcttcc aataagtccc cttgcatccc
     5941 agcatatgct ttaacgatcg ggatagtccc atctacagcg gaaatgtcca gtcgctttgt
     6001 ttctaaaagt tcctgcaaga ataaaattcg attcttagtt actaagccga tgggacccaa
     6061 tgtaggtgtt cggaaagtag caacatttgt ggtatgggtc tttgtcacgt agcgtgcaga
     6121 atggatctca tcgttcatga ccaccaaaac ccccttatct agcgcttctt cacaacttgc
     6181 gacacgaatc gcactttcaa aattataaag gccgtcactt ccaagttcat tacttgaacg
     6241 cattgcccca gtcaaaacaa ttggtaattt gttgccaatc gttgtatcaa gaaaaaaagc
     6301 agtttcttct aatgtatctg taccgtgtgt gatcaccact ccggatatac ctgatgcata
     6361 agcggacaag atacgttctt ttaataccaa catgtgagaa ggcatgatgt gtggtgaggg
     6421 aagattgaag atatcttcaa ctaataattc tacatgcttg gggattgtga tttctgcatc
     6481 taataatggg tttgccacat caggactaac accgcccgtg agcatgtttt ctttcatggc
     6541 gatcgttccg ccggtgtgta gtacaagtat ttttttcatt ttacagactt ccttacgaat
     6601 aattttgcat agccgttaaa tcttattgta tactatataa aagagttttg ctatcagtga
     6661 agaataacga atagcctaac ttatcataaa aaagctgacc ttattaaaat ggaaaagact
     6721 caaccttgac ctcctaatac aatggagtat ctggttgagt ttcttttaca agaaagcagc
     6781 caaggaaatc aatcacgaag gtggccggaa atgatcagaa tcggattaac atcgtttaaa
     6841 gaacatgaca aattaactgg taaaaagaat aacactcttt atgaatatgc agggtttcta
     6901 cccttagtgg aattagatac tgcctattat ggaattcccc gaaagtcctc agttgagaat
     6961 tggataagtg aagtacctga atcatttcgc tttgtagtca aagtctatca aggaatcagc
     7021 ggtcaaggag actggaagcc ttattatcag accgaagaag agatgattgc gcatttttta
     7081 gatgttatgt cgccaatggt agatgctgga aagatgtttt gctttttagt ccaattcccc
     7141 tcatcattca aatgtacgaa agaaaatgtc cagtacttgc gcaaacttag aaagtggttt
     7201 gagggttatc ctgtttctat agaattgcgg gattattctt ggtacagcga tgaatttctt
     7261 gaacgtacaa gaaactttat gtctagagcc gctttttctt tagccatcat cgatgagcca
     7321 cagttgctga atacaacgat tccttttgat ccatttgtca ccaatccgaa ctttgctctt
     7381 tttcgattcc atggtaggaa tgcgcaagga tggcaagcaa atgataaaga gtggcgtaaa
     7441 aaaagaacgt tatatcgtta ttcagaaaaa gaactgcaag aactagcaca tgaagtcaaa
     7501 gtagttgatt ctaagacaaa agaggtaggt gtgattttta ataataattc tggtggtgat
     7561 gcggctgaaa atgcattagc actgcaaaaa atattaaata tcaagccaga tggactcaat
     7621 ccaacccaaa tggatctgtt ttaagggctt attttattca agaaaggggc gaagtctatg
     7681 atcaaagcta ttttttttga tattgacgga acattggtca ccaatcgttc aaaagcgttg
     7741 gaatcaacaa aacaagcaat tgaatatgct cggaaaaacg gcattttatg cggtgtagca
     7801 acggggcgca gcccagtaaa gatcaaagaa atcattgatg aattggaatt agatatgtat
     7861 gtcgtgtata atggtcaact ggtttttact gcagatcgca cgattatcga ccaccctttt
     7921 gaacaaaaag tattggaaca tattgttgaa tttgcagatg agaaccatcg acaaatcgtt
     7981 tttggtgcga gaaatcggtt agatggatca acgacgatgc tattaggaca atcgattttt
     8041 atcaagcgac tagtcagctt tttaccgcgt aaattcccgg tacgtctgat gaaaaaaatc
     8101 ctccaggttt ttagccctca ccgacaaaag gatcgttatg aaacgttaga gatattaaaa
     8161 gaaccaattt atcaatgtat attgctcagt ccggagtcag aacagaaaaa gttagaacat
     8221 cgctttcccg agtgtacgtt tcagcgttca aatacgtata ctgtagatat catcccaaaa
     8281 ggtggttcaa aattactagg catccaagct ttcgcaaatg ctgtcgaaat tgaaatggaa
     8341 gaaattatgg cattcggcga tcattacaat gatatcgaga tgctgaaagg tgtaggaatt
     8401 ggagtggcca tggggaatgc tcagatcgaa gtaaaacatg ctgcagactt tgtgactcaa
     8461 tcaaatgaag aagatgggat atacgaagga ctgaaacact tcggtctgat ttattagtca
     8521 atgaaagaag gaaaaaatca gtatggaagc agagacacct tttcaaaaag tagaaacatt
     8581 ccacaaaact tttgatccaa gaagaccaga gatacccacg gcttttacag aaaatgaagc
     8641 tcatacacgt gctgcgttca aaatagaaga acttgttgaa tttgttgcag ctacttcacc
     8701 taataaagaa caactgcatg aagctgttat gctgctgcac gaagcattag ataaagcaga
     8761 agaaaaaata cttggtaaaa cacagtgggg aagtgatccg ctggttgaac aagtagatgc
     8821 actgacagat atcctttact ttacttatgg ttctttttca ttactaggaa tagtgcctga
     8881 tcgaatattc gaaatcgtcc atcaggcaaa tatggggaaa ttatttccag atggaaaacc
     8941 tcattatgac cctgtaacaa ataaagtgtt gaaacctgaa aactggcaga tggaatatgc
     9001 tccagaacct aaaatcgcgg cagaaatcca acgtcagatt caagaagcga ttgctaagaa
     9061 gaaatccgct gatataggca tgtgcacaaa tgaaaaagag tgatcagagt ttcactattt
     9121 ttgttatctt tatacgatgg aaaaacaaaa gattttcgtt taagttagtg aatgcatgaa
     9181 aatagtatgc tataatctag aatagatgtc ttttgcacga gagacgtatt atttggattt
     9241 atgccaagca gaacgccatc tgttgcataa aagatgcata ctgaaaacgg aggaaccata
     9301 aatgggaaat aatgttatca tcggaatcgt cattgcaatc attatagtcg ccattgtcgt
     9361 ctttttggtt agttacttga tgaagaaaaa aaaccaagat cgattggatg ccttagaaaa
     9421 acgcaaggaa gaattatttg acttgccagt gattgaagaa gtcgatgatg ttaaaaaaat
     9481 gcatctggtt gggcaaagcc aaaatacctt tcgtgaatgg agtcaaaaat ggaatgacat
     9541 atctacttcc tcgttcgctg aactagaaag tcaaattttc gaagtggaaa acctcaatga
     9601 aagttttcgg ttcttaaaag gaaaaaaagc agttgttgaa gcagaagcta caatggacca
     9661 aatggagaaa caagtgacgg aaatccgtga aggtctaaag gaattgcggg aaacggaaga
     9721 acgcaactca ctagctgttc agcaggcact tgacgtctat gaagaactga aagaaaaagt
     9781 cagagaagac gagcatgaat acggaccagc tctttttgaa ttgcaaaaac agatcaaagt
     9841 gatcgaaaaa gaatttacgc agtttgtgac tttaaacact tctggagacc ctgttgaagc
     9901 ccgcgaggtt ttggataaag cagaaaaaca tacgtatgaa gtggaagctc tgatgaaaaa
     9961 aattccaccg ctgtatgagg agctccatac cactttccct gaacaacttg aagaaatcag
    10021 tgatacttac gagaaattga tggaagaaca atatgtattc ccagaagaag atcttgctga
    10081 agatatcgca aaagtaagtc gaagaattga aaattcatta gccaatcttg aaaaaacaga
    10141 agttgaaacg gtcgaatttg aaaaccgtga aacagctgat ttgattgatt cgttatatga
    10201 tatcttagaa cgggaaatgg aagctcagag atatgtaaaa actaaccaat caacgattgc
    10261 agagtatatc aaacatacaa caaagaataa ccgtcaatta ttgattgagc tggatcacac
    10321 atctcaaagc tatacattga accacaatga aattggccgt gtacgaggtt tccaaaccga
    10381 agtggaagaa atggaacgtc aaaatgaaca gatgattcca cagatccgtc aacacgaaat
    10441 tccttattca gaagtgcgca cgttctataa aacagtcttc aaagtcttag aagatatcga
    10501 aaagcaacaa gtagagatcg acgactcatt acatgaattg agaaaaggcg aaaaagaagc
    10561 gcaagaaaag atcgatacat tcgaattcaa attgcgcagt ttgaaacgtt ttgtagaaaa
    10621 acaacgtttg cctggcttgc cgaatgatta cctagaattc ttttttgttg ctactgaccg
    10681 tattgaagaa ctaagtgttg tcttgaataa aattcgagta aacatggaag aggtcaatcg
    10741 tttagttgct ctatgtgaag aagatctaga attattagat aagaagacac atgatttagt
    10801 agatgcagca gcattaacgg agcaaatgct ccaatatgca aatcgttatc gtcatacaca
    10861 cgaagaagtg agagaagcaa ttgataaaag ctactattta ttcaataaag aatatcacta
    10921 tcaagaagca ttagacgaaa tcggtacagc attagagcga gtagaacctg gtgctttcaa
    10981 acgcatcgaa gatttttatt ttaatcatcc agacttagta taaagcaaaa aagttagtaa
    11041 accagcaaga gcaagacgga ttattgtctt gttcttgtct ttttttgtat atttgaaaat
    11101 tgaaacaagt gaaaatttca cctgctcttg aaagcacctt ggacaaccgt tataatagaa
    11161 aagaatttca aaaaagaggg gtttcttatg atatattttg ataacagtgc aacaacccca
    11221 atctttccgc aaagcttaga tgcatatgta aaaaccagtc agcggattat tgggaatcca
    11281 tcaagcttgc atgatttagg caatcaagct aaccggttgt tgcagcaagc aagaaagcag
    11341 atcgctgatt tgatccatgt agaaccagaa gaaatctatt ttacttcagg cggaacagaa
    11401 ggagataact gggtattaaa aggaacgatg atcgaaaaaa gagaatatgg gaatcatatg
    11461 atcatttcag gagtcgaaca tccggccgtc agcgagacag cagaacaatt aaaagaatta
    11521 ggaatcgaag taagtatcgc cccagtggat aaacgcggat ttgtacgtgt cgacgaattg
    11581 aaagaattaa taagaaaaga gacagtatta gtctctgtta tggctgtgaa taatgaagta
    11641 ggatcgattc agcccatcca ggaaatcagt gatcttttgg atgattttcc taaaattcat
    11701 ttccatgtag atgctgttca ggcgattgga aaagtaccat tctcgcaatg gttgacagaa
    11761 agagttgatt ttgcaacatt ttcagctcat aaattccatg gcccacgggg aactggtttc
    11821 atttattgga aaaagggtcg tcgtttagca ccattactga caggtggagg gcaagaaaga
    11881 aatcagcgaa gtgggacaga aaatgttcca ggtattgtgg ctatggttaa agctttacgt
    11941 ttatcattag agataaagga acaaaaacct gagcagacac aacgcttaaa agtagctcta
    12001 atcaatgcgt tgaagacgta tgagaaagtg acgatttttt cagaaggaac tgactatgca
    12061 ccgcatattt tatgttttgc tctaaaagga attcgcggcg aagttctcgt ccatgcctta
    12121 gaagaaaaac aaatatttgt ttctactact agtgcctgct cctcacgtaa acatatggca
    12181 agcagtacat tgcacgcaat gcatgtacca aatgatctgg ctacatctgc tgttcgaatc
    12241 agcttagatg aaaacaattc gcttgcagaa gtcgagcaat ttatgattat tttcaagcag
    12301 ctttataaga aattttctgc aatcaattaa aagaggagtg ttcttgtgaa atacagtgaa
    12361 attatggtcc gttatgggga actttcgaca aaaggaaaga atcgaaaaag ctttatcatg
    12421 caactagcac aaaatgtcag acaagctttg aaagactttc cagaaatcaa aatccatgct
    12481 gatcgggacc ggatgcatct tttattgaat ggcgcagacg ggcagctagt tattccaaaa
    12541 ctagcaaaaa tatttgggat ccaaaatttc tctccaagta ttcgtgtaga aaaagatgtc
    12601 gaagttttga aacaagctgt tcaagacatc atgaaagaaa tttatactgg gaaagaaaca
    12661 tttaaaatta tcgctaaaag aagcgatcac caatttgaat tgacaagtaa tgaattgaat
    12721 caaacattag gtaatgctgt ttttgatatt ttcccgcata tccaagttca aatgcgtcaa
    12781 ccagatattc cattacgtgt agagattcgg cgtgatggcg cttatctatc gtatgaaacg
    12841 atcaagggag ctggaggatt gcccgtagga acaagcggaa aaggaatgct tatgctttct
    12901 ggggggattg attctcctgt agcagggtac tttgccatga agcggggagt ggaaattgaa
    12961 gctgttcatt ttgctagtcc gccgtataca agtgaacaag cactgcaaaa agcaaaagat
    13021 ctaacagcca aactagcacc ttatgtggga acgatccatt ttatcgaagt gccatttacc
    13081 gagattcaag aagaaatcaa aaagtcagtg ccacaaggat attggatgac aattactcgt
    13141 cgaatgatgt tgcgtttgac cgatgaaatt cgccgtattc gtcatggttt agtcattttg
    13201 aatggtgaat cattaggtca agtggcttca caaactttgc aaagtatggt ggctatcaat
    13261 gaagtaacga atacgccaat cattcggcct gtagcgacaa tggataaact tgaaatcatt
    13321 gaagtagcag aacagatcga tacatttaat ctttctatcc agccttttga agattgctgt
    13381 acgatttttg ctcctccaca gccaaaaaca cgccctcaac tggaaaaggt ccatcaatat
    13441 gaagagcgtt tagcaataga tgaaatgatc caacgagcac tagctggctt gaagatggaa
    13501 gagatacaag cagaatcgtc taatgaaaaa actgaagaat tttcagaatt gctataagag
    13561 gttatgaaaa tagtgttttg accagagtat tagagaaaca aaacgaaaaa atagctccat
    13621 gtattttacc gtttttgaac taatgtcgaa gattagctga ttgaatacca tttatcaaga
    13681 agttatggaa aagccggttc ttttttacta actgaaaaat aaaattttat ttagagatca
    13741 agctacagtt gataaatgat ataaaaaaga gaatttccca tgaaatagcg aggttgtgac
    13801 agaagtgggc agcttcaaga aataagaagg aatttacgaa aattgctttt caaatttttg
    13861 tgaaatttcg gcttattttt cgaagaagtt acttctggaa caccgtgtat cagaggttg
//
LOCUS       DACECY010000068        13894 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.072, whole genome shotgun sequence.
ACCESSION   DACECY010000068 DACECY010000000
VERSION     DACECY010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.072"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(186..1553)
                     /gene="nhaC"
                     /locus_tag="IWO38_002230"
     CDS             complement(186..1553)
                     /gene="nhaC"
                     /locus_tag="IWO38_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ0314303.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1662..3083)
                     /locus_tag="IWO38_002231"
     CDS             complement(1662..3083)
                     /locus_tag="IWO38_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0314304.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3326..5203)
                     /gene="tdc"
                     /locus_tag="IWO38_002232"
     CDS             complement(3326..5203)
                     /gene="tdc"
                     /locus_tag="IWO38_002232"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ0314305.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5494..6750)
                     /locus_tag="IWO38_002233"
     CDS             complement(5494..6750)
                     /locus_tag="IWO38_002233"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ0314306.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            7330..8631
                     /locus_tag="IWO38_002234"
     CDS             7330..8631
                     /locus_tag="IWO38_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ0314307.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8700..9299)
                     /locus_tag="IWO38_002235"
     CDS             complement(8700..9299)
                     /locus_tag="IWO38_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0314308.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9553..10434
                     /locus_tag="IWO38_002236"
     CDS             9553..10434
                     /locus_tag="IWO38_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0314309.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10447..12057
                     /locus_tag="IWO38_002237"
     CDS             10447..12057
                     /locus_tag="IWO38_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAQ0314310.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(12134..12793)
                     /locus_tag="IWO38_002238"
     CDS             complement(12134..12793)
                     /locus_tag="IWO38_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0314311.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIESGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(13076..13741)
                     /locus_tag="IWO38_002239"
     CDS             complement(13076..13741)
                     /locus_tag="IWO38_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314312.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         4069 a         2811 c         2551 g         4463 t
ORIGIN      
        1 aagagcgtgg gacaaaagta aagaacactt ttgtctcacg ctctaaacac gtataaacgg
       61 cggaagcaga agcaactcct tcggaaataa gctgaaattc acaaaaattt aaaaagcaat
      121 tttcttgagt ttcttcttat ttcttggagt taaacgcttc tgtcccagcc tcttttttcc
      181 ttccttcaat tttttacacg tcgacgattc aataaaaaac cgcctaatat tgtaaagaca
      241 ggtgcaagaa tcgagaaaaa ggcgaatggc aagtaatgca acgcgctaac tttcatcgtt
      301 cccataatga atgtgccgct gactccccac ggtacgagag aattgacggt cgcacctgaa
      361 tccgctagtg ttcgtgtcaa ataggttttt gataagccaa cgtcatcaaa agaacgcttg
      421 aatgtttctc ctggaagaat gatggacaga tactgttctc caacgatcag attgattccg
      481 atacagctca aggcagtaaa cccgactaaa cgagaaggtc ggtccatctt ttcttttaat
      541 tcttgaacga tggtatcaac gatcagatat ttgatcagta acccacctaa tgccaatgca
      601 agtaaaatca aactagcaga acctaacata ctcatgatcc cgcctcgaga aagcagttca
      661 tctagctttg gatcaggtgt atttgcgaca taaccattca tcaagatgtc tgctgctttt
      721 ccgaccgtca aacttttctc atggataaaa gaaagaacga gagcagctgt cgatcccgct
      781 agtaatgtag ggatagccgg aatttttttg attgcccccc caaataaaat aactagtgga
      841 atcaatgtcc aaggagagat ccaaaagctc gctttcatgt tttggatcat ttcattgata
      901 ctagctagat cggctgaatg actgttatgt cctaatatcg tataaaaaat caacgaaagg
      961 acgaatgctg ggaaatccgt ataacgcata ccagcgatat gttcgaatag attaactcct
     1021 ccaatacccg ctgccaagtt ggtcgtatct gataaaggcg agcagttatt ccctaaaaag
     1081 gcaccggaaa cgactgcgcc tgtagtcatt gcatcattga atccaagtaa atgaccgatc
     1141 cccaaaaaag caattccaat cgtcgaaatc gttgtaaatg aactgccgac agtcactccg
     1201 acaattgcac aaacaagaaa tgctgtcggt aaaaagaaac gcacagaaac aagtttaaat
     1261 ccataaacca taatcgtagg gatggttcca gataaaatcc atgaagcaac taagacacca
     1321 atcaaaagaa aaatcaggat tggaatgatc cctggagtga ttcctttgat gatcccgtca
     1381 tggatctcat cccatgtgaa ctttttaaat ctgccataaa acaacaaaca catgaaagct
     1441 gcaaggatcg ggatatgtgg cgagagctgg aatccgatga tcagtactcc taggagtgcc
     1501 aacaacacga tcaatacagc gatactttct tttaaagaaa tatttttatt catcgtcgat
     1561 ctcctatttc aaatgaaaag gccctgccat gaaaaaacta ttcacttggc aggacctcct
     1621 actaaatcag cgacaaaacg tcggctgaaa gacttatttt tttatttttc ttctaaactt
     1681 ttttctacct caatccccca agaatctttc ttgaagtgat tgatgatcaa tggaataacg
     1741 aacatgacca ctgtgatagc tgctactaat aatgcatcac tcaatgtcgc tgtacctaat
     1801 gtagcaaata cgacaacgat gatagagaag atcagcatgc agctgactac ccaagctaat
     1861 ccgtttccgt ttttaccaat tcggtaagga cgttcgaatt ctggatgttt cttacgcatt
     1921 ttaagcaatg aaacagcaat caaaatgtac acgatacaat aaataacagt tgtagcattt
     1981 gtaagtgtca agaatacagc acttacgtca tccattacac cataaagcaa agcaaataat
     2041 gaaataacga ttgattgtgt tagaacgaca tttctagata cgccgtattt gttttcttta
     2101 tggaatccga attttggtgg aaggaatcct tcacgagcta cttggatgat tgtttttgat
     2161 ggtccagtaa cccaagctga taattgtaaa agcacaccaa taaataccat gaagctgaag
     2221 atatttccga tcacttctgg taagcctagg atttgacagt caatcaagat aggctgcgtg
     2281 atatttgcta attccatttt tccatctggt acgacatttg ctacaagcat cgcattgatg
     2341 acattcaata aaactaatcc gatcaaagcg ataaatacgc cttttgtata attttttgtt
     2401 gcgtctttca aacgaggcat gtaaacagaa ctcatttcga tcccaacaaa aataaatgtg
     2461 ataccagcta agtatttcaa tgaatcaata tgttccaagt cagggaaagc ttttgaccat
     2521 gaaaagtcac ctagatagcc gtttgagact agtccgactt tgaaggctgc caaaacacct
     2581 aaaacgaaca tgatgactac tgggatatat acccctagcc atacaccgat gttcccgccg
     2641 acttttgcca tatcgaattt caagttcaag atcgtgatga tccagtagat aactaaaata
     2701 caacctaaaa cgaaccagtg attattgcca agttctacat taccaaatgt gtttcctaac
     2761 aatggtccta aagtagaagc gaccattacc atacctggga acatttgcac ccataatagc
     2821 catgcaacga caaatcccca tttgctgccc agtgctgttt ttacccataa ctgaggaccg
     2881 ccttcttgag gaagcatcgt ggaaagctca ccggaaacca ttgatatcgg accggcaaaa
     2941 aacaaaactg caaaaattga ataaaaaatc aatgtccaac cgactgcggc taacgtagga
     3001 atactccgaa ccgttgcaca cagtgccatt gtcagaccga taaaggtccc aagacccatt
     3061 ttacttactt cagatttact caccgttgac actctccttg attttttgga taaaataacg
     3121 gtgtctttga agtagtttca gcaataaacg aaacaattca gcgaatattt gaggggattc
     3181 tttgagttta ttgcttgaag ggtttcttca aagacaacct caaaacaaag ggatcgaaga
     3241 gcttaggtct ccccttgctc ttcagatcca aacatgcgtc agaaacagtt agtattcaag
     3301 ctgttttcta acatgattac cttgcttatt ttgcttcgct tgccaataat tggtctgcgt
     3361 agattttttc caatttttct tgaagagctg ctttgatttt ttcagcatat tcttcaaagt
     3421 gttcttcttt gttcatgtat ggtgtcatta cgctggcacg caatacagtt acttttccgg
     3481 cacggttcca ttcttcatct gagaatccta gttggttcac aaattgtaat gggctgttgc
     3541 catagtctgg gatagcaaag tcagtatgtg aagttaagaa ctcattaccg taaatgcttc
     3601 ctttcacgta tgaagaataa tcatatacgt catggttcaa tttattcatt gcaactaagt
     3661 catcgttgcc tttttctttg aatacatagt ctaccatatt gaaatctgga taagttaatg
     3721 gatgaacttc gatttctttg tctcctactt taaatgacaa gtcattcaag aagttgtaga
     3781 aacgatgtgc accttcgatt gatgcaccca ttaatttacc atatcctgtg acattcaaag
     3841 gtaagacatg gtgtgctgcc caaacgctgg ctgctgttgc ccctgctttt gaaccttcca
     3901 agatataagc cccaagtaat gctgggatat ctgcgccttt ttcaaataca tatgtcgcaa
     3961 agtaagagat aacgtctctc atgcggatat ctttgatgac gataccaccg gcagaatatg
     4021 gaacatagcc cattttatgt gggtcaatcg ttacagattc tgcttcttcg attgctttat
     4081 acgcactgtg tacttcttct aagatataat ctttgttttc tgtaaagaca ttgtatttgt
     4141 aatgaacatc ttttaaatct tcaaatggaa tgaagttgtt gtcttcgtct aagaaaatcg
     4201 cacggccgta tccgccataa gcagcatcta cgtgtaagta gaagtaaata ccgtcttttt
     4261 ctaatacgcg acgcaatgca acgattttat cgataccgtc gattgctcct tcttctgttg
     4321 atcctacaac accgactaca cctaggattg gtgttttttc agctgctaga ccacgaacga
     4381 ttttttcaag ttcgttgatg tccatgcgat agttgtggtc aactggcact ggaatcactt
     4441 ggtctaaacc gataccgatg atgtcggcag cttttagcca tgaatagtgt ttagtttgtg
     4501 gaaccaacca ttttcctaat ttttctaaat gttttccgct acgtgctgag tgagctttga
     4561 tttcgtcgat tttttctggg acgctgtcta acagattcat gatttcttct gttgacaagt
     4621 tcattaattc ccaatcgctt tttccagcaa ctagttcagg tgtcacttct ttcattgcaa
     4681 gcggcaatga tttgatgttg cgggcatacc aaagtccttc taagtttgct aaagaaccat
     4741 cagcaacgat gtggccccag ccatctttat agctcattaa ttttgcaaat tccattccta
     4801 cttcttcttc catttggctt gttgcaggag aagattcata ggcaacgtta ttaccattcc
     4861 atagcatagc aaaattgtac gctagtaaag atggcatcaa tgtttcagag ttcatatgac
     4921 cccagtaacg tccggcatta tgccaaggaa cggaatgggt acgcatccgc gctgaaattt
     4981 ctgatagaac atcctttgtt ttattcacag tgtgttcaaa actagcactg cttttttctt
     5041 ctggcgtaat aataggcatg tcttgaggca tgtagttttg acgccagcct aaatgttcat
     5101 ccaccaattc attcaacaaa gctttataga tttggccgtt ctcagctttg tccccaatga
     5161 atagggcatt taggtttaga tctttcgaca atgattcact catagtgaaa ccctcctaaa
     5221 aaaatattta aattttgtcg agccctacat tacacctgtt cttctattct gtaaactaag
     5281 ttttggcctg atttttaatc atattttaat atgtatcaat tatccgaatt gttcacactt
     5341 ttgaaataat taaaaaaaac gagggaattt acaaatgtaa actccctcgc aaagattgta
     5401 ttaacgacaa ccagcgttct attttaggat gggtttcaaa attggaatgt tgtaatagta
     5461 tttccctttt tcaaggtaaa aacttactaa attctaatca attactttgg ccaaaaagta
     5521 atttttctta ccttttcgaa taaccacaaa cttaccatcg aattcatcgg atggatttat
     5581 gacaaaattt aaatcggtaa cgcggtcacc gttaatactt atagctccgt ttgaaacatc
     5641 ttcacgtgct tgacgtttag atggttcaat ttttgtggaa acaagcagtt ccacgatatt
     5701 ttcaggtgtg cttgaaattt caacgttcgg cattttaccg aatccttgcg cgatttcttc
     5761 cgcatttagt tctttaatat ttccagagaa caatgcttgc gttatttttt gcgcttcttt
     5821 caagtcttct tcactatgca caaatcttgt aacttcttct gccaaacggc gttgtgcttc
     5881 acgtttctca ggttctgtct ctactttttt cgctaagtct tcgatttctt cttgagacaa
     5941 gaaagtaaag aatttcaagt atttcactac atcgcgatca tcttgattca accagaattg
     6001 gtagaactca aatggtgaag ttttcttcgg atcaagccaa acggcaccgc ctgcagtttt
     6061 tccgaatttc gttccgtcag ccttcagcat caatgggatc gttaaaccga acgcttctgc
     6121 ttctggtcct tctaattttc ggattaggtc taatccagct gtgatatttc cccattggtc
     6181 tgctccaccg atttgcaatt gcacatcgta tgttttatgc agatgcaaaa agtcgataga
     6241 ttgcaggatt tggtaagtaa actctgtaaa tgagattcct acttccaaac ggctggcaac
     6301 gatatctttt gctaacatcg tgttgatgtt gaagttttta ccgtagtctc tcaaaaattc
     6361 aagtaaagag atttttgaca accagtcgta gttgttcaca aatgtgatat ttgcatcttt
     6421 accaaacaat tttctcattt gattggaaag tgcgtccacg ttatgttgca cttgttccat
     6481 cgtttgcaac acacgttctg ttttgcggcc gcttgggtcg ccgatcgttc ctgttccgcc
     6541 gccaatcaga atatatggat gatggcctgc caattggaat cttttcatca tcataaatgg
     6601 aatcaaatgt ccgatatgca tcgaatcacc agttggatca acgccgcaat aaagtgaaat
     6661 gctcttttct tcaacgagct cgcgaagtct ttcttcgttt gtttgctgat taatggcgtc
     6721 gcgccaagtt aattcatcaa taatattcat tttactgacc tccgtgtagt ttttttatga
     6781 aagcaggaac ctgctagcat gtgtaattaa ttatttcttc aggataggat aggaaataaa
     6841 aaaagtccct tggcatataa gccaagggac gaaatctcgc ggtaccaccc aatttgtatg
     6901 tctagatatc caacatacct ctcgaacatt taacgctgtc gcgtggcaat cactgccaaa
     6961 gactcctgaa atgtaattcg ctttaaagca tgtactggtt tgcagcgacc accagctttc
     7021 tgaaacattg gctttaacaa ctacttcgtt tcgatcttcg tcttgttatt attaatatgt
     7081 gtctatcata gagcgcaact ttttctttgt caacagaaaa aggatatttt ttttaaattc
     7141 ctcatttttt cttttttgaa tccgctctaa ttccgaacat tttattttca attattgtta
     7201 aaagaaataa ctttttctgt attttttctg caatttatcg agaagtcact ttactttgcc
     7261 tcttattctt tcttcgttac aataaggaat gggacaagta aaaaaatgaa aaagcaaggg
     7321 acgaggtata tggaaaaaaa gcaaaaaatt attttaggag ccacttctgc tttaactatc
     7381 gttattttaa ttctaagttt gttttttgtc atccgatcat ctacaaaaaa agaaccaatc
     7441 attcctgaaa aaaaaataac agaaaaagca aaagaaccga cagatgataa gcaacaacta
     7501 gttactcaag caaaaaatct ggctcaaagc tacgattatg accaagcgat tgcgttattg
     7561 gaaaaagata gtaagaacgg atctactgaa acacaacagc ttcttcaaac actaaaaaag
     7621 gagaaagaac aactgatcga ctggtctgat cctactcaga tctctcatgt ctttttccac
     7681 agcttgatcg tggacccgca aaaagccttc cactctcaac aggcacaagg atacaaagat
     7741 tatatggtga ctgttgaaga attcaaccgt tcgatcgaac aactttatca aaatgatttt
     7801 gttttagtta atttgaatga tttgatccaa aaagacgaac aaggaaatct ttcctttaac
     7861 gggcttgcgc tacctgaagg aaaaaaacca ttggttcttt cacaagatga tgtcagctat
     7921 tatgaatata tggataattc cggttttcct agccgattgg tcgtatcaaa taacaatgaa
     7981 atcaaaaatg tctataaaga gaaagataaa gaagaaatcg gtgactttga catggttcct
     8041 ctgatcgatg cctttgtcaa gaaacatcct gatttttctt atcgaggcgc aaaaggtacc
     8101 ttggccctga caggttacaa cggagtttta ggttatcgaa catcaaaatc tcaatatgga
     8161 gacaatgaaa aaacaaaaaa agagattaaa gaagctaaaa aagtggcgga acagttgaag
     8221 aaagacggct ggagctttgc ttcccataca tggggtcact tgaatatgac tcaagcttca
     8281 ctagaagatg taaaaaaaga caatgaattg tggcagcaag aagttgcacc tatcctagga
     8341 aaaacaaata ttctgattta tccatttggt gcagacatca gcgactggca gccttattca
     8401 gcagaaaatc aaaaatttgc ttacttgaag agtcaaggtt tcgatatttt ctgtaatgtc
     8461 gatgcttcta caccagcttg gggacaatta ggtacggatt tctatcgaaa tgctcggatc
     8521 aatatcgatg gcattcgttt tcaatctgat ttagacggaa aaaatcctat tcttgatcca
     8581 ttcatcaatg tccatgatgt ctatgacaaa gaagcacgcg aatctgcttg attttacgtc
     8641 atttatgaac caaaaaagcc gaactgacat tttatcgtca gtttcggctt ttttatgctt
     8701 tatttcttct tcggtgcgat tcccttgatc aggacttctg tccattctgc tgtatatgga
     8761 agcagttttg cgtatatctc cggcttagaa attccttcta ccattgcttg aaaatagagg
     8821 atcaagaact catccgaata tttcaaatcc acttttcctt ctttccgccc tcgatggaaa
     8881 agatcaagca tgatactgaa actttcttgc gtatacgcat ccatcgcgtc agaaaattct
     8941 tttttttctt tttttgtata gtatgtcatc aaatcgatat aaaattgttt gttcactcga
     9001 ttgagataac ggattttgtt ttcactcatg gcaatcagtg tttcttcaaa tggaagattt
     9061 tccgcaatca acgctttcgc ttcttgccct aaatcattca taaatgcaaa aaatacttca
     9121 taaatcagcc cttccttgct gccaaaatat ttaaaaatcg tcgctttact tacctgagcg
     9181 ttctcagcta ccttatccat tgtcactcca gcggttccta tgtctgtgtt catcaagtca
     9241 aaagctgctt gtaaaatctc tttctttttc tcttgtgctc tcttttcaaa accgttcatt
     9301 tgtagagcct cccgtaattt taaactttta taatatatat agttcaaatt tattctgagg
     9361 tttattataa ctatctattg agaaagtatc aagctagcaa ttccttgttt atgaattcat
     9421 tgacagaaat ttataaaaag aatattatgt actaaaataa taaaaaaagt tttataaaaa
     9481 ggtataaaac ctttttatta aactatttaa tcttgtttag tatatatttt tagaaaaagg
     9541 agtggggttt ttatggcaga aatcgtcaaa gtcacacatt tgaaaaagag ctttggtcgt
     9601 ttcgaagctt taaaagatgt ctctttcacc gtacattcag gtgaagtcgt tggatttatc
     9661 ggaccaaatg gttctggaaa atccacgacg attcggacac ttttagggat tataaaaaaa
     9721 gacggcggaa cggcaacgat ttttgggcaa gatgtatggg aagatagttt atctatccat
     9781 gaacgtcttt cttatgttcc aggcgatatc tctttgtggg gaaatttgac tggaggagcg
     9841 atcatcgatc tcttcatgaa gcttcatgga aaagggagtg ctcaaaaaaa agatgaactg
     9901 attcaacgtt tctctcttga tcctaagaaa aaagcaaaaa actattcaaa aggaaatcgc
     9961 caaaaagtgg gcttgattgc tgctttggct gttgattcgg atctctattt atttgatgag
    10021 cctacttctg gtttagatcc tttgatggaa acgattttcc aagaagaagt tgaaaaattg
    10081 aaagaacagg gcaaatccat cctactttct tctcatattc tcagtgaagt cgagcgacta
    10141 gcggataaag tcgtgatcat acgcgaagga acactcgatg aagaaggaac actcgatgaa
    10201 ttgcgtcatt tgactcgttc gaccatcact atagaaacag aagcagagaa tagtcactta
    10261 gcacaattga acggtgtcca tgactttgtg caaaaaggtc acctcgctca atttgctgct
    10321 gataacgaag ctttcgatgt gattttagct gaagcgtcca aattaggtgt gaaaaaattt
    10381 gaatctattc caccgactct tgaggattta ttcatgagac attatgaagg atgaggagga
    10441 agcgaaatga ctgataaatt ttcacgctgg tccacccttt tttctcaaaa ccttagccgt
    10501 gactggaaaa agatcctgat atggctagtc ggtgtcactg ctttttctgg tggttttgtc
    10561 cctgcgtttg aagaattgac ttccggacaa ggattagctg gattatacga aaccttgcaa
    10621 aacccggcaa tgattgcaat ggtcggacca actccagctt cgaatgctgc tgagtatact
    10681 ttaggagcga tgtatgcaaa tgaaatgttg cttttttgca gtttgtttgc tgctgtgatc
    10741 tctgttttac acgtggtgag ccaaacgaga aaaaaagaag aacttggtct gattgaactt
    10801 attcgttcct ttcgagtcgg acgtcaagca aactctttag ctgtcatcgc tgaaagtatt
    10861 ctgatcaatt tgcttttagc aatatcgatc agctgtacga tgattttttt ccaagcggat
    10921 tcgatcactg cagaaggtgc ttggcttttt gggctatctg taggtagtat ggggattttg
    10981 gggagtgccg ttgcactgtt tgctgcacaa ctgatgccaa gttcagccag ttcgaatggt
    11041 ctgtctttag cgtttttagg tggactttac cttctacgag caataacaga tgtagctgat
    11101 tcctcccttt cgatgtggaa tcctttaggc tggtcttatt tgacctatcc atttacaact
    11161 aacaactgga ttcctttgct ttatttgttt ttgttcagtc taatcttgat aatcagttca
    11221 tttgttttag aggaacatcg agatttgaac accggctata tccccgaacg tgaaggaaaa
    11281 ccgtatgcta aacgttctct tttatccttg cgtggtttat tattctatct aaataaaggg
    11341 atgacaatca gttggcttat cacttttggt ttgatggggg cggcatacgg ctctatctac
    11401 gggaatatgc aacagttttt agaaagtaac gacttaatga aacagatgtt tatccaatct
    11461 ggcgtctcaa ttgaaaaaaa ctttacaagc acgatcatgg tcgtcttgat ttgtctagct
    11521 gctattttac caattgctag catcaatcgt ttatttacag aagaaaccca cggacgtatg
    11581 aatcagctgt tcgcaacaaa aatttctagg aaccaacttt attggaccaa tatgttacta
    11641 gctattctat ttgctatatt ggcaatcctg attagttctg gtgcactagg gctttctgct
    11701 ttggcttcga tgaaggatgc gaaagacatg gatctattga tgtttcttaa agccgggttc
    11761 aatttcctgc ctgccgtttt agtgtttatc ggaattagtg gctttctatt cggatggctt
    11821 cctcgaatcg gtaaacttgc ttatatctac ttagcttact cctttgcact caattacttt
    11881 ggcggaattt tggatttacc agaatggttt tccaaaacag ctgtccctag ctgggttcca
    11941 cggatgccaa acgattcttt tgatctttcc gtgttcctta cgttatcatg tatcagcctt
    12001 gcactgttcg tccttggttc tattggttat aggcgaagag acttactaga aggataataa
    12061 aaaagcatgt acaatttcat gctagcctcc aaatctgggt caaaaatcca acatttggag
    12121 gtcatttttt tatttacaag tcttctaact gctctttatc aatgatccag acttctcgat
    12181 tctttctttt tacataacca ttctcctcta ataatttaaa ttttctagat aaagtttcgg
    12241 gggtcgtacc gataaatgca gcaagttctt tcattttcat tggcaggaaa aagtgatcct
    12301 tccctgtcgc tttagctaag tcaagcagat agacagcgac tcgttcttct actttttcca
    12361 tcatcaaaaa agttgtctgc tgctgtactt ttgttaattt ctcaacgttg attgctaaaa
    12421 gtttcagact aagggcggga taatgcagta agagttccga gaaatctttt tgtctcaata
    12481 tgcaaatttc cgttttttct agtgcttcgc caaataaaga ctcatttgga acgcctagca
    12541 actggctctc tccctcatat ccgcccgact ctattactcg taaaagctgt tcctttccgc
    12601 tagcagataa ttgatacact ttcatactgc cggaggccac gatcactaat tgaggttctc
    12661 cattcggcgc aagaatctgt tctccttttt ccacatagtg atgtttgaca agtgcgtgta
    12721 ctttttcttg atcttgctgt actaagtgat tgaacaaagg aacaaatgaa acacataaat
    12781 gatcatgact cataatctct cctccttcta aacagctatt tctaataaag aaacataact
    12841 tcccttattc caactgataa ttttctttta tcatagcata aacgtcaagc gaaccagata
    12901 ccagcaaata gagtggattt ctttttgtcc cttgctttat tttccttctt ttgagacctt
    12961 aagtaattga ttcctatcaa ttttttaaat atttactttt tgaataatca aaaacaaacc
    13021 ttcactcgca agccgagagt aaggtttgtt tttcgcattg cttatttcgt gttgtctatt
    13081 gaaatttttc cgcacctagc ttttgatata gtggtgctag ttcttgtaag gtttcagcgg
    13141 ttatttgctc aacgtgttcg atttcttgcg gatagaagaa tagtttaccc atttgaaagg
    13201 ttcggtctct catcgctttt ttcaactctt cttgtttctt tgtatcggac aaatagtcag
    13261 acaaagctaa gtggtcgtca agttcatgtt ctggttgcgg ccatatgtaa taatccttga
    13321 tatcgactgt tttgctccac tctttcagta tcgcaagcaa ctggttatac gctgcaatgc
    13381 taccatatcg ttcttcgctt ttataatgtt ggtacatcaa atagatttgg tattgttttt
    13441 gattcaacaa aactgctaga caggcattct cgtcttgacg agattcgctt cggtacgcgc
    13501 tccaaaaatg actgcgtaaa ttccagccat tcgtccagct ttcgatttta ggtttgctca
    13561 tgccgtacat atcaggcaaa agtgcagctg tctggagctg aatctctttc catttttgcc
    13621 agtgttcttt atattctttc ttgatttgtt caatcttatc tgttggatgg ctctctttga
    13681 gctgtttgaa tgcgtagatt ggtcgatcaa acaactgata atctgatttt ccaagttcca
    13741 ttgtttcgcc tctttccgtt atcttttgta ttctacctga attttgcgat atggacaaat
    13801 agaggttgtg aaaaagctgt tctgcatcaa gtcataagaa cagccaaaca aaaaactgcg
    13861 atgagcatca ccgcagtttt attggctcta taat
//
LOCUS       DACECY010000069        13733 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.073, whole genome shotgun sequence.
ACCESSION   DACECY010000069 DACECY010000000
VERSION     DACECY010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.073"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(63..1268)
                     /locus_tag="IWO38_002240"
     CDS             complement(63..1268)
                     /locus_tag="IWO38_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0314313.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(1291..1444)
                     /locus_tag="IWO38_002241"
                     /pseudo
     CDS             complement(1291..1444)
                     /locus_tag="IWO38_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(1447..2832)
                     /locus_tag="IWO38_002242"
     CDS             complement(1447..2832)
                     /locus_tag="IWO38_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ0314314.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(2861..3247)
                     /locus_tag="IWO38_002243"
     CDS             complement(2861..3247)
                     /locus_tag="IWO38_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0314315.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(3263..3577)
                     /locus_tag="IWO38_002244"
     CDS             complement(3263..3577)
                     /locus_tag="IWO38_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0314316.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="IWO38_002245"
     CDS             complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="IWO38_002245"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ0314317.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            4837..6126
                     /locus_tag="IWO38_002246"
     CDS             4837..6126
                     /locus_tag="IWO38_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ0314318.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(6165..6770)
                     /locus_tag="IWO38_002247"
     CDS             complement(6165..6770)
                     /locus_tag="IWO38_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ0314319.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(6966..7430)
                     /locus_tag="IWO38_002248"
     CDS             complement(6966..7430)
                     /locus_tag="IWO38_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0314320.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(7452..7772)
                     /locus_tag="IWO38_002249"
     CDS             complement(7452..7772)
                     /locus_tag="IWO38_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0314321.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(7915..8994)
                     /gene="pepA"
                     /locus_tag="IWO38_002250"
     CDS             complement(7915..8994)
                     /gene="pepA"
                     /locus_tag="IWO38_002250"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ0314322.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            9194..9544
                     /locus_tag="IWO38_002251"
     CDS             9194..9544
                     /locus_tag="IWO38_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0314323.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            9729..10682
                     /locus_tag="IWO38_002252"
     CDS             9729..10682
                     /locus_tag="IWO38_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0314324.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            10695..12482
                     /locus_tag="IWO38_002253"
     CDS             10695..12482
                     /locus_tag="IWO38_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314325.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            12469..13671
                     /locus_tag="IWO38_002254"
     CDS             12469..13671
                     /locus_tag="IWO38_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314326.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT         3938 a         2689 c         2520 g         4586 t
ORIGIN      
        1 aggttttgtc caaaattcat aaaaatgttc tcctctctat attcaatgaa tttgtatttg
       61 agttattttt ttgttgttat cacgtcctgt tcttttactg actgttgctt caaaatctgc
      121 ttgtgtcggt ctgtcagttt cgcatggtcg agaatgtctt ttacaacctg cgtctggttg
      181 atttcatcaa gtttaatcgc aacctttaag gtcggggcaa cttgatgaga tagccagttc
      241 agcgtccttt ggaaggagta aggctctggt tttgtggtta gttttaatcg ttcacgattg
      301 ttcccaataa accaagccca ttcttcattc agtttccaat cagaacgagg tttggaatcg
      361 tctttatcta caaaacggat ataccgattg ataattttaa aggcggtatg ctctggattg
      421 tcatagacga gtaaatcacg gactgcataa taggcacgct catttttcaa tcgaatctca
      481 aaacggtttt ttacttctgc gtcttcaatg ggaatatcat ttttcttgta ctgctcgtag
      541 tccttttcat agatacagaa ataaacttca ctttgtaatg aaccgatata gagggtgttt
      601 cccatacatt ccttttcctc tttgcgtacc agttcgccac tgcgatagct tttaaaactg
      661 cggaagacgg agatacattc ttcctgttgg cacttttcag tgagtacagg gatatttaaa
      721 atccctgtct tatcgttaat ggcaaggtca aggcgtttca tcacaccgcc agccaccaaa
      781 acgtccataa agaactcata ccagcttctt tgttgtgcca gaagatagct ttcaaattgt
      841 ctgcacccac gacctttcaa ttccaccaga actcctttgt ccagttcatg ggagcaaagg
      901 acgaatatgt cgcctaaagc ataatgctct gaataagaat agaaaccata gtcctcatga
      961 agaaaatagg acagtttcag ttgtaagatg ttttcgacca cctgctgtac gtctgttgtc
     1021 ggaaagcgaa ttcttacata atcaaacagc atttcaaggg gagcgtcggg attgaagcgt
     1081 tccagagctt cccaaaggga ctgctgtaaa tcctctgatg gcttgacttt tcctgtttca
     1141 atatcgctta gatactgcct tgtaatacca gtcgcaacag ctaaacggtt ttgagatagt
     1201 ccataagcca agcgtttttc ttttaaatgc tgtaaccaag tttgttcatt cagtaaaaat
     1261 ccctccaatc aaaaaggcgt atgtcaactt ttaaagccca tttgacatac gctgaaattt
     1321 tgtaaatccc ttgtaaccaa aggattttct aatgtttttt tgactgtttc ctgtcgattt
     1381 gtacccccct gttagatacc gaggggttaa gtgctggcgt ggctattgcc acaccagcca
     1441 gcaagatcag tccacacctg cgacttccgc ttcgcacgtc gcctgcgtgg actgtctgct
     1501 gttggataac tttttaattt cctccaagaa atcatatcct tttggtacaa ggggagtata
     1561 aaactctgat atgacacttg ttcctacatc aacatagcca cgacctttga ttcgctttaa
     1621 gaagaaatcc ttttgtacgt cactgccaaa catcatgcca tagcccattt cagacatacg
     1681 acctaaagcc actctgaaat taaactgatc acggattccg tcgcctaaat attttgcgtc
     1741 tggacgttga caagccagta ttagaaagaa gccagcttga cgacctaaca tgacaatctg
     1801 tttcagctta ttcataactg cggtgttttc ttttgttccc agcatttcca tgaaagcgac
     1861 gtattcatca aagattaaga agtgtgccgg gagacctaag taagcataat ttttgccagt
     1921 cttatagttc ttcatctgct tcatttcctc actacgtttc atcatttctt catagaatgt
     1981 ttcaatgcaa gaaagcaagt cttcttttct atagtagaca tttgccatca cagaacctaa
     2041 gtccgcaagg tcagcatttt tcgggtcaag aatatacagt tttgaatctg tatgaagcaa
     2101 ggcttcaatc agtgtcagta taaagtaagt tttaccgcca cctgtaccac cagcaatcaa
     2161 catatgaggg agcttatcat attcccacca tacgtttttc attaagcgaa gtttaccatc
     2221 tttagcttct acttcatcaa tagaaatacg actggctatg gtgtcataga gcaaagtata
     2281 ttccacatag gaatccttta actctttatc cgtcagctca cagtacaagc cactctctaa
     2341 tttcttttcc aagtgtaaga gttggtcttg atattttccc agcgtgattt ccacccgtat
     2401 ctgtatcaag ccatttttaa gtcgataata cattttaggg aagtaggtta tcttttcctt
     2461 tgtacgacca gcactatctt taaagaaacc ctctgttttg acctgttcag attcatacca
     2521 cttgttttca agtatcatct ttgccagttt ttgacggtgg taaagttgtt taaccgtatc
     2581 atagcgaacc cgtttgaata caaacgctac cagcaagcag ataagaattg cgacactgaa
     2641 actgataatt aaatagggaa tgtcaatctt atctgcttgt gataggttaa aatcctgcca
     2701 gttgatctgc tggattgtct tcacatgaaa cagtccgaca accagcagga aaacaggcag
     2761 gagtgacgct atcgtaaaat gaaagactaa atctttacca gatgggcgaa tccttttacc
     2821 acgctgtttc atgcgaaaaa gtctcctttc tacctagcga ctatttgtct tgtgtcggtt
     2881 ctttctttgc ttgtggttga gctttgaatg aactagaatc ctttgtcagc acaatatcgt
     2941 ctgccttgat ataccagtca acatctgctc cttgataggt ggcagtagca acggtgtccg
     3001 caatgggatt gataagttcc acccgtgcgt tataatcaaa ctctttcaaa ggcacgctgg
     3061 caggaatact tacttgaatc atgcgtcctt gtcctttgga ttttaagtca taggtacgtt
     3121 ccttgatttc atctgaaacc gacccgtctt cattttggat tctcacttca cgacgtagag
     3181 cagagaattt caattctcca aaagtcgtgt ctttatctaa tacaatgcca tttgctaatc
     3241 tcatcatttt tcctctcttt ctttattctt ttatcatgtc gtcagcatgt aaaaggtaat
     3301 ttgtaaaacc acgagtgccg attttgtagc cctctgcggt aatacgtgga ttgactaact
     3361 tcacacgttc ctcaaagccg aaatgttttt cgccagcttc agcaggaagc accaccacaa
     3421 tatcatctgc tctttgaaca tcagaataga gattatagct tcttgataag acagttagcc
     3481 gtccgttgat tcttcgctga acgactttat cctcgccagc aaattctaaa ttgccgaatg
     3541 ttttttccat gttgggaatc acaaatttaa gttccatatt tttacctatc ctttcttttt
     3601 tattggctga atgaatgttt gatggtctta aagagtgggg aacgaccttt tgattcttga
     3661 ttttttgttt tcataagttc acttcctttc aaaatcgggt aaaaaaatag acacctcatt
     3721 ttttgaagtg tctacctatt aaatattcaa attttattgg aagtatcttt atatcttcac
     3781 ttttcaagga taaatcgtcg tatcaaagct cattcataag tagtaaatta gtagtaaatt
     3841 gagtggtttt gaccttgata aagtgtgata agtccagttt ttatgcggat aactagattt
     3901 ttatgctatt tttctcttaa aaaatatcat tatttgtggt atggagcccc cgcgttgatc
     3961 cggaaggcac ggtagatttg ctcgacgagg atcaggcgca tcaattgatg tggatacgtc
     4021 attttgccaa aagagatctg agcattgctt cttttcatga cggcttcgct gagtcctaaa
     4081 gaaccgccga taacaaaaac aaggtggctt tttccttgga tacctaattg gtcgatttgt
     4141 ttagcaaaag attcgcttgt gggatttttc ccttcaattg ctaagccata gacgtattct
     4201 tgttctttga ttttcgctaa gatccgttct ccttcttttt cttttacttg tctcatttgc
     4261 gcttcgctta aattctccgg ggctttttcg tcaggaactt cgatcaattc gattttcgca
     4321 taggcattca gccgtttgac atactcattg ataccttgga tcaagtattt ttcttttaat
     4381 tttcctactg aaataatttt gatattcatg ttttctctcc tttgatcttc acgctcactt
     4441 tgtttattat aatcaatttt ttcgtctata tgtttatttt gactattttt gttaaaaaga
     4501 tttccccaac agttatgcac aacatccaca ggtttatcca cagaaaacag cgttgtttta
     4561 ccttatttca tcacattcat taggcaaaca tacgttttat aagaatttat ccactttata
     4621 cacaggctgt ggatatgttt tatcccttat ttttccaatc ctatccgcgt agttttacac
     4681 aatatctgtg gaaaaagaaa atcaaatatt ttcatctgaa aacaatgatt tttcctaaag
     4741 tttttcataa aaagttcaaa gtctcaaagt agactaaagt caacaaataa gactatacta
     4801 acagtagata aatgaatgta attggaggag gaacacatgg atagaaaaaa tgtgacacca
     4861 aagatgaaaa aaaataaaaa taatagcctc tggcgtaagt tgggtctggg gcttgttgga
     4921 gggatcgttg gaggactact gacagctggc attttttatg cagcgatggg cactggcaac
     4981 aatagttcta ctgctacaag cggcaatcaa aattctgccg gtgaaacagt tgtggaaaat
     5041 gtcaaagtca atgtagattc tgatatcacc aaagcggtag ataaagttca agacgctgtc
     5101 gtttctgtta tcaacttaca aagtcaaaac caatcaagcg gttttggcgg attattcggg
     5161 cagcaagaag aaagtagcag ctcagatgat agtaatttgg aagcttatag tgaaggaagc
     5221 ggcgtaatct acaaaaaaga cgggaatact gcttatgtag tcacaaacaa tcacgtagta
     5281 gatggacagc aaggtttgga agtcatgatg aaagacggta cgaaggtaaa agctgaatta
     5341 gttggaacag atgcctattc agatttagcg gtgttgaaga tcaattcaga caaagtcgaa
     5401 acggttgcct cttttgggga ctcaagtgct ttgaaggttg gtgaacctgc gattgcgatt
     5461 ggttccccat taggatctga atatgcgaat tctgtcactt caggaatcat ttcttccttg
     5521 aaccgacaag taacaagtac gaatgaatcg aacgaaacag tcaatatcaa tgcgatccaa
     5581 acagatgcag cgatcaaccc gggaaattct ggtggtcctt tagtgaatat cgaaggacaa
     5641 gttatcggta tcaactcaag taagatcgca agtacttctg aatcgacttc taatgtaagc
     5701 gtagaaggaa tgggatttgc catcccaagt aacgatgtag tgaatatcat caaccaactg
     5761 gaaaaagacg gaaaagtgac acgtccagct ctaggtatca caatggtcga cttatctgcc
     5821 gtttctactc agcaacaaga acaaatcttg aagattcctt catctgtaac taacggtgtc
     5881 atcgtgacaa atgtccaaac agctacaccg gcagaaaaag ctgggttgaa acaatacgac
     5941 gtcattacta agatcgatga caaagaagtt tcttctggtg tagaacttca atctgctttg
     6001 tataagaaaa aagtgggaga ttctgtcaaa atcacttact atgaaggctc gaaagaaaaa
     6061 acaactactg ttgaattgac gatcgatcaa tcggcattaa aacaaaatca aaatagtgaa
     6121 aattaataaa aaagaggttg tgacaaagac ttgtcacaac ctctttattt tggaaatact
     6181 tctccgacta cggcatcctc aggcaatact aagattcctt ttttttctgg agcatttggc
     6241 aaatgcaatt cacgagcaga acaaatcatt ccatagctgg ctacccctcg caaagttcct
     6301 ggccagatca tcattccatc tggcatcatg gctcctggtt ttgcaacgac aactttttgt
     6361 ccagcttcga tatttggtgc accgcaaaca atttgcaata ctgtcccatt gtccacttct
     6421 gtctcagtaa tcgataaatg gtcagagtca ggatgcggga cgcatgtttt tacatacccg
     6481 acaacgatct tcggttcatg atcagcttca agctgttctt cgaatcctgc ttctttaatc
     6541 aatcgattca atttcgctaa ttcttcatct gttaagctta cttggccgtt cccttcaatt
     6601 gttagatgat cggagatatg gaaaaagttc caagcaacaa cttgaccttg ttcatctttg
     6661 atccgagcaa cattccattt acgttcgaca gtgttttctg ttcctttatc gtcggctatg
     6721 ataaccatca aagtgtctcc gacgtgtgct ttgttataag caaaaatcat gttctttcct
     6781 ccattatcct ttgctgagat ttatcttaac aaaaaattgc attcactgca tgagattttt
     6841 ttcatgcttt atttccgaag aagcttcttc tggaacacca tttattcgta acaagggact
     6901 gtgacaagac ttttgtcaca gtccctttac tgtttgatta aaaaggttat acaagttcca
     6961 cttgcttaat tactcggctg ttcttcttcg tcagaaacga tcaagacatc acaaggtgaa
     7021 tgacgtatga cgtagctggc tacacttcct gtcataaagc gttcgactgc atttagtcca
     7081 gactgcccaa ccatgattag atcgattgca tatttttttg gcaatttgac tgccatggat
     7141 tcacgaacag agccgtatgc aataattcct tctatatttg tgtaatcaac agattttcca
     7201 tattctttac attcttcgat catttgcttg gctgtttcag tttgctgatc gactagattt
     7261 tgatcaatag gagcatagcc cataaaatta taaaattgct gatcgattac gtttgctaca
     7321 tatactttcc cattattacg gcgagccact tctaacgctt ttttgaaagc agcaagtgct
     7381 tgttcactac cgtcaatacc tactaaaatg ttcttatatt cttgattcat cttgacccct
     7441 cctaacagat gtcagccttt tattacactt tctaggaacg attcgatttc ctgttttgtt
     7501 ttacgatctt tattcactag acgacctaat tcttttccgt cttggatcac gacaaaactt
     7561 gggataccaa aaatgttcca ttcagcggct acatctatgt acttgtcacg gtctacttca
     7621 ataaattgcc aatcagaaaa gtcattttcg atttcaggca tttgcggttt gataaaacgg
     7681 cagtctccgc accagccggc agtaaagaaa aatacacttt ttccagtttc tacgtagcct
     7741 gctaattctt ctaaactatt tgggataatc attctttttc ctctactttc tgtttatgta
     7801 tttttcaatt aaattataca cctttctttc gaactaacaa acattgatcc tctttttata
     7861 aaaaaaatct gacaaaaggt tgcttttgcc agattttata gtttcactat cgcgttattt
     7921 cccgtatacg atcgtgttca ctgttgattt atctaaacct ttcaatgttt gaatcaacat
     7981 ttctcgtgca gcttcaaaat cgcgaatact gaacatcgtt tgatgcgtat ggatataacg
     8041 tcccactacg ccaatcactg tacttgggat tccttcattt tgtgtatgag cagcacctgc
     8101 atctgtaccg ccttttgaaa caaagtattg ataagggatg ttgtttgttt cagcgatatc
     8161 tagcaagtac tcacgcaaac gaggaagcat gatcaaacct ggatcttgga tacgcatcaa
     8221 tgtaccttct cctaaatgtc cgaatgtgcc atttttcgtc actgtgtcat ctgctgcaga
     8281 gcagtctact gcaaagaaca gatctggctt gaatttattg acagaagctt tagaaccacg
     8341 caagcctact tcttcttgga cattcgcgcc tgcaatcaat gtatgaccta atgtttcatt
     8401 ttgtaacgct tctaaagctt ctaataccat tgtacagcca tagcggttgt cccacgattt
     8461 gctgatgata ttcttcccgt tggctgtttt gatcgtttca gcgtaaggaa cgattgtgtc
     8521 tcctggaaga acaccgtaac tcattgcctc ttctcttgat tcaaacccag catcgaacaa
     8581 aatatctgtt acttctactg atttttgtcc gcttgttcct cgtaataaat gtgggggaac
     8641 ggaagaagaa atacatggat aattcttacc agtagaggtt ttaagtgtaa aacgttgagc
     8701 agaaacaacg taagggttcc atccgcctag aggtacgact ttaaaaagcc cgttttcttg
     8761 gatttgtgtc agcatgaatc cgacttcatc catatgtgct gcgaccatta cacgaggtgc
     8821 atccgcttct tcatggtggc gcagcccaaa aatgccgccc aaaccatcta actgtaactc
     8881 atctaccagc ggttccatat gactttccat ataggcgcga acgtcttgtt caaaaccgct
     8941 tgttccttgt aattctgtta actctttgat tcgttgaaat gttttttctt ccatatgcta
     9001 ttcgtctaaa tttcagacta aactcctctc agacttatat tgatctgcgg ataaaatatg
     9061 attttttctg cagaccgatc gtatttatta tagcttattt tttctggtag cggttattta
     9121 tttcaaagaa aatatgagag tgtggtaaaa taaacacaaa gagtagcaag tgagaagaag
     9181 gaggtatatc cacatgtatg aagaaagcga attaagctat tttaaaggcg ggttagccgt
     9241 tgggatcggt ctaggattat tgagtggggt agcttcaacg atttggtatc atcgcaaaaa
     9301 agtgttatca gcagaccaag tgcttgaaaa tatcaaaaca tcttttttaa aagaaggcgc
     9361 aatcgaaggt gcttggatct cttttgaaaa agaacctatt cggaaattcg ccgttcggtc
     9421 gagtgcttat cggggtgggc tttcccggat agaagatggc gaaactgttt attatgaatt
     9481 tttagcagat gctcatacag gaaccgtatt agacatttca agaaaaaagg gcgcaataga
     9541 ttaatacgtc aaaaaaatga aataacgaat acgcacccac tatctaaaag agaggtatcc
     9601 tatccttctt ttatagtggg tgatttttat aaaaataggg ctatttcctc ttggaatagc
     9661 tagtatttgt agttattata cggtattctt gaaataagat tagttttatt atataaggag
     9721 agtgacaaat ggaatcacgt gaaaaaattg ccgtgctcat tccttgttat aacgaagagg
     9781 cgacaatttc aactgttatt gaagatttta aaagggaatt accagaagca gatatttatg
     9841 tctatgataa taattcgacc gatcgaacat acgaattggc cgttcaaggc ggagcaatcg
     9901 taaagaaaga accacgtcaa ggaaaaggga atgtaatccg tcaaatgttt tttgatattg
     9961 atgccgatta ttatttgatg gtagatggtg atgatactta tcctgcagaa gctgcacatg
    10021 gtttgcttga gaaattacgt tctggcgaag cagatatggt cattggcgat cgtctatcaa
    10081 atggaaccta ttttgatgaa aataagcgtc ctttccatga ttttgggaat aatctggtta
    10141 aaaatacaat tacttactta tataaaacaa aaattcgcga tgtcatgacc ggctatcgag
    10201 gattcaatcg aatttttgtc aaaagcttcc ctattatgag ttctggtttt caaatcgaaa
    10261 cagaattgac gattcatgct ttagataaaa aattcaagtt cgttgagatt ccaatcgatt
    10321 atcgcgatcg tcctgaaggt agcgaatcaa aattgaatac attctctgac ggattcaaag
    10381 tgatcatgat gatcatcaaa atgtgcaaag attataaacc tttgcttttc tttgggatct
    10441 ggaccatctt cttcttttta tttggattgg tcatggggat tcctgtgatt cgtgaatata
    10501 tgctgacaag ctttattaca cgcatccctt ctgcgatttt aagtactgga ttgatgactt
    10561 tagcccttct ttcattagta acagggttga ttttggatac ggtcgtcaca aatgccaaga
    10621 aagaatatga attgaattta taccatgtct accacgaata tcaaaataaa gaaatgaagt
    10681 aggaggcctt ttttatgcat aagaaaaagg agaatctatt tcctactttt tttgcaactt
    10741 tatttgcgtc aggttttgct ttttttactt cttatggtct aggaactttg aatccaacag
    10801 taatcattgg tttagccatg gttttgctac ttgcttttaa gcaatatgga atagagttgt
    10861 tttcaataaa aaacaaagtt gcagctattg cccgattgtt tttttctgca gcttttgctc
    10921 ttacactttc tattggcaga cagatacaat ttcagtatgt ctcagcaaac tatacaaaga
    10981 caacgattgc tgatggaagt attttttact ttcttgttta tttctccatt tttgtgtatt
    11041 cttttttatt tttacaatcg atttatttat caatgccaaa actggaaaca ttttttactg
    11101 ctcaaaacac acaggctttc tcttcaagaa aatatttttt attctttact gtctttttag
    11161 ttctgtgttg gcttccttat ctgttggcat tgtatcctgg agtggttttg cctgattcgt
    11221 taagttcggt cgcccaatct atgggtgatg ttcctataag caatcatcat ccagtcttat
    11281 tcaccttact ggtcaagcta tttgttcatg atttaccaac tgatacgatc aatcacggtg
    11341 tatttttatt ttcgattttt caaacattga taacagcttc agcgataagt tattctcttt
    11401 gctggctgtt aaaaagaaac atccattttt ttccggtact cattagttta tgctattttg
    11461 catttgcacc ggtttttcca atctacgcct tgaacatgca aaaagatact ttgttctcta
    11521 tcgcttgctt tttattttcc ttagcggttt ttgactttat ttttcaacga gaaaagccaa
    11581 cgattctcaa tcagatttac gtaatcatcc ttgctttact gaccacctat ctaagaaata
    11641 atgggattta tgtagtaacc ggcacaggtt tgatttgcgg cttgattatt ttgaaacaaa
    11701 aaaggtatca agcattcttt caagtggtgc tgggttatac agtgattgct ggactactca
    11761 tccacccgtt gatggctcgt tatgctattc ctacagaaac tgctgaacaa ttaggtgttc
    11821 ctttgcagca aatttctaga actattgtta tggatggtga cttgtctaag aaagataaag
    11881 cattcatgaa tcagctactc ccgataaaag attatgaagc gtattctcct agtcttgcag
    11941 actcaatcaa gtggcataaa aattttaaca atgagttttt gaatacacat aaaaaagaat
    12001 ttctatcact atgggcaaga aatttaccaa gtaatataaa aatctataca gatgcttata
    12061 ttttagaaac gttcgggttc tgggtacctg gtacaaaaaa tagttacggc tttttagata
    12121 ctcgtgtaaa cacgaataat tatggtatga agcaagtaga tttgattgaa catcttactg
    12181 gattgtcagg aatgaaaaaa atcatcgatg ctcgtgattt ctttggttcc ggcacattac
    12241 tgtggttagc tatcggcagt gcacttttag caatctttaa gaaaaaatat cggtatttac
    12301 tcctttttct tcccggtatg cttgtttttg ctactattat gattgctact cctgtggctt
    12361 ttagtttacg gtatgtgttt gttttagcat taggtctacc tgtttatatc ctctctcctt
    12421 ttttactaga taaaagagaa aaaatgaaaa cagatatagg agatactcat gaaactattt
    12481 aaaaagatat tttttgcttt tgttctcttg atcgctagct attgcgtact actgacaatt
    12541 acttttttca ttccgcagca ctctattgaa tcgaatgcag aaaaatctct acagatggtt
    12601 gaaaaagaag gaatgtatcc ttctatcaat caaggaaata tgctaggcac tcggttagat
    12661 aattttactg atcatttgat gatacgaaaa acaaaagcag atcctgaatt gaactccttg
    12721 gagaacgcga tgtctatggc ggattatccc cgttattggc atggttatca gcttttttta
    12781 cgacctttac tgctagggat gagtttgggg agtattcgaa tgatttacgc agccattctt
    12841 tttctgctga ttggattgac gagctacttt ttaaccaagt gtagcgacat ctatttaacg
    12901 acagccttgt tgatttcgct tgctatagga aatgctgcaa cctttttctt cagtatgcaa
    12961 ttttcaaata tttggatttt gactttattg tctgtattac tatttttaac aaaaccacaa
    13021 ctctccaaca ccaaccaaca attattcttg tatttctttg tcattggttc tttagctaac
    13081 ttttttgact tgttgacaac accggtcatc tcttggggga tacctgtgat cattatttat
    13141 tatgtaacaa ataagtattt aatggataaa cgcagttcat tgagcaaaca agtaggcagt
    13201 tttgttttta ccggtatctt ttggggatta ggctatggtt tgacatgggt ggctaaatgg
    13261 atcctatcat cgatcatatt gaacaaaaac attgtaaaag atgcaatcaa ccaaatcctc
    13321 tttcgaacgg aaggaaacga caaatacccg ttgcatcgat tagagatgct gaaaagcaat
    13381 attagattga tgtatccaaa agtagcaata ttattgctat tgattacttg tcttgttttt
    13441 ctctattttg cttatcgtaa acgaaatagc tggcgtccgt tccaacttct agttttgttt
    13501 gttttagctg ctgtaacgcc gtatatttgg tacaatattt tagctaatca ctcacagatt
    13561 cattattggt ttacgtatcg tacgcaaatc atcacaagtt ttgctatact tagtgctttc
    13621 gcctttttga tccctcctga gaaatcaaaa gacgaactaa attactttta agaataccga
    13681 tgtactaatt catttttaaa cataaaaact ccagctgtca gagatagccg gag
//
LOCUS       DACECY010000070        13226 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.074, whole genome shotgun sequence.
ACCESSION   DACECY010000070 DACECY010000000
VERSION     DACECY010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.074"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(69..626)
                     /gene="frr"
                     /locus_tag="IWO38_002255"
     CDS             complement(69..626)
                     /gene="frr"
                     /locus_tag="IWO38_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ0314327.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(629..1351)
                     /locus_tag="IWO38_002256"
     CDS             complement(629..1351)
                     /locus_tag="IWO38_002256"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ0314328.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1487..2368)
                     /locus_tag="IWO38_002257"
     CDS             complement(1487..2368)
                     /locus_tag="IWO38_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ0314329.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2467..3249)
                     /gene="rpsB"
                     /locus_tag="IWO38_002258"
     CDS             complement(2467..3249)
                     /gene="rpsB"
                     /locus_tag="IWO38_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ0314330.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3608..4087
                     /locus_tag="IWO38_002259"
     CDS             3608..4087
                     /locus_tag="IWO38_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ0314331.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4304..5395
                     /gene="phnW"
                     /locus_tag="IWO38_002260"
     CDS             4304..5395
                     /gene="phnW"
                     /locus_tag="IWO38_002260"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ0314332.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5388..5516
                     /locus_tag="IWO38_002261"
     CDS             5388..5516
                     /locus_tag="IWO38_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ0314333.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5520..6065
                     /locus_tag="IWO38_002262"
     CDS             5520..6065
                     /locus_tag="IWO38_002262"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ0314334.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6519..8210)
                     /locus_tag="IWO38_002263"
     CDS             complement(6519..8210)
                     /locus_tag="IWO38_002263"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ0314335.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8630..9577)
                     /gene="arcC"
                     /locus_tag="IWO38_002264"
     CDS             complement(8630..9577)
                     /gene="arcC"
                     /locus_tag="IWO38_002264"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ0314336.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9692..10711)
                     /gene="argF"
                     /locus_tag="IWO38_002265"
     CDS             complement(9692..10711)
                     /gene="argF"
                     /locus_tag="IWO38_002265"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ0314337.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10802..12031)
                     /gene="arcA"
                     /locus_tag="IWO38_002266"
     CDS             complement(10802..12031)
                     /gene="arcA"
                     /locus_tag="IWO38_002266"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ0314338.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12492..13193
                     /locus_tag="IWO38_002267"
     CDS             12492..13193
                     /locus_tag="IWO38_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0314339.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         3718 a         2697 c         2361 g         4450 t
ORIGIN      
        1 taaataaaag aaatataaat aaaaacagag gaagcggaat gatccgcccc tctgttttta
       61 tgattttatt aaacttccaa taattctttt tctttatcag cagcgatctt atctacttct
      121 ttgatgctgt tatctgtcaa tgtttgtacg tctttttcta atccgcgcaa atcatcttcg
      181 gtaatatcac catttttttg ttgtttttta tattcatcga tcgcatcacg acggatgtta
      241 cgaatcgcga tttttgcatt ttctgcttct tttttcacat ctttagctaa ttcttttctg
      301 cgttcttctg tcagttgcgg gataactaag cgaatgacat ttccatcatt ggttggactg
      361 attccgatat cacttgccat aatagctttt tcaacatctt gaagaatgct cttgtcaaaa
      421 ggtgtgatca ttaaaacacg tgcttctgga atttggattg atgccatttg gttcaatggt
      481 gttggcgcac cgtagtagtt cacagtaata cggtccaaaa ggctagcgtt cgcacggcct
      541 gcgcggattt gcccaagttc acgttgaaga ttttgtgctg cttttttcat tttttctttt
      601 gcttctgtca taattgcatc tgccattttt atttccccct tacagttgtt ccgatatttt
      661 cgcctaagat tgcacggcga atatttccgt gttcatttaa attgaagaca agcagtggaa
      721 tatcattatc catgcttagt gaacttgcag ttgaatccat gacttgtaat cctttggcaa
      781 taacttccag atgagtcagt tcttcaaatt ttactgcatt tgcatctact ttcggatcag
      841 cagaataaac accgtctaca ttgtttttag ccatcaggat aacatctgcg ccgatttctg
      901 ccgcgcgtaa tgctgctgtt gtgtctgtcg agaagtacgg gttgccggta ccgcctgcaa
      961 agataacgat ccgtcctttt tctaaatgac gttctgcttt acggcgaata tatggctctg
     1021 cgatttgacg catttcaatc gaagtttgca cgcgtgtcgg tacacccacg ttttccaacg
     1081 tatcctgtaa agctaaggca ttcataactg tagctaacat acccatgtaa tcagcttgag
     1141 cacgttccat gcccatttgt gctccgattt gtccgcgcca gatatttccg ccgcccacaa
     1201 cgattgccat ttcaacgccc agctcgtgaa cttctttgat ttcttggatg atttctttga
     1261 ttactggtgg tttgattcca aatcctgcgt ccccagctaa agcttctcca ctcaatttta
     1321 agactacacg ctgatatttc ggtgtgacca tgttcattcc tccaatattt tctattgcca
     1381 ttttaccata cttagatgat ttcgacactc taagactggt ttttcttgtt ttgcttaaaa
     1441 aaagggagcg cacaaaccgt gcgttcccgt ctgttattca acagaattat tttttcactt
     1501 ggctcataac ttcttctaca aagttatctt cgcgtttttc gataccttcg ccaacttcga
     1561 aacgtacgaa tgttttaaca gttgcgcctt ttgatgcaac gtatttttca acagtcatat
     1621 caggatcttt aacgaatggt tggtccacca aagcgatttc tgctttgaat ttcttcaagc
     1681 gtccttctac cattttttca acgatattcg ctggtttgcc ttcgttcaat gcttgttctg
     1741 taagaacagt tttttcatgt tctaattcag cttctggaat ttgtgattcg tttacataac
     1801 gaggattgat agctgctacg tgcatagcta cgtctcttgc aactgtttcg tcagttgttc
     1861 catctagaac agtcaatacg gcaatacgtc cgcccatatg caagtatcca ccaaatgcag
     1921 cattgtcgtc tttttctaca acttcaaagc gacggaagct gattttttct ccgataactt
     1981 gagttgcttc gatcaagtca gattcaattg tacctttatc tgttttgatt ttcattgcag
     2041 cttccatgtc tgctggtttg ttttcagcaa caagttctgc aatttctttt actaaatctt
     2101 ggaacatttc gtttttagaa acaaagtctg tttctgagtt tacttcaacg attgctgcag
     2161 tgttaccttt cacagcaact gaagccaatc cttcagctgc aatgcggtca tttttcttcg
     2221 cagcctttgc cattcctttt tcacgtaaaa gatcaactgc tttttccatg tcgccttcta
     2281 cttcaactaa tgcttttttc gcatccatca ttcctacgcc agtcatttcg cgtagttctt
     2341 ttaccatttt agctgtaacg tctgccatgt ttaactcctc ctgtgtgttt ttctttttaa
     2401 aaaaagctgc ttcaaaggag agtgaggctt gtcgcccagc ctttgaaaca gctccatcat
     2461 tcaaaattat tctgctgaag aattgtcgcc ttcaacaaca tctacgattt cttcgattga
     2521 tgttgctgtg ttttcttctg tgaaatcttc ttcaactact tgatcttcac cttggtttcc
     2581 ttcgatgaaa gcatcagcca tttttgaagt aatcaattta acggcacgaa ttgcatcgtc
     2641 gtttgaaggg attactacgt cgatttcatc tggatcgcag tttgtatcaa ccatagctac
     2701 gattgggata tttaatttgt gtgcttcttg aacagcaata cgttctttac gtgggtcaac
     2761 gatgtacatt acatctggga ttcttggcat atcagcgata ccgcctaaga atttttcaag
     2821 acgttcacgt tctttgttca aaccagcaac ttctttttta ggtagaactt caaaagttcc
     2881 atcttcttcc attgcattga tttgtttcaa acggctgata cgtttttgga tagtatccca
     2941 gtttgtcaat gttccgccta accaacgatg gttaacgaag tattgtcctg cacgagttgc
     3001 ttcttctttg attgcttctt gtgcttgttt ttttgtacct acgaataatg ctacgccgcc
     3061 ttcttcagca acgctcttca tgtaatcata agctgcatct actaatttaa ctgttttttg
     3121 caagtcaatg atgtagattc cgtttctttc tgtgaagata tatttcttca tttttggatt
     3181 ccagcgacga gtttggtgac caaagtgtac gccggcttct agtaattgtt tcatagaaat
     3241 tactgccatg tttgtttcct ccgatttggt tttatttttc ctcttcttat cttctacctt
     3301 gccggcaaac tttttcaagc acctaccgac aatccaataa gaatgtggat ttgctgatgc
     3361 ataaagcacc ctcagctatt atacagacaa aagatccgta tcgcaagagc aaatatactt
     3421 ttctgctcaa cattcaatag tttacctgaa agataaaaag aattcaagaa aaacagtaga
     3481 tttctctttg tttctttatt atcctttttc cataaaataa ttttaggtat aaaagacgaa
     3541 tattattggg caatccttct tttttttgtt atgatacatg tgaaatcttt cgtaaaggag
     3601 aaagaaaatg cgcaaagctc aaagacattt gctgattaaa caagtcattg aagaattccc
     3661 gatccgtacg caagaagaac tattaaacaa acttgggcat tatggcgtta ccgccacgca
     3721 ggcaacgatt tctcgagata tccgtgatct aaaaatcgtg aaggcacctg atgaaaatgg
     3781 cgtttcccgc ttcgtattgt ttcgcgaaaa aggaactcct gagactagga ctgaggaaga
     3841 ggatcggctg gttcaaatga ttgaagacat tgtattgaaa gtcgaacgag tccacttttt
     3901 gacgatcgtc aataccttgc cagacaatgc tcatttattt gcatctgttt tagatgagat
     3961 caaacctccc tctattgtca gtacaattgc tggattcgat acgatcatgg tcatctcgga
     4021 aaatgaggag gacgcaaaac gagtagaaga atttcttcac catccaactg aagtatcact
     4081 tttttaatca gagcacgtat tcctaatatg gaatgcgtgt ttttttattt tcaatatttt
     4141 ccttattgaa taaaccttta cactctttac aaaaatacat cattttttat tcaaattttt
     4201 caacaccttg attgaatcac aataatcatc atttatattg gatgttaaca agagcagctt
     4261 cttgctcttt gtgtatttca aaaaagaaat gaaaggaaat catatgtata aattattaac
     4321 ttctggacca ttgacgactt ctgaaacagt gaaaaatgct atgctgatcg atcattgtac
     4381 ttgggatcaa gaatacaaag aaattacaca atggataaga aaaaaattat tggatcttgc
     4441 tcaagtatcg gaaggaatat acacaacggt cttaatgcaa ggtagcagct ccttcggggt
     4501 agaatcggta ttaagttctg tgatcaggcc agaagatact gtactgattt gttcaaacgg
     4561 cgcatataga caacgaatga tcaacatgtg cgaatgtctg aaactgaact ataccgttta
     4621 tgaggtatcg gaacatcaaa tcctccaagc tcatgtgatt acacagctga ttgaagagaa
     4681 tccgtctatc tccgcactag caatggtcca tagcgagact acttccggta ttttgaataa
     4741 tttggaagag attgctgctg tcacacaaga aagagatctt ttgtttatag tggacgcgat
     4801 gtcaagcttt ggtgggatac cgatttgtgt agccgaacta gggattgatt ttttgatttc
     4861 cagctcaaac aaatgtattc aaggtgttcc aggcttttct tttgtacttg ctaataaagc
     4921 ggaactggaa aaacgaaaaa atgttagccg aagtttgtca cttgatctat atgaccagta
     4981 ccgcgtgatg gaaaacgaga atggcaaatg gcgtttcact tcccctaccc atacggtttt
     5041 ggcttttcaa caagcgctga aggaactaga ggcagaagga ggaatcgaaa ctcgatattc
     5101 acgttatctc gccaataatc atgtgctacg acaaaaaatg acagagatgg ggttccagcc
     5161 tttcattcaa gaatatcagg ggccattcat cacctctttt ctttacccta aggactggcg
     5221 atttgatttt aatcattttt atcagtattt aaagcaaaat ggatttgtga tctatcccgg
     5281 gaaaatcagc gatcatgaat gttttcgtat cggaaatatt ggcgagatct atccggaaga
     5341 tattttggaa ctgacagcaa ttatcaaaga ctataaggag aacgttaatg attaaaactg
     5401 taatttttga ttgggcagga acaactgtgg actttggctg tatggctcct gtccacgctt
     5461 ttagaaatgc ttttctagaa aaaggaatcc aactgactga caaagaaatc cgctaaccga
     5521 tgggaaaatt aaaatgggat catatccaac agcttctttc cctcccttct gtcaaaaaac
     5581 aatggatagc tatttatgga caactacctc aggaaaaaga ggtggaggaa ttgtatcaac
     5641 aatttgaaca ttatctgttt aaagatttgg tcaagcattc taccttgaaa cccgacacat
     5701 tggaaacgat cgaacggttg agaaacgctg ggatcaatat tggttccacg aatggattta
     5761 atgctcctat gatgacaatc gtagcagaaa ccgcaaaaaa agaaaggtat tcaccagact
     5821 tcatggcaac ttttgaagac gtcgaagggt atggtcgacc gtttccttat atgatccata
     5881 aaaacatgca gtacttcaaa aacaagtcgg ttgatgaagt aatcaaagtc ggagatacgg
     5941 tctcagatat ccaggaaggg aaaaatgccg gagtgttgac agtaggtgta attgaaggta
     6001 gttcgctaat ggggatttct tatgatgagt accaaaaatt agagatgggg aaaaagatcg
     6061 tttaaaacag tctgttgctc ttagattcca acaagccggc gctgatattt gtgtaaacaa
     6121 tttaaaagag ttaacagaat acattttgta cgctgacaag aatccagaat gacttttata
     6181 caaaataaga ggttgtgaaa aagctgtcct gtatcaagta ataaaaacag ccaagcaaaa
     6241 atagctcctc atatttttcg tttggttagg cttattaccg cagatgcaag cttttgaaca
     6301 ccgtttattc gaaaaaaaaa agggagctgt gacacgactt ttgtcacagt ccccttattt
     6361 gcaaacaaac agtgtttaag agacaacctc tccactaaac ggtgttcatt cagctgactc
     6421 tcggtatttg ctcaaaaaac gataaaattt gaaaagctat tttctctttt gtttctctaa
     6481 tactcggatc agaacgctga tttcacattc tctttttctt acatcttatc tggtgcatga
     6541 agtcctagca aacgaaggtc ttctttcaat aatacagtca ctgcataaac tagagctaag
     6601 cgtgcttctt tttgttcatc atcagctaaa atcttcgtat gagcatagta tttgttgaat
     6661 gcttgtgcta atttgattgc atgtttcgca atcactgacg gttcgtattt ttccccagca
     6721 gacaagaccg tttcagggta tttttgaact aatttaacca cttcccagct ggtatcgtca
     6781 ttcaatgcat agtctgctgt ttctgaaggg acaaatcctg ctttttccaa caggctgact
     6841 gcacgagcat gtgtatattg cacataaggt ccagtttctc cttcaaatcg aacaacttct
     6901 tccaggttaa agtcaaatgt attcagtcga tcgtttttca aatcgtggaa aatgactgcc
     6961 ccaaccccaa cttgtttagc tacagcatct ttgttttcca aatcagggtt tttctcgctg
     7021 atttgttctt tagcagattg gattgcttca tttaatactt cttctaataa gacgatcttt
     7081 cctttacgag ttgataattt tttaccacct tgcgtgatca aaccaaatgg aatatggtgc
     7141 atgtcgtcag accagtcaaa gcccatttcc ttcaagacag ctttcaactg tttaaaatga
     7201 tagctttgtt cattccctac tacatataga gattgtttga agtcgtaagt acgtttacgg
     7261 tacaatgcag cagctaagtc acgtgtaatg tatagtgtcg caccgtctga cttcttaatc
     7321 aacgctggat tcaggtcata agcagatagg tcaacgatct ctgcaccttt atcctcgttc
     7381 agcaaatgtt tttcttctaa aagtttgacg atttcatcca tcttgtcatt gtagaaagct
     7441 tcaccgttca acgaatcgaa acgtacttcc agcagatcat agattttatt gaattctttc
     7501 atcgattcat cacggaacca ttgccataat tgaatagcct ctttatcgcc ttcttccaga
     7561 cgtttgaacc aagcacgagc ttcttcattt aattctggtt ctgtttcagc tacttcatgg
     7621 aattggacgt ataaacgaag caattcattg attggttctg ctttaacagc ttcttctgta
     7681 ccccattttt tgtaagcgac gatcagtttt ccaaactgtg ttccccagtc acctaaatgg
     7741 ttgatgcgga ttggctgata accgattttt tccataatga aaccgatcga attaccgata
     7801 actgtcgaac gcaaatgtcc cattgagatt ggtttggcaa tattaggaga agacatgtcg
     7861 attggtactg ttccttgatt cccgatgttg ctatccccat aatgttcttt ttctttcact
     7921 acggtttgca aaactttttt gctgataagt tctttattca tgaaaaagtt caagtaaggt
     7981 ccgacaactt cgattttttc aaagttggca gaatcgattt tttctgctaa atctgcagcg
     8041 atttgctgtg gcgcttttcg gtaaactttt gctaatgaaa aggctggaaa tgcaacatct
     8101 ccgtgttcag ctgattttgg attttctagt aattgctcga cttgttccag tgttaaatca
     8161 tctttgacag cgtcatagac agcttttgcg acaagatctt tattattcat ctttattcct
     8221 cctaaagtgt tcccttgatt gttaaacaaa aaaaatctct agcgacggca tacttagtat
     8281 gccatcacta gagacgagat ttcccgcggt accactccaa ttgtcctaaa aaaggaccag
     8341 ctccatctga tatcggtaga cgaccgtctg tttaaaacag aattctccaa gtgcgcttcg
     8401 ttgtatcttc ttcattcggc ttccaccact tccgaattcg ctagccaaga atgatacaag
     8461 tactctcttg ttcaacaaat cttagtctta ttaaactttc ttgaattata tcagaaaaaa
     8521 aaacgaaagg ctagtcgttt cataaaaaaa aaatccagat ggcctaaagg agaagaaata
     8581 ccacctggat agctataaat catttcgtaa atacgaaaga cttaacaaat taatcagcta
     8641 cgataatcgt tccttcttca gaagctaaaa gattctcgat attttctaaa gaagtgatga
     8701 ttgccttagc gtttggtttt gcttcaacaa atgcaatagc tgcttctact ttcggaagca
     8761 tgcttcctgg agcgaattgc ttttcatcaa tgtattgttt catttctgaa acagtaacag
     8821 tttctaattt cttttgatct ggtttttggt aattgacata cacattgtct actcctgtca
     8881 atacgatcag caaatccgca tcgatgattt cggctaattt ttctgaagca aaatctttat
     8941 cgattacggc ttctcgtcct tctaatccag tagctgtttc tacaacagga atcccaccgc
     9001 caccaacaga gactgtgata actccttgat cgaccagcgt ttcgatgaca cgtgcttctt
     9061 taatcgagat gggtttaggg gaagcgacta cttttctcca tccacgtcca gcatcttctt
     9121 tgaatgtcac agtggagtca gcattcattt gttctttggc ttcttcttcc gtgtagaatg
     9181 gaccgactgg tttacttgga tttttgaatg aaggatcatt ttcatccaca ataacttgag
     9241 tgactaaaga aaccacatct ttatcgatcc ctttttcctt cagtacttcg cccatggcgt
     9301 tttgtaacca gtaaccgata gatccttctg tcattgcaac acatgtatct aaaggcattg
     9361 ccggtgtttt ctcagaatca gcagcttgct gctgaatcaa taggttaccg acttgcggac
     9421 catttccgtg ggagatgatc aattcatctc cttgttcgat aaatttcact aaatactttg
     9481 ctgtttccat taacgcttct tgttgggctt ttgcacttgc atccgtagac aagatggcat
     9541 tgccccctaa agcgacaact acttttcgtt tggccatttc attcgtcctt tcttcagttc
     9601 taccgaattt tctagataac aaaacgagcc agcgttttgt gattttccct caatgctgac
     9661 tcgttttttc gtgagatgaa tgtggttatg cttaaacgcg agggataaac aagttaccta
     9721 atgttgctgc catgatcgct ttgattgagt gcatacgatt ttctgcttgg tcaaattgac
     9781 gagcatattt gctgcggaag acttcatctg ttacttccat ttcagtgatg ccaaagcgtt
     9841 ctttcatttg ttcaccgtaa acagtgtttg tatcatggaa agctggcaag cagtgcaaga
     9901 agatcaaacg atctgtgttg tgtgtttttt ctaccatttc catattgatt tggtaaggtt
     9961 tcaacaattt gatacgttct tcgaatttat cttcttcacc cattgatacc catacatcag
    10021 aataaaggac gtctgctccg tctacacctt tatcaacatc gtcggtaatc atcaattgtg
    10081 caccggattt ttcagcaaag ccttctgcca ttttgacgat ttcttcttca ggttgtaatt
    10141 cttttggtgc aacgatacgc atattcgctc ctaaaattgc acctgtgact aataaagagt
    10201 tagccatatt gtttcgacca tctccgcagt aagcaacagt gataccttct actgttccaa
    10261 agttttcttg gatagttaaa aagtcagcga tcatttgtgt tggatgccat tcatcagtca
    10321 agccattcca aactggaaca ccagagaatt ctgctagttc ctctaccatt ttttgactga
    10381 atccacggaa ttcgattccg tcaaacatac gtcctagtac tttagctgta tcttctgtgg
    10441 attctttttt acctaattgg atatcatttg cacctagata ttcaggatgt gcacctagat
    10501 cgatcgctgc agtagtaaat gcagaacgag tacgtgtaga tgttttttca aataaaagcg
    10561 caatattttt tccttctaga tagtgatgtg ggataccacg ttttttcaag tctttcaagt
    10621 gttctgaaaa atcgatcaaa tattgtaatt cttcttttgt aaagtctttt tctgctaata
    10681 agcttcttcc ttggaatact gattctttca tattaaacat tctcctttag attgttttta
    10741 tttaagatta aagaggaaat cccgacggca gctgacagta agactgccgt caggaaacaa
    10801 attacttcaa gtcttcacgg acaagtggtt ggctcatgca gcgtggacca ccacgacctc
    10861 gtgacaattc acttgaattg atttctaaca ctttgatgcc atagctacga agcaattcgt
    10921 tcgatacata atttcggtta taagtgacta caacacctgg agcgattgcc aatgtgtttg
    10981 atccatcatt ccattgttct cgaggtgcaa cgatctcatc tccattacca gttgggatca
    11041 gtacaagatc atctaatcca agagcagctt tcatcgtttc gtgtaagtta tctgaatgag
    11101 taatcttgat atcatcacca tctggttcga ttgtaaagac atccatctta ccgtctttac
    11161 tttgaattgc tggatggata gtgaatttat cgtgatctac cattgtaaat acggtatcca
    11221 aatgcatcat tgcacggttg ttcggaattt tgattgccaa cactttttca aatcctgaat
    11281 tttttgcgaa taggtggcgt gctaattttt ctaaagcttt cgcattagtt cgttgagaaa
    11341 tcccaacagc tactacttta tcgcttaaga tcaattcgtc tccgccttcg atatgatctg
    11401 gattttcacg atccaaccat acttcgattc ctttatttgc aaatcttgga tgatgtttca
    11461 aaatatattc tgtaaacatc gattcacgac gacgtgcttc aaaagtcatc ttattgactg
    11521 tcatcccatt tcccattgat gcggaagggt cgcgtgtaaa gtacaagttt ggcattgggt
    11581 ccatgtaaaa tggatagtca tcgtctgctg acagatcata tagactgcta gaacgtactt
    11641 cgatatcttt tgtacgaacc ccagccatga ttttatctac catttcttgc gtttccattg
    11701 atagaaggaa ctcatgaaga ccctctctta cagcattcga tgcaatatga gattcatcca
    11761 acattttatt caagaattgt tctttgacat tgcctgcgtc aatcgcttct gccgctaatt
    11821 tttccaagta aagagtctcg acgccttcat tttgcaatgt cttcgcaaag ttgtcatgtt
    11881 cttcttgagc aatcggaaga tatggaatat catcgaacaa cagacgatcc ataatatctg
    11941 gtgtcaaatt ttccacttct tgtccaggcc gtttcagtaa aactgtcttt aatttcccta
    12001 tttcagagaa aacgtgaata ggtttatcca tgttgtgttg cctccttctt tttgattaca
    12061 tgtataggat atcctccttt tgtaatcact ttcataattt tgtctcattc aaaatgagac
    12121 attcctcaca ttaatattcg ataattaaat aattaaacgt ttttctattt ttaagcgtaa
    12181 tttattcatt ttttattctg tatacacttt caaaaaaata tttattcatt gaaaaaattt
    12241 ttttgaataa attaaaaaaa taaattatat tatcgttgtt agcgtttact atatgtatta
    12301 ttgttgtttt ttatacacaa atcattcgct tcaaaacaaa ttattcaata ccattgtata
    12361 ttttttacat aataacaaat tatatttgta aagtattaaa acttgtttct aatgttggta
    12421 tgttaaaact ttacaaattc tttaaaaaaa gtgacaataa atatagtgaa caataaggag
    12481 aggacttttt aatgcgtgtt aataaaatcg attttcactt ttcaaaactc agatatcaac
    12541 ctgattttga acatttctcg gatgaagagt tccaacagat caaggaacat actgttcttc
    12601 gttcttataa aaaaggacag atccttttcg acgatggtga tcccagacaa cggttgtata
    12661 tcttgaccaa aggactcgtt cgtttagaac gttatgatga aagtgcaact tattactact
    12721 atgactatgt gaatacaaat aacctttttc ctatgtcagg aatgtttgga gaagagacct
    12781 acacttatac agctcaagcg atgactgata ttgaaacatt ctaccttcct gtttcctact
    12841 atgaaaaatt agcacgaaaa aatcccgaac agttgatcta tatgatacaa aaaatttcta
    12901 aagtagttga aatacatgaa ttgcgtatcc aaatcggatt gacctctagc gcctttgacc
    12961 gggtaaaaca aaatttattc attttgaaag aagaattggg cgtaccaaca aaagatggtc
    13021 atatcaccat tccttatccg atcaccttaa aagaattagc agtgaatagc ggcacgacac
    13081 gtgaaactgc tggacaagta atcaaacaac tacgtgattc cgggctgtta gattatgaca
    13141 aaaagcgttt tactttctgt aagacagcta ctactccagc tatgagagaa taacgcgaac
    13201 aaaaataagc agtgaccctg ataaat
//
LOCUS       DACECY010000071        13146 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.075, whole genome shotgun sequence.
ACCESSION   DACECY010000071 DACECY010000000
VERSION     DACECY010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.075"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(77..149)
                     /locus_tag="IWO38_002268"
     tRNA            complement(77..149)
                     /locus_tag="IWO38_002268"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="IWO38_002269"
     CDS             530..1099
                     /locus_tag="IWO38_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ0314340.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="IWO38_002270"
     CDS             1086..1946
                     /locus_tag="IWO38_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0314341.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="IWO38_002271"
     CDS             2136..2840
                     /locus_tag="IWO38_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0314342.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="IWO38_002272"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="IWO38_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ0314343.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="IWO38_002273"
     CDS             4677..5993
                     /locus_tag="IWO38_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314344.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="IWO38_002274"
     CDS             5994..6863
                     /locus_tag="IWO38_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314345.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="IWO38_002275"
     CDS             6918..7727
                     /locus_tag="IWO38_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0314346.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="IWO38_002276"
     CDS             complement(7830..8459)
                     /locus_tag="IWO38_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAQ0314347.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="IWO38_002277"
     CDS             8629..9921
                     /locus_tag="IWO38_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0314348.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="IWO38_002278"
     CDS             complement(9997..10659)
                     /locus_tag="IWO38_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0314349.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /locus_tag="IWO38_002279"
     CDS             10884..11168
                     /locus_tag="IWO38_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ0314350.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="IWO38_002280"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="IWO38_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ0314351.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4369 a         2265 c         2833 g         3679 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    13081 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13141 ccattc
//
LOCUS       DACECY010000072        13046 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.076, whole genome shotgun sequence.
ACCESSION   DACECY010000072 DACECY010000000
VERSION     DACECY010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.076"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(61..684)
                     /locus_tag="IWO38_002281"
     CDS             complement(61..684)
                     /locus_tag="IWO38_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314352.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(843..2240)
                     /locus_tag="IWO38_002282"
     CDS             complement(843..2240)
                     /locus_tag="IWO38_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0314353.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(2272..3243)
                     /locus_tag="IWO38_002283"
     CDS             complement(2272..3243)
                     /locus_tag="IWO38_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0314354.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            3617..4042
                     /locus_tag="IWO38_002284"
     CDS             3617..4042
                     /locus_tag="IWO38_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314355.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(4530..5231)
                     /locus_tag="IWO38_002285"
     CDS             complement(4530..5231)
                     /locus_tag="IWO38_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0314356.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(5247..5687)
                     /locus_tag="IWO38_002286"
     CDS             complement(5247..5687)
                     /locus_tag="IWO38_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ0314357.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(5687..6262)
                     /locus_tag="IWO38_002287"
     CDS             complement(5687..6262)
                     /locus_tag="IWO38_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ0314358.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(7110..7589)
                     /locus_tag="IWO38_002288"
                     /pseudo
     CDS             complement(7110..7589)
                     /locus_tag="IWO38_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(7748..8449)
                     /locus_tag="IWO38_002289"
     CDS             complement(7748..8449)
                     /locus_tag="IWO38_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0314359.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(8557..9966)
                     /locus_tag="IWO38_002290"
     CDS             complement(8557..9966)
                     /locus_tag="IWO38_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAQ0314360.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(10014..11879)
                     /locus_tag="IWO38_002291"
     CDS             complement(10014..11879)
                     /locus_tag="IWO38_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0314361.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(12000..12851)
                     /locus_tag="IWO38_002292"
     CDS             complement(12000..12851)
                     /locus_tag="IWO38_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0314362.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
BASE COUNT         3734 a         2809 c         2123 g         4380 t
ORIGIN      
        1 attttgccgt ttgtttctct aatactcagg tcaaaacgct gatttcacaa cctctgtttt
       61 tcattgtcca taggattgat attgtccttg tgatgactca ttcgtaataa ttgaggcttg
      121 attttcttca cttcccgtta tcgtttgtcc actgccagaa tctgaaggac ttgttcctgt
      181 cgactcgttt ttttgagaag agacagttgt tttcttgact tgttcatttg tctttttcca
      241 ttccgctaaa cgatcggcga ccactttttt gtctactttt tcagaataag cggtgatcac
      301 ttttccattt ttcaaacggg ctactttgtc gccatcatga gtcaaaagca aaacggtcaa
      361 atgcttgtct ttaaattgat attgtctgag agaaatcgca ctaagtacgt catcgatatt
      421 aataaattga ttttcttctt ttccttttgg caaataaatg tccaattcgc ctttttcgta
      481 attggcagtt attttatact ccgcccacca ataagttgag aattcttttt tgaaattatc
      541 tactgattct ttgcttgcta cgattttttc tgtttctttc tgagcaagat ctgctgtcat
      601 ttttcctgaa aatgctgcgg ataaaataag cagtaagaaa aaaccgaata accaaaagag
      661 aagaaacggt ctttttttcc tcatgctttc ctccactcct atttaatttt gactattcta
      721 ttatatacaa aaaatgaaat agaaagacaa aaaagttgaa aaagatcgta aataattgaa
      781 cttctttttc acaaaatggt aagaatggag aatttcacgc atttgctctc aactattctt
      841 tcttaagcct caaatccgtt tttatctgat acgtctttga accaatgacc cgattttttg
      901 attgttcttt ttccatcttg tgctaagtct actgaaataa agccatatcg attcttatat
      961 gcattggtcc acgaccagtt atccatacat gtccacatat gatagcctaa acaattggca
     1021 ccttcttgaa tggcttgatg gacatatttc agatgagatt ggacaaattc gatccgataa
     1081 tcatcgtgga tcatgccatc ttctttgata aatcgtgctt cgttttctac acccataccg
     1141 ttttcagaaa taaagcagtc gatatttcca taatgatctc gtacattgat caaaatatca
     1201 tagattcctt gttcatagat ctcccatccg cgataaggat tcattttacg accaggcatt
     1261 tcataattgt cgaaataatc ctcaggcatt ggaccgtttt tagtatcgat tggtgtttct
     1321 tttgctttta cccggcgtgg ctggtaataa ttgataccta aaagatctac tgtattttct
     1381 ttgataaccg tcaaatcctc tgcatggatc acaggcatga gatcaagttc ttttactatc
     1441 tcaatcaatt cacttggaaa ctcgcctttt acagctggat caaggaatga acgattgaag
     1501 aacgcatctg ctatttgcgc tgctttaaca tcttcagggt ttgattcgtc tcttggatag
     1561 cttggtgtca gattcaagat gataccaata acaccacctt gctccatttc atggtaagat
     1621 ttgatggcta acgctgaagc caacgcttca tgatagccaa cttggatcgc ttttttcata
     1681 tctgactctt ctggataatg ccatcgatat aaataacctg cttcgactgg aacgattggt
     1741 tcattatgag taaaccattt ttttactttc gaaccaaaca attcaaaaca acttttggca
     1801 tatttttcat aggcttcaac cgtttctcgg ttcaaccatc cgcctttttc ttgcagatac
     1861 atcggcatat cgaaatgata cagattcaca aagggttcga taccgttttc aatcaactca
     1921 tcaaaataac gatgatagaa acgaacggct tcttcgttga ttgttccaga ttcatcaggt
     1981 atcaaacggc tccattggat agatgttcga aatgaattat gcccagtctg tttcatcaac
     2041 tgtacatcct cttgatactt cgtataaact tgcgaggttt tttcaggtcc tacttgatca
     2101 aagaactttt gcggttcttt tttgtaccaa taatcaaaaa tgttctctcc ttttccatcg
     2161 cctgaaatcc ttccttctgt ttgtgggcca cttgctgcag aaccccacca aaaatttgct
     2221 ggaaattgat attttttcat caaagcacct ctttttcttt tttagttcgt tttagttgat
     2281 tccatgtatg atattttcca tcacaatcac tttttcattt tcttcctctt cttttttgat
     2341 tcgttttaac agctgttctg ctgctcgttc cccgatttta tctgttggtg gcgtgatcgt
     2401 gattttcgga aggatactca gatcgttttt atctaaataa tcatagtgac tgaacgcaat
     2461 atccttcccg attctgacac cttcttgatt gaaacattcg atggcaccaa tcgacatatt
     2521 gtaattgctg acaaaaatag cagttgcccc tttttctaat aatgcttttg ccccgtaata
     2581 gccgcttttt ctagaatagt ctcctgtaaa gacctctaat tggatcgatg gatcattttg
     2641 gaccgcttct ttggctcctg ctaaacgttc tcttgctacg tagtctgttt gagtggcagc
     2701 aataaatccg atttttttat gtccttgttc accatgatga gcgatcactt ttgctacact
     2761 ttctcgatca ttcactaata ttgtatcaat acctgctggt ccttctggac aattcaatgc
     2821 gataactgga atactcattt ttgtgagcat ttcaaatcca gaccactctt cttcggataa
     2881 gaagacaatc agcccatcga tcacatgctt catcaggtat tcaatttttt gttcaatttt
     2941 ttctggctga tcggcaaacc cgcttaataa taagctatac ccattttctt cagctactct
     3001 ttcgatccct gagacaactt ccgacccaaa acggctggaa atcccgttta tcaaaagtcc
     3061 aatagagaag cttcgattat tcttcaaccc tttagcaatg atgttttctt tatagtccaa
     3121 cgctgaaatc gcttctgcta tttttttcat gttcttttcc ttcagttgct gaccattcaa
     3181 gtaacgggaa actgttccca ctgaaacacc agccatctta gccacatcac ggatcgatgc
     3241 catacattct cactcctgcc agttacattt gatttctctt tcttgttgtt cgattatgat
     3301 gaccgcattc tttgcatagt gttgcttgtc atcaatatcc atataactaa agtcttcagt
     3361 agagcaactt cctctcacta ttaattatac ttaaacaagt atttgaaccg gttcattttt
     3421 aatatagcat ttttttaaaa gattgcaaca cttttttaaa acgctttctg aaagtggcgg
     3481 attttttgcc tttttaaaat tttattctaa aatttactct atttgtgggt gttttgtgcg
     3541 tttttctctt ccatccgttt gaagttttac attccaatgg tataatttct ttatcctatc
     3601 aaatggggga agtattttga agaaattaat cgtactattt attttgtctg cgggactttt
     3661 gacattgact ggctgtgatt tgtttggcgg ctctgaccaa tcctcggacg aaacaaccga
     3721 ttcttctgtc ttaaaagcgg aaaaagcaga taccaccaac agtgtatata cgctaaaagt
     3781 acgtaaagat atggatttat ccgcgggaca gttgacattt acagataaaa aaatggaatg
     3841 gacacgcacg tatcagacac aaggaactga ttcttctgaa tcatctgaag aaaaaacagt
     3901 gctgacagat atcaagatca ctaccaaaga tgacgaatat attatttcag gaaaagaaaa
     3961 taaaaaagaa acaacagtca tatttacaaa aatcggtaat taccgtttga aggatgagga
     4021 tggcaacatc tacagtctat agtgccgtct gttggatctt cttttgtttc tccccttctt
     4081 ttctgctata gtaaagaagc aagatgattg taaaggagac gaaatggatg atcgatagtc
     4141 ctgattattg tacatgtaat aacaacaaaa atatccatac agaatttaat gaccaaggaa
     4201 gcttgagcat atgcgacaac tgtcacaaac tagtcaaaga ttcatttaaa acatttgaat
     4261 cttatcctgt tcaaaattat gtaaaaagta gaagaagata aacaaatcca agcaaaactc
     4321 actatggctg atttttatga acttcaaact cagcttcttc ctttgatcaa gcttcatcag
     4381 taaaacaaac agtggatcat ttcagtttac aaatcaaaga gtaaagattt atttatttca
     4441 aatatttgaa tataaaataa ccaaacgata gaaaggctta ttctacatca cgtagtaatt
     4501 tttctttccc gtcgtttggt tttttatttt tagcctaatt cttgaccttt tgcatgctgg
     4561 ttatccgtaa aatattttcc agcaggcttt ccttccaatt cagctaattt ttcttggatc
     4621 tgacggatca ccttttttgc atcttctgga ttttctacaa aatcgaattt atccccatcc
     4681 acttgtattt taggacatac atcaaaatct tcataccatt gattatagcg ggtgttcaac
     4741 gtttcataat aggtatataa cgtctcatcg ctttctagct gttcatagtc acgcccacgt
     4801 tttttgatac gttctagcat cgtgtccaaa gaaactttga tatggaccat caaatcaggc
     4861 cgtttttttg gagcaacatc atctaactcg tttagcatcg tatctaataa gctatcgtac
     4921 tgctgtactt cgatatccgt tacccgacct aaatcagcgt ttagatggaa caacaggcta
     4981 tcttcataga tggatcggtc tagcacattg tcatcgttca ctaaagcatt tttcattgct
     5041 aaaaatcgct tgttcaaaaa gaagatttgc aataaaaaag cgtactgctc aggatttgaa
     5101 taaaagagcg gcaatacttc gttatcttcg acattttcat aaaatggttt agaatcaatc
     5161 tcttttgcaa gcatatccgt caaactgctc ttacctgcac caatcgttcc tgaaatcaaa
     5221 atcactgaca tacaaccctt cctcttctat aagtactcgc ctacgtaagt ttcaacaggc
     5281 aatgtttcaa aatcatattc acgtacagct tggtctgatt ttaaatacgt atggagactt
     5341 tgtgtaatca tcaagttcgt aggtaccgtc tcttcttgta caatgatcat cggttttttc
     5401 aacatagttg ccacacctaa ttcgtaagcc gttcctggat cgattgtttg atgctcaaag
     5461 tcaaggatag caacaacaaa ttctgcattt gtcagttctt ccatatcttt ttcgtatact
     5521 tcctgcgccc atccagcgct gaatagttca taattgcttt cttggtgatg acgtggagag
     5581 tagaaacttg tgactgtttt gttttcctcc aacgcttttt cgatacgact tactcgatcg
     5641 atttgctcct ctgaaaaaaa tggtcctgct aaatatattt gactcattat tttgcctcct
     5701 tcaataataa ctgattttta atagatactg ctcgtaattc attgaccagt tccaatggat
     5761 tgttggaaaa gattacttga tccaaccatt ttttatccat aaaatcctct tttgcaaaag
     5821 tttctaataa ttgttgcaag ccgctgtaat aaccgccaat attcaatatt ccgattggct
     5881 tgtcgggcgt cagaccgatt gctatccaag aaagaagttg tgagaattcc tccaatgtac
     5941 ctagacctcc agggaaaacg aggacagcat ctcccttttc aaataataat acctttcttt
     6001 catcgatcgt ttctgtagga ataaatgtag taacatcttt tccaggcaat tcagtttcaa
     6061 aaagcccatt cggatagata cctgtgactt ttccgttatt ttccagtact gttttactaa
     6121 tcactcccat aatcccggag tatgaaccac cgtaaaccag ttcgatttct tcttgtgcca
     6181 tgtattttcc taacgtttgc gcaataaatt tgtaactttc tttattacca aaacgggaac
     6241 cgcaaaaaac agtaagtttc ataaaaaaac actccctaac tcaaaagtct atcttcattc
     6301 tacacgtgtt tttttaaaat aaaagactag attttgtagt tttttagtgg tatataggat
     6361 agacgccact atatatagta tttagaaaaa aatcattttt cagccatttt atagataaaa
     6421 caaaaataaa taacgtgttt tcatcgatat gaaatgactt acttatagaa gatactggga
     6481 tttcgtccta tatgccaata acgtgtatta ttttaaacaa caaatacttg tttcttttat
     6541 taacgaaaaa atcattttgt tctcgtgtta tgtttcactg ataaatgatg tttctctaaa
     6601 taaaaaagcc gacgaactaa acgatcaaaa tttgatcatt tttgttcatc agcttctcct
     6661 aaaaatcttt ttgttaacga taaatagctg gatttgtaaa aatatcactt gaatcgtcac
     6721 tgctggaaga aaattcagtg ataaccgcac ctgttgcgcc tgcctggaac tagtgtttga
     6781 tattgggctc gaacgtgtac tgatctctag catgaagaat aatttctttt tttactgtat
     6841 aataactttc tttccttttt ggaatagtac aggaaagttt ttttgtttcc tttccttcga
     6901 cataaggtat accagaccct ttcgacaacg aaaggttctc atttttcctt catcgtcatt
     6961 tcaaggaggg tgccgatgct ctggacagtt tcgatcgctc ctaaaccaaa atctgtatat
     7021 tcgattcttt ctaactctga ttctttcata ccattcctgt ctcaaggtac atctattgta
     7081 cttttttctt gtaatccatt ctgcctctcc taactaattg tcaaatcatc ccaagatatg
     7141 tcttcttcct ctttttcttc ttggatgatt tctgcattcg gatctactgg ttttttcaat
     7201 aataaaatgg ttattcctgt gacaaaagaa ccagctaaca atgcaccaat gaatgcccat
     7261 ggtctggtca tcgtagcgac tgcaaacatt cccgatgccg gaatacggct atctgcgccc
     7321 caaaacatgc ttaatccgcc acctactgtc ccaccgactc ccgttgctat gacattgcgt
     7381 gccaagtcgt tcaatacgat agggaaacat ccctctgtga tctgacagat ccccatagga
     7441 aaagcggtct tcaatgtatc gatttcttga cgattgagta tatttttacg aatgattttc
     7501 cctaagaaat aagcgatcgt caagccgaat ggggttacca tcgaagctaa aatcaataca
     7561 gtgattggtt catagattcc ttctgaaaat cgtgccacac ccccttatcc agaataaatg
     7621 gtaatccaga aacagccgtg tatctgcggt aataagccta accaaacgaa aaatatgaga
     7681 agctattttt gtttggctgt tcttattact tgatgcaggg cagctttttc acaacctctt
     7741 tttttattta tgtttcataa tcaatatggt aaggatgcag ctccgtatgg tagaccgcct
     7801 ccgaatattc aatcatttcc ccaatttgcg tagacgattt ccgtatccgt ttgatgccga
     7861 tctgtgcgtc tgttttcaga attccttgtt cttcttctgt taaaaatact gccgagaaat
     7921 catcagaaaa tgagtcaata gtatatcctt ttttcgtcag caaacgatac aaagattctt
     7981 tgccaaattc ttctctgctg aattgcatca tttcttgtgg aagataatga acaaaataaa
     8041 tataaggctg attgttcaaa taatataagc gcttaaaaca aacagcttgc tcaccgaaaa
     8101 aggaaaacaa aggatcttcc ttctttatat ggaccaattg caaatccagc gtttctttcc
     8161 ggacttcata tcgggagttc tccaagattt gcgtaaagct tgccgctttg gataatcgat
     8221 tgtaaggacg attgcttaga accgtcgtcc cacgaccact tttctttgcg acatattcat
     8281 ctgatgccag cagttcgatt gcctttcgga cagtgatttt gctgacttga aacagttctt
     8341 ctaattcact ttctgtcggc aacatcgaac cgactggata ttttcctgag aaaatatctt
     8401 ctttaatttt atctgcgact tccaagtata gtacacgtgt ccttttcata acgtcaccct
     8461 ttgctttact attactaaaa gaatagcaca taaaaaataa aaaaagaaca caaatcacat
     8521 gtgagaagtg tgttcttttt cattcagctg ctttaattac aagtttacgc catttgaggc
     8581 aattacttct ttataccaat caaaagattt tttctttgat cgattcagtg tcccatttcc
     8641 ttcatcgtct agatccacat agataaagcc gtaacgttta ctcatttctc ctgttgatgc
     8701 actgacaaga tcgatacatc cccaaggcgt atagcccatc aaatctacac cgtctgctac
     8761 tgcattagcc atcgcttcga tatgacgacg taaataatcg atccgataat catcaataat
     8821 cgtgccatcc tcttccatat gatcaatggc tccaagaccg ttttcaacaa tgaataatgg
     8881 tttctggtaa cgattgtata attcattcaa tgcgattctc agcccttctg gatcgatctg
     8941 ccagccccat tcacttgcct caagaaacgg attttttact cctcccagca agttcccttc
     9001 tgaagttgct gcttgtggat cagtctcatt gattgccgta gacatataat aactaaatcc
     9061 gatgtagtcc actggatatt cttttagcaa ctggaagtct ttttccgttt gctctaattt
     9121 ttccggatcg actccatatt ttttatgggt acgcgctgtg tatgcagggt attctccacg
     9181 tacttggaca tccgtgcaga aaaaattgaa ttcttggttt tggatcattg ttgctgcctg
     9241 attgactgga ttggcatcga tgctgtaagt tgttgcatag ataatcatgc agccgatttg
     9301 gatatccgaa cgcaattcgt ggccgatttt tacggctttg ctacttgcaa cgaattgatt
     9361 atgccatgct tggaaaatat tttggtactc tcctgctcca ttgcttttta ctaatccttg
     9421 acttaaagct ggaaagtgaa aggccgaatt gatttcattg aaagtcatcc aatatttgac
     9481 tttactgctg tatctctcta atactgtctg agcaaatcgt tcatagaagt cgatcagttt
     9541 tcgatttttc cagccaccgt attcttttgc caaatgaaga ggcatttcgt aatgagaaat
     9601 cgtgatcaca ggttcaatat catattttaa gcactcatca atcagctgat cataaaattc
     9661 taatcccgct tcgttaggag taccttcgtc tccatttgga aaaattcgtg tccaggcaat
     9721 cgaaaaacga taacatttga atcccatttc cgcaaacaag gcaagatctt ctttgaaacg
     9781 atcgtaatga tcgattcctc gatgattcgg gtaaatatat ttttcttgat caatcgtcca
     9841 gtcaaattct tcactgccga taatcgcaaa gcgctgctta cctccaggca tagcatctgc
     9901 aacggatagt ccttttccgt ctcgatcata agccccttct aattgatttg gcagcagttg
     9961 ctccacccca taaaaatttt tctgaaaaac gtgattgcat cctgatttcc tccttatttt
    10021 acaattgcca gaacatcttc gtttgcaata atctcttttt ggtctaattc aagtatatcg
    10081 gcaaatgcat cagtattcgt aatgacaact ggagtgatta cgctatagcc agctgctcta
    10141 attgcttcga tgtcaaaacg aatcaataaa tctcctgctt tgacgagatc cccttgtttg
    10201 actaagatct caaagccatt cccatttaat tctactgtat ccatcccgat atgaatcaaa
    10261 atctccgctc cgtcagtcgt tgtgatccct acagcatgtc cagttggaaa aatagtcgtg
    10321 atctctccgt ctgcaggtgc aactagttct cctacttctg gatcaatagc gatacctttt
    10381 cccatcacgc cagaagaaaa aacttggtct ggtacttcac tcaatggcag tactttaccc
    10441 gttaaaggac tttttaaggt atgccgtgct gaagtaatcg gttctcctgc attcgcatgt
    10501 ttgttttcta attgattgtc ttgtgtttgt tcgtcaaatc ctaaaatcaa tgtacctacg
    10561 aatgcaagaa caaaagcaat tcctgtagct attactgcta ctgtcacatt tgactctaca
    10621 ccatccaccg tattaatgaa agtcggaatc gtcaataagc tgaccaaact gctggagaaa
    10681 gcttttactc cggaaaatcc gataatagct ccaccaatac ctccagaaat acaagcggca
    10741 atgaatggtt tcttcaaagg taatgtcaca ccatacacag ttggttcagt gattccaaat
    10801 aacgaagtca ctgtagaaga aatccctaat gctcgaagtt tcgtatcttt tgttcgcaat
    10861 gctacagcta aagcagcccc accttgtgcc aagatggctg gaagaagcat tggcatcaat
    10921 acatcaaacc catattgttc gatattcaaa aacatgatcg gtacgaatcc ccaatgcatg
    10981 ccgaacatca cgaatacttg ccataaactt cccatgatta gacctgctac gattggacta
    11041 aatccataaa tcgagttgaa gagaccgcct aaagcattac cggcaaccgt cccgattggt
    11101 cctaaagcaa gaaatgttaa tggaaccatc actactaaaa cgacaagcgg aactaaaatc
    11161 atttgcagaa attgcggaat cacttttttt acgaatggct ctaatttact ttgaacccat
    11221 acggctagaa tgattggtat gacagaagaa gtataaccag atggtgaaag aatcactggg
    11281 atcccaaaga aactgatagc ttctcccgca ccagcaactg ctgtgatagc tggatacaca
    11341 agtgccatag caattgctac tgctaaaaac tggttggctt tgaacttttt agctgctgta
    11401 aaagcaagca tgacaggtaa gaaggtaaag acaccatctg ctgcagcaaa caatatttga
    11461 taagtaccgc tatcagctgt cagccagccc atcaccgttg ccaatgataa gaatcctttc
    11521 aataccccgg cggctgccat cgcccctaga aaaggtgtaa atatactaga gataatatcg
    11581 atcagacgat tcaaaatatt tttggactct tcttttccat cctccgaaac tgcaagacct
    11641 tcctcatcca cgatcgattg ataaacatct gctacattac tgccaatcac tacttgatac
    11701 tgaccgccac tttgtacgac ttggatcacg tccgggtctt cttttaactt ttgcgtatct
    11761 gctaaagatt catcttttaa tctgaatcgc aaccgcgtag cacaatgtac taaacttttg
    11821 acattctttt gtcctcctac cgcttcccat acacgacgac caactgcttg attatccatt
    11881 gttttttcct tctttctttt ttatttaaaa aataaaaacc cagattgaaa aaaacagtca
    11941 caaacattcg tgaacctatt ttttcaatcc aggttttgcc tgccatacag taacaatcct
    12001 tatgattgga tgcttttttt ctcacttact actctttgaa tatgaatcgt taaataaatc
    12061 tgctctgcga tccccatttc ctgtttttgc gtttgtcgaa gatactcatt gatccgttcg
    12121 acacaatgaa acgcttttgg atactttcct tgaactaatg cgtagagaaa ttcatcttgc
    12181 tcgtcagatc cttccccttt gagataacgc tgggcaaaaa actgcagatg agtaatgata
    12241 cgctgataat tcaaagattc ttcatcgaac acgatgccga agaatttact gatgatactc
    12301 agtatatctc ttaccattgt ggtagcggtc atcgtcactt gcatgtcttc gtttccttgt
    12361 tggctattca caaaatgaaa ggcaataaaa ccggcttcat cctcagaaag ttccacaccc
    12421 gtcttttgtt tcacgaacaa aagagcctct ttcgctgctt gatattcttt cggataaaat
    12481 ttttttgttt caaataataa tggattaggc agttggatat tttcttttgc tcgtgtgatc
    12541 gcataatgaa gatgatcggt caatgtcaga taaatattag gcgaaaattc aacttttagt
    12601 ttatcttcag ccagttcgac tacttgtttg acccattcga tctcctctgc cggaatatca
    12661 gccatcagct gctggaattg gtttttagca aaagaatctc ttaaaacaaa ttctttttgg
    12721 accgcttctg gcgaaatctc gtccccaatc tttttttgaa aagctagtcc ttttcccata
    12781 caaacgactt cttccccgaa tccatttttc gaaatgacga cattgttatt caaaatttta
    12841 tcaattttca ttttgcttcg ccctctcaag aaaaataaaa aaacctagaa ttcgcctact
    12901 gtttcttagc tgaattctgg ttttgcctgc cgtacagtaa caatccattt cttattcaaa
    12961 atcatcatac catcgaattt ttatttatgc aagccttttc atataaaaaa taaaaaaact
    13021 ggaacacaag tctatctaga aaacaa
//
LOCUS       DACECY010000073        12270 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.078, whole genome shotgun sequence.
ACCESSION   DACECY010000073 DACECY010000000
VERSION     DACECY010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.078"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..573)
                     /locus_tag="IWO38_002293"
     CDS             complement(<1..573)
                     /locus_tag="IWO38_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0314363.1"
                     /translation="MDQAKSLETACKLLRLSHVLPTLVRKDLDYSFETKEDWLMYLLE
                     KELACREELKTARLLKQAKFPNKRTLEDYEWHDHICLPNATTKDELLSLSFIHQKENA
                     VFVGTPGTGKTHLACGLGQLAAKSGIDTRFWRVSDLVNELEHHWKNNTLEVFKHRFDK
                     VKLIILDEMGYVPFTKEGAELLFQLISDWYE"
     gene            complement(573..1997)
                     /locus_tag="IWO38_002294"
     CDS             complement(573..1997)
                     /locus_tag="IWO38_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAQ0314364.1"
                     /translation="MLTMTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLL
                     PQPKARKKTGMMYTEKWGGIVSLWLSEDAKLPKKKRRTAEAMTTALKADGFPGSYRTV
                     CVFIQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPF
                     SNAGFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVTQTKTKTEQAVLTD
                     GFLRFSMHYGFETQVCNPRSGNEKGSMENKVGYVRYNFFSSTPIMKDFASFNEALAVE
                     LSHDRERIHYEKHVIINDLWNTEKTDLLALPDTDYPVFKEIEVKVNKYNEIKLDNERI
                     HVPRVRNYSIIYGLLRFDSYQLISTDGEIISEGLRPYMMKKRAIDWQAILPDWRRKSR
                     AMHYSRYWKYLPERIKLFLSHPDWKEQEKRLNQISGLLVTHDMLGIDTHFYDMFSAST
                     TTDAYDIDWHQYDAFLSLTGEVID"
     gene            complement(2392..3234)
                     /locus_tag="IWO38_002295"
     CDS             complement(2392..3234)
                     /locus_tag="IWO38_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0314365.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            3559..3975
                     /locus_tag="IWO38_002296"
     CDS             3559..3975
                     /locus_tag="IWO38_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0314366.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIRESQQSVIDCRKLLVEQLTGGDDL"
     gene            3972..4442
                     /locus_tag="IWO38_002297"
     CDS             3972..4442
                     /locus_tag="IWO38_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0314367.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            4456..5229
                     /locus_tag="IWO38_002298"
     CDS             4456..5229
                     /locus_tag="IWO38_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0314368.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            5243..6055
                     /locus_tag="IWO38_002299"
     CDS             5243..6055
                     /locus_tag="IWO38_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0314369.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            6109..7074
                     /locus_tag="IWO38_002300"
     CDS             6109..7074
                     /locus_tag="IWO38_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0314370.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            7211..9934
                     /locus_tag="IWO38_002301"
     CDS             7211..9934
                     /locus_tag="IWO38_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0314371.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9947..10579
                     /locus_tag="IWO38_002302"
     CDS             9947..10579
                     /locus_tag="IWO38_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0314372.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            11478..>12270
                     /locus_tag="IWO38_002303"
     CDS             11478..>12270
                     /locus_tag="IWO38_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0314373.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT         3977 a         2026 c         2378 g         3889 t
ORIGIN      
        1 ttcataccaa tcgctgatca attggaaaag cagttccgcg ccttctttag tgaaaggcac
       61 ataacccatc tcatcaagaa tgattaattt tactttatcg aagcgatgct tgaagacctc
      121 taaggtattg tttttccaat ggtgttccaa ttcgttgact aaatctgata cccgccaaaa
      181 gcgtgtatcg atacctgatt tggctgccag ctgacccaat ccacaagcta gatgtgtttt
      241 accggtaccg ggtgtaccca caaatacagc attttctttc tgatgaataa acgatagact
      301 caataattca tctttagtcg tcgcatttgg caaacatata tgatcatgcc attcgtaatc
      361 ctctaaggta cgtttatttg ggaactttgc ttgttttagg agtcgggcag ttttcaattc
      421 ttcacggcaa gccaactcct tttccagaag atacatcaac caatcttctt tggtttcaaa
      481 gctgtaatct aagtctttgc gaaccaaagt gggaagaaca tgggaaagtc tcagcaactt
      541 acaggcagtt tctaaacttt ttgcttgatc cactagtcaa tgacctcccc tgttagagac
      601 aagaaagcat cgtattggtg ccaatcaata tcatacgcat cggtagtagt cgaggcacta
      661 aacatgtcgt aaaaatgtgt atcaatccct aacatatcat gtgtcaccaa taaacctgag
      721 atttgattta aacgcttctc ctgttctttc caatctggat gactgagaaa taacttgatt
      781 cgttcgggca aatacttcca atagcgagag taatgcatgg cacgcgattt tctgcgccaa
      841 tcaggaagaa ttgcttgcca atcgattgct cgttttttca tcatataagg acgaagtcct
      901 tctgaaataa tctcaccatc agtgctgatt agctgatagg aatcaaaacg aagcagccca
      961 taaataattg aataattgcg tacacgtgga acgtggatcc gttcgttatc taatttaatt
     1021 tcgttgtact tattgacttt gacttcgatt tctttaaata ctgggtaatc ggtgtccggc
     1081 aaagctagca aatctgtttt ttctgtgttc catagatcat tgataatgac atgtttttcg
     1141 taatggattc gctctcgatc gtgtgacagc tccaccgcta atgcctcgtt gaaactggcg
     1201 aaatccttca taatcggtgt agatgaaaag aagttgtacc gtacatagcc gactttgttt
     1261 tccatactac ctttctcgtt tccgctacgt ggattgcaca cctgtgtctc aaaaccatag
     1321 tgcatagaaa atcggaggaa accgtcagtc agcaccgctt gttcagtctt tgtttttgtc
     1381 tgagtaacgg ccgttgacat attatcaatc cgtattttcc gaggaacgcc acctgattgg
     1441 ctgaagagtt ctttcatgcc ttccaacaaa cattcttgat tctcggcggg gagtacaacg
     1501 gcgaatcctg cattactaaa agggaaagtc atcactaata ccttcacatc tttaaacgct
     1561 ccatcgtgac aaacttccat cgtaccaaaa tcaagttgcg cttctgctgg cgggtgttca
     1621 agacgttcaa agccttggtc ttccagtgtt tccaagtagt gtgtcgcttt ccaatcttga
     1681 atgaatacac agactgttcg gtaggatcct ggaaagccat cggcttttaa agcagtagtc
     1741 attgcttcag cagtcctacg ctttttcttt ggcagcttag catcttctga aagccatagg
     1801 gaaacgatgc ctccccactt ttctgtatac atcataccag tctttttgcg tgctttaggt
     1861 tgcggcagca aatcatcatc tgcatatttt ttagccgtgc gccaatttac atctagctcc
     1921 tttgcaatat gattaatgga tttatcatga ttatttcgta aatttctgat agtattaata
     1981 tcggtcattg tgagcatacc tttcttacct ccagttacag tgtcgagact ataactttag
     2041 aggaatttca ggttgcccgc aatggctttt tatcttaact taatcgattt gcggagttgt
     2101 gtagtttttc gttgcacttg tatgtacttt tattctgcca taaacagctt aatgaatgtc
     2161 cacttgaaaa agtgaataaa cgaaatagat gactcgattt aataaggaag taaacccaca
     2221 cagaaatata ggtgattttc cttgaatgac caagatattg aaagttagtg aaaaaacaca
     2281 aaaaggtagc cctattcaca gaaggtctgc cttttgtgtt atcgccagca aacattagag
     2341 gggatatttc atatagcttt tgtcctctcg acagtcctat cagtttctct tttaacttat
     2401 ctctaagtgc tgtccagttt taatagcttc tagcttgttt cctaactgtg tttttaatat
     2461 gttgaaggct tttgaacctg tgcagtggca agtaaggatt ttttctactc gtgagtcatt
     2521 caatttattt ccaattttca ttacctcttg ctcaggtaga cctaaattat ttatataagg
     2581 tatgccaatc atatggaagc caccgattag gtattttatg ggtttagtcg gaaatgtttc
     2641 aagagcaatt tccatcatat cgataatgcc atgatgacta cagccagtaa agacaatcag
     2701 accgtcttct tgctcaatta cgaccaataa ttcatctgaa aaatcatcat aaacaaattt
     2761 atcatccatc tttttgtata aattcttggt ggtcgagttt gctttgccta gggggatgat
     2821 atggaagctc ggagcaagtt ctgttttcgt ttttattgta tgtaaattct catgcatttt
     2881 aaggatttca tttttaggaa taccaacata cagaaacgtg gttaaaatct ttaaataatg
     2941 gggtaaagtt acaccttcag ctgtataaat aggggcgttt cctgatttta aatatcttaa
     3001 cccgccggtg tggtcagaat gaccgtgtga aataatcact gcctccagat cattcaaagt
     3061 ttcccccatt ttagtcgcat tatagactaa ttttttatta gcgccagcat cgaagagata
     3121 tttcttccct gagtagttaa ttaagagaga taacccatgt ccacttttaa agttcaaatt
     3181 tattcttgag ttttcaaata aaaccttgat ttccactata acatctcctt tcacatgaac
     3241 gtgtattcat atattgtagc acattaagtt ctattttttg ttgtaattac cgaaaaaaat
     3301 attaatggac gctttttacg ctttatttcg tatgtggaag aaaatttctt aggatgtggc
     3361 gggggattgg ggcaaagcta aattccaatt acagtgatca tttaattaga cacctttaaa
     3421 tacccacact ttcatcaaca ctataattta gggaaagaaa agtgttggca tgatatagaa
     3481 aaacctttaa ttgacagcgt ttcctaaaat tggcacgcat cttgcttata gaaaagcgta
     3541 aggataggag gaatgaaaat ggcaactata attcttgcca gccatgggga atttgctcaa
     3601 ggattaaagc aaacagcgac aatgattatt ggagatagtg taccaattca tgccttgtca
     3661 gcatttagag atgaagatga atctattttg gtacaaataa aaaaactttt agccacaata
     3721 gatatagaag aaacctacat tttaactgat attctaggtg gtagtgtgaa taatgatatg
     3781 ctcactatta ttaaagagta tgagggattg caattaatta ctggaatgaa cttaccttta
     3841 gttatttcta ttgcaaccta taatggaaaa attggggaaa gtgagctaga aaagcttatt
     3901 agagagagtc aacaatcagt gattgattgt agaaaattat tagtagaaca acttactgga
     3961 ggagatgacc tatgattaaa ttagtacgta ttgaccatcg tttgttgcac ggacaggtag
     4021 tattttcttg gagtaaatca ttaaatatca atcgtattat tattgccaat aaagaggcag
     4081 cgaatgatga attcaaaaaa atgtctttga atttatctaa accgactggc attaaattaa
     4141 caattttttc agttgaacag ttactagaaa aaatgcctaa aatcgaaaca cttaacgaaa
     4201 atatcatgct tatttttggt aatacaaaag aaattcgtca attttgtgaa gcctatccta
     4261 aagtacaaga aatcaactat ggagggataa tcaaaaaaga aggttccaag caatttagta
     4321 atgccatttt cttgaatgaa tctgagcaag aagatgccaa aaaattaaaa gctcttggca
     4381 ttaaacaatt tattcaacag gtaccaactt ctaaaaaaga agatttgaat acaatgttat
     4441 aaaggaggaa tgaaaatgct cgttcagtca attggtttag ggttaatcgg tgttttttgt
     4501 attttagact caaggctttt aggaagaatg aattttgaac gaccgttgat tgtcagtacg
     4561 cttgttggtt tggtacttgg agacatggaa aaaggattaa ttgttggagc ctctttggaa
     4621 ttgatgtcct taggattagt aaatatcgga gctgctgcac cacctgatat gaatatggca
     4681 tcaattattg ctacagcctt tgccatttta tcaaatgcga acgcagaagc agcattaacg
     4741 attgccattc caatttcagt gttaggtcaa atactaggta tcttaatgcg gacgattttg
     4801 tcaaatttga ctcacgttgc ggatcgcttt atcgaacaag ggaaatttag ccaagcaagg
     4861 agaattcata tactttatgg ctcgggatta tatgctctca tgtattttat tcctattttt
     4921 ctagcaattt attttggaac gaatcttgtt tctcagattg ttgatgcgat tcctgagtgg
     4981 ctaacaaatg gcttaacggt tgcaagtaaa attttaccag catatgggtt tgcgttgtta
     5041 ttacaaacga tgttaacgaa aaaaatggtg ccatttttat tacttggatt tttaattact
     5101 gcttattctg gattgggaat cattggtatt gcagccttcg catgtattgt tgcgtttgtc
     5161 atggttcaag ttcaaggtaa aaactcaaat ggtaaccaaa atgagccaga tccattagat
     5221 gacttataag gaggtagatt aagtgaaaga gaaagatcaa aatgcatgga aatatcggca
     5281 gttcttatgg cgttcatggg caattcaagc ctcatggaac tatgaacgac aaatgaacat
     5341 gggttttatg tacggaattg caccaattct agataacatt tacaagaatc cagaagatga
     5401 agcaatgaag aaagaagctt acgagcggca tatgatgtac tacaactgta cccctcaaac
     5461 aagtgcattt gtgttagggt taactgcttc tatggaggag caatactacg aagaccaaga
     5521 aaactttaat ccggaatcaa tcagtgcagt gaagacttct ctgatgggac cgctatctgg
     5581 tgttggtgat tctttctttc aaggaacgat tcgggtatta gcctttggac taggaattaa
     5641 tttagcccaa caaggtagta ttttaggtcc aattctagca atgttaattt cctttgtacc
     5701 ttccttttta gttacttact atggtgggaa aattggctat aacatgggga ataaatactt
     5761 gtctaaactg tatcaagaag gtttgatgga taaagtaatg tatgtttgct ccattgtggg
     5821 gttgatggtc attggttcga tgattgccag catgatcggg attacaacac caattcaaat
     5881 gggtaaagct tttgtcttac aagatgtatt agatagtatt atgccacaat ttttaccatt
     5941 aggattaacc ttcttcatgt actggttatt gcagaaaaag gtcaaaacag gatggatgtt
     6001 ggcaatctgt ttagtcggag gaattatttt tagtttgtta ggtatcttac aataagggaa
     6061 taaccaataa atttggttga ataatataaa taaggaggaa ttcaattgat gaattcaaaa
     6121 gaaattgtta aagaaatcgt agcgaaaaaa gaggtaaaat caatcgtatt tgttggctgt
     6181 ggcgcttcaa aagcggacct ttatcctgca aagtattttc tagaccaaaa tgcgaagcag
     6241 ttacggatta gtcattacac agccagcgaa tttaactatg caacaccagc atctgtagat
     6301 gagtcaactg tcgttatttc agcttcttta ggaggtacga ctccagaaac agttgaagct
     6361 aacgcagtag ctaaagaaaa aggtgctaca gttattagtc taacacgaag cattgactca
     6421 ccgttaacaa aagatgcaga ttatgtaatt tatcatcgat ttgcagagaa ctatgctgct
     6481 aaattagaaa aaacaggcta ctgtttagaa ttggcagtag aacttttaca tcaagtagaa
     6541 ggttatgacc attatgaagc aatgcaagaa ggatttgcta ctatctatga tcttgcccaa
     6601 aaaagtgcag aatcagctcg taaagatgcg aagaaatttg ctgaacgctt taaagatgaa
     6661 aaagttattt atgtaatgag tagtggtgct acacatgaag tagcatactc aacatcaatt
     6721 tgcttgatga tggaaatgca atggattaat tctggtacct tccattcagg agaatttttc
     6781 cacggaccat tcgaaattgt ggataaagac gtaccgttta tattattaat gaatgatgga
     6841 aaaactagaa atatagatgc tcgagcatta actttccttg aacgctttga tacgaaaaca
     6901 gaagttgtag atgccttgga ttatggcttg tcttcacata ttgctaaaga agtcttagac
     6961 tactttaacc cattcgtgat tactgcagta tttagagttt atgcagaaga attgtcagaa
     7021 gcaagagaac atccattaac taagagacgt tatatgtgga aactaactta ttaaaaaata
     7081 gatcaaacta aataaggtgg cttgagaaat taagccacct tgttttcttc aatgtgattt
     7141 tatacatatg gtaaaataac tagtacatat ggtaaaataa ctagtacata ttgtgaaagc
     7201 aggtggcaag atgcagcgat taaatgacta tctttatcaa ctaacattgc ttttgaaatc
     7261 tacgccttca gataattatt cagctcgcag tatttcgcaa gctttacagt tgaatagaag
     7321 tacaatcagt agctacttaa atgaaggtgt tcgtgaaggg cttttcataa aagtaaaaag
     7381 ttacccggtt ttatttttac atagaacagc tcttgaagaa ctacatatca cactgaataa
     7441 ctccgaaata gaatcaattg aaagcttgtt aacaatttct caaaaacccg cattagatca
     7501 agtaattggc tctaaaggaa gtttgaaaga agcaattgat caaattaaaa cggcagtctt
     7561 atatcctgga aaagggttgc ctttattatt gatcggcgct agtggatcag ggaaaacctt
     7621 tctagcgaat aaaatatatg aatatgcagt agaagaaaag gtcattcaca catcggcacc
     7681 tttcatagat tataattgtg cacaatatgt gagcaatcca gagttactat cctctgcttt
     7741 atttggctac acgaaaggag catttaccgg agcaagccag gagcatactg gtttgctaga
     7801 aaaagccgat ggaggagtat tgtttttaga tgaagtgcat cgcttatctg aagaagggca
     7861 agagaaatta tttacattta tggatacggg cgaattctct cctatggggg ataatagtat
     7921 tcggaaaaaa gccgatgttc gattagtctt tgcaacaaca gaaaatatat atacgacttt
     7981 cttaccaact tttcttagga gattgccagt tattgttaat ctacctagat ttcaacaacg
     8041 tccgtcgttt gaacgtctgt cattgattga tgaatttttt gtttctgaga gtaaaatttt
     8101 agctaaggaa ctatctgttt ctgatgcatt gattcatttc ttaatgaata gtgattttga
     8161 aggtaacgtg gggaatatta aaaatattat caagtatgct tgcggaaacg catatatcca
     8221 ccagaagagt cagcaaacaa ttcaagttaa attattggat atgcctttag agtacagtta
     8281 taaattcaag gagcaactaa ataaacccag aaagaaaaga gcagatcgaa tctactatcc
     8341 agaagaaaca cgacaagtgc aactagaaag aaacaatcag ctttttgttc aattttttga
     8401 ccaattactt actggattct ctgaagtgat tgaaaataac aaggatatta aactattttt
     8461 agaagaaatg gttaccaagg taacacaagt aatggatacg cttatttttc aagaggatta
     8521 cgaaaaagag cagtctttat acgccattct aaactatcat attcgtcaaa gtattgattt
     8581 tatgcgagaa agctacgggt ttgaacaaga tggtaaccgg gtagtcgctt tggccaacta
     8641 tctatatgtc aaggataatc aagtgatttt tgaaaatagt cttcaagaaa agaaaaccaa
     8701 agatcagctt aacgaatttt ttgctaatgt tcttgaaacc ccttattggt atgcaaaaaa
     8761 aatcttaagc cttttatctg agtgtttaga tcaacctgtg tatgaagagg acattttctt
     8821 tattacgcta tatttttata gtttgaatgt cacgaatgtt aaaacaggta ttaagagtat
     8881 tgtgctagct catggttatt ctacagctag tagtatggcg aatgttgcca accgtatgct
     8941 gaataagaac gtttttcaag cttatgatat gcctttagat attacattag agaaaattga
     9001 agcaaaattg attcaattcc ttggagatta tcgaacagat tcaggcttga ttttattggt
     9061 agatatgggt tcactaaacc aactaggcgg tttattagtg aatcatctaa atggaccact
     9121 gttaatcatt gatcatatca atacaccgtt attattggaa gtcggccaac aagtattaaa
     9181 caataaaagt atcaccgaaa taggagcaga aattgaaaca agtcttactt tcaaaaagca
     9241 gttactactt cccaagaaaa agaaaaagaa agccatcatt acttgttgtt atacagggat
     9301 tggtagtgcg acccagattc aggagatttt aacgaaatgc ttgaaagatg cagttagaga
     9361 attagtaatt attccgtatg attacaaaaa gctgttagaa aacaagaatt ttgagactcc
     9421 ttttcaatta tacgatgtca tcatgattgt aggtacagaa gatcccaaaa ttaatcagat
     9481 tccatatatt ggattagatc gcctgattaa tggggaagca attggcgaat ttgttcatta
     9541 cttggaaaaa gagctgacta ttgaagtgaa cgatttacaa aaagaattaa ttttcaattt
     9601 ttcaattagt aaaattgtcg aaaatttaac cattttggat ccagataagg tgctatcttt
     9661 tgtacaaaaa gcaattaaag aattatcgaa gttattaaat tttgattttt ctaataatca
     9721 gctgtttttg ttgtaccttc actgtagttg tatgattgaa cgaattgtac gtaaagaaag
     9781 tgtggatgag caagaggaca ttgaagaata tatgcaagca aatggagaaa taatggataa
     9841 aattcatagc gctttttttg agattgaaag ggaatacaca attgaaatac cagatttaga
     9901 gttgcgatta ttgagcgata ttgttcaagg ataggagtga attcgaatga caatgggagt
     9961 aatttttgat atggatgggg tactcataga cagcgagaca ttttattttg agagaagaat
    10021 gaacttcttt caagaactgg agatagatcc aggatcaaac cagattgatg attatgtggg
    10081 gaaaacagaa gctgcaatat gggaaacatt agttcctgag gacaaatcat taagagagga
    10141 actttatccg aaatatgtag attatcgaaa aacgcatcca attgatttcg ctaaagattt
    10201 acgaaaagaa gtgaaaccgt tacttgactt cctcaagaag cgggatatac cattggcact
    10261 ggcatcttct tctccagttg ttgagataga gagtatgctc attcaatgtg aacttactga
    10321 atatttttcc tttgttataa gtggtgagtc attaaaacaa agcaaaccac atcccgaaat
    10381 atatttaaaa gcgaaacagc aattagactg tgacttcttt attgcagtag aggattcaac
    10441 gattggtatt aaatcagcaa aggcagcaaa tatctatacg gtggcactaa aacaaccaat
    10501 gcaaatcgat cagagtgaag cagacataca aattgaaagt ctacaagaac tggaacaaat
    10561 tgttttagaa aaaggttagt gaattaatgg aaataaggct atatatctgt gctagctatt
    10621 ttgactaacc aaaaaaactt gctataattc aggtgtagta aaaggtgctg gtaacacctt
    10681 ttacgaaaat gcttcggcag tgataatggg attggctttt taagtttttt attttgctcg
    10741 caatccctaa ggtcatgtga atgatcctag ggattgctag aatattttgc aacattctat
    10801 ctatttcaga acgtaacctt ccccagttca ttgggattca ttagcataca accaacattt
    10861 caaaaagaaa ttgcactaca ccattattag ctattttgac taaccccaaa aaacttgcta
    10921 taattcaggt gtaggtaaaa ggtgctggta tcacctttta cgaaaatgct tcggcagtgg
    10981 taatgagtgt ggcttaaagg actagtcctt taagcttttt actttgttcg caatctctaa
    11041 gatcaagtta atgatcgata agattgctag aatattttgc aacattctat ttcagaacgt
    11101 aacctttccc attccactgt gattcattgg caaccctcct cacaaccaac gtttcaaaag
    11161 aaattgtaaa gttgttgggg taccacgacc gaacaatact acacctgaat gatagtaagt
    11221 tgtctcatta agtatagcat atcctctttt tgggtaaata gaaatcggtt acatttttta
    11281 tccaagacca agttttttca tttggtctta tttgctatac ccaaacaatt ccgcagtata
    11341 gtcgacggta ttgattttat caggtgagta atagaaactt gttttccttc cccaaaacat
    11401 tacctataaa ggagaaaaaa tggtatcgta attatatagg tataggatga aatttttttc
    11461 taagaggagg gttgttgatg aaaaatttac agttaaaatt tattgttgat ccatcgattg
    11521 tacgctggtt tcggattatc aatcaatttg agcgcaatca acttgccaca aaaggagaat
    11581 tagcaaagag taatcatgta tctgaacgaa ccattcaaac agatgtcaat aaaatgaagg
    11641 actacttttc tgaaagtgtt caatttgttt caacgaaaag tgggtattct tttcagaagg
    11701 aaagaccggt tctttttctc aagcagaagg agcaactact ggagaacgaa attttatttg
    11761 agttgttagg gaatgtcttt tatggcgaat tggaagatat gacggagcta attgatcgtt
    11821 ttcatttatc tgaagcgact tttcgtcggt atttgaagca gattcaacca gttttggaag
    11881 aatacggctt agaattgagt ctttatcctc ttgatttaaa aggagaagag gcaaatattc
    11941 gcaagttttt taaagacttc tattatgagg gggagatgac gccccatact cttgttccac
    12001 caagggatct tcatgaactt gtgcaagcta cattctatga taaatttgag tatttctcga
    12061 ttggaacagg gacttctcct gctgaatttt attatagttt gtatattgcg attgagcgtg
    12121 tgagacaagg aaaaacgata gccattcctc aaggtctgat tgagcgagta attgctagtg
    12181 agaattttcg cttaatgtat tccttgaaag aagcgattca aacaaattat caagttgctt
    12241 tatcagaaga ggagttttgt tggttgtatt
//
LOCUS       DACECY010000074        11954 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.079, whole genome shotgun sequence.
ACCESSION   DACECY010000074 DACECY010000000
VERSION     DACECY010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.079"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1153..1551)
                     /locus_tag="IWO38_002304"
     CDS             complement(1153..1551)
                     /locus_tag="IWO38_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314374.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKYT
                     SMKPCILDWQWLIDAVLDVKCSKYNITLGC"
     gene            1767..2822
                     /locus_tag="IWO38_002305"
     CDS             1767..2822
                     /locus_tag="IWO38_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HAQ0314375.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2842..3186
                     /locus_tag="IWO38_002306"
     CDS             2842..3186
                     /locus_tag="IWO38_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314376.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDSKVAEVKNKPTDHNPIQQEHPSTVTSGHSAKKEKPML
                     KGFNAPSNKDLL"
     gene            complement(3367..3663)
                     /locus_tag="IWO38_002307"
     CDS             complement(3367..3663)
                     /locus_tag="IWO38_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314377.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETRSSLVDK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3667..4485)
                     /locus_tag="IWO38_002308"
     CDS             complement(3667..4485)
                     /locus_tag="IWO38_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0314378.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4705..4926
                     /locus_tag="IWO38_002309"
     CDS             4705..4926
                     /locus_tag="IWO38_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAQ0314379.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
     gene            4928..5194
                     /locus_tag="IWO38_002310"
     CDS             4928..5194
                     /locus_tag="IWO38_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0314380.1"
                     /translation="MSYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            5307..5846
                     /locus_tag="IWO38_002311"
     CDS             5307..5846
                     /locus_tag="IWO38_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314381.1"
                     /translation="MYNKSVNKLINLQKERFFMGWYGAYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKRQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            6337..6621
                     /locus_tag="IWO38_002312"
     CDS             6337..6621
                     /locus_tag="IWO38_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314382.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            6628..6873
                     /locus_tag="IWO38_002313"
     CDS             6628..6873
                     /locus_tag="IWO38_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314383.1"
                     /translation="MWTLEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            6895..7149
                     /locus_tag="IWO38_002314"
     CDS             6895..7149
                     /locus_tag="IWO38_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314384.1"
                     /translation="MNYTDEEQITLEYVFTNEEGEEIEKVLIISTKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            7231..7416
                     /locus_tag="IWO38_002315"
     CDS             7231..7416
                     /locus_tag="IWO38_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAQ0314385.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            7483..7878
                     /locus_tag="IWO38_002316"
     CDS             7483..7878
                     /locus_tag="IWO38_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAQ0314386.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            7868..8245
                     /locus_tag="IWO38_002317"
     CDS             7868..8245
                     /locus_tag="IWO38_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314387.1"
                     /translation="MKSKKETEPMKSTHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            8246..8641
                     /locus_tag="IWO38_002318"
     CDS             8246..8641
                     /locus_tag="IWO38_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314388.1"
                     /translation="MKQEKRKMKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLW
                     KYKILFLFWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
     gene            complement(8947..9345)
                     /locus_tag="IWO38_002319"
     CDS             complement(8947..9345)
                     /locus_tag="IWO38_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAQ0314389.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(9537..10304)
                     /locus_tag="IWO38_002320"
     CDS             complement(9537..10304)
                     /locus_tag="IWO38_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0314390.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            complement(10339..11562)
                     /locus_tag="IWO38_002321"
     CDS             complement(10339..11562)
                     /locus_tag="IWO38_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAQ0314391.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLEDHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGRTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGTEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
BASE COUNT         4116 a         1995 c         1813 g         4030 t
ORIGIN      
        1 acttttgttt cgtgttaagt ttctttatat tactacgcgc cttgcgtata aaacttcttt
       61 attcatccca cgatccatgt tattttatat ttcctttttc atgttatttt tagccagaaa
      121 ccgatcaagt ttttacctat tcccaaaatc catttattcg ttattttttt ctgattaaga
      181 ataattattt gatgcttaaa ttagctaaaa aagcgatttg ttactgatat aatcatgata
      241 ttaaagctga attaggcaga aagataaaat gttcgcaaaa tcaaagtatc aattatcttg
      301 tttttttata aatttgtaag taaaaatcta aaaagttttc ttttaattgg tatatgtatt
      361 aaaatcacat tcaatcactc ataaaagtat gcgatttcat ttttttggat gagattaata
      421 tcaataaata acgatgtaaa aaccatttat tatgctcttt tataagggta ttaattgatc
      481 taatttgaaa aaataattca caatttaaat gttttaatat tacgaataaa ataatgttat
      541 cgtaaaatgg tataaaaaag ctaaatatta tatttttgtt attttattag cagaaaaatt
      601 taaatagttt tacaagaatc cgtagaaata tattggtttt gccttcaaat taatactgga
      661 tcatattaat ctgaatataa tatatgaact aataaatagc ttaacctagc tattttgttt
      721 tttctgatca ctatttatac agcattagta aataaaattt taaaaataaa aaaaataagg
      781 caaaaaaagt atcaacataa acatatacat atacacgttt atgttgatac acttaatttg
      841 tcctttaaaa agcacttttt tgatattatg ttattttata atcttattac aagctgattg
      901 ttaggcgttg ccctttcatt acatttatta acggttattt tatcgtttaa tttattaatt
      961 atttttgatc taaaacttag cttaacaaat aaataattaa ccaaccgcca gataaaaatg
     1021 cgatcattgc taatacataa gcaaagataa aaatatgttc gatcagctta aaccctgatg
     1081 cgattaagca gaccaagtaa aatagaaatt ctcttacaga taatggtaat tttctaacta
     1141 gcatactcat aattaacacc ccaaagtaat attgtatttt gaacatttta catccaatac
     1201 agcatcaatc aaccattgcc aatccaaaat acaaggcttc attgaagtat atttttcaga
     1261 tgaaaagata taatataatt tcttcggaat aatattttca gttgttggga tcaagctaaa
     1321 tctaataccg tctaactcat agctaccagc tttctctcca ttaaaaattt ccttagcact
     1381 ttcttctaaa gaatcaaaat atttttcgac agcaaccttt gaagctttta ctattgaagc
     1441 atttcccttt tcgataaaat ctgcttttgt tttaccgaca ataagtaatt cttgatcagt
     1501 aaaatcttct aaaataatgg tagtgtaatt cttatttctt ctcaatcgca tcaggatcac
     1561 tccttaatat aatttatgtt tttataatat cataataaga taaatttgca agcatttatt
     1621 tacacctttt tatttacact ttattataat taattatact tttatgattg catttttttc
     1681 ttataaacag ttgcaactaa tagtaaatag gtgtaaacta ttttcgtacc gaaataccat
     1741 aaacacaaac agaaagaggt cataccatga aagaaccatt attgttttcc gttgacttag
     1801 ggaataaatc agtaaaagaa atgagtacac acgtttctcc actctcactt ccctctcgta
     1861 ttttgaacaa aaatatcatt aattcaggac gtttttctaa tataggttcc tcattaaaac
     1921 gatcactaaa cgtttctgaa tatagcctag taaacactac tgaaagctat tactttggga
     1981 aggatataca cgagctagga aaagatgact acgtgataga atcactaggt tttgggatca
     2041 gcagatacaa gcaggaaacc taccaaaata tcctatcttt ctctttagct aacctagcac
     2101 gtttagaaga tcaatctgat gacgttttaa ccgttaatct agttttaggt ctgccttcat
     2161 ccgactatac cgaaacaatc gtcaaatacg tgatgcaaca agccacacga caacattcag
     2221 ttgaagttga cggtaaggct tataatataa acgtagatac cgtttgggta ctgccacagc
     2281 cgattggaac actatataac ctcctacttg ctgatgatgg tagtgtacaa gatgaaacat
     2341 tgatcagtca gaagatcggt tttgttgata ttggcggtgg cacgaaactt tttgatgttg
     2401 tccaaaattt tgaaattgac gaaaacaatc gtgaacaaaa ggataccggc atttttactt
     2461 tatataatcg tatcgtaaat atgcttgacc ttccgatcag tcctaatgct ttccaacttg
     2521 aaaagattat ccgagaaggc aaagaaaaag gcgtatatct atttaagcca agcggaaatg
     2581 aaagttttga tatttcagaa gtagttgaaa gagaaataaa aacatacacc aatatggttg
     2641 ttgcagaagt tttagctaca ttcaaaaact tcacaacctt agataaagtt gtctttactg
     2701 gtggtggagc ttctattatc gacaaacaac ttgtaaaaga taaaattccg cataccttgt
     2761 ttgttgaaga cagtgaattt tctaatgtaa aaggctttta caagtttggt ttaactcaat
     2821 aaaacagcga aggagatcaa catgaataag ggaaaatcag agcgttatgt agttataatc
     2881 aatagagaaa aggttagcga gttaccttta gcagagtttc ttgaatccga atatgaaaca
     2941 ctaggcgtta aatactctca gacgatcaaa aaagctttga acctatatcg gcgtgtaaag
     3001 aacaatgaag ttacaattat tgaagcagga aacgatagca aagtagcaga agtaaagaat
     3061 aaaccaactg atcataaccc tattcaacaa gagcatcctt caacggttac atccggtcat
     3121 tcagcaaaga aagaaaagcc aatgctaaaa gggttcaatg caccaagtaa caaagactta
     3181 ctttaaaaat aaaaaggcga ggttacaata cctagccttt ttattatgta caaatataaa
     3241 cataaacata tacgtataca tgtatataaa catgtatacg tatataacca taaacacgta
     3301 tatgaaattt agttaagcta tacctaaccg cttattccaa taaaaaaatg atcagcttta
     3361 aactgatcac tcaccaaaaa cttcatcata caagttatct atcatttcgc caagtaaagc
     3421 tgaatctgat cgaattccta acttttcgct gattttagat agtttctttc tttgcgatgg
     3481 ttgaagcgtg aattgataat ttttcttctt ttcagaacta tcacttttta gtttatctac
     3541 caaagaactt cttgtttctt taggtgtttg tttaggttct tcaactgctt caaccacttc
     3601 aggaacttct tctacttcag cttcagcgaa ttttttaaat ctattttctt tcacttcaat
     3661 cactccttat tcttgtgccg tatcaattat tttgttgtat agcgtaaacg tgctttcaaa
     3721 gaaatcctta ttcttacgat agatattctt atccttagcg aactcagcaa tattcttacc
     3781 ttctaagcta gttgcattaa gcaattcctt ctcttgtaca tatccaagat aattagataa
     3841 cgttggtacc aattctagga actcacgact tgaattagta ttatgtttga tcctgctacc
     3901 gatcgtagcg tatttagttt ccatcagtgt ttcaccagtc ttaatatcaa tcaactgttt
     3961 tgctagtgca tcaatatctt taccaagctt cagcaaagct tcaaaaccat ctttagatgg
     4021 atcagaaatg cctacgacaa gatcagatac agcccaagca ttttgagcta gtagacctgc
     4081 atcattatga gtatcaatca gaattagatc atacttctct ttaagctgtt catagttaac
     4141 aacgatccat ctaaacagta gagttctgtt gttaggcata tcacgcattt gcatctcaac
     4201 ttgttttaaa tcttcacctg ctactaaaat atccatattc tcatttatag ctaaaggttc
     4261 agctgatcca tgaagatata aatgattgat tgtattctct cttttcgctt tactgaaagc
     4321 atcaggaaag cgttttgata ggttttgcga cttatcttca tcaatataca gcaccttcat
     4381 acctaaattt tcgacagcat atccacctag attgtagatt gttgttgttt tacctgttcc
     4441 acctttttca atgtttccag taatgattga caacttcttt tccatgatta aacctccctt
     4501 attaaccttt tgtaactcca ttatacacta taaacacgtt tatgtatatg tttatataca
     4561 taaacgtgtt tatatttata tttgctttta tattctacaa cataaacatg tatatgtata
     4621 tgtttatgtg tatgttcata tatataacta ttgcatttgt catacaatcg tagtacaatg
     4681 taattgaaaa ggagttgata aactatgcca gtaataagta ttcgtgtatc taaagaagaa
     4741 aaagattttc ttgaaaaagt tgctgaattc aacggagata acatttctga atttatgcgt
     4801 agtaattccc tgcaaacagc agaaacgtta gtagattttg ccacctacaa ggaactaatg
     4861 aaagagcatg aggaaaacga tcaaagtatt tcccacgaag atatgctaaa agagttaggc
     4921 ttataagatg agttaccaag taagatatga acgtgaagct caaaaagcgc taaaaaaaat
     4981 ggataagttt caggcaaagg ttatcctaaa ctggatcgag aaaaatcttg taggcactga
     5041 tgatccaaga aagcatggta aaggtctaac agctaataaa tcaggctact ggcgatatag
     5101 ggtaggtgca taccgtataa tagctgatat tagcgagcat gaagtgacaa ttcttatatt
     5161 gtctattggt cacagaagtg aagtttataa gtaacagaca agctgataag gcttgtctgt
     5221 ttttttgtta acccaacaaa atcactgttt acgatcccac tttacctaaa aaagaacaat
     5281 atcctcttgt tattatacac attggtgtgt ataataaaag tgtaaacaag ctaattaact
     5341 tacaaaagga gcgatttttt atgggatggt atggtgctta ttcatcagca aatgaagtga
     5401 tcgaagaaaa gaaagggtac gccaaaaatg gtttggaaat cgtagatacc aaagtaacaa
     5461 agacctatgg aattatcctt ttcaaagatg cagaaggaac ctacgatatt gacagcttca
     5521 tctttcgaga tgaaatgtac aaaccaatct acttcttaaa tgacagtcct gcttacttta
     5581 agaaaattcc gaaaaaatgg ttgaaattct ttgtcggtga tcaagaaaaa atggttaagc
     5641 gacaactaga tagaattaaa gaagaaagca ataaaccaaa acttgatgac ttattagttg
     5701 caggtaaaac ttactcaatc tgggatggat cgtatgtcgc tgtttatcag tataaactca
     5761 aacgtagctt tatttttcga ctacaagatg gatcactcac tcggttcact cgactatcag
     5821 caggagatgt taaggaagta gcctaaaact taaatacata aacataaacg tgtatacgtt
     5881 tatgtttatg tatatgtata tgaatataaa cgtattaggt acgaacgctt ttaaatgctt
     5941 ttctgggctt tgattttagg gtagagctaa aacatcacca tatgatccaa aacagctcag
     6001 aaacgcattt aaacgtatct gataccaata ttatagaagg agtgtatcgt atgattaacc
     6061 tatttctttt ggtgacagct agtacattat tctttactgg aatatcactt ataatttcct
     6121 atttgatcta taagaaagcc gaaacttaca caagcaaagc aaccggacta gccgtttcat
     6181 cctttttaac tttgctgact ttttttgtta ctctatcgtt ttcttttgtt tagcacagct
     6241 gatccatcag caaaaaacct ctctttacat tatacacact agtgtgtata atgtaattgt
     6301 aaacaagcta attaactttt aggaaggagt gacaatatgc taacagctac attacagcaa
     6361 cctactcacg ttgctattgc agaaagctat acaattaatg cagggatcgt taaacagctg
     6421 atcaaggatc gagaattcaa aaagtttaac agtcgtcaag cgtataacag acagttaaag
     6481 aaagctattc taaagcacaa aaattccttc atcgggaaat tggaaagtgt ggatgttaaa
     6541 actgtttcaa cggcattttc agttgttgaa gtgactattg acggtcgctt gatttctcta
     6601 tttgcttttg gaattaattg atagggagtg tggactttgg aaggtaagac ttttgcagaa
     6661 atttggactg aatttattga ggtaccaact cgcattgatc cgataacttc tgaaaaggta
     6721 ttggatgcag acttctacaa tctcaaagca ggtactagcc ttgaatgcgt ttggatgtat
     6781 ttagaagagc atttcaaagg cgtaacgctc ggtcacgtct tataccacaa tggtgccgag
     6841 tgggatgcaa ttggaaatat ccatttttct taatacaaga aaggagaaca caatatgaat
     6901 tataccgatg aagaacaaat cactttggaa tacgttttca caaatgaaga aggggaggaa
     6961 attgaaaaag tcttgatcat ttcaacaaaa gacttggaga aggctttggc ttatgaacca
     7021 tacgaatcaa ccgtaactgt cattgaattc ttagatacct atacttctaa tgatacttgg
     7081 gaaattatga atatttgcaa cgctaataac attcaatttg ctgaaaaaac caataaatca
     7141 ttttattagt tttgcaataa aacatcctct ttttctattg attacataca caacaatgtg
     7201 tataatagat tttgtaaagg gagggtagct atgcctaaga cagcaagaga aattgtaaaa
     7261 attctcaaat ctaacggctt caaaaagact ggacaaactg gcagtcatgc aaaattttat
     7321 aattccaaga ctaaaagaac ggtcgttgtt ccgatccaca atggtgatgt accactagga
     7381 acagaagcta atatctggaa gcaagcaggg ctttaacagc cttgccttcc tatttggtat
     7441 taggcatact atcctctctt acactagaaa aggagcgagg aaatgaagac aaaaaatatt
     7501 ttttatccgg caatcttcga gaaagaagga gaagcttata acatcgtttt ccctgactta
     7561 ccggaaatat ctacctttgg aaatagtctt gaagaagcat atttaaacgc taaggatgct
     7621 ttaggattag cgctatatag tgtaccagat gcagaatttc ctaaaccatc agcagtggaa
     7681 gaattaactt tgaaagccaa ccaagtggta gtaatcatcc aactaaacat taaacttttc
     7741 cgaagaagct taaatacaaa aacaattaga aaaaatgtta gtgttccgga atggttagtt
     7801 cttttaggga aggagaaaaa cataaacttc tctcaactac ttcaaaaagc actagaagaa
     7861 gagttgcttg aaaagtaaaa aggagaccga acctatgaaa tctacacact taaactattt
     7921 ctctcgaacg ttaatcaata atctattaaa agatacaact ggcattagcg ttagtaccaa
     7981 acactttgcc aatgatctaa ttatttttga ggataataac ttgcgaattg agatgtttat
     8041 tgaacaggca acccctaact caatacctga attctgctat acgcacactg aaaaaatgaa
     8101 acatgcaaaa tcagttaaag atgttgaagc attaagtgaa tcactaacga acgctttaaa
     8161 cctcgttaag aaggcaaaat tgttatttga atttcaaacc gactaccaca atgagttgct
     8221 tgctcaatct gaaattttag attaattgaa acaggagaag agaaagatga aacaaacaca
     8281 aacagaaacc gttaacgata cgcttgctga tcaattagca ggttattata aattatttga
     8341 gatgggtaaa gcacagcgct tgatccacta tctttggaaa tacaaaattt tgtttctttt
     8401 ttgggaatca acactaacat tctcagttct tttttataag tcagattttt ataatcaaca
     8461 cttagtttta gggagttttt tgcttatcta caccattttg atgcttaatt tatataccta
     8521 catcacactt gaagaacact tagtaattag cgatgcaggg atcgagagaa acatcaaaag
     8581 ggtatctttc aagatcacgt taaacaagcc taccgataaa aagctctcag aagcgttctg
     8641 agagcttttg acattttctt gtataattta tcctaacaca ttataaagca cctaattcga
     8701 gcttatatca agcaaatttg ctatttatta cttaatacat atctaaaaat tacttgatgg
     8761 aaaactagat acatggtaag catgaaaaga aaatcaattt gagcagatgc agtacaatac
     8821 atagcaattt aaaagctctc agaaggcttc tgagagcttt taatatattc tttgataatt
     8881 tgtctttctt aaatccatac gtcctaaaac aagctgtttt tgcttaatat gatcatctga
     8941 gagacatcaa atcaactttt ctctcaacaa atcattcaaa gctttcgaca gattaatatt
     9001 ctcttcagcc gctaaaacat ttaaatagtg ttccacagtc actgattttc gatcataatt
     9061 ttgcttgttt cgagctttag caactttcgt attagctttt acgacaaata aattaacatt
     9121 ctcagatcgc ttgattcttg catttaactc ttgaacaggc gtaggcgatg gaatttcttt
     9181 ttcttgcaac tctaaaagca ccaagtatcc ttccaatact tcttgcgcca tctcaaaagc
     9241 atcttctaaa gtttcaccat gtgtatataa gtctggaaaa tctgggaatt taactgcata
     9301 ttcatttcct ttccattcga atactgcaca aaaaaagtat ttcatcgcat tgcacctctc
     9361 taattaaaaa ataatttttg cttttttgtt tcaaaaaaaa gaatcgccta ccaaaaaaat
     9421 tgataggcga tcacaagcta attaacctct aaaggagatt aataccttgc tcccaaggca
     9481 ctatatatct tagcatacgt ctgaagcatc ttcaaacgag taagatatat tttgcattaa
     9541 cctaattttt tttctctata agttttttca tcaaactcac tagaaatatt aaactggaca
     9601 gcaaaattgt aatttctgcc ttttctaact ttagcaaaat caatattcag cattgatttt
     9661 tcattaattt cctcaactga ttcccttaaa acacgtctat caaaattcga tattgtttta
     9721 tactcatttt ctgttttcgt aatctcacga attttaacta tttcaatcag gggattctta
     9781 tatatttcag ctttctcaaa atctccttta tttttggcga taagatattt tttataatac
     9841 atcgccaaat atttgtataa cacgatgcta tatttttttt gaaaagatgc taagtcttca
     9901 aaaacaaaga acggatactt ctttttgatt gagacaatta agggaattac atcttctgaa
     9961 aaactgaaag atacactatg ctcttctaaa tcccaatgaa tccgatcaat agcagataca
    10021 gtagtatacg tcttatattt tttgtctaga ttcgaaattt cataaactgc ttgtctttgc
    10081 aaagtgttaa ctatggtttt tagttccgtt tcaagatttt gatcaacatc catgtaatta
    10141 ataatcgctt taacaggtaa atctactacg cctgttttct ttagctttgt gggtgagata
    10201 taactgaaca gcaattgata aagttcatac gactttaaat ccattttcat gtcaattgtt
    10261 gctaagaact cctcattgta ctttgtcagt tcatcttttt tcaaaagttt ttccttcctt
    10321 cacgcatttt ttcagttgtt aatcataaag gttcacataa ggcaaataac tcgtaattga
    10381 tttttcgaga tatttaacag tattttttat tgtgtaatcc tccttatgag tagcaacata
    10441 agataggtga ttgacaactg attcaaatcc tctatctttg gataaacgat cataaagcgg
    10501 ataaacttta ttctgaagtc ggctcataag tttaagatcg cataagtcgg cagaactgat
    10561 caaaaagttt ttgattagta atttagtgta tttgctattc atagctttca agtacgattg
    10621 attcattttc tctgttttat cttctgtgcc ttttaaataa gcagggtctc tttgttcttg
    10681 cttataaggt accggaactg acatggtacg tgtaagcaag aactttatat ttgtataagt
    10741 acgacccttc ttgataagct catagtcaat tttaaaatca gtgtatttat tcagttgggc
    10801 aatcggttct ttaattacac gtctttcaaa gtctcgaaaa gctttatatt ttttctcaat
    10861 actgaatttc tttcgtaaat ctgcaatgga gaaaacagga ttttttaatc tatcaagtga
    10921 ttttgtactc catgttaagt tatctctatt tgcttcatac agattaaaat ctttagtgaa
    10981 taattgaaaa aaactgattg catacttgct tgtcaagtta actacttgat caaatgtata
    11041 ttgcgtaaaa tgatcctcta agttcgttaa taaaggcata attgaactac taaatttaat
    11101 agatactgca tcagaaaaat cattgaattc agtaatttct agcggtgaga tgactttgta
    11161 ttcagtctta ccgttaggtt tagttctttg aattttaatg acagattgct gatgcaattt
    11221 ttcaataact tgttttaatc ttgaatgttt tgaagaagat gaactagaag acatcaacgc
    11281 aaataaagcc tttttagata ctttgacggt attattaaaa ggcggatttt tagggtcaag
    11341 atgtcctata gcgtaaagat aaattttttg tggcatagca tccatttcaa caatgctatc
    11401 tacaagttga ttcgataaag taacagttaa atcaaacttt tttagattgc tttcggtagt
    11461 ttcaaatttc gggtttacat tcttttctgt tattttcata cgattgcctc ttctttctaa
    11521 gcttggtacg agcttaaaaa cttggaactg gtacgattcc attgaataag tatagacctg
    11581 ttttcgatac gttacaataa gaaatgtatc gtaaatttat aacataactc aatgtcagta
    11641 aaacacattt tattttttcc aatcaaattt cattgaaaat acagcagaaa tcgctatttt
    11701 agaacatttt caaaataaaa atccttttca aaaaaatacg aaattataaa aattaccaaa
    11761 attaaaaaac aaatattttt ctagttttta gaaataaatt cacatacttt ttcttttttt
    11821 ggatatattc ggaataccac atcgcttttt gtccacattc acatcgcttt atgtccaccc
    11881 taaaatctat ttaagattga tataaagcct ttttggaggt tgctaaagtt ttaaagatta
    11941 aaatatatat tata
//
LOCUS       DACECY010000075        11767 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.080, whole genome shotgun sequence.
ACCESSION   DACECY010000075 DACECY010000000
VERSION     DACECY010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.080"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            274..3501
                     /gene="fss3"
                     /locus_tag="IWO38_002322"
     CDS             274..3501
                     /gene="fss3"
                     /locus_tag="IWO38_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ0314392.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            3613..3927
                     /locus_tag="IWO38_002323"
     CDS             3613..3927
                     /locus_tag="IWO38_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0314393.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3940..4314
                     /locus_tag="IWO38_002324"
     CDS             3940..4314
                     /locus_tag="IWO38_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0314394.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            4324..4668
                     /locus_tag="IWO38_002325"
     CDS             4324..4668
                     /locus_tag="IWO38_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ0314395.1"
                     /translation="MEINVEHILDCLDQFGKGELTGEQLTNALTYDEKMFLIMHQGLL
                     EVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFLKQL
                     VETKQLPVVVND"
     gene            4737..5753
                     /locus_tag="IWO38_002326"
     CDS             4737..5753
                     /locus_tag="IWO38_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0314396.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            6347..8221
                     /gene="ltrA"
                     /locus_tag="IWO38_002327"
     CDS             6347..8221
                     /gene="ltrA"
                     /locus_tag="IWO38_002327"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0314397.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            8347..8703
                     /locus_tag="IWO38_002328"
     CDS             8347..8703
                     /locus_tag="IWO38_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ0314398.1"
                     /translation="MILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRV
                     ALGRMSELGYSMMFGEVDKNFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREIS
                     NIVGLTVHTERENNSV"
     gene            8748..9890
                     /gene="dcm"
                     /locus_tag="IWO38_002329"
     CDS             8748..9890
                     /gene="dcm"
                     /locus_tag="IWO38_002329"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ0314399.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            9880..10169
                     /locus_tag="IWO38_002330"
                     /pseudo
     CDS             9880..10169
                     /locus_tag="IWO38_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10425..11609
                     /locus_tag="IWO38_002331"
     CDS             10425..11609
                     /locus_tag="IWO38_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0314400.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            11624..11719
                     /locus_tag="IWO38_002332"
     CDS             11624..11719
                     /locus_tag="IWO38_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ0314401.1"
                     /translation="MEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
BASE COUNT         3942 a         2002 c         2601 g         3222 t
ORIGIN      
        1 ttcgtatcat tgaataagtt tgaaaagcct tgatttataa aggtttttcc gtaaatatat
       61 tttgttatat ttgattaaat ttgaaatgtt tgtgttctaa aatgtgttct aatttaaagg
      121 cagtatattt cgtattttat attaaatttt taggctcatt cattctcaaa tccattctat
      181 ttagtcaata aatattttaa aaagacttta taaacaaaga tgttcaagcg agcatcttat
      241 ttttttgtgc ctaatttgaa aggagaacat ttcatgtata aaaaacaaaa aaagaaatta
      301 tgtgtattaa ctattttgag tttggctgtt caatcaattg taggagcaat tgctcctacg
      361 attgcttttg ctgatgaaat tacccatcca caaacggtaa ccgtagaact agatttagca
      421 catcaatatg ctgttgaagg aacttttagt gatggtcgtc caatgtcaga agtcactgtt
      481 ccgcattatg cagtgtacaa cggcgttaaa caagatattt tctgtgttga accaggagtc
      541 ccgatttaca atgagttcac tcctggatat gagaagaacc cgttaccaga tatgtccgat
      601 aaagccaaat tagtttccgt tttatggaaa aaagtaggta cagatgcaga tactcatatt
      661 gtggcacaga agatgatctg gcaagaagtt aatgggtata cccttcactc aataaaacga
      721 tcagatggta gtgccgtaaa tatcgcagcc attgaggcaa aaatcaatca agccattgcc
      781 gattatcaga agaaaccaag cttccataat agtacggcta aaacagtatt agggcaatcg
      841 actactgtga cggatacgaa taatctgaat ttgtcagagt tcgatgaggt tgtggaaaat
      901 accgcaaaca ttgattatcg tgtgaatggc aatcagttga taatcagccc aaatgccaat
      961 tcaaaagaaa gtggtgtgtt gacgcttaag aaatctgctg gtaccggaac acctgtcgcc
     1021 tataaaaagg ctggccaaca gactttaatg gccggggcga tcgataagcc gaacacttat
     1081 acggtcaaaa ttgatgtgga aatggagggt tctttgaaga ttaaaaaagt tgataaagaa
     1141 tcgggtgata ttataccagg aacggttttc catttagact ttggaaaaac tttacccgca
     1201 aaagacgtga ccaccgataa agaaggcatt gcgaccttag atgagattcc tcatggtaca
     1261 aaggtaacca ttactgaaaa atcggtgccg gcgccttata cgattgatac cacacccatg
     1321 actgccacaa ttaaagcagg tgaaaccatt tcagtgactt caaaaaatac gcgagaaaaa
     1381 ggtcaaatta ttctggataa aaccggaatt gaaacaggga gtgatctttg gaacgataat
     1441 tactctttag cgggcaatac gtttgccatt cgcaaggata gcccaactgg tgaaatcgtc
     1501 caagaaatga ccacggatga aaaaggtcat gcagaaacac caaaagagat tgccaatgcg
     1561 ttggaattgg gaacctacta tgtgactgaa accaaggcta gtcatggctt tgtgaatact
     1621 ttcaaaccag taaaagtcga attgaattat gtcaatcaaa cagtggctct tgttaccagt
     1681 aacgtaaaag ggcaaaacca agaaattact ggtgaaacca ctttgacaaa agaagacaaa
     1741 gataccggtc atgaaactca agggaaagct gagtttaaaa gagctgaata caccttgttt
     1801 actgttaagg atggaaaagc ggtcaaatgg accgagaact ttaaaccaga attgttgaag
     1861 ggaacgaaag cttctgatga aaccgtaact ttgactttag atgaaaagaa tcaagttgcc
     1921 gttaaacact tagcgattaa tgagtattac tggcaagaaa ccaaagcgcc tgaaggatat
     1981 accttggatg aaacaaagta tcctgtatcc atcaaaaagg tggatgatag cgaaaaaaat
     2041 gctgtgatta ctcgagatgt tgcggcaaaa gaacaagtga ttcgctttgg ctttgatttc
     2101 tttaaatttg ctggatcaac tactggcact gccgaaactg gcttcaatga tctaaccttt
     2161 aaagtgtcgc cattggaagg aaccaatgaa atcaccggtg ccgaagatga agcgaccaca
     2221 gcttataacg agcaattagg gtttgatggt tatggcaagt ttgaaaatct tccttatggg
     2281 gattatttac ttgaagaagt agaagcccca gaaggttttc aaaagattac gccattagaa
     2341 atccgttcta cttttaaaga aaataaagag gattatacca agagtgatta tatctttact
     2401 attaccgaaa aagggcaaaa gcaaccgatt aaaacggtaa cggtccctta tgggaatcta
     2461 accaatgaga atttctcagt cagtttgaac cgtttgatgc tttatgattt gcctgaggaa
     2521 gaagatagct taacttctct tgcgacttgg aaagaaggag ataaagaatt gactactctt
     2581 gattctaccg agctagtcga taaattgagc tataatctac atgaaatcaa agaagactgg
     2641 tatatcgtag ctcaagccat cgatgtagat gctacaaaag ctgcccaaga aaaagataaa
     2701 aaagccaaac cggtggcgat tgcggaaaca accgcaactt tagcgaataa agagaaaaca
     2761 ggaacttggg agattctgca taaattaact gtcgaacaag ttttagacaa gaccattgtc
     2821 ttattcaact atgtgtatga aagcaaagaa gcctatgaag ctggtgatga gccggtagcg
     2881 aaggatgcta gcttgaacaa tcaagcgcaa actgttaatt gtatggtgga acgtcatgtt
     2941 tccatccaaa ccaaagcgca cttagaagaa ggttctcaga cctttactca tggtgacgtg
     3001 gtagatatgt ttgatgatgt atccattacc catgatgtac tagatggctc aaaagaagct
     3061 tttgaaacaa ttctgtacgc tttatttcca gatggtacaa gtaaagaaat ttggaaatct
     3121 ggcaagattg attacgaagt gaatgacaaa gaatttacca aaaccgtact tgcggaaaaa
     3181 gtggatacaa gtaaatatcc agaaggtaca agttttacct tcaaagaaat taactatgac
     3241 aaagatggga atatcaatgg aaaacacaat gaagatttga aagaaaaatc tcaaacttta
     3301 acgcccaaaa aagttccgac aacaccaagt acaccagaac aatctgaaat gccaactgtt
     3361 ccaagtggct ctcaagaatc tagtcctaca gtgaaggcat tccctcaaac cggggagaaa
     3421 acttccaatg tgctactgtt cattggcttt agcttgatct ttgcgacggc tggctattat
     3481 ttttggaatc gtcggaacta aggtgataag atgaaacatg taaatagtag aaaacaatac
     3541 gcgcgaccgc ctcctaaaaa agtggtcgcg ctatttttgt attcaaaact aacaatgaaa
     3601 agaggaaaaa atatggaatt gaaatttgtt gtaccagata tggtcgagac gttcggaaaa
     3661 ctaacatacg gtggtgaagg tgaagttcta tcggaagggt atggtcgaaa tactacaatt
     3721 gttggtcgaa gttaccatct gttttcaaat aaacaacggg ccgatgatat tgaagtggtg
     3781 gtagctgcgg aagccggcga aaaggatttt gaccaagatc aaccactaaa agctgtaaat
     3841 ccccacttaa ttgccaaagg ctatgaaatt gagaatcgtg ggtttaccga ttacgtgttg
     3901 tatgttgatg atttagtgaa agcataggag gaaaagaaga tgagattaac agaaggtatt
     3961 gtcgtagatt caggattaac ctttggcaag ttgcgttttt ccgcattacg tcgagaagta
     4021 cgcaagcaaa atgaggacgg aacggttagt aacgaggtaa aagaacggac ctataatttg
     4081 aaatcctccg cgcaaggtcg aatgatccaa gtcagtattc cagcaaatgt acccttacgc
     4141 gagtttgctt atgatgcaga ggtagagctg gtcaatccaa tcgtggatac ggtcgccaat
     4201 tatgtttttc gggaaggaac caccgttaat tggtttatca aggcagatga tttagtcttg
     4261 aaacgacaac tgaatcaagg aaaacctacg aaccaaaatg aaggaaagaa ataggtgaag
     4321 aatatggaaa ttaatgttga acatatttta gattgtctcg atcaatttgg caaaggtgag
     4381 ctaacggggg aacaactcac taacgcatta acctatgatg aaaaaatgtt tctgattatg
     4441 catcaagggt tactggaagt aggtaacgat gccaaagaag actttgatgt actggattgg
     4501 ttaggggaag aagagtcttt ctttatggtt gtcgaggtaa atgaaagttt atgtcggcag
     4561 gcagaatctg ttttggaaga aatcgatgtg gagatgccgg atgccatcga aagattttta
     4621 aaacaattag tggaaacgaa acaacttccc gtagtagtaa atgactagca taactttttg
     4681 aaattgccca atgaaatcct attgggcttt ttcgactgga ggtggaaacc atcaaaatgt
     4741 acaaaggaca tcgcatacgc gcaggagatc aacacttgat ttatcacttt gttcttggct
     4801 ggctaatctt tttgttcatt ggttggatga gcgtctttta tttacaagaa cttagacaat
     4861 ttgatatttc taaggtatca ttttccacta gtgaaattgg ctggtctatg aaggatgtaa
     4921 tctgcttgct aggaagtctc gctctttcag gaagcatgat gttactttat atacactttt
     4981 ttcaggatca ttggcgaagt ctttggcatc gacaaaagtt agctcggatg attctagaaa
     5041 accattggta tgaagtaaag cagactcaag gtgaaggttt tttcaaagat ttgaatagta
     5101 gttggaccaa agagagtatc agttacttcc ccaaaattta ttatcgaatg aaagatggct
     5161 tactttctat tcgcgttcaa atttcactgg gaaaatatca ggatcagcta ttgaagttgg
     5221 aaaataagtt agaaagcgga ctgtattgtg agttagtaga aaaagaactc aaagactcct
     5281 atgtggaata caccttgtta tacgatatga ttgccaatcg aattgggata gaagaaatag
     5341 tggcagaaaa tggggcgttg cgattgatga aaaatcaagt atgggcgtat gattccttgc
     5401 cgcatatgtt aattgctggc ggcacaggtg gtgggaagac ctatttctta ctcaccatta
     5461 ttcaagcact attgaagtca gatgcagaat tgtttatcct cgatcccaaa aatgcggacc
     5521 tcgccgattt aggtacgata atgcctcacg tatactccca aaaagaagaa atttctgaat
     5581 gtgtggaaga tttttacgaa cgcatgatag ctcgtagtag gtcaatgaaa gaaatgccca
     5641 actacaaaac aggagagaat tatgcgtatc tcggacttcc gcccaacttt ttaatctttg
     5701 atgaatacgt ggcttatatg ggtgcgaatc gttttcctac aagcatcgaa tagatgtgaa
     5761 atggaagcct attgagatta tcaatagtga caactaatgt tttgagagat ggaatgaaag
     5821 ccttcaagtt gagctgaaac atccccgcta atactcctac atgctctaat caatgaagta
     5881 gattggtttt ggtatgaagc taggtgaagt cgtctgaaat ctgcctaagt gcttgagcat
     5941 aaggctgact atagggcggg cggctgaaaa agataactat ggtaagaatg tctgaaaatg
     6001 actgacgaat ctgcgaatgt acgggtctaa aaggctgatc ttattgaaag ataggaacat
     6061 acggacgtat ggtgagatac cgacaagtaa ggttccatga tatgaaaatc gggatagcta
     6121 acgaagaagc tataatttca caggctcata gtagatacct aaggttatac atgatagcta
     6181 aaaacattgg aacgatgtaa gcagaaaact atcaattaaa tgactacttc tagataggtg
     6241 catataagta ataacgaaat tgctttgttt ctgtgaaagt aagggcacag taccgatgaa
     6301 gcgtctaaca aatgtggagg gatagcccta agtctattta ataacaatga atgttgaaac
     6361 agatgaagtt caaggatctg gtaagaacgt gggaaaatcc gttatggagg tgccacgctt
     6421 gaacacaaaa atacgttact gggaatatta tgatctacaa gaaacattcg atcagctatt
     6481 tactcaaagt agaaacggga aaaagtttta tcaattatat gagttaatca tttctgaaaa
     6541 taatattctt ttagcatacc gtactatcaa ggcaaacaag gggtcttcga caccaggaac
     6601 ggattccttc acaattgata actataagga aatgaaccaa gcagagttta tccatcttat
     6661 tctcagtcat ttagaaaatt acaaaccaaa atccataaag cgagtaatga tacctaaacc
     6721 caatggtgag aaacgcccat taggaattcc atgcatgatt gatcgaatga tccaacagat
     6781 gttcaagcag atactggagc caatatgtga agctaagttt tatgaacata gctacggttt
     6841 cagaccattg agaagtgcaa agcatgctct aggtcgcatt atgtatctca taaacatcag
     6901 taaaatgcac tatgcagtag acattgatat caaaggtttc tttgataatg taaaccaccg
     6961 tttactgata aaacaattat ggaatatagg aatttgtgac aaacaagtct tagctattct
     7021 ttcaaaatct ctgaaatcac ccattcaagg agaaggtatc ccgagcaaag gaacgatcca
     7081 aggaggtatc atttctccac tattatctaa tgttgttcta aacgacttag accattgggt
     7141 ctcaaagcag tggcatacct ttgaaacaaa gtatccctat actaaaggtt acaataaatt
     7201 tcgtgctcta agagatacaa atctaaagca aggctatatt gtcaggtacg cagatgactt
     7261 taaaatcatg actaatgatt atccgacagc attaaaatgg ttccacgctg tcaagcttta
     7321 cctaaaagac agacttaaat tagatatttc caatgagaag tctaaaatcg tcaatttgcg
     7381 taaatgtaag tcggaatttt taggattcgc tatttgcgtg aaacaaaaag ggaaaaaatg
     7441 ggtttgtaat tcacgtattt ctaacaaaaa aaaggatcag attaaagaag agattaaaca
     7501 aagaatcaaa gatattcaaa agagtcctac agcacaaaat gctctcctgt ttaatgctct
     7561 cgtgctcggt gttcataatt atttcaggta tgccactcat attaatttag atttgaatag
     7621 gatgaactac gttctttcat tcactactta caaccgatta tcaaattgta gtacaagatg
     7681 ttaccccaac aaagcttccc caacttacaa aaagttttac tcaacaacga tgaaaacgta
     7741 taaagtagct ggagtctatt tgtaccctct ttgcgacgta tctacaaaaa caatttacgg
     7801 atttaatacg gaggatacac cgtttactcc gtttggtcgt caaagattgg agcacaaaag
     7861 cctacagtct cttgtgttcc aagaattaag aaaattgatg gaatcgaaaa taccgaatag
     7921 aacggttgag tatttagata gtagaatatc tcgttactca atgaaatcag gcaagtgtga
     7981 gattacaaaa caatttttgc cagcaaaagc cgttcactgt catcactatt tgccaaaaag
     8041 tcttggtgga gacgacaaat tcgataactt gagaattatt cataaggata tccatcttct
     8101 gatccataca acaaataaaa tgataatcga tcactacgtg catgaactga aactggtgcc
     8161 agagcaaata gcaaaaatca acctttatcg aaagatgtgt aatctgcaaa acattcaata
     8221 gaaaataaat agatggaacg ccgtatgagg tgaaagtctc acgtacggtg tggagcgggg
     8281 gaaaagatgg agattatatc aaattcttac ctatcgctat agatgttaac gacaaaagaa
     8341 agtgcggtga ttttgaataa gcttaaacaa atcgtgatgt taggccgtca atctggtttc
     8401 tttctgattc tagcttgtca aaggccggat gcgaaatatc taggagatgg gattcgcgat
     8461 cagtttaact tccgtgtggc tttgggtcgt atgagtgagc ttgggtattc catgatgttt
     8521 ggagaagtcg ataaaaattt ttttatgaaa cgcatcaaag gacgaggtta tgtagataca
     8581 gggggcagtg tgattagtga gttttatacg ccacttgtac caaaagggta tgatttctta
     8641 agggaaatta gcaacattgt tggattaacc gttcatacgg aaagagaaaa taatagtgtg
     8701 tagcctagga atatcaataa tcctgggctt ttttattgag gtgatttatg acatttttag
     8761 atttattcgc cgggattggt ggctttcgct taggtatgga acaagccggt caccagtgta
     8821 tcggtttttg tgaaattgat gaatttgctc ggaggagtta taaagcaatc catgatacaa
     8881 ggaaggaggt ggaaatgcat gacatcacaa gtgtatcaga cgaatttgtt cgatccctcg
     8941 gaccagtgga catcctttgc ggcggctttc cgtgccaagc tttttcaatt gcgggaaagc
     9001 ggcaaggatt ttccgatact cgaggtactt tattctttga aatcgctcgg ttcgccactc
     9061 ttctccaacc taagtttcta ttccttgaga acgtccgggg attgctcaat cacgaaggag
     9121 gggctacgtt cgagacgatc ctccgaacgt tggatggatt ggggtatgat gtggaatggc
     9181 aagtgcttaa ctcaaaggcc tacgttcccc aaagccgtga gcggatcttc cttatcggac
     9241 attctcgaga tgcatgtacc gaacaagtat ttcctatcat tggatcgtct tcaacatctg
     9301 atcaaaacat cagaaacttg gtaaatatca atccttccaa tcgaggaatg ggtggccaag
     9361 tctatggttc agatagcgta gcaccaacct tgaccggtga tgaaggaatt aaaattgcct
     9421 taccggtaaa ggatgggatt tctgtagcgg gtatgttgtc gggaaatttt gaacaaggca
     9481 ataggattta tgaggtaacc ggaacagccc ccactttgtc aacgaaacaa gggggaacga
     9541 agattatgat tcggaaaaat gaaacagaat atcaagaggt gaagccaggg gacagtgtga
     9601 acctggcttt tcctaattcc acaagtcgcc gaggtcgtct gggaaaacaa agtgtgcata
     9661 ccttactaac aggagaccaa caagcggtag tcacggatca gtaccagatt cgaaagttaa
     9721 cacctaggga gtgctggcgg cttcagggct ttccagattg ggcttttgat cgagcttctc
     9781 aagtcaattc agatagtcag ttgtataaac aggccggaaa ctcagtaaca gttccggtca
     9841 tttttgatat tgctagaaga ttaaaggggg taaaagcata tgatttatga cgagttgatt
     9901 ggagaaatct actgggtgat tggcaagatt caggcagatc cgaaattgga agaagaactg
     9961 taccggctca attttgatat tcgcaaaaac ggggtgaaag tccaggtgat tcgtatgtag
    10021 tggatgagga aacggatgct cgaatggagc tcaatcaagt catcggtgaa ttggaacgaa
    10081 ttgcggacct tgccaaagaa cctgatatcc gccaatactt gtttgagata aaagcagagt
    10141 tggaaattga gggtatcacc gcggagtaat agggaaataa cccactgata ggcactgacc
    10201 gcgggggatt tgcgaagcag aaatcctccg tggtgctgtg cctgttcatg gacggcggca
    10261 gccggccatg atgtctcacc ccccgtatct aacagggggg tacaaaaaca caaaaaacaa
    10321 gcaagtaacc ataaaaaatg cttgttgtat caaggatatt aagtaatttg tataggtgtt
    10381 aactcatttg gaaaagttaa caccttgaat ttggaggtga aaacttggcg caacgaaatt
    10441 tagattatcg gttattaaaa gaacgacgca acgaatatgg cgtttcacaa aataaattag
    10501 ctactgcttg cggacttagc cgcccatacc taaatcagat agaaaacggt ggcgtaatcg
    10561 cctctacaaa gaccatgaga aaaatttttg atcaactgga aagtttcaat cccgatttac
    10621 ctttaacgtt actatttgac tatgtaagga ttcgctttcc cacaacggat gtgcggaaaa
    10681 ttattcaaga aattctccat ttgaaattcg agtatatgct tcatgaagat tacgcctttt
    10741 actcctatca agaacaatat gtcatgggcg atattgtagt gatgttatct catgaagaag
    10801 ataaaggtgt ccttttggaa ttaaaaggtc gtggttgccg gcagtttgaa acttttttac
    10861 tcgcccaaaa gcgtagctgg tacgactttt ttgaagattg tctaaaagcc ggtggcgtga
    10921 tgaaacgctt ggatttagca atcaatgatc gagtggggct actggatatt cctgatttaa
    10981 cgaaaaaatg tcagaaggaa gaatgtatct ccttgtttcg taccttcaaa agttaccgtt
    11041 ccggggaact gttgaaagct gatgaaaagg atggtatggg caatacctta tatattggca
    11101 gcctcaaaag cgaggtttat ttttgtttgt acgaaaaaga ttatgaacaa tatatcaagt
    11161 tagggattcc attggaccag accgaaacga aaaatcgttt tgagattcgg ttgaaaaatg
    11221 accgtgctta ttatgcgatt caagatttgt taaaaggtcg tagcattgaa agtaccacgt
    11281 tttccattat caatcggtat ttgcgatttg cggataaagt agatggaaaa cggcgaacca
    11341 attggccctt gaatgaacaa tggggtcgtt ttattggacg gaatcggaaa gagattcaat
    11401 taacttctga accgaaaccg tataccattg aacgaacatt aaattggcta ggtagacaag
    11461 tcgcccctac gtggaaaatg gctaaggagt tggatcgatt gaatcaaaca acgtatatcc
    11521 aggatatggt acagaatgcg cgactctcgg accggcataa aaagattttg gagcaacaaa
    11581 gtatggcaat cgaaaacttg attgtatgaa aagaggaatt ttgatggaaa ttgtaaaaga
    11641 tatgttattg gtacttggtg gcagtatgct tggtgttacc gtgatgtgct tgatgaatgc
    11701 aagtgcagca gcggattgag caatggaaaa gaaaggaaag tgacaaaatg aatttcggac
    11761 aaaattt
//
LOCUS       DACECY010000076        11761 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.081, whole genome shotgun sequence.
ACCESSION   DACECY010000076 DACECY010000000
VERSION     DACECY010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.081"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(419..2428)
                     /gene="metG"
                     /locus_tag="IWO38_002333"
     CDS             complement(419..2428)
                     /gene="metG"
                     /locus_tag="IWO38_002333"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ0314402.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            2696..2974
                     /locus_tag="IWO38_002334"
     CDS             2696..2974
                     /locus_tag="IWO38_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314403.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            3071..3892
                     /locus_tag="IWO38_002335"
     CDS             3071..3892
                     /locus_tag="IWO38_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ0314404.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(4177..5187)
                     /locus_tag="IWO38_002336"
     CDS             complement(4177..5187)
                     /locus_tag="IWO38_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0314405.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(5199..6152)
                     /locus_tag="IWO38_002337"
     CDS             complement(5199..6152)
                     /locus_tag="IWO38_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0314406.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(6142..7212)
                     /locus_tag="IWO38_002338"
     CDS             complement(6142..7212)
                     /locus_tag="IWO38_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0314407.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(7218..8261)
                     /locus_tag="IWO38_002339"
     CDS             complement(7218..8261)
                     /locus_tag="IWO38_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0314408.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(8262..9203)
                     /locus_tag="IWO38_002340"
     CDS             complement(8262..9203)
                     /locus_tag="IWO38_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0314409.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            9287..9634
                     /locus_tag="IWO38_002341"
     CDS             9287..9634
                     /locus_tag="IWO38_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ0314410.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(9704..11365)
                     /locus_tag="IWO38_002342"
     CDS             complement(9704..11365)
                     /locus_tag="IWO38_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0314411.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         3358 a         2370 c         2117 g         3916 t
ORIGIN      
        1 atctatattt tcaatcacac atataagagc acaaactaat tgaacataaa gtgatagaac
       61 ttataattaa cacatcaacg ctactgttag atataaatat agatgtaata aagtcattgc
      121 attcaaactt tatgaatgta aggacaataa aaaagagtac cagatgaatc aattaaaatc
      181 tgcccgaccc tttatcatta tgcttttttt ctgtttttac gattcctatt agtattattc
      241 taaattaaca aaaatcagcg aagctcgtac ttctttttct aggatttatt ttggatagtt
      301 ctctgtttct gtattatatg attggcttca agtaataaga aggaatttac gaaaattgct
      361 tctcaaattt cggaaaaagg gggtatgaca caactagtgt cataccccct ttgaatttct
      421 atgcgatttc tgagccattt ggcatggatt ttggtgcttc gattacttgt aaagagccat
      481 ctggtgcttc tgcagaaagg atcattcctt gactgatttg tccacgcatc tttcgtggtt
      541 tcagatttgc tacaatgacg acttttttcc cgatcaattc gcttggatca ggataaaatt
      601 cagcgattcc tgacaagatt tgacggtctt ggctgtctcc agcatctagt cggaattgca
      661 gcaacttatc tgccccttct actttttgac agttgatgac ttctgctact tttagttcta
      721 ctttttcaaa tacatcaaat ttgatttgtt tttctttcgt tgaaacaagt tctgtttctt
      781 ctggatccca tttgactgta tcttcgtttg tttgagtacc ttcagacatt ttttcttgaa
      841 taaaagcaac ttcttcttcc atatctaaac gtgggaagat tggtgtacct tttgcaacta
      901 cttttgttcc tgaaggaaat tcgccaaagt gtaatccttc aagattcatt gtttcagagt
      961 ccaatcctag ttgattgaag atcttctctg gcgtttctgt catgattggc tgaagtaata
     1021 tcgctgcgat acgcaaagat tcagctaaat ggatcatgac actattcaat tcagctttct
     1081 tttcttcgtc ttttgctaat acccatggtt ctgtttcatc aatgtatttg tttgcacgag
     1141 aaatcagcac ccagatttca gccaatgctg tattgaattc cattttttcc attgcatcat
     1201 ggtaacgtcc gacaacgtta gcagccgtcg tagacagttc actatcaaat ggagtgacaa
     1261 gtgaggcata gtcaggaacg agtccatcac aatatttgtt gatcatagca atcgttcgat
     1321 tcaataagtt ccctagatca tttgccagat cataattgac acgggaaaca aaatcttctg
     1381 gcgtaaagac accatctgac ccaaacggaa cagcacgaag caaataataa cggagtgcat
     1441 ctaaaccata acgttccaca agcatttcag gataaacgac atttcctttt gatttagaca
     1501 tttttccgtc tttcatcaat agccagccat gcccaaatac ttttttaggt agaggcaaat
     1561 caagggccat cagcatgatt ggccaataga tcgtatgaaa gcgaacgatt tctttaccga
     1621 ccatctgaac atctgcaggc cagtattttt cgaatagact ttcatcttcg gagccatatc
     1681 ctagtgcagt aatataattg gataatgcat cgatccatac atacaccaca tgtttcggat
     1741 cactcttgac tggtactccc caagtaaaag ttgttcgtga aactgcaaga tcctctaatc
     1801 ctggtttgat aaagttgttg atcatttcgt ttttacgaga ttctggctgg atgaattcgg
     1861 gatgttcttc atagtattcc aatagacggt ctgcgtattt gctcatgcgg aagaagtatg
     1921 attcttcttt taccaattct acttcgtgtc cacttggcgc tttaccaccg atcacgttac
     1981 cttcgtcatc tttgtacact tctgcaagtt gtgtttctgt aaagaattct tcgtcagaaa
     2041 cagagtacca gccttcatat tcacctaagt aaatatctcc ctgttctaat aaacgatcaa
     2101 atattttttg aacagctgct ttatggtaat catctgtcgt gcgaatgaat ttatcataac
     2161 tgatatcaag cgttttccat aatttcttga catcagccgc cattttatct acatattctt
     2221 gcggttttac acctagttct tctgcttttg tttcgatttt ttgaccatgt tcatccactc
     2281 cagtgagata aaacacatca aagcccatca accgtttata acgagcaatt gcgtcacatg
     2341 caatcgtagt atacgagttg ccgatatgaa gtttgccgct tggataatag attggcgtag
     2401 tgatataaaa agtttccttt tctgccacgg ggctcttcct cctttatcgc atgatgcgaa
     2461 ttcgtttcct tgctagtata ccataaaaaa agcatgctgt tagctttttt tcaaaatgaa
     2521 aaagactgga taaacgactt ctatctacgt tcaatctatc ctttaaaagg atgaataggt
     2581 tgaataaatg cgggtttttg ttttgtttca tatctactaa aaaacggctt tttatttctc
     2641 ccctcacacg tggtataata aagactatct atcttagtaa gggactggaa caatcatgca
     2701 gaagaaacat ctttttttta cacttagtat tgcttttctt tcattagcac atttaatatt
     2761 cagctatttt tatattcgta tgtatggtta ttttaacctc catgggcatt tgaacagttt
     2821 tatgacagca gcttggattt tgcgttttat cattgacgta tatatcgtta tttgtggctt
     2881 ctttgccatt cgtgaagagc ggtataaagt attgccgttt tatctgcttt tctttttatt
     2941 caatctcata cttccattta tttttcatat ttaaatgata acctagattt ttcaagtaaa
     3001 tccttggttt tttttcagag tctgctacaa taaggtaaaa agtactttta ctgtcaatgg
     3061 aggtggtcag atgaaccgtt ttgcttctaa agaagaagaa aaattatatt atgaacataa
     3121 agcaagtgaa gaagaatttc ttaactggta tcaaaaacag gaacgtccag aatatgacaa
     3181 gccctctttg actgtcgata tcgtactgat gtgctataac aaagaagaag atcagctgaa
     3241 agttttgttg atccaaagaa aagggcatcc ttatcgaaat tcatgggcat tgcctggtgg
     3301 ctttgtccaa aaggatgagt caactggaga aagcgtctta agagaaacaa aggaagaaac
     3361 tggtgtggtt atttcaaaag aaaatattga gcaactgcat acattcagta cgcctaatcg
     3421 agatccgcga ggctgggtcg tcacagtcag ctacttagcc tttattggag aagaaccgct
     3481 aatcgccgga gatgatgcca aggaagtacg ctggttcaca ctagaacgtc atagtaacaa
     3541 gatccattta tctagcggag aagtggctat ctcgcttgat ttagtcactg gagagtcttc
     3601 tggcaaagat actttggctt ttgaccatag tcaaatcatc ttgaaagcat tcaaccgtgt
     3661 agtcaataaa atggaacacg agccgcaagt tctacaagtt ttaggtaaag atttcacgat
     3721 tacggaagct cgaaaagtat ttgctaaatt cttagggatc gattataaaa cgatcgatca
     3781 ttccaatttt aagaaagcta tgctccaata tttcgatgag atcggtgaac gtcctgtagg
     3841 aatcggccgt ccttcaaaaa tctatcagct caagccgggg caccatgaat aaggttgtga
     3901 gcaaaagatt cagacctaat cattcaagaa aaaacgcaaa aacaactctt tgtgagttac
     3961 gttttttgtt tcagaatttt gatgtattcg taaactttct gaataaaaaa ttaacaatag
     4021 cagaaaaagc tcgtacgaac tttttctgct attgttttat tggtttgtca taattagtga
     4081 gcttttcttc aaaagaaaaa ttcctagaaa caaaagtcat taagactttt tattaacaag
     4141 gctaacccta acgctcctat cagtttcatt cattttttat cccttgataa ccgaatgtct
     4201 gcccattttt tctactactt ggctctttct tacggaattg ctttcttctg ctccgtcggt
     4261 attgatcaaa agaaattttt tattatacca ttgggactta taacgcaatt ttgttattaa
     4321 tttatgccag tcgtgaatag aaaaatcagc atgtgcctct tcttttggaa aagattctac
     4381 aaataagtag tcatattgtt tgaccaattt cccagataat tgttcaagat aatcttctcg
     4441 ctggttcttt tgtttcagat aaagatccat tacttttctc ttttgtttct gataattgct
     4501 tgcctgatct aacctgatct ttctatggtg agcggagcgg actttcacat tcaatttccg
     4561 ctgtgcatgt tgcagctttt cttctgtctg gtgaaatttt ggtaaagtta cttctagagg
     4621 tcgagatgtt tggatcaatg tcttcggatg ataggcgatc ccgatccatt tagctaaaga
     4681 ttcttttttt actggcgaaa catcacaaag tagtgcgaca taaaattctt cattatatcg
     4741 agcagaaatc gtagcggagc gaatcgatcc ttcaactggc cgatgctgat ttatcttgat
     4801 tagttctttt tgttttggta actttaagta gccattttcc agatagatcg tacgggtttg
     4861 gttgttggtc gtataagatt gccacatact ttttttcgtt ttaagctttg ggaagctggc
     4921 acgtcccttg aaataattgc gaaaagcccg atccaaatta agctgtgcat ttgctaaagc
     4981 aagactgtct acttctttta agaatgggta ttcttttttt aatgtagcgg gtgtgagcga
     5041 aaaattggct actcctgttt gttgccaact tttctgccgc tttttcagca aatggttata
     5101 ggtaaatcga gcacatccaa aagtacgaat caattgctgt ttttgttctt ctgtcggata
     5161 catacggaat ttaaaagctt ttagcatcta tgtccctcct agttagcaac cagaagagat
     5221 tccttttgtc gttttagttt ttttgcgtaa tatgggactt cctgctcagt tgcatagaca
     5281 aaatgttctg gtgcgatttc acgaagttga cgtacttgtc cgtcatctgg ttcaggttga
     5341 tatttgattc ttctgcgttt tctctcatgg tctggatctg gtagaggaat agcagataac
     5401 aaactttctg tataaggatg aacaccgtga ttatagattt catcacttgt tcccatctct
     5461 aataacaagc cgttatgcat tacaccgatt cggtcactaa tatgtttaac catcgacaaa
     5521 tcatgtgcaa taaatagata ggtcaattga tgttcttttt ggagatcttg caacaaattg
     5581 accacttggg cttggatcga cacatctagt gcagaaattg gttcatcaca aatgataaaa
     5641 cgcggtttta ctgccaatgc tcgtgcaata ccaatacgct ggcgttgccc acctgaaaat
     5701 tcatgaggat aacgagtacc gtggcttgga ttcaatccaa cagttctcaa tagatcatcg
     5761 actttttttg cacgctcttc agatgattta gctagtccat taacatcaat tccttcggca
     5821 atgatatctc tgactttcat acgaggattc aacgaagcgt atggatcttg aaagatcatc
     5881 tgaacatcat gccggaattt cgtcaaacct tttttatctt tgatcttcgt tatatcttga
     5941 ccgtcgaata atatctcacc atcagtaggt tcattcaagc gaatgatcgt acggccagta
     6001 gtggattttc cgcttccaga ttctccaact aaaccgaatg tttctccttc ataaatgtga
     6061 aagctgatgt catttacagc atgtacttca ttcttttttc ctacattgaa atactgtttc
     6121 aatcctttaa cttcaagtag tttatttggc atcagcaatt cctccttgac cgtaagtatg
     6181 tttttgtttc tctttaaaga tagcccagcg acgttggatt tcttctggcg gtgtcacttt
     6241 gggtgcctgc ggagcaagta gccaagttgc tgccttgtgt gtttttgata gttcaaaaaa
     6301 tggtggttct tgttctgcat cgattttcat cgcgaattca tttcttggat agaatgcgtc
     6361 tccttttggc gggttcaata aatccggtgg agaaccagga atagcataca atcgatcgtt
     6421 cgcactatct aatgtcggca ttgaaccaag cagtccccaa gtatagggat gttgcgggtt
     6481 gtagaaaatt tcttccgatg ttccaacttc aacgattctt cctccataca tgacagcgac
     6541 acgatcagcg acattcgcta ctacaccaag gtcatgagtg ataaagataa ttgatgtatc
     6601 gatttttgtc tgaatttctt tcagcagctc caaaatctgt gcttgaatag tcacatcaag
     6661 cgctgttgta ggttcatcag caatcaggat ttccggataa cataccagtg caatcgcgat
     6721 cacgatccgt tgacgctgac ctcctgagaa ctgatgcgga taactgttga tacgtttctc
     6781 agcttcgggt attccaacta atttaagcaa ttctaatgca gctttttgtc cctctttttt
     6841 tgaaacttta ttatgctttc taagggactc agcgacttgc ttcccaatgg gcatcgtcgg
     6901 atctaaagag gtcattggat cttggaaaat catcgctatt tctttaccac ggattttttg
     6961 catctctctt tctgattttc caacgatatc gttaccttta aagaggattt gaccagcatc
     7021 tatattggcg ttgcttgaca gcaagcgcat gatgcttctg gtcgtcactg attttccact
     7081 tccagattcc ccaacgatcg ccaatgtttc tcctttattc aaatgaaaat tgacaccgcg
     7141 aatcgctcgt acctttccag catacgtatc aaaagagatt tctaagtctt tcacttctaa
     7201 aattttcgtc atcgtcttca ctctttcatt ttaggatcaa aggcatcacg caatccatca
     7261 gcaagtaagt taaagccaat catgataacc gataaagtta ctgccggaat ccataataga
     7321 taaggcagat acaggaatgt cttgtaccct tcgctcaaca tcgttcctaa tgaagctgac
     7381 ggaggcgtaa gacctaaacc gataaagctt aaaaaggctt cgaagaaaat agcagatgga
     7441 atactaaaca tcatttgaat aataatgaca ctagagatat tcggcaaaat atgtttgaat
     7501 gcaattttca ttcttgattc acctaatgtc aatccagcta aaacatattc ttgatctttt
     7561 aatttcattg tttgtgcacg aacgatacgt gccattggta tccagtttgt gatggccata
     7621 gcagccacga ttgaaaaaat tcctggttca aaaacgacca acatcaaaat catgacaacg
     7681 aggtttggaa ttcctgataa gatttccaaa aaacgttgca tcacgttatc gacacgtccg
     7741 cctaacaaac cagaaaccaa cccgtaagct acaccaatcg ttacatctag taatgcagca
     7801 ataaatgcga tcagcaacga gattctcatt cccataaata agcggcttaa tacatcacgt
     7861 cccaaaccgt ctgttcctaa aaagaaattt acgtttgaag ggacgtttgc ttgagcatat
     7921 ttatcaacaa gttcaccacc aaccattgct tttccattca atccatcgat attgattccc
     7981 ggaatacgag gaggcaaatt gatataagcc acgttttggg cagttggatc atgaggagaa
     8041 acaaagattg tgatcacaga gatcaaaatg atgaccaata gtaaaaccat tgaaataacg
     8101 gcagctttgt ttttctttaa acggcgccat gaatcttgga taaaattcaa agaaggtgca
     8161 gtaatctgtt cacgttcttc ttcggtattt ttttgtaatg gaagaaattc atccgcaggg
     8221 atatctgcaa ctgtttcata acgctttggt atcatttcca tttatcctcg gctcccttct
     8281 gaaacacgaa ttcttggatc aactagaccg tacaatagat ccacgatcaa aataacaaaa
     8341 atcagcataa atgagtaaag gatcgttaca gccatgatcg taggataatc atttgttgtg
     8401 attgacttaa cgaattgttc cccgatacca ggaatagcaa agatattttc taccaccagt
     8461 gatccggtca tcaatgctac agctaacggt cctaataaag tcatcaatgg aatcaaacta
     8521 tttcgaagac catgtttaaa ggcgatttcc caacggctca atccttttgc ttttgctaat
     8581 tccacataat cactatgcag tacttccacc atttccgtac ggataaaccg agcagagtca
     8641 gcaagcggcg acatggccaa cgcaattgtt ggtaagattg tgtacacaaa gccatcccat
     8701 tttgcgattg gcaaaacttg taatttgatt gcaaagatat attgtaataa aacagcaaat
     8761 acaaagtttg gaatggaacg acctaatatc gctacgagtg tagaagatgt atctgcccat
     8821 gtattttgct tcatggcaga gatcgttcct aaaatcgtcc ctaggactgt tccaaagata
     8881 attgcctgta accctaactg caaggatggt ccgattcttc cagctaacaa atgtgcgact
     8941 ggctggtttt taaattggaa ggaaattcca aaatctcctt gaagcaaatt agaaagataa
     9001 atcccatatt gtacgataac tggtttatcc aaaccgactt gtttatttag catctcgatt
     9061 gtttcaggac tcaaacgttc ctgattcgta tatggcgtgc ccggcagcaa ttgcatcaag
     9121 aaaaaggtga ttgttgcaat taaccaaagc gtgatgatca tgaaaaagac acgttttaat
     9181 acatatttga tataactatt cactaatgtt tccccctagc ttttgacttc tgacagatgt
     9241 ttcgctacat tattagataa tgaaccaaaa ataaaggagc aatcaaatga aaaaaaaatc
     9301 tatcccctac gcagtagctt ttctactgat tctggttatt ttaataaaaa atctcatcaa
     9361 ccattccttt acactcattc aattatctaa tgatctattc ttatggtcat tgccgtttct
     9421 gattatcggc ggttttttat gggtcttttc ttcgggtttc ttcgatcatt ttcagcgatc
     9481 ggtccatctt gcacgaacaa ggaaccgaaa gaaaaaacca gaattttcct cgctctcgtc
     9541 tgccagctac ggtatgtaca gcttttggct gatcattgcc ggaatactaa ttgctctttc
     9601 tgctattttc atgctttttt ctttgttagg ataagtgaat atgagaacga ggttgcgaca
     9661 tttgccgcag cctcgttttt tctcatgctc acggtattaa cgattattct tcaacgtatg
     9721 tccatttgta atcccatgaa gcgcctgccg catggtgaac gatacctttg attttttcat
     9781 tcactaggtg accttctgct ttttggtaca ccggaacgac acccatatca tcagagatga
     9841 tcttgattgc tttttggaaa tcatcccaac gggcttgttc atcgccagcg tctgttgatg
     9901 aagctgcttt aactgcttcg tcatattctg cattgctcca gcgaccacgg ttgtatgaat
     9961 tacctgtgac aaacaaatca gtaaagctgc ttgggtctgc atagtcagca gcccatccgc
    10021 ctagtactgc gtcaaaatca cccgaagttg agcggtcaag acgtacagag aatggaacag
    10081 gtgttggttt gactgttacg ccatccaagt tttcttggat tgagttttga acatattcga
    10141 ttactttttt cgatgaatca tcatcagaag ccatcaaatc aaattctaaa gaatcaacgc
    10201 ccaattcttt tttcgctttt tcccaatatt cttttgcttt atctttgtca taagaaacta
    10261 attttccagc ttcttttgca aagtcttcgt tcgttgttgg actttttacc atatctgatg
    10321 ggatcaatcc tgtagagaca actgatccgt cacctagtac ttgttttact aaagcttcac
    10381 gatcgataga ataagaaata gcttttctta ggttcacatt gttgaatggt gaattctttt
    10441 cacgttgatt gaattcgata tagcttgtcc gcgcttcttt aacagaagta taagctggat
    10501 cgtttgcgtt ttgctgtgct aactcaccag acaagatcac gtcatctgct tggccatctt
    10561 tgaataagtt caatgcagta gaagattctt ttactacact gactttgatt tcagaaagtt
    10621 ttactgtatc tttgtcccag taatgatcat ttttcttata agtccattct gtatctgttc
    10681 ctggtccatc aaaatcttct aatgtgaatg gtccattata aacagcttta tcacttgcag
    10741 atgcataggc atccccattt tcttccacta cggattgttt ttgtgggaaa aatgatggga
    10801 aagcaagcag atattggaaa tatggtgttt gtttcgtcaa tttgatttct aattcatagt
    10861 caccaactgc tttgatccct agttctgatt tatctttctt tccagctgta atgtcttctg
    10921 cattttcaac tggcgcatac aggtaagcat attcagaagc tgtagctggg tctactgttc
    10981 gttgccagcc ataaacgtaa tccgctgctg taacaggatc accatttgac cattttgcgt
    11041 cttctcgtaa tttgattttg taagttaatc cgtcttcact gacttctgcc aactcgctag
    11101 ctcctgccgg ctgtggtttg ctgtcttcat caagacggta aattccttca taaacgttat
    11161 tcaatgctgt aaaactgatt gtatctgttg caactgataa gtcagctgta ggcatttctt
    11221 gaggcacgac taagttaaat acgcctcctc catctgctga accgcttttt gctgaactac
    11281 tgcttgtggc tgaactgccg ccataacatc cagcaagtac aaggccgcat accgcaacca
    11341 aaccaaatgt aaatttcttt ttcattttac gtaccccttt attggaattt tcagaataat
    11401 taatattttc tgaagttgtt taagagttta atgaataatt attaaaaaat caacactttt
    11461 ttttgtcttt ttcataaaat aatgattttt tcgtgaggtt tcaaggaatt tttcagaaaa
    11521 aatagctttg tacaaggttt tccatggtct tccaaaagcc attaaataat tttcgttata
    11581 ttgtctatta ttgccataca aagataaaaa agaataaaca aaaagaaact gtgacaaaag
    11641 tcttgtcaca gtcccttatc tgaataaacg gtgttcaaaa gccagtttct cggtattaag
    11701 ccaaatttat gcaaaaatgt agaaagccat tttcacaaaa tttttcttac tactcgaaac
    11761 t
//
LOCUS       DACECY010000077        10952 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.082, whole genome shotgun sequence.
ACCESSION   DACECY010000077 DACECY010000000
VERSION     DACECY010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.082"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            38..126
                     /locus_tag="IWO38_002343"
     tRNA            38..126
                     /locus_tag="IWO38_002343"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72..74,aa:Ser,seq:gga)
     gene            141..212
                     /locus_tag="IWO38_002344"
     tRNA            141..212
                     /locus_tag="IWO38_002344"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:174..176,aa:Glu,seq:ttc)
     gene            228..300
                     /locus_tag="IWO38_002345"
     tRNA            228..300
                     /locus_tag="IWO38_002345"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:261..263,aa:Val,seq:tac)
     gene            310..382
                     /locus_tag="IWO38_002346"
     tRNA            310..382
                     /locus_tag="IWO38_002346"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:343..345,aa:Asp,seq:gtc)
     gene            418..493
                     /locus_tag="IWO38_002347"
     tRNA            418..493
                     /locus_tag="IWO38_002347"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:451..453,aa:Phe,seq:gaa)
     gene            495..575
                     /locus_tag="IWO38_002348"
     tRNA            495..575
                     /locus_tag="IWO38_002348"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:529..531,aa:Tyr,seq:gta)
     gene            582..655
                     /locus_tag="IWO38_002349"
     tRNA            582..655
                     /locus_tag="IWO38_002349"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:614..616,aa:Trp,seq:cca)
     gene            658..730
                     /locus_tag="IWO38_002350"
     tRNA            658..730
                     /locus_tag="IWO38_002350"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:691..693,aa:His,seq:gtg)
     gene            742..813
                     /locus_tag="IWO38_002351"
     tRNA            742..813
                     /locus_tag="IWO38_002351"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Gln,seq:ttg)
     gene            859..929
                     /locus_tag="IWO38_002352"
     tRNA            859..929
                     /locus_tag="IWO38_002352"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:891..893,aa:Cys,seq:gca)
     gene            950..1034
                     /locus_tag="IWO38_002353"
     tRNA            950..1034
                     /locus_tag="IWO38_002353"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:984..986,aa:Leu,seq:caa)
     gene            1096..1168
                     /locus_tag="IWO38_002354"
     tRNA            1096..1168
                     /locus_tag="IWO38_002354"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1129..1131,aa:His,seq:gtg)
     gene            1178..1249
                     /locus_tag="IWO38_002355"
     tRNA            1178..1249
                     /locus_tag="IWO38_002355"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1210..1212,aa:Gln,seq:ttg)
     gene            1267..1351
                     /locus_tag="IWO38_002356"
     tRNA            1267..1351
                     /locus_tag="IWO38_002356"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1301..1303,aa:Leu,seq:caa)
     gene            1729..2481
                     /locus_tag="IWO38_002357"
     CDS             1729..2481
                     /locus_tag="IWO38_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0314412.1"
                     /translation="MGKGIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPI
                     EFMDFAEEVIELITARISSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLY
                     PNEYDIAIEVVERINHRYQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTIL
                     SHLEKSYSNVYDKESLTYHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCT
                     ALKIKDIIEQQFQLAIQENEIIYLAIHLARIY"
     gene            2585..3349
                     /locus_tag="IWO38_002358"
     CDS             2585..3349
                     /locus_tag="IWO38_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ0314413.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            3366..4778
                     /locus_tag="IWO38_002359"
     CDS             3366..4778
                     /locus_tag="IWO38_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0314414.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAIFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            4795..6119
                     /locus_tag="IWO38_002360"
                     /pseudo
     CDS             4795..6119
                     /locus_tag="IWO38_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            6132..6590
                     /locus_tag="IWO38_002361"
     CDS             6132..6590
                     /locus_tag="IWO38_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0314415.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            6882..8183
                     /locus_tag="IWO38_002362"
     CDS             6882..8183
                     /locus_tag="IWO38_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0314416.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEKAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNATLLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPMIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            8210..8470
                     /locus_tag="IWO38_002363"
     CDS             8210..8470
                     /locus_tag="IWO38_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ0314417.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(9469..10704)
                     /locus_tag="IWO38_002364"
     CDS             complement(9469..10704)
                     /locus_tag="IWO38_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ0314418.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         3340 a         1768 c         2350 g         3494 t
ORIGIN      
        1 ctcattttga gaaagagtat tgagcttctt tcactctgga gagttgtccg agaggccgaa
       61 ggagcatgat tggaaatcat gtagatggtg caaactgtct caagggttcg aatcccttac
      121 tctccgtagc aaatttacct ggcccgttgg tcaagcggtt aagacaccgc cctttcacgg
      181 cggtaacacg ggttcgaatc ccgtacgggt cattttaatt gaatgttgga ggtttagctc
      241 agctgggaga gcatctgcct tacaagcaga gggtcagcgg ttcgatcccg ttaacctcca
      301 taatgaactg gttccgtggt gtaggggtta acatgcctgc ctgtcacgca ggagatcgcg
      361 ggttcgattc ccgtcggaac cgtcgttctt gttattcaat taataatttt aaaatatggc
      421 gcggtagctc agttggtaga gcaacggatt gaagctccgt gtgtcggcag ttcgattctg
      481 tcccgcgcca ccatggagga gtagcgaagt ggctaaacgc gacggactgt aaatccgttc
      541 cttagggttc agtggttcga atccactctc ctccatttta taggggcata gtttaaaggt
      601 agaacagcgg tctccaaaac cgttagtgtg ggttcaattc ctgctgcccc tgccatggcg
      661 aatgtggcga agtggttaac gcaccggatt gtggctccgg cattcgtggg ttcgattccc
      721 atcattcgcc ctttttattc ttggggtata gccaagcggt aaggcaaggg actttgactc
      781 cctcatgcgt tggttcgaat ccagctaccc cagtttctat aatttattct ttattttgta
      841 gattgataat agtataatgg cggtatagcc aagtggtaag gcagaggtct gcaaaacctt
      901 catcaccggt tcaaatccgg ttaccgcctt agtactaata aatcaacttg ccggcgtggc
      961 ggaattggca gacgcgctgg actcaaaatc cagtgcccgt tgagggcgtg ccggttcgac
     1021 cccggccgcc ggtattgact aagatgtctt catcttagtt ttttattgag aaattaaata
     1081 acatctttta tgatggcgaa tgtggcgaag tggttaacgc accggattgt ggctccggca
     1141 ttcgtgggtt cgattcccat cattcgccct ttattcttgg ggtatagcca agcggtaagg
     1201 caagggactt tgactccctc atgcgttggt tcgaatccag ctaccccagt tattattttt
     1261 aaatttgccg gcgtggcgga attggcagac gcgctggact caaaatccag tgtccgttga
     1321 ggacgtgccg gttcgacccc ggccgccggt ataattatca atatagcaac tttcttatag
     1381 agagttgcta tatttttttt gtttttatgt aattacctcc aaatcaattc gatacaagta
     1441 ttatcataaa acgagtaaaa aagatattga taacgctttt attatgtgtt aaactatttt
     1501 tgtaaattat gtagggtgct actattcaat taggcacgac ctaagaaagg gttctttctt
     1561 aggtcgtttt ttgtctttaa aaattgtttg gagacaaaaa tatgtatgaa ggagtctgca
     1621 agttgttttg ttttatcata tagtggaggt gaattaacgg tgtatgtgat gaaaaagctg
     1681 aatcataatg ctgtattgat cgatgacgat ggaatagaat aaattgcgat ggggaaaggg
     1741 attggctttt ctgctaaagt gagtaagcca tttgatgttg aggatgctga caaattattt
     1801 gtattagatt ctaaagaaaa gatcaaacga ttcagtgaga tagtctcgca gattccaatc
     1861 gaatttatgg attttgcgga agaagtgatt gaactgataa cagctaggat ctcctctcca
     1921 ttagattcaa atatctatat tacattgaca gatcatattt attttgctat tcaacgaaaa
     1981 agagaagata gagatgtcac agcaattatg ttgccagaaa tgagattcct ttatccaaat
     2041 gaatatgata tagcaataga agtggtcgag cgaatcaatc atcgatatca aaccgagtta
     2101 gggtcaaatg aagtaggatt catcactatg catatcatta atgctgagct aggagagcct
     2161 aatagtttaa agagcttaaa aatagtagaa atgacgacca cgattttgtc ccatctagaa
     2221 aaatcctatt caaacgttta tgataaagaa tcactgactt atcatcgctt aatggttcat
     2281 atcaaattcc tggttaaacg cttgatttat caggaagaaa cgccagtaga aaatattggt
     2341 ttttttactg aaacatttaa acatagcaaa gcgtattgta cagcactaaa gattaaagat
     2401 atcatcgaac agcaatttca acttgccatt caagaaaatg aaatcatcta tttggcaatt
     2461 catctagcac gaatctatta aaataagggc gtaactatct ataggcgcaa ccaaatgaca
     2521 catagtaaat ggactatgat tatttggttg cgcctatttt agtagaaaaa agaaggagaa
     2581 tgaaatggga aaagtgattt ttaattcgga tgattttggc tatagccatg gtgtgaatta
     2641 tgggattatg gatgcttatc agcgaggaat tttgacatca actacattga tggcaaatat
     2701 gccgggtttc gagcacgctg tgaaactaag aaaggaaatg cctaggctag gtgtaggagt
     2761 ccatttaaca ttgacgtgtg gcaaaccact tctcaaaact gtcgatactc ttatcgaaag
     2821 cggagagttc agatccttat cttattatca gcatccattt aacattgaca aagatcaact
     2881 gtatcaggaa tggaatgctc agattcaaaa aatttatcat gcggggatca taccaactca
     2941 tttagacagt catcatcata ctcatacttt ggatgaaaac caggaggtag ttgttacatt
     3001 agcaaaaaaa tatgatttgc cggttcggag caactttgaa cgaaaagatg aagttcgtca
     3061 tgtcgattat tttgagcctt gtttcgatgt cgtaggagaa actgatgaaa aaaaacgaca
     3121 aatcaatacg agtgtggaag aatatttggc aagtttactc atgatattga gagaagataa
     3181 aacgatcgaa gtcatgtgtc acgcagcgta tttggatcag gaattgttaa atggttcaag
     3241 ttttgtgtat cctcgtactc accaagtcga atttttaatt cattcagcat ttgctcaaaa
     3301 agtgaaaaat gatcctttga ttgagttagt aacatatgag gatatttaat agaggaggaa
     3361 taaaaatgaa tgttaaatca aatgtgcaga agcttggaag agcgatgcta cttccagttg
     3421 cagcgatgcc attagcggga ttgatcatgc gtttatcagc tgatgatatg ctgaacatcc
     3481 cagtaattgg tgcagctgga aatgccgttt ttggaaactt ggatatctta tttgctatag
     3541 gagttgcaat cggttttgtc aaaaataagg acaaaggaat acctgcactt actggttttt
     3601 tagcgattgc taccttgaaa aaaggcttag aaatcatgga cccagaagtg aatatgggga
     3661 tttttggtgg aattatttcc ggtttagtag cggcatggac gtataatcgc tttaaagaac
     3721 aaaaattacc agtagttttt tcttattttg cgggcgaaaa attcccattg actatggtga
     3781 tgattctaca aacaatcgca gctatttttt tgggctttat ctggccgctc atccaaattg
     3841 ggattgataa ttttgcaaaa ctgctcgttg atatgggggc attaggtgtg gggatattta
     3901 tgtttttgaa tcgtttgctg atcccctttg gactgcacca tgtgttgaat acgtatattt
     3961 attacgattt aggaagctat acatctccta gcaaggaagt tttcagagga gagatgacac
     4021 gctttatcaa cggagatcct caggctggac tgtttttatc aggttttttt gtagtaatga
     4081 tgtttggtat accagctata tgtctagcta tctataaagc agcttataaa gaaaataaag
     4141 agatggtcaa aggaatcatg ggaagcggtg caggaacttc atttattgca aatatcactg
     4201 agcctgttga atttagtttc atgtttcttt ctcctatgtt gtacgtgatc catgcacttt
     4261 ttgccggttt agcagggatt gtctgttact tattcaatat acgaattggt tttacattcg
     4321 gagcatgtat tgttgattat ctcatcaatt ttcgaatcgc taccaatgct attttgattc
     4381 ttccaatcgg aattttcttt tttgccttgt attacgttac tttttattat ttgatcaata
     4441 agagaaacat tcagacatta ggacgtgaag ctaaggcaga gtttggcaat gaagtaacct
     4501 tagaagaaac agagttaggg ttagcaagta aaaattatga atatatggca acaaaaatgc
     4561 tacaagcgtt tggaggaaaa gccaatatcc tggatgtcta tagttgtaat acacgcctac
     4621 gggtagaggt agtggatccc accatggtcg aggaacagcg aatcaagcaa ttagggataa
     4681 gcgggatcat taaaccaact gaaaaaaatt atcaaattat catcggctta gaagtgactt
     4741 atgttatggc agaattcaat aaattattag aagaatagaa aggaaaagat gatgatgaaa
     4801 aaacagatta tcactatagc tggaggcggg agtacgtata caccaggaat cgttcaagcc
     4861 attttgaaca atgaaaaacg gttgccatta tctgaaatac gtttatatga tattgatgaa
     4921 gaaagaaata tggatatgta cttgattgtt aaatttatgc tgaaaaaaaa ggtttttcaa
     4981 atattcgcat tcgtgcaacg gatgatccaa agcttgcttt tacaggatgc gattttgttt
     5041 tctcacaaat tcgtgttgga ggattggaga tgcgagaaaa ggacgaaaag attccactta
     5101 aacatggatt agtaggacag gaaacgtgtg gactcggagg atttgcttat gggatgcgct
     5161 caatcaaagg tttattggaa attgtcgatc atatacaaga ctatgccccc aaagcatgga
     5221 ttttgaatta tacaaatcca gagtcgatcg tttctgaagc tgttcgtcgt aaatatccga
     5281 atgtaaaaat gattaatgcc tgtgatatga ctatttcaat tgaagaaacg attgcagtga
     5341 attacggtta tgatcgtaag aattggatcg ttacgtatta tggtttaaac cactttggct
     5401 ggtatacatc gatttatgat aaagaattgc aacgagaaat tatgccagag attattgaaa
     5461 aactaataac aaaagaaatg caagtcgccg attttaatat aggagacaaa acttggcaaa
     5521 aaacctttca aatgatgtca gtcatcacga aaaattttcc aagtaatatc ccgaataatt
     5581 acttagagta ttacttatat cctgatatgg tcgttgagca tactgacaaa gagtatacac
     5641 gtgctaatat ggtcatggat ggacgagaga aaaataccaa agaaatggct gataaaatac
     5701 gccgcggtat tgtggaagaa gtattgaact ttaattttgg ggagcatgga caatatatcg
     5761 tcgatatagc tatttccttg ttgaatgatg atcgtcgtcg ctttatgcta atcgttccta
     5821 atcaaggggc gattccgaat ctacggtcag atgcagttgt tgaaataccg gcatatgttg
     5881 gtgcaactgg tgtggagaca attacactaa gaaaaccaat cagtgatttt cataaaggat
     5941 tgatggaggc acaagttgca gcagagaagt tactagtaga tgcttacttt gaaggttctt
     6001 atcaaaaagc tttacaagca tttacattga atcaaaccgt accaaatgcc agagtcgcaa
     6061 aaaaaatact agatgaaatg atcaaagtaa acaaaaaata ttggccagca ttaaagtaga
     6121 ggtgataaaa agtgcatttc tttaaaacaa gaaacattct aaaagctgta gctagtggtc
     6181 aaattattcc attaacagca gtaaaagacg aggtcttttc ttcaaaaatg ataggagttg
     6241 gatacgctat aacgaatcat gatggatata tccatgcgcc agttaaagga ataatcgata
     6301 atatatttcc tacccgacat gccataacga tcgaaagtga atctggcaga aaaatactga
     6361 tccatatggg tttagataca gttgaactcg aaggaagtcc gtttcagata cgggttgaaa
     6421 gtaaccagga ggtaaaagaa gggacactgt tagctattat agatttgaag aagttggagc
     6481 aagcacaaaa agatacggtg atcattgtag tttctccagg catttttgaa ggaaatttat
     6541 taaaacaaaa ccaagaagta aagattaaag atagttgttt tgaattttag tgtacgatga
     6601 aaaaagttaa atagggtgat atttatgggt gaatttacaa aatttttttg aaatgataag
     6661 tttcaatcac tagatttaat cattttgatt tttgttaatt atcgaatttt tttgttgctc
     6721 ctatgattta gaaaacttat tttcaggatt cttaaaaata aatttgatta tttaagagaa
     6781 tatattgaaa taaaatttct taattatata attagttatg aacattcttt gcgttgtcat
     6841 gcaatgaatg gttcatattt tattgaaaag gagtggtttt agtggaaaag ctgatgaaat
     6901 gggtcgaaga gaaacttgtc ccaccaatgg caaaaattgg tacacagcgt catttattag
     6961 caattcgtaa tggggttgtt tcaacattgt cattgatttt gattgggaca ttttttatgg
     7021 tctttatcaa tttaccgttt cctggatgga atgaatttat tgccccttat tcggcaacga
     7081 ttgttttacc ttttaggatg acgatgggat tgatggctat ttatgcaact tttgttatgg
     7141 gttcggatct agctaaatca tatggattag atagtgtgac aggtggtata ttatctttag
     7201 gaacgttctt tatgttacaa gttcctgtaa acgttttaac tcctgaaaag gcacctcttg
     7261 gctgggtctt acctatgagt tctttgggag catctgggat gtttgcgggg atcttatcta
     7321 tgatttttgc agtggaagta tatcgtttct ttaaaaatcg aaatattact atcaaaatgc
     7381 cagaacaagt gccacctgct gttgcccgta gttttgaggc attgattcca ggtgcagtca
     7441 ttttaacaat tacttggctg attcgaagcg tcgcgggctt tgatgtaaac gctaccttat
     7501 tatcgttatt cgagccttta accaatattt tagggaataa tttgcttggt gttttactac
     7561 caatgttttt gatccatttg ttgtggtcat ttggtatcca tggcatgagt attgtagggg
     7621 ctgttgttcg tccaatgtgg ctgatcatgt tggatgagaa tgcaaaggca ttggcagatg
     7681 gtacagctgc cacccaatta ccctatatcg ctcctgaaca gttttatcaa tggacggtaa
     7741 caattggtgg tgcaggagca acgatcgtgg tatctgtttt gtttatattc ttctgtaaat
     7801 caaaattctt gaaagaggtt ggtcgttttt ctattattcc tggtatcttc aatatcaatg
     7861 agcccatgat ttttggagct cctatgatgt tgaatcctta catgttcatc ccgttcaact
     7921 tagttccttt agttttgact attatcagtt attttgcagt caaattagaa atggtcaata
     7981 gatttactgt tttgccaact tggacattac cagctccaat cggcggttat ctttcagcag
     8041 gaaatgactg gcgtgttgtt atcttgatca tgatcaacac attgatagca tttattatct
     8101 attatccatt tgtaaaagct tatgataaaa agatgctgtc agatgaacaa agtcgtggag
     8161 aaaacgaagt ccaaactgtc taagtttggt ttttagaagg tgggaaacaa tgtattggct
     8221 gattttattt tttgttttta tctttttatt aacggctagt caccttatat tgaatatgct
     8281 agctacatat catattcaaa tcaatcgatg gatttgggca ctggcatcat ttttgatcgt
     8341 gattcttcca aagatcattg taccgcatat gaatgttttg ttttcatggg ggacatatgt
     8401 actttgtgga atttttgcta tcaattttat gatcgagcag catagatggt ttgtcacatc
     8461 gaagctttaa atgttctaaa agtggttgtg acagaagtgg ttagcttcaa gaaataagaa
     8521 gaaatttacg aaaattgctt cttaaatttt tgtgaaatgt tggcttattt ttcgtttggt
     8581 tgaacttatt accgcagatg caagcttttg accaccgttt attcggaata tgggactgtg
     8641 acaaggcttt tgtcacagcc cttttttaca taaagattat tattgtttta actgttttgg
     8701 cagaaagaag gaggcaaaaa tgaaaatttt atcagcaatg gattaagaat tagtcgatct
     8761 actaccaaag tgatcaaggc aaatatagat ttgggagctt acaaccatat agtaggggct
     8821 gcagtatggt gtatgaatgg taatatgtat gatgagagtg tttattcaaa tcatgaaacg
     8881 tgtatagaaa taattgcgct gggaatagca atcagtcatg gtgaacgtga cttttagttt
     8941 atcgttgctg acgacataga acattctgac atgatctact cagcatgtgg ggattgctgg
     9001 cagtttatat aggagtatgc atcggattgc gagatcattg tctcaacaga agagggaggt
     9061 tgattatcaa agataaagtg gtaaatccta catttgattt agtaattgtt gaagggacta
     9121 gcagcacaaa tctctacaaa gcgatgcttc tagatctata tgacacattt taagaaaatg
     9181 tatttgccta attattatgg tctttctttt gaagaaacgt tgagatgatc caaccagaga
     9241 aaggtgtcaa agaatttgac taagaataaa tggcagacgc tacctatgct catttggatt
     9301 gcgtcagatc ttttatttta aggatgattt tgtgtttttt agacaccgta atttaagtaa
     9361 ctctaacttt tcaatccact atttactttt tttgtatttt cactgtctgg gtctcgaata
     9421 gcgaaattac tgatgagtct ctattaaaaa agctttttct acttgaaact atattgggtg
     9481 gttgctcagg aaggtttcga tttcttgaat tgcttgttgt ggaccattta ttggaaaaac
     9541 ggcttgcagc gtgcgtgcct gctgacgaat cttgggtgaa gaaacttcgc gcagagcaat
     9601 ctgcagtttt ctttcagtca ctggttttgt gagggcgatt gcgtttccgt attttacaca
     9661 atagtcaata ttacagcttt gctcaaattg catccccaca ccgataaacg gtttgccgga
     9721 ttcacaggca gtttggacgg tcccttcccc gccgtggata aaagaaaaat caatgaattc
     9781 atgcaaaaga tgactagaca gatattctgt tattaaaaga tcttctgagt cagattgaat
     9841 gtcatcaagc agatagtcaa taggtgcaat aaccgctaga tccggttgtt tttgtaagaa
     9901 gaacaaaatt ttgcgtaaca tacgcggatt gcctgaacta cccattgcga aatagataat
     9961 acgttttctt tcttgttgct ttcgctgtat aaaagttagc acttcttcag gcaaggaagt
    10021 actgagctta gcaaaaattg gaccaacgat tcgataattt tccggcagct gttcttcttc
    10081 caaaaatagt ttaggcgttg tgatgagatt tagatcagca tccatcattt gtaaacttgt
    10141 ttttggtaaa ggaagcattt ctttttgtgc agcgtgtttg aaggcattgg ggatccatcg
    10201 ggtatttact atacgctttt ttgtccagtg ccaaagggga ttgcttaaag ggccgaagaa
    10261 acgtaactcg tggggcatca gcgcatgctc tgattgaaaa aaaggttcac tcaaagcata
    10321 gggtttaaca tatactaaag gaatcttttt attgcgggcc gataaaaaaa ttgtcacatt
    10381 cgaacctgtc acaatcagtc gaggctcaaa cttagcaatc agactctgtt cactagtgat
    10441 acgagaggca accatttctt tggtaaaagg gtttttaagt gtacgcaact ggtcaaattt
    10501 tatgatttgt tcttcttgtt ttttcgtaag gatcggcgct agtaaatgat actcaaagcc
    10561 actttctgtg atcaaaggag caaaagtttt agaaaagcca aagaatacac agtaataacg
    10621 gtctttcata gctttggcaa cctcaatcat tcgtgttgtc tcagctaaat tgaatactgc
    10681 aggagcaaaa agaattgtct tcatgaaatc ctccttattg gaatgagtct atcccttcgt
    10741 ttcttactag tatactaaaa aacacagcgt tcgagtagcc tatgtatatt ttgaatatta
    10801 aactgtctct cgctgttttc acaacttcta aatttaaatt ttgtaatcag gttgaaactc
    10861 caattttcaa aaaaacacgc aacaattttt tgatcgattt atatgttgtg ccagataaat
    10921 acgagaagtt gtgtcattaa aaataaacat ga
//
LOCUS       DACECY010000078        10512 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.083, whole genome shotgun sequence.
ACCESSION   DACECY010000078 DACECY010000000
VERSION     DACECY010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.083"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(220..972)
                     /gene="xth"
                     /locus_tag="IWO38_002365"
     CDS             complement(220..972)
                     /gene="xth"
                     /locus_tag="IWO38_002365"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ0314419.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="IWO38_002366"
     CDS             1085..2005
                     /locus_tag="IWO38_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0314420.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="IWO38_002367"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="IWO38_002367"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ0314421.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..4789
                     /locus_tag="IWO38_002368"
     CDS             3311..4789
                     /locus_tag="IWO38_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0314422.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            5066..7128
                     /locus_tag="IWO38_002369"
                     /pseudo
     CDS             5066..7128
                     /locus_tag="IWO38_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7211..7780
                     /locus_tag="IWO38_002370"
     CDS             7211..7780
                     /locus_tag="IWO38_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0314423.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7955..8761)
                     /locus_tag="IWO38_002371"
     CDS             complement(7955..8761)
                     /locus_tag="IWO38_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314424.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8899..10413
                     /locus_tag="IWO38_002372"
     CDS             8899..10413
                     /locus_tag="IWO38_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ0314425.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3727 a         1752 c         2071 g         2962 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agatgctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa
     4681 taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa
     4741 taccatcatc agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat
     4801 ggcagctttg tctcacatat ttattagctg atttatgttt ttttcaaaga aaaggtaact
     4861 agatataact agatataact agatatatta cttaacaaaa ttaaaaagta cataagttat
     4921 tattccgatt ttcttgaaac aataaaaaat caaaaaaaat actctcctaa tagtccatct
     4981 tcttttgatt ttatttatat aatgaaatca tcttatgaaa attactactt catgtttaga
     5041 caagagtagg gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac aactaaataa
     5101 acacgcttgt cgtttagata aggaacggtc gtgttctgaa aaacactacg ttaagtttat
     5161 gatgcgcacg atgctgatca gtttatctgt aggtgtctat atgcatgtac ccgttcaggc
     5221 aacgacacaa accatcaagg tcgtacgtac ctctgtttct gagaccactg attcccctat
     5281 cactattact catactttca ctgatgcaaa tggaaaacag ctacttcctg cattgacttc
     5341 ctataataaa gtcagttatc ctccaacgat aaaccactac tggttaattc ctaacaaaac
     5401 attatggaca tcggaaaccg gaagcagtgt gccgctttct atcttttccg atatgactga
     5461 tcctacaaaa ggtgatcagt taaaacagat cactgatatt ttgaatcatg ctttgacaga
     5521 tataaaggac ggtggaagcg cacaagtttc ttatgtatat gaaaaagatt attcacagtt
     5581 tgaaagaaag aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg atctggtaga
     5641 aaggctgaag aacgcagatg gaacacaagg agatatgtcc aaagtaattt caaacaatgg
     5701 gaatatcgtg gatacgacta atacaggaac atctactatt tccttgtcct attttgatcc
     5761 aattgcttta aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg atcaaacagc
     5821 tatcaaagga aaagatcttc acttaagaac aggagagagt tacgccccta aagatcttgt
     5881 tgaatatgta acaaaaagcg atggaacttc aggaaataaa gaagaaatca aaatgaacgg
     5941 gacagtagat actacaactc cgggagaata tcccgttgta ttaacttata tagactcgaa
     6001 aacatcaaat gtggcagaaa caacagctgt agtttatgta gaagcatctg ctgcttcatc
     6061 gactgtcatc actcatttta tagatagtca aacaaataaa gaacttcaag cagaaacaca
     6121 tatgagtagt gtaaatcgta ttcctgattt gcgcctacct gctgaatttt ctgatcgagt
     6181 gccaaatata tagatttatc gacttatgaa caaaatggga aagtcacttc tatttctgat
     6241 tggatgcaag caaacggatt agacagaaaa agtcactggt cggagatcct tgaagtactt
     6301 cactcagaac tgatgaataa taggaagcaa tcaggagggg aaattacctt tacttatgtt
     6361 tatataccag acaatcgttc attagaaggt catgatctta ttgttcatcc aaacgaaaaa
     6421 ataaacaaag aagagttgat ccaatcagca acaaattcct taggtaaacc cgtaccgtta
     6481 aaggatgtat cggttacaat caatgaagta ccattggagg atggttattt gaccaaacaa
     6541 acaggtgatt ttacgattat gtatagttat catgacccgt attcactcca agaactccat
     6601 gctcagtcac tattacatgt tttaccatta aacaaacaga tagatcaaaa agaacaaaaa
     6661 agacaagaag aaagcttgtc agaaaaaaaa caacaaatgg ctcatgcagt ttctccaaca
     6721 gggaaagtaa aacaaacaag aagcctagag ttagcaagaa tatctagaaa aatctcagaa
     6781 tctaataaaa cgataaggca agaagctcat ttggccccac aagtttctgg attggaaaat
     6841 gaaattttag agcctaaaat aaaacaacaa tcagaaaaag aatttgttgc taaacagtcg
     6901 gaagatcaga aggaatcaga tgaccagaag gaaacgaaga aaaaacgctt gtcaactatt
     6961 gattccaccg atgagcatct gcagccgcaa gtaccacttc ctagtccggg aacacccgca
     7021 cctggtggag gtattccttc ttctgcttta ggagatttca tgcgaggtat ggcaggaaca
     7081 tgggtatacg ctaatcgaaa tgaatttgac gcagatgact ggttctgatt tatatctcta
     7141 tctgttatag tcaaataagt agctgagtca tatgttattt ggcataatgg agacgaggag
     7201 ggatagaaaa atgttagata aaacgttacc ttacgcagaa atctggatga caagaccact
     7261 tcaacaaaat gttccaatca cgcctttagc ggaaggtttt gaatttcgat tttatcaaaa
     7321 agaagatgaa tctgcttggg cggagattga gacttccgtt ggggaatttc aaacgacaga
     7381 tgaagcactt gagtattttc aacaaaactt tgctccttat cctgaagaac ttgaaagaag
     7441 gatgtttttt gtagagacaa aaaaaggaga aaaagtagca acctgtaccg cttggcgaaa
     7501 aaaatcaaga gagcaagtgt atccggtttt tcattggtta gcagtgaaac cagattacca
     7561 aggaaaaggg ctcgcaaaag cattgactgc cagagtcttg caagaatttc ctaccctcca
     7621 tacggacgga ccaatctatc ttcacacaca gacatggagc catcaggcga ttgctcttta
     7681 taaaaaaatg ggttttactt ttattgctga aaatctagac ggcacaccta atccagatta
     7741 tgaaaaagtt atgcaggttt taagtaaatt agataaataa cgaaatggtg aaataagtcc
     7801 tcagtattgg gataaaaaac gaaatagtca ttcctcatat cttacttttt tgagttaata
     7861 ctatcggata actaatggca tgccatacat tcaaaaaaga aggggcagtg acaaaagtat
     7921 tgtcactgcc ccttctcttg ttttttagca tgttttatcg tttgattttt ttaggaataa
     7981 aattcttgat gaacgtaaat ttttggtaat cgtttcgact atagaggtaa ccaaatacac
     8041 tgaaaacaac tgctccaata aaattaacaa ataaatcctt catagtatcc atgattccca
     8101 cgtctaagta accattttgg atgattgttt tatctgtttt accatctgca ttttgacttt
     8161 caatgattgt ccgagtgata tctggaacat gatagacagt gtttccgtct ttacttaact
     8221 ccacactatt gatttgctgg atgatcgtat ctttttgcat atcgaattgc agtaatttat
     8281 ctgctgaaaa ttccacaaac tcccacataa caccaactgt catcgaaaaa cagaaggaaa
     8341 caactgctag taataaagga gtcaagttca tggtttttgt atttttattg agcaaataaa
     8401 caagggaaaa gccgacaccc ccagctaaaa atccgctgaa tgcatgtaga aaagtatccc
     8461 acattgaaaa atagccataa aaatcactta actctcctaa aactgttgaa ctaaaaataa
     8521 aagataacaa aatgatttct agcatatttg gaatctgtat ctttaatatc ttttccagaa
     8581 aataagggat ttcaaataaa attaatgtca gtaataaaag cattgtattt ccccagtgtt
     8641 ggttccagac ctgatccact agcataataa cgacgatgat acgtaataaa atatagattc
     8701 cgtttgtgat tctacctgca gtttttgtac tgttcgtaaa aaaggttttc aaatgattca
     8761 taaaataact ccttcatgta tatccttaat tatactaaga agcgtgtgtt taccaaagct
     8821 atatgactta aagaaaaaca gagttgatat tgtgaaaaaa atcacttaat atagtataag
     8881 gagaaaaggg ggatacaaat gaaaaaatta gttgctggtt tattaacagc cgttgcactt
     8941 cttgctatga gcgcatgcgg cgcagatcag aaaaatacag cgcagacaag ttctagtacg
     9001 aaggaatctg ccgaagtatc atcaggcgct tctgaacaag tatatacaga tccaaaagaa
     9061 atgaaagaat cttatgatgt cgtgattatt ggagctggtg gtgcagggat ggcagcagct
     9121 atcgaagcaa aagatgctgg gttgaatcca gtaattttgg aaaagatgcc tgttgccgga
     9181 ggaaacacat ctaaatcatc aagcggtatg aatgcttcag aaacaaaatt tcaaaaagaa
     9241 gaaggaatca aagactcgaa tgatgccttc tatgaagaaa cgctaaaagg tggtggagga
     9301 acgaatgata aagaaatgct tcgatacttt gtcgatcact ctgctgatgc ggtcgattgg
     9361 ctagatcaaa acggtatcaa actggacaac ttaacgatca ctggagggat gagtgaaaag
     9421 aggacacatc gtccgtctga tggctcggct attggtggat acttagtgga ggggttattg
     9481 aataacgtcc atgagcggga aatccctatt tttgtcaacg cagatgtgac agacattaag
     9541 aaaaatgggg aaacggtcaa tgaagtgact gtacatgttc aaggagaaga accaaaagag
     9601 gtcaccggaa aagcaatcgt agttaccaca ggcggttttg gtgccagcaa agaatttatt
     9661 gaaaaatatc gtcccgattt agctgattat attacgacaa atcaagaagg atcaactgga
     9721 gatggtatca ccatgattga aaaattaggt ggacaaaccg ttgatatgga taaaatacaa
     9781 attcatccaa ctgtgcagca ggatgagggg gtcttgattg gtgaggttgt tcgcggagaa
     9841 ggagcaatct tggtcgatca agaggggaaa agatttgtca atgaaatgga tacaagggat
     9901 aaagtatctg cagcaattac agcattgcct gaaaaatcag cttatttgat ttttgaccaa
     9961 ggcgtgcgtg atcgtgccac agctattgaa ttttatgacc aaaaaggata tgtcacaact
    10021 ggaaagacaa tggaagaatt agcagaaaaa atcgatttac caaaagaaac actagcaaaa
    10081 acagttgatg gatggaatac ggatgttgcg gagaaaaaag atgctcaatt caaccgtgcc
    10141 acagccatgg aacatgactt atcgtctccg aattattatg cgatcaaaat cgcaccagga
    10201 atccattaca cgatgggcgg tgtgaaaatc aatacgaaca ctgaagtatt agataaagaa
    10261 aaccagccaa ttagggggtt atatgctgct ggtgaagtca caggtggctt acatggaaac
    10321 aaccggatcg gtggaaattc agttgcggag atcattattt ttgggcgaca agcgggcata
    10381 caagcagctc gatttgcttc ggaaaatgaa tgaaatagaa cgtaaataaa aagaggttgt
    10441 gaaatcagcg ttttgagcta ataccgaggg tcagctgatt gaacactgtt tagtgaagag
    10501 gttgtgaaaa ag
//
LOCUS       DACECY010000079        10496 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.084, whole genome shotgun sequence.
ACCESSION   DACECY010000079 DACECY010000000
VERSION     DACECY010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.084"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..360)
                     /locus_tag="IWO38_002373"
     CDS             complement(<1..360)
                     /locus_tag="IWO38_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ0314426.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLGI"
     gene            complement(712..1071)
                     /gene="rplT"
                     /locus_tag="IWO38_002374"
     CDS             complement(712..1071)
                     /gene="rplT"
                     /locus_tag="IWO38_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ0314427.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(1124..1324)
                     /gene="rpmI"
                     /locus_tag="IWO38_002375"
     CDS             complement(1124..1324)
                     /gene="rpmI"
                     /locus_tag="IWO38_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ0314428.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(1396..1920)
                     /gene="infC"
                     /locus_tag="IWO38_002376"
     CDS             complement(1396..1920)
                     /gene="infC"
                     /locus_tag="IWO38_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ0314429.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            2240..2872
                     /locus_tag="IWO38_002377"
     CDS             2240..2872
                     /locus_tag="IWO38_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ0314430.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2874..3779)
                     /locus_tag="IWO38_002378"
     CDS             complement(2874..3779)
                     /locus_tag="IWO38_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0314431.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(3864..4517)
                     /locus_tag="IWO38_002379"
     CDS             complement(3864..4517)
                     /locus_tag="IWO38_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0314432.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVCV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVSI"
     gene            4839..5783
                     /gene="mvk"
                     /locus_tag="IWO38_002380"
     CDS             4839..5783
                     /gene="mvk"
                     /locus_tag="IWO38_002380"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ0314433.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5776..6753
                     /gene="mvaD"
                     /locus_tag="IWO38_002381"
     CDS             5776..6753
                     /gene="mvaD"
                     /locus_tag="IWO38_002381"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ0314434.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6766..7851
                     /locus_tag="IWO38_002382"
     CDS             6766..7851
                     /locus_tag="IWO38_002382"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ0314435.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7848..8912
                     /locus_tag="IWO38_002383"
     CDS             7848..8912
                     /locus_tag="IWO38_002383"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ0314436.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(9161..9964)
                     /locus_tag="IWO38_002384"
     CDS             complement(9161..9964)
                     /locus_tag="IWO38_002384"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ0314437.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9984..10289)
                     /gene="kduI"
                     /locus_tag="IWO38_002385"
                     /pseudo
     CDS             complement(9984..10289)
                     /gene="kduI"
                     /locus_tag="IWO38_002385"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            complement(10271..>10496)
                     /locus_tag="IWO38_002386"
     CDS             complement(10271..>10496)
                     /locus_tag="IWO38_002386"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ0314438.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
BASE COUNT         3106 a         2220 c         2008 g         3162 t
ORIGIN      
        1 aatacctaat gacatcaaga tcggcaacac aataactgca atagaaatca ctggtccgat
       61 tcctgtcatc gacatgaata taacagcggt tacaatacat agtaagctca tcgtgattct
      121 tggcttgtcc cctccaagtt caactacctt tcgaatcagg gtagctgcga ttccgctatc
      181 tactagaaca cgcccgaaaa aggcaccaaa gaagacattt accaagatcg atttggcgta
      241 ctcagctggc cccgtcgtat agatatgcgt caacacatca attactcctt gcccttccat
      301 cgccgagttt ggtgaaaacg tgtttccgac tagcgctaat cccatccata cagtagccat
      361 gatgacaaaa gccgaccatc aagttgtagc cttttacaca gtaccagacc atgaaaaaga
      421 atgagataac taacaaaata ccaataataa tatcactcat gttattcctc cgtccatttt
      481 gtttttctga taagcaaact aatatatcag tcaaactcat tctaacttat gtagttagcg
      541 ctttcattaa aggatgacca taaatgttca gttttcaatt aaaaaacctt aattatgtta
      601 ttttagactt atacgttatt gtcctgtttc atttgatgga ggattgtaaa taaaaaaaga
      661 ggaaagaaca taacttttcg ttatcttctt tcctctttat aattgtttag cttatttagc
      721 caaagcttct tttgcttggt cagccaatgc agtaaatgct gctgcatcgt taacagccaa
      781 gtcagctaac attttacggt tgatgtcgat ttcagcaact ttcaaaccgt gcattaattt
      841 tgagtagctc aagccattca tacgagctgc tgcgttgata cgagcaatcc acaatttacg
      901 gaagtcgcgt ttcttttgac gacgatcgcg atatgcgtag ttgtatgaat tcattacttg
      961 ttcttttgct gttttaaata aagtgtgttt tgaaccgtag taaccttttg ctaattttag
     1021 cactctttta cgacgtttac gagttactgt tccaccttta acacgtgcca tgtataattc
     1081 ctcctaataa aaataaatct tcagattctg ttatttgtac ttcttatttc atttttgcta
     1141 gttgttggcg aatacgtttg aagtcgccac tagaaaccat gcttgcttta cgcaattgac
     1201 ggcgttgttt ttttgttttg ccgtggaaac ggtgacttgt aaacgcgcgg aagcgtttca
     1261 atccgccttt accagtacgt ttgaaacgtt ttgctgatcc gcggtgtgtt ttttgttttg
     1321 gcatgactat atttcctcct caaaatcttt cgttatccga ctatcggctg tccaacagcc
     1381 agttagtcat tcaaattact tactgtcgtt tttcggtgcc agcgtcaaga acatgctgcg
     1441 tccgtccatt ttcgcttttt gttccactgt tgcaatatcc gcagtttctt cagctaagcg
     1501 attcaagact ttttgaccaa tttctttatg ggtaatggca cggcctttga aacggataga
     1561 agctttcact ttatcgccct tttccaagaa cttacgtgca ttacgaagtt ttgtattgaa
     1621 gtcattcaag tcgatcgtag gacttaagcg aacttctttt acattgatca ctttttgttt
     1681 tttacgcgct tcgcggtctt tcttttgttg ttcgaaacgg tatttaccgt agtccatgat
     1741 tcgcgcaact ggtggtttcg caccaggggc aactaacaca agatccaagt ttgcttgttc
     1801 tgcaatattc aatgcttctg cttttgtctt aactcctaat tgctcaccat ctgaaccgat
     1861 caatcgtaac tctcgtgcac gaatgccgtc gttaaccatc atatcctttg ctatggtcat
     1921 tcacctccaa atattatgag agaaagaaaa gcttaccgaa taaacggcaa taaaaaaacg
     1981 agttctctta cagaacccgc gcgtacttca cctaatagaa ccagtgaaaa ctatctagcc
     2041 cagtgacatt atcaactaag gcgagaagcg ggtgcttctg cttttatttc tcaaccatta
     2101 tagtatagca aaaactaatc tttttgtcaa tgtttttcat acagtttgtc ttccttttct
     2161 gaaaatgatt agattagcag gctttctagc agtgattccc gcttctttgc tatactaaag
     2221 aaaaacgagg tgaacccaca tgaaaaaaat cgaacctatt ccaccttctc ttcctcttct
     2281 tacaaaggga gagttttatt tagaagatga ggagatcttc tctggtcttc tgatcaacgg
     2341 aggtaaccaa agctatctcg atgccaaaaa tatggtatta agagagagtc atatccaaaa
     2401 acttacgatg caaaaaacag gtttacgtca ttttgaatgc agtaatgtca tttttgaaaa
     2461 atgtgatttt tccaatctgg agtggattgg tggcagtttc caccaagtcg tgtttcatca
     2521 atgtaaatta acaggtacga attttgccga aagttatctt cgagactgta ctttcactga
     2581 ttgtattgct tcgatggctt cctttagtag tacaaacttg aaagctgtct ccttcgatca
     2641 ttgtcaatta gaagacagcg aattttatga ggtcacttgg aaaaaccttt ttttgtctga
     2701 caatcagtta accggttcga actggtttcg aacttctctg aagggattag actttacgac
     2761 taatttcttc acaacaatcg ctttatctca agattctttg gcgggactaa tcgtcaatca
     2821 agagcaagca cttgtgatcg cccaagccct tggtttgcag atcaaagact agatcaacga
     2881 atccgccgcc acagtgcaat aagcatccaa atagataaga ccaaactgat gatcaaggcg
     2941 atttcccatc cgaaaacgga atgggtaaag ggtaactgga gattcattcc gaagaatcct
     3001 gtcacaatag ttggaacggt caagatcagc gaccagaccg tcaaaaattt catcgtgtca
     3061 ttcaagttgt tattcagcaa attattataa gtgccagaga gctggtctaa aatctgcgaa
     3121 gctaagttcg tcatttcaac tgcttgtcgt gcttcgatca gtgcatcgtc caactcttct
     3181 ttttcctttt ctgacaactt gcgataaata gccagtgcct tgatttgttc taaaagtact
     3241 acattttgtt ttgttcctgt aactaagtaa accaaaccga tctctaaatc tgaaagttgc
     3301 agcagattct tgttcgttgt tttttctctt aatttcagat taagccgctg acgttcacta
     3361 ttcacttcct cgattaaagg aaaaaaggaa tcagaaatca aaaacaatgt acgaaacaat
     3421 aaactgtata cactcatgtc tggcatccgt tccaaaagcg atcgaattag tcgaatcacg
     3481 tactcggtat tcggcgtggt aaatgtaaac aaaccgtctt cttttaaaat aaaagcgata
     3541 ggactagttt cataatgatt atcgactttt tgctggtgcg gaacatttga tacaagcagt
     3601 aacgcttcat caaaagaatc gtactctacg cgcgcccgtt catttttatc tagagaataa
     3661 gtcagcatct cgtcactaat gccatattct tcttttaagt ttttaacagc tagcacatcg
     3721 ttttgctgta cattgatcca aaaattacgg tcttgattaa aatagtgttt ttcccgcata
     3781 ctctatcctc ctgacatttg tttccagtat aaagaaaaaa gtcgctcaaa taaaggttta
     3841 ccctttattt gagcaactgt aaatcaaatg gatactttgg tttggcgaat ctctgctaat
     3901 ttttcttcga tcattttcaa gacttcttct tttgcttgag gatcttccac aaaattatag
     3961 cggtctccat cgatttggat ttttggactt tctttgtatt cttcatacca ttgatcataa
     4021 cgtctgttca attctttgta ataatcgtat agacttgggt caaaggacaa ttgttcatac
     4081 gaacgaccac gtttttcgat acgctctaac atcgtatcaa aagatacacg aatatgaact
     4141 aataaatccg gatgtttttt atgggcagca tagggaagtt cttccatcat gttttccagc
     4201 agttcatcat acacacaaac ttctgtttct gttgctcttc ctagatctgc atttagatgg
     4261 aaaagcagtg aatcttcata aatcgaacgg tccaatacat tattgtcatc tttcattgct
     4321 tgtttgatgc ttgcaaaacg tttattcaaa aaataaattt gcagcaaaaa agcatactgt
     4381 tcagggtttg cataaaataa gggcaagact tcattatcat caatcgattc ataaaatgcc
     4441 tgactatcaa agtgttcagc catcatttct gttaaacttg attttccggc accaattgta
     4501 cctgctaaaa cgatcacgct catttacctc cagctcactt catcactaaa tattgtgttt
     4561 aactccaacc ttttttaaat atacccccaa tatctagtag tgtcaattgt attttttcta
     4621 gtcaaagaag aaaaaaagtc aaaaaattct taagaaaaaa gtgttttttc tttagaaaac
     4681 gtattcttag ttttacttct gagatggttt tagtaaaata aaggaggaat gttttttgag
     4741 acccttagtg gacaaaaaaa ttcgtgaagc agcgcaactc gttttgggcg ctttattttt
     4801 tgcttaaaaa tcacttttac gtgagaagga gttagattat ggcaaactat ggccaaggag
     4861 aatcaagcgg aaagatcata ttgatgggcg agcacgcggt tgtttatgga gaaccagcga
     4921 ttgcctttcc tttctatgca acaaaagtca ccgcattcct tgaagagctg gatgcaatgg
     4981 acgatcaact ggtttcttcc tactattcag gaaatttagc cgaagctcct catgcattaa
     5041 aaaatatcaa aaaattattc attcacttaa aaaaacagca tgacatccaa aaaaacttgc
     5101 aactgaccat tgaaagcacg attcctgctg aacgtggaat gggatcaagc gctgcagtcg
     5161 ccacagcagt cactcgtgct ttttatgatt acttagcatt tcctttgtct cgtgaaatac
     5221 tattagaaaa tgtccagctt tcggaaaaaa tcgcccacgg taatcctagt ggaatcgatg
     5281 cagccgctac tagcagcttg cagccgattt attttacaaa agggcatcct tttgactact
     5341 tttctttgaa catcgatgct tttttgattg tcgctgatac aggaatcaaa ggacaaacaa
     5401 gagaagccgt caaagatgtc gctcacctct ttgaaaccca gcctcatgaa actggacaaa
     5461 tgatccaaaa attaggatac ttgacgaagc aagcaaaaca agcaatcatt gagaattcac
     5521 cagaaacgtt ggcacagaca atggatgaat cacagtcact tctggaaaag ctgacaatca
     5581 gcaatgattt tcttaatcta ttgatccaaa cagcaaaaga taccggggca ttgggtgcca
     5641 aattaactgg cggtggacgc ggtggctgta tgattgcatt agcacaaaca aaaacaaaag
     5701 cccaagaaat cagtcaagca cttgaagatg cgggtgctgc tgaaacttgg atacaaggat
     5761 taggagtaca tacctatgtt taaaggcaaa gcacgcgcat ataccaacat tgctctaatc
     5821 aaatattggg gtaagaaaaa tgaagaactc atccttccaa tgaacaacag cctttcgtta
     5881 acattggatg cattttatac agagacagaa gtcatctttt ctgatagcta tatggtagat
     5941 gaattttatc tagatggcac cttacaagac gaaaaagcaa caaaaaaagt cagtcagttt
     6001 cttgaccttt ttcgtaaaga agccggcctc tcactaaaag cttcagtaat cagtcagaat
     6061 ttcgttccta ccgcagctgg attagcctcc tctgccagcg ggctagctgc tttagcagga
     6121 gcttgcaata ctgctcttaa gcttggatta gacgatctct ctctttcaag atttgctcga
     6181 cgcgggtctg gttcagcttg ccgaagtatt ttcggtggtt tcgttgaatg ggaaaaaggc
     6241 catgacgact taagttctta cgctaagcca gtcccttccg attctttcga agacgattta
     6301 gcaatggttt tcgttttgat caacgaccag aaaaaagaag tgtccagcag aaatgggatg
     6361 cgtcgaacag tcgaaacatc cagtttttat caaggctggt tagattccgt tgaaggggat
     6421 ctatatcaat tgaaacaagc aatcaaaaca aaagatttcc aacttctcgg agaaacgatg
     6481 gaaagaaacg gactaaaaat gcatggaacg actttagctg cccagcctcc attcacttac
     6541 tggtctcctg acagcttaaa agcgatggat gcggtgcggc aattgagaaa aaaaggcatt
     6601 ccatgctact ttacgatgga tgctggccct aatgtcaaag ttttagtgga aaacagccat
     6661 ttatctgagg tacaagaaac atttaccaaa ttatttagca aagaacaagt aattactgcc
     6721 catgctggtc caggaattgc gattattgaa taaagggaga tttttatgat tgaagtatct
     6781 gcaccaggca aactctatat tgccggagaa tatgcagttg ttgaaacagg acatccagca
     6841 gttatcgctg cagtcgatca attcgtaaca gtcactgtag aatccgcacg aaaagtcgga
     6901 agtatccaat ctgcacaata tagtgggatg cctgtacgtt ggacaagacg caacggagaa
     6961 ttagttttag atattcggga aaatcctttt cattatatcc ttgctgctat tcgtttgact
     7021 gaaaagtatg cacaagaaaa aaacattctt ttatcctttt atgatctgaa agtaacaagt
     7081 gagttagaca gttcaaatgg ccggaaatat ggattgggtt ctagcggtgc cgtgaccgtt
     7141 gcaacggtca aagccttgaa tgttttttac gcgttgaatt tatctcaatt ggagattttc
     7201 aagattgcag cactagccaa tttagcagtt caagataatg gttcctgcgg cgacatcgct
     7261 gctagctgtt atggtggctg gatcgctttc tcaaccttcg accatccttg gctccaagaa
     7321 caagaaactc agcattctat cagtgagtta cttgccctgg attggccagg tctatccatt
     7381 gagccattga ttgctcctga agatttacgt ttattgattg gttggacggg tagccctgcc
     7441 tctacttctg atttggtcga tcaagttcac cgttcgagag aagacaaaat ggtggcttat
     7501 cagcttttct taaaaaacag tacagaatgt gtcaacgaaa tgatcaaagg gtttaaagaa
     7561 aataatgtaa cgttgatcca acagatgatt cgaaaaaacc gacaattact gcatgattta
     7621 tctgcaatca ctggggtcgt catcgaaacg cctgctttga acaaattgtg taatttagct
     7681 gaacagtatg aaggagccgc aaaatcttct ggtgcaggtg gtggcgattg cggaatcgta
     7741 attgttgacc agaaatctgg catccttcct ttaatgagtg catgggaaaa agcagaaatc
     7801 actccactgc cgttacatgt ctatagcgat caaagaaagg aaaaccgatg aatcgaaaag
     7861 acgaacatgt ttcactagct aaagcctttc atgataaaca aaaaaacgaa tttgacttcg
     7921 tacggatcat ccataaccct ttgccgcaaa tagcagtttc tgatgtcgat ctaagtacac
     7981 aggcagttgg gtttacacta agcagcccat tttatatcaa tgcgatgact ggcggaagcg
     8041 aaaaaacaaa aaaaatcaac caagatcttg caatcgtcgc aagagaagca gatttgatga
     8101 ttgccactgg ttcagtcagc gctgccttga aagacccttc tttagccgat acttatacga
     8161 ttatgcgaca agaatatcct cacgggaaaa tcattgccaa catcggtgca gggacttctg
     8221 tcgaaagagc ccaagaagct atccgattgt ttcacgcaga tgccttacag attcatttga
     8281 atgcacctca agagttggtt atgcctgagg gagaccgtga ttttaccaat tggaaagtac
     8341 ttattcaaga gactcaaaca gccattgacg tccccctcat cgtaaaagaa gtcggttttg
     8401 gcatgacaag agaaacgctc aatgatctag ctgctttagg agttcataca gttgatatca
     8461 gcggtcgaag cggaacaagc ttcacccaga tcgaaaacgc ccgtcgctcc aagcgggaac
     8521 tgagttattt agctgactgg gggcaatcga cagtctcttc attgcttgaa gcaaatgaag
     8581 cagacacttc tatggaaatc ctagcctccg gaggtatccg caatgcctat gacatattta
     8641 aagccctttg tctcggcgca aatgcagtgg gtacttcagg aaccgtactg acgcatctga
     8701 tgaatcacgg cgtagaagaa actattatac tgatgaagca atggcaagaa gagcttcgtc
     8761 tgctttatac tatggtcggc gcaacgaata ccgcggccct acaccaacaa tcactgatct
     8821 tttccggacc agtgaaggat tggtgcgaag caagagggat cgacttagtg aagtatggaa
     8881 gacgcacaga aaagagcgtg ggacaaaagt aaagaacact tttatcccac gctctaaaca
     8941 cgtataaacg gcggaagcaa aagcaactcc ttcggaaata agctgaaatt cacaaaaatt
     9001 tgaaaagcaa ttttcgtgaa ttccttctta tttcttggag ttaaacgctt ctgtcccagc
     9061 ctcttttttc gaataaatga tgtttaatca gctgtccttc ggtattcgtt caagccgctg
     9121 atttcacaac ctctccacta aacggtgtac taaagctctt ttaaggttgt tttccgatat
     9181 aggcaagtat gcctccgtcc acatacaaga catggccgtt aacgaaatca gatgcgctag
     9241 aagccaagaa gacagatggt ccagccaaat cgattggctc gccccagcgt gctgctggcg
     9301 tacgtccaac gataaattga tcgaatggat gacgttcacc gttttcttgt gtttcgcgta
     9361 atggagcagt ttgcggtgta gcaatataac ctggtccaat tccattacat tggatattgt
     9421 attgaccgta ttcagaagcg atgtttttcg tcagcatttt taatccgcct tttgccgcag
     9481 cataagcact gaccgtttcg cgtcctaatt cactcatcat ggagcagata ttgatgattt
     9541 taccgccgcc tttttcgatc atatcccgaa taactgcttt tgaaacgatg aatggggcat
     9601 ttaaatccac gtcgattact tggcggaagt ctgccgcatc catttcgatc atcggtgtcc
     9661 gtttgatgat ccctgcattg ttgactagaa tatcaactga acctacatct tctttgatct
     9721 gttgaaccat cgcttgaacc gcttcttcat cggtcacatc gcagacataa ccttttgcat
     9781 cgatgcctgc ttctttataa tgcgctatac cttcatcaac tgattcttga ttcaagttat
     9841 taaatacgat ttttgctcct gctgtagcta aagagcgtgc aatctcaaat ccaataccat
     9901 agacagcacc ggtgactaat gctacttttc catctaaacg aaacatatcc atactaaatt
     9961 ccatcatttt ctctccctta tgcttatttt aattgatcca tttgcaccat atccatgtct
    10021 gtataggtga tattttctcc gcacattgcc cagatgaatg agtaattgct tgtacctaca
    10081 cctgaatgga tcgaccagct tggcgaaata atggcttgtt cattgtctac gactaaatgt
    10141 tttgtttcat ctggtttgcc catcatatgg aacacacgtg tatcttcatt ggcataatca
    10201 aaatagacat aagcttccat acgacgttca tgtgtatgac aaggcatcgt attccaagca
    10261 cttcctggtt ctaaaatcgt ataacccatt tataaatgac agctatcgct gaagcttcta
    10321 ctgctgtaaa tatccccgtc aagataccac cgataatgat gacaatcaat agaaggctag
    10381 gaatcgcctc ttttattatt tttccagcat gagccatctg tcctttttcg acaacagggt
    10441 atttattctt tttagcaacg atgaatgcta ctagcatgat tcctagcgcc cacaaa
//
LOCUS       DACECY010000080        10411 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.085, whole genome shotgun sequence.
ACCESSION   DACECY010000080 DACECY010000000
VERSION     DACECY010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.085"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            111..851
                     /locus_tag="IWO38_002387"
     CDS             111..851
                     /locus_tag="IWO38_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314439.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(1142..1810)
                     /locus_tag="IWO38_002388"
     CDS             complement(1142..1810)
                     /locus_tag="IWO38_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0314440.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            1944..2231
                     /locus_tag="IWO38_002389"
     CDS             1944..2231
                     /locus_tag="IWO38_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314441.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            2647..4185
                     /locus_tag="IWO38_002390"
     CDS             2647..4185
                     /locus_tag="IWO38_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ0314442.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            4182..5147
                     /locus_tag="IWO38_002391"
     CDS             4182..5147
                     /locus_tag="IWO38_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0314443.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            5134..6456
                     /locus_tag="IWO38_002392"
     CDS             5134..6456
                     /locus_tag="IWO38_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0314444.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      6519..6623
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6697..7440
                     /locus_tag="IWO38_002393"
     CDS             6697..7440
                     /locus_tag="IWO38_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0314445.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            7455..8447
                     /locus_tag="IWO38_002394"
     CDS             7455..8447
                     /locus_tag="IWO38_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0314446.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            8459..9196
                     /locus_tag="IWO38_002395"
     CDS             8459..9196
                     /locus_tag="IWO38_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0314447.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            9186..9908
                     /locus_tag="IWO38_002396"
     CDS             9186..9908
                     /locus_tag="IWO38_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ0314448.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(9992..>10411)
                     /locus_tag="IWO38_002397"
     CDS             complement(9992..>10411)
                     /locus_tag="IWO38_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ0314449.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3456 a         1792 c         2172 g         2991 t
ORIGIN      
        1 atttatgaaa ttcatcgcta ttttcttaaa ataatcagcg cattgctact gaaaacaatg
       61 aatctgtgct attattgaga aaagttccat ttttgagaag gatggtttga atgaaagaaa
      121 tcagacgaaa acatttccgt tttcctgcgt acgatgatga gttaggcgta aaattaacca
      181 acgacaagaa gcgagtatta tttgacggac aagaagatct tttgactgaa cagaatcagt
      241 ccccgtttga acagatgaaa gaatttcatt tcacgaacac gaacaccgag aaaatccgtc
      301 gcaagcgtcg tgcaacaaat ggattgaagt cagctccgcg taaggaaaat ttggaaaagc
      361 ataaaggaaa attaccggat tataccggcc attaccaatc agaaatcaca tcgacaggac
      421 gaaaaaaact ttttggtgat tcctctcgtt ctgtcagaca aagtgaaaca aggacagagt
      481 ccaagcatac tacacgaaac gcatctgttg acacgaaggt gcgggagcgt tcttattttg
      541 ttcctaaata tgtacccgca tcgattatcc cagaccctag agaaagcaaa atttctgagg
      601 atcatttaat cgaatcgatg aaaaaaacgg atgactctta tctgttgttc gatacagaac
      661 cgacgccgta tcaagaaaaa agaaatggtg agccgtctgt acgaacattc aatcgaacaa
      721 aaacgttaga ggaaacaaag cagtcagaag aaaaaaaatc gaaaaaaagc catggtatct
      781 tagaacgctc attacaagga ttgatcgatg atcaaggaaa ctctttggga gaaaatagct
      841 attttaaata agtgatatac aaatttatat tcggctacag ttggaaagga aacgctgcgg
      901 cgtttcctta ttttatactc tatccgaaag tttatatcgt atcctcaaat ttttttgagt
      961 ttaagcgaat tttttctccc aataagtaag aaaataatgg caaaataaaa aaacaaacta
     1021 ttttcgcaga tcgtttgcaa aaatagttta tttttttata aagaagattt tactgaataa
     1081 tatcatttta gtttacataa tgatatttct gtaaattaaa taaagtagtg aaaaataaga
     1141 atcagtgctc taactgttca agaggatcaa gtggttcaaa tgtttctgta gtctgttttg
     1201 cttccaattg gggcaaaaca gatccaattt ccagatactt cactttattc aatgtataag
     1261 atagaataga acagctaagt aatgtataca gaaaatgtgt gagatccaaa taaagcaagg
     1321 agacaactaa aataccaaaa gtaatcaaga aaagactttt gccaatctta atatgaaaat
     1381 atttttcaat ggcttgataa atgatatctg ttccgcctgt gcttccgccg aatcgaaaga
     1441 cgagaccgca accaatgcca gaaagaatac ctgtcatcaa actattgatc agtagactat
     1501 ctacagcaaa atgtatatga aggctctccc ataaatgtaa aaagaaaact agcgcaaatg
     1561 ttccgacgat cgtccggaga aaagcgtgtt ttccaaaaag aaacaatgag ataagcaata
     1621 aaggtaaatt aaaaagcagt gaagtgacag aaggagcgaa tccaaaaaga ttatgcaaaa
     1681 gcaaagcaat accagtaacc ccaccatctg ccagcttaga tggaatattg attcctgtca
     1741 cagctaatcc gtagattcct gcgcctaaaa tgattccgca caattctgta aataaattta
     1801 attttttcat ttctagcctc ttttcttttg aaaattttca gcaacttctt tattatactg
     1861 acttatactc ttacttcaag aggaaaatga aaaaaaatta tttttggctg tttcttttta
     1921 tttatcaaat aaggagtgga aaaatgcgag cagcagactt attgacaatt cttacaaaat
     1981 cacccaaaaa aacagagtat caaggagttt ttttggctca agaacaaaaa ctggtgcctg
     2041 tgacaatggt ccagataaat aaagcaaatc aatttatctt tttatttgaa cacggcaaag
     2101 caccacttgc tatgaaagac atctttcttt ttttgatgaa gaaccgtgag aaagctgttt
     2161 attatcagtc aaaagaagaa ctcattcctc tttatggagt gaaagagata gaaaataaag
     2221 tggtgatata agtaaaaata agaaaacaag acgtttatga tgtcaaaaca accatttttt
     2281 acatttttgg caaaaaagtg tcatgataca gtataatatt cgttgaacag gccaataaag
     2341 gtgttctccc ccacgagatt tctactttat tggctgtttc tttttattaa aataaaagtt
     2401 gtgtgtgtaa aaaagatata gctgattcca agtgacaatt tcgtatagat ttttatggta
     2461 ttcgtagtga aaaaacgaga accaaaaagt tgatccgact ttgttgcttg gattcttttt
     2521 ttgtcagcat atttagaggt atttttttct tttttttgtt cgtgctgtaa ggttaaggca
     2581 agacaagtta gttgggaaaa aggaaagaaa tgtttatact taaagaataa cagaaaggaa
     2641 ggtcgcatga ataattttat acaaacatta agtgaaagaa aaggcgagtt gctggaagct
     2701 gcatttcaac atctgtccat ttctctcatt tctttgctga ttgcagcact gattgctatt
     2761 cctttagcga tttgggcagc caatcataaa aaaatggcag aagtcttatt gcagatcacg
     2821 ggtgtgctcc aaacgattcc ttcacttgcc ttattaggtt tacttatccc atttgtaggg
     2881 atcggaactg ttccagcatt gattgcactt gttatttacg ctttgcttcc gattttccaa
     2941 aacacgtata ttggtctagc tgaaattgac ccgtctattg aagaagcagc ggtagcattt
     3001 ggtatgtcca gaatgagacg cttgctgaaa gtagagcttc ccatcgcatt acctgtcatt
     3061 atctcaggta tccgaactgc cttagtttta atcatcggta cggctacttt ggcagcattg
     3121 atcggagcag gtggattagg gacatttatt ttgttaggta ttgaccgaaa tacacctgtt
     3181 ttgacattga ttggtgcgat cagttctgcc ttgcttgcaa ttatattcag cggactgatt
     3241 cgctggttgc agcataagaa acctcgatat tccatcatta ctttagcagc agtggtgtgt
     3301 ttatttggcg gcgtttcttt atatcagtct gccttgttta aaaatgaaac catcacgatt
     3361 gctggaaaat taggagcgga acctgaaatc ctgatccaaa tgtataaaga attgattgaa
     3421 gacaacacaa aaacagaagt tgaattaaaa ccgaattttg gcaagacgag ttttttgttc
     3481 aatgcactgg atcaaggtca aatcgatatc tatcctgagt tcacaggtac tgtcctagaa
     3541 agcttggtga aggtacctga agcaatgaaa agtgctgatt tgacaaaaga aaaaacatat
     3601 gacgaagcag aagaattatt gaataagcaa ttcgatatga cattattaaa accaatggct
     3661 taccaaaata cgtatgcctt ggcagtcaag agatcttttg cgaacgaaca tcaacttaaa
     3721 aagatttccg atttggcagc tatctctgat cagatcaaag cgggattcac actggaattt
     3781 attgatcgtt cagatggata taaaggaatc caagaagcat atggcttgaa tttttcaagt
     3841 gtacaaagca tggagccaag tttgcgttac caagcaatca ataatggaga tgtcaatatt
     3901 gtggatgcct attccacaga tagccagctc aaggagtacg atctagtgac tctagaagat
     3961 gatcgagggt tattcccagc ttatcaaggc gcaccgctga tgaaaaaaga ttttgctaaa
     4021 gaacatcctg aaatcgttaa agcactgaat aaattaagcg gaaaaataac agaggatcaa
     4081 atgattgata tgaattacca agtcaatgtc gaaaagaaaa agccgacaga agtagcacga
     4141 atcttcctga aaaatgaagg attagtaaag gaggaagatc agtgactaaa ttaatcgagt
     4201 tcaaacatgt gcaaaaaaag tatgatggaa agtatgtcat cgatgaccta aatctggcaa
     4261 tcaaaaaagg agaaatcttt gttttggttg gtccttccgg tagtggaaaa acaactactt
     4321 tgaaaatgat caatggatta tctaaaccat cagcaggaga catctacttc aaaggaaaat
     4381 cgttaaatga atataatctg cagaaaatgc ggtggaatat gggatatgtg ctgcagcaaa
     4441 tcgctttgtt cccgaccatg acagtcaagc aaaatattga agtcatccct gaaatgttag
     4501 gttgggaaaa gcagaaaaga gcagaccgtg tagatgaact tcttcaaaaa gttggcttat
     4561 ctcctgatat ttatcgagat cgtatgccta gagagttgtc tggaggagaa cagcaacgga
     4621 tcgggatcat ccgagctatt gctgcaagcc cagatgtgat tttgatggac gagccattta
     4681 gcgcattaga tccgatttca agaaactcat tacaagaatt ggttttgtcc ttacacgaag
     4741 aattgggcac gaccatcgtt ttcgttacgc acaatatgga agaagcaatc aagctaggtg
     4801 atcgtattgc atttatgaag gatggagaaa ttatccaatg tgacacacca gagcagttat
     4861 tgatgaatcc taaaaacgac tacgttcggc atttctttga tgaaccgaaa cagacgaaag
     4921 agtggcgagt agaagattta gtcgtcaatg gatatttctt aaatgaaata ccagaaaatg
     4981 ctcgtcaaca agtttgcttt gatacgccta tgaaagaagt atattcgctt ctttcggtgt
     5041 atccaagcat tgtgatagta gaaaagcaaa gaagtattgg gtcgctgaca agtaaagaaa
     5101 tctttgcttt tttaagtcga gaggaggaag gaaatgaaaa catatgatgc aatcatcatc
     5161 ggttcaggtg tgagcggctt aagtgctgct tatggattga aagaagcagg taaaacagta
     5221 ctagtggtcg aagaagatct atggggcggg acttgtccta accgtggatg tgatcctaaa
     5281 aaagtcttgt taagtgcagt agaggcacgt aatcgggtaa aacaattgag tggaaaaggt
     5341 tttaacgaga tcccaactgc caactgggaa gaacttcaaa aattcaaacg aacatttacg
     5401 gatccagtcc cagaaagccg aaaaaaacaa ttagcagaag ctgaaatcga tcatctttca
     5461 ggaaccgctc gttttttgga tgattcatct attgaagtca acgaagaagt cttccatgcg
     5521 gattacttag ttttggcaac cgggcaaaga cctacgatct taccagtgga gggaaaggaa
     5581 tacttgaaaa ctagtgcaga ctttttatca ttgcctgtac tgcctaaaga aatcattttt
     5641 atcggtggtg gttatattgc ttttgagcta gctacgattg ccaatgctgc agggagcaaa
     5701 gtaaccattg tccatcataa tcaacggcca ttaaaagaat ttgaggcgtc tttggtagag
     5761 gaagccgtcc atcaaatgga agcatctggc attcagtttg cttttggtgt tgaaacccaa
     5821 aagatcattt cagaaggtac tcgctatcgt ttagtcggaa aagagacgga attagtagca
     5881 gatatgattt tttgtgcgac tgggagacag ccgaatacag aatctttagc acttgagcaa
     5941 gcgaatatcg tctttgataa acacggaatt gcagtcaatg attatctgca aacgagcaat
     6001 ccaaaaattt ttgcgtgtgg agatattgtt tcaaggaaaa ccccaaaatt gacaccagtc
     6061 gccacttttg aaggaaatta tgtagctaag cggataacgg atgcaacaag cgaacctatc
     6121 aaatatccga ttatacctac gatcgtctac gccagtccta aacttgctga agtaggagtt
     6181 acaaaaagtc acgcctcttc gtcagatcaa gtggtagaga tggatcttac cagttggttc
     6241 acctatcatc gtgtcaatga accagttgct aaggctgaat tgacctttga ccagcaaaat
     6301 tatttgattg gtgctgcagt gattagcgag caagcagacg aattgatcga tgatttgaca
     6361 ctcgtcatca atcaaaaatt gactaaaaaa gagttggata gctatatcat ggggtatccg
     6421 acattggcaa gtgatctctc ctatttattg aaataatcag catgataact aaagcgcttg
     6481 ttgctttttc cttcttgtgc tacactttaa aaaatgaata cctgtttctg gggatgtcgt
     6541 agacgacatg agagtaagtt gtgaacttag acccattgaa cctgtcagtt aatactggtg
     6601 aagggagaaa catagcagac gcttgaagcg ggttactatg tcgtgcccat ttcaagcgtt
     6661 ttttgttatt catattagaa gaaaggaggt aacaaaatga agaaaaatct ttatccagca
     6721 gtcagtatgg cattcatttt aattatttgg cagattgttg tcatagtttg gaaaatacct
     6781 gcctttattt tacccggacc tgctgctgta ttttctgcat tagcggcaga tgcacaagta
     6841 ttactttccc attctgtcac gacattaaat gaagcagtgc ttggtttggt aatagctagc
     6901 gttctttctt ttattactgc cttggcaatg gattattgga agtttttaga aatgtctttt
     6961 tatccgttac tagtgatttc acaaatgttg ccaatcatgg tgttagggcc attacttacc
     7021 ctatggtttg gtttcggtat gcttccaaaa gtgatccttg ttgtcttgat aagctatttt
     7081 ccgatcgtag tttctttttc ggatacattg aggaaagtat caaaagagca aatcatcttt
     7141 ttgaaaacga tgggggcaga tacagggaag atttatcgaa tatataaaat accagcgggt
     7201 ttatccggct tcttttccgg tttaaaagtg gctgctactt attgtgtcag cggagcagtc
     7261 gttggagaat ggctaagcgc tcaagcaggc ttagggtatt acatgatacg cgtaaaaaac
     7321 agctaccaga tcgataaagt ttttgctgcg atcatttgtg tcattctatt gagtttattg
     7381 ctgaatggcg cttgttcatt attgaaaaaa gggtattatc gtatgcttta tattttttaa
     7441 gagaagggga tcaaatgaaa aaattatggc ttttattacc gctactgttg cttttatccg
     7501 cttgtggcac tgcaaaagaa acaagtagca aacaagaaat aaaggatttg aaaaaagtga
     7561 cacttgtttt ggattatgta ccgaatacta atcatactgg catctaccta gcaaaagaaa
     7621 aaggctatta taaagaggct ggtttaaatg tccaaatcat cgaaccagga gataattcga
     7681 caagtattgg tctagtaggt gcggacaaag cacaatttgg tgtaagttat caagaagatg
     7741 tcacttatgc tcacgcagat ggacagaata taccagtcaa agcaattgct acagtgatca
     7801 aacataatac ttctggattc gccaccctat cagacagtaa catccattca ccaaaagatt
     7861 ttgaaggaaa aacatatgca ggttggcaaa gtccaagtga agaagctgtc ttaaaagctg
     7921 tgatggaaaa agacggcggg gacttttcaa aactgacaat ggtaggaagc aatggagaag
     7981 ggccagaaag cttaggaaaa tcctcagata tccaatggta ctttgaaggt tgggatatga
     8041 tcaaagcaaa agaagcgggt attgaactaa actatatccc attgaaagaa ttagatgaac
     8101 ggttagatta ctatactccg gtaattatta cgaatgatca actgatcaaa agtgatccag
     8161 aattggtaca gtcttttatg gatgccacga aaaaaggcta tcaggaagct attaaggatc
     8221 caaatgacag tgctaaactg ttgcaaaaat atgcaaaaga aaatgatcgg acgtttttag
     8281 aagaatcaca agcattcttg tcaaaaaatt atacggatga tcctgagaat tggggattga
     8341 tggaagaaaa agtttggcgc aattatacag cttttatgca ggaaaacgga ttgatcaagc
     8401 aagatgtacc ttcgcaagaa ctattcacta atcagtttat aaaataggag tgatagatat
     8461 gtcaatccaa atcagccact tgaatgtagt cattgaaaac ctgaaaatat tagaagacat
     8521 ccatttcact atccctaaag atacctttac atcgattgtc gcgccaagcg gagccggtaa
     8581 gtcaacattg ctgaaaactt taacaggagt ccagcctatg acttctggaa cgatcaaagt
     8641 agacgatcaa aaagtaactg gtctccatac agcatttagt tatatgccgc aagaagatat
     8701 gttacttcct tggctgagcg tctatcaaaa cgttacgtta tatcaaaaaa tcaatcatct
     8761 aacaatcaat gaagaacaag taagggatta tctggatatc tttgggttaa acggctatga
     8821 acattttttg ccggaacaat tatctggcgg gatgaagcag cgaacggcct tgttacgaac
     8881 aatcatgaat ccttcttctt acttgcttct tgatgaacca tttggagcgt tagatgctat
     8941 gaccagaggg cagatgcaag actggatgtt gaagctgcct aaaaaagcaa agcggactac
     9001 tttacttgtg acgcatgata tcgaagaagc aatttattta tcagacagga ttcttgtgct
     9061 ttccgcccgt ccggcacatg tgattgctga gattcaagtg cctgaaaaac gacggtcacg
     9121 tgaatggcta ctgcagcaat cagagttgaa gcaaacgatt tatcagttat tggcaggtga
     9181 attagatgtt aagtgatgct cattgtcatt tagatgggag tcagagttta gcactccttc
     9241 agcaggaaca cacgattttg acgatcatca attgtgattc gccagaagaa tggaaggaaa
     9301 acagacagct tgcagcaagt aagactcaag ccttaagtta cggtatccat ccttggaaag
     9361 cagatagcta tacgtttgaa caagttgagc cttttttgaa aaaagcaagg atcatcggtg
     9421 aaatagggtt agataatatt tggacgaatg taccgatgac tacacaaaaa aaagtatttg
     9481 agcgacagct tgcttttgca gccataaatg agaaacctgt tgtactgcac acaaaaggat
     9541 gcgaaaaaga gatcttaaat tatatccaag cttaccctaa tcactatttg atccattggt
     9601 attcttcttt ggactatcaa caagactata ttgatttagg ctgttatttc agcattggtc
     9661 ttgatttgaa aaagaatcca gcagtctggc aactagctaa agcagtccca atcgaccact
     9721 tacttattga aacggatgga ctacatgcag cagagtgggc tttagggggg attttttcag
     9781 aaaaagatta tgtctcactg cttgaagaac agttaaagac aatcgctgag atcaaagagc
     9841 tatcagttga agaagttaaa aaaaatactt atcaaaactt ggaacgtttc attagattag
     9901 gtaaataaaa aagagatgtg acacaactag tgtcgcacct ctttttggct ttctttgtat
     9961 aaaagtaaac tgtttgatta ataaatgaat cttagtacca accgtttgct tcccagaatg
    10021 ctttcgccgc ttcccaagaa ccataacgtg aagtaacata ttgttctgct actctttctt
    10081 ggtttgcagc agagtagtca ccatttaagt aagaagaatc taattggtaa cgtccgatgt
    10141 aacgtccgtt cgttgctgtg taagaaccgc ttgattcttt ttgtgcgatc cattcttttg
    10201 cagagcttgt gcttgctgga gtagcagcag tttctgttac agcaggagca ggttgtgttt
    10261 cagcaacagg cgctgcttgt ttttccgttt gaaccgtttc ttgtacaaca ggttgttcag
    10321 cttggactgg tgcttcttga acggctgctt gaggttcagc agctgcttgt gcagagcctt
    10381 ctgttgggat tgttaattgt tgtcctgagt a
//
LOCUS       DACECY010000081        10379 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.086, whole genome shotgun sequence.
ACCESSION   DACECY010000081 DACECY010000000
VERSION     DACECY010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.086"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IWO38_002398"
     CDS             6..305
                     /locus_tag="IWO38_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0314450.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IWO38_002399"
     CDS             378..1739
                     /locus_tag="IWO38_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ0314451.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IWO38_002400"
     CDS             1761..2021
                     /locus_tag="IWO38_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314452.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IWO38_002401"
     CDS             2035..2787
                     /locus_tag="IWO38_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ0314453.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IWO38_002402"
     CDS             complement(2881..3486)
                     /locus_tag="IWO38_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0314454.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IWO38_002403"
     CDS             complement(3539..4513)
                     /locus_tag="IWO38_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ0314455.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IWO38_002404"
     CDS             complement(4891..5670)
                     /locus_tag="IWO38_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0314456.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWO38_002405"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWO38_002405"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0314457.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IWO38_002406"
     CDS             7161..8558
                     /locus_tag="IWO38_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0314458.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IWO38_002407"
     CDS             8639..9079
                     /locus_tag="IWO38_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0314459.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IWO38_002408"
     CDS             9227..9553
                     /locus_tag="IWO38_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0314460.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IWO38_002409"
     CDS             9554..10303
                     /locus_tag="IWO38_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314461.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELIGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         3292 a         1832 c         2150 g         3105 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaattta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg ataggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtct
//
LOCUS       DACECY010000082        10131 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.087, whole genome shotgun sequence.
ACCESSION   DACECY010000082 DACECY010000000
VERSION     DACECY010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.087"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            120..626
                     /locus_tag="IWO38_002410"
     CDS             120..626
                     /locus_tag="IWO38_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ0314462.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            643..1542
                     /locus_tag="IWO38_002411"
     CDS             643..1542
                     /locus_tag="IWO38_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ0314463.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1629..2513
                     /locus_tag="IWO38_002412"
     CDS             1629..2513
                     /locus_tag="IWO38_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ0314464.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2705..2776)
                     /locus_tag="IWO38_002413"
     tRNA            complement(2705..2776)
                     /locus_tag="IWO38_002413"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2741..2743),aa:Arg,seq:ccg)
     gene            2836..4170
                     /gene="rpoN"
                     /locus_tag="IWO38_002414"
     CDS             2836..4170
                     /gene="rpoN"
                     /locus_tag="IWO38_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ0314465.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4373..5410
                     /locus_tag="IWO38_002415"
     CDS             4373..5410
                     /locus_tag="IWO38_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ0314466.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            5451..6452
                     /gene="gap"
                     /locus_tag="IWO38_002416"
     CDS             5451..6452
                     /gene="gap"
                     /locus_tag="IWO38_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ0314467.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6562..7752
                     /locus_tag="IWO38_002417"
     CDS             6562..7752
                     /locus_tag="IWO38_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ0314468.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7856..8611
                     /locus_tag="IWO38_002418"
     CDS             7856..8611
                     /locus_tag="IWO38_002418"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ0314469.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8727..10025
                     /gene="eno"
                     /locus_tag="IWO38_002419"
     CDS             8727..10025
                     /gene="eno"
                     /locus_tag="IWO38_002419"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ0314470.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3548 a         1869 c         2099 g         2615 t
ORIGIN      
        1 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
       61 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
      121 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
      181 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
      241 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
      301 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
      361 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
      421 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
      481 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
      541 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
      601 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
      661 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
      721 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
      781 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
      841 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
      901 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
      961 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
     1021 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
     1081 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
     1141 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
     1201 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
     1261 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
     1321 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
     1381 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
     1441 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
     1501 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
     1561 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
     1621 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
     1681 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
     1741 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
     1801 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
     1861 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
     1921 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
     1981 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
     2041 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
     2101 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
     2161 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
     2221 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
     2281 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
     2341 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
     2401 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
     2461 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
     2521 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
     2581 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat tttctttggc
     2641 ggacagtttt cacttaaaaa taaagaagca actattcgta aattatgcta aaatagcagt
     2701 agaacgcgtc ctgagggaat cgaacccccg tctcaagaac cggaatctta cgtgatatcc
     2761 actacactaa ggacgcaagc actgggataa ttgtaactaa acagataaaa aattacaagg
     2821 ggattggaca aaaaaatgaa atttgagcaa aatttttcac aaaaacagca gcagacacag
     2881 aaactagcca tgacgcaaca gttgcaacag tccatccaag ttttacaata taacagtgag
     2941 gagctgttgg cattcgttga gaatcaagca atggaaaatc cattggtgga agttgttgag
     3001 ccggaatggc agccagatta cataaaagct tcttcatctt cttatgaagg agaagagaca
     3061 aattacctaa atcagattcc tgatacaaaa gggtctttat ttgattcatt gatcgaacaa
     3121 gtccatttga attaccgaga tacttttttg aggaaaatcg ttctttatct tgtagaatac
     3181 atcgatttga acggattttt gacgattagt ttagaagaag ccggaaaaga gattggagct
     3241 acgcctatcc aaatgctcga tgcgttgact ctgattcagc aattagaacc agctggagta
     3301 ggtgcacgta gtttgcaaga atgtctcatg cttcaaacag aacgagatga ttatgcgccg
     3361 gaattagctt atatcgtgtt ggaagaatgt tttgaggaac tggtggagcg aaaatggaaa
     3421 gaaatcgcac aacgtttttc tgttgatcta catgctgttc agcagatatt cgactattta
     3481 caaacgttga gtccaagccc tggccggatt ttcgaccgta gcagcgaatt gtacattatt
     3541 cctgatgtcc gggtattagt ggacgacgat aaaaatgtac aagttatctc aaaccgaaaa
     3601 aatcagccaa atatacgctt tcaagagagt tacttcaaac aaatgtcaca acaagcagat
     3661 aaagaaactg aaaattattt aaaagaacgg aaacaggaat tcgaatggtt gaagaaaacg
     3721 atcctacaac gtggggatac aattttgcgg gttgctcaag tcattgtttc ccggcaaaaa
     3781 gctttcttta tcgacaagga acgtccaatc aagcctctta cgttaaagga agttgcaact
     3841 gagatagatg tgcatgaatc cacggtcagt cgtgcagtca acgggaaata tttggaaaca
     3901 gacttcggtg tatttgaatt aaaaaaattt ttcactacac gaattcctac gaatagtaca
     3961 gaacagacag aagacctttc agcagataca gcgaagaaaa agctgcagga attagtcgat
     4021 caagaagaca aaaacaagcc gttgtctgac cagaaattgg ttgaattgct gaaaaaagat
     4081 gagatagcga tctcaagacg aacagtagca aaatatcgtg atttattagg aatcccgagt
     4141 tcatcaaaaa gaaaacgtta tgataactaa tcagtcaaaa gactagaaaa agtatttcac
     4201 tttttctagt ctttttagtt gaaaaacgag agcaaaactg ttagacttac agtcgtgagg
     4261 ttgagatttc cctttattga tgtgaaattc gaatagttaa tttctttttt tatcgtatat
     4321 gggtcgtttt aagtctacat tggacgtaaa gtgtccaact aaggagatcg ctatgctgga
     4381 tgaaataaaa atgattgaat ctgttgcacc agatattatc gaagtgttgc aagaacgcta
     4441 taaaatttta cgaaatatct attggatgca gccgattgga cgtcgaagtt tatcggaaag
     4501 tatggggatc acagaacgag tattgcgaac agaaacagat cttttgaaaa atctgaattt
     4561 gatcgacacc tcaaaaagcg gtatgacgct gacaactaag ggcgaagaag tttatcaaag
     4621 ccttgaaaac ttcatggatc agcttttagg tacgcatcaa accgaacaac agttggcaga
     4681 atattttggt aaccaacgct gtatcgttgt ttcaggaaac agcgaagagc agaccaaagt
     4741 ggctgatgca ttcggcgaag cgctgagcga agccctagac cgactacttc cagaaggcga
     4801 gaatatcatc gctgtgatgg gaggaactac tatggctgtg gtagccgaac aattgtctaa
     4861 tctggaaaat aagaaacggc ataacctgtt tgtccctgca agaggcggga ttggtgaagc
     4921 gataactgta caggcaaatt ctgtcagtgc aagaatggct gctaaagcaa acggtaacca
     4981 ccgtgcactt tatgttccgg aacaattaag tctggcaact tacaattctt tactcaatga
     5041 gccatctatc caagaagtct taaacttgat tagtgaagcg aattgtgtta ttcatagtat
     5101 tgggcgtgca ttacacatgg cagcacgtcg taagatgact gaaaaagaat tagtcatgct
     5161 gaaacaagca aatgctgttg cggaatcttt tggctacttt ttcaatgaaa aaggtgaagt
     5221 cgtttataag gtccctagaa tcggcataga attgcaagat ttggaaaaag tcccgatcat
     5281 catggcaatc gcgggcggaa aatcaaaagc taaagcaatt cgagcttata tgaaaaatgc
     5341 accaaaacaa acgtggctca tcactgatga agctgctgca aatgagattt taaaaggggt
     5401 aaccctttaa aataaatatt tttttgattt tcataaggag gaaatcttaa atgacagtta
     5461 aagtaggtat taatggtttt ggacgtattg gacgtctagc attccgtcgt atccaagatg
     5521 tagatggaat cgaagtagta gcaatcaacg acttgacaga tgctaaaatg ttggcacact
     5581 tgttaaaata cgacacaact caaggacgtt tcaacggaac agttgaagtt catgaaggtt
     5641 cattcaacgt aaacggaaaa gaagttaaag tattagctaa ccgcaaccca gaagaattac
     5701 catggggcga actaggcgta gatatcgttc tagaatgtac tggtttcttc acttctaaat
     5761 cagcagctga aaaacattta acagctggtg ctaaacgcgt tgttatctct gctcctggcg
     5821 gaaacgatgt tccaacaatc gtttataaca caaaccacga aacattaact ggtaaagaaa
     5881 cagttatctc aggtgcttca tgtactacta actgtttagc tcctatggct aaaacattga
     5941 acgacaaatt tggtgttgtt gaaggattaa tgacaactat ccacgcttac acaggtgacc
     6001 aaatgactct agacggacct catcctaaag gtgacttccg ccgtgcacgc gctgctgctg
     6061 caaacatcgt tcctaactca acaggtgctg ctaaagctat cggtttagta attcctgaat
     6121 tgaacggtaa attagacgga gctgctcaac gtgttcctgt accaacaggt tcattaacag
     6181 aattagtaac agttcttgaa aaagaagtaa ctgttgacga aatcaatgca gcaatgaaag
     6241 aagcttcaaa cgaatcttac ggatacaaca cagacgaaat cgtttcttca gatatcgttg
     6301 gtatgactta cggttcatta tttgatgcta cacaaactaa agtaatgaca gttggcgaca
     6361 aacaattagt taaaactgtt gcttggtacg acaacgaaat gtcatacact gcacaattag
     6421 tacgtacttt agaatacttc gctaacttat aagatagaca aacgtacaag tgtaaattat
     6481 agacatgtac atttaaaagc ggggaagcaa tcgcgcttct tccgcttttt ttgataataa
     6541 gtttattcag gaggaaataa aatggctaag aaaacagtga aagatatcga tttaaaagac
     6601 aaaaaagttc ttgtccgtgt tgacttcaac gttcctttga aagacggcgt gatcactgac
     6661 gacactcgta tcaaagcagc attgccaaca atcaactacg ttctagaaca aggcggaaaa
     6721 gcaatccttt tctctcacct tggacgtgta aaaacagaag aagataaaga aggaaaatct
     6781 ttagctccag ttgcaaaacg tctaggcgaa ttattaggta aagaagtaac attcgtacct
     6841 gaaactcgcg gagaacaatt agaagaagca atccgcaaca tgaatgacgg cgacgttgtc
     6901 gtattcgaaa atacacgatt tgaagatatt gacggtaaaa aagaaagcaa aaatgataca
     6961 gaactaggta aatactgggc ttcattaggc gatgtatttg ttaacgatgc tttcggtaca
     7021 gctcaccgtg cacatgcttc taacgtaggt atcgcttcaa ctggtatccc aacagttgct
     7081 ggattcttga tggaaaaaga aatcaagttc atcggtgaag cagtagaaga accaaaacga
     7141 ccaatgattg caatccttgg tggcgcaaaa gtttctgata aaatcggcgt aatcgaaaac
     7201 ttgattccta aagcagataa aatcttgatc ggtggcggaa tgacttacac attctacgaa
     7261 gcaaaaggaa tcaagatcgg taactctcta gtagaagctg ataaagtgga actagcaaaa
     7321 gaattaatcg aaaaagcagg cgacaaactt gtcttgccaa tcgacaatgt ctgtgcacca
     7381 gaattttcaa atgatgttga aacacaagta atcgaaggag acattcctga tggtttgatg
     7441 gctttagaca tcggcccagc atcagtaaaa ttatttgctg atacattaca aggtgcaaaa
     7501 acagttgttt ggaacggacc tatgggtgta tttgaaatga gtaactttgc taaaggtaca
     7561 atcggtgttt gtgaagcaat cgctaactta gaaggcgcaa caacaatcat cggtggcgga
     7621 gactcagctg cagcagcaga acaattagga tttgctgaca aattcacaca catctcaaca
     7681 ggtggtggcg caagcttgga attgcttgaa ggtaaagaat taccaggact agcagcaatc
     7741 aacgataaat aaacgaccaa gtttgctcaa ttctgacgag taatcgatca aaaggtgaag
     7801 ggagttttca tgactcccta gccatttttc aataaattaa ggagtgtgtt cctcaatgcg
     7861 taaacctatt atcgcaggta actggaaaat gaacaaaact gcatcagaag caaaagaatt
     7921 tgctgaagca gtaaaaaaca aaatcccttc aaatgacaaa gttgattcag tcatcggatc
     7981 tcctgcatta ttcttacaag aattaatgac atcagctgaa ggtacagaac taaaaatctc
     8041 agcacaaaac tgctactggg aaaactcagg tgcgttcact ggtgaaactt caccagcagc
     8101 tcttgctgat ctaggtgttc attatgtgat catcggacat tcagaacgtc gtgaatattt
     8161 ccatgaaaca gacgaagaca tcaataaaaa agcaaaagct atctttgcaa atggtatgac
     8221 accaatccta tgctgcggcg aatctttaga aacctatgaa gcaggcaaaa ctgctgaatg
     8281 gatcgaagga caaattacag ctggtttagc tgatctttca gctgaacaag tgtcaaacat
     8341 ggttattgct tatgaaccaa tctgggcaat cggtactggt aaatcagctg acgcacaaat
     8401 cgctgacgaa atttgtggag ttgtacgtca aacagttgaa aaactttatg gaaaagaagt
     8461 atcagaagca gttcgtatcc aatacggcgg ttctgtaaaa ccagaaaata ttgctgaata
     8521 catggcaaaa gaaaatgttg acggtgcttt agtcggtggc gcaagcttgc aagcagattc
     8581 atttttgtca ttgttagacg ctgtaaaata attgttgaaa atttgcgaaa aatgcaagaa
     8641 atgtttgcac taatgagcaa aattaactaa aatcgtatat gagggaaatc ccttaggaaa
     8701 aaacaaactc aaaggagaga caaaacatgt caattattac tgatgtttac gctcgcgaga
     8761 tcttagactc acgtggtaac ccaacaatcg aagtagaagt atacacagaa agcggagctt
     8821 ttggccgcgg aatggttcct tcaggtgctt caactggtga atacgaagca gttgaattac
     8881 gtgacggaga caaagctcgt tatggcggta aaggtgtaac taaagctgtc gacaacgtaa
     8941 acaacatcat tgctgaagca atcattggct acgatgttcg tgaccaaatg gctatcgaca
     9001 aagctatgat cgctttagac ggaactccta acaaaggtaa attaggtgcg aacgctattt
     9061 taggtgtttc tatcgctgtt gcacgtgcag ctgctgacta cctagaagta cctttatacc
     9121 actacctagg cggattcaac acaaaagtat tgccaacacc aatgatgaac atcatcaacg
     9181 gcggatcaca tgctgataac tcaatcgact tccaagaatt catgatcatg cctgttggcg
     9241 ctccaacatt caaagaagct ctacgtatgg gtgctgaagt attccacgca ttagcttcaa
     9301 tcttgaaagc tcgcggatta gcaacttctg ttggtgacga aggtggattt gctccaaacc
     9361 ttggttcaaa cgaagaaggt tttgaagtaa tcatcgaagc aatcgaaaaa gctggctatg
     9421 tacctggtaa agacgttgtt cttgctatgg atgctgcttc ttcagaattc tacgacaaag
     9481 aaaaaggtgt ttacgtacta gctgactcag gcgaaggcga aaaaacaact gacgaaatga
     9541 tcaaattcta cgaagaatta gtttctaaat atccaatcat ctcaatcgaa gatggattag
     9601 acgaaaacga ctgggacgga ttcaagaaat taactgacgt attaggcgac aaagttcaat
     9661 tagttggtga cgacttgttc gtaacaaaca ctcaaaaatt atctgaaggt atcgaaaaag
     9721 gaattgctaa ctcaatcctt atcaaagtga accaaatcgg tacattaaca gaaactttcg
     9781 aagctatcga aatggctaaa gaagctggct acacagcagt tgtatctcac cgttctggtg
     9841 aaacagaaga ttcaacaatc tctgatatcg ctgttgcaac aaatgctggc caaatcaaaa
     9901 ctggttctct atcacgtact gaccgtattg ctaaatacaa ccaattacta cgtatcgaag
     9961 accaacttgg tgaagttgca gaatacaaag gtttgaaatc tttctacaac ttaaaaaaca
    10021 aataattctt ttctaatgaa gatacatgat cgactattga aagatagtcg agtatgatca
    10081 cttagttaag taatcgaact ctctacttgc tgaaatatca gcaggtagag a
//
LOCUS       DACECY010000083        10084 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.088, whole genome shotgun sequence.
ACCESSION   DACECY010000083 DACECY010000000
VERSION     DACECY010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.088"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(245..757)
                     /locus_tag="IWO38_002420"
     CDS             complement(245..757)
                     /locus_tag="IWO38_002420"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ0314471.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(771..3068)
                     /gene="recJ"
                     /locus_tag="IWO38_002421"
     CDS             complement(771..3068)
                     /gene="recJ"
                     /locus_tag="IWO38_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ0314472.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3210..3644)
                     /locus_tag="IWO38_002422"
     CDS             complement(3210..3644)
                     /locus_tag="IWO38_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ0314473.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3654..4442)
                     /locus_tag="IWO38_002423"
     CDS             complement(3654..4442)
                     /locus_tag="IWO38_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0314474.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4439..5380)
                     /locus_tag="IWO38_002424"
     CDS             complement(4439..5380)
                     /locus_tag="IWO38_002424"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ0314475.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5557..6144)
                     /locus_tag="IWO38_002425"
     CDS             complement(5557..6144)
                     /locus_tag="IWO38_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ0314476.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6365..7678)
                     /gene="obgE"
                     /locus_tag="IWO38_002426"
     CDS             complement(6365..7678)
                     /gene="obgE"
                     /locus_tag="IWO38_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ0314477.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7966..8976
                     /gene="gap"
                     /locus_tag="IWO38_002427"
     CDS             7966..8976
                     /gene="gap"
                     /locus_tag="IWO38_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ0314478.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9061..9996)
                     /locus_tag="IWO38_002428"
     CDS             complement(9061..9996)
                     /locus_tag="IWO38_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0314479.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2777 a         2298 c         1746 g         3263 t
ORIGIN      
        1 ttacgggggt gtgacaaaaa tcggtcacat cctttttttc gaataaacgg tgttctaaag
       61 ccagtttttc ggtattaagt caaattgcgg aaaaagtgat tttttaaact tctactggtc
      121 tttgcttgtt tccatttata aaaaaatagc gaccctcaaa agtcagatag agaatctgac
      181 ttttgagggt cgctttaaaa tcgcttcttt aagaataaca tcaagaatag cttctttttt
      241 ctgcttaata ttccattaat gtaatgatgt catatccttt gattttgtcg cgtccatgga
      301 gatccatcaa ttcgatcaaa aatgcgcagc ctacgactac accgcctaat tgttcgatca
      361 gttcgatcgt cgctttgatt gttcctccag tagctagcaa atcgtcacaa atcagcacac
      421 gttgacctgg tttaatggca tccttatgca atgtcaatgt atctgttcca tattctaatc
      481 cataagtcac ttcgatggtt tctcgaggta acttcccttt tttgcggact ggggcaaaac
      541 cgactcctaa ctcataagca acaggacatc ctacgatgaa tccacgggct tctggcccaa
      601 ctaccatatc aattcgtttt tcttttgcat aatttacgat ttgtttagta gcttcccgat
      661 aagcgtcacc atctgccatc agtggggaaa tatctcgaaa cacaattcct tttgacggat
      721 agtcgggaat actggcaata taatctttta aatccactct ttgtttcctc ctaggttgtt
      781 aaccatttct ttatggtagg aatatcgctc aaaagcaaaa attcctcaac tttgatttta
      841 ttgagccgtc gctggtataa tctgctctca gtcaaaggat gactttctgg gctagcgact
      901 ttttgtaaaa caccgttctc tattgtaaca aatttcaatt caaaaaacac ctgtatcata
      961 aaaattaata atttttcagg gatacgcaaa tattgagcta ttgcttttat tttatgacgt
     1021 atatcaattc gttcttgaga atggatcaat ttaaacaatt ttgcgtactg ttctcttgtg
     1081 cctaccccat tcaaataggc ctcatcaggt gaaatcccca tcagataaac acgattgaat
     1141 gaataagacc gaaaaacttc tttcagatcc gctaaatcat ctggacaatc cataacaact
     1201 agagagtttg gttttctttt ttcaattatt gcctttacag aatctagtcc ttcgaaaaca
     1261 atcgcagctt cctggataat ctcaggcacg aatttcaacg tcgaagaatc aaatgctaaa
     1321 taaaggctac cttccgacgt tcggctctgt tcacggaaac gttttgcccg ccaatcaaat
     1381 acttgcagac cacccacaga aaaatcagat accatcagct gcggtttctt acgcccgttc
     1441 cattcattga tcgaaagctt tcctaccaca tctacctgat tattcagcag ttcttcttct
     1501 tgagtaccaa aaccaaaagc gaccgcatca agctgactcg atgcatcgga aaggacaaat
     1561 ttcagatgct gttggtttgc tccgattctt ttgatatttt ctgcttggac ttgtcgaaat
     1621 aaaaactgag gcaaaggatt atccgtacca aatggcgcca atagtttcag ctcatcaatt
     1681 cgctctactg ttacttcatt tggcagcaat acttcatcaa ttctcaactc aggccctctc
     1741 atcaagtcaa tttgatgatc gactatatat tggttcatct tctcctgtag tatagtgaca
     1801 ttttcacttg gcatcgttaa accgactgct gcatgatgtc ctccgaagta agtaaataaa
     1861 tctcgcatct ggtcaagcat ttcaaaaaga ttcaaggctt caatactacg tccagatcct
     1921 ttagctgatc catcttcttt taaagtaagg acaagcgttg gtttacccgt ttcattcatt
     1981 attttcccag caacaatgcc caaaacgcct tcatgccatc ctcggtttac caatagatgg
     2041 atatgattct ccttattgac catcgctaat gcttcttgtg ttatctgctc tacgattgct
     2101 tttctttctt cattgatctc attcaatttt tttgcttgaa cggatgcttc ctcttcatca
     2161 aacgtcgcaa gtaacgagac ggcaggacgt ggatcgccca tccgaccgat tgcatttagt
     2221 ctgggagcaa tcgaaaaacc gatcgttgtt tcgtctgcgt ccctcatttt tacgccgctt
     2281 tcttcaaata gcgcctgaag tcctatccgt tctgaatgat gaatggcatc gattcctaat
     2341 gaaacaagga ttcgattctc atcagtcaat gaaactaaat cggcgatcgt tcctatcgct
     2401 actaaatcca aaaattctgc aggcggttct tccaataatg cagaagctac tttgaatgct
     2461 actccgactc ctgccaaatc tggaaatgga tagtgccccg ctggatgacg agggtgaaca
     2521 atcgcataag cgtctggtaa attctcaggt agttcgtgat gatctgtaat gatcacgtct
     2581 acaccagctt gctgagcgta tgctaccgca tcatttcctg ctaccccgtt atcaacagtg
     2641 atgatcaact ggattccttc ttcgatcttt tcttggtata ccgattggtt tggtccatac
     2701 ccatgctcaa atcgatttgg caggaatact tctacttctg caccaagtaa ttccagtgtt
     2761 tctttcatta ccgttgcact ggtgatccca tctgcgtcat aatcaccgta aacgaggatt
     2821 ttttctccac tgattacagc ttcttggatt cggctgattg ctttatccat atcgtgtatc
     2881 agatacggat catgtaattc ttgttccttt ggatgtaaaa atcggttgat atccttttcc
     2941 tcatggtaac ctctttgcca cagtaactta ccgacaaatg gagataattt cagttcacgg
     3001 atcatttgga gaaaggattc agaaggctct gtctcgctta acttccaatc aaaatttgct
     3061 tgtctcactt tctcactcct aacttagtta ttatagcaaa ggaaatcagc aaaaaaaagc
     3121 agaaagaaag aataaaaaca gatcagatcc gctagacaga tgttcagcag gacctgatct
     3181 tttgtacgac catttatgaa atatccctat tagtcaaaat aattgaagct ttgtttgggc
     3241 gattgagttt tttgttcttc cggcgctttc aattgactga tttgtgcggt tttcgcttgt
     3301 aactccgcct gataagcagc ttccaactcc gcacgttcgt ttctaaactc gcgttgctgt
     3361 tcttcttttg cttcttcaat tttcttttga agttctgttt gatattctga catttcctga
     3421 gtcagctgtt tgatttgctt tttttgctgc catagtgttg ccgaagcagt caaaaataca
     3481 atcagtgcac caatcagcgc agaaccgatg atcactaaga tcaatggcgc agaaaaggaa
     3541 gtgaagccaa aacttacagg aacattttta ttgttcaata cagcaaaaag aacaataatc
     3601 aaaactaaaa caaatccaag aatcacattg ctttgctttt tcaattcctt cacctacttt
     3661 ttgttaaaaa ttccgctggc taaataatca ccaatcgttg gaaacagcgt ataaaatttt
     3721 gatgcaactt ctaaaataaa cggttgattg atttcccgtt ttggatgtct catattgcgg
     3781 acgatcattt ttgccaactt ggcaggttct aaaacgattt gtccgacttt ttccaaatag
     3841 cttccgcttg gatcagcctt atcgaagaaa tttgtctcga ttggacctgg attcactgtc
     3901 gtaacagcta cgccaagtgg tttcaattct aatcgcaaag cgttggagaa gccaaggact
     3961 gcaaatttcg tggcagaata aaccgttgat tttgcagttg ccatttttcc agccatagag
     4021 gcgatattaa tgatatgacc ttgtcttctt tctgccatat cgatcgcaaa tttttgtgtc
     4081 aaaaccatca ttcctaagat gttgacttca aacatatcgt atgcttttcc aaggtcgaat
     4141 gtaaggaagt cttcgaaaat cccaaaaccc gcattgttta ccagaatatc gacgcgcccg
     4201 acttcttcca tcaccttttc atataattcg tccacgcttt caggattgct gacgtctacc
     4261 ttgaaagcat aagcctcttt gccgctcaag cgtctgcact cgtctctgac accttctaca
     4321 aatgccattc ttcgtgcgca acttataacg attgctcctt gctttgctgc ttcataacag
     4381 atttgtgcgc ccagaccgcc tgaactacct gttactaaaa caactttatc ttctaaattc
     4441 atgcctcttc ctcctcgcga aatggaatct caatgctatc aaaatctttc ataatcgtcg
     4501 tgttttcaaa cacttcttgt gcttcttttt caagctctaa agctgctttt cctaaatatc
     4561 tcgcactgat atgagtaagt agcagccgtt ttgcctgcgc ttcttttgca acttcagcag
     4621 cttgatgagt cgtggaatgg aaatagttgt gcgccattct tgcttcatct ttattaaatg
     4681 tgctttcatg taccagaata tcgctatttt ccgcaagcac aacggaatta tgtgtttttc
     4741 tcgtatcgcc taaaatagtc acgattcgtc cttttttatc tggacctacg aaatcttttc
     4801 cattgatcgt ccgcccatct tctagttgga tcgtttcacc ttgcttcagt ttgccgtaca
     4861 gtggtccagc tggaatacct aattctttta gacgatccac ctgcaattct cctttatgat
     4921 catgttcaac cacacgataa ccaaaactgt cgatcccatg atttagtttt tttgtataaa
     4981 cagaaaattg ctgatcagta aaaatcgggg ctgtttcatt tagctcaatg aatttcagcg
     5041 gataacttaa acgcgtttgc gaaataccta aagagacttt tatgtattcc tctatccctt
     5101 ttggcccgta aatctctaat ggcgtatccc caccttgaaa tgaccggctg ctgatgagcc
     5161 cgggaagtcc aaaaatatga tcgccgtgta aatgtgtaat gaaaatttta ccgatttttc
     5221 tcggacgaat agtcgttcgc aaaatctgca tttgcgtgcc ttcgccacag tcgaacaacc
     5281 aaacttcatt gcgctcgtct aaaagtttca atgctatact tgtcacattt ctatgttttg
     5341 caggaacacc tgcacctgtt cctaaaaatt gtaattccat aatctacctg actttcttta
     5401 agttcctctt taattttagg gtaaaaagaa gggataatca accgtaagtc gagaatgggt
     5461 tcaaattaaa aataacgttc taaagcgata gcgttttctt tgaaaattac tataaaaaca
     5521 gcagcgaacc cgtttgagat cctctgctgt tttttattaa tttgaagatc cagaactttc
     5581 attgtcgttg gaagtttgcg gatgtttgtc cttccaagtc ttttgtgcag cttctttcgc
     5641 atgattttca gcttctttcg catcatgcac atctttttcg acatcttcat aatccaagcg
     5701 agtaatttct ccgcctaatt ccatcattac caattgattc agtgggcgat tgtcctcttc
     5761 atcagcaatc aagatcagtc ctgtagctcc ttcaccgatt ttatttccta caaagtcgaa
     5821 cacggtctgg gcatctttta tttctttggc atcttgtgtc gcaccgatca ttcctccggc
     5881 aaaccatcct aataaaatcc ctagcggacc acctaataca ccaaccaaca ttccgattaa
     5941 tccacccgta gaagatttgt tattaccggt aaagtcgata aaatcttcta ttttgaattg
     6001 atgtgtccca ttaggatcat ggctgacaac tgccatctgt tctcctttaa tcgaacgaac
     6061 agcatgtaac tttttgattt ctgaaaatgc ctgatatgcc tggctttcta cttgaaaatt
     6121 taaaatgatt actcgtttgc tcattaaaaa cgcccctttc ataccttctt gttatttatt
     6181 ttacctttaa ttgtaaaata agaaaagaaa agcactcttt ttttccaata aaatagggct
     6241 gtgacaaaag tcgtatcaca gctccttttt ccgaataaac ggcgttcttg aagctgatca
     6301 cttctgtcac aacctccctt aaatctgaat ctatattttt tggtatttgt ttttatttgc
     6361 ttgcctattc gacaaattca aattcaaact caccgatacg tacaaggtcg ccatctttcg
     6421 caccgcgtgc ccgaagagct tcatccacac ccataccacg taattgacga gcaaaacgca
     6481 tcactgtttc atcatgatcg aaattcgtca tttggaataa tttttcgatc ttctcaccag
     6541 ataagatcca cgttgcatct gaatcacgat cgatttggaa ctcaggacct tctggattga
     6601 atccatagtg tacagtttct tcttccagtt cttcttcgta caatgggaat tctggcgtta
     6661 cttctaacaa atctgctgta gcattcaata acgcgtccaa tccttgtcga gtcactccag
     6721 aaattggaaa tactgggatg tcatcagcaa attcatcttc tttttccttg ttcagctgtt
     6781 ctttgaattt cactagattt tccggtgcat ccggcatatc cattttgttt gccacgatga
     6841 tttgcggacg ctctagcaag cgtaagttat aagtagataa ttctttgttg atggcaagat
     6901 agtcttcata aggatctcgt ccttccatcc cactcatatc gatcacatgt aaaattactc
     6961 gtgttctttc gatatgacga aggaattgtg tacccagccc aacaccttgc gaagctcctt
     7021 caatcaagcc tggcaagtct gctgcggcaa aacttctgcc atcacttgta gtaaccattc
     7081 ctagattagg tacgagcgtc gtgaaatgat aagcgccgat ttttggacga gcagatgaaa
     7141 taacggatag taaagtcgat ttcccgacag atggaaaacc tacaagtcca acgtctgcca
     7201 atactttcaa ttctagttcg atttttcgct cttgtcctgg ttcaccgttt tcagcaattt
     7261 ccggtgcagg attccgagga gaggcaaaac ggatattccc tcgtccaccg cgtccacctt
     7321 ttgccacaac tagtgtttga ccattttcaa ttaaatctcc taaaagagca ccagtctccg
     7381 catctcggac agtggttcct tgtggtactt taacataggt atcttctgaa ccacgaccgt
     7441 gcatcccttt actcatccca ttttcacctg gctgcgcttt gaaatggcga ttgaaccgaa
     7501 aatccatcaa tgtacgtaac ccttcatcga cgatcaagat gacatcgccg ccacgaccac
     7561 cgtctccgcc agccggtccg ccgtcaggaa cgtatttttc gcgacgaaat gcaaccattc
     7621 cgtctccgcc cttgccagct ttcacatcga tcgttacttg atctaaaaac atggacataa
     7681 ttaagtcctc ctattattca tttatttatt tgactgcctg aatttccaca ataaacaaaa
     7741 gtcttactta ttttatcggt gaaatctgat tttgtctatc atttcagcaa aaagattgtt
     7801 taccttcttc tttttttatc atttggtaag gtttccaacc ttttttctgc taccaagtat
     7861 aattttttga caaaatattc tttgcgtagc agaataacag tgtattaatt gtattaattg
     7921 aggatgccgt ttcacagcac ccctaataaa ggaggaactt ttgatatgac agtaaaagta
     7981 ggtattaacg gatttgggcg tattggccgt ctggctttcc gccgcatcaa agaagtctca
     8041 gatgatattg aagtagtagc aatcaacgac ttaacaagtc caactatgtt agcacaatta
     8101 ttgcaatttg actctactca tggtacttat cctggcacag ttactgcaac agaagactca
     8161 atcgtagtgg atggcgaagc aactcgtgtt tatgcagaac ccgacgcaag caaaatccct
     8221 tgggcaaaag aaaacggtgt agatatcgta ctagaatgta ctggttttta tacatctgaa
     8281 gaaaaagcaa aagcgcattt agatgctggt gtcaaacgag tagttatttc tgccccagct
     8341 ggtgctatga aaaccatcgt ctataacgtc aatgatgata cattagatga aaacgacaaa
     8401 atcatctcag caggttcatg tacaacaaac tgtttagcac ctatggctta tttcttaaat
     8461 aatgaatttg ggatcgaagt tggtacaatg acaacagttc atgcctacac ttccacacaa
     8521 atgctcttag atggacctgt aaaaggcggt aatttgcgtg ctgctcgttc agctgctgac
     8581 aatactatcc cacattctac tggtgctgcg aaagcaatcg gcttagttat ccctgagcta
     8641 caaggaaaat tacaaggaca cgcacaacgt gttccagtcg ttgatggttc attaactgaa
     8701 ttggtttcag tattgaaaac aaaagtaact gctgatcaag taaacgaagc aatcaaaaaa
     8761 cacacagttg acaatccttc attcgggtat gatgaccgtc aaatcgtttc tagcgatgtc
     8821 attggaacaa ctcaaggttc gatctttgac ccaacgcaaa cagaagtaac aactgctggt
     8881 gatttccaac ttgttaaaac tgttgcatgg tatgacaatg agtatggatt tacttgccaa
     8941 atgattcgct tactagaaaa attcgctaac ttgtaatttg atagatcaat cgataaaaga
     9001 agcctttgac tggatccagt caaaggcttc tttcttttca tcccctactt ttaaactcgt
     9061 tcaatgaaca tattggctta aaaattgttg gactacttgc tgatattttt caggatctgt
     9121 cttgtatgct tccgcatgct cggctccttt tacaacatac ttttcttttg gtcccttcgt
     9181 tgcacgatag accttatcta acatttcaaa tggtacaaat gtatcttcgt ctccatgtat
     9241 aaagagcatt gggcgctggt ttttcttcaa ttgttccact gcatttgctt caccgaaaaa
     9301 gtagccagca cggacttttg tcactagact cgttacttgc ataagcggaa atgctggaag
     9361 actaaacata tcgtctagct gataagcaag ctcatcatga acggaagaat aaccgcaatc
     9421 ttcaatgatt gctttcacat tatctggtag tttttctccg ctagtcatca taactgttgc
     9481 tgctcccatg ctgacaccgt atagaacgat ttcttgcgat tttccattgt tttctagcac
     9541 tttgtttatc cactgtacat agtctttacg ctctggccat ccaaatccaa tgtaatcccc
     9601 ttcgcttttt ccatgtcctc ttgcatcagg aacaaggaca ttgtaaccca tatcatgaaa
     9661 catctttgcg taattggtca tcgtttcagc gtttcccata tagccatgag ccacaagagc
     9721 tgttttttca cttttcgttt cagcaggaag ataaatcgct gataatttca gaccgtcttt
     9781 tgaagtgatg gtccaatcgc ttcggttttc ttttgaacta aaccatttat gtgcatccgt
     9841 ttctttggat gtctgtgttg taccaggtgt atcttcgtct aaaaagtctt tctcagaagg
     9901 cacaacagca taattataaa aataattccc tgcaaacact aacgcaacaa tcagagctaa
     9961 agcgacgcct aaaaggattt tcatccaaag tttcataaat catccatctc atttctcttt
    10021 tttcatataa ccttgaatta gtataatcaa agaatctcta ctatgcaatg ttcattttta
    10081 taac
//
LOCUS       DACECY010000084         9964 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.089, whole genome shotgun sequence.
ACCESSION   DACECY010000084 DACECY010000000
VERSION     DACECY010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.089"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            8..81
                     /locus_tag="IWO38_002429"
     tRNA            8..81
                     /locus_tag="IWO38_002429"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="IWO38_002430"
     tRNA            101..173
                     /locus_tag="IWO38_002430"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="IWO38_002431"
     tRNA            201..274
                     /locus_tag="IWO38_002431"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="IWO38_002432"
     tRNA            288..361
                     /locus_tag="IWO38_002432"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="IWO38_002433"
     tRNA            385..474
                     /locus_tag="IWO38_002433"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="IWO38_002434"
     tRNA            487..560
                     /locus_tag="IWO38_002434"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="IWO38_002435"
     tRNA            579..654
                     /locus_tag="IWO38_002435"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="IWO38_002436"
     tRNA            656..726
                     /locus_tag="IWO38_002436"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="IWO38_002437"
     tRNA            740..813
                     /locus_tag="IWO38_002437"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="IWO38_002438"
     tRNA            838..925
                     /locus_tag="IWO38_002438"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="IWO38_002439"
     tRNA            947..1018
                     /locus_tag="IWO38_002439"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="IWO38_002440"
     tRNA            1041..1130
                     /locus_tag="IWO38_002440"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="IWO38_002441"
     tRNA            1143..1216
                     /locus_tag="IWO38_002441"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="IWO38_002442"
     tRNA            1235..1307
                     /locus_tag="IWO38_002442"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="IWO38_002443"
     tRNA            1311..1383
                     /locus_tag="IWO38_002443"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="IWO38_002444"
     tRNA            1388..1458
                     /locus_tag="IWO38_002444"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="IWO38_002445"
     tRNA            1471..1544
                     /locus_tag="IWO38_002445"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="IWO38_002446"
     tRNA            1556..1627
                     /locus_tag="IWO38_002446"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     gene            1893..3086
                     /locus_tag="IWO38_002447"
     CDS             1893..3086
                     /locus_tag="IWO38_002447"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ0314480.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="IWO38_002448"
     CDS             3216..4688
                     /locus_tag="IWO38_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0314481.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="IWO38_002449"
     CDS             4771..5559
                     /locus_tag="IWO38_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ0314482.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="IWO38_002450"
     CDS             complement(5626..6294)
                     /locus_tag="IWO38_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ0314483.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="IWO38_002451"
     CDS             6409..8121
                     /locus_tag="IWO38_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0314484.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9896
                     /locus_tag="IWO38_002452"
     CDS             8124..9896
                     /locus_tag="IWO38_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0314485.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2900 a         1781 c         2278 g         3005 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa agttaaatta
     9901 aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt ttttataagg
     9961 ggca
//
LOCUS       DACECY010000085         9924 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.090, whole genome shotgun sequence.
ACCESSION   DACECY010000085 DACECY010000000
VERSION     DACECY010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.090"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..357
                     /locus_tag="IWO38_002453"
     CDS             1..357
                     /locus_tag="IWO38_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314486.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            373..723
                     /locus_tag="IWO38_002454"
     CDS             373..723
                     /locus_tag="IWO38_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HAQ0314487.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            752..1531
                     /locus_tag="IWO38_002455"
     CDS             752..1531
                     /locus_tag="IWO38_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HAQ0314488.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1542..2219
                     /locus_tag="IWO38_002456"
     CDS             1542..2219
                     /locus_tag="IWO38_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HAQ0314489.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            2238..3344
                     /locus_tag="IWO38_002457"
     CDS             2238..3344
                     /locus_tag="IWO38_002457"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HAQ0314490.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3334..4425
                     /locus_tag="IWO38_002458"
     CDS             3334..4425
                     /locus_tag="IWO38_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HAQ0314491.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4438..5178
                     /locus_tag="IWO38_002459"
     CDS             4438..5178
                     /locus_tag="IWO38_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HAQ0314492.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            5466..7406
                     /locus_tag="IWO38_002460"
     CDS             5466..7406
                     /locus_tag="IWO38_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0314493.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            7493..9415
                     /locus_tag="IWO38_002461"
     CDS             7493..9415
                     /locus_tag="IWO38_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAQ0314494.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(9695..>9924)
                     /locus_tag="IWO38_002462"
     CDS             complement(9695..>9924)
                     /locus_tag="IWO38_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ0314495.1"
                     /translation="IDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDLKDPAQAV
                     GSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT         3236 a         1647 c         2205 g         2836 t
ORIGIN      
        1 atgattaaag tagttattgc agaccgtatg ggtaaaggac aaaatgtggc caaaggggta
       61 gaagcagctg gtggaaaagc agttgtcgta ccaggaatgg gggcagatat gcgtttgggc
      121 gatgtgatgc aacaagagca tgcagatatg ggtatctctt tttgtggtag tggtggtgca
      181 ggtgcattaa cagcagcaac aaaatatggc tatccagaac gacatggtat gcgttcgatt
      241 gaagaaggga tcacagctat caaggatggc aaaactgttc taggtttcgg ctttatggat
      301 caagaggaat tagggaaacg tctgacagaa gaatttatca aaaagcatgg aaaatagaaa
      361 gagggacatg aaatgagcgt tcaaaagaca attcagcaaa aagttatcgt ctccggtaaa
      421 ggggaaacaa agcaacatgc ttttgcatca gcgttagcag aaattcaaaa caaaataatg
      481 gaacaatcaa acgtattatt gcgaatcgaa ccagtcaatg tggatatttt aaaggcggtc
      541 gaaaacaaat acacagaacg ctttctattt ttcttcttcc cacggcaacg tattagctat
      601 gaagtcgaac tctctgttac tgtggctgta acagaaataa aattggcaga catatcgttc
      661 agccagcaaa tgactgaaga tccaaatggg attgccattc catttgtaac taaaaaaatc
      721 taattttgaa tcataagggg gaaagaataa cgtggatttc gtaatcattc tagtcaaatc
      781 ggtcattatc gggggattac ttggttttgc agcatctgtc ggggcagctc gaatgtttca
      841 tgcacctaat acacaaggtt taggagcttt tagaacatta ggcgaaatga atgcctgcat
      901 gggtgatcca gcttctcatt tttcatttgg tttaggcttt ttctttaatg catgggcttc
      961 ttcagttggt gctggtgcat tcacacaaga tgtgacgcat cgaatcattc ccaattgggc
     1021 agcggcagca cttctagcaa aaaacaaaga tgtcacccag accatgcatg atccgaaaaa
     1081 aatggggatc attggagcgg ttatcggggc agtggtggta gccttcttaa acacaacagc
     1141 tgcagcaatt ccagaggcgt tacaagtgac ggcggtttcc gttttggtac cagcagcgac
     1201 gattctgatc aatacagtaa tgccagttat tttttggttg gcagctttag acgcagggaa
     1261 acgaactggt ttttggggca ctctttttgg tggtatcgcg caattgatta tgggaaatgc
     1321 ggtacctggt gttgttctag gaatcttgat tggtaaaggc gtcgatgaga gcggttggaa
     1381 tcgcgtcacg aagagcatga tgatcgctat tgttttatta tttattttca gtggattttt
     1441 ccgtggattt gatatggaaa tgatcaagag cttccagctt caagtgccta attggcttga
     1501 taacttgcac aacatcttta gtgtgaaata ggaggagaat catggagaca acagaagaac
     1561 aagcaaaagt attagaagca aagcggcaaa agagcttttg gtttgcagac tggtcttttc
     1621 cgctaatcgt aggaattatg tccgctgctg tatttgcagg aacacacatg tatgtgatgt
     1681 atggtgtggg tgcattcaat gaagtttcga tcgtggcgat gttaaaagcc gggctggatg
     1741 gcggatccta tggcgcagcg gcagcgtttg gtgctagttt cttatttgcc agaattttag
     1801 aaggctcttt agttgggatc ttggacttag gcggttcgat cctaactggg atcggaattg
     1861 gggtaccagc aattctctta agtatgaaaa tagcggcacc aatcgataat tttgcactct
     1921 ctttattgac tggtttggtt ctaggtttga ttgttggtgg catcattgtc ttgattcgta
     1981 aatttacgat caatcaatca aattccactt ttggtgctga cgtcatgatg ggggcaggaa
     2041 attcatctgg acgttttttg ggtccattaa ttatattaag cgcgtgtgca gcatctattc
     2101 caatcggcat tggcgctaca ttaggagcac taggattcta tgcttggaaa aaaccaattg
     2161 ctggaggtgc tattttagga gcaatgattt taggagcaat cttcccggca gtaaattaat
     2221 agaaagaggg tcattaaatg tttgatactt tgattaaaaa tggaaggtta gttgatggat
     2281 ctaaaataga ggtcgctata gaaaaaggca tgattaaagc agttgcagca gaaataaatc
     2341 agccggcgca agaagtagtt gacttggagg gaaagtatta tttatccgct gggtggatcg
     2401 atgatcatgt ccactgctat gaaaagatga atctttatta tgactatcct gatcagatag
     2461 gggtcgaaaa aggtgtgacg acggtcattg atgctggaac tacgggagca gaaaacatcc
     2521 gtgaatttta tcaattgacg aaatcggtca agacaaatgt gtatgcgcta atgaatatct
     2581 ctaaatgggg cattgtcaaa caagatgaac ttgctgatct aacaaagatt caagaagatc
     2641 tagtaagtac tgcgttagca gaactgccgg aatttattgt cgggattaaa gcgagaatga
     2701 gtaagacagt tgttggtgaa aatggcatta agccattaga attagctaaa aaaatccaga
     2761 cagaaaacaa tgatctgcca ttgatggttc atatcggttc tgcacctcct gagttagaag
     2821 aagtattgtc aagaatggaa agaggcgatg tattgaccca ttgttttaat ggaaaaccaa
     2881 acggtatttt agatccaaag acagatcaaa ttaaaaactt tgtctgggat gcttacaata
     2941 atggtgtcgt ctttgatatt ggacatggga cggatagctt taatttccat gtggcagaaa
     3001 ccgcactaaa agcaggaatg aaagctacct ctattagtac ggatatctac atccgcaatc
     3061 gtacaaatgg cccagttttt gatctagcaa ctactttaga aaaattgagg gtagtcggct
     3121 attcttggga agaaatcatt gaaaaagtta cagcagttcc tgcacggaat tttcatttga
     3181 agaataaggg ccagttagct gtcaattatg atgcagattt gacgatattt gagattgttg
     3241 aaaatgaaaa ggaattgaaa gattccaacg gatttacacg tatagctaaa gaacaaattt
     3301 taccgattaa aacaattatt ggaggcgtca tatatgacaa ttagctatgg aaagtttgat
     3361 ttaaaagaag tcattaatgc atcgggaaaa atgacgatcc taggggtttc taaggtatct
     3421 gaaagtgtcc tagcagcaca acgatttgga ggcgagcatt tttttgagat gagtgatctt
     3481 gccatcaaaa ccggcgctta tctttctcaa ttactgaaag tggaagatgc gcaaatcgtt
     3541 tcttctgctt cagcagggat tgctcaatct gttgctgctt taatcggaca gggcgacatg
     3601 taccatgtct atcatcccta tacagaaaaa atcgctagac gggaaatcat catacctaag
     3661 ggacataatg tagattatgg gacaccggtt gaagtaatgg ttgctcaagg tggaggaaaa
     3721 gtcatagaag ctggctatgc aaatatgtgt acaccggaac atgtttccat gatgattacg
     3781 gaaaaaactg ctgcgcttct atatattaaa agtcatcatg cagttcagaa aagtatgctg
     3841 acggtggcag aaatggtaga agttgccaaa gctcatcgtt tgccattgat cgttgatgca
     3901 gctgcggaag aagatttatt caaatatagc gaaatgggcg ttgacttagt gatttactct
     3961 ggggcaaaag caatcgaagg tccgagtgcc ggactggtca taggaaaaaa gaagtatatt
     4021 caatggattc ggctacagtc taaaggtatt ggccgagcaa tgaaaatcgg taaagataat
     4081 attttaggct ttacccaggc actagaagat tatttaaaaa atgggagcga atccggtgac
     4141 tccatgaagc aacgattagc gccttttgtt aacgcaatca atcaacttcc tgacctttca
     4201 gcgaaaatag ttcaagatgg tgccggcaga gatatttatc gagcaagtgt aacgattaat
     4261 ggcgataaaa gtgcgaaaga agtcattcag gaattaaaag cacgaaatcc agcagtctac
     4321 acccgagaat atcaagcaaa taacggaatc attgagttcg acattcgttc cgtcagccaa
     4381 aacgaaatgg aaaaaattat aaaaagattg agagaaatca tgtaggaggc ttttaaattg
     4441 tcagttatcc caaattatta taaaaatcgt gtatgtttaa atgtattagc tcattcagtt
     4501 gaaaatgcaa aagcatgcta tgaagcagcg gaagggcatg tcgtactagg tgtgctatcg
     4561 aaaaattatg ctagcgatga agcagcaatc gaagatatga aaaggtacca agacgcaacc
     4621 aataatgcgt tgtcagttgg attaggcgct ggagatccaa accaaagtca gatggtttcg
     4681 cgtttatctg gggttttaca accacaacat gtcaatcaag tatttactgg cgtggggact
     4741 tctcgtgctt tattgggcca aaatgataca gtcatcaatg gattggtttc accaacaggt
     4801 aagatcggct atgtcaatat tgctacaggt ccgttaagtt cacaagcgcc agcagctgaa
     4861 gtgcctattg aaactgccat tcaaatgctg caagacatgg gaggtacttc aatcaagtat
     4921 ttcccgatga aaggcttagc acatagggaa gaatttaaag cagttgcgga agcttgcgca
     4981 aaatatgaat tctatttaga gccaacagga ggaattgatc tttcaaactt tgaagaaatt
     5041 ctgaaaattg caatcgatgc aggtgtaaaa aaagtgattc ctcatgttta tagttcaatt
     5101 atagataaag aaacaggaga tacgcgtata ggagacgtaa agatactatt aagcatggta
     5161 aaaaaaatta ttcactaact cattagaaaa taagaagctt attccagaat gaaattaaat
     5221 ggaataagct ttttataatt aattacggaa attgttaatg tagataataa tttagatgta
     5281 cgtaagcaga agtacagaaa tgtaagattt tccaatatta ttttattcaa cataataaat
     5341 cgtatatcaa tttttttagc gaaatttcta tttattccaa gaatttcagt tattacggaa
     5401 tcattttgtg cttaataaaa agaaaccgtt tttatatact ataaatgtag aaaggcggta
     5461 ggaaaatgaa aaaaattatt gcaatcacat cctgtccagt tggtattgcc catacataca
     5521 tggcagcaga aaacttagag aaagcgggga aagcagttgg cgccgaggta aaagtcgaaa
     5581 cacatggttc catcggtatt gaaaatgaat tgactgcaag agatatcgaa gaagcagctg
     5641 gtgtaatcat cgcagcagat actaaaatag ataaatcacg attcggcggt aaacctttga
     5701 taaccgtagg tgtgcaagaa ggaatccacc atgcagatga gttggtgaga gatattttag
     5761 caggaaaagc gccagtctat aaaagtacgg aggcaattat ttcttccgaa aataaaagtg
     5821 aatcagaaaa tttaggtaaa aaaatctata agagtttgat gaatggcgtt tcgtacatgg
     5881 tacctttcgt agttacagga gggttattga tcgcgatttc tttaacatta ggaggaacag
     5941 ctacacctga agggatcaag ataccagaag gaacgatttg ggcaacgatg aatagtatcg
     6001 gaagtatcgc tatggggttg atggttccaa ttctatctgc atttattgcg caaagtattg
     6061 cagatcgtcc aggacttgtt cctggatttg ttggtgggat gttagcggca aacggagcat
     6121 tatatggtag tgatgcgaat gcgggattct taggcgggat catcactgga tttttagcag
     6181 gattcattgc tttaggaatc aagaaagtgc gagtaccgaa agctttacaa tcgatcatgc
     6241 caattattgt tatcccaatt ttaggatcat tggcagttgg ttttgtgttt atctatgtta
     6301 ttggagaacc agtagctctt ctatttagtt cattgactca ctttttagca gggatgcaag
     6361 gaggaagtga aattgttcta gctatgatct taggagcgat gattgctttt gatatggggg
     6421 ggccgttcaa taaagtagcc tttttgttcg gatctggctt gattgcagaa ggcaattatt
     6481 ccattatggg gccaattgct gtagctatct gtatccctcc attagcattg gggctggcat
     6541 cattgatttt aaaaaccaaa tttacgaaaa cagaacgaga agctggtaag gcgtccttag
     6601 caatgggact gtttgggatt acagaaggcg ctattccgtt tgcttcaaca gatccactac
     6661 gggtgattcc aagcattgta atcggggcaa tgacaggaag tgtgatcgcg atgctttcag
     6721 gagtgacaga tcatgtagct catggcggtc cgatcgtagc ggttttagga gcagtggatc
     6781 acgtagccat gttttttgta gcagtgattg ctggtattgc tgtaaccgtg ctaatgttaa
     6841 gaatccttaa accgacatta caacaaactg cggttgctgc agcaaatggt gtgacagtag
     6901 cagatctatc agagactgaa acaagtgatg tttatacgaa aactgaaact tttgattcta
     6961 tcacggatat tatgtcagat gaagcaatcg ttttaggtga aacagcaaca acaaaagacg
     7021 gagtaatcga agatttagtc caacgtttag aaaaaacagc ggcgatcaca gataaaacca
     7081 gatttaaaga agtcatctat gatagagaac atgaaagtac aacgggaatc ggtatgggga
     7141 ttgctattcc acatggaaaa tcagcttatg ttcttaaacc aacagtggca tttgccagaa
     7201 gtcaatctgg ggtagattgg cattctttag atggaaaacc tgcacatatg atctttatga
     7261 ttgctgtacc tgaaaacagc caaggtgata tgcacttgaa aatactgcaa cgtctatcac
     7321 gtaaattgat ggatgatgac ttccgtcaag cactgatgga agcgccagat aaaacaacag
     7381 ttcaccaatt attaagcgaa atgtaacata agatgaaaaa tcgaccgtga tgagacataa
     7441 tagatgtgtt atcatgatcg attttttatt tagaagggag tttctgcaca ttatgaatca
     7501 gcgtcagcaa tctttgatct atcaattaag tgagaacaat aattatataa caacaaaaaa
     7561 gctagcagag catttccatg tgtcagaaag aacgattcat agcgatttga ataccattga
     7621 aacttggttg agagacaaac atctgccttt aatgtttgaa agaaaaaaag gtacaggtat
     7681 cttgttgaat ggtctgcctc acgaaaggga acaactgaaa cgagcgctta atgaagagac
     7741 tcaaacagaa atggataaaa aacagcttcg tcagttattg attttccatc tgctagtagc
     7801 aaaagacgga tttagtcttg aagaactttc agaaaaacta tatgttggaa aacgtacgat
     7861 tcgtaaagaa ttgacagagt tgcaaagctt ttttgagtat caccatttac agctagtatc
     7921 caaaacaaaa ctaggaactt ttcttaaagg cgatgaacaa gaaaaaagac agttgcttgt
     7981 taagacatta cgaaatatgc agaaagaaga tccacaaagt ccgtcgttga aagaattctt
     8041 cgctaaagat acattaaaag ttatccaaca tacattacaa gatgtttttt atgaaaacca
     8101 tttagaacag ccgaatggac tggctagtgt ggagattcac atctacttta tgctcgagcg
     8161 aatgaaacag taccagaaag tgaaattaag taaagacgaa aacgaagtcg tggaacacac
     8221 ccaagcacaa aagttaagta gtcaaatatt agcaaaacta gccaccatct atccaattga
     8281 attttctcct gatgaaatca attatctggc tttgcgtatc gccaatcttt ttacaaattc
     8341 acaagcagcc acacgttttc aaaaggaatc tagcgcactt gctgatcatt tgatcgaaca
     8401 agtggaacag tttctaggct attcattaaa agaagatcag ctactaaaac aaaatttaag
     8461 gtctcattta tcttcaactt attttcgact ccattatggt ttgcagattt ctaatccatt
     8521 gacgaaaaat gtgtttagta cgtacacaca acttttctta gttttacagt taattttaga
     8581 agattatttt gaaaaggaaa atttttatgt tccacaagag gagacagctt atttaacgat
     8641 ccatttccaa gcagcgatcg agcgacacaa acatcagaaa agtagaagat atcaaacggt
     8701 tttagttagt gaatattcaa aagctatggc tacttttctc gaagcacggc tgaacagaga
     8761 acttccagaa ttaacgatta tggatctagt tgaatataag ccagaattaa agagacaaga
     8821 ttttccctct gttgatttta tcttggctac gttaccattt aaacatataa gcattccagt
     8881 aatcgagatc tcaccaatga tcacagaaac tgatctagcc tatttgacaa aatatatgct
     8941 ggagcatgtg cctatcaaga agaaaaaaac atttgatcta gcaagcttta ctcatccatt
     9001 tctgatcttt cctcaacttg aacggacaga cccagccgat attttaaact ttatgggaaa
     9061 cgtcttagtg gaacatcatt atgtggagcc ggaattcatc gattctgtct tggaaagaga
     9121 cagtcactcc agtactagag tagcaccatt tgtaacaatc cctcacggta atccgctgta
     9181 tgtgaagcat tcgatgattt ctattgctac aatgaaagaa ccagtcctat ggcacgggga
     9241 acagatacgt atcgtactgc tattagcaat aaagaaaaat gatttgaaac atgcagagtt
     9301 caaaaaaata ttttctgtta ttcactatct agaaaagcag ccagaacaaa tggctcagtt
     9361 gatgcaatcc aatcatccgc tagaaatctt aacgttgtta tctagttatg aataagtagc
     9421 gattaaaaaa ctgcctttat atttataata attctatcta tttatttgga agtagaaaac
     9481 taaatacaaa aaaagaggat gtggccaaag ttgtggcaca tccttttatt ttgagtttct
     9541 tataattttt tatagttgct tacgatgtct tgactactgg taaacggtgt tcaagagaca
     9601 atttgtcggc aataaggcac aattttgtaa aatatggaaa gctatttttt taacattgtt
     9661 tcttagtgct tgaaaaatca ttttgaatac ttctttatac gatcgatgta cgttgattta
     9721 agtctaaaac ttttcttttg attccttctc tgacttttct gaattccgtt aatatttctt
     9781 cttcgctccc gacagcttgt gcaggatctt ttaggtccca gtgaaggtgt tcgtgattgt
     9841 ttggaacggc agggcacttg tctttggcat caccacaaag tgtaatgatc aagtctgcct
     9901 gagtaaagta atctctatca atca
//
LOCUS       DACECY010000086         9808 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.091, whole genome shotgun sequence.
ACCESSION   DACECY010000086 DACECY010000000
VERSION     DACECY010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.091"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(32..2152)
                     /locus_tag="IWO38_002463"
     CDS             complement(32..2152)
                     /locus_tag="IWO38_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0314496.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2382..3560
                     /locus_tag="IWO38_002464"
     CDS             2382..3560
                     /locus_tag="IWO38_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ0314497.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3576..4334
                     /gene="aroD"
                     /locus_tag="IWO38_002465"
     CDS             3576..4334
                     /gene="aroD"
                     /locus_tag="IWO38_002465"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ0314498.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4357..4842
                     /gene="ybaK"
                     /locus_tag="IWO38_002466"
     CDS             4357..4842
                     /gene="ybaK"
                     /locus_tag="IWO38_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ0314499.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4913..6166)
                     /locus_tag="IWO38_002467"
     CDS             complement(4913..6166)
                     /locus_tag="IWO38_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0314500.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6283..7632
                     /gene="gdhA"
                     /locus_tag="IWO38_002468"
     CDS             6283..7632
                     /gene="gdhA"
                     /locus_tag="IWO38_002468"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ0314501.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7744..9090
                     /locus_tag="IWO38_002469"
     CDS             7744..9090
                     /locus_tag="IWO38_002469"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ0314502.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9398..9784)
                     /locus_tag="IWO38_002470"
     CDS             complement(9398..9784)
                     /locus_tag="IWO38_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0314503.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3126 a         1782 c         2010 g         2890 t
ORIGIN      
        1 ttttgtcgct gctcctttta aatttatttt atcattcgga agaacttgtg gtcgttccgt
       61 gaagttcttg gtacgtttgc gttttataaa gatcaacagt tgattgtccg ttgtgttcac
      121 taaagacact agttgacttg tcaccaagag atttttcagc ttcttttaat ttctctaagc
      181 cattcttgta gtcataatca ctggtatcaa ttgggtctaa tccactattc gtatagaacc
      241 gaagaagatc accattattg atttggtcag aaacagctaa ctgtttatca actttttctt
      301 tcagtgaatc aatttcattc tgtacttcct cagtaggttc agtaatagct tcaccagttt
      361 cgttactata gatcgtacca ccgtagtaag tatattttgg tgtaacaaaa tcaccatcac
      421 ggaaagaaac aagctgttca tgatctttcg agaatagatc ttgtcctaac tgaatataat
      481 tttttgtatc tacacctagt aagtgaagta atgtaggcaa ggcatcaact tgtccgccgt
      541 aagtgtgatt gattccgccc tgtgtttgtc cagggatatg gatcatataa gggacacgct
      601 gcatttgtgc attgtcgtag tttgtccaag tagaacttgt ttttccaact aattcagcta
      661 gatttttatt tcttgaattc gagacaccgt aatggtcacc gtaaaggacg atgactgaat
      721 tatcatacaa gccgctttct ttcaaataat tgaagaattc ttgtacggcg gtatctaaat
      781 aattggctgt cgcaaagtaa ccgttgatcg tttcatcatt tgtattggct aacgggaatc
      841 ctgcttcgtc attggtgaat tttgaataag gataatggtt agatacagca atgaattttg
      901 aataaaatgg ttgttgcaga cgttccaagt attgtacgga ttgttggaaa aatggtttat
      961 catgcaaccc gtattggaac gaattgtcat cattaacatc ataataagaa gaatcaaaga
     1021 aataatcata tcctaaattc ttgtatgtct cgtttcgatt ccagaatgta ccagcgttcc
     1081 catggaatac cgcactaatg taaccttgtg tttgacttaa aatatcaggt gcagcttgga
     1141 aagtattttt tccgcctaat tgtgtgaaca aggagccttg attcaagcca aacaatgaat
     1201 tttccatcag cgtttccgca tcgcttgttt ttcctgcttt tacttgatgg aaaaagttgt
     1261 caaaactgaa cgtgctgttg ctgtggtaaa gactattgat gaaaggtgta acttcatgct
     1321 ggagaccatt ctcatctgtc aatttataat cgatcaggaa ttgctggaag ctttctaaat
     1381 ggatataaat cacatttttt cctctagcga gcccgaacaa atcgtcattt ggtgctgcat
     1441 aatgttcttt tacataattt tctacttctt tcatatcatt gggacttgct tctgcccgca
     1501 cttggtttgt ctggtaagtt tgaacaccgt cataaaccat gaaagcattc aagccaagat
     1561 atttgaccaa ataatcacga gagaatgttc gagtcagcaa ttctggacga tcagcttccg
     1621 ccataaaaag atttcctgag aaaatcataa ctgctaatgt agagatcgca aaagccattc
     1681 ttgctcggat cgggcgaggg tccaacttga ttttttttgc aatcagcaga acagtcaaga
     1741 taataaaatc gatccaatag atcacatcat atgggcgaaa cagtcgaatg gcactttctc
     1801 ccagaccact cgctactttc ccagcaccta aaatcgtgtt gatcgtaatg aagtccgtga
     1861 attctcgata ataagtgaca ttcgaaaaaa caagcaatgt caaaagaaaa taaatgacca
     1921 tcatcgtgat ataagcaact ctagggcgtc tgatataaag agcaattcca agtaataaca
     1981 atgtagttgc aatagggttg ataaacaaga taaagtattg aatgccactt tcgattccga
     2041 gatggaaatc aactaaatag gcaaaaatcg ttttcaacca aagtaaaacg actagtaaag
     2101 aaaaaaatcc gagcctctta ttcaaaaagg caggtgtttt tatacatttc aagataatct
     2161 tcctttctgc acttcttaat cacagaagtg tcctgaaacc atgttacaca gctcacttat
     2221 tctcatttat tttactagta tgacaatata gtgtcaattg aaaccagaag tttatgctgt
     2281 ttcattaagg ttttctttaa gcttcatgaa aaaatgagca gtaagacaag tcctatgttt
     2341 tttgttatac ttgggaagag aaagctaaag caggtgaagg aatgaaagta aaagtaaaaa
     2401 aaagagcaag caatcgattg aaaaaaggat atccactgat ccaaaaagaa gaccttgctc
     2461 atccccaaaa agaaaccacg caatgggtgg aattagttga ttcgcaaaac caatttatcg
     2521 caaaaggata tctaggaaag cagaataagg gaatcggttg ggtattgagc cagcagaaca
     2581 tcccatttga tcaatctttt tttgaagaat tttttgtgaa gtcaaaagaa aaaagaactt
     2641 tctttttttc agatgattct actaccgctt ttcgtatatt caatggagaa ggagacggga
     2701 tcggcggtct gattgttgat tattatgcag gttatgctgt tttttcttgg tataacgaaa
     2761 cgttatatgc acatcgtgaa ttattgattc aagcttttcg tgaaatctac ccggaaatca
     2821 taggaggcta tgagaaaatt cgatttgaat cagtaaatct gcctgagagc cagttcattt
     2881 acggaaaaga ggcaccggaa cccttgatcg taaaagaaaa cggagtaaaa tttgccgttt
     2941 atttaaatga aggattaatg actgggatct ttttagacca aaaagaagta cggggatcat
     3001 tagcagaagg tttcgcttct gggaaaacag ttttgaacat gtttagttat accggcgcct
     3061 tttctgttgc tgccgcaatg ggaggggcaa tcgaaacaac aagtgtcgat ctagcaaaac
     3121 gcagtttaaa aaaaacacag gaacagtttg aagtgaatgg tttggatgtc gatcagcaaa
     3181 aaatcattgt tatggacgta ttcgagtatt tcaaatacgc caaaagaaaa caatttttat
     3241 atgacatgat tgtattggat ccaccaagtt ttgccagaaa taagaaaaaa gtctttcgcg
     3301 tagcaaaaga ttatggggaa ctggtcaagg atagtctgga tattttagca gaagacggga
     3361 tacttattgc gtctacaaat gctgcgaatg tctcgataga acaattccaa acaatgattg
     3421 aagaggaatt ccatgccaaa aatgtttctt ttgaacaact tgctttatat cgtctgccaa
     3481 aagatttcca gacgacggat acctttttag aaggcaatta tctgaaagtg tttgtttatc
     3541 aaattaaaaa taaccattag aaggagcgat gcagaatggt tcgggtaaag ggattgaata
     3601 taggaagcgg tagaccaaag atttgtgcgc ctcttatcgg ggccgtccga gaggaagtat
     3661 tacgtgaagc agttcttgca cgtgaagcag gggccgattt agtcgaatgg cgagtcgatc
     3721 attatcgtga agttttttct tttgatgaag tagcagaaac attggttctt atccatgaga
     3781 tgcttgatgg actgccgtta ttatttacgt ttcgtacctt agctgaaggc ggagaacaag
     3841 acatatcgat ccgtcagtat cgcgaccttt atgaacatgt gatacacaca ggattagtag
     3901 atatggtcga tatcgagttg ttcaaagtag aaagtctagg aaaaagactg atagaggaaa
     3961 tcaagtcttt aaacattcct ttaattatca gtagtcatga ttttaaagaa acaccagcag
     4021 accctgtctt gctttaccga ttgaatgtta tggaacattt tggagccgat atcggaaaac
     4081 tggcagtaac tcccaataat gaacgagatg ttttgcggtt gatggagttg actagacggg
     4141 caaatgcttt tgtctccatg cctattatta cgatgtccat gggaaatctt ggtaaaattt
     4201 ctcgtctggt tggcggaacg acgggttccg ttatgacatt tggttcctta agcagcgatt
     4261 ctacttctgc tccaggacaa ttaccggtca aagcattgaa gcaaattatt caattattaa
     4321 atgaaccgtt gtagaacaag tttgaggtga tattttttgg caaagaaaaa aatagtaaaa
     4381 acgaatgcaa tccgtatggt cgaacaaaaa aagattccct atacagagca cgaatatgag
     4441 tgggacgaaa gccacctaag tgcgtcatcg gtcgctgaac agcttccaga aagccaatct
     4501 cgtatattta aaacactggt tgctgtgggc aatgtgacgg ggcctcttgt tgctgtgata
     4561 ccgggagaag ccgaattgaa tctaaaaaaa ttagcaaaag tttcagggaa taaaaaagta
     4621 gaaatgctcc atttaaaaga tttggaagca acgaccggat acatacgtgg tggctgctcg
     4681 cctataggta tgaaaaaact atttcctact tatttagatc aaattgcaga aagctatgaa
     4741 caaatcattg tctcggctgg cagaagaggg ctacagatgg agttagcacc tcaagatatc
     4801 tgtgcattaa cgagcggaca atttgcagat ataaaacaat aaatcatacg tataaaagaa
     4861 agaaggaggc cgggatgttg tttgtccagt ctccttcttt cttttttata cattatgctc
     4921 tttgcccggc attcattcct gctacatgcc ctgtagcgaa agccgctgtg atattaaagc
     4981 cgccagtata accattgaca tccagtaatt ctcccgcaaa atacagacca tcaatgattt
     5041 tactctccaa tgttttagga taaatttcct ttagagaaac ccctccgcca gttacaaatg
     5101 atttctcaat aggaaaagtt ttgttgattg atagcttaaa ctctttgcat agttgaacaa
     5161 aatcttgaat ttgtttttga gaggcttgtt gtcctgtcag atgatccatt tcaagccgct
     5221 caagaaagaa aactaacaat cgctcaggca atataccatg ccaagcattg accaagttct
     5281 ttttggttgc tttacttttt tctgtcaata tatgaataag ctcttgtttg gtttgtgtag
     5341 ggaaacaatc cagagaaagt gtcactggtt ctcccgtttt ctctaattct ttattgacaa
     5401 aacttgaaca acgcagtgca gccggaccag aaataccgaa atgggtgaaa agtaaatcca
     5461 tcgtatgttc agtcaataca cgaccttttt tatttaaaac gcgtaaagtg atgtcttgta
     5521 aagaaagccc ttgaagtgtt ttctcctgaa tatacggttc atccgatatc aagggggact
     5581 ctgtcgcata aagaggcgtc accgtgtgac ccactcgctt cacgatcttg tacccatctc
     5641 cagtagaacc agtagatgga taagtccgtc caccagttgt taagataatg gcaggagcat
     5701 ggaactcttc gaattccgtg cgtataccgt aaattttatg ttctttgtgg atcaatttct
     5761 ctactcgagt agagaataga agcgtcacat ctagttcttt cagacgattg agcaatgctt
     5821 cgataatcgt ttttgattta tttgttacag gaaacatccg tccatgatcc tcttctttta
     5881 aatgtacgcc ttgtgattca aagaagttca tgatatcgaa attattccat tgcgcaaacg
     5941 tactatataa aaactttcca tttccaggaa tatgtgcaat caaatcatca acaggacgat
     6001 tgttcgtaac attgcaccga ccaccacctg tcatcagtag tttttttcct gcttttttgt
     6061 ttttttcgat caagagtacg cgtgcgcctt gttcagctgc actgatcgct gccatcatcc
     6121 cgcttgttcc agcgccgact acgatcacat catacagttt ttccattccc tcacctcaag
     6181 gaaagtgtag catatctgaa gaaagaaaac agaaaaaaat gttttgagcc aaaagagaaa
     6241 atgagctaaa atgagggaag ataagcaaag gagcgtggaa cgatgacaaa cgcaacagaa
     6301 tatgttcaag caattcaaga aaagctacat caaaaggatc aagggcaaga agaattttta
     6361 caagcaatcg atgaattcat gcccacggtt atgtcttttt tggacactca tcccgaatat
     6421 atcgaaaaga atatcttaga acttttaacg gaaccagaaa gagttattca atttcgagtg
     6481 ccttggcaag atgatgaagg aaactggcga gtaaatagag ggtaccgtat ccaatacaac
     6541 tcagctatcg gcccttataa aggtggattg cgcttccatc ctagtgtcaa tctaagtatc
     6601 ttgaaatttt tggcttttga acagattttc aagaatagtt taaccggtct gccaatcggt
     6661 ggaggaaaag gcggaagtga cttcgatcct aaaggaaaat ctgataatga gatcatgcgt
     6721 ttttgtcaaa gtttcatgct ggaattgtca aaacatatcg gcccatcgat tgatgttcct
     6781 gcgggagata tcggtgtagg agctagagaa atcggctatt tgttcggcga atataaacga
     6841 ttgaaaaaat atgatactgg tgtgttgaca ggtaaacctt tggatttttg gggaagtaag
     6901 atacgtacgg aagctactgg ttatggatta gtgtattatg tcaaacatct attgaacgaa
     6961 gaaaaagatt catttaatca aaaaacagtt tttgtttccg gaagcgggaa tgtagccatt
     7021 tatgcaatcg aaaaagtgca agagctaggc gggaaagttg ttacatgttc agattctagc
     7081 gggtacatct atgatccaga aggaatagat gtgaaactat tgaaagaagt caaagaaatc
     7141 aatagacaac gtttgacgga atacgcaaac caacgtcctt ctgctattta tcatgctgga
     7201 gaatctgttt ggacattgaa agaaaaagcg gatatcgctt tgccatgtgc tactcaaaat
     7261 gaaatagacg aaagcttggc cgaaatctta gtagaaaatg ggatcaaaat cgtagcagaa
     7321 ggcgctaata tgccatgtac agtaaaagct gtttctgttc ttcatgaagc aggcgtgtgg
     7381 tattgtccag gaaaagctgc taatgctgga ggagtagcag tatctgcttt agaaatgagt
     7441 cagaacgtcc agcgattgac ttgggaaaga gagcaagtgg atgagcagct ggatcaaatc
     7501 atggaaacga tttaccatac gtgtcgccaa acagcagccg agtacggcga cgaaaaaaat
     7561 ctgcttcttg gagcaaatgt tgctggattt gaaaaagttg ccaaagcaat ggcagcagaa
     7621 ggtttagttt gaaaaatgaa actttcttgg aaatattgtc agcattttgt ttatttgctt
     7681 gaaaaataat aaaaaaaaac gtaaagtatt acctgtagag taaacctata aggaggagaa
     7741 caaatggcac acattcattt tgactattca aaagtagcac cgtttgtcaa cgaacaagag
     7801 ttagggtaca tgcaaagcca agtaacagca gcccacaacg aattacggga aggtactggc
     7861 gcaggaagcg acttccgcgg ctggatcgat ttaccaacga actatgacaa agaagaattt
     7921 gcacgtatca aagaagcagc taaaaaaatc caatctgatt ctgaagtatt agttgttatc
     7981 ggtatcggtg gttcttattt aggcgctcgt gcagcgatcg acttcttgaa tcacacattc
     8041 ttcaatttat tagatagcaa cacaagaaaa gcaccacaaa tcttctttgc cggaaattca
     8101 atcagctcaa catatattgc tgacttgatc gaagtaatcg gtgatcgcga cttttcagta
     8161 aacgttattt caaaatctgg aacaactact gaaccagcga ttgcgttccg tgtattcaaa
     8221 gagcttcttg ttaagaaata tggacaagaa gaagcaaaca aacgtatcta cgctacaaca
     8281 gataaagcaa aaggtgcagt taaagtagaa gcagatgcag aaggttggga aactttcgtg
     8341 attcctgatg acatcggcgg acgtttctct gtattaactg ctgttggttt gttaccaatc
     8401 gctgttagtg gtgcagatat cgatgcattg atgcaaggtg ccgctgatgc aagcaaagca
     8461 tactcaagcg acaaactaga agaaaacgaa gcttaccaat acgcagcaat gcgcaacatt
     8521 ctttaccgta aaggcaaagt aactgagttg ttgatcaact atgaaccagg aatgcaatac
     8581 ttctcagaat ggtggaaaca attgtatggt gaatctgaag gaaaagacca aaaaggcatc
     8641 tatccttcaa gcgcaaactt ctcaactgat ttgcactctt taggacaatt catccaagaa
     8701 ggacgtcgca atatttttga aacagttgtg aaggttgaaa aacctcgtaa atcaatcaca
     8761 attcctgaac aagcagaaga tttagacggt ctaggctact tgcaaggaaa agaagtggac
     8821 ttcgtaaata caaaagcatt tgaaggaaca ttgcttgctc atacagacgg tgacgtgcct
     8881 aacttgctag tcaaaattcc agaaatggat gcttatacat tgggttatac aatgtacttc
     8941 tttgaaattg ctgtaggtat ttcaggttac ttgaatggaa tcaacccatt cgaccaacca
     9001 ggtgtagaag cttacaagaa aaacatgttc gcacttctag gcaaaccagg ctttgaagat
     9061 cttgctaaag aattgaacga acgcctgtaa gataagggtt tttcgctaga tttaaataat
     9121 cagtgataga aacatgttcg ctttaaaatg aaaactggta cccatttagg tactcaaaca
     9181 tactgcatcg tttattcaga ataaggggct gtgacaagac gtttgtcaca gtcccttgtt
     9241 tgtatagaaa tcgattgatt gccttgctct ttagtttgtt ttaggacttt aagaacagtg
     9301 aggctgactt cgaaggttaa ttctgttttt ttgttttcta gaatcagtaa gcaaaaaagc
     9361 actcacttcg agtgcttttt tgcgatatat tcttagctta agataggcct aatttttctt
     9421 ttacagatga gggtacctta tcgctggtaa ttactttttt ctctatgatc cgcttttcgc
     9481 ttgctttcaa ctgcaagatc gttccttcgg ggtaagcaga accattggca gagtcaccag
     9541 aagtaatgac atcgaaatct aactgtcttt tttcaccgtt ttcagcataa gcatctacgt
     9601 tgtatttaaa acctcttcca attacttccc cattatctgc tttgatatca gtttctgcag
     9661 gcattggagc tttgactacc gcataataat ctttacctac ataagtagac tgatagtagt
     9721 cccaaccttt ccatcctata aaagcaatca atatgataac gatcaaccct aaaatttttt
     9781 tcatgaaaga tctccttttt tattttta
//
LOCUS       DACECY010000087         9711 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.092, whole genome shotgun sequence.
ACCESSION   DACECY010000087 DACECY010000000
VERSION     DACECY010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.092"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            168..362
                     /locus_tag="IWO38_002471"
     CDS             168..362
                     /locus_tag="IWO38_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314504.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="IWO38_002472"
     CDS             631..930
                     /locus_tag="IWO38_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314505.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="IWO38_002473"
     CDS             complement(1027..1461)
                     /locus_tag="IWO38_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0314506.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="IWO38_002474"
     CDS             complement(1693..2403)
                     /locus_tag="IWO38_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ0314507.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="IWO38_002475"
     CDS             complement(2393..3253)
                     /locus_tag="IWO38_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ0314508.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="IWO38_002476"
     CDS             complement(3258..3896)
                     /locus_tag="IWO38_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ0314509.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="IWO38_002477"
     CDS             complement(3911..4210)
                     /locus_tag="IWO38_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0314510.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="IWO38_002478"
     CDS             complement(4253..5737)
                     /locus_tag="IWO38_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ0314511.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="IWO38_002479"
     CDS             complement(5757..6218)
                     /locus_tag="IWO38_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0314512.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IWO38_002480"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IWO38_002480"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ0314513.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="IWO38_002481"
     CDS             7521..8291
                     /locus_tag="IWO38_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0314514.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="IWO38_002482"
     CDS             complement(8564..9391)
                     /locus_tag="IWO38_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0314515.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         3035 a         1970 c         1510 g         3196 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgcccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatacttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt tttatgttct c
//
LOCUS       DACECY010000088         9344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.093, whole genome shotgun sequence.
ACCESSION   DACECY010000088 DACECY010000000
VERSION     DACECY010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.093"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(159..3800)
                     /locus_tag="IWO38_002483"
     CDS             complement(159..3800)
                     /locus_tag="IWO38_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314516.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVVYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMIDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGQDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEDDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTALKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKPSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            complement(3803..5752)
                     /locus_tag="IWO38_002484"
     CDS             complement(3803..5752)
                     /locus_tag="IWO38_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ0314517.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(5794..6852)
                     /locus_tag="IWO38_002485"
     CDS             complement(5794..6852)
                     /locus_tag="IWO38_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAQ0314518.1"
                     /translation="MKKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(6971..7426)
                     /locus_tag="IWO38_002486"
     CDS             complement(6971..7426)
                     /locus_tag="IWO38_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314519.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(8009..8812)
                     /locus_tag="IWO38_002487"
     CDS             complement(8009..8812)
                     /locus_tag="IWO38_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0314520.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         2712 a         1926 c         1339 g         3367 t
ORIGIN      
        1 cagcataagc cctttttaat tatacaactt tgaacagtaa acatttaatt catttataga
       61 caatttaaaa taattttttt gcataaatag aaattttcct tgacatcttc agttagaaat
      121 caaagtaaaa agaaggagca aatcaatgct ccttcttttt actcgtcttc agacttattt
      181 tcagttgatt tttgattacc gtattttaca atatagtcct tgactgacat ggtagatttc
      241 ttcactactt taccagtttt ggatgctcct gtagcagtag ccttggcaac cttagccgtt
      301 cctttaccta ttttaatagc ttcgttttta gcttcatagg ttatccctgc tagactacct
      361 tttggagctt ttgctaaagc tttatctgct gaggtgtaaa catttttacc agtttttgat
      421 agatattcaa cggctgattt agctgttgat ggtttcttgg ttgattgatt agatttccct
      481 gaattatctg aagtagattt agaattattt gttctattat ttttatttgt cagttcaggt
      541 tctctaattg attgattgtc tttcgacatt cctgatccaa tatttgtttc atatttatct
      601 gtttgattaa cattaggagt aggaatgaac tttttcttca catctgattg ttctttatcc
      661 gtttcttttg ttagcgattc tctgtcttta agtgctgtgt cattccgaat agcactatta
      721 tcaaagttgc tgttaagctc tcttgactga ctttcaccag atgctccatc tcctaattcc
      781 cgatgcactc tgtctgacac gtcctcacca ctcatttcat tcctcagttc ttgatgagtt
      841 tgatctaaag taatctcgtc atcttcattc tcaggaattg caacagcaat tggcgtatgc
      901 cgttgttgtt cgagttctct atttcgatct gccacaagcg catctctcgt ctcaatgata
      961 ttcccatcct catcgactaa atctccattc acattatatc catcgctgtg tttaccaagt
     1021 ttattaccaa actcatcaac aagatttcca tcttcatcaa ctaaacgacc tttttcgtca
     1081 actaaacgtc cgttactatc ctgaccaaaa tcaccattta gcaacctgcg tccattttcg
     1141 tcaacaagat ttccatcttc atcgactaag cgaccttttt catcaattaa acgtccttcg
     1201 ctatccatac caatattacc atcttcacta tcttccaatg gtttaccatt agtatctata
     1261 aggtttccat cttcatcgac caaacgccct ttttcgtcaa taaagttgcc tttagcatct
     1321 ttaccgtatt tactatcttt taatatttca ccattttcat cgacaaagtt tccgttttca
     1381 tcaatcatac gtcctaaatg atcaatatat tgttgggagt tattacgtag ttgatcttga
     1441 cgaactaaat ctttgaaaga ggtattattg attgttgaac caactccgcc accgttgaat
     1501 gacattcctt ctggtgtttc aggaattact ttatcagcaa tggacttagc atcagcattg
     1561 ctatctccca tattgttcaa acgttcagcc attttttgcg ctccaactgc tccaagacct
     1621 cctgcaacca tatcttttac tgagctgttg ccaccatcat aatagggatt tttagctcct
     1681 tggcttttct tgattgcttt agaaacgccg tccacaactc gcacagcatc atttctatta
     1741 ccaaataata acttagcacg ttcatcaaac ccaaacgcta cttcaagtgg taagccaata
     1801 agcatattaa tcaaattatt tactttttcg ccttttttaa agactaaaag atatcctaca
     1861 cctaaaatca tagcagatcg aacaatattt gctacgccat cgccaactaa tgtattagga
     1921 atgaatccca cagcagtatc accaaatgca cgaataaaag taatcagaaa gataggtaat
     1981 tgtagtccga tcagagatgt tgtaacaaca cctaatgaaa ttgccatcac acctgctcct
     2041 gatgcaagtg atcctgttag aactacttcc caaatccgaa taaattcacg tttaaatgct
     2101 ttcacgatat tcacattgaa catcgcccaa gcacatgcta agtaaagaac tgctaaaacg
     2161 attgattcag cgttcatcgc tagccacgag ccaaacttac ccatcgctcc atgaccaact
     2221 aaggatgctg tacgccattc tgtttttact gttgacatat tcttagcact gccttgaatt
     2281 cctgaaggag cgatattata cgcataaaac ttagctcctg atgtatcaag tgctgattgg
     2341 agcaataaag ctaccgactg tgtgctaaaa gttgcattgt tagcaacgcc ccaaggttga
     2401 gtagcatcaa agttatgcgc tgttcctttt ccatcaggct tgcgattgtc gtctgtcaca
     2461 ttttgatttg ctgaccaaat atatccattt aaattggttg gtttcttaat tgcacttcct
     2521 tcttttccat cccctaaatt atagcctgtc caaacagcag gcgctcttcc catataagca
     2581 tcattttcgg cgacattaac atcaccggca taagtattaa tattgaaagt atcattcgat
     2641 gaccattctt ttaatagagc cataccgact gaattagaat ctagatcatt attttgtctg
     2701 gcataactgc tagtattgat cgctcttatc attgcagatt ttttagatgg ttcataatct
     2761 gtatcaacga acttgatttc ggatgtaggc tttccgtcag gtacatcacc accagtatca
     2821 gatgatggag caagattttg agtagaagcc caaagtcttg tgttaatgat gtagtcagaa
     2881 acaacatttg ttggtatatc tccatctttt tttgctgact ggatgccgtt gatataaact
     2941 cccaatagcg atcctgccac tggtaagaat ataaacaata caaaaatacg aacaatccat
     3001 ttttcaagta ctggtgataa tcccttcgag ttaacaccat ctttcgacaa taatttcatt
     3061 ccttgataac ctagtattgc tgtgaaaaag agcatagata agcttgctat cgctttaaat
     3121 aattcattat ttaatcccaa attgttaagc aagctagtta atgctttaga taccggatta
     3181 gatacatccg atccaccttc tccccacccc ataagagaaa atatattcat actcatgaaa
     3241 aattttccaa ggagttcatt tatggattca gcaatattag aaaggattaa actaattgat
     3301 ccgataaaac taccaatcgt cctaccaata cttcctagaa tggatgatgc ttgactggca
     3361 atagcatttc ctgagactgt ttcaatatat ccgtagtaac tataagttgc taaagtagat
     3421 gctagttgag tacctgcatt accccctgca atcgaattac cttgtttaga aatgtcagaa
     3481 taagggaaat cggcactgat accaccttgt cctaacaaat tcatttgtcc agttttagcg
     3541 gcactatcat taacttcttt atctcccatt aggatacgag catatagtgt ccacattcct
     3601 tgttctgccg gtttactttc cgcatataca acgttcttat ttataactgt ttcttgtttg
     3661 ctatcttgat tgtcaggagt tggatcattc attccaaaag gagcgcctaa agtaactgct
     3721 aatactcctc caataactag acctcttacg acccatcgtt ttattctaag gttttgtagt
     3781 aaatatttct tgaatttcat agctacgccc ctccaatcga aaatacaatc ttgttttctt
     3841 tactttcagc aacggaaggc tttgcaataa tttcagcatt agaatcacct tttccttcaa
     3901 acgctccggc ttcactcaac ttaacatcga atatttttgc gtttggataa gtaattattg
     3961 cttcagtaat cgcttcttca aactcagatt caactaaatg caagatattc cttgcacccc
     4021 ctgatttaga atcctcttgt gaagccatgt caaaaactaa gtgcatggag agatcgtctg
     4081 cataaaaatt ttgaccatat actctttctt tcctaggtgg aagacgtaaa tctatattta
     4141 aatgttttcg tactaattca gcactcttca acatttcatt ataagcaatt tccagaagag
     4201 tttgttcgga taaagcccaa aatggaacta ttcgtccaaa tcggttaata acttcaggct
     4261 ttagaccttc accaatcaag gcttgtttga tccctgatgc gacttttcgg tattctcttt
     4321 ttagttcagt gctatattca ttagaatttt ccttgtttgt aacacgagat agtgagctat
     4381 atctgtttaa cgaatcgaac atctccgcac ctgcatttgt tgtcgcaaat aaaattgtat
     4441 ttgacaatga aacggtgtcg tgaacttctc gatcatccca tcctctttca gtaaaactta
     4501 tcctaccttc atctaaaatt gataaaaagg catcgagtgt ttctacacca gctttctcat
     4561 attcatcaca cacaataatc gcagaaggat catgccttac atgagtacct atgagtcgtc
     4621 taaaacgcat tgcatcatct tttgttttgt agtccggcat attaactaag agaactttat
     4681 tttcatcgcc ataaagtcct cttgccacgg ctttagccat tgcagtttta ccgacccctg
     4741 ttgatcctgt aaacagagca gttgcttttg gttgctttcg acctttaaaa cgatcaaatc
     4801 caagcttcat acgtttaaac aacattgtta tagtataaac tgctaacggt tgccctttta
     4861 tggactttaa ttcctcttga agtttttcca tatcaaaatc agaatctagc tgaatattat
     4921 acttaccaaa tatttgcaat agactttctc gattataagc atttccgtct actcgatctt
     4981 ttgcaatcaa atcctccatt acggcaattg atttagccgg ttcggacaaa tcctcacgat
     5041 aaagcttgtt tgcttgtacg atttcttgaa gtaattcatc aggaatcctt tgcgataatt
     5101 taaaagaagc ctgaggatca tctttaacag aggatgtatt tagccattgt ctcaaaatat
     5161 cgacgacaac atcacttttt agttcattca attctaacgg ttgtaaccgt ctccctaagg
     5221 catcatctct tttgatatag ttttcatatt cttttcttgt agtagcggta atcaattgaa
     5281 gtgattcatt tgctaacggt tcttttagcg aatcaccccc aattttattg ccactaccaa
     5341 aagagctaac aactttatga aactcgtcaa tgaagaggac aaattcattt tctttgtcct
     5401 cagattttaa aattcgctca tattctttca atcttggaat taatgtttct aaacgtgttt
     5461 ggaggacatc atctccccca acacgcaatt gtccgattga taatttgtat acttgtactt
     5521 tctttccatc ttgtcgctgt ctttcagccc attcacttgc aagtgctgtt ttacctgttc
     5581 cggcttcacc taacaagata gcttgcggtg tttccggtct attcaagatc agtgacaact
     5641 catggagttc gtcatcacgt cccactaact tatgtttcgg tggtttcatt ggtgtgagag
     5701 catgattaaa ttcagtttga atattcttaa aaaactcggt actattttcc atctgtttgt
     5761 gttcacttcc tctaaaataa gaataattta cttctagtca gtcagagtta ggtacttaaa
     5821 tgtcgtcttc atgtctccaa aagaatcttt gttaaagtca gcatttttgt attgatcaat
     5881 caatttttgt gtatcattga ttgctttaat ttcggtctcc attcgactgt ctagttgacg
     5941 aatactttct tgcgaatctt tatcgtcagg attttgttgt aaacgttcca gatactcagc
     6001 ttttgtagct tcaattttct tgatgttagt atttgaagca tttaactgat cttctagctt
     6061 atttacaaca ttattcttac ttgtaactgc tacaactttt gaataatcaa tatcgccatc
     6121 tttggtaaga aagcttcctt gatagacatt agttgaatct gaatatggat ttattctgaa
     6181 attgatatta tcaggagtat taacaggttt tgcttttttc ccaaaagaaa ttactccttc
     6241 attattaaca tcattcatag aatattgaga aagtgctttt gtaactgaat ctgcttcatt
     6301 cacattgact ttggtatcat catcagctgt atcatcacca gaaattagtg atacttggtt
     6361 tgcaataaaa acttgataag ctctttcatc taatttacct tttaggaata agaaagcatc
     6421 cccatcagta cttaacattc catagctcat ttcaagcttc ttaggacgat ctttttcttt
     6481 tgttgccata tataagttgt agttttttcc ttgcgtagat aacaactttc tagcgttttg
     6541 cgagtaaccg agttttacta ctgttacatc tctgttttga tctgtccaca catctgaaat
     6601 agtgatctct gctcctgatc gagcaaactc caatgaagag cctataggtg ttgtattgtt
     6661 cacgagataa taattatagt ttcctaattg ataaatgtag ctacccaagc ctgttaataa
     6721 aaaaagcact cctagaaaag ttgcaagcat aatgcgtaat ccgtgacgtg ttctcttatc
     6781 cttatcacca agcaaccctc caataatact gtattgatct ttggtagatg ttgaattgac
     6841 gttttttttc attagtttgt ccgccccctt tcataaaaga gcaaacaatc agacgttttt
     6901 tcggtaattc taggttcggg atttttaatc atttcggtat attcttcccc attaatattt
     6961 aacacaaaaa ttatcttcct ttcttcgttc ctttcttttg ctctttattt tccaaataat
     7021 caatgatttt tgctattgta ccaagatagt aattcccctc ggctttgttt tttcggattt
     7081 tgctaaccat tggagtaggt acaatctctt catctctcaa ttcactgaaa gattttttac
     7141 ctagatattt tccaataaca gattttaaaa aacgttcaaa atctttctcg taagttgttt
     7201 tcttgccttt tagttcatgg ttcatattgt caacgacttt tgcggtttca ataattgttt
     7261 tagttctcag cttaataatt tcatctggaa ttttcaatcc tcgtaatttc atcgttgtac
     7321 taactggtaa cccatgcttt actgcatcag caatactaaa actttttaga tcatctagta
     7381 agctctccat atagttaaca aattcatgcc aattaatagc attcattgtc tcaccccttc
     7441 atttttttaa aatgaggttc gggcgtaaca catttatgtt attataatat cataataaca
     7501 taaataaatc aatcagcaaa tttagatatc ccttaaagta tcagcaatta ttcaacaaga
     7561 tatttatttt agctatttca gtcatcatga aagactaatt agcaataaaa taactaatat
     7621 tatccattta tcagacgaaa agaaaaaact tatctaatta ctctatatta acttttaaca
     7681 aatagttgac ttgtccaaaa atgttacctt caatctcaca aagatattaa gatgtttcct
     7741 accaaaactg atatcaccgg actttttatt ctgtgttgtc caaaagtgtt acctttagtc
     7801 gatcaacaaa ttaaaaaaat tgtttttttc ttgacttgtc caaaagtgtt acccatagtt
     7861 gttaaaattt gtatagatta gccattcttt caaatattat ttagactgaa acttgcttac
     7921 aaaaaaactt cttgcattta ttgtccaaaa gtgttaccca tactttttta aagaagcagg
     7981 ttgtattaaa attacaacct gcttcttctc aatcttcttc taaccaatta tgcattgaaa
     8041 tggttggcaa gtttgattct ggctcactaa aagtgaatct gagccgatca actcttcttc
     8101 ctttctttgc atagattttt tcataatcaa aagtttcaaa tacctgtatt ccgttttcat
     8161 caggagctaa aaattcttct ttcgcctgct ctagtattct tttgtcaata tttgtcattc
     8221 tataacttga aggtatactt aatgctcttc taaaatcttc aactttgact acccaataac
     8281 catttcgcca ctctttattt ctgtattgca tgagatgacg atacaattct tttgtgtagc
     8341 tacttcttag ttttgtaaaa ttatcaagtt caaaacgagt aaaatttttg ccaattgagt
     8401 ttaagataaa ttcaaattcc tcatttgcct taattgtaac tgtttccttc tgtttatcta
     8461 tccggaattt ttgaaaaagc cacagctgtt catagacgtc tgaatcttca aaacggtagg
     8521 ttaaagtacc tagtttgtca ctcaaagctt ttaatacaga ataaaaactg tccttttctc
     8581 ttctgtcgta tttgaccaaa taacgaaaga ggttgaaatc aaaagttact tctgctgttc
     8641 cttgacgttt catttttgat aaaatagccc aaaacacatc catttcaaca ggcttaaatc
     8701 ctcgcaaagc tgttgtattc agaccattgt tataacgcac aattgcattt gtttgttctg
     8761 atactaaatc tacatcacta tcaataatct tttcggtaac ttcttgagac attgattact
     8821 ctccaatctt ctttttgcgc tcctttaatc atgttaatct ggtactaatt aaaggaaaca
     8881 tttttttctg cttgttacct aaattaaatc acaactaaag gtaacagtca acaaagattt
     8941 gttactaatc taattttcta ttttttcgcc ttccaaatca agaaaaccga aggtaacgtc
     9001 ttatcctagt atgatacctc aaatgtccac ttttgttacc tttgctgtcc aaaagtgtta
     9061 cccttactgt ccacttttgt tacctttact gtccaaaagt gttaccttta taccccttta
     9121 atacttactc tcccaagaga ttaagcctcc cctaacagat atattaacag atatttaaca
     9181 gatatattaa cagatattta ataaaggagg attactcctc tcttatttca aaatcttaaa
     9241 aaaaaactaa cactatctag gagtttattt tgcttgtgta actttgaata attgaaaaaa
     9301 aggaaaagga ggaagcttca gcttcccctt ttatttatat ttat
//
LOCUS       DACECY010000089         9281 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.094, whole genome shotgun sequence.
ACCESSION   DACECY010000089 DACECY010000000
VERSION     DACECY010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.094"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            806..1006
                     /locus_tag="IWO38_002488"
                     /pseudo
     CDS             806..1006
                     /locus_tag="IWO38_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1493..1672
                     /locus_tag="IWO38_002489"
     CDS             1493..1672
                     /locus_tag="IWO38_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314521.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(1718..2323)
                     /locus_tag="IWO38_002490"
     CDS             complement(1718..2323)
                     /locus_tag="IWO38_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0314522.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2505..2840
                     /locus_tag="IWO38_002491"
                     /pseudo
     CDS             2505..2840
                     /locus_tag="IWO38_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3005..3232
                     /locus_tag="IWO38_002492"
                     /pseudo
     CDS             3005..3232
                     /locus_tag="IWO38_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3358..4329
                     /locus_tag="IWO38_002493"
     CDS             3358..4329
                     /locus_tag="IWO38_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0314523.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4469..5782
                     /locus_tag="IWO38_002494"
     CDS             4469..5782
                     /locus_tag="IWO38_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0314524.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5825..6691
                     /locus_tag="IWO38_002495"
     CDS             5825..6691
                     /locus_tag="IWO38_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0314525.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6688..7536
                     /locus_tag="IWO38_002496"
     CDS             6688..7536
                     /locus_tag="IWO38_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0314526.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7550..9022
                     /locus_tag="IWO38_002497"
     CDS             7550..9022
                     /locus_tag="IWO38_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAQ0314527.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQKRIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            9039..>9281
                     /locus_tag="IWO38_002498"
     CDS             9039..>9281
                     /locus_tag="IWO38_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0314528.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         2951 a         1562 c         1895 g         2873 t
ORIGIN      
        1 tctatatttt gatatacaaa ttacagcttc agatactaag ttgcataaac cttttccgga
       61 ccccatacaa aaggcaattg atgaattggg cataaaaaat aagaaacgat ttatattggt
      121 gattcattat atgacatgca aagtgcgaaa gcttgtggga tagcatttgg tttagctaaa
      181 tggggagcca aaaatataga aaattttcaa agtgtagata taattattga ttctcctcaa
      241 gaaatacttg atttgttaga aaaatagaca aagagcgtga gataaggata gggtctcacg
      301 ctctttgttt ttcgccattt aaagattgaa atttttgcca tttaaaaaga aaagtgacag
      361 ataggaataa aaacttatta aatgtagtta tgatctgtta tatttcattt ctaatactag
      421 gtcttccaca agcagtgttt cctgaaatgt tttctggaga ctggggtatt gtcagctatt
      481 ctatattaac attgattgct atggttttag gtacttcatt ttataatgct tttgtaagta
      541 agcatgttga ggaatatgaa gtattaatgt acttaaaaga gaaaaaaaca agtaattaat
      601 atgagaacac cacgcatagt ttttattttg agatattcta gaatgaaaca cattaagaac
      661 gaaagcaaag caaaagacgc cagaacttct actaccttct aagttttttc atatttgttt
      721 gttatgctat acatagatct gcataattag acttgcgctg cccgttattg aagtttcagg
      781 cagcgctctt tttgggggat cgataatgga agaagaaaaa atatttgaga aacgctggca
      841 gctagcatca agtgaacaaa gagcaagata caataatttg atcacctcct accccactgt
      901 tgactagaca tataaggaaa agaaatactt actttggctg tgccagttag atatagatac
      961 ctttgaaaca tttgaagtga tcctagataa aattaaacag aattgatcag aaacaagcga
     1021 agccttctct cggttcgctt gttttgctat ttattagttg gctcagggca actctaagct
     1081 cgtttgaaag aaaaccgatg gataatcccc ttgtttgtac cactttagaa tacatggatg
     1141 tacgtattgc cattgactcc tattagttac tgaaaaaaaa tttaaacaag ttgagtattg
     1201 cagataaata gctccagtga ggtgaataac tgctccactc aaggcaatag atagctccat
     1261 ccagggcaac aaactgctcc actttcatat ttaatagatg aataaccttc tgtataatag
     1321 aaaggcatac ctttcggtat gacaacttat acagaatgtt attttattgg ttcactatcg
     1381 aaaaattttg gagctttatt tttccggtat ttctcaacga acgattagtt taactgtggg
     1441 aagctccaga catacgattc gagaagttac ttctcgagca gacaggaagg gggtgactga
     1501 actaaccgct gatatgagcg atacttggct gcaggaatat ctttttccgg aaaaacaaac
     1561 aacagcacga ggttaccttc ctgtagactg ggaatatgtt cataaagaac taatgaaaaa
     1621 gaatatgaca ttgaaactcc ttcatagaga atatggggta catggaacat agaaacgaaa
     1681 atgcacggta gaaaagcgaa tgaatatagt ataaattcta caaatcattc gctttcttta
     1741 ttttatatat ggttggtcga gagagaccat tttctttggc aatttctgca actggaactc
     1801 cttgatttaa catgtccacc acagttttaa agactaatcg tttttgcgga tctgttgagt
     1861 tctcagaata ttcaattggt cgccccttat atcgaccagc ttttttggcc aataatactc
     1921 cttgccgctg acgttcacgg attttctcac gttcgttttg tgcaatataa cttagcaatg
     1981 ataaaaacat atcgaacata gcagtatcta ggagttcatt tccagtatta aaatctaaaa
     2041 actgtgcatc tagtattttt aatgctactt ttttctgttt cataaaggta actgtatttt
     2101 ttatgtcttc ataatctctg cctaggcgat ccagtgatgc aacaatgaca caatcacctt
     2161 ctcggatata atttagtaat ttttgaaact caggtcgctg gatatcttta ccactggctt
     2221 tttcttggaa taatttatca atgttaaggt cttccatcat tgccaattgt cgatctaaat
     2281 tttgatcttg tgaagacact ctaatatatc caatttttgc cataaaaact ccttttaaaa
     2341 aatgatataa tttacatgta aaatttactt gtagttttac tgttatcata gcatttttaa
     2401 aaataaattt taagtctact tataatttac tttggaggtt ttgaatgaat gaaattaagc
     2461 gcaataaaaa ttcttttggt gtttatttag gagagcctag ctcggaacag ttaaataaag
     2521 gtgaagctcg tcatgctcta tgtcgaaata tattttatgg tagaaaaggc aaactttatc
     2581 aaacttatta cgacggtatg gaggaacagc tcaacgcatt aggattagta actaatgcta
     2641 tcatatattg gaattcacta tatttagaaa aagtctttga acaaattaaa acggaagggt
     2701 tcgactgttc cgaagaagtg ttacgcaagc aatttccatt aattagtgat cacattaatt
     2761 ttgttggcaa atatagtttc cgctatgaaa aagaattatc gaatggaata ctacgtccgt
     2821 taaatataga gaattattga gaatgttcgt cttttggttc ctaccgtgca ttttcgcacg
     2881 gatgttccag atactatttt tgaatgtata aaattgaaaa cacaagaata ctaataaatc
     2941 aacgattctt gtgtttttaa tttttcacta ccgtgcattt tcgtttctat gttccatgta
     3001 ccccaatctt gaaaaattaa ttaaagactt agataaagcc attgacgact tagtcgtggt
     3061 gctaccggag tatcaatgtt taaccagtat cccaggtatt ggtcctgttt aggctgctgg
     3121 aatactcgct gaaattggtc caattgaacg tttttctgat gaaataaaat tgacaaagta
     3181 tgccggccta tattggcgac agagtcaatc cggtaaatct gaatacgaaa atttttttac
     3241 gaaaagcttc acgtctaata tctattttaa aataaaaata ttcctgctaa tttggaatcg
     3301 tttccttctt ttggtataat aatacctgaa attcttttga gaggagttgt tcgtgaagtg
     3361 aaaactacta tgaaggatgt tgctgcgtta gcaggagttg gtgtgggtac ggtatctaga
     3421 gtaataaatg gagtaaaggt gaaagaggtt accaaagaaa aagtaatggc tgcaattagt
     3481 gagcttaatt atgagcctga tgaatatgcc agaggtatga aaacgaatag tagcaataca
     3541 atagctctta ttttacctac gatttggcat cccttctttt ccgagtttgc ctactatgta
     3601 gaggaagctc tcagccaaaa aaattataag ctttttttgt gtaatgcgaa cggcagtgcg
     3661 gaaaaagaaa aagaatatat ccaaatggtc aagcagagta aagtagacgg aattataggt
     3721 attacctatt cagacattga taaatatgta tcatcaaatt taccatttgt cagtatagac
     3781 cgccattttt cggaacaggt ttcttatgta acggcagata attatttagg tgggcaaatt
     3841 gccgctcaag aactacttac tcgtaactgt aaaaacttgt gctatgttgg gggtgtttca
     3901 ccttacccta acgaaacaaa caatcgtaaa gctggttttt acaatatttg tgaggaaaat
     3961 aaaatagctg tgaaggtatt ggatatgcct gaaccaatta gcaatttaga ggaacaattg
     4021 ctcgcttttt tttcggataa tcctgaaatt gatagcattt ttaccatcaa tgatttcatg
     4081 gctttggatg tgataaaagc ccaaaaaaaa ctagggaagg aagcacctaa ggattttcaa
     4141 attattggtt ttgatggagt caagctatcg ttgcaaaaag actatttact atcaacgatt
     4201 gtacagccga tagaggaaat ggccaaggtt tcagtagaaa cactcatggc gatgatttta
     4261 aatgaagaac cgcctaagcg tgtggtagta ccggttttct ttcacgctgg agagacgact
     4321 aaagagtaaa aaaccggttt ataacgattt ttttgagata ggttgacaga aagtgatttc
     4381 aatgatatct taaaaccaat taattggaaa cgttaccaat taattgaaaa ataaaacgga
     4441 aacgtttcaa aaaataggag gaacatcgat gaaaaagaga atgttagcaa ttgcagcaat
     4501 tttagcatta acagctggag gacttgcagg atgtggcagt aaggagaaaa gttcaggtag
     4561 cagtgatcca ggagaaatta agatttgggt tcagttttct gatgaaacgc ctgaaggaaa
     4621 agcttgggca gagattgtga acaattttaa tgatgaatac gagggtgaat ataaagctgt
     4681 tactgagtat attcctcgta gcggaagcgg cggtggatat gaggataaag taaatgctgc
     4741 aattacaacc aatacccttc ctgatgttat tacgttagat ggaccgaata ctgcagctta
     4801 tgctcattca ggagtcattg ctccattaga tgattatttg aaagatgcgg acatggatga
     4861 tgtattagac agcattattc agcaaggaac ctacgatggc aaaatgtatg cgtttggttt
     4921 ctcagagtcc agtgtgggtg tctattacaa taagtcgctg ttcaaagatg ctggaattac
     4981 agaagatcaa ttgccgacgt tagaagatcc ttggacttgg acggaatttc aagcaatttg
     5041 ccaaaagctg actgataaat acaaaacgcc tgcaattgat atgcaaatgg cgtcgggaga
     5101 cgaaatgctg acctacgcat atacaccatt tatatggtcg aatggcggcg atgtcgttaa
     5161 tgctgatgga accaaagccg aagactattt taacggtaaa aaatccgttg aagcgttgga
     5221 atttattcaa gaattggttg aaaaaggtta caccacaatc agtcctattg aaaaaggatt
     5281 tgaaactggc aaatatgcca tgatgctgag cggttcttgg acaatggcag atttagagga
     5341 aaactatcaa gagattgatt atggtatttt gccttacccc gtttcagatg caactgggga
     5401 gttagtttca ccaaccggaa gctggcagtt agcaatgact gagaaaacag agaaaaagga
     5461 tgcttctgcg gcctttatta agtttgcaac cagtacagaa tctagtcgta ttatgagttt
     5521 agggaacagt gttctaccta tcagaaattc aacagttgat ttaattaaag atgaagtgtc
     5581 ggaaggaatg caggtattaa ttaagcaaaa ccaactttct ggacatgccc gcccagtggt
     5641 tgtagcttat ccgcaagtat ctagagcatt ccaacaagca gttcaagata ttagctactt
     5701 cgaggaaaac ccagatgtac aaaaagtagc agatagtcgc gctgctgaaa tgcaacaggc
     5761 aattgatact tcactaaaat agaagtttct aaagggggcc aatcaagtgt taaaaaaacc
     5821 gacaatgaaa gacaatttga taggttacgg ctttctagcg ccagctttgc tgctgctgac
     5881 gatttttctg atcatacccg taatcatggt aatttattat gcctttactg attattattt
     5941 gttaacgcca gatgctagac aatttatcgg cttagaaaat tttaagcgac tgttcgctga
     6001 tccaattttt gtaaaaagta tttggaacac ggctaaattt gttgtttgga ttattccggt
     6061 ccaaataggg gcagctttag gaatggcact tattatcaac aaaaatcgta aaggaaatat
     6121 cttttttaaa gtagcctttt ttgcgccagt cgtgatgtcc ttagtcgtta tttcgatttt
     6181 atggttgtac ttgttaaatc ctaatgaagg tctggttaat acgctatttg caaagattgg
     6241 aattgcttcg cagccttttt tgactagtcc caaacaagca atgtatacgc ttgttttcgt
     6301 atcggcatgg caaggtgcag gatatcagat gctgctgttt ttagggggaa tgcaaaatat
     6361 tccgcaagac gtttatgagg cagccgaaat tgatggtttt tctaagtttc agcaatttcg
     6421 ctatatcacc atgccattat taaagcccac ggcgattttt attttactta ccacattgat
     6481 ttcggccttt aaactaatca ttcagccaat ggtaatgact caaggtggac caatgaattc
     6541 tactatgaca atggtttatt acatttacca aactggattt acagaccgta tggtaggcta
     6601 ctccagctcc attgcgttaa tctttaccac tatgattggt ttaattacaa ttgctcaaag
     6661 aaaattggta aaggaggatg atccagcatg accaagaaat ttggaccgct aatgatcgta
     6721 gaatattgtt tgctgttcct tttagcggca ttattcattt tcccgatgct gtggatggta
     6781 gtttcctcta tgaagcctga agcagatgtc tatcaaaatt tagcttcctt caaagctttt
     6841 ttacctagtt tgaatccagc taattggttt aaaacttatc aagaggttat ttcccggttc
     6901 agtgtgggaa cttatttatt aaacagtatt ttttatggtt taacctttgc ctgtgggtca
     6961 atcattgtaa actctttggc gggttttgcc tttgcgaagg ttaatttttc aggcaagaaa
     7021 attatctttg gagctttgtt agctctgctg attgtcccga tggagacggt tttaattccg
     7081 caatttacca tcgttaattc actaggctta gtgaatactc gcttggcagt gattttacca
     7141 gcgatggcca gcgcgtttaa tatctatctg tttagaaatt tctttatcgc gattccagaa
     7201 gaaatcattg aatcagcgaa attagatggt gcaaatattg ttaaaatttt ctttcacatc
     7261 atgctgccaa tgtctaaacc agccatagcc acagtaggtg tcttatcctt tatctccagc
     7321 tggaatgatt atatttggcc gttaatggtc ttaactgaca gcagcaaatt ttcaatgcaa
     7381 gtggcaatta ctacgatcaa tactacacag ccagtttata tcaatcaagt gatggctgtt
     7441 ttaaccattg caacgattcc actgattatt gtttatatcg ttgcacaaaa atatattctg
     7501 cagggacttg gcggttctgg aacgggtata aaataggagt gaatagtgaa tgttgcaaaa
     7561 acttttaaag gccaatgagt ttattgagaa aaatgctcaa acagtcaatc aagaataccg
     7621 gaataaattt catttgatgg ctccgattgg ctggatgaat gatccaaatg gttttgttta
     7681 ttatcaagag gagtatcact tgttttatca atattatcct tacgacagca tctggggacc
     7741 aatgcattgg ggccatgcta aaagcaagga tctaattcac tgggagcatt tgccagtagc
     7801 actggcacca ggtgaaagct atgacaagga aggctgtttt tccggcagtg ccatagaaaa
     7861 ggatggcaag ctgtatttga tttatacagg acacaatgtt gtggaagggc aagttcgaca
     7921 agttcagtgt ttggcagttt cagaagatgg cattcacttt gaaaaatatg ccggaaatcc
     7981 aatcatcgca gaagatcact tagcttctgt tgcaacgacc gaagatttcc gcgatcccaa
     8041 agtgtttcag cgaaaagaag actattttac tgtagtcgct actaaaactg cagatgctcg
     8101 tggacaaatt gtgatgttcc attccaaaga tttactaact tgggagtttt attccgtatt
     8161 gctggaggga actccagaac aaggcattgt atgggaatgc ccagacttgt ttgaactaga
     8221 tggcaaagaa gtcttgattc tttctcctat tcaaatgact ccacaaggca atgcttacca
     8281 aaatacgaac tcaactgttg ctttcatcgg tactgtcgat tggcaaacag gcaaatttct
     8341 agtagagaat tttcatgaaa tcgatggcgg tttagatttt tatgcacccc agacctgtga
     8401 aaacgaagca ggccaaagaa taatgattgc ttggatgcaa atgtggcagc gtaaaatacc
     8461 tacgaatgat ctgggccatg gttggtcagg ttccatgacc ttgcctagag agctgagggt
     8521 ggctgataat caattgttgc aacggccagt ttcatcaatt tatcaagtaa ttgatccgca
     8581 gaaaaggatt gaatgggcga agactgaaga gaatcgcttc ttactgaagg acttagtata
     8641 tgaccagcac tatttacgct tggaaatcaa tttaaaacag gcaacgaaac tagaacttca
     8701 ctatgcgaag ggacccgacc atacattact gcttaactat gatgttgaac gggagtgctt
     8761 tactgcttcc agggaacaca tgccgtatga tttatcagga gctgaaaagg aagtgttgaa
     8821 aaagcgcagt gtgacgattc ctttggaaaa taattgtcta accatcgaaa ttttccgtga
     8881 tacttcagct attgagcttt ttctaaatga tcgtctgact atgagtacaa ctttttatga
     8941 gacagaaaaa ggacaggatt ttcttttgct ggcagacagt gaagtaacct tttcattgga
     9001 aagcggctta attaatctat agaagggaga gctcagtgat gatttatggt tcaatcgaag
     9061 caggcggcac aaaatttgtg tgtgccattg gcgatgaaga aatgacgatc aaggaaagag
     9121 tgagctttcc gacaacgaca cctgaggaaa caataccttt agtcattgac ttcttcaaac
     9181 aataccaagc agatttagca gggattggga ttggctcatt tgggcctatc gatatccatc
     9241 gcgattccgc cacttatggg tacattacct cgaccccgaa a
//
LOCUS       DACECY010000090         9278 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.095, whole genome shotgun sequence.
ACCESSION   DACECY010000090 DACECY010000000
VERSION     DACECY010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.095"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..435
                     /locus_tag="IWO38_002499"
     CDS             <1..435
                     /locus_tag="IWO38_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ0314529.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="IWO38_002500"
     tRNA            complement(759..842)
                     /locus_tag="IWO38_002500"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="IWO38_002501"
     CDS             1370..1870
                     /locus_tag="IWO38_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0314530.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="IWO38_002502"
     CDS             complement(2060..2404)
                     /locus_tag="IWO38_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ0314531.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="IWO38_002503"
     CDS             complement(2558..3478)
                     /locus_tag="IWO38_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0314532.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="IWO38_002504"
     CDS             3665..4156
                     /locus_tag="IWO38_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ0314533.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="IWO38_002505"
     CDS             complement(4180..6555)
                     /locus_tag="IWO38_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ0314534.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="IWO38_002506"
     CDS             6881..7507
                     /locus_tag="IWO38_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0314535.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="IWO38_002507"
     CDS             7530..8234
                     /locus_tag="IWO38_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0314536.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="IWO38_002508"
     CDS             8426..9259
                     /locus_tag="IWO38_002508"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ0314537.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2828 a         1790 c         1651 g         3009 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttct
//
LOCUS       DACECY010000091         8776 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.096, whole genome shotgun sequence.
ACCESSION   DACECY010000091 DACECY010000000
VERSION     DACECY010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.096"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1..633)
                     /locus_tag="IWO38_002509"
                     /pseudo
     CDS             complement(1..633)
                     /locus_tag="IWO38_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            654..1854
                     /locus_tag="IWO38_002510"
                     /pseudo
     CDS             654..1854
                     /locus_tag="IWO38_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288612.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
     gene            2588..3556
                     /locus_tag="IWO38_002511"
     CDS             2588..3556
                     /locus_tag="IWO38_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0314538.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            3567..4382
                     /locus_tag="IWO38_002512"
     CDS             3567..4382
                     /locus_tag="IWO38_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAQ0314539.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKMGNEFAGKSCLVDGSFGFGIHYD"
     gene            4450..5160
                     /locus_tag="IWO38_002513"
     CDS             4450..5160
                     /locus_tag="IWO38_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0314540.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKQMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            5196..6695
                     /locus_tag="IWO38_002514"
     CDS             5196..6695
                     /locus_tag="IWO38_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAQ0314541.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKNVSTEDFFNYCRLEESG
                     VTVTSGTDLPLFITDIPESIIRAVFRRFPEGEEVWEKETSFTTTELLKSFTSNSFKVN
                     GLSEYGEIKVGKSASLAVFSEDLCTDDWQKVMAAEVIATYLDGDLVYKK"
     gene            6743..8143
                     /gene="yfcC"
                     /locus_tag="IWO38_002515"
     CDS             6743..8143
                     /gene="yfcC"
                     /locus_tag="IWO38_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ0314542.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFVGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            8176..>8776
                     /locus_tag="IWO38_002516"
     CDS             8176..>8776
                     /locus_tag="IWO38_002516"
                     /inference="COORDINATES: protein motif:HMM:NF013429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0314543.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAFTYSNCQAVKQLAQKHALDIIAFTPESIIYPYNIAAPETMQWEKSKAYFRNAILA
                     TAELGCSLMTINSGYGLMEEPIEESWKRSREMLRYLAEIAETEGVTIALETLRPEESK
                     IVTKLQEAQRMVHDVAHPSLKLMVDTAAMGVAGEGLQDWF"
BASE COUNT         2829 a         1389 c         1835 g         2723 t
ORIGIN      
        1 aaaggcattt gaaatacccg attggtagcg atggaagatc gtgaatttct ttttaaacga
       61 ctgaggtaac gtagatgaaa ttgttttaag ggtttcttga aagcctaccg tatctcgttt
      121 aacgaaataa tagcgtaata attgacaaaa atgatacgca gttcgcaatt cttcgttgta
      181 gcttaacagc tcattaacga tttctgtttg actaattggt cgtttaaaca gtcgatgata
      241 atgccgtttg agtgcctccc atgtcgtgga atctttgagt aagagtttcc aatatcgctt
      301 gagccgacgg tacttcttct gattttcaga gtgtgaggtt cgaaaacgat tcatgatctg
      361 gacacgtaat tgattgaagc tgcgattgat gtgttggaca atatgaaagc ggtcagttac
      421 gatctccgca tttggaaaga ccgttttaag caactgacaa tagctggcat tcatgtccat
      481 cactaggtac ttgaccttta aacgggcctt tctaggaaag cgcaggaagt attgagttag
      541 tttgtaaagt ctgcgatctt caagggtcgt aaatagctga ttggttgtcc catcaacaca
      601 aataaaactc atggaaccag aagtggcaac attgtgtact tttctttgca atcttgtgta
      661 caaaactatt gcaaaacaca gcgcataaga atccttacaa aacagtagat gaagcaaccc
      721 aattagatct tgttatagtt gttaatcagc tcttaacggg ttacgattca aaatgggtca
      781 acaccctata cttagataag tcacttgaat atcagaacat aattcaggct ttttcacgca
      841 caaatcgtct taatggtcct gaaaaaccgt tcggaaccat tcgttattat cgtaaagcac
      901 atacgatgga acagaacatt gaagaggcta ttgagttata ctcaggcaaa caagcaaaag
      961 ggttatttgt tgaaaaatta tataataact tagaaaagtt gaacgatata ttcgaagaaa
     1021 ttaagtccgt atttaatatt tctgacatag aaaacttcag tcatttaccg gaggttccag
     1081 gggaaattaa gcaatttgta aatctttata atcaactaat ccattgttat gaatcagcga
     1141 tgatacaggg attcacatgg gcaaaatcga cttatgaaat ccaggattat gaacctataa
     1201 cagtagtaat cacagaatat gaatttaata tcttggcaca acgttacagc gagatagaga
     1261 gaacctagag acggaaatga tagtatagcg tatgacattg attaccaatt acctgaaggt
     1321 agtcgtagtc gtattaatta tgagtattta aacacaaact tcaaaaaata catgaatata
     1381 aaagaagaac gtgattcgaa tttaattcaa tcattgttaa acgacttaca tcgcacattt
     1441 gcgcaattac cagtggagtt acagccatat gctgagatga ttctaaatga tgtaaataat
     1501 ggggaattag ttatcaaaga aggatgggaa tttactgatt acctcaatga atatcagctt
     1561 agtgaagagg acgattttat ccaagattta gtggattcaa ccaatattaa tgaggcatta
     1621 ttacgtgaga tgttggattt acgcttaacc gaggtaaaca ttaatgaata tagtcgattt
     1681 gataaattaa aatcaagtgt taacgtaggt catttcagtg gatatatcga gaaaaattta
     1741 ggtaaaatgg taataccaat caaggtaaat atgctaattg acagattatt gcgtgcattt
     1801 ttattagaag atgtattcga tgttacagag tacataaaga attactttaa ctagagtaca
     1861 cctacatctt atagtacata aaaaaattca tcttttttag tttaataatt caccaaaaag
     1921 gtgagtaatg ccatttttga aaattgagct tgttttatgt ctacatctgt atccgtatat
     1981 aatccaaagg attatcaact cagaagagtc tttatttttt ctgaatataa ctactgtatc
     2041 aaaaacaatc gattatgaga caaaacaaaa tgacctcttt tatttaagaa aggaggtcat
     2101 tttttgtatc ataaattgta ttaaaatcaa tgtatcactt tttaatcagt tttaagacca
     2161 taaattgcgt actaaaagaa attctgcaat tcaaaataaa gcattttaag ccattttcaa
     2221 atttaagtgc acccatctac ccgtcaaatc actttatttt ccactccagt agtttcctaa
     2281 ttcaactgtg tgcaaaaacg atttcttcta aacaaatcaa gacaaactta taaaccttca
     2341 tcccagcacc ataattattt aaatcatagg catttttcga aattagtata tgtactcaag
     2401 tttcactgca gattatttat attttttaga gaaataaatt tttcctattg acaaacacaa
     2461 agaaagcgga tacaatgcaa ttatgtcaca tgacgttata tgacgttatg taatatcttt
     2521 acatttaatc agttactttt attttggata aaattaacat ttttaaaatt ttaaggagga
     2581 aatgttgatg gatgtagcaa aaacggttaa cgcaattgta gaaaaaaatg atggagagat
     2641 ttctggaatt tatttggtgg cttgtggcgg ttcgctggta gatatgtatg tctcagatta
     2701 ttttcttaaa gccgaagcat cgaagtgtat tacaggctta tatacagcga acgaatttaa
     2761 ccatgtaacc ccaaagcaat tagatgataa atctgttgtc attctatgtt ctcatggagg
     2821 aaatacagca gaaacggtaa aagcaggaga agtcgctcaa gctagaggag ctgtgactgt
     2881 tggtctgact cataataaaa ctgcggattt attaaaggtt tctgatcata gtttccttta
     2941 cgaatgggga gatgatgcaa aaatagtcaa taatcctatg gccatcattc ttgatttaac
     3001 tgtatctttg gtcaatgtta ccgaaacatt tgaacattac ggtaaattca gagaaggcat
     3061 gaaggttatc gataccgtaa ttgaaaatgg cattaagcag gtgcaggatc gtattaaagt
     3121 gtttgctgat aagtacaaag atgaaacaat gttttatatc ttaggtagtg gaccatcata
     3181 tggacatgct tatggttttt caatttgctc tttgatggaa atgcaatggc ttgattcaac
     3241 ttctgttcat tctggagaat tcttccatgg accttttgaa gtaacagaca aagatacgaa
     3301 ttttattctc ttgatgagca ttggacgcac tcgtgcttta gatgaacggg caaaacgatt
     3361 ccttgataaa tatgcagaaa gagtagaaat cgttgatgca aaggaactag gattggatat
     3421 gatatcagat gaagtttctg aattcttcaa tcccatttta ttctacagta ttctttcaga
     3481 atatcgttca gctttagcag atgtacgcaa tcatgattta gatgttcgtc gctatatggg
     3541 gaaagtggag tactaggagg attaaaatga aggtattagg cttaggtgat aacgtcgtcg
     3601 ataaatatga aaatctggga attatgtatc ctggaggaaa ttcattgaat tttgcagttt
     3661 ttgcaaaaaa actggggcat gaaagtagct ttatgggagt ttttggctca gatgaggagg
     3721 cagctcatgt tttatcagca ataaaagaaa taggactgga caacagtcac tctcgctttg
     3781 aaaaaggcga gaatggctgt gccagagtga agattgatga gggcgaccgg attttccttg
     3841 gcagcaattc gggaggtgtt actcgtgaat acccgattca gcttactgaa gaagacagag
     3901 aatatttaaa ccagtttgaa ttgattcata tgggattata tagccatgtc aatcatctat
     3961 tagaagagtt acaaaatgtt tctggaaaga tttcttatga tttttctgac gactttacgg
     4021 aagaagaggt tcagcgggcg attgataaag ttgattttgg cttcttttct atagaagact
     4081 tttccgatga agaagtaaag cagtttttga tgaagcattt ttgtgagcga aatgatgtat
     4141 tgattgcaac ccgaggagaa aaaacggcta ttggttatga tggagcaaag ttttatgaag
     4201 tgaaccccgt gctagaagaa ccagttgata caatggcagc gggagattct ttcttaacag
     4261 ctttcctact ttattttctc gaaggggacg atatagtcac cgcgatgaaa atggggaatg
     4321 aatttgctgg gaagtcttgt ctggttgacg gttcgttcgg ctttggtatt cattacgatt
     4381 gagcttgttt taaattcctg catttgataa cctaaagaaa gaaatcccgg taaagtggag
     4441 gttaaaaaaa tgctggatac aaatgcacaa ttaccattgt acttacaatt aaaagaaata
     4501 ttaaaaaata aaatcaatac aggggagtat cccgagaata cgaaaattcc cgctgaatca
     4561 gagctgatag aaagttatga ggtcagccga atcacagtga gaaaagcagt agaagaactg
     4621 gtagaagaag ggtacctact taaaaaacaa gggaaaggaa cttttgtcaa ccggcataaa
     4681 gtttttcgta aaattgaata tgttatgggt ttcagtgaga gttgtaaatc taatggtttt
     4741 acaccgacta gcattgtttt ggaacgcaag atcattccag caactgaagg gatggcaaaa
     4801 aaactcgata tcgctcaagg tgatgatgtt ttatatatcc agagaaagca aatggcggat
     4861 aacatcccca ttatgttaga aaacaattat ttttcttatg aaagatatag tttcttaatg
     4921 gaggaagagc tgactgattc tttgtatgaa gtacttaaag tccaaggtac cttagccatc
     4981 aatcctgggg aaactgcact tgaaattgtc acagctgatg accaaattgc caaaataatg
     5041 gaagtagctg ttgggacgcc ctttttcttt atggatactt taatcaacga tcaaaacaac
     5101 ataccaattc atgtaggtaa tcaatattac ctgggagaag tatatcgttt ttacttatga
     5161 tttcctgttt tgatttcaag gagtagattt tatttatgaa gcaaatattg cggtcaaaaa
     5221 tgatttttga tggagaacag ccacctttca aaggttatat tgtaatcaat gaagggatca
     5281 ttgaaaaggt ggaaaacaac tggaattaca gtgatctatt aaatgaaaac acggaattaa
     5341 ttgcttatga tgatcaattc gttatgccag caattcatga taatcatgta tttttcagtg
     5401 gttatttggc tatgaatgct ggcatagatt taagcgatac atcttcagct aacgaggctg
     5461 tagagcgtat taaagctgta atgaaccata aaaaagcaga tgaaccaatt tatgctcatg
     5521 gctgggatgc tgccctgtgg aaaactgacc catcagaaga gctattgaat gaaataaacc
     5581 actttggtcc aatcacagcg atcgacaaaa atcgcagcca ttgttggatg aatgctattg
     5641 ctaagcagac ttatggtttt acagaaaaag aaaccagtgc tgaaagtcga gtattattaa
     5701 ttaaggaaat gctagcaaac aaaaagcttt tagcagaggt ttacgctgaa tttgaagcat
     5761 tactgctttc aaaaggtgtt atatcaatga aagaaatcgt ttttgatgac gcagaatttc
     5821 tttctaatat acctcagagg aaaatactta gtaacctata tgtgcaggca gtccaagaac
     5881 ctctacagca tgaacttcta tatgagtata aggaaaaagt tttccctgaa aaggtccgct
     5941 ttggtggagt gaagatcatg gtagatggtg ttgttgcgga tagaagtgga gatatttacg
     6001 gtgtttatca gtcaggagta gcttcacccg agattgacta tgaggcaatt aaagcagaag
     6061 ttgctgcatt caatgaagta gagattcctt gctgtttgac aacggaagga gataaagcgg
     6121 gttataaagc tgcgtcaatt cttgtcgaat ttggaaagcg gttacctgaa ggcgttttta
     6181 attcgattag tgatttggaa atggttactc aaaagacagc agaaaaaatg aacgaaggac
     6241 aagtagtagc agaggtatat ccacaaattc tcggcttgaa tcaaacgtta gaagaggctt
     6301 acatgtcaac tgttataaaa aatgtttcca cagaggactt ctttaattat tgtcgattag
     6361 aggaaagtgg tgtaacagtc actagcggta cagatttacc gctgtttatt actgatattc
     6421 ctgaatcgat tataagagca gtattccgtc gttttcctga aggagaagaa gtctgggaga
     6481 aagaaacaag cttcacgaca accgagctgc tgaaaagttt tacaagcaac agcttcaaag
     6541 taaatggttt atcggagtac ggagaaataa aagtagggaa aagcgccagc ttagctgttt
     6601 tcagcgagga cctctgtact gatgactggc aaaaagtgat ggctgcggaa gttatcgcta
     6661 cctatcttga tggtgactta gtttacaaga aataatgctt ttcgatataa cgttataaga
     6721 cgaattaaag agaggttaaa aaatggaaac aatgaaaaga aaattcacct tcccacatag
     6781 ttatacattg atttttatct taattatttt ggctgcaatt ttaacttgga taattcctag
     6841 tggtcagttt gagacagttg ttgaaaacgt cgcaggtatg gaaaaaacaa cggttgttcc
     6901 gggaacctat caccaaatcg ataaggcaga ttatggacaa ggtttttcct ctatactaat
     6961 ggcaccagct gaagggatta tttcagctgt tgaggttgtt gcttttgttc tcatcgttgg
     7021 tggtgccttc gggattatcc taaaaacggg agccatcgat cgagggctgt ttaccttagc
     7081 aaaagttctg ggagacaagg ggatcttagt tattccgatg tcgatgattc tattcagctt
     7141 aggaggatca acctttggca tgagcgagga agtcattccc ttatttgcgg tgtttatttc
     7201 tctgatgttt tctcttggct atgattccat gactgcgatc ttgattttat ttttaagtac
     7261 acaagcggga tatgttgggt cgacgattaa tcccttcaac gttttaatcg cacagggagt
     7321 agctggcgtt tacggaaatc ccctgctttg gtttcggatg ctatcctgga ttgtcattac
     7381 tgggatttca attgttttca ccatgcttta tgctagcaga gtcaaaaaga atccactgtc
     7441 ctctattgtt tatgagagtg atttaaaggt taagcataag tttcaaaatt ttgacactga
     7501 aaaagagttt tcaacacaag ataaactaat tatcgccggt tttatcgtag gtttaggaat
     7561 catgatttgg ggtatcattt ccaagggatg gtatatggtt gaaatttctg cactattcgt
     7621 aggattaggt ctttttgctg ggggcatcgc tagattttct caaaaggaaa ttgctgaagc
     7681 ttttgtcgaa ggctgttcag atttcgccta tgctgctgta atcattgggc tagctagagg
     7741 agtcttggta atcttggaag gtggaatgat tattgacact attctaaaca gcttggctaa
     7801 ccttttaggt ggtcttccaa aattcctgtt cactacgatt ctactcattt ctcaagtaat
     7861 tgtttccttt tttgttccat cctcctcagg agctgcggca ttaacgatgc cggttatggc
     7921 accattgggt gatctaatga atgtaagtcg tgatgttgtt gttctaagta accagtttgg
     7981 aaatggttta atgaatatta tctccccaac tagtggtgta ttactggcag gattagccat
     8041 cgcagagatt aatttctcta aatggtttaa ggttggcttc aaaatatttg ccatcttaac
     8101 ccttgtttct gctgttttgt tatacattgc tacactcttt tagtcttaat catccattaa
     8161 taggaggcaa tagttatgat taaaataaat caattagcag gaatgaatat ccactattta
     8221 ttttattctc tcaaagaatt tttcgcttat caacagaaaa ttggtatttc ttctgttgaa
     8281 ctttggggag gtgctccgca ttttttcata gatgctttta cttattctaa ttgccaagcc
     8341 gttaagcaac tagcacagaa acatgctctt gatatcattg cttttactcc agaatcaatt
     8401 atctaccctt ataacattgc tgcgccagag acgatgcagt gggaaaaaag caaggcctat
     8461 tttcgaaatg caatattagc tacggctgag ttaggctgct cactgatgac aatcaactcc
     8521 ggatatggtt tgatggaaga gcctatcgaa gaaagctgga agcgctcaag agagatgcta
     8581 cgatacctcg ctgaaatagc agaaactgag ggagtcacga ttgcattgga aacgttgcgt
     8641 cccgaagagt cgaaaattgt aaccaaatta caggaggctc agcgaatggt tcatgatgtg
     8701 gcacacccat ctttgaagct gatggttgat accgcagcaa tgggggtcgc tggagaaggt
     8761 cttcaagatt ggttta
//
LOCUS       DACECY010000092         8431 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.097, whole genome shotgun sequence.
ACCESSION   DACECY010000092 DACECY010000000
VERSION     DACECY010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.097"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..785
                     /locus_tag="IWO38_002517"
     CDS             <1..785
                     /locus_tag="IWO38_002517"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314544.1"
                     /translation="KDKIFKFLLLFTMPILLKTVADGYLSRNNIAVYLAFIGIYLVKE
                     KYIPKTVAKFSLLIIIPIGLFLFYIMSQIRLGTQSDISFWQGTWNLIDSESSYPQYYD
                     YANQGNSNVNIINFIIYIFIVCIPSSFYDLIHFSIPNLAYTFTETILGLSYGETNNYY
                     ILLPSVLGEAIMLCGKYFAFTYAFIYGIVSTWFLRLLKRNKSLEYLLIYYLLDFFRQF
                     RGGSQYVITAWETQLVPLIIIVFFYKLVIKNGVAKERNEYNV"
     gene            769..1734
                     /locus_tag="IWO38_002518"
     CDS             769..1734
                     /locus_tag="IWO38_002518"
                     /inference="COORDINATES: protein motif:HMM:NF021616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0314545.1"
                     /translation="MNTMCEVSIIIPSYKGSNEILIAVWGALSQTVTSKEIIVVDDNG
                     IGTEEQIKTKEKLQKFIKENKIKYFAHEKNLNGSAARNTGIKASNGKYIAFLDDDDYI
                     IPSKLEEQINVLEKTPKEVGMSVCEGYYVHKDGKGYLKKIKGNYVALDKYLLDKHYFN
                     TSSIVIKRECLIDMKGFDDSFIRHQDWEFCTRMLSRYKSIVVHKPLFIKYSFNRNNPS
                     NIEKRIENLDFFFEKMEDKLKDNLSLNIVNRIYKFKYGQIYQQYIINREFKAAKKFKN
                     QYHINIFTSLKDLVIHVIKRYSIALKKRTLSYEEMLVELERGMNY"
     gene            1734..2600
                     /locus_tag="IWO38_002519"
     CDS             1734..2600
                     /locus_tag="IWO38_002519"
                     /inference="COORDINATES: protein motif:HMM:NF025663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ0314546.1"
                     /translation="MNIHNIGKKILCRTFPIIMTKYFYRKILGRKLKLKKPETFNEKI
                     NWYKLYFCPQNDLVIQCTDKFRVREYVEDMYCGDSLNDLIGVWDGVDEINWNSLPQRF
                     VLKCNHGCGMNIICPDKTSLDIEETKNRLNKWMKMDWGKESIEPHYSKIKPKIICEKF
                     IENTLDKESSLPPDIKIHCFNGIPKVIAYYSGRGNDMHGTFYDVNWNKLDFATKEGSV
                     ISKPICLDKMLLYAEKLSNPFPYVRVDFYECNNIPVFGELTFTPASGRSPYMTREADE
                     YMGNLLNLKEME"
     gene            2602..4107
                     /locus_tag="IWO38_002520"
     CDS             2602..4107
                     /locus_tag="IWO38_002520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HAQ0314547.1"
                     /translation="MRTKKAFYNTTTGIIYQLCSIVCGFIIPRLILGYFGSEYNGMIS
                     SITQFLGWISILTAGVGGVTRAALYKPLNKNDNYKISGIINATDNFMKKIALIFLGGL
                     IIFAIGYPFLLKNKFEWFFVFTMVLVLGINTFAQYFFGMTYQNLLEADQRQYIYSTVR
                     ILTTLISTLVTVVLIIMHFDIRIVKLASSLIFAVNPIFLNIYVKKYYSIDTKIAPDNS
                     AISQRWHAFAQEIAVMINNNTDIVVLTIFSNVLEISVYVIYNMVLSGVKQLLQTFTTG
                     IGAAFGSMIAEGIDNSSLNRKLKVFEVLVYNITTILFSVTLVTIVPFVRVYTSNINDV
                     NYIRPVFAIIITIAAMFNCFRIPYQTIVYAAGHFKQTRNGSIVEVILNLSISIILVIK
                     IGLIGVAIGTLVGSIFRTLQYGIYLSRNIIVRSNWLFYTKIVISLLETMIIYIISSIF
                     INISNVSYFGWISYAINIFMLSIAIVCMGSFLFYRKDTISIIKRLIFALKR"
     gene            complement(4405..4965)
                     /locus_tag="IWO38_002521"
                     /pseudo
     CDS             complement(4405..4965)
                     /locus_tag="IWO38_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177795.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5199..6491
                     /locus_tag="IWO38_002522"
     CDS             5199..6491
                     /locus_tag="IWO38_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0314548.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCLISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYIRRLWRPGFKAHLTETDIVDRLLKRSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            complement(6779..8263)
                     /locus_tag="IWO38_002523"
     CDS             complement(6779..8263)
                     /locus_tag="IWO38_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAQ0314549.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRVESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDIPQNMMSAIVDSNRTRKDFIADRVLQKTGYYSYDDDN
                     TFDLSLEKEVIIGVYRLTMKSNSDNFRQSSIQGIMKRIKAKGATVIIYEPTLKDGSTF
                     FGSKVINDLEKFKSISHSIIANRYDSILNDVKEKVYTRDLFQRD"
BASE COUNT         2857 a         1182 c         1429 g         2963 t
ORIGIN      
        1 taaaagataa aatatttaaa tttcttctac tatttactat gccgattctt ttaaaaacag
       61 tggctgatgg atatttatca agaaataata ttgcggtata cttagcgttt attggtatat
      121 acttagttaa ggaaaaatat attccaaaaa cagtggcaaa attttcttta ttaattatta
      181 taccaatagg actattttta ttctatataa tgtcacaaat aagacttgga actcaaagtg
      241 atataagctt ttggcaagga acatggaatt taatagatag tgaaagttca tatcctcaat
      301 actacgatta tgcaaaccaa ggaaatagta atgtaaatat aattaatttt attatatata
      361 tatttattgt atgcatacca tcaagcttct atgatttgat tcatttttca attccaaatt
      421 tagcgtatac tttcacagaa actatattgg gtttgtctta tggggagaca aataattatt
      481 atattttatt accatctgta ttaggagaag ctataatgct ctgtggtaag tacttcgcat
      541 ttacctacgc gtttatctat ggtattgttt caacttggtt tttgagattg cttaaaagaa
      601 ataagtcttt agaatattta ctaatttatt atttattaga tttctttagg caatttcgtg
      661 gtgggagcca gtatgttata actgcatggg aaacccaatt ggttccttta ataataatag
      721 tattctttta taaattagtg ataaaaaatg gagtagcgaa agagaggaat gaatacaatg
      781 tgtgaagttt ctataataat tccaagctac aagggaagca acgaaatact aattgctgta
      841 tggggagcgt tgtcacaaac agtgacatcg aaggagataa ttgtcgtaga tgataatgga
      901 attggaacag aagaacagat aaaaacaaaa gaaaaattac aaaaatttat aaaagaaaat
      961 aaaataaagt attttgcgca tgaaaaaaat ttaaatggtt cagctgcaag gaatactggg
     1021 ataaaagcta gtaatggaaa gtatatagct tttttagatg acgatgatta tattattcca
     1081 tctaaattag aagagcaaat taatgtatta gaaaaaacac caaaagaagt tggtatgtca
     1141 gtatgtgaag gatattatgt tcataaagat ggcaaaggtt atttaaaaaa aataaaggga
     1201 aattatgtcg cattagataa atacttatta gataaacatt attttaatac gagttctatt
     1261 gttataaaaa gagagtgttt gattgacatg aaaggctttg atgattcttt tatacggcat
     1321 caagattggg aattttgtac gcgaatgcta tcaagatata aatctattgt ggttcataaa
     1381 cctttgttta taaaatatag ctttaatcga aataatccat ctaatataga gaaaagaata
     1441 gaaaacttag atttcttttt cgaaaaaatg gaagataaat taaaagataa tttatctcta
     1501 aatatagtta atcgtatata taaatttaag tatggacaga tatatcaaca atatattatt
     1561 aatagagagt ttaaagcagc aaaaaagttt aaaaatcaat atcatattaa tatattcaca
     1621 tcattaaaag acttagttat ccatgtaata aaaagatact ctattgcgtt gaaaaaaaga
     1681 acattgtctt atgaagaaat gttggtagaa ttagaaagag gaatgaatta ctaatgaata
     1741 ttcataacat aggaaaaaaa atactttgca gaacttttcc tattatcatg acaaagtatt
     1801 tttatagaaa aattcttgga agaaaactga agttaaaaaa acctgaaaca tttaatgaaa
     1861 aaataaattg gtacaagtta tacttttgtc cacaaaatga tttagtaatc caatgcactg
     1921 ataaatttag agtaagagag tatgtagaag atatgtattg cggtgattct ctaaatgatt
     1981 taattggagt atgggatgga gttgatgaaa ttaattggaa ctctctacca caacgttttg
     2041 tgttaaaatg taatcacgga tgcggaatga acatcatttg tcctgataaa actagtttag
     2101 atatagaaga aacaaagaat agattaaata agtggatgaa aatggattgg ggaaaggagt
     2161 ctatagaacc tcattattca aaaattaagc caaaaattat ttgtgagaaa tttattgaaa
     2221 atactcttga taaagaatct tctttaccgc cagatataaa aatacattgt tttaatggga
     2281 tacctaaagt tattgcgtac tactcaggta gaggtaatga tatgcatgga acattttatg
     2341 atgttaattg gaataaatta gattttgcta ctaaagaagg ttcagtcata agtaagccta
     2401 tttgtttaga taaaatgtta ttgtatgcag aaaaattatc taatcctttc ccctacgtta
     2461 gagtagattt ttatgaatgt aataatattc ccgtttttgg agagttaact tttacacctg
     2521 caagtggaag atctccgtat atgacgagag aggcagatga gtatatgggg aatttgctaa
     2581 atttaaagga aatggaataa aatgagaacg aagaaagctt tttataatac gacaactgga
     2641 atcatatatc agttatgttc tattgtatgt ggttttatta ttccaagact cattttagga
     2701 tattttggat ccgaatataa tggtatgatt tcatctataa cccaattttt aggatggatt
     2761 agtattttaa ctgcaggagt tggaggagtc actagagctg ctctttacaa accattgaat
     2821 aaaaatgata attataaaat aagcggaata attaacgcaa ctgataattt tatgaaaaaa
     2881 attgctctca tatttttggg gggacttata atttttgcta ttggttatcc atttctctta
     2941 aaaaacaaat ttgaatggtt ttttgttttc acaatggtat tggttttggg aattaatact
     3001 tttgctcaat atttttttgg aatgacatat caaaatttat tagaagctga tcaacgccaa
     3061 tatatttatt ctactgttag aatattaaca acactgatca gtactttagt tacggttgta
     3121 ttaattataa tgcattttga tatacgaatt gtaaaattag ctagttcatt aatttttgct
     3181 gttaatccta tttttttgaa tatttatgtt aagaaatatt acagcataga tacaaaaata
     3241 gcaccggata attcagcgat ttcgcaaagg tggcacgctt ttgcacaaga aattgcagtt
     3301 atgataaata acaatacgga tattgtcgtt cttactatat tctcaaatgt attagaaatt
     3361 tctgtatacg taatttataa catggtttta agtggagtta agcagttatt acagacattt
     3421 actacaggta taggggcagc atttggaagc atgattgcgg aaggaataga taatagtagc
     3481 ttaaatcgta agcttaaagt ttttgaagta ctcgtttata atataactac tattttattt
     3541 tctgtaacat tagttacaat tgtaccattt gttagagttt atacttcaaa cataaatgat
     3601 gtaaattata tacgtccggt attcgcaatt attattacaa ttgctgctat gtttaattgt
     3661 tttcgaattc cttatcaaac gattgtatat gcggcaggac atttcaaaca aacaagaaat
     3721 ggttctatag ttgaagttat tctaaatctt tcgatttcta taattctagt tattaaaata
     3781 ggactaatag gcgttgcaat aggtacatta gtgggttcta tatttagaac gctacaatat
     3841 ggtatatatt tatcgagaaa tataatagtg cgctctaatt ggttatttta tacaaaaatt
     3901 gttatttctc ttctagagac aatgattatt tatataataa gctcaatatt tataaatata
     3961 agtaatgttt cgtattttgg ttggattagt tatgcgataa atatatttat gttatctatt
     4021 gctattgtat gcatgggtag tttcctattt tataggaaag atactatttc aatcataaag
     4081 agattaatat ttgctttgaa aagatgatat aaaaagtatt tttaatttga gaatatctat
     4141 tgcgactatt agactattaa tttggttgag gattagatag tcgtgaaatg tttattctat
     4201 tacattagat tttttagtaa taactatata aattatctaa attaagattt aaatgatagc
     4261 ttatagaaaa taaccttatt ttagataata tttctattaa ttatgtttaa ttgattgcat
     4321 tttgtagaga gtgggacaat agtcctttct ccaaaagaaa acaagtccga ttttgatggt
     4381 taatttcaaa atcggacttg ttttttcttc ttttaaataa ggcaagattt cttctttata
     4441 aagggcataa tagaattcgt ttgattgctt atccaaagca gtttcataac gattcgtttc
     4501 tttacgccag aagaaactga acttgttggc atctgcttca atttttgtac catcaataaa
     4561 tattgcttca ttatcaatta cttaattggt gatcgattgg cggctaaata agacaaacgc
     4621 ttcagctaaa aggtcagcgg gtgtttcctg acttcggaat cgattgatgg tccgatagct
     4681 gacttgttcg tggttagcca accaacacat acgatagctg tcatctaata gaaattcaat
     4741 tttccgacca gaaaagacac gctgcatata agcatataga ataattttta acatcatttt
     4801 ggggtggtag gccggtcgtc ctagttattc ttataattca agaaagagtg tttgtggcat
     4861 tgctacgact aaagcgttga ctgcaaaagc gacatctgtt tcaggaatat attgttccat
     4921 atctaatggt aaagttgttt gattgatggg ataattttta aacataaagt actcgcttcc
     4981 tttctgattg ggcttggtca ctttaatctt acagggatga gtgctttttg tgtaccttaa
     5041 aatcaaaaaa agtggggcaa taatcagatt ttggctctat actttttgat gttggtggat
     5101 gacaccagca tcttttttat tttccaaaac atatagaaaa gacaccttga aatcctttag
     5161 aattaatttg gcgaaccaac ccaaaaggag gaatcaagat gtcttacact caccttatca
     5221 aagaaacatt agatattcta gacttatcag ttacttttaa cgaaaattgt ctcacaaaag
     5281 aaaaatacaa agggcaaatc tgtcatattt atcgtggaaa cttgatttat acagcccaag
     5341 aatgcatcca ctgtaaacac cagattgctt cagatattgt gcgttggggt acaacaaccg
     5401 tccggttgtt aatgaacgat gtttctgaat accgtaccta cctcgaattg aaaaagcaac
     5461 gcttcaaatg caaagcttgt cagcggacct ttgtcgcaga tacttctgtt gcgaaaaaac
     5521 attgtctcat cagtgagaaa gtccgctggt ctgtcgtgac tcgtctgaaa aaaaatacct
     5581 caatgacaga gatcgccgct cagaaaaatc tatcggtctc ttctgtttac cgtattatga
     5641 agcgatttta tcgtccgttg aatccttttc ggacaccgtt acctaaggta ctttgttttg
     5701 atgaattcaa atcagttcgg ggtgtttcag gtgctatgag tttcatcatg atggatggac
     5761 aaacacagcg cttattggac attgtcgaga accgacaact gcctttttta aaacgttatt
     5821 ttagtcattt ttcacgagaa attcgtgaag cggtcgaatg gatcgtgatt gatatgtacg
     5881 ctccttatgt atcgctcgtt aaaaagctgt ttccaaaagc acagctgatt attgaccggt
     5941 ttcacatcgt ccaacatatt ggtcgtacct ttcgcaatca tcgaatcaaa gaaacgaacc
     6001 aactattaaa aagcaaagaa caaaaacact atcaattagg taagcaatta aaaagatact
     6061 ggaaacttct tcaaaaagat gaacgcaagc ttgattatat tcgacgcctt tggcgacctg
     6121 gcttcaaagc tcatttaaca gagaccgata ttgtggatcg cttactgaaa aggagccctg
     6181 ctttgcgtgt aggctatcag ttatatcaag actttcttta cgctgtaaag gaacgagact
     6241 acgtatcgtt tgaagaacta cttaccaata atatcatgct tcccgaaggc tatcagacga
     6301 ccttgagaac gttccagaaa tttctgccac aaatcaaaaa tgctttacaa caaagttatt
     6361 caaatggccc acttgaatgt ctgaacaacc atatcaaagt gttaaaacga aatgcatatg
     6421 gctttcgtag cttttataat ttcaaattgc gcatcatgat tcgtcatggc aacgcattga
     6481 ttttcaacta aaaaagatca agaggaactg atccacttga tctccaatta ttctaagatt
     6541 tctctttacc aacatcattt gacaaagagc ccagattttt ttctgactat tgtcccactc
     6601 cccttgcgtt gtaaaaattt tgataattag atttcttaaa gttggtaatc ttttattaaa
     6661 aatcttttct aaagagagac gtaattcctc ctagcgctta attaatgggt ctaaatgaaa
     6721 ggataaaaat tagtcttgct gtttattgta aattatttta atgtatctaa tcattcgttt
     6781 aatcccgttg aaataaatct ctagtgtata ctttttcttt tacatcatta agaatactat
     6841 catatcgatt agcaataata ctatgactta tgcttttaaa tttttctaaa tcatttatta
     6901 cttttgaacc aaaaaatgta ctaccatctt taagtgttgg ctcgtaaatt atcacagtgg
     6961 ctccctttgc cttgattctt ttcataatac cttgaataga agattgacgg aagttatccg
     7021 aattactttt cattgtaagc ctatagactc caataatcac ttctttttct agagacaaat
     7081 cgaaagtatt gtcatcatcg taagaataat atccagtttt ttgaagcacg cggtctgcta
     7141 taaagtcttt tcttgttcgg ttactatcga caattgcact catcatattt tgaggtatat
     7201 cgttatagtt agttagaagt tgttttgtat ctttcggtaa acaatagcct ccatatccaa
     7261 aagatggatt attataatgt gtgccaattc ttggatctaa gcaaaccccg tgaatgattt
     7321 cttgtgtatt tagtcctttc atttctgcat aagtatccag ctcattgaag taagatactc
     7381 gaagtgcaag gtaagtatta gcgaataatt taactgcttc agcttctgtg aatcccatga
     7441 aaagagtttc gatatctttt ttgattgctc cttcttggag taaatctgca aaagtgtgag
     7501 aggcttctcg taaagttttg tcagtttggt ccgtgccgac aataattcgc gaaggatata
     7561 gattgtcata taatgcctta gattctctga gaaattctgg gctgaataga atatttttag
     7621 tattgtattt tttacgaata ctttcggtat agcctacggg taccgtcgac ttgatgacca
     7681 ttattgcctt tggattgact tctaacacca atttgattac tgactctaca gcagaagtat
     7741 caaaataatt ttgtttacta tcataatttg ttggagcagc aatgacaaca aaatcggctt
     7801 ctctataggc ggctgcacca tcagtcgttg caattaaatt aagatttttt tcagctagat
     7861 atttttcaat gtaatcgtct tgtattggag atttcttatt gttaatttgt tttacttttt
     7921 caggaagaat gtctactgct gttacctgat tgtgctgggc aagaagagta gcaatggaaa
     7981 gacctacgta accagtaccg gctacagcaa tattcttcga ctgatgtttt gttctgtttg
     8041 atttatttga ttcatttata aatagatctg ttggttcaaa atttagaact tctgctaatg
     8101 attctaattg ttgtattgac gggataaaat cgagagattc tactcgacta attaaggcac
     8161 ggttgatacc agtagctact gcaagttgtt gttgtgtcat tttcagagat tttctagaat
     8221 caataatgat ttgagctaaa cgtttttgag ataacttttt catgatgcac tccttttttt
     8281 gttgctaata ataacaaaca attttttctt ctataacaga atagcttatt attaataaaa
     8341 ctgtcaattt ttttattgat taaaaagata ttttttgatt ttaagtattt tcaattattt
     8401 tttgttactt aaaataactt tatagattgt g
//
LOCUS       DACECY010000093         8290 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.098, whole genome shotgun sequence.
ACCESSION   DACECY010000093 DACECY010000000
VERSION     DACECY010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.098"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(45..1511)
                     /locus_tag="IWO38_002524"
     CDS             complement(45..1511)
                     /locus_tag="IWO38_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ0314550.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1898..2494
                     /locus_tag="IWO38_002525"
     CDS             1898..2494
                     /locus_tag="IWO38_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0314551.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2552..3034)
                     /locus_tag="IWO38_002526"
     CDS             complement(2552..3034)
                     /locus_tag="IWO38_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314552.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3140..3337)
                     /locus_tag="IWO38_002527"
     CDS             complement(3140..3337)
                     /locus_tag="IWO38_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314553.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3632..4390
                     /locus_tag="IWO38_002528"
     CDS             3632..4390
                     /locus_tag="IWO38_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ0314554.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4450..4878
                     /locus_tag="IWO38_002529"
     CDS             4450..4878
                     /locus_tag="IWO38_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314555.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4924..4996
                     /locus_tag="IWO38_002530"
     tRNA            4924..4996
                     /locus_tag="IWO38_002530"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4957..4959,aa:Gln,seq:ctg)
     gene            complement(5146..5517)
                     /locus_tag="IWO38_002531"
     CDS             complement(5146..5517)
                     /locus_tag="IWO38_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0314556.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5732..5917
                     /locus_tag="IWO38_002532"
     CDS             5732..5917
                     /locus_tag="IWO38_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314557.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6000..6188)
                     /locus_tag="IWO38_002533"
     CDS             complement(6000..6188)
                     /locus_tag="IWO38_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314558.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6212..6475)
                     /locus_tag="IWO38_002534"
     CDS             complement(6212..6475)
                     /locus_tag="IWO38_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314559.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6633..6890
                     /locus_tag="IWO38_002535"
     CDS             6633..6890
                     /locus_tag="IWO38_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ0314560.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6961..7152)
                     /locus_tag="IWO38_002536"
     CDS             complement(6961..7152)
                     /locus_tag="IWO38_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314561.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7293..7613)
                     /locus_tag="IWO38_002537"
     CDS             complement(7293..7613)
                     /locus_tag="IWO38_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314562.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7636..8070)
                     /locus_tag="IWO38_002538"
     CDS             complement(7636..8070)
                     /locus_tag="IWO38_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0314563.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
BASE COUNT         2628 a         1635 c         1314 g         2713 t
ORIGIN      
        1 aggaactgct gcaaataagc aattcctgtt tttatgttca ttgattaatg aatagagccc
       61 attagttttt tgattggttc tttcacagta aatagcaaga taccaccgac gattgctact
      121 gcaccgataa taccgaagta agcagattct gttcccggtg tgtagaagcg tgcaatttgt
      181 gcattgattg cttgtgaaga agcatcagct aagaaccaaa tagcgattgt ttgtgattca
      241 aatgctcgtg gtgctaattt tgttgtcaca gacaaaccaa ctggtgaaag acacaattca
      301 gcaataacca cgatgaagaa acttcccaac aaccacaatg gacttacttt tccacttgta
      361 ccataaagca tacccggcaa catcaatagc aagtacgata atcctgcaaa taaaagtcca
      421 agagagaatt tcactactgt agatggttga cgttcaccaa gtttcgtcca catcgttacg
      481 aatactggtg tcagaataaa gatgaaaatt ggattcaatg attgatacca gcttgcttgg
      541 atcgttacac caaatagcgt agattgtgta cgttcactag caaataaagc taagatagat
      601 gaaccttgtt cttgtaatga ccagaaaatg attgctgcaa ggaataatgg gatataagcc
      661 catactcttg gtttttcttc agcagttaca tcttttgaag tcagcatttt gataaagtaa
      721 tacaacggta agataatccc caatacactg attgtattga caaaaaagtc aattgtcaga
      781 tgaccagtta gttgtgcccc gccgaatact gcagcgataa caacaagtgc gatgatcaag
      841 ttacggatga atcctttacg ttcttcagtt gtcaaaggat tcggcggtgt tgtacccact
      901 ccgtctaatg tcttgcgtcc ttggatgatg tattgtaata atccaaagaa catcccaata
      961 gcagctaaag agaaccctaa atgatagtta tattcttgac cgattgtacc aacgattagt
     1021 ggtgctaata atgcccctaa gttgacaccc atgtaaaaaa tcgaaaatcc tgcatctctt
     1081 cgcaagtcag tctttgaata aagatgtccg accatgccag aaacatttgg tttcaaaaag
     1141 gcggttccga taataatcaa tgccatcgaa acaaacaatg tacttacacc aaatggtaaa
     1201 gccaagacga tgtggccaag cataatgaaa actccaccga agaatacagt gcgttttgct
     1261 cctaacactc ggtcagatac ccatccgccg acgatactag acatgtagat caaagatccg
     1321 tagatcgaca tgatagctaa tgctgttgct cgtggtaatc caagtccccc gttggcaact
     1381 gtatcgtaca tataatagat aagtaatgca cgcataccgt aataactaaa tcgttcccac
     1441 atctcagtga agaacaaagt tgacaatccc ttcggctgcc cgaagaagcc cttgtcttgc
     1501 tgtttttcca taatctatac cccaattctt taaattttca gaaaatatta tacatcttta
     1561 ccgaaaaatt taactaaaaa acttatttaa gaaatataaa ctaattttct ataaaagcaa
     1621 gtaattttta atggcaatat cataaaaaaa agaagataga gatttaataa ttatatatca
     1681 gctgtttttt tcaaataagc aaaaattcct aaaacacaac aatgaaagcg ataactaaga
     1741 aaatccttac ataacgattt ttcttattaa aattgttttt tgaacttcgc ttattttttg
     1801 tgagcatatc tgtttactta ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa
     1861 aaaaatgcaa aacgcaaatc aaaggagaaa ttttattatg acaactttta ctgacacttt
     1921 gaaaaaccgc cgttcaatct atcatctagg acgtaatgtt tcactttcta atgaagaatt
     1981 aacaactttg atcaaagaag caattaaaga aagcccaact gctttcaatg ctcaatcaac
     2041 acgcgcagtt atccttttcg gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc
     2101 acttcgccca ttgactccag cagaagcttt cccaaataca caaaacaaat tagctggttt
     2161 caaaaatggt tatggtacag tattattctt caaagatact gacgtggtaa aaggtctaca
     2221 agaacaattt gaattatatg cagacaactt cccagattgg tcagaacaat caaacggaat
     2281 cgcaacagct aatacttggg tagcattagt tgataaagga ttgggcgcaa acttgcaaca
     2341 ttacaaccca gttattgacg aagcagttgc aaaagaatgg aatatccctt caaactggaa
     2401 actacgttcg caattagtat tcggttcacc agaaacaccg gctggggaaa aagaatacat
     2461 gaacgatgcc gatcgcttcc gcgtatttgg ataattttat ttgaaataaa aaacagaggt
     2521 tgtgacaaac atcttgtcgc aacctctgtt tttattctgg atcaatcata atatcaatct
     2581 ctgcctcatc gtttaagcgt tgggacaaaa atggaactaa ttcttctaat gatgcagaaa
     2641 atttcagcat cggtcctcgc catccgtttc ggaaaaaatt catttttacg attttagaac
     2701 cttttggctg atataataca attttattga tctcagaaaa aggaacacct tttttgtcaa
     2761 agctattcaa aacaagtcct tcttctgtga ttcctctact gtccaacaaa aaactaataa
     2821 aaatcattgc tgacaagatc ccttttgcca attcatctgc tgtaggatga ctgatgagtg
     2881 tataccaaat gactagtgac actaagaagg gtatcaccaa gtagacccat cgatttcgca
     2941 cagtccgata aataatttct tttcgaacca gccaaagaaa taaaaacatc acaacaacga
     3001 ataaaaacaa tagaaccgta aagaatgtta ccatgacaac tcgcctcgct ttttcttctc
     3061 cagaaaatag gaaaagaata ctgatttacc ttctcaaaga aattggactt gcagcacgcc
     3121 gcacgtccaa ttgttttctt tattcaaatg tatcatattt tgcaacaacc atgcttgctt
     3181 tttcatctaa taattcttgg tcctctgcag cacattgggc aactttgacc acggcgcagt
     3241 ttgtcatttt tgatacaact tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg
     3301 catattgttc tcctaaatta atcatttctt ccgtcatcaa aatcctccta aactgctgga
     3361 acttttctcc acattaccac tgctatttct ttggagattt aactcattga tttttattta
     3421 cagttaaaaa tcaaccttac aggcattata gcgtactgtt ttcgttttca caattaaaac
     3481 agctgttgta tttagaagaa tttttttcaa aaaattaaaa catattcctt aaaaactaat
     3541 tatttcctac atattaaaat ataatacttc catttccaat aaaaatacat ttataatagg
     3601 aaagataaac agctgaaaag ggagatttca aatgaagaaa aaaacgttca aaaaactatt
     3661 gtttctaggg ggcacagctg ctcttttatt aagcggtaca agttattacc tacatatcca
     3721 ttctccagaa catacaatga acactgcaca aaaaagttca acacaaaaaa cgaaaaagac
     3781 aaaagagaaa gcagaagttt tactagatgt tccattagag agccagtttg attctccttc
     3841 tttagaaaat ggctgtgaag tcacttcgct ttctatgctt ttatcttttt atggttatga
     3901 aacaactaaa aaccagttag ctgagcaatt ggattacgtc cctgttttca atgcagatgg
     3961 gactcatggc gatcctaatg aaggatttgt tggtgatatc agtggcggag actgggctat
     4021 ggtcgtctat gttcccccag ttgcctccct tgctcagcaa atcgtccaaa cggattatca
     4081 cacttttcca aaaaccgatg ctcaactcga tgatatcaaa aaagcactat ctaaaggaaa
     4141 tcctgtctgg acttctgtga ctatcaattt tgaagtccca gatgaaactg attttatgac
     4201 ttggaccaca aataatggag aagtaagagt cactcctctt gttcatgctt gtgtagtcac
     4261 tggatatgac aaagaaaata tttatgtcaa cgatccctat ggtgtaaaaa atcgagcagt
     4321 accgatcaac gatttttcgg cgattttcac tgcaatgggt ggacagatgc taactttgga
     4381 gaaaagctag taattcaaac aaaaatccct tatactcaag ataccaaacc tttgaaggga
     4441 gcgtagataa tggaaaaatt cacacataaa aaaatggatc caaatgaaat tccgattatt
     4501 tttgtccgtg actgcaaggg aaatgttcaa ggtaaagtct ctattaatga gtggaacgag
     4561 cgtcgcagac ctgctaccct caatgaactg gaaatcaaat tgtatcgtca atctcttgtt
     4621 tattacgcag atcaagagta cgagaaagcc accgatttat tgaaatttct aattgctcga
     4681 acagagtata ctcgctttga atatattgaa cgtttagcaa acatctatca tattatgaat
     4741 gaacctgtca aagaatatca gttattggac accgtcttgt cagtagcaga actgattgcc
     4801 cttcctgctg gattagaaaa gaaactagta cgccgccttt tacgagtaaa gcaacaacta
     4861 tccgatcaag aaaaataact tgcgtttttt taggaaagtg ctatgattat tttgttgaat
     4921 aaatgggatg tagccaaact ggtaaggcac ttgactctga atcaagcaat tgtaggttcg
     4981 aaccctgcca tcccaatttg atgtgcttgc tgaaaaaatg aaccccctgt aaaagaacaa
     5041 tctttttgca gggggattat tgtattattt ttactacttg tatttagttt acataattaa
     5101 attatgaata ataaaatcgg gcgtattctt aatacaaaaa ctgatttacc caataattgc
     5161 taatgctttc tccggaactt tttctctagg aacttcctca tacgtttcta tgtgtgctcc
     5221 tttattgcta attttcaaat aatgattttc ttttaacaca gaaagaccat taaattcaac
     5281 cgttcgttct ttcccattgg catcagcagc tttctgcaca taccttgctg taccatattc
     5341 atttacttcc tctggttttt gtgtctttac atagacctct cctttttcaa ctaatggatt
     5401 tagctgatca agtataccag ggatttctcc ataacttcct tcagtataat atttcagtcc
     5461 aaatagcaaa ataccactaa tcgccgccag tccaatcaaa tatttaatca ctttcatctt
     5521 tctacctcct tgttatatat tcagtatatg ggtcagttaa agaaattcca tccgtcccaa
     5581 gttggagatt tttctcctac tttagaagcg agaatcgagt gtttttttag taaaaatgat
     5641 cgttatttga tattattgac gtgtggataa atcagttttt ctcgttttgt ccatgtaaaa
     5701 aatcatgttg atagcaaagg atgtgttcaa aatgaatcaa gaagaattat ttcaaaaagt
     5761 caaggaaatg attaagaacg gcaatttcga tggcgcaaaa cgatttatcg aagaacataa
     5821 agaacaatta ggaccttata aagagaaagc ccaaaatctt ttaaaagatg taaatatcga
     5881 cagtgtcaaa aacaaattta aaaatctatt caaataagta aaactttcac ttttgtaaaa
     5941 tcaaaggagg acaatgctgc tgcatcgtcc tcctttggtt cttttctgaa tacttcatac
     6001 taaaataaac gtgtcggatc gtaatgataa gtcacattgc cgttcagtga ggcttttccc
     6061 accacatatt tacgattctc caacttcagc cacgcttctc gaaaagccat taattcttct
     6121 ctttttacat caatcttttc gatttttcca tcgatcaaat cttgaataag ttgttcatat
     6181 ggattcattt ttttcctcca tctgctgtca atcaataagt tgtaaaaata ggttcatatg
     6241 cttttttggt atctagatct tctctgactt tgactgtcgt ataaataatt ttttctactg
     6301 ggatactata ataacttcct tcaccattat tgaatcggtg aacgtctttc tttgctaaaa
     6361 taccatttat tttattcgtc agtacacgtt gttcgacact gttacttaca acatagtaaa
     6421 tcacccgaat attattgatc aaatgcaaat cgatttctaa cactacatga tccatgaaaa
     6481 tcccactcct tctttcactt tgattatagc aagtttctcc tctaatttca aaatcgatac
     6541 agattttacc ggataaatcc acaaaactgc ttgcgccatt tcgcacaaag tgttattttg
     6601 tatcatggac tttatttatc ggaggcaata gtatgatcgc gatcggtcaa tttgtttttt
     6661 atattccttt ttttattatg ataagcattc ttttctatta cataaaatgg acaaaaaaga
     6721 agttttcagt tttactggcc tctttacctg ctgtatattt tacttatcaa attttttctt
     6781 ttaggcattg ggaaacaacg tctgtattaa taacacacat catcgaattg acgctttcag
     6841 taatttttct tattatttgg atctattttt tatataagaa tcagaattga atccctcgca
     6901 tgtttaaaaa gaaaacgatt aaagatgcag caaatttctg ccgatcttta atcgtttttt
     6961 ctattctgtt ataccaaaaa aatgtttgaa tcggtgaagg atcgttcctt gttttaattc
     7021 tataaattct tgtgcgatcg ggtacttttc ttccaaattt tctttgttct tttcgatcag
     7081 gcttttcgct tcttcatatt ttttatctgc acatagtgct tttacttcct gtaatacttt
     7141 tcctgcttcc atttgcctca ccctcttgcc attttattgc tttaagtttt atttaagtta
     7201 tacgcattat tctagtttca tcccaaataa ccttttttca tttttactcc tcgaactaat
     7261 cagttttcta aaggctgatt ggttcgtctt ttttattttt cctccatttc attctgctct
     7321 tctgttaatt tatctaatgc tttgattgat gctgcggctc ttgcataatc atagctgtat
     7381 aattccataa atttatcagg cagaacaaac cacgctggat gataaccgtt agctgtgaca
     7441 gggaatcctt tatcttgttg gttatctcca tcccatctca gagcaacacc tttttttcct
     7501 ttccacttta ctaaagcgat cgagtatccc ttctttgttt gtgtgccgtc ttcaataatt
     7561 gctaaaattt ctattttttc ttttggcgca cggacttctt gaggacgaac cattttctat
     7621 tcctccctta tctcattact tcacgaccaa cacactgcaa ggggcatgtg atacgacata
     7681 agaagcggtc gatccaacta atgcttgttg tatagcacct tttcctgtcg ctcccataac
     7741 gatcaaatca atattttctt gtccagggat gacattggca atcaaatttc ttggatctcc
     7801 agtttcaact attgcgtgga tctcagcaat gccgtactct ttggcatcat tgattttttt
     7861 cagcatggct acttctgttc tttgtttttc atctgcatag atttttgaaa aggcataggc
     7921 actatgggtc agctcaactt tgttgatgat tgacacgata aataagtggg cttcattgtc
     7981 ctttgctaag tcaagggctt cagcgaacgc tttgatcgat tgtcttgatc cgtcaactgc
     8041 taccatgatg ttctggtatc ttttactcat tggaaccgcc tcctttttct tttattgtaa
     8101 catctcaagc aaaaatacaa aaaatatacg caatatgtaa ataaaatgat gaacaaaagc
     8161 aatttctttc aagttattta tacatttctt ttctcatcag aacacctgtt ctaatcccat
     8221 tatttctgca ttatgtgaaa ttcctcatca tttttcacat cgagcgaaca tcattctgtt
     8281 acaaaaaacg
//
LOCUS       DACECY010000094         8228 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.099, whole genome shotgun sequence.
ACCESSION   DACECY010000094 DACECY010000000
VERSION     DACECY010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.099"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="IWO38_002539"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="IWO38_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ0314564.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="IWO38_002540"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="IWO38_002540"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ0314565.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="IWO38_002541"
     CDS             2591..3376
                     /locus_tag="IWO38_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0314566.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="IWO38_002542"
     CDS             3389..4240
                     /locus_tag="IWO38_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ0314567.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="IWO38_002543"
     CDS             complement(4364..4816)
                     /locus_tag="IWO38_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0314568.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="IWO38_002544"
     CDS             complement(5281..5649)
                     /locus_tag="IWO38_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ0314569.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5799..6281
                     /locus_tag="IWO38_002545"
     CDS             5799..6281
                     /locus_tag="IWO38_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ0314570.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6318..7328
                     /locus_tag="IWO38_002546"
     CDS             6318..7328
                     /locus_tag="IWO38_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ0314571.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7443..8156)
                     /locus_tag="IWO38_002547"
     CDS             complement(7443..8156)
                     /locus_tag="IWO38_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314572.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2784 a         1337 c         1667 g         2440 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata
     5761 agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat
     5821 tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag
     5881 atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct
     5941 taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt
     6001 tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg
     6061 aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc
     6121 cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac
     6181 cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga
     6241 tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata
     6301 cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg
     6361 ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa
     6421 agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata
     6481 aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg
     6541 aaaatatcca aatgatcttt ccgccttggg gaggagaatt attgattgag attctcgatt
     6601 ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt
     6661 tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaacc aaccttattg
     6721 atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa
     6781 gagaaggaga atcgattcta cagcaacctt ctgaaaagta tcaaaaagag tggccccatg
     6841 agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg
     6901 gagatgcagt aaggatgaag ggacgattat taggtggctg tattgatacg atcagtcatt
     6961 taatcggtac cccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc
     7021 cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt tgatttaaga agatcacttg
     7081 ttcagatgaa actggcagga tggtttgatg gcagctcggg gatcatgttc ggtagaagtg
     7141 cagcagagga tttgttagat tataaaatga aggatgtata tgaagatctg tcaaaggaat
     7201 taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga
     7261 tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt
     7321 ttatttaaaa aattttttcc tgccttggag tttatcaaag accaacaaat ctaaatagaa
     7381 aagaaaatga aagaagctga cagtattttg ccagcttctt tttgtatgat ctattagaaa
     7441 ttttaattat ccataactat cacattgatc agctgtttca catcatttgg attacctgta
     7501 ggaatagcta ctacagatgc attatcaata atttcttgag ttgtttctct aatgaacgct
     7561 ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt
     7621 gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc
     7681 ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct
     7741 gcaggataat gaataacatg tagttcgaaa tcacccgatg tatctctaaa agtattacct
     7801 ctaattgcta gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga
     7861 tctattgact gagaagactg tgaggaagtt gtgtctattg ttgaagaaga aatatttgtc
     7921 tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa
     7981 gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt
     8041 tgtttgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac
     8101 gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa
     8161 aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa
     8221 accttgtc
//
LOCUS       DACECY010000095         8193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.100, whole genome shotgun sequence.
ACCESSION   DACECY010000095 DACECY010000000
VERSION     DACECY010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.100"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..197
                     /locus_tag="IWO38_002548"
     CDS             <1..197
                     /locus_tag="IWO38_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314573.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="IWO38_002549"
     CDS             314..811
                     /locus_tag="IWO38_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0314574.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="IWO38_002550"
     CDS             898..1290
                     /locus_tag="IWO38_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0314575.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..3721
                     /locus_tag="IWO38_002551"
     CDS             1274..3721
                     /locus_tag="IWO38_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0314576.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            3724..5907
                     /locus_tag="IWO38_002552"
     CDS             3724..5907
                     /locus_tag="IWO38_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0314577.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            5904..6905
                     /locus_tag="IWO38_002553"
     CDS             5904..6905
                     /locus_tag="IWO38_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0314578.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            6920..7834
                     /locus_tag="IWO38_002554"
     CDS             6920..7834
                     /locus_tag="IWO38_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0314579.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
BASE COUNT         2551 a         1560 c         1743 g         2339 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagcag aacgtggcag atggaaagaa accttgccag agatttccca
     2821 tgaaatcaat atcgtcaatc tgacttctga tgagaaaaac aaaggcttac ttgaccctta
     2881 tgtaattatg aaaaatacca aagattctga atcactggct attgatattt tgacattcct
     2941 tacggggatt tcctctcgtg atggggaacg cttcccaatc cttagaaaag ccattcgtgc
     3001 agtaaccaat agtgaagtgc gagggttgat gaaagtgatt gaagaattac gggttgagaa
     3061 tacgccacta agtaccagca tagccgacca tatcgaaagt tttacagact atgactttgc
     3121 ccatctgctt tttagtgatg gttatgtaga gcagtctatc agccttgaaa aacaactgaa
     3181 cattatacag gttgccgact tggtacttcc tgacaaggaa acttcctttg aggaatatac
     3241 cacaatggag ttactatcgg tagcaatgct gattgtcatt agtacctttg cgttggactt
     3301 tatccataca gaccgaagta ttttcaagat tgtagactta gacgaagcat ggagcttctt
     3361 acaggtggca caagggaaaa cactatctat gaagctggtt cgtgctggtc gtgctatgaa
     3421 cgctggggta tatttcgtga cccaaaatac agatgacctc ttagatgaaa aactgaaaaa
     3481 taacctcggc ttaaaatttg cctttcgttc cactgacctt aacgagatta aaaaaaccct
     3541 tgcctttttt ggtgtagacc cagaggacga aaacaatcag aagcgattgc gtgatttgga
     3601 aaacgggcaa tgccttatca gtgatttata tggtcgtgtc ggtgtgatac agttccaccc
     3661 tgtatttgaa gaactgctcc atgcctttga taccagacca cctgtgcgaa aagaggtgta
     3721 aatgtgaaac catcaatatt aaacagaata aaatcaaact ggacgctgaa acgtctaggt
     3781 aaagtggcaa tgacagtggc tttcacactt gtgattgcca tttttctttt agccatgctg
     3841 ggaacggtgg ttcaagctgc gggcttggta gatgatacgg tcaatgtggc aaatgaatac
     3901 agccgatacc cacttgaaaa ctatcaactg gatttttatg tggataatag ctggggctgg
     3961 ctaccgtgga actggtcgga cggaattgga aaacaagtca tgtatggact atatgccatt
     4021 accaatttta tttggacaat cagtctttat gtttccaatg cgacgggtta cttagtacag
     4081 gaagcctatt ccttagactt catttccgct acagcagatt ccattggcaa gaatatgcag
     4141 acccttgcag gggttacgcc tagtggacta tcaacagagg gtttctatgt tggattcctt
     4201 ttactcttga ttttggttct tggggtttat gttgcctata caggactgat aaagagagaa
     4261 accacaaagg caattcatgc cctcatgaat tttgtgctgg tttttatcct atcggcttcc
     4321 tttattgcct acgctcccga ctacattaaa aaaatcaatg acttttcatc agacatcagc
     4381 aacgctagtt tgtcactggg tacaaaaatt gtcatgcccc attcagatag tcaaggcaag
     4441 gacagtgtgg acttgatacg tgacagccta ttttccatac aggttcagca accgtggcta
     4501 ctgcttcaat ataacagttc agacattgaa agtattggta ttgaccgtgt ggaaagcctg
     4561 ctctcaacca gtccagattc caacaatggg gaagacagag aaaaaattgt tgcggaagaa
     4621 attgaagata gaagcaatac caatatgacg attacaaaga cgattaaccg tttaggtaca
     4681 gtcttcttcc tatttgtctt caatattggg atttccatat ttgtattcct attaacagga
     4741 atcatgattt tctcgcaggt actttttatc atctatgcta tgtttctgcc tgtgagcttt
     4801 attttaagca tgattccatc atttgatggt atgtcaaaac gagccataac aaagctcttt
     4861 aataccattt tgacacgagc tggaatcaca ttgattatta cgacagcatt tagtatttca
     4921 accatgctct ataccttatc ggctggttat ccgttctttt tgattgcttt tctacagatt
     4981 gtgacctttg caggaatcta ctttaagctg ggcgatttaa tgagtatgtt ttctctacag
     5041 agtaacgatt ctcaaagtgt gggaagtcgt atgatgagaa aacctcgtat gcttatgcac
     5101 gctcacatgc accgtctaca gcggaaactt ggacgctccg tgactgcttt aggggctggg
     5161 tctgccattg ccagtgctac gggtaaaaaa ggacagtcgg gttcgggaag ttctgcgagt
     5221 acacaagcag atcactctcg accaaacggg caggaaaaat caacactagg aaagcgtatc
     5281 ggtcaaacca tcggtacagt agctgatacc aaagacagaa tggtagatac tgctggtaat
     5341 ttgaaagaac aggttaagga tttgccgacc aatgcaagat atgcagtata tcaaggaaaa
     5401 tctaaagtga cggataatgt ccatgattta accagaagta tttctcaaac caaagcggat
     5461 aaagccagtg gacgcaagaa acagcaggaa caaagacgga aaaccattgc ggagcgtcgc
     5521 tctgaaatgg aacaggtcaa acagaaaaaa cagccagctt cttccgttca tgaaagacca
     5581 gccactaagc aagaacaatc tcatgatgga cagacttcaa ggcaaacaac tgtacaggct
     5641 tcctataggg aatctcaaca agccaaacaa gagcgtccaa cagttaagtc cgattcttca
     5701 agtttaaaaa cggaacgtca aagtaataca gttcaagaaa gaacggttca aaagccagta
     5761 acttcaactg caccaacaga tagagcttca caacgttcaa tcacaaaaga acgtcagtct
     5821 actgttcaaa gagtaccact acaaaataca aaaagtagac caccaatcaa aaccgccacc
     5881 attaagaaag gcagtaagaa accatgaagc tgaaaacttt agtgattggt ggttcgggat
     5941 tattcttgat ggtcttctca ctgcttctgt ttgtcgccat tttattttca gatgaacaag
     6001 atggcggttt ttccaacatt cactatggcg gtgtggatgt ttccgcagaa gtgctggctc
     6061 ataagcctat ggtagaaaaa gttgccaaag aatatggaat tgaagaatac gtcaatatac
     6121 ttcttgcgat tatacaggtg gaatcgggtg gtacagcaga agatgttatg cagtcctcgg
     6181 aatccctcgg tcttccacct aattcattga gtacagaaga atccattaag caaggtgtga
     6241 agtatttcag tgagttatta gccagtagcg aaaggcttgg tgtagattta gaatcggtta
     6301 tccagtccta caattatggt ggtggtttct taggatatgt ggctaatcgt gggaataaat
     6361 atacctttga actggctcaa agtttctcaa aagagtattc gggtgacgaa aaagtgtctt
     6421 accccaatcc catagccata cctatcaatg ggggctggcg atacaattac gggaatatgt
     6481 tttatgtgca actggtaacg cagtatcttg tcacaacaga gtttgatgat gatacagtac
     6541 aagccatcat ggacgaagct ctgaaatttg aaggctggaa gtatgtctat ggcggagctt
     6601 ccccgactac ctcttttgat tgtagcggac tgacacaatg gacgtatgga aaagctggaa
     6661 tcaacttacc acgaacagca caacagcaat atgatgtaac ccggcatatc ccattattgg
     6721 aagcaaaagc cggcgatttg gttttctttc attctaccta taacgctggc tcttatatta
     6781 ctcatatcgg gatttattta ggcgataacc gtatgtttca tgcaggcgac cctatcggtt
     6841 atgccgactt aacaagctct tactggcaac agcatttagt gggagcagga cgaatcaaac
     6901 aataagaaag gacgattcaa tgatgaaatt cggaaaaaat cagaatacag aaaaacagac
     6961 accaaaggaa aagaaacctc gtgtctataa ggtcaatcct cgtaaaaagg ttgtgattgt
     7021 cctatgggta cttttaggac ttagtttcag ctttgcgata ttcaagcact ttacagccat
     7081 tgatacccat accattcatg aaaccaccat catagaaaag gaatatgtcg atactcatca
     7141 tgtggaaaat tttgtagaga actttgcgaa agtctactat tcatgggaac taaacgacaa
     7201 gtccattgat aatcgcatag aaaacctgaa agcatatctg acagaggaac ttcaagctct
     7261 gaatgtcgat acagttcgta aagatattcc tgtatcgtct tctgtaaaag gatttcagat
     7321 atggacggta gaagcagatg gcgacaatca atttgatgta acctacagtg tagaccaact
     7381 cattacagag ggggaaaata caaagaccgt ccactctgct tatatagtga gtgtctatgt
     7441 agacagtact ggaaatatgg tcctcattaa gaatccgacc attactagca taccaaagaa
     7501 atcagactat aaaccaaaag ctattgaaag tgagggtacg gttgattcca ttacaaccaa
     7561 tgaaatcaat gagtttttaa cgacgttctt caagctctat cctacagcga cagccagtga
     7621 actttcctac tatgtgaatg acgggatatt aaaaccaatc ggaaaagagt acatctttca
     7681 agaactggta aatcctattt acaatcgtaa ggataatcaa gtcacggtat cgctgacagt
     7741 ggagtatatc gaccagcaga ccaaagcaac gcaggtatct caatttgatt tggtacttga
     7801 aaagaacggg agtaattgga agattataga ataacaaata ttggtacatt attacagcta
     7861 ttttgtaatc acgtactctc tttgataaaa aattggagat tcctttacaa atatgctctt
     7921 acgtgctatt atttaagtat ctatttaaaa ggagttaata aatatgcggc aaggtattct
     7981 taaataaact gtcaatttga tagtgggaac aaataattgg atgtcctttt ttaggagggc
     8041 ttagtttttt gcacccagtt taagaatacc tttatcatgt gattctaaag tatccggaga
     8101 atatctgtat gctttgtatg cccttttggg cttttgaatg gaggaaaatc acatgaaaat
     8161 tattaatatt ggagttttag ctcatgttga tgc
//
LOCUS       DACECY010000096         8066 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.101, whole genome shotgun sequence.
ACCESSION   DACECY010000096 DACECY010000000
VERSION     DACECY010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.101"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..883
                     /locus_tag="IWO38_002555"
     CDS             <1..883
                     /locus_tag="IWO38_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ0314580.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            931..3060
                     /locus_tag="IWO38_002556"
     CDS             931..3060
                     /locus_tag="IWO38_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ0314581.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIESVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3035..3724
                     /locus_tag="IWO38_002557"
     CDS             3035..3724
                     /locus_tag="IWO38_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314582.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4132..4818)
                     /locus_tag="IWO38_002558"
     CDS             complement(4132..4818)
                     /locus_tag="IWO38_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0314583.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(4954..5148)
                     /locus_tag="IWO38_002559"
     CDS             complement(4954..5148)
                     /locus_tag="IWO38_002559"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314584.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5198..5638)
                     /locus_tag="IWO38_002560"
     CDS             complement(5198..5638)
                     /locus_tag="IWO38_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ0314585.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5642..6487)
                     /locus_tag="IWO38_002561"
     CDS             complement(5642..6487)
                     /locus_tag="IWO38_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ0314586.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6636..8054
                     /gene="pepV"
                     /locus_tag="IWO38_002562"
     CDS             6636..8054
                     /gene="pepV"
                     /locus_tag="IWO38_002562"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ0314587.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         2480 a         1514 c         1713 g         2359 t
ORIGIN      
        1 tatttcagta ccggttgcac tgttctcatt tatgggctcg attatggctg gaatctttgc
       61 taatatcgtc aatttcaaac aatatcaaac gatttatgct ggtttcaatc cagcggctga
      121 aagctatact tataatgact atttccaaat tggtatgatt gggatgattg taagcttagt
      181 tgtggtacta acggtcgcta atatatcaat gaacaaaaag aaacgctatg caatggcagc
      241 aaacgtacca gctgaaggtg gcgacgcacc aatgatctca tggttagccg tattattacc
      301 agttttaggg gtcgttcttt tagatcttca aatcatctta ggttttattc tagcaggtat
      361 ctgggcattg ctgtttactg gaaaattgcg cggaggttat aaagaaatct gtcgtcagtt
      421 tgcgaaatta tttacagatg gcgcagtcga tgttgcacca atggttggtt tcttgatgac
      481 cttagcgatg ttcaataatt cagctgcgta tgcttctcct tatttttcag ctatttttgg
      541 agattggata ccgcaatcac cattagtctt ggcaatcgta tttgctatat tgacaccact
      601 aggatttttc cgcggtccaa tgaatctagt cggttcaggc tcagctattt tagctgtcgt
      661 gttagctgtt aatccaacga tgtcgccagc ctttttgttc ccgttatttg caattacaac
      721 gattgctcct cagcatttag atattacaca atcttgggtt gcttggggat taggttatac
      781 aaaagtaact tctcgagaat atatgaagaa atccatccca actggttgga tcatcggtgc
      841 gatcttatgc ctgatcacgt tcttattgta cgggaacgcc tagtaaacaa aacgtcgata
      901 aaaacgatta gataaatgga ggaaaagaaa atgaaacgtt ttgtgcgaat gggaattgat
      961 gttggcggta cacatacgaa agcagtcgca atcgataatg cgacgcacga aatcatcgga
     1021 aaatcttcag tgaaaacaac ccacgatgat gtccgtggag tagctgcagg tgtcgttcag
     1081 tcttttcaaa attgtttgcg ggaaaataat atcagtccag aagatgttgt gtttgttgca
     1141 cattctacca cacaagcgac taatgcactg attgaaggag acgtagcaaa agtcggcgta
     1201 atcgggatgg caaaaggtgg attagaaggt tttttagcaa aacgacaaac tagattgaac
     1261 gatatcgatt tagggaataa gaaaaaaata gagattgtca atgcattctt acctgtcaaa
     1321 aacttaaatg tagaccgtgt ttcagaaacg atttcttctt tagaaagaga aagagcggag
     1381 gtcttggttt cttctatggc atttggagta gataatgggg aaccagaacg agttgtttat
     1441 gaagcagcat ctgtgaaagc gatccctaca acgatggctt ctgacatcac aaaattatat
     1501 ggattgacaa gaagaactcg gacagcagca atcaacgcat ctattttacc aaaaatgcta
     1561 gatacggcaa cctcgacaga agactccgta cgagaagcag gggtcaatgt ttctttgatg
     1621 attatgcgcg gagacggcgg agtaatggaa atcaatgaaa tgaaaaaacg acctgtttta
     1681 accatgcttt ccggtcctgc tgcatcagtc atgggctctt tgatgtactt gagagcgtct
     1741 aatggtgttt attttgaagt aggcggaaca acaaccaata tcggggtcat taaaaatgga
     1801 cgtccagcaa tcgattattc aattgtaggt ggccacccaa catatatctc ttcattagat
     1861 gttcgggttc taggtgtggc aggcggttcg atggtacggg ctaatcagtc aggaatcatc
     1921 gatgtcggtc cacgttctgc acatattgcc ggtctagatt atgcagtatt tacggagaca
     1981 gaaaaaatca aaggtcctaa ggttgaattt ttctctccta aagaaggaga ccctgctgat
     2041 tacgtcaagg tagtgatgga agacggagaa gaagtgacta ttacaaatac atgtgcagcc
     2101 aatgttcttg gattagtgca agaagaacat ttttcttatg gaaatgtccc atctgcacga
     2161 aaagccatac aggcattagc agactattgt catacgacgg ttgaagacat cgcagagcag
     2221 atcatggaaa aatcctatgc taagattgaa tcggtgattt tagaattggc agacaagtat
     2281 cacttggaga aagatcagat ttctcttgta ggtgtagggg gcggcgctgc ctcattgatt
     2341 acttacttta gtaataaaat gggtgtgaaa tattcgattc ctgaaaacgc cgaagtgatt
     2401 tcttctattg gtgtagcact tgcaatggtc cgagatgttg tagaacgtat catcccttcg
     2461 ccaagtaaag aagatatacg atcgttgaaa aatgaagcga tgaataaagc gattgaatca
     2521 ggcgctacac cagaatcgat cgaagtccac gtagaaatcg atccacatac ttcaaaagta
     2581 acggctatcg ctactggttc tacggaagtg aaagcaaccg atctaaccaa ggagatcaca
     2641 acagaagaag cactagagct tgctgcagag gatatgcgat taaataaaaa tgaagtttgc
     2701 ttattggaaa atacaccttt cttttatgta tgcggcgaac agaatcgttc aaaaaatgct
     2761 ggaagtcttc ggatcatcga tcaaaaagga tttatcaagg tccaacgtgg acatgcttca
     2821 tgtatgaaaa caactgcagc caattatatg acagctgtcg aacaactttg ggaagatatg
     2881 gcagtttatc aaactgaatt gattgcccga ccagaatttt atctctgctt aggagcaaga
     2941 gtctctgatt ttacagcgac ggatctggaa caactacagc ttttgatgga tctggaagta
     3001 tctactatgg aaccggagga agaagtgatc gtcgttgccg gaaatatcaa acaaacataa
     3061 gttagaagag ttactaaacg aattacaaca gatccccgaa gaaatttggg gattctacca
     3121 gtttcggcga gatttatttt ggaaaaagat tcctttgtcc aagcagaaaa ttcttataca
     3181 acaatcaatc gattgtggta tcgaaacggc atgtagcatc aaaaaaaagt acccttttgc
     3241 cgatgtagga gaaatctgtg aacaaatggc tgttccaatc gtttcctgtg aatcagagca
     3301 aataaacgaa cgaatcactt ttgcaactta tgcagaagat gaagggatcc gattgatgac
     3361 ggagccgtta gaaaaattaa aatgttcagg actagcttcg atctctaaag aaacagcgca
     3421 agctttgatt attggacatg aactatttca ccacatcgaa gcttctgtta agggaattta
     3481 tacgcaaagc gaaaaaattg tgctgtggcg gttgcctttt tatacacatc aatcaaacat
     3541 tcgtgcattg agcgagatag cagcgatgag tttttcaaaa gagatgaatc aaagcgattt
     3601 ttcgccttat gtattagagg cagtcttact ttggccatat aatgaaattc gtagccaagg
     3661 gatcttagaa gaaataaaag aaatagaaaa aagatgtacg gagtatgatt tagattacaa
     3721 ataaagagac tgtgacaaaa gtcgtgtcac agtccttttt ttccgaataa acggtgttct
     3781 cctgtcaact cctcggtcac aagtcaaaat attcaacaaa catgagaagc tgttttttga
     3841 aattgttcct tgtgactcgg agatgaacgg caggctcacg acctcttgga aaaaggtgtt
     3901 caaaagcttg catctgcggt aataagaaca gccaagttaa aaacataaga agctattttt
     3961 acttggctgt tcttattact tgatgcaggg cagctttttc acaacctctc aataaacggt
     4021 gtgcaatcag ctgaccctcg gtattagctc aaaaaaaacg gcaaaatatg aggaactatt
     4081 ttgccgttta tttctctaat actcaggtca aaacgctgat tccaaaacct cttatttgat
     4141 gggtctgtcc tgaatatctt caaaataatc ggggtaatta tgaatcacgt attcttgctc
     4201 ctcgataatc atatgtaaat gcatcgtgca aagaaatccg acttcatcaa agttatgatc
     4261 aatcattgat tgaagcattg tctgatgttc atctaatact cgaccccagt ctaattctga
     4321 aatcgtcaaa cgtaaccatc tgaaacggtt caagtgcgta ctataacttt ctagccaatc
     4381 ccaaatttct cgttttccag caatttcata acaagttcgg tgaaaatcag tatccaaatc
     4441 aaaaaaagct tttttatttt gagcgatcac tgcctgatcg gtttgttcta gtattttttc
     4501 tagtttagct tgatcttgct tggtcatttt cgcactgcat tcttgcagga tcggtttttc
     4561 caacttttcc cgcgtataac gggctaatga agcagatcgg atatcgattt ttgaaacata
     4621 agtgccggac tgtggaatgg tttttacaag ctcttgattg cgcagttgga tcagtgcttc
     4681 tcgaatcggt gtacgcccga tagctagttg ttcttctaac actttatcgg agattttact
     4741 tccgggtgct aactcggaat aaatgatttg tttacgtata gattcataag cttgctgttg
     4801 caaattattg ataaccattt aaccttcctt tctatgcaga tataatctat tataacataa
     4861 atcgcttaca aatcgtgtgg gcataaaaac acagtctgca aaaaaagccc tgataggcat
     4921 gcctattaga gcctcgaaaa gaaaaatatt gaatcaatct ggtcgtacct cttggatcgg
     4981 tttgacacga ccgccgattc ttttagcaaa agcttcggat tcttttttgt ctttgaaaat
     5041 gagcagattg tctaagtttt tttcccgagt gcgacagcca gttttatcga tgtaaccgtg
     5101 cattacttta acgacataca tggggatcac ctgctttttt cttttcatta tacgaactac
     5161 tcgacacaat acaagaaaaa tgcttagacg gtctttatta agaatttaca tcatccaaag
     5221 aagcgtacgc ttggaataac tgttctaact ccggattgac tttgctcagc ttcactaatc
     5281 gttgcgaatc agaaagtaaa aaacagtgtt tactactgcc agtagttttt aattttttgt
     5341 cttcagttcc cacaatttcg tagctaaaat ccaaccgtgt tcccgtatac ttttggatag
     5401 ttgggtggat gctcactttt tctccgtaat ggatcatttc tttatagctg caatttactt
     5461 ccagaacggg gatgatgatc ccagattctt cgattttttt atagggaaac cctagatgta
     5521 ccaataaggc tacccgagcc tcttcaaacc agcggatata attgctgtga tgaataatcc
     5581 ccatttgatc tgtttcataa taaaaaggtt ctcgcacata acctgtaaat ttctccatga
     5641 ctcatgcctc gttttccatt gattttttac aaaaataatt catccacttc ggaagtgctt
     5701 cgctgatttg tgtcggcaaa acaatatatt gttcttttgc cagctggtct ccaatgaaac
     5761 tatgaagata aacagctgct gcaatcgttt ggtcattttg aggaaactgg gctaaaaatc
     5821 ctgtgatcat cccagctaaa gtgtcaccca tgccgcctgt tgccatagca gcagaaccta
     5881 aaggattggt gtagaagtgc tggtgaccat aaatttttgt ctcatgactt ttcaaaacga
     5941 gcacagcacc gatttcctgt tgcttctttt tgttttcttt atcccgttgt tcttgtatcg
     6001 gtagagaact caaccgttgc cactccattt gatgaggtgt aagtacagtt tgttcgggat
     6061 agggtagcaa aagatgatta gcaacaagta aacttaaagc agagccatca atcaccagcc
     6121 attgttcttt tgattggttt tctagtactt gtttgaaacg ttccaaactc ttagaagaag
     6181 tacccaatcc gggaccaatc aagagtacat ctgcttgctt tattacggaa agaaactggt
     6241 cagtatccgt ccaatctaca atcattgctt ctggcagatg actgtgaaga gcactccgat
     6301 ttttttcatc agttactaca gtcgtgagac ccgctccact gtgtacggca gctaaagcac
     6361 tcataatgat tgctccgcca tattgttcat tgccgccaat caaaacgata cgtccaaatg
     6421 ttcctttatg tgaaatcttt ggacgaggtg tgatgactct tttcactagt tgttcgttta
     6481 tcagcatcga aaaacctcct tttactcatt atagcgtata ttttttttag agaaaaacag
     6541 ataagaaaag gaattgtcag attttttgaa ctattttttt ctgtaatttg tggtatcatg
     6601 aacacgaaga cttaaataag tggaggatta actttatgac aattgattgg caaaaagaag
     6661 tagaagcacg taaagaagac ttgcttgaag acttgaaaaa tcttttacgt gtcaacagcg
     6721 agcgcgatga ttcaaaagta acaccagatg caccatttgg acctggacct cgcgacgcat
     6781 tgaaacatat gttagcttat ggcgaacgtg atggatttaa agtaaaaaat gtcgataact
     6841 atgcagggca tattgatctt ggcgaaggag acgaaacgct agggatattt ggacacatgg
     6901 atgtcgttcc tgcaggagat ggctgggaca cagatcctta tgaaccagta atcaaggatg
     6961 gaaagatctt tgcacgcgga tcaagcgatg acaaaggtcc aagtatggca gcttactatg
     7021 cgatgaagat cattaaagaa cttgacttga aactatcaaa aaaagttcgc ttcgtagtcg
     7081 gcagcgatga agaaagcggc tggggcgata tggcttatta tttcgaacat gaagaagaac
     7141 cagattttgg tttctcacca gatgctgaat tcccaatcat caatggtgaa aaaggaaatg
     7201 tttctttagc tttacgcttc aaaggcgaca atgcaggaga ttatgtcttg aaatcttttg
     7261 tttccggact tcgcgaaaat atggtgccag gaacagcaac tgcagcactt caagtaccaa
     7321 gtgcagatgc agctatcgcg atggaagaag cattttacca attcatcgaa gcaaatccag
     7381 taagcggaac gattgaagcg gacaatacgt atgtaaaaat cgaattagtt ggtaaaggcg
     7441 cacatggtgc aagcccacaa tctggtatca atgcaggttc tttcttggca ttgttcttag
     7501 ataactatga attcctagga tctgctaaac aatttatcca tgtagcagct gcttatgtcc
     7561 atgaagattt ttatggagaa aaactaggag tcgcttatga agatgaaaaa atgggcaaat
     7621 tgacaatgaa tgctggacta tttgcttttg aagaaaatgg tacagaggaa gcgaacttca
     7681 tcaatatgaa tttccgtttt ccaaaaggtg taacagtaga cggattgcaa agtgacatcg
     7741 aacaaacagt ggggcaagaa ggcgcaacag ttactcgagg tgctcgcgta atggaaccac
     7801 attacgtacc aatggatgat ccattggtgg ctacattatt gcaagtctat gaagaccaca
     7861 ctggagaaaa aggctacgaa caaatcatcg gtggcggaac ttacggacgt ttgttgaaac
     7921 gtggcgttgc ttatggagcg atgttcccag gatatactga tacaatgcat caagcaaacg
     7981 aatttatgag tcttgatgac ttgttcagag caacagcaat ctatgcagat gccatctatc
     8041 gtttggcgaa ataaaaatac taaaaa
//
LOCUS       DACECY010000097         7637 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.103, whole genome shotgun sequence.
ACCESSION   DACECY010000097 DACECY010000000
VERSION     DACECY010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.103"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            344..3178
                     /locus_tag="IWO38_002563"
     CDS             344..3178
                     /locus_tag="IWO38_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0314588.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLGTFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            3369..3860
                     /locus_tag="IWO38_002564"
     CDS             3369..3860
                     /locus_tag="IWO38_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ0314589.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            3963..4952
                     /gene="manX"
                     /locus_tag="IWO38_002565"
     CDS             3963..4952
                     /gene="manX"
                     /locus_tag="IWO38_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ0314590.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            4985..5788
                     /locus_tag="IWO38_002566"
     CDS             4985..5788
                     /locus_tag="IWO38_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ0314591.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5809..6723
                     /locus_tag="IWO38_002567"
     CDS             5809..6723
                     /locus_tag="IWO38_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ0314592.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6806..7180
                     /locus_tag="IWO38_002568"
     CDS             6806..7180
                     /locus_tag="IWO38_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ0314593.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7319..7564
                     /locus_tag="IWO38_002569"
     CDS             7319..7564
                     /locus_tag="IWO38_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0314594.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
BASE COUNT         2475 a         1263 c         1683 g         2216 t
ORIGIN      
        1 ataaaagttg tagtggaggc tacgaagttt actttgtctt tttacactta atattttttt
       61 gccaatttgg aatcaggatg ttttgataac tatcgttttc aattgatgga agctatattg
      121 tttgctatca gtgaaataat tatatggtaa acagtgttct ttttcttgtt tccattataa
      181 aaatcgtatc gggatttttc gttcttttaa attgaacatt tctatctatc cgcgtgttat
      241 caacaaaaca aaatcaggta aatagttgac gaaacagaaa taaatacata gtattatgtt
      301 ttgaagccta agtacaatag tctatagaaa aggagcgata ccattggcaa aacgcattga
      361 ccgaatttat atgtatgtca aagaaaaaac cgctcattta acaaaggcag aatatgatca
      421 aggagtaaca acacaggaaa ttgctgaagt gttagggatt cagcgaacaa atagcagtaa
      481 agatctgaat cagctagtgc gcgaaggaaa actgataaag acagacggac gaccagtacg
      541 ttacatatat caaacactcg ttacgcacgt aaagccagtt acaaaacatg tagtcagtta
      601 caaagaaacg ccggtctctg aagaaaaaaa tacactccaa atagatacta aggatatctt
      661 tgcaaaaatc attggagcta acggcagcat gaaaaattca gtggaacagg caaaagctgc
      721 gattttgtat ccgccaagag gattaaattg tttgattaca ggacctaccg gatcaggaaa
      781 gacttatttt gcacacgcga tgtatcattt tgcaaaagcg catcatgtaa tagatgagga
      841 aacggaactg attgttttca attgtgctga ttatgccaat aaccctgagc ttttgatgag
      901 tcatttattt gggtacgcaa aaggtgcttt tacaggcgca gatgaagaaa aaatggggat
      961 tatcgatcaa gcggatggcg gtatgctgtt tttggatgag attcaccggt tgccgcctga
     1021 aggtcaagaa atgatctttt actttatgga tcatggaact tacagtcgtc ttggggaaac
     1081 aacgaaatcc catgaagcaa atgtacggat cgtaggtgcg accacagaag acccaggttc
     1141 ttctctatta ggaacttttg ttcggcgtat cccaatcaat atcaaacttc cggcttttga
     1201 aaaacgtcct gcaaatgaga aagtcgattt agtaaaaatc atgattgccc acgaagcaaa
     1261 ccgcacgcag cgaaagattt ctttgacaga agatgtagca aaagcattga tcggcagtgt
     1321 gacctatgga aatataggac aattgaagtc aaatatccag ttagtatgtg cacgaggatt
     1381 tctaaatcat atgcaatcac ccgaaatctc gatcacaatc gatgatttga cagaagggat
     1441 tcgtagcgga ttgattcaat tagcaagcaa ccgcagtgca atggcagagc tttctaaaat
     1501 attggagcca aagataactg tagcaccaaa tgatacgatc atgaaaatcc aatcggactc
     1561 ctatgagtta ccttataatc tatatgatat catcggagat aaagcagctt tactgaagtc
     1621 ggatggtttg gatcaagaag caatcaatca tttcatttct acagatatca acatccatct
     1681 gaagtctttc tataaagacc atggtttttc ttttaatgca gacagcaaat tagcagaatt
     1741 tgtagatccg aaaatcatag aagtgaccaa tcagatttat gtaatggtca aaagatcttt
     1801 accatatgag tttcagcaaa actttattta tgctatgagc ttgcatatca gttctttttt
     1861 gaaaaaaatc aacttaggag aagaacggca tacgaatgat aacatccgtg agatggcttt
     1921 tgattttcca gatgaatatg ctgttgcgaa agaagtgcgt agttatatcg aaacgtattt
     1981 tcaagtggag attccagaaa gtgaagacta ttacttgacc gttctactcg tctcgttacg
     2041 ggcaaatcaa gcaagcggca gaatcggtgt agtcgttgca gctcacggaa acagcacagc
     2101 aagcagtatg gtccaagttg tccaacagtt gttagatgca gagaacgtac gagcagtaga
     2161 catgcctttg gatatggatc cgaaaacagc attggaccga attgaacgca atgtacaaga
     2221 agtagatgaa ggaagcgggg tcatactctt agtagatatg gggtcccttg cgtcgtttaa
     2281 ttcgcaaatt caaagagaca ctggaattcc agtacgtact gttgatatgg taacaacttc
     2341 tctagttttg gaaactgtac gaaaggtctc agtattaggg acagatctag atatgttgca
     2401 tgaatcattg aaaaattttc gaggttacgc agaaatatct tctgtagaat ctgttcagga
     2461 agcaaaacct attcataaga aaaaagctgt tttggctatc tgcgcatctg gcgaaggtac
     2521 agcacaaaga atcaaagaat tgatcgaacg agcggtaaac aagcgtcaag aaacagaatt
     2581 agaagttcta gctttgtcga ttgtggaagt gaaagaagaa ctgccagtta tccaaaaaga
     2641 atatcagatc atagctacta ctgggattac tgatttgaat atccaagccc catttattcc
     2701 tttagaacgt tttattgatc aaaatatcga agtcattttg gatcagttgc tgatggaatc
     2761 agaattggag gaaactgaat tcatctccct agatgaagag tcagctaaaa atacgtgtgt
     2821 tgagtttatc tctgataact tcatctttat taatggtagt aagttaatcg atccaatgtg
     2881 gcaattttct acacaaatct ctcagacgac gggaatcggc gatgaagaat atggattcaa
     2941 aatcaaccta gtgatgcaca cagcaggaat gatcgaacgt attattcgga atgaaccatt
     3001 gactgtggaa gaaaacgaat tgacaaatac aacgaatgat cctctttata gccaattggc
     3061 agcctcagtt gttctgttgg aagatcagat aaaagtaaaa gtgccgatag aagaaatgta
     3121 ttatctgcta cgtctagtcc ataatcagtt agataaaaaa gaatacactg tgccatgaat
     3181 acacaaaaac gatacacttt tatagtgtat cgtttttgtg tattggttaa gaatccttta
     3241 aattcaatta gtgtatcgta aaaaagttgg cacgcttttt gcttatagta gtgtgtgcta
     3301 ggaggtggtt gttcacacag aaatgatagg ggtttctgtg aagtagtgag gaagaaggag
     3361 ttttgcagat ggatattcga ttggcacgta tagatgatcg tttaatccac gggcaagtcg
     3421 caacagtttg gtcgaagcaa acaggtgttg aacgtatcat tgtgatcagc gatgtagtcg
     3481 ctcaagataa attgagaaag tttctactaa aagaagctgc accgccgggg atcacagcaa
     3541 atgtaataac cgtgcagaaa atgattcaaa tctatcatag tggattatta tcacgagcaa
     3601 aagtgatgct tttattcacc aatccccaag atgttgaagc ggttgtccgc tgcggtgtac
     3661 agttgaagtc gttgaatatc ggcggtatga gttttactga aggcaaatca atgattacca
     3721 attttgtttc agtaaatcaa caagatattg attcctttcg ttacttgaac agtcaagaag
     3781 tggagttaga gattcgaaaa gtacctgctg acaggaaaat ccttttgatg gaactactag
     3841 cgaaaatcaa aaaggtgtag caagaatggt tgttggaaaa ggtcaaataa ttctgttgta
     3901 atggttagca attattaata tttttgaatt tgttacaaca aaagtaaata ggaggtataa
     3961 atatggtagg gattatccta gcaagtcatg gacaatttgc tgaaggaatc ttacaatccg
     4021 gttcaatgat ctttggcgaa caagaaaacg tcaaagccgt gattttgaaa ccaagtgaag
     4081 gtccagatga cttgagagcc aaattggaag aagcggttgc ttcttttgat aatcaagacg
     4141 aagtgttatt cttagttgat ttatggggcg gaacaccgtt caatcaatca aacacattat
     4201 tcgaagagca caaagataaa tgggcaatcg tcagcggttt gaatttacca atgttgattg
     4261 aagcctacgc atcacgcttt tcaatggaat ctgctcatga aattgcagca cacatcattg
     4321 aaacagcaaa agacggtgta aaagtaaaac cggaagaatt agaaccggca gaagcaccga
     4381 aagcagcagt tgaagacgca caacctaaag gagcacttcc agaaggaact gtggtcggag
     4441 acggaaaaat caaatatgta ttagcacgtg ttgactcacg tctgttacat ggacaagttg
     4501 cgacagcttg gacaaaagca gtgcaaccaa accgtattat cgttgtttca gatgcagtat
     4561 caaaggatga tctgcgtaag agactaatcg aacaagctgc tcctccaggt gtaaaagcaa
     4621 atgttattcc tattagcaaa atgatcgaag tagcaaaaga ccctcgtttt ggaaatacca
     4681 aagcattgct tttatttgaa aatcctgaag acgtattgac agctgttgaa ggtggcgtag
     4741 acatcaaaga attaaatgtt gggtcaatgg cgcattcggt cggtaaagtc gttgtaagta
     4801 aagtattgtc tatgggacaa gaagacgttg aagcgtttga gaagcttgaa caaaaaggtg
     4861 tgaaattcga tgtacgtaaa gtacctaatg attcacgcga taacatggat gatattttga
     4921 aaaaagcaaa agctgaatta gcaaaagcgt aataaataat attaaaattt ataggaggct
     4981 tatcatgtct attatttcaa taattttagt cgtattagtt gcctttctag ctggtatgga
     5041 aggtattctt gatgaatttc aatttcatca accactagtg gcatgtacat tgatcggttt
     5101 agttaccggt aacctcgaag ctggtatcgt acttggcggt acgcttcaga tgatcgcttt
     5161 aggttgggca aatatcggtg ccgctgtagc accagatgct gcattagcat cagtagcatc
     5221 agcaattatt ttagttttag gtggacaagg tgttaaaggt gttccttctg cgatcgcgat
     5281 tgccgttcct ttggctgttg cagggttgtt cttaacaatg atcgtacgta caatcgctgt
     5341 tccaatcgtt catttgatgg atgcggctgc tgaagaagga aacatccgta aggttgaaat
     5401 gtggcacatc attgctgtat gtttacaagg ggttcgtatc gcggtcccag cagcagcgtt
     5461 gctatttatc ccagcacaaa ctgtgcaatc attccttgaa tcaatgccag catggttaac
     5521 agatggtatg gctatcggtg ggggaatggt cgtagctgta ggttacgctt tggtaatcaa
     5581 catgatggct acaaaagaag tatggccatt cttcgttatt ggtttcgttg tagctgcaat
     5641 ttctcaatta acattgattg ctctaggtgc tttaggtgtt gcattagctc ttatttactt
     5701 gaacctttct aaaatgggtg gttcatcaaa taatggaggc ggaagcaact ctggtgaccc
     5761 tttaggcgac attttgaatg actattaatc ttgaaggagg aaaataaaat ggcagaagaa
     5821 aaaatcaaat tatcaaaaaa agatcgttta gctgttgcgt ggcgctctac cttcattcaa
     5881 ggttcatgga actacgaacg tatgcaaaac ggtggctggg cattttctat gattccagca
     5941 atcaaaaaat tatacaaaac aaaagaagaa cgttctgcag cattaaaacg tcacttggaa
     6001 ttctttaata cacacccata cattgcttct ccaattttag gggttacatt ggctctggaa
     6061 gaagaacgtg cgaatggcgc gcctgttgat gatgtagcga ttcaaggggt taaagtaggt
     6121 atgatgggtc ctttagctgg tgttggtgac ccagtgttct ggtttacagt acgtccaatg
     6181 ctaggtgcac taggtgcttc attagctatg ggaggaaata tccttggacc aatcatcttc
     6241 ttcttaggtt ggaacattat tcgttgggca ttcatgtggt atacacaaga atttggctac
     6301 aaagctggtt ctaaaattac tgatgacctt tctggcggat tgttacaaga cgtaacaaaa
     6361 ggtgcttcaa ttttagggat gttcgtatta gctgcccttg tacaaagatg ggtatcaatc
     6421 aaattcttac cagttgtatc aactgtaaaa ttagataaag gtgcttacat cgaatgggat
     6481 aaattgcctt caggtggtga aggaatcaag agtgcatttg aacaagttaa taatggctta
     6541 gccctttctc ctgaaaaggt aacaacatta caagacaact tagaccaatt gattcctggt
     6601 ttagctgcat taggattaac attgttatgt atgtggttgc ttaagaaaaa ggtttctcca
     6661 atcgttatca tccttggttt attcgtagtc ggtgttgttg gacacgtcat cggactttta
     6721 taatccataa aaatatagct cgggcgttgt cccgagcttt ttttatatag tatactgttc
     6781 ataaacaaat atctggaggg agaaaatggt tcaatcaatc aatacaaaag tagacttggt
     6841 aattgatgct acttcgttta cgggtctgac agattacggg caaatcatga taggtgataa
     6901 tggttttgag ttttaccatg cgcgagatag acgcaaatat atccaaattc catgggaaga
     6961 agtggattat gtactagcat cagtcatgct aaagggaaaa tggattcctc gatatgccat
     7021 tcaaacgaaa aaaaatggta catacacatt ttcttccaaa gaacctaaaa aagtgcttcg
     7081 tgcgatcaga gaatatgtag atgaagatag aatggtacaa tcattgagtt tctttgatgt
     7141 agtgaaacgt ggtattcgta gtatgtttaa acgcaagtaa aaaataaaga aaacttctaa
     7201 gaggaggttt tctttatttt tttactttgc agaagaaaat atgaaatagc aaaaccaatt
     7261 atttgtcccc tttaaatccc atatgctaaa attgattagc aaagaaggag gttatattat
     7321 gagttggtta tggacattga ttgttggagc cgtgatcgga gcaattgcag gtgcgatcac
     7381 cagtaaagga aattcgatgg gctgtgtaac gaatatcatt gctggactag tcggttcaag
     7441 tattggacaa gcgattttcg gcgaatgggg accaaaagct gcaggtatgg cacttattcc
     7501 atctatttta ggcgcagtga ttttggtagc agttgtctca tttttctttg gaaaacgatc
     7561 ataagttttt acaaaatagt aaactaaaag gagttgcagc gagactgtga ctcctttttg
     7621 gctttattta tttaaaa
//
LOCUS       DACECY010000098         7626 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.104, whole genome shotgun sequence.
ACCESSION   DACECY010000098 DACECY010000000
VERSION     DACECY010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.104"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(14..706)
                     /locus_tag="IWO38_002570"
     CDS             complement(14..706)
                     /locus_tag="IWO38_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0314595.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IWO38_002571"
                     /pseudo
     CDS             791..967
                     /locus_tag="IWO38_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IWO38_002572"
     CDS             complement(1143..1712)
                     /locus_tag="IWO38_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0314596.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IWO38_002573"
     CDS             complement(1786..2109)
                     /locus_tag="IWO38_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314597.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IWO38_002574"
     CDS             complement(2263..3075)
                     /locus_tag="IWO38_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314598.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="IWO38_002575"
     CDS             3429..4277
                     /locus_tag="IWO38_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ0314599.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="IWO38_002576"
     CDS             4422..5363
                     /locus_tag="IWO38_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ0314600.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="IWO38_002577"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="IWO38_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1512 c         1371 g         2471 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaaa
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag ttacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgagaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgaa tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaacgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tcgaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctt
//
LOCUS       DACECY010000099         7451 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.105, whole genome shotgun sequence.
ACCESSION   DACECY010000099 DACECY010000000
VERSION     DACECY010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.105"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            236..1627
                     /locus_tag="IWO38_002578"
     CDS             236..1627
                     /locus_tag="IWO38_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0314601.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAESAFPKEKTYR"
     gene            1665..2495
                     /locus_tag="IWO38_002579"
     CDS             1665..2495
                     /locus_tag="IWO38_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0314602.1"
                     /translation="MENKNISILLSLYDKEKPEYLKEALASIFEQTVMPAEIVLVYDG
                     PINEKLQAVVTYFQQKVPHLFTVVKLEKNQGLGIALATGLEHTKNRLVARMDTDDIME
                     PHRLEKQLEIFQEYPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFN
                     HMTVMFDKNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYAR
                     RGGSKYLLPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN
                     "
     gene            2467..3456
                     /locus_tag="IWO38_002580"
     CDS             2467..3456
                     /locus_tag="IWO38_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0314603.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLRRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNYGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNHPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQSKREIKSIYSKIRSNNEISIAKKLALFLYLINPKTYFILYKKVLKK"
     gene            3570..4565
                     /locus_tag="IWO38_002581"
     CDS             3570..4565
                     /locus_tag="IWO38_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0314604.1"
                     /translation="MHKISVIVPAYNVENYLENCLESISNQTHSNIEIIVVDDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILVFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSPKLKKLVKFLLINKNLPYYMSSSKIASKFLIKIESVR"
     gene            4569..5711
                     /locus_tag="IWO38_002582"
     CDS             4569..5711
                     /locus_tag="IWO38_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid biosynthesis protein F"
                     /protein_id="HAQ0314605.1"
                     /translation="MKNIKKFFSRNIFPVFIKILPIQKNLIVFSSFGGRNYSGNPRVI
                     SEKIHEKKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTI
                     KYAKRKKQFYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSEK
                     YVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQL
                     EWLGVDNLREFFKKQQQELNLMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLE
                     MLFEEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDS
                     YGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELLERDIK"
     gene            5708..7135
                     /locus_tag="IWO38_002583"
     CDS             5708..7135
                     /locus_tag="IWO38_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ0314606.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFLQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIAGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIILFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPLFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTALSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVVLFFIVNLTLTTSAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEVRKIIRK"
     gene            complement(7202..7366)
                     /locus_tag="IWO38_002584"
                     /pseudo
     CDS             complement(7202..7366)
                     /locus_tag="IWO38_002584"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2655 a          973 c         1311 g         2512 t
ORIGIN      
        1 tatgaatatt gtaacttgtg aataaggaat tagcaggaga atactagttc agcataaagg
       61 aagtgatggg acttctgaga caactttctg ttatgcagaa atacacatat aaacagacaa
      121 taaacgcttt tccccgtttc ttttaatttc ctttacaatt tggttattca ctaggttttt
      181 aaactttgct atggtaagat gatagaggca ttattttatg gttaggagtg tatatatgaa
      241 taaaaatggg gagtggaatg cggcacgcag aatattcatt attcttatgg atgtacttgt
      301 attaaatgta tcgatctatc ttagtttttt gctgaaattc cagggagaag taccagtgag
      361 aaacttagaa acctttcagc attcagcaat ttttatttcc atcatcttta ttgcgttgaa
      421 tattttgtta ggagcttatg tattctataa ccgaatgatc agtgatattg tcttcattac
      481 tgttattgga caagtactaa tagcattggg gatcatggta gttacttttg ctggccgatg
      541 gtttgcattt ccgcgtggcg tcattttgat ctcgtttatc ctaggtacga tattacttag
      601 tgtttaccgg atagtcgttt acaaactgta cttaagagtc agtcgtgata agaaagtcct
      661 tatcgtgggt ttggagaaag atgtcgcacc agctatccaa aattttcaat ctaagaaaaa
      721 caataaacat aaggttgaag cagtggcaat cgataattat tacgaaaata caaaaaaatt
      781 gatcgatgat atggatatcg tctatttggc ttctaaaatc ccagaagatg aaaaaatcaa
      841 catttatcaa ttagtgacta aaaaagagaa gaaattattt ttaaatacga cttttgagaa
      901 tctgacaatg ataaatccga atattatgaa ttttgaagat gaaagcatca ttgaagcttc
      961 tgggttcaga atccaaccag aatacgaact attcaagcgt ctctttgata tcatcgtttc
     1021 gcttatttta ctgatcctcg cctcaccttt tatgctaatc acagcgattt tggtaaaaac
     1081 aacttcacct ggaccaatca tctataaaca agtacgtatt acgaaaaatc aaaaggaatt
     1141 ttcaatctat aaattccgtt ccatgagtgc gacagctgaa gcaaaatcag gaccagtctt
     1201 ggctaaaagt aacgatgcac gtgtgacacc agtaggtaaa tttatccgtg ctgtacgttt
     1261 tgacgaatta cctcaaatct ttaatgtatt gaaaggcgat atgtcgattg tcggtccaag
     1321 gccagaaaga ccatttttcg ttgatcagtt caatgaagaa aatccttatt attacttaag
     1381 acacaatgta cgtgcgggaa ttaccggata tgcacaagta tacggaaaat atgtatcaga
     1441 ttataacagt aaactacgtt ttgatttgtt gtatatcaaa aagtattcct tactattaga
     1501 tttgaagatt ttacttcaaa caatcaaaat cttatttgat aagatgtcct cacaaggact
     1561 agatgaagat gaaaatatca gttcattagc agaaagtgca tttccaaaag aaaaaacgta
     1621 tcgttagatt ggaaaaattt tgagcaaaga agtaggagag aacattggaa aataaaaata
     1681 taagtatact gctttctttg tatgataaag aaaagccgga atatttaaag gaagcattag
     1741 ctagtatctt tgagcaaacg gtgatgccgg cagaaatcgt attggtttat gatggcccta
     1801 taaatgagaa gcttcaggca gttgtcactt atttccagca aaaagttcct cacttgttta
     1861 cagttgtaaa attagaaaaa aatcaaggat tggggattgc tttagcaact ggattagaac
     1921 atactaaaaa tcgcttagtg gccagaatgg atacagatga catcatggaa ccacacagat
     1981 tggaaaaaca attagagatc ttccaagaat atccagaaat agcgattgtt gggagtaata
     2041 ttgaagaatt tactggcaca cttagcaatg ttatcggaag aaggattgta cctgagtata
     2101 atgatgaaat ccgtgtgttt tcgaaaagaa gaaacccttt taatcatatg acagtcatgt
     2161 ttgataaaaa tgctatttta gaagtgggaa actatcgccc attaattgga tttgaagatt
     2221 attatttgtg ggtaagacta ttgaaggcgg gatataaagg atataatatc caagagattc
     2281 tagtacatgc tagagcaggt gctgacatgt atgctagacg tggaggaagt aaatatcttt
     2341 taccaggaat taaaggccga tatcgcgtat ggaaagatgg tctaggatca ctaaaagatt
     2401 ttctgatagt tatttgcgga catcttgtaa ttagtttatt accgaataaa attaggggaa
     2461 aagtatatga atcaaaactt agaaaaacaa actaaacttt ctataatcat acctttttac
     2521 aatgcaaaga atactttaag aagagctgta gacagcgtct taacgcaagg ttatcccaat
     2581 attgaaatct tgttaattga tgatggatct acagatggta gtagtgacat tggtgacgaa
     2641 attgctaatc tagagagtaa agtagcatgt tatcacttgg aaaacgctgg tccatcagtt
     2701 gctaggaact atgggataaa agaagcaact ggaaatttgg taggattttt agatagtgac
     2761 gattattttt tacctgattg ttttaataat gttttgaaag actaccatag gaatcacccc
     2821 gatatcattg cttttggatt aagtaaagga aaaaatatag aaaatgcaaa tttatttgtt
     2881 cccgaaaaga agattggttg ttcaagtgaa gaagcaattg agcaaatgtt tagaagtaag
     2941 gcagtggact tttacgtatg gaataaattt tttaaaaggg aattattcaa tcatatagtt
     3001 tttccagaaa ataaattata tgaggatatg gttcctatgt atgaagcttt tagggcggct
     3061 agtaaaattg atattttgcc attttcaggt attttttatt atcaaaataa cgatagcatc
     3121 gtttatcagt cttttaattc aaaacagtat gataatattt tgcagagaaa aatattagtt
     3181 gatgaagtaa agaaagatta tcctcgactt attaatttgg ccaatgctcg gttagtagac
     3241 ggatatcttt caacagggtt taaaattact ggcaaaaatc aaaatcagaa agaaaaacaa
     3301 aaatattata atcagtctaa aagagaaata aaaagtatat attcaaaaat aagaagtaac
     3361 aatgagatat caattgctaa aaaattagct ttatttttat acttaattaa tcctaaaact
     3421 tattttattt tatataaaaa agttctaaaa aaataaagtt tttttcgata tgttattttt
     3481 tatgtttatt aacttcggaa gactaaaatt gttactggta atgatacatt tgtaaaaata
     3541 aaaaagatga tttttaggaa gtgaagagag tgcataaaat tagtgttata gtacccgcat
     3601 ataacgtaga aaactattta gaaaactgct tagaatctat ttccaatcag acacattcaa
     3661 atattgaaat aatagtagtc gatgatggtt ccccggatcg aagtggggaa attgcagaag
     3721 aatataaaaa gaaagatatg cgattaaaag tgatacatca agtgaatggt ggtttaagtg
     3781 cggctaggaa ttctggtctt gattctgcaa caggagattt tgtgtgcttt attgacagcg
     3841 atgattggat agaaccagac tatcttgagt tactatattt tggtacgcaa acttatggag
     3901 cagatatctc ggtgctaaaa ataaaaaaga tttcaagttt agaacaacta aaagaacata
     3961 caattgctga gagaaaatgg gaaatatttg atagagaaca agcaatgaaa aacttattta
     4021 taaataataa aattggctat tctgcaaata ataaattatt caatagaaat ttatttgata
     4081 atataagatt cccactgggt aaattaatgg aagataaggc tactacttat ttgttgatcg
     4141 atcattccga aaaagttgct gtcaattcat ctgctaaata tcactattac caaagcccta
     4201 aaagtatact gagaggaagc ttcaatccta gaatttttga ttcttttgaa gtccatgaag
     4261 atatactagt attcatagat aagttttatc cggaattatc aattaatgtt agaactcgct
     4321 atgtctatga agcaataagg atgatgatga caatgattaa aaataactat accaataaag
     4381 aagattattt acgttctata aatattttac aacgctttga aaaagaagtg aaaattgatc
     4441 ccgacttttc accaaaatta aaaaaattag taaagttttt attaataaat aaaaacttgc
     4501 cttactatat gtcaagtagt aagatagcaa gtaaattttt gattaagata gaatctgtga
     4561 ggtaagagat gaagaatata aaaaaatttt tttcaaggaa tattttcccg gtttttatta
     4621 aaatattacc tattcaaaaa aatttaattg tattttcaag ttttggcggg cgtaattatt
     4681 ctggaaatcc aagagttatt tcagagaaaa ttcatgaaaa aaaattgaat tacagaatta
     4741 tttggttaat caaaaaagaa gcgattataa ctgatttacc agattatatt aaaagaattg
     4801 attccgattc ttttttagct gccttttatt tgtatacagc taagtattgg atagacgata
     4861 gtcggaaaac gattaagtat gcaaaaagga aaaaacaatt ttattttcaa acatggcacg
     4921 gtacgccttt aaaaaaaatt gaatttgatg caaaagataa gttacctaaa cgttacctaa
     4981 gctatgcgaa aaaagatagt gagtcaatta catatttatt atctggtaat cgctatagtt
     5041 ctgagaagta tgtttcagca ttcaatatcg ttccttctaa aatattagaa gtaggaactc
     5101 ctagaaacga tgaattagcc tctagtaaag agcaagtgtt tgacttgaaa aagaaacaaa
     5161 tcaatgtctt atttgctcca acttttcgaa ataatattga ggacaacgga attacgcaac
     5221 tagaatggtt aggtgtggat aacttacgtg aattttttaa aaagcaacaa caagaattaa
     5281 atttaatgac taaatttcat cctaatgttc atagtaaatt agcaactgat tctaaaagta
     5341 tagaatatat gaaaaaacat caaattactt taattaatga tgggattcct cttgaaatgc
     5401 tttttgaaga gatagatgtt ttaatcacag attattcatc tatttttttc gattttgcgc
     5461 taacaaaaaa accaattatt ctttttaact atgatgaaaa agaatatagc ttagaaagag
     5521 gtttttatat acagttaagt gagttaccaa ttataagcgt acaagatagt tatggccttg
     5581 ttgatgtttt taaaaaccaa caagaaaagc ttttgaaaag ttctcaagag ttacttgatt
     5641 atattggaaa ttatgaaaca ggggcaagta cagaaaaggt tcttgagttg ttagagaggg
     5701 atataaagtg aaaaaaacag taaaagatat attttataat gcgatatatc aagtcttttt
     5761 gattgttttg cccttactta ctatacctat acttagtcgt agaataggtt caactggttt
     5821 agggatttat gggtatgttt tctcaatatc acaattttta atgactgtca ttgctgtggg
     5881 aatgaatcct tttcgtattc gaaatatcgc taaatcaagg aaagataaaa aagcactatc
     5941 tttacaattc tggaatattt actttcttca atttttaata ggcttgagtg tttcctttct
     6001 ctatatagcc tatatttatt tttttgatgt aaagtataaa aatttatact taattcaatt
     6061 gatatttatt gcagggctaa cattagatat ttcttggttt tttcaaggaa ttgaagaatt
     6121 ttcgaaagtg gttattagaa atacagttat caaattatca tcggtattgt taataatcct
     6181 ttttgtttca actaaagatg atttgtggat ttatgtgttc attacttcaa ttactaactt
     6241 tttaggaagt ttagttttct ggttgagtat ccacggaaaa gtaggtaaac cactctttaa
     6301 ctctaatgtt tttaaacagt tgtggaaacc agcttttatt attttgactc ctcagttgtt
     6361 tatgcaagtg tacacaactt tagacaaaac gatagttggg tattttgtgg atccaacagc
     6421 attatcttat tatgatcagt ctcaaaaaat tgctcgtatt atattggcag tcttaacatc
     6481 aattactatt gttatgttgc ctaaaatatc taaagcacag gcagagggaa aatcagaaaa
     6541 tattttagtt tacacaaaaa aatcttttga ttacacgatt attattgcat taattctttt
     6601 tagtgtggtg tttatcaata caaaagaatt tgttccatgg ttttttgggg agaatttcaa
     6661 accgatgaca ataaacatgc ttatttcttg tctaattata gttgttagtc caattggtgg
     6721 agtttttgct aaccaatttg caattgctat agaaaaagat aaggagtatg cttatccaat
     6781 gatcattggt gcgattgtaa gtattattgg aaatgtagct tttgtgccta cttatggcgc
     6841 tgtagcggct acaatcgtac tcgtggtagt agaattatta gtctttttct ttagagtatt
     6901 ttttgtacga aaagaaataa aaatatccta tttctttggg aagaacgttg tcttattttt
     6961 tatagtcaat cttactttga ctacttcggc ctattacttt cttccacaat tattctcttc
     7021 ttcattttta gatatggcaa ttaaatcaat aatgattttc attatttatc ttccgtttgt
     7081 ttattttctt tttccagata tacgaaatga agtaagaaag attattcgaa aataaaataa
     7141 tgttgtaaaa aagtagttct ttgtcaaatc gggctgataa ggtcatttat gatcaacaaa
     7201 gttagtaaga tttactagct tttaatgcat agcttattaa cccgttgatt agaaaaatcc
     7261 acaagcgcat attggtaaaa ctgcaaaatc cataagcaaa tcgtttcaat gttttgatat
     7321 gtgtgcgaag attctctaat tttgtattaa aatacttttt cgaaaatgct agttaaaagc
     7381 ctttttcaga tatgaaaaag gctttttaag atagtgaagt ttcgagggaa cttctttggt
     7441 catttctcga a
//
LOCUS       DACECY010000100         7079 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.106, whole genome shotgun sequence.
ACCESSION   DACECY010000100 DACECY010000000
VERSION     DACECY010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.106"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(25..285)
                     /locus_tag="IWO38_002585"
     CDS             complement(25..285)
                     /locus_tag="IWO38_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314607.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(422..835)
                     /locus_tag="IWO38_002586"
     CDS             complement(422..835)
                     /locus_tag="IWO38_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ0314608.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1291..1776)
                     /locus_tag="IWO38_002587"
     CDS             complement(1291..1776)
                     /locus_tag="IWO38_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314609.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(1915..2130)
                     /locus_tag="IWO38_002588"
     CDS             complement(1915..2130)
                     /locus_tag="IWO38_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ0314610.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2354..3154
                     /locus_tag="IWO38_002589"
     CDS             2354..3154
                     /locus_tag="IWO38_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0314611.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3173..5041
                     /locus_tag="IWO38_002590"
     CDS             3173..5041
                     /locus_tag="IWO38_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0314612.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKVGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5034..5525
                     /locus_tag="IWO38_002591"
     CDS             5034..5525
                     /locus_tag="IWO38_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ0314613.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5513..6067
                     /gene="srlA"
                     /locus_tag="IWO38_002592"
     CDS             5513..6067
                     /gene="srlA"
                     /locus_tag="IWO38_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ0314614.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6085..>7079
                     /locus_tag="IWO38_002593"
     CDS             6085..>7079
                     /locus_tag="IWO38_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ0314615.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2483 a         1132 c         1371 g         2093 t
ORIGIN      
        1 ttccagccaa aactgactgg aagtttattt ttttctgaaa tattctaaca caaatatgaa
       61 aaagatggcc gaagctaata agccgatttc gtagcaaaac aatgagatac cagagtcgta
      121 ataataaaat cctctaatct tatggataaa atagatcatc ggacaagcag ataagagtaa
      181 gtatttgtta ggccttttga ataagtaaaa gaaagcactg gtcattatga taaatggtat
      241 ccaaaacaaa agtttactta agacaatcaa atgatcaact cccatatttt ttaccaagag
      301 agatcataac atctttatcg taaaaacaaa atattttttt gctcattttg aaaatttaaa
      361 catactcgtt cttatccaaa tatataaaaa tcgcccctca ttcatcgaga ggcgataagt
      421 tctaatttaa ctcaattttt tttgtttctt ttggctgata agctgtttta ggtaatacca
      481 atttcagcac accgttttta aaggttccag tgattttttc ttcatcaaca ttaggaagat
      541 agaatgaacg tttgtaagag accgaatgtc tttctttccg tacatagttg ccatcttctt
      601 tatcttctgt atgggactca tgattggcgg agatcgtcaa agtattatct gaatattcaa
      661 ctgtcgtgtc ttctttgtca cagccaggca tatcagcagt tagtttgtat tctttttcat
      721 tttcgactaa atctactttt ggataacttg aaacacctaa aaaatcattg aatgctggac
      781 taaacacgtc attgaaatca gggaacatgt cgcgaatgct aggtacattt gccatttttg
      841 atcatccttt caataagtat tttttattta tccctattta cgtaataact ctattttgaa
      901 aaaaatgcaa gtataatggt tagctttgtg aatatttcat ctaatacgat acaaaggcta
      961 tctttctaat ttgaaaaagg gatcattaaa ataataagaa gaaaatatgt caaaaggtaa
     1021 aaaagtaaat tcttctatcc aaatatagaa aaattgaatc tagaaaaccg cccgacaata
     1081 aatttcatta actcagttga ccgaaaatga agtagatacc aaaaatttat caagagagca
     1141 agtagagaag tgagtatatt cataagagaa atagctgtaa cgaattatta ggttaatgca
     1201 aatgcttatt tatactccgt ataactttct cagaaaaaat gataccaata atgacttgct
     1261 agtactgtaa atccgtataa tatcatctaa tcaaaatggt gttccatttg catctgtcat
     1321 cgtaatcgat ccttcgggtg acactcgata aaaaccaacg cttccagatc ctccttggga
     1381 acggatttgt ttgctggaag ctgtgaacaa ataatctcga tcaatttttt gataaaagct
     1441 aataacaata tcgtcacttt gtaattcgga acggtgggca ataagaaata gaagtgcttt
     1501 gtcagagttt tctaatggaa gagaaggtac agtgctttct tgcatttcag atgttgaaga
     1561 aatgctaaac gactgaatac ttgattggct atgttgcgaa tctacattac cagtagaagt
     1621 attagtaatg gaagtagtag aagtactttt agttgaacca acctcagttg ttgaaatggc
     1681 tgctgaagat tctttaacag aattctttgt tttatatcca caggcagaaa aaagtattac
     1741 agttcccaac aagcctgcgt aaatcccttt atgcatgata gctcctccta aaatagatta
     1801 tctttattca gtatatcact taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt
     1861 cccaataaag ggaaggttca tgaagataat aactttttta ataattatag ctttctaata
     1921 tctttcatca acttttctag taatcattgg gaaaaatttc aagcagtaga tcgcacaaat
     1981 acctacgacg atataaatga tcttcgcaaa aatagttgtt gaaccaccag caatcatagc
     2041 tactaaatca agttcgaaca agcctaccaa cagccagttc aaccctccca caatcaataa
     2101 agttaatgca atagcatcta aagttttcat tagaattctc ctttcttaaa tgataaaaca
     2161 attatatatc aaaaagagaa acattgctta tataacgctc aggaatttta gcaatctatg
     2221 caaaaagatg aaaatttttt ttgctcttcc attagtacac attttttagc tgaagctgta
     2281 aatttcatgt cttaattttt tttagtgagc ttttatactg gatttgtaag aaaaaagaag
     2341 gagaggatca catatgtcgg attggttgga tattgcagga aaagtagtaa ttgtaactgg
     2401 aggttcctca ggaattggaa gaagtatcgt cgaaaatttg ttgaagcaga atgcacaagt
     2461 agcgaatttt gatgtaactg aatgccgtat acagcatgaa aacctactat ctttaaaagt
     2521 cgatgtgtct tctaaaacgg acatagaaga agggatatat aaagtaatga aacatttcaa
     2581 gacgatcgat ggtctagtca ataatgcagg aataaatatt ccttctcttc taatagatag
     2641 aaatcatcca aaaagcaagt atgaattatc agaacaagtt tttgataaga tgatcgctgt
     2701 caatcaaaaa agtgtatatt tgatgagcca agcagttgga agaatcttgg tgcaaaaggg
     2761 atcaggagtg atcgtgaatc tttcttctga atcaggattg gaaggatcag aaggacagag
     2821 ctgttatgct gctacaaaag cagcgatgaa cagttttact cgttcttggg caaaagagtt
     2881 aggaaagagg aatgttcgtg tggtaggtgt tgcaccaggg atactggaag aaaccggttt
     2941 gagaacacaa gaatatgaag aagctctttc ttatacgaga ggtataagcg tcgaacagct
     3001 gagaaacggt tacagtcgaa caagtacgat ccctctagga aggagtggta agctgcaaga
     3061 agtggcggat ctagtctgct attatctttc agaaagatca agttatatca ctggagtcac
     3121 gactaatatt tcaggaggta aaacaagagg ataaaggata ggggggacgc acgtgacgct
     3181 agtaaacaga tggtaccaat tattgaaatt actagtcact cacaagaaag tttcaatcca
     3241 ggaagtgcaa aaagaaatca aaaccagtaa tcaaaccata aaaaaagata ttggggagtt
     3301 gaatgaagaa attgccggta ttgcaaaaat catccaaaaa aacaaacatt ttgaacttga
     3361 aatttatgat tttgattttt ttgatgagat catgcaagga aaattgaaaa cagccagtga
     3421 tttcaattcg gcatccaaaa gaatcgctta tttactaaat aggctgatcg aaacagagga
     3481 tttcttacgt atggatgatt tgtctgaaga agtcggtgtc agcagaggaa cagtggtcaa
     3541 ggatctaaag acattaaaag agatgatcaa tgattttgat gtaaagatta caggcacacc
     3601 aaataaagga ctaagaataa taggagatga gctagaatta cgactacttt tattttattt
     3661 tgtttctccc tacttttcag agacgaaaac agaacagttt ctagaagaaa aacaagcaat
     3721 caaacatttt cagaagaaga cgaatgctag taaacaagaa atcacccttt taacgaaagt
     3781 tatcggtatt tctttgaata gaatctctag taactatctg ttgaagaagc ccatcaatcg
     3841 ttacagtggg tgttttgaat atatccaaga attcaatgaa ctgatcaatc agttggaaga
     3901 aatctatgaa ctaactttga gccaatatga aaaagatttt atcagttttc ctttgaacat
     3961 catcaacaca agtataacga tgataaggaa agctaactct gatttgcgct tatatttcga
     4021 ccccatgatg atgcgtattc gacgattaga tctcattgat ctggatgagt ccttcttata
     4081 tcaagagatg aaacagcacc tctctttatt gataaatcgg gtaatttttc gttatcgatt
     4141 gactgattta ttttatggtg agatagaaaa aaagtatcct ttttcttacc aaatcgctag
     4201 cgaatgcata caagcattag aagctatctt gcaacataag atctctaaag tagagatcag
     4261 ctacctaacg ttgtatttgg agttagcatt tcgatatcaa aaagagcatt tttcctctaa
     4321 aaaagtagcc attgttcata cgacaggaaa aggaacagca ttaatgatcc aaagacaaat
     4381 caaaaggata ttggggaggg atatacaaat aacacatgtt tcagaagaac aatatgaaaa
     4441 agtagatcta aacaaatatt tcgctgtttt tacaacgatt ccgttaaaaa atgtaaatga
     4501 agagacaccg gtgatccatt tgactaatct ttttgatgac tcttggttaa gggcacaatg
     4561 gcaaaaagca aaaaaaaagc gattagccgg gtttcattct actgtttttt tctatcaaat
     4621 atggaaagaa aaaaagacgt atcaagaata tttaaacaaa ttagtcgctc ctttgatcag
     4681 aggctattat gtggatgaag aatttgaaaa aaggatcttg aaaagagaat atcagcaaag
     4741 cacatttgtt aacaaagaat ttgcttttcc tcacacaacc aaccagctat ctcaacacat
     4801 tgttttatca gtagggctaa taactgatca aggtttagca gaaacaggag gaaatggagt
     4861 tattttttta tttgccatac ctgaagaaat gacggctgaa gttgagaatg aattatttga
     4921 gctatacgat atcctatttg aactcataaa agtgggaata gatgagttta ttgaattagc
     4981 accgacacaa gaatcatttt taaaactaat caaaagtaaa ggagagagac ttcatgaatg
     5041 atatgtatct attcatagtt ttcgcagctt cagcttatgt gctgcaagca gttatcagtt
     5101 ggaagcaact aaagcatttc aatcaaacat atagcgaact gcgaagaaaa ggaaaagtag
     5161 cgattggtaa acgacctggg aaaatccgtt caggcacgat tgttatgcta gctatagacg
     5221 atcatgatca agttttagaa gtcagaaaaa tgcaaggggt tacatctttg gcaaaatttc
     5281 attcattacc tgtctatgcg ggtgaagata tccattacat cgatcgttac catccctcag
     5341 ttcgagcgga agatcgtttg acacaagcag ctttagaaaa tgccagagaa gtatacttac
     5401 gaatagaggc aggaaatgaa aaaagtgaac aaccttcatt gccttttatt gctatgctca
     5461 ataattttcg gatatggaaa agtacgatca aagaaaaaag gagtgacaaa cgatggatat
     5521 cataacaaaa tttgctgagg gatttatgag gctgttccaa acaggtgcag aaacattcat
     5581 aagctggatg acaggaatcg taccagttgt cttgatgctg atggttgcaa tgaatacgct
     5641 gattgctttt ttaggtgaag accgagtaac gaaagtagcc aaattatcag cgaaaaatcc
     5701 gcttactcgt taccttgtgt taccgttctt atctgctttt atgttgggaa atcctatgtc
     5761 atttacaatg gctcgtttcc ttccagaata ctacaaacca agctattatg ctgctcaagc
     5821 tcaattttgt catacgagca atggtgtatt cccccatatt aatcctggag aattgtttgt
     5881 atggatgggg attgctcaag ggatcgaaac cttagggcta aattcgatgg agctggctat
     5941 ccgatacttg cttgtgggac tgctgatgaa ctttattggt ggttggataa ctgattttac
     6001 tactggattt gtatgcagac agcaaggaat cgttcttagc aaaaaagtag agctttcagt
     6061 tgattagaga aaggagagtt ttaaatgtat ggtgatatta agatcgtaag aggaaacggc
     6121 gggtatggag gtcctttgat tattaggccg acaaaagata aacacaagtt catctatgtc
     6181 acaggtggag gtgagaggcc aatgatcgta gataaaatag aagaactgac aggtatggag
     6241 tctatcaatg ggttcaaaac atcaatacct gatagtgaaa tcgcgcttgc aatcgttgac
     6301 tgtggaggga ctcttagatg tggaatttat cctaaaaaag ggataccaac catcaatatc
     6361 gttcccacag gaaaaagtgg cccattagca caatatatca ctgaagatat ttatgtttca
     6421 gcagtagggt tagaacaaat acagccacta atggaaactg atgataacaa agaaacagta
     6481 aatactgcat ctccagataa aaagtacaaa tatgatactt ctaaaaagat aaccgagcaa
     6541 aaagcagagc agcaaaactg gatagcaaaa attggaatgg gcgcgggaaa agtagttgct
     6601 acattcaatc aatcagcaag agaagcagta gatacgatga tccggacagt gatccctttt
     6661 atggctttcg tttccctttt gattggtgtg atacaaggtt ctgggatcgg taatgtattt
     6721 gctaacatta tggcaccttt agcaggaaat gcagttggat taacaatcat tggctttatt
     6781 tgttcactgc catttttatc tcctttgctt ggtccagggg cagttattag ccaagtgatt
     6841 ggaacattga tcggtgtaga gattgggaaa ggaaatattc ctccccaatt agcattaccg
     6901 gcactatttg ctatcaatac tcaaaatgca tgtgatttta ttccagtcgg tctaggtttg
     6961 gaagaagcaa aagctgagac agtagaagta ggtgtaccat cagttcttta ttcaagattt
     7021 cttaacggca tcccacgagt tatcattgct tgggtagcaa gttttggatt atatagctg
//
LOCUS       DACECY010000101         7045 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.107, whole genome shotgun sequence.
ACCESSION   DACECY010000101 DACECY010000000
VERSION     DACECY010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.107"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            136..513
                     /locus_tag="IWO38_002594"
                     /pseudo
     CDS             136..513
                     /locus_tag="IWO38_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            513..1718
                     /locus_tag="IWO38_002595"
     CDS             513..1718
                     /locus_tag="IWO38_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0314616.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPFLNSSKK"
     gene            1734..2378
                     /locus_tag="IWO38_002596"
     CDS             1734..2378
                     /locus_tag="IWO38_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314617.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            2389..3195
                     /locus_tag="IWO38_002597"
     CDS             2389..3195
                     /locus_tag="IWO38_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314618.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTHQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFAVKLSHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKMKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            3217..3447
                     /locus_tag="IWO38_002598"
     CDS             3217..3447
                     /locus_tag="IWO38_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314619.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYLAAKKYFTADNK"
     gene            4005..4280
                     /locus_tag="IWO38_002599"
     CDS             4005..4280
                     /locus_tag="IWO38_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314620.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHELMDHRKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIE"
     gene            4322..4744
                     /locus_tag="IWO38_002600"
     CDS             4322..4744
                     /locus_tag="IWO38_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314621.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKPINESVHARVDPFDPTKFLKR"
     gene            4764..6356
                     /locus_tag="IWO38_002601"
     CDS             4764..6356
                     /locus_tag="IWO38_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314622.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKSLKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            6377..6712
                     /locus_tag="IWO38_002602"
     CDS             6377..6712
                     /locus_tag="IWO38_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314623.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYSATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            6858..>7045
                     /locus_tag="IWO38_002603"
     CDS             6858..>7045
                     /locus_tag="IWO38_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314624.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         2687 a         1078 c         1444 g         1836 t
ORIGIN      
        1 cttaataaat atttgtagga cgggaaaaca gaatgtcttt tatagacgag ggaacgccgt
       61 atgcggtgaa agtcgcacgt acggtgtgga ctgggggaaa agacggagat aatatcaaac
      121 tcttacctat cagtatcgat taatccagat tttattgaaa cggtaaatta tgtcacaaac
      181 tttcaacgag aaatgcgtaa agtagaagca atgactgttt tagctacaca atccccacaa
      241 gaaatggtgc cagaaaatat gtcttctgat aacttctcga aactgaagaa agtctttgaa
      301 ttttcttcta cgaagatttt catgcgtatg gatgaatcta ttttacgtca tatagaagga
      361 ttagtgggta aatcaatgac gaactcagaa ttagaatcca ttcctcaaca aaatcaagga
      421 gacgctgtga tcaattttgg ctcaaaagaa accattcgtg ttcattttag tccgaacaaa
      481 cgtcaattaa aattgtttga tggaggaaaa taatggaaaa ccaaaatgaa agtttaatca
      541 agcagtatgt taagcgtcgt gcaaagcgac gcttattttt gtggttgttt ggtacttctg
      601 caggtttgat tactattttg attactgtgt tcgtgaccct ttttctaata cttgcggcgg
      661 gttcgattga taattctgat tcagattcaa gtagtggtgg cgaagctttt acaggagaat
      721 attcggaagg attacccatt tataaagaaa tcaaaggaag aggccctttt tctgatgaga
      781 ttgcgcaata tgctgtaggt gcagcagtga aatataaatt attaccaagt gtgatccttt
      841 cgcaatacgg ttatgaatca gcgtttggta cgtcggcgtc cgcaagaaat gatctcaact
      901 attttgggat tacttggttt gatggttgtc tgtttcctaa aggaactgca cgaggaattg
      961 gtggcattga aggtggctgg tacatgaagt ttcccaactc aaaagctgca ttttcctatt
     1021 acggctttat ggtagccaca caatcaaatt ttaatgcatg tgtaggaaat aaaagccctg
     1081 gcgcaagctt attgatctta ggacgaggcg gttatgccgc tgcaggaatt acggaagata
     1141 gtccgtatta tacaggatgt atgtccatta tcacttctaa taaattaacc gaatatgatg
     1201 aattcgccat caaacattgg ggtgaaggtg gcaataacaa cggcacaatt actggtgaat
     1261 ggacgaatcc attccctggc agcagtttag ataaaagttc cttcagcggt ggtcaattat
     1321 ttggaacaaa tccaggcggt gaattccgtc cgaatggctt ccatgatgga ttagactttg
     1381 gttcagttga tcatccagga agtgaaattc acgcggtgca tggaggaaaa gtcgtgtacg
     1441 ttggcaatcc tggtatttca ggtttgggcg cgtgtgtgat cgtgattaat tacgacggtt
     1501 tgaatatggt atatcaagaa tttgcgaata gtacaggaaa ttctcgtgtg aaggttggcg
     1561 atcaagtgaa agttggtcaa gtaatcggga tacgagatac agcacacttg catttaggtt
     1621 tcactcgaat ggattggcgt caagcacaag gacacgcatt cacagatgat ggtacttgga
     1681 tcgatccatt accatttttg aatagcagca aaaaatagat caggagaaaa aatatggaaa
     1741 ataaaacttt aaaaatcgtc ttaacagctg caattagcgt tagtttaatc ggcaatattg
     1801 cgttgttttg gcagctgaaa gaggaacaag cagcaaatac ctataaaatt gaagaagtgg
     1861 ctaaaaacgc gtctgagaag ctcaaaaaaa attcctctga ggcacaggcg aagatcgaca
     1921 agttaaatca aaccatcaat gagaaaaacc aagaaatcga tcagctgaag caacaagatg
     1981 gcacaaaagc aaaagaggaa gtaactgaag cacaaagaaa gacggcagaa gagttcggtc
     2041 aattggccat tgataaatca ttaagcagaa atgaattaac ggaaaaacta aaagatgtcg
     2101 caacccaaga agtgatcgac aaattaagtc ctacagacga tgatcaccaa catgatgatt
     2161 acggaagtta ctcctttaca ttaggtgacg tgcaaactta cgctcaaaca agcagtgtaa
     2221 aagaggaaca aaattttgtc gtatttgttg attatacaat cggcaatccg caattcaaag
     2281 atgtcaaacc acaaaaaata aaaggtggat taactgtcac tgaaaaacaa gtagatggtc
     2341 aatggaaagt catagacttt agttatttca ctcgttagga ggaagaaaat gagtaagaaa
     2401 aaaggagaaa gacaacgttt caaaaaacga aaaagttatc ttcaatggct gggaatcttt
     2461 atgattctcg gctttttgat tcttgctagt ccgatttaca cgcatcagaa ggtagacata
     2521 ccagatgaag taagcacgaa cgcattttta aacacgaagg aactaacgat ggtttcaaaa
     2581 gaaaaaggag cgaaccagca attagttttt gaatttgcaa tcgacgatga agatcaaagc
     2641 ttattaaaag aactggctaa tcttaattat cgcgtagaag taaagacagc taaaggggat
     2701 tatcaagcaa taaaagccaa agtgattaag gtgtctgatg attattttgc agtgaagcta
     2761 tcgcatgtac cagaagaata tattgctatg cgattaacca ttattcctga aaaaattgat
     2821 ccaaaggtgg atatgcaaga accacaagat ctgatttatt acgttcacga agataaggtt
     2881 aaagatcaag tgaaagatga agattatgaa caacatgcga tcaattacaa aatgaaagga
     2941 tacaagaaag aacaaaaagc agctcaaaaa caaatggaat ctttccaagc aaccattgat
     3001 ttgaatgaag aattagtgaa gaagctaaaa gatcaattgc cttatcaagt cgcagatgat
     3061 caggaaaata ctgaaaacaa gattaatagc taccagcaag aaattgaaac atcgaaaacg
     3121 caaatgaaag atcaagaaga gatcattcaa aaacttcaag aaaaaattga tcgtatgaca
     3181 aaagaaaaga tttagaagga caggtggagt gaaaaaatga agcgaacaaa cttggctgac
     3241 aaagatactt tcgctgagca gaaagtattg attaaagcct atctcaaagt tgtaaaagct
     3301 aaagaaatat gtcgagaagt gaatttatct aatacaacaa tatctgagtt gaagaatggt
     3361 cgaaagaatt tagatacttt aaaattcaga tcatttgaag ctttgtattt agctgctaaa
     3421 aaatatttta cagctgataa caaataaaaa acaggagtat tttatgtgaa aaaagtatat
     3481 aaaaaatgct tatgaatcag ctatatcacc ttaaccattt gttaatgaga tgggagaaaa
     3541 cattaaccac gattctaacg caaacatcaa gcacataaaa catttaacac tcctcctttc
     3601 tggctgaaga tttcaaccaa acaggaagac agcgtatcaa ttatagcata cactttttct
     3661 taaattcatt tttcatatga caaaatattt ccttctaatc gacaaagtta gaaggatttt
     3721 ttattgtcta aatacacaaa taaccttaat ttataaagag cataaggaaa cgtgtaccca
     3781 ctttcaaaat aaagtgggta gataggctgt gcctatcacg tttctgaaaa aaataaatga
     3841 ccccaaaatt ttcaatttat gtgtaggttt atgggtagca aaaatatgaa tgtgggtagc
     3901 acaaaagttc atttgtgggt agtaaaattc aaatgtgtgt agcaatgtgt gtagctacac
     3961 acattgctac acacgctata acatatagtg aaaaggagat atttatggaa gtaaaaataa
     4021 gagatattga tgaaaaaacg attcaaagaa ttgaccgaag atgtattgaa ttaagtgaac
     4081 gcaccgggac taaatggagc cgaaatcgat acctaaaagt tttgattgaa aatgattttg
     4141 accatgaact tatggatcat agaaaagatc aatttgatcg gttgttagaa aaatttgtgg
     4201 atattcaaac ttataatacg aaaattttag aagaatatat tgcaacaaat aatcaattaa
     4261 tcgccttgtt aattgaataa taaaaatagt attgtgaatt tataaaagga ggaaatcaat
     4321 tttggcaaaa gcaacaagaa aacatgtata tctttacgat atgcatatcg aagtacttga
     4381 tgaaataatt tctactcatg aagatatatc taattatagt gaagcagtca gacatttgtg
     4441 tatgatgtat gatccaaaaa tcgaagaaaa aataaatcca attcaaatta aattgaatgc
     4501 aatgggaaaa gaaatttcta tattaaccga aatggtttca gagtttgcgg atcttcattt
     4561 gaaagataca ggaatactaa ttggtacaaa ttcaactgtc tatcaagaag caaaagagcg
     4621 tgtggaaggg aaaataaaag cgaatcaaac gaaaaagtat tctcctaaga tcaagaaacc
     4681 aataaatgaa tctgttcatg ccagagtaga cccgtttgat ccaacaaagt ttttaaaaag
     4741 ataaaaagta gaaggggaac atgatgaata aatcgcctgc agtcattctc acttgtcaat
     4801 ttaccttgcc caataaaaaa tctttttcaa catacattga ttatatgaca agagaaaaag
     4861 cgctggaaga aaaggaacga cgcacgccgg acgaagaaca ggaactgcaa cgaatcaaga
     4921 atgctttaga ggatttttat attcctgaag gagaaacttt ttcggagagt aagacagata
     4981 aagaattcag tgaaacgtcg aatgaagctc aatcgttgat ggaacatgga atcaattttg
     5041 acaagttaga ggaacaagat tttaccaaat atctttctta tatgacccgt cactatgcgt
     5101 tagaacaaaa gaaagaactg acgcaaaggg aacaaaaaga ataccaacgg ttgaaccaag
     5161 caatgaaaaa atatcaaaca gagccacata aaaaagcttc aggaaatctt cctggagttt
     5221 tttctatggc ttcagacgaa gtgaaaggaa gtgatctgaa ggagatcaaa caaatcttcc
     5281 aaagagggca acaaaaaggc tcaatcattt atcaagatgt tgtctctcac gacaattcgt
     5341 atcttgaaaa aatgggcttg tacgatccca aaacagatac cttagatgaa gaaggattaa
     5401 aagcagctgg aagaaaaatg atggagacat tattcgagaa agaacaattg aatgaaacag
     5461 gttactggat ggcaacgatt caccgtaaca ctaagcatat ccatattcat tttgcagttg
     5521 ttgaaaaaga aaatacgcgt aaaccttaca cggaggttga aaatggtgtg agttatatcg
     5581 taccgaaagg taagagaaga caagcaacat tagacgcaat gaagaccaat tatgtacatg
     5641 agttagaacg tttttcttat gaaaaggatc gaatgattgg tatggagaaa cgaaatttat
     5701 tgacgcggaa aagtgatttg cgtaacacct taacaaaagt ggtaaaagag ccagcgcgtt
     5761 acgatcgaca cgcaatgaac ttattaaatg aagtatataa aaccctacct gacaaacgtt
     5821 cggaatggca ttatggggat gaaaaacgaa ccaaactttc accatacaca agagagaaac
     5881 tcgatgagtt aaccaaatat gtgttaacaa aagaacctga atatcaagaa tatatacaga
     5941 tttcaaaatc attgaaagaa gaagcaaaaa gtctttatgg ggaaagcaag agagaatcaa
     6001 aggatgcaga aaaaaatgct ctctttgatt tgaagaagcg cacaggaaat gccattctaa
     6061 cagaactgaa gaaacaggat agtcaaataa aacctttaat agaaagtatg gatcagatgc
     6121 aatctattta caatgaacga tcctttcgaa cgcttaaaca gttaccaacg aatgaataca
     6181 aatatagtta cgaagaattc atgaagcgca ggaaacaatt ccaacaaaga gttgtcacgc
     6241 aacggagttt gcgtcaatta aatcgaacga ttaatgccca tgaagaaaaa taccacgcaa
     6301 gtaaagccta cgaagagcaa caacgaagaa ttcaaatgga acaagaaaga cagtaaagac
     6361 agtaggagga atagcaatga aacaatattt agatcaatgg aaagttatag aaggatcatt
     6421 aagagaagaa cgaatcgaac aacttcccga ttgtttagaa aaagagcatt tgtttcagat
     6481 aagagaaatg ttgagaaacg aacagtttga tccaaatcaa tttttagtcg ttgagtactc
     6541 agctactggt gtgtactgtt gtaatcatgt gaagggtgaa aaatatttta tcattcaaga
     6601 atatgagggc aagttagcgc cttattatac tacttgggaa atgaatgaag aggggatcaa
     6661 taacttcccg tgtaaaagca ttgaagagtc tatttcactc acagaatgtt agttgttaag
     6721 tgaataaaag aagtaaaaga gtcctgaacg taattgtcca ggactctttt tttcaatgaa
     6781 ttgttttccg ccaattcaat gagtggtgcg atttgcgact acattatatc aaaagaaaga
     6841 gaagggagga agaagaaatg gcttattcaa cgattggagt tgttgttgaa aaaagcagag
     6901 acaatctcgt ttttgtgaca gaaattcaaa cgggcagagc ttttgtcgtc acagacaaag
     6961 cggcaaaagc gtatcaaagt ggcgatattt taacgttaaa tctgactacg aagacgttcg
     7021 tcgatgcggc agaagattat ccatt
//
LOCUS       DACECY010000102         6531 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.108, whole genome shotgun sequence.
ACCESSION   DACECY010000102 DACECY010000000
VERSION     DACECY010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.108"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(142..1086)
                     /locus_tag="IWO38_002604"
     CDS             complement(142..1086)
                     /locus_tag="IWO38_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ0314625.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDMD
                     KQVTFIEGDVSDSQLMERIMKEYQFAYIFHLAAVASVADSVERPVETHRVNFDSALLL
                     LELARKYQSNLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKAEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILSGMERYLKTEI"
     gene            complement(1220..2611)
                     /locus_tag="IWO38_002605"
     CDS             complement(1220..2611)
                     /locus_tag="IWO38_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HAQ0314626.1"
                     /translation="MLNERFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFVINIFIQFS
                     FIALSGSRTTLVCLLITTFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAHAWAHVPHITEVKKDTSDSSGTTSSNKVDLKRPDTDSGDVSNLRFELWKSAIEI
                     FKSKPISGGSPGYYIEYAHNKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKALRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            2737..3147
                     /gene="tagD"
                     /locus_tag="IWO38_002606"
     CDS             2737..3147
                     /gene="tagD"
                     /locus_tag="IWO38_002606"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HAQ0314627.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGHFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            3323..3736
                     /locus_tag="IWO38_002607"
     CDS             3323..3736
                     /locus_tag="IWO38_002607"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ0314628.1"
                     /translation="MISISHSIKELFTIKDKNIEINSFQETFYKDKKAIVIEGPLVRT
                     FRHCLFCKESAKQIEKNGKKISMIILNRSGNKQTYLRLRKQHYHCQSCGKYFTAHTYL
                     VAPSCFISKQVHYTILEELTERQALKTIAKRCDFQ"
     gene            3893..4150
                     /locus_tag="IWO38_002608"
     CDS             3893..4150
                     /locus_tag="IWO38_002608"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0314629.1"
                     /translation="MTFCPAGEKKDLIAYFMRFTRKAHMGVRYIVTDMNAPYFSLVKE
                     PFPNAQIIIDCFHIGQHLNKFFDRVRKRVMKQLNQKCQRHL"
     gene            4237..5475
                     /locus_tag="IWO38_002609"
     CDS             4237..5475
                     /locus_tag="IWO38_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAQ0314630.1"
                     /translation="MRKDIREGVMIYVINEIKPNYAALAKQYDCDYRTVKHAYEEAQV
                     KESKPPERKKRPSKLDPYREIIQDKIKDQCSAYSIFKFIEHKGFGGSYSLVKTYCRQI
                     KQAKVHKATVRVEHSPGLSAQVDWKEDMRLISNQGEIFEFNIFLYVLPYSKKKYVTLT
                     FDRKQDTLFECLDDAFYQTGGVPKEIWFDNMKTVVDHSRSQFSKPHFNKRFYAFSKDA
                     GFTPIACRPFRPQTKGCVEALARTVDRLRVYNHEFYDAVELCHLVNNLCVELNNEVSQ
                     STDQTPDERWQTNEKEHLHPYQKNLLNPYFEDFIRRKVTKEEMVIFRKCRYSVAPRYI
                     GKEVEIELSDKEDQVQIYYNGELIRSHPLTTKQLNYHEEDYFQILKSDVMSHKEDDGI
                     RAYTRESLKQYDLVEVADEE"
     gene            5465..6205
                     /locus_tag="IWO38_002610"
     CDS             5465..6205
                     /locus_tag="IWO38_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0314631.1"
                     /translation="MKNSHYQELMTNLETLGLNHMREYVPNYIEVANREELSLTEALL
                     ELTRREVDHQNKQKMNRVVARARFSKRTSLEEFDFAFQPSINKREVLDLRHMGFVEKH
                     ENLVFIGSPGVGKTHLTIALGIEACRQGYRTLFITCHELLLRLRTAYEKGTLERVLKR
                     YARYDVLIIDEIGYLPIQKQEADLFFQLLAMRYEHRSTIITTNIVLSRWGELFQSSEI
                     AAAILDRLVHHVKVFKITGKSYRMRDKI"
BASE COUNT         2220 a         1225 c         1100 g         1986 t
ORIGIN      
        1 aagttctctt tttcatgtaa aatatttaat tatatatttc ctaaataaaa agtaaaacaa
       61 gaattaaata gttggttata caactttaat tcttgtttta ctttttagaa ttatttatcc
      121 aaagatgcaa tcattttttc atcaaatttc tgtctttaaa taccgttcca ttccgctcaa
      181 tatatcaaat ttaggctgat agcctaaaga acgaattttt gaaatatccg ctaaggaatc
      241 acgaatatcc cctgaacgct ccgcttcata tttaagcgct agctctgttc ctaagatttg
      301 atcaattgaa tggatcaatt caagtagcgt agtgcttttt cctgtaccga cattgaattg
      361 ctgtcctaaa gctttttctt ccttggcaac aagtaaaagt gcttgtataa catcttctat
      421 ataaacaaag tctctagact gactcccatc gccaaataaa gtaaattccg ctttttctcc
      481 tgccaattgt tttttgtaac gatccactag aatcgaaatg actccgctat atggagagtt
      541 tggattttga tttggaccat aaacgttaaa aaatcggaca gcactagtcg gaacatcata
      601 taaatgacag taatccaaaa catattgttc tgctgcaaat ttatcgatag catatggtgt
      661 taatggacgg atgactgatt cttctttttt tggcagtgtt ggttcatcac cataaactgc
      721 agctgatgaa ctaaatacta atcgttttaa attagattga tatttccgtg ctaactcaag
      781 caataaaagc gcactgtcaa aatttacacg atgtgtttct acaggccgtt ctacagaatc
      841 tgccacacta gctacagcag ctagatgaaa aatataggca aactgatatt ctttcatgat
      901 tctttccatc aattgagaat ctgatacatc cccctcaatg aaagtcactt gtttatccat
      961 atccaagttt tctttttttc ccattgataa atcgtcaact actacgattt tgttttcttt
     1021 tcccaaataa ttcgctagcg ttgaaccgat aaaaccagct ccgccagtaa tcaatatagt
     1081 gtccatcaaa ttcactccaa ttgcacattt tcgtatcaaa taatattagt ttaccatttt
     1141 tatattgtaa aaactataaa atttgttgaa gttgggataa ctagttttct tttctacttt
     1201 catataccta aaaatagttt caattagtta ctccttcttc tttagatata gttttttgga
     1261 tagaacccag atatccccaa aaaataaatg aacctatcgt actaaccaaa atgagttctg
     1321 tgataaataa tgccgaaact gctattgtaa gaatcattaa aatagcaaaa aagtgcttat
     1381 cttttactag atttggttgt gtgaaacaat aacgcaaggc tttaaagcat tggataaata
     1441 agaaggttaa caatgtcaat agacctaatc ctcctgtagc agccatcact aagaaaataa
     1501 aattatgagc agttaaagta tcttttccca ttaatgtata tggtagttta ttatgtgcat
     1561 attcaatata gtagcctgga gaacctccgg atatcggctt actcttgaat atttcaattg
     1621 cacttttcca aagttcaaac cttaaattag aaacatcccc gctatcagtg tcagggcgtt
     1681 ttaaatcaac tttattgctg ctagttgttc ctgatgaatc agatgtatct ttttttactt
     1741 ctgtaatatg cggaacatgt gcccatgcat gagcagttat atctgttaca taatacatcg
     1801 caattatact tagcaatcct actactaatg cagttagcca tctttttaaa gtattagttt
     1861 taagaaaacg actactataa cttgtaaaga aactaaaaac aaaagttgtt attaataaac
     1921 aaactaatgt ggtgcgtgaa cccgataaag caataaacga gaattgaata aaaatattaa
     1981 ttacaaaaaa tattttaaat agaagattta aatgcttgta agtgaccaaa taatatatag
     2041 caataatgat gacgattaat gatataaccg caccaaaatt tggatctgta tatacaccaa
     2101 ataaacggtt gcctaaccac cctaaacgaa ctgggtaata cttatatttt attggaattt
     2161 ttatgccata ttgaatgaca aaccagtata atgatactaa attagctaaa aaccatacca
     2221 atgaaagtat ccaggtaaat actttgaaaa aaaagttact actatagtct ttgccaaact
     2281 gataaatcat aaagaaaaag attaattgcc acatgattaa cttaatattt tcgctatatc
     2341 catattttaa attcattaaa ctgcttacta gcatcgcgcc aataaaagca attaatagcc
     2401 aatcatattt ttttagattt ttttctctaa atgcaataat tgcatctata atgatcaaga
     2461 caactcccgc tattccagca ggccaaaaaa gtaaagtatc atatctagta ggtaatagta
     2521 aatacgggcc tattgctcgt aacattaaaa taaaaccaaa ataaataaaa taccaacctt
     2581 tcacatattt taaaaatctt tcattcaaca tttttcctcc atccacctat ttctcataaa
     2641 tatttcttat cctttttagg ataaactggt cctttcaatt ctaacaaaaa aatgctaaac
     2701 taacaatgta tattaatgaa tttcgagagg tgaaagatga aaagagtaat tacttacggt
     2761 acctttgatt tgcttcatta cgggcatatc aatttactga aacgagcaaa acaatacgga
     2821 gactatctta ttgttgccct ttctacagat gaattcaatt ggaatgcgaa acaaaaaaaa
     2881 tgttatttta gttatgaaaa aagaaaacag ttacttgaag ctattcgcta cgttgattta
     2941 gttattcctg aagaaaattg ggaacaaaaa gtaaaagata taaaagaata ccatgcagat
     3001 tatttcgtga tgggcgatga ttgggctgga cattttgatg aacttgaaca actaacagat
     3061 gcacaagtga tttatttacc aagaacacct gagatctcta caactcaaat aaagaacgaa
     3121 cttaattctt taaatagtga taaataatat tttttaaaca ataaaagaac aatatatctg
     3181 gataagacgt aaaaaaatgg ctctataatt tctaggactg atagaatttt ctgtcggtcc
     3241 tagatttttt gtaaaaaaaa acagacataa aaaaattccg atagaataga gtcgtctaaa
     3301 acatcaatct gaaaggaagt ttttgatatc catttctcat tctatcaaag agctcttcac
     3361 tattaaagat aaaaatattg aaattaattc ttttcaagaa actttttata aggacaagaa
     3421 agccatcgta atcgaaggtc cattagttcg cacttttaga cattgtctat tttgtaaaga
     3481 atctgcaaag cagatcgaga aaaacggcaa gaagatttca atgattatct taaatcgcag
     3541 tgggaataaa caaacctatt tgcgtcttag aaaacaacat taccactgcc aatcatgcgg
     3601 gaaatatttt actgctcata cgtatcttgt cgctccttca tgttttattt cgaaacaagt
     3661 tcattacacg atattagaag agttgaccga gcgtcaagca ttaaagacaa ttgcgaaacg
     3721 ttgtgatttt cagtaacgac agttcaacgg acattatctg ctctatcggc aacgctgcga
     3781 cagaagctag actatttacc tcattgttta ctattcgacg agtttcgctc tctttcaatt
     3841 ttccatggga agttctcctt ttcttgtatg aattgagaga cagggaagct ctttgacatt
     3901 ttgtccagcc ggagaaaaaa aagatttgat tgcttatttc atgcgtttta caagaaaagc
     3961 tcatatgggg gttcgataca ttgtcacaga tatgaatgcc ccttattttt cattagtcaa
     4021 agaacccttc ccaaatgcac aaatcatcat tgattgcttt cacattgggc aacacttgaa
     4081 caagtttttc gatcgtgttc gaaaacgtgt gatgaagcag ttgaatcaaa aatgtcaacg
     4141 gcacctttaa aatgtaggaa aacggcaatt taaaattgtt ggtttaccta catttttgta
     4201 tgctcttctt atcccaaatg aaatggaggc tcagggatga gaaaagacat acgcgaagga
     4261 gtgatgatct acgtgatcaa tgaaatcaaa cccaattatg ctgcactagc aaaacaatac
     4321 gactgcgact accgaacagt taagcatgct tacgaagaag cccaagttaa agaatcaaaa
     4381 cctccagaac gtaagaagcg tccaagtaag ttagatccct acagagaaat catccaagac
     4441 aaaatcaaag accaatgcag tgcttacagc attttcaagt ttattgagca caaaggattt
     4501 ggaggaagct acagtttagt aaagacctat tgtcgccaga ttaagcaggc gaaagtacac
     4561 aaggcaaccg tccgagtcga acatagccct gggctttctg ctcaggttga ctggaaggaa
     4621 gatatgcgac taatcagtaa tcagggcgaa atttttgagt tcaatatctt tttgtacgtc
     4681 ttgccatatt ccaagaaaaa atatgtcaca ctaactttcg accgaaaaca agacacactc
     4741 tttgagtgct tagatgatgc gttttatcaa accggtggcg ttcccaaaga aatctggttc
     4801 gataatatga agacagtcgt tgaccattct cgtagtcagt tttccaaacc gcatttcaac
     4861 aaacgttttt atgctttcag taaggatgcc ggcttcacac caattgcctg tcggccattt
     4921 cgtccgcaaa caaagggctg tgtcgaagct ttagccagaa ctgttgaccg cctacgggtc
     4981 tataatcatg aattttatga tgccgttgag ctgtgtcatt tagtgaataa cctttgtgtt
     5041 gagttaaata acgaggtttc tcaatcaact gatcaaacac cagatgaacg ttggcagacg
     5101 aacgaaaaag agcacctgca tccataccaa aaaaaccttt tgaatcccta ttttgaagac
     5161 tttattcgcc gtaaagtcac caaggaagaa atggtcattt tccgtaaatg caggtactct
     5221 gtggcgcctc gctatatcgg taaagaagtc gaaatcgaac tctccgataa agaagaccaa
     5281 gtacaaatct attataacgg agaactgatt cggtcgcatc cattaacgac aaaacagctg
     5341 aactaccacg aggaagacta tttccaaatc ttgaaatccg atgtcatgag tcacaaagaa
     5401 gacgatggaa tcagagcgta tactcgtgaa agtctcaagc aatatgatct ggtggaggtt
     5461 gccgatgaag aatagccatt accaagaact gatgaccaac ttagagactc ttggtttgaa
     5521 tcatatgcgg gaatatgttc ctaactacat cgaagtggcc aatcgagagg agctttcttt
     5581 aacggaagct cttctcgagc tgacacgcag agaagttgat catcaaaata agcaaaaaat
     5641 gaatcgtgtg gtggcacgcg cccgcttttc caagagaacc agcctggaag agtttgattt
     5701 tgccttccag ccaagtatca ataagcgcga agtgctagac ttaaggcaca tgggctttgt
     5761 ggaaaaacat gagaacttgg tttttatcgg cagtcctggt gtaggcaaga cacatctgac
     5821 gattgcattg ggaatcgaag cctgtcgtca aggctatcgg acactattca tcacctgtca
     5881 tgagctgctt ttgcgtctgc gaacagccta tgaaaaaggt accctagagc gtgttctcaa
     5941 acgatacgct cgctatgacg tcttgattat tgatgagatt ggctatcttc cgattcaaaa
     6001 acaggaagct gatttattct ttcagcttct tgccatgcgc tatgaacacc gctctacgat
     6061 cattacgacg aacattgtct tatctcgttg gggggagttg ttccaaagtt ctgagatcgc
     6121 agctgccatc cttgaccgtt tagtacatca cgtcaaggtg ttcaagatca caggcaagtc
     6181 ttatcgtatg agagacaaga tctaaagtgt cgattgtcga caattactat tgccgaaaac
     6241 ctacattttt atcctgccca aaacctacaa ttttaagctg cccttgacat caaaaagaca
     6301 cttcacaagc taagtgctat cgacaattga aacctttgta taaattactt ctataatcag
     6361 atgacgagtt ggattataca acattcaaga aatggcagaa ttttcaatgg cgctatttaa
     6421 cagaaagtga agtagttgat tgtctaccct caatctctta tgaacttaaa atggcttagc
     6481 agtattatca aaagctgctt aacaattatc acaagaaaca gctagctact t
//
LOCUS       DACECY010000103         6509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.109, whole genome shotgun sequence.
ACCESSION   DACECY010000103 DACECY010000000
VERSION     DACECY010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.109"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(122..1288)
                     /locus_tag="IWO38_002611"
     CDS             complement(122..1288)
                     /locus_tag="IWO38_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0314632.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(1343..1993)
                     /locus_tag="IWO38_002612"
     CDS             complement(1343..1993)
                     /locus_tag="IWO38_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0314633.1"
                     /translation="MSIKILLNLWFSCYNSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            2068..2268
                     /locus_tag="IWO38_002613"
     CDS             2068..2268
                     /locus_tag="IWO38_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0314634.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            2280..2606
                     /locus_tag="IWO38_002614"
     CDS             2280..2606
                     /locus_tag="IWO38_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314635.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            2759..3811
                     /locus_tag="IWO38_002615"
     CDS             2759..3811
                     /locus_tag="IWO38_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ0314636.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGT
                     NQYSVDNSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLS
                     TKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAE
                     KEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNK
                     ALELIELQISEGIDKQ"
     gene            3887..4105
                     /locus_tag="IWO38_002616"
     CDS             3887..4105
                     /locus_tag="IWO38_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314637.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            4092..>6509
                     /locus_tag="IWO38_002617"
     CDS             4092..>6509
                     /locus_tag="IWO38_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314638.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSETVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVREN"
BASE COUNT         2343 a          910 c         1187 g         2069 t
ORIGIN      
        1 gcacgtaaag cttcacagtt ttcaaaacgt taatcgttgt tataccaacg atttcagaca
       61 ctttctcatt gcgagaaggt gtcttttttt cgttctggta atgagaaagg taatgacaat
      121 attataaacc agtgtaagca atgaattttt ctaacgcatt tcgttgagct ttttcagtaa
      181 catgagaata agtgttaagg gtagtttcag catttttatg acccaatcga tcttgtgttt
      241 ctttgatact tgctccacct tctagtagaa gagtagcgtg agtgtgccgg aatccatgaa
      301 tagtaacgtg cttaaattta tatttcttct ctaaacgttt caatatatta tttgaatagt
      361 ccgcatgcaa aggttgatta atgtcaccgt ctgcatttac ataggtaaag ataagttgat
      421 tttttgtatt tgtatttatg ccaaactgca aaagctctcg agcttgttct tttttccaga
      481 tttttaacat ttcaatagtt tgtgcatcaa ttggtaatat tcgttcggaa gatatatttt
      541 ttgtattttt ttgaatcctg acagtttttc ttcttgcatt cttctcttca tttcgaatag
      601 ttgcatagct ctttctaaca tacattagct gttcttcaaa ataaatgtca gaccaatgaa
      661 gagcagctag ctctccttta cgtataccag tagtaatcaa aagctggaag attaaaatat
      721 gttttaaagg ctcttcttta ttgagaatat cccaaaaagc ttttagttct tctaatgaaa
      781 agtaattttc attttctttc ttttcccgct ttggtagatt tgtatttgcc ataggatttc
      841 ggtgtaatag ctctgcaaaa atggcatact caaatatttg agagctgtaa gacttaagct
      901 gtttgatatt cttgaatttt aaagcccatt cattgactgc attctgacag tgaacaggag
      961 taatcttaga aatggactta ttcccgaatt ttggtagtat atgcaagcgg aaaaggtctt
     1021 ttgtacgttg atatgtcgtt tcttgtacac gattcctata agaaggcaac cattcttcat
     1081 acactgttat aaacaagtca ttagaaggct tatgatagcc ggtatcttca atctcgacct
     1141 tttttcttgc ttcgtgtaac ttggcttctt tttcagtttt aaaccctttt tttacatagt
     1201 attcttgctt tccattttca tctttacgaa catacaagcg gacactatat cgtgcaccgc
     1261 tttttgttgt gtacttttgt attgacataa ctaatctctc caatctgata acttggcgag
     1321 ttggctgatt gaataattag ttttattgaa gttttgatat taatcgagct gttatctctc
     1381 tttttagatt gttaacctgt acttgggtta agttgccaaa taactcagat tctaactctt
     1441 ttctttcatc gatatttaag tttaaagctt tattaataaa cttcttttgt ttggcaaata
     1501 gaatcgtgta ttcaaaggag tcctctagaa tactttttaa aaaaagaaag tcggagtcat
     1561 cttcttgaag tttgacttca agtaaagaag tcatggaaaa gtatagatta gataattcag
     1621 taaaataatc tgagtcaaga ctggcaatat tcttgactat tttgtccttt ttttctaaac
     1681 gagcagtttt tattctatca agattaggtt gttctatgga atttgtttcg tctaatccca
     1741 tcaaataact tacaggaaca tggaaataat cagcaaggat attccatgtt tcagtatttc
     1801 taggatttcg ctcaccacgg taatagttcg taagagttgt atagttaata ccagtttctg
     1861 tactaatttg tttaatggtc tttttactgg aatcaatata aaattttatt ctattcaaag
     1921 taacgccccc tatagtaaat tccaaactat tatagcatga aaaccacaaa ttgagtaata
     1981 ttttaattga tattactcgt tttgtggttt aaaatgtgct tattaagata aaaaccacaa
     2041 agtgaataat ttgaaaggag gcgctgaatg ataactctta atgatcaatt cattagaagc
     2101 ttaagaagac atagagccga tcttatactt actaaaaacg atgcagcaaa attgataggt
     2161 ataaatcgga aaacatatgt caaaatcgaa aatgggagca aagaaagtat tagagcatct
     2221 acttatcaaa agttggtaaa ttggttactt aatgatttga aaatctaaat aaatacaact
     2281 tggcgagttg gctgattgaa gaaatagaaa atgagaggag aaaaataatg gatgcaggaa
     2341 ttacagtaat gttagataaa caacaaacga atcaattgaa aaattatgtt tttgaaatga
     2401 caaaagaggc tattgatcaa gcaagaattg ataccggatt agaaagacca tttctaaaag
     2461 gaaaagagat ggctaaatat ttgaatgtat catatacaac ttttttgaaa tttaaaagaa
     2521 tgggattgcc agttatttta ttagaaaaaa tggagttgtt ttcaaaagaa gaatgcaaaa
     2581 aatggatatt gagtcatcaa atttaaaaaa cacctcgttt tttaaatttg atgaagtaga
     2641 aaaaattaaa aagagtgttc ccgtcagttt gcagatcggg ggaacactca ataataaagt
     2701 tctgagaaaa ctttatttga atctattata gcaaataaaa tcagaagcag gtgtgacaat
     2761 gaatgaaaaa gaagttgaaa gcttagttga aaagcaaggc tattctaatt ctgatatata
     2821 tgcagaatta tactatcaat atcctatttt tttaaacgta aatgaaaaat ataagaaaat
     2881 gaaactaagt tctaagttag tttatatgtt actaaaagat agaaatagac tttcattgaa
     2941 aaataacttg tatgatgaga atcaaaaaat ttttttgata tttgaaatca aaaatctcgt
     3001 agaaatttcg ggaatgtcaa aaaatacaat cttatcagca ctagatgaac ttcaaaaagt
     3061 taaacttctt gtgaaaagaa aaaatggctt caataaaagt aaaatgcaaa tgttcccaaa
     3121 tttttattat ttgttaaagc ctgttctgtg caaagatgat gtttataaaa ttaataagat
     3181 aacccatcaa gaaaatagac aaaaaaactt gatagttcaa aatttgaact atggaacaaa
     3241 tcaatattct gttgataatt caaaatttga actatgcgac acatcaatga aaacaagaga
     3301 caaaaacaat tttgctgata gttcaaaaaa tgaactagaa ttatataagt cttataaaga
     3361 tagtaaagat attaaagaat cacataatga tgatcttctt gaatcacttt caactaaaaa
     3421 taataaatta gatgaagaat tgatcgaaac atttattgaa gaaaatcttc ttaaacaaat
     3481 atggggagaa agtattgtga attgcttaaa actggctagc aatagtgatt atcgtcaatt
     3541 cgacaactgg tacaaaaaat tcaaatatgc gattagatct gctgaaaaag aacaaaaaat
     3601 tcaactgaaa attatctatg aaatttgtaa taacaagtat tttgtagatc atgttagaga
     3661 acagctatca atgactttac gtgacttgat acgaagagca aaaacagatc atcgtatcaa
     3721 acaaaaagac gattatatct ttgcttctct aaaaaataaa gcgttagaac taattgaatt
     3781 gcaaatcagc gaaggaatag ataagcaata agaatagaat tgagtcaaat gaaatggagg
     3841 ggtatctcca cttcatttga gaactttgaa tataggaggg gttattttga ttaaaaatcg
     3901 tttaatagat caagtttggt gtctagatca aaatacagat agtctcgaat taatcgagac
     3961 tatttgcttc aacactatag ttctcgattt aagagctgaa aatgataatt gtgtaactca
     4021 tgtcataatt aatcaaaaaa tggctcagaa gctctctgag agcctcagaa aatgggctaa
     4081 gggaggtaac gatgaaaata attgaaaaaa agattgccaa taaaaaaata aattggttgg
     4141 ttgttaattt tcttgttctg atagctatga ttatttttac aagtaatatt gctcatgcga
     4201 gtattttcaa ttggtttgat gacacaaaaa gtcaaacaga gtttttgaat aattctcttt
     4261 atagacctta cctaaaaaag tacaatggag atttccttag tcagttcggg atttggctcg
     4321 gatgggctgt ggtaaaaggc atgtttactg tgacagattc gattcaaaat atgattccag
     4381 atgtgctgga tttgttcaat ttcattgaaa gtacagggct aaataatgtt tatcaatcag
     4441 ccgtaaatac aattgtcatt ggattaatga tcctttcatt gatgtttgtt ggttacaaaa
     4501 tgattactgg aaaaggaaca gtggatttaa aatcggttgg aatgaacgta gtgatgtcta
     4561 tagcattaat acttttaatg ccaaccatga tttctagtgg tattcaattt gctaaaacat
     4621 tttataagga ctctactacg attacaaatt cagatgacgg agtcgcttgg tcattgataa
     4681 aacaaggagt aacagattta gcatatatta ataaaacaga tcagtactca agcattgata
     4741 aaacggagga tcgaaataat ttaacacgaa aaaattttca acagacggat ctcacacaag
     4801 tgttaacgga taaagtgatt gataaattgg aaaaagaaaa tccaattgcg gacaatcttc
     4861 gttatgaatt agttgaaaat tctaataatg aatttgttgc tacaaagttt tctgacaact
     4921 tcttatcaac attttctgat agcttgaaat caggctatta ccgataccaa gcaaatctat
     4981 gggggatttc aattgggttg acagcgttag caatcgccta tgtgttcagc gcgtttgtga
     5041 tcattacagc aatactagaa cttgcattta aacgtgtgtt aggtgttctt gtttttgcaa
     5101 cagatattga gacaggacag cgttcaaaag ttgttctatc agatattcta caatgttact
     5161 taactgttgg gtttcaagga tttgggttat caatgtttgc tatgtttatc aattttctga
     5221 actcaggaca aggtatctca acaaatattt ttataaaaac aattgcttat atctgtgccg
     5281 tttttgtttt aatcaaaggc agtgaaacag tcatgagata ctttggtgtt gatattggac
     5341 tcaaagaagg ctatgggcaa cttgcttctg cttttggaat gggcgcaatg ctgtttagaa
     5401 aaggatcaaa tggatttaat cgtgctaaag gaagcggaga aggagaagat cgaaaaccag
     5461 aaaagaattt cggcgaaact ctatcaaaaa aagctggcaa aactggaaga gctttgggat
     5521 atgcccatga acgtggatta tccggtttag cttcagatgg agctacaatg gctagtgaaa
     5581 gagcaacaaa accatttaag agtatgcgtg atatagctaa tgataccaag aataagttta
     5641 aagaaggttt agatgatggt acagtgtccg ctattaataa aaatagcaaa cctatgttag
     5701 ctaaaaataa agaagatgaa actggaaaat atgctgattc cgtgccaaca cgtctttcag
     5761 atagaaaaga tggtgctaaa gttgaaaatg ccgatagaat tatgtctagt tctgaacgta
     5821 tgcgtgaagc aatgaaaaat aatgctgaat cgaataataa tcctgcaagc gcaattcagc
     5881 aaaaagtcca acaagatatt gaagaacgta aaaatgctat gcacggtcaa gctaagtctg
     5941 ctgaagaatt aattaatcag aagcgtcatg aagcgaaata cacgcctgaa gcaatgaatc
     6001 gcgaggaaat aatgttaagg aaacgtgtag aagggcggac aggcgctaat attgacggaa
     6061 aagaagcgtt gaccaaagaa agaattcaag aagctaaaaa ttcaaataca ggattagaaa
     6121 aattagtcaa agaaaatcca caaaattcta caagtagtca tggtggccga tcagtagatg
     6181 tccgagaaaa tattcaaggt tcactgaatg gtcatgttgg acggaatgtt gatgtaagag
     6241 agaatttaca aagttcttca aatgctcaag gtactaagac aattgatgtt cgagaaaatc
     6301 tgcaaagtag cagtacaagt caaccaaaaa cagtagatgt tcgagaaaat gtacaaaatt
     6361 ctacaagtag tcaagggttt aaatcagtgg atgtccgaga gaatgtgcaa ggtagcagtg
     6421 caagtcaacc aaaaacagta gatgtccgag aaaatgtaca aagttctaca agtagtcaag
     6481 gattcaaaac agtagatgtt cgggagaac
//
LOCUS       DACECY010000104         6432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.110, whole genome shotgun sequence.
ACCESSION   DACECY010000104 DACECY010000000
VERSION     DACECY010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.110"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IWO38_002618"
     CDS             complement(99..1229)
                     /locus_tag="IWO38_002618"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ0314639.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IWO38_002619"
     CDS             complement(1878..3080)
                     /locus_tag="IWO38_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0314640.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IWO38_002620"
     CDS             complement(3140..3343)
                     /locus_tag="IWO38_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0314641.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IWO38_002621"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IWO38_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IWO38_002622"
     CDS             4880..5692
                     /locus_tag="IWO38_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0314642.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1930 a         1313 c         1008 g         2181 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcataga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatattttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt ca
//
LOCUS       DACECY010000105         6173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.111, whole genome shotgun sequence.
ACCESSION   DACECY010000105 DACECY010000000
VERSION     DACECY010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.111"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            725..811
                     /locus_tag="IWO38_002623"
     CDS             725..811
                     /locus_tag="IWO38_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ0314643.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IWO38_002624"
     CDS             complement(932..1621)
                     /locus_tag="IWO38_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314644.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="IWO38_002625"
     CDS             complement(1628..1906)
                     /locus_tag="IWO38_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314645.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="IWO38_002626"
     CDS             complement(1866..2780)
                     /locus_tag="IWO38_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ0314646.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IWO38_002627"
     CDS             complement(2762..3142)
                     /locus_tag="IWO38_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ0314647.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IWO38_002628"
     CDS             complement(3592..3879)
                     /locus_tag="IWO38_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0314648.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IWO38_002629"
     CDS             complement(3899..4123)
                     /locus_tag="IWO38_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAQ0314649.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IWO38_002630"
     CDS             complement(4763..5296)
                     /locus_tag="IWO38_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ0314650.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IWO38_002631"
     CDS             complement(5317..6159)
                     /locus_tag="IWO38_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAQ0314651.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACECY010000106         6129 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.112, whole genome shotgun sequence.
ACCESSION   DACECY010000106 DACECY010000000
VERSION     DACECY010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.112"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(173..475)
                     /locus_tag="IWO38_002632"
     CDS             complement(173..475)
                     /locus_tag="IWO38_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314652.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(695..1061)
                     /gene="ssrA"
                     /locus_tag="IWO38_002633"
     tmRNA           complement(695..1061)
                     /gene="ssrA"
                     /locus_tag="IWO38_002633"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1232..2005)
                     /locus_tag="IWO38_002634"
     CDS             complement(1232..2005)
                     /locus_tag="IWO38_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0314653.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2149..2499
                     /locus_tag="IWO38_002635"
     CDS             2149..2499
                     /locus_tag="IWO38_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ0314654.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2480..3196
                     /locus_tag="IWO38_002636"
     CDS             2480..3196
                     /locus_tag="IWO38_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0314655.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3196..4644
                     /locus_tag="IWO38_002637"
     CDS             3196..4644
                     /locus_tag="IWO38_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0314656.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4714..5223)
                     /locus_tag="IWO38_002638"
     CDS             complement(4714..5223)
                     /locus_tag="IWO38_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314657.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5213..5938)
                     /locus_tag="IWO38_002639"
     CDS             complement(5213..5938)
                     /locus_tag="IWO38_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314658.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         2090 a         1062 c         1182 g         1795 t
ORIGIN      
        1 cttataattg gagtggtagt taattagtga cttatttttg attttatagc actgatacta
       61 taaaatatag atatcatcat attacacaat cttaatacta acttaaaaaa tatccctttt
      121 ataagtatgg tgataaaatc cgttccgggc tacctttttt aggtagccta ctttaatctt
      181 tatacctttc tggatcaacg aaagtatact tcaaatagtc ataacgccga tgatcgctcc
      241 gtgcgtctgg cacatcagtc acgatatcaa acaaaaaata cacatctttc ttcattctgg
      301 tttttgttgc cggaatttta aagtagttct tattagagta atagagattg attaataagc
      361 tatcttcgat tgctaaaaag aaaacttctg aatcccacac cttataaaaa tctttgacaa
      421 atctattcga aggatcaaat ttaaaccata attgtgtttt tccttccatc aacataatgt
      481 tcacctcaaa aagagtatac gaactaatgt tctttttgta aagtaggaat atcattgata
      541 atatggatgg aacatatata ttaaaaagtg ttctttttga atgctatttt gaatgccatt
      601 ttcattcatt taacgaaaat ctgcgaaaat aaaaaatgct caaaccctta taaaataagg
      661 atttaagcat ctagcgaaat ctgctgaaat ctcacaatgg agacggcggg agtcgaaccc
      721 gcgtccaaac acatcgccac ttaaaaatct acgttcatag tcaactcatt taaggattca
      781 ctttacagct tgccgagtga caggcattct gtatcgctag tctgatgttc tctttttcaa
      841 cttacagacg gaaagttgaa acgtatccca ctaaagttag gacccttact cgagcacatg
      901 ggcgatgccg agaggatcta cgctaactgt ttttaggcag ctaaagcgta agagttgttt
      961 tcgtttttag cagttatatt taactgtaac gtttttacgt agacgtaacc tacgaaacgc
     1021 aattcaagct cgactgtgcc tgtcgaatcc gtaacgtccc cgttagctaa caagggttac
     1081 gtgtattttt catacaaaat tagtatagca tatattggga taaattcaat aaaaaagcga
     1141 atcatatccc attatttata taataactgc ccctttctta agaaaaaata agcgattttc
     1201 ttaagaaaag aggtagcttc aaaaaaagat cttaaatcag atcaaaatac ttcaatgcgt
     1261 ggacaattcc gccttctgta tttttctttg tgataaaggt agaaagttct tttaatccgt
     1321 cataggcgtt gcccatagcg attttgttgt cgcaagcttc caacaaggca aaatcattcg
     1381 gaccatcgcc aaatccaaaa gtaggaactt tttccagctg aagttctttt tgcagacgtt
     1441 tgacacctgc cccttttgag attcctttct tcactgtatc gatcgagtaa ggaccattcc
     1501 gataaaaagt tagctcagga aaatgttcgt gatagtattc gtcacctgat tgagataaga
     1561 tcagcaacat atttactgtg ttttctttaa agcctaaagg atcaatttcc ggaacatctg
     1621 aatgaatgaa gtcgtaagca ttgatcattg tttggctatg accggtacac cagattttct
     1681 gatcgttata gtaagaaagt tcatgaccgt tcaactgtac atgatcatac attttttgac
     1741 attcttctga agaaaatgtt tgagaataga tttcttcgcc gtctactcga atgaaagaac
     1801 cattcatggt gaccgcagaa gtaatgcccg catcttccat gatagagtgg atttctgctt
     1861 ctgtacgtcc tgtagcgatg acggggagaa cattgttttc ttttaatgcg ttcatagctt
     1921 ttgccacttc gggtgtgatt tttgatttcg catctagtaa tgttccatca agatcaaaga
     1981 aagtgattgc acgataattt gacacaaata aaactccttt tttattggaa taagtttatc
     2041 ttaacaaaga atgaacaaaa gacaaacctt tcgtgaagaa ttcctgaaca tattggttca
     2101 gtcaacaatt tagagtatac tataagaaga gaatttagga gggaaagaat gatttttttc
     2161 agtattggta tcttattctt aataattggc agtctttttc ggattctgcc ctcaaaagga
     2221 aatctgcctt tttatgggta tcattcgcct cttgccgcaa aaacagacgc tcattggcgg
     2281 ctggctcaaa agacaagcgg aaactggttt tttcttatgg gcttactgat ggcattgatc
     2341 ggctattatt taaagacatc tgggcatacg aattattttc tgattgaaat gcttctgcta
     2401 gtatttccaa ttatgcctat ttttattatg acagaaaaga aattacagaa atatgacctg
     2461 gaaactggag ggaacgataa tgaatatttt aatgattgaa gataatcaat ctgtttctga
     2521 aatgatgcag atgtttttct taaatgaagg ttgggaagca actttcaagt atgatggaaa
     2581 agaagggctc gatgcctttt tagaaaaccc acaacattgg gatatgatta cactagacct
     2641 gaatttgccc acactggacg gtatgctagt aggaagagag atccgcaaag tttcaagtac
     2701 tgtgccgatt atcatgctga cagctagaga ttctgaaagc gatcaggtga tcggcttaga
     2761 gatgggggca gatgattatg tcacaaaacc tttcagtcct ctgacgctta ttgctcgaat
     2821 gaaagctttg catcgccgta gtgagttggc agaagcaaga gaagaagcag tagtttcaga
     2881 tgatcatttt gatatcgaga ctagtcattt caagatgaat acgaaaacac gagaagcgta
     2941 tctggacaac cggccaattg aaggactgac gcctaaagag ttcgatctat tgtttacttt
     3001 agcaaaaaaa ccacgtcaag tattctctcg ggagcagttg cttgaattag tctgggatta
     3061 tcagtacttt ggtgatgaac ggacagtgga cgcccatatc aagaaattac gacaaaaaat
     3121 cgaaaaggtc ggaccacaag tcatccaaac tgtttgggga gtcggctaca aattcgatga
     3181 ttctggtgtt gcctaatgcg ttatttgtat cagcaactct tagctttctg cggaatggtc
     3241 gcgttgatct tattgatcgt tggagtttct tttacacaac tgacaaaaca aacgattgaa
     3301 gaaaacaatt accagcaact gtttggatat gctgaatcag ttgaaaagac aacgcaaacc
     3361 tatgccgata cgctcccgca gtttagtcaa gatcaggcgt ttcaaaatgc attgctgctg
     3421 acagagcaag tactgacaga acaaaatgtg aactttatat ttatcgataa aaacgaacgt
     3481 gtcatttatc cgactaatac agctttactg aattttacct taacagctga tcaatggaaa
     3541 aatctgagga atggacgtca agaaaaattc acatcgaata aaaatatttt aggacaagca
     3601 caggcgacct cttacgcact tgtgccattt aatttgaatc atgaattcta tggagcactt
     3661 gtagttagtc aacctgcgcg aaatatcgat aacagcgttc gtccaattac actgaacctg
     3721 ttcaaaggat ttattttttc cagtatcgtt gcagttattg ccagctatgc ttttgcagca
     3781 ttccaagtta agcgtatcaa tcggttaagg aatgcgacga aagaagtgac gaatggtaat
     3841 tttgatgtac agctccccgt acatgacaaa gatgaatttg atgagttagc agatgatttc
     3901 aataaaatga cgaattcatt gaaagaatct caagcagaaa ttgaagaaca agaaaatcgt
     3961 cgaagacaat ttatggcaga tgcttctcac gagatgagaa cacctcttac gacaatcaac
     4021 ggacttctgg aagggttaga atacaatgca atccctgaaa atcagcgaga aaacgcgatt
     4081 aaattgatga aaaatgaaac agagcgtctg atccgtttgg tgaatgaaaa cttggattat
     4141 gaaaaaatcc gtacgaatca aatcagtatg gtcattaaaa aattcaatgg aacagaaaca
     4201 ttgaaaaata ttgttgcaca gttagaagct aaagcagaag cagcaggaga tacattgaca
     4261 ttgaaagccg ttgatgatat tgatgtttac gctgattatg accgatttgt ccaaatcatg
     4321 gtcaatatta ttcaaaacgc gatacagttt actgaaaacg gccaaatcac gattacttta
     4381 gaaaaaggct atcttgaaac gatcatcacg attgaagata cgggaattgg aatgtctgaa
     4441 caacaaatga aaagtatctg ggatcgttat tataaagtag atccttcaag aaaaaataca
     4501 aaatatggtg aatcaggatt aggtttacca atcgtgcagc aacttgtacg cttgcataaa
     4561 ggaaaattag aagtagaaag cgaactagga aaaggaacaa aattcgctgt tcgtttgcct
     4621 gacgtagaaa tcaaaaaaga gtaaaaaaca agcggtataa aagatcatga atcgatcttc
     4681 tataccgctt gtttttacta taaaatgatt gatttagccc gcgggctcta aacgatcaat
     4741 ataaggactt tgagcaatag tagcatcatg agtaaagagt aagatacctt gattttcttc
     4801 atgagctaaa taagaaagca agtggagtag ctcttggatc tccaatacac taagttcatc
     4861 aaatgtatct tcaataacaa tattgtttcg cttggaaagc aaggcatgaa gtaattgcag
     4921 ctttactttt tcaaaagacg tcaactcagc tgccgcacaa ttaagcaagg aaggagagat
     4981 atcaagtttt tcttgatagg ccaaacgatc ttgtttgatt tctttttccg gtacatccaa
     5041 caggacatgg tctttaagat tcagataagg aaacatcggc caattttttt ggatgatcgc
     5101 aaaattatcc agttttgcaa caagaacctc ttttttcaag tgctccaata ttttatgctg
     5161 tttttcgaga ggacacaaaa taacggtcaa gtgttcagca tgaatcaaat attcaggttt
     5221 cattacgtcc aggcctccta ctttgcaaat gatggataaa aaaagtgaaa gggaatagac
     5281 taagaatgag acagcatcca ctgagcccaa taagagtttg ggcaaatccc ttaagtatat
     5341 ctgtgaaaaa aagatcattt tggaaatcga tggtaaaaaa agtctgttga tccatcggaa
     5401 ttgtaagtcc gcttaccgaa gtcaagatat cacggatatc tggcagttca gtagtcttta
     5461 agacaagaaa attgatcctt tgaagaagcg cttcataaac tgactggaag cttacgatcc
     5521 acaataaaac caagatacaa caaagaaaga gaggaaagaa cagctgccag aaaataaaaa
     5581 ggaataattt ggttttagaa agaccagtca ggtgccaatt gatcaattcc tgcttgtttt
     5641 gcctgactga atagtaaaag aaaaagataa aaaggaaggc aaatagaaaa aataaactga
     5701 atatgccttt tatataaaat cggctagcta attctaaagc cggaagaagc tctgtagatg
     5761 cagaagcgcc aataaatgaa agccgatctt ggacttgatt ggctaaagcc gattgcaaat
     5821 ggtaaagagt caggcatgct atcaataaaa aagcagaaaa gcttgaaaat aaacaataga
     5881 gtattgtctg ctttttgtaa taaattaatc gagcagtagc atagcggatt attttcatac
     5941 ttttcacctc tataaaaagt atagaaaatg aatatgaata aagtatgaaa ttcctttagt
     6001 gaaatcacga attaaaacca gaaaatattt tgtaaaaagc aagaggttgt gaagaagctg
     6061 ccctattcta ttaccgtaga tgcaagcttt tgaacactgt ttattcgaaa agaggagcag
     6121 cgacaataa
//
LOCUS       DACECY010000107         6116 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.113, whole genome shotgun sequence.
ACCESSION   DACECY010000107 DACECY010000000
VERSION     DACECY010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.113"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            174..1478
                     /locus_tag="IWO38_002640"
     CDS             174..1478
                     /locus_tag="IWO38_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0314659.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            1574..2443
                     /locus_tag="IWO38_002641"
     CDS             1574..2443
                     /locus_tag="IWO38_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0314660.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(2555..2875)
                     /locus_tag="IWO38_002642"
     CDS             complement(2555..2875)
                     /locus_tag="IWO38_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ0314661.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            3045..4004
                     /locus_tag="IWO38_002643"
     CDS             3045..4004
                     /locus_tag="IWO38_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0314662.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            4094..4489
                     /gene="dhaM"
                     /locus_tag="IWO38_002644"
     CDS             4094..4489
                     /gene="dhaM"
                     /locus_tag="IWO38_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ0314663.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            4491..5474
                     /gene="dhaK"
                     /locus_tag="IWO38_002645"
     CDS             4491..5474
                     /gene="dhaK"
                     /locus_tag="IWO38_002645"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ0314664.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            5502..6107
                     /gene="dhaL"
                     /locus_tag="IWO38_002646"
     CDS             5502..6107
                     /gene="dhaL"
                     /locus_tag="IWO38_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ0314665.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
BASE COUNT         2054 a         1103 c         1325 g         1634 t
ORIGIN      
        1 tatgaagatg aaaaattgaa aaaaagacaa gaattacttg aagaagtgta tacggaacgt
       61 tttggaacaa aagaagagag agaaacggtc cgtttttatt ctgtctccga agagaaaaat
      121 ctagatacga cattcattgc agatttgtat aaagaaaatg gggtggaact gaaatgatct
      181 caatcaccca tacaggtaat ttattcttag atacttgttt gtctattatg tatttttttc
      241 tcgtttctta tccgatttta ggcggatttg tatggtttat tggtgtctgg tgttatgtgt
      301 ttttatacaa acataagcaa aaagaatggg tagatgttcc cttgagtgtc gaaccgttca
      361 tcacgatcat ggtgccagcc cataatgaag aaattgtgat cgaagataca atcgagtatt
      421 tgatgacgaa attgaattat cacaactatg aggtattagt gacagacgat ggttcaacgg
      481 accaaacacc agaaatatta gctagactga tgaaaaaata cgctaatctt cgtgtggttc
      541 ggatcgaaaa aaataaagga aaagcccacg cgtttaatat cggacttgcc tttgcaaaag
      601 ggaaattgat tttaagcaat gatgcagaca cggtaccgga gccagatgca ctgattaggt
      661 atgtgaatta tttcattcgc cctggtgcaa gacatattgc tgcggtcact gcaaatatgg
      721 atgtccaaaa tcgtaccaaa ttgattgcaa aatctcaaac ggttgaattc tcaagtatcg
      781 tgggcatcat taaacgaaca cagtcagctg ttttcggtgg tctttatgcg tacagtggag
      841 caaatacgat gtatcgaaaa gaagccttga tcgatgtggg cggattcaga caagatcgag
      901 cgacagaaga catcagtatt gcttgggatc atcagttaaa tgactggtta tctgtttttg
      961 ctcctgagat tatctttttc atggaagtac ctgtgacgtt gaagatgcta tatcgacaca
     1021 gaaaacgttg ggcaaaaggt ggaacagaag tctggctgac gaatttcaaa aaagtgatgc
     1081 tccatccttt taaacatatt ggaagaacga tcatcttcat tgatcaaaca ttgagtattg
     1141 tctggtcaat attcttttgt atttccgtag ttctttttgc tggattaatc gggcattatg
     1201 tatatcaagg aaactacgaa cagatttata ttacttttac attcagtttt gtgttcatct
     1261 gctttgaaat ggtcgcagga tttttccagt tgctagcttc gctgattgta gatgatcgac
     1321 gaagaaaatt gaaatacctt cttttcgcac cactatatat gcttttgttc tggatagtga
     1381 acgcgataac gatcgtgacg actttcattc cagcagtcaa aacgatttta ggttatggaa
     1441 gcgggacatg gaaaagcccg gaacgtacaa aaaaataaaa gatgcaccgt tttttagaca
     1501 aacagcctgt tgtgtttatg gttcttccaa acatttttgc ttataataag aaattagtta
     1561 aaaagtagta aggatgggaa aagtgatgaa aaaaagaatg aagccgttaa tctggccaat
     1621 tgctgtttcc agtattttct tattgggaca gacagctttt gcggaaactt ctgaacctgc
     1681 tcaaacagaa ccttcaacaa ccataagctc ttctggtgct gaaaacggtg cctcggaaac
     1741 agaaatgact tccaatgtac cgacagataa ttcaggagaa acgatttcat cttcagaaga
     1801 tacatcaata aatagttcta cctcttctga ccaaatcgat catttgcaag acttggaaaa
     1861 taaaagaaaa gaactcgaag aagcattaga agctgcaaaa aacaaattga aagaagacca
     1921 agcagcaatc gatcaagatt tagctgatct taaaaataaa gtgggaaatg accgtgcaca
     1981 acttgagaaa aagcttgccg aagcgaaagc aaaagcggaa gctgtccaaa cagatcttga
     2041 cgggaagatc caacaagcaa aagaattcaa tcagcaatta aatcaggttg ctcaaacagc
     2101 ttctggaaag ctaagtcaaa ctcaagcgga tatcacagcg gccagtcaag caatccacgg
     2161 aaagatcagc gaagcacaaa cagttgctaa tgattttgaa gcaaaaaaag ctgacttagc
     2221 agctagtttc caacgaataa aagatatttt gcattcggca ggagaaaaat cagtgacctc
     2281 tggaagcacg acagaaacag ttcagcaaac gacagagcca tccaaatcaa caagtcagac
     2341 tttgccaaaa acaaatgaaa aaacagcttc tgtcagttat tccttagcag gggcgggtgt
     2401 gttagtcgca gcagcttacg gaatcatccg caaaaagaaa taaaaaagag aggatgggaa
     2461 ggtagagtac ctcgaagtta gactgaaaaa tctaattctt gagtgccact acatgtccca
     2521 tctctcttta gttattaaaa cagatgtccc ctacttatct cgtggagaca gctgttccac
     2581 tacatgtgac catcagcatg ccattgtcgc tgcctaatac ttcatagtcc atttttacgc
     2641 caatgatcgc atcagctcct aatgcttttg cacgttcggc catttcggca agtgcttctt
     2701 ctcttgcacc cattagttcg ttttcatagc tttttgagcg gccgccaaag atatttcgca
     2761 accccgcctc caagtcttta aatgcatgga tcccagtaat gacctcacca aaaacgatcc
     2821 ctttataagc tactacggct tttccctcta cactgttcgt tgttgttgta atcattcgat
     2881 cactcctctc ttttaattaa agtataacaa acttcaagaa gcactacaca tattttccca
     2941 aaattagaaa tcgccgattg taaaattaag ctagacaact aaaaaagtca tttgtgctac
     3001 actcaaaata gttccgtcga aagaattata aggagtgagt acaaatgacc tatacccatc
     3061 ttacaccaac tgaactcgta atgatagaag cttatttcaa tcaatcacac tctgtttcaa
     3121 aagtagccca tctggtacaa cgttctcgac aaaccattta taatgtctat cgttttctta
     3181 aatctggtgg aagcgcaata agctactatg aacaatataa gaaaaacaaa cgtaactgcg
     3241 gaagacgtcc gattgtgtta ccagatgggc aacaagaata cattcaaaaa aaggtggctc
     3301 aagggtggac tccagatgtc cttattggtc gagcagagtt tcctattgct tgttcggtac
     3361 gtacactata tcggatgttt aaagataaaa cctttgaccc tcacgattta ccaatgaaag
     3421 ggaaaagaaa accaaacgga cataaagaaa aaagaggaaa acaagctttt cgccgttcga
     3481 ttcaggatag aacaactgat tatatacagt tcaatcatga gtttggtcac ctagaaggtg
     3541 acaccattgt cggtggaaaa cataaaagtg cggtgatcac gttagttgag cgattatcta
     3601 aagtgatcat caccttgaaa cccgctggta ggcaagctat agatattgag aatagactga
     3661 atcaatggtt tcaatctgta ccgaaacact tgtttaaatc catcacattt gattgtggaa
     3721 aagagttctc gaattggaaa aagattagta ataccaatga tatttcgatt tatttcgctg
     3781 atccaggaac accttctcaa cgaggactaa atgaacattc gaatggctta ttacgtaaag
     3841 atggcttata taaagctatg gattttaata aggtatccga aacctttatc caatcggtgg
     3901 catcaaaacg aaatcatatt cctagaaaat cattacacta tcgcacacca ttggaagtat
     3961 ttttgagtta cgtaggtaaa gatgatttgt ctagcttaat ttgacaaacg aaaaatttat
     4021 tatatacttg aattgttcat taatgaaagc gaaaccaatc gttagcaact atgtaaaaga
     4081 aaggaagatt cttgtgaaaa aaagtatttt gcttatttct catagtcaaa aagtaacaga
     4141 tggtattaaa gaaatgattg agcagatgca aaaatccgaa gaggtgcaaa ttttttctct
     4201 cggcggaata aatggcgaga tcggatcaga cccgatgaaa atcgtagaag cagtcaatga
     4261 atcaagcgat gcagctagtt attttgtttt tgcggatatc ggtagcgcgg tattgaactc
     4321 ggaactggca aaagacatgt tggaagaaga gcagcagcaa cgttatcatc tggtggatgc
     4381 gcctttagtg gaaggagctt ttgcagcagc catcactgcc ggtataacag atgatactca
     4441 gcaaatctta acagaagcac aaaatgccgg aaagaaagga tgggaataaa ttgaaaaaaa
     4501 tcatgaatga tccaagcaat attgtcgaag aaatgctgga aggattagta aaaagctatc
     4561 cagaacttgt tcatcgtgta gaatcttccc gtgttgtagc caagaatcag aaagcggaac
     4621 aagttggttt ggtttctggt ggaggaagcg gacatgaacc atcccatgcc gggtttgtag
     4681 gggaagggat gttaagtgca gcggtgcttg gtgatgtgtt tacttctcca actccagatc
     4741 agattcagac ggctattaag gaagccgata gtggaaaagg agttttgctg atcgttaaga
     4801 attatacagg ggatgcattg aacttcgata tggcaaagga attagctgct atggacgata
     4861 ttgaagtaga aagtgtgatc gtagatgatg atatcgccgt tgaaaatagt acgtatactg
     4921 ctggaaaacg cggagttgca ggtactgtcc ttgtacacaa aatcgtgggc gatgcagcaa
     4981 gaaacggtgc ttccctagct gagttgaaag aactgggaga aaaggtcgta caggcaacta
     5041 agacgatcgg tctagcgctt cgagcagcaa ctgtaccaga agtagggaaa ccaggattcg
     5101 aactaggaga agatgagatc gagtatggtg ttggtatcca cggtgagcca ggctatcgtc
     5161 gagaaaagat gcagccttct aaagtactgg caaaggaact tgttactaag attttagatg
     5221 attactcgaa tttaccaaaa gaagcaggtg tactagtcaa cggaatgggc gggacgcctt
     5281 tgatggaaca gttcgtgttc atgaatgatg ttttagctct gttggaagaa agaggcgtca
     5341 atgtagtttt ccgaaaagtc ggtaatttta tgacctcact agatatgcaa ggtctttctc
     5401 taacactgat cgacttgacg gagacacaat ggaaagattc tttagaaagc aatgtacaga
     5461 cgatttcatg gtaaaaaatc tcaagataaa agggtgataa tatgaaacta accgttcaag
     5521 aaatccaaga atggctcacg caatttgcag aggcaatcaa tcagaacaaa caatatctca
     5581 gtgatctaga taccccgatt ggcgatggag accatggaaa taacatggga agaggggttt
     5641 cagcttatga agaagctttt caagccgatc atcctgaaac aatcagcgat acattcaaag
     5701 tattctctat ggcgatgata tcgaaagtcg gtggggcttc tggtccgtta tacggatctg
     5761 cattcatgaa catgatgaaa gcgacaaaag aggtcgattc tattgattct caagaaaaat
     5821 taggcgaagt gatcgagcag ggaacagcgg gtattcaaag ccgaggaaag gcagaagctg
     5881 acgataaaac gatgttagat gtttggctgc cagtcacaga ggcattgaag gcagggaatt
     5941 taacaaaaga aatcattgac caggcaaaag aacacaccaa agtcttggta gctaaaaaag
     6001 gacgagcttc ctatctagga gagcgtgcaa tcggacatat cgacccagga gctgcttcta
     6061 gtgccatttt attttcaacg cttttagatg tgatcaatca accttaagtt caataa
//
LOCUS       DACECY010000108         6068 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.114, whole genome shotgun sequence.
ACCESSION   DACECY010000108 DACECY010000000
VERSION     DACECY010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.114"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            832..1566
                     /locus_tag="IWO38_002647"
     CDS             832..1566
                     /locus_tag="IWO38_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0314666.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="IWO38_002648"
     CDS             1582..2751
                     /locus_tag="IWO38_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0314667.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="IWO38_002649"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="IWO38_002649"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ0314668.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="IWO38_002650"
     CDS             3841..4767
                     /locus_tag="IWO38_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ0314669.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="IWO38_002651"
     CDS             4786..6036
                     /locus_tag="IWO38_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0314670.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2027 a          997 c         1246 g         1798 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacaa caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agttaagt
//
LOCUS       DACECY010000109         5982 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.115, whole genome shotgun sequence.
ACCESSION   DACECY010000109 DACECY010000000
VERSION     DACECY010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.115"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..450)
                     /locus_tag="IWO38_002652"
     CDS             complement(<1..450)
                     /locus_tag="IWO38_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0314671.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            674..1465
                     /locus_tag="IWO38_002653"
     CDS             674..1465
                     /locus_tag="IWO38_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0314672.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1431..2384
                     /locus_tag="IWO38_002654"
     CDS             1431..2384
                     /locus_tag="IWO38_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HAQ0314673.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2782..3756)
                     /locus_tag="IWO38_002655"
     CDS             complement(2782..3756)
                     /locus_tag="IWO38_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0314674.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YNAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3871..4305
                     /locus_tag="IWO38_002656"
     CDS             3871..4305
                     /locus_tag="IWO38_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0314675.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4430..5416)
                     /locus_tag="IWO38_002657"
     CDS             complement(4430..5416)
                     /locus_tag="IWO38_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAQ0314676.1"
                     /translation="MEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGAFFRVHHFAPQIG
                     SPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQGRVQLGVGRGSPE
                     QVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPMFPQGSGKLRIEP
                     YSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQQANQLRAYKEAW
                     KQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYHRNPTIFGKTFAG
                     EPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIAPELGWR"
     gene            5844..>5982
                     /locus_tag="IWO38_002658"
     CDS             5844..>5982
                     /locus_tag="IWO38_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HAQ0314677.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PR"
BASE COUNT         1880 a         1128 c         1031 g         1943 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaag ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgctttcaa aagaaattta ttttgttaaa tttgatctac ttgaaaataa
      601 aatcgtttat ctatataatt aagaagaaca aagtcgaaca aaaattgact tttatgttaa
      661 aggagtaatt catatgctga aaaaagaaag acaagaacaa atcattacat tattagagaa
      721 agatttttat ttaactaatt cagaaatcgc taacaaatta ggcgtatcag aaatgaccat
      781 acgccgagat gtcactgaat tagcaaaaaa aaataaagta aaaaagctat atggtggcgt
      841 tgaacgaaag gaaccttata atcgagaact atcgactcaa gaaaagattg atacgaatgt
      901 agccgaaaaa aaatatattg gtcaagtcat gaacagtatg attcccgata attctactat
      961 ttatgtcggt gctggaacaa cgatcctata tgcacttcct ttcattcaaa agaaaaatct
     1021 atttgtaatt acaaacagtt tgattgcttt caactattta aagaatcata caagttatcg
     1081 gattttactt actggtggcg aattttctcc tattacagaa gagttcattg gggaagttgc
     1141 agaaagatct tttgaaagtt tgaatgtcga tatctctttc gctgcaacaa acggcatatt
     1201 tgataacaat gtgacaaccg ctcaatttat tgagggaaat attcagaacg cagcgctcaa
     1261 tgcagcaaaa atcaaatgca ttgttgcaga ttattcaaaa ttaaataaat cagacgtcta
     1321 tacttttaga aaattaaccg attttgatta tctaataacc gataatcatt tatcagaaca
     1381 ggatttcaat cattattcaa aatacttaac aatattaaag gagcccaaaa atgatactga
     1441 caatcacgat gaacccatcc attgattcca tttattttac aaaacacttt gaattaggta
     1501 cgatgacacg ctttgattct ccggtacgct atgtaggcgg caagggaatc aattgcggac
     1561 gagcttctgc tctactaggt tcatcagtta ttttaacagg gtttttgggc ggttattttg
     1621 gcagtatgat tcaagatatg ctgaaaaatg aaaatttgtt tgatttgcat tttttaccta
     1681 ttgcagaaga atcaagaggt gccgtcacga tcatgcatga tgatgaaacg cagacagagc
     1741 tagtggaagc tggtcctgaa atagcagata ctactatcaa aaaattaatg aatgatatca
     1801 aactaatcag taaaaataat tacctttcag tcattacaat aaatggttct gttaacaatc
     1861 cagatccctt tttgtatgct aaattattac aatatatcaa agaacaaata gatccttcca
     1921 cctatatatt aatggatata tctggacaac atctccgtga agtcttagca agtaagtata
     1981 aaccgatgtt catcaaacct aatatccatg agttttcaga tatcatccat caaaaaatta
     2041 ctcaaaaaag cgacataaaa caccatctaa aaacaaatcc actatttaaa gatatcgatg
     2101 ttatcatgat ttcttgcggc tcgtatggtg cagtagtcaa agtaaaagaa gaattttgga
     2161 atgttgagat ccctaagatt cctatcatta atacaacagg ctctggggat gccactgttg
     2221 gaggttttgc tcattcatta gaacagaatt atcctttaaa agaatgtgta aggtacgcaa
     2281 tggcttgcgg catgtcgaat gcacagcaat cagaagtcgg gaaagttatt ccagctgatg
     2341 taaaaaattt agcaaaaagc attaaaatca cagaaatgaa ataaatagta caaagtaaat
     2401 aattgtttat atgaaatgag acacaaagag actgtgacaa aaaacgtgtg taccgacccc
     2461 caaaagttag attttttggt ctaacttttg ggagtcgata cattgtcaca gtcctttttt
     2521 cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
     2581 gagaagctat tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacgacc
     2641 tcttggtata aacggtgttc aaaagcttgc atctacgcta atgagtccaa ccaagtcaaa
     2701 aatgaggagc ttttttggct gtccttatta cttgatgcag ggcagttttt tcataatctc
     2761 ttttagatta aatccttttg gttacacaag gttctcttca aagaatgttg ccattttttc
     2821 aaatggaatg atgtctgtct ggtcgtatag atctgtatgg gatgctcctg gaatgatcat
     2881 gagttccttg ttgtcacctt ttagttcttt gaaagcgtcc tccccaaagt aacgtgaatg
     2941 agctttttcc ccgtgaatga ctaagactgc atgacggatt tctggaatat aatgcaatag
     3001 ttttgcattc ataagtgaca accctgatgt cctattccaa ccatcattgg agtttaaaga
     3061 acggacatga tatccgcggt ctgttttata atacgcatta tagtctttga caaattgagg
     3121 agcatcttca ggcaacggat caaccacacc tccagcacgt gcgtagtccc cttgtttgaa
     3181 atcaatgatg cgttgtgcat ttaattcttc acgcatttca tatcgggcat tttcatccat
     3241 aacatcgaag tagccatttg ctgttactcg agtcatgtca tacattgtgg aaacaactgt
     3301 tgctttgacc cgcgtatcga tagctgctgc attcaatgcc atacctcccc atccacaaat
     3361 accaatgatt ccgattctct cagggtttac atttttttgt acagataaaa agtctactgc
     3421 cgcttggaaa tcttccgtat tgatatcagg cgaagcaaca tatcgaggta ctcctccgct
     3481 ttctcctgta aacgatggat caaacgcaat cgttaaaaag cctctttcag ccatcgtctg
     3541 cgcatataga ccagacgatt gttccttcac tgcaccaaat ggcccgctca cagcgattgc
     3601 cggcaatttc ttttcactta cttcttttgg ttcatataaa tcagcgacta atgtgatacc
     3661 aaaacgattc gggaagctca cctttcgatg attgacttga ttgctttttg gaaatacttt
     3721 gtcccattct tgtgtgatgt ttaattcttt attcatattc tagactctcc tttgatcaaa
     3781 attgacattt gtttttgaat gtattattat gataaaactt aaacttgact ttaggtcaag
     3841 gtaaaaattt tattttagga ggattttttt atgtattcca ttggacaagt gtctgaaatg
     3901 tttggattgc ctgtttctac tttacgttat tacgacaaag agggattatt tcctgatatc
     3961 cagcgtgttt ctggtattag aaaattttca gagaatgagt tagaggcttt aagagtcatt
     4021 gactgtttga aaaaatctgg tttagaaata aaagacatta agcaattcat ggaatggagc
     4081 aaagaaggca gtaagacatt tgaggttcgt aaagaactat ttgaaaaaca aaaagaagta
     4141 gtagaaaaag aaatagcaaa attagaaaga gtactcgcta tgctaaacta caaaagctgg
     4201 tactatgaag aagcaataaa agccggcaat gaagaagccg tattgacaat gattcctgat
     4261 gatttacccc aacatgtaaa agaagcttat gtacattctc attgaacaaa cggataactc
     4321 actctccttc tctatgaact tcatgaaaac atgaataaac acctctaact gattgttaga
     4381 agtgtttatt ttttcactca agtatcctgc acttacaaaa atatcctttt taccgccatc
     4441 ctaattctgg agcgatatct tttacgattg tttcaagcaa gtgcatgtta tattctaccc
     4501 ccaaagtatt aggaatcgtg acaagtactg tgtctgcttc tttgattgct tcatcttcag
     4561 ctagctcctt gattaattga tcaggttcac cagcaaaagt ttttccaaaa atcgttgggt
     4621 ttcggtgata agcaagatag ccgacagcat cttgttgcgg actaccgtct tgtccaaata
     4681 agcgtcgatc caaatcagtg gtgattggct gaatcgagcg agtaactaat gttcttggtt
     4741 caaaatcatg acctgcttgc ttccacgctt ctttatacgc acgtaattgg tttgcttgtt
     4801 ggatatggaa tggctcgtgg gattcgtaat tgactaatgt agatgattgc agattcattc
     4861 catgttgtgc tgcccagatt gccgtttgct gcgaaccagc tccccaccag attctttgtc
     4921 gtaatccttc tgaataaggt tctatccgta attttcctga tccttgtggg aacattggtt
     4981 gaggatttgg ttcttcaaat ggttttcctt caagtaattc caagaattca agggtgcgtt
     5041 cccgtgttac ctctttgatt tcttcttcac tgtaatcata accaaagtat ttccagccat
     5101 ctagaacttg ttcaggtgaa ccacgcccca ctcctagttg cactcttcct tgcgtgatga
     5161 tatctgtcaa tccggcattt tctgccatat aataagggtt ttcatatcgc atatcaataa
     5221 gtcctgtacc gatttcaatc ttacttgtct ttgcgccgat tgccgctagc aaaggaaacg
     5281 gcgacccaat ctgaggggca aaatgatgta cgcggaaaaa cgctccgtcc actccgattt
     5341 tttctgcttc caccgctaaa tcaatcgatt gcaaataagc atcttgagct gatttcacaa
     5401 gcgatccttg ctccatccaa tgtccaaagc ttaaaaagcc gatttttttc aaggtaatga
     5461 cctcctttaa acctacaaaa atggaaggtt ttttattttt aatatgaact gattctacta
     5521 ctttttttct taaaagacga ataattgttc tcgcctcaca atcaagaaat gtatcggctt
     5581 actacacccc tactttcttc atcatctgcc gtgtcttaat ctcttttaac agtctgttac
     5641 atcgttgatt tttgactcaa aatgattgtt tctaacatgt atttatggta aaaaagacaa
     5701 gattagttct ttgtacacat cgatttatac gctgaacaaa aagaaatata taaactaaga
     5761 aacccattgt tttttcgctc catcacatag agtttacttt agataaacag tgacatttac
     5821 taatccataa cggaggaaat caaatgaaaa aaatctattt cttatgtacg ggcaattcat
     5881 gccgcagtca aatagcagaa ggctacgcgc gtaaatactt gtctccttca atgtttgaga
     5941 taagaagtgc ggggatcgag acacatggct tgaatccaag ag
//
LOCUS       DACECY010000110         5925 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.116, whole genome shotgun sequence.
ACCESSION   DACECY010000110 DACECY010000000
VERSION     DACECY010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.116"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(76..1170)
                     /locus_tag="IWO38_002659"
     CDS             complement(76..1170)
                     /locus_tag="IWO38_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0314678.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1359..2120
                     /locus_tag="IWO38_002660"
     CDS             1359..2120
                     /locus_tag="IWO38_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0314679.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="IWO38_002661"
     CDS             complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="IWO38_002661"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ0314680.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2895..3650
                     /gene="fabI"
                     /locus_tag="IWO38_002662"
     CDS             2895..3650
                     /gene="fabI"
                     /locus_tag="IWO38_002662"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ0314681.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3760..4773)
                     /locus_tag="IWO38_002663"
     CDS             complement(3760..4773)
                     /locus_tag="IWO38_002663"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ0314682.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            4938..5858
                     /locus_tag="IWO38_002664"
     CDS             4938..5858
                     /locus_tag="IWO38_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ0314683.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1806 a         1181 c         1063 g         1875 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
     1261 ttaaaataaa tgataaattc caataattta agtaggtatt ttccctctat tttgttatca
     1321 ttaaagataa caaaatagta aattggaaag gaacagttat gaagaaaaat aaagttattt
     1381 taagtattag tttagcagca gcattgctgt ttgctgctgg ttgttctgca aacaacacga
     1441 cttcttctag ttcgtctaca gaaagttcaa aaagtacatc tactgcggaa agcacgactg
     1501 ccagtagcgt cgatttgaac gcattggaat taccacaatt gacgaaagat gtcgccgaaa
     1561 atgaagatct ggtcgaaatg gtgacgaata aaggcacaat caaaatcaaa ttatttcccg
     1621 aacacgcacc aaaagcggtt gaaaacttta tgacacatgc aaaagacggt tactacaacg
     1681 gactgacttt ccatcgcgtc atcaaagatt tcatgatcca aggtggcgat cctaaaggcg
     1741 atggcactgg aggggaaagc atttggggag aaggatttga aacagagatc aacaaccacc
     1801 tttataatat aagaggtgcg ttatctatgg ctcgctcaca agatcctaac agtaacggca
     1861 gccagttctt tatcgttcaa aatacagatg atatgcatga tggtctttta aaagacgact
     1921 atcctcaagc aatcatcgac gcttataaaa acggcggtta tccttcacta gatggccatt
     1981 atactgtctt cggccaagtg attgaaggaa tggatgtcgt ggatgctatt gcaaatatcg
     2041 aaacagatag ttcagacaaa ccaaaagaag aagtaaaaat cgataaaatc aatatactgc
     2101 aagaagcaaa acaaaaataa aaaaataacg tctgaatcct gcatagatga caacgtatgc
     2161 taggattcag gcgtcttctt tttacataaa aagcaagtaa gcttcctttt gttttgctat
     2221 atcaggtttt atagtcaagc attttggttg acgatgaacg tgaactcgca ctcacagact
     2281 tttttatcat ctacatatgc tgcagataac gctgtaccga ctgatccctt cattttggtg
     2341 atttcaaaat gacatttcag cacatctcct ggaacaactt tttgacggaa tttggcctta
     2401 ttgatcccac cgatataagc tctttttcct agaaactctt ctgattttag gataagtatc
     2461 gaacctgctt gtgccagtgt ttctagaatc aacgcacccg gcatcactgg attgtcagga
     2521 aagtgacctt ggaaaaaatc ttcattgatt gttacatttt ttgtcgcaac gatctttttt
     2581 cctggttcta aagtatctac ataatcaata taacaaatag gataacgatt aggaatcaat
     2641 tccattactt cagtagcaga taatacgtaa gacataatta aactccttct ttcaggcaat
     2701 tgccattttc attactttga ctatcaaagt atcatattcc taaatattta gcaatctttt
     2761 tttaatttaa gaaaaaatag tttgacaatc aaaacatttt gactaataat taagaaatca
     2821 agaagttatt ttagctatta ttctctattt ttttacgact atcaaatttt tatcagaaac
     2881 aaaggagagt cattatgtca tttctagaag gaaaaaaaat cgtcattatg ggcgtcgcta
     2941 acaaaaaaag cattgcttgg ggttgtgctg aagcaatgtt gaaacaaggg gcagaaatta
     3001 tctatactta ccaaaatgaa cgaatgaaaa aatcattagt caaattggtc ggcgaagaag
     3061 cattcactgt agaatgtgat gtttctagcg acgaaagcat cgaaaaagca atgaatgcca
     3121 tcggtgaata tgctggggag atccacggcc ttgtccatgc agttgcttac gccaacaaag
     3181 aagaattgtc cggaaatgtg tctgatattt ccagagacgg ctatcaatta gctcaagata
     3241 tcagcagcta ctctttaatt gctgtatcaa aatacgcaca accgtttctt gcaccaaaca
     3301 gcgggatcgt tagcatgact tatctaggtt cagaacgcgc aatcccgaat tataacatga
     3361 tgggtattgc gaaagcttct cttgaagctg ctatccgtta tttggcagct gagttctcgc
     3421 ctaaaggaat tcgggtcaat ggaatctcag ctggggcaat caaaacatta gctgtcacag
     3481 gtgttaaaga ctatcaaaaa ctgatccagt tatctgaatc tcgtacgcct gatcagcaag
     3541 gtgtgacgat cgaagaagtc ggtaatgctt gtgctttctt aatcagccca ctttccagcg
     3601 gaatcatcgg tgatattatc tacgttgaca aaggtgtcca tctttcataa acttttgttg
     3661 cattctttaa agtaaaacat ataaaaatca ggaaaaaact ttttaaagtc tcttcctgat
     3721 tttttctttt tacgtttttt tcaactcatt actttgaaat tattccacga cttcaatcca
     3781 gccttctggt gcttctatgt ctccaaattg aatacctgtc aattcatcat acaaccgttt
     3841 tgttactggt cctacttccg tttcactata aaatacatga agattgttat tgtgataaat
     3901 cccaccgatt ggagaaataa cagccgctgt accacatgca ccagcttctg caaattgatc
     3961 tagatcatca atgaaaatat ctccttcaat tgcttgcagt cctagtcgtt cttcagctaa
     4021 ctgcaacaag gaatatttcg tgatacttgg taagatagag ggcgattgcg gagtgatgaa
     4081 ttttccgtct tttgtaattc caaagaaatt tgctgcacct acttcttcga ttttcgtatg
     4141 tgtggctgga tcaagataaa cacaatctga gtaatccagt tcttttgctt ctgttcctgg
     4201 aagtaaactc gctgcgtaat ttccgccgac ttttgctgca ccagtcccct taggagcagc
     4261 acgatcgtaa tcagatacaa taaaattcgt tggttttagt ccacctttga aatacgctcc
     4321 tactggcgta cagaagacga caaataaata ttctggagct ggatggacac caatgttttc
     4381 accgacccca atcagtaaag ggcgcaagta taatgtagcg cctgttccat agggcggcac
     4441 aaaatcttca tttgctttta ccacttgctt gacagcatca acgaacatct tttcagggac
     4501 cattggcatc agcaatcgtc tagcactttg attcatccgt cgactatttt gttcaggtcg
     4561 aaagagattg accttgccgt cttttcgacg ataagctttt agaccttcaa aacattgctg
     4621 accataatga agcgctggag agccttcatg aatcgtcagg taattatctt ctgtcaattc
     4681 tccttcttcc cattgtccgt ctttccattt ggcaataaaa cgccaaggag ttttgatata
     4741 agaaaaacct agattgttcc aatcgatttg catatactta ccaccttcgt tattttctcc
     4801 acattttatc attatccata ttatttgtca ttttattttc agcttttttc cttcattata
     4861 gttatttttg ctatttcctt ttttgaaaac ttcttggtac aatacagaaa gaatcaaatt
     4921 ttagaaaaga ggattttatg ttttcattat tagcaactgc cggtacgaca gactcttctg
     4981 gcgagacatt gacagatcaa gctgtcagca aagtaaatgc gtttcagcgg ttttgggacg
     5041 ggattgattg ggacgccatt ttggctgcct ttatccaaaa aagtttatat ctgatttttt
     5101 tgatcgtcct tttttggatt cttcaccgtg tgggaaaata tctgattgat aagtctttta
     5161 atcagtattc taaaaaagcc attctaaacg agagccgctt aaagacttta catagtcttt
     5221 taaatactat ttttcattat acccttggtt tcttttttat ctatgcaatt ttatctgcta
     5281 tcggtgtgcc gatcggatca cttttggctg gtgccggaat tgcaggtgtg gcaatcggtc
     5341 ttggtgccca agggttcatg agtgatgtta ttaccggatt ttttattatc atggaacaac
     5401 agatggatgt tggggactac atcaagctag caaacttaac gattgaagga accgtcgtat
     5461 ctgttggtat ccgtaccctt cagctgaaag caacggatgg tactgtccat ttcatcccga
     5521 atcgaaacat caccacgatc agcaatctct ctcgtgcaaa tatgcaagta cttttagata
     5581 ttcggattgt tcctgaagaa ggatatgaca aaatctatga gatcattgat cgagtaaatc
     5641 gtacattagc tgaaaaatac caagaagagc tccaaactga accttctatc tttggattag
     5701 tggatcttgg aaacagcaat tttgcgattc gtacggtctg ctacgcattg aacggaaaac
     5761 aatatgcctt aaaagaagag ttcttgagct cctatgtcaa agaattgaca gcttctggtt
     5821 tcacgattcc gaacagtcca attgctgtcg gaaaataatt tggacaaacg atgcaacgaa
     5881 tctcactaaa aagagcgtgg gacaaaagta aagaacactt ttgtc
//
LOCUS       DACECY010000111         5912 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.117, whole genome shotgun sequence.
ACCESSION   DACECY010000111 DACECY010000000
VERSION     DACECY010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.117"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(540..1349)
                     /locus_tag="IWO38_002665"
     CDS             complement(540..1349)
                     /locus_tag="IWO38_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0314684.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1349..2095)
                     /locus_tag="IWO38_002666"
     CDS             complement(1349..2095)
                     /locus_tag="IWO38_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0314685.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2113..2580)
                     /locus_tag="IWO38_002667"
     CDS             complement(2113..2580)
                     /locus_tag="IWO38_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0314686.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2580..2990)
                     /locus_tag="IWO38_002668"
     CDS             complement(2580..2990)
                     /locus_tag="IWO38_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0314687.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(3001..4014)
                     /locus_tag="IWO38_002669"
     CDS             complement(3001..4014)
                     /locus_tag="IWO38_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009141002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0314688.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4231..4965
                     /locus_tag="IWO38_002670"
     CDS             4231..4965
                     /locus_tag="IWO38_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0314689.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVRNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4949..5647
                     /locus_tag="IWO38_002671"
     CDS             4949..5647
                     /locus_tag="IWO38_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0314690.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSHS"
BASE COUNT         1913 a         1238 c          982 g         1779 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga accggcgcaa aacatcagct taaagcgaca
       61 aaaaccacga gcaggtccat tcatcctcaa acctcggtga tcttcgcggt ttaacaccat
      121 cagaagaaat gcacaaaaat aataaggaga gtctgagaca taaataaatt cccagacttt
      181 tagtgagtct aaatccgaat aaacggtggg tcgaaagcaa ctctagctgc gcgtcgatcg
      241 cagcaatctc taggtgctat gttcggataa atggtggtca aaagtcagat tttcggccat
      301 ttcgtaacag ccaaccagac aaaaagcgtc tgttttgtct tctactccaa ttgctcaaat
      361 cttagcgact tttggcttgg tcttactcta aacagtttgt aagcctaaac tttatttgct
      421 gtttgtttct aagatcaaag caaaactact ttttagttgg ttacttttac aagttctttt
      481 tactgttgaa taaatgtaaa tcttaagaac aaatatttac ttctataatt ccaagtttct
      541 taaaaaattc cgccaagtgt acctaaaaag cataaaacaa aaattcctag aataattgtg
      601 atcgttttat gccctttgga gtaaagccag acgcataaaa aggtaattcc taatggcagc
      661 aagcccggaa aaatttgatc aaaaatactt tgtaatgaag tttcagctcc accaaccgtg
      721 aattttagtg acgtatttaa agtaaccatc gtgcctatca tcgcgccaat taccgttaaa
      781 cctaaaatcg aagcaccatg agataatttg tcaatcatat ggttgtcttc tgtatcatca
      841 ataaatttag ttcctaaaac atagccatat tttgtcgcca accatttaaa aataaaatga
      901 atggcagtat acaaagtaaa ataaaggatc atgcctaaaa tattcccttg ttgagcaaaa
      961 gaaataccta tgccagccgc aataacccga aaagttcccc agaaaaatga atcgccaata
     1021 cctgataaag gtcccattaa tcctgttttg attttattaa ttgcgtctgt atcaaattca
     1081 ggatttttag cattttcttc ttccatggtg acggacaagc tcataacaaa aggcgcaata
     1141 tgcggtgtaa tgttaaatgt ttccgtatgg cgctgcaacg ctgtataata atcatcatcg
     1201 ttcggatata ttttacgtaa ggaacgttca ataccattca tttgtccata tgccatcatt
     1261 ttatcgaagg accaagagga ttgcataaaa aaggagcgcc aaaatatact tcttaattct
     1321 tttttcccaa taacactttc ttttaccatt ataaatcttc ctcctcttca aaagttagct
     1381 tttgaacagc cagtaacgct aagcaaagtc cgacgacagc cacaccgaca acatccattt
     1441 tcagatacat tacgccgaca aatcctaaga aaagataagg catcatcgct ttattcgcca
     1501 tcattttaat taataacgct agcccaacac aagaaagcac accggccgca acatcaaagc
     1561 tgtccaatac aacctgagga atcaacttga tcatcgcatt gattgtagac acaccaaaat
     1621 aagtagctac aaaaactaaa atggccgatg gaaccccaac gattaaaggt tgagccacat
     1681 aatgatattt ttgtgctttt ttgaaatcat gattgactaa tgcctcatcc attttgttgt
     1741 taaaatacgt aattggcatt gcataggcga agttacgcca catctgaaga aagatggaca
     1801 aaggtaaagt caacgcaacg gcagttccta ctccttgacc cgattgaatc gcaattgctg
     1861 tacctaaaac actggaagca taaacttcag ggggcgtcgc actccctacc ataattgagc
     1921 ccatgaacat ggattccaag gtggcaccaa taataattcc tgactctaaa tctcccaaaa
     1981 tcaaaccgac aaaaggcgct gtaaatagcg gtctacccat catcgatgca ccaaatgtgt
     2041 attcatcaat agacgccaaa aaagctacca gtgcgattaa tattgcttgt atcatattct
     2101 tcccctcctc ttttagtcat gcaaaggatt gatttttttg tccgttggta cagcctgaca
     2161 ttgaactgtt atcccgtttt cagtcatttt tttcaccaag tccagttctt gttcattcaa
     2221 gtaaacggaa ctagtaatca ttttccgatt ctcgtttttc tttaaatttc ctaaattcac
     2281 ttctttaatc tcaggtacgt gctgagtaat ttcgtaggca tcctccaccg tcccgacaat
     2341 taaaaatact tggtatttct tgattttttc tccatttaaa acagagatgg cctctgtcat
     2401 atccttcacc acaaattttg tccctgtagg aacagccatt tttagtgttt ttttgcgcag
     2461 attattggta gcgatttcat tacttgcaac gaaaatacaa tttgcaccta agttatttgt
     2521 ccaggcaaaa gcaacttgcc catgaacaaa acgttcatct actcgtaata atttaatcat
     2581 taaaaatcat cctcttctgt tttctctagc aactcgttca ccaaaactat cccttcattc
     2641 ccgctattca tagctttttg gattagtttt ttcgtgtcct gttcacctga taagcaaagc
     2701 tctaaaagca gcggcagatt tacaccgctg attaaataaa aattgtcttt accgatttgt
     2761 tcggagaaag cgttagacac gcttcctccc agtaaatcta ccgtcacaat taattcgtca
     2821 tcaggcgcta agtcggaaaa tagctgctct atttcctggt taatggagta gtcactatct
     2881 atatacgcag ttattgctaa aagtgatttt tgaggaccaa taataaattc taaagtatcc
     2941 tttaagcctt tagccatcat ggaatgcgtc gcaattacaa ttttcctcat agctcttacc
     3001 ttaatattcc acagtccgat aataacgacg aatatctagt gaatgatccc gcaccttttc
     3061 aaaatgagca ctcactcgtt ctaaggccgt tgccataacc attggcgcta catactgacg
     3121 catagcgctt ggtacacctg ctaattcgaa atccgcagtg tcaaaaaccg tcacaacatc
     3181 gctatattta tttacaaaac gctctacgcg atcgactagc ggacgcgttt gatcttcacc
     3241 ttttaataac acaaaactca tagatttatc agtcatttca atagtcccat ggaaaaattc
     3301 ggctgcatgg atcgacttgg tggcaatcca ttgcatttct tccagtacgc acatagcata
     3361 ttcataggca gccccataag tattcccagc tccaacaaac atatgaaatt ccgtattttt
     3421 atgtttttct gcaaattcaa gtgctttctg atcattttgt tgccgaacac ttagtaatac
     3481 ttccggcatt ttttgtaatg tttgaataaa ttcatcatat tcaggaaact caccgttatt
     3541 tttcatcagg cgtgcaccca aagcaaaaaa ctgtaactga agttcttcta ctaatgtatc
     3601 gttgtcagca tcattttcaa tcgcataatc cacgatttcc ccgagtggtg tagcagcatt
     3661 tcctacaaac gcaatcgtcg ttgcaccttg actattggca tacttaacaa catcaacggt
     3721 ttcttgagtc gtaccggtta atgaagatgt gattaacact gtatctttat taatcttttt
     3781 cggctgtgaa caaaccacct ctgcggctat ttcatgataa taatcaagct ctgaatgagt
     3841 gcttaacaga taagctagcg ggctgattgt ggcatatgtt ccaccagagc ctaccaaata
     3901 aagattttta aatcccttct cactgattgc atctgccacc tgattgattg tctcgatatt
     3961 ttttactgca ccatttaata ctgatagaaa acgttcttcg ctaaaaccta acatagttaa
     4021 aaacctccaa gtcaataatt tgaaaaatat atttgcaaat gccggcacat taacaaacca
     4081 cttaatcttt tttatatacc acctgttaac gtttttaatg taccacttat agaaaacgct
     4141 gtcaatagtt tttaaccact tttattttta tttgtaccaa aatccttttt tcatgtataa
     4201 taaacaaaaa tagactctgg agtgtaacgt atgaatactg aagaactaac gaacaaatta
     4261 atacaagaca ttgaacaaga aaaatataaa tacgcgcaag ctattccttc agaacgtgat
     4321 tttgttcaaa tttatgaaat gaatcgttca gccgtaaaaa aagccattca acaattagta
     4381 aataaagagt acctactaaa aatacatggc aaaggaactt ttgttaaaaa aaatatccat
     4441 gaacatttgc aaatcaaatt tcatggtttg tctgaattgt tggaacggga agggattcag
     4501 cctagcagct taattttagc ttcagaaata aaaccggctg gacacattgt cgggaaagct
     4561 ttacagctgg aaccaactga agaagtattt catttggttc gtttaagaag cggcgacggt
     4621 gagccaattt ctattgaaga cacttatatt ccattaaatc tcatacctga tcttcaagct
     4681 tttgatttcc aaattttttc attttattcc acattggctg cttatcatta tcaaattgaa
     4741 aatatctatc agacaatttc cagctcaaaa gtgcgcaata aagaagcccg tttgttaaaa
     4801 accagcttaa atcgtccggt aatcagtctt gctatcactg cggttacgac aaatgaacaa
     4861 gcggttgaat acactgaagt agtggttttg cctgaattcg ccgccatcta tactaaaaat
     4921 gttcatcaag gaaaggagat gaaaatctat gcccaaattg attaatctgt tggcttatga
     4981 cctgattgac aaaattattg cttatattca tgaacatcat ttaacccccg gaacaaaact
     5041 gccttcggaa cgggacctat cagaaatgtt taatgttaat cgaactacct taagaggcgc
     5101 gttacaaaaa ctggtggaca acggcacctt acgggcgcaa accaatagcg gctattattt
     5161 aactgcgcca aaattcgaaa aaaatgccca tcatttcttt acaccgatag aagattctct
     5221 gcttagtaag cagcggctac agctaaaggc aacggctctt tcaccgaaaa aagcagcagc
     5281 tctttgtccg gaactgaatg atttcaaaca gcagcaacca caagcttatt tagaatatat
     5341 tgatgcagaa gcaattagct tgatgttatt aatccctatg gaaacattaa agaaaagtaa
     5401 tttctcggac aatcctttcc aagattttaa aagtttggca tatacgcgtt ctcaagaaat
     5461 ttctttaata actgacaatg acgttgcggc ggacttgctg ggagttgaca aagaagaggt
     5521 tctgttattt gtacgagagg aaataagagc cggtaaggag ttggcggcga ttgctttagg
     5581 ttatactatt agtcgccgct gcaacataca ggttaatgtt gctacgacct tgcaatccca
     5641 tagttaggaa aaactattcg ctgtaagttc aaccgtttca atgcaagcga atcacaaatc
     5701 aaaaagtatt taactcgtgt ttattagaaa tagtcatgaa cttttagtct ccaagatcaa
     5761 accgacagcg ttcaactctg agaagcaaaa gtaaatgtac gatcaagtat gaggctggga
     5821 cagaagcgct aagcggttct gttgcaaagt tttaaatcta ctatcagata aggtagaata
     5881 atagaaaaag atagcaggag gaatgacgat ga
//
LOCUS       DACECY010000112         5751 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.119, whole genome shotgun sequence.
ACCESSION   DACECY010000112 DACECY010000000
VERSION     DACECY010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.119"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..433
                     /locus_tag="IWO38_002672"
     CDS             <1..433
                     /locus_tag="IWO38_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ0314691.1"
                     /translation="KDYWGESKGFIYPDSNMGGNWKATNPKKEQEAMKLKNTKSNNLL
                     YATCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMGNWKFVENNSGGQNISSVSYETALL
                     EYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEKVLKKIAL"
     gene            453..1013
                     /locus_tag="IWO38_002673"
     CDS             453..1013
                     /locus_tag="IWO38_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HAQ0314692.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGILSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADE
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            1836..2603
                     /locus_tag="IWO38_002674"
     CDS             1836..2603
                     /locus_tag="IWO38_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAQ0314693.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIREKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            2629..3171
                     /locus_tag="IWO38_002675"
     CDS             2629..3171
                     /locus_tag="IWO38_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314694.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFATYESGMSAYDETGFDGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGYSLDDKTRLRLFDIEGKQIGLNKFCKNGKEFATKDF
                     IKGYRLLLENYGINLEESVK"
     gene            3172..3738
                     /locus_tag="IWO38_002676"
     CDS             3172..3738
                     /locus_tag="IWO38_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314695.1"
                     /translation="MFQKRKDKFPNLEVKSQKVIDGEMIELFQKNYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            3753..4001
                     /locus_tag="IWO38_002677"
     CDS             3753..4001
                     /locus_tag="IWO38_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314696.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDDTENLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            4021..4260
                     /locus_tag="IWO38_002678"
     CDS             4021..4260
                     /locus_tag="IWO38_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314697.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEDLLRKTQRKGYPDKILKQIGYYM
                     PEERIEKEFCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            4631..4912
                     /locus_tag="IWO38_002679"
     CDS             4631..4912
                     /locus_tag="IWO38_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAQ0314698.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFDEA"
     gene            4902..5243
                     /locus_tag="IWO38_002680"
     CDS             4902..5243
                     /locus_tag="IWO38_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0314699.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIGEWEKAGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSASDTKKL
                     NKFIERLQRKV"
BASE COUNT         2170 a          767 c         1162 g         1652 t
ORIGIN      
        1 aaaagattat tggggtgagt caaaagggtt tatttatcca gattcaaaca tgggagggaa
       61 ctggaaagct acaaatccca aaaaagagca agaagccatg aaattaaaga atacaaaaag
      121 taataattta ttatatgcta cgtgtaaaca ttttcgacat gtacgagata cagaatttac
      181 gagttatcat ctttcaggaa tagtaattga cagtttcgta tatgaggcta tgggaaattg
      241 gaaatttgtt gaaaataaca gtgggggtca aaacattagt tctgtatctt atgagacagc
      301 tttattagaa tactataact cacataaagt tatgggcggt ttaaatttat actctcctgg
      361 aagcaatcaa tttgttaatt ctgatagtag tatcatatgt ttagaaaaag tattaaaaaa
      421 aatagcttta taaagtttta tggaggaaca acatgggaga cgaaaaaaag cgcttggaag
      481 ttcaattacg tgaagcatat ggacgagtaa cgtatactta caccactcat ttaaagcaag
      541 ccgataatat tatttctgac aataaaattt taaggtgtct tcaaattggt ttgtcagccc
      601 ttactactgg agggatactt agtaccatta ttactgataa tagtgagtta acctttgttg
      661 ctggattatg ttcaacaatt tctcttggat taaacttata ctttaagaac tttgatcttg
      721 ctgaagaagc taaaaatcat caaattgttg ccgatgaatt atggttgatc agagaagaat
      781 acgtaagctt actaacagat ttaaatcgtt taagtgttga agatattgtg aaaaaaagag
      841 atcaattaat gaacgaaact catgaagcat ataaaaatgc ccctaaaaca aatagtaaaa
      901 gctatgaatc agctcagaaa gcattaaaat ctgaagaaga gcaatttttt acagaagagg
      961 aattggataa gatgttacca gagcatctta gaaaaaagag ttctaaatat tgaactcttt
     1021 ttttatgctt aaaattctct tgtaattttt ctattcaatc aataaataat ctatatttaa
     1081 gaagaaatat gatgatagaa acatctccta aagccaccgc aagccgttct aagcatagct
     1141 tagcccgaca ttgctagata tgctcatctc accatgaacg tttcctaaag ccaccgcaag
     1201 ccgtcctagt cattgcctag tccgacatcg ctagattagt gcatctcgcc ataaaagttt
     1261 tctaaagcca ccgcaaaccg tcctagccat cgcctagtcc gattttgctt gatcggttcc
     1321 atttctgttt atttagtggg tcaacaccgt aatcttaaat agccatcgcc tatttaagat
     1381 tactaggtct gctttgaagg taaaaagtct ttcattcttc aagactttta ggctttcaag
     1441 ctacgcctga aagcattgaa attcatcgct ctttatttgg ttttgccatt tataattcct
     1501 tacgcccatt atctttttcg cctgtcttcc ttgtcagaag tgtttagagc ttatctcatc
     1561 tttggattaa tacaagtggc gtaaattttt ttctaaacgc aagcgttacg aaaataattt
     1621 acttaaacac ttgtattaat ccggcagata agaacgcatt ctaatcactc ccgacaaggc
     1681 aagacaggcg aaaaagataa ataaatgaac gaaaggaaga aacataaaat gcaaaaccaa
     1741 atagctatga agaaaaaaac acctccctct aaaacaatcc aaacaaaaaa gaattttgaa
     1801 aaaccaaaaa tcaaaaacag aaagaagcgt tcaacatgag agatagaatt ttagaagtta
     1861 atcaagaaat ggtaaacaag ttaacaaata tcagtgggaa ctatgaaaag tacgatggta
     1921 ctttacttga taactatatt tttcacgata cacaaaatat cagaattggc agaagtaaac
     1981 cgagagcgtt cattctcatc agagaaaaat atttgaatga atggaatagc acctatgaaa
     2041 tggttatgac tgataacttt gaaaaggtat tggatttcag aaatgctttg ttgggttctg
     2101 atatgggaaa tagcagtctt gaatctattg aagttgaaaa caagaccgag aatgaaggtg
     2161 aaggattgat taagaatgtc ttgaaaatcg aacaaaaata tatcagtgaa gatagaagtt
     2221 tttatagagt cacaagctcg gttggtctag caaactttgc tactaactta attggaaaca
     2281 atgctagtga atctttgcta gtgatgtgtc tgaatacaaa gaatgaggtc attgcttata
     2341 gcgaagtttt caaaggtgga attaatcaat cagtagcgat accaagagag atttttcaac
     2401 tagctttgct aaacaattca gcaaaaatcg cagtatcaca taatcatccg agcggatatg
     2461 aatttccaag tgaaaatgac aaaagtttta ctacgaattt gaaaaacgca ggaaaaacaa
     2521 ttggtattga attgttagac catatcatcg taacaagtaa aaacacaagc tattactcat
     2581 ttcaagaaaa tggttcttta taaaaattag agaaaagagg aaaacaagat gggcgaaaga
     2641 acacaagcgt taattacagt caaatatggt aacagcaaaa cgaaagcaag cgtgtcgatt
     2701 cattatcagt gggggtatgg tcgatatatg ctgatggacg tattgaatca aggaatttat
     2761 ttaatcaata aagcatacga cttacgagat aaatcaagag aagaattagc aaaaggaatc
     2821 tttgatagaa gtacaggggt tatcaatttt gccacctatg aatcaggtat gagtgcttat
     2881 gatgaaacag gatttgatgg gcgctatgaa ccttatcaag aaacgattga agatgtgatg
     2941 aagttttctg ataacaatga tggctatgct catttagaga tggtgttcga tgaaaatgga
     3001 tatagtctag atgataaaac cagattgcga ttgtttgata ttgagggtaa acagatagga
     3061 ctaaacaagt tttgtaagaa tggaaaagag tttgctacaa aagattttat taagggatac
     3121 agattgttgc tagaaaatta tggaataaac ctagaagaga gcgtgaaata aatgtttcaa
     3181 aaaagaaaag acaaattccc taatttagaa gtgaaaagtc aaaaagtgat tgatggtgaa
     3241 atgattgaac tattccaaaa aaattatgga acgatagaaa ctaccgcaag ttgcaaatac
     3301 tactttcaag gaaagagaaa cacgtttaaa ggctttgccg attctgatga agaattggca
     3361 aagaaaatca ttgattggct tgtcagcagt gaacacgtag caagagaaga taccgttaga
     3421 acaacgatta atggggaagt cctaacaggt actagatgtg tttgttgtaa tcatgaattt
     3481 gtagaagatg aaggttgcta ccgaatcaat gaatatggtg attttatttg ccagagttgc
     3541 cacgagatag aatttccaac cgaaagaagt tggtttgaat acatgctaca agattatgag
     3601 atggaaaaag aaaatggtga agtagttttt tgtaccaaag aggaattgag taagagaagc
     3661 gatgatgaaa ttgaagattg gtgtgtgtgg ttagtagggg cagatgattg cgttgcatat
     3721 tacacaacag cagaatagga gtgtgaatca gcatggaagg aatggatttt ctagatcatg
     3781 aagatttggt tgattttggt tatacatgga aaggaatggt aggtattagc agaagtcttg
     3841 ctaatgcctt ttacgaaaga aattatgcag tatatgttct ttatgatgac gatacagaaa
     3901 accttgttga cgaggaatat aaattggatt tagagaatgt tttatatgga atagaaaagg
     3961 aagatttagc aaaatatatt ttttcatggt tgggtcaata agtgaaagga agatagaaac
     4021 atgacaaaat ttaaaagtaa gggtatgcaa tggtgttctt attattatgt tggaaatgat
     4081 gaagatttat tgagaaaaac ccaacgaaaa ggttatccag ataagatatt gaaacaaata
     4141 ggttattaca tgccagaaga gaggatagaa aaagaatttt gtgaggttct ttttgatagt
     4201 gaacagctat tagttttgtt aacccaaaat aaattgattt gccaatttga aaataattaa
     4261 gatgaggtgg tgaaccatga atgaagataa gatggtatta agtgaatatg ctagattgag
     4321 aactattaat tctttagcat ctcatttaag ttgttctttc cttacaaagg tgctaaaatt
     4381 aatttacaaa tgtgaagatt ttgtacaaga gaagcacaaa gacaagccta ctgcgaatag
     4441 gcttgtcttt ttttcattca ttacgattcc gtatgtgtgg gttagcgaca atttgttcaa
     4501 ttaccgtatg atatagcatc ttaatttgat tagattactt gcaatgtagt ttattaagag
     4561 tataatataa tcataaatgt ggataatttg agaaataatg agtgttttat actcaaaagg
     4621 agtggatgaa atgaaggtca aaaatccaac tgaaaaaagc agaatccaag tcggaataga
     4681 caagaattta aaagagaacg cagaaatgat attggaagag ctaggtttaa atcctacaac
     4741 agcaataaca attctttaca aacaagtagt cgctagaggt gagtttccag tagagataaa
     4801 gctaagtgaa gaggagaagc aaggaatcag attgcaacag ctaactaagg acatgccagt
     4861 agatgttcta gatacagatg aaaagttaga agagtggttc gatgaagcct aatgagatag
     4921 taacggtcta tgttgcattt acagataaaa atggtggtaa aagaagacct attttggtgg
     4981 ttagtgataa agaagataga gtagaatttt ttggaatcac aagtcaatat gagaagaaat
     5041 cagaccaaat aaagagagtg tattttccta ttggagaatg ggaaaaagca ggtcttaaaa
     5101 agcaatcatg gattgacgta gggtctttaa aggctattcc caaaagccga gagaacatat
     5161 catttaaaaa ggttggtatg ctatcagcta gtgatacgaa aaagctaaat aagtttatcg
     5221 agagattaca aagaaaagtc taacaaggct tttctttttt tgcttatatg tatattaaga
     5281 tatacgtcta tacaaacgta tttatgtata taaaaataca cgtaaatata tacgtgaata
     5341 taaacattaa gaaaatatta agaatgatta aatagtggta attgttaata tacgaattat
     5401 aagatatatt ttaaatcgaa tgtatgtaaa taaataaaat gactggagga tatttaatag
     5461 tgataaataa cagtaatcca gacggataca gtgagtgaag tagcttgcta cgaacgaaca
     5521 agcagaatga aacggtagtt tcattagagc aagtcgcttg cgacgtagcg ttaccgctga
     5581 tgtaactggc aagttacata gagagagttt gcttgcaaac gaacgttacc tacttgaata
     5641 gaaaacaaaa ataacagatt agtgaatttt aaataggagc cacaactcaa ccaaccgctt
     5701 gcggtgggtg agtcccacgg aggaaaagac gaacgaacgg tcgcgtagcg a
//
LOCUS       DACECY010000113         5466 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.120, whole genome shotgun sequence.
ACCESSION   DACECY010000113 DACECY010000000
VERSION     DACECY010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.120"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            133..1107
                     /locus_tag="IWO38_002681"
     CDS             133..1107
                     /locus_tag="IWO38_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0314700.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1089..1550
                     /locus_tag="IWO38_002682"
     CDS             1089..1550
                     /locus_tag="IWO38_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0314701.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1600..2064
                     /locus_tag="IWO38_002683"
     CDS             1600..2064
                     /locus_tag="IWO38_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0314702.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2078..2827
                     /locus_tag="IWO38_002684"
     CDS             2078..2827
                     /locus_tag="IWO38_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0314703.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2827..3657
                     /locus_tag="IWO38_002685"
     CDS             2827..3657
                     /locus_tag="IWO38_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0314704.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3657..4304
                     /locus_tag="IWO38_002686"
     CDS             3657..4304
                     /locus_tag="IWO38_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0314705.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4402..5178)
                     /locus_tag="IWO38_002687"
     CDS             complement(4402..5178)
                     /locus_tag="IWO38_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0314706.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5260..5403
                     /locus_tag="IWO38_002688"
                     /pseudo
     CDS             5260..5403
                     /locus_tag="IWO38_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
BASE COUNT         1872 a          739 c          973 g         1882 t
ORIGIN      
        1 attttattat ataaaattgt tataaaaaat tttttttgaa aaatagtatt gcaaaataaa
       61 gcaatttcat agataatagg tatcgtaaag ttatataact ttataatacc tattagagtg
      121 agggataaag aaatgagaca actacacaaa gaacagattg aaacagcaat ggcagcgtta
      181 aaggaaaaga caattgatca tgtatatttt acagcatgtg ggggttcaca agccgtttta
      241 atggctggtc aatatctatt tgataaagaa ttaactgtac cttgtcatgt ttatactgct
      301 aacgaattta actatgctat accaaaagga ttatctgaaa attctttagt tatcacttgt
      361 tcccattctg gcacaacacc agaaactgta gaagcaacaa aaaaagcaag agaatttgga
      421 gcaatgacta tttgtttatc taatgaagaa ggatctcctc tatgggaagc agctgaatat
      481 cctgttcact atgattgggg taaagatgtt gatgctagtg atttaaataa aggaattctt
      541 tatggtttat tatttaatat tttacttgtt ttagatggct ctaaaaaatg ggaaacttgc
      601 ttgaatgaat ttgataagtt aaatgatctt gttattgctg aaaagagcaa atttgaaata
      661 actgctaaag aatggggtaa aaaccaaaaa cgagataaaa ttatttacac aattggtagc
      721 ggtattaatt acggagaaat ttactccaca gcagcttgct ggttcatgga aatgcaatgg
      781 attaattcta atgctattca ttctggggaa tttttccatg gtccttttga aatcactgat
      841 tatgatgtac cattcatctt agtgaaaagt attggagata caagattttt agatcaacgg
      901 gtagaagact tcactaaaaa attcagtaat aaattaatgg ttttagacca agctgatttt
      961 gacttagaat ctatagatag tgtagcgaaa gaatatattg cagctgtact tagtggcgtt
     1021 gttattcgcc aaatggtaga agcgattgct tttgaaagag gccattcctt agatgttaga
     1081 cgctatatgt ggcaaatgaa atattaatta ataggaggtt gcaaaagatg gcaaatcaat
     1141 tatttcacat tgtgattaca acacattcta ccctttgtga gggttattta aaggcaacaa
     1201 aattaatttt aatgaatgac gccacaggta ttagtgctgt tccatttaaa gaaaatacta
     1261 gtccagatga ttttgaggag agtatgaaag aaataattag tcagcatcat ttgcaacctc
     1321 taattatttt aacggattta attggaggaa cacctaataa tgtttctatt aaacagttga
     1381 acaacaatag aattcaaatt attgctggga ttaatttacc tcttttactg gagttattaa
     1441 tggcccagtc agctggtaaa tctatagaag aaatagattt taaggaatta ctgaataact
     1501 ctcaaaaaag tatggttcat attaattcgc ttttaaaaga tgtcagttaa ggtggcaatt
     1561 tctattctat aaattttaaa aattgaagga ggaaatagaa tgattaaatt agtgcgtgta
     1621 gaccacagat tacttcatgg acaagtaata ttttcttgga ctaaacagta tgatattaac
     1681 catattattg tggctgataa caaagttcca ggagatccaa tgtctgtgat ggctttgaca
     1741 attgctaaac ctgctgattg caaattagac attgttaaaa ttagtgatgt taaagggatt
     1801 atggaaacat ccccaaatga taattacatg attttaatta aaggacctaa ggaagctttt
     1861 gaattagttc aaaatttacc agaaattaaa gaaattaatt ttggcggtgt gtctaagaaa
     1921 agcgagtcca aacaatatgg aaaggcagtc tttttaactc ctgaagaatt agaagccaca
     1981 aaaaaaattc ttgatcaaga agtgaaaata tttgtgcaac aagtcccatc ttcaagtatt
     2041 gaaaatgtag attttagtaa gtaagggggg aaaataaatg ttattaaaag ccattttatt
     2101 aggtttagtt ggtgtggtag ctgttattga ctccaggtta attggtcgac aaaatattgg
     2161 tagaccacta attttgagta cacttgccgg acttgtgcta ggtgacttgc atacagggat
     2221 tgttcttggg gcatctttag aattaatttc tatgggattc gtctcaattg gggcggctgg
     2281 acctcctaat atgcaacttg gtagtattat tgccactgct tttgcaattt taactaatag
     2341 tagtacagaa acagccttaa caatagctat cccagtggct gtagctggtg aatttatgag
     2401 tattatcatg cgtatgataa ttgctcaatt tggacatgct gcagataaag ccattgaaaa
     2461 taataaattt agaaaagctc aaattattca tatttattgg tcatttattt ttaatgcttt
     2521 tgtttacttt attccaatct ttttaacagt atattttggt gctgacgttg tggcagattt
     2581 agtagataaa attccagaaa tgattacaga cgccttgact gtctctggaa atatgctttc
     2641 cgcattaggg tttgcaatgc tattaagcac aatgttgagt aaaaaattat atccttactt
     2701 tatttttgga ttctttattg ttgcctattc aggtctttca ctaattggcg tgacaatttt
     2761 tgcagctctg attgcctttg taatggatca agttaaatat ggaaaaagag aagaggcgca
     2821 tggttaatgg aaaatagtac agaaaaaaaa cgtttaatca caaaaaaaga tcttcataag
     2881 atgtgtcttc gttcatggac aatcatgtgt tcgtggaatt atgaaagaca aatgcatatg
     2941 ggatttatgt atgggatggc gccaatttta aataaagttt ataaaaatga tgaagagaaa
     3001 aaaagagaag cttatactcg tcatatggca ttttttaact gtacgcctca aatgactcca
     3061 tttattatgg gactttgtgc ttctatggaa gaacaaaatg ctcaagcaga agaaggaaac
     3121 tttaatactg aatctatctc catgattaaa accagtttaa tgggtccttt tgctggaatt
     3181 ggtgattcat ttttccaagg tactttaaga attattacat ttgggattgg attgtctttt
     3241 gctaagcaag gtagtatttt aggaccgatt ttagctgttg gcctttttgc tataccttct
     3301 attttagctg ttttttacgg cggtaaatta ggatatgagt ctggcaataa ataccttact
     3361 aaattgtatc aagaaggtct tatggatcga gtaatgcaat ttgcttctat cattggtttg
     3421 gctgtagtag gtggaatggt ggctagcatg gtgccaattg tcaccccttt aaaattcact
     3481 acagggggaa cagaacttgt tttacaagac ttacttgatt ccattattcc tggattatta
     3541 ccacttgtat gtactttggg aatatataaa ttgattaaga aaaatgttag tacaaatatg
     3601 ctcttattag gttgtatttt ggcagggttt gttttaagta taataggtat cttataatga
     3661 ttaaggctct aatttttgat atggacggag ttttaattga tagcgagatt atgtatcaaa
     3721 agtgggtatt taatttttta aaacaagaaa atttaagcta tcctttgtat tcttatcaaa
     3781 aaaagattgg aactacagca agtatttttg atgatataga aaagtacaat gagggatgta
     3841 acagtttaga attgaaggga aaatttacta aatattggaa atcgcaaaag attgattact
     3901 tggatatttt tcctaatata ctaattgaac agcttgaatg gttaaaaaag aaaaaaataa
     3961 aaatggcaat tgcttcttcc agtccaatgg atactataaa tgaagttgtt accacttgta
     4021 aaatagagtc atattttgat tgtttaatta gcggaagaga tttgccagaa agtaaaccta
     4081 atcctactat attccttaaa gcttcagaac agctcagaat tccagttgaa gaatgtctgg
     4141 tgatagaaga ttcttataat ggtataaaag ctggtaaaag agcaaatatg aaggttcttg
     4201 caattaaaga taaacgtttt tctcaagatg taagtctagc agatgatgtt atttacgata
     4261 ttaaatatct gcgacaagaa gtacaggtga aatttggaat ataaataagt tttttgatgt
     4321 atttagagaa tattagtaaa aataaaaatc atgcccaaat ctaatcaaaa ttgattgctt
     4381 tgggtatgat ttcttttaat atcaatctat tactttagga tctgaatcat ttttatgcac
     4441 cgtatctaaa gtgatatttt ctgtgacttc aataacataa ctaattctat cacaacgaac
     4501 tgtggattga gaataatgaa atggttgatt atttttatct ttgccaacct tttctaaaat
     4561 aaatagcgga tctcctacta tacattgcaa taagtcggaa agctcaggtg ttgcagataa
     4621 accgtttatt tccataatag agctcgtagc tttgacatca tagtcatctt ctagaagttt
     4681 atataatgaa gtatattggc tacctttttc aaataaagtt ggaaattttt cttctgatgc
     4741 gaaaaaatta tcaattccca tcggggcacc gtcttcccac attaaacgtt taatattgta
     4801 aactctatca gttacattga tttgtaattt attagctaaa aactcatcag cctttataat
     4861 tttagaatgt aaaacagtat atctaatttt atgtcctcgg gtagaaagag aatcgctaaa
     4921 tccatgaact ccagctaatt ttaaactttc ttttttttga gttacaaagg tccctttccc
     4981 ccttaatcgt tcaactgttc cactttctgt taaaagttta aaagcttttc gaatggtaat
     5041 cttacttact ccataaattt tacataattc ttcttctgta ggtaattgct ctcctggaag
     5101 atattttttt tcacgaatat cgttacgtat agaagcttct acttgtagat ataatggtct
     5161 attatcttga aaattcatag tacactccct aaaaagttat acttcattat atcacaattt
     5221 attaaaataa aagattaaga tataaatata aatgaactag tctttgacta accaatgccc
     5281 gcttttgaaa gaaaacaaat tcattctgtt ttaagtaaaa atccttatgt taccactcat
     5341 tctgaagggg atgagcctta tcgctacttg gttgtggaac caagcgataa gacaatttta
     5401 taaattcaat attaattaaa ataaaaacaa acaaagtaga ttattttact ttgtttgttt
     5461 ttttat
//
LOCUS       DACECY010000114         5388 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.121, whole genome shotgun sequence.
ACCESSION   DACECY010000114 DACECY010000000
VERSION     DACECY010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.121"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            175..792
                     /locus_tag="IWO38_002689"
     CDS             175..792
                     /locus_tag="IWO38_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0314707.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            833..1132
                     /locus_tag="IWO38_002690"
     CDS             833..1132
                     /locus_tag="IWO38_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314708.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1166..1333
                     /locus_tag="IWO38_002691"
     CDS             1166..1333
                     /locus_tag="IWO38_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0314709.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1378..1983)
                     /locus_tag="IWO38_002692"
     CDS             complement(1378..1983)
                     /locus_tag="IWO38_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0314710.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            2108..5095
                     /locus_tag="IWO38_002693"
     CDS             2108..5095
                     /locus_tag="IWO38_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAQ0314711.1"
                     /translation="MDEIKSNKKAFGIYLGEPSTEQLNQFFYLHEKDLELMETIRRPA
                     TRLGFALQLVSLRFLGTFILDLTNIPQNVLSYVATQLNIDPGEIIYYSRRQTRYQHTK
                     LIKSHYGYQDFNQPEIETYLANWLLNRALYTTESSDMLFDMLLKKCLDEKIVLPGITT
                     FSRFVASAIEKAEEHLYRQLSLVPTEDERKRLLNHLELIGTPVYGAIIKMDILRTPLA
                     DDSHKEIARGFDRLKQFQAFSTEKWKMESIPEGKIKVLANYAFKAKAQLIQRMSEQKK
                     LALLVAFIFIYKRKAMDEQILALSNFFETVFRRAKNKESKERLRTIKDLDRAASTLSD
                     IVDLVMSSHSDTNELSLRDRILEKYSEENVTEAVSQVRLLVKNDREPIAISELLNTYR
                     KFRKFIPDILTTLTFDSNDYGNNCKELWTFINNRFPKPITYKMFGAIKHTLPKKWIYY
                     IHEYPEQTNKCVLIAAVELLIQSLKRHDIYIPQSVLYNNPMDCLIDEKTWVQQKYVLL
                     EQLDLPSSANEMSLWLKNDLELSYKEALNRWPTSKMARIENADRLVVSRLKKTYEKKE
                     DKSFRRRIQQLLPKIDLSDLLLEVNQQLNLTQSFNHLSDKDTKMKDLDISLLAVLLSE
                     SCNIGFSPVSKEGFDSLKYDRLVYVNHHYIRVDTLSGANQKIIHAHRKLATSKIWGDG
                     HMASADGIRYITPQRSLYSRSNPKYFGRGRGITFYNFVSDQYIGFHGMVVSGTLRDSL
                     YLLEGFLNQPSELTPNQIMTDTAGYSDMIFGLFGLLGFQFSPRIANDKGTKLWRIDAN
                     AHYGELDTLSQNSINMELIYTHWEDILRVAGSLKSGKVNATELTKSLQRNGQPTPLGK
                     AITEYGKVYKTKHQLRYISDEIYARQILEQLNKGEARHALCRNIFYGRKGKLYQTYYD
                     GMEEQLNALGLVTNAIIYWNSLYLEKVFEQMKAEGFDCSEEMIRRQSPLISDHINFVG
                     KYTFRYEAELSNGKLRPLNVEEF"
BASE COUNT         1812 a          906 c          991 g         1679 t
ORIGIN      
        1 aaatataatc atgcgggagt gtaaacttac tgtttcaaaa agtatacttt ttgacatgcc
       61 ttgattttct ttgtttatgt atggcataat aaccacataa tacaatttga aacgtttctg
      121 atttaatctg attgttttga aacaatttta gtgaaaaaag ggtagataaa aacgatgaga
      181 aaattttttt atatccgggt cagtagttat gatcaatcga tcgaacgaca actgatttcc
      241 gcgaaggaat taggaatccc tgaagaatat atatttgtag aaaaagccag cggcaaagat
      301 tttaaacgcc ctgaatatca attaatgaaa cggatgtttc gaaaaggaga tttactctat
      361 ctacaatcat tagatagact aggcagaaat aaacaaatga ttttagaaga atggcgagaa
      421 ttaactaagg aaaagcagat tgatatttct gtgttagaca tgcctctgtt agatactgct
      481 agttacaagc aactagatgg gttagagacg cttatctctg acttaattct gcaattactc
      541 agctatatgg ccgaagacga gcggaaacga attaatagcc ggcaaagaga aggcgtagaa
      601 gctgcgaaac aaaagggtgt aaaattcggc agaaagaaaa ttgctttacc tcctgaattt
      661 cctgcgatct attctgactg gaaagcagga aagataactg cagtctatgc tatggatcaa
      721 ttgggcatga aacgaaatac cttttatcga cgggtgaaag aatatgaaca taccttgcac
      781 cacttttctt gatagatata acagctatta gacgggaagg gagagtttga aaatggactt
      841 aacgaatcaa agggtcgttt ttactggaac gctgcagcaa atgacacgaa cccaagccat
      901 cggtttggtt cactctttaa acggccattg tcaatcaagt gttacttcaa aaacaaattt
      961 cctggtttgt ggtcagtatt ctaagtcgtt gtttgatgat tctctctata caaaaaagga
     1021 acaaaccgcg cgggacttga ttgcgttagg tcatgagatt accattttat cagaagtcga
     1081 attttatgcg ttaattagtc aacagttgaa agaatggcaa cggtatttat aatcgttagt
     1141 aaaatttaca aggaggaaaa tttttatgaa gcgaaaatct tatgatcgtt ggggcattga
     1201 aataactagt tttaatcgaa atattgcttt tcacgtggag tgtgactgtg gggaattagc
     1261 aaaaagtgtt tatcaaaaaa attactttta ctgtcctaaa tgccatcgcc gatacggggt
     1321 acatggaaca tagaaacgaa atagtgcggt agaaaagcga atgaatgtag tatatttcta
     1381 caaatcattc gctttcttta ttttataaat agttggtcga gagatcccgt tttcttttgc
     1441 aatctctgca actggaatgc cttgctttaa catgtccacc acggttttaa aaactaagcg
     1501 tttttgcgga tctgttgagt taatagaata ctcgattggt cgtcccttat atcggccggc
     1561 tttttgagcc aataatactc cttgccgctg acgttcacga attttctcac gctcgttttg
     1621 tgcaatataa cttagcagtg ataaaaacat atcgaacata gcggtatcca ggagttcatt
     1681 tccagtatta aaatctaaaa actgtgcatc taatattttt aacgctactt ttttctgttt
     1741 cataaaagca actgtatttt ttatatcttc ataatctctt cctaaacgat ctagtgatgt
     1801 aacgatgacg caatcacctt cacggatata atttagtaat ttttgaaact caggacgctc
     1861 agtatcttta ccactggctt tttcttggaa taatttatca atgttaagtc cttccatcat
     1921 tgccaattgt cgatccaaat tttgatcttg tgaagatact cgaatatagc caatttttcc
     1981 cataaaaaac tcctttttaa aagtgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgctatcat agcattttta aaaataaatt ttatgtagac ttaaaattta cttcatggag
     2101 ggtttaaatg gatgaaatca aaagtaacaa aaaagcattt ggcatttatt taggagaacc
     2161 tagtacagaa caattaaacc aattttttta tttgcatgaa aaagatttag aattgatgga
     2221 gactatacga cgaccagcta ctagattagg ttttgcacta cagttagtct cactacgttt
     2281 tttaggaact tttatattag atcttaccaa tattccccaa aatgtattga gttatgtagc
     2341 aactcaacta aatattgatc ctggagaaat tatctactat agcaggagac agaccaggta
     2401 tcagcacaca aaattgataa aatcacatta tggatatcaa gatttcaacc aaccggaaat
     2461 agaaacatac ctagctaact ggcttttaaa tagagcgctg tatacaacag aatcttcaga
     2521 tatgttgttt gatatgcttc taaaaaaatg tctggatgaa aagattgttt tacctggaat
     2581 tactactttt tctcgttttg ttgctagtgc aattgaaaaa gctgaagagc atttataccg
     2641 acaattgtct ttggttccaa ctgaagatga gagaaagcgg cttctcaatc atttagaatt
     2701 aattggaact ccagtttatg gggcaataat caaaatggac attttgagaa ccccgttagc
     2761 tgacgatagt cataaagaaa tagccagagg ttttgaccga ctaaagcaat ttcaagcttt
     2821 ctctactgaa aaatggaaga tggaatctat tcctgaaggg aaaataaaag ttcttgcaaa
     2881 ttatgcattt aaagctaaag ctcaattaat tcaacgaatg agtgaacaga aaaaactggc
     2941 gttgttagtg gcatttattt tcatatacaa acgtaaggct atggatgaac aaattttagc
     3001 actatctaat ttttttgaaa ctgtttttag acgcgccaaa aataaggaat ctaaagaacg
     3061 tcttcgtacc ataaaagatc tagatcgagc cgcatcgaca ctttcagata ttgtagattt
     3121 agtaatgtcc agtcattctg atactaatga actatccctt cgcgatcgta tcttagaaaa
     3181 atattctgag gaaaatgtta ccgaagctgt atcgcaagtt cggctattgg ttaaaaatga
     3241 tcgtgaacca atagccattt ctgaacttct gaacacttat cgaaagttca gaaaatttat
     3301 tccggatatt ttaactacac taacatttga cagcaatgac tacggtaaca actgtaagga
     3361 actctggacc ttcattaata atagatttcc taagccgatt acttacaaaa tgtttggagc
     3421 tattaaacat actcttccaa agaaatggat ttattacatt catgaatacc ctgaacaaac
     3481 taataaatgt gtattgattg cagctgtcga attgttaatt caatctttaa aacgccatga
     3541 tatttatatt cctcagagtg tcttatataa caatcctatg gattgcttga ttgatgagaa
     3601 aacttgggtt caacaaaaat acgttttatt agaacaacta gatcttcctt cttcagcaaa
     3661 tgaaatgtct ttatggttaa agaacgattt ggaactgtct tataaagaag cgctaaatcg
     3721 ttggcctact tctaaaatgg ccagaataga aaatgcagat cgcctggttg tctctagact
     3781 taaaaagaca tatgaaaaaa aggaagataa gtcttttaga cgtcgcattc agcagttact
     3841 tcctaaaatt gatttgtcag atttgttatt ggaagtaaac caacagttaa atcttactca
     3901 atcatttaac cacttaagtg acaaggacac caaaatgaaa gacttggata ttagtttatt
     3961 agcagttctt ttatcagagt cctgcaatat tggtttttcc ccggtttcaa aagaaggatt
     4021 tgattcttta aagtatgatc gtcttgtcta tgtcaatcat cattatattc gagtggatac
     4081 actttcagga gctaatcaaa aaataataca tgcacacagg aaacttgcca cctctaagat
     4141 atggggtgat gggcatatgg cttcagctga cggtattcgt tatatcacac cacaacgttc
     4201 cctatattct agaagtaatc caaaatattt tggccgtggt cgtgggatca cattctacaa
     4261 ttttgtctct gatcaatata tagggttcca cggaatggtc gtttcaggga cactgcgaga
     4321 ttcgctttac cttctagaag gattcctcaa tcaacccagc gaattaactc ctaatcagat
     4381 catgactgat acagctggtt atagcgatat gatttttggt ctctttggct tattagggtt
     4441 tcaatttagt ccaagaattg ccaatgataa aggaaccaaa ctttggcgaa tcgacgctaa
     4501 cgctcactac ggggaacttg atacgttgtc tcaaaattca attaatatgg aactgattta
     4561 tacccattgg gaggacattc ttagggttgc aggttcgttg aaatctggaa aagtgaatgc
     4621 aacagaatta acgaaatctc ttcaacggaa tgggcaaccc acgcctttag gaaaagcaat
     4681 aactgagtat ggaaaagttt ataagacgaa acatcagctt cgttatattt ctgacgaaat
     4741 atatgcacgg caaattttag aacaattgaa taagggggaa gctcggcatg ccttatgccg
     4801 taatattttt tatggacgaa aaggaaaact ctaccagact tattatgatg ggatggaaga
     4861 gcagctcaat gcattaggat tagttactaa tgccatcata tattggaact cactgtattt
     4921 agaaaaagta tttgaacaaa tgaaagctga aggattcgac tgttccgaag aaatgatacg
     4981 caggcaatct ccactaatta gtgatcacat caattttgtt ggtaagtata cgtttagata
     5041 cgaagctgaa ttatccaacg gtaagttacg tccattgaat gtagaagaat tctagcaatg
     5101 ttcgggtttt gcatgctacc gcactatttc gtatgactgt tccagcgaat cacttcgaat
     5161 agaaaaagag aaaaacacga gaacagcgtt aaatcaacgt ttcttgtgtt tttacttttg
     5221 tgctaccgca ctatttcgtt tctatgttcc atgtacccca aaatcggtaa aagagaaggc
     5281 aatcaagaga actttattac gaatatcaaa aaattcattg ttcatttact ggaagctgta
     5341 cctaacgata tagaaaaact aaatttttct gattaccagg aacagaaa
//
LOCUS       DACECY010000115         5060 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.122, whole genome shotgun sequence.
ACCESSION   DACECY010000115 DACECY010000000
VERSION     DACECY010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.122"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(20..982)
                     /locus_tag="IWO38_002694"
     CDS             complement(20..982)
                     /locus_tag="IWO38_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0314712.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(984..2423)
                     /locus_tag="IWO38_002695"
     CDS             complement(984..2423)
                     /locus_tag="IWO38_002695"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ0314713.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2608..4554
                     /locus_tag="IWO38_002696"
     CDS             2608..4554
                     /locus_tag="IWO38_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAQ0314714.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4818..>5060
                     /locus_tag="IWO38_002697"
     CDS             4818..>5060
                     /locus_tag="IWO38_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0314715.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         1630 a          856 c          840 g         1734 t
ORIGIN      
        1 ttaaaaaatt aatttaacgt taaatacttc ctccagaaag tattgagatt ggtaaaatat
       61 aatccttatt tgtcttttcg cctttaattt ttttcagaag aatatcaaca ataagacttg
      121 caatttcatc aattggttgt cttattgttg tcaattgtgg gaaaaatttc tcaataaaag
      181 aggtaccatc gtatccaata atttttaaat cttctgggat attaagttta agttgatgag
      241 caattttcat agtgagaatg gctgttaaat catcagaaac aaaaatacca tctggtttgt
      301 gatttataat agttgacttt atttccattt cacgacggat agtagacaga ttattaggaa
      361 ttttgaaaat ttttccattt ggattttgaa aagaaaaacc tagtgcacgt aattctgtgg
      421 gagaatccgt gttatcattt cctgtaatca taatggtatt ttgacaacca tttttttgta
      481 aagtcttagc tgcaagtttt cccccttcaa agttatctga agaaacaatg gaaacattag
      541 gagctaaatt tctatcaaaa gcaacaatag gtgctctcac acgctcataa tcagcaattc
      601 caagattatg gcttgatgag attatgccat caacttggtt ggcttcaagc atttcaatgt
      661 attctctctc tttagcagga tcattttgac tattgcaaat aatcgtttta tatccatgtt
      721 taaataactc tatttcgaga tactcaatga gttcagagta aaagatatta ctaatattag
      781 gaaaaataag accaataagc tgaggtgact ttccttgaag tccacgtgct aaattatttg
      841 gtttataacc aagagttttc atagcttcat gaactttttg ttttgttttt tctgataaat
      901 atcccttatt attaatgacg cgtgaaacgg ttgttgggct aactccagct aattcagcga
      961 catcagttaa ttttgctacc attttaatat tttaattcaa agtattttcc agtaggttcg
     1021 cctgatttga tttgaatacc attttggtga ttttcaggga atacacgact agtaaacact
     1081 ttttctcctt tattaataaa aatctcaatg attgagttat ctacaaaaat gttagcagtt
     1141 gtttctttag tatcaattga gcattcacga atcgtaccaa agtcagtggc atattgaatg
     1201 cctgctttgc tgcgatctaa aataattttt ccttctttgg tatctacaat caagctaagt
     1261 ccatttcctt tactatcaga gaaaagaagt aactccgatt tctgatttgg aggaaaagtt
     1321 agttcaagct cataagtatt atttgtttct gcttttgctg taaattgttc agaactagca
     1381 cgtaatgatg taatagcttc tactggatat tggtaaagtt taccatcttt tattgaaagt
     1441 tcttttacaa gactcattgc tccctgataa tcatacttat cagttggata atcgatatca
     1501 ggtagtccaa tccaactgac aatcagagca cgaccatcag gagtattgaa tccttgagtc
     1561 gcatacgctt caaatccata atcaagattt tgaatttctg aagcgcccac aagttttact
     1621 ttttctatgt caagcgattg acagatctta aaagtattcg gatagatatt cccataattt
     1681 agctcagatt tatccaaccc ttgaggacaa taaatgagaa caggtttttt gtctacaaat
     1741 actagatttg gacactcaat catgtattct gaaccagtgc cgccaaaatc aaggttgcca
     1801 acttcctccc atgtttccac gttattgtca tccgctttgt aaagtttgat aaagcctgat
     1861 ttttctaaat tttgtgctcc aacaatcgca tacaattgtc cttcataatt aaacatttgt
     1921 ggatcacgaa aatgatcagt tacatcagca ggtttatgaa taagaacatt ctcacctttc
     1981 gtaatgttgt agtctttatc catccatgct ccgatttgaa gtgtatcacg ttcccaattt
     2041 tcatttcgaa cgtttcctgt atagagtaag aatagattcc cctctatttc atatgcagaa
     2101 ccggaataag cgccgtggct atctcgtttt gtgtctggaa gaagttctgc tcctgttaca
     2161 tgaaaatgaa ctaaatcatc tgattcattg tgtacccatt ctttcaaccc atgagcagct
     2221 ccaaatggcc aattttgata aaaaagcgta tattttccgt taaaaaaaga aaaaccatta
     2281 ggatcgttta acaatcctgt tttaggttcg atatgatagc tagcacgcca aggtgatcgt
     2341 tttacattat ccttaatttt atccatctca tctttagtcc aatcagaata agcacgatat
     2401 cgtatttctc gaggtaagtt catattttac tccttcaatt tatcttttta caactagtat
     2461 ggtaaacgtt ttccgttgaa aaatcaatca tttcatttaa aaaaataaac tatatgacaa
     2521 acgtttgaca tatttttaag atatgctaaa ataaatttgc atttcagtga accgttttca
     2581 cgtaaaaata aaaaaggagt gattcgaatg aattacagta aagtagcttc cgacgtaata
     2641 caagcggtcg gaaaagataa ccttattgca gctgcacact gcgctacacg tcttcgtctc
     2701 gtactaaaag atgacacgaa agtgaatcaa aaagctttag acgaaaatcc agatgtgaaa
     2761 agcacattta aaattgatgg acaatatcaa gtgattatcg gtgcaggtga tgttaatttc
     2821 gtttatgacg aacttatcaa aaaaactgga ctttcggaaa tttcaacaga tgatttaaaa
     2881 caaattgcta gtaaagataa aaaattcaac cccattatgg cattaattaa attattatca
     2941 gatatttttg ttccaattat acctgccctt gttgcaggtg gtttattaat ggcattacaa
     3001 aactttttaa cagctgctgg actttttgga ccaaaatcaa ttgaagaaat gtatcctgcg
     3061 attgaaggta tttcctcaat gatccaactg atgtccgctg caccattcat gttcttacca
     3121 attttagtag gtatttcagc tgctaaacgt tttggagcaa accaatttct tggtgctgca
     3181 attggtatga tcatgacaac accagatctt ggtggctcaa ctgaatattg gaatattttt
     3241 ggttatcatg tttcacaaac aaactatgct tatcaagtta ttccagtttt agcagctgtt
     3301 tggatattat ctatattaga aaaattcttt cataaaaaat tgccatcatc tattgacttt
     3361 acattcacgc cattattatc agtaatgatt actggatttt tgactttcac agttattggc
     3421 cctgtaatgt tattgttgtc taatggaatt acagacatta ttgtttggct atataacact
     3481 actggattta ttggaatggg tatttttgga ggaacatatt cacttatagt catgactggt
     3541 ttacatcaat catttccagc tattgaaact caattacttt cagcgtggac aaatggtatc
     3601 ggacatggag actttatctt tgtagttgca tctatggcaa acgttgctca aggtgctgca
     3661 acatttgcca tttggttctt aactaaaaat tctaagacaa aaagtcttgc ttcctctgcg
     3721 ggtgtctcag cacttcttgg aattacagag cctgccttgt tcggagttaa cttaaaatac
     3781 cgctttccat tcttttgtgc tttaattggt tcaggaattg ctacagcagt agcaggatta
     3841 cttaaagtag ttgctgtttc acttggttca gcaggtttct tgggatttct ttcaattaat
     3901 gcaacatcta ttccatttta cgtattgtgt gaattgatta gctttgtagt tgcttttgca
     3961 attacttact tctacggtaa aacaaaagct gctggtatct ttaaagcaga agaaattgct
     4021 gaacaaacac cagttaattc ttcaaaagta gcaacaacag atccaatatc tactgatgtt
     4081 gaatcaacac aaacaaaagt ttcatcagaa acaattgtta gtcccatttc tggtaaagtg
     4141 attagtcttt cttcagtaaa tgatccagtc ttttcatcag aagctatggg aaaagggatt
     4201 gccattaaac caaatggaaa cacggtccat tctccagtag atggtactat tcaactcacg
     4261 tttgaaactg gacatgctta tggtattaaa tcaaataatg gtgctgaagt tctaattcat
     4321 attggtattg atactgtatc aatgggagga aaaggattta cacaaaaagt tactactaat
     4381 caaaaagtta gaaaaggcga tattcttgga gcatttgacc gttcaatgat tgagaaagaa
     4441 ggtcttgatg atacaacaat gattattgtg accaatactt cggattattc agaagtaaat
     4501 atagtagctg aaaatacagt cactgagggt actgatttac ttgaaatcaa ataaatatat
     4561 ttccctgagt aaagatggaa ttttctttcc aaaatcaaat aatatagatt aatcaaatat
     4621 ttgagaataa cattttattt aatcttttca tatatcaatt gtatgaaaaa ttattagaat
     4681 tttaaaaaat gataaaaatt caaaaagaaa aaatagtagt tatttacttg tcatgttact
     4741 aaataaaaaa ataatttaaa tatgatatat agtcatttat ttagtgattt cattctaaaa
     4801 taaagggaga aatgtctatg ctatacggtg caattgaagc aggcggcaca aaatttgtgt
     4861 gtgccattgg cgatgaagaa atgacgatca aggaaagagt gagctttccg acaacgacac
     4921 ctgaggaaac aatgccttta gtcattgact tcttcaaaca ataccaagca gatttagcag
     4981 ggattgggat tggctcattt gggcctatcg atatccatcg cgattccgcc acttatgggt
     5041 acattacctc gaccccgaaa
//
LOCUS       DACECY010000116         4863 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.123, whole genome shotgun sequence.
ACCESSION   DACECY010000116 DACECY010000000
VERSION     DACECY010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.123"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1220)
                     /locus_tag="IWO38_002698"
     CDS             complement(<1..1220)
                     /locus_tag="IWO38_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0314716.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSFFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEM"
     gene            1281..1856
                     /locus_tag="IWO38_002699"
                     /pseudo
     CDS             1281..1856
                     /locus_tag="IWO38_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2037..3230)
                     /locus_tag="IWO38_002700"
     CDS             complement(2037..3230)
                     /locus_tag="IWO38_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0314717.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(3314..3517)
                     /locus_tag="IWO38_002701"
     CDS             complement(3314..3517)
                     /locus_tag="IWO38_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAQ0314718.1"
                     /translation="MMNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVI
                     MNGNRIQIKRKQFEKIIDTLDTI"
     gene            3774..4016
                     /locus_tag="IWO38_002702"
     CDS             3774..4016
                     /locus_tag="IWO38_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314719.1"
                     /translation="MAHQPSGSAQTECVRMPYDAPPLSCGHIYQGAIRPPGKHRNGER
                     VSRFMLPGPVRQFVTIAWMSLISRRRFMASSRHTCG"
     gene            complement(3938..4171)
                     /locus_tag="IWO38_002703"
     CDS             complement(3938..4171)
                     /locus_tag="IWO38_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0314720.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(4178..4609)
                     /locus_tag="IWO38_002704"
     CDS             complement(4178..4609)
                     /locus_tag="IWO38_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0314721.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         1325 a         1110 c         1004 g         1424 t
ORIGIN      
        1 tacatttcta caaaaaaata gtgattctgt tttgaaaatt cgatttttag ttctgcattt
       61 tcgtaaacat ccaacatttt tttcgcactg tatagaccta acccgccatt ttttttagag
      121 gtttttcctc ttttgaccat ctccgatagc gtcaaattcg ttggatttgc cttgttcgct
      181 atactaaaac gtgatccctc tttttcttcc agcagattga tcgtaatcgg actctgatcg
      241 gctacacgat gatcgaatgc gttagaaata aaaatagata aacagcgaat aaaaacaacc
      301 agatcaatcg ggattgtttt cacttcgaca ggaataacta cttggatcgg gattttctcc
      361 gattctgctt tttctagaaa ttttaaaaag acacccctga cagctgggga tacaatattt
      421 gttagctgtt gtatttgcgg tttttcaaaa atagctgcag aatgatcttc taatatttct
      481 aaaaattcca acgtttcttt tttattttcc tgaagagtaa tttttaatga aaacagtaga
      541 cttttataat catgtttgaa ttcctctaga tgatggtaat attcttcatt tatttgatac
      601 agttgaagcg attcctgatg ttgtctttct tttacttgga aactgatcaa cataatggaa
      661 aaaaaaaaag aaaaaaaact gatgactatc gacaaaagag atagaagcag ataattagat
      721 gagcggaagt gaacacttcc caataccaag taaatcacgc cccagcttaa taaaatacaa
      781 atacctccaa aatagtgttc agttggataa gtaaaaagca agttgagaaa tctcgtctgt
      841 ttgtctatac tttttactaa tagatagaaa ataaatgcga taagtagtgc aaaaaaactt
      901 gctgttccaa gatgtctctt tcctaaataa tccatcagat caaaaaccag aaaaaagctg
      961 agtccagtga tagcaaaaaa aagattactg atcaaacagc tgataaacag gtttttcgta
     1021 gtaaaataaa aaagaaacat caataataag ccaactaggt aaggaaagct cactttaggg
     1081 ataggaagta aaagtgtcac caccatcaaa gctatacaac caaagctgtt ttttactagc
     1141 agttgtttcg aaaagaataa atagatacct aaatctaaaa tcaaagtaaa ggtcaaataa
     1201 agtgtcactg ttgcgaccat cttattcctc cattgttttg tttcaaaatg tacacatatt
     1261 tttttaatag gagcctgcat atgaaaaagt attttatcat agaagataac cagtttcaca
     1321 gcgaatggat catcagagaa ttggtcaatc atcatatcga gaatatcgtg cagctcactt
     1381 ctatctctca aatcaatcag ttgatccagt tgggcactat tgataatcac gatgttttct
     1441 ttatcgatat cgacttgaag caatcactca caggtattga tgtcgccgaa caaatacgta
     1501 aaaaaaatgc acatagtaat attgtttttt tcaccgctta tacgaatttt gcagtagaga
     1561 tcatcaatcg gaaaatcctt ccgattggat atatcagaaa agacaggaat ttatctgaac
     1621 aaattcgtca agtgctagaa cagatcaatt ggaaagaaca gcagatcgta aatgtcgata
     1681 aaatctttat tgatcaaaaa ggtggaagtc tcgtcctgta tcctcgggaa attttatata
     1741 ttgaaagtat caaaggtttg aaaaaccaag ttatggtcaa aacaactaca gaagataaat
     1801 tagtgaatac ttctttaaaa caattgaaga cagagttaga aaagtatgct tactttttct
     1861 taaaaattat atgggatttt agattatctt tttagaagtt cttgaatgtc ggtatagccc
     1921 gtgtttccgg gcttttccgg catttttgat attgttagat acttacaaat gttcttatta
     1981 cctctcacca tttccccctc aaaagtagta aatggagtag taaaaccggc cttgtgtcag
     2041 gctgccagcc gttccatttc tgcccttgcg gaatcgaatg tggcgtgtgc ataatagttc
     2101 agcgtcatgg tgatattgga atgtcccata atgtactgca aggctttggg gttcattccc
     2161 gcattggcta accgggtgca gaatgtatga cgcatagcat ggggcgtcgt cgttttcggc
     2221 aacgcttctt catggctctt gttgtacttc ttcacaagcc tgccaaacat cccgccataa
     2281 tctaccgccg tcatgggata gccgttctgt ttgagaaaca ggaaattggc atacccgtca
     2341 ataatgaacg gctttgcacc tttgcggttt tttatcactc gctggaacgc ctgatacact
     2401 tcctcattca tggggatttt tctgacgccg ctttgggtct tgggttcgtc aatatagtaa
     2461 ccgacgccgg tatttctcaa aagctggtgg ctcacgatga ttacccggtt ttcaaaatcc
     2521 aaatctttgt ccgtcagtcc gcacagttcc gaaatgcgaa gccctgtccc cagcagaata
     2581 acgattgcgt cataatgctt ttggtaaacc ttatcacctt gaataaagga cagaaaactt
     2641 tcttcctgtg ctggtgtaag aggcaccttt ggttccgtat cgtcgtccag aacgtcacta
     2701 agctggaagt caaacgggtt ttttcggatg caatcgtcct gtatggcggt gtaaaaagcc
     2761 gctttcaggg aacgcttgtc gttattgatg gtcttataag aaaggccctt ttctttcatc
     2821 cgaagcgccc attcctttgc gtcggacagc ttcacactgt caatcgggca gctccccagc
     2881 cgatcttgtt ccagaatacg catcagccgt tcccggccca gtttggtgtt gtgcttcaca
     2941 ttgccccggt gcctgatctg cttggcgtag agctggcaga cggtcatttt cttgccgatg
     3001 gtgtctatcc cgtcgtcaag gtctttctgg atttctttct ccttttcccg gagtgaaata
     3061 tcctcccgtt tacctgccgg ggtcttatcc gtaggcacta acttccatga gtaaacaaac
     3121 tgcggttttc caaaggtatc aacatatttg taagcatatc gtccgtcttt tctctggctc
     3181 tctccagacc gcaaaacgcg atttttgctg tctcgtcttt tttctgacat ctcgtcatgc
     3241 tcctttccat gacggaaaga gccgcatata tgcttataaa ttattataac atatctgcgg
     3301 ccctttttca ctattagatt gtgtccaaag tgtcaattat tttctcaaac tgcttgcgtt
     3361 tgatctggat gcggttgcca ttcataatga cccagccaga ggccggattt tcctcggcca
     3421 gcttacgcaa tttgttttct ccgatacgga aatatttaga cgcttcctca atcgtcagcg
     3481 tgtatttttc ccaaataggc acatcatggt tcataatgca gccccctttc acaaattcaa
     3541 aggtttgtgt gatttagggg catagacgga cggctgctgg cacagctcca cgggactttc
     3601 acccccgcgt ggttctcacg cggccctacc cattgcctgc gacgctttta acgctcggac
     3661 tgtggctata agggagtatc atagtcctgc ctgtacgtcg tcgcccgcaa gccgccacgc
     3721 tcacattgcg gcgtggtgtt cctcgctccg cttttcccgt agggaaaagt atcatggcgc
     3781 accagccgtc cggctcggca cagacagaat gtgtccgtat gccctatgat gcgccgccgt
     3841 tatcttgcgg gcatatttat caaggtgcga tcaggccacc gggaaaacac cgaaacgggg
     3901 aaagggtaag ccgtttcatg ctgccggggc cggttcgtca gttcgtgacg atagcgtgga
     3961 tgagcttgat ttccagacgg cgcttcatgg cctcgtccag acacacatgc ggatagccgt
     4021 tttcgtcata gagcgtccgg gtacacagct tgttcatgta accgctgtaa tactgcaaaa
     4081 cgcgctctac ggctccggca tccccagcgc gggcggcctc gatcaccgaa aggggcaaaa
     4141 gctcaatgcc cttgtagttc gggttcttca tccgcgtcta acctcccttc ggcatcaacg
     4201 cctttagctg tttccgcagc tccgataagg tatttgtcct gtgccgctga acggcactcc
     4261 gggacattcc cacaaggccg ccgatctcac catctgccat atccagaaca cagtgcagga
     4321 ttaaaatact ctgctccatg ctgggcagcg cggcaaaggc ttcggcaaca agctcattgt
     4381 tgatatgtag gtcatagcca tgcgacgaaa acgtaaagct gtcgctgggg tagtgatcta
     4441 ctgtacagag cttgtccaaa tctgcctgtg acaaagagct tagtgacact tcacggtcac
     4501 ggcggcgctt catttcagcc aagtagcttt tggcctcgtt acgcagtacc gtcttgcaaa
     4561 aagcatcgaa catgcaccgc gcttcatagt cgcgggggat agcatccatc ttctcacccc
     4621 ctttctgtgg gggacttagg tggttctctc cctttccgct aacaagactt tgacaaggcc
     4681 gccagctacc cgctaaatac ctcctttttg aaaatttctc aaaaaatttt tttggggaca
     4741 gccgggccgc aaaagacgac aaaaatcgcc cggcaggtcg caaacctacc gggcgagaaa
     4801 atgctttgct atgctgctga tttacatggt atagtacata ctcataaccg gaatagccct
     4861 gtg
//
LOCUS       DACECY010000117         4808 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.124, whole genome shotgun sequence.
ACCESSION   DACECY010000117 DACECY010000000
VERSION     DACECY010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.124"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            207..305
                     /locus_tag="IWO38_002705"
                     /pseudo
     CDS             207..305
                     /locus_tag="IWO38_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(528..758)
                     /locus_tag="IWO38_002706"
     CDS             complement(528..758)
                     /locus_tag="IWO38_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAQ0314722.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(988..1806)
                     /locus_tag="IWO38_002707"
     CDS             complement(988..1806)
                     /locus_tag="IWO38_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0314723.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1967..2656
                     /locus_tag="IWO38_002708"
     CDS             1967..2656
                     /locus_tag="IWO38_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ0314724.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2670..4172
                     /locus_tag="IWO38_002709"
     CDS             2670..4172
                     /locus_tag="IWO38_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0314725.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            4185..4661
                     /locus_tag="IWO38_002710"
     CDS             4185..4661
                     /locus_tag="IWO38_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0314726.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
BASE COUNT         1608 a          701 c          857 g         1642 t
ORIGIN      
        1 gggcagcgag tgtcgacccc ttctaggggg attaaaaata cacccgtcct cacgggtgta
       61 tgggttactg atagacttga ttttaactgg tgtcagaaac ttatgtccag aaagggtgat
      121 gaaattttgg caatttacgg ctattcaaga attagcacaa gcaaccaaga ttacaaaaca
      181 caaattcaaa cattagaaaa tgctggcgca gaaataatct tcctagaaaa tcactggatt
      241 atcgaacacc tttggaagta tttttgagtt acgtaagtat tgatgatctg tctaacttaa
      301 tttgacaatt aagagtatat ttacttcaga tggtaaggca acaaaataaa atacaatagg
      361 cagatgaact aataaataaa atctgtatta aatacagata aattaatata aaaatcaaaa
      421 ggatcataaa cttttaacaa gtgatgaaat gggtggaaat tgattcttat agacagtttg
      481 tctcataaca ttgaagtatt ttgcaatttt agcattgaaa cactttttta atcttcaacg
      541 ttgttcttat aaacaagatg attgggaatc ttttgaatta acactgtata gcttagaaca
      601 ccctttatag aaaacattcg cttagcaatc ttaataattc cttgtgcccg atgtcgttta
      661 gattcagtag gttcctgctc atcgatcatg gaaaaaattt gaaggatcag atccttaata
      721 aatttattta agagaggatg atccattgct tcatccatac tcgataaact agtgataatg
      781 agctacacgt ttttctttca agttgatcaa tagcttccac gataaaaata tctttagata
      841 aaatcaagaa ccgtaggaac tacagggata tttggtcaat aagagccacc gctattagta
      901 aaaaataagc tatcaagtat gctctcatcc caagaattgc ctacttcaat cgttaaaaac
      961 cgttaggttt agctaagtgg gggtagttca ctcaatactt ttatcaataa tagagcgcaa
     1021 aactttttct cttaattcaa ctgaatcaat attattgttt tgttcataac catgaactaa
     1081 tagatcacaa atataaagtt gtgatatttt tccagctaaa gatccaccat taagaaattc
     1141 ttcaatggct gtttgtaaaa ctaagtctgc agactttcct ataggtgaat gaatatagtt
     1201 agttatagca ataatttttg cgccattttt tttagctatt ttcaatgaat cataagtatc
     1261 tttcgttttc ccagataaag aaaaaatgat tactaaatca ttaactgtta ataaagaagc
     1321 gacttgggct tgaaaatgag ggtctagtac agcttttgct tgaactccta cgcgaagaaa
     1381 catattttct aaatcaagac tagtatttcc actagatccc actccaaata tatatatgtt
     1441 ttttgattga gtgatcattt taatggcttc attcaatttt tcttcgtcta aaagcctaat
     1501 agtggaatag agagcttctg ttaaactatt agcaatttca tcatagtact ttacactagt
     1561 cggatgttct tttttttgac taaagtcttc ctttgcaatt tcaattttta aatcagaaaa
     1621 accagaaaaa ccaatttttt gacaaaagcg aataatagta gcatcgccaa cttttgtgtt
     1681 tttcttaata tcactcatcg tactataaat aatagttttc ccagaattta aaataaaatc
     1741 agcgactttt ttttcagact tcgttaaaaa tggataattt tctttaatta aatagatata
     1801 actcatacaa actcctctac aatttcaact tttctccatt atactccaaa ataataaaaa
     1861 aaactcctaa atactccatt tttatcttta cttttatgaa agcggatgct atattgtttt
     1921 tggagttatt atccaaaaat aataaaaaat tggagcgttt aggataatgg ataaaaagag
     1981 gtttttagat cttatttcaa aaggtttgat tgtttcatgt caagcattac ctggtgaacc
     2041 attttacaaa gaggaagaaa gtttaatgcc ttattttgct ttggctgctc aaagggctgg
     2101 agcagttggt atacgtgcaa attctgttcg agatattatt gaaattagaa agtgtgtatc
     2161 attaccaatg attggaatta ttaaaagaga ttatccacca gaatctccat tcattactgc
     2221 cactatgaga gaagtagatg aattagtaga aaccggtgtt gaggtaatcg ctcttgattg
     2281 cactcttaga aatcgacatg atggactatc tataaattct tttattacta aaataaaaga
     2341 aaaatatcct catcagttat ttatggcaga tatttctaca tttgaagaag gcaaaaatgc
     2401 atataaagca ggaatagatt ttgtaggaac tactttaagt ggatatactg aggaaagtaa
     2461 aaaacaagat gggccagata tagatttgat taaaaaattg gtagaagaag ggatttgtgt
     2521 aattgctgag ggaaggattc atactccaga acaagcaaaa gagataaaaa aaataggagt
     2581 tgcaggtatt gttgtgggtg gtgctattac tcgaccacag gaaattgcat caagatttgt
     2641 taaagcatta agttaggggg agaaataaaa tgtttaaaaa attttcacaa ttaggacgcg
     2701 ctttcatgtt accaattgct atattaccag tagccggatt actattggga ctgggaggag
     2761 cgttaacaaa tgagagtgct ataaatgctt atccattttt agatcaaccg tggttgcata
     2821 caattttaag tattatgagt tatgctggaa atgcagtttt tgcaaaccta gcactaattt
     2881 ttgcaattgg tattgctgta ggacttgcaa atggagataa aggaacagct ggtttagctg
     2941 gtggagtagc ttatttagta tatactgcaa ctattagtgg gtttcttgaa cttttttcag
     3001 caaaagatgc aacactagat acaggtgtag tgggatcaat tgttgttggt ggtactgttg
     3061 catttttaca caatcgttat agaaaaattg aattaccaca attcttaggt ttttttggag
     3121 gatcaagatt tattccgatt gtttctagtt tagctgcaat cattattggt tctttctttt
     3181 atttaatttg gccaccaatt caaaatggtt tagtagttgc tggtgaacaa attgcccaaa
     3241 tgggtagttt aggaactttt ttatatggtt ttttattgcg tttaacaggt gctgttggac
     3301 tacaccacac catttaccct ttgttctggt atacatcttt aggtggtacc gaaactgtag
     3361 ctggatcaac aattgctgga gcccaaaata ttttctttgc ccaactagct gatccaaacc
     3421 acactggtct gtttacttat ggtactagat tttttgcagg aagatttgca acaatgatgt
     3481 ttgggttacc agcagcatgt tatgcaatgt atcgtgcaat tcctaagaaa aatcgtaaaa
     3541 agaatggtgg gctttatttt agcggggccc ttacatcatt tttaactgga attactgaac
     3601 cagttgaata tatgttttta tttgttgctc cttggcttta tgtgattcat gcattcttag
     3661 atggattatc tttttatttt gctgatatct taaatattcg tgtagggaat tccttttcag
     3721 gtggtttgat cgacttttta ttattcggtg tacttcaagg gaatgacaaa actaattggt
     3781 taaaattaat tccttttggt attgcatggg catttatata tttctttgtt tttacattct
     3841 ttatcaaaaa atttaaggta gctattcctg gaatggaaga agaatcagaa gttcattctg
     3901 aaaaagtaat aaacaaaaat acatcattac atgaagagtc tcttatcatt attgaagctc
     3961 ttggtggaaa agaaaatatt gagtctgttg aagcttgtgc aactcgtttg cgtgttgctg
     4021 ttacgaatgg ggagattatt caaaaacaaa caataaaaga cactggtgca actgctgtct
     4081 tcgaagtgaa gggtggcatc caagctgttt ttggagggaa agctgactta ttaagtcaag
     4141 aaatcaatca aatattagga aaagataatt aggaggtttt taatatgttt gaaattttca
     4201 aaaagaaaaa acaagaaact ttttatgcac cttgttccgg aaaaatcatt tccatcacag
     4261 aagtaaatga tcctgtcttt tcacaaaaat tactaggtga tggttttgcc gtcatacctg
     4321 atggttcaaa aatttatgct ccattggctg gtcgagtaac aaatatattt cctactaaac
     4381 atgctattaa tataaaaagc aactcaggca ttgattattt gattcatatt ggaattgata
     4441 cagttgaatt acaaggagag cctttttcga tttgtgttca agaaaatgat gaggttactc
     4501 ctgaaacctc gttggttgat gtggattttg acaaaataat aaaatctggt aaagatactt
     4561 ctgttatagt tgtttttccg gagaaaaatc agttcaatga tttaataata gataatggtc
     4621 gaattgctca tgggaattca tgtggaaaaa tcacttttta aaaaagtgta tataaaaagc
     4681 tataaaataa gctaaatagg atatttgtca aatgaagttg gtgaacagaa agaactgttt
     4741 tctttctgtt cattttttta ttttattagt aatgattccc catgccaaat catgatccgt
     4801 aatttaaa
//
LOCUS       DACECY010000118         4807 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.125, whole genome shotgun sequence.
ACCESSION   DACECY010000118 DACECY010000000
VERSION     DACECY010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.125"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..411
                     /locus_tag="IWO38_002711"
     CDS             <1..411
                     /locus_tag="IWO38_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314727.1"
                     /translation="GLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAP
                     QEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLT
                     ELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            509..1882
                     /gene="tet(L)"
                     /locus_tag="IWO38_002712"
     CDS             509..1882
                     /gene="tet(L)"
                     /locus_tag="IWO38_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ0314728.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            2446..3708
                     /locus_tag="IWO38_002713"
     CDS             2446..3708
                     /locus_tag="IWO38_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAQ0314729.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            3953..>4807
                     /locus_tag="IWO38_002714"
     CDS             3953..>4807
                     /locus_tag="IWO38_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ0314730.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHKK"
BASE COUNT         1660 a          665 c          981 g         1501 t
ORIGIN      
        1 ggcttatact atagccctgt tagtacccca gcagattttc ggatgcttgc tcctattgta
       61 ttggaacaag tcttaaaaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 atttatgcgc cacaggaata tctttcacga gcatacaacg atgctcctaa atattgtgcg
      181 aacatcgtag acactcaatt gaaaaataat gaggtcattc ttagtggaga aatccctgct
      241 cggtgtattc aagaatatcg tagtgattta actttcttta caaatggacg tagtgtttgt
      301 ttaacagagt taaaagggta ccatgttact accggtgaac ctgtttgcca gccccgtcgt
      361 ccaaatagtc ggatagataa agtacgatat atgttcaata aaataactta gtgtatttta
      421 tgttgttata taaatatggt ttcttgttaa ataagatgaa atatttttta ataaagattt
      481 gaattaaagt gtaaaggagg aattaattat gaagtgtaat gaatgtaaca gggttaaata
      541 caaccaaatt ttaatttggc tttgcgtttt atcttttttt agcgtattaa atgaaatggt
      601 tttgaacgtc tcattacctg atattgcaaa tgattttaat aaaccacctg cgagtacaaa
      661 ctgggtgaac acagccttta tgttaacctt ttccattgga acagctgtat atggaaagct
      721 atctgatcaa ttaggcatca aaaggttact cctatttgga attataataa attgtttcgg
      781 gtcggtaatt gggtttgttg gccattcttt cttttcctta cttattatgg ctcgttttat
      841 tcaaggggct ggtgcagctg catttccagc actcgtaatg gttgtagttg cgcgctatat
      901 tccaaaggaa aataggggta aagcatttgg tcttattgga tcgatagtag ccatgggaga
      961 aggagtcggt ccagcgattg gtggaatgat agcccattat attcattggt cctatcttct
     1021 actcattcct atgataacaa ttatcactgt tccgtttctt atgaaattat taaagaaaga
     1081 agtaaggata aaaggtcatt ttgatatcaa aggaattata ctaatgtctg taggcattgt
     1141 attttttatg ttgtttacaa catcatatag catttctttt cttatcgtta gcgtgctgtc
     1201 attcctgata tttgtaaaac atatcaggaa agtaacagat ccttttgttg atcccggatt
     1261 agggaaaaat atacctttta tgattggagt tctttgtggg ggaattatat ttggaacagt
     1321 agcagggttt gtctctatgg ttccttatat gatgaaagat gttcaccagc taagtactgc
     1381 cgaaatcgga agtgtaatta ttttccctgg aacaatgagt gtcattattt tcggctacat
     1441 tggtgggata cttgttgata gaagaggtcc tttatacgtg ttaaacatcg gagttacatt
     1501 tctttctgtt agctttttaa ctgcttcctt tcttttagaa acaacatcat ggttcatgac
     1561 aattataatc gtatttgttt taggtgggct ttcgttcacc aaaacagtta tatcaacaat
     1621 tgtttcaagt agcttgaaac agcaggaagc tggtgctgga atgagtttgc ttaactttac
     1681 cagcttttta tcagagggaa caggtattgc aattgtaggt ggtttattat ccataccctt
     1741 acttgatcaa aggttgttac ctatggaagt tgatcagtca acttatctgt atagtaattt
     1801 gttattactt ttttcaggaa tcattgtcat tagttggctg gttaccttga atgtatataa
     1861 acattctcaa agggatttct aaatcgttaa gggatcaact ttgggagaga gttcaaaatt
     1921 gatccttttt ttataacagg aattcaaatc tttttgttcc attaaagggc gcgattgctg
     1981 aataaaagat acgagagacc tctcttgtat cttttttatt ttgagtggtt ttgtccgtta
     2041 cactagaaaa ccgaaagaca ataaaaattt tattcttgct gagtctggct ttcggtaagc
     2101 tagacaaaac ggacaaaata aaaattggca agggtttaaa ggtggagatt ttttgagtga
     2161 tcttctcaaa aaatactacc tgtcccttgc tgatttttaa acgagcacga gagcaaaacc
     2221 cccctttgct gaggtggcag agggcaggtt tttttgtttc ttttttctcg taaaaaaaag
     2281 aaaggtctta aaggttttat ggttttggtc ggcactgccg acagcctcgc agagcacaca
     2341 ctttatgaat ataaagtata gtgtgttata ctttacttgg aagtggttgc cggaaagagc
     2401 gaaaatgcct cacatttgtg ccacctaaaa aggagcgatt tacatatgag ttatgcagtt
     2461 tgtagaatgc aaaaagtgaa atcagctgga ctaaaaggca tgcaatttca taatcaaaga
     2521 gagcgaaaaa gtagaacgaa tgatgatatt gaccatgagc gaacacgtga aaattatgat
     2581 ttgaaaaatg ataaaaatat tgattacaac gaacgtgtca aagaaattat tgaatcacaa
     2641 aaaacaggta caagaaaaac gaggaaagat gctgttcttg taaatgagtt gctagtaaca
     2701 tctgaccgag atttttttga gcaactggat ccaggagaac aaaaacgatt ttttgaggaa
     2761 agttataaat tattttccga acgatatggc aagcaaaata ttgcttatgc aacagttcat
     2821 aatgatgagc aaacccctca catgcattta ggtgttgtgc ctatgcgtga tggaaaactg
     2881 caaggaaaaa atgtgtttaa tcgtcaagaa ctgttatggc tacaagataa attccccgag
     2941 catatgaaaa aacagggttt tgagttgaag cgtggtgaac gtggctctga ccgtaaacat
     3001 attgagacag ctaaatttaa aaaacaaact ttggaaaaag agattgattt tctagaaaaa
     3061 aatttagcag ttaaaaaaga tgaatggact gcttatagcg ataaagttaa atcagattta
     3121 gaagtaccag cgaaacgaca catgaaaagt gttgaagtgc caacgggtga aaagtccatg
     3181 tttggtttgg gaaaagaaat aatgaaaaca gaaaagaaac caaccaaaaa tgttgttata
     3241 tcggagcgtg attataaaaa cttagtgact gctgcgagag ataacgatag gttaaaacag
     3301 catgttagaa atctcatgag tactgatatg gcgagagaat ataaaaaatt aagtaaagaa
     3361 catgggcaag ttaaagaaaa atatagtggt cttgtagagc gatttaatga aaatgtaaat
     3421 gattataatg agttgcttga agaaaacaag tctttaaagt ctaaaataag cgatttaaag
     3481 cgtgatgtga gtttaatcta tgaaagcact aaggaattcc ttaaggaacg tacagacggc
     3541 ttaaaagcct ttaaaaacgt ttttaagggg tttgtagaca aggtaaagga taaaacagca
     3601 caattccaag aaaaacacga tttagaacct aaaaagaacg aatttgaact aactcataac
     3661 cgagaggtaa aaaaagaacg aagtcgagat cagggaatga gtttataaaa taaaaaaagc
     3721 acctgaaaag gtgtcttttt ttgatggttt tgaacttgtt ctttcttatc ttgatatata
     3781 tagaaataac gtcattttta ttttagttgc tgaaaggtgc gttgaagtgt tggtatgtat
     3841 gtgttttaaa gtattgaaaa cccttaaaat tggttgcaca gaaaaacccc atctgttaaa
     3901 gttataagtg accaaacaaa taactaaata gatgggggtt tcttttaata ttatgtgtcc
     3961 taatagtagc atttattcag atgaaaaatc aagggtttta gtggacaaga caaaaagtgg
     4021 aaaagtgaga ccatggagag aaaagaaaat cgctaatgtt gattactttg aacttctgca
     4081 tattcttgaa tttaaaaagg ctgaaagagt aaaagattgt gctgaaatat tagagtataa
     4141 acaaaatcgt gaaacaggcg aaagaaagtt gtatcgagtg tggttttgta aatccaggct
     4201 ttgtccaatg tgcaactgga ggagagcaat gaaacatggc attcagtcac aaaaggttgt
     4261 tgctgaagtt attaaacaaa agccaacagt tcgttggttg tttctcacat taacagttaa
     4321 aaatgtttat gatggcgaag aattaaataa gagtttgtca gatatggctc aaagatttcg
     4381 ccgaatgatg caatataaaa aaattaataa aaatcttgtt ggttttatgc gtgcaacgga
     4441 agtgacaata aataataaag ataattctta taatcagcac atgcatgtat tggtatgtgt
     4501 ggaaccaact tattttaaga atacagaaaa ctacgtgaat caaaaacaat ggattcaatt
     4561 ttggaaaaag gcaatgaaat tagactatga tccaaatgta aaagttcaaa tgattcgacc
     4621 gaaaaataaa tataaatcgg atatacaatc ggcaattgac gaaactgcaa aatatcctgt
     4681 aaaggatacg gattttatga ccgatgatga agaaaagaat ttgaaacgtt tgtctgattt
     4741 ggaggaaggt ttacaccgta aaaggttaat ctcctatggt ggtttgttaa aagaaataca
     4801 taaaaaa
//
LOCUS       DACECY010000119         4772 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.126, whole genome shotgun sequence.
ACCESSION   DACECY010000119 DACECY010000000
VERSION     DACECY010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.126"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..369
                     /locus_tag="IWO38_002715"
     CDS             1..369
                     /locus_tag="IWO38_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0314731.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            549..1490
                     /locus_tag="IWO38_002716"
     CDS             549..1490
                     /locus_tag="IWO38_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ0314732.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1559..2290
                     /locus_tag="IWO38_002717"
     CDS             1559..2290
                     /locus_tag="IWO38_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ0314733.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2434..4614
                     /locus_tag="IWO38_002718"
     CDS             2434..4614
                     /locus_tag="IWO38_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ0314734.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4634..>4772
                     /locus_tag="IWO38_002719"
     CDS             4634..>4772
                     /locus_tag="IWO38_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ0314735.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HL"
BASE COUNT         1595 a          847 c         1067 g         1263 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata
      121 attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt
      181 gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat
      241 atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag
      301 cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca
      361 ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt
      421 taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca
      481 aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga
      541 agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag
      601 aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc
      661 tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct
      721 atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa
      781 gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa
      841 agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat
      901 ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat
      961 atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc
     1021 cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta
     1081 ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg
     1141 tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat
     1201 tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag
     1261 ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt
     1321 gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt
     1381 taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg
     1441 gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag
     1501 ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat
     1561 gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact
     1621 tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg
     1681 aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc
     1741 catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga
     1801 ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc
     1861 aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc
     1921 agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt
     1981 tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt
     2041 tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt
     2101 tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca
     2161 ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt
     2221 ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca
     2281 tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt
     2341 ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa
     2401 agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc
     2461 attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt
     2521 aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact
     2581 ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac
     2641 ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc
     2701 gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt
     2761 ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca
     2821 ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat
     2881 attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt
     2941 attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat
     3001 aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat
     3061 gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct
     3121 actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca
     3181 attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc
     3241 gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa
     3301 ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac
     3361 ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg
     3421 gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc
     3481 gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct
     3541 tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc
     3601 agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat
     3661 tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa
     3721 gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa
     3781 gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa
     3841 catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt
     3901 aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga
     3961 ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc
     4021 gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc
     4081 ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa
     4141 agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt
     4201 acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta
     4261 tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat
     4321 atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg
     4381 aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt
     4441 gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc
     4501 aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc
     4561 gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga
     4621 ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa
     4681 aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg
     4741 cgggcgctat catttaaacg atcatcattt ag
//
LOCUS       DACECY010000120         4759 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.127, whole genome shotgun sequence.
ACCESSION   DACECY010000120 DACECY010000000
VERSION     DACECY010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.127"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            315..2159
                     /locus_tag="IWO38_002720"
     CDS             315..2159
                     /locus_tag="IWO38_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0314736.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSSTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            2214..3680
                     /locus_tag="IWO38_002721"
     CDS             2214..3680
                     /locus_tag="IWO38_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0314737.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            3717..4559
                     /locus_tag="IWO38_002722"
     CDS             3717..4559
                     /locus_tag="IWO38_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0314738.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
BASE COUNT         1555 a          764 c         1022 g         1418 t
ORIGIN      
        1 gttggaagat attcttagaa aaaagacaaa acttttagaa cacgaaaact atattgaacg
       61 aataaaatga aaaaatgatt aaaaaatcat tttattattt acgtttacat tctaatatgt
      121 taaattgaat ataacttaaa caggcatgtg actggtaatg caggcaggac ctaaataagt
      181 tgaataacaa tgtatgcgtt tcaatacacg tttgccttgt tgtcaattta tttagtttct
      241 gcctgttttt tgtgttgagg atggccactt cgactcaaaa gttcatcact caaaaaagaa
      301 aagagggaaa caccatgaat tacaaacaaa cagcagaaga agtattgcag tacatcggtg
      361 gggaagaaaa catatcccat ttggagcatt gctcaactcg tttacgtttt acgttaaaag
      421 acaacagcaa ggtaaatatg agccagttag aaaaagtgga tggcgttatg ggcgttcgcc
      481 aaaatgtgca atgccaagta atcattggaa acgatgttgt tgaagtgtat gatgaagtga
      541 aagcgttatt aggtgacaat gtttcaagta ctgaagaagc accaaaacaa aaacaaaaat
      601 taggttcagt tctattagat tttattattt ctattttcca acctttaatt cctgcgattg
      661 caggtggagg agtattgaag tccttgttgc tactagcttc tatcttaggg ctgattagtg
      721 accaatctca aacctatcag attttaaatt tggtcggcgg ggcaccgctt tatttcttgc
      781 ctattctggt ggcaatgact acggctaata aattgaaagt caatcaattg gttgcagtat
      841 ctgctgtcgg tgcattgatc cttcctgaaa tgacagctat gttggcggaa ggtgctacat
      901 tcttatcttt tggattggat aatatagcgt atgcatctca agtattccca gccatcttaa
      961 ctgttttatt ttatgctcaa cttgaaaaat ggtttacaaa agtttcacca aaaccaattc
     1021 gtattttctt tgtacctatg atgagtttat tgattacagt tcctattgct ttgttgcttc
     1081 taggaccatt aggttataat gtaggaacag ctttctcttc tatcattgtg tctttgtaca
     1141 atcaatttgg atgggttgca accggagtct tagcagctgt actgccatta atggttgtaa
     1201 cgggtatgca taaagcaatg atcccttacg ctgtttcatc catgagtgaa ttgggtgcag
     1261 aacttcttta tctcccggct tcattggcac acaatatttc agaagctggc gcatgctttg
     1321 ctgtcagtat caaaacaaaa gacaaaaaat tgaaatctac ggctgtttct gcgggtatat
     1381 ctgcattatt cggaattact gaaccagctc tttacggggt aacgattctg cacaaacgtg
     1441 ttttatatgc cgttatgttt tctagcctga tcgggggagc atttgcagga atcgttgcac
     1501 ttcaagcctt tgctttagtg ggtcctggga ttgcaagtat cacgatgttc gctgacaaag
     1561 ctgacccaat gaatctggtt tgggcatttg cagtatcggg gatttcattt gtggttgctt
     1621 ttgctgcagc gttggttctt tataaagata aagaagttgt caatgaagtt gaaactaaga
     1681 tggaaacggc cgttgtttca gatgctgttc tttcaagtcc cgtgaatgga aaagtaatct
     1741 cactatcaga agtaaaagac actgtttttt ctagtggtat ggtaggtgat ggagtagcaa
     1801 tcattcctga agaagggata ttagtagctc ctgaagatgg agaaatcaca atggtatttg
     1861 aaacgaagca tgctattggt ttaaaaacgg ctaatggtgc agaacttctt tttcatattg
     1921 gtattgatac tgttcagtta gaaggaaaag ggtttgaggc atttgttcgt tctggtgaaa
     1981 aagtagccaa aggacaaaag ctgattgcat ttgatattga tttaatcaaa gaagcaggat
     2041 tcgaccccac agttatctgt gtggtaacca ataaggataa ttacactatc cgttcattta
     2101 acctagaaaa attggtaacc aatgatagtg atctcttagc tatcagtccg atagcttaaa
     2161 cacaagatag tgaaaaaaat atattgaatg aaggaataga ggagaataga ataatgacaa
     2221 acacaacaag ttttccaaaa ggatttttat ggggcggcgc gatagcagca aatcaagtag
     2281 aaggtgcttg gaatgtagac ggtaaaggtc tttcagtagc ggatatggct acttataaac
     2341 caaatttaga caacaaagat tttgctgctc atatgcatgt caccctgcag tctataaaag
     2401 aagctagtga agacaaatct gataaatggt acccaaaaag aaggggcgtt gatttttatc
     2461 atcactataa agaagattta gctttatttg ccgaaatggg ctttaaaacc ttgcgcctct
     2521 ctattgcttg gactcgcttg ttccctacag gagaagagct ggaaccgaat gaaaaagggg
     2581 ttgcatttta tgaaaacgtc tttaaagaaa tgcaacgttt aggaattgag ccgattgtga
     2641 cactatctca ttatgaaatg ccattagctt tagcagttaa atataatggc tgggtggagc
     2701 gtcgagtaat tgatgatttt gttcgcttcg ctaacgtttg ttttgatcgc ttcggcaaat
     2761 acgtaaaata ttggctgaca tttaatgaga tagacagcat ccaccgtcat ccatttacaa
     2821 cagctggaat tatccaagag aaaagcgcag aaggtcaaga agctcaagat atctatcaag
     2881 cattgcatca tcaatttgtt gcgagtgcgt tagtgacaaa agatgctcat gaaaaaattc
     2941 caggaagcca agttggttgt atgttaacga aattgatgac ttatccatta acttgcgcac
     3001 cagaagacgt tgagctgacc ttgaaaaaga acttagaaaa taatttttat gcagatgttc
     3061 aagttaaggg agaatatcct gctctaatcg aaaaggattt ggaaaatcgg ggaattcaga
     3121 ttaaaatgga agatgaagat cgagaaattt taaagaaaca taccgtagat tttgtttctt
     3181 tcagctatta catgtccatg gcagaatcag ctgatccaaa cgcagaacgc acaccgggaa
     3241 atactgtttt aggggttaag aatcctcatt tgccttcaac agattggggc tggcaaatcg
     3301 atccaaaagg tttgaaaatt tcgttgatcg aactttatga tcgttaccaa gtaccgttga
     3361 tgattgtgga aaatggtatg ggagcagtcg atcaggtagt agatggtaaa atccatgatc
     3421 cgtaccgcat cgactacttt aaagcacact tccaacaaat gaaagaagca atcgatgaag
     3481 gtgtagattt gatagcttat actagctggg gaccaattga tttagttagt gcaggaactt
     3541 ctcaaatgtc taaacgatat ggatttattt atgtggatgc agacgatgaa ggaaatggga
     3601 catataaccg cttgaaaaag gattcgttca actggtacaa agaagtgatc gaaacaaacg
     3661 gtgcatcctt attcgagtag tctcgatctt tttaagcagc gataggggga taatgagtga
     3721 tttcaataaa aaaagtattg aattcaagtg tggtactggt tgaagaaaat ggcaaagaaa
     3781 tgattgcatt aggtaaaggg ataggctttg gacagaaagc tggggatgaa atatcaggag
     3841 ataaagtaga taaaatattt ctgccttttg aagaaaagaa atcttcacaa ctattagagt
     3901 tagttgaaga aatacctatt gaattttttg aaatcactcg tactatcgtt tttgaagcag
     3961 aaaaaggatt agataaaaaa ctgaatcctg gagtctattt aaccttaaca gaccacctgc
     4021 attttgcagt tgaacgcgca caaaaagggg taaatattgc aaaccgtttg tactgggaaa
     4081 taaaaatcta ctatcccaaa gaatttcaaa ttgggaagta cgcactcacc cttttgaacg
     4141 agcaatatgg aattgatctt cctaaagagg aagcctcaaa cattgctttc catttgatca
     4201 atgcacaatc ggaagatcct gatagcggcg ctggattcaa gtatgcaaaa atgatcggcg
     4261 ggatcgtcaa cttggtccgg tattcgttgc agaaagatat cgatactaat tcgatccatt
     4321 atgcccgctt cattacacat gtacgtttct ttgtagaaag atattattct gatgctttgt
     4381 tggatgatgg tgaggaagaa ttctacaatc aaatgtggat cttatacccg tctgccatgg
     4441 aaagcgcagc taagatcaaa gactacattg aaaaagttta tcataaaaca attccgaacg
     4501 atgaagttgt atacctaggt gtccacatca atcggttgat gaaacatgct aatgggtgag
     4561 cgctcagtaa tactattgat gcttttaaaa tctttagaga tggattatag taaaaacaag
     4621 gtgatttcca gctgtttgtt atctataaga gacatttaaa aaggattaac gtaagataat
     4681 aaaaaatctc ggctctttgt caaatgatgt tggtaaagag aaatcttaga ataatttgga
     4741 cagaacagaa agaagaaaa
//
LOCUS       DACECY010000121         4582 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.128, whole genome shotgun sequence.
ACCESSION   DACECY010000121 DACECY010000000
VERSION     DACECY010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.128"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(37..1257)
                     /locus_tag="IWO38_002723"
     CDS             complement(37..1257)
                     /locus_tag="IWO38_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0314739.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1882)
                     /locus_tag="IWO38_002724"
     CDS             complement(1277..1882)
                     /locus_tag="IWO38_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0314740.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="IWO38_002725"
     CDS             complement(1931..2863)
                     /locus_tag="IWO38_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ0314741.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="IWO38_002726"
     CDS             complement(3284..4144)
                     /locus_tag="IWO38_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0314742.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
     gene            complement(4291..>4582)
                     /locus_tag="IWO38_002727"
     CDS             complement(4291..>4582)
                     /locus_tag="IWO38_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ0314743.1"
                     /translation="VTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNNLIEQDHRPVK
                     RRNKFYRSLRTGSTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT         1295 a          918 c          763 g         1606 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acccgtaccg catctgttgc cccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgagaatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aattggttaa gatatcggtt ctgttgcaaa gttttaaata
     4261 aagaataaaa tcccttacgg tatctatgat ttaagctggg attcccaata ataccttgat
     4321 ttcagtacag accgaaaacc cgaagagagt gccttctttt cgggttttct tatataatcc
     4381 tcgaatggct tccatgcctt taatcgtggt agagccagtg cgtaaacttc gatagaattt
     4441 attgcgtctc tttactggac gatggtcttg ttcaatcaaa ttattcaggt atttaatggt
     4501 acgatgttct gtcccttgat aaaagccgta ttcttttagt ttcttaaagg cacttgtaat
     4561 agagggggct ttatctgtga ct
//
LOCUS       DACECY010000122         4509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.129, whole genome shotgun sequence.
ACCESSION   DACECY010000122 DACECY010000000
VERSION     DACECY010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.129"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            161..391
                     /locus_tag="IWO38_002728"
     CDS             161..391
                     /locus_tag="IWO38_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ0314744.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            488..1789
                     /gene="murA"
                     /locus_tag="IWO38_002729"
     CDS             488..1789
                     /gene="murA"
                     /locus_tag="IWO38_002729"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ0314745.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1802..1978
                     /locus_tag="IWO38_002730"
     CDS             1802..1978
                     /locus_tag="IWO38_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ0314746.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2113..2409
                     /gene="yidD"
                     /locus_tag="IWO38_002731"
     CDS             2113..2409
                     /gene="yidD"
                     /locus_tag="IWO38_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ0314747.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2473..2856
                     /locus_tag="IWO38_002732"
     CDS             2473..2856
                     /locus_tag="IWO38_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0314748.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            3025..3909
                     /locus_tag="IWO38_002733"
     CDS             3025..3909
                     /locus_tag="IWO38_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0314749.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4026..4259
                     /locus_tag="IWO38_002734"
     CDS             4026..4259
                     /locus_tag="IWO38_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ0314750.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4347..4427
                     /locus_tag="IWO38_002735"
     tRNA            4347..4427
                     /locus_tag="IWO38_002735"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4381..4383,aa:Tyr,seq:gta)
     gene            4434..4505
                     /locus_tag="IWO38_002736"
     tRNA            4434..4505
                     /locus_tag="IWO38_002736"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4466..4468,aa:Gln,seq:ttg)
BASE COUNT         1516 a          703 c          992 g         1298 t
ORIGIN      
        1 tttgtctcag tccctttttt tgcatatttt tagcatttat ttcctaaaaa atgtaagggg
       61 attcctatat ttttaagtat acttgttttt ccttttatac attttatggt attatcattt
      121 tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga
      181 tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg
      241 ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat
      301 cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc
      361 tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc
      421 aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg
      481 gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat
      541 tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg
      601 tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat
      661 tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgacgc
      721 ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat
      781 cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg
      841 atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc
      901 aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac
      961 gatttattta gatttcccta gtgtgggtgc tactcaaaat atcatgatgg ctgccgtaaa
     1021 agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc
     1081 aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat
     1141 tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc
     1201 aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat
     1261 ctctgaacat aactgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga
     1321 agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac
     1381 gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt
     1441 tgcagaaggt acaagtgtgg tgactgaaac agtgtttgaa aatcgtttcc aacatttaga
     1501 agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg
     1561 aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt
     1621 agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga
     1681 ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg tcgaacgtgt
     1741 agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg
     1801 aatgagtaac caacgatata tcttattaac tttgataaag attttagtgg tgatcttatt
     1861 gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa
     1921 tccatttaaa gtattccagc caagtttgtg gattcatatt cgagactttt ttcattgata
     1981 aataagaatc acagtggttc tagtcgatat gaagtgagcc atgaagcaga acatgataaa
     2041 agaaaacaga agggaacgtt gacgttagtc gcgatccctt tttttagtaa aagaaaagaa
     2101 ggaatctaat tgatgaagaa gatattgatt tatttagtta gaggatatca gcgctttatt
     2161 tcgcctgcat ttccacctag ctgccgttat tacccaacct gttccaatta tatgataaaa
     2221 gcaattcaaa cccatggtgc gctaaaaggg acggtaatgg ggactgcacg gatcttacgc
     2281 tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat tgacattccg attaacgcga
     2341 aataagaaag ataaaaaaga accagattat aaaaaggttg tgaaaaatac gggtagcact
     2401 gagcgataaa gaaaaagagg aaatttattg tcaaagtaac aactccgcaa ctacttatat
     2461 gggggacaaa aaatgatcga agaactaaac aagaaagatt taccatggtc cttgttatta
     2521 gatgcagatc ctgataaaga aaaagttcaa gtatatgttt ccagagggag tggctggata
     2581 tggaaagaaa aagaaaaaac aataggtgta ctgatctatg tggcaaggga aaaagaatac
     2641 gaaatcgtca atgtagcagt aaccccaacg tatcaaggaa aggggattgg cggaaaatta
     2701 cttgagacag cttttcagaa gctttcacag ctagcttcaa accaaactcg gatcattata
     2761 cgtactggca ctactagtag cgctgcgtta cacctctatc aaaaaatggg ctatgtggag
     2821 attggtcgag tcaaagacta ttttatccat cattaatctg agcccatttt tgaaaatgga
     2881 gatcaattaa gggatcaagt gattttagca attcacttac ctaaaaatag ctttaactaa
     2941 taaactttca attcgagaaa atgctttcaa atgctaaata gatacattac aatagcttta
     3001 gcaaacaaaa aggagaatat ttttatggga atattagttt ttgattttgg tggatcggct
     3061 gtcaaatatg gttgttggga tggcaaagag attaaaggta agggacaatt tgcaacgcct
     3121 gaatcttggg aagaaatgaa aacgcaatta tttcaagttt ataaaaaaat actcgtttct
     3181 tttgaagtcg aaggaattgg aatcagctct cctggagtag tgaataatga gaaacaggtg
     3241 attgagggaa tcagtgcgat tccttatatt catggattta atattttccg agatttggaa
     3301 actctgtttc aactcccagt aacgattgaa aatgacgcca actgtgccgg aatggctgaa
     3361 ttttatgaag gtgctgcccg tgattatcaa gatgttgctt ttgttgtggc aggaactggg
     3421 attggcgggg cgttgttcac gaaaggacaa ataaataaag gggcacatct ttacggtgga
     3481 gaatttgggt tgatgttctt agatggcgat aagacattca gtaaacttgg tacagcggta
     3541 cagatggctt ggcgttattg tgaacgcaaa ggtctcgata aaaatgctta taccggaaaa
     3601 gatgtctttg aattagcaga aactggagat gcaatagcaa aagaagaagt tgagagtttt
     3661 tatacgtatc ttacaaaggg attattcagt attcaatttt cgtttgatcc agaagtgatc
     3721 gtattaggcg gcggggtttc tgcaaaagaa gggctcattg atgagatcaa aagccgaatg
     3781 aagaagctga cagaacaatt cgatctgcat gatttcgaac cgcagatttt attatgtgag
     3841 tacagaaacg atgctaatct tgtaggtgca gcagcaaact atctcgctgt acgtcaagga
     3901 gagaattaag agctttagag aaggtgcagc ttgcataaaa gaagagaatg aaatgaatag
     3961 gagattttta cctaaggaat gttttatttg ctgcagtatg ataaaatatc tgagaggtga
     4021 aaatgatgtc aaaaaaaaat aaagatattg aagtgcgtat agaagaagtg aaaaaagata
     4081 tcaaaggtaa gaattatgaa gtcactcagt tatttatagg gaaaaaaatg attggtgaga
     4141 tcttagccta tgggccaaaa gaatatgaaa ttttctttgg tgaagaagat tttggaaaag
     4201 aaaaaagtct tgaaaatgcg atagaaacag tgattcgaca ctggaatctg cacgaataaa
     4261 aaaatatcaa aaaaactttc aataaagggt tgcaaaaatc tggaaaatta ggtattataa
     4321 ctaagtcagg ttgatacaaa cctttcggag gagtagcgaa gtggctaaac gcgacggact
     4381 gtaaatccgt tccttagggt tcagtggttc gaatccactc tcctccattt cattggggta
     4441 tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga atccagctac
     4501 cccagtata
//
LOCUS       DACECY010000123         4412 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.130, whole genome shotgun sequence.
ACCESSION   DACECY010000123 DACECY010000000
VERSION     DACECY010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.130"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..115)
                     /locus_tag="IWO38_002737"
     CDS             complement(<1..115)
                     /locus_tag="IWO38_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ0314751.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDR"
     gene            194..607
                     /locus_tag="IWO38_002738"
     CDS             194..607
                     /locus_tag="IWO38_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314752.1"
                     /translation="MLIGGYFLLNLDSVNSNAQIKDQRVAEHNNKKMSLTKNIYSMSD
                     EELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSELVSYKQNHRQFIDV
                     NTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            621..1310
                     /locus_tag="IWO38_002739"
     CDS             621..1310
                     /locus_tag="IWO38_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314753.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1435..3786
                     /locus_tag="IWO38_002740"
     CDS             1435..3786
                     /locus_tag="IWO38_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ0314754.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3866..4261
                     /locus_tag="IWO38_002741"
     CDS             3866..4261
                     /locus_tag="IWO38_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314755.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1540 a          574 c          882 g         1416 t
ORIGIN      
        1 cacgatcata taagatttct tgaacttcac gatagctaag gttataacga agatagtagc
       61 ccacggctac aataatcaca tcctgctgaa attgctttcc tttaaaatga ttcatcgtta
      121 ttcctcctgc tatctttttc tattattcta tcttatttga tagtagattt aaaactttgc
      181 aacagaacct acaatattaa ttgggggcta ttttcttttg aatttagatt ctgttaattc
      241 aaacgctcaa ataaaagatc aaagagtagc tgaacataat aataaaaaaa tgagtttgac
      301 caaaaatatc tattctatgt cagatgaaga attgtataaa gaatttggtg atttatccgg
      361 ggaacagtta gaagaaagta ttccaggaat tagtgactca gataaaattg ttgtcttgtc
      421 tgctggtggg caattaatgg gagatggcaa atccgaatta gttagttata aacaaaatca
      481 cagacagttt attgatgtta atactgattc caacgctatt tcgattaagg atctaaaaga
      541 gcttttaaat agtaaaagaa atgaattaaa gcagataaaa gaaactaatt cagtggaatt
      601 agagtaaagg aagattcatt atgaaaaaga tttttggtgt ttctgttggg ctacttatga
      661 tgttaacatt tggaaatgca gtatatgctg ctgagaaaga agttatttca gatccagagc
      721 agtacgcctc acgatacttt gctgattttg aagaagaaga taaagtactt gcattgccag
      781 aaggtggttt tttaaaaggg gaagctaaaa ttgtatctat ggataatcca gatataattc
      841 aaggttcata taattcagaa actgatcctg cttcagtttc agtgacagaa gcaaaaaaaa
      901 ttgttgctga aaaattcaaa aatgatattg aaactactcc aattatgcct cgaggagcat
      961 ttccacccga tgttggtact agcggacacc ttcttgtact agcaggagga ggaagctata
     1021 aatcacagcc atttagtggt gcgggatggc gttttggagg tatgggtgta aaaccagctg
     1081 atggtacagg caacttttta cgttggaaat cctacattga cgatggaaga gtaggaaatg
     1141 taaatcaagc caacgcaact aaatctggtt ctattcaagg aatggcattg tatgctcgtg
     1201 aaggattcaa atactttgca ccaaacgggc ctggttggac aatgaatata ggtatttatt
     1261 acacttataa tcctgttgca ggtacgtatt atgttgttca aaatatttaa taaaacagca
     1321 caaagagatt tctttgtgct gttttattat ttttagttgt tgacaaacac taaaatataa
     1381 aatataatca acacataaac gaacgtatgt tcggcaagaa agcaggggat ttttatggat
     1441 gagatggttt taagtactca gaaatggcta aacaagaaat acagtaatgt tacagggttt
     1501 gataaggttc cagaaaatgg gcgaacaggg tggcctacaa tctatggact tattgaagga
     1561 ctacaagtag aacttggtat tactaatcta gtagctaatt ttggacctac cactgaaaaa
     1621 atgtacgata atcaggttac tcctaaatgg ggcaaaaatc tacctaaaaa tattgtattt
     1681 cttattcaag gtgccttttg gtgtaagggg attaatccag gtggcttcga tggggtatat
     1741 actccatatt tagatactgc tgtaaaagag ttgcagactg atgcagggtt taatggaagc
     1801 gctgtaactg gagttttgga tgcgaaatgg gccaaagcgt tatttgatat gagtgctttt
     1861 gttcttgttc ccggcggaga tagtaaaatt agaaaaatgc agcaatggtt aaatgtaaat
     1921 tatttagaat acacgggaat aatgccttgt gatggtattt atcaaagaaa ttcaaatcaa
     1981 gcattaattt ttgcattaca agctgaacta ggatattctc ccagtgaagc aacagggtct
     2041 tatggtaatg ggacaactag taaaacttat cctgtgtctg aaggtaactc gggaaactat
     2101 gtaagaataa ttcaatatgg attatatgtt aatggttatt ttgaagacgg tacattcgat
     2161 ggtattttca ctaaatatat gggtttagaa gtactggcat tccgaaaatt tatggtattg
     2221 cctgaatata ctgaaatagc agatgttgtt gtaataaaag gactattgtc tagcgctgga
     2281 gatattagaa gatctgcaga tggcgcagat acttctacgc aattaactag atctcaaatc
     2341 caaacattgg ttgataatga tataaaaata gttggtagat atcttactgg tactgttgga
     2401 attggaaaag atgaacgaaa taagtatcta actacagagg aattgaataa tattttttct
     2461 aaaaatttat ctgtgtttcc tatctatcaa gatggaggtg cagcattagc ttattttact
     2521 tatgatcgtg gcttatcaga tggtaagaag gctatagatg cagcaaaaaa tctaaatata
     2581 ccattaacaa cagttattta ttttgctgtt gatctagaca tgttagggga agaaattctt
     2641 gcatatgctg gagaatattt taagggggta tctgctgtaa tgtcatatag cgggtatcaa
     2701 acgggtgttt acggaacaag aaatgtttgt tctcaaataa tcaataacgg gtatgcttcg
     2761 tttgcatttg tttcagatat gtcgactggt tatagtggga atttaggttt tcctatgcct
     2821 agacaatggt cttttgatca atttgttgaa tttaccatag gaagtggcaa cggagctgtt
     2881 ggtatcgatt taatcgctac atcgggtaga gatagcggat ttaatgagtt atcaaatgat
     2941 actaataatg ataattacat tgccaaatac aatcagaagg ttataaatca gatggtacgt
     3001 gcgtatcaat atctatcaca agctggtctt gataatcctt ggaaccctca tttgacattt
     3061 tatagatatg taaattattc tggtttgtca tgggacatca tttcttctcc tgtaacagag
     3121 catgatagaa aaatttatga tgaatatagg aataaattaa ctaatagcga gggcctgtat
     3181 aattatttta tagatcctaa tactggttca ataataggtt tgcctcactt aattgtgacg
     3241 ttacaatcac agatgtttct agccgacaca atcaatgata gtgtttctga ctttgcaggt
     3301 tggttaggag atttaatgac ttgctggggt gaagttaaga aattaggaat acaaatggag
     3361 caaggtgtat ttgcattagt aggtacggct gcaggtggaa catttagtct agaagattta
     3421 taccaagatg ttgacgcggt aaatgtttat accttagcaa aaagaaattc tgattcaaca
     3481 gctttatcgg ctttaaaaac gtactattgt gataagggtg ctattgatag atttaatcaa
     3541 tttgttaaga acagatttgg ttcttcagat aaaatcgctt cagtaacaaa tagtttatta
     3601 gaaaatgacg aaacagttga tatcttaggt gtagggataa tttcattttt cattgataaa
     3661 atagttaata attcacttgg gacagatttt acttatcttg attttattgg agatgagggg
     3721 aaagttgcaa atggattttc tgaaaaaatt attcaatttc aagaaaatag aagtaaactt
     3781 aattaagaat cgtttactat ttaaacagtg tccttttcct ttttaaatat atacgataaa
     3841 ataagtatat gaattgtaaa aggacatgat gattatggaa gaaatagaat ggaataatct
     3901 gactcaaaat aataaaaata attctggatg tggaattatc tttatgtttg ttatagtttt
     3961 gggaatagta gtacttttaa taactgtcaa gatagtagat tactttgcga atactcctga
     4021 agaacaaata cagagaaaac ttcctgagtt tgaaagagta ccaccttatg atccaagaga
     4081 taattcattt caagtaaaaa aattaaaaga gtctccaaat gaaatgtggt ttgaagttac
     4141 agttgaatca agcgacttca aagaaaaaaa gaaagaatac aattatattg taaaggcaaa
     4201 agtatcaaaa agatttattt ttaaccctaa attagaaata tatagtgttg taatagaata
     4261 atagtgttaa aaaaattgta aaagttaaag aagtcaaatt ctgatgtgaa aaataaaata
     4321 taccatggaa gacataaaag aaaaactgga taactagttt atttaatggc agaccgttaa
     4381 ggcctgccat ttttatgtat tttagcggaa ta
//
LOCUS       DACECY010000124         4333 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.131, whole genome shotgun sequence.
ACCESSION   DACECY010000124 DACECY010000000
VERSION     DACECY010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.131"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            129..983
                     /locus_tag="IWO38_002742"
     CDS             129..983
                     /locus_tag="IWO38_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0314756.1"
                     /translation="MEKEKLKILEELRRILNNKNEAIIILNNYFKGGVGKSKLSTMFA
                     YLTDKLNLKVLMIDKDLQATLTKDLAKTFKVELPRVNFYEGLKNGNLASSIIHLTDNL
                     DLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIIIDTVPTPSVYTNNAI
                     VASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFVPYLVDTDSATIKSN
                     LEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVLSMYENVFFEMLERI
                     IQLENEKE"
     gene            1082..1297
                     /locus_tag="IWO38_002743"
     CDS             1082..1297
                     /locus_tag="IWO38_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAQ0314757.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDKALEEYIRKYLPDKL"
     gene            1314..1586
                     /locus_tag="IWO38_002744"
     CDS             1314..1586
                     /locus_tag="IWO38_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0314758.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            1588..2451
                     /locus_tag="IWO38_002745"
     CDS             1588..2451
                     /locus_tag="IWO38_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAQ0314759.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAILEETQGNVIVIDNDTFKQQHPNFDELAKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMLQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGL"
     gene            2892..3209
                     /locus_tag="IWO38_002746"
     CDS             2892..3209
                     /locus_tag="IWO38_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314760.1"
                     /translation="MEKTKKLQLEDFTENEFYGTQEQQYLKAQVREELKEQGFIIDSS
                     FEGDFKTWIGVYARPKDKPTYLDPQNDKEAEEQEQYSINGFKQDFSEWFEWEIKNLKI
                     KEM"
     gene            3743..4240
                     /locus_tag="IWO38_002747"
     CDS             3743..4240
                     /locus_tag="IWO38_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ0314761.1"
                     /translation="MKYIKNVETLEELKKAYKKLALKLHPDCGGNEEEMKILNNEYDE
                     LFSKLKNTHKNKEGETYTKETTETPEQFKDIINKLFNLKMDGVAIEVVGTFIWLTGNT
                     KPYKDDIKALEFRYSPKKYAWYKAPSDYKKRSRKNYDMDTIRGMYGSQKIKEEKEEKK
                     YLQAN"
BASE COUNT         1754 a          689 c          751 g         1139 t
ORIGIN      
        1 aagagggctt ttatatatta atcacaaatc acttatcaca aatcacaagt gattaatcac
       61 aaatcacttg tgatttgtga ttcttaatga tacaatatta ctatacaaaa aaagaatggg
      121 gcgtagttat ggagaaggaa aaactaaaaa tacttgaaga attaagacgt attttaaaca
      181 ataaaaatga agcaattatt atcttgaata attactttaa aggtggtgtt ggaaagtcca
      241 aattatcgac tatgtttgct tacttgacag acaaattgaa tttaaaagtt ttaatgatcg
      301 ataaggactt acaggcaaca ttgacaaaag acttagcaaa aacatttaag gtagaattgc
      361 cacgtgtcaa tttttatgaa ggactgaaaa atggaaactt ggcttcttct attattcatt
      421 tgactgataa tttagacttg attcctggca cgtttgattt gatgttactg ccaaaattaa
      481 ctcgctcatg gacgtttgaa aacgaaagta gattgcttgc tactctttta gcacctttaa
      541 aaagtgacta tgatctcatt attattgata ctgtaccaac gccaagcgtt tatacaaata
      601 atgcaatcgt ggcaagtgat tacgttatga tccctttaca agcagaagaa gaaagtacaa
      661 acaacattca aaactatatt tcctatttga ttgatttaca agaacaattt aaccctggac
      721 tagatatgat cggttttgtt ccttatttag ttgatacgga cagcgcaacg ataaaatcaa
      781 acctggaaga actgtacaag caacataaag aagataactt ggttttccaa aatattatca
      841 agcgaagtaa taaagtaagt acctggtcta aaaacggaat tacagaacac aaaggctatg
      901 acaaaaaagt tttatccatg tatgagaacg tattttttga aatgcttgag cgaattattc
      961 aattagaaaa cgaaaaagaa tagaatcaca aatcacaagt gattaatcac aaatcacaaa
     1021 tcacttgtga tttgtgattg ttgatgataa aataagaata agaagaaata gaaagaagtg
     1081 agtgattgtg ggaaatttag gcgcacaaaa agcaaaacga aatgatacac caatcagtgc
     1141 aaaaaaagat ataatgggag ataagacggt tcgtgttcgt gctgacttgc accatatcat
     1201 aaaaatcgaa acagcaaaga atggcggaaa cgtaaaagaa gttatggata aagccttaga
     1261 agaatatatt cggaaatatt tacctgacaa actttaaaag gagtgaaaat gaaatggcag
     1321 ttacatatga aaaaacattt gaaattgaaa tcattaatga gttatcggca agtgtttata
     1381 atcgagtatt aaactatgtt ttgaaccatg aattaaataa aaatgactct caattattgg
     1441 aagtcaattt attaaaccaa ttaaagcttg caaaacgtgt aaatcttttt gattattctt
     1501 tagaagaatt acaagccgtt catgagtatt ggcggtcaat gaatcgttac tcaaaacaag
     1561 ttttgaataa agagaaagtg gcttaatatg gcaaatatag tcaattttac tgacaaacaa
     1621 tttgagaatc gcttaaatga taatttagaa gaattgattc aaggaaaaaa agcggttgaa
     1681 tcgccaaccg cttttttact tggtgggcaa ccagggtcag ggaaaaccag tttgcgatca
     1741 gcaattcttg aagaaacaca agggaatgtt attgtcattg ataatgatac ctttaaacaa
     1801 cagcacccta attttgatga actagcgaaa ctttatgaga aagacgtagt aaaacacgtc
     1861 accccttatt ctaatcgcat gacagaagcg atcataagcc gtttgagcga tcaaggctat
     1921 aatttagtga ttgaaggtac aggacgaaca acagacgttc ctattcaaac cgcaacaatg
     1981 cttcaagcca aaggttatga aacaaaaatg tatgttatgg cagtacctaa aatcaactca
     2041 tatttaggaa caattgaacg atatgaaact atgtatgcag atgatccaat gacagccagg
     2101 gcaacaccaa aacaagcgca tgatattgtt gtcaaaaact taccgaccaa tttagaaacc
     2161 cttcataaaa cgggcttatt tagcgatata aggctttaca atagagaagg agtgaaactc
     2221 tattcaagct tagaaacgcc ttctattagt ccgaaagaga ccttagaaag agaattaaat
     2281 cgtaaagtat cagggaaaga aattcaaccg actttggaga gaatagagca aaaaatgctt
     2341 caaaatcaac accaagaaac ccctgaattt aaagcaattc aacaaaaaat ggaaagcttg
     2401 cagccaccta caccaccaat acccaaaaca cctaaacttc caggacttta aaccttaaaa
     2461 aaggaaaaga gtagttacca aaaaacggta actactcttt ttttataaaa acatttcctc
     2521 atgttttaaa agctgatcga gaaataaagc acacaacggt attctttctt ttttcagttg
     2581 gtacattttg cagtctgtaa cgtgtcattt actgtttgtt accttaacat atccagtcga
     2641 aaacacaagg gtatataaac gagcaagctc gcccttgtgt tttctttgtg tctatgttgc
     2701 gttctcgtca cagcaaacga cacgaacaca ctggcgaaaa tgaaccaaca aaacgaaaaa
     2761 agaaaggagc gtacactgta atttatttct agcttttaaa acatgaggaa aacagcttca
     2821 cttcctcaaa cgcctaacaa aaatggttgt caaaaaacta aaaatcaaaa aaaagaaaag
     2881 aggaaaagaa aatggaaaaa acaaaaaaat tacaattaga agattttaca gaaaatgagt
     2941 tttatggaac acaagaacag caatatctaa aagctcaagt aagagaagag ctaaaagagc
     3001 aaggctttat catagatagc agttttgagg gcgattttaa aacatggata ggtgtatacg
     3061 caagaccaaa agataaaccg acctacttag accctcaaaa cgataaagaa gcagaagaac
     3121 aagagcaata ttctattaac ggctttaaac aagattttag cgaatggttt gagtgggaaa
     3181 taaaaaattt gaaaattaaa gaaatgtaaa agaaaagcct agcaaaaaaa gctaggtttt
     3241 tcttttacat aaaacaccaa aaagaaaaac ctaagaagga taaaataagt tgttccactt
     3301 gcggtgcagt tgctcctcgt tccaaaccca acttatttct ttgcaaagat atttatattt
     3361 gactaacacc tttcctcgaa tggtaaaagc taaccgttcg ttactctacc tcaaatactt
     3421 tcttttttca gttggtacat tttgcagtct gtaacgtgtc atttactgtt tgttacctta
     3481 acatatccag tcgaaaacac aagggtatat aaacgagcaa gctcgccctt gtgttttctt
     3541 tgtgtctatg ttgcgttctc gccacagcaa acgaaacgaa cacactggcg aaaatgaacc
     3601 acctaaacga aaaaagaaag gagcaagata tgtagaacac tcacagcttt taccattcga
     3661 ggaaaacagc ttcacttcct caaacgccta acaaaaatgg ttgtcaaaaa gccaaaaatc
     3721 aaaaagaaaa ggaatgataa aaatgaaata cattaaaaac gttgaaacat tggaagaatt
     3781 gaaaaaagcc tacaaaaagt tagctttgaa gttacacccc gattgtggcg ggaatgaaga
     3841 agaaatgaaa attttgaaca atgaatatga cgaactgttt agcaaactta aaaacactca
     3901 taagaataaa gaaggtgaaa cttacaccaa agaaacaaca gaaacacccg aacagtttaa
     3961 ggatattatt aataaattgt tcaatctaaa aatggacggc gtagcaatcg aagtagtagg
     4021 aacatttata tggctaacag gtaatacaaa accttataag gacgacataa aagccctaga
     4081 atttcgttat tcaccaaaaa aatacgcatg gtataaagca ccaagcgatt ataaaaaacg
     4141 tagtcgaaaa aattatgata tggacacaat aaggggaatg tatggaagcc aaaaaattaa
     4201 agaagaaaaa gaagaaaaaa aatacttaca agccaattaa ggcttgtaag tttatcagaa
     4261 aggaagaaac cattatgttt tataaaataa gaataattga aactttggaa aaggaatttg
     4321 aaattgaagc aaa
//
LOCUS       DACECY010000125         4157 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.132, whole genome shotgun sequence.
ACCESSION   DACECY010000125 DACECY010000000
VERSION     DACECY010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.132"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            179..775
                     /locus_tag="IWO38_002748"
     CDS             179..775
                     /locus_tag="IWO38_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0314762.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="IWO38_002749"
     CDS             899..2698
                     /locus_tag="IWO38_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ0314763.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="IWO38_002750"
     CDS             complement(2976..3188)
                     /locus_tag="IWO38_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314764.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(4052..>4157)
                     /locus_tag="IWO38_002751"
     CDS             complement(4052..>4157)
                     /locus_tag="IWO38_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0314765.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1228 a          706 c          729 g         1494 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctataatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaatat aatcttaatt gtcaaattaa gttagacaga tcatcaatac
     4081 ttacgtaact caaaaatact tccaaaggtg ttcgataatc cagtgatttt ctaggaatat
     4141 tatttcgttt agatgcg
//
LOCUS       DACECY010000126         4129 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.133, whole genome shotgun sequence.
ACCESSION   DACECY010000126 DACECY010000000
VERSION     DACECY010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.133"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..209)
                     /locus_tag="IWO38_002752"
     CDS             complement(<1..209)
                     /locus_tag="IWO38_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0314766.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSD"
     gene            complement(308..622)
                     /locus_tag="IWO38_002753"
     CDS             complement(308..622)
                     /locus_tag="IWO38_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314767.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            complement(690..1184)
                     /locus_tag="IWO38_002754"
     CDS             complement(690..1184)
                     /locus_tag="IWO38_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314768.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            complement(1204..1491)
                     /locus_tag="IWO38_002755"
     CDS             complement(1204..1491)
                     /locus_tag="IWO38_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314769.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGVFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNK"
     gene            complement(1707..3098)
                     /locus_tag="IWO38_002756"
     CDS             complement(1707..3098)
                     /locus_tag="IWO38_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ0314770.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            complement(3083..3694)
                     /gene="mobC"
                     /locus_tag="IWO38_002757"
     CDS             complement(3083..3694)
                     /gene="mobC"
                     /locus_tag="IWO38_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAQ0314771.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            complement(3698..3895)
                     /locus_tag="IWO38_002758"
     CDS             complement(3698..3895)
                     /locus_tag="IWO38_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314772.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            complement(3886..>4129)
                     /locus_tag="IWO38_002759"
     CDS             complement(3886..>4129)
                     /locus_tag="IWO38_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314773.1"
                     /translation="EMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
BASE COUNT         1197 a          775 c          546 g         1611 t
ORIGIN      
        1 aaatcagata cataagccca tgtacccgtt cgttgtaaat aaagttcttc ttgtaacaac
       61 tcccattttc tcttagaaat cataatgacg ccatcgttca atccattttt agaatcccta
      121 gtaatttcta tttcagaatt tgaatgaact acttcttgca acactttacc tatattttct
      181 ttgaagtttg ttggagtaat agtatccaaa atgaatacct ctcttctcta tacctctatt
      241 atgaagtgcc cataaaataa agcaagcgtt gaggaataat tctatcaaca cttgctttat
      301 taagctttca ttttgtagtt tgactaaaac caataatctt atttctaaac tctcgaaaaa
      361 cttcttgtgg ttgataatag tctggatcta tttcaaatgt ttgtgagaat aattcattga
      421 atttttctac tgaaataatg attttaccat tcttctctcg actgttttct attatttctg
      481 cttctaattc aaataaatct agtgacgaac taaaatcata aatcatgtca tattgaataa
      541 aaaaatcaag aaaaagttgc tcaaactctt ccttatttat cactaatgtt tcaaacggaa
      601 caactttatt ttcctcagcc attctcccag ttccattttc aaaaatcaca aaactgatat
      661 taccttaaaa actactaaca aaataaaact tataaagtaa atggcaaccc aactatgtaa
      721 ccattctctc tacgtactac gatttggtag tcctgccttt caatcggtaa ttcataatag
      781 taatttactg tttcttcttt gccactttca atatactctg gattatccaa tatcatatca
      841 tactcataaa gttcttcacc atccttattt tcaatcgacc attgttgtgg acgtatcgga
      901 ttttctgtat catttataag tgtataggtc acttgtaata cacgatcatg ttgtgcaaat
      961 tcatttcttt tttcggtcac ttcaatattt ttaaatgtta gttcaacccc atctgcctga
     1021 taagtttcac ctatttttaa atcttttgtt acaattttta ttggcttttc ttctggaaaa
     1081 tgtctggctt taattgttag ataacttcca aaaagaatca atcctagtag cactaaaaga
     1141 attcctttaa cgagccactg aaacgggaag taaactttaa acatcttttt tctcctccgt
     1201 tttttatttg ttttttagag aatacgataa cttttgatca atgtcatcca gcttttcaat
     1261 tacttcatta cttatcattg attctagttc tgaaattttt tgttcaaatt taatgctttc
     1321 aggatcatta aatttattat ttgatggact agctaacact ggtaaattcg aaaccataac
     1381 acgaaacata tttgcctttg aaccaaaaac tcctttgttt tggttgtaaa aattgttgaa
     1441 tatttcttta gtttcttctg aagccgagac tttaaaatat ggatagttca tttttttctt
     1501 ccacctttct ttttttggga gggagtctgt agggagcttg ctccctcaga gatttaccca
     1561 ccatctgact tgccagaaag tagcctagcg tgagcggggc ggattgactg gcaagtagat
     1621 gggtagctgg cgtatgtcct aaaaactgct tctttccaag caactcttca taggcaaaaa
     1681 actggaaagt ccactaacaa gcccattcag aattccattt caaaattttg ttgccttttt
     1741 tgctgatttt caatcatttg ttgtgtgaat tcataatcct ttttagcacg atacattttt
     1801 acatcgtcat tcattatgcg actgatttga tagtgaatct gtttagcaga aggtgtatga
     1861 gaacaatttt caaaaccctt tgccggtaga aataactctt tctgatcagt ctttttaata
     1921 gaaattgttt tacttttcgc actacgtaaa gtttgaggat tcacatccgt atgaagttta
     1981 atcgtgtatt cttgatcatc ttctaaagac attgtattta caaaatgctt gtctacctct
     2041 cgcaagaagt catcatattt aatgaatttt tcatcgcctt cacggtcgct aaattgcttt
     2101 tgtgcgatgg aatctaattc cacaaacggt acatttaact caccagtttt gtaatttact
     2161 ccactaatta gcgacataac ttgctctctt tccatattaa aaaaattttt tatatgtgat
     2221 tcttctaaat tcttttcaag ttgttcagaa acagattcaa atgaatatct tttccctaac
     2281 tttcttgcac taaaagtacg ttcgacatac tgactttttt cttttctaga ttgtttatct
     2341 cttcctaaag taattaactc acctctacgc tcatagaaat ttacaccttg tttgtaaaga
     2401 acatagccaa gtttgttatt tgtttttgaa aattgataaa cttcaatgtt atattctttt
     2461 gccaaatgat ccgaaaaatc ttcaaaagaa ttcacttcgc atttttccaa cgttgcttcg
     2521 atcgcatttt gcacaaactc ttgataatta atttttccat atttcatctt agaaggatcg
     2581 tttgaataaa gattggttct cttttctaag ttttcatagt actcattatt cactgattca
     2641 agaccatgtt cattgaggac ttgatcgtta atttttcgca ttagttctaa atctttaggg
     2701 tcaatacgca accttttaca tgtttccatg ttaatattat taatgacaag atgactatga
     2761 aaatgatctt tatcattgtg acttgcaaca atgatttgcg aatttggaaa agctttctct
     2821 gcaaattcga cagctatttc gtgcatttta tcaatactta actctttaga agtactctta
     2881 tcaaacgaat gaataacatg aaacgcttgt gctatatctt tagatttatt catcttggta
     2941 cgtactaaat tcatttcttg cagtgcttcc tcactatcat cactacaatt tattcctgag
     3001 actgcaacac atttttcttt ttttgaagga ttagctgcat attgtaatag cgaggataaa
     3061 tttttcttgc gtcctttcga tgttatagta gctcccaaag ttgtttcacc tcatctttta
     3121 aacttgcaat aatctgaatt gagtgaatta aattccgatc aactttttgg gaatactctt
     3181 ctttcgtacc cggtctagtt tgcttttcta tccaatattc gaaatattct ttttctactt
     3241 ctgataattt aaaaaaagag ggggcactcc gattggactc caaaaaaatg tcatctggat
     3301 aactaatcat caactttttt gctatgctat tcgcaagttc atctttaaaa atgttgtaac
     3361 ggctagcatc aaaactaaaa tgtaggctct tttctttttt atcttcagat tcttctctaa
     3421 aatatttagc aatgatattt aatgccttta ccgcttgatt caaattgtta ccaatttgat
     3481 ttaactgttt ggttaaaact accctatcat cttcagctaa caacggcggt tcatatttga
     3541 gtccctttaa ccgctcacgg caaacgtctg ctctagaaat tcccctttgt tttgcaatca
     3601 atgtcagttc aagataagtc tttttatcga cacgaaaatt taaggcctca ctatgtttgc
     3661 gatttttttc gtacattttt tgtttatcat ccattgatta tcccctcaca tttttttcta
     3721 gagagcaaca tttgaaattg ctctctattt catttttcat ctttaattct tcttgaatca
     3781 tcagttcaat ataattagtg accgtgattt tttcatttgt tgcgttcttt actgattcac
     3841 ataattcgtc aagttctaag aaatcatgcg tactaatctt tagaattaat tgcatgacaa
     3901 tccccccctt ttatttctta ataaattatc gaaaagtgga ttaggatcat taagtgtatc
     3961 tctgaataac tcattgtctt cataagtgaa tcttcgtctt gattttgatc ccttatcatt
     4021 ttcacggatt tcactatcaa taatcactgt ttcatgattt gaatcaaatt ttttcgctga
     4081 ctttctgtct tcaacgatat tgattttttg tgttttttct ttcatttct
//
LOCUS       DACECY010000127         3956 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.134, whole genome shotgun sequence.
ACCESSION   DACECY010000127 DACECY010000000
VERSION     DACECY010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.134"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..156
                     /locus_tag="IWO38_002760"
     CDS             <1..156
                     /locus_tag="IWO38_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAQ0314774.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="IWO38_002761"
     CDS             175..1452
                     /locus_tag="IWO38_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0314775.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="IWO38_002762"
     CDS             1462..2118
                     /locus_tag="IWO38_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0314776.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2118..3494
                     /locus_tag="IWO38_002763"
     CDS             2118..3494
                     /locus_tag="IWO38_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0314777.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3624..>3956
                     /locus_tag="IWO38_002764"
     CDS             3624..>3956
                     /locus_tag="IWO38_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0314778.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1594 a          423 c          693 g         1246 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaatta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
     2161 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
     2221 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
     2281 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
     2341 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
     2401 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     2461 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
     2521 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     2581 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     2641 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     2701 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
     2761 tagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
     2821 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
     2881 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
     2941 gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa tctatatcag
     3001 gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca ggagttgtag
     3061 tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca gtattactat
     3121 cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg gtatttatat
     3181 caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac aagcttggga
     3241 ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa caatctgcat
     3301 ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta tcaagtttag
     3361 atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt ttagtacttc
     3421 ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa gaattattaa
     3481 ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aatgagagtg taatataaac
     3541 cgtgtaagta accatcaatc ctaggcgggt ggttgcttca cggtttttat ttttctaaaa
     3601 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gctgagaagt
     3661 tagctgaatc cattttgaat acttatcaac ccgaatctgt agaggatatg caagatgttt
     3721 taaaagatgt gtttggtccc ttatttgaaa aaatgcttca aggagaattg aataaccatt
     3781 tagggtatga cgcccattct aaagagccaa agaaacatga caatcgacga aacggttatg
     3841 gcaataagac gcttaaaacg agctttggta aagtaaacat tgatgttccc agagaccggg
     3901 aggcttcttt tgaacctgag ttgattccta atagaaaacg agatgtctcc gacatc
//
LOCUS       DACECY010000128         3900 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.135, whole genome shotgun sequence.
ACCESSION   DACECY010000128 DACECY010000000
VERSION     DACECY010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.135"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..580
                     /locus_tag="IWO38_002765"
     CDS             <1..580
                     /locus_tag="IWO38_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314779.1"
                     /translation="EESTTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTG
                     NGTGSSTPAPTPTPTPEQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSG
                     FDCSGFTRYVYMQVTGRDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIA
                     LGGGQYIHAPQPGESVKVGSVQWFAPDFAVSM"
     gene            complement(648..2447)
                     /locus_tag="IWO38_002766"
     CDS             complement(648..2447)
                     /locus_tag="IWO38_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ0314780.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            2570..3076
                     /locus_tag="IWO38_002767"
     CDS             2570..3076
                     /locus_tag="IWO38_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0314781.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            3155..3877
                     /locus_tag="IWO38_002768"
     CDS             3155..3877
                     /locus_tag="IWO38_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0314782.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
BASE COUNT         1179 a          732 c          821 g         1168 t
ORIGIN      
        1 agaagaatca acaacagtgc ctgaatcttc agcaacagaa gaatcaacaa cagtgcctga
       61 atcttcaaca acagaagaat caacaacacc tgcgcctaca acaccatcaa cagatcaaag
      121 tgttgataca ggaaacggca caggaagtag tactccggct ccaacgccaa caccaacacc
      181 tgaacaacca aaacctgtaa cacctgctcc agctccttct ggaagtgtga atggtgcagc
      241 aatcgtagca gaagcttaca aatatattgg tactccatat gtttggggcg gtaaagatcc
      301 aagtggcttt gactgctcag ggttcacacg ctatgtttac atgcaagtaa ctggtcgtga
      361 catcggtggt tggacagttc ctcaagaatc agctggaaca aaaatttcag tttctcaagc
      421 aaaagctgga gacttgttat tctgggggtc acaaggcgga acttaccacg tagcgattgc
      481 tttaggcgga ggacaatata tccatgctcc tcaaccaggc gaaagcgtaa aagttggttc
      541 agttcaatgg tttgcccctg actttgctgt cagcatgtaa gaatgattaa tcaaaaagag
      601 ttagcccatt ctggactaac tcttttttta ttgtgataaa atagaaatca gataaattct
      661 tctgtcaatt ccatgaacgt ttggatatct tcagtcacca tgtcgatacc tgcttgccaa
      721 aaatctggtt gggtaagatc gacattcaaa tgtttcttcg caagatcttc cgtcgtcatt
      781 gaggcagtat cgcgtagtaa atggatatat tcttcttcaa aatttgctcc cttttggttg
      841 gcgtaagcat agatgcccaa actaaagagg tagccaaatg tgtatgggaa gttataaaac
      901 ggtacgtcat caataaagaa atgcaatttt gcagcccaga aatatggatg gtaagtagct
      961 aaagctcctt gatagccttc tttttgcgcc tcaaccatca tctctgtgat cttatcttct
     1021 gagactaatc cttcttgccg tgcttcatag aatcgatttt caaaaatgaa tcgggaatgg
     1081 atattcatga acatagctaa agcattttgc agttttgtat ctagcaaatt gattttttct
     1141 tcttttgttt ttgcgacttt taatgtagca tcggctacga tcagttctgc gaatgtactt
     1201 gctgtttctg cgacattcat ggcatattcg cgatttaaag aaggtaaatc ccacatcgtg
     1261 ctgctatgga aagcatgtcc tagttcatga gcaagtgtcg caacctcatt aacagaatta
     1321 ctgtaggtca taaagatcct tgattctttt gtttctggta actcagtaca ataaccacca
     1381 ggacgtttcc caggacgatc ttctgcttcg atccagctct tttcaaaagc agattgggca
     1441 aatttagcca tttttggact gaatttgtta aagttttcga taataaatgc tgctgcttcg
     1501 tcaaaactaa aggttttttc tttcaaatcg cctaaaataa taggtgcatc ttgatcttgc
     1561 cattccattt tttctttacc gaaaagatta gcttttctag tcaagtaatt aacaaggggt
     1621 tgtttgtttt tctggattgt atcccacatg acagacaatg tttctttctt caaccgattg
     1681 tactctaatg gtttttgtag aaaatctgtg acaccatgaa gttcatagtc agaaagtcta
     1741 aaaccatcta aatgattcaa agtatccgtg aagaggggtg ccttttcttt ccaggcttct
     1801 tcccatgctg cgaaaagtgt ttctctgact tttggatctg gatcgcccat catcttattg
     1861 aaggcttgtc cagcagataa ttcgactact tgtccatctt gttcgaaggg aatcgaaatg
     1921 ctggctacaa tcgtatcata atgactgctc caagcattta gaccatctaa agaaagcgta
     1981 tttataatgt tttcttcagc ttctgataat aattgtgagc catcacgacg aatctcgttt
     2041 aaacgaaaag caattgtttc aaacgaagat tgagaaagca tctgattcca atcattgtca
     2101 gatatttctg caaatttctt tgacaataca gtttcggctg attgcagacg aggcagcatt
     2161 gcgtaaagct tgcctgataa gacctttgca tctgaatcat tgacattggc agaaagcaat
     2221 gcagtaatat agctgctgca ttgggtaaaa ccattcgtaa tcgtttcttg caattgaaga
     2281 atagcgttta actgctctgc attgtcagaa ctaggtgacc attttgttac gcggtgataa
     2341 tactcgttca tttggtcttc caattgcttc atccgttggt tcaaagcatc agaatgactg
     2401 cctccggaaa aaatagaatc tagattccaa tttaatgaat aactcatgaa aaaactccct
     2461 tcatattgta atacaaaatt aagtatagca cttttttatg gaagaagtct ctatgaaaat
     2521 gattgttgtt tcttcaaaaa aagtctaaga taaaatacag aggtgactta tgaattcttc
     2581 aaaaaaacaa atgaaaaacg gaaatattta tttagtaatc gcactgatag tcgttataat
     2641 cttattttac agttcttctc aaacttacgg acagcaatca caagtttcgc gattagaaag
     2701 ctggctgcct aatcagccat ttaaagacac attagcgggt atacgtttta attatggtgg
     2761 aagtatggtg agtatcgacc atctgggcta cgccaaattt ttagagttct ttttaaggaa
     2821 gggcgcacat ttcggtacct attttatttt gggaggcagc ttgtttttag gtgttttccc
     2881 caagctaaaa atttggtggc tgacagcgat ccttgcttgg ctttcagcaa cagggtacgc
     2941 aggtttggac gaattccatc agatgatgac cggtggacga acacctatgt tccaagatgt
     3001 catgttggat tcgatcggtg ctctgactgc tgtgatatta tgtgtgcttt ttgcggcatt
     3061 caagaagcgt ttatagattg gctgtggctt tgtttttttg tgaaatagta ctagaataga
     3121 agtgtattct caaaagaaca agggaggaaa aatcatgtca ggacatagta aatggcataa
     3181 tatccaagga agaaaaaatg cccaagatgc aaaacgaggg aaagtctttc agaaactttc
     3241 tcgtgaaatt tatatggcag ctaaagcagg cggtcctgat ccatctatga atccagcttt
     3301 gcggcttgtt gtggacaaag caaaagctgc aaatatgcct aacgacaatg tggaacgggc
     3361 gattaaaaag gctacgagtg ccgtagatga aacgaattac gatgaaatca cgtatgaagg
     3421 atatggtccg ggtggagtag gaatcttagt ccatgcattg acagataacc gtaatcggac
     3481 agcgacaaat gtccgagtag cttttactcg aaacggtgga tcactgggag aaacgggttc
     3541 agtcagttac ttgtttgacc gcaaaggata tatcgctatt gaacgcgatg ggttgactgt
     3601 tgatgaagag acgatgtttg aggatgtatt agaggcgggg gcagaagatc tgcaaacttc
     3661 tcctgaagta ttcgagattt atactgcacc agaagatttt gctgctgtac gagatgagtt
     3721 ggaaaaagag ttcactttgg cgcaagcaga attgacgatg attccacaaa cgacgattga
     3781 tttaaatgag gaacaaaaag agcaattgga acgtctagta gataagctag aagacgatga
     3841 tgatgtttca gaagtcttta ctgctgctga aatgtaagta gcacattgtt agataataaa
//
LOCUS       DACECY010000129         3895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.136, whole genome shotgun sequence.
ACCESSION   DACECY010000129 DACECY010000000
VERSION     DACECY010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.136"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(92..1177)
                     /gene="xerS"
                     /locus_tag="IWO38_002769"
     CDS             complement(92..1177)
                     /gene="xerS"
                     /locus_tag="IWO38_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ0314783.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3253..3771)
                     /locus_tag="IWO38_002770"
     CDS             complement(3253..3771)
                     /locus_tag="IWO38_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ0314784.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1258 a          635 c          589 g         1413 t
ORIGIN      
        1 atataaaaat gataatgaag cttgccttta aagttgggct tttatctaat tttagagaca
       61 ggcttcatta ttatactatt ttttactaca tttacaaagc gttcaaagca tcaattgtag
      121 ctgcattgtc tatatgagta tataaagttg ttgtatttgt acttgtatgt cccaattgtt
      181 gggcagttaa taccaatgaa tttgtctgtt gatagagtct agtagccaaa gtatgacgta
      241 atttatgtgg actgacttgg actctgaatc cttttgaata ttttgctacc atccgttcga
      301 ttgcaatacc ggatatacgc ttggcttctc cttgataaat tgctagaaat aaagcttttt
      361 cgttttctgt tactttgtat cgttctttcc gaatagctag ataattagct aaatattcca
      421 ttgcaaatgc tgctatattt actgcgtctc tcttatttcc ttttctagtt actaccacat
      481 tattcttttc taaagatagg tcctctaaat taatatttac aacctcggag agtcttaatc
      541 cagatcctaa aatcaatgca ttgatcgcca ggtctctttc tacatctcgt tgataataga
      601 tcaaagctcg tttgctcaaa gtttttttat attcattggc aatataatct agataatttt
      661 gtgtatcttc gtctagaaat aattttttct cgattgcgtt tgcacgggca gctaacgtag
      721 ttttatctgt gatagttgct acttgatcca taggattatt ttctaagtat ggttttccta
      781 aaggattatt ggaacttttc gaaagatatt taaatagaac ctttaatgct gtgatatttc
      841 tattgatagt agaagtagat agacctaagt tattattatc tttgagctta gtttttgttg
      901 tctctttttt tacattttcc tttgtgcgac gtttcaaatg agatttgtac aagtttagat
      961 cataggcagt taactgtctt atcgaggtga ttgaagtatc gggaacgata gactcactca
     1021 gaagccattc ataaaagagc ttataatttt ttagatattc taaagtagtt cgaggtgatt
     1081 gatctttatc ctcacgatct tgaacaaaat cttttatata atcgggcata tgaagtatga
     1141 ttgtatctat tttttgatat atttctgtat ttttcatgtt attcatcctt tcgataagta
     1201 ggggagagga gtatctctat aattggacta taccaattat agagtgtttt gtcaattggt
     1261 atactctgaa atttattaat ttatttcaaa aaagcagata aataggtata gatttaataa
     1321 tttaaaataa atggtataca tgaataggaa aggaaacaga aggaggcaat cttaattttt
     1381 taaattgtct aaattatata gaaagaacaa tctttctata taattagaca taagagagac
     1441 tacggttgat ttgatattaa caagcgtata tgaatgtata tacaaataaa aaaggggagg
     1501 gagaagttaa agaaaattca gagaagtggg gcactcagta gtattaacaa tttaaaagga
     1561 atttcttaaa gaaaatgggt tgcatcctgg agatacaatt tttttagata aagaaaaaat
     1621 tcaagcaaca attcaaaaaa atcttaactg ctgagattga tgtagcaata tctcaatcat
     1681 caacaaagta cgatcctgcc tttcaaaagc ttattgataa atgtttattc attataaaaa
     1741 taaaagaaaa atttatgtca gcttttttca tcaaagtggt aatgataatt cgtctattaa
     1801 ttgtattttt taggaaatat ggtataattg ttttaacgaa aaggtaattc atgatacacc
     1861 aaaaaccact agctggcggg ctagtggttt tcttttacgt tctattcaga ttaacgggta
     1921 ggcttactgt cttttttggc aataattttg caatcaagcc atttacagat gtaatgcgcg
     1981 gatacttctg ctaagattgc aaaaaacata tcgaaaaggt aattcataat acaacctcct
     2041 ccccttgcca taataggccg gggtagcaga cagcataaat attataccat aactgaaaat
     2101 ttcattttag tacatttcaa tatattgatg aaccagcaca ataaaacatg ccatgactaa
     2161 cagaaaaaaa cttattaaac cagatataac ttgtaaacat ccaactatat agaaaacgta
     2221 aattttctat atagttggat taagataacg actatttatt ttatcatatt tttactatac
     2281 cctatacttt tacttggtat atttatgaat aatcgcaaat ttacccctat actactagtt
     2341 tgtaagttag attaataaaa atactaaagt tatttgttct tttattaaat acacctacaa
     2401 cttataaatt gatggtattt agataacacg aaagaataaa aataacagct gatagtaaag
     2461 tttgttgacg attgctactc aatcgaaagt gagttagtgc gtatgaactt gcttctgaaa
     2521 acttatgaaa tgaaagacct tttacatctt gcagaaatcg gtactgggat taaactaagc
     2581 aataaaaact aatgttcccc tcattctttg aacttctact atttgattta tttattctga
     2641 taaatgacga tttcaccttc aggatcttca aatcccgtat agaatttatt taagatctct
     2701 tcagtatcag taataggata ggcgataggc cacataccct ttgatggtta gaataaactg
     2761 ctcaaaatct gtatcgtatg ttggctttag catattaaca aaggctatct ccaaattata
     2821 aaccataagc gttgaatcgt tcttaacaat ttcaattctt cctaacccaa agtcagaaga
     2881 tataattacc ggacttgcct tacccttcat gtgggttgtt gagattccat atttaaaggg
     2941 aattgtcggt actatcggcg gtctgaaaaa catcccaaaa ataaaaaaat ctttcccctg
     3001 ctagtctcct aagtcccttt gtagcggata tggcaccata atctcatttt atcgaagtga
     3061 gtcatggttt atctgttggc caaccaaaat tgatacaatc taacgatgca ccctctacgg
     3121 cattctaggg gagtgcactt gcattcaccc aataaaaacc ggcagttagc aaagaactta
     3181 tgtagtcgta tgttcttact gttaacatac aagaggtctc ctttccacaa ttgcttagca
     3241 gtaaacggga atttacaaag ctaatctctt ccattttctt atctttgttc taaaagtccc
     3301 aaatggagca actgtattta catgaataaa tttatatact tcccatacag ccgttttggt
     3361 tgcctcgtct gcccattttc ttttgtgtgc agtgaataga tcttcatcac tcattgaatc
     3421 aatcatcatg ttaatatcat caatattacg attgagaatt tcctctaatt ctttcaatgg
     3481 tagatgtgca taggtatttg taaaccactt atataactct cctaactgat tccatttaaa
     3541 ttgatcagaa ggcgttttaa cagataatcc atttctttca tcattttccc attttagaat
     3601 taaattcgtc caaccaactt gatatgctaa attttcagca ggtgttctat ccacttcctc
     3661 aactctttta tccttcaatt cttcaggaat agtttcaaat tctaaaatat atttagtata
     3721 agtcctattt atttcttttt ttaattcttc tttattttta tatgttctca ttatattacc
     3781 tccataaatt cctaattgtc tcctttaatt catctataat ttcatccacg atattttaat
     3841 ccgttctaca atgcatatct gatatgcatt gactgtctaa ctcatatgtg aatga
//
LOCUS       DACECY010000130         3785 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.137, whole genome shotgun sequence.
ACCESSION   DACECY010000130 DACECY010000000
VERSION     DACECY010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.137"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..171
                     /locus_tag="IWO38_002771"
     CDS             <1..171
                     /locus_tag="IWO38_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0314785.1"
                     /translation="SLFPLYHGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFK
                     IEYTKKRQRLAY"
     gene            346..642
                     /locus_tag="IWO38_002772"
     CDS             346..642
                     /locus_tag="IWO38_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314786.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            649..2601
                     /locus_tag="IWO38_002773"
     CDS             649..2601
                     /locus_tag="IWO38_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ0314787.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="IWO38_002774"
     CDS             complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="IWO38_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ0314788.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3400..>3785
                     /locus_tag="IWO38_002775"
     CDS             3400..>3785
                     /locus_tag="IWO38_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ0314789.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRKKIENPHPLLQTTVE
                     PSKPEQREMLLDALLEISDSDPLLRY"
BASE COUNT         1344 a          614 c          655 g         1172 t
ORIGIN      
        1 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
       61 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
      121 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag
      181 ttttggagta caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt
      241 gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag
      301 ttaaaatgta tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg
      361 ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag
      421 ccaaaaaata agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata
      481 gttaagcaat acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta
      541 ttgaacattt tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag
      601 cggattgttg aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt
      661 aaaagattag ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa
      721 gaattaaaag atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg
      781 tataacaaaa tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt
      841 caatataatt actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc
      901 aaagatgtaa aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt
      961 cataaaggta tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca
     1021 gtaacccctt tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat
     1081 agcatccaag atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa
     1141 tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct
     1201 caacgctatc aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa
     1261 accactactt atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt
     1321 ggaagggttt cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat
     1381 cataagctgg agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa
     1441 ccccttcaaa caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac
     1501 ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt
     1561 aatatgcaaa cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct
     1621 gatgttgcct atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc
     1681 aaagaagacc atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat
     1741 ccacaagaac catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg
     1801 aaaattacta ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca
     1861 actgctcact attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt
     1921 gatgatgact tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg
     1981 agcgatgcac atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta
     2041 aaagaattcg gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa
     2101 acaaagtttc tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa
     2161 ttccacgaag aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct
     2221 atcaaacatc cattagccac taaggataaa ggattttatt cagttgattg tagaacggat
     2281 ttatctgctc tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg
     2341 accaatagct ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca
     2401 acagctcgtg gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa
     2461 tttgcgatta caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat
     2521 aaaaaggttt tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa
     2581 gatataattt atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta
     2641 tctttactgt tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat
     2701 acatgaaaat agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc
     2761 tcattccatt cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt
     2821 atttttgtga tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct
     2881 ccgaaaataa aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga
     2941 acaatttcac aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg
     3001 tcaggtaagg ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc
     3061 tttgtagtat ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct
     3121 ttgccaatca ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca
     3181 gcctttttaa ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta
     3241 tcctgcccgt tagttgaata agcaattttt tctataagtt atattatcat aataccacaa
     3301 aatcacaaat tttcattcaa ttatcatttt caattgcatt ttataaatca ttttgtatgt
     3361 aatttggtat gtaagttaaa atgaaaaaag tagtccattt tgaagtacat tttgtcattt
     3421 tgtactccaa aactgaacta cttacttgca tatatacgcc tttatagtgg agtactacat
     3481 ttacgagatt cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact
     3541 tcaataaatg gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg
     3601 caaaatgagt ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga
     3661 aaaaagattg aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa
     3721 cagagagaaa tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga
     3781 tatta
//
LOCUS       DACECY010000131         3741 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.138, whole genome shotgun sequence.
ACCESSION   DACECY010000131 DACECY010000000
VERSION     DACECY010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.138"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..437)
                     /locus_tag="IWO38_002776"
     CDS             complement(<1..437)
                     /locus_tag="IWO38_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0314790.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAVRIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVY"
     gene            751..1173
                     /locus_tag="IWO38_002777"
     CDS             751..1173
                     /locus_tag="IWO38_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314791.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1183..1386
                     /locus_tag="IWO38_002778"
     CDS             1183..1386
                     /locus_tag="IWO38_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314792.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            1597..2181
                     /locus_tag="IWO38_002779"
     CDS             1597..2181
                     /locus_tag="IWO38_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0314793.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            2552..>3741
                     /locus_tag="IWO38_002780"
     CDS             2552..>3741
                     /locus_tag="IWO38_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ0314794.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PVTDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKI"
BASE COUNT         1279 a          583 c          705 g         1174 t
ORIGIN      
        1 ttataaactc catttgcttt cgcaatttta atgccttgcg cctggcgtcg tttagattcg
       61 gtacgctctt gttcagcgat catggccaaa atttggatga tcagatcctt aatgaattta
      121 tccaggagag ggttgccaat tgcttcagcc atgatcggta gactggtgat gattagctgt
      181 acgtttttct tctttaggta attgactgaa gcgatgatct cgtcgtaatt tcttccaagt
      241 cgatcaatag cttccacaat aaaaatgtct tgatctcgga cgaaatccaa tgcttcttgg
      301 aaaacaggtc gatgagtaat cgttgcgccc gattgtttct cgatgaaaat tctcactgca
      361 ccgaattttt tcatttcttc aatctggcgt tgttcatttt gatcgatgga tgagacacgt
      421 atgtatgcaa ctctcaaaat ttttacctac tttctgcaac tatttaaaat acaccctaat
      481 catacacgat aaaaccagat ttttcaatgt tgtggtcgtg tctagttagg gtatacctta
      541 tatagacaaa aactaatgat ggaggaaata gtatgtgtca cttttctttt taaatggcaa
      601 tattttttgt aaataaatgt caatcaagaa aatttaaacg attatgacat taaaaatata
      661 ttttatgaat tttctagtat ataacttgat ttatctgtta agatttatta agaaaacgtt
      721 ttcttatttt tgatttcgag gtgatagaca atgaaaaata agtttatttt atctttaggt
      781 attttaacta ttggaattag tgtaactgga actacaattc ctacatttgc tgatagtaca
      841 aatgaaccaa ctactgtagt agataataac caagaattaa ctcctttaga taggaacgga
      901 atagccattg gtaacccaac taacgactta actgttactg aaaattattt aatagtttct
      961 ccattacttg ctcgagcagc ttctaaaacg atacgagtga caggaacgat gatttttgat
     1021 aaagttggtt ggccttctag acctcctcaa tcacggaatt ggaaagaaaa aagaaatggt
     1081 tattggtact atggaaaatt gtatttacaa agttataaat cagctggaaa caattggatt
     1141 gcgacttatt caggaacctt aactagaaag tagggatttt ttatgccaga atttgtgaac
     1201 acaccaaatt tttatcaaga tagcgatatt ctaagtaaaa ttaatttacc gaaagaagaa
     1261 tcagctttac aagttcagcg aactttacag gagacacaaa aaattcaaaa atcaatttct
     1321 aatctagaag attctgaaga aaaaagaaaa aaaagagacc aaaaaacata tcaaaaagaa
     1381 aaataatgat tttaaaaaaa gagtgtctag tcgacattct ttttttctaa ttgaaagtgt
     1441 tcataaaatc acccatttct ggacacttcc tttttttgct ttatttttca aaaaaacaat
     1501 tcatttcatg ttcataaacc cataacaata tctatgttca gttaaactat atggataaag
     1561 ttatgttaca ataaaattaa tgaaggagtg atttttttga aaataggata cgcccgggta
     1621 tctactggtt tacaaaattt ggatttacaa gaagacagcc taaataaatt tggttgtgaa
     1681 aaaattttta ctgatcatat gagtggggca aaaagtaatc gaccaggatt agaaatggcc
     1741 attgattttg ttcgctctgg cgatacactt gtcgtttggc gcttagatcg tttaggtaga
     1801 aatatggaag atttgatttc aatcgtcaat cgactgaatg agcgtggtgt gagttttcat
     1861 agtattcaag aaaatattac gatggacaaa tcaagttcta caggacagct catgtttcat
     1921 ttgtttgcgg catttgcaga atttgagcgt aatttgatcc tggaacgatc cgcagcagga
     1981 cgagcagctg ctcgtgcaag aggacggttt ggtggtcgtc ctgaaaaatt gtctgaacaa
     2041 gatttggaat tactgaagac tttagtggat agcggtacac ctattaagac tattgcggaa
     2101 cggtggaacg tctcaagaac caccatttat cgttatttgg caaaaatgga ggagaaaaat
     2161 ggaaagacaa attctcatta gtttttgtca tttaatgctg aaaatttttg ccatttatct
     2221 agaaaagtga caatagtatg agaatttttt gtaagtgtct actgataact tattatcaaa
     2281 tgttgtaagg attttattca tttgttaata ttaaagaacg gaactccaaa aaagtccgta
     2341 ctctaatttg cagtctctca ctcttttaga gtgagagttt ttattagatt ttcaaattaa
     2401 ttagtaaagt gatagaataa attgatcata caaatcataa gaagaagcag ggtgatagca
     2461 tacctatgct tcttttttta tgtatttagc tatttcttct ttacaacatc gaacatatgt
     2521 ttgtataatg tggaagaatg gagtgttatt tatgatggat tattcaaaag agccagtaaa
     2581 tgattacttt ttaatcgata tgaaaagctt ttacgcaagt gtcgaatgta tagaaagaaa
     2641 tttagatcca ttaacaacag aacttgttgt tatgagtcga tctgataata ctggttcagg
     2701 attgatatta gcttcttctc ctgaagcaaa aaagcggtat ggtattacaa atgtgagtag
     2761 accacgtgac ttaccacaac catttcctaa aacactacac gttgttccac cacgtatgaa
     2821 cctatatatc aagcgaaata tgcaggtaaa taatattttc agaagatatg tggctgatga
     2881 agatctactg atttactcga tcgatgaatc gattttaaaa gtgacaaaat cactgaatct
     2941 ttttacgact gaagaaacac gaagtcagcg aaggaagaag ctcgctcaaa tgatccaaga
     3001 acgtattaaa gaagagttag gtttgattgc tacagtaggt gtcggagata atcccctttt
     3061 ggctaaacta gccttagaca acgaagcaaa gcataatgaa ggatttgttg cggaatggac
     3121 gtacgaaaac gttcctgaaa aagtatggaa tattcctgaa atgacggact tttggggaat
     3181 tggctctcga atgaaaaaaa gactcaatca gatggggatt atgagcatta gagatcttgc
     3241 taactggaat ccttatacga ttaagaatag actaggtgtg attggcttac agctgtactt
     3301 ccatgctaac gggatcgatc gaacagatat agcgatccca ccagaaccaa ccaaagagaa
     3361 atcctatggt aattcacaag ttttgccacg agactatact aggagaaatg aaatcgaact
     3421 agtagtaaaa gaaatggcgg aacaagtagc cattcgtatc agacagcata attgtaaaac
     3481 aggatgtgtc catttacaca ttggcacgtc gatcttagaa acgagaccag gattctcgca
     3541 ccaaatgaaa atacctgtca cagacaatac aaaggaattg cagaattatt gtcttttctt
     3601 atttgataag tattatgaag gacaagaagt aaggcacgtc ggaataacct atagcaagct
     3661 tgtgtacacc gattcgcttc agctggattt atttagtgat ccgcaaaaac aaatcgatga
     3721 agaaaacctg gataagatta t
//
LOCUS       DACECY010000132         3716 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.139, whole genome shotgun sequence.
ACCESSION   DACECY010000132 DACECY010000000
VERSION     DACECY010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.139"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="IWO38_002781"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="IWO38_002781"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ0314795.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="IWO38_002782"
     CDS             complement(2262..2513)
                     /locus_tag="IWO38_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ0314796.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="IWO38_002783"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="IWO38_002783"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ0314797.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="IWO38_002784"
     CDS             3463..3681
                     /locus_tag="IWO38_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314798.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1027 a          797 c          688 g         1204 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caagaa
//
LOCUS       DACECY010000133         3622 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.140, whole genome shotgun sequence.
ACCESSION   DACECY010000133 DACECY010000000
VERSION     DACECY010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.140"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..87)
                     /locus_tag="IWO38_002785"
     CDS             complement(<1..87)
                     /locus_tag="IWO38_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ0314799.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYR"
     gene            162..440
                     /locus_tag="IWO38_002786"
                     /pseudo
     CDS             162..440
                     /locus_tag="IWO38_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
     gene            583..804
                     /locus_tag="IWO38_002787"
     CDS             583..804
                     /locus_tag="IWO38_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0314800.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            820..1662
                     /locus_tag="IWO38_002788"
     CDS             820..1662
                     /locus_tag="IWO38_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314801.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1725..3527
                     /locus_tag="IWO38_002789"
     CDS             1725..3527
                     /locus_tag="IWO38_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAQ0314802.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
BASE COUNT         1302 a          514 c          731 g         1075 t
ORIGIN      
        1 acgatagcta aggttataac gaagatagta gcccacggct acaataatca catcctgctg
       61 aaattgcttt cctttaaaat gattcatcgt cattcctcct gctatctttt tctattattc
      121 taccttattt gatagtagat ttaaaacttt gcaacagaac caaagaactc atcaagctgg
      181 ctaataaggg tcaagattat gaacatctag cagatgagat tgatgagctg agagacaagc
      241 gacagctcct tttagtagaa gatgcctccc tcagtggcga gaacgagcga atcaatgagc
      301 tgattgaatt tatccgcaag aacaaattcc gtaccttaga gtacgatgat aagcttgtaa
      361 ggaagataat ccagagcgtt acagtctatg aagaacactt cgtcatatcc ttcaaatctg
      421 gcatcgaaat ggaaatatga aaaccagaag taaatagcct atgattctac agtagagtcg
      481 tgggttttct tttgcttgct gattataata atattttaat aactattgtt attataaacc
      541 aaatgattta taatattctt agcgtaaact tggaggtgct gtatgactac ggcagaaatg
      601 attaaagaac tgtgtgagca aatgaatata agtgtttccg aacttgctag acgtattgga
      661 cagactccac agaatttcaa taagaaatta caacgagaaa cggtaacctt agatgagttg
      721 aaagcgattg ctgatgtgct aggtgtcaag tttgtgcagg cttttatatt gcctgatggc
      781 aatgaaataa agataggtaa ttaggaggtg taatttgtat tgaaaatcat aacaataaat
      841 gatgttgagt atgctgtatt tgctgctaat gaaggtactt ccaaaccaca acctcatatc
      901 atagaaacga aatctggtac tattccagaa ggaaaacaac tgagtttatt aaaagaatat
      961 cttaagcaga atgatatttc cccaataaaa ggagcaacaa catattggtg cattgacaag
     1021 gttcttaagc ttgattcaag taaagaaaaa actataagtg aaacaatcca taaacaaaaa
     1081 tacttatctt taactgaaga aaatattgag aaacagcata agtttgtagg tgcaagctct
     1141 aattatggga aagaaggtct aataatacat gatgttttga atgcatttcc tcttcataat
     1201 gatttaaata ctattgcgat gaagattgcg gtaattgatg taacaaattc aacacatctt
     1261 tctcaatata agagcaggtt gtctctatat gatttggcta aagtaattct ggagattcca
     1321 aattttgatg atcggttggc aaaaggagat cctcagttga taaatattat cgcaaggaac
     1381 attggtgcag ttaatatgtt ctcatttgca tccaagtact gtacatatca taatgtggaa
     1441 gtgtgtggga gagatgatta ctccatcttt gatggtatcg ttaaaaatac tcttccacac
     1501 tatatccaag gactaactac gaataagatt gatacgtggc gaagaagttt tgattacgag
     1561 gcatttaacg aatgtgttgg aaaactactt gatgaaaata atatacatat tccttttaga
     1621 agaagaaagt tagatcactt cttgtggtat gcgaataggt agaatataag aaaaaggcat
     1681 cgtaaaagtg attctggata aaattaatgg gggaggaaat aatagtggga ttatttgatt
     1741 ttctaaagaa aaaacaagga aaggttagta aacacgttga ttatcaactg gcttctagtc
     1801 tatttcgtac tggtaaagtt tttgctgaaa aattggaagt ggacatatca aatgtagatg
     1861 agtttcgtaa aacttttatc gcttttgatg tggaaacaac tggcctaaat tcttcttcag
     1921 atagaattgt agagattggt gcggtgctat ttaaagaagg agttccaatt caatcattcg
     1981 gaactcttgt caatccaaaa gttagaattg cagcctcagc ttcggcaata aataatataa
     2041 ctaatgaaat gctaaactct gctccaaatg agcaggaagt ttacccccag ttggtagaat
     2101 ttttaggaga tgcactagat ggaaaaatca ctatgtgtgc acacaatgca agtttcgatt
     2161 ttggattttt aagtaacaca ttttctagac ttgggtatga tgctaaaatt aagtatgttg
     2221 atactctgag tctctctaga aaacatataa aaggcattgt aaatcataag caagagactg
     2281 tagcggaata tttcggatta ataaatgaca ctgcccatag agcggaatca gatgcagata
     2341 tatgcggaaa gattttttgg ggtgttttag atattattga ggagtcgttg gaagaacaaa
     2401 aaaagcaaat agaaaagatg actccagaca taaaagaact tgaagcttgt gcttttattc
     2461 aagatattat tgagaaaaaa ggatgcgaca ccagttggat caggtatcga aaaaacagta
     2521 ataattatgt cgatgtaact tgtctatata cattcttaaa gtttaagttt gctaagaagg
     2581 gaaattacat cattattaga aaagatgcta caaatggaat tgatttacct atagaacctt
     2641 gtactgtaag tgaaggcggt acaacaaatg tgcgagtcta ttttcgtagc ccctttgact
     2701 taacgcctct ttcaaattat attttcagtg cttatagcga ttgttataat tctatgaatg
     2761 aatatatgag tgttagtaat tacactaaaa aagaagcaga gcaaagcctt agtatgctga
     2821 aatctatttc aaaatcagcg atgaaagagt tattattcga tgcaaaaaat cgtggatatg
     2881 atgagactga agttaaccta aatattgaaa aagtgatatc tagagaagat gtaattatta
     2941 acgggttgca taatcgagtt tcattaaaca agatacgaaa tatagggaat tgggagaaag
     3001 gatttgatgc aggttatcca tattgggaac gtggagaaac tgctaggaaa aatggcaaag
     3061 tagatgaagc tattgcactt tttgataaag ctcgatataa cggctatgat gctcctgcgc
     3121 tatatgattc ttatgctaaa gcttatcgaa aaattaagga ttacgataat gaaattctca
     3181 ttcttgatga aggaattatg cgtaaaacac ggcacgatgt tggtacttta gaggctcgta
     3241 gggataaggc aattaagcta ttatttgcta aacaagaggc agagcggata gctaaggaaa
     3301 aatctgattt tctaaaagcg aacaaaaaag aggatgcgat tagtgagcct agcaagaagc
     3361 gtggaagaca gattattcaa atgactgatg atggaacaat aataaaagaa ttcgatacta
     3421 tagcttctgc tgtaaaagaa gttggcgtta actcaaaaag tattagggat gctgcaaatg
     3481 gagttcaaaa acatgctgga ggatactact ggaaatacaa agattagcag ataatctaag
     3541 ataacctagg atcttcttaa tagatacccg tggtgctagt taattttaat atcctaaaga
     3601 atatctcaaa gttccttcaa aa
//
LOCUS       DACECY010000134         3395 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.142, whole genome shotgun sequence.
ACCESSION   DACECY010000134 DACECY010000000
VERSION     DACECY010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.142"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            646..963
                     /locus_tag="IWO38_002790"
     CDS             646..963
                     /locus_tag="IWO38_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ0314803.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(1054..1419)
                     /locus_tag="IWO38_002791"
     CDS             complement(1054..1419)
                     /locus_tag="IWO38_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314804.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1627..2109
                     /locus_tag="IWO38_002792"
     CDS             1627..2109
                     /locus_tag="IWO38_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314805.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2306..3196
                     /gene="pstS"
                     /locus_tag="IWO38_002793"
     CDS             2306..3196
                     /gene="pstS"
                     /locus_tag="IWO38_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ0314806.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1172 a          519 c          619 g         1085 t
ORIGIN      
        1 tattgctttt gcaaaattta tttacctgtt ttctggatca acgatagtgt acattatgct
       61 atccttgttt ggattttagg acgtttaaag aggcactttt ttacataact taatccatag
      121 accttttaag catgctgtga gataataata aagaagagtt taaagcgcac cccaaaccac
      181 ttccccataa gtgtattacg ctttaaactc ttttatattt gaattcacta agaagcatac
      241 catatatttg aatttactaa gaagcatacc atatttttga aaaaaagtga gaaaaaaggc
      301 ttataattag gatttagagt aattaattag atgtttattt tataggactg atactataga
      361 ttacaaatag tatcgtattt cgcaatctta aaattatctt cttactgtga ataagataat
      421 aaaatttgta tttgtccttt ttaggtaact tattttaatc tatatacctt tctggatcaa
      481 cgaaagtata ctttatatag tcataacgcc gatggtaact atgcactgta acgataatgc
      541 tgctaaaata tctttttacg aaaaacatca caaggaaaaa cttaactatg gtattatcta
      601 aacataattt tttcggtatt taatatttaa ttaggaggta atattttgct tttttatagt
      661 aattttatct taatagtagc gatattatta cttctgaata tatggatttt tgataggagt
      721 agaaattcaa gtataggttt tagaactaaa cgaagtttgt cttctaaaaa aaattgggta
      781 tactcacaaa ctatttttta tggaggaatt gttttaatta gtttactttc ttcaacactt
      841 tattctttga atattataga tgtttccacc tcaaattcaa tatctattat tggaatcatt
      901 attgctgcaa ttattacaca actgttttta gtgtttggag agaagaaaag aagtaaaaaa
      961 tagaaaaact agtgatagtt ttggtgatat aagaaaaaca tatatgtata aaaagaagaa
     1021 agtagcttct ctttgtacat atatggtttg tttttatgga tgccgagctt ttttataaga
     1081 taaggctacc gcccataacg acccaaggat ttgtggaact aagtaaatac ctgcaagaag
     1141 caagatcagc tgggtcggtt ttaaaggatg atagaagtaa cgccattcag taaaattggc
     1201 gcaaaataaa taggaagtga cagtagatat aatattggca atgaataaag atataatacg
     1261 cctgttcctt tgaaaataat atccagcaaa aagagataaa aatatcaatg tgatgaaccc
     1321 gcttacggaa tgataacgaa aatcaagcag taaggcgaaa tgcacataag gaacaaaaga
     1381 taaaagcaac ggaaggtttt tccagcaagt ttttatcatc tttcatcacc acctaacaag
     1441 tgttattatt ttgttagttt tattgtaaat tgaatcctgc aataatcaat tactttttca
     1501 ttatttctag attaaatgaa taaactctca tgccattatc tctaaaaaaa aagtttttcc
     1561 ccctaatttt tttagataga aaattgtata gtatgatgag agaaaaagta gtttggggga
     1621 acgttaatga gaagaaaagc agtaatcgat cggctttatt tatttttttg gttttttcct
     1681 atcgttttgc taccttttac atgggtaaat gctgttgtac aaggtgcgga gatattaggc
     1741 ctgatccaag gaagcggatt tgtggtttct aggaattttc cagctttgat tgttttctat
     1801 ttagtgataa gtgtaggtct cttattacca aaatctctga tcaatcacta caattggcag
     1861 ctgctttact tttcttgctt attagtattt acatctatgt tttctttgct gcctcgcttg
     1921 attatcggcc ctaatggttt gagcgagagt ggattgaagg atttagctta ttcctccatg
     1981 tatttcttga cgatcaaacc atggttctat ttagtaattg cctcatttat tcttgcaaca
     2041 tttttttact tctggacaga aatcaggata aaaagtgaac gatctgtatc tataaaaaat
     2101 aactattgat ggtggatcat cagtaaaaaa acatatgagc tgcgtaaaat gcatgtttct
     2161 catatgcttt ttttattatt atgtaaagaa tgcgaaaaac gtttacatga tttttacata
     2221 acttaactat atttttacag caaccatacg agggatttac agtcgactga taaactttag
     2281 ttgtaaataa gaaaggagca ataaaatgaa aaaagtattg tttactctag gtatgatggg
     2341 aattctttta gccggatgcg gctcaggatc aggaaataca gatggctctg ccacacaaaa
     2401 taattcaagt gactcaaatg aacaagtaaa aatcgttgcg gtaggttcga cagcattaca
     2461 gccgttagtc gatgcagcac aagaaagctt tgtacaagaa aatccaaatt atcaaatctc
     2521 tgtacaagga ggaggaagcg gaactggttt gagccaagtc gaagctggag cagttactat
     2581 cggaaactct gatgtatttg cagaagaaag agacggagtt gatgcttcaa aattagtgga
     2641 tcataaagta gcagtggtcg ggatggcacc aatcgtcaat aaagatacag atgtcaaaga
     2701 tatcaccaaa caggaattga ttgatatctt tacaggaaaa atcactaact ggaaggaagt
     2761 tggcgggaaa gatcaaaaaa tcaacgtcgt gaaccgtgca aacggcagcg gtacccgcgc
     2821 gacattcgaa aaatggggac tggatggtgc tacccctgtg cagtcccaag aacaagattc
     2881 atcaggaaca gttcggcagc tggtaagtca aacaccagga gcaatcagct atttagcttt
     2941 ctcatatctc gatgattcca cacaagcact aagtatcgac ggtgtagaac caaaagaaga
     3001 gaatgtagca gataacagct ggggaatctg gtcatatgaa catatgtaca cgaacggaaa
     3061 accttcccct gaagtccaaa aattcttaga ctacatgatg acagaagaaa tccaagaagg
     3121 acctgtcaaa gaattgggct atttaccaat cacaatgatg gaagtagaac gtgatcacga
     3181 aggaaatatc aaataactag cagataaaca tgccacggta gtcgttacta ttaaggaaaa
     3241 atcaactatg tttccccaca tatacatttt tcctttaatt gtgaaaaata gttcctcata
     3301 ttttgccgtt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tattcgaaaa
     3361 aaagagctac gacatagaac tgtcgtaact ttttt
//
LOCUS       DACECY010000135         3380 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.143, whole genome shotgun sequence.
ACCESSION   DACECY010000135 DACECY010000000
VERSION     DACECY010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.143"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            89..1168
                     /locus_tag="IWO38_002794"
     CDS             89..1168
                     /locus_tag="IWO38_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0314807.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            complement(1497..1739)
                     /locus_tag="IWO38_002795"
     CDS             complement(1497..1739)
                     /locus_tag="IWO38_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0314808.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(1771..2328)
                     /locus_tag="IWO38_002796"
     CDS             complement(1771..2328)
                     /locus_tag="IWO38_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0314809.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(2341..2529)
                     /locus_tag="IWO38_002797"
     CDS             complement(2341..2529)
                     /locus_tag="IWO38_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0314810.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(2542..3108)
                     /gene="amaP"
                     /locus_tag="IWO38_002798"
     CDS             complement(2542..3108)
                     /gene="amaP"
                     /locus_tag="IWO38_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0314811.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT          930 a          768 c          680 g         1002 t
ORIGIN      
        1 cttagccatt tggaaataaa aaaacgccac gtgaattcca tgttatattg aagttaccac
       61 aactctcaat agacaaggat gaattcacat gacgcaaaac catgataaca cagtttcacg
      121 caagggaaaa cacctaacct actcagaacg ttgccaaatc gccatcctga aaaaggaaaa
      181 ctattccaac cgtcaggttg cggatgtcct gggctgcgtg ccgcagacca taaacaacga
      241 aatcaaacgc gggaccgtta ctcagcttaa ccgccagaat cagaacggga aggtctacga
      301 ctattatcat gacgtctacg acgccgatgc cgggcaggcg gcctacgaga agaaccgctt
      361 gaactgcggc agacggccct tatgggccga cacggacgcc ttcattgaat gggcggacga
      421 catgatgctg ttggaaaaat ggtccccgga cgtggtggtg ggctttgcca gaaaacacga
      481 attgttcgac ccggccatta tccctgggac caccaccctc tatcagtgga tcgaccgtgg
      541 catcatgaaa acgaagaata tggacctgct agaaaaactg tcccgcaaac cgaaatcgtc
      601 ccgtcatggc aaccgcccga ataaacgggt gctagggaaa tccattgaag agcgcccgga
      661 ggcggttggt acccggaaaa ccttcggcca ttgggaagtc gacacggtcg taggaaacaa
      721 gaccaagtcg gatgccgtgc tgctgacgtt ggtggaacgg cagacacgct tcgaggtcat
      781 tctgaagctg gccgggaagg atgccgaatc ggtcaaccaa gcggtccgac agctgagaga
      841 acgggctggt gcgtacttcg gccagctgtt caagacggtt acctcggaca acggctcgga
      901 gttcgcggga ctgcacgatg ccttgcagga tgtggcaaac atctacttca gccaccccta
      961 tgcttcctgg gagcgcggga cgagcgaaaa ccaacacaag ctgattcgcc gtttcatccc
     1021 caagggcagc ccaatcggca acgtgagcga aaaccaaatc ttgcggattc agcgttggat
     1081 gaacgattac ccgcgaaaga ttttgggcta tgcaacgccg catgacacgt tcgtccaggc
     1141 atttaggcag gaacggttga cggcttgaca cgtgggccct tgacaacgag cctccctcgg
     1201 catggcttgt gtctggaaag ctggccgggg ccagccagtc gggagactac catgcctcag
     1261 ctcattgtca agggcccact cgattagaaa aattagacgg ttggtggtaa cgagcaaccg
     1321 ggtcaccatg ggtggctaac ttaaacttga aatttacgtt tttttatttt taaagatatt
     1381 ttttcataag gaataaagaa tacttcatat aaaatatata aaaaagaacc gcagcgagtg
     1441 tcgctacggt tctcaaattt gttcttcatt tcaacataag tttttcttca atttgcttat
     1501 aatttacgtg caaagaatga aacgactaat actaaaatga ttgcaccgat caatgatgga
     1561 atgatagcca ttcctgcaat tgttggtccc caagttccta gtaaagcttg tccaattgct
     1621 gaacctacta aaccagcaat gatgtttgcg atgatcccca ttgatgatcc tttattcgtg
     1681 attgctcctg caattgctcc aatgataccg ccgacgatta atgcccaaat aaatcccatt
     1741 ttaaattcct cctaactgac ttcttacttc ttattgtaca cgtggttcgt tattttcttt
     1801 gactttttgt gcacctttgc ctacagcaga ttttgcttta tctgtttggc gtgaagcaaa
     1861 ttgtcctgtg gattttgctg cttctgttac tttgtcttgt actgtttctt gatctttttg
     1921 gtattcttct tgtgttttga tgtctgcaac attcacattc acttcgataa catctaaatg
     1981 tgtcatattg tttacttcag tactgatcaa ttctttgatt tggttgtaaa tatcttcgat
     2041 gtctttaccg tattctacga tcacatctag atccacagct acttgtttct tacctacttc
     2101 tgtattgata cctgctgtta cgttatctgt gttgactaat ttttcagcca tatttgagaa
     2161 aaatccgcca tctaatgtca atagaccatc tacatctttc aatgcataac cgatgatttt
     2221 ttggatgact ttgtcttcat aagtcaattc accattcatc atgcggtttt ctcctttttc
     2281 atttgccaaa actgatccat ttgttgtatt catgtttttg ttttccatct ttgtttcctc
     2341 ctatgattta aaaaattgat caactattcc tgactgtttc aaataaaaac cgattcctgc
     2401 gccaattgct gtcatgatta tgatcaacaa tgtcttgaaa aatccaatcg ttaaccataa
     2461 aacagctagc agtagtccaa gacccccaaa gataataggg agcttgtatg ctgaaaatag
     2521 ttctttcatt atttggccct cctattccac tcgagagtgc ttgtagtctc ttgtatcttc
     2581 ttgttcttca taaccgctgt atgtgactgc cactttgacc gtttcttttg ttccgagtac
     2641 tttagttact tcttcttcga tatcttgcat caatacacct gttttcccta ctaatgaaga
     2701 agttcgtttc aagtctccgt tgacatggat atggattcga tttttcgttg aacgtatccg
     2761 tactttaggt gaatgaatga actcttcttc gtgtaaatgg gaacgtacca acccttcgat
     2821 cgcttttttg tcgattgtta gtttgccatc ttcccgtttc ataacgaaag ttcctcttga
     2881 ctttggatag aaaagaacga ctagcaagat gataatagca agaacggcaa atactacagc
     2941 tgcccagaaa atatattgtt gcatgtagta attcgttaaa gtaaagaagc gaaatcgttc
     3001 caattgttca ggcataatat acggcgcatt gatcaacgca atcgctagaa atactgaaag
     3061 tagcagtaaa ctcatgataa caccgattat ttttaacccc ttattcattt tccacctcct
     3121 ataaacttat ttttaccctg taaataactc taccgacaat cacagtatag atagcgaaca
     3181 gttagcatag catcagtaaa taaagcgcta ccaaacattg ctggatcata acggcgtttt
     3241 attgccagtc tgatactgaa ataacaattg cttacttatt ctctatttac ttttgattgc
     3301 tggctatact taaaggatat tattcaagat taaaaattgc aaagattatg ctcagttatt
     3361 attacaagat tctatttatt
//
LOCUS       DACECY010000136         3374 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.144, whole genome shotgun sequence.
ACCESSION   DACECY010000136 DACECY010000000
VERSION     DACECY010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.144"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(61..831)
                     /locus_tag="IWO38_002799"
     CDS             complement(61..831)
                     /locus_tag="IWO38_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HAQ0314812.1"
                     /translation="MNPTLITKKELLKKLDISTGVLADLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCFKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDIYNQEENQTRHIRNLSDAKLE
                     EEARKVSNYNKKMAAKYVNEEEFCFSKTKIYKRTRGAS"
     gene            complement(1473..2144)
                     /locus_tag="IWO38_002800"
     CDS             complement(1473..2144)
                     /locus_tag="IWO38_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0314813.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLAENYDNHVVIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGY
                     SFILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEM
                     FINAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPNTIYFKRV
                     GGGIT"
     gene            2667..3257
                     /locus_tag="IWO38_002801"
     CDS             2667..3257
                     /locus_tag="IWO38_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ0314814.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
BASE COUNT         1075 a          575 c          472 g         1252 t
ORIGIN      
        1 attaagtcga gcacaacgac gttttgaagg aactttgaga tattctttag gatattaaaa
       61 ttaactagca ccacgggtac gtttatatat ttttgttttt gagaagcaaa actcctcttc
      121 gttaacatat tttgcagcca tttttttatt ataattacta acttttcttg cttcttcctc
      181 taatttagca tcagataaat tacgaatatg tctagtttga ttttcttcct ggttatatat
      241 atctttatct tcaatcaatt gctcttgatt tattaaagct aatggaaatt tatatacttt
      301 tctgctgttg cccgaactat ctttttcatt aacagtatat atttgtcctg ctaaatatac
      361 ttctcccaga tacgtgtgtt cttttggttt aaatgtttca aataaatgaa tctttacaaa
      421 attacttttt gaattggcta aaactttatt ttgattctta tccaatactt gatccccttt
      481 tagccccata ccggtatatt gtaaaatacc atttaaccac ttatctttat aaacattact
      541 tcctgtttga tcactaaaca gtacaagagt attagtttga tgagatcttc tcattccacc
      601 ctgtttgaaa catttaaact tctctgaaat ttctttgtta gtatatactc taccaacttc
      661 taactcacct aatatgtttt tagaccaatt ttctcgccat gtaataatct tatcaagatt
      721 aaacatttct ccctcttttg gcatgccatt tctaattaaa tcagctaata caccagtaga
      781 aatatctaat ttttttagta attctttctt agtaatcaat gttggattca taacaatgca
      841 gctcctaatg atatcaatat aattgcatta gataacttga aaaatcgaaa aaaactcatt
      901 ttgtgtcaag ttatctgtaa tatataaaaa catcattatt atacctcatc tatgtgaatt
      961 tttactattc ttaccttagc atattcttta taggtatatc agagctaatc aaactaaaaa
     1021 ataccccaag tcttcttatg taaaaacacc ggttattact taaatatgac ttaggtaaaa
     1081 caatgagtga tccaattata aaattttatg gcgaataatt tagtaagcgc tacatattga
     1141 atgatttaga aattttgaaa aagtagctag ttccttttat accagaaaag taaacctaat
     1201 aaaaaacaca actaattttt agctgtgttt tttaagttac aataattatc tatttaatag
     1261 tcttaattca ttaaaacaaa aaactcaact ttacaaggat atggtttaaa aaatttattt
     1321 atccaaccca actagttttc gctgcttacg atttgcttca tctgctacgt acttcattac
     1381 tgttttaaat tctttcgctg aggcgttttc tgataaatca gcgatacttt tattatactt
     1441 cttcacgatt tgttgtactt gttctttctc agtcatgtaa ttcctcctcc aactcttttg
     1501 aagtatattg tattgggtag tactaattga atattatctg ctgcataatg cacttcagaa
     1561 atgtcgtttt gataatcctt aatcattatg tgaaggacta cttcaggatg tctatttttg
     1621 actttttcaa tattctctct ggacttaaaa aacgcattga taaacatttc ttttggcaca
     1681 tgacgtcctt cagctagttc acgttttcga gtgaaatccc aggcaacttt gggatcttgg
     1741 taaacataga aaatcgtaac gcgaaagtta tttttaaggg ctcgaataat atttttctct
     1801 gcactaagga tagcaaaagt atcctctagt atgaaagaat agccatgttc cagtacatat
     1861 tttaatgatt gttcaactaa ccaagaagaa gctttctgaa aaacacttga gttctttcca
     1921 ttgtattctg gaaattgtgc tctaaaatag tccgcatcaa tcacaacatg gttgtcataa
     1981 ttttcagcta aacctagtgc aacctctgtt ttacctgctc ctgggcttcc agccatgaat
     2041 accgcatctt tttcatcatc agggattttt cccttgatga ttgtttcaat aaatttatct
     2101 ttattgaccc tagcgtaatc taaatacttg tctttaggtt ccaagattgc accttctttc
     2161 cttcttctat attcatacta attatacgat atttatataa aaaatataac ggtcatttta
     2221 accatattaa catttagtca cagtaatagc agatatgctc gcaaaatgag atatgaatgt
     2281 acgcagaatc cttgttttct acggcattcg ctgttttttc attggactag ttatatattt
     2341 ctagggttgt tttcatgtct gagtgcccga tatgatttat ttttcgtttc tataattact
     2401 ttttgccttc cagtttcttt taagattgta ctcaagcaag tgggaattgg ttcttttgtc
     2461 atccatgtat gcaaaatgtt ataatgctgg taaacaaaaa tgattacctg tcatttttat
     2521 ttagttcgta tttaattaag ttgactgtct gtatactctg tctaaaactt aagaattgtt
     2581 gttttcgctg tcgctatcca aatttaatga atgtctgtat gttaaaactg ttgtccttgt
     2641 catagatagt aaaggagagc ttgaaaatga aaactgcgat tacgtttcta ttagaggaat
     2701 atgccgattg ggaaggtgct tatattacta gcacactaaa ccaaaaagag ggatggcaaa
     2761 acaaaactgc ttctatccaa tcagaggtca cttccattgg aggattaaaa acaaaaatcg
     2821 attacttaat cgaacaaata cctgaaaaag ttgacctttt tattatgatc ggtggcaatt
     2881 catgggacat aaaaaatcat catttatatc aaataatcga taaattgtta aaaacagacg
     2941 taccagtagc tgctatttgt ggggcagttg attatttagc tcagagcgga ttattgcaag
     3001 gttacaaaca caccggaaat gcgcaatttc cttggttaga atcggaaaat tatcagaata
     3061 aagaagattt tatagaacaa caagctgtga aagatcgtaa tttgattact gcgaatggaa
     3121 cagctcctat agaattcaca gacttgattc ttcaagcagt ccattttgaa acagaagaaa
     3181 taagagaaaa aaagattcgt ctatacaaac ttggattcta tgattactat aaaaagtacg
     3241 gagatccttt ctcctaatca ttctcttcaa ttaaatactc cttatcaata gtatgacaag
     3301 taattttact gatggttatt tgtcacactt tttctgacat ttatcaaaga agatacactc
     3361 tatttatatt cata
//
LOCUS       DACECY010000137         3281 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.145, whole genome shotgun sequence.
ACCESSION   DACECY010000137 DACECY010000000
VERSION     DACECY010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.145"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..584
                     /locus_tag="IWO38_002802"
     CDS             <1..584
                     /locus_tag="IWO38_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0314815.1"
                     /translation="AFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIELS
                     KKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMAN
                     SGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHVL
                     EGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(661..1836)
                     /locus_tag="IWO38_002803"
     CDS             complement(661..1836)
                     /locus_tag="IWO38_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0314816.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            1918..2298
                     /locus_tag="IWO38_002804"
     CDS             1918..2298
                     /locus_tag="IWO38_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ0314817.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            2372..2734
                     /locus_tag="IWO38_002805"
     CDS             2372..2734
                     /locus_tag="IWO38_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314818.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTVL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         1113 a          539 c          621 g         1008 t
ORIGIN      
        1 tggcattccc acaactagat ttaaaaagtg aaaaaggacc aaaagctgtt atcaaaacaa
       61 accgtggtga cattactgtt caattattcc ctgaactagc accgaagacg gttcaaaatt
      121 tcattgaact ttccaaaaaa ggttattatg atggcgtgat ttttcaccgc gttattcctg
      181 atttcatgat ccaaggcggc gatccaactg gaacaggaat gggtggcgaa agtatttatg
      241 gcgaaagttt tgaagatgaa ttcagtcgtg aactgttcaa ccttcgcgga gctttatcta
      301 tggcaaattc aggtccaaac acaaacggaa gccaattctt tattgtaaat aacacaaatg
      361 tgccagctaa tatgcttgga caactagaag gtgctggatt cccatcagaa atcattgaag
      421 cgtataaagg tggcggaact ccttggctag atttccgaca tacagtcttt ggacatgtac
      481 tagaagggat ggacacagta gatgaaatcg caaatgtaca acgtggtcca caagaccgac
      541 ctgtacatga tgtagtgatt gaaacaattg aaatttcaga atagtttggt atggtacgga
      601 gtaattatga aaaaggacga aacctagtaa ggtctcgtcc ttttattttg aataaatgat
      661 ttaatcgatt gtctctaaag catacttgat tcgattaagt ccttccagta aagtttcttc
      721 tggacaagca atattcaagc gcatatgccc atgaccggaa ggaccaaacg tgatgccagg
      781 atttaatacg acttttcctt tccgaacaag agtgttctcc aattcttggt cgtctgaaat
      841 ataggcggta aaatctaacc acattaagta agttccttca gggttcatta ttctcacttt
      901 cggaagatac tgttcaaaaa actgattaac tgtttgaaca tttttttcta gataaggaat
      961 cagttggctt aaccattctc cacccgtctc ataagccgct tgggttccaa tcagtccaaa
     1021 agtgttgatt tcgtgctgtt ggttcatcac taaatgtttt tcaaaatcag ctttcatctc
     1081 tggatttttg ataaatacca ttgaattttt gattgcagct agattgaaag tcttggttgc
     1141 ggaagtaaaa gcaactagta gatcatctaa attaggatca atcgttagca tggaagtaaa
     1201 tgtatgatcg aataaagtta aatcttgatg gatctcgtca ctaaacagaa tcacttgatg
     1261 ttttaaacac aattcgctta actgtttcaa ttcttctttg ctccaaacac gccctccagg
     1321 attgtgagga ttgcataaaa tcattgcttt tacattgttt tcttcgattt ttttctccat
     1381 gtcagacaag tccatgatga aatgattatt tttttctagc aaagagcttc gaacgatgtg
     1441 acgttgattt gtttctataa tagaggaaaa tggaggataa actgggtcat gaatcaaaat
     1501 agaatcgcct ggttttgtaa aagtttgtac tgctgcagct aaactggtta acacaccact
     1561 tgtaaaaacg atattttctt tcgtcaactc cacagcatgg tgttggcgtt cccaatgaat
     1621 gatagcctga tatagtttat cagacagagt ggcataacca aagattccat gtttgatgta
     1681 ttcactaaac gctttagcta ccccaccggg agacaagaag tccatatctg ctacccacaa
     1741 aggaagtaag tcatccattt gatatgtctc agcaattgcg tcccatttta cactttctgt
     1801 atcttttctt gaaatctgtt tatcaaattc gatcatctta agacctccag tcattttcgt
     1861 cgcttctttt tttgagctat ttacagtata atgataaatg cgaagagagg gaagtagatg
     1921 acttataaaa ttggacaaat aattgaagga aaagtgacag ggatacagcc ttatggcgca
     1981 ttcgtttctc ttgataacga gacgcaagga cttattcatg tttcagaagt acaatccggt
     2041 tatacgaaaa atatccattc attgttaaaa gtcggacagc cagtaacagt tcagatcatc
     2101 gacattgatg aatattccaa aaaaatcagt ttatctttaa gaacgttaga aaaaacaaat
     2161 ccagctgttc catatcgcag aaaaaggtat ttcacgaata aaaatcgtaa gatcggattt
     2221 accactattg cggagcagtt acctatttgg atagaagaag cattatctga actagaacgg
     2281 aaagaaaata agaaataaaa agtgtatttt ttctgaaaat gtgctagaat ttttatgtaa
     2341 gccatagaac aagttgatcg aggtggaaat tttggcaagc aaaagagttg ctggtacaat
     2401 tatgttgcat ttggaggatg gtactaaaaa atttttggtc cattcaattg atgacaagtt
     2461 ggaatttgcg ttagcggaac tttctgaagg aaagactgtt ttagcgaata tacttaattt
     2521 cttaaaagaa atagttcata tcgatgttag caagattagt ttgatggaat tgacaaatac
     2581 acatattaaa gaggaaaatg ttccgttgtt tgtctttgaa actgaacaaa agaatcaaag
     2641 agaagaatta ggcgaaggat atatttggga agaaccaggt caaatgcgct ctgttcttgg
     2701 aacttacgaa gtagagggag tgcctttctt ctaaaataca gattaagcga acaaagaagt
     2761 ttgaattttt ttcaaacttc tttttttatt gtttggtcca aaaaatacaa tgaaatgttc
     2821 tgctaaagat agttttttag tgcattttat tttcaaaata tgttgaaaac ggttgtataa
     2881 cgaataataa taattgatcg aaaaatcatt tcacaaagta atgcttaatt tatagttgac
     2941 caaggaagaa atacatgata aatttaacag cgttgattct gaagtggatg ttatatcaca
     3001 tttctaaaaa atttaaaaaa tagttattga catgttacta acgacatgat aagataactg
     3061 agtcgttaaa aaagaaccac ttgcgaagct tgattctatc gaaaaaaact tcaaaaaagt
     3121 tcttgacaaa taacaaacga taagttatga taaatgagtt gctgaaagat ttcgaaaaac
     3181 gaaatcgaaa aaaactttta aaaaagagtt gacaatcgct tgtctctctg atatgatata
     3241 aaagttgcta cggcagccaa aaacgaatcg ggaaacactc c
//
LOCUS       DACECY010000138         3229 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.146, whole genome shotgun sequence.
ACCESSION   DACECY010000138 DACECY010000000
VERSION     DACECY010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.146"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..183
                     /locus_tag="IWO38_002806"
     CDS             <1..183
                     /locus_tag="IWO38_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314819.1"
                     /translation="IVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNEL
                     FRDTLNDPNPLFDNLN"
     gene            207..542
                     /locus_tag="IWO38_002807"
     CDS             207..542
                     /locus_tag="IWO38_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314820.1"
                     /translation="MPAFYEVKMMYRNPKNIKQEIKLFFFYLVDLGIVAGSVMASVYF
                     AKLMPISPMMKFALYLLAFVVGVWMCVRTPNHPKERNILMLYYLFKMDRNRYHEVEFK
                     RNSRGKIEL"
     gene            539..1156
                     /locus_tag="IWO38_002808"
     CDS             539..1156
                     /locus_tag="IWO38_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314821.1"
                     /translation="MIVAESNENKLSGVFDKFLELVIPKDKDMCELLPYKKILKDGTL
                     VTNDNKYQAFFKVKTTDLTSISEDGFMRMVNKLTYLNRVYLEPYKIYSLTYPTETVNQ
                     AIYWKHRIQEAKRSLLSNNDPNLETYLKWNLKLAYDNYERVDLVSTLPELMFVICVYG
                     KTQKEVKNHADVMKQAGGRELDLQRITEGDLINVITKLMNPNTSL"
     gene            1173..1640
                     /locus_tag="IWO38_002809"
     CDS             1173..1640
                     /locus_tag="IWO38_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314822.1"
                     /translation="MLKHCRKGFCKVIKGKNAADLIFEWKESNLINVRGKNGSGKTER
                     VMASIDNSRPRSRALVITDEKNHYKGIRTIEKKYLTNNDLKVVDDILNIYPDFNLVVF
                     DETIPVTEIKGFYQSFSNLANKYEEVLFVWITEEDSNSKSAFFSEKNAFLHSI"
     gene            1656..>3229
                     /locus_tag="IWO38_002810"
     CDS             1656..>3229
                     /locus_tag="IWO38_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tra protein"
                     /protein_id="HAQ0314823.1"
                     /translation="MIVSKKLARNLVSEGYDLRLVEKYQPSGGIKFDERYISTGDGYV
                     TSIAVIEFAEDVNSRWLTDIMAIPNTIATFDVCTADKEDLLHKINTSIGELDYRSTNE
                     NRTTDKTDALLDKYNLEDFAQEITKRGEVVKTVHARIFVYSDSLEELEKRISEIRKDL
                     ASVNHKAIVPQFRMKDEWMSLFQDGMEQESKLFAKKGLTVPAKNIGGGIPFHHKSLKD
                     PRGFYLGTTSTGGAFIFDPFYSTKQRRSFNGFILGKMGFGKSTLIKQLQEGLQAKNCF
                     IRGFDKSGEFEGMVKKAGGVYVDLGGGDMVVNPLQIYATVMKKDQFGNDVVDESKSFQ
                     NHITKCSNQIRFLEPEIDTATVREYTLMLQDFYIHYGILPKEWREARDDNERNQLNTN
                     ICNLPNDSYPIYGEFLKFIENQQFKIKNATPERVRSIELIKLIVTDMCTTYPQIFNGV
                     TTMPNFDTTKIVYYNIDSISKMSAPVFHCQLFTALTTIWNSALQNGKIQKENFKKYGS
                     DYYKYAQDFMVLIDEC"
BASE COUNT         1207 a          410 c          623 g          989 t
ORIGIN      
        1 atcgttgaag acagaaagtc agcgaaaaaa tttgattcaa atcatgaaac agtgattatt
       61 gatagtgaaa tccgtgaaaa tgataaggga tcaaaaccaa gacgaagatt cacttatgaa
      121 gacaacgaat tattcagaga tacacttaat gatcctaatc cacttttcga taatttaaat
      181 taaattaaaa gccggcatga gtgattatgc cggcttttta tgaggtgaaa atgatgtata
      241 gaaatcccaa aaatataaaa caagagataa agctattttt cttctattta gtcgatttag
      301 gaatagtagc cggaagcgtg atggcaagtg tttactttgc aaaattaatg ccaatttcac
      361 caatgatgaa atttgcactg tacttattag catttgtagt gggtgtttgg atgtgcgtta
      421 gaacgcctaa tcaccctaaa gagcgaaata ttttaatgct gtattatctc tttaaaatgg
      481 atagaaacag ataccacgaa gtagagttta aaagaaatag tagggggaaa attgaattat
      541 gattgttgct gaaagtaatg aaaataaatt aagtggtgtt tttgacaaat tcttagaact
      601 agttattcca aaagataaag atatgtgtga acttttacca tataaaaaaa tattgaaaga
      661 cggaacgtta gtgactaatg acaataagta tcaagccttt tttaaagtta aaacaactga
      721 cttaacttct atttcagaag atggatttat gcgtatggta aacaaactca cttatttaaa
      781 tcgagtatat ttagaaccat ataaaattta ttcattaact tatcctactg aaacagtaaa
      841 tcaagcgatt tattggaagc atagaataca ggaggcaaag agaagtttat tgagtaataa
      901 tgatcccaat ttagaaacgt atctgaagtg gaatctaaag ctagcttacg ataattatga
      961 aagagttgat ctagtatcga ctttaccaga gttgatgttc gttatttgtg tttatggaaa
     1021 aacacaaaaa gaagtgaaaa atcatgccga tgtaatgaaa caggccggtg gtagagaatt
     1081 agatttacaa agaattactg aaggggattt aatcaatgtg ataactaaat tgatgaatcc
     1141 caatacttct ttataaggcg gtgcttatta ttatgttaaa gcactgtaga aaaggatttt
     1201 gtaaagtaat taaaggtaaa aatgctgctg atttaatctt tgaatggaaa gaatctaatt
     1261 taattaatgt acgtgggaaa aatggaagcg gaaagacaga aagagtgatg gctagtattg
     1321 ataattcaag gcctagatca agagcattag taataactga tgaaaaaaat cactataaag
     1381 gcatacgaac gatagaaaaa aaatatttaa ctaacaatga tttaaaagta gtagatgata
     1441 ttttaaatat ttatcctgat tttaatttag ttgtttttga tgaaacaatt cctgtgacag
     1501 aaataaaagg attctatcaa agcttttcca atctagcaaa taaatatgag gaagttctgt
     1561 ttgtttggat tacagaagaa gatagtaata gcaagtcagc gttctttagt gaaaagaacg
     1621 cttttttaca ttctatttaa aaaggagaat tgaacatgat tgtaagtaaa aaactagcaa
     1681 gaaatctagt aagtgaaggg tatgacttaa gattagtaga aaagtatcaa ccgagcggtg
     1741 gaattaaatt tgatgagcgt tatatttcta ctggtgatgg atatgtaact tcgattgctg
     1801 ttattgagtt tgcagaagat gtaaatagtc gatggttaac ggatataatg gcaattccaa
     1861 atactattgc aactttcgat gtatgtacgg cggataaaga agacttatta cacaaaatca
     1921 atacgtctat tggagaatta gattaccgat caacaaatga gaatagaaca actgacaaaa
     1981 cagacgcttt actagataaa tacaatttag aagattttgc acaagaaatt acgaaacgtg
     2041 gtgaagttgt caaaactgtt catgctcgta tttttgttta tagcgattct ttagaagaat
     2101 tagagaaaag aattagtgag attcgtaaag atttggctag tgtcaatcac aaggcaattg
     2161 ttccgcaatt tagaatgaag gatgaatgga tgtctttatt tcaagatggc atggaacaag
     2221 aatcaaaatt atttgccaag aaaggcttaa ctgttccggc aaaaaatatt ggtggcggta
     2281 ttccatttca ccacaagagt ttgaaagatc cacgtgggtt ttatttagga actacgtcaa
     2341 ctggtggtgc ttttatattt gatccatttt attctacgaa gcaaagacgg tcgtttaacg
     2401 gctttatttt aggaaagatg ggttttggta agtcaacttt aattaaacag ctacaggaag
     2461 gcctacaagc gaaaaattgt tttattcgag gatttgacaa gtcaggtgaa tttgaaggca
     2521 tggtgaagaa agcaggcggt gtatacgtcg atttaggcgg tggtgatatg gtagtaaatc
     2581 cattgcaaat ttatgcaaca gtcatgaaaa aagatcaatt tggaaatgat gttgtagatg
     2641 aatcaaagag ttttcaaaat catatcacta agtgtagtaa tcaaattcgc tttttagaac
     2701 ctgaaattga tacggcaact gtacgtgaat atactttgat gttacaagac ttttatattc
     2761 attatggaat tttgccaaaa gaatggagag aagcgagaga cgacaacgaa cgaaatcaat
     2821 taaatacaaa tatttgtaac ttgcctaatg attcatatcc aatctatgga gagtttctta
     2881 aatttattga aaatcaacaa ttcaaaatta aaaatgctac tcctgaacgt gtccgttcta
     2941 ttgagctaat taagcttatc gttacggata tgtgtacaac ttatccgcaa attttcaacg
     3001 gagtaacaac aatgccaaac tttgatacaa caaaaattgt ttactataac attgattcta
     3061 ttagtaaaat gagtgcccca gtattccatt gccaattatt tacagcgtta acgactattt
     3121 ggaacagtgc cttgcaaaac ggaaagattc aaaaagaaaa ctttaaaaaa tatggcagtg
     3181 attattataa gtatgcacaa gatttcatgg ttttgatcga tgagtgtca
//
LOCUS       DACECY010000139         2944 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.147, whole genome shotgun sequence.
ACCESSION   DACECY010000139 DACECY010000000
VERSION     DACECY010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.147"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            6..977
                     /locus_tag="IWO38_002811"
     CDS             6..977
                     /locus_tag="IWO38_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAQ0314824.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            970..1998
                     /gene="vanB"
                     /locus_tag="IWO38_002812"
     CDS             970..1998
                     /gene="vanB"
                     /locus_tag="IWO38_002812"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAQ0314825.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVHADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            2004..2612
                     /locus_tag="IWO38_002813"
     CDS             2004..2612
                     /locus_tag="IWO38_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAQ0314826.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT          780 a          624 c          812 g          728 t
ORIGIN      
        1 aaccaatgag aaaaagtgtg ggcattactg tttttggatg cgaacaggat gaggcaaatg
       61 ctttccgcgc tttatcgccg gattttcata ttatccctac gctgattagc gatgcgatat
      121 cggcagacaa cgcaaaattg gccgctggca atcaatgcgt tagcgtaggc cataagtccg
      181 aggtttccga ggcgacaatt cttgcgctga gaaaggtcgg ggtaaaatac atttctaccc
      241 gcagcatcgg ctgcgatcac attgatacga ctgccgccga gagaatggga atctcggttg
      301 gcacggttgc gtattcgccg gacagcgttg cggattatgc tttgatgctg atgctgatgg
      361 ccatacgggg tgcaaaaccc accatgcacg ccgtggcgca acaagatttc agattggatc
      421 gtatccgggg gaaagaactg ggggatatga ctgtgggagt tattggaacc ggccatatcg
      481 ggcaagcggt cgtcaaaagg ctgcggggat ttggatgcca tgtgctggcc tatgataaca
      541 gccgaaaaat ggatgcagat tatgtccagc ttgatgagct tctaaaaaac agcgatattg
      601 ttacgctcca tgtgccgctt tgtgcggata cccgccatct gatcggtcag aagcaaattg
      661 gagagatgaa gcaaggcgca tttttaatca acaccgcgcg cggagcgctt gtcgataccg
      721 gggcgctggt ggaggcgttg gaaagcggaa agctgggtgg tgcggcactg gatgtgttgg
      781 agggcgagga tcagtttgtt tataccgatt gctcgcagaa agtgcttgac catccctttt
      841 tgtcgcagct tttaaggatg ccgaatgtga tcatcacacc ccatacggcg tactacactg
      901 agcgtgtgct gcaggatact acagaaaaaa caatcaggaa ttgtctcaat tttgaaagga
      961 gtttacagca tgaatagaat aaaagtcgca atcatcttcg gcggttgctc ggaggaacat
     1021 gatgtgtcgg taaaatccgc aatagaaatt gctgcgaaca ttgatacgga aaaattcgat
     1081 ccgcactaca tcggaattac aaaaaacggt gtatggaagc tatgcaagaa gccatgtacg
     1141 gaatgggaag ccgacagtct ccccgccata ctctccccgg ataggaaaac gcatgggctg
     1201 cttgtcatga aagaaagcga atacgaaaca cggcgtattg atgtggcttt cccggttttg
     1261 catggcaaat gcggggagga tggtgcgata caggggctgt ttgtattgtc tggtatcccc
     1321 tatgtgggct gtgatattca aagctccgca gcttgcatgg acaaatcact ggcctacatt
     1381 cttacaaaaa atgcgggcat cgccgttccc gaatttcaaa ttattgataa aggtgacaag
     1441 ccggaggcgg gtgcgcttac ctaccctgtc tttgtgaagc cggcacggtc aggttcgtcc
     1501 tttggcgtaa ccaaagtaaa cggtacggaa gaacttaacg ctgcgataga agcggcagga
     1561 caatatgatg gaaaaatctt aattgagcaa gcgatttcgg gctgtgaggt cgggtgtgcg
     1621 gtcatgggga acgaggatga tttgattgtc ggcgaagtgg atcaaatccg gctgagccac
     1681 ggtatcttcc gcatccatca ggaaaacgag ccggaaaaag gctcagaaaa tgcgatgatt
     1741 acagttcacg cagacattcc ggtcgaggaa cgaaatcggg tgcaggaaac ggcaaagaaa
     1801 gtatatcggg tgcttggatg cagagggctt gcccgtgttg atcttttttt gcaggaggat
     1861 ggcggcatcg ttctaaatga ggtcaatacc atgcccggtt ttacatcgta cagccgctac
     1921 ccacgtatgg tggccgccgc aggaatcacg cttcctgcac tgattgacag cctgattaca
     1981 ttggcgttaa agaggtgata agtatggaaa atggtttttt gtttttagat gaaatgttgc
     2041 atggcgtccg ttgggatgcc aagtacgcca catgggataa cttcacagga aaaccggtgg
     2101 atgggtatga ggtgaatcgc atcatcggca caaaggccgt ggcgtttgct ctgcgcgagg
     2161 cacaaatcca tgcggctgcg cttggctatg gcttgctttt atgggatgga tatcggccaa
     2221 gaactgcggt ggactgcttc ctgcgttggg cagcgcaacc ggaggacaat ctcacaaaag
     2281 aaaaatttta ccccaatata gagcgagccg agttgattac aaagggttat gtggcctcac
     2341 aatccagcca tagccgtgga agcgcaattg atcttacgct ctaccacctg gatacagggg
     2401 aacttgtttc aatgggaagt aacttcgatt ttatggacga acggtcgcat catacagcaa
     2461 aagggatagg ggatgcagag gcacaaaatc gaagatgctt gcgtaaaatc atggaaagca
     2521 gcggatttca gtcttatcgc tttgaatggt ggcactataa gttgattgat gagccatacc
     2581 ccgataccta ttttaatttt gccgtttcat aatgaaagta ttggttttgt atcagatttt
     2641 ggatttgata aaatctatgc tggtcatgga caatctttta ctggaaagtg gttaaagcat
     2701 cttttgtaat acattgcaaa ttatagtatt caatctgccc agcggcagaa aagaaaaaaa
     2761 ccgctgttgg gcagaccttt cccacgccca ttttgtattt tgctggcggc ggttttgaga
     2821 aatcaaggcc gccgttcttt tctggcagca aaaggtgtgg cggcccatag gaggatgccg
     2881 ctatgctgtt ttgctattgc catgctctaa tccacttcca ccactcaaaa aagccttgcc
     2941 cctc
//
LOCUS       DACECY010000140         2920 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.148, whole genome shotgun sequence.
ACCESSION   DACECY010000140 DACECY010000000
VERSION     DACECY010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.148"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(14..>2920)
                     /locus_tag="IWO38_002814"
     rRNA            complement(14..>2920)
                     /locus_tag="IWO38_002814"
                     /product="23S ribosomal RNA"
BASE COUNT          612 a          880 c          600 g          828 t
ORIGIN      
        1 tttcttttaa cctattggtt aagtcctcga tcgattagta tcagtccgct ccatacatca
       61 ctgtacttcc actcctgacc tatctacctg atcatctctc agggatctta ctttcttaaa
      121 gaaatgggaa atctcatctt gaggtgggct tcacacttag atgctttcag cgtttatccc
      181 ttccctacat agctacccag caatgccctt ggcagaacaa ctggtacacc agcggtaagt
      241 ccatcccggt cctctcgtac taaggacagc tcctctcaaa tttccaacgc ccgcgacgga
      301 tagggaccga actgtctcac gacgttctga acccagctcg cgtgccgctt taatgggcga
      361 acagcccaac ccttgggacc gactacagcc ccaggatgcg acgagccgac atcgaggtgc
      421 caaacctccc cgtcgatgtg gactcttggg ggagataagc ctgttatccc cagggtagct
      481 tttatccgtt gagcgatggc ccttccatgc ggaaccaccg gatcactaag cccgactttc
      541 gtccctgctc gacttgttgg tctcgcagtc aagctccctt ctgcctttac actcttcgaa
      601 tgatttccaa ccattctgag ggaacctttg ggcgcctccg ttacctttta ggaggcgacc
      661 gccccagtca aactgcccat ctgacactgt ctcccaccac gattagtggt gcgggttaga
      721 gtggccataa cgcaggggta gtatcccacc agcgcctcca tcgaaactag cgttccgatt
      781 tctacggctc ctacctatcc tgtacatgcg gtacagacac tcaatatcaa actacagtaa
      841 agctccatgg ggtctttccg tcctgtcgcg ggtaacctgc atcttcacag gtactaaaat
      901 ttcaccgagt ctctcgttga gacagtgccc aaatcgttac gcctttcgtg cgggtcggaa
      961 cttacccgac aaggaatttc gctaccttag gaccgttata gttacggccg ccgtttactg
     1021 gggcttcaat tcgtaccttc gcttgcgcta agcactcctc ttaaccttcc agcaccgggc
     1081 aggcgtcagc ccctatactt catcttacga ttttgcagag acctgtgttt ttgataaaca
     1141 gtcgcttggg cctattcact gcggctgatc gtatgatcag caccccttct cccgaagtta
     1201 cggggtcatt ttgccgagtt ccttaacgag agttctctcg ctcaccttag gattctctcc
     1261 tcgactacct gtgtcggttt gcggtacggg ccgctgtttt ctcactagaa gcttttctcg
     1321 gcagtgtgac atcaggaact tcggtactat tatttccctc cccatcacag cttgtcctta
     1381 agaaaagaag catttgactc ctcttcagac ttactgcttg gacagacatt tccgatcgtc
     1441 tgcattcctt agcctcctgc gtccctccat tgctcaaaca aaaacaacgg gtacaggaat
     1501 atcaacctgt tatccatcgc ctacgcctgt cggcctcggc ttaggtcccg actaaccctg
     1561 ggcggacgag ccttccccag gaaaccttag tcattcggtg gacaggattc tcacctgtct
     1621 ttcgctactc ataccggcat tctcacttct aagcgctcca gcagtcctca cgatctgcct
     1681 tcaacgccct tagaacgctc tcctaccaat acacctaaag gtgtactcca cagcttcggt
     1741 aatatgttta gccccggtac attttcggcg cagggtcact cgactagtga gctattacgc
     1801 actctttaaa tggtggctgc ttctaagcca acatcctagt tgtctgtgca accccacatc
     1861 cttttccact taacatatat tttgggacct tagctggtgg tctgggctgt ttccctttcg
     1921 actatggatc ttatcactca cagtctgact cccggatatg aatgaatggc attcggagtt
     1981 tatctgaatt cggtaacccg agatgggccc ctagtccaaa cagtgctcta cctccatcat
     2041 tctcaattcc gaggctagcc ctaaagctat ttcggagaga accagctatc tccaagttcg
     2101 tttggaattt ctccgctacc cacacctcat ccccgcactt ttcaacgtac gtgggttcgg
     2161 tcctccagtg cgttttaccg caccttcaac ctggacatgg gtagatcaca tggtttcggg
     2221 tctacgacta catactcaaa cgccctattc agactcgctt tcgctgcggc tccgtctctt
     2281 caacttaacc tcgcatgcaa tcgtaactcg ccggttcatt ctacaaaagg cacgccatca
     2341 cccattaacg ggctttgact tgttgtaggc acacggtttc aggttctatt tcactcccct
     2401 tccggggtgc ttttcacctt tccctcacgg tactggttca ctatcggtca ctagggagta
     2461 tttagccttg ggagatggtc ctcccggatt ccgacggaat ttctcgtgtt ccgccgtact
     2521 caggatcctc ctaggtgtct tccacatttc gtctacgggg tttttaccct ctttgactga
     2581 cttttccaag tcattcgact atctgaaaga actaccatat tggagtccta caaccccaac
     2641 aagcaagctt gctggtttgg gcttttcccg tttcgctcgc cgctactcag ggaatcgaat
     2701 tttctttctc ttcctgcagg tacttagatg tttcagttct ctgcgtctac ctcgaatgtg
     2761 ctatgtattc acacaatcgt aacatcctat taaagatgtt gggttccccc attcggaaat
     2821 ctctggatca tagcttacgt acagctcccc aaagcatatc ggtgttagtc ccgtccttca
     2881 tcggctccta gtgccaaggc atccaccgtg cgcccttatt
//
LOCUS       DACECY010000141         2857 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.149, whole genome shotgun sequence.
ACCESSION   DACECY010000141 DACECY010000000
VERSION     DACECY010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.149"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            562..843
                     /locus_tag="IWO38_002815"
     CDS             562..843
                     /locus_tag="IWO38_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314827.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLEKFIAIQTKNTQVLNDYVHTNNQLIETLIGGK"
     gene            845..1261
                     /locus_tag="IWO38_002816"
     CDS             845..1261
                     /locus_tag="IWO38_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314828.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKT
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1422..1688)
                     /locus_tag="IWO38_002817"
     CDS             complement(1422..1688)
                     /locus_tag="IWO38_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314829.1"
                     /translation="MAFRFRCDRGGRKMTEKFPYEYYQNLEKLESAIQLVKLDLLDEN
                     LLKYKNITPERKGKLKKMQQLFDEEDGISDVIFDTWSEFHGEYN"
     gene            complement(1646..2620)
                     /locus_tag="IWO38_002818"
     CDS             complement(1646..2620)
                     /locus_tag="IWO38_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ0314830.1"
                     /translation="MGKKRTIVNEWEKLTAPSNRRLNVGESVKMIKMDWSIDRITIVG
                     TLNENIYYHTQNDVLILDFEQLMRVNEGNGHLKAVGNNGWQLVDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKKFIHDLFLEPHFSRADVACDLIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQEAKRKIVPKEVETWWRLELQ
                     LRRGKATDWYEMVQESLDSFASPHYFPEDVKTLDRVMIKGLLYDHSEWSTISRDLKYR
                     LRKLLKQESQNDELTNHLRETFEESADELKRELDTWLLGLDVTEEEEK"
BASE COUNT          926 a          624 c          397 g          910 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatcattt cacgcttccg ctcttcattt cattcagttc tcggctttca caatctaaca
      121 cgccgtttcg ctaccagtcg ccttacaggc tccattctcg cttctcttac gtgttttacc
      181 gattgtgccg acaactaaac gaaatgaacg ctaaaacgtc ttagaacgca aaatacacgc
      241 ttataaaatc atttcatctt gattatatgc tcatcataat cgttcaattt gacatcattt
      301 ccgtaaactt tttttatttt tataagcaga aaaaagtcgc acatcaacat ttttttaaag
      361 agcataagga aacgtgtacc cactttctaa aaaaagcggg cagataggct gtgcctatca
      421 cgtttctaag aaaaaaattt gatatcactt tttcgccatt gtgactagct tatgtatagt
      481 gtttcagcaa aaaacagcta tacaaatact atattcgtat gatatacata aattatacac
      541 aaatgcgaaa ggagtttttc tatggacatt ttagttcgga agattgattc aaaggttatt
      601 gcaaaaatag acaaacgttg ccaagaactc acaaataaaa ctggcaaaaa atggagtcga
      661 aataaatatc taaaagtttt aatcgaaagt gattttgacc gtcaacttac gacttacaaa
      721 aaagaccaat tcgatttatt actcgaaaaa tttatcgcta ttcagacaaa aaacactcaa
      781 gtactaaacg actatgttca tacaaataat caacttattg aaacacttat tggaggaaaa
      841 tagcatgaga aaaacttctg ttcaaaaaca cgttaccctt tatcccagtc acgtggacgt
      901 tttagattcc attatcgaaa gaaatccaga tatcaacaat tactcggaag ccattcgttt
      961 tttatgtatg agttatgaac ctgataccaa cacgactact accagcaatc aagccaaact
     1021 aaatgctatg ggaaaagaaa tttccatcat gtccgaaatc attgcagaat ttgctgattt
     1081 atatctaaaa gatacaggcg ttcttattgg cacaaattct aaagtgtatc aagaagtgaa
     1141 agaaaaaacc gaagctaaac taaatccgtc gaatcgaaat cgaccaaaga atcatcaaaa
     1201 agaaacgagt caaacagtca cttctagaaa tttcgaccca acaaattttc taaaacgcta
     1261 aaaaataaaa cgagaatttt tttatttctc gttttatttt ttaggaaagc cggtcttaca
     1321 gcaagatttg caagttaccg gtaacttgcg gaaaagaaga agccgctcac gcgacttctt
     1381 ctttttgcat tttctgaaag ctaccacaat tcctaacttc gctaattata ttctccatga
     1441 aattcgctcc atgtatcaaa aatcacatct gaaattccat cttcttcatc aaataattgc
     1501 tgcatttttt tcaatttacc ctttctctct ggcgtaatat ttttgtattt tagtaaattc
     1561 tcatccaaca aatccagctt gaccaactga atagcactct ccaatttttc tagattttga
     1621 taatactcgt atggaaattt ctcagtcatt tttcttcctc ctctgtcaca tctaaaccta
     1681 aaagccatgt atccaattct ctttttaatt cgtctgcaga ctcctcaaat gtttctcgta
     1741 aatgattcgt caattcatca ttttgacttt cttgcttcag caatttcctt agccgatatt
     1801 tcaagtcacg actaatagtt gaccactcag aatggtcata caacaacccc tttatcatca
     1861 ctctatccaa cgtttttaca tcttcaggaa aataatgcgg actagcaaaa ctatccaaac
     1921 tttcttgaac catctcatac caatcagtcg cttttccacg gcgcaattgt aactccaaac
     1981 gccaccacgt ctcgacttct ttaggtacaa tttttcgctt tgcttcctgt tccaacttct
     2041 tgttgtacat ccgaatttgg cgttcgctgg cacgactacc ccaataagcc gtttctagct
     2101 taccagagcg tccataaatt ggtttgaagg acacagggtc aactacacga tattgtgtaa
     2161 taaactcatc aggcacatca ataagatcgc aggcaacatc cgctcgtgaa aaatggggct
     2221 ctaaaaataa atcatgaata aattttttca tagagcttgc taaaaattga ctaattttat
     2281 tggggttaaa gtcaatccgc cctttaccct cttgccattt caaaatttcg atataggcaa
     2341 tattttcttc gtgttgatct accaattgcc aaccattatt cccaacagct tttaaatgac
     2401 cattaccttc gttcactcgc attaattgct caaaatctaa aatcaacaca tcattttgag
     2461 tgtgataata aatattttca ttcaaagttc cgacaatcgt aattcggtca atcgaccaat
     2521 ccatttttat catttttacg ctctcaccga catttaaccg cctgttagag ggggctgtta
     2581 atttttccca ctcattcaca attgttcttt ttttacccaa attttgttcc tcctcaaaga
     2641 aaaaaagaga tttttggaaa gaatttccag agttcacgta actctaaaaa tccacatcca
     2701 aaaatctcta gcttgaagaa acctaaaaat attcgtataa tgaacttgag ttacggaact
     2761 aggctagtag tttccgtaaa ggtcgagagt gttagcgcac tgctcgacta tttttttgct
     2821 cttttttctc gttttctttg attatagcac tcctctt
//
LOCUS       DACECY010000142         2731 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.150, whole genome shotgun sequence.
ACCESSION   DACECY010000142 DACECY010000000
VERSION     DACECY010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.150"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..334
                     /locus_tag="IWO38_002819"
     CDS             <1..334
                     /locus_tag="IWO38_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ0314831.1"
                     /translation="KLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKK
                     TGGLRYAPALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKRED
                     FNEAQKIR"
     gene            350..1111
                     /locus_tag="IWO38_002820"
     CDS             350..1111
                     /locus_tag="IWO38_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ0314832.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            1242..2576
                     /locus_tag="IWO38_002821"
     CDS             1242..2576
                     /locus_tag="IWO38_002821"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ0314833.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
BASE COUNT          926 a          463 c          585 g          757 t
ORIGIN      
        1 gaagctattt gaagagtcag acgaagagac gattgcagga atcatcaaac atgaactttg
       61 ccattatcat cttcatctag ctggaagagg gtatcagcat aaagatatgg atttcaagta
      121 tcttttgaaa aaaacaggag ggttgcgcta tgcaccagcc ttgacgaaga aacagataaa
      181 gatggagtat tatcgatgcc aagattgttc tgccacgatc tgccgcaaga gaagaatcga
      241 tgtacgcaaa tatcgttgtg gaagatgtca tggagaaatc aaatggattg agacaaaaag
      301 agaagacttt aatgaagcac aaaaaatcag atgaaaggat agaaggcctt tggaaaaaag
      361 tgagcagagg aatctaaaag actggaatca gctattaaat tttttagccg cacctgaaga
      421 gggaaatttt gaggaactga cggcacctac acttatttta gctggaaact gtcttcctat
      481 cttagctgat aaagcagcac aaatgtattt aaatggccaa gtagatcaat tgtttttggt
      541 cggaggggtg gggcatgcga ctagaatcct ttatgagaat tttgaaaagc aaggatttca
      601 ttttgaagat ggcatgagtg aaagtgaaat ctgtcgtcag tatctaaaag aagtctatga
      661 tttgcccgat aaggcttttc tgatagaatc gaagtctaca aattctggag agaacgccac
      721 tttttcactg gagattcttc attccttagg agctgtacca gaaaaaatct tattgatgaa
      781 tgatccgaca ctgcaacgca ggactcgagc aacttttgag aaagtatggc aaaatgaaca
      841 aactattttc gcgaatgctg tgccatttgt gccggaaatc cttcactttt ctgaagagat
      901 catctttaca gcaaaagaac tgaatcatca atggcctaag gaatatttcc atgcgctagt
      961 attaggagaa atggaacgtc tccatgacga tgagaacggc tatggtccga aaggaaaaga
     1021 ttttattcct cacattgata tatcagagga agtttggtct tcttacacca gaattaaaga
     1081 gtcaataaaa acggattttt ctcgtacttg accattttag caagcgaatc ctcctttcca
     1141 aataaatttg gttgaaattt tcaatcgttt ataagataat gaggagcgtg ttgaatgtaa
     1201 tagctaaaag aatggacgct agataagaga agaggattca aatggaaaat caaaataaat
     1261 tgtatcattt tgttggtatc aaaggttcag gaatgagctc cttggctctt gttttacatg
     1321 aacaaggtct aaatgtccaa ggatcagaca ttgaaaaata tttctttaca caaagagatt
     1381 tagaaaaagc aaatatcacg attctgccat ttaacgcaga taatgtaaaa ccagggatga
     1441 cgatcattgc aggaaatgca tttcctgatt cacatgaaga aatccagcga gcaaaagaat
     1501 taggtttgga agtgattcgc tatcacgact ttattggtca ttttatccaa aattatacga
     1561 gtatcgctgt aacaggctct cacggaaaaa caagtacgac tggactgctc tctcatgtat
     1621 tgagtggtgt ccgtccaaca agttatctaa ttggagatgg aacaggacat ggtgatccgc
     1681 aagcggaatt cttttcattt gaggcctgtg aatatcgccg tcatttcctt gcctattcac
     1741 cagactatgc aatcatgacc aatatcgatt ttgatcatcc ggattattat acaagtatcg
     1801 acgatgtatt tacagctttc caaacaatgg ctggacaagt gaaaaaagct atttttgcgt
     1861 atggtgacga tgcttattta cgcaaattaa aagcaaatgt gccgatttat tattatggtg
     1921 tcacagaaaa cgatgatatc caagcacgaa acatcgaacg cacaacttct ggttctgctt
     1981 ttgatgtcta tcatggggat gaatttgttg ggcacttcac tgtaccagca ttcggtaaac
     2041 acaatatctt aaatgcttta ggtgttattg cagtagctta tttcgaaaaa ctggatctga
     2101 aagaagttgc agaagaaatg ctgactttcc caggagtaaa acgtcgattc agtgaaaaga
     2161 tcgtagcaga catgaccgta gtcgatgatt atgcgcatca ccctgcagag atcaaagcaa
     2221 cgattgatgg cgcacgccaa aaatatccag ataaagaaat aattgcagtt ttccaacccc
     2281 atacgtttac gcgtacgatt gcattgatgg atgaatttgc agaagcactt gatttagctg
     2341 ataaagttta cttatgcgat atttttggtt cggctcggga agaacaagga aacgttaaga
     2401 ttgaagacct tggagcgaaa atcaaaaaag gtggagaagt catcaaagaa aataatgtat
     2461 ctccattgtt agattatcat gatgcagtag taatcttcat gggtgctgga gatgttcaaa
     2521 aatttgaaca agcgtatgaa aaacttttaa gcagcacgac gaaaaatgta ctgtaataca
     2581 attactgcag tgtccttata aaaatagaaa gagggggagt ggcaaaaatc ttgccaagcc
     2641 cccttttttg caacttatga taaacggtgt tcaaaagctt gcatctgcgg taataagccc
     2701 aatcaaatca aaaacatgag aagctgtttt t
//
LOCUS       DACECY010000143         2719 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.151, whole genome shotgun sequence.
ACCESSION   DACECY010000143 DACECY010000000
VERSION     DACECY010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.151"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="IWO38_002822"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="IWO38_002822"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0314834.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1065 a          420 c          556 g          678 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatca
//
LOCUS       DACECY010000144         2647 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.152, whole genome shotgun sequence.
ACCESSION   DACECY010000144 DACECY010000000
VERSION     DACECY010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.152"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1..344)
                     /locus_tag="IWO38_002823"
                     /pseudo
     CDS             complement(1..344)
                     /locus_tag="IWO38_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(418..699)
                     /locus_tag="IWO38_002824"
                     /pseudo
     CDS             complement(418..699)
                     /locus_tag="IWO38_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1161..2543
                     /locus_tag="IWO38_002825"
                     /pseudo
     CDS             1161..2543
                     /locus_tag="IWO38_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          946 a          461 c          440 g          800 t
ORIGIN      
        1 gcataggctg cttgtgtgtc ccgtttttta cgtaaccaga tatctaaggt taagacgtcc
       61 gcatcaattg cacgataaag ataatgccaa cgtcccttaa ttttgatata ggtttcgtcc
      121 attttccatg aatagaagga ttgtctattt ttcttcttcc aaagataata gaggactttg
      181 ctgtactctt atacccaacg ataaatcgta gtatgacaaa catttattcc acgatcatat
      241 aacaattcct gaacttcacg atagcttaga ttgtaacgca ggtagtaacc aacagcgaca
      301 ataatgacgt cttttttgaa ttgtttgcct ttaaaatgat tcattactct gccctctctg
      361 tcttttttct caattttaca ctaaaataga ttttttggaa aactttgcaa cagaaccgaa
      421 atgagtatag tatgaattag taatttggtt ttcatctaag tttagctttg atttactaaa
      481 attgatatct cgatacatgt tcaatacatc taatacaaat cgagaatctt ctttcggaaa
      541 tggctcagaa agatcactcc aaatttcatc atcataatga tactcgtaac cttctctcaa
      601 tatttcgatt tttctttcat aataagatct gttttggtcc tcaaatttag ccaataactc
      661 aaattgattt actagtgaca atctctgaaa actgtccatt aaatgtttct tcctatcttg
      721 ttcattaaca aaaaattata agcccttaca acgaatatta tatattaaat ttaattgtag
      781 gtgaacacga attttataaa tatttatggt gttaatgaca aatgatatcc gactcctctt
      841 catacgttct ggactatttg ctaatagatt aaaatttcag catctatttc tgaccataaa
      901 taaatgagct gccgaaaagc tgccaaatca gacttgaaaa taaaggatca aaaactttta
      961 agtttctgac cccgtaactc acacttcaaa atcttactta aaaaggagtc tgaaagaaaa
     1021 aaagagtatt caaaattttt ttctaactag ctacatcaca tctatgcatt gacactcaaa
     1081 gaaccagaat aagtcatcag aaagcaaggg ggtgcggaca taatcctgga cgaaaaaata
     1141 aacttaggag gaatgtccgt gtgttttccc acaaagagcg tgtaagggca attcagttat
     1201 ttttaaaata cgattgctct tatgctgcaa ccattcgaga attagggtat ccgtcgattg
     1261 gtgcactacg gaaatggtat aaagagtatt tggaatccgg agagctccat caggagcacc
     1321 gaaaaaaatc aaagtattct gaagaacaaa aacgaatagc ggtgaatcat tattttgaat
     1381 atggtcaatg ttatgcacga accattcgca tgcttggtta tcctaataga gagtccttgc
     1441 ggcagcggtg tgaagaactt gcaccaggag ctagaaaact ccgaaaaagt gcggtaaagt
     1501 taacacaaga tcaaaaagaa gctgtgttga aaaaattcta taaaccgcaa actaatagga
     1561 aaaatttagc cgaatctgga ggaattagta gagttactct atatcaatgg aaaaacacgg
     1621 ttcttggaaa ggattttcca ttgtgtatga ctttaaaaaa tcaggaaaaa caaaaagagc
     1681 tacttttaaa ggaagtagaa gacttacaaa aacaagtaca ccaattacag ctcgaaaaag
     1741 cattactcga aggagcggct gaactgctaa aaaaagaaaa aggcgtcaat cttctttgtc
     1801 tatcaaatca agaaaaaacg atattgattg atgccttacg aaatcagttc accttgaaag
     1861 agcttcttca acagctccag tttcctaaaa gcagctattt ttatcaaaaa caagcacttg
     1921 agaaaccaga taagtattac aaagaacgtc aaattaatta tcacgatttt caatcataac
     1981 ttctgtgcgt atggttaccg tagaattcac caagctttaa aaaatatggg taaaaagctt
     2041 tctgaaaaag ttgtacgtag attgatgact gaggaaaatc tatttgttaa attttctaga
     2101 cgaaaaaagt attcttcgta tgctggggaa atttctcctg cacaccccaa tttattaaat
     2161 cggaatttta aagcaaagaa gccaaatgaa aaatggttaa cagatattac tgaatttaga
     2221 attctcgcag ggaaaatcta cttatcacca cttgtcgatt gttacgatgg tgctattgtt
     2281 agttggacta ttggaacaaa gccaacttgc ttcactctat gtctaaaaag agttattcac
     2341 cagataattc agcgtgcgaa ggtttctttg gacgattaaa aaacgagatg ttttatcaac
     2401 gtgactggaa gaatacaaca atcaatcagt ttataaacaa attggatgat tatattcatt
     2461 ggtataataa tcaacgaatt aagttatctt taggaaggct aagccctctt caatatcgga
     2521 agaagcaaag atatatttgt taaaaaggtc caaaaaaaat atccgcaccc cctttttttt
     2581 gtaaaaaact attgcacaat acaagtagcc tacggctatc gaaatttcca taactttcga
     2641 gcaagaa
//
LOCUS       DACECY010000145         2634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.153, whole genome shotgun sequence.
ACCESSION   DACECY010000145 DACECY010000000
VERSION     DACECY010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.153"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            5..1150
                     /locus_tag="IWO38_002826"
     CDS             5..1150
                     /locus_tag="IWO38_002826"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAQ0314835.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="IWO38_002827"
     CDS             1321..1779
                     /locus_tag="IWO38_002827"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ0314836.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="IWO38_002828"
     CDS             1938..2408
                     /locus_tag="IWO38_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ0314837.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(2461..2576)
                     /gene="rrf"
                     /locus_tag="IWO38_002829"
     rRNA            complement(2461..2576)
                     /gene="rrf"
                     /locus_tag="IWO38_002829"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          862 a          505 c          496 g          771 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaat aaaaaaagat gaactatttc tagttcatct ttttgccttt
     2461 gcgtggcgac gtcctactct cacaaggggc accccctcac tacaatcggc gctaagaagc
     2521 ttaacttctg tgttcggcat ggttacaggt gtatccttct cgctatcgcc accacactgt
     2581 ggtgttatct tttattgagt aaattttgtt cactcaaaac tggatttgaa gtca
//
LOCUS       DACECY010000146         2484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.156, whole genome shotgun sequence.
ACCESSION   DACECY010000146 DACECY010000000
VERSION     DACECY010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.156"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1014..1304)
                     /locus_tag="IWO38_002830"
     CDS             complement(1014..1304)
                     /locus_tag="IWO38_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314838.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2204)
                     /locus_tag="IWO38_002831"
     CDS             complement(1512..2204)
                     /locus_tag="IWO38_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ0314839.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          734 a          458 c          349 g          943 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgagtttc tacatttgtt
     1681 ttttcttgtg tcagtgttgc gatttgttga ttcaaaccat ttacagtatt ttgtaattct
     1741 gaaagttttg cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag atgattgatt
     1801 tcatcatttt tttggttgat ctcatctagt ttgacattga tttcattttg tttgctttgt
     1861 agttgtgcat ccttgctgac taattgactt tctaagtttt gtttttctgc tgccgattga
     1921 tttttttggt tttctaattc atttactttt gtttggtatt cttggacatc tgcctttgaa
     1981 tcttgcaatg tctgttcttt ttgtgctagt cgagcggtaa gatctgtaat cgcttgctct
     2041 ttatctgtca atgtatgatc aagctgtgtc agattctcat tgattgtatc aatatctgtc
     2101 tctccacccc acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat
     2161 agaaatgcaa cgacaatccc accagtaata aatttctttt tcatttgcat agctccttaa
     2221 tccaatactt ttacatgcta aaagtaagtg atctcaaaca caatgtcaag cgtttttaaa
     2281 ttacatatgt aattttaata taaatactat caaatactca tttaatcctc cttttttatc
     2341 tatttaaaaa atctttttta tagagaaaaa tgcaggttaa tactttctta aataaatatt
     2401 tatggtgaac aaaaacaaaa aagcaagttt tattaaacgt aacccttttc atttcataga
     2461 ttactgtatt tattacaaaa aacg
//
LOCUS       DACECY010000147         2468 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.157, whole genome shotgun sequence.
ACCESSION   DACECY010000147 DACECY010000000
VERSION     DACECY010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.157"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(493..1785)
                     /locus_tag="IWO38_002832"
     CDS             complement(493..1785)
                     /locus_tag="IWO38_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0314840.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(2059..2319)
                     /locus_tag="IWO38_002833"
     CDS             complement(2059..2319)
                     /locus_tag="IWO38_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314841.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          823 a          459 c          372 g          814 t
ORIGIN      
        1 ttatattata aattcacatg attatcgaat tttattaagt tagtttttct tgtaaatctc
       61 tgttcccaat gaaactcgag acacttattt taaagaggaa acaatgtggt atttaaatag
      121 ccctcttgta tgggctactc tgtgatactt attagccatt aaactatttt acccacatag
      181 catatatttt gaaatcgttc attccgtaaa ttcaactaaa tatgcctaaa aaaataaaaa
      241 atctaagata gaataagatt tattcagtct tagatggatt tatagcaact ttttcgtaac
      301 aactttaaaa aatacgaaaa gaaagagctg ttttactctg actacgttgc taaattgtcg
      361 tttaatcatt catttaaacc aaatcttttt ttatagaatt cgttttaatc ctcctgaatc
      421 caaaaactat gaaaaccgta attaaatagt taagagtttt taaccaccat aattctctcg
      481 gctattttga aatcagataa attttgatcg gacacatagt tgaataaacc taccgttgtt
      541 acggaaggta aatcgcatac ttttcattct agaggagaag gtctcgttga tcattaactg
      601 attaagcacc atagcccagt tttgaattcg accacctgac cacttcgcgt gtaattcttt
      661 cgtgcgtaaa taaagtagtt ttaggagtgc attctcatta gagaacgctc ctttttttgt
      721 gacttttctg aagctggagt ggacactttc aaccgcattg gtagtgtaca taatttttcg
      781 aatggcacta ccataatcaa atagttgttc aacatgtgca aagttccgtt tccagacatc
      841 tacagcacca gaataatgag accaccgatt ttgaaaactg tcaaaagcag catgtgcagc
      901 gtttagagaa gaagcaccgt agaacttttt catatctcgg cagacttcct tatagtcctt
      961 acttggaata tagcgcaatg catttcgaac aagatgaaca atacagcgct gaacaattac
     1021 cgatggaaag atcgcttttg cgccttcttc gaggccagaa acaccgtcca tcgaaatgaa
     1081 aaagacatct tcgacaccac gtgctttcag ttcatcaaat acttgcatcc agcgattttt
     1141 agattctgtt tgatttaacc ataatcctaa aatctcctta tttcctttga gatcatagcc
     1201 aagaatggtg tatacagcat attctttggc ttcataattt tctcgtaaag taacatacat
     1261 acaatcaacg aatagaaagg cataacactt tgctaggggg cgggcttgcc attcttccaa
     1321 ttcaggaagg acagcgtcag tgatatctga aatcatttca tgggaaatat caaagccata
     1381 aatagcttcg acggttgcgg caatatctcg ttgactcatt cctcgtgcat acatggaaag
     1441 aaccttccct tcgatgtcgg agacatctcg ttttctctta ggaattaact ctggttcaaa
     1501 ggaagcttcc cggtctctag gaacatcaat agctacttca ccaaaactgg ttttaagcgt
     1561 tttagttcca tagccatttc gacggttatc gtgttcctta ggctctttag aatgggcatc
     1621 ataacctaaa tgattattca attctccttg aagcattttt tcaaaaaggg gcccaaacac
     1681 atctttcaaa gcatcttgca tgtcatcgac agattcaggt tgataggcat tcagaatgga
     1741 ttcagctaac ttttcggcat caggatttct tttctttcta gccatcgtgt aatcacctct
     1801 tgatcttatt gtagaaaaag aaaaaccgcg aagcaaccac ctgcctagga ttaatggtta
     1861 cttacacggt ttatattaca ctctcatcat taaaaaagta tctttcgata ttactaaaca
     1921 atgaaattat ctatttgaaa tttaaatctg ataatatatt gagtgttcca atgagggctt
     1981 tgatcctatt atccatatct ttttcagttt taatattgat acacattata agaaaaagaa
     2041 acttttggtt aatttatttc atcccagtgc aaaatataaa aaagtaaaat agaccatagg
     2101 caatagatta acaattaata taatacaagc aaatattcta ttcattgtat ttctgctttc
     2161 aaaaagttct aatattgaaa gtataattcc acaaatgcct ataataagta tgggaataag
     2221 attagaaaat aacaccccga gccaatctgt gaaaattact atatttacta atatccctat
     2281 taacatacct atcatattta ttaaatcaat ttttttcatt agatatttct cctttattaa
     2341 atctgaagta cttatattaa aataattcta aactatttac ctcaaaaagt gcaataaatt
     2401 atttctgaac aataaaatga tagtcaaaac aaaaaaatat tgttaatcgt tcagagccat
     2461 ccgagagt
//
LOCUS       DACECY010000148         2464 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.158, whole genome shotgun sequence.
ACCESSION   DACECY010000148 DACECY010000000
VERSION     DACECY010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.158"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(48..596)
                     /locus_tag="IWO38_002834"
     CDS             complement(48..596)
                     /locus_tag="IWO38_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314842.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(597..1454)
                     /locus_tag="IWO38_002835"
     CDS             complement(597..1454)
                     /locus_tag="IWO38_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0314843.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(1740..2027)
                     /locus_tag="IWO38_002836"
     CDS             complement(1740..2027)
                     /locus_tag="IWO38_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0314844.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSVIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHEKFYEQLKKLI"
     gene            complement(2017..2346)
                     /locus_tag="IWO38_002837"
     CDS             complement(2017..2346)
                     /locus_tag="IWO38_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0314845.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          717 a          488 c          329 g          930 t
ORIGIN      
        1 ctaagtaact accgtttaaa ggtagttact tagttcttct ttgattatta tctaacttta
       61 ttattcattc cccacccttt gcgcagtgag tcttcggtat cttgttttat ctgactggta
      121 gttcttatta tttctccacg atcatctaat tctggaacaa ttggatattt ttctctgaac
      181 aagtctagtt ctttcaccac gtcattaaac aaatcttgct tatcttcttg cgtaagattc
      241 tgtttaatat actcatctaa agcttttcta agcaattcat ctcttgtcat attagtctca
      301 tacttttcta ctaaaactct ggaagtcgca tttaacaaag tcgacagttt taaaggatgc
      361 ttggtggaat aaaccttatc aaactttctg attggtgcgc caactttttt tgttcttcct
      421 ctactctgtc tttcaacatt ggaggaagtt tttatttctt gaacttggtg cacttctaat
      481 tgcttatttt cagatggtgt aattgcttgc tgctttttct ttgcttctgc ttcttttttt
      541 attttggcga gtcctttatt gttcccaaaa atatcaagtc ctttgatgtc agccaattac
      601 tccacctccg cataccaatt ctcgtgttct tccaattctt tcaaaatact gataaataat
      661 tcgtggcttt tgcgatccca ttgttttaag agacctttct ttgtcttctc catcgtaata
      721 cctgatccgt cgtatcgttt taagcgcgca tttttgagta caacattttt aatcaaatgt
      781 tccccataaa tctcttgggc ttgtttatac atttcctgat ctactgcgtc accgctttcc
      841 aacatgaaag gaataacacc agcaacatat aaatttgtat caaattcttt cacaaaaaag
      901 tccatatact caagatattg catcgcattt ctcatcgatt ttacttgggt ctgtaagacg
      961 acgattgccc agtccgcaat gtacatcgcg gatttactat atacagaaat agaaggtggg
     1021 acatctaaat agatgacgtc aaactcatca aataatggtt ctagcttttc tttcatatat
     1081 ttgaattggg ataatgaatc attatattgg tctttgatcc aatcatcata catttcaaag
     1141 gacatgtctg ctggaagtat atagaggtta ttgattactt tatagacagc ctctttaggt
     1201 ggaagattgt ttatcaacac gtcagtcaca gtattttcgt aaacaatcgg ttcaccttct
     1261 ccgcctcctt cttcgttagt gaagttcata tgcgttaagt tcatcacatc agaagtattt
     1321 ccttgcatat ccaagtcaat tatcaaggtt ctttttcctt cattaactgc tgattcaaac
     1381 cctaacattt gcgcgcacgt acttttacct acgccacctt taaaattacc gattacatat
     1441 ttcttcgcta ccacgttcct acctcttttc ttttagcaat attcatttga tactattata
     1501 acatatttat ttttagttac taccaaaaaa aagatatata tgtactacct tataattaat
     1561 aaggaaaagg tagtacataa ataaggaaaa gctgaaatat attgatttat agctttcctc
     1621 aaagtaatga cgttatttaa tttatcgaga agcagagaat actcgcgact ttagtcatgt
     1681 agacgaatca gattttcgat aaaaaagaag ccgtccttct taagaacgac aacaaatcac
     1741 tagattaatt ttttcaactg ttcataaaat ttttcatgcg ttccacataa caatactgga
     1801 ataaccttgt tttctataat ctcgtaagct actctatatg ttgtgcccgc atatttaaac
     1861 ccactagacc aaataactga taaatcccca gttttttgtt cacccttata tggatcaatc
     1921 gcaatttcat cataaattaa attcaaaaat ttttctttca atggcttctc ttttagcttt
     1981 tttaaatact taatgacatg ttttgaagga acgatttcat aagccaattt ctgttgccaa
     2041 ctcctcttta gtcattactt tagaattagt aagtgtatct tcagcaatat ctctaaagga
     2101 agaatgcatt ttgttttttc tattcttaaa ttctacaaga atctcatctt tatcgtagcc
     2161 ttctgcaatt atatcagaca aaatatcttc gctaaaatca taaaattctt tttgtggttc
     2221 aacaaattca taaagaatac cattctctac caactttgct tcaacttcga ctccattagg
     2281 aacattaaaa tctttaggta ctgtcaacat gattgaattg ccttgttttc ttgtttttgt
     2341 aatcatttaa atccctcatt tctaacagta tgtaattact tagtaaatac attataccac
     2401 gagataatgt atttacaagt aattactgtg actcgtaatg cgtccttgct gaataaattt
     2461 caac
//
LOCUS       DACECY010000149         2400 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.159, whole genome shotgun sequence.
ACCESSION   DACECY010000149 DACECY010000000
VERSION     DACECY010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.159"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            209..2071
                     /locus_tag="IWO38_002838"
     CDS             209..2071
                     /locus_tag="IWO38_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0314846.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2071..>2400
                     /locus_tag="IWO38_002839"
     CDS             2071..>2400
                     /locus_tag="IWO38_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0314847.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 taaaaaattt gaaaaagcgt ttgattattt atattacgac ttagtgcaat aaaaaacgct
       61 ttttaacagt acatgtagtc gtctttataa tagataaact ctgtaatatt agttaaaaag
      121 ctataagttg cacttagtta agtgcaaaca tttcatattg tcgttagttt agaaaacgct
      181 tatattagca atgtaaggag agatgataat gcagcagcta acaaaaagag aaaggcagct
      241 tttgatttac ttggtggaaa tgtctgaacc ggtaacaatc aaaaagttgg ctgaaaaaga
      301 aaatgttagt gttcggacga taaaatacga tctagatgat ataagagagt ggctgaatga
      361 gcggaagcaa gtcctatata gcaaacgtag tcagggaatt tggatgaata ttagtagctc
      421 tgatcgaatc aaattaaaaa gcgaattgat ggatgttaat cgttttgaaa tatttgcaga
      481 tcaaagtctc cgaatcaaac gtttactaat tatgctttta gtaacaaaag aagctattac
      541 tggaatgaaa atggccaacg cattagatgt cagcaaagat acgatcatga atgatttaga
      601 tgtgctggaa aataattttc ttaaggaagg attgaccttg aatcgtcagg cgcgaaaggg
      661 tttttggatc ataggagaag aacgtctagt tcgattaacg attgaagaga ttcttcaaaa
      721 agagttcacg gattatgaca tttataaatt aatgtcattg ttattgaatg gcggtgagac
      781 tgaatacttt gaaatgtatt ctgctactgc tacacctatc caagaggtat ttaatcaagt
      841 aatcattcga atgcgacact tactggaatt tgaaaatcta gagaaactga attacgcgga
      901 attactaaat attctgattc gagtgacaat tgctaccgtt cgcttacgca aagaggctac
      961 gattggtcgt tatcaattgg ttgctgaaca agaattagac aaaaacgatc tttcttatcg
     1021 cgtcatgcag caggtctttg attattatca gttgccactg ttcgaggatg aatatcatta
     1081 tatctatagt gatacattcg acacgattca ccagcaagac gtcatgaaac tagttgagaa
     1141 tgtgatttat aaagtaagta aagcaatcaa ccattctttt tatcaagatc cacaattgtt
     1201 gacgaatctg tatgctcatt tatccatgcg attatcgaga aagaaaaaat ttgtcaatga
     1261 atataatcct tttaaagaag atatcaagca gaaatacccg gaattatttg atgcgatcaa
     1321 gcaagcagtt cgacaagaaa taagtgaaca aaatattctg atcaatgatt ctttcgtggc
     1381 atatattgcc ttacactttt tggtttccta cgaaagagaa gaagatctac gaagcgttag
     1441 agccgtctac atttgttcaa cagggttggg tgtgactagt ctgatcaagc aaaagatcaa
     1501 tgaagaatta aataatatca ctatcgtggc atttgcttca gtattgaatg cgcaagaaat
     1561 gattgctacc aaagatcctg atttggtgat tagtattttt ccaattaatg gcatcgatcg
     1621 tcctttcatc aaagtccatc cattacccac tgaacaggac ttgaaaaaaa ttcaacaaat
     1681 aataaaaaaa atacttgctt cttccaatca gcaggaaagg cataacttga atcttagaga
     1741 aacgtccatc aataaagata ctttagaaga ctttagcaag gaattgattt tgaaagctta
     1801 tacgatttat gaacgtttac ttgtcatttt ggaagcagaa ttgatccaag aatatcgaga
     1861 agcttttttg cttcatgtat tcttaatggt tcatcgtatc acatttgatc aacaatatat
     1921 ggcagaaggt cgtcaagtag accagattat tttagacaaa ggaacactgg taggaaaaat
     1981 cgaagcagtt tttgcagaaa ataatttagg aatcaatcag tcagaaatca gtgcattatt
     2041 ctcatatata aaagaaggag cgtcaagtta gtgaaaatgt cagacgaggc tttaaaaatt
     2101 atcgaggaaa gtcaaaatcg ggaaaagtta aaacaagtga ttgatctaat tgaagagcga
     2161 ttagtagaac atgctattgt accaaccgag ttgcagtgga ccattctaat taatcacttg
     2221 aatgagatgc tgaaacgttc tcaaagaaag gaaacgattc ccgtagtcga tcgggatttg
     2281 ttcaaagaag tatccaaaga agccataact atttctgatg aaatagttag aagtatcggt
     2341 aatttaaccg atgatgaaat atatgttttg tcgattcatt ttgaaacagc aaaaaataat
//
LOCUS       DACECY010000150         2360 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.160, whole genome shotgun sequence.
ACCESSION   DACECY010000150 DACECY010000000
VERSION     DACECY010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.160"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            335..1660
                     /locus_tag="IWO38_002840"
     CDS             335..1660
                     /locus_tag="IWO38_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ0314848.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRANDLPYPLPKELYIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLFTSSETRSERRRELAKIIKDEIEKETGLIA
                     TVGIGDNPLLAKLALDNEAKSSKEFIAEWTYETVPEKIWKINEMTDFWGIGSRMKKRL
                     NHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKI
                     PITDNTKELQNYCLFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEEN
                     LDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            1653..2000
                     /locus_tag="IWO38_002841"
     CDS             1653..2000
                     /locus_tag="IWO38_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314849.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFAELLKWSQI"
BASE COUNT          876 a          328 c          456 g          700 t
ORIGIN      
        1 gaaccacata aaacagcatg tttctcaaat tgccttagtg gttgaattga gatctatcac
       61 gttttagaac catataaaac aacatagttc tcaaattgtt taaaatgatt gtcaagttaa
      121 aaaaatctgg gatcatataa aatagtatag ttctcaaata tgattagaaa ccctattaaa
      181 gagcaatatt aaaccaactt acttaaatcg tttaattaag aacagtaatc tcttttagtc
      241 agacttttag tctgactttt ttgtatttcc aaaatttatt ctttacaaaa gcgaacgtac
      301 gtttgtataa ttgcttgtga atgggggaat aaaaatgttt gattacagta gagaaccggt
      361 gaatgactat tttttaatcg acatgaagtc tttctatgct agcgtggaat gtattgaacg
      421 aaatttagac ccaatgacta caccgttaat tgtcatgagt agatcggata acactggttc
      481 aggtctgact ctagcttctt cacctactgc aaagaaaaaa tatcatatca ctaatgttag
      541 tagagctaat gatttacctt atccattacc aaaagaactt tatattgttc cacctcgaat
      601 gaacttatat ataaaaaaaa atatggaagt taatcagatt tttagaaagt atgtcgcaga
      661 tgaagattta ttgatttatt cgattgatga atcaatttta aaagtgacaa aatccttaaa
      721 tttatttact agttcagaaa caagaagcga gaggagaaga gaattagcta aaataatcaa
      781 agatgagatt gagaaagaaa caggactaat agcaacagtc ggaataggag acaacccatt
      841 attagctaaa ctcgcactgg acaatgaagc gaagagtagt aaagaattta tagctgagtg
      901 gacttatgag actgtacctg aaaaaatatg gaaaataaac gagatgactg acttttgggg
      961 cattggatca cgcatgaaaa aaagattgaa tcatatggga attatgagta tagaagattt
     1021 agcaaattgg aatccctaca caattaaaag tcagttaggt gtaatcggac ttcagttata
     1081 tttccatgcc aatggaattg atcgtacgga tattgctatt ccaccagaac caaccaaaga
     1141 aaagtcttat gggaattcac aagttctacc aaaagattat acgaggagag aagaagtaga
     1201 actagtagtc aaggagatgg cagagcaagt agccattcga attagacaac ataattgcaa
     1261 aactggttgt gtacgcttag caattggtac gtcaattttg gaagaaaaat caggtttctc
     1321 ccaccagatg aaaataccaa ttacagataa tacgaaagaa ttacaaaatt attgtctttt
     1381 tttattcaat aagttttatg aggggcagga agtaagacat ataggagtaa cttatagcag
     1441 attagtatac acagattccc tacaattaga tttatttagt gatccaagaa aaaagattgc
     1501 tgaagaaaat ttggatagga ttattgataa gattagacaa aaatatggat ttacagctat
     1561 tatacatgct tcatctatgt tagaaggtgc aagaagtatt acgagaagca ctttaatagg
     1621 aggacacgca ggaggattag gaggtttgca taatgattga atataacgat agaggacgtt
     1681 taaaatggtc gggattttat ttgtctgatc atactgaaag aatcaatgca gacaatgtac
     1741 aacgtgctaa tcataatttg gcaaaagaac aaatgacgac tgaagagatt gcgaaaatac
     1801 tgagaactgc tgtcataaaa aatagacccg tctgcattca aaaagaggaa cgaaacgctg
     1861 aaggttacta tccagatgat gtagtaggaa aaattattgg atatgacgag cttggtgtat
     1921 ttgtggaaga agaaaaagtt cactatgatg aaattaggaa tgtttctttc gcggaattat
     1981 taaaatggag tcagatatga acattattaa aaaatacgaa ttcggttata ttacgtatga
     2041 acagctaata gaggaattat ggggatatgg tcaacaatta attaatgaag tggggattga
     2101 ttgcttttgt ttctatattg aatccggagc aggataccat aagaacagat actatatatt
     2161 tccatatcca ccaaaatagt ctcttagaga tttttaaatt ttctccaaat actttcaatt
     2221 attgtgaaat atttcccttt aacagaatta ggcagaagtc gcccatcctc tataggtagg
     2281 tgatgaatgc cgttcggtat gagggttacc gttggtaact cgaagaccga caactaaggc
     2341 gtattgcctt tttattttgt
//
LOCUS       DACECY010000151         2356 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.161, whole genome shotgun sequence.
ACCESSION   DACECY010000151 DACECY010000000
VERSION     DACECY010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.161"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..669)
                     /locus_tag="IWO38_002842"
     CDS             complement(<1..669)
                     /locus_tag="IWO38_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1062 family transposase"
                     /protein_id="HAQ0314850.1"
                     /translation="MTYTHLTSNKLAMIEAYYNNHQSVAKTAVLLNRSRQTIHKVYQF
                     FKTGHNALDYFNQYKKNKTRCGRRPIVLSDKQTEYIQKRVVQGWTPDVIVGRAEFSIS
                     CSMRTLYRMFKQGVFEVTHLPMKGKRKANGHKETRGKQSFRRSLRDRGNDYSKFNQEF
                     GHLEGDTIVGKKHKSAVITLVERLSKVIITLQPEGRRAIDIENRLNQWMQSVPKHLFK
                     SMTFR"
     gene            complement(751..2205)
                     /locus_tag="IWO38_002843"
                     /pseudo
     CDS             complement(751..2205)
                     /locus_tag="IWO38_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311502.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
BASE COUNT          765 a          485 c          370 g          736 t
ORIGIN      
        1 gcgaaaagtc attgatttga atagatgctt aggtactgat tgcatccatt ggttcaaacg
       61 attttcaata tctatagctc gtctgccttc tggctgaaga gtgatgatta ctttagataa
      121 tcgctcaacg agggtaataa cagcactttt gtgcttttta cccacaatag tatccccttc
      181 aaggtggcca aattcttgat tgaatttaga ataatcattt cctctgtcac gaagtgatcg
      241 acgaaaagat tgttttcccc gagtttcttt gtgtccgttg gctttacgtt ttcctttcat
      301 aggtaggtga gtcacttcaa atacgccttg tttaaacata cgataaagtg tacgcataga
      361 acaagaaata gaaaactctg cacggccaac aatcacatca ggtgtccaac cttgaacaac
      421 cctcttttga atatattctg tttgtttatc tgataagaca ataggacgtc tgccacagcg
      481 agttttattc ttcttgtatt gattgaaata atctagagca ttgtgccctg ttttgaaaaa
      541 ttggtaaact ttatgaatcg tttgtctaga tctattcaat agtactgcgg ttttggctac
      601 agattgatga ttattataat acgcctctat cattgcaagt ttatttgatg taagatgggt
      661 ataggtcatt tgtcttcact ccttataatt cttttgcggg aactatcttg agtgtagcat
      721 aaatgacttt tttatttgtc tagcttaatt ttacaatcgg cgattgtcac ttgcaatatt
      781 tactggaatt tcgctcgtat cttcttttac ttcctcagtc tcttcatcaa tacctaaaat
      841 gaatgaagca ataaaaccga caacaaatgc aatcgcacaa ccaatcgctg cgtaaataaa
      901 atttgatagt cctttttccc caaagaaact tggtaacgca agaatgccag gagcaacttg
      961 agcatatcga ccgacaccca tgattccaat aaatagtccc gctgctccac cacctaccat
     1021 tgaagcaatt aacggctttt tgaatcgcat attaattccg tataaagctg gttcagtaat
     1081 tcccataaaa gcagacagac ctgaagaaac agctaattgt ttgacagaaa gttgttttgt
     1141 tctaaaggca actgctaagg cagcaccacc ctgagcaata ttggaaacca tcatacctgg
     1201 accagcaaca gtatcaaatc cagatgttac taacatatta ataccgattg gaattaaacc
     1261 gtaatgcata cctgtcatca caagtaatgg agtaaatgca cccacaagta atggaaccaa
     1321 ccaacttgca tacgtattaa caaattcaat aagtgaccct aacccattac ctaagaatgt
     1381 acccaaagga ccaatcacaa ttaaggtaat tggtgcaaca atcaacaatg ttaacagagg
     1441 cgtcataaat attcgaacag cattgggcat caatttatcg ataatcggct cgatatatga
     1501 cataaatcaa atagctaaaa taatcggaat aacagaagaa ccatagttag ctaacgtcac
     1561 aggtaatccg aataaagcta aggactcacc tgtttctcta gcacccgcaa tcattgctgt
     1621 aaaagtcgga tgtaacaaaa ttgctcctaa ggtaattgca atgtactgat tacatccgaa
     1681 tttttttgca gcagaactag ctaataagac cggtaaaaaa taaaaggctg catcaccaat
     1741 gaaatttaaa aattgatagg tttgcgattc tggtgtcatc cattccaaca tgactaatag
     1801 agctaataat gcttttaaca tccctgcacc tgttaatgcc ggaacaattg gaacaaaaat
     1861 acccgcaatt gtatctaaaa cttttgatac aatcccttta ttgtttgctt ctgaactact
     1921 ttctttagaa gcggaaaaat ctcctagttt atcgatttcg gcaaatacaa attttacatc
     1981 tccaccaata atgacttgaa attgaccacc ctgattaact acacctacaa cacctttaag
     2041 atttctaata gctgattcat ctgctttact gtcatctttt aaattaaatc gaagtcgtgt
     2101 tgcacaatgc gctaaactgc ttacattttc agaaccacca acatttgcta atatgtcttt
     2161 tcctaattct ttatacccca tgctactttc ctccttagag aacattttct cttttgataa
     2221 tttgaatata gcgtgctatt aatagataaa caatacattt gaaaccgttt tacacagcgt
     2281 tgcgaaattc attcagttta cgattatttt ttcgcaacac tgttccaaaa aaataaaatc
     2341 cacttactaa ctaatc
//
LOCUS       DACECY010000152         2293 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.162, whole genome shotgun sequence.
ACCESSION   DACECY010000152 DACECY010000000
VERSION     DACECY010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.162"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(51..1598)
                     /locus_tag="IWO38_002844"
     CDS             complement(51..1598)
                     /locus_tag="IWO38_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ0314851.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IWO38_002845"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IWO38_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ0314852.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="IWO38_002846"
     CDS             complement(2043..2237)
                     /locus_tag="IWO38_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314853.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          644 a          477 c          408 g          764 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga aaacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agt
//
LOCUS       DACECY010000153         2292 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.163, whole genome shotgun sequence.
ACCESSION   DACECY010000153 DACECY010000000
VERSION     DACECY010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.163"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(22..837)
                     /gene="thiD"
                     /locus_tag="IWO38_002847"
     CDS             complement(22..837)
                     /gene="thiD"
                     /locus_tag="IWO38_002847"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ0314854.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            1131..1406
                     /locus_tag="IWO38_002848"
     CDS             1131..1406
                     /locus_tag="IWO38_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314855.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            1833..2288
                     /locus_tag="IWO38_002849"
     CDS             1833..2288
                     /locus_tag="IWO38_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0314856.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT          748 a          436 c          343 g          765 t
ORIGIN      
        1 ctttttcaca acttctttct tttactctaa ttcaccagct gcccaatgat tgatggggcc
       61 atatttgtgc cctacattta tttcttcacg aattgcacgg tttacatagt cttttgccaa
      121 tcggatcgct tgttcaactg tttttccttt cgctaactca gctgcaatac atgcagatag
      181 cgaatcgcca gttccgttga tccggtcggt aggataaaat ggtgcattta accaaaatga
      241 ttttcctgat tcgaacaata cgtagtcatt gactgtttct tgctgcgcat ttttagcatg
      301 atgtcctttg atcataacgt tttttgcacc catatcttgc agttcttttg ctgcttgcac
      361 aaaatcttct tcagctgcta atgtccgtcc agtcagcttt tctgcttcat aaaaattagg
      421 tgtcagtact tcggccaaag ggacaagctt ttctttcaag ctttgcaaag cagattcttc
      481 tagtaaaaga tttccatgtt tcgttacgat tacgggatcc acgactaatg gtccaaaatc
      541 atatttttgg taatttttta caactgtctc aatcaacttt gaatctgcta gcatacctgt
      601 tttagctgca cgtatttgaa agtccttcgc aatcagttca aattctttgt cgataaaatc
      661 tgttggcaat gtcacacttg ctccgattcc gtaagaatta cctgcaacac atgcggtcat
      721 gacagaaaca ccgtaaacat ttcttgtaaa gaatgtatgc atatctgctt gcatccctgc
      781 gcttccatcg ctatctgatc ctgcgatcgt cattgcttgt acatactgat cttccatcaa
      841 atcgattcct ccatttttat aaaaagattt ttcataatac tgttactgtt tctatcatat
      901 gtttttcaaa gtatccctca gtaaatatac cactaccttt tcttggtaat caaatttatt
      961 tgaaaaaaca tttgcaataa atcagaaaat agtggtcgtt tatttgtatt cgctattcta
     1021 ataataaata accactattt gatttatata tatttcagta tccttcttgt tttcatataa
     1081 ccaaaggcgc tatgatttcc atatgaaaaa aataaagtca ggtgaaaaaa atgaaacttg
     1141 aggataaaaa agtcattttg aagaaaatca aacgactacc agaccaaata gatggctata
     1201 cgatctgttc tttcaaaaaa gacaggagta taaaagctta tacgcaaaac acagaaaaag
     1261 gaataatcta tttcctacat gaagaaggtt acgataccca aacctttatg tttactgaaa
     1321 aaaaagaatt tcataaacag atgaaaaaac tgattgaaaa agaatttcca agaagtcata
     1381 aactctatgt gaatcagcaa ttttaacttc taactgttct ttattaatta aaaatgttct
     1441 gtaataaaat aaaactttat aacaactata acaaaaccga atgcaaaaca gaagttctat
     1501 aagttataat catagttgtt aggtgattca tctagtaaaa acaccagatt tcaaatttca
     1561 cacaatttaa actagatcaa tcatcaacct tcttcaaatt gttagaaccc tttcccgagg
     1621 agctgcagca taagcaaaaa aacaaacagg aagccaaatg gctaagacat tttttctatt
     1681 agtctagttg tttctcgtcg caagtttaag actttagaaa gttatttcta atgatgtttg
     1741 ggttgttttg atcaatgctt atgcacagct ccttacctat tatttatctc aacagatcta
     1801 acaaattaat ggactattta aggagttttt ttatgcttac gatctattct tctcgcacac
     1861 atagttacca tctgatggca aactggctga aagaccatca aatgccttac agagaacgaa
     1921 ttttcacacc taaacagcct ttaacaagga aagagatatt ttatttattg tcattaacag
     1981 aaaacggatt tgacgatcta ttagctaaac gctcgattga ttataaaaat ttgaaagacc
     2041 aaatcgacca atgtacgacc tcagagcttg tcgatctgat cgtctcgcat tttactttgt
     2101 tacaacgtcc catcattact gacggaaaga aattattagt cggattcaat gaagatagca
     2161 tgcgtctttt tatccctgta caatggcgca gaagtcaact gaaatctgtc tataacgatt
     2221 ttacatgggt aagcaatcta gaaaattcta cggatttaga tgatgaattt ttaaattatt
     2281 atatataaaa aa
//
LOCUS       DACECY010000154         2182 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.166, whole genome shotgun sequence.
ACCESSION   DACECY010000154 DACECY010000000
VERSION     DACECY010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.166"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            950..1423
                     /locus_tag="IWO38_002850"
     CDS             950..1423
                     /locus_tag="IWO38_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ0314857.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1432..1659
                     /locus_tag="IWO38_002851"
     CDS             1432..1659
                     /locus_tag="IWO38_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0314858.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          707 a          345 c          321 g          809 t
ORIGIN      
        1 tttttcggag aattaaaaaa tacaataaat aactttagtt actttaatgc gttcttactt
       61 tatttttata ttatcatttt tcttattcac ttctttggtt tatactattc ttataaataa
      121 aaacgaggta tgtcctatga tgaaaagatt gtactattca ttgatcataa cgattggcta
      181 tttgattgta tcaaatctag gaaatatggt atttggtatc agtaaagaat tcagttggac
      241 tactacatta tgggaatctc tattcttttt catcttcgta tttttgttac aaaattatcg
      301 aaaaaaataa acatgtgcca tctatctgtt ggcacatgtt ttaggattta tggtactttt
      361 tattcggcaa tccattttat ttttctactt cttcttaata cctctaacac ttttgattta
      421 taaactaatc caatgcatct acttgcgctt attaagctat ttcagctaga tagtaccacc
      481 ccatgcgtca atgcttgatt ttccttttgt ataacattgt ataagcaata aaggacaaat
      541 aagtggtgat tacaccaaac cattgaataa gaaaaattct gatacgtaaa taaacatgaa
      601 cttgaaaaaa ttaatgaatc ggttagctac tacgtagttg tcttttttct catacagttc
      661 tgttaactta cacttctctt cgctagataa taaaaaatcc tccaactgta ttgtccaata
      721 taatcccagg catctaaact agcattatag aagagacaca catagaatct aaaataactg
      781 gttttaaatg tttcttacaa tcaaaaaaag ctgttaaatg cactccacac cttcatactc
      841 tatcattttt tataaacgat atatttttat tgtttttcga taaaaaatag ttgttttaca
      901 ataattattc tgctatattt tagctatcaa agataaaaga gggataaaaa tgaaatttaa
      961 aacattgcta ttaaaatttg tcgttttagg tataagtgcc gtatttattt tgttcggcgt
     1021 acttttttat actcagatcg ctacaagcga aaaaggattc aattttgatt ggcagaccac
     1081 attatttgtt tctgtcattt actttgccct tctcctaggg atcgttattg cttatttctt
     1141 attgaaacta ttgaaaaaaa tcgaagataa ccaagctttt tccactcaaa gcttaaacat
     1201 actaaaaaaa atcgaacgaa ccattttgtt tattggtttc gccttttttg gcttacttcc
     1261 taaatttatg aaagtagcgg atttagacga tgctccaggt ttgatgttgg taggaatcgc
     1321 tgtcgtattc attccttttg cagtatacac attaacaagc gtgctggaaa aactgcttga
     1381 acatgcgatt ttacttaaga cagatcatga tttgacagtg tgaggtgcga catggcaatt
     1441 atcgttaatt tagatgtagt attagctcaa aaaaaaatga gtgtgactga attgagtgag
     1501 aaagtgggca ttacgatggc aaacatctct attctaaaaa atggaaaagc aaaagccatc
     1561 cgtttttcca ctctcgaaaa aatctgccaa gttttggagt gtcaaccagg tgatttgttg
     1621 acctataaaa atgactcgaa tactatacca gaagaataaa ataaagtaga acaaacagta
     1681 aataaaaaat aatctgaatg atagatgtgt gagactttta gaagttattt acgtctatta
     1741 ttcagatttt ttatttcgat tgaataccgt tttatctata aataagatat gtactgactc
     1801 atacaaaggg ggccttttct tctccatgta ctatcacttt tcctgataca actggtttcc
     1861 tgttacgttg tagttggatc tgcttttagt ataatacttt attcattcca ccagactgat
     1921 agcagtttga gcagtctaaa taatcatgta gtattacagg ccattctgtt tcataccttg
     1981 aagtgcttaa aacttcagct aatggcccat tgtgttaact cttactccat tcttttttag
     2041 taagctccca tatatactgt aaagccttct atatattatg tcatgttgct ttttaacatt
     2101 tactatgtag ttttgataaa atgatttgaa taacagtgat ggacaattct tttttatttt
     2161 taacattatt ttgataataa tg
//
LOCUS       DACECY010000155         2098 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.167, whole genome shotgun sequence.
ACCESSION   DACECY010000155 DACECY010000000
VERSION     DACECY010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.167"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..287
                     /locus_tag="IWO38_002852"
     CDS             <1..287
                     /locus_tag="IWO38_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAQ0314859.1"
                     /translation="KEKEAEKSIVGKCPKCGNNIVLKKSFYGCSNYPECKFTLAEHFR
                     KKKLTKTNVKELLEGKETLVKGIKNKEKKPYNAVVKIGEKGYIDFISFSK"
     gene            421..660
                     /locus_tag="IWO38_002853"
     CDS             421..660
                     /locus_tag="IWO38_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAQ0314860.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
     gene            709..792
                     /locus_tag="IWO38_002854"
     CDS             709..792
                     /locus_tag="IWO38_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ0314861.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            917..1654
                     /gene="erm(B)"
                     /locus_tag="IWO38_002855"
     CDS             917..1654
                     /gene="erm(B)"
                     /locus_tag="IWO38_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ0314862.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
     gene            1824..2084
                     /locus_tag="IWO38_002856"
                     /pseudo
     CDS             1824..2084
                     /locus_tag="IWO38_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
BASE COUNT          838 a          308 c          365 g          587 t
ORIGIN      
        1 agaaagaaaa agaagcagaa aaaagtattg taggaaaatg tcctaagtgt ggcaacaata
       61 ttgtattaaa aaaatcgttt tatggttgtt caaattatcc tgaatgtaag tttactttag
      121 ctgaacattt tagaaagaaa aaactaacca aaacgaatgt aaaagaatta ctggagggaa
      181 aagaaaccct ggtaaaagga atcaaaaaca aagagaaaaa gccctacaat gccgttgtaa
      241 aaattgggga aaagggatat attgatttta tatctttctc aaaataaaca taaaagccct
      301 ttaaagaggg cttttatata ttaatcacaa atcacttatc acaaatcact tatcacaaat
      361 cacaagtgat ttgtgattgt tgatgataaa ataagaataa gaagaaatag aaagaagtga
      421 gtgattgtgg gaaatttagg cgcacaaaaa gaaaaacgaa atgatacacc aatcagtgca
      481 aaaaaagata taatgggaga taagacggtt cgtgttcgtg ctgacttgca ccatatcata
      541 aaaatcgaaa cagcaaagaa tggcggaaac gtaaaagaag ttatggaaat aagacttaga
      601 agcaaactta agagtgtgtt gatagtgcat tatcttaaaa ttttgtataa taggaattga
      661 agttaaatta gatgctaaaa atttgtaatt aagaaggagg gattcgtcat gttggtattc
      721 caaatgcgta atgtagataa aacatctact gttttgaaac agactaaaaa cagtgattac
      781 gcagataaat aaatacgtta gattaattcc taccagtgac taatcttatg actttttaaa
      841 cagataacta aaattacaaa caaatcgttt aacttctgta tttatttaca gatgtaatca
      901 cttcaggagt gattacatga acaaaaatat aaaatattct caaaactttt taacgagtga
      961 aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga
     1021 aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac
     1081 gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaatac
     1141 tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta
     1201 taaaattgtt gggaatattc cttaccattt aagcacacaa attattaaaa aagtggtttt
     1261 tgaaagccgt gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt
     1321 ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct
     1381 taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact
     1441 tacccgccat accacagatg ttccagataa atattggaag ctatatacgt actttgtttc
     1501 aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat
     1561 gaaacacgcc aaagtaaaca atttaagtac cattacttat gagcaagtat tgtctatttt
     1621 taatagttat ctattattta acgggaggaa ataattctat gagtcgcttt tgtaaatttg
     1681 gaaagttaca cgttactaaa gggaatggag ataaattatt agatatacta ctgacagctt
     1741 ccaagaagct aaagaggtcc ctagcgccta cggggaattt gtatcgataa ggggtacaaa
     1801 ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc taagtgtggc aacaatattg
     1861 tattaaaaaa atcgttttat ggttgttcaa attatcctga atgtaagttt actttagctg
     1921 aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa agaattactg gagggaaaag
     1981 aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc ctacaatgcc gttgtaaaaa
     2041 ttggggaaaa gggatatatt gattttatct ctttttcaaa ataaacataa aagccctt
//
LOCUS       DACECY010000156         2034 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.168, whole genome shotgun sequence.
ACCESSION   DACECY010000156 DACECY010000000
VERSION     DACECY010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.168"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            34..864
                     /locus_tag="IWO38_002857"
     CDS             34..864
                     /locus_tag="IWO38_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ0314863.1"
                     /translation="MSLLIYTHVLSTFMSVIVIGFLFIGSLFSKRKEKKEMSDLILNM
                     IKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTNLQERALNISDSLIGAVNNDLVTFT
                     MGTIGVVSLILPLFFLKKFTNKEKIIYFCAVTTWFLSTNLFPWEILQKTPIQIIQFPW
                     RILGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAATEANYSHKIQSYK
                     GRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWRMHLIKHIIPK
                     "
     gene            complement(1858..>2034)
                     /locus_tag="IWO38_002858"
     CDS             complement(1858..>2034)
                     /locus_tag="IWO38_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ0314864.1"
                     /translation="KTVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRA
                     QRRFEGTLRYSLGY"
BASE COUNT          721 a          290 c          318 g          705 t
ORIGIN      
        1 agaaagaact gtgtgattcc tttagctatc agtatgagtt tgctaatcta tacccatgtt
       61 ctctccactt ttatgagtgt gattgttatt ggcttcttgt ttatcggctc tcttttctct
      121 aagcgaaaag agaaaaaaga aatgtcggat ctgatattaa atatgataaa agctgttggt
      181 tggaccattc ttttaacggc ttacgtttgg tatccgatgt tacaacaagt cctacatcaa
      241 aaaattaatc gtccttttcg cacaaatctt caagaaaggg cattgaatat ttctgatagt
      301 ttaatcggtg cagtaaataa tgatttagtt acgtttacaa tgggaacaat tggtgtggtt
      361 tcattgattc ttccactttt ctttttaaaa aaatttacca ataaagaaaa gattatttat
      421 ttttgtgcag tgaccacttg gtttttatca acgaatcttt ttccatggga aattttacaa
      481 aaaacaccta ttcaaatcat tcaatttccg tggagaatct tgggttttca agtgcttttt
      541 ggttcattaa tcttaaccat agtgttttta aagtggaaaa caagcaacaa aaaaagtatg
      601 tggagccttg taggaatagt tcttattatt tttactgtga ctgcagcaac agaagcaaat
      661 tattcgcata aaattcagtc gtataaaggt cgtttagtta tgaccaaaaa agatgttaca
      721 cgttatacat cctcaagaac tggaggccta tatgactatg caccattaga tgcattgaaa
      781 tataaagatc atttaaagaa acatgaagta aaagttggtg atcgatggag aatgcatctc
      841 ataaagcata taattccaaa atagtctata cagtagaaga tgccgatgag caaaatatca
      901 cattacctgt attcaattat tggggaacag ttgtgaaagt aaatgggaaa aagagcacta
      961 tagaaaatga caatggttta gtgtctatta aaggtacggg aaaaagaact atagttgagg
     1021 tttcatctac gtatccaact acgatgattc tatctttatt aattagttca ataactttta
     1081 tattcttatt tacacgttat ttaataagat gctgcggtta aatgaaaata gattagatag
     1141 atattataaa tatgttgatt ctattttcat agcaatggag aaaatacatt agcaatatat
     1201 aaaagaaggt atcaagagtc acagtgaatt tattccgaaa agtgaaaaga gcaaatataa
     1261 aatttaataa aaatatagaa ctaaatagga aaacgtagac gtaattttct acgttttcct
     1321 tttcatatat attaaatatt acggaaaatt atataaacta atttagtgtt ttgtttgtaa
     1381 ttcctaattt tgctccactt ttagataatt tttaggtcta tttaactcat cagacattct
     1441 atgctcattt ttctccaaat ttgtttcaac tctttcgtac ttaattttgc gtatttgaaa
     1501 attcttcttt tagagtactt ttattacgat actagtatga tatatatcta tagcaaacag
     1561 ttaaaatctt cctaaaataa gcgaatagca ttcctaaaac ctataaaatt tttagatgaa
     1621 gcgacaagtg gacaattaat aaactacttg aacatgaaat tatggatcac ctttcaaatt
     1681 tagaaaatgg aattcttata cttattgcgc aacatttatt gattgcaaaa ttatgtgaca
     1741 aaaagaacta gtagaagaag gaatgatggc ttgataataa agtactacct attaaaggta
     1801 gtactttatt taatttagta taaattaaca tactactacc cgtggtgcta gttaatttta
     1861 atatcctaaa gaatatctca aagttccttc aaaacgtcgt tgtgctcgac ttaatagaac
     1921 actgtaagcc agtaatatgc tttcaaatct actctctagc cctttaacag aacgtgagtt
     1981 gaaattttga caacctaatt tttctaagga agaaaaaaca gtctcaactg tttt
//
LOCUS       DACECY010000157         2020 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.169, whole genome shotgun sequence.
ACCESSION   DACECY010000157 DACECY010000000
VERSION     DACECY010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.169"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            100..570
                     /locus_tag="IWO38_002859"
     CDS             100..570
                     /locus_tag="IWO38_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ0314865.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="IWO38_002860"
     CDS             792..1895
                     /locus_tag="IWO38_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ0314866.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          686 a          328 c          460 g          546 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatact
//
LOCUS       DACECY010000158         1993 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.170, whole genome shotgun sequence.
ACCESSION   DACECY010000158 DACECY010000000
VERSION     DACECY010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.170"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(63..356)
                     /gene="rpmA"
                     /locus_tag="IWO38_002861"
     CDS             complement(63..356)
                     /gene="rpmA"
                     /locus_tag="IWO38_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ0314867.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(369..713)
                     /locus_tag="IWO38_002862"
     CDS             complement(369..713)
                     /locus_tag="IWO38_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ0314868.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(740..1048)
                     /gene="rplU"
                     /locus_tag="IWO38_002863"
     CDS             complement(740..1048)
                     /gene="rplU"
                     /locus_tag="IWO38_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ0314869.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            1330..1971
                     /locus_tag="IWO38_002864"
     CDS             1330..1971
                     /locus_tag="IWO38_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ0314870.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
BASE COUNT          601 a          436 c          351 g          605 t
ORIGIN      
        1 tttataaaca aaaaaggctg tgacaaaagt cttgtcacaa cctctttaat cataagattc
       61 aattaagctt cttgagctac tggataaaca gacacttgtt ttttgttacg gcctttacgt
      121 tcgaaacgta ctacgccatc aacttttgca aataaagtgt cgtctccacc gatacccacg
      181 tttacacctg gatagatttt tgtaccgcgt tgacggtaaa gaattgatcc acctgtaact
      241 gtttgtccat cagcacgttt agcacctaga cgttttgatt ctgagtcacg gccgttagat
      301 gtagaaccgc cacctttttt gtgggcaaat aattgtagat tcatagataa caacatggtc
      361 tgcacctcct atttatcatt aattgtttta atttggatat attctgagtt ttcagcttct
      421 atcgattgta atcctaataa aagattttct aatagaatct cagcaatatt gttttgttct
      481 tgtgtcattc ctgaagtgac ttcggtataa agataaccgc cttcttcttc attcatttca
      541 acaatcggct caaaaccggc taatgaggca atcccgttga cagtgctgat cgctaacgca
      601 gaaacacccg cacaaacgat atcacttcca taaggaccag catcagcatg cccggtcaaa
      661 gtgaatgaaa cgatctgacc tgcgtcattt cgcttaaatg ttcctttgat catatatagg
      721 ccttctttct caaacgctat taagcgttga tcgcgtcgat aaccactttt gtatatggtt
      781 gacgatgacc ttgtttacgg tgtgaatgtt ttttaggttt gtatttgaaa gtaacgactt
      841 tcttttgttt gccgtgtttt tcaacagtac cttcaacagt tgcacctgca acagttggag
      901 cgcctacttt tgtagattct ccgcctacta agataacttc gtcaaaaaca actttttctc
      961 ctgcttcaac gtcaagtttt tcaacataga ttgcttgtcc cacttcaact tttacttgtt
     1021 taccaccagt tttaataatt gcgtacatgc tataaagcac ctccttctga tatacttaga
     1081 ctcgccatcc gaagtgacag catatgctgt gcttattact tgttctgagc ggttgtagct
     1141 gtggagtcca caattacaac agtactagct taccagaaca gcaaccgtta gtcaactaaa
     1201 tttaaaatca aaaagtaaaa caaaaattca agcaatactt ctgcttttct atcctcacag
     1261 ttctaccaaa taaaatagtc gcattgtgtt tcttttattc ttataataaa agaaaaagga
     1321 ggagcataaa tggaataccc aaaagaactg atacaagaag cttctttaag aatgaacggc
     1381 cgtcctgtag aaggatttat cgctgggcaa ggacctcttc atcctaaact gatgctcgtg
     1441 ggagaagccc cagggaaaac agagattgat acgcatgttc cttttagcgg tcaagctgga
     1501 aaagaattga tgcaggcact ttcttcaact ggcttaacac gagaagaggt gtatatcacc
     1561 agtgctgtaa ggagccgccc gtatcgcgtg actcatcgga tcaataaacg gacacaacag
     1621 tcagaaactg tttatccaaa tcggacacct actcgttccg aagtatttgc gcatgcacca
     1681 atattggatt acgaactgat gaaggtccag ccaaaattaa ttgcgacact tggaaacatt
     1741 ggtcttcaac gtttacttgg caaagaatat acggttacca aaaatcatgg tcaactctat
     1801 acagggcctg tcttacaatt aacagaacaa aaggatgcct acgaatggtc agaaaaaaac
     1861 tatcgaattc ttccactttt tcatccagca gctattttct acaatcgctc ccttgtacct
     1921 gttatccatc aagactggca agtactagct tcacttttac agcaatatta aaaagaacag
     1981 acaagaccat aaa
//
LOCUS       DACECY010000159         1991 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.171, whole genome shotgun sequence.
ACCESSION   DACECY010000159 DACECY010000000
VERSION     DACECY010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.171"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            127..1851
                     /locus_tag="IWO38_002865"
     CDS             127..1851
                     /locus_tag="IWO38_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAQ0314871.1"
                     /translation="MKKKLLTLFLVMSFSIVLSVYYNTIVFSEQINYTEEQYNIEKEK
                     ALKVGYSEEQFKQIMEIPTNLSNENSETRIVNYTMTSNQTKVINKAMEQIGKPYEWGA
                     SGPNSFDCGGLVKYVYKQAVNIELPMGTTNQEQYGTEVSLNSLKPGDLLFYGNRGATY
                     HVGIYKGNGVMIHAPQPGETVKEVNIQYFYPNFAKRILPDEPDYPYIDYNKMVTVTKA
                     WSIWNDLQFSHEIKKAIIGDNYKIGKVYTNPENNNKYGEILVSNKVYGYINFYAVKEL
                     TSVQINRYLTSKDSGQPTWGNLECTISKGQTTKDKIYFVKGAYNLGDGKYLYSIYKDQ
                     DSSEWLGYLKAHVSLAYTPIEEINKNVTVTKNWSIWNNLQWEKEIEKPQIGSVFSARL
                     KLTNVSNNAVYYKLYKSGKFYGYINAEAVKDLTTTKLNKYVTFSVNNEDFWSSLDVNY
                     SKGKTERGRVFYISISYNTADNQPIYSVYTDETCTEWRGYYKGNNFEDTQITMLENKS
                     VKVTKSGYTVWGDLNFWTKSGISNTGDIYTTDRKFYNFTNNAYYYELKKDGRVYGYIN
                     SEAAVEMN"
BASE COUNT          780 a          241 c          348 g          622 t
ORIGIN      
        1 agtttgtaac aaacatatat tttttatatt acaatatttc aacaaattaa aaaaaattat
       61 atcttcttaa acaatgataa tattaaaatg caagcgcttg taaaaaataa ggagaagagg
      121 taaaaaatga agaaaaaact attaacttta tttcttgtga tgtctttctc aatcgttttg
      181 agtgtgtact acaatactat tgttttttca gaacaaataa actacacaga agaacagtat
      241 aatatagaaa aagaaaaagc tttaaaagtt ggttatagtg aagaacagtt taagcagatt
      301 atggaaatac ctacaaattt atcaaatgaa aatagtgaaa caagaatagt aaattatact
      361 atgacttcta atcaaaccaa agtcattaat aaagcaatgg agcaaattgg aaagccatac
      421 gaatggggag catcgggacc aaattctttt gactgtggtg gtttagtgaa atatgtttac
      481 aaacaagctg taaatattga actgcctatg ggaaccacca atcaagagca atacggtaca
      541 gaggtaagtt taaattcact taagcctggt gatttattat tttacggcaa tcgaggtgca
      601 acttatcatg taggtatata taaaggtaac ggtgtaatga ttcatgcacc gcaacctgga
      661 gaaactgtaa aagaggttaa catacaatat ttttatccta actttgcaaa aagaattttg
      721 cctgatgaac ctgactaccc ttatattgac tataataaaa tggtaactgt tacaaaagct
      781 tggtcaatat ggaatgattt acaatttagt catgaaatta agaaagctat tattggagat
      841 aattacaaga tagggaaggt ttatactaat ccagaaaata ataataaata tggagaaata
      901 ttagtaagta ataaagtata tggatacatt aatttttatg ctgttaaaga gttgacctct
      961 gttcaaataa acagatattt aacttctaaa gatagtggtc aaccaacttg gggaaatcta
     1021 gagtgtacta taagtaaagg acaaactaca aaagataaaa tatattttgt taaaggagca
     1081 tataatttag gggatggaaa gtatctatat tctatttata aagatcagga ttcctctgaa
     1141 tggttagggt atttaaaagc acatgttagt ttagcctata ctcctattga agaaattaat
     1201 aaaaacgtaa ctgttactaa aaattggtcg atttggaata atctacaatg ggaaaaggaa
     1261 attgaaaagc ctcagattgg ttctgtattt tcagctagat taaagttaac caatgtatcg
     1321 aataatgctg tctattacaa gctttataaa tctggaaaat tttatggtta tatcaatgct
     1381 gaggctgtca aagaccttac aacaacaaag cttaataagt atgttacatt tagtgttaat
     1441 aatgaagatt tttggtcttc tttagatgta aattattcaa aaggaaaaac tgaaagaggc
     1501 cgagtatttt acattagtat ttcatataat accgcagata accaaccgat ttattcagta
     1561 tatacagatg aaacatgtac ggaatggcga gggtattata agggaaacaa ctttgaagac
     1621 actcaaatta ctatgcttga gaataaaagc gtgaaagtaa caaaaagtgg gtatacagta
     1681 tggggagact tgaatttttg gactaagtca ggaatatcga acacaggaga tatatacacc
     1741 actgaccgaa aattttataa ttttaccaac aatgcttact attatgaatt gaaaaaagat
     1801 gggagggtct atggctatat aaatagtgaa gcagctgtag agatgaacta aaatattgct
     1861 gtcccttaat cttatggggc agcaatattt ttatattatt aatcattttt ctgaagcgag
     1921 ggtagaagaa ttaatcaata gtttttcttg ttttgtaata taatagttta attaccaaaa
     1981 attatttaag t
//
LOCUS       DACECY010000160         1910 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.173, whole genome shotgun sequence.
ACCESSION   DACECY010000160 DACECY010000000
VERSION     DACECY010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.173"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            492..710
                     /locus_tag="IWO38_002866"
                     /pseudo
     CDS             492..710
                     /locus_tag="IWO38_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            970..1224
                     /locus_tag="IWO38_002867"
     CDS             970..1224
                     /locus_tag="IWO38_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0314872.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            1225..1542
                     /locus_tag="IWO38_002868"
     CDS             1225..1542
                     /locus_tag="IWO38_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0314873.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          661 a          300 c          355 g          594 t
ORIGIN      
        1 cattatatca ccaaacccca tcagcgtgca ggctgatggg gtttttttac gtttctatat
       61 tatagaatta acgggtaggc ttactgtctt ttttggcaat tttgcaatca agccatttgc
      121 agaggtaact gcacactaca tctgcaaatg gcttgattgc aaaaacaaaa tcaaaaaggt
      181 atttcattat atctcctcct ccccttgaca gattaggccg gggtagcaga cagcaaaact
      241 attataacat aactgaaata cctaaatgac atatacagat atgataattt atgtattgat
      301 ttttctaaca aaagcacata ttttaatata tactacctat tttaggtagt atatattttt
      361 agatacaata tcgatcagtt ttgaggaaat gaaattctag atattttttg ctaaatagag
      421 ttactttcaa tattttttaa cgtaattagg cagaagtcac tcatcctcta taggtttgtg
      481 atgaaattgc cattgatcca tttaagtaag agcaaaaaac tggaggttta tcaggtaatt
      541 ggtctaatgg atttaagtat gcggtcaccg tgtatagagt tgcttatgag attcaagaaa
      601 ataaagttat tccagtattg ctatatggaa cacataacaa tttttatgaa ttagccgaga
      661 acactcgcaa ctttattcgt gtagatgaat cggcttcggt tgagttataa ctttagctat
      721 atcaatcaac cgaccgacat atgttaaatc tatatataat aaaaatacct acttaaatgg
      781 atgttaacgg aagtgtatgg tgagtagttc actcgtattt tgatatcgtt tgtatatgtt
      841 ttcagcgttc taagtggctt attttgtcca ataataatat ggagtggaat cccttaagaa
      901 acaccgttgg caattgttgt tataacatgt tataatttat tcatcaaaaa ctaaaaggag
      961 tgtgataaca tgggaaaaga actaaaggtt agaaagatcg gcaatagcgt gggagtgatc
     1021 cttccaagct ctcttggatt aaaaagcgga gacactatcc aagccaaaca agaaggcaac
     1081 ttattcatat tagatactac tcaaatagca aaagaacacg atcgcaaatt gattgaagaa
     1141 agtttccaag actttgagaa aggtttaaca gtttcagaaa ttgaaatggt aaaagctttc
     1201 ggaaaatacg gctggagtga atagatgaaa gagtacacgc tagtttattc taaaagtttc
     1261 cagaccagcc ttgaaacgat aatagaagaa tgggaaaatg agcttgctct gtctccagcc
     1321 agcataaaaa gatttgtagg tgcgatccag aaggctttag aactaaccaa aaattttcca
     1381 actatgcatg aggaagtttc cgaaatctat ggaatggata tcccaacata tcggatcgtg
     1441 atcggcaaaa gctatgcctt attttatcgg atcgaccatg aaaaaaaaga gatacttgta
     1501 ggacgtattt tccaatctaa acaaatgaaa ctaggctttt aacaataatt aggcaaaagt
     1561 cgctcatcct ctacaggtgg gtgattaatg ccgctcgata tggttgttaa ccgtcggtta
     1621 ctcgaagatt gatatataag acttagtacc tttttatttt gctccatgtt atggccgaag
     1681 gtctaaagat acaatcatta gtctagagaa aaattaagaa ccgtaggaac tacggggatc
     1741 gcttggtgaa taagagacac tgctattagt gaaaaaataa gatatcaagt acgctctatt
     1801 cccaaaaatt tcgtgacttt actcatgaga ggttcaagta gtatattttt aatcaaaatt
     1861 gacgtaactt ttaataaata ctacctaaat aaggtagtat ttatttttgt
//
LOCUS       DACECY010000161         1888 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.174, whole genome shotgun sequence.
ACCESSION   DACECY010000161 DACECY010000000
VERSION     DACECY010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.174"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            6..398
                     /locus_tag="IWO38_002869"
     CDS             6..398
                     /locus_tag="IWO38_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0314874.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            399..1838
                     /locus_tag="IWO38_002870"
                     /note="aph(2'')-Ia"
     CDS             399..1838
                     /locus_tag="IWO38_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAQ0314875.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          787 a          138 c          322 g          641 t
ORIGIN      
        1 agtttatgga agaagtttta attgatgata atatggtttt tgatattgat aatttaaaag
       61 gatttcttaa tgataccagt tcatttgggt ttatagctaa agaaaataat aaaattatag
      121 gatttgcata ttgctataca cttttaagac ctgatggaaa aacaatgttt tatttacact
      181 caataggaat gttacctaac tatcaagaca aaggttatgg ttcaaaatta ttatctttta
      241 ttaaggaata ttctaaagag attggttgtt ctgaaatgtt tttaataact gataaaggta
      301 atcctagagc ttgccatgta tatgaaaaat taggtggtaa aaatgattat aaagatgaaa
      361 tagtatatgt atatgattat gaaaaaggtg ataaataaat gaatatagtt gaaaatgaaa
      421 tatgtataag aactttaata gatgatgatt ttcctttgat gttaaaatgg ttaactgatg
      481 aaagagtatt agaattttat ggtggtagag ataaaaaata tacattagaa tcattaaaaa
      541 aacattatac agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg
      601 ttcctattgg atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt
      661 atccaaaaac tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt
      721 ggagtaaagg aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa
      781 gaaatgctaa tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat
      841 accaaaaatc tggttttaga attattgaag atttgccaga acatgaatta cacgagggca
      901 aaaaagaaga ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg
      961 caatgaaata tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa
     1021 tcggtagtgg ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa
     1081 aatttagtac taataagaaa aaaggttatg caaaagaaaa agcaatatat aattttttaa
     1141 atacaaattt agaaactaat gtaaaaattc ctaatattga atattcgtat attagtgatg
     1201 aattatctat actaggttat aaagaaatta aaggaacttt tttaacacca gaaatttatt
     1261 ctactatgtc agaagaagaa caaaatttgt taaaacgaga tattgccagt tttttaagac
     1321 aaatgcacgg tttagattat acagatatta gtgaatgtac tattgataat aaacaaaatg
     1381 tattagaaga gtatatattg ttgcgtgaaa ctatttataa tgatttaact gatatagaaa
     1441 aagattatat agaaagtttt atggaaagac taaatgcaac aacagttttt gagggtaaaa
     1501 agtgtttatg ccataatgat tttagttgta atcatctatt gttagatggc aataatagat
     1561 taactggaat aattgatttt ggagattctg gaattataga tgaatattgt gattttatat
     1621 acttacttga agatagtgaa gaagaaatag gaacaaattt tggagaagat atattaagaa
     1681 tgtatggaaa tatagatatt gagaaagcaa aagaatatca agatatagtt gaagaatatt
     1741 atcctattga aactattgtt tatggaatta aaaatattaa acaggaattt atcgaaaatg
     1801 gtagaaaaga aatttataaa aggacttata aagattgatt atataatata tgaaaagcta
     1861 ttataaaaga cattagtatt aaatagtt
//
LOCUS       DACECY010000162         1770 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.175, whole genome shotgun sequence.
ACCESSION   DACECY010000162 DACECY010000000
VERSION     DACECY010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.175"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            197..424
                     /locus_tag="IWO38_002871"
     CDS             197..424
                     /locus_tag="IWO38_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ0314876.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            547..873
                     /locus_tag="IWO38_002872"
     CDS             547..873
                     /locus_tag="IWO38_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ0314877.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            941..1654
                     /locus_tag="IWO38_002873"
     CDS             941..1654
                     /locus_tag="IWO38_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ0314878.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
BASE COUNT          686 a          273 c          373 g          438 t
ORIGIN      
        1 aacaaagtag ttgacacttc cgctacttag tattagtacg aaagtatact tcatgatact
       61 aagtagataa gaagtgaaaa gcggaaccca cgatcgtcaa aatgctaact gctttgctta
      121 caccagataa agggaagagc aatatcgctt attttaggcg tggagaagca agcaaatccc
      181 tacgatcaca aaaaaaatgc tgacccagca gttaagagag ttagaacaag atgaaagtat
      241 ccatcgaaaa gcctataacc aagtcccgcc taaagtagag tactcgctca ccgaagaagg
      301 aaaatcatta agagagattc tagtagcgtt gtctgtttgg gaagaagaac gtatcgaaaa
      361 acaaaaagcc tcaagggaag aagacacgct actgcataca catggtggct acctaaatta
      421 ttgattttct atgacattta ttttaaataa aagaaaacag gagttttttt aaaagcatca
      481 tctatttttt atgtaaaaaa atggtatgat taatcaagta agaagtgatt ggaagaaggc
      541 aagcaaatga atagtatgga aaaagtcaac cagtttagag atgaacgtaa ttggcgacaa
      601 tttcataacg aaaaagattt ggctatctct atttctttgg aagcatctga attgttagaa
      661 ctttttcaat ggaaaacgcc ggaagaagta aaagaaacac aattagaacg aatcaaagaa
      721 gaactggcag atgtattgat ctattcatac atgatggcag acaaccttca cctggatcta
      781 gatgagatca ttgaagaaaa attgatcaaa aataatttga aatatcctgt agaaaaaagc
      841 gcaggtgtac gaaaaaaata cacagagttg tagaaatgtg tcattcggct gttggaaaga
      901 gcaaaagaaa aacagtaaaa tgagtctgga gggagagaaa atgaaaaaaa aaggaaaagc
      961 aaaaagatgg ctagtcaata tcctcttgtt cctcttgtta ttagtaggac tagctttgat
     1021 tttcaatgaa cagatcaagg attattttgt cagagagaca ggagataaat atgctatagc
     1081 gaatgtaaca aaagaagatc tgaaaaagaa taacgataaa gatgtcagtt ttgactttga
     1141 tgcggtagaa ccaatgacga cagaaggggt catgaggtcg caaatgagag gaacagatct
     1201 accagtgatt gctagtatcg cagtcccttc agtttcaatc aatttaccgg ttttcaaagg
     1261 cttggataat acgtcactac tttacggagc aggaacatta tctccagatc aagaaatggg
     1321 aaaaggaaat tatgccttag caagccatcg tgcaacgaac cctgaattgc tgtttacacc
     1381 gctagaaaat ttagagatgg gtgccaagat ctatttgaca gatttagaaa atgtgtatac
     1441 atacaaaaca ttttttaaag aaaaagttgc tcctacagat acccaattac tgaatgaagt
     1501 cgagggaaaa gaaattgtca cactaattac ttgtggcgac atggacgccg tcacgcgact
     1561 agttgtacaa ggagaactgg aaagtgttac atcgataaaa gatgcgacag atgatatgag
     1621 atctgctttc aatctagaga caaaaacatt ttaggatact gggacataag tcgttacaga
     1681 ttaaaaacag ccaaaggata tgaatcgata aggaaaaacc aatttgctga aaaaaagaga
     1741 aacgcaaaga ggtgcatctc tgatttggtt
//
LOCUS       DACECY010000163         1637 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.177, whole genome shotgun sequence.
ACCESSION   DACECY010000163 DACECY010000000
VERSION     DACECY010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.177"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(183..1502)
                     /locus_tag="IWO38_002874"
     CDS             complement(183..1502)
                     /locus_tag="IWO38_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAQ0314879.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          485 a          301 c          325 g          526 t
ORIGIN      
        1 attctttggc acattttatt gaaatatgta tcactaatat ccctatcaac tgattttgac
       61 taaaaagtta cttcaaacat tcactgttca aaaatattga ggaataaatt ttgggtgtaa
      121 ggcattttga gcatacttct ccctggataa cttcccagta aaaaaatcgt tagattgttg
      181 gattagatcc attgactggc tcgcctcagg cgagcaaaga ccctgtagaa ttcattctga
      241 tacacatgat aactagataa tttgagatag actcgtctac ccgtttggac taatttccca
      301 gcaactttca gaaacttcaa tcgaatagaa tgaatggtca ttcccttttg gacttcttca
      361 aagccaatcg ttcttaagaa gttgactaag ttgtaagcta tcaagctgag catcattcgg
      421 acatgattct ccaaaaagcg aggactgtct gttttgtcaa agtaaaagcc agctttcgct
      481 tctttaatga agttctccat tgtgccacgt ttggcataga tagaaaagat ggtatcagga
      541 gaaacatttt ctgatagatt cgtcacgata aattcatgtc gaaagagtag ttcgcccgct
      601 tcacgtgttg agcgtataca cacgcgacga ctttgtgacc aggattgtgc ttgataagtg
      661 gtcgagaagt actgaacttc tcgttcttcc cacttttgat tatcgccata aagtactgat
      721 ttctcagcta tttgacctag tctacgatta ttcttcagtc gaataacata atgactcttt
      781 tttgattcac acgaatcata cacaccaggt gtagcgaacc cgctgtctcc acgaaccaag
      841 atgtcagtgt ttggtagaga gtgattatag tgctctaata aaggtgtgag aaactccttt
      901 acgccttttg atgtatattg atttcctgaa cgtagttcag cttttaagaa gtcaccagtc
      961 aatccgtcaa aagctaccaa tggatggtaa ccataggttt ggtagtgtgt attataattc
     1021 gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc
     1081 gtatcattac ggatgaggcg tgctttatcg ataagttctt ggttcaaagc ttgaagttca
     1141 tggatattct cctcagatag tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt
     1201 tctttcctat ctaaaacagc tttaaacaca ggatcttgtc tcaggagatt agctgcagaa
     1261 tcagctgagt aaccagcaat taattgcata atgaactgct caagtatgga taagttgtca
     1321 tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt gcttagccag ttcagaaaaa
     1381 ccaaagatgt tcatcagctc tttcactagg acgagacccg aatcactcga taattgacca
     1441 cctgtatgag aaatagtgat atttgaattg aattttactt ggtttttgtg taagctagtc
     1501 atgaaaagaa ctcctttctt ttggttggtt tagtcacttt aaccatagca gaaaggggtt
     1561 cttttttcat cacttaacgg gtgaagatga aaaagtaatt gtaagctgcc gtgaggcagt
     1621 ttcacatggt tttaatt
//
LOCUS       DACECY010000164         1534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.178, whole genome shotgun sequence.
ACCESSION   DACECY010000164 DACECY010000000
VERSION     DACECY010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.178"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            96..506
                     /locus_tag="IWO38_002875"
     CDS             96..506
                     /locus_tag="IWO38_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0314880.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     TLAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            520..876
                     /locus_tag="IWO38_002876"
     CDS             520..876
                     /locus_tag="IWO38_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0314881.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVEKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          564 a          227 c          281 g          462 t
ORIGIN      
        1 ttattttgat ttttttttat attttattag ctaataattg gtattttaaa agaaattact
       61 gtagaattgg attataatta aaaaggagtg agaaaatgat aaaagtatat acttcgccta
      121 gctgtacttc ctgtagaaaa gcaagagcat ggttgcaagc gaatcaactt gaatttgaag
      181 agaagaatat ttttgccgaa ccgttaacag aaagtgagat caaaaagacc ttggcagcga
      241 ctgaaggagg agttcaagat attatttcca ctcggtcgaa aatttacgaa aagctgggca
      301 tagattttaa cgaattaact ttaaagcaaa tgatcgtctt attcaaagaa tatccttcat
      361 tattgagacg acctattttg attgatgaaa aaagaatact aatcggattt aatgaagatg
      421 agatccggtc atttgtccct cgtgaaattc gatgtgttgc cagaaatctg atctatttac
      481 aaaacaataa catcttaaat tcatagggag ggagccttcg tggaagtgaa agtctatgga
      541 tcagcgcact gtgtaacaag tctgaaggcg aaacaatggt tagaacacca tcaattgcct
      601 tatcaatttg ttgacttaga agaacgcgat ctaagtacag aagaagcaga agaattatgc
      661 agttttgaag aagtgcaagc agaacagcta tttgcgactt ggtctgaggc ttttcacaac
      721 attgcgattg acctttcctg tgtagaaaaa aaacaactga tttttttatg cacacaacat
      781 accaacctcc tcagacgtcc tttgattatt atcgatgaag ttctatttat tggatacaat
      841 gagcagttga tggaacagct tatcttaaat aaatagactt taactgtttt aatcaagtca
      901 ctgagcattc catacagttg taacagtcga acacttagca tcaagcgtgt tatgtgttct
      961 ttttttgact ttatttttct actatagaga catgctcaat ttatgtggtc aaaattcaaa
     1021 tctaggtggg gatatagagt taaaaatata ttgacatgtg taaatttaca tgatatattc
     1081 atttctgttg ttttaagtag caaagcaata aaatagtatt ttaaaaaaca tataaaatag
     1141 ttgttgacat gttagaaatg acatgataag ataactgagt cgttaaaaaa ggaccgcttg
     1201 cgaagcttga ttctatcgaa aaaacttcaa aaaagttctt gacaaataac aaacgacaag
     1261 ttatgataaa tgagttgctg taagatttcg aaaaacgaaa tcaaaaaaac ttttaaaaaa
     1321 gagttgacaa ttacttgtct ctctgatatg atataaaagt tgctgcggca gccaaaaacg
     1381 aatcgggaaa cactcctgat gaagtagacc tttgaaaaat gaacaaagta agacaaacca
     1441 aatgtgtagg gcgtcttgat tcaattcaag acaacaaaca tttttaacaa gcaagcaata
     1501 tgctagcaaa caaattgagc ttaacaatcg caag
//
LOCUS       DACECY010000165         1496 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.179, whole genome shotgun sequence.
ACCESSION   DACECY010000165 DACECY010000000
VERSION     DACECY010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.179"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1..307)
                     /locus_tag="IWO38_002877"
                     /pseudo
     CDS             complement(1..307)
                     /locus_tag="IWO38_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(561..1115)
                     /locus_tag="IWO38_002878"
     CDS             complement(561..1115)
                     /locus_tag="IWO38_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0314882.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          485 a          256 c          155 g          600 t
ORIGIN      
        1 aaacctatat aatcatgaat gctggcaaaa atttttttaa gtcaagttct aaatcttctt
       61 cgaaaacaca tttaatttct cctactgata attgatactc tatattacat gacttaatta
      121 gttttttaga tcccccctcc agataggcat atgtttcaat aattatgttc tttttattgc
      181 ttggcaaatt tccgttattt attgcataaa ttaaaaatga ctgtctattt agcccataat
      241 ctaatacaac ttcttcatac tcatatttct ttaaatattt aatatgaaaa tatgctgaat
      301 taatcaaaac tcgagtgtta ctgttgttat ttattgttgt tctattgtgt ataaaattgt
      361 ataggttgtc atattttctt ggtttattaa aatcattctc tatataataa tcattatcaa
      421 ctacaacatt ttgttctatc tctggtattc tattgtaaat gttactaaac gttttaaata
      481 cttccgaaaa ttcagttatc ttcattaatc tcaccttcta agttttcatt atacacaaaa
      541 aatatatcct aatatctcaa ttagcctccc aatttaaaac cacgtaacga tgcccctatt
      601 tcatcttcag ttataccaat gtaccttttt gtaattgctt gcgagctatg attaaaaata
      661 aacattaagg tagcaatgtc tctagttttc ttgtagtagt gatacccaaa agtttttctt
      721 aatgtatgtg ttcctatatc atctcttcct aatttttctg ctatatctgc atatatctga
      781 tagacacgat cccttgttat atgtcgacct tgttgccgac ttggaaataa ccaatcattt
      841 tcttctttac cttcagtata taataaaatc tcttctctca aattatctat aaaaatcggt
      901 tttcgcttac ctgttttatg ttcaacaata ataggcttac ttttacctcg tatgtctcca
      961 acttttaatc ctataatatc tgacatacgt aacccattat tgattccaaa caaaaataaa
     1021 aaactatctc tttctggatt acttgttctt cttagccaaa actgaaaatc ttgtatctct
     1081 tcatttgtcc ttaacggttg tacattatag ctcactattg tttcccttct taaaaatatt
     1141 ctgagttcat gattatatat atattttacc ataatcatga acttatatat atactctgtt
     1201 aaaagtgaag aaaatcaagg ggtttagttt atgattttat atcatttcag taactcagaa
     1261 tattccgact tttttgtaca aaaaaattaa ccttacttat atgaagattc taagctctta
     1321 tttaaatttt agacgggcag ttatccgtag aagttcttag aatagctaat ttacataatt
     1381 aaattatatg aaatttaaat ttgaacaata tactaagaac tccccttact ataaattgaa
     1441 ataaattagc atatgaataa ccgtgctacc aagcttttca ttaataaaat attttt
//
LOCUS       DACECY010000166         1470 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.180, whole genome shotgun sequence.
ACCESSION   DACECY010000166 DACECY010000000
VERSION     DACECY010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.180"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            65..>1470
                     /locus_tag="IWO38_002879"
     CDS             65..>1470
                     /locus_tag="IWO38_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa12 family transposase"
                     /protein_id="HAQ0314883.1"
                     /translation="MMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFDFIYPIV
                     ESTYSTHGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYSFEDPIP
                     HFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANANKHKFT
                     KEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESGYYVKGE
                     REKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRVVADAGY
                     KTPKFVHFLSHLKLYPILPYTVPHGVKGMFRKKDFVYDAYFDCYLCPNNQPLLFSTIT
                     RDGYRTYKSNPKFCRSCPLLKNCTLSTKHQKIVTRHVWADLMDELEHQRHTALNREIY
                     KKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLWKAKKPM
                     AFYLKFLQRLTKRSWQLS"
BASE COUNT          500 a          250 c          265 g          455 t
ORIGIN      
        1 ggagtgttgt ttgcagtttt tttgttttat actcattgta taaactattc tgggagtgat
       61 tctaatgatg agaaaacaat cgattgaagg tagaaatcaa tttgctatgt taacaattga
      121 tgatcttgta cctaaagatc acttagttcg aaagattgat gctgcgattc agtttgattt
      181 catttatccc attgtagaat ctacgtattc gacccatggt cgtcccagta ttgatccagt
      241 ggtcttaatt aaattagtat ttatccaata tttatttggt atccgttcga tgagacagac
      301 tattaaagac gttgaaacaa atgttgctta tcgttggttt ttaggttatt cattcgagga
      361 tcctatcccg catttctcta cttttggtaa aaactatgtt cgaagattca gagaaacaac
      421 tttatttgaa gacattttct ctcatattct agaacaagct gtaaaagctg gcttcgttac
      481 ggaagacaac ctttatattg attctactca tattaaggca aatgccaata aacataagtt
      541 cacaaaagaa atgacctacg gtcaagctaa agcttatcaa gatgaattag aagatgaaat
      601 aaataaagaa cgtattgctg taggaaaacg tccttttaca tgggacacag aaagtgagtt
      661 gagtcttcaa aaaataagtc attcagatcc agaaagtggt tactatgtta aaggtgaacg
      721 agaaaaacaa tttgcttatt ccgcgcatac ttcctgtgaa gataatggtt ttatcttatc
      781 tacattaatc acaccaggaa atgtacatga cagccaagtc gcaatcgatc tggtcaaaca
      841 gtcgaaaaga gcttttccaa atataaagcg tgttgtcgct gatgcaggat ataagacgcc
      901 taaatttgtt cattttctgt cacacttgaa actctacccg attcttccat atactgtacc
      961 tcatggagta aaaggaatgt ttcgtaaaaa agactttgtt tatgatgcct attttgactg
     1021 ctatttatgt cccaataatc aaccgttgtt attttcaact atcactcgtg atggttatcg
     1081 aacctataaa tcaaatccaa aattctgtag gagttgtcca ttacttaaaa actgtacttt
     1141 gagtacaaaa catcaaaaaa tagtaactcg acatgtttgg gcggatctta tggatgaact
     1201 tgaacaccaa cgccatacgg cgttgaatag agaaatttat aaaaagagaa aacaaaccat
     1261 tgaacgaata tttgcagatg caaaagaaaa gcatggtatg cgttggacga aatatcgagg
     1321 tctcgaaaaa gtcgccacgc acacgatgct tacgtttgct gccatgaatt taaaaaaact
     1381 agccacatgg ctatggaaag caaaaaagcc tatggctttt tatttaaaat ttctacaaag
     1441 actaacaaaa aggagttggc aattaagcca
//
LOCUS       DACECY010000167         1455 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.181, whole genome shotgun sequence.
ACCESSION   DACECY010000167 DACECY010000000
VERSION     DACECY010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.181"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            108..1040
                     /locus_tag="IWO38_002880"
     CDS             108..1040
                     /locus_tag="IWO38_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ0314884.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1455
                     /locus_tag="IWO38_002881"
     CDS             1112..>1455
                     /locus_tag="IWO38_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314885.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQ"
BASE COUNT          640 a          169 c          227 g          419 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caaga
//
LOCUS       DACECY010000168         1424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.182, whole genome shotgun sequence.
ACCESSION   DACECY010000168 DACECY010000000
VERSION     DACECY010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.182"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(150..1337)
                     /locus_tag="IWO38_002882"
     CDS             complement(150..1337)
                     /locus_tag="IWO38_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ0314886.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACECY010000169         1383 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.183, whole genome shotgun sequence.
ACCESSION   DACECY010000169 DACECY010000000
VERSION     DACECY010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.183"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(27..1046)
                     /locus_tag="IWO38_002883"
     CDS             complement(27..1046)
                     /locus_tag="IWO38_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0314887.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1057..1263)
                     /locus_tag="IWO38_002884"
     CDS             complement(1057..1263)
                     /locus_tag="IWO38_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ0314888.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          380 a          297 c          231 g          475 t
ORIGIN      
        1 taatgctcct caacgaggag ctatttttat cgtttaggaa tatttaaata ccaacgtttg
       61 tcatggaaat cttgcgcacc gcctttagtg tttccctctg gatcattcgt tgcccgcatc
      121 atgacataga ctttcttatt agggaagtta cgcatgttaa aagatacatg atagccaaca
      181 tttccagaag tattataagc ttgatttaca tctgatctat aaattccatc agctcttact
      241 cgagctaatt ctttcccagt attgtaatcc ataatgaaga tatactcgta tttatagtta
      301 gcaatgtgcc atccagctac atgcaaattt gcattttcga tttctccaaa ctgatcaatg
      361 tgggcgtaat tcgttccatc tgtcagtgta ggattagctg caccagctcg tgttggatca
      421 atgacaggct tgttttcaga agttgttgga ttttcatcgg taaatccatg agctaaatca
      481 tatgctaatt tttctttact tacgcccatt tcagaaagat aaccgtaagg atctgtatga
      541 tcgccccaga tgttttgtgt tacccataaa tgcgatttga ttcccggttg gttataagga
      601 gtgtccaacg ttaatggaat accatatttc attgctgaat ctctagccaa ttcaacgtat
      661 gccttgtagt ttttctcaaa cgttgcttta tcatgtgtgt gttgtaactc aatctgcaca
      721 ggactgttgg cattagcata cgaaccagca ccgtactgta cataaccagg ttgaccgact
      781 tgataaacaa ttccgccgtc tcccacaata taagcagtat aagcgctagt ccatgaacgt
      841 tgcatatact gcgcttcatt gcgtcctgtt gctgtttcat tagccgtttc atgcagtaaa
      901 atatactgat tatttgctac ttgtgagcta ccttcatttg cgcccaaatt aaattcattg
      961 ttgatagtat aggcaaatcc gttaattggc aataaaaaaa gagccgttaa caggctcatc
     1021 gcagtaatag taattttctt tttcatatct atcctcctat ttatctgatg aaattttctt
     1081 atacgttcga tttgatacac ctaaaatact ccctagaaat gctccaaaac ctgtaataat
     1141 catcaccgtt atatctgtgt accgccagtt aacacctttt ccgacaagac cgacaaaagt
     1201 cgctaaagcg gggataacta ctatagcgat ccatttcaaa atatcaaagg ttctattgtt
     1261 catactatcc acctcccttc attggttggg ctactttctt tttcaatcgc ttgttttctt
     1321 ctatcaatat ttctctttct gaaagcaagg catcaatttg cccgtccagt tgttcaattt
     1381 tat
//
LOCUS       DACECY010000170         1366 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.184, whole genome shotgun sequence.
ACCESSION   DACECY010000170 DACECY010000000
VERSION     DACECY010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.184"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            82..>1366
                     /locus_tag="IWO38_002885"
     CDS             82..>1366
                     /locus_tag="IWO38_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAQ0314889.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLI"
BASE COUNT          466 a          263 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatca
//
LOCUS       DACECY010000171         1364 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.185, whole genome shotgun sequence.
ACCESSION   DACECY010000171 DACECY010000000
VERSION     DACECY010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.185"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="IWO38_002886"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="IWO38_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ0314890.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          393 a          242 c          216 g          513 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DACECY010000172         1345 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.186, whole genome shotgun sequence.
ACCESSION   DACECY010000172 DACECY010000000
VERSION     DACECY010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.186"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            123..1292
                     /locus_tag="IWO38_002887"
     CDS             123..1292
                     /locus_tag="IWO38_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0314891.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          482 a          218 c          240 g          405 t
ORIGIN      
        1 ttttacactc tcataaaaga tagcgtaaaa tattttgtgt aaatagaaat ttagggtaga
       61 aaaaaaagtc cattctgtaa aattaaagtt accacacaga aattttatag gaggactttc
      121 tcatgactaa atttactata gaaattatgc aaacactact caataaaggg gatttagatg
      181 aattatttca ggatcattta gagcgagcga ttaattcact tctacaagca gagctaactg
      241 cttttttaga ttacgaaaaa tatgatcgaa atggattcaa ttcaggaaat tcccgtaacg
      301 ggaattattc acgtacattt aaaacagaat atggcgagtt gaatttaact attcctagag
      361 atcggaatgg cgaattttct caacaaacac taccagcata taagcgaacc aatgattcat
      421 tagaaacgac cattatccaa ttatttcaaa aaggcattac tatggctgaa atctccaaat
      481 taattgaaaa aatgtatggt caccactata cgccacaaac aatctccaac atgagtaaat
      541 tggtggctga agatgtttta gcttttaaag aaagaacgtt agaagccaat tattccgtta
      601 tatttatgga tgcgacgcat atccctgtga agcgacagac tgtttcgaag gaagcagtgt
      661 atattacgat tggtattcgt ttggatggaa ccaaagaagt tcttgggttt actattgccc
      721 caactgagtc tgcttatatt tggaaagaag ttcttcaaga tcttagaaaa cgtgggttag
      781 aagaagtttt attagtagtg acagacggat taagctgtat tgaagaaagt atccatagtg
      841 tgtatccgaa tgcccaattt caacaatgtt gtgtgcatgt atctagaaat atcgctcata
      901 aagttcgtgt tcgaggtcga aaagaaattt gtgaggattt caaattggtt taccaagcga
      961 attcaaaaga agaggcattg gatcacatcg actttatgac taggaaatgg aaaaagcagt
     1021 atccaagagt cgtcaattta ctcttgaatc ctgccctatt aaccttttat aatttccctc
     1081 acgccatcag acgaacaatt tattcgacga acctgattga aggctttaat aagcagctaa
     1141 aacgatatac tcgaagaaaa gaacaattcc ctaatgaaga atctctagag agattcttca
     1201 tttctcaatt taatcaatat aaccaaaaat ttttaggtag aattcacaag ggatttaaag
     1261 aaattcagga tacattagag tcgatgattt aactgtaatt aacagaatgg attttccatt
     1321 tacacataat tcttgacgct acctt
//
LOCUS       DACECY010000173         1341 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.187, whole genome shotgun sequence.
ACCESSION   DACECY010000173 DACECY010000000
VERSION     DACECY010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.187"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(742..1083)
                     /locus_tag="IWO38_002888"
     CDS             complement(742..1083)
                     /locus_tag="IWO38_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0314892.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1083..1313)
                     /locus_tag="IWO38_002889"
     CDS             complement(1083..1313)
                     /locus_tag="IWO38_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0314893.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
BASE COUNT          411 a          224 c          197 g          509 t
ORIGIN      
        1 tgtattgata gtgtacctaa aagctctact gacataagat cgtgtaatcg gtaaagtaat
       61 tttattaatt atacgggatg taatcaatta gagaataaac taatcataaa aggtactttt
      121 acattcataa ttgtcgcttt gagattctac tttggttcac tatctgaccc aattgcacat
      181 tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt
      241 tttcagaatc tatgctaatt tctgtatgga tgttccagtg atggcaaatt tctcttttca
      301 ttgtttttct tgtcgcatga tatgcctttt tgtgttattc agttaaacta tagaaattct
      361 ttcctagaca gatcttgatc ccaaatttta cgtttttgag catcgatata gtttaaaatg
      421 acattactca tatctaaaga gtttagattt tttaggacta aaactgtcag ttgttctttt
      481 tcttctttct gttaaagtat tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa
      541 aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa atcacctcaa tcattcgttt
      601 aaaatgctat atattcttta gtttaagata cggaaattaa tgccatcgac cacactagta
      661 gaatgaacga catttacttg gccttttttt aatttcgtta tgtttagcat ataaaaatat
      721 tatagagttt acttacaatg cttaaaacat atactgtata atctgatcaa ttttagccat
      781 gtcttgcaat ggtaagtttt ctattttttt gagttttcgt tccttaaaat caagagattt
      841 taattgtgta ataattactt gtccttttat atctagctct tcaggtaatg agtaacgtgt
      901 cggtaaattc tttatagtgg aagtaattgg acatatgacc gaaaacattg ttttacggtt
      961 aaattcataa cgagagacga ctagtcctgg tcgtcttttt tgaatttctt ttcctacgga
     1021 gggatcaaaa tcaatccata caatatcacc ttttttagga atataaattc tttcttctga
     1081 cactataaaa tttctctccc ttcaggagtt aaatcttccc attcgtcttt ctcataatac
     1141 tcggcctctt ccccaccact aaatggatct tcaatttttg gaactaacgt aattgttcca
     1201 tcatcagaat acacaacgat atattcttgg ttttctgaag gtttctttcc gttatcagaa
     1261 ggtaaagtta ccactacaga acttccttgt aatctagatt ttgtaacaag cattatcatc
     1321 actcccttaa taaaagtata c
//
LOCUS       DACECY010000174         1314 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.189, whole genome shotgun sequence.
ACCESSION   DACECY010000174 DACECY010000000
VERSION     DACECY010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.189"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(190..801)
                     /locus_tag="IWO38_002890"
     CDS             complement(190..801)
                     /locus_tag="IWO38_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0314894.1"
                     /translation="MGIQIISLSDLLEVAPDLESVNSILKTFKSIPHPITGQVNDVEY
                     FLHQKAIEFEKAALATTHLLFSSYKDKSILVGYFSLANKSLIMSKKNYNNLSKPQQRR
                     LCQNGSKTETGGYIVNSYLIGQVGKNYSDEAQKIDAINGTQILTIAYDTVLQAKKIIN
                     ARYVWIECENIPRLIDFYRDFGFEVIENYETNSGLVVMVMKLK"
BASE COUNT          450 a          228 c          154 g          482 t
ORIGIN      
        1 taaatacttc atatcaatca tttattcaga aacaaactat cttgcaggat ctatacgagt
       61 taattacaga gcaaaaaagc ctcaaccaag gatgactggt tgaggttttt gttttacaaa
      121 tttaaatctt tttaagttca ctatacgctt gtttaacctt gtaatagata catgcaataa
      181 ttagttttac tattttaact tcataaccat tacaactaat ccactgtttg tttcataatt
      241 ttcaataacc tcaaaaccaa agtctctata gaaatcaatt aacctcggaa tattttcaca
      301 ttcgatccat acatatctag cattaataat tttttttgcc tgcaaaaccg tatcataagc
      361 tatcgtcaat atctgggttc catttatagc atctatcttt tgagcttcat cagaatagtt
      421 tttgccaact tgtcctatca aataactatt aactatgtaa ccaccagttt cagtttttga
      481 accattctga cacaatcttc tttgttgggg cttactgaga ttgttataat tttttttgct
      541 cataattaag gatttgttag ctaaagaaaa ataaccaact aatatagatt tatctttata
      601 tgagctaaat aaaagatgcg tagttgctaa agcggctttc tcaaattcta ttgctttttg
      661 atgcaaaaaa tattccacat cattaacttg accagttata ggatgtggaa tagatttaaa
      721 tgtctttagt atgctattta cactttctaa atccggagca acctctaata aatcacttaa
      781 actgataatc tgtattccca tctaactatt acttccaaaa aaaatatttt ttattttttc
      841 tttgtcatcg attactttta aattagttac caactgtttt ctaggtgatt tatcattaga
      901 taatgcatta attaaagagt tagcattctt ttttgtaaac ctatactcct cagtaaaact
      961 tttagtagcc ataacaatcg cctccgttta tgtaccaaca caaaaatata taggaaaaaa
     1021 cacgtcaaaa taacgtactt gtacaaaaca atcatacatc attttacgct ttaactttcc
     1081 tttgttgtaa gttcaatata ccatcattgg tttaattgta caagagaaac ggctaaaaaa
     1141 tcagctaatt ttgttagcaa ctttgttagc atatctgaca gtagatcaat agtaaaagtg
     1201 cctgtatata aggaaaaact gtatttttac tatctaaatt cgtaaacatt tttgcttaca
     1261 aattagctaa taatattagc aactatattg aaaactatat gtactgcccc tcac
//
LOCUS       DACECY010000175         1295 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.190, whole genome shotgun sequence.
ACCESSION   DACECY010000175 DACECY010000000
VERSION     DACECY010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.190"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(346..419)
                     /locus_tag="IWO38_002891"
     tRNA            complement(346..419)
                     /locus_tag="IWO38_002891"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(383..385),aa:Ile,seq:cat)
     gene            complement(974..1288)
                     /locus_tag="IWO38_002892"
     CDS             complement(974..1288)
                     /locus_tag="IWO38_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314895.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          477 a          202 c          195 g          421 t
ORIGIN      
        1 agtctttttc ccattatttt ttacagttat atggtatgct ttttaatggc ttcaaatata
       61 aaagagttta aagcgtaaca cacttatggg gaagtggttt ttggggaacg ctttaaactc
      121 ttctttatta ttatctcaca atataaccca aatgtctttc catttaaaaa tcaaagtaaa
      181 acttttcaaa tatacagaag tataactatg tgaaacatcc tttcattaat ccataaaagg
      241 atacataaaa aagccactca tttgagtggc aatgaagaaa agctttagct tgtataatac
      301 tcttcaaaaa attctaacac agaacgattc aattggctac attaatgtac cctgtaggac
      361 tcgaacctac gaccggacgg ttatgagccg tctgctctga ccaactgagc taagggtagt
      421 ggttgttgcc acataaagcc ataaacaatc aaccagtaga atgcgtggca acaaacctgt
      481 tatcgcatat cttggagtgt gactatttat gagtgatagt gaagatatgc gtctccatat
      541 atgtatggtt gagacagata aacatgtatg aaaaacgttg ttaaatcaac gtttttttat
      601 tttggtcaag gagagaaaag tagagaaata caaaattttt tgcattgatt ttgcattcaa
      661 aaaaacattc aaacatttag aaatccccaa tacgtattca ggaaaatcta ttttaaggtt
      721 tacaaaaaga acttttgttc gtacactctt tttgaggtga acttttatgc tgatggaagg
      781 aaaaacacaa ttatggttta agcatgttcc ttcaaataag ttttttaatt ataaactcta
      841 gagaaattaa ttcatcctaa cataaaataa ttgttcactc aaaagttttt ctagaacaat
      901 taaaacagca ggcttcacat tcgctataca tttgtaagaa gtaataattc ctgtactaaa
      961 aggcataatt gctttaatta ttttttgact tctcttcaaa cacaagaagt agttgtgtaa
     1021 taattgcaga aataaggatt ccaataatag atataaaatt tgacatggaa acgtctataa
     1081 cattaaaaga ataaagtgtt gaagaaagta aactgattga aataacccct ccgtaaaaaa
     1141 tagtctgtga atatacccac tttttttcag aagacgtact tcgtttggtt ctaaaaccta
     1201 tccctgcatt tctactctta tcaaaaaacc aaatatttag aagtaataat attgctatta
     1261 ttaagataaa attactgtaa aaatccaaaa gctat
//
LOCUS       DACECY010000176         1199 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.191, whole genome shotgun sequence.
ACCESSION   DACECY010000176 DACECY010000000
VERSION     DACECY010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.191"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            153..1199
                     /locus_tag="IWO38_002893"
                     /pseudo
     CDS             153..1199
                     /locus_tag="IWO38_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180785.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
BASE COUNT          489 a          136 c          228 g          346 t
ORIGIN      
        1 gttcttcgtg ttctcttaga cataaaaatt cctcctggta tgttttcttc tagtctacac
       61 accttaattt ttctgtctag ttaattgtag cctatccagt ttggaaaaat aaaaaattat
      121 atttcatcaa cgtttcataa aaggtgtgta gaatgttatt gaaagtagct ataaaatgct
      181 attgtgtaaa aaataaagga ggaataattt atgaaaaaat agtaccaaga ggagcatgcc
      241 tattacttgc attatgtttg agcttaagcg cagttgggtg tcaaaaaagt aatgaaaaca
      301 ataaaataaa agaaggacaa tccataagtt ctgaagagga aggcatgtca attaatgcaa
      361 atggcaaaaa tgaaactttt aagccatcag attatacatt ggaggcaaag aaagaatatg
      421 tttatgaata tttagggctt aaatttaaac tctcagacaa atttagaaat tatatagctg
      481 ataaaaagat agcgatgtta gatgatcaaa gtcctataga taaagaacta aagtatgcta
      541 ttttgacatt tgagaaaatg acagaagaac aaaagaatgc agttatagaa aagatgggcg
      601 atggatataa aaattggcaa aatgagcttg aaagaattgg gactattggt atatttgaaa
      661 aaaatacatc agaagaaaaa aatctaaaat tataaaatgt gatactcata ctaaaatagg
      721 agtttcaagt gatggaaaat acgattgcta tttaagcaca aatagcggag ctgaaagtaa
      781 tcttcttgat gaattaaaaa gaactgagat tcaaattata gataaaaaag aacgtcctaa
      841 aaatggtttt gtgctatcag aaaaaactga tttagaaaat acagaagcat ttaacaaaga
      901 atcggttaaa gatttacgta aactaccaac aaaggacata aatggcaagg attttacaag
      961 taaagatttt gagaagtatg atcttactat ggtaaatgta tttgcaactt ggtgtacagc
     1021 ttgtgtaaag gaaattcctg atttggttga ggttcagaat gaaatgaaaa gcaagggtgt
     1081 aaatatcgtt ggagttgtta cagatcctgt tgatgataac ggtgaaaaca aggaagcaat
     1141 tgaaaagtca aagttaatac atgaaaagac aaaggcttca tatcctttct taatgccag
//
LOCUS       DACECY010000177         1190 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.192, whole genome shotgun sequence.
ACCESSION   DACECY010000177 DACECY010000000
VERSION     DACECY010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.192"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            141..398
                     /locus_tag="IWO38_002894"
     CDS             141..398
                     /locus_tag="IWO38_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314896.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1043)
                     /locus_tag="IWO38_002895"
     CDS             complement(630..1043)
                     /locus_tag="IWO38_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAQ0314897.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          454 a          151 c          148 g          437 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatacc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca catataaaaa taaaaaaaca
     1141 tttgtatttt atcaaagttt tattatactt gtcaatttgt gtacatccta
//
LOCUS       DACECY010000178         1120 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.193, whole genome shotgun sequence.
ACCESSION   DACECY010000178 DACECY010000000
VERSION     DACECY010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.193"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1004
                     /locus_tag="IWO38_002896"
     CDS             <1..1004
                     /locus_tag="IWO38_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0314898.1"
                     /translation="KKLNTLVTIENPFRVKQFSRMFDENKTDRNDALRIADFLRIQRF
                     TTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHFLENLTYKCNTLAREMRDESTSLFS
                     ATLISLMTEDFTLDELAELPLEAFCDLLQEKGKGRFKQPEKIAKAIQRAITMSYRLGA
                     LAQESINVVLSVLVREIRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGL
                     IAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSV
                     KNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKFTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          400 a          219 c          195 g          306 t
ORIGIN      
        1 taaagaagct caacacactt gtaacgattg aaaatccttt tcgggtgaaa caatttagtc
       61 gcatgtttga tgagaataaa actgatcgaa acgatgcatt aagaatcgct gatttcttgc
      121 gtattcaacg attcacaact tcccctatca aagaagagaa atacatggcc ttacagcgtt
      181 taacacgtac tcgctatcaa cttattaagc aattgattcg tacaaagcaa cattttcttg
      241 aaaatctaac gtacaaatgt aatacgcttg ctcgagaaat gcgagacgaa tcgacgagtc
      301 tttttagtgc aaccctcatt tcactcatga cggaagattt cacattggat gaacttgctg
      361 agcttccttt agaagctttc tgtgatttac ttcaagaaaa aggaaaaggc cgtttcaagc
      421 aaccagaaaa aatcgctaaa gcaattcaac gtgcaattac catgagttac cgcctaggtg
      481 ctcttgcaca agaatcaatt aatgttgttt taagtgttct cgtacgagaa atacgagcac
      541 ttgaaaaaaa tatcaaagaa ttagataaag ccatcgaaca agtaattgtt gtcattccag
      601 aatatcaatg cttaaccagt attccaggcg ttggtaaagt ctatgctgcc ggtttaatcg
      661 ctgaaattgg tcaaatcgaa agatttgaag atcagacaaa attggcaaaa tacgctggat
      721 taagttggaa agtgaaccaa tctggaaact atcagtcgca aaatacacct ctgacaaaac
      781 aaggaaatcg atattttaga tactacttag ttgaagctgc caactctgta aaaaattatc
      841 ttcctgaata caaagctttt tatcaaagta aatataagga ggttccaaag catcaacaca
      901 aacgtgcact cgtcctaacc gcaagaaaat ttacgcgtct ggtggatacg ctactacgta
      961 aacaccaact ctatacgcca ccaaggagtg tgatagagaa ataacaatcc gttattgacg
     1021 caaatccttg aatcaacccg aaaaaaattt gattttgatc gggtctagtt tcgtgcgctt
     1081 caaaacatat tactcaataa agaaaatctt aaatttcatc
//
LOCUS       DACECY010000179         1104 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.194, whole genome shotgun sequence.
ACCESSION   DACECY010000179 DACECY010000000
VERSION     DACECY010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.194"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            102..>1104
                     /locus_tag="IWO38_002897"
     CDS             102..>1104
                     /locus_tag="IWO38_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0314899.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     "
BASE COUNT          336 a          214 c          219 g          335 t
ORIGIN      
        1 ggtgaacttc accaacatct tttttgtata aaaaaatata gaaaagacac cctgaaatcc
       61 tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat actcagctta
      121 tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat tgtttaacta
      181 aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg tacaagccag
      241 aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg gggacgacaa
      301 ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag ctgaagaaac
      361 aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct gttgctgaaa
      421 aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta aaagagaata
      481 cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc tatcgtgtca
      541 tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag gtcttatgct
      601 ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc atgatggacg
      661 gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat ttagaacgct
      721 atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt attgatatgt
      781 atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg atcatcgatc
      841 gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg aaagaaacca
      901 cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa ttgaagcgct
      961 attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg gtttggcgtg
     1021 ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta gctgcttgtc
     1081 ccaacttacg ccaaggctat cagt
//
LOCUS       DACECY010000180         1091 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.195, whole genome shotgun sequence.
ACCESSION   DACECY010000180 DACECY010000000
VERSION     DACECY010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.195"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            6..980
                     /locus_tag="IWO38_002898"
     CDS             6..980
                     /locus_tag="IWO38_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ0314900.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          384 a          135 c          187 g          385 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta a
//
LOCUS       DACECY010000181         1090 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.196, whole genome shotgun sequence.
ACCESSION   DACECY010000181 DACECY010000000
VERSION     DACECY010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.196"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..280
                     /locus_tag="IWO38_002899"
     CDS             <1..280
                     /locus_tag="IWO38_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ0314901.1"
                     /translation="KRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLS
                     QGGKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            623..879
                     /locus_tag="IWO38_002900"
                     /pseudo
     CDS             623..879
                     /locus_tag="IWO38_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
BASE COUNT          379 a          164 c          198 g          349 t
ORIGIN      
        1 taaacgtaat cccttcgtat ctgaatatgt taaacgactt gcaaaaggta tttgtcaact
       61 ttgccaagaa aaagggccat ttataaaaga cggtgtacct tatttacatt gtcatcatat
      121 agaatatttg tcgcaaggtg gaaaagatgt gattgaaaac tgtatcgcgt tatgtcccaa
      181 ttgtcatgct agaattcatg aacttgagtt acctgacgat aaagaaaaat tgctgaagat
      241 cgttgaaaaa agagaaaata gcttttctaa aataccgtaa tttattttta taggacaaat
      301 aagctaatgt gagctatgcc ttctatataa gactaagttc tattataagt aacgaagtaa
      361 ttctcgttcc ttcctgtagt aatatttttt aaataaccta taggcttctt caaaagtaca
      421 tcctccagta aattctaagt actggaggat tctttttcta gtgttgcttt actcatgaaa
      481 aagtagttgg ctacttcatc aaaatttctg aactttttta atgtataata aagagctcga
      541 tcatttacta caaggatgct agctcctaca ttttcacagt tttttagcaa gtaaattttt
      601 ctaaaatcac atcaggaaat gcatgagatt ctttaagaaa ggcaattata gggctatgat
      661 tacggttcgt gtaagtgacg ccaaaaaaga atggctttct tatatggctg aattttatgg
      721 gattacttta tctgaacttc taaaaaacta ttcgatggaa caactagaag atgaatacaa
      781 tcgtcaaacg gctgaaattg cccataaaca ttggataaga agtgggaaaa caacagtatt
      841 catggaagaa tccttaatga atttggtggg ctggaatgac ctgccgttta aaattcacgc
      901 ccgaaattca gaagcagctc tgattggcaa atacgtgtat tggtagagat agaagataat
      961 caattcgttg ttaccgctat tcaagtaggg caccgaagaa atgtctatga ttaagagact
     1021 gtttattttt acaaaaaaat aataactgga atctagttaa aactcttgaa gtgagagatt
     1081 tgactagatt
//
LOCUS       DACECY010000182         1061 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.197, whole genome shotgun sequence.
ACCESSION   DACECY010000182 DACECY010000000
VERSION     DACECY010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.197"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(36..992)
                     /locus_tag="IWO38_002901"
     CDS             complement(36..992)
                     /locus_tag="IWO38_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314902.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgtca a
//
LOCUS       DACECY010000183         1046 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.198, whole genome shotgun sequence.
ACCESSION   DACECY010000183 DACECY010000000
VERSION     DACECY010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.198"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1046)
                     /locus_tag="IWO38_002902"
     CDS             complement(<1..>1046)
                     /locus_tag="IWO38_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HAQ0314903.1"
                     /translation="HCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRT
                     FSAKTDLVDEHHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQ
                     SRMNFLPTVLCMDEFKSMKSVSGSMSFICVDGTTNQLFTILEDRRLYKLIQYFLRFPR
                     KARLKVKYLVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRT
                     SHSENQKKYRRLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELR
                     TAYHFCQLLRYYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSH
                     SNGVTEGLNNKIKL"
BASE COUNT          292 a          204 c          227 g          323 t
ORIGIN      
        1 atcagcttga tcttgttatt cagcccctca gtgacgccgt ttgagtggga caccttaaag
       61 gcatttgaaa tacccgattg gtagcaatgg aagatcgtga atttcttttt aaacgactga
      121 ggtaacgtag atgaaattgt tttaagggtt tcttgaaagc cttccgtacc tcgtttaacg
      181 aaataatagc gtaataattg acaaaaatga tacgcagttc gcaattcttc gttgtagctt
      241 aacagctcat caacgatttc tgtttgacta attggtcgtt taaacagtcg atgataatgc
      301 cgtttgagtg gctcccatgt cgtggaatct ttgagtaaga gtttccaata tcgcttgagc
      361 cgacggtact tcttctgatt ttcagagtgt gaggttcgaa aacgattcat gatctggaca
      421 cgtaattgat tgaagctgcg attgatgtgt tggacaatat gaaagcggtc agttacgatc
      481 tccgcatttg gaaagactgt tttaagcaac tgacaatagc tggcattcat gtccatcact
      541 aggtacttga cctttaaacg ggcctttcta ggaaagcgca ggaagtattg aattagtttg
      601 taaagtctgc gatcttcaag gatcgtaaat agctgattgg ttgtcccatc aacacaaata
      661 aaactcatgg aaccagaaac agacttcatt gatttgaact catccataca caacaccgtc
      721 ggaaggaagt tcatacgtga ctggtaatgc ttcgttgctt cacgcaagat ccgtgtcacc
      781 gtcccatccg agacgaagta cttcttagcg attagtttac gtgattgatt ttcgtacagt
      841 tccatgagaa ttgcctgctt cagttccttt gttagttgat gatgttcatc aactaagtcc
      901 gttttcgcag aaaaggtcct tccacaatca tggcagagaa aacgggtacg ttttaattct
      961 aaaatagtta acttttcttt gactttcaac atctgagtgc gtgtcgtata ggaaccgttg
     1021 cggataatac gaaccgatcg acagtg
//
LOCUS       DACECY010000184         1014 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.199, whole genome shotgun sequence.
ACCESSION   DACECY010000184 DACECY010000000
VERSION     DACECY010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.199"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..991
                     /locus_tag="IWO38_002903"
                     /pseudo
     CDS             1..991
                     /locus_tag="IWO38_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
BASE COUNT          342 a          201 c          197 g          274 t
ORIGIN      
        1 ccgtacggtc aagaccgagt acggggaact tcatctccag attccgcgcg accgcaacgg
       61 cgagttcaag caacagactg ttcctgctta tagacggacg aatgatatct tagaggagac
      121 cgtcattcac ctcttccgaa aaggtattac catgtcggaa atcgcagact tgattgagaa
      181 aatgtatggg catcactaca cgccccaaac catgtccaac atgactaaag tgctgactga
      241 agaagtaaat gcatttaaag ttagaactct aaatgataag tatgtcgcta ttttcatgga
      301 tgctacttat attcctttaa aacggcaaac cgtctccaaa gaagcaatct atattgccat
      361 tggtatacga gaagacggca ctaaagaagt actaagttat gcgattgctc cgactgaatc
      421 gacctacgtt tggaatgagt tgttacagga catttactcc agaggggttc atgatgtctt
      481 actgtttatt accgatggct taaaaggcat gaaagatacg attcaccaga tttatcctaa
      541 agctaaatac caacattgct gtgttcacgt ttcccgtaat attgctcata aagtacgtgt
      601 caaagatcga aaagaaatct gtgatgattt taaggctgtt tatcaagcta gctcaaagga
      661 agaggcaaat acctttttag ggagtatgat tgagaagtag caaaaaactt atcctaaagt
      721 gacgcagtca ctgataaaaa atcaagattt attgactttt tatgagtttc cgcctggtat
      781 ccgccgaagc atttactcaa ctaatttaat cgaatctttc aagaaacaaa tcaagaaata
      841 cagccgcaga aaagagcagt ttcaaaatga agaatcacta gagcgtttcc tagtatccat
      901 ctttgataca tacaatcaaa aattcttaaa tagaagccat aaaggcttcc aacaagtgac
      961 ggatacatta gcttcaatgt ttactgagta acccattatt ttgcagaagg acga
//
LOCUS       DACECY010000185          995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.200, whole genome shotgun sequence.
ACCESSION   DACECY010000185 DACECY010000000
VERSION     DACECY010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.200"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(24..911)
                     /locus_tag="IWO38_002904"
     CDS             complement(24..911)
                     /locus_tag="IWO38_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0314904.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          287 a          207 c          179 g          322 t
ORIGIN      
        1 atttctttgc ctttttatct aaatcaaacg tttagttctt gaccaactaa taaagaataa
       61 ctggacatag acatgccatt tttcgccatt agatcatcaa cagacatgcc aaatttttgt
      121 gcaatatccc atagcgtatc acctgacacg actgtatatg tctgaccatt atttgtattt
      181 acatctgttg ttgaagatga tgacgttgaa gtcgtcgtag tagtcgttgt tgttgtactt
      241 gtggttgttg tagccgaacc tgttgatggc gtatcaaaac gggttaaatt ataagtatta
      301 atcaatgagt tcaatttggc cccataattt ggatcggtag cataacgacc tgtcaagtac
      361 gctgttgcat cataaaaact tgttgtattg cttttccaca cgccagcata atgataatcg
      421 cccgtggaaa aactggttga acgaagaaca ttcgcatggt cttggaagga ttcccaataa
      481 gaagaatatt ttcggaatgg ctcattcatc gttacccatt gtccgtttac atattctttg
      541 gttgccatat aaacgacctg tccgttatag cttcctttca caccgaatag attataattt
      601 ggcgcactag ctaatccgga agtgccatat gcactttcca acaacgcttg agcgatcatg
      661 actgaagcat acaagtcatt acttgcagca acttctgacg cagaccagcc gatttgatcg
      721 ataaacgctt gaggatcagc gactgtttga gatccttgtt catctgcatg aacatgttgc
      781 ggtaccgctg taaccatcat cggcccaact aaaagactcg ttccaattaa aggagcaatc
      841 gcttttttat tgatcaccat ttttctttta ggtctatggt gccgggcact gcgggtcttc
      901 agctcttgca ttcaaattcc tcctcaaaat aaaaaaactt aataaaaagt tctcttctta
      961 aaacaaactt aattaattaa attatacttt taatt
//
LOCUS       DACECY010000186          957 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.201, whole genome shotgun sequence.
ACCESSION   DACECY010000186 DACECY010000000
VERSION     DACECY010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.201"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            34..>957
                     /locus_tag="IWO38_002905"
     CDS             34..>957
                     /locus_tag="IWO38_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0314905.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLS"
BASE COUNT          341 a          173 c          184 g          259 t
ORIGIN      
        1 ttccgacgaa agaattataa ggagtgagta caaatgacct ataaccatct tacaccaact
       61 gaactcgtaa tgatagaagc ttatttcaat caatcacaac ctgtttcaaa agtagccaat
      121 ctgttacaac gttctcgaca aactatttat aaggtctatc gttttcttaa atctggtgga
      181 agcgcaatga gctactataa acaatataag aaaaacaaac gtaactgcgg aagacatccg
      241 attgttttac ctgatgagca acaggaatac attcaaaaaa aggttgttca agggtgggct
      301 cctgatgtcc ttattggtcg agcagagttt cctattgctt gttcggtacg cacactgtat
      361 cgaatgttta aagataaaac ctttgacact cacgatttac caatgaaggg aaaaagaaaa
      421 ccaaacggtc ataaagaaaa aagaggaaaa caagggtttc gccggtcaat ccgagacaga
      481 aagacagact atacccagtt tgatcatgaa tttggtcacc tggaaggtga cactatcgtg
      541 ggtggaaaac ataaaagcgc ggtcatcaca ttagttgaac ggctatctaa agtcatcatt
      601 accttgaaac ccgaaggcag acaggctaaa gacattgaaa ataggctgaa tcaatggttt
      661 cgttgtgtcc caaaaaactt atttaaatcc atcacatttg attgtggaaa agaattctca
      721 aattggaaaa agattagtaa taccaacgat atttcaattt attttgcgga tccaggaaca
      781 ccttctcaac gaggattgaa tgaacattca aatggcttat tacgtaaaga tggtttgtat
      841 aaagctatgg attttaatgg tgtatctgaa acctttatcc aatcggtggc atcgaaacga
      901 aatcatattc ctagaaaatc attacactat cggacaccaa tggaagtatt tttgagt
//
LOCUS       DACECY010000187          927 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.202, whole genome shotgun sequence.
ACCESSION   DACECY010000187 DACECY010000000
VERSION     DACECY010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.202"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            16..>927
                     /locus_tag="IWO38_002906"
     CDS             16..>927
                     /locus_tag="IWO38_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ0314906.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNHTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
BASE COUNT          298 a          181 c          193 g          255 t
ORIGIN      
        1 ttttaggagg cccatatgat tatctcgaca attacagaag ctattggttc cacaccgctt
       61 tatcgctttg acaatcagca ctattctatg cctaaaggtt ctgctattta tgcaaaacta
      121 gaatatttaa atcctggagg aagtatcaaa gatagattgg gcagttattt gatcaaagaa
      181 ggatttgccc aaggaaaaat caatgaaaaa acgacgatca tcgaaccaac tgccggaaat
      241 acaggaattg ggatcgctct ggccgcactc acatacaaat taagaacggt ttttgttgtt
      301 ccagagaagt ttagcttaga aaaacaacaa ttgatgaaag cattgggagc aaagatcatc
      361 cattctccta gtgagcaagg aatcatcggt gccatcagca aaagtaaaaa acttgcagag
      421 gagatatcaa acagctatct gcctcttcaa tttgaaaata aagataatcc tgctgcttac
      481 tatcatacgt tagggccaga aatctttcat gaaatcaaag aaaatatcca tagttttgta
      541 gcaggaattg ggagtggtgg aacctttgct ggaacttcta cctttttgaa agaaaaatat
      601 cccgacattc gaataatcgg tgtagaacca gagggctctg tgttaaatgg tggtgatcca
      661 gcccctcacg aaattgaggg tatcggtgta gaattcattc cgccgtttct ttctcccctt
      721 tcaatcaatc agatcgagac aatctctgat gtagaagggt ttaaccatac taggcagtta
      781 gcacgagaac aaggactatt agttggaagt tcgagtggtg cggcttttgc agcagcatta
      841 agagaaattc gtcgtttacc acctggtcat cgtgtcgtta cgatttttcc agacgcagca
      901 gaccgctatt tatcaaaaaa catttac
//
LOCUS       DACECY010000188          863 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.203, whole genome shotgun sequence.
ACCESSION   DACECY010000188 DACECY010000000
VERSION     DACECY010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.203"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..861
                     /locus_tag="IWO38_002907"
     CDS             <1..861
                     /locus_tag="IWO38_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ0314907.1"
                     /translation="EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPD
                     KYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKR
                     YKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIIS
                     YSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMS
                     RKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLS
                     PVEYRKQSIK"
BASE COUNT          374 a          112 c          140 g          237 t
ORIGIN      
        1 gagcaagaag aaatcaaggt taaaacaatc cttcagctca aaaaagaatt taaattgaat
       61 ctattacttt caattgcaca attagcgaag tcaacttatt attactgggt aaaaaaatta
      121 gataagccag ataaatatag caagataaag caagaaatta cagcgattgt aaaagaatca
      181 agaaactctt atggttatcg tagagtcact ttagcgttaa agatgaaagg atatacaatc
      241 aatcataaaa cagttagaaa attaatgtcc caaatgggac ttacctgtca aatccgaata
      301 aagcgatata aatcttataa aggaacagta ggaaaaattg ccaagaatgt gttaaaacga
      361 aatttttcag tagatacacc caataaaaaa tgggtgacag acgtcactga attcaagata
      421 aaaggaagaa aaatctatct atctcctata cttgatttat ttaacggaga aataattagt
      481 tatagtatat caactagtcc aacatataaa ctgattgaag aaatgcttca acaagcaatt
      541 aaaaaaaagg gaactgaggg ttcactgatt ctacattcag atcaaggatg gcagtatcag
      601 atgccacaat atcaaaaaaa attaaaagag aataacatta ttcaaagcat gtctagaaaa
      661 ggaaattgct tagataattc tgtaatagaa aatttttttg gtgtgcttaa atcagagttt
      721 ttttatcgag aaaaattccg atcaattgag atatttcaaa gtaaattaaa tgagtatatt
      781 aggtggtata ataacaaaag gataaaacta aagttaaatg gactatctcc agtagaatat
      841 cggaaacagt ccattaaata gtt
//
LOCUS       DACECY010000189          835 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.204, whole genome shotgun sequence.
ACCESSION   DACECY010000189 DACECY010000000
VERSION     DACECY010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.204"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>835
                     /locus_tag="IWO38_002908"
     CDS             <1..>835
                     /locus_tag="IWO38_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0314908.1"
                     /translation="KFNNHSYGFRPNRSAEHAIADCMARMNQSHMEYVVDVDIKGFFD
                     EVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVLPNGKLIYPTKGTPQGGILSPLLA
                     NINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDKGNIYHKLRKSTTLKEIYLVRYAD
                     DFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEKSKITNLSKEESEFLGFTMKLVTK
                     GNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNSKDTFIYIGVYNSMVIGIHNYFKI
                     AT"
BASE COUNT          339 a          133 c          154 g          209 t
ORIGIN      
        1 aaaattcaat aatcacagct atggtttccg cccaaataga tcagcagaac acgcaatagc
       61 agattgtatg gcccgaatga atcaaagtca catggaatat gtggtagatg tagatatcaa
      121 aggtttcttc gacgaagtca atcatactaa actcatgaga caaatctggt ctatgggtat
      181 acgagacaaa cagcttctct taatcattag gaaaatgtta agagcacccg tagtacttcc
      241 aaatgggaag ttgatatacc caacaaaagg aacaccacaa ggcggaatac ttagcccatt
      301 acttgcgaac atcaatctca acgaatttga ctggtggata gcaaatcagt gggaggaaaa
      361 agatgccaaa gagctaaaac atcattttag gaaaaatgga gcttgggata aaggtaatat
      421 ctatcacaag ctcagaaaga gtacaactct aaaagaaata tacctagtga gatacgcaga
      481 tgattttaaa attttctgta gaaattataa tgaagcgaaa aaagttttct atgcaactaa
      541 aatgtggtta caggagcgtc taaagttacc aatatctgaa gaaaagtcaa aaatcacaaa
      601 tctttcaaaa gaagaaagtg aatttttagg ctttacgatg aaattagtga ccaaaggtaa
      661 caaaaaggtt gttaatacgc atataggtac aaaagcacaa agcatgatta aagtgaaact
      721 aaaaaaacaa atcaaacgaa ttcaaaaaag tcctaatagc aaagatacct ttatctatat
      781 tggagtctat aacagtatgg ttattgggat tcataattac ttcaaaattg cgact
//
LOCUS       DACECY010000190          801 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.205, whole genome shotgun sequence.
ACCESSION   DACECY010000190 DACECY010000000
VERSION     DACECY010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.205"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            129..>801
                     /locus_tag="IWO38_002909"
     CDS             129..>801
                     /locus_tag="IWO38_002909"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0314909.1"
                     /translation="MSMNHFIKQLFTIKDSNIQIQSFEETFYKSRKSIVIEGTLIRTY
                     RRCPCCKDSTKQIVKNGKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLV
                     TPFCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPR
                     CLLFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHY
                     IVTDMN"
BASE COUNT          240 a          153 c          147 g          261 t
ORIGIN      
        1 gctctataat ttctaggact gatggaaatt ttctatctgt cctagttttt ttgtaaaaaa
       61 acaaaaagac atacaaaatt tccggtagaa tagagtctcc tacaacatca ttctggggga
      121 attcttgtat gtccatgaat cattttatca aacaactctt tactattaaa gactcaaata
      181 tccaaattca atcctttgaa gaaacttttt ataaaagtag aaaaagtatc gttattgagg
      241 gaactctgat ccgtacctac agacgttgtc cgtgttgtaa agattctaca aagcaaatcg
      301 ttaaaaatgg caagaagatc tctatgattc tcctgaatcg tagcggtaat aaacggacct
      361 atcttcggtt gaagaaacag cgatacctct gccgagcatg taaaaaatat tttaccgctc
      421 ggacgtatct cgttacccca ttctgcttta tttctaagca gattcattac aagatcttag
      481 aagaattgac agagcgtcaa tctataaaag caattggtaa acattgcgat gtttctgtga
      541 caactgttca gcggacattg tccgccttgt catcagatat ccgtcagaat cttgagtatt
      601 tacctcggtg tctattattc gatgagtttc gttctctttc tacatggcac ggtaaatttt
      661 ctttttcttg tatggacggt gagaccggga aactgtttga gatcttacct agccgccgta
      721 aaaaagatct cgttgctttt ttcatgcgtt tttctctcaa agcgaggttg ggtgtacatt
      781 acatcgtaac ggatatgaac g
//
LOCUS       DACECY010000191          791 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.206, whole genome shotgun sequence.
ACCESSION   DACECY010000191 DACECY010000000
VERSION     DACECY010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.206"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..782
                     /locus_tag="IWO38_002910"
     CDS             <1..782
                     /locus_tag="IWO38_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0314910.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          107 c          102 g          242 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc t
//
LOCUS       DACECY010000192          765 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.207, whole genome shotgun sequence.
ACCESSION   DACECY010000192 DACECY010000000
VERSION     DACECY010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.207"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..727
                     /locus_tag="IWO38_002911"
     CDS             <1..727
                     /locus_tag="IWO38_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAQ0314911.1"
                     /translation="LLIGKPENTAIWEESLGKIAQGSYTQEAFLRNIYNYINKTVSDL
                     KKVEFTSDYLEKTVNQNKFDIGSYQVEEKKKVYSILNITSGEQFVIFKNISGKAINKK
                     VLQELLLNNRTDKVLSGFKSKEGKAFNARLLFDPNVMKVVMEFEKNSPKGDKKSKHDT
                     VNGYEIVEKAKVFEIKELATGESFIFYKNNSGKTLSSKTVKELLENGKTKEKVTGFIS
                     KDNKKYSAYLVFDNKNKVIKKEF"
BASE COUNT          329 a           78 c          135 g          223 t
ORIGIN      
        1 cctattgatt ggaaaaccag aaaatacagc gatatgggaa gaatcacttg ggaaaattgc
       61 gcaaggtagc tatacacagg aagctttttt aagaaatatc tataactata tcaataagac
      121 tgtttcagat ttgaaaaaag tagaatttac ttcggattat ttagaaaaga ctgtaaatca
      181 aaataaattt gatataggta gttatcaagt ggaagaaaag aaaaaagttt attctatttt
      241 aaatattact agtggtgaac agttcgtgat atttaaaaat atatcaggaa aagcaatcaa
      301 taaaaaagtt cttcaagaac tattactaaa taatcgtaca gataaagtcc tttccggctt
      361 taagtcaaaa gaaggaaaag cttttaatgc aagattactt ttcgatccaa atgtgatgaa
      421 agtggtaatg gagtttgaaa agaacagtcc aaaaggcgat aaaaagagca aacacgatac
      481 agtaaatggt tatgaaatag ttgaaaaagc aaaagtattt gaaataaaag aattagcaac
      541 aggtgaaagt tttattttct ataaaaataa tagtgggaaa actttaagtt caaaaacagt
      601 caaagaattg ttagaaaatg ggaaaactaa agagaaagta acgggtttta taagtaaaga
      661 caataaaaaa tatagtgctt atcttgtctt tgataataaa aacaaagtta taaagaaaga
      721 attttaattc ttagtggcgg aaatcatttg tttttccgct tattt
//
LOCUS       DACECY010000193          735 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.208, whole genome shotgun sequence.
ACCESSION   DACECY010000193 DACECY010000000
VERSION     DACECY010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.208"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            101..631
                     /locus_tag="IWO38_002912"
     CDS             101..631
                     /locus_tag="IWO38_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314912.1"
                     /translation="MISDNKWEINLKRFISEYIYTIETKSSFSLNEDSDPLKKYTIQC
                     KKNKDEKLFVIRPDLMKSNTHYIKEKPKDCDYIIISEANKTVFLIELKSSAQTSPAKE
                     ISEQIDGGYKWLQHLSFLTKYDFTEFSYKIIKVCCKFEARQDRKNLFKPNEYGIYNMN
                     GPKLDLRELYRKCNDL"
     gene            complement(626..>735)
                     /locus_tag="IWO38_002913"
     CDS             complement(626..>735)
                     /locus_tag="IWO38_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0314913.1"
                     /translation="NIVKDLNEGVSISKIAKNYNVTRQTIYRIKKDLNL"
BASE COUNT          296 a           86 c          111 g          242 t
ORIGIN      
        1 aaatttctaa catcagttat tatgataatt ttgatgatgt caattatgat ttacaggaaa
       61 gatataatga tttaatcgaa gaattagagg agagaggaga atgatttcag ataataaatg
      121 ggaaataaat ctcaaaagat ttatttcaga atatatatat acgattgaaa ctaaatctag
      181 tttttctctt aatgaagaca gtgatcctct aaaaaaatac actattcaat gtaagaaaaa
      241 taaagatgaa aaattgttcg taataagacc agatcttatg aagagtaata cccactatat
      301 aaaagaaaaa cctaaagatt gtgactacat catcataagt gaagcaaata aaacagtatt
      361 tctgatagaa cttaaaagct cagctcagac ttcccctgca aaagaaatta gtgaacaaat
      421 tgatggtgga tataagtggt tacaacatct tagttttcta acaaaatatg attttactga
      481 attcagttat aaaattatta aggtatgttg taaatttgag gcaagacaag atcgtaaaaa
      541 tctttttaaa cctaatgaat atggtattta taatatgaat ggtccaaaat tagacttaag
      601 agagttgtat agaaagtgta acgatctata aattcaaatc ttttttaatt ctatatattg
      661 tttgtctagt aacgttataa ttctttgcaa tctttgaaat agagacgccc tcattcaagt
      721 ccttaacgat gtttt
//
LOCUS       DACECY010000194          679 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.209, whole genome shotgun sequence.
ACCESSION   DACECY010000194 DACECY010000000
VERSION     DACECY010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.209"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          240 a          114 c           80 g          245 t
ORIGIN      
        1 ccaaattact tcatcaaaac attgatataa tttttattat atcagtattt attcttgata
       61 cataaatgag aaaataaata ctttaaatct attgactaca agtagtcatt aagtcttcat
      121 aagatgtaca atatgacatt aaaaaaataa aagcttacga ccatccgtta aatctgacac
      181 taaataatgg actgctttct tttgtattag caggttacgt tgactatgaa tattcaccag
      241 attatttgga gaaacctctt caacacgaga acaatgatat taattttaga tttgtctacg
      301 agtcaaaaag taaaacatgg ttagttgatc aattaagcac gataaacaca agcttccatg
      361 aattacctaa acctgaaaat gaagttgaga tggtcattaa aatcttctaa aaaagaagcg
      421 ttgaagggga aatattcctt tcaacgctct tttttatatg cttagaaaca taaataaatt
      481 gtatactttc tttttagccc ctattacttt tcacgaaaaa tatctttttt tcgacgctag
      541 cgtttctcta cttctaatac tgtacacagt ttttgtatta ttgaaaaatt actcattatt
      601 tagcattaat ccacatcgtt ttatcaatcg accttcatac taaactactt taaagtcatc
      661 ttccttctcg tttttcatt
//
LOCUS       DACECY010000195          674 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.210, whole genome shotgun sequence.
ACCESSION   DACECY010000195 DACECY010000000
VERSION     DACECY010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.210"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            281..>674
                     /locus_tag="IWO38_002914"
     CDS             281..>674
                     /locus_tag="IWO38_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0314914.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQ"
BASE COUNT          251 a          101 c          159 g          163 t
ORIGIN      
        1 gcaaaagcta gcatctaagt taaggcatgt ggatagatat aagggaacag gggaagccat
       61 caacatggag agtgcatact cgatgaagtg gtagtaagaa atatgtgaat gacaaataac
      121 ttactttagt gatggtgaaa gtagagtcac gactgatgaa ggttctgtaa tggaactgga
      181 ggaacaggct cgattcgtct gtaaaagtca tgaaacccgt tcaaccatgg gattctcgta
      241 tgactaaaaa ggtcacgagc caattggagc gtgataaacc atgacaaaag aaacaagtcc
      301 aaaagatagg aagttgcggc atgcagaata ttatggcatg acggaaatct tcgataagat
      361 atactccaaa agtttgaagg gagataattt caagaatcta atgcagatta tcatttctga
      421 taacaacatt gtcctagcat atcgtaatat caagagaaat cacggtagtg tgacaagagg
      481 agaagatgga gtaacgatta aagatattga aaatctcaac cgtaatgaat tcgtagaaat
      541 tgttaggaag cgattcgcac attataaccc tcggaaagta aggcgtgtag aaattcctaa
      601 atataatggt aaattaagac cgttgggtat tccatcaata tgggatagaa tcgcgcagca
      661 atgcatctta caag
//
LOCUS       DACECY010000196          668 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.211, whole genome shotgun sequence.
ACCESSION   DACECY010000196 DACECY010000000
VERSION     DACECY010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.211"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>668
                     /locus_tag="IWO38_002915"
     CDS             <1..>668
                     /locus_tag="IWO38_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HAQ0314915.1"
                     /translation="AEEFIRSRKAYGSRKIKKALSKRGIQISRRKISRIMKNRGLKSS
                     YTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLF
                     NREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDIN
                     RSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTL
                     GYET"
BASE COUNT          233 a          117 c          128 g          190 t
ORIGIN      
        1 cgctgaagaa tttatccgca gccgaaaggc ctacggctca agaaaaataa aaaaagcctt
       61 atcaaaacga ggcattcaga tcagccgacg aaaaattagt agaatcatga aaaatagagg
      121 attaaaatcg agctatactg ttgcttattt taaagtacat cattctactt gcaatgaagc
      181 caaaacgaca aacgtattga atcgtaaatt cttaagagac aacccattag aagcgatcgt
      241 aacagacttg acttatgtac gagtcgggaa aaaatggaat tatgtctgtt tcattttgga
      301 tctgttcaat cgagaaattc tcggctattc ttgtggagaa cataaagatg ccgttctagt
      361 aaaaaaagca tttagccgta tcaaacaacc tctgacagag gttgagattt ttcatactga
      421 tcgtggaaaa gagtttgata accaagctat tgatgaatta ttaacaactt ttgacatcaa
      481 tcgatcattg agtcataaag gctgtccttt tgataatgcc gtagctgaat caacttataa
      541 gtcgttgaaa gtagaatttg tctatcaata cacatttgaa accttacaac aattggattt
      601 ggagttattt gactatgtca attggtggaa ccaccttcgg ttgcacggta cacttggcta
      661 cgagacac
//
LOCUS       DACECY010000197          631 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.212, whole genome shotgun sequence.
ACCESSION   DACECY010000197 DACECY010000000
VERSION     DACECY010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.212"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            28..>631
                     /locus_tag="IWO38_002916"
     CDS             28..>631
                     /locus_tag="IWO38_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0314916.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDI"
BASE COUNT          216 a          114 c          123 g          178 t
ORIGIN      
        1 ccaaagaatt ataaggagtg agtacaaatg acctataccc atcttacaac ggatgaactt
       61 gtaataatag agtcttattt caaaatgaat caatctgttg ctaaaactgc ccattgcttg
      121 aatcgttcaa gacaaacgat ccataaagta tatctgttct tcaagcaagg aaaatcagcc
      181 ttagaatatt atcaacagta taagaaaaac aaatcaaact gtggtagacg tccgcttgtt
      241 ttacccgagg aacaatcaga atatattcaa agaaaggttg ttcaaggatg gacacccgat
      301 gtgattgttg gtcgtgcagc gtttcctatt agttgttctg ctcgtaccat ttatcgtatg
      361 ttcaaaaagg ggctatttga ttcttctgac ttaccgatga aaggcaagcg taaaccgaat
      421 ggacatcaag aaagacgtgg aaaacaaact ttccgccgct ctattcatga acgtgaaaag
      481 gattatagcc aattctcaaa tgagtttggt caccttgaag gtgacactat cgtaggtctg
      541 aaacataaaa gtgctgtaat taccttagtt gaacgattat caaaagttat catcacattg
      601 aaaccgtgtg gtagacaagc gattgatatt g
//
LOCUS       DACECY010000198          627 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.213, whole genome shotgun sequence.
ACCESSION   DACECY010000198 DACECY010000000
VERSION     DACECY010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.213"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            330..>627
                     /locus_tag="IWO38_002917"
     CDS             330..>627
                     /locus_tag="IWO38_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0314917.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFP"
BASE COUNT          230 a           69 c          104 g          224 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaa
//
LOCUS       DACECY010000199          605 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.214, whole genome shotgun sequence.
ACCESSION   DACECY010000199 DACECY010000000
VERSION     DACECY010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.214"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            165..371
                     /locus_tag="IWO38_002918"
     CDS             165..371
                     /locus_tag="IWO38_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314918.1"
                     /translation="MVKFPQFKLNPAASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQ
                     ISSNLEDIKKAFKIKTIGTAPNFK"
BASE COUNT          261 a           76 c           67 g          201 t
ORIGIN      
        1 aattatatgt ttttaatagt ttagatgttg aaatacctat gaaacagatg aaaaatattg
       61 acatacatat actgccaagt gagtcgtgcc aaatagaata ttcaatcgaa tatttttaca
      121 aaggtaaaaa aataaaaaaa tgtgacaagt gttttgaatc agctgtggta aagtttcctc
      181 aatttaaact aaatccagct gcctcgatac aatttcaagg acatgtattt gatgttttag
      241 ctaaagataa cataaaaaaa tacaaaaaag gtaatatatt ccatattgat atacaaatat
      301 cttctaattt agaagatata aaaaaagcct ttaaaattaa aacaattggc actgctccta
      361 attttaaata acaaaaaaat tactagataa tctaaaaaca ggatggcatt atgagaacaa
      421 taactaaact tcctatatca catattttgt aattagtgaa aagcaaattg actactatca
      481 taagttaatg actctcatct taatatttaa aatatattca tatttttcta ttttatctaa
      541 atgcgatttt catatctaat aatagaaaaa taaacaatta acaataaaaa agtcttcatt
      601 tattg
//
LOCUS       DACECY010000200          548 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.215, whole genome shotgun sequence.
ACCESSION   DACECY010000200 DACECY010000000
VERSION     DACECY010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.215"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>548)
                     /locus_tag="IWO38_002919"
     CDS             complement(<1..>548)
                     /locus_tag="IWO38_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0314919.1"
                     /translation="SLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQW
                     ENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPT
                     IFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEG
                     NDDLLEKYMSGKSLEALELEQE"
BASE COUNT          147 a          119 c           84 g          198 t
ORIGIN      
        1 tcctcttgtt cgagttccaa tgcttctaat gatttaccgg acatatattt ctctaaaagg
       61 tcatcgtttc cctctattac cgtatcccat tgttcagatt cggtaaagtt cgtcacacac
      121 atattaggat acagttctac cttctgtttg attacaattt cggcagaaag tttctcttta
      181 atatcctgat aaaccgttga taaatcaatt ccattttggt caatcttatt gataaaaaag
      241 attgtgggaa tccccatttt cctaagtgca tgaaataata tacgagtttg tgcttgtacg
      301 ccatcttttg cagaaatcag tagaattgcc ccatctaaaa ctgataatga acgatatact
      361 tctgctaaga aatccatatg tcctggcgtg tctatgatgt tcaccttcgt attttcccac
      421 tgaaaagagg ttattcctgt ctgaattgta attcctctct gacgttctaa aagcgtatta
      481 tccgtcctcg ttgtaccttt gtccacgctt cctaattctg taatcgctcc actgttatat
      541 aataagct
//
LOCUS       DACECY010000201          543 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.216, whole genome shotgun sequence.
ACCESSION   DACECY010000201 DACECY010000000
VERSION     DACECY010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.216"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          209 a           87 c           52 g          195 t
ORIGIN      
        1 atgaaatacc tctttgttac tcataattat accataatta gtcacgaatg caaccaatag
       61 aaaaaaatac ttagaacggt agatcatcgc taataacaat gcattgtcca ccaaatggat
      121 tttgtgaagc attaaataca ttgttattaa ttgtcgctgc ttttaactag gcctctcccc
      181 ttctattata aaagaagata tatattattt cttaattacc tactacctta aaccaaaatt
      241 taagtactac ctaatttaag tagtacttaa ataatctaaa aaatagttat cccaattgtt
      301 atgcttttat aatacgatta tttgtaacaa aatgaatatt atataattaa acactaccat
      361 atttaggtag tgtttaatta ttagcaaaac aaaaaatatt tattctattc tcttaacttc
      421 aactaagtta tgctgcacac ctttatataa atcatatgca actcattagc tagacaaact
      481 acaagcatgt agttttatct gttaattatg tactacctaa ataaattagt ttataattaa
      541 caa
//
LOCUS       DACECY010000202          528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.217, whole genome shotgun sequence.
ACCESSION   DACECY010000202 DACECY010000000
VERSION     DACECY010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.217"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(5..90)
                     /locus_tag="IWO38_002920"
     tRNA            complement(5..90)
                     /locus_tag="IWO38_002920"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="IWO38_002921"
     tRNA            complement(107..178)
                     /locus_tag="IWO38_002921"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="IWO38_002922"
     tRNA            complement(190..262)
                     /locus_tag="IWO38_002922"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="IWO38_002923"
     tRNA            complement(280..361)
                     /locus_tag="IWO38_002923"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="IWO38_002924"
     tRNA            complement(369..441)
                     /locus_tag="IWO38_002924"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="IWO38_002925"
     tRNA            complement(445..517)
                     /locus_tag="IWO38_002925"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACECY010000203          513 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.218, whole genome shotgun sequence.
ACCESSION   DACECY010000203 DACECY010000000
VERSION     DACECY010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.218"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            25..>513
                     /locus_tag="IWO38_002926"
     CDS             25..>513
                     /locus_tag="IWO38_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ0314920.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMD"
BASE COUNT          188 a           93 c           85 g          147 t
ORIGIN      
        1 attcacagaa aagaggactt ccctatgaat gattttacta cagaaattgt gcaaactcta
       61 gtcactaaag gcgatttaaa tgaattattc cgttcgcact tagaaaaagc gataaacaca
      121 ctcctacgga ctgaattaac ggctttttta gattacgaaa aatatgatcg cactggtttt
      181 aattcaggta attcgagaaa cggttcttac tttcgatcaa tcaaaaccga atatggtgaa
      241 ttaacattgg aaatacctag agatcgtaat ggtgagttta aacaacaaac tttaccagcc
      301 tacaaaagaa caaacgatac attggaaacc actattatcc atttattcga aaaaggtgtt
      361 acgatgtctg aaattgctga tttgatcgaa aaaatgtacg gtcatcacta tactccacaa
      421 accatgtcca acatgactaa agttctgact gaagaagtaa atgcctttaa atccagagcc
      481 ttaaatgata agtatgtcgc tatttttatg gac
//
LOCUS       DACECY010000204          513 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.219, whole genome shotgun sequence.
ACCESSION   DACECY010000204 DACECY010000000
VERSION     DACECY010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.219"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IWO38_002927"
     CDS             complement(<1..132)
                     /locus_tag="IWO38_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0314921.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          185 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag aca
//
LOCUS       DACECY010000205          500 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.220, whole genome shotgun sequence.
ACCESSION   DACECY010000205 DACECY010000000
VERSION     DACECY010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.220"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>500
                     /locus_tag="IWO38_002928"
     CDS             <1..>500
                     /locus_tag="IWO38_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314922.1"
                     /translation="SLYPVYHGSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFK
                     IEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEI
                     VILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDS
                     DPLLQY"
BASE COUNT          179 a           82 c          100 g          139 t
ORIGIN      
        1 tccttgtacc ctgtttatca tggaagcgca aaaagcaaca tagggattga gcagcttata
       61 gaagtgataa cgaataaatt ttattcatca acatacagaa agaagtctga actttgcgga
      121 aatgtcttca aaattgaata ttcggaagaa agacaacgtc ttgcatatgt acgcctttat
      181 ggcggaatcc tgcatttgcg ggattcggtt agaatatcgg aaaaggaaaa aataaaaatt
      241 acagaaatgt atacttcaat aaatggtgaa ttatgtaaaa ttgataaggc ttattccggg
      301 gaaattgtta ttttgcaaaa tgagtttttg aagctaaata gtgttcttgg agatacaaag
      361 ctattgccac agagagagag aattgaaaat ccgctccctc tgctgcaaac aactgttgaa
      421 ccgagcaaac ctcaacaaag ggaaatgtta cttgatgcac ttttagaaat ctccgacagt
      481 gacccgcttc tacaatatta
//
LOCUS       DACECY010000206          461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.221, whole genome shotgun sequence.
ACCESSION   DACECY010000206 DACECY010000000
VERSION     DACECY010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.221"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..436)
                     /locus_tag="IWO38_002929"
     CDS             complement(<1..436)
                     /locus_tag="IWO38_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0314923.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAEN"
BASE COUNT          131 a           92 c           59 g          179 t
ORIGIN      
        1 cgttctctgc tcttaaacga ataacttctt cttcaagaga ctcatcttta agttttttag
       61 gaatgtttag cttggaattc atactaattt ttctgcccct tttttggctc tcaagtgaag
      121 aagcaccgcc ttcttcatat tgctctatcc atttacttag agttctattt gaaccgatat
      181 taaatttttt agcagtttct tggatagaaa gaccatttgt ttccatatat tctataacat
      241 caagtttaaa ttttgtagtg tagcttttgc cacctccaac caagccttcc caaccatgat
      301 agttataaat ccttacccaa tgtctaacca gtgtacgatt tatttgatat ttatgtgcaa
      361 gatatttgta gccgccttcg ttatttaaat agtctgaaac tacttttttc ttaaaaacaa
      421 atgtatattt ccgcaaaaaa agcacccctt ttaattagat t
//
LOCUS       DACECY010000207          453 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.222, whole genome shotgun sequence.
ACCESSION   DACECY010000207 DACECY010000000
VERSION     DACECY010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.222"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>453
                     /locus_tag="IWO38_002930"
     CDS             <1..>453
                     /locus_tag="IWO38_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0314924.1"
                     /translation="IARELRPSFEIRLREQGISKEGQYGKYEGYKPYLRSGQIRYIRG
                     FPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTVEEWKISWLCSNPVLSKRAT
                     IEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYHESKSDSYEN"
BASE COUNT          182 a           74 c           96 g          101 t
ORIGIN      
        1 aaattgcaag agaactcaga ccaagttttg aaatacgact acgagaacaa ggaatctcaa
       61 aagaaggaca atacggaaag tacgaaggat acaagcctta cctacggtca ggacaaatac
      121 gatatataag agggtttcca atattaccag tagggtatat tcaacacaga catccgatgt
      181 gtaaaaaaag aaatattaat aaatacaccg aagaaggtag ggaagccatt cataagaagc
      241 aacaaaccgt agaagaatgg aagatatcgt ggttgtgtag caatccagtg cttagtaaga
      301 gagcaacgat tgaatttgcg gacaatcgaa tttcaaaatt catcgctcaa aagggaagat
      361 gtgcagtaac aggtgaggag cttatcttat ctgaaatgca ttgtcatcat attatccctt
      421 atcatgaaag caaaagtgat agctatgaaa acc
//
LOCUS       DACECY010000208          452 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.223, whole genome shotgun sequence.
ACCESSION   DACECY010000208 DACECY010000000
VERSION     DACECY010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.223"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>452
                     /locus_tag="IWO38_002931"
     CDS             <1..>452
                     /locus_tag="IWO38_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ0314925.1"
                     /translation="QRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIKIIRYFFIK
                     KLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKT
                     GFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLAL"
BASE COUNT          152 a           87 c           77 g          136 t
ORIGIN      
        1 caaagagcca cgtatagagc ggtttgttcc gtcttatttc ctaatggtga ctttcctagt
       61 aggagtcgat tcactcgctt aagttcaaac ttagcttaca ctatcaagat tattcgatac
      121 tttttcatca agaagctcac caaaggtgaa ctagtcggaa ttatagatag ttttcctagt
      181 ccattatgta aaccagttag aaatagacaa gcaaaactat tgaatcaaat agccaaagtt
      241 ggctataact caacaaaaaa atcttatttt tatggtctta aaattcatat gatcgttact
      301 aagactggct ttccgattac ttactcaatc acgaatccag gtgtccatga cgtgaaagtg
      361 ttagagactt tatcggaaga agcgaatctt cccaatatcc taggggacaa aggatatatt
      421 agccataaaa tccatgaaaa gcttgccctt aa
//
LOCUS       DACECY010000209          444 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.224, whole genome shotgun sequence.
ACCESSION   DACECY010000209 DACECY010000000
VERSION     DACECY010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.224"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          207 a           56 c           68 g          113 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caat
//
LOCUS       DACECY010000210          437 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.225, whole genome shotgun sequence.
ACCESSION   DACECY010000210 DACECY010000000
VERSION     DACECY010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.225"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            82..>437
                     /locus_tag="IWO38_002932"
     CDS             82..>437
                     /locus_tag="IWO38_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ0314926.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRY"
BASE COUNT          153 a           75 c           87 g          122 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataa
//
LOCUS       DACECY010000211          423 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.226, whole genome shotgun sequence.
ACCESSION   DACECY010000211 DACECY010000000
VERSION     DACECY010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.226"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            81..>423
                     /locus_tag="IWO38_002933"
     CDS             81..>423
                     /locus_tag="IWO38_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0314927.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSG"
BASE COUNT          166 a           77 c           76 g          104 t
ORIGIN      
        1 taaaagtaaa aaggccatat aacagtcctt ttacggtaca atgtttttaa cgacaaaaac
       61 atacccagga ggacttttac atgacccaag tacattttac actgaaaagc gaagagattc
      121 aaagcattat tgaatattct gtaaaggatg acgtttctaa aaatatttta acaacggtat
      181 ttaatcaact aatggaaaat caacgaacag aatatattca agcaaaagaa tatgaacgaa
      241 cagaaaaccg acaaagtcaa cgaaatggct attatgagcg cagctttacg acacgtgtag
      301 gcacgctaga attaaaagta cccagaacac gtgatggcca tttttcaccc acagtgtttg
      361 aacgttatca acgaaacgaa aaagccctca tggcttcaat gttggaaatg tatgtatcag
      421 gcg
//
LOCUS       DACECY010000212          415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.227, whole genome shotgun sequence.
ACCESSION   DACECY010000212 DACECY010000000
VERSION     DACECY010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.227"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>415
                     /locus_tag="IWO38_002934"
     CDS             <1..>415
                     /locus_tag="IWO38_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0314928.1"
                     /translation="KSVSKSFVSSLTEQLEPMVNEWQNRLLSEKNYPYLMTDVLYIKV
                     REENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEYLKERGLQGTELVI
                     SDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIP"
BASE COUNT          143 a           84 c           76 g          112 t
ORIGIN      
        1 taaatccgtc tctaagtcct tcgtttctag cttaacagaa cagctagaac ctatggttaa
       61 cgagtggcag aatcgtttat tatcagaaaa aaattatcct tacttaatga ccgatgtact
      121 ctatataaaa gtacgagaag aaaatcgagt actctcaaaa agctgtcata tagcgattgg
      181 aataaccaaa gatggcgacc gtgaaattat cggcttcatg attcaaagtg gcgaaagcga
      241 agagacctgg acaacatttt ttgaatacct aaaagaacgc ggtttacaag gtacggaact
      301 cgttatttct gatgcgcaca aaggattagt ctctgccatt agaaaatcct tcaccaacgt
      361 aagttggcaa agatgccaag ttcacttcct aagaaatatc tttaccacca ttcct
//
LOCUS       DACECY010000213          405 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.228, whole genome shotgun sequence.
ACCESSION   DACECY010000213 DACECY010000000
VERSION     DACECY010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.228"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..382
                     /locus_tag="IWO38_002935"
     CDS             <1..382
                     /locus_tag="IWO38_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0314929.1"
                     /translation="KEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIEN
                     QDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYN
                     QKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          157 a           72 c           63 g          113 t
ORIGIN      
        1 aaaagaaatc tgtgatgact ttaaggctgt ttatcaagct aactcaaaag aagaagcgaa
       61 taccttctta tccggcatga ttgagaaatg gaagaaaaac tatcctaaag tgacgcagtc
      121 actcatagaa aaccaagact tattaacttt ttatgatttt ccacctagca ttcgtagaac
      181 catttactca accaatctaa tcgagtcttt caataagcaa attaaaagat acagccgtag
      241 aaaagagcag tttcaaaatg aagaatcact agaacgcttt ctagtcagca tttttgatac
      301 atacaatcaa aaatttctaa acagaagcca taaaggtttt caacaggtaa ccgatacatt
      361 agtttcaatg tttactgagt aactaattat tttgcaggag gacaa
//
LOCUS       DACECY010000214          394 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.229, whole genome shotgun sequence.
ACCESSION   DACECY010000214 DACECY010000000
VERSION     DACECY010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.229"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..285
                     /locus_tag="IWO38_002936"
     CDS             <1..285
                     /locus_tag="IWO38_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0314930.1"
                     /translation="EVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHM
                     LQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          117 a           73 c           92 g          112 t
ORIGIN      
        1 gaggttgcag aagatgatcc ttattgggaa atcgaagcct cctatattgc tcaatgtgcc
       61 tacaatacaa ccttgatgtt ttctccagat gtgatcattt ttggcggtgg tgtgatgaaa
      121 caacgccata tgctgcaaaa cgtccacgac gcatttgcaa aattggtcaa tgggtatgtc
      181 gaaacacctg agttaagcaa atatatcgtg accccagcat tagaagacaa cgctggaacg
      241 ctcggttgtt tggcattggc tcgagaagcg gtcttacatt cataaacgaa aaaaggctct
      301 tcgacattta gtgttggtag agaaagatac aactgaaaat ttagtcataa ccagttaggc
      361 agagtgctta actggttttt gttttacttg gaag
//
LOCUS       DACECY010000215          394 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.230, whole genome shotgun sequence.
ACCESSION   DACECY010000215 DACECY010000000
VERSION     DACECY010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.230"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          135 a           63 c          100 g           96 t
ORIGIN      
        1 gtgaaaagct gtgggttgca agtagtctaa aaactattgt aataactctt ataacgagaa
       61 gtcaaatgag tgggtaacgc cacaaagggc ttccactgat actccgacat gcaacaagta
      121 gtttatgcag accaacgttg tgtgaagctc ggtgaagtcg gctgaaggat accgtaaggt
      181 aaaagaaata gtgaatacca cgaaagttct ttagaggtaa agggtgtatc cgataagtcg
      241 ggtgtccaaa aaaacttaat atggtgagta tgtacaaacg tactgacgaa ctagcgaatg
      301 tactgtccga aaggcatagc tcctagaaat gggagagaca gtgagcaaaa aatcactcat
      361 tgtgctaact tgtggttgag taaaagtcgt tgtt
//
LOCUS       DACECY010000216          379 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.231, whole genome shotgun sequence.
ACCESSION   DACECY010000216 DACECY010000000
VERSION     DACECY010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.231"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..350
                     /locus_tag="IWO38_002937"
     CDS             <1..350
                     /locus_tag="IWO38_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0314931.1"
                     /translation="EAVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFE
                     DAFQYTVQGNSHNRLKSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDE
                     WIYSSRKYINFDK"
BASE COUNT          141 a           66 c           66 g          106 t
ORIGIN      
        1 gagaagctgt taaaggaatt tttaagttca cagatattaa cttagcgcgt gaggctaaaa
       61 atcgattgat tcatgattat atcgatcaac caaaatattc aaaagcttgc gcatcattgg
      121 atgatggatt cgaagacgcc tttcaatata ccgtacaagg aaattcccac aatcgactaa
      181 agagtaccaa tctaattgaa cgactgaatc aagaagtacg cagaagagaa aagattattc
      241 gcatcttccc caatcaaaca tcagccaatc gcttaattgg agccgttctt atggacctac
      301 atgatgaatg gatttattct tcaagaaaat acatcaattt tgataagtag aaatggtaaa
      361 aacattgtat agcatttta
//
LOCUS       DACECY010000217          363 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.232, whole genome shotgun sequence.
ACCESSION   DACECY010000217 DACECY010000000
VERSION     DACECY010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.232"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          154 a           47 c           56 g          106 t
ORIGIN      
        1 agaactatat aaaacagcat gtttctcaaa ttatgagatg ttaagaaaaa tgattcgaga
       61 ggttttagaa ctatataaaa cagcatgttt ctcaaacaaa gtgcagaacg aaaatcaatt
      121 catcacagtt ttagaactat ataaaacagc atggttttaa aacaagaaat aagtcagttt
      181 tccttttgct ttagtaagga aaaatagagt attctcaata aaacttaaaa taagaaagaa
      241 taagcaaacc tttgttttaa tagctaatcg aagtaaaacc caaagccata tgaactcctg
      301 aatcagcgaa agtttatgat gattgggctt actaataatt taggaattag atggtaaatc
      361 taa
//
LOCUS       DACECY010000218          347 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.233, whole genome shotgun sequence.
ACCESSION   DACECY010000218 DACECY010000000
VERSION     DACECY010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.233"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            72..>347
                     /locus_tag="IWO38_002938"
     CDS             72..>347
                     /locus_tag="IWO38_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0314932.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
BASE COUNT          138 a           59 c           62 g           88 t
ORIGIN      
        1 aaataaaaag gcatttatgg tacactcaaa ttgtatttcc gacgaaagaa aacaaaggag
       61 tgtaaccata aatgacctac aaacatctta ccatagacga actgacaatg atagaatcat
      121 attatcttca acataataag ccggttgaaa tcgctaaccg aatgggacgt gctatacaaa
      181 ctatttataa tgtagtcaat aagttcaagc aaggcaagac tgctcttgat tattggcacc
      241 agtataaaga aaataagaaa aaatgtggta gaaaagtcat tcaattacct gctcatgaag
      301 tagattacat taaagagaaa gtcactcttg gttggacgcc tgacgtc
//
LOCUS       DACECY010000219          345 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.234, whole genome shotgun sequence.
ACCESSION   DACECY010000219 DACECY010000000
VERSION     DACECY010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.234"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          113 a           65 c           56 g          111 t
ORIGIN      
        1 atctgttcta acattctcaa ctttgtcagt ttaccatagg aaaatgggaa aatctacgaa
       61 agctctcaac agaatcgtcc tacttaaata cgcttttctt taataataaa taagttggtt
      121 gtttttgtta tttgcgcttt cacttttcgg aataaataat gtgtatttat atggaataaa
      181 taaaaaaaga ggtcgtgact aaaaacaagt cacaacctct ggtaaacggt gttctcctga
      241 caactcctcg gtcacaagac acaatattca acaaacatga gaagctattt tttgaaattg
      301 ctctttgtga ctcagagtga aacggcaggc tcacgacctc tttct
//
LOCUS       DACECY010000220          332 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.235, whole genome shotgun sequence.
ACCESSION   DACECY010000220 DACECY010000000
VERSION     DACECY010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.235"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>332
                     /locus_tag="IWO38_002939"
     CDS             <1..>332
                     /locus_tag="IWO38_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314933.1"
                     /translation="IILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAE
                     YTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQ
                     GLYGWNVT"
BASE COUNT          103 a           53 c           78 g           98 t
ORIGIN      
        1 attatacttt ctttcttagg gaaagtacaa atggaagtga ttagtgcact gttgcaagaa
       61 aagtatcatg tggagataga actaaaagag cctacagtca tttatatgga gagaccgtta
      121 aaaaatgcag aatataccat tcacatcgaa gtgccgccaa atcctttctg ggcttccatt
      181 ggtttatctg tatcaccgct tccgttggga agtggaatgc agtatgagag ctcggtttct
      241 cttggatact taaatcaatc atttcaaaat gcagttatgg aagggatacg ctatggttgc
      301 gaacaaggat tatatggttg gaatgtgacg ga
//
LOCUS       DACECY010000221          323 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.236, whole genome shotgun sequence.
ACCESSION   DACECY010000221 DACECY010000000
VERSION     DACECY010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.236"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..302)
                     /locus_tag="IWO38_002940"
     CDS             complement(<1..302)
                     /locus_tag="IWO38_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAQ0314934.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DACECY010000222          321 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.237, whole genome shotgun sequence.
ACCESSION   DACECY010000222 DACECY010000000
VERSION     DACECY010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.237"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>321)
                     /locus_tag="IWO38_002941"
     CDS             complement(<1..>321)
                     /locus_tag="IWO38_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0314935.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DACECY010000223          302 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.238, whole genome shotgun sequence.
ACCESSION   DACECY010000223 DACECY010000000
VERSION     DACECY010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.238"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(89..162)
                     /locus_tag="IWO38_002942"
     tRNA            complement(89..162)
                     /locus_tag="IWO38_002942"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(126..128),aa:Ile,seq:cat)
BASE COUNT           96 a           57 c           64 g           85 t
ORIGIN      
        1 tcatttgagt ggcaatgaag aaaagcttta gcttgtataa tactcttcaa aaaattctaa
       61 cacagaacga ttcaaatggc tacgttaatg taccctgtag gactcgaacc tacgaccgga
      121 cggttatgag ccgtctgctc tgaccaactg agctaagggt actggttgtt gccacataaa
      181 gccataaaca atcaaccagt agaatgtgtg gcaacaaacc tgttatcgca tagcttggag
      241 tgtgactatt atgggtgata gtgaagatat gcgataacat cactatttta tcgaatgatt
      301 tt
//
LOCUS       DACECY010000224          294 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.239, whole genome shotgun sequence.
ACCESSION   DACECY010000224 DACECY010000000
VERSION     DACECY010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.239"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            22..>294
                     /locus_tag="IWO38_002943"
     CDS             22..>294
                     /locus_tag="IWO38_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0314936.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPD"
BASE COUNT          111 a           55 c           57 g           71 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat catttttcct
      241 gctgaggaaa aagaatacat tgaaaaaaga tcaactgaag gttggacccc agac
//
LOCUS       DACECY010000225          283 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.denovo.240, whole genome shotgun sequence.
ACCESSION   DACECY010000225 DACECY010000000
VERSION     DACECY010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.denovo.240"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>283
                     /locus_tag="IWO38_002944"
     CDS             <1..>283
                     /locus_tag="IWO38_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314937.1"
                     /translation="SDADKYNPEGQKVTTELNKEPDASEGIKNKEDLPKDTKYTWKEK
                     VDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKV"
BASE COUNT          117 a           40 c           66 g           60 t
ORIGIN      
        1 atctgacgct gacaaatata atcctgaagg tcaaaaagtg actacagaat tgaataagga
       61 accagacgca tctgagggaa ttaagaacaa agaagattta ccaaaagata ctaagtatac
      121 ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa aaagggactg ttgtagtgac
      181 atatccagat ggatcatctg atgaagttga agtagatgtt acagtgacag acaatcgttc
      241 tgacgccgat aaatatgagc caacagtaga aggcgaaaaa gta
//
LOCUS       DACECY010000226        53406 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.guided.014, whole genome shotgun sequence.
ACCESSION   DACECY010000226 DACECY010000000
VERSION     DACECY010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.guided.014"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..817
                     /gene="rpoD"
                     /locus_tag="IWO38_002945"
     CDS             <1..817
                     /gene="rpoD"
                     /locus_tag="IWO38_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ0314938.1"
                     /translation="PVRMYLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLR
                     LVVSIAKRYVGRGMQFLDLIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAI
                     ADQARTIRIPVHMVETINKLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIA
                     QEPVSLETPIGEEDDSHLGDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVL
                     RLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            1037..3136
                     /locus_tag="IWO38_002946"
     CDS             1037..3136
                     /locus_tag="IWO38_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ0314939.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            3332..4195
                     /locus_tag="IWO38_002947"
     CDS             3332..4195
                     /locus_tag="IWO38_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0314940.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYGFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            4300..4773
                     /locus_tag="IWO38_002948"
     CDS             4300..4773
                     /locus_tag="IWO38_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0314941.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            4869..5756
                     /gene="xerD"
                     /locus_tag="IWO38_002949"
     CDS             4869..5756
                     /gene="xerD"
                     /locus_tag="IWO38_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ0314942.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            5775..6146
                     /locus_tag="IWO38_002950"
     CDS             5775..6146
                     /locus_tag="IWO38_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0314943.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            6116..6913
                     /locus_tag="IWO38_002951"
     CDS             6116..6913
                     /locus_tag="IWO38_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ0314944.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            6897..7466
                     /gene="scpB"
                     /locus_tag="IWO38_002952"
     CDS             6897..7466
                     /gene="scpB"
                     /locus_tag="IWO38_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ0314945.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            7472..8188
                     /locus_tag="IWO38_002953"
     CDS             7472..8188
                     /locus_tag="IWO38_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ0314946.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     regulatory      8270..8395
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            8518..9129
                     /locus_tag="IWO38_002954"
     CDS             8518..9129
                     /locus_tag="IWO38_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0314947.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     gene            9507..11030
                     /locus_tag="IWO38_002955"
     CDS             9507..11030
                     /locus_tag="IWO38_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ0314948.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            11267..12607
                     /locus_tag="IWO38_002956"
     CDS             11267..12607
                     /locus_tag="IWO38_002956"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ0314949.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            12760..13497
                     /locus_tag="IWO38_002957"
     CDS             12760..13497
                     /locus_tag="IWO38_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ0314950.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(13662..13883)
                     /locus_tag="IWO38_002958"
     CDS             complement(13662..13883)
                     /locus_tag="IWO38_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ0314951.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            13914..14945
                     /locus_tag="IWO38_002959"
     CDS             13914..14945
                     /locus_tag="IWO38_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314952.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            14932..16335
                     /locus_tag="IWO38_002960"
     CDS             14932..16335
                     /locus_tag="IWO38_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ0314953.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            16401..17036
                     /locus_tag="IWO38_002961"
     CDS             16401..17036
                     /locus_tag="IWO38_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0314954.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            17085..17765
                     /locus_tag="IWO38_002962"
     CDS             17085..17765
                     /locus_tag="IWO38_002962"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ0314955.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            17900..19129
                     /gene="rpsA"
                     /locus_tag="IWO38_002963"
     CDS             17900..19129
                     /gene="rpsA"
                     /locus_tag="IWO38_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ0314956.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            19311..20621
                     /locus_tag="IWO38_002964"
     CDS             19311..20621
                     /locus_tag="IWO38_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ0314957.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            20864..21139
                     /locus_tag="IWO38_002965"
     CDS             20864..21139
                     /locus_tag="IWO38_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ0314958.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            21374..22420
                     /locus_tag="IWO38_002966"
     CDS             21374..22420
                     /locus_tag="IWO38_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314959.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            22422..23681
                     /locus_tag="IWO38_002967"
     CDS             22422..23681
                     /locus_tag="IWO38_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ0314960.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            23687..24268
                     /locus_tag="IWO38_002968"
     CDS             23687..24268
                     /locus_tag="IWO38_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ0314961.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(24350..25225)
                     /locus_tag="IWO38_002969"
     CDS             complement(24350..25225)
                     /locus_tag="IWO38_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0314962.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            25373..25705
                     /locus_tag="IWO38_002970"
     CDS             25373..25705
                     /locus_tag="IWO38_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ0314963.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            25817..27025
                     /locus_tag="IWO38_002971"
     CDS             25817..27025
                     /locus_tag="IWO38_002971"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ0314964.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            27179..27634
                     /locus_tag="IWO38_002972"
     CDS             27179..27634
                     /locus_tag="IWO38_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314965.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            27996..28271
                     /locus_tag="IWO38_002973"
     CDS             27996..28271
                     /locus_tag="IWO38_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0314966.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            28284..30362
                     /locus_tag="IWO38_002974"
     CDS             28284..30362
                     /locus_tag="IWO38_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0314967.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            30522..32408
                     /locus_tag="IWO38_002975"
     CDS             30522..32408
                     /locus_tag="IWO38_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0314968.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            32420..33367
                     /locus_tag="IWO38_002976"
     CDS             32420..33367
                     /locus_tag="IWO38_002976"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0314969.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            33387..33914
                     /locus_tag="IWO38_002977"
     CDS             33387..33914
                     /locus_tag="IWO38_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0314970.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(33977..34630)
                     /locus_tag="IWO38_002978"
     CDS             complement(33977..34630)
                     /locus_tag="IWO38_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ0314971.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            34762..35604
                     /locus_tag="IWO38_002979"
     CDS             34762..35604
                     /locus_tag="IWO38_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0314972.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            35762..36658
                     /locus_tag="IWO38_002980"
     CDS             35762..36658
                     /locus_tag="IWO38_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0314973.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            36661..37374
                     /locus_tag="IWO38_002981"
     CDS             36661..37374
                     /locus_tag="IWO38_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ0314974.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            37394..37912
                     /gene="msrA"
                     /locus_tag="IWO38_002982"
     CDS             37394..37912
                     /gene="msrA"
                     /locus_tag="IWO38_002982"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0314975.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            37909..38136
                     /locus_tag="IWO38_002983"
     CDS             37909..38136
                     /locus_tag="IWO38_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ0314976.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            38288..38839
                     /gene="lepB"
                     /locus_tag="IWO38_002984"
     CDS             38288..38839
                     /gene="lepB"
                     /locus_tag="IWO38_002984"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0314977.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(38922..39464)
                     /locus_tag="IWO38_002985"
     CDS             complement(38922..39464)
                     /locus_tag="IWO38_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0314978.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(39701..40099)
                     /locus_tag="IWO38_002986"
     CDS             complement(39701..40099)
                     /locus_tag="IWO38_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0314979.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            40359..41219
                     /gene="ylqF"
                     /locus_tag="IWO38_002987"
     CDS             40359..41219
                     /gene="ylqF"
                     /locus_tag="IWO38_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ0314980.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            41212..41979
                     /locus_tag="IWO38_002988"
     CDS             41212..41979
                     /locus_tag="IWO38_002988"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ0314981.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            42035..42892
                     /gene="dprA"
                     /locus_tag="IWO38_002989"
     CDS             42035..42892
                     /gene="dprA"
                     /locus_tag="IWO38_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ0314982.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            43014..45092
                     /gene="topA"
                     /locus_tag="IWO38_002990"
     CDS             43014..45092
                     /gene="topA"
                     /locus_tag="IWO38_002990"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ0314983.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            45061..46437
                     /gene="trmFO"
                     /locus_tag="IWO38_002991"
     CDS             45061..46437
                     /gene="trmFO"
                     /locus_tag="IWO38_002991"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ0314984.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            46635..47540
                     /gene="xerC"
                     /locus_tag="IWO38_002992"
     CDS             46635..47540
                     /gene="xerC"
                     /locus_tag="IWO38_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ0314985.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            47577..48125
                     /gene="hslV"
                     /locus_tag="IWO38_002993"
     CDS             47577..48125
                     /gene="hslV"
                     /locus_tag="IWO38_002993"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ0314986.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            48139..49539
                     /gene="hslU"
                     /locus_tag="IWO38_002994"
     CDS             48139..49539
                     /gene="hslU"
                     /locus_tag="IWO38_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ0314987.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            49557..50348
                     /gene="codY"
                     /locus_tag="IWO38_002995"
     CDS             49557..50348
                     /gene="codY"
                     /locus_tag="IWO38_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ0314988.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            50430..51305
                     /locus_tag="IWO38_002996"
     CDS             50430..51305
                     /locus_tag="IWO38_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ0314989.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(51437..52075)
                     /gene="plsY"
                     /locus_tag="IWO38_002997"
     CDS             complement(51437..52075)
                     /gene="plsY"
                     /locus_tag="IWO38_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ0314990.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            52202..52636
                     /locus_tag="IWO38_002998"
     CDS             52202..52636
                     /locus_tag="IWO38_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ0314991.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            52802..>53406
                     /locus_tag="IWO38_002999"
     CDS             52802..>53406
                     /locus_tag="IWO38_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ0314992.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLL"
BASE COUNT        18482 a         9122 c        11313 g        14489 t
ORIGIN      
        1 cccggttcgt atgtatctga aagaaattgg ccgtgtctcc cttttaactg cggaagaaga
       61 agtagcgtta gcattgaaaa tcgaagaagg cgaccaagaa gcgaaacagc gtctggcaga
      121 agcaaacctt cgtttagtag taagtatcgc taaacgttat gtaggacgag gcatgcagtt
      181 ccttgacttg atccaagaag gaaacatggg actgatgaag gcagttgaaa aatttgacta
      241 ccgcaaagga tttaagtttt caacctatgc tacatggtgg attcgtcaag ccatcactcg
      301 agcaatcgct gaccaagcac gtacgatccg tattccagtt catatggttg aaacaattaa
      361 taaattgata cgtatccaac gtcaattgct gcaagacctt ggaagagaac ctacgccaga
      421 agaaatcggt gcggaaatgg atcttccaac agaaaaagtc cgagaaatct tgaaaatcgc
      481 gcaagaacct gtttcccttg aaacgccaat cggtgaagaa gatgactctc acttaggtga
      541 tttcatagaa gaccaggaag caacaagtcc agcagaacac gcagcttatg agttactaaa
      601 agaacaattg gaagatgtac tagatacatt gactgatcgg gaagagaatg tccttcgctt
      661 acgttttggt ttagatgatg gccggacgag aacattggaa gaagtaggga aagtgtttgg
      721 tgttactcgg gaacgtattc gtcagatcga ggctaaagca ttaagaaaat tgcgccatcc
      781 ttctcgttca aaacaattaa aagatttctt agaataagcg tggaccattg accagaagag
      841 aaatcagtaa aactgtttgg tttattttaa aagaaaaaaa gcttgagact acgcagtctc
      901 aagctttttt ttctatagga aggaaattca ttcattattt ttaagtaaat cctaaaaata
      961 agctattggc gtgacgtata gaatatgata gattaaatgg tagcgtaaac ctagtaaagg
     1021 agccgatcgc taagtgatga aaaaatgtcc aaattgcgga tcgaaaaaaa tggagcagaa
     1081 tactatctgt ccagactgcg gtcatcaaat aactgcagaa gatcagcaaa taaaagaatc
     1141 aattgaagaa acaaatacag atgcagctat gcaggataaa tcaactgaag atgcagtctt
     1201 tcctgataca gaattaaacg atccaatcga atggtcggaa ttgaaagatt tacctctaga
     1261 atctgtaatg gaattattcg aaaaagcaga accagaagac gaagaaccac ctgctcacga
     1321 tcaagtaact aatatagaga aaaatgaaca gaagaaagca actgttcatg agacttcgga
     1381 agaatcagga aagaatcaag aagaaacgct tgaatcaaaa caaaaaagaa gagtagctga
     1441 actgaaagaa atcgtcgata atgaggaaga aaattcgata ctatccgctt atatcaaagc
     1501 acatcgtgaa gatacaaaag aagagcacgc gaaagagctg ctcaaaatga tcagtgaaaa
     1561 aattgcacag gaaaacaaag tagagccaga aaaggaacaa gaaactgaaa aagactctga
     1621 tagagaaaac gccacaggta aagaaacagt aaaaataaaa gacgtaaaaa cggatatcac
     1681 agaaaaaact gaagtattta ctgaaaaaca attagaaaag gaaaaaacga ataaactgcc
     1741 ggaagcagag aaaaaagaaa tactcgaaaa cacagacaaa aagtcaacag agcccgatga
     1801 aataaatagt gaatccgatg ctgaaaatca atctgtaagc aaagagggta ctgatcttga
     1861 agcaaaaata gaaaaagaag agaaccagcc taagctgttt gaagcagaag cgaaatcaat
     1921 aacaatcgtg ccagatactg aaaaaacaca tcaagaatca aacttagaaa aaacagatcc
     1981 agataaagcg caaaagaaaa aatcaaaaaa agtaccttat ctcgttttag cagcagtact
     2041 tctactcggt ggaggaagct gggcttatta tgaccatcat caaaaagtag aagccgaagt
     2101 agcagcagaa gcaaaaagac agcaacaaat ggaagaactt catgagaaac ttgctgcctt
     2161 ttatacagat gatcaccatc aatttattcg tgcagatatg attcatcaag atttatcaaa
     2221 actaaaaaat gatttatcca gtcttaagaa tgaaaaagga tattcagagt tagaaacaac
     2281 ttaccaagat atccaatcta agatccgaga tatccaaaaa gtgaatgagt ggttcatagc
     2341 acctgccatc gtcgatgatc atctagcaga tgacatcaag ttgaaagcag ataaagagat
     2401 ccaagcaatc gaaacggata acacagattt tggaaaattg attcaacaag cacaaacaga
     2461 agctaaaaat caatatgctg aattgcaaac agctaaagaa aaaacagcta tagtctttaa
     2521 agacggaaaa gtagtggatt cagcaacacg cgaccaatac aagacggcaa aagaagcagt
     2581 agaaaaagtg aaaaatacag atctatctgc tcagttgaat gaacaattga aacaagtaga
     2641 taagatgctc actgataaag agaaaaaaga agaagaggaa aagaaaaaag cagaagaagc
     2701 gaaaaaagca gctgaagaac aagcaaaaca agccgctcaa gcagcagctg cagctcaagc
     2761 cgctcaacaa gctgcggtcg cacaagcagc acagcaagaa gcggcatcct cgaccccttc
     2821 ctcttcaaac tcagcgaatc agccaatcaa atctgccaga gcaagtgata ttgccgatgc
     2881 atctaatcca gcatggacgt gggcaccagg tgtccaaagc aaagtgattg ctacatgtat
     2941 ccaacgtgga tatatcgttg aaggaggtta cagattagaa aaagccagaa tcgaaaatgg
     3001 ggaaggctat tataatctat atgccacttc aacaaaatct gcattgatga aaggcattgg
     3061 agaaagtgct ttgccatttt acatcgtaac catcaactgt aaaactggat ggttcggagg
     3121 aaatgggagc aattaatttt aggcaaatga aagaggctgt gagcctgccg tttaacaccg
     3181 tttattcgaa aaaggggagt atgacacaac tgttgtcata ctcctttttt cacctttcca
     3241 aaccaaggct cagctattca agtagataac attatgttat gatagcaaag aaatgtatta
     3301 agaaaacctt tcactataga gggagaaaaa aatgaacgat ttattagcaa ctatatttac
     3361 aggtattatt attgatgaaa atgaaaaaaa ctactttgtc caaaaaaacg gacaaacctt
     3421 tggcttaagc aaatcagaag gtgaacacaa aatcggtgaa gcagttgaag gattcggtta
     3481 ccaaaatcaa aaacatgaaa atcggatcac tacacaaatt cctaaaagcc gtatcggtca
     3541 ttacggtttt ggaatcgtta ctgctacacg aaaagattta ggtgcgtttg tcgatatcgg
     3601 tcttccagat aaagactttg tcgtatctct tgatgaattg ccgacaatga cagagctatg
     3661 gcctaaaaaa ggggatcgct tgatgatttc ccttagagtt gatgcaaaag accgtatttg
     3721 gggcagttta gcagatgaaa aaatattcaa atctttaagc aaatatggaa cagaagaaat
     3781 gaaaaacaaa gatgtgatag gaacagcata ccgcttgaaa ttaacaggta cgtacgtttt
     3841 aacggaagat ttctatcttg ggtttatcca tccttctgaa cgatataatg aacctcgtct
     3901 aggcgagcag ataaaaggtc gagtgattgg tgtccgacca gacggtgtac tgaatctttc
     3961 attgaagcca agagcatatg aagctatatc agatgatgca caaatgattt taaccatttt
     4021 agaacgttct gcagatcagc aaatcaactt tacagataaa tcggatccag aagaaatcat
     4081 gaaagctttt ggtatcagca aaggagcatt taagcgcgca ttaggtaatc ttttgaaaca
     4141 aggattgatt acacaagaaa acggtgtgac aaaactagtc aaaaaatcta attgagaatc
     4201 ccttgcatgt ttttcttaac tagcttataa taattttaat taacatagtg ttatatcttt
     4261 ttttatttgt aattattata aattgttggg gttgagtaaa tggtcatgaa tgctatttcg
     4321 gcgatgaaaa aaacaaaaca gcagctacat gaagcggggt ttaagctgac accgcaaaga
     4381 gaagctactc tgcttgtgct tttggaaaat gagaaagagc atttatctgc agaagaaatc
     4441 ttctttttag tgaaaaaaaa gagtccggaa attggacttg ctactgttta tcgaaccctt
     4501 gaaatcttga cagaattgaa aattgtcgat aaagtaagtt ttaatgatgg attagcccgt
     4561 tatgatttga gaaaagaagg cgccaaacat ttccatcatc atttactatg cttgaactgc
     4621 ggcacgatcg aggaagtaga agaggatctt ttacttgaag tggagcaagt agtagaagaa
     4681 cgttatcatt ttttagtaaa agatcatcgg ctaacgttcc atggaatctg tcaggaatgc
     4741 tatgaaaaag aaaaaagaaa ccagtccgtc tgagtgtcta gacggctttt tctttgctca
     4801 gattatggaa aaagactatc agaatgaagc ggtatctgat atgatagaga ggcatgaggg
     4861 ggattattat gaaagagcaa atcaacgatt atctccacta cttgaatatt gaacggggat
     4921 tgtccgccaa tacacgacaa agctatgaac gtgatttgga acagtacttg cattttctac
     4981 aggagcagaa aattgcttca tggcaggatg tcgatcgctt tttagtcatc cgttttctgg
     5041 agaagttgca tgaagaaaaa aaagcgacag caactgttac tcggatgatc tcaagtctca
     5101 gaagattcca tcagttttta cggcaagagc gtatcactga tcatgatccg atgcaacata
     5161 tcgatacgcc gaagaaagca cagaaattac caagtacatt gactctgaca gaagtagaaa
     5221 agctgattga agcacctgat acgacaaaaa cgttagggat aagagatcgt gcaatcttgg
     5281 aagtcatgta tgctactggg atgcgcgtca gtgaattgat tggattgaaa ttgggagatc
     5341 ttcatctttc tcttggactg gtacagacag ttggaaaagg agataaagag cggatcattc
     5401 ctttaggtga ttatgcgatc caatggctgg aacgctactt agacgaagca cgtccattac
     5461 ttgtaaagga cgcatctgaa atgcatgtat ttgtcaataa tcatgggaaa ggtctttctc
     5521 ggcaaggaat ctggaaaaat ctgaagcagt tagtacgaga ggctgggatt tacaaagaag
     5581 taacaccaca tacgctgcgg catagctttg ccacacatct gctagaaaat ggagctgatt
     5641 tgcggacagt acaggaattg ttaggtcacg cggatatctc tacgactcag atttatacac
     5701 atatcacgaa gaaaagaatg acggatgttt ataagcagca ttttccaagg gcttagtcat
     5761 aggaaggtga gaagatgtta acacattacc ggaaagaaaa ccgtaaaatt gctatgggac
     5821 tattgagttt ccataagaaa ctgacaggta aaacttcttt gttgaaggag attgatacgt
     5881 acgaatctga agacgattat gaactttttt tctttacgcc agaaggttca accaacattc
     5941 aagggattat tggtatttac tgggcaacga acgatcagct gatcatccat gacatttctt
     6001 tgaatccgtc ttatcgcggt gagaatatgg gctttcaaat gttagatgaa ttacaggaaa
     6061 agtacccgga tgtctccatc cgtgcaactg aaatcacaga gccttacatt ggaaaatggc
     6121 tagcaaagaa aaagaagaaa gagtgaatga gttgacggag atcaatatta aattagatgt
     6181 attcgagggt cctctcgatc tgctgcttca tctgattcag caaatggaaa tcgatatata
     6241 tgatataccg atcgctacga tcacggagca atatatgaat tatatccata caatgaaaac
     6301 actcgagcta gctgttgcag gagaatactt agtgatggct gctacattga tggcaatcaa
     6361 aagcaagaca ttgcttcctg tccaagaatt cgtcacagac gatgaagaat tatgggaaga
     6421 agatcctcga gaagaattgg tcactcagct tttagaatac cgtaaatata aatatgcagc
     6481 tgaaagactg acagaaaaag cagaggaacg aagtctttat tacacaaaag aacctatgaa
     6541 catcgatggg ttagcggatg ctgaaaagcc gttgaagcga ggacaagtca aaaaaatcga
     6601 tttgatccgt gccatgcaga aaatcttaga acgtaagaaa ttttctcaaa aagtggaaaa
     6661 aacgatcacg cccgatgaca caacgatcga agaacgaatg gtatttatcg aagagcgtat
     6721 aaaaagaaat gcgcatggct gtactttcga ttcttttttt gagtcacctt ctaaaagtga
     6781 agttgtcact acttttatgg ctttgctaga attgatgaaa aaagggcaga tcattgcgca
     6841 acaaaaagaa aactatgaat caattatgct ttatccagca gtaggagata ccgaacatga
     6901 cgaaattaag tgaacttgaa gcccttcttt ttattgttgg cgaggaaggg atcagtaatg
     6961 aagaattaag cggactgtta aatctcagcg aagcagaagt cacaagttta gtgaaagaat
     7021 taaaagaatc ttacactcaa aactcgcact cagctttgca tatttttgaa acggaaagcc
     7081 attatgtatt aacgactaaa aaagagctgg ctcccttact taagcgatat gcgcaaggga
     7141 catcaaacac attatctcaa gccgccgtcg aaaccttagc aatcatcgct tataaacagc
     7201 caatcacaag agctgaaatc gagcagattc gaggagttca ggtttcagga gcggtacagc
     7261 gtctagctgc ccataaactg attgaagaaa aaggtagagt agaaggacca ggtcggccga
     7321 tattatatgg aacaactggc tacttcttag attattttgg tttgaaatca ttagaagaac
     7381 tgcctgatac acaagaaatg gaaatttcac ttgaagaaga cgtgtcacca gatctattct
     7441 ttgatggaat gaataaagga gaataattgt aatggagaga ttgcagaaag taattgccca
     7501 cgctggcatc acttctcgtc gaaaagcaga agaattcatc cttgcaggac gtgtaaaagt
     7561 aaacggacaa gttgtaaggg aattagggat aaaagttggg aaacatgaca ctgtggaagt
     7621 ggatgaagtc ccaatttatc aagaagaata cggttactat ttattgtaca aacctaaagg
     7681 agttatttct gctgttgcag atgacaaagg aagaaaagtt gttacggatc ttttgccgat
     7741 ggtaaaagaa cgtatctatc ctgtaggtcg actggattac gatacttcgg ggatcttgct
     7801 actgacaaat gatggagatt ttgctcagcg cctgacacat ccaaaacatg aagtggataa
     7861 agtttacgtt gcaaaggtaa aagggatcgc tacaaaatcg atgcttgcac ctttgacaaa
     7921 aggaataaag atcgaaggac gaaaaacatc tccagctaaa ttccaaattt tatcagtgga
     7981 tcctaagaca aaccacagta tcgtggaatt gacgatccac gaaggacgta atcatcaagt
     8041 gaaaaatatg cttcaagcag ttggtcttcc tgtgcaaaaa ctaaaacgtg aaaaatacgg
     8101 agatctgact ttacaaggcc tacgtcctgg ggattaccgt gaactgaaca aaaaagaagt
     8161 cagcagtttg ctgaaccact caaaatgaac acttactttt tataagtgtt agatgttgcg
     8221 ttttttgaat agatatgtat aatgaatatg taaacaaaaa ttatataagg ttcgtcttca
     8281 ggggcagggt gaaattcccg accggtggtt atagtccacg acctattttg caaaaaaata
     8341 gctgaatcgg tgaaattccg ataccgacag tatagtctgg ataaagaaga tagagcttat
     8401 ttgacgacgc ttgatcaaat aactctaact ctattttccc tggaaaatag agttttttca
     8461 tttggagaga aacgtcaaaa agttcgctga agaggagtcc taacaggagg atttcaaatg
     8521 aagaacaccc gtgtacagaa aatggttgca gtagcgatgt ttgctgcgat cgcattagtt
     8581 ttacagtatt ttgcttttcc gatcatgccg gcttttagtt ttttgaagat cgattttagt
     8641 gatatctcgg tattgatcag catgttctta ttcggtccag cggccggagt tgcaacagcg
     8701 ttcttgcgtt cagtattaca tctgctgact acaggttttt ctccggataa tttggtagga
     8761 gacactgcta gtttcttatc aacaacgatc tttacattcc cgatttatta tttctttagt
     8821 gtaaagaaac agcataaagg aagaaacaaa tttttaggtg tcattacagg aaccatcgct
     8881 ctaactattt ttatgagtat cgcaaattat ttcgttatta cgcctcttta cttaatgttt
     8941 ttagggctga acgcaacaca gatgctgggc atgtctttag caaactatgt actgattggg
     9001 atcgttcctt tcaacttgat caaaggattc atcgtgagtg ctgtcttttt agtgctgcat
     9061 gcgcaacttc ttccatggtt aagtcgaaaa caacatcagt tcgaacaacg tcatacagca
     9121 gtcaaataaa tatcaaaaag atcaagaaat ttcttggtct ttttttttgg aaaaaaatac
     9181 aagacatgca ataatgtaaa cgttttattt gtttgttttg agtatttttt tgatttatgc
     9241 ataaaaatca atcaattctt tgcaaatgtg aaattcttct ccttttaaaa gacgaaaatg
     9301 tttgtttata aaaaaaagga aacgctacaa tgaaaaagaa gaaagcttta aaaacctgaa
     9361 aaaagaggag ggaataggga aaaacgaact ttttgaacaa aagttttaca aaatgcgtgt
     9421 tattattttt gttctgatac tctttttgat aaaataacat tagagccaaa attgttgtct
     9481 ctaacggaaa agaaaggaag aagaagatgg aacaaacaaa aaagaaaaaa tttcaaatgc
     9541 cttcagcgta tacggtctta tttattatta ttgcagtaat cgctgtactt acttggttta
     9601 taccagctgg gcactatagt gtagacgatg caggcaacat cgtcgctgga tcatatgaac
     9661 gagtacaaca aaatcctcaa gggctgtggg atattttcat ggcgccaatc aatgggatgc
     9721 taggtgtacc agcgggggag ctaactaaag aaacacctgc tgcaatccca atctctttct
     9781 tcattttggt tgttggtgga tttttaggta ttatcaacaa aacaggggca ctagatgccg
     9841 ggattgcttc agttgtgaag aaattcaaag gaaaagaaaa aatgcttatt ccagtattga
     9901 tgctgttatt tgcattaggc ggctctactt acggaatggc agaagaaacg attccattct
     9961 atgctttact gatcccagtc atgatggctg taggctttga tacagtagta gctgtagcaa
    10021 tcgtattagt agggtctcaa gtaggctgtc tagcttcaac ggtaaatcct tttgcaacag
    10081 gcgtagcttc acaagctgtt gggatttcga tgggtgaagg tattggtctt cgattcttga
    10141 tgttcgttat tttgttagct atttctattg tatatgtgta tcgttatgca tcaaagatcg
    10201 aaaaagatcc aacaaaatca ttagtataca accaacgtaa agaagatatg aagcatttcg
    10261 atattgaagc aatcggtcgt caagaagtaa tcacgaaaaa acaaaaacat gttaatgtat
    10321 tgttctttat tacttttggt atcatgatca tcagtttgat tccttggaca accttgaatg
    10381 cgaatttcac gatttttgaa tcattgacaa actggttaac aaaccttccg gtactaggta
    10441 cggttctagg taaaagcatg cttccattag gtgactggta cttcccagaa attactatgt
    10501 tattcttagt aatgtccatc gtagtagcaa tcacttatgg catgaaagag tctgaatttg
    10561 tctctgaatt tttagctggt gcttctgatt tgatcggtgt agcaatcgta gtagctgttg
    10621 ctcgtggtat ccaagtggtt atgaacaatg gattaatcac agacaccatc ttacattggg
    10681 gcgaacaagg attaagttca ttatcttcaa gtgtattcat cattattaca tttattttct
    10741 atatccctat gtcattcttg attccttcaa cttccggctt agctgctgca acaatgggaa
    10801 tcatgggacc tatgggtgaa tttgccggcg tcggtaagga tttagtaatc actgcttatc
    10861 aatctgcatc tggtttagta aacttaatta caccaacatc tgctatcgta atgggtgctg
    10921 tagcaattgc tcgtttcgat gtttcagtgt ggtggaaatt taccggtaaa ttaattgcga
    10981 tattatttgt agccatctgt gtgatcctag gtgtagcagc aatgttctaa acctagacaa
    11041 atacaggtac aaggtctatc ataagaatga agaaaccgat aaacggctat cgataagcac
    11101 gtggataagt caatagctcc attcagtgag tgttttttct ttcatcattt gttaagaaca
    11161 ttctgcttgc tgatggagca ggcttaaaac gtcattattt gagaagaaga ggttgtgtcg
    11221 gaggaatttg acatgaccct ttttaacata ttaggaggat aatatcatga aaaaatttgt
    11281 gacagaacaa catcatgaac aagcattaga atcattgagc gaattgatcc gaattccttc
    11341 cgtactagat gaagcagatt caggacaagg acatcccttc ggtaaaaaag tgattgaagc
    11401 tttggataaa gtattggaaa tcagtgaaaa gctaggattt agaacattta aagatccaga
    11461 aggctattac ggttattcag agattggctc tggagatgaa ttattcggta tcttgtgtca
    11521 tatggacgtt gttccagcag gggacgaaaa taattgggaa acaaaaccat tcgatccaac
    11581 tataaaagac ggctggttag ttggtcgcgg atcccaagat gacaaaggtc cttctattgc
    11641 ggcaatgtat gccgtcaaag ctttgattga tgctggcgtg gaattcaaga cacgtattcg
    11701 ttttatcttt ggtacagatg aagaaaattt atggcgttgt ttagaaaaat ataacgaaaa
    11761 agaagaagga attacgcaag gatttgcgcc agatgctgaa tttcctttaa tttatgcaga
    11821 aaaaggctta ttgcaagctt acttgacagg accaggaacg aacgagtttt ctgtaaaagc
    11881 tggaggagcg ttgaatgttg ttcctgatgc agctccttac tctggagaaa agctttctga
    11941 agtaaaagaa gcattgaaaa aacacgaatt cgatttcgaa gaacaaggtg aaggcatcgt
    12001 cgttcttggt aaaagtatcc acgcgaaaga tgcagcacaa ggagtgaatg ctatttctcg
    12061 tctggcaatt gctctttcag aagtatttga ttttggccca atcaatttct taggaaaatt
    12121 agttcaagaa aatgcaactg gtgaagctgt cgtgggcaaa acagaagatg aacaatccgg
    12181 cgagctaacg atgaactttg caagtttgga aatcacacca gaacaaacaa aaatcggtgt
    12241 agatatgaga atccctgtta cattcaagaa agatgattta gtagctaaat taactaaaac
    12301 agcagaaaaa tatgggttga cttatgaaga atttgacttc cttgattcat tatacgtgcc
    12361 actagacagt gagttagtga aaaatctatt ggggacatat cgcgatatta ctggagacat
    12421 gactgaaccg tttgtatcag gcggcgctac ttttgctcgt acgatgaacc agtgtgtggc
    12481 attcggtgcg atgttccctg atactcctga ctttatgcac caggcgaatg aacgatggga
    12541 attaagtagt atgtataaag caatggaaat ttatgcagaa gctgtgtatc gtttatgtgc
    12601 taaataaaaa taaacataca aaaagggcgg atattcaaat ggatattccg tcttttttta
    12661 tttgatgctg gaaagtttgc atttctctct tattttttta aggtataatc atacttgatt
    12721 aagaaaaagg ggtcactcag atagaaaagg tgggagaaag tgaaaatatt tagacgaata
    12781 ctaattggct tagtcatcat tttaggatta ctcggtggag ccggttatta ctatatacag
    12841 caaaatacgt acgaaccatc gtcagaagcg caagtgttgg taaaagaagg aaaaaacaaa
    12901 gaagattact tattttttga aggagagaaa aagaatcatg gaaagtctcc attacttatt
    12961 ttttatcctg gtgctctcgt agagccagaa agttatagta tttgggcgca agatttagca
    13021 aaaaacggct tttcagtcgc tattgtcaaa atgccacttg atctagcagt gatgggcgga
    13081 aacaaagcgg ataaggtcaa agaagatttt ccaaattccg attatgtgat cggtggccat
    13141 tcattaggtg gcgtaatggc aagtcgttat gtcaataaga atcattcaga caaacattta
    13201 aaaggggtat tcttcatggc aagttatccc gacaaaaaag gaacattgac agaatcagct
    13261 gttcctgttt tatcgatcac ggcgacaaaa gataaagtat tgaatcagga agcttatcaa
    13321 gaagctaagc agtatttacc ggaaagaaca gagtatgtat caatcaatgg cggaaatcat
    13381 ggaggttttg gcagctatgg tgagcaaaaa ggagatggag aggcagaaat ttctaacgca
    13441 gaccagcaaa ctgtgatcgt caatacattg gaaaactggc tgaaaaagtt gaaataatat
    13501 aaatgggtcc tatagggtcc gtgaaaaaac tagtcaatta actatagata gcaaatgaat
    13561 ctttcaaaat aagtcgggat ttcacggaac aagagaaaca gatcccggct tattttcaag
    13621 tttagagaac ttagtgtacg tttttaaagt ttcgtttata tttatatttt attttctaat
    13681 aaaatcgctc gaactggaca tttttgatac gcttttttta catatgtttc ttcttccggc
    13741 atcacgaact gttgatgagc ctctggttct tgggcaaata aaacgattcc ttcttcatca
    13801 taatcgaaaa tatctggcgc ttcgatttga caaagaccgc atgcaataca gcgttcaggt
    13861 acaagtttac atagtagtga catttctaac catcctaact taaggagaat aatatgattg
    13921 tttttatttt atcgctattt tcatcaggac acaagctgcg gattagttct ctttaccaac
    13981 tccttgtggg aaaacgaacg acttcagtac ttatttatgg atttacccac gaacttttgt
    14041 ttatccataa cagttttcca gaattgaagc aggataagtt ttatcaaatt ttgcaaaaaa
    14101 ttgcgcagca aggttggatc gaaataaatg aaaatgaagc aaagctaacg cctgcagggg
    14161 ctgatatgtt gtccgaacat caaatagaac atactggttt gcgttttgac cgctatggca
    14221 gaacagggga aactagttgg cggctcatta aatttgctgt tcaagtaatt tctaatctgg
    14281 caacgggtat tcaagactat cttccagcag agacaagtcc tttttatact ttccaactaa
    14341 aaaaatggct tagtgaaagc caattgccac gtgaaatttt gattgatact gcgtatgaga
    14401 gtttggtgca gctgttttcg gagataccag aagaagcagc agatttttta gctaaccaat
    14461 tatctggaaa tgagcgaaca gggctgcttc cttatcagct ggcaaaaaac aatgatgagt
    14521 cagcggttta tctgcaacaa agcagatgta tccacctttt gttagcacaa atcgaaaaac
    14581 ggccagattc gctttggcac gctttgatcg attcattatt acagcaaaat ttcaatcaaa
    14641 gcatgatgat caccaagcag atgtttatga atggtcagac aattgaccag atcatggcaa
    14701 tccgtcattt aaaaagagga acagtaacag atcatcttat tgagtgggcc ttgttttttg
    14761 atgattttcc atacgagtgg attctctctg cagaaacaat cgaacgtttg gaaccaaata
    14821 aggattccgt gcgagagtgg cgctttagcg aatggaatgt agatggacaa ttagattatg
    14881 gagaatttcg tttgtatcaa atctatctat tgagaaagga agcgatacag aatgttaata
    14941 aatgaattaa aaaaatattt tggatttgat tcatttcgtc ccggccaaga agaaattatt
    15001 caggcgttgc ttgatggaga agatactctc gctattttgc ctacaggaac gggaaaaagc
    15061 ctttgctatc aattgacggg gtatttaatg gaaggtctgg tcatcatcgt gtcaccgctg
    15121 ctttctctaa tggaagatca agtgacgcaa ttacaaaaac gaggggagaa acgagtagct
    15181 gctttcaata gtttactttc gcaatctgaa agaagatacg tcctccgtca tctatcgcaa
    15241 tataaatttt tgtttttgag tcccgaaatg ttgactaatc cttctctttt agagcatctg
    15301 aaaaaacaaa atattgcgtt atatgtagta gatgaggctc attgcgtgtc tcaatgggga
    15361 gttgatttcc gtccagaata tcaacagtta ggaaaaatcc gaaagctttt aggaaacccg
    15421 gtcacactgg cactaacggc gacagcaaca gatttagtag caagagatat acgtcatgta
    15481 ctatttgatc gaccaccaaa agagatcaga caatcagtca atcggcaaaa tatctcgctt
    15541 tttgttcgga aaacgcagca aaaagaacaa gaacttgaac acttcatgga aagagcacaa
    15601 ggagcggcaa tcatttattg tgcaacaaaa aaagaagtcg aacgattgta tcatttattc
    15661 agggaacgtt ttacagttgg atactatcac ggtggtcttg atgcagctca aagaagacaa
    15721 cttcaacagc agtttgtaaa aaatcagctg cagtttctca ttgctacgaa tgcatttggt
    15781 atggggatcg ataaatctga tatccgttat gtggtccatt atgatttgcc agacagtcta
    15841 gaaaactatg tacaagaaat cggtcgagca ggcagagatc aggaagaaag tgcagcaatt
    15901 cttttttatc aaagtgggga tgaacgtatc cattatttct ttaatcaact ttctagagaa
    15961 cagcgacaaa gttttgagct ttatttggaa tatgccacag agcaagctcc ctttgatgag
    16021 ctgcaaaaaa aatggatgga gctgatccag cagtctgaaa aaccagaaaa ttgggtggaa
    16081 cggctcaaac gtcaggaaaa agaaaaagaa ttccgcctgc agcagatgct tcgctatatt
    16141 aatgaagaaa actgccgcag aaaattcatc ttagcttatt ttggcgaaaa actctccgaa
    16201 aagccgcaga attgttgtga tattgatgga gcaaaagcag aacttctgcc aaagaggaag
    16261 cttttttcta agactgaaca acttcattgg gaaacgattt tactaaattt atttaaaaaa
    16321 aacatggaac attgattttt taggacaaga cactttttcc tatggtataa tacacacgat
    16381 agagtttagg aggtaaaaca gtgagcagaa aagatagaca tcaatctaca gaaaataacg
    16441 aaacacaaga accatgggaa caaccaatct acgatacaga tgatgaagtt tcatcaagaa
    16501 gttctcagag acaacaaaag aaaggaaata cattatttct ttcgctattt ttgattttac
    16561 tggtcttatg tatcgctatt ccagcaggtg cttatttctg gatcagaaat ggatcaagca
    16621 acaatgcaac agcagcaact tcttctgaaa cttcgacatc agtagtagaa agttctacaa
    16681 gtacaacaaa agaggagtca actgagactt caacagtaga atctagtaca atcgcggaag
    16741 aatctactgt gtctcaagat acaggtgtaa cgtcagaaag cagtattgca gcagagacaa
    16801 caccgacttc ctcaagtgag gcaacttatc aagcagggga ttcaagcagc aatgcagcag
    16861 aaacaacacc ttcttcatca gctgcagccg gttcaacaac gactgttcaa gcaggcgaag
    16921 gtccaaaaca agtggctgag cgtgcaggga tcaccactga tcaattgttt gaattgaatg
    16981 gcttagatcc aaacaacttt atgctttacc ctggtcaaga attaagaatt aaataataat
    17041 agccgcgctt tgcggctttt tttctgagaa aaaggagtta tatcatgaca caaatcagta
    17101 ttgcaatcga cgggcctgct tcatctggga aaagtaccgt tgccaagata ttagcaaaag
    17161 aattgaatta tatttataca gataccggtg ccatgtatcg cgcagtaact tatttagcta
    17221 ttacgaacaa tgtatctttt tcagatgaag aagggctagt ttcgctgatc aaaaagtatc
    17281 ctatctcttt ttcgcaacaa gaagatggac agcatgtatt catagcatca aaagatgtga
    17341 ctttagaaat ccgtcaacca gatgtcacaa aagctgtttc tgaagtctct gcacatggaa
    17401 aagtcagaga agaattagtt gctgtccaac gaaaaatagg ggaacgcggt ggcgttgtca
    17461 tggacggaag agacatcggg acagctgtat taccgaatgc agaagtgaaa atctttttag
    17521 tagcaagcgt aagtgagcgt gcagaaagac gtttcaaaga gaaccaagaa aaaggaattc
    17581 cgactgatct tgaaacactg acgaaagaga tacaggaaag agaccattat gattcaacaa
    17641 gggacgtctc tccgctgaag caagcagagg atgctgttcg tatcgatacg actggcaaat
    17701 cgattccaga agtcgttgca gcaataaaag ctgttgtttt gaaaaaaggt tatcttcttt
    17761 tttaaattaa gaaaaaaaat gaaaaacaat tgttttttca aggaaaacga acgaaatcgc
    17821 tttatttttt ttgaataatc aattaaacta aaaacagtac cgtacttacg gcagaatgtt
    17881 ggttaggagg acaagtgtta tgacagaatt tgatcaaaat caagagaaca accagtcaat
    17941 ggaagacgca atgaaaagcg tacaagaagt aaaagttggc gatgttgtta agggcgaagt
    18001 tttagtcatt gaagacaaac aagtgattgt cggaatcgaa ggggctggag ttgaaggagt
    18061 cgttccggca aaagaattat caactttgcc ggtagaagat atcagcgaag tagtaaaagt
    18121 cggtgatgta ttagatttag tcgttatcag tacgatcggc aaagataaag aaaacggcag
    18181 ctatctactt tcaaaacgcc gtttagatgc taaaaaagtt tgggaagata tcgaaagaga
    18241 tttccaagag ggcaaggtca tcgaagcacc tgttacaaat gttgtcaaag gcggtttagt
    18301 cgttgacgtc ggtgtacgtg gttttgtccc tgcatcaatg gtggaagatc attttgttgc
    18361 agacttttca gactataaag gcaaaacatt gagctttaaa atcatcgaaa tcgaaccatc
    18421 agaaaatcgt ttgatcttat cgcataaagc ggtagtggaa aacgaaagag aagaaaagaa
    18481 aaaagaaatt ctttcaacta tccatgaggg agatgttctc agtggacgtg ttgcacgtct
    18541 tactgatttt ggcgcatttg tcgacctagg cggaattgac ggtctagtcc atgtctcaga
    18601 gatttcacat agtcatgtag caaaaccaag tgatgtattg gctgtcaatg atgatgtaaa
    18661 tgtcaaagtg ttgtctatca atcctgaaca agaacgtgtt tccttgtcaa tcaaagacac
    18721 acagcctgga ccttggtcag aaatcgatac aaaagctcca gttggcacag tattagacgg
    18781 aacagttaaa cgtttgacaa gcttcggtgc ctttgttgaa gtgttccctg gagtagaagg
    18841 gctagtccat atctctcaaa tttcacataa acatattgcg acaccacatg aagtgcttca
    18901 tgaaggtgac gaagtgaaag tgaaagtatt agagatccat ccagaagaac atcgtattgc
    18961 attgagtatc aaagctttag aagaaaaacc agcttcagaa gaagaaccaa aaaatgttga
    19021 gtcttatgaa ttaccagaag aaaatactgg atttactatg ggagacattt taggtgatgc
    19081 gttgaaagaa gaagtagaag aaaaagcaac cgatgattct gaaaaataat tgattttgta
    19141 tctgtgactg agatgaaagc agctgctttt gtctcagttt ttcttttgaa aagcgattag
    19201 tatttcaata aggaataaaa atacttcgtt taattgacaa tctatgcttg aaagttggca
    19261 atgcattcgt taaactaagt gagattggaa acttgtgagg aggaaataat atggcaaatc
    19321 caacaattgc aatcgttggc cgcccgaacg tcggtaagtc tacgattttt aaccgcatcg
    19381 ctggtgagcg tatctcgatt gtcgaagata ctcctggagt aactcgtgat cgtatttatg
    19441 caaaaggtga atggttaggt cgagaattca gcgtgatcga cacaggcgga attgatcttg
    19501 gcgatgaacc ttttatggat cagatcaagc atcaagcaga aatcgcgatt gaagaagcag
    19561 atgtaatcat ttgtgtagtc agcggacgcg aaggcgtgac agacgcagat gagatggttg
    19621 cgaaaatttt atacagaagt aataaaccag tgattttagc agtcaataaa gtagataatc
    19681 cagaaatgag aaacgatatc tatgaatttt attccttagg gctaggcgat ccttatcctg
    19741 tttcaggaag ccatggacta gggattggtg atattttaga tgaagcagtg aagtatttct
    19801 ctgaagagac tgaagaagag gacgacgaca cgattaagtt tagtttgatt ggtcgcccaa
    19861 atgttggtaa atcctctttg atcaatgcga ttttaggaga agaacgtgta attgtttcgg
    19921 atattgaagg aacgacccga gacgcaatcg acacccactt cgtttctgaa agtggtcaga
    19981 aattcttaat gatagataca gctggtatgc gtaaacgcgg aaaagtatac gaaaatactg
    20041 aaaaatacag cgtgatgcgt gcaatgcgtg ccattgaccg ttctgatatc gttttgatgg
    20101 tactgaatgc tgaagaaggg attcgggaac aagacaagcg agtagcgggt tacgcccatg
    20161 aagctggtcg tggaattatt atcgtcgtga ataaatggga tcttgtaaag aaagaaacca
    20221 atacaatgcg tgatttcgaa caagagattc gtgatgaatt ccggtatctt gattacgctc
    20281 caatcgtttt tgtttctgca gtgactaaac aacggttgga acgtctgcct gaaatgatcg
    20341 aacaagtaag tatgaaccag aatcttcgta tctcttcggc tgtgttgaat gatatcatta
    20401 tggatgctgt agcaatcaat ccaacaccaa cagataaagg aaaacgcctg aagatttttt
    20461 atgctacaca agtcgctgtc aaaccaccta catttgtcgt ttttgtcaat gaagaggaat
    20521 tgatgcattt ttcttatgaa cgtttcttag aaaatcaaat cagaaaagca tttacttttg
    20581 aagggacgtc catccgaatt atccctcgta ggagaaaata ggcattttac catatcgacg
    20641 tttttttttc aaaagaaaaa gacagtttcg tttgttacgt ttctatgact aaaaaaagca
    20701 tgtgaaaatt ggtaaaaacc ttgctattaa gcggttcata tgatatcgtg ataacagaat
    20761 attgaagtta atcaatattt ctttttaaac ttctggacca ctcagaaagt tttaactaaa
    20821 tgatatttct atcatatctc ttgtggagga ggtgaatcta tccatggcaa acaaagcaga
    20881 attaatcgaa aaagttgctt cagctactga tttaactaaa aaagatgcaa ctgcagcagt
    20941 agatgctgta ttttcaacta ttcaagatgc tttagctaac ggcgaaaaag ttcaattaat
    21001 cggttttggt aactttgaag ttcgcgaacg cgcagctcgc aaaggtcgta acccacaaac
    21061 tggtgaagaa atcgaaattc cagctagcaa agtacctgca ttcaaaccag gtaaagcatt
    21121 aaaagacgct gttaaataag acagttgaaa aagcgcttgt ggctctaggg ctacaagtgc
    21181 ttttcttttt atttcctagc agaaaaggaa taaataagag acagagattc cgaatccgta
    21241 tgtcatacat agaaaacaca tgtattttat ttaattttta tttatttgca agttatgagg
    21301 tgagctttgc tcttatcttt tctagaattt gcttactaat ggtaaaataa aaataagaag
    21361 aggagggatg cgtatggaac ttgattatca agtagaagat gcgttgacga aagtgtttta
    21421 cggagaaacg ctagtcagtc agctaagaga actttccatt gatcagccaa tcttattttt
    21481 aacgaatcag cgctattatg atttgtttgc agataagatc aacccgttat ttgcaaatca
    21541 agcaaacaat gactggtata tttgtgccaa cactcaatgc aatcatttaa cagaattaaa
    21601 gaacctgctt gatttcgtca aacgctatcc cgaaaatcaa tccatgttta tcatcggatt
    21661 tggaaatgaa gggatcatgg atctggcagg tttttttcaa aaacatacaa cccttgattc
    21721 atcactatgg atgatccctg tatcgatccg ttctctaagc cgagcattaa caccgcagcg
    21781 gacgattttg cagctaccaa atcgtgtagt gctgcaaacc atcaatctgc cagaacgcat
    21841 cgtttatgat caaacaatca gcgagcggca ggtggacgga aagcagattg actttttgac
    21901 ttttatctgt tgcggaatga tttgtgatca tcgttttttg caaaatctgt ataaaaacta
    21961 tccaaataaa aagcaattaa cgactcggcc gtttgccggg atgatcgaag aaatgatcta
    22021 tttttatcaa ttagaggcag aaaacctgtc aaattatggt aaactgtttg aacaagcttt
    22081 ttatctgacc gaaaatggcc atctactttc tgctagtatg aaaaaatttt taggaatgat
    22141 gtttcatttc gtgtggaatc tccaagcata tccttttgat ttccaactaa aaaacttttt
    22201 tgtgtggctg gacttgcttg gctatccaat ccactggctg gaacagattt caaagatgga
    22261 gtatttggaa aaagttcttt ttttggcaga aaaaagcaaa aaaatacttg ttttagaaaa
    22321 aataggtata atagacggat accaacatcc aaacgaaaaa gaattattga gaacaatgac
    22381 ggtatttcaa caaatcgtca gtgagataag agggaattga aatgaataca aatagcgaaa
    22441 aaatgttgca agctttatca gatgaagact tgtcacaagc acaaatcttt ttagaaaaag
    22501 cattaaaaga agacccagca gatatattag ctgaactagg cgaagaattg ctggctattg
    22561 gctttttgat cgaagcaaaa cagatttttg aacaactgat caaccaatac ccagaaaatg
    22621 acggactgaa catcccgtta gcagaaattg caatcgaaga taatgagatc gacttagctt
    22681 ttgaatacct ggaaaaagtg gataagcaaa gtgattacta tccgcaaagc ttattggcga
    22741 tagctgattt gtaccaagtt atcggaatcc ctgaagtgag tgaagcaaaa ttgaaagaag
    22801 cagctgggtt gcttcctgat gaaccattga tccagtttgc gctagctgaa ttgtattttt
    22861 cagttgatcg tttcaaagag gcagcagtgg tttatgaaac attgttggca agcgggataa
    22921 atgaaatttc aggaatctcg atgcaggaac gtttaggaca atccttaagt atgcaaggag
    22981 aattcgaagc agctatcgta cctttagaag aagcactgaa agaagaaagg acagatgatc
    23041 gactgttcca gctagctttt acgaatcttc aattgaaaga aaatcagcag gcaatcaact
    23101 atctggaaga acttcgagaa gttaatccgc attatcaatc gttatatcta tatctaggac
    23161 aagctcttca agaagaagaa atgattgaag aagcacaaac agtgttagaa gaaggaatca
    23221 aagaaaatcc ttaccaagtc gaactttatc acctggcctc ggaaaatgct ttccgtctgc
    23281 atgatagaca gcgagcagaa gaacttttac tgaaagcttt agaagtagga gacaaacaag
    23341 atgaaacatt acttacttta agtaatcttt acttggatga tgaaagatat gaagatgtga
    23401 tacaagtgat caatcagatg gaagaaactg ctaatcctta tgcagaatgg aacctagccc
    23461 atgcttataa tgaattagaa gactttaccg ttgcagctgt acactatgaa caggcttatc
    23521 atgaactatc acatgaacca gatttcttga aagagtatgc ctttttctta agagaagaag
    23581 ggcagctgaa aagagcgcaa gaattattga cttattattt aacacttgaa ccaggagata
    23641 tggaggcact gtcattatta gatgacctaa cagagaggtg agaaaaatgc taattgatgt
    23701 acgggaaaag aagaattttt taacgtggat ggtcaatcat gtatctttta gccgaagaga
    23761 agttttttgg atcttgaatt atcttgccaa tcatgaagcg attttaagca atgtccactt
    23821 tgttgaaggg gcgaattttg cggatcgagg actgcagatc agtgatctat ctgttgaagg
    23881 ggaacccatg aaattgtttt tgcagggaaa agaatttacg gacacagacc agatttttca
    23941 tgaaatccgt ttgaattgga aaaagccttt atatgtggaa tgtatattcg aaaatgcttg
    24001 gcagacaaga gaatatctat ctatcttaga agacaatcca ttttcttctt gggattcttc
    24061 agtcagtgaa gaagatactg aattaattga acgatatttt caagaaaaag aagaagaggc
    24121 aaaactaaaa atgctttatg ctcaaatcga tcaagcacta gaagatggag atagggatgc
    24181 ttttttgaaa ttatctgatg aagttaaccg aaagatcctt ttgcagacac aaaagaaaag
    24241 taaccgggta agtgaacata acaattagtt aaaaatagta tagtactaaa aaagacaaag
    24301 gtcagctgga tgtggaatct ttcctcatag cctatctttg tcttttttct tatagttttt
    24361 tcagtaatct tgtctgtggc ttcaaataag taaagccttg cccttccact tcatgcacat
    24421 tgatgattga aaggaaagct ttttcgtcaa tctcgtggac gatcctctta gcttcagtta
    24481 attcactaga gctaacgacc atatagatga ttttttttga attaccagag aatgcgcctt
    24541 ctccagaaag gaaagtcaca cctctttgca gaccggtcat cagtagtggt gcaatctctt
    24601 cgcttttgtt ggagacgaca agtagacctt ttgcagaata tccgccatct aagatcgaat
    24661 caatcacacg actgaaaaca tacgaaacaa tcaacgtata catcatacgt ttcaagtcga
    24721 tataggttaa tgaagcaagt aagacgatga tatcaaatgc caatagggaa cgccccatag
    24781 tgatgccgta attttgttct aaaatccgag cgataatatc actgccgcct gttgttccac
    24841 ccatgcggta gacgatcccg cttcctacac ctgcaactaa acccgcaagt agagaaacga
    24901 tcagtagatc atgctgtaaa ttgatttcta gtggaaccct ttgccagatc cataaaaaga
    24961 atgacaaaga aagtgtacct agaacagtat aggccaagga acgtttaccg aggatctttc
    25021 ctcctagtaa aatcaatggg atgttgatta ataaagtcgt ataagctgga tcaataccaa
    25081 ataaagctct taagatcaag gtgatccctg agactccccc ttctgctaaa tgattagcga
    25141 tgttcaagta aacaagacca aatgcataaa tacttgttcc tataatgata aaaagaactt
    25201 cttttactgt ttttcgtttg accatatgaa ttccagcttt caattatgta ttttcacaga
    25261 atttccattt agaaattcct tcctaaatgt agcatggaaa ttcgattttc tcaattgctc
    25321 ttatctagga tttgcgttaa agttttggta agatagtttt ggagggaaaa agatggataa
    25381 aaagcgttct ttatacagta tgcagcagga agtggatgaa tatatccagc aattcaaggt
    25441 ggggtatttc tctccattag ctcagatggc tcgattaact gaagaagtag gtgaattggc
    25501 aagagaagtc aaccacactt atggagaaaa aagcaaaaag gcttctgaac cagttaactc
    25561 ggtagcagaa gaacttggtg atgttttatt cgttacgatg atcatggcta attcattgaa
    25621 tatcgactta actgaagttt ttgaaaaaaa catggaaaaa ttcaatcaaa gagaccataa
    25681 tcgctttgag cgtaaggacg agtaaacaag atgaagtggt cctggccttg aagactctga
    25741 aacggacttg tcaaacagtc accatgcaac tgagtcttta tctcaggaca agaaacacgc
    25801 aggaaaggtt ggaaaaatga aattaaaaca attacctctg gaataccaaa aggcaattcc
    25861 agttctgaaa aaattagaga atgcaggata tgaagcttat tttgtaggtg gaagtgttcg
    25921 tgatatcttg ctgaaccagc caatccatga tgtggatatc gcaacaagcg catttccaga
    25981 agaaatcaaa cagctttttc caaaaacgat agatattggg atcgaacatg gaactgtgtt
    26041 ggtattagaa aatgatgaac aatatgagat caccacattt cggacggaat caacttatca
    26101 ggactaccgt agaccagatc atgtcgaatt cgttcgttcg ttggcagaag atttgaaaag
    26161 aagagatttt acgatcaatg cgtttgcttt gaaagaagat ggagaaatca tcgatttgtt
    26221 cgatggtctg caagatctag aagaacagat cttgcgagct gtaggcaatc ctcatgagcg
    26281 gttccatgaa gatgccttac gaatgatgcg cgggttgcgt tttgtcagtc aactagggtt
    26341 caaattagag gaagaaacct tctacgccat caaagaaaac cacgagctat taggtaaaat
    26401 atctgtagaa cgtataaatg tggaattcat caagatgctc ttgggaaatg aacgggccgc
    26461 gggaatccat gcatttattg aaacagagtg ctacatttgc tgtcccggtt tgaaagaaga
    26521 aggcgaagca cttttacgtt ttgctgaatt accatccgtc ccattgaaag aagagagcca
    26581 agcatgggca ttattgatca gccaactgga tctaaaagaa aatgagattc gttccttttt
    26641 gaaatcgtgg aagtgttcta accaaatgat taaagacgtt caagcacttg tccaaggact
    26701 acgacaacga gaaactggag ctttggaacc attcgaattg tacttcttag gcaaaaaaca
    26761 tgcactgcaa gtagaagaat tgatgatcta ttttgacgaa acacctcaga tggatgccgt
    26821 aggagaatcc tatgatcgtt tacctatcaa gtcattaagc gaattagcag tagatggtaa
    26881 aatcttgcta gaagaaactg gaaaaaaacc tggtaagtgg gttagtgaag ctttgcaatt
    26941 agctgaaaaa gccgtcgtag aacaaagaat aaaaaatgat aaaaaacagg ttttggattt
    27001 tttatcgaaa gaaaaattga tttaaaaaac actagactgt atctgctcag ctttgttggc
    27061 tacttaaagt gggcagatac gtcattagaa gaaaaaacat aagatgtgac cacttacata
    27121 aatttgtgct aaactgaggt agtacaagaa taaattacat cataaaggag tctttttaat
    27181 gagtaaagaa atgacagcgc taaaatttta ttttagaaat ggagaaacct ggacgatcaa
    27241 ccgccgtcat atcggcgatc tttggatcaa acaaatcaca acaagttttg gacgaatcaa
    27301 tggcagtgaa tttgttgaaa tccatccgtg tgctggattt aaaatcgaaa tcttccatga
    27361 aggggacgca gttgcgacac atgatatcaa cctaggcgga ctggaaatgg gcatgtttaa
    27421 tcgcgcattg aaatacgaag atatcgagcg aatggaaatc ctttaccgaa atggcacacc
    27481 tgatcttgtg tacttcccat atctagataa aggaacagaa ggattagaca atcaatatca
    27541 atcaacaaaa atcagcgaaa aaacaggtaa cttgtatatc gtgatcaatc cagatcaacg
    27601 tgtagaagat gtatacgggg aatttttcga ataaatcgat ttttacgcaa aaatgaggct
    27661 gtgaaagaga tgtgtcttat tactactgat aggctgtctc ttccacaccg tttattcaga
    27721 ataagggact gtgacaagat ttttgtcgca gccccatttt tttgttgtga taaaagaaca
    27781 agaaaggcag gaatgggaaa gtaaacatcc ttttgtcaga aacttttatc tttcttaagt
    27841 tttacttgat atctgatact atttggaata gaatgaggcg ttgaaaaatt tatggaaatt
    27901 tctaactagc aataaacgat gaaacaatga aatatttttc atccaaaaag aactttgcta
    27961 taatgatgga agtttgtgta taggagatct ggaaaatgag agagagtttt gaacaacaaa
    28021 aaaaactgtt gcatgatcgc tatggcgccc tttcgatgga tgaccgccgt caaatacttt
    28081 gtaagttgcg taaaagaaat atattgatgt atcgtcagtt ggaacgtctg aaacatgatc
    28141 tgcttcgttt ggagtcaaaa cgtgtacagt gtgaattaga ggggaaccaa acacaggttg
    28201 aagtagtcga aaccaaaatt ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa
    28261 ataaaaaata aaggggatgg acgatggaat ttgctgagtt agttattgta tttgcagtaa
    28321 cgatcacctt atcgaacata gctagccgca ttttgccaat gattccagct ccactgatcc
    28381 aaattttcct tggagtggta ttaggtctga cagagtgggg acaatcaatc gattttgaac
    28441 ctgaattgtt cttagttatg attattgcac cattgttatt tagagaagga gaaaaagcag
    28501 atatttcttc gattctcaaa aatttcggga caattttgtt cttggcattt ggaggagtca
    28561 ttttgaccct tgtcggggta ggtgcgacgt tatcattcct gttgccaagt gtcccacttg
    28621 ctgcctgctt tgcttttgga gcagcgttag gtccgacaga tgcggtagca gtcagttctc
    28681 tttctggacg agtgaatatc cctaaaaagg cgatgcatat tttggaagga gaaggtcttt
    28741 tgaatgatgc atctggtgtt actgccttcc agtttgctct tggtgcgctg atcaccggat
    28801 ctttttcagc agtaaacgcg gggatgagtc tagtagtttc tagtattggg ggagctttga
    28861 tcggcttttt gcttgtctgg ttcaaacaaa aaataattca tttgatcgaa aaagcatcag
    28921 cacaagatgt cacagggtat ctgctgatcg aacttttgct tccttttcta gcctatgtat
    28981 tggcagaggc atttgatgta tctggaatca ttgctgcagt ggcagcaggt attctccaag
    29041 cttctggttt taggaaaatc tctgtgtttg atgcagaatt gtcgagcttg tcccacagta
    29101 catggacaac tattgctttt acactgaatg cattagtatt tatattttta ggaatcgaat
    29161 tgacgcaagt gttctcgcca gtatggggag acggtttata tccaaacggt cttttattag
    29221 cgattatcgt attaatctct gtcatgcttt ttgtgatcag attcatttct atcagtcttt
    29281 tttatgtatt caaagatggc agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct
    29341 taacatttgg cggtgtaaag ggaacagtca gtttggctac gatttttatt ttgccatttt
    29401 caatcaataa tatgatgttt taccagcggt ctttgctttt gtttctgacg gcgggtgtca
    29461 tcttagtgac tttggtcata gggatcattg tcttgcctat gctgacggaa acagaggaag
    29521 cgaaaagcac ggatttgaat gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa
    29581 aagaaataaa ggaaattgat cgtaatacaa aagaattttt agccactgaa gcagtgatag
    29641 aaaattatca agaacggata cgtgatctgt acttggaaga tttaaccgat gatgaaaagc
    29701 aagaagtaca agaaatacaa gcactgattt tgtcgatcga acgggatgga ttggatgaaa
    29761 gttatcgttc gggcaagctt tcttctaatg gttatcgttt ttactcccgt tttctttctc
    29821 gattcgaaca ttctatcaca agccagattt tgtcttttat cggcttttgg ttcattgttg
    29881 tccgtaggtt aatgcgaatc gttctccatc cgaaaatgtt ttggcaaaga agatatgctg
    29941 atcgtcagac atttattagt gatgaagata tccaagaaat cagagagaca tatcaaaaaa
    30001 atactcagtt gattattgaa agtttagata acttgactga tatttatgat gaaacgatgg
    30061 ttcaattttt tatccagcaa cgcaaacttg aaggaataaa aatgaactcg gggaatttga
    30121 tttcttcgtg gatgatccag caagatagtc tgtttacgaa aaaaatgcta agaggttatt
    30181 atcttgaaag aaaaatcatt gatgaatacg aagttgctga aaaaatcacg acgttttctg
    30241 cgaacagcta tcgcaggaat atcaatttgt tagaatctta tacgatgaac aaaccatctg
    30301 ataacttttc tttccgattt gcgtttcgag caaagtcaaa aaaaacgagt ccaataaaat
    30361 aaaaaggtaa aaattttcaa gttttataaa aaacaaaaca tatcagaaca ggatcgaatc
    30421 ggtcctgttc tttttttgag aacacaaaaa gaacaaatac caaaaaaatt ttcctgaagt
    30481 tcactatttt tatgatacaa tatccaagat aaggagggaa catgttgaaa gaattaaaag
    30541 taactgacct caaaaaaact tatggtgaaa aagatttgtt tgaccagatt tcttttttgg
    30601 tccacgaaaa agaccgtatc gggttgattg ggacgaacgg gacgggtaaa acaagtctct
    30661 taaatattat tgccggtatc gatagcagcg acggtgatcg gcaaacagtg ttttatccaa
    30721 ctgattaccg tattggttat ctttctcaag cagctgaatt ttccgatgaa ttaactgttc
    30781 tccaagcagt ctttcaagga aacagcccct tgatccaaac tgttcgagca tatgaggaag
    30841 cattgatcga actaggggaa aacggagaag accctgatgt gcaaaaacgt tatgctaaag
    30901 cagaagaaca aatgaataaa gaagatgctt ggactacaga taccaacgca aagattattt
    30961 tgcaaaaatt aggaatcagt gaattagata aaaaaatcag tactttatca ggtggtcaga
    31021 aaaaacgagt tagtctggcg caagtgttga ttgatgaacc cgatttacta ttactagacg
    31081 aaccaacaaa ccatttagat tatgaagcga tcgagtggtt ggaaaactat ttgaaacaat
    31141 accgtggcgc actattgatg gttacccacg atcgttattt tctagataga gtagcaaacc
    31201 gaatcttcga actttcattt ggaaaattat acgagtataa aggaaattat gaagcttacg
    31261 tcttagaaaa agcagaacgt gatcgtgtag aagtagaaca agaagaaaag cgcaagcggc
    31321 tttataaaca ggaattagct tggatgaggg ctggcgtaca ggcacgtggc accaagcagc
    31381 aagcacggat caaccgtttc gaagatctaa aggagaactt gtaccaagta aaccaagaag
    31441 atgatttaga gctgaaccta gcgacacaac gtttagggaa aaaagtactg gaaatcaaag
    31501 atggcagtta ccgtataaat gaccaaacac tgctagaaca tttagattta ttgattcaat
    31561 ctcgggaaag actaggtatt actggtaaaa atggtgcggg aaaatcgaca ttgcttaata
    31621 ttctagctgg cagaattccc ctggatagcg gtacaatgtc tatcggtgaa acggttcatc
    31681 ttgcgtatta tacacaagaa aatgaagaaa tggcaccaga taaacggatg attgcttact
    31741 tacaagaagc tgctgaagaa gcaaaaacag cagatggcag tcaaatcgga gtagcagaat
    31801 tacttgaacg atttttattt ccaagattta tgcatgggac actgataggc aaactttcag
    31861 gcggagaaaa acgccggtta tttttgttga aactgctgat tcagcaacct aatgttttat
    31921 tattggatga accgaccaat gatttggaca tagcaacatt gacgattttg gaagattatt
    31981 ttagaagttt tccaggtgct gtgatcactg tttcccatga ccgttatttc ttggacaagg
    32041 tagcagataa gttattagta ttccaaggaa acggaaaaca agaactttat tacggaaaca
    32101 tgtccagtta tttactgaag caaaaagaga cccagcagcc tgctgaaaaa gcgaaaccaa
    32161 aaatacagag caaggaacct gctgaaaaga agaaattatc ttacatggaa caaaaagaat
    32221 gggaaacgat tgaagatgag attgcagaac ttgaagaaaa aatctcattg ctccaagaag
    32281 agatgaatca tcaaggagat aatttcactc gattacaaga attgcaaaac gacgtatctg
    32341 aaacagaagc acaacttgaa gaaaaaatgg cacggtggga atacttgagt gaatgggtag
    32401 aagattagga ggaaaaagga tggaacaagc gtatcttgac ttaggaagaa aattgttgga
    32461 agaagggcat cgtaaagaag acagaacagg cacaggcaca ttaagcttat tcggatacca
    32521 aatgcggttt gatctgcaaa aagggtttcc tttgttaaca acgaaacgag taccttttgg
    32581 attgatcaaa agcgaattgc tttggttttt aaaaggagat acgaatatcc gctatctatt
    32641 acaaaataaa aaccatattt gggatgaatg ggcatttgaa cgctacatca aaagtccaga
    32701 ctataatgga ccagatatga ctgattttgg ccatagatgt ttgacagatc ctgaattcaa
    32761 tgaagaatat aaaaagcaga gcaagctttt ttgcgagaag atcctaaccg atgattcctt
    32821 tgctgaaaca tacggcgatc ttggacacat ctacggctat caatggcgtc actgggaaac
    32881 aaaagacggt ggctttatcg atcaaatcaa acaagtgatc gaagcaatca aaaagactcc
    32941 tgattctaga agattgatcg tttctgcatg gaatccggaa gatgttccta gcatggcttt
    33001 gcctccttgt catacgatgt ttcaatttta tgttcaagag ggacggctaa gctgccaatt
    33061 gtatcaacgc agtggggatg tgtttctagg cgttccgttc aacattgcaa gttatgcgtt
    33121 gctgactcac ctaattgcac atgaaacagg tttagcggtt ggcgaatttg ttcacacatt
    33181 aggagatgct catctttata tcaatcatat cgaacaaatg aaagaacaat tgagccgaga
    33241 aatacgcgca tttccgactt tagttttgaa tcaggaaaag cagtcagtat ttgattttga
    33301 gatggaagat atccaattag aaggatatga cccacatccg gcgatcaaag ctcctatcgc
    33361 tgtttagaat cagaaaggat gagaaaatgt tagctgcaat ttgggcacag gatgaaaacg
    33421 gattgattgg aaaagaagac cgtcttcctt ggcgtttgcc aaatgatctg aaattcttca
    33481 aacaaatgac agaagcaaat acactagtga tgggacgaaa aacattcgaa gggatgggta
    33541 gccgtccgtt gccgaatcgt cagacgattg ttttgacgag agattcttcc tataaagcag
    33601 aaggcgtgca tgtcatgcat agtgtggaag acgtgcttgc tctggaaaaa gaaacagacg
    33661 gtatcttttt catcgctgga gggtcagctg tttatcaaga atttctgcca ttttgtacga
    33721 tattataccg tacagtcatc catcatgtat tcgatggaga tgcttatttt ccacctgttg
    33781 actggtctga ttggtcactg atcaatacta gtcaaggaga aatggatgaa aaaaatcgtt
    33841 atccgcatca atttgaaacg tatcagcgta atgagaatcc tgtaaaaagc gggacaggcg
    33901 aattgacgat ataaaaaagc tatggataga ctagctgtta tttagagctg tcccatccat
    33961 agctttattt tttttattaa gtaaagaata aaacagagaa gtacataaaa cctgcaccta
    34021 acataacaaa caaatgccaa acaacatgca tatagcggat agaattcaag ctataaaaga
    34081 atgcgcctat cgtgtaagaa agcccgcctg ctaccatcaa aataacacca gtcattccaa
    34141 gagagtgata aagaggaaca gcagccacga cacagagcca ccccataaaa atatagatga
    34201 tcgtagatac ttttgatact ctctcccgtt tatgcaacgt caacgcttta tatataatgc
    34261 caagaatcgc aaacaaccag attaatacaa ataaagtcca acctagccat cctcggatac
    34321 tcagcaaaca aaaaggagta taacttcctg caatcaaaag aaaaatcgaa ctatgatcga
    34381 atacttggaa cacctttttt gcttttgtaa aaatcaatga atgaaacaaa gtagacgtta
    34441 aaaataaaag aatcatcatc gatccataga tagcataaga gacaatatgg agggaggaat
    34501 gcaagtgggc accttttact aaaagaacca ctagtcccgc gatactcaat ccagtcccaa
    34561 ttccatgggt gactgcatta aacacttcat ttaatatttc atacgttcga ttaaatttcg
    34621 ttttttccat gaaaacggct cctttactag gtgtaattaa tgaaaaatca ttatttgtct
    34681 gttatactaa aaatagaaat tgtcttacat gtagtttaac atgaataagg catcttgtta
    34741 aagaaagaga gagagaaaat aatgacaaat gtaaaaatcg taacagattc ttcatgtacg
    34801 atggaaaaaa atgtacgcga tgctttaggc attcgtgtca tgccgctatc catcatgatc
    34861 gacggaacag tatatcccga tgatgaccaa ctagacggcg aaagatttat ggaaatgatg
    34921 gcgaaagcaa aaaatctgcc caaaacaagc caaccaccaa ttggagaatt catctcgatg
    34981 tatgatgagc taggagaaga cggcagtgaa gtgatttcga tccatatgac aaaaggcttg
    35041 agtggaacag tagaagcagc aagacaagcc agtaatctaa caaaaacgaa agtaacggtt
    35101 atcgacagtg ataccactga ccaaggatta tcttttcagg taattcgagc agctgaatta
    35161 gcaaaagaag gtaaaacagt agagaaaatc cttgaagaag tcaaaaaagt acaagacaat
    35221 acaaaattat acatcggaat atccacacta gataacttgg taaaaggtgg aagaatcagc
    35281 cgaacaactg gtttgctgtc tagtgttttg aacatacgcg tggtgatgaa tttcgcccat
    35341 tctgaattaa tccctgtaac taaggggcga ggaaagaaaa cgttcgataa atggtttaat
    35401 aatttgaaaa atgaactgaa agaattgcca aatgtgcgac aaatcggtat ttcacatgcg
    35461 gatgcaaaag aattggcgca agaattcaaa gagggtttgc aagaattatt cccggacatg
    35521 catatccctg tactgcatac gaatccggtt atcgctacgc atactggtaa agacgcgttt
    35581 gcaatcatgt attatgtaga ttagtcgaaa agaggataag ccctcagcct gaaaggcttg
    35641 cagcttattc tctttttctt tataaaatta ggaaaataat tgttttcttt tttcataaag
    35701 actacctttt tgataaaatt tttgataaca ttagaaccaa tcgaatacaa gaggtgaaaa
    35761 aatgaaatta acgaaacgac atttgatcct cgttttgttc ccattattaa ttgcgatcat
    35821 catctatgca ggtttagctg cttctatacc gaaagctggc ccactaatga aaccagtaac
    35881 tactgtgaga gaagatccaa ataaaaaaga aaaacttcac tacatagcta tcggagattc
    35941 gctgactgaa ggagtaggag atcaaaccaa gcaaggagga ttcgttcccc ttgtcgcaaa
    36001 tgatctaaaa gaccgttata atttgaccgc ggttgaaaca gaaaactatg gtgtaaatgg
    36061 tgaacgaagt gaccagatct taaagcgagt aaaaacaaaa gaagaaattc gaaacaatat
    36121 caaaacagct gattttatca cactgactgt tggcggtaat gatttgatga aagttatcca
    36181 aaataattta ttcgggttaa cggtcaattc ctttaaaaag ccactgaaaa actatcagga
    36241 aaatgtcaca gaactattgg aagaatttcg atccctcaat aaaaccgcac ctatttacat
    36301 tttaggcatc tacaatccat tttatttgaa ctttcctgag atcactgaca tgcagacaat
    36361 tgttaacaat tggaacaaag gaacagaaac ggtgacaaaa aaaatgccga actgctactt
    36421 tatcccaatc aatgatttgc tttatcaagg attgaatcag gaagtaggtg tcacagaaga
    36481 aggaacagat caaacaacta gcacgacaga aaatctaaat ggcatcaaaa ataatgtgct
    36541 ctatgaagaa gatcatttcc atccaaataa tttaggttat caattgatgg caaatgcagt
    36601 gagagatcag ctgatcgaga cacaaaacca atggttagag aaggaggatg atcaataaac
    36661 atgaaaacaa gatctgacgt atcttcaaac aaaacaaaga aaacaaatta ctggaaattc
    36721 gccttcctta tccttcttgg ggtagtactt ggttcgaccg tatttttagg cagccgtatt
    36781 tttgctaatc gggaaccaga actcccagaa ataccagctc ttacggaaag acaaggagat
    36841 cctgtcttaa cgatcaattc aaataaagaa aaagtcaatc aaatcatcag ctttttcctt
    36901 agtgagtatc aaaaagatag tgatattgaa tataaatttt atttggaaaa tgaagcacta
    36961 ctcaatggta ctttcgaagt actaggtttt cctatcgatt tctatttata ttttgatccg
    37021 tatgtgatgg aaaacggaaa tgtccaacta aaagctagaa gtctttctat cggaacattg
    37081 agtttgccaa tcaaagatgt gatgaatatg atcaagagaa actataaatt accagattgg
    37141 atcgaagtca atacagatga tctgactatc atgcttaggt tggatcaatt ccgaatgcaa
    37201 aacgggatgt atattaaagc agataaaatc gacttagtaa atgatgacat tcggtttagt
    37261 ctatatttgc caaaagatac ctctgatgct tcaaacaact caaataccaa caaaactagc
    37321 accagcagta ctgaaagcaa gacaaaagaa aataaaaaat catcggatga gtagtattag
    37381 agggggaatc ataatggaac gagcaatatt tgcaggaggc tgcttctggt gtatgatcca
    37441 gcctttcgac acgcttcctg gtatccatac agtgatgtca ggatatactg gagggcatgt
    37501 accagatccg acttatgaac aagtaaaaag ccaaacgact ggacatacag aagcagtgga
    37561 gattatctat gatccaacat tgatttcata tgaacaatta gtagaacttt actggcagca
    37621 aactgatcca actgacgctt tcggacaatt cgaggatcgc ggtgacaatt atcgcccggt
    37681 gatcttttat ttgacagaag aacagcggat cattgctgaa aaaagtaaga aacagttagc
    37741 tgaaagtgga cgtttcgaac agccaatcgt tacaaaaatc gaaccagccc aaccatttta
    37801 tctagcagaa gaagaccatc aagagtatta taaaaagcat cctgaaaatt ttaaaagaaa
    37861 ccatgcacgc cgggctgcct ttatcgaaga aaattggagg aacgaagggt gaatcgaagc
    37921 ttttttcatt atttaatgac actgcgaggc ggtggcacac atgatgcttt atctgttttc
    37981 gctacagaag ccggtaaaga catccaattt cctaagcaca gctctagcta cgaagaaatt
    38041 tctaattatc tggaactgaa tgtcgactat ttgccgtcca tggatatctt tgacgaagca
    38101 tgggaaaaat atttagaaaa taatcatgac atataagaga tagttttgta ctttatgatc
    38161 aaaaaagttt tttgtccaat agcctaattg aaagaattat ttttatttca gctatctagt
    38221 cgcatttttt tgatattttt cttataataa agaaagaatg aaaaaaagag ggatggattg
    38281 atagagattg acaaaaaaac aacgctatat taatcgcttc tggcttgtat tcaaatactt
    38341 acttgtttct gtattccttg cgttcatgtt gcggggattt ttattcatcc cggtaccagt
    38401 ggaaggcaat tcaatggaaa acgtgttgaa gcaaggtgat atggttgtga tggaaaaatt
    38461 ttcggagatc agacgatttg atattgttgt ttttcaatta gcagatggca cgatttatat
    38521 caaacgagta atcggtcttc caggagaaaa tgtcagctac caaaatgatc aattgaagat
    38581 caatgggaaa gtagttaagg aaccttatct aactaaaaac ctgaaatcag atcatgcaaa
    38641 tgcatcctat acgacggact ttacactaca agagctaacg ggacaatcca agcttccgga
    38701 agataattat tttgtacttg gagataatcg aagagtttca aaagatagtc gttcgtttgg
    38761 cacgatcaat aaaactgata ttctaggtaa agcaagattt gtctattatc cacttgatga
    38821 aataaaatgg atccagtgag aaacaataaa gcgtgcagtc tgttgagact gcacgcttta
    38881 ttgtttaatc caaaatcagt ttactacctt agtacgtaaa atcaaacagc tcgtttaaac
    38941 acttttctag cctctaaagt tttgataaat gaataaacaa ccattatttg aatagggatt
    39001 tggatcatat tcctgatgaa tctacctaga aaaattactt tgaaatctgc accaaacaaa
    39061 acagacatca gccaagaatt gataaaaagg ctgactggga tcataatcaa taattgggca
    39121 gcgatgacgg aaggcaggtt tatcttttta tgatataaaa ataagccatt aataaatcca
    39181 ccaaggaaag ccgtgataag aaaagcagct tgaaacgttc ctgaaccaaa tatcgtgaaa
    39241 tttaaagtgt tcgacaacat ttcaagcaaa ccagcccaga tcggtccata tagataaccc
    39301 ataagcgcca tacttaaaaa tccgaatgtg atttgaaaat aagcaggacc aatcgctatc
    39361 ttgtataaga tgacatcaag ggctaataaa agagcagagg tcgacaacaa tcttgcattc
    39421 atttcgggca atctaaaaac gtcttttgca atcaaagtgt tcaaaatatt ctctcctttg
    39481 tcagttaata ataaaaggat accataaaat caccaatgtg tcatgacaca ttggtgattt
    39541 acatacactg gcatctaaca attaaaaaaa gggtgagaag actgctttta taaactgtaa
    39601 aaaagagatt gtacgaataa aaaaaataga ggcggagaat acttatcgta tcatccgcct
    39661 ttgttttttt gttataaaaa acgtcagatt agttatctgc ttacatcttc atccatacgg
    39721 gttctaatgt atttccgtct agatcttgga cttctaatga aaacatttct tcttcaggca
    39781 ttcccatatc tacttcgtgg aaagtaccac cattttcttt tgcaatttga gcaaattttt
    39841 ttacttcatc cgcattttca acactaaacg aaatcaaagc accacttgtt tttttattat
    39901 cagctatttc tttatctttt ataaattttt tataaaaatc atgagagaga agcattatcc
    39961 aaaaagtgtc atcccatacc attgaaccag cttcttcagt agaaaattct tcattttttt
    40021 taaaacccag cttttcataa aaagcaacgg aacgattgac atcagcaaca ggaaaattaa
    40081 caaaaaccat actattcatt tgtttcgtct cctttctgtt ttgttcgtct atcatctctt
    40141 atttaggaaa cgctgtcaaa aatttagatt catatataga aaccaaaata aaaatttgca
    40201 tcatcaaaaa agtgaagatg tgatagaatg aggactggtt cactttcact taactaatag
    40261 atggaatttc tttataataa aaaagtgaat ttatattgaa aaagaaaaaa acaagtaaag
    40321 ccggggaata ttccggtatg aaaggaatgg agagaacaat gaccatccaa tggtttccgg
    40381 gacatatggc aaaagcccga cgagaagttt ctgaaaaaat gaaatatgtg gacatcgtat
    40441 ttgaattggt agacgcacga ttacctttat cttctcgtaa tccaatgcta gatcagatca
    40501 ttcagcaaaa acctcgcctt gttttattga ataaagcgga tctggcagat ccacaacaga
    40561 caaagctgtg gcaagaatat tttcgtaaac aaggccatct tgcattagct atcacggcac
    40621 aagaaagtaa aggaattaaa ggtctagtac ctgcggctaa agaagcattg aaagaaaaac
    40681 gggcaagaga tgctgctcgg gggatcaagc ctcgcgctat tcgggcaatg gtcttaggta
    40741 ttccaaatgt tgggaaatct acgttgatga atcgactagt aggcaagaaa attgcacaga
    40801 caggaaataa gcctggcgta acaaaaggtc agcagtggct tcgtttaggt aatgagttgg
    40861 aattgttaga cacaccaggg attttatggc ccaaatttga agatgaagaa atcggcaaaa
    40921 aactagcgct gacaggcgcg atcaaagacc agcttgtcca tttagatgat ttagcgattt
    40981 acggattaga cttttttgcc cgatattatt caggaagaat cagcgatcgt tatggtttat
    41041 caatcgagca agaagaaaaa ttggcaccgg aattactgat ggatattaca gcaaaacgtg
    41101 ggtataaaga agactatgat cgtggcagcg aattagtcgt gcatgatatt cgtcaaggaa
    41161 aacttggacg atacacactg gatcgttgcg aagaaatgga gcaaaaaagt gatgactgaa
    41221 tccatccaaa gtatcaaaga acgattgaaa agcatacaga gaatagatga tcctacacta
    41281 gaatcattaa gaaatgatcc acgcaaaggc gtgcagcaag cactggcttc ttttgaacga
    41341 cgattgaaaa aggaagcatt aattaaggaa aaatatgaag aaatgttttg ctttgaaaag
    41401 caagggtacg cacaaggtca ccaatggatt gccggcattg atgaagtagg aagaggacca
    41461 ttagctggac ctgtcgtggc agctgcagtt atcttgccga aaaatgagat catattaggg
    41521 ctgaatgact ccaaacaatt atcagagaaa aagcgagaac ttctttatca acaagttcaa
    41581 gaaaaagccc tggcaatcgg gatcgggatc gttgatcagc agacaatcga tgatgtgaat
    41641 atttatgaag caagtaaatt agcgatgatc caagcagtag aaaaattgtc cattgtacca
    41701 agctatttgt tgatcgatgc gatgaccttg gatttgccta ttcagcagga aaaaataatc
    41761 aaaggggatg cacgatctgt gtcgattgct gctgcaagta tcattgctaa agtatatcga
    41821 gaccaattga tgaaagaata tgacgtgctt tatcctggat atggtttctc gcataatgca
    41881 ggttatggaa caaaggaaca cttagaagga cttgaacgac aaggaattac gccaatccat
    41941 cgtaaaagtt ttgcacctgt aaaaaaatat ttacaataaa aaacagccgc ttttacgtat
    42001 cttattagaa agagagacga aaggcggttt tatgatgtat caaatagaag aaaatttatt
    42061 gaaacttgct tatacaaaag gaatcagtag tcaaggaaaa tgggcacttg ctaactggat
    42121 gatgcagtat ccttataaag aaatcggatt tgaagacatc attaagatag gtaaaataac
    42181 caaacaccgg gagctgtttc gcaaaagttg gcaggaaatt cacgctaact ggggcaaaat
    42241 ccaatcgaaa cagtcgttta tcacttgctt tgatcccaaa tatccccctc agctgcttca
    42301 cttgacctat ccgccaattg tactgtttta taatggtgat ctatctttac tttcttgtaa
    42361 catgttaagc gttgttggtg gaagacacac ttctgcttta gccaaaaaaa cagtttatta
    42421 tttactaaaa ccagtgatag aagctggata tgtgatcgtc agtggctgcg cgaaagggat
    42481 agatacatac ggtcaccaag cagcaatctc tcatggtgga cggacaatcg cagtcattgg
    42541 aacagggata gagcaagtct atcctgctga aaatcgtgac ttgcaagagg tgattgcaaa
    42601 tgatcatctt cttttgagtg aatatcctcc tcaatccggc ccgaaaaagc attgttttcc
    42661 aatgagaaat cggattatcg cagctttgtc taaaggaaca tgtgtctttg aagccaagaa
    42721 aaaaagcggc tctctgataa cagcacagca agcattggat cttggaagaa cagttttttc
    42781 tgttccagga aatattttaa cagaacattc taccggatgt catcatttga tccaagacgg
    42841 tgcagtttgc acggtttcgg gtgaagatat cttggcagaa tttaaagaat aacaccatac
    42901 ttttcatttg cttttcttga caaacgtttt ctgaatggat atgattagct acgatttgta
    42961 tgaatcctct tttaatagta ggaacacgac aatggaaagg agtccattca gtaatggcgt
    43021 ataaatattt agtgattgta gaatcacctg caaaagccaa gacaattgag aaatatcttg
    43081 gacgaaacta taaagtaatg gccagtgttg gccatataag agatttaccg aaaagtaaaa
    43141 tgggtatcga ttttgaaaac aattatgaac cccattatat ttctatacgc ggaaaaggcg
    43201 atgtcatcaa aagcctgaaa gccgcagcaa aaaaagctca aaaagtttac ttggcaagtg
    43261 acccggatag agaaggagaa gcgattgctt ggcatttagc gtacctactt gggttggatc
    43321 tgaaagaaaa aaatcgggtg gtcttcaatg aaatcacaaa agacgcagtc aaagcagctt
    43381 ttaaggaacc aagaacgatc gatgtagatt tagtagatgc acagcaagct cgtcgtacct
    43441 tagacagaat cgttggttat tcgatcagtc ctattctctg gcgtaaggtc aagaaagggt
    43501 taagtgcagg acgtgtccaa tctgtcgctt taaaaattat tattgaccgt gaaaaagaga
    43561 tccgagaatt tgttccagaa gaatattgga gcatcgacgg taattttaaa aaagctcgca
    43621 agaaattcaa agcaaatttc tggggaatcg acggtaagaa aaagaaatta ccagatgcac
    43681 aaagtgtaaa agaagtcact gctagaattg atggaacaga cttcgaagtt aaaaaagtag
    43741 aaaagaaaga gcgcaaaaga aaccctgcga atcctttcac tacaagtagc ctgcaacaag
    43801 aagctgcccg taagttaaac tttcgaacaa gaaaaaccat gatggtagca cagcaattat
    43861 atgaaggaat cactttgggt aaacaaggaa cagtcggctt gattacatac atgcgtactg
    43921 attctacccg gattgccgat acagcaaaag cagaggccgc agcatttatt gaagatacgt
    43981 atggaaaaga attttctaga catgatcacc gaaagatcaa aaatgctcaa ggcgcacaag
    44041 atgcccatga agctgtgcgt ccaacgagtg tgcttcgtac accagatgaa ctcaaacaat
    44101 atttagataa agatcaattg aaactttata cattgatctg gtcacgtttt gtggctagtc
    44161 agatgacacc agctatttta gatacaatga aagtgacatt agaacaaaat ggtgtcatct
    44221 tcattgcgaa cggttcaaaa atcaaattca aaggattcat gcaagtgtat gtggaaggta
    44281 gagatgacgg caaagaagag aaagaaaata ttcttccaga acttgaagaa ggcgatgtag
    44341 tccaatctgt tgatattgaa ccaaaacaac attttactca accaccagca cgttttagtg
    44401 aggctacgtt gattcgtgca ctagaagaaa acggggtagg gcgaccttcc acttatgcac
    44461 caacacttga tacgatccaa agacgctact atgtaaaatt gacacaaaaa agatttgagc
    44521 cgactgaact aggagaaatt gtcaattcat taatctgtga attcttccct caaatcgtcg
    44581 atatccattt cacagcagaa atggaaggag atttggataa aattgaagaa ggcacggaag
    44641 cgtgggtcaa agtggttgat cgtttctata aaccgtttga aaaagaactg acgaatgcgg
    44701 aagaaaaaat cgaaaaaatt caaatcaaag atgaaccagc aggattcgat tgtgatgtct
    44761 gtggacatcc gatggtgata aaattaggga aatatgggaa gttttatgct tgcagtaatt
    44821 ttcctgattg tcggaatacg aaaccaatcg taaaagaaat cggtgtgact tgcccagtat
    44881 gtcatgaagg acaagtcatt gaacgaaaat ctaagaaaaa cagactattc tatggttgtt
    44941 cccgtttccc agaatgtgaa ttcacttctt gggataaacc agtgggcaga aattgtccaa
    45001 aatgtgacgg atatctggtc gagaagaaag tcaaaggcgg taaacaagta gtctgtgtaa
    45061 atggagatta cgaagaagac atccaaaaat aatagaaaag aggttctttc attgaaaatg
    45121 gtaaatgttg ttggagcagg gcttgccgga agtgaagctg cttggcagat cgcacaagca
    45181 ggagtccctg tccgtttata tgaaatgcgt ccagtcaaat cgactgaggc gcatcacaca
    45241 agtaattttg ctgaactagt atgttccaat tcattaagag gaaacagttt ggctaatgca
    45301 gtagggttat tgaaagaaga aatgcgccga ttgaattcgg tagtaatcac ttcggcagat
    45361 gaaacagcag tgccagctgg tggtgcctta gctgttgacc gcgattcttt ttctgaaaca
    45421 attacccgaa aagtcaaaga gcatccatta gtaacagtga taaacgaaga aattactgaa
    45481 ataccggaag gaatcaccgt agtagcaaca ggacctttga cttctcatcc attagcagaa
    45541 agcatcaaag cattcaatgg ttcagatggt ttttatttct atgatgcagc tgcaccaatc
    45601 gtagacaaag ctacgatcga tatggataaa gtatatttga agtcccgcta tgacaaagga
    45661 gaagcagctt acttgaactg tccgatgaca gaagaggaat tcaaagcttt ccatgaggca
    45721 ctaatcaatg ccgaagtggc agaacttaaa tcttttgaga aagaaaaata ttttgaagga
    45781 tgtatgccaa ttgaagtaat ggcacagcga ggattcaaaa cgatgctttt cggacctatg
    45841 aaaccagttg gattggaaga tcctaaaact ggcaaacgtc cttatgctgt tatccagctg
    45901 cgtcaagaca atgctgcagc ttctttatac aacatcgtgg gctttcagac tcacttgaaa
    45961 tggggcgagc aaaaacgtgt tttccggatg attcctgggt tagaaaatgc tgaattcgtt
    46021 cgctatggtg tgatgcaccg aaacagcttt atgaattcgc cagaattgct gaaaccgact
    46081 taccaatcaa cgaaacgcga agatctgttc tttgcaggtc agatgactgg tgtagaaggc
    46141 tatgttgaaa gtgcagcgag cggactactt gctggaatca acgccgcacg cttagcaaaa
    46201 gaaatggaac cagtcgagtt tccacaagaa acagctattg gaagtatggc ttactacatt
    46261 actcatgcgg aaggaaaaca tttccaaccg atgaatgcca actatggtct gtttcctgaa
    46321 ctgccagagc gcattcgaga caaaaaagca agatatgaag caattgcaca acgagcttta
    46381 aaagctaatg aggcaataaa aacagaatta gaaaaaactg caatcccaaa tgagtaagga
    46441 aactccatga agagggtgag acggatagtc tcgctctttt tcttgcgttt ttcttaagaa
    46501 tttctcaaac aaaatgtgag gaattgcgcc aattgtgaac aaacttagaa atattggtta
    46561 aattctgaca atttatttgt ctttttataa acgctgtgct aaattattct ctgggacaaa
    46621 aggaaggtga gaaaatgaca gaagccacgt gggaacaaaa atttttgcgt tatttgattg
    46681 tcgaaagagg atattcagaa aaaacgaaag aagcatatga agaagacatt ctccatttca
    46741 aacatttttt ggaggaatct ggagacgctg atctgctgaa ggcggaatat tttacggttc
    46801 gtgtctattt gagtgcatta gctgatcatc gtcctagcta tagcagaaat tctatcagtc
    46861 gcaagatagc cagcctacgt tcgttttatc agtatcttct caaagaagaa gtgataaaag
    46921 aaaatccatt ttcttacgtt catctcaaaa agaaaaattt gagactgcca cgtttttttt
    46981 atgaaaatga aatgcaagca ctttttgaca gcgtagcagg agatagtcct ttggatttga
    47041 ggaaccgcgc tttactggaa gtcctttacg ggtcaggcat ccgtttaagt gagtgtagca
    47101 gtttgtcggt atcagacatt gattttgatt cagaggtcat gttgatccat ggtaaaggga
    47161 acaaagagag atatgctcct ctaggttctt ttgctcaaga tgctctgcaa gaatatttca
    47221 gggaaggccg ccaggtattg atgacaaaat atcacgaaga gcatgatatc gtttttgtga
    47281 atcatcatgg aaaaccaatc acgcctactg gaattgaata tgtcttgaat caaatgatca
    47341 aaaaaagcag tttggatagt gatatccatc ctcacatgct gcgccataca tttgctactc
    47401 atttattaaa taatggggca gatttgcgga cagtccaaga attgctagga catgccaatt
    47461 tatcgactac ccagatctat gcccatgtaa caaaagaaag tttacaaaaa aactatcgtt
    47521 ctttccatcc aagagcgtaa aaataaggaa aagaacaaat ttaggaggaa acaaacatgg
    47581 tcgaatcacc atttcattca accactatct gtgccgtcga aaaagacggg aaatttgcta
    47641 tggctggaga tggccaagta acaatgggtg aatctgttgt catgaaaggc actgcaaaga
    47701 aagtacgacg tatctacaat ggagaagttg ttgtaggttt cgccggaagt gttgcagatg
    47761 cgtttacttt agaagaaaaa tttgaaggca aactaaacga atataacggg aatctacaac
    47821 gcgcagctgt cgaattagct caagaatggc ggacacagca atctatgcaa aaacttgaag
    47881 cgatgctgat cgtaatgaat gataaagaaa tgcttcttgt ttcaggtaca ggagaagtta
    47941 tcacaccaga tgatggtatc ttagctatcg gatcaggagg gaattttgcg ttggctgcag
    48001 cccgagcaat gaaaaactat gctcaaaacg acatgagtgc gaaagaaatt gcagaaaatg
    48061 cgttaaatat tgccgcagat atctgtatct ttactaatca taatattatc gtagaagaac
    48121 tgtaagaggt gagtagatat gtatgaatta aaacaaacac caagacaaat cgtaagtgaa
    48181 ttagatgaat atatcgtagg acagcacgca gcaaaaaaat ctgttgccgt tgctttgcgt
    48241 aaccgctatc gccgtttgca gttagatgaa aaaatgcaac aagatattac accaaaaaat
    48301 atgctgatga tcggacctac aggggtcggt aaaacagaaa tcgcacgacg tttggcaaaa
    48361 atcgtcaatg caccatttgt caaagtcgaa gcaacaaaat ttactgaagt cggttatgtc
    48421 ggaagagatg tagaatcaat ggttcgggat ttagtagaaa atgcgatcca aatcgtagaa
    48481 aaagagcagt acagccgtgt atacacaagt gctaaaaaaa atgcagaaaa acgtttggta
    48541 aaaatcttgg tacctggtat caaaaaagaa cagaaacaat ccactaatca atttgaacaa
    48601 atgatgaatc tttttaatgc tagtcagcaa aataccgctg aaccgcaaga agaagtgaca
    48661 gaagatattc gaatcaatcg ccgtacagtc ttggaacaac tacaaaaggg tttactggat
    48721 agtcgtgaag taaccattga agttgaagac caaaagaaac ctgcaccaat gatgaacaat
    48781 ggattggagc aaatggggat cgacctaaat gaaactctag gtgctttgac accgaagaaa
    48841 aaagtggaac ggacggtaac agtcaaagaa gctttagagt tattgatcaa ggaagaatca
    48901 gcaaaattag tcaaggatgc tgatatccat agtgaggcaa tcaaattagc ggaagaaagt
    48961 ggaattattt ttatcgatga aatcgataag atcacttcta aatcacagca atctggagaa
    49021 gtatccagag aaggcgtcca aagagacata ttgccaattg tcgaaggttc acaagtaaac
    49081 acaaaatacg ggatcatcca aacagatcac gtcctattca ttgcttcagg agctttccat
    49141 ttgtctaaac caagtgatct aattccagaa cttcaaggac gcttcccgat acgggtagaa
    49201 ttagatgatt tgacagctga agattttgtg aagattctga ccgaaccaaa taatgcttta
    49261 gtaaaacaat acgtcgcgtt gatcggtaca gaaaatgtgt cagttacgtt tacaaaagaa
    49321 gcaattgaac ggatcgctca aatcgctttc gatgtcaacc gtgacacaga taatatcggc
    49381 gcacgcagac tgcataccat tttggaaaaa ctactagaag atctattatt tgaagcacca
    49441 gatatgcaaa tgggagaaat aacgatcaca gaagcatatg tggatgacaa acttggctca
    49501 attgcggcaa atgaagattt aagtcgctat attttataaa aaatcggagg acgacaatga
    49561 cagacttgct aacgaaaacc cgcaagataa acacactttt gcagcaaaag aatacttttg
    49621 acatgcaagc agatttgcct tacgataaaa tggctgtcac actaggtggt attcttgaca
    49681 gcaacaccta tatcatagga agtgacggag tattactagg ttataatgaa cgtcacgatg
    49741 tgaacaatga acgaatcaaa acaatgtttg ttgagaaaca atttcctgaa tcttatactg
    49801 atacagtggc acagttgatg aaaactgaag ctaatatccc aattactagt gatattactg
    49861 catttccggt agagtcaaga gagaaatatc catttggtct gacgactgtc gttccgatct
    49921 ttggagctgg cgaacgtttg ggtacgatta tcttatctcg tatggaacaa tcgtttgcag
    49981 atgacgatct agtcttagca gaatatggag ctacggttgt cggtatgcag atcctttatc
    50041 agaagtctcg aagcatcgaa gcagatgtac ggagtgcaac ggctgtccaa atggcaatca
    50101 acacattatc ttatagcgaa ttgaaggctg tacaagctat ctttgaagca ttggatggag
    50161 atgaaggtcg tctgactgct tctaatattg ccgatagtat cggcatcact cgttctgtga
    50221 tcgtcaatgc tttacgaaaa ttggaatcag ctggaatcat tgaatctcgt tcgttaggca
    50281 tgaagggaac ttatttaaaa gtattgaatt cccgttttaa agacgagtta gcaaaacaca
    50341 gttattagaa gtaaggggac tggggcagat agaaatgttt gttccagcct tcccatttta
    50401 aaaagtaagt cgaagttgga ggaatggcaa tgactgttat tattcaaaat gaccagctga
    50461 tcgccgaaat cgctgaacac ggtgcggaat tgatcagtct gaaatcaaaa gaacatggtt
    50521 tagagtatat ctggcaaggt gatccagcgt attggggcag acatgcaccg gttttattcc
    50581 cttttgttgg acgtttaaaa gatgatcagt atgtttatca agggaaaaca tatcaaatgg
    50641 ggcaacacgg atttgctcgg gatatggatt ttgaagtcat tgaacatgga caagaaatgg
    50701 ctagtttcat cttaaggagt acgccggaga cattagaaaa gtatccattt gattttgaat
    50761 tagtgatcag ctatgaatta ggaggagacg gcatcacggt tcattatcaa gtggcaaata
    50821 ccggtgaaaa agaaatgttc ttttctatcg gtggacatcc tgctttcaat gtacctcttg
    50881 aagaaggatt gtcatttgaa gatttctatc tggcattttc tccattgaaa tcaagattac
    50941 gcctaccatt gaaaggccca ttcatcgata tggatcaaaa gacgttaggt cagacaaata
    51001 cgaatctggc tttgaatcgt gaactttttg agcaagatgc cttggttttt gaaacaaggg
    51061 gtctaaatgc gtacgccatt cgaagtgaaa aatcttccca tagcgtaact ttaagctata
    51121 aagatatgcc ttatgtaggg atctggtctc cttatccaaa agaagcgcct ttcgtttgta
    51181 tcgaaccgtg gtgcggaatc gctgacacgg tgaacagtag tggaaacttg atggaaaaag
    51241 tagggatcaa tcaactaggt gcccatgaaa tcttcaagac aaaatattcc atcacagtga
    51301 agtaagaggt tgtgaccgag aagttagcag ggaacaccgt ttaccaaggt gtgtaaaaaa
    51361 gctatcctgt aagtaataag ggagtgtgac atcgatttgt cacactcccg ttttatctct
    51421 ttggaatgaa aaagtatcag gattttttct tgctgctatt caaaccaaac gggattcgac
    51481 tttcagtatg gtttctgata cgcttgatat tgtctttatg ccgataaaag ataaaggaag
    51541 aaactaaaaa ggcaatgatc gtcagtaacc aattgggctt agccaaaatc acaggagcta
    51601 cataaggaag gacgatcgta gataaagtga tcaatactgc actgatcata ctgctcaaac
    51661 tcaccatact tgtacaaaat aaactaacga taaagatgat acaagaataa acaaaaaata
    51721 caggattaaa tcctaaaagc attcctgcac ttgtcgcaac agcttttcct cctttgaacc
    51781 cagcaaaaat cggaaatgta tggccaagaa tggcgcatac cccaaaccaa agagggttaa
    51841 tcccagaaag gtggaagatc atcggcaaac aagtagccaa tgtacctttt aaaatatcca
    51901 taagcaggac aacaatcccg gctgttttgc ctaatacacg aaatgtattg gtcgttccag
    51961 tattaccgct cccgtgttgt cggatatctt ttttgaaaaa caatttgcca atccagacac
    52021 cggaaggaat agaacctaaa agatatgcta aaataagtaa taagatcaat ttcatttttt
    52081 accctctttc aagttgaaaa ctatccttta ttttagcatg aataggtaac aggaacaatt
    52141 gaaaaaatga aagatacaag tataatagat ggaataaaaa gaaaagcaag gggtggcaga
    52201 aatgtctgta aaaaatccaa cacaagaaac gattttcaac tatttggaag aagcaaaaaa
    52261 tattgccgtc gtaggcttga gcaataaacc agaacgaaca agttacatgg ttgcacaggt
    52321 attgcaagac aatggatata aaatcatacc agtaaatcca ttattggctg gtgaagaaat
    52381 tctaggcgag aaagtctatg cgaatttgac agaaatcgat gatccaatcg atatcgtaga
    52441 tgtatttaga cggagtgaat ttttacctga aatagcaggt gattttttga aaactgatgc
    52501 taaagtgttc tgggcacaat tagggattga aaacaaagaa gcagctgaga tgctaagaaa
    52561 agcaggacgt acagatatcg taatggatcg ttgtattaag attgaacttg gtaagttgaa
    52621 agaaaaccaa aaataaaaat gaccacaaag aagagatgat cgatagaggg gaatgtcgaa
    52681 aaagcggtta ggagataatt aaaattgatt tctcgtctac cctctttttt ttcacgacct
    52741 tttctagtat gataatcgtg gtgcggtttt gccgcaaagt attattgaag gagcgtttgc
    52801 cttggcaaaa aaagtaaaca acgaatacaa tgactcgtcc atccaagtat tggaaggatt
    52861 agaggcagtt agaaaaagac cagggatgta cattggatcc actgacagcc gaggcttgca
    52921 tcatttagtc tatgaaatcg tcgataatgc agttgacgaa gcattatcag gttatggcag
    52981 tgagattgat gtgaccatcc atgaagacaa tagtataact gttgcagata gcggacgtgg
    53041 gatgcctgta ggaatgcatg cttccggaat accaacagtt gaagtgattt ttactgtgct
    53101 gcatgctggc ggaaagtttg gtcaaggagg gtacaaaact tctggtggac tgcacggtgt
    53161 aggagccagt gtggtaaatg ccttatcgaa atggctaatt gtaacgatcg ttagagatgg
    53221 cgtagagtat cagcagaaat ttaaaaacgg cggtaaacct gacggtactt tgaaaaagat
    53281 aggaaaaaca aaaaaagcaa acgggacgac tgtccatttt ttaccagatg atacaatatt
    53341 cagtacaacg aaattttcat atgaaatttt agccgaacgg ttaagagaat ctgcctttct
    53401 tctaaa
//
LOCUS       DACECY010000227        21056 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.guided.048, whole genome shotgun sequence.
ACCESSION   DACECY010000227 DACECY010000000
VERSION     DACECY010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.guided.048"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IWO38_003000"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IWO38_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0314993.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2039..2227
                     /locus_tag="IWO38_003001"
     CDS             2039..2227
                     /locus_tag="IWO38_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0314994.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2285..2638)
                     /locus_tag="IWO38_003002"
     CDS             complement(2285..2638)
                     /locus_tag="IWO38_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0314995.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            3169..3591
                     /locus_tag="IWO38_003003"
     CDS             3169..3591
                     /locus_tag="IWO38_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0314996.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            3588..3818
                     /locus_tag="IWO38_003004"
     CDS             3588..3818
                     /locus_tag="IWO38_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0314997.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            4316..4516
                     /locus_tag="IWO38_003005"
     CDS             4316..4516
                     /locus_tag="IWO38_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0314998.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            4543..5736
                     /locus_tag="IWO38_003006"
     CDS             4543..5736
                     /locus_tag="IWO38_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0314999.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(5804..7369)
                     /gene="guaA"
                     /locus_tag="IWO38_003007"
     CDS             complement(5804..7369)
                     /gene="guaA"
                     /locus_tag="IWO38_003007"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ0315000.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(7428..8351)
                     /locus_tag="IWO38_003008"
     CDS             complement(7428..8351)
                     /locus_tag="IWO38_003008"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ0315001.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            8547..9152
                     /locus_tag="IWO38_003009"
     CDS             8547..9152
                     /locus_tag="IWO38_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0315002.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            9460..10476
                     /locus_tag="IWO38_003010"
     CDS             9460..10476
                     /locus_tag="IWO38_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0315003.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            10617..11918
                     /locus_tag="IWO38_003011"
     CDS             10617..11918
                     /locus_tag="IWO38_003011"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ0315004.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            11964..12626
                     /gene="deoC"
                     /locus_tag="IWO38_003012"
     CDS             11964..12626
                     /gene="deoC"
                     /locus_tag="IWO38_003012"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ0315005.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            12647..13045
                     /locus_tag="IWO38_003013"
     CDS             12647..13045
                     /locus_tag="IWO38_003013"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ0315006.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            13111..14193
                     /locus_tag="IWO38_003014"
     CDS             13111..14193
                     /locus_tag="IWO38_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0315007.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            14405..15967
                     /locus_tag="IWO38_003015"
     CDS             14405..15967
                     /locus_tag="IWO38_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0315008.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            15960..17093
                     /locus_tag="IWO38_003016"
     CDS             15960..17093
                     /locus_tag="IWO38_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0315009.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            17093..18049
                     /locus_tag="IWO38_003017"
     CDS             17093..18049
                     /locus_tag="IWO38_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0315010.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            18148..19314
                     /gene="deoB"
                     /locus_tag="IWO38_003018"
     CDS             18148..19314
                     /gene="deoB"
                     /locus_tag="IWO38_003018"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ0315011.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            19338..20156
                     /locus_tag="IWO38_003019"
     CDS             19338..20156
                     /locus_tag="IWO38_003019"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0315012.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            20173..20880
                     /gene="deoD"
                     /locus_tag="IWO38_003020"
     CDS             20173..20880
                     /gene="deoD"
                     /locus_tag="IWO38_003020"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0315013.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         6778 a         3760 c         4523 g         5995 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2221 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2281 ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg
     2341 atagttcttt gtctgtaaag ctatccatgt atttttctac ctgtaacctt cgagtgcttt
     2401 taatcaaatt atctgtaggt aagaaaaatt catcaataga tacatggagt aatgatacaa
     2461 gatcatacag cacttgtgta cttgggtgtt gtcctttgtt ttctatattt gttaaataac
     2521 gtgggtcaat ttcaatcatt gttcccactt gttcacgagt gagaccccgt ttcattcgag
     2581 cctctttaat cgctaaacca acggctctaa aatcgtattt atcttctttt ttacgcatac
     2641 taaatcacct ctatacattt tactgttcct atataaccat aaacaggtat agaaaaacat
     2701 gttatatagt ttatgagttc ctatagaaca ctgtggaatt tattaatgcg atgaatccaa
     2761 tttcaaatag aaaggtcgct gattttattt acctatacaa ctttataaaa tttagtcata
     2821 ctcctagaaa gtagataaaa ttggcgactt aatggaggga aaagtcgcca tgagaaagat
     2881 aatctgtcat ctagttctct taattaagtt cttatcaaaa aaacttatcc acctgtactg
     2941 gatagatatg accatcaatg tggttttgca gattacatag atagaaaaat caaagcatat
     3001 gaacagagaa aacaatctgt ttatgtgctt ttttggtcac ttaataaata tcttttagga
     3061 tttctcttca tttatgcaac aaatcccccg ttcgtgtcgg ggtttagtat gaagggagat
     3121 aaagaagcac atgccactcc ctttcacgac agaaaggggg tgaaaagcat gaaaccatct
     3181 tcttttcaag aaaccataga aaatcagttt gactatatct gcaaacaagt tattgaagat
     3241 gaacgaaaag attacttcaa acatctagac agactatcag caaaagaagt ctcgttttct
     3301 gatatagata actaccttgt caactctttt tctactgttg atacgtacgt cactgatttc
     3361 caactattta aattatttgg attaagaatc aatgttgaaa gtgatttatt aagtgaagcc
     3421 ctgcgtcatc tttcagagaa aaaacggaac attattttac ttcattactt tatggatatg
     3481 agtgatgtgg agattgcgga gttactaaat ttgaatcgtt cgactgttta tcgtcatcga
     3541 ataagtggac tagccatgat taaagaattc atgaaggagt gtaaagaatg aaaacagctt
     3601 atccaagggt tcccttcccc ctcattgtaa aggcgacaga tggcgacgtg gaagcaatta
     3661 atcagattgt taaacattat cgagggtata cctcaaaacg ttcattacgt cgcatgacag
     3721 acgaatatgg aaattctcat atggttattg atgaaaccct acgtggcaga atggaaacga
     3781 aactcatctc taagatttta tcgttcgaaa ttaaatagtc ccattttttc gtggaagcgt
     3841 gtcgaatcgt atccacgctt cccgatttgg gaggtttgtt attccatcaa agcatattgt
     3901 gtgttcagta ggttttgata ggctaacaag ccaaacgttc tttgacaacc aaataaaggc
     3961 aatcgaatac gtttcggtaa gaaaagagct gatgaactaa cacgccatga cctacaaaat
     4021 tagtgagcga ttcatgttag taattcgaaa gtaatcttta gcaggattgc tgccatgact
     4081 ttcttatcgt gataatgata ctcccgtata gccatagtcc gagcgttaga agcgtcacag
     4141 gcaatgggta cggctacatg agaaccatgc agaagtgaaa ttcctgtgag ctttgctaga
     4201 gctgttcgat tgctaagata ttcttctctt gtcagtgtat tattataaaa ctatttgata
     4261 taattatagg tgaatattgg agttttccta gcctataatt gggaaaggag taataatgat
     4321 aacggtcaca aaaaaggatt tagttgagct aggatatgga acttcttttt cagcagacat
     4381 cataaaaaaa gcaaaagaat tgatgattac aaaagggcat atctactatc aatctcgaaa
     4441 gctagataga gtccctcgtg aagctgttga agaaattcta ggaattcggt tttcaaatga
     4501 aaacaggtct gagtagtttt tgcacagtaa ggagtaaaaa ctatggttaa agacccaata
     4561 aaaaaagcaa agaatggaac ctattatttt cgtgcaaatc taggttatga ttctaaaggg
     4621 aaaaaaattc aaaaatatcg tagtggattc actactaaaa aagaagcaag agaagaatat
     4681 tcgaaactgc tattgatgaa accagaagaa ttaagtgaga ataaggataa aatgacgttt
     4741 aaacattata tctttgaaat tttccttcca tggtataaga cacaggtaaa gctcagaaca
     4801 tatgaaaatc gcttaccaac tattaagaag catttctctt attttgataa aatggctgtt
     4861 tccgatattg agcctattga tgtgcaaaat tggcaactaa aattatcaaa gaagtgtaag
     4921 tcttcttatg taagagctgt tcaaggacta ttttcagttg ctatggatcg agcaattgtc
     4981 cttgggataa ccaccacaaa tccatctaag attattggca atgtgaaaaa gcaaaagtct
     5041 aaaattgaat tttggacaaa agaagaattt gaaaaagtaa tctctctaat ttacaaagaa
     5101 gattactatc aacacttttt atttatctct ctgtggtttt tatttatgac tggaatgcga
     5161 attggtgaag ctactgccat tcaatgggaa gatattgatt ttgactcagg agtactaacc
     5221 atcaacaaaa cactctatta caagaatcaa tcgaattata gatttgttga accaaaaaca
     5281 aaggcgagcg ttcgtcatat tgtattagat aaatgcacat taacttattt atccgaatgg
     5341 aaggaagtac aacaaaatct aattcagaca gactttgtaa tgagctataa cggtattcct
     5401 acccaaaaac atacactatc atatgctatt gaacgctatg ctaagctggc tggtatccat
     5461 cgaatcagaa tccatgggtt acggcattct catgcttcct tgttaatcag tatgggagaa
     5521 aatccactta tcattaaaga tcgtctagga cacgaagata ttgagacgac tttaggaacg
     5581 tatggacatc tttatcctaa cagtaatttc gaggtagcta acaaattaaa tggagccatt
     5641 tcctttaaag aagcaaaaca aaattatgat acttcaccaa agaatcaatt tactgtagac
     5701 tacttgcgaa acaaagaaat gaaaaaagtg caataaaagt gcagtaaaaa gataagaaga
     5761 gccagaaacc cttggtataa gaggatttct agctctccta caactattcc cactccactg
     5821 ttgctggtgg tttagaagtg atgtcataca cgatacggtt gacgtgggct acttcgttta
     5881 cgatacgtac agagattttt tgcaagactt cccatgggat acgggcaaag tcagcggtca
     5941 ttccgtcgat tgaagttaca gcacggatac cgactgtgta gtcgtaagta cgtccgtcac
     6001 ccataacacc gacagagcgg atccctggaa gaacagtgaa atattgccag atatcacggt
     6061 caagtccagc agctgcgatt tcttcacgca agatagcatc agattcgcgt acgatttcta
     6121 gcttgtcttc agtgatttcg ccaagaacac gaataccaag accaggacca gggaatggct
     6181 gacgccaaac gattgaatca ggcatgccta actgagtccc taattcacgg acttcgtctt
     6241 tgaataatgt gtttaatggt tcgatcaatt caaattgcat atcttctggc agtccaccta
     6301 cattatggtg agatttgatc gtctgtgctg tttctgtacc agattcgata acgtcagtat
     6361 acaacgttcc ttgtgctagg aaagagacgc cttcttcacc agcaagtttt gttgcttcgt
     6421 catcaaaaac atatacgaat tcattcccaa tgattttacg tttttgttct gggtcagata
     6481 cacctttcaa cttgtctaag aagcgatctc ttgcatcgac tttgatgatg ttcaagccga
     6541 attttccgcc tagactttcc attacttgtt ccgcttcgcc tttgcgtagc agaccgtggt
     6601 caacaaagat agatgtcaat tggtcaccga tcgctttttg taaaagaacc ccaacgacac
     6661 tagaatcaac gccacctgaa agaccaagca agactttttt gtcaccaact tgttcacgga
     6721 ttttttctac ttgcatatcg ataaagttat ccattgtcca gttaccttca cagtgacaaa
     6781 catcaaaggc aaagtggcgc agcaattcga ttccgtattc ggaatgacgt acttctgcat
     6841 ggaattggat accataaaaa ttacgtgttg tgtcttggat agaagcaatc ggacaatctt
     6901 tgcttgttcc gactgtttca aagccttcag gaactttagt taccaagtca ccatggctca
     6961 tccatactgt ttgttgtttt ggtgtttctt taaacaagac agcttgatcg cttgttactt
     7021 ctaacaaggc tttcccatat tcgcggtttt tagcaggctc tacggaaccg ccaagtttgt
     7081 acgtgatcaa ttgcatacca tagcaaatcc ctaatacagg aatacctaaa ttgaagatct
     7141 caggatcgat cccaaatgca ttctcatcat atacactgtt tggtccgcca gaaagaatga
     7201 ttccctttgg attcatcgct tttacttctt ctgcagtaat gcggtggctc aataattcgg
     7261 aaaagactcc aagatcacga atacgtcttg tgatcaattg gttatactga ctaccgtagt
     7321 ctaatacgat aatcttttca acgttcgtca tatcggcaac gtttgtcaaa tttattaccc
     7381 ctatcccttt aaattttatc tcaaaatagt caaactattt taaaatgcta atattttcgc
     7441 atgaaaatcg aatcgatgat atgattttca gttttatgga gaattatatc ggctcttcct
     7501 cgtgtaggca agatatattc ctgtaaattt ttcaaattca catttttcca tacatctttt
     7561 gccattttaa aggcttcttc acgatctccg atagcaaaac gataataata attctctgga
     7621 ttttggaaag ccgtatccag taacgcacca aaacgatcca aataccattt ttcgatcagc
     7681 acgggatcgg catcgacata gacagaaaaa tcaaaaaagt cgctgacgta gatttgctga
     7741 ttcgcaggta attgaagcgt attgatcccc tcaacgatca agatatcagg ctgctggatc
     7801 aattcgtact tgtctggaat gatatcatac acatcatgtg agtaaacagg tacttttatc
     7861 tcttcctgac cagattttac acggttcaag aaatcgatca gcatctccat atcataactt
     7921 tcaggaaatc ctttacggtc catgattcca cgctctttta atatacgatt tggataaaga
     7981 aatccgtcag tcgtgatcag ctgaacgttt cttcgtttga aggtacgaga caaaatcgtc
     8041 tgcagcagtc tggctgttgt actttttcca acggctacac ttccggcgat cccgataata
     8101 aaaggcggta cttctacgta tttatgaaga aataaacctt tactcaagaa caaagattca
     8161 aattctttca tataaagata aatcagatga gcaagaggta catagatatc tcgaacatcc
     8221 tgcatggaaa tgcggtcatt caaacttttg atattatcta gttcatcttg tgtcaaaggt
     8281 gcttgcccat tacgataaaa ttctcgccat tcttctcttg ccacacggta gtagttcatt
     8341 ggatcattca tattagcctc ctgcgttctc ccatcaatgg aactatttta acacaaaata
     8401 aacgcaaacc ccaccactta agagagattg ccgatctttt tgtataatct ttataataga
     8461 aagaaacgga gtcttattct tgacttgcgc acggatacat aaaaggatag actgttgtca
     8521 tgaaagaaat gagaaggatg gaagacatga caaatcatta ctattctgaa cgcccagata
     8581 cggcgcacga ttttgaacaa tgggcttttg agttaaaagg cagaaccttt caatttgtaa
     8641 cagattctgg tgtattttca cgtgaaacag tggattatgg ttcacgtgtc ctgattgatg
     8701 ctttcgattg ggaagagtta cctgatgaag gaaagatctt agatgtggga tgcgggtatg
     8761 gaccgattgg gttagctttg gcatttgcga cacagcggtt tgttgaaatg gtcgacatta
     8821 atgaacgagc ggtaagtcta gcgcaaggaa acgcaaagcg taaccaaatc gaacatgttg
     8881 atatccatca atcgaatatc tatgaagcag ttcatgaaga aacctatgca gctatcgtaa
     8941 gcaatccgcc gatccgtgct ggaaaaaaag ttgtacatga aattttgaca ggcgcatatc
     9001 ctcggttgaa gaagggcggt acactgacaa ttgtgatcca gaagaaacaa ggagcgccga
     9061 gtgcgcaaaa gaaaatggaa gaaacatttg gtaatgcaga aatcgtcaca aaagataaag
     9121 gatattatat catcaaaagt gtgaaagaat aactagctga aaaatagtgg agcgacgaaa
     9181 gagtggaact gggcgtgcat gtacgggtac gtatcagttc tttttttgtt gagaagtttt
     9241 taaaaacaaa gaactatttt ttcttcaaaa agaaatagta ggaacagagt cgttgttttt
     9301 tacgataaaa taaataagat acagaacttg tatagaaggc tgccaagtcg gtagataaag
     9361 gaggttttta tcaaaaaaca tactttcctt ttttctctat tcttttcttg ggggttttgg
     9421 tacactgata gggaatttag atagaaggga gccaatagga tgaagctgac catcagagat
     9481 atcgcagaaa tggcaggcgt atcagtaaca actgtctcac agattttgaa taacaaagga
     9541 agcagattta gtgataagac aagaaaaaaa gtgttgtcga tcgtgaatga acatcattat
     9601 aagcccgatt atttcgcttc aaatgtgatc aatcgacatt caaagaccat cggaatgatc
     9661 gtccctgatg tcactgattt cttcttctcc aaactgatcg aaggtgtaga gagctatttg
     9721 aatcctttgg gctatgtgat catgttatgc aattctcgtc atagccaaga aaatgagatc
     9781 aagtatttac aagagctttc ccaccgctca gtggacggaa tcttgctggc cacaccaaat
     9841 attttacctg agaaatatgc attagacagc ggtttttatc aaaaaatgcc aatcatttta
     9901 atcgatcgcg gattgaatcg tcgggataac ggacggctta tcgtaaagga atatgagggc
     9961 gcctatcaag ctgtttcgct gtttattgaa aatggccata cgaagatcgg catgctgaag
    10021 gaaaccactg gatactatca attagaagaa cgtttcaacg gatatcgtca tgcgctgaaa
    10081 gatcatggcc tgccttttaa tgggaaattt gttgaacatg gagagttgac tgtccaaggt
    10141 ggatatactg catcaaaaaa attactcaaa cataaagata tcacggctat tttttgcggg
    10201 aatgatgcca tggcgatcgg atgctatcag gcgatcgatg aattgggcaa aaaaatccca
    10261 gaagatatct cagtcatagg attcgatgga ttaaagctgt cagaatacat gattccgcaa
    10321 ttgacgacag tgcagcagcc aagttttgat attggatttt atgctgcacg tttcctgatc
    10381 gatacgatcg aatttcctca aagaaaagtt ccgaacaaag tgttcgaaac aaaattaatt
    10441 attcgtgaga gcgtaaaagt attgacaaag ggctaactcg gcggtatatt gcttatgtaa
    10501 gcatttacaa aggctagtta gtgctttttt tatttttagt aaaccagttt actaaagtgg
    10561 tttacctttc ctaaaaataa tgtataatac cgaatgaaga caaggaggat caggcaatgc
    10621 gaatggtgga tctgattgaa aaaaagaggg atggaaacga gctgtcgaaa gaggaaatcg
    10681 agtacatcgt cacgaactat acgaatggaa agattccaga ttatcaagtc agtgcattat
    10741 tgatggcaat tttttatcaa gacatgacaa atgaagaaat cacgaactta acattggcga
    10801 ttgctaattc tggtgatgtc atcgatttga gttctcttga aggaataaag gtagataagc
    10861 attcgacagg tggcgttggt gacacgacca cattgatttt agccccccta gtagctagtg
    10921 tcggagtgac agtggctaaa atgtctggac gcggtctagg ttacacaggt ggtacactag
    10981 acaagctaga agcaattccc ggatttcaga tcgagctttc ggatgaagaa tttgtacgca
    11041 ttgttaatga aagtaaagta gcggtaattg gtcaatcagg aaacttggca ccggcggaca
    11101 aaaaattgta tgccttgcgt gacgtcacag cgaccgttga ttctcttccg ctgattgcca
    11161 gttcgattat gagtaaaaaa atcgcggcag gagcagatgc aatcgttttg gatgtgacca
    11221 ctggagacgg tgctttcatg aagaacatcg aagatgcaag acgtctggca aagaccatga
    11281 cgagcattgg gaaactggct aaccgagaaa cagtggcggt tatctcagat atgtcagaac
    11341 ctcttggcga agctatcgga aatagtctag aagttgtcga ggcaatcgaa acattacaag
    11401 gaaatggtcc agaagatcta gtagaaatgt gctacgcctt aggaagccaa atggttgttc
    11461 ttgctggcaa agctaagaca accgatgaag caagaacgtt gcttcaagaa gctctggaat
    11521 caggtaaagc gttagcaaaa ttcaaagaaa tgatccaaaa tcaaggggga gacccagcga
    11581 tcgtggaaca tccagaacga attttgactg cacgctacac aatggagctt cctgctaaac
    11641 aaagcggagt tgtgtcaaaa atcgtggcaa atgaattagg aattgctgca atgatgctag
    11701 gtgctgggcg taaaaccaaa gaagaagata ttgatcacgc tgttggattg aaactgcata
    11761 aaaaaattgg tgacactgtg acaaaaggtg agtcactatt gacgatctat agtaacgacg
    11821 aagaaatcac ttctgtcatt gaattattat ataaaaatat tgaaattggt gaatcggcaa
    11881 tgaagccaac gttgattcat gacatcatca cagaataaag gaaaaaagcg gactttaccg
    11941 ttttaaagaa aaggggaaaa gaaatggaac taaaccgaat gatcgaccat actattttga
    12001 aagcagacgc atctaaagaa gctgtgatgc aaattatcga agaagcaaaa aaataccatt
    12061 tttattctgt ctgcatcaat ccagcatggg ttgcgctagc gaaagaacaa ttacaaggtg
    12121 agccagtagc cgtatgtaca gtaatcgggt ttcctctagg agcaaacaca cccgagacaa
    12181 aagcatttga aacaacagat gccatcaata acggtgcaga tgaagtcgat atggtcatca
    12241 atatcggtgc tttgaaatca aaggatgatc aacaagtcca aaaagatatc gaagcagttg
    12301 ttgaagcagc aaaagatcgt gcgcttgtca aagttattat tgaaacatcg ttattagata
    12361 gagaagaaat catccgagct tgcgaaatag caaaagctgc aggcgctgat tttgtcaaaa
    12421 catcaactgg tttctcaaca ggcggagcaa aggtagagga tgtccgtttg atgcgtgaaa
    12481 ctgtaggacc agaaatgggt gtcaaagctt ccggcggtat ccataacgaa gaagaagcaa
    12541 tggcaatgat cgaagctggt gctacaagaa tcggaacaag cgcaggagta gcaatcgtat
    12601 ctggttcaac tggtgaaggc tattaatttt ttcaaaggag aagctcatgg cagaactcaa
    12661 acaagaatgg atcaacacag ctattgaagc gttagataaa gcctatgttc cttattccca
    12721 ttttccggta ggcgcttgtc tcgtaacgga atcaggcaag atttatcaag gcatcaacat
    12781 tgagaatgca tcatttggat taacgaattg tgccgagcgc acagcttttt tcaaagcagt
    12841 atctgaagga gaaaaaacat ttacgcatct agtcgtagcg gggcacaccc ccgatcctat
    12901 ctctccttgt ggtgcatgtc gacaagtaat ggcagaattc tgttcccctg atatgccggt
    12961 tacgctagta ggagacaatg gtgtgacgaa ggcaacgacc gttcgtgcac tgctgcctta
    13021 tgcatttact gaaaaagatc tataactcat gttcattcag tcagctgact gttgaaaaat
    13081 aaaacaatta ttttaggagg ctttacagaa atgaaaaaag caaaattatt tggtttaggt
    13141 gctgtcgcac tagcagcagg gttattttta ggtgcatgcg gcaacaacgg ctctactgat
    13201 tcatccggtg gcaaaagctc atcagacaca acaactgcag cattgatcac agacacaggt
    13261 ggtgtagacg accgttcatt caaccagtca gcttgggaag gactagaaaa atgggggaaa
    13321 gatcaaggac tttcacgagg aaatgatggc ttccaatatt tccaatcttc aaatgaatca
    13381 gattacatcc caaatattga ccaagcattg aatgctggat tcaaaacgat ttttggtatt
    13441 ggttataaac taaaaccagc gatccaagaa caagcaacaa acaatacagg tacgaacttt
    13501 gttattatcg atgatgtcat cgatggatta gataacgttg tatctgcaac tttcaaagac
    13561 aacgaagctt cttatcttgc aggtgttgca gcagcttata cgactgaaac aaacgtagtc
    13621 ggctttatcg gcggtgtaaa aggtgaagta atcgatcgtt ttgatgctgg attcaaagcc
    13681 ggtgttgatg caggtgcgaa agaattagga aaagaaatca aagtattgaa tcaatatgct
    13741 ggtgacttct ctgctccaga taaaggacgt tcaattgctc aaggtatgta tgcacaaaac
    13801 gcagatatca tcttccatgc ttcaggcggt acaggtaacg gtgtattcca agaagcaaaa
    13861 tcattgaacg aatcaggcga caaaaaagtt tgggttatcg gtgttgaccg cgaccaatca
    13921 gacgaaggcg aatacacatt aaacggcgaa aagaaaaact tcacattgac atctacactg
    13981 aaagcagttg gaacagtagt agaagatttg gcacaaaaat cagcggacgg taaattccct
    14041 ggcggcgaac acactgttta tggattgaaa gaagacggtg ttggtctaac agaaggacaa
    14101 ctttctgacg aagcgaaaaa agcagttgat gaagcaaaag aaaaaatcat ttcaggtgac
    14161 gtaaaagttc cagaaactcc tgaagaaaac taaaaccatt tctatagcct tgttagacac
    14221 atgtttgaca aggctatggt ttgattagaa caagaattgt tcatttatcc aagctgcctt
    14281 gtgacagttt ggataaggga acaactccct gaagtaatgt ttgtttcaaa agaagaacgc
    14341 tcactaagcg tgaatcttag tcaatcgttg ccctagatag aaatcgttag gatgtgaaaa
    14401 aaaggtggtt caagaaaata ttgtcataga aatgcagaat attaccaaag aatttggaac
    14461 attcaaggca aatgacaaca tcaatttaca agtaaaggca ggagaaattc atgcgttgct
    14521 tggagaaaac ggtgctggca aatctacatt gatgaacgtg ctttccggat tattagagcc
    14581 gacatcaggg aaaattttga tgcgtgggaa agaagtaaag atcacaagtc cgaccaaagc
    14641 caaccaatta gggattggga tggtccatca gcactttatg cttgttgatg cctttactgt
    14701 aacagaaaac atcgtgttgg gaagcgagcc tagtcgcgca gggatgcttg accataaaaa
    14761 agcacgaaaa gagatccaaa aagtttctga acagtatgga ttgtcagtca acccagatgc
    14821 ttacgttcgt gatatttcag ttggaatgga acaacgggta gaaattttaa aaacacttta
    14881 ccgaggagca gatgtactga tttttgatga gccgacagct gtattgaccc ctcaagaaat
    14941 tgatgaatta atcgtgatca tgaaggaatt agtcaaagaa ggcaagtcga tcattttgat
    15001 tacgcataag ttagatgaaa tcaaagcagt agctgaccgc tgtacagtta tccgccgtgg
    15061 aaaaggaatc ggtacagtca acgttaaaga cgtaacctca caacaattag ccgatatgat
    15121 ggttggaaga acggtttcat tcaaaacgat gaaaaaagaa gcgaagcctc aagaagtcgt
    15181 tttgtctatt gaaaatctag tggtgaaaga aaatcgtgga ttagaagccg tgaaaaactt
    15241 gaacttagag gttcgtgctg gcgaagtact tggtatcgct ggaatcgatg gaaacgggca
    15301 gtcggagttg atccaagctt tgactggttt gcgaaaggca gaaagcggac atatcaagct
    15361 aaaaggggaa gacatcacta ataaaaaacc ccgaaaaatc actgaacatg gtgtaggaca
    15421 cgtgccagaa gaccgtcata aatacgggtt agtcctagat atgacattat ctgaaaatat
    15481 cgcactacaa acctatcatc aaaaacctta cagtaaaaac ggcatgctga attattcagt
    15541 aatgaatgaa catgccagag aattgatcga agaatatgat gttcgaacaa cgaatgaact
    15601 tgttcccgca aaagctttat caggcggaaa tcagcaaaaa gcaattatcg ctcgtgaagt
    15661 cgaccgagat cctgatctgt tgattgttgc aaatccaact cgtggactgg atgtaggtgc
    15721 gatcgaattt attcataaac gtctgatcga acaaagggac aaatacaaag cagtgttact
    15781 gatcagtttc gaattagaag aaattttaaa tgtttcggat cgtattgctg ttatccatga
    15841 aggagaaatt gtcggtatcg ttgatccgaa agaaacatct gaaaatgaat taggtctatt
    15901 gatggcgggc tataccttag aagaagcacg aaaagagtta gaaaaggggg ctggcgcaca
    15961 tgaatgatcg cagcgaaaaa ttccgtaatt tattagtgcc tgtactttca gttgttttag
    16021 gattgatctt aggcgcgata ttgatggctg cttttggttt tcagcccatc caaggttatt
    16081 cttctatgct agatgcagcc cttggttcgc aaagagggat aggtgaaacg ttaaggcaag
    16141 caacgccgtt gatttttaca gcattaggtt tttctgtagc taactcagct gggtttttca
    16201 atattgggct ttctggacaa gcactttgcg ggtggatcgt cagtgtctgg gtggcacttg
    16261 cgtttccaga tctgccaaaa gttgttttgc ttccgttatg cgtgatctta ggtgccttag
    16321 ctggtgcgat ggcagcggct gttccaggtt tacttcgagc attttttgga acaagtgaag
    16381 tgatcgtaac aatcatgatg aactatatct tattatacgt aagcacattt gctttacatg
    16441 aagtgatgcc tgaaagctat cgctcaagtt tggactcttc caatatgatc agtcaaaatg
    16501 cctcactgcg gttaccatgg ttaacacaag cttttggcgg ctctcgcgta aactcaggat
    16561 tatttttagc aatcatcgcg cttattttcg tttggctgct gatgaagaaa acgactttag
    16621 gatttgaaat ccgttctgtt ggattgaatc cttttgcttc tgagtatgca ggaatgagta
    16681 gtaaacgtac gatcgttttg tcgatggtca tttctggcgc tttggcaggg cttggcggtg
    16741 tggttgaagg attaggaacg tatcaaaact tctttgttca aacgacttcg ctatctattg
    16801 gatttgacgg aatggctgtt tccttgcttg gttctggatc agcaatcggt attcttttat
    16861 cagcgatctt gttcagtatt ttgaagatcg gtggacttgg catgcagaca ggagcaggtg
    16921 ttccgtttga gatcgtcaat gtatcgattg ctttgattat cttctttgtc gggatcaact
    16981 tcctgattcg tttcgtcatg gctaaagtct tccaagggaa aaaacaagaa gagatcgttg
    17041 cgacaataga atcgaaacca acgaatcaaa accatgaagg gggcgaacta taatgtcgac
    17101 tatcgaatta attgccttga tccttacttc tacactggtt tattcaacac cgctaattct
    17161 aacgtcacta ggcggaactt tttctgaacg aagcgggatc gtaaacgtag ggctagaggg
    17221 aatcatggtc atgggtgctt ttagttccat cgttttcaac ttgacgatga gtccatcact
    17281 tggttctatg acaccatgga tcgctatttt agtgggtggc gtcgtaggta tcctattttc
    17341 tctgattcac gcagtagcaa cagtcagttt gcgtgctgac catattatca gcgggacagt
    17401 aattaatctg atggcacctg ctttaggtgt tttcttgatc aaagcatggt atggtaaagg
    17461 tcaaactgat aacatcacgc agaatctggg gtatttttct ttcccaggat tagcagatat
    17521 tccggtcatt ggaccaattt tctttaaaaa tacattctta cctgcttatt gtgctatttt
    17581 gattgctgtc ctagcttggg tgatcatctt caagacgaaa ttcggcttgc gcctccgttc
    17641 agttggtgaa aatcctcaag ctgccgacac attaggtatc aatgtatatg ctatgcgtta
    17701 ttcaggcgta ctgatttctg gcttgttagg cggtatgggc ggtgctgttt ttgctcagtc
    17761 gatttccggt aacttttcag ctggtacgat cgttggacaa gggttcatct ccatggctgc
    17821 tatgatcttt ggtaagtgga atcctttagg ggcaatgggt gcctcattgt tctttggttt
    17881 tgctcaaagc ttgagtatca ttggcgcaca attaccagtt atttcttcga ttccgccagt
    17941 attgctgcaa attgctccat atttgctgac aatcattgtg ctggttgttt tcttagggaa
    18001 agcttctggt ccaaaagcaa acgggaagaa ctatatcaaa tcaaaataat cttttagcga
    18061 ataaaaaggg tgggttacgg gaagtgtttt tcgtctcgcc ctttttgttt taaaggcaat
    18121 aaaaataaaa agaaagaagg aataatcatg tttaaacgcg tacatctgat cgtcatggat
    18181 tcagtaggaa ttggagaagc accagatgcc gataagttcg gtgatgttgg ttctgataca
    18241 ttaggacaca ttgcgaaaga agcaggattg aacgttccac atttagagca gcttggttta
    18301 ggaacgatcc gtcctcttga aggtgtggaa tcagtgaaag atcatcaagg ctatgcgacg
    18361 aaattagaag aagtttctgt agggaaagat acgatgacag gccactggga aatcatggga
    18421 ttgaatatca aaactccatt tcgtgtgttt cctaatggat tccctgatga tctgatcaaa
    18481 caaatcgaag aattttctgg ccgtaaagtc gtatgtaaca aaccttatag cggaacagct
    18541 gttattgacg attacggtga acaccaaatg aaaacaggcg atttaattgt atatacttct
    18601 gccgatcctg tccttcagat cgcggcacat gaagaaatca ttcctttaga agaattatat
    18661 cggatttgcc aattcgttcg tgatataaca aaagatgatc cgtatatgat tggccgaatc
    18721 attgcacgtc cgtacgtggg tgaaccagga gacttcacac gaacaagcaa tcgccatgat
    18781 tatgcgttga atccattcgg acacacagtc cttgattctt tgaaagaagc aggaaaggac
    18841 gtgattgctg ttggaaagat caacgatatt ttcaacggac aaggaatcac tgaatctgtc
    18901 cgcacgaaaa gcaatatgga tggcgttgac cagctgctga aagttatgaa aaacgatttt
    18961 tcaggaatca gctttaccaa tttagtagat tttgatgcat tatacggtca tcgtcgggat
    19021 gtagtcggct atgcgcatgc gattgaagaa tttgacttac gaattccaga attgcttgaa
    19081 gcgatggaag aagacgactt gctaatgatt acagccgatc acggaaacga tccgacgttt
    19141 actggtaccg atcatacgcg agaatatgta ccgttattgg tttacagtaa aaaaatgaaa
    19201 gaacaagggg cattgcctca aggatattat tcagatattt cagcaacgat tgctgagaac
    19261 tttagtgttt cttcgactga aaatggcgaa agcttcttga aattattaaa ataatctttt
    19321 taaataggag aaagcaaatg accaagttaa gcgagatgtt acaagaaacg acccagttta
    19381 tccttgataa aggagtaaaa gaaattgatt ttggtttgat tctaggttca ggattaggtg
    19441 aattagcaga tgaaatcgaa gatgctgtaa tgatttctta tgatgagatc ccgttctttc
    19501 ctgtttcgac agtagttggg catgcaggaa agctagttta tgggtctctg gcaggcaaga
    19561 aagtgttagc catgcaaggg agattccact actatgaagg tcattccatg caaacagtga
    19621 cctatccagt acgtgtgatg gccggactag gggcccattc agtcattgtg actaatgcat
    19681 gtggtggtgt caacgaatca tttgtaccag gagatttgat gctgattacc gatcatatca
    19741 attttactgg tgaaaatcct ttgatcggtc cgaatgaaga tgcaatgggc cctagatttc
    19801 cagatatgag tcaagcttat acgcctgact atcaagcaac agcaaaagca gcagcagaaa
    19861 aactaggttt ggatcttaaa gaaggcgtat atatgggcta ttctggacca acttatgaaa
    19921 cgcctgcgga gatccgaatg tctcggacaa taggagccga tgcagtaggg atgtcgactg
    19981 ttccagaagt catcgtagca gctcacagcg gattaaaagt gttaggaatc tcttgtatta
    20041 ccaacttagc agcaggtatg caagcgaatc tgagccatga agaagttgta gaaacaactc
    20101 agagagtcaa acaatctttt aaagcattgg ttaaagaaac attgacatta ttgtaatagg
    20161 aggaacaata aaatgagtgt gcatattgaa gcgaaagaag gcgagatcgc cgataagatt
    20221 ttacttccgg gagatccttt gcgagctaaa tatattgcgg agactttttt ggaagatcca
    20281 gtttgttaca atcaggtacg cggtatgtta ggctttaccg gcaaatataa aggagaacgt
    20341 atttctgttc aaggaacagg aatgggaatg ccatcagcca ccatctatgc ccatgaattg
    20401 atccagtcgt atggtgtaaa gaaattgatt cgagtaggta catgcggtgc attgtcaaaa
    20461 gacgtccatg ttcgtgattt agtactagca caaggtgcag cgaccagttc ttcgatgatc
    20521 gaaaaaaatt tccaagcctt tcattttcct ccaatcagtg atttcaatct attactgaaa
    20581 gcatatgaga tagctaaaga aaagggctat acggttcatg taggaaatgt tctttcggaa
    20641 gattctttct acaaagatga tttgacagag acgttccaat tagctgaact tggcgtttta
    20701 ggagtggaaa tggaagcagc ggctctttat tacttaggcg cgaaatacca tgtgcagaca
    20761 ttaagtttga tgaccgttag cgaccatttg atcaccggtg aagaaacaac agctgcagaa
    20821 cgtcaatcta cgttcaatga aatgatcgaa gtaggattgg aaacagcgat agcagaataa
    20881 gaaggaaatg aaaaagctgc cctgcattaa gtaataagaa catccaagca aaaatagctc
    20941 ctcatgtttt tgacttggct ggacttatta ttgcaaatgt aagcttttga acaccgttta
    21001 ccaagaggtt gtgaatctgc cgtttaactc tgagtcacaa ggaacaattt caaaaa
//
LOCUS       DACECY010000228        21056 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.guided.049, whole genome shotgun sequence.
ACCESSION   DACECY010000228 DACECY010000000
VERSION     DACECY010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.guided.049"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IWO38_003021"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IWO38_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0315014.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2039..2227
                     /locus_tag="IWO38_003022"
     CDS             2039..2227
                     /locus_tag="IWO38_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0315015.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2285..2638)
                     /locus_tag="IWO38_003023"
     CDS             complement(2285..2638)
                     /locus_tag="IWO38_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0315016.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            3169..3591
                     /locus_tag="IWO38_003024"
     CDS             3169..3591
                     /locus_tag="IWO38_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0315017.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            3588..3818
                     /locus_tag="IWO38_003025"
     CDS             3588..3818
                     /locus_tag="IWO38_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0315018.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            4316..4516
                     /locus_tag="IWO38_003026"
     CDS             4316..4516
                     /locus_tag="IWO38_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0315019.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            4543..5736
                     /locus_tag="IWO38_003027"
     CDS             4543..5736
                     /locus_tag="IWO38_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0315020.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(5804..7369)
                     /gene="guaA"
                     /locus_tag="IWO38_003028"
     CDS             complement(5804..7369)
                     /gene="guaA"
                     /locus_tag="IWO38_003028"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ0315021.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(7428..8351)
                     /locus_tag="IWO38_003029"
     CDS             complement(7428..8351)
                     /locus_tag="IWO38_003029"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ0315022.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            8547..9152
                     /locus_tag="IWO38_003030"
     CDS             8547..9152
                     /locus_tag="IWO38_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0315023.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            9460..10476
                     /locus_tag="IWO38_003031"
     CDS             9460..10476
                     /locus_tag="IWO38_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0315024.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            10617..11918
                     /locus_tag="IWO38_003032"
     CDS             10617..11918
                     /locus_tag="IWO38_003032"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ0315025.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            11964..12626
                     /gene="deoC"
                     /locus_tag="IWO38_003033"
     CDS             11964..12626
                     /gene="deoC"
                     /locus_tag="IWO38_003033"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ0315026.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            12647..13045
                     /locus_tag="IWO38_003034"
     CDS             12647..13045
                     /locus_tag="IWO38_003034"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ0315027.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            13111..14193
                     /locus_tag="IWO38_003035"
     CDS             13111..14193
                     /locus_tag="IWO38_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0315028.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            14405..15967
                     /locus_tag="IWO38_003036"
     CDS             14405..15967
                     /locus_tag="IWO38_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0315029.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            15960..17093
                     /locus_tag="IWO38_003037"
     CDS             15960..17093
                     /locus_tag="IWO38_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0315030.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            17093..18049
                     /locus_tag="IWO38_003038"
     CDS             17093..18049
                     /locus_tag="IWO38_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0315031.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            18148..19314
                     /gene="deoB"
                     /locus_tag="IWO38_003039"
     CDS             18148..19314
                     /gene="deoB"
                     /locus_tag="IWO38_003039"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ0315032.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            19338..20156
                     /locus_tag="IWO38_003040"
     CDS             19338..20156
                     /locus_tag="IWO38_003040"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0315033.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            20173..20880
                     /gene="deoD"
                     /locus_tag="IWO38_003041"
     CDS             20173..20880
                     /gene="deoD"
                     /locus_tag="IWO38_003041"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0315034.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         6777 a         3760 c         4524 g         5995 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2221 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2281 ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg
     2341 atagttcttt gtctgtaaag ctatccatgt atttttctac ctgtaacctt cgagtgcttt
     2401 taatcaaatt atctgtaggt aagaaaaatt catcaataga tacatggagt aatgatacaa
     2461 gatcatacag cacttgtgta cttgggtgtt gtcctttgtt ttctatattt gttaaataac
     2521 gtgggtcaat ttcaatcatt gttcccactt gttcacgagt gagaccccgt ttcattcgag
     2581 cctctttaat cgctaaacca acggctctaa aatcgtattt atcttctttt ttacgcatac
     2641 taaatcacct ctatacattt tactgttcct atataaccat aaacaggtat agaaaaacat
     2701 gttatatagt ttatgagttc ctatagaaca ctgtggaatt tattaatgcg atgaatccaa
     2761 tttcaaatag aaaggtcgct gattttattt acctatacaa ctttataaaa tttagtcata
     2821 ctcctagaaa gtagataaaa ttggcgactt aatggaggga aaagtcgcca tgagaaagat
     2881 aatctgtcat ctagttctct taattaagtt cttatcaaaa aaacttatcc acctgtactg
     2941 gatagatatg accatcaatg tggttttgca gattacatag atagaaaaat caaagcatat
     3001 gaacagagaa aacaatctgt ttatgtgctt ttttggtcac ttaataaata tcttttagga
     3061 tttctcttca tttatgcaac aaatcccccg ttcgtgtcgg ggtttagtat gaagggagat
     3121 aaagaagcac atgccactcc ctttcacgac agaaaggggg tgaaaagcat gaaaccatct
     3181 tcttttcaag aaaccataga aaatcagttt gactatatct gcaaacaagt tattgaagat
     3241 gaacgaaaag attacttcaa acatctagac agactatcag caaaagaagt ctcgttttct
     3301 gatatagata actaccttgt caactctttt tctactgttg atacgtacgt cactgatttc
     3361 caactattta aattatttgg attaagaatc aatgttgaaa gtgatttatt aagtgaagcc
     3421 ctgcgtcatc tttcagagaa aaaacggaac attattttac ttcattactt tatggatatg
     3481 agtgatgtgg agattgcgga gttactaaat ttgaatcgtt cgactgttta tcgtcatcga
     3541 ataagtggac tagccatgat taaagaattc atgaaggagt gtaaagaatg aaaacagctt
     3601 atccaagggt tcccttcccc ctcattgtaa aggcgacaga tggcgacgtg gaagcaatta
     3661 atcagattgt taaacattat cgagggtata cctcaaaacg ttcattacgt cgcatgacag
     3721 acgaatatgg aaattctcat atggttattg atgaaaccct acgtggcaga atggaaacga
     3781 aactcatctc taagatttta tcgttcgaaa ttaaatagtc ccattttttc gtggaagcgt
     3841 gtcgaatcgt atccacgctt cccgatttgg gaggtttgtt attccatcaa agcatattgt
     3901 gtgttcagta ggttttgata ggctaacaag ccaaacgttc tttgacaacc aaataaaggc
     3961 aatcgaatac gtttcggtaa gaaaagagct gatgaactaa cacgccatga cctacaaaat
     4021 tagtgagcga ttcatgttag taattcgaaa gtaatcttta gcaggattgc tgccatgact
     4081 ttcttatcgt gataatgata ctcccgtata gccatagtcc gagcgttaga agcgtcacag
     4141 gcaatgggta cggctacatg agaaccatgc agaagtgaaa ttcctgtgag ctttgctaga
     4201 gctgttcgat tgctaagata ttcttctctt gtcagtgtat tattataaaa ctatttgata
     4261 taattatagg tgaatattgg agttttccta gcctataatt gggaaaggag taataatgat
     4321 aacggtcaca aaaaaggatt tagttgagct aggatatgga acttcttttt cagcagacat
     4381 cataaaaaaa gcaaaagaat tgatgattac aaaagggcat atctactatc aatctcgaaa
     4441 gctagataga gtccctcgtg aagctgttga agaaattcta ggaattcggt tttcaaatga
     4501 aaacaggtct gagtagtttt tgcacagtaa ggagtaaaaa ctatggttaa agacccaata
     4561 aaaaaagcaa agaatggaac ctattatttt cgtgcaaatc taggttatga ttctaaaggg
     4621 aaaaaaattc aaaaatatcg tagtggattc actactaaaa aagaagcaag agaagaatat
     4681 tcgaaactgc tattgatgaa accagaagaa ttaagtgaga ataaggataa aatgacgttt
     4741 aaacattata tctttgaaat tttccttcca tggtataaga cacaggtaaa gctcagaaca
     4801 tatgaaaatc gcttaccaac tattaagaag catttctctt attttgataa aatggctgtt
     4861 tccgatattg agcctattga tgtgcaaaat tggcaactaa aattatcaaa gaagtgtaag
     4921 tcttcttatg taagagctgt tcaaggacta ttttcagttg ctatggatcg agcaattgtc
     4981 cttgggataa ccaccacaaa tccatctaag attattggca atgtgaaaaa gcaaaagtct
     5041 aaaattgaat tttggacaaa agaagaattt gaaaaagtaa tctctctaat ttacaaagaa
     5101 gattactatc aacacttttt atttatctct ctgtggtttt tatttatgac tggaatgcga
     5161 attggtgaag ctactgccat tcaatgggaa gatattgatt ttgactcagg agtactaacc
     5221 atcaacaaaa cactctatta caagaatcaa tcgaattata gatttgttga accaaaaaca
     5281 aaggcgagcg ttcgtcatat tgtattagat aaatgcacat taacttattt atccgaatgg
     5341 aaggaagtac aacaaaatct aattcagaca gactttgtaa tgagctataa cggtattcct
     5401 acccaaaaac atacactatc atatgctatt gaacgctatg ctaagctggc tggtatccat
     5461 cgaatcagaa tccatgggtt acggcattct catgcttcct tgttaatcag tatgggagaa
     5521 aatccactta tcattaaaga tcgtctagga cacgaagata ttgagacgac tttaggaacg
     5581 tatggacatc tttatcctaa cagtaatttc gaggtagcta acaaattaaa tggagccatt
     5641 tcctttaaag aagcaaaaca aaattatgat acttcaccaa agaatcaatt tactgtagac
     5701 tacttgcgaa acaaagaaat gaaaaaagtg caataaaagt gcagtaaaaa gataagaaga
     5761 gccagaaacc cttggtataa gaggatttct agctctccta caactattcc cactccactg
     5821 ttgctggtgg tttagaagtg atgtcataca cgatacggtt gacgtgggct acttcgttta
     5881 cgatacgtac agagattttt tgcaagactt cccatgggat acgggcaaag tcagcggtca
     5941 ttccgtcgat tgaagttaca gcacggatac cgactgtgta gtcgtaagta cgtccgtcac
     6001 ccataacacc gacagagcgg atccctggaa gaacagtgaa atattgccag atatcacggt
     6061 caagtccagc agctgcgatt tcttcacgca agatagcatc agattcgcgt acgatttcta
     6121 gcttgtcttc agtgatttcg ccaagaacac gaataccaag accaggacca gggaatggct
     6181 gacgccaaac gattgaatca ggcatgccta actgagtccc taattcacgg acttcgtctt
     6241 tgaataatgt gtttaatggt tcgatcaatt caaattgcat atcttctggc agtccaccta
     6301 cattatggtg agatttgatc gtctgtgctg tttctgtacc agattcgata acgtcagtat
     6361 acaacgttcc ttgtgctagg aaagagacgc cttcttcacc agcaagtttt gttgcttcgt
     6421 catcaaaaac atatacgaat tcattcccaa tgattttacg tttttgttct gggtcagata
     6481 cacctttcaa cttgtctaag aagcgatctc ttgcatcgac tttgatgatg ttcaagccga
     6541 attttccgcc tagactttcc attacttgtt ccgcttcgcc tttgcgtagc agaccgtggt
     6601 caacaaagat agatgtcaat tggtcaccga tcgctttttg taaaagaacc ccaacgacac
     6661 tagaatcaac gccacctgaa agaccaagca agactttttt gtcaccaact tgttcacgga
     6721 ttttttctac ttgcatatcg ataaagttat ccattgtcca gttaccttca cagtgacaaa
     6781 catcaaaggc aaagtggcgc agcaattcga ttccgtattc ggaatgacgt acttctgcat
     6841 ggaattggat accataaaaa ttacgtgttg tgtcttggat agaagcaatc ggacaatctt
     6901 tgcttgttcc gactgtttca aagccttcag gaactttagt taccaagtca ccatggctca
     6961 tccatactgt ttgttgtttt ggtgtttctt taaacaagac agcttgatcg cttgttactt
     7021 ctaacaaggc tttcccatat tcgcggtttt tagcaggctc tacggaaccg ccaagtttgt
     7081 acgtgatcaa ttgcatacca tagcaaatcc ctaatacagg aatacctaaa ttgaagatct
     7141 caggatcgat cccaaatgca ttctcatcat atacactgtt tggtccgcca gaaagaatga
     7201 ttccctttgg attcatcgct tttacttctt ctgcagtaat gcggtggctc aataattcgg
     7261 aaaagactcc aagatcacga atacgtcttg tgatcaattg gttatactga ctaccgtagt
     7321 ctaatacgat aatcttttca acgttcgtca tatcggcaac gtttgtcaaa tttattaccc
     7381 ctatcccttt aaattttatc tcaaaatagt caaactattt taaaatgcta atattttcgc
     7441 atgaaaatcg aatcgatgat atgattttca gttttatgga gaattatatc ggctcttcct
     7501 cgtgtaggca agatatattc ctgtaaattt ttcaaattca catttttcca tacatctttt
     7561 gccattttaa aggcttcttc acgatctccg atagcaaaac gataataata attctctgga
     7621 ttttggaaag ccgtatccag taacgcacca aaacgatcca aataccattt ttcgatcagc
     7681 acgggatcgg catcgacata gacagaaaaa tcaaaaaagt cgctgacgta gatttgctga
     7741 ttcgcaggta attgaagcgt attgatcccc tcaacgatca agatatcagg ctgctggatc
     7801 aattcgtact tgtctggaat gatatcatac acatcatgtg agtaaacagg tacttttatc
     7861 tcttcctgac cagattttac acggttcaag aaatcgatca gcatctccat atcataactt
     7921 tcaggaaatc ctttacggtc catgattcca cgctctttta atatacgatt tggataaaga
     7981 aatccgtcag tcgtgatcag ctgaacgttt cttcgtttga aggtacgaga caaaatcgtc
     8041 tgcagcagtc tggctgttgt actttttcca acggctacac ttccggcgat cccgataata
     8101 aaaggcggta cttctacgta tttatgaaga aataaacctt tactcaagaa caaagattca
     8161 aattctttca tataaagata aatcagatga gcaagaggta catagatatc tcgaacatcc
     8221 tgcatggaaa tgcggtcatt caaacttttg atattatcta gttcatcttg tgtcaaaggt
     8281 gcttgcccat tacgataaaa ttctcgccat tcttctcttg ccacacggta gtagttcatt
     8341 ggatcattca tattagcctc ctgcgttctc ccatcaatgg aactatttta acacaaaata
     8401 aacgcaaacc ccaccactta agagagattg ccgatctttt tgtataatct ttataataga
     8461 aagaaacgga gtcttattct tgacttgcgc acggatacat aaaaggatag actgttgtca
     8521 tgaaagaaat gagaaggatg gaagacatga caaatcatta ctattctgaa cgcccagata
     8581 cggcgcacga ttttgaacaa tgggcttttg agttaaaagg cagaaccttt caatttgtaa
     8641 cagattctgg tgtattttca cgtgaaacag tggattatgg ttcacgtgtc ctgattgatg
     8701 ctttcgattg ggaagagtta cctgatgaag gaaagatctt agatgtggga tgcgggtatg
     8761 gaccgattgg gttagctttg gcatttgcga cacagcggtt tgttgaaatg gtcgacatta
     8821 atgaacgagc ggtaagtcta gcgcaaggaa acgcaaagcg taaccaaatc gaacatgttg
     8881 atatccatca atcgaatatc tatgaagcag ttcatgaaga aacctatgca gctatcgtaa
     8941 gcaatccgcc gatccgtgct ggaaaaaaag ttgtacatga aattttgaca ggcgcatatc
     9001 ctcggttgaa gaagggcggt acactgacaa ttgtgatcca gaagaaacaa ggagcgccga
     9061 gtgcgcaaaa gaaaatggaa gaaacatttg gtaatgcaga aatcgtcaca aaagataaag
     9121 gatattatat catcaaaagt gtgaaagaat aactagctga aaaatagtgg agcgacgaaa
     9181 gagtggaact gggcgtgcat gtacgggtac gtatcagttc tttttttgtt gagaagtttt
     9241 taaaaacaaa gaactatttt ttcttcaaaa agaaatagta ggaacagagt cgttgttttt
     9301 tacgataaaa taaataagat acagaacttg tatagaaggc tgccaagtcg gtagataaag
     9361 gaggttttta tcaaaaaaca tactttcctt ttttctctat tcttttcttg ggggttttgg
     9421 tacactgata gggaatttag atagaaggga gccaatagga tgaagctgac catcagagat
     9481 atcgcagaaa tggcaggcgt atcagtaaca actgtctcac agattttgaa taacaaagga
     9541 agcagattta gtgataagac aagaaaaaaa gtgttgtcga tcgtgaatga acatcattat
     9601 aagcccgatt atttcgcttc aaatgtgatc aatcgacatt caaagaccat cggaatgatc
     9661 gtccctgatg tcactgattt cttcttctcc aaactgatcg aaggtgtaga gagctatttg
     9721 aatcctttgg gctatgtgat catgttatgc aattctcgtc atagccaaga aaatgagatc
     9781 aagtatttac aagagctttc ccaccgctca gtggacggaa tcttgctggc cacaccaaat
     9841 attttacctg agaaatatgc attagacagc ggtttttatc aaaaaatgcc aatcatttta
     9901 atcgatcgcg gattgaatcg tcgggataac ggacggctta tcgtaaagga atatgagggc
     9961 gcctatcaag ctgtttcgct gtttattgaa aatggccata cgaagatcgg catgctgaag
    10021 gaaaccactg gatactatca attagaagaa cgtttcaacg gatatcgtca tgcgctgaaa
    10081 gatcatggcc tgccttttaa tgggaaattt gttgaacatg gagagttgac tgtccaaggt
    10141 ggatatactg catcaaaaaa attactcaaa cataaagata tcacggctat tttttgcggg
    10201 aatgatgcca tggcgatcgg atgctatcag gcgatcgatg aattgggcaa aaaaatccca
    10261 gaagatatct cagtcatagg attcgatgga ttaaagctgt cagaatacat gattccgcaa
    10321 ttgacgacag tgcagcagcc aagttttgat attggatttt atgctgcacg tttcctgatc
    10381 gatacgatcg aatttcctca aagaaaagtt ccgaacaaag tgttcgaaac aaaattaatt
    10441 attcgtgaga gcgtaaaagt attgacaaag ggctaactcg gcggtatatt gcttatgtaa
    10501 gcatttacaa aggctagtta gtgctttttt tatttttagt aaaccagttt actaaagtgg
    10561 tttacctttc ctaaaaataa tgtataatac cgaatgaaga caaggaggat caggcaatgc
    10621 gaatggtgga tctgattgaa aaaaagaggg atggaaacga gctgtcgaaa gaggaaatcg
    10681 agtacatcgt cacgaactat acgaatggaa agattccaga ttatcaagtc agtgcattat
    10741 tgatggcaat tttttatcaa gacatgacaa atgaagaaat cacgaactta acattggcga
    10801 ttgctaattc tggtgatgtc atcgatttga gttctcttga aggaataaag gtagataagc
    10861 attcgacagg tggcgttggt gacacgacca cattgatttt agccccccta gtagctagtg
    10921 tcggagtgac agtggctaaa atgtctggac gcggtctagg ttacacaggt ggtacactag
    10981 acaagctaga agcaattccc ggatttcaga tcgagctttc ggatgaagaa tttgtacgca
    11041 ttgttaatga aagtaaagta gcggtaattg gtcaatcagg aaacttggca ccggcggaca
    11101 aaaaattgta tgccttgcgt gacgtcacag cgaccgttga ttctcttccg ctgattgcca
    11161 gttcgattat gagtaaaaaa atcgcggcag gagcagatgc aatcgttttg gatgtgacca
    11221 ctggagacgg tgctttcatg aagaacatcg aagatgcaag acgtctggca aagaccatga
    11281 cgagcattgg gaaactggct aaccgagaaa cagtggcggt tatctcagat atgtcagaac
    11341 ctcttggcga agctatcgga aatagtctag aagttgtcga ggcaatcgaa acattacaag
    11401 gaaatggtcc agaagatcta gtagaaatgt gctacgcctt aggaagccaa atggttgttc
    11461 ttgctggcaa agctaagaca accgatgaag caagaacgtt gcttcaagaa gctctggaat
    11521 caggtaaagc gttagcaaaa ttcaaagaaa tgatccaaaa tcaaggggga gacccagcga
    11581 tcgtggaaca tccagaacga attttgactg cacgctacac aatggagctt cctgctaaac
    11641 aaagcggagt tgtgtcaaaa atcgtggcaa atgaattagg aattgctgca atgatgctag
    11701 gtgctgggcg taaaaccaaa gaagaagata ttgatcacgc tgttggattg aaactgcata
    11761 aaaaaattgg tgacactgtg acaaaaggtg agtcactatt gacgatctat agtaacgacg
    11821 aagaaatcac ttctgtcatt gaattattat ataaaaatat tgaaattggt gaatcggcaa
    11881 tgaagccaac gttgattcat gacatcatca cagaataaag gaaaaaagcg gactttaccg
    11941 ttttaaagaa aaggggaaaa gaaatggaac taaaccgaat gatcgaccat actattttga
    12001 aagcagacgc atctaaagaa gctgtgatgc aaattatcga agaagcaaaa aaataccatt
    12061 tttattctgt ctgcatcaat ccagcatggg ttgcgctagc gaaagaacaa ttacaaggtg
    12121 agccagtagc cgtatgtaca gtaatcgggt ttcctctagg agcaaacaca cccgagacaa
    12181 aagcatttga aacaacagat gccatcaata acggtgcaga tgaagtcgat atggtcatca
    12241 atatcggtgc tttgaaatca aaggatgatc aacaagtcca aaaagatatc gaagcagttg
    12301 ttgaagcagc aaaagatcgt gcgcttgtca aagttattat tgaaacatcg ttattagata
    12361 gagaagaaat catccgagct tgcgaaatag caaaagctgc aggcgctgat tttgtcaaaa
    12421 catcaactgg tttctcaaca ggcggagcaa aggtagagga tgtccgtttg atgcgtgaaa
    12481 ctgtaggacc agaaatgggt gtcaaagctt ccggcggtat ccataacgaa gaagaagcaa
    12541 tggcaatgat cgaagctggt gctacaagaa tcggaacaag cgcaggagta gcaatcgtat
    12601 ctggttcaac tggtgaaggc tattaatttt ttcaaaggag aagctcatgg cagaactcaa
    12661 acaagaatgg atcaacacag ctattgaagc gttagataaa gcctatgttc cttattccca
    12721 ttttccggta ggcgcttgtc tcgtaacgga atcaggcaag atttatcaag gcatcaacat
    12781 tgagaatgca tcatttggat taacgaattg tgccgagcgc acagcttttt tcaaagcagt
    12841 atctgaagga gaaaaaacat ttacgcatct agtcgtagcg gggcacaccc ccgatcctat
    12901 ctctccttgt ggtgcatgtc gacaagtaat ggcagaattc tgttcccctg atatgccggt
    12961 tacgctagta ggagacaatg gtgtgacgaa ggcaacgacc gttcgtgcac tgctgcctta
    13021 tgcatttact gaaaaagatc tataactcat gttcattcag tcagctgact gttgaaaaat
    13081 aaaacaatta ttttaggagg ctttacagaa atgaaaaaag caaaattatt tggtttaggt
    13141 gctgtcgcac tagcagcagg gttattttta ggtgcatgcg gcaacaacgg ctctactgat
    13201 tcatccggtg gcaaaagctc atcagacaca acaactgcag cattgatcac agacacaggt
    13261 ggtgtagacg accgttcatt caaccagtca gcttgggaag gactagaaaa atgggggaaa
    13321 gatcaaggac tttcacgagg aaatgatggc ttccaatatt tccaatcttc aaatgaatca
    13381 gattacatcc caaatattga ccaagcattg aatgctggat tcaaaacgat ttttggtatt
    13441 ggttataaac taaaaccagc gatccaagaa caagcaacaa acaatacagg tacgaacttt
    13501 gttattatcg atgatgtcat cgatggatta gataacgttg tatctgcaac tttcaaagac
    13561 aacgaagctt cttatcttgc aggtgttgca gcagcttata cgactgaaac aaacgtagtc
    13621 ggctttatcg gcggtgtaaa aggtgaagta atcgatcgtt ttgatgctgg attcaaagcc
    13681 ggtgttgatg caggtgcgaa agaattagga aaagaaatca aagtattgaa tcaatatgct
    13741 ggtgacttct ctgctccaga taaaggacgt tcaattgctc aaggtatgta tgcacaaaac
    13801 gcagatatca tcttccatgc ttcaggcggt acaggtaacg gtgtattcca agaagcaaaa
    13861 tcattgaacg aatcaggcga caaaaaagtt tgggttatcg gtgttgaccg cgaccaatca
    13921 gacgaaggcg aatacacatt aaacggcgaa aagaaaaact tcacattgac atctacactg
    13981 aaagcagttg gaacagtagt agaagatttg gcacaaaaat cagcggacgg taaattccct
    14041 ggcggcgaac acactgttta tggattgaaa gaagacggtg ttggtctaac agaaggacaa
    14101 ctttctgacg aagcgaaaaa agcagttgat gaagcaaaag aaaaaatcat ttcaggtgac
    14161 gtaaaagttc cagaaactcc tgaagaaaac taaaaccatt tctatagcct tgttagacac
    14221 atgtttgaca aggctatggt ttgattagaa caagaattgt tcatttatcc aagctgcctt
    14281 gtgacagttt ggataaggga acaactccct gaagtaatgt ttgtttcaaa agaagaacgc
    14341 tcactaagcg tgaatcttag tcaatcgttg ccctagatag aaatcgttag gatgtgaaaa
    14401 aaaggtggtt caagaaaata ttgtcataga aatgcagaat attaccaaag aatttggaac
    14461 attcaaggca aatgacaaca tcaatttaca agtaaaggca ggagaaattc atgcgttgct
    14521 tggagaaaac ggtgctggca aatctacatt gatgaacgtg ctttccggat tattagagcc
    14581 gacatcaggg aaaattttga tgcgtgggaa agaagtaaag atcacaagtc cgaccaaagc
    14641 caaccaatta gggattggga tggtccatca gcactttatg cttgttgatg cctttactgt
    14701 aacagaaaac atcgtgttgg gaagcgagcc tagtcgcgca gggatgcttg accataaaaa
    14761 agcacgaaaa gagatccaaa aagtttctga acagtatgga ttgtcagtca acccagatgc
    14821 ttacgttcgt gatatttcag ttggaatgga acaacgggta gaaattttaa aaacacttta
    14881 ccgaggagca gatgtactga tttttgatga gccgacagct gtattgaccc ctcaagaaat
    14941 tgatgaatta atcgtgatca tgaaggaatt agtcaaagaa ggcaagtcga tcattttgat
    15001 tacgcataag ttagatgaaa tcaaagcagt agctgaccgc tgtacagtta tccgccgtgg
    15061 aaaaggaatc ggtacagtca acgttaaaga cgtaacctca caacaattag ccgatatgat
    15121 ggttggaaga acggtttcat tcaaaacgat gaaaaaagaa gcgaagcctc aagaagtcgt
    15181 tttgtctatt gaaaatctag tggtgaaaga aaatcgtgga ttagaagccg tgaaaaactt
    15241 gaacttagag gttcgtgctg gcgaagtact tggtatcgct ggaatcgatg gaaacgggca
    15301 gtcggagttg atccaagctt tgactggttt gcgaaaggca gaaagcggac atatcaagct
    15361 aaaaggggaa gacatcacta ataaaaaacc ccgaaaaatc actgaacatg gtgtaggaca
    15421 cgtgccagaa gaccgtcata aatacgggtt agtcctagat atgacattat ctgaaaatat
    15481 cgcactacaa acctatcatc aaaaacctta cagtaaaaac ggcatgctga attattcagt
    15541 aatgaatgaa catgccagag aattgatcga agaatatgat gttcgaacaa cgaatgaact
    15601 tgttcccgca aaagctttat caggcggaaa tcagcaaaaa gcaattatcg ctcgtgaagt
    15661 cgaccgagat cctgatctgt tgattgttgc aaatccaact cgtggactgg atgtaggtgc
    15721 gatcgaattt attcataaac gtctgatcga acaaagggac aaatacaaag cagtgttact
    15781 gatcagtttc gaattagaag aaattttaaa tgtttcggat cgtattgctg ttatccatga
    15841 aggagaaatt gtcggtatcg ttgatccgaa agaaacatct gaaaatgaat taggtctatt
    15901 gatggcgggc tataccttag aagaagcacg aaaagagtta gaaaaggggg ctggcgcaca
    15961 tgaatgatcg cagcgaaaaa ttccgtaatt tattagtgcc tgtactttca gttgttttag
    16021 gattgatctt aggcgcgata ttgatggctg cttttggttt tcagcccatc caaggttatt
    16081 cttctatgct agatgcagcc cttggttcgc aaagagggat aggtgaaacg ttaaggcaag
    16141 caacgccgtt gatttttaca gcattaggtt tttctgtagc taactcagct gggtttttca
    16201 atattgggct ttctggacaa gcactttgcg ggtggatcgt cagtgtctgg gtggcacttg
    16261 cgtttccaga tctgccaaaa gttgttttgc ttccgttatg cgtgatctta ggtgccttag
    16321 ctggtgcgat ggcagcggct gttccaggtt tacttcgagc attttttgga acaagtgaag
    16381 tgatcgtaac aatcatgatg aactatatct tattatacgt aagcacattt gctttacatg
    16441 aagtgatgcc tgaaagctat cgctcaagtt tggactcttc caatatgatc agtcaaaatg
    16501 cctcactgcg gttaccatgg ttaacacaag cttttggcgg ctctcgcgta aactcaggat
    16561 tatttttagc aatcatcgcg cttattttcg tttggctgct gatgaagaaa acgactttag
    16621 gatttgaaat ccgttctgtt ggattgaatc cttttgcttc tgagtatgca ggaatgagta
    16681 gtaaacgtac gatcgttttg tcgatggtca tttctggcgc tttggcaggg cttggcggtg
    16741 tggttgaagg attaggaacg tatcaaaact tctttgttca aacgacttcg ctatctattg
    16801 gatttgacgg aatggctgtt tccttgcttg gttctggatc agcaatcggt attcttttat
    16861 cagcgatctt gttcagtatt ttgaagatcg gtggacttgg catgcagaca ggagcaggtg
    16921 ttccgtttga gatcgtcaat gtatcgattg ctttgattat cttctttgtc gggatcaact
    16981 tcctgattcg tttcgtcatg gctaaagtct tccaagggaa aaaacaagaa gagatcgttg
    17041 cgacaataga atcgaaacca acgaatcaaa accatgaagg gggcgaacta taatgtcgac
    17101 tatcgaatta attgccttga tccttacttc tacactggtt tattcaacac cgctaattct
    17161 aacgtcacta ggcggaactt tttctgaacg aagcgggatc gtaaacgtag ggctagaggg
    17221 aatcatggtc atgggtgctt ttagttccat cgttttcaac ttgacgatga gtccatcact
    17281 tggttctatg acaccatgga tcgctatttt agtgggtggc gtcgtaggta tcctattttc
    17341 tctgattcac gcagtagcaa cagtcagttt gcgtgctgac catattatca gcgggacagt
    17401 aattaatctg atggcacctg ctttaggtgt tttcttgatc aaagcatggt atggtaaagg
    17461 tcaaactgat aacatcacgc agaatctggg gtatttttct ttcccaggat tagcagatat
    17521 tccggtcatt ggaccaattt tctttaaaaa tacattctta cctgcttatt gtgctatttt
    17581 gattgctgtc ctagcttggg tgatcatctt caagacgaaa ttcggcttgc gcctccgttc
    17641 agttggtgaa aatcctcaag ctgccgacac attaggtatc aatgtatatg ctatgcgtta
    17701 ttcaggcgta ctgatttctg gcttgttagg cggtatgggc ggtgctgttt ttgctcagtc
    17761 gatttccggt aacttttcag ctggtacgat cgttggacaa gggttcatct ccatggctgc
    17821 tatgatcttt ggtaagtgga atcctttagg ggcaatgggt gcctcattgt tctttggttt
    17881 tgctcaaagc ttgagtatca ttggcgcaca attaccagtt atttcttcga ttccgccagt
    17941 attgctgcaa attgctccat atttgctgac aatcattgtg ctggttgttt tcttagggaa
    18001 agcttctggt ccaaaagcaa acgggaagaa ctatatcaaa tcaaaataat cttttagcga
    18061 ataaaaaggg tgggttacgg gaagtgtttt tcgtctcgcc ctttttgttt taaaggcaat
    18121 aaaaataaaa agaaagaagg aataatcatg tttaaacgcg tacatctgat cgtcatggat
    18181 tcagtaggaa ttggagaagc accagatgcc gataagttcg gtgatgttgg ttctgataca
    18241 ttaggacaca ttgcgaaaga agcaggattg aacgttccac atttagagca gcttggttta
    18301 ggaacgatcc gtcctcttga aggtgtggaa tcagtgaaag atcatcaagg ctatgcgacg
    18361 aaattagaag aagtttctgt agggaaagat acgatgacag gccactggga aatcatggga
    18421 ttgaatatca aaactccatt tcgtgtgttt cctaatggat tccctgatga tctgatcaaa
    18481 caaatcgaag aattttctgg ccgtaaagtc gtatgtaaca aaccttatag cggaacagct
    18541 gttattgacg attacggtga acaccaaatg aaaacaggcg atttaattgt atatacttct
    18601 gccgatcctg tccttcagat cgcggcacat gaagaaatca ttcctttaga agaattatat
    18661 cggatttgcc aattcgttcg tgatataaca aaagatgatc cgtatatgat tggccgaatc
    18721 attgcacgtc cgtacgtggg tgaaccagga gacttcacac gaacaagcaa tcgccatgat
    18781 tatgcgttga atccattcgg acacacagtc cttgattctt tgaaagaagc aggaaaggac
    18841 gtgattgctg ttggaaagat caacgatatt ttcaacggac aaggaatcac tgaatctgtc
    18901 cgcacgaaaa gcaatatgga tggcgttgac cagctgctga aagttatgaa aaacgatttt
    18961 tcaggaatca gctttaccaa tttagtagat tttgatgcat tatacggtca tcgtcgggat
    19021 gtagtcggct atgcgcatgc gattgaagaa tttgacttac gaattccaga attgcttgaa
    19081 gcgatggaag aagacgactt gctaatgatt acagccgatc acggaaacga tccgacgttt
    19141 actggtaccg atcatacgcg agaatatgta ccgttattgg tttacagtaa aaaaatgaaa
    19201 gaacaagggg cattgcctca aggatattat tcagatattt cagcaacgat tgctgagaac
    19261 tttagtgttt cttcgactga aaatggcgaa agcttcttga aattattaaa ataatctttt
    19321 taaataggag aaagcaaatg accaagttaa gcgagatgtt acaagaaacg acccagttta
    19381 tccttgataa aggagtaaaa gaaattgatt ttggtttgat tctaggttca ggattaggtg
    19441 aattagcaga tgaaatcgaa gatgctgtaa tgatttctta tgatgagatc ccgttctttc
    19501 ctgtttcgac agtagttggg catgcaggaa agctagttta tgggtctctg gcaggcaaga
    19561 aagtgttagc catgcaaggg agattccact actatgaagg tcattccatg caaacagtga
    19621 cctatccagt acgtgtgatg gccggactag gggcccattc agtcattgtg actaatgcat
    19681 gtggtggtgt caacgaatca tttgtaccag gagatttgat gctgattacc gatcatatca
    19741 attttactgg tgaaaatcct ttgatcggtc cgaatgaaga tgcaatgggc cctagatttc
    19801 cagatatgag tcaagcttat acgcctgact atcaagcaac agcaaaagca gcagcagaaa
    19861 aactaggttt ggatcttaaa gaaggcgtat atatgggcta ttctggacca acttatgaaa
    19921 cgcctgcgga gatccgaatg tctcggacaa taggagccga tgcagtaggg atgtcgactg
    19981 ttccagaagt catcgtagca gctcacagcg gattaaaagt gttaggaatc tcttgtatta
    20041 ccaacttagc agcaggtatg caagcgaatc tgagccatga agaagttgta gaaacaactc
    20101 agagagtcaa acaatctttt aaagcattgg ttaaagaaac attgacatta ttgtaatagg
    20161 aggaacaata aaatgagtgt gcatattgaa gcgaaagaag gcgagatcgc cgataagatt
    20221 ttacttccgg gagatccttt gcgagctaaa tatattgcgg agactttttt ggaagatcca
    20281 gtttgttaca atcaggtacg cggtatgtta ggctttaccg gcaaatataa aggagaacgt
    20341 atttctgttc aaggaacagg aatgggaatg ccatcagcca ccatctatgc ccatgaattg
    20401 atccagtcgt atggtgtaaa gaaattgatt cgagtaggta catgcggtgc attgtcaaaa
    20461 gacgtccatg ttcgtgattt agtactagca caaggtgcag cgaccagttc ttcgatgatc
    20521 gaaaaaaatt tccaagcctt tcattttcct ccaatcagtg atttcaatct attactgaaa
    20581 gcatatgaga tagctaaaga aaagggctat acggttcatg taggaaatgt tctttcggaa
    20641 gattctttct acaaagatga tttgacagag acgttccaat tagctgaact tggcgtttta
    20701 ggagtggaaa tggaagcagc ggctctttat tacttaggcg cgaaatacca tgtgcagaca
    20761 ttaagtttga tgaccgttag cgaccatttg atcaccggtg aagaaacaac agctgcagaa
    20821 cgtcaatcta cgttcaatga aatgatcgaa gtaggattgg aaacagcgat agcagaataa
    20881 gaaggaaatg aaaaagctgc cctgcattaa gtaataagaa catccaagca aaaatagctc
    20941 ctcatgtttt tgacttggct ggacttatta ttgcaaatgt aagcttttga acaccgttta
    21001 ccaagaggtt gtgaatctgc cgtttaactc tgagtcacaa ggaacaattt caaaaa
//
LOCUS       DACECY010000229        20985 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.guided.050, whole genome shotgun sequence.
ACCESSION   DACECY010000229 DACECY010000000
VERSION     DACECY010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.guided.050"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(134..577)
                     /locus_tag="IWO38_003042"
     CDS             complement(134..577)
                     /locus_tag="IWO38_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ0315035.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="IWO38_003043"
     CDS             complement(795..2135)
                     /locus_tag="IWO38_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0315036.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="IWO38_003044"
     CDS             2404..3300
                     /locus_tag="IWO38_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ0315037.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="IWO38_003045"
     CDS             complement(3450..4391)
                     /locus_tag="IWO38_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0315038.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="IWO38_003046"
     CDS             4517..5638
                     /locus_tag="IWO38_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0315039.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYSLGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /locus_tag="IWO38_003047"
     CDS             5896..7398
                     /locus_tag="IWO38_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ0315040.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="IWO38_003048"
     CDS             7465..7992
                     /locus_tag="IWO38_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0315041.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="IWO38_003049"
     CDS             8203..9264
                     /locus_tag="IWO38_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0315042.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="IWO38_003050"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="IWO38_003050"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ0315043.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="IWO38_003051"
     CDS             10177..12159
                     /locus_tag="IWO38_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0315044.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="IWO38_003052"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="IWO38_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ0315045.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="IWO38_003053"
     CDS             12881..14305
                     /locus_tag="IWO38_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0315046.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="IWO38_003054"
     CDS             complement(14486..15259)
                     /locus_tag="IWO38_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0315047.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="IWO38_003055"
     CDS             complement(15256..16161)
                     /locus_tag="IWO38_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0315048.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16315..16635)
                     /locus_tag="IWO38_003056"
     CDS             complement(16315..16635)
                     /locus_tag="IWO38_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ0315049.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17109..17795
                     /locus_tag="IWO38_003057"
     CDS             17109..17795
                     /locus_tag="IWO38_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ0315050.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            17797..18741
                     /locus_tag="IWO38_003058"
     CDS             17797..18741
                     /locus_tag="IWO38_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ0315051.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            18940..19584
                     /gene="pcp"
                     /locus_tag="IWO38_003059"
     CDS             18940..19584
                     /gene="pcp"
                     /locus_tag="IWO38_003059"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ0315052.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            19663..20940
                     /locus_tag="IWO38_003060"
     CDS             19663..20940
                     /locus_tag="IWO38_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315053.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         6779 a         3784 c         4360 g         6062 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttattcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcaccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    16261 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    16321 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
    16381 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
    16441 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
    16501 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
    16561 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
    16621 ttccatccat gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa
    16681 aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat
    16741 tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt
    16801 agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg
    16861 caatttcata gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct
    16921 tatttctata tattatcggg caataaatta aaaaaagctt taccttacaa taagtaagat
    16981 aatttttgtg ataaaaaaat aacgaaaaat atttaaaaat agtgtctatt tcgtgagaaa
    17041 acaactatat tttttatgtg ttttgcttta taattaaaaa aatattgtca ttttatggag
    17101 gaaaatatat ggaaaattat cttagccttt taggcgtctt gattgttatt ctcgggtttg
    17161 catttaaatt agactcgata ttgatcgtaa tggttgcttt agttgttaca gctttgactg
    17221 gcggtcttgg cattcaagga atgttagaac ttttaggtac aagttttgta aacaatcgcg
    17281 ggatggcgat ttttatcatc attatgttgg caacaggaac gcttgaaaga aatggtctga
    17341 aagaatcagc ggcttccttg atcaagcgat tcaagaaagt atctgctggt atgattgtag
    17401 atatttatgg gatctttcga atgatctttg cggcttttaa cgtgagtttt ggtggagttg
    17461 ctggatttgt tcgtccaatc attttaccga tggcattggg gaccatcgaa tcaaaagggt
    17521 tagaagtcca tcctaagcat gaagaagaac taaaaggtat ggcttcagct atggaaaatg
    17581 tctgctggtt ctttggccaa gtactattca tcggaggagc aggggcgttg ctagtccaat
    17641 caacactgaa agatctagga tatgacgtaa cattgggaca gcttgcttta gtggaagttc
    17701 cagtggctat cacggcttta gttgtggcaa gtgtctactt ctatcttaaa gaaaagaaat
    17761 tagcgaagaa atattatcca ggaaaggggc aataagatgg acttttttac aaatcctgat
    17821 attttattag gcgataaatt actggagatc ctttatatcg tgatgggact cgtattgatt
    17881 tacactggag taagaaattt aacagataaa accaatcctc atcgttatgg gtcagccttt
    17941 ttctggacgg tcttaggggt agttattgca ggaggaagat ggcttccggc aatcgtgaac
    18001 ggtggcttga tctttgcgat gaccatacca gcaattgcta agaaagtcag caaaggagaa
    18061 agccggcttc catcaaaaga atatatggaa aaaatgtcag acaaactagg aatgaaaatt
    18121 tttattccag ctttgagtat cggtgtcttt gcgatcttgt ttgctctatt taccaattta
    18181 ggtgctttag tcggcgtggg cgtcggtgtt tttgcagcca tgctaatctt gatgttcttt
    18241 tctcgagata atcgaccaag tacattttta gatgatgcag cagatatgct gggcacagta
    18301 gggccgttaa gcatgttgcc aatgctttta gcttctctag gtgcggtatt tacaagcgct
    18361 ggagttggga cagtgatttc caatgcagta ggtacgatcg ttccagaagg aaatgtgaac
    18421 atcggcatca tcatttacgc tcttggcatg gtgatattta cggtaatcat ggggaatgca
    18481 tttgcggcca tcaccgtgat gacggttggg atcggtgcac cattcgtatt tagtcatgga
    18541 gcgaatccag cgttaatagg aatggttgca ttgacttgcg gtttttgtgg gaccctgctg
    18601 acaccgatgg ctgctaactt caatatcgtg cctgtagcga tgctggagat gaaagacaaa
    18661 tacggagtga tcaaaaacca gttgtttatt gcgttattta tgctagtttt ccaaattgct
    18721 tatatgattc tatttaaata atagaagaga ggggttgtga cagaataatt gtcgcaacct
    18781 cttctaacat tgtagtggaa cactattttt ttataagaat atattaagtt tgatctgtta
    18841 tgttatttaa actattgttt cctttattta cgctgttcaa ggaaaatggt atgataaaag
    18901 ataaccatat gttaaatcaa tcggacggag gaacaacaga tgaaagtact agtaacaggt
    18961 tttgatccat ttggtgggga caaagtgaat ccagcctatg aagcagtaaa aaaaatgcca
    19021 gatgaaatca gcggtgctga aatcatcaaa gtggaagtac cgaccgtatt cgaaaaaagc
    19081 agtcaagtag tcaaagaagc catccaacag caccaaccag atgtcgtgat ctgtgtcgga
    19141 caagcaggtg ggcggtcagc agtctcgttc gaacgtgtcg caatcaatct agcagaagca
    19201 agaatccctg ataatgaagg aaatcagcca ttcgatacgg ctttagaaga aaacggtcca
    19261 gccgcttact ttacctcatt gccaatcaaa gcaatgacaa aaaatgtaca agatcatggt
    19321 ctaccagcgt acatctctta tacagcagga acatttgtct gcaatgatat catgtatcgt
    19381 ttgcttcatt tgatcgaaac acaattcccg gcaattcgag gtgggttcat ccacgtgcca
    19441 ttctcacccg atcaagtgat cgatcgtcca gtgggtacgc cttctatgtc tttagaagac
    19501 attgcttctt ctttgactta tgcagttgaa gcagcagtag aaaataaaga agatatttcc
    19561 ggaaatgccg gaacaactca ctaatttagc aagaaacact ggctggttgc taacgttatg
    19621 ttagtagcca gctttttgta attagaaagg aaagaaacag ccgtggaaga atggcaatcc
    19681 gtctttgaag aatggtttcc aaaagaaatc agcaaaagct atcccatcaa aatttctaaa
    19741 caatacacaa gcagtcaacg ttgggagatc tatgcaaaat taacgaaaaa acagcgggaa
    19801 ctagtcgata agcaccgccg atatctaatc agctctcgtt ttatggaaga acattattta
    19861 gctgcaacag attgggtgtt ttctgacttc aagatcaatc cgttttttcg aacaaaacga
    19921 agccagcaaa aattgtattg tgagtgtggt agagaattaa aagtgcaata catcgttaaa
    19981 tcgccaaaaa ccggaaagat cctaaaacta ggcatcaacc actttgccga tcacctgcat
    20041 gtctctccga cagtggcagc ttcgatccat cagggaatga caaaagttga tctggcctta
    20101 gacgaattat tatggcttaa gcagaagaat atcgattttc ctgaaggact atggcaaaaa
    20161 tactgttttg tcttgtacca aaatcgtcgg atgaaacagc catatttacc ggatataaaa
    20221 ttagctcagc ggctcgcaga atttcgccaa gtagaaatgc cgatttacat agcggattat
    20281 caagcattgg aaaatgaaat caagaaaata agtgaacaca tcaatgggca gtcaaaaaag
    20341 cgacagataa aaaaagaact atttgacgac tttgcagaag aattagtaaa ggatgtagaa
    20401 gaattcttga tcaactatcg cgcattttta cgtaaagatt ggcaatccat cgtatatgaa
    20461 gaagtgccag tacatcctaa tgcttatttt gaaacattta tttccgtgtt gagaaagaca
    20521 aaacgtcaac gaacaccaga agtcactgct cagatggaat attttgcgaa aaaccaacga
    20581 ttcatccaac caaaaatcta tctatttatt tggaagcaat attgtcgcta tggttttaca
    20641 gaagggttct tcgacagcat tcctcggata gtcagaaatg gttttttaaa agtattgcgt
    20701 aaagaacgag aagctataca atctgctgat aagaaagacc gtacagtctc aaaagaaaaa
    20761 tggcaactag tagtgaaaga tatccaatct ggaaatgtgc aagagacaat cgacaaatgg
    20821 aaaggaaaac attatcgttt tactgaagct cagaaacaag cgttggaata ttatcagaag
    20881 ttagaagaat cactgcgttt caatgatgag gcaagaaagt atctgaagga attactataa
    20941 aaaatagagg ctgggacaga agcgtttaac tccaagaaat aagaa
//
LOCUS       DACECY010000230        12930 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.guided.077, whole genome shotgun sequence.
ACCESSION   DACECY010000230 DACECY010000000
VERSION     DACECY010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.guided.077"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1880)
                     /locus_tag="IWO38_003061"
     CDS             complement(<1..1880)
                     /locus_tag="IWO38_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0315054.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GD"
     gene            complement(1877..2749)
                     /locus_tag="IWO38_003062"
     CDS             complement(1877..2749)
                     /locus_tag="IWO38_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0315055.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3091..3963)
                     /locus_tag="IWO38_003063"
     CDS             complement(3091..3963)
                     /locus_tag="IWO38_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0315056.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4091..4660
                     /locus_tag="IWO38_003064"
     CDS             4091..4660
                     /locus_tag="IWO38_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ0315057.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            4727..5464
                     /locus_tag="IWO38_003065"
     CDS             4727..5464
                     /locus_tag="IWO38_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0315058.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5695..6963
                     /locus_tag="IWO38_003066"
     CDS             5695..6963
                     /locus_tag="IWO38_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ0315059.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7450..7914
                     /locus_tag="IWO38_003067"
     CDS             7450..7914
                     /locus_tag="IWO38_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0315060.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            7898..8527
                     /locus_tag="IWO38_003068"
     CDS             7898..8527
                     /locus_tag="IWO38_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0315061.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            8881..10359
                     /gene="msr"
                     /locus_tag="IWO38_003069"
     CDS             8881..10359
                     /gene="msr"
                     /locus_tag="IWO38_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0315062.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10406..10852
                     /locus_tag="IWO38_003070"
     CDS             10406..10852
                     /locus_tag="IWO38_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ0315063.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11051..11566)
                     /locus_tag="IWO38_003071"
     CDS             complement(11051..11566)
                     /locus_tag="IWO38_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0315064.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11683..12884
                     /locus_tag="IWO38_003072"
                     /pseudo
     CDS             11683..12884
                     /locus_tag="IWO38_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         4029 a         2381 c         2641 g         3879 t
ORIGIN      
        1 ttgtcgccat ttgtgaatac aacgaccata tccgtgtttt tgcctgcaga ttgaatagcg
       61 gcaagatcaa ctaaagcaat caattgacct cttgcgactt tttgaccttc ttctacatat
      121 aaagtgaatg gttcgccttt taagtcaaca gtgttgatgc ccatatgaag caagacttcg
      181 ataccagcat ctgtttggat tccgacagca tgtttcgttg ggaaaatgtt tgtgatcgta
      241 ccagcgattg gagagaagat ttctccattt tctggtaata cagcatagcc gtcacccatc
      301 attttttccg cgaatacatc atctgaaact tcagtgattg gaatcagttt cccatttgcg
      361 atagcgtaaa tatcttgttc gatcgtttca ccggcaaatg tttctgtttt aaccacggtg
      421 tttgtttgtg caggtcgtgc tgcagcacca ccatgtagtt ttgccatctc atctgcaacg
      481 aattgtactt ctgtacctac gatgacttgc gcattttttc cgtcgatgat cttcatacca
      541 ggtacgccag tggctttgat tttattttga tcgatcaggc ctgtatcttt gacttgcaaa
      601 cgaaggcgtg tagtacagtt atcgatcgaa gttacattgt cggctccgcc taatgcagcg
      661 tagattttgc ttgcttgtgc cgcatattta ttgtctgaat tagtcgttgc agtatcgtca
      721 tctgaaagat ctgcttcctc gcggccagga gtcatcagat ggaatttttt gatagcaaag
      781 ttaaagccaa agtagtaaat cactgctgtt actaatcctt ggacgatcaa catcaatggc
      841 tgattcgcaa tcggattttt caaagatagg aagtaatcga caaatccggc actaaaggca
      901 aatcctgctg tccaatggaa gaatgcactc actgctaaag aaatcccagt gaaaattgca
      961 tgcaagacat ataatggcca agcaacgaac ataaatgaaa attcaagtgg ttctgtcaca
     1021 ccagtaaaga atgaagcaaa agctgctgct agcatcaagg aagctgtttt tgctttgttt
     1081 tctggacgtg catttctgta aatagcatat gctcctgctg gaagcccgaa catcatgatt
     1141 gggaaaaatc cagcttgata cattcctgtt acgccttttg ttccttcaga agccaaaaat
     1201 ttaccaatat cattgattcc tgctacgtca aaccagaaca cggagttcat tgcttgatgc
     1261 aatcctgtag ggatcagtaa acggttgaag aagccataaa gacctgcacc accccagcct
     1321 aaactcaaaa tgaatttagc aaaagaaact aaagcagagt aaaccggagg ccatacgagc
     1381 agtagcactg cactgatcag caacatgaca aaggcagcca agattggcac tagacgcttg
     1441 ccgctaaaga aagataaagc catcggtaat tttgcttcgt ggaaacgatt ataaagagct
     1501 gccgcaatca atccggcaat aataccaata aatacgttac tgatctgagc aaaagatgga
     1561 tcaactgatt ctggtttgat attcaataaa gtagctactg aatcagtttt taaaacattt
     1621 actggaactt caaatgcgac caaacctgcc aaggcagcag cacctgattt gtccactgac
     1681 atccctagtg ctaacccaac ggcaaacagc aaaggtaatt gacctaaaac tgcgttaccg
     1741 ccagcttgta aaaaatgagc gacaatgtcg ttactataag atgcccacca gtttccgatc
     1801 cctactagta aagcagcagc aggtaagacc gcaactggaa gttgaaggga acgtcccatt
     1861 cgttgtagat atgctttcaa tttttttcct cctcatgatt gttaacattt gtttgtacga
     1921 ttgcgcttct taaacgctcg atatggatgg ccaagtaacc cagttcttct ttcgtgatcg
     1981 cgagctggta ttctttggtc agcaacacac ggattttttt agaaatctta tagctttctg
     2041 ggtattttcg tttgaccatt tctacgattt ccgtgtctaa aacagcatat tgttgcgcat
     2101 gggttcgttg tagaagcagt cgtaaatgat tcaccagtct tgaataattc agtgaaaatg
     2161 gttctgaatg gatatctgtt tccaattctg attccactaa atggatgatg tcagatacaa
     2221 tcgtcacttc acggatactg cgatggttat tgttccgacc gcttcgtccg ctgtgaatat
     2281 ggattgcgat atatcctgct tcgtcataag tatatggaat cccaagctct tttgtcagat
     2341 actctactgc ccattgagca atactgaatt catcactgta aagcacttcg atttctctta
     2401 aaagtttgtt gcggatgacg ataccgttat taatgttctc cgcagaaaaa gcaatgtggt
     2461 cggtcaaagc gatcaataca tgttcattca acttttccat caaaacagtt tccgcatgat
     2521 caatgatcgt ttctgctgca aataaaaagc gttcatcaat ctgattaagt aaattttgca
     2581 acttgatttg accttctggt tccatgacaa atatccgttc gacatcacga tcaaaaatca
     2641 aatcatttct ttttctgtca aatccaatcc cttttccaat agctactttt tcctgtccac
     2701 cgtcatctac caatactgca ttttgattaa agatcttttt gattttcatg cgcgcaccct
     2761 ttttgaagat ttggttttca cgtttgctag accagttgat tgcggccaaa caaaaataga
     2821 cacgaaaaac ctcctaggga aaccatgttc ctcagagatt tttcatgcct agtatgatct
     2881 agtcacatga aacgaattat tgttaacgta atcaatttta tatagacagt tatgttttgt
     2941 caattctttt tttataatta tagtaattta ctggaattat cagaaataaa aggaagaaat
     3001 aaattaagaa acttatgtta tttaatcgtt ggatcttttg cttatcttat tcgttgttaa
     3061 aaagctgaaa gcgaaggaaa aaaactctac ttaactgtat ttctccgtta ggacatctaa
     3121 aaatttacga gcagcaggag aaagaggctg gtcttttttc caaatgagat tcagtgaagc
     3181 ttgtaatggc ggatcgagtg ggatgaatat tttttccgtc ccataagtat tgatgatccc
     3241 atctagagct aatgcaccaa aagtcccgtt atcgaccatc aaagaagcat tgtacaataa
     3301 gttatatgtt ccgacgatcg ataactgatc tatgctgaaa cctagccatt cagctagttg
     3361 actgtccact gacgtttgcc gggaaatgat caaaggaaca gttttcagtt cctgcggatg
     3421 gatgacggtt tgctttgcta aaggatgatt tcgatgtatc aaaagtcccc aacgatcttg
     3481 ggctggcagt cgaaatgact ggtatttttg tttgtttgtc ggttcgatta cgagtccaaa
     3541 atcaagtaat ccgctatcca gtttgtctat gatatcatct gcattcccgc tatataaatg
     3601 aaactggata tcaggatgcg ccttgatcaa cgaatgaagg ctatcagaaa tgaaagagat
     3661 tgctttagtt tcgcctccgc caatgtagat ttctccagag atgattgcgt catttgttaa
     3721 attgttcgcc gttttttcta ccaacgacaa gatttctttt ccttttgaat aaagatatcg
     3781 accagaatcc gtcaactgta tctctcggga gccccgagtg aaaagtgtca caccaagttc
     3841 ttcttctaaa tctttcagtt gttttgagag agtaggttgc gtgatatgca gagattttgc
     3901 tgctcggctg atattttttt ctctagctac tgctaaaaaa tacgtcagta tacgaatatc
     3961 cattttttcc ctcctgtttt taaaaaagca taccactaag tatagttaaa aggaatagtt
     4021 aagtatgttt tataagtatt agacatgata agccgtccgt tataaagtaa ggataaaagg
     4081 agggagcaaa atgacaagcc tcatcgaaaa acaaatcgtc aataaagaga ttcgagtaac
     4141 ggatccgctc tttgaagtga tccatcagat ccaagctgaa aacgaagagc ctttagcagc
     4201 gctgaatact gggtaccatg aaccagcaga tatccgcaaa cggctagaaa atatcattag
     4261 tgacaaagta gatgatacgg tgactgttct gctgcctttt tatacagatt tcggcaaaca
     4321 tattagtata gggaaaaatg tctttatcaa tcgtcaagtg atgttcgttg acttaggtgg
     4381 catctgttta gaagattcag tattgattgg tcctcgtgtc aacctcatca cggtgaacca
     4441 tctaaccgat ccaaaagaac gaagaggctt gtcagtaaag ccaatccaca tcaagaaaaa
     4501 tgcttggatc ggagcaggag caactattct gccaggcgta acgattggcg agaatgccat
     4561 tgttgcagcg aatgcaactg ttacgaaaga tgtcccagac aataccatcg ttgcaggaat
     4621 ccctgccaaa atcgtcaaac ctgtcgaacg gattatttga acagtaagat gaaataagag
     4681 atagtgagta taagcaagag atttttcaaa acaggaggag aatagaatga atgatttatc
     4741 agaaaaagtg attgtcatca tgggcgcttc aagcggaatc ggtgaagcaa caacgaagct
     4801 attagcagaa aaaggggcta aactagttat agctgcacgc cgagaagatc gcctaaaagc
     4861 aataaaagaa tctttaccag aagctgagct atatattcaa acagcagatg taagagattt
     4921 tgcacaagtc caagcagtta tcgatttagc gatggaaaag ttcggtcgaa tcgatgtatt
     4981 gtacaataat gcaggaatca tgccaaccgc tccattagtt gaaggtcacc gtgatgaatg
     5041 gcaaaatatg cttgatatca acatcatggg tgtgttaaat ggaatttccg ccgtacttcc
     5101 aatcatggaa aaacaaaaat ctgggcatat catttcaact gattctgtgg cgggacatgt
     5161 tgtttatcct gattccgccg tttattgcgg aacaaaattt gctgtccgtg caatcatgga
     5221 aggattacgt caagagcaac ggcaaaataa catcaaatct acgatcatct ctccaggagc
     5281 tgttcaaact gaattatatc aaaccatcag cgacaaaaag gctgctcttg aacttcatga
     5341 agcgcaaaaa gaatggggat tgacctccga agatatagct tctgctgtag catttgcgat
     5401 agaaacgcca gaccggatgt ctgtcagtaa catgattatc cgtccgacga cacaggaagt
     5461 ataatccata acggataata tccaaagaga attacttgtt aaatagttat tcaaacgacc
     5521 atcaatttaa ggcgtgaatg gagaagaata ttcttcattt acgtcttttt atgtagaaaa
     5581 agcaaattta tcaaaaagaa acaaatgtta tttaaatatg gacattgact tgcttttact
     5641 tttttagtta cactgttatt taggaaaagt ttcataattc aaaggagaaa aaagatgaaa
     5701 gcaaagaatt tattatttag cgttattctt ttaggaaatc tagcagttgc caacacaaca
     5761 gtgctagctg aagaatcgac agaaactgtc gcaactcaga catcgagttc ttcagaaaca
     5821 ggaattgaaa acgatacaga gagtactttg cagtcagcta ctgtctcaga aaatacaatt
     5881 tcaaccgaag aaccaacaac tgaaacaaca gaacaaacac aaaccgaaga cagtacatca
     5941 gcagaagctc agaacaatcc agagaatgca gaagaaatca cgaaccaatt gacagatggg
     6001 caaacattga cacataccca tcaagcagat ttgtctgaat tcgaaaacta tactggctat
     6061 gctttttatg ctcgctcatc tgctaattct caacaagcgt tcattgatag tattgcctca
     6121 actgcacaaa gtttggcaag tgccaatgac ttatatgcat cagtcatgat tgctcaagcc
     6181 atcgttgaaa gtggctgggg gaacagtact ttggcttctg cccctaatta caatctattt
     6241 gggattaaag gaagctataa tggccaatct gttacgatgc ctacttcgga atatgtgaat
     6301 gggcaatgga ttacggtgaa tgccgctttt agaaaatatc cttcgtacaa agaatcattg
     6361 caagataacg taacggtatt gaagacgact tcttttcagc caggcgttta ctactattcc
     6421 ggtgcatgga aaagcaatac gaactcttac aaagatgcta cggcttggct aacaggtcgt
     6481 tatgccactg cgcctaatta tggttctaca ttgaacaatg taatcgaaac ctataatctg
     6541 acacagtatg atacagcacc aacttcaact tctccgatgt atcgtctgta taaccgtcat
     6601 acaggagaac atttatatac attgaatgcc ggagaaaaaa attatctgcc aactgtaggc
     6661 tgggaatatg aagggattgc ttggcaagca ccaaacagcg gacaacctgt ctaccgttta
     6721 tacaatcctt acagtggaga ccatcattac acaatggcac aatctgagat caactcttta
     6781 acaaaaatcg gttggcgtta tgaaggtcta agtttttatt ctggtggttc caagccaatt
     6841 tatcgactat ttaatccaaa tgaaaaaact ggtacccatc actatacctt gagtgctaag
     6901 gaaagagatt ggctaacacc gatgggctgg agatatgaag gaataggatt ttatggttat
     6961 taaaaagagg tagtgaaaaa gctgccctgc atcaagtagt aagaacagcc aaacaaaaac
     7021 agattttcat gttttttgtt tggttgaact tattaccgca gatgcaagtt tttgaacacc
     7081 gtttactcaa aaaaggttgt gacaacatct tgtcataact tttttattac tgtttaagca
     7141 tactgatcca atttctaata ttcgacggga ttcttgcaaa aggcgatttc ggaaattttt
     7201 ggcggtgtaa aattttctac tgcagaagag ctatcttcta tagtttgata agaatcaata
     7261 atcttggttt tagcgttgtg ctttctttat cattttttcc gttcattcta caaaaaattt
     7321 cttttctcta tacttttcta ttgagagcgt tttttttatt acacagctga ttctttgact
     7381 tttttctttt tttattctat tattgacaag tcaatcgttg acgagtcaat tattagaaag
     7441 gaggaaatta tgtctttaga agagattagt caccttttat atcaaatcaa attagccgat
     7501 caaaaaatcg tccagatctt tgaagaaaat atcggtataa gtcttactag atacgagatc
     7561 atgatgagac tgaaagaaga aaatccattg ctacaaagca gtttgcaaga aaagttgcag
     7621 attgatcaag gagcagttac tcgtcatctg aaggtattgg aagcaaatgg ctatgtttcg
     7681 cgtcagagaa atcctcagaa caaccgggaa gtgtatgtag cgctaacaga aaaagcccag
     7741 aagaaaatcg agcattgtga aatggaccat aaagatacag gttccgtgct atccccggct
     7801 ttcagcgatg tagaaatgaa acaatttcta acacttttag ataaattgaa tacccaactt
     7861 tgcaatataa caacagaaac gaaagaggtt aataataatg gaacaaaaaa atgatttttc
     7921 aacaattatg actggtagaa gatccgttcg cgtatatgat gaaacataca aaatcccaca
     7981 cgaagaaatg ctggaaatga tcaaagaagc gacgactgca ccttcttctg tcaatatgca
     8041 accatggcgc tttgtggtcg ctgaaagcga ggaagcaaaa gaaatcttgc gtcctttgat
     8101 ccgtttcaat acgagacaaa atgatacttc ttctgcaatg gtcatgattt ttggcgatat
     8161 gcaatgttac gaatatggcg aagaaatcta taaccaagct tacgaatcag ggaagatgcc
     8221 aaaagaagtc agagatcaac aattagctgc gatcattcct tattacaaga gtttttcaag
     8281 agaagaaatg aacgatgtag tcaaagtcga ttctagctta gcggctatgc aattcatgtt
     8341 agttgcgcgt gatcatggct atgaaacaaa tccaattggt ggattcgaag cagatcagtt
     8401 ggcagaggca tttggtttgg ataaagaccg ttatgttcca gttattattt tatcaatagg
     8461 aaaagcggta gaagaaggtt acgagtctgt tcgtttagca ccagaaaaga tcactacgtt
     8521 cgaataaaag gttttcagtt actattcagt agctattgct ttttttgaat cgtgtgtggt
     8581 aacctaataa aaaaggaggt cggaaaaatg actgcatcga tgaaattacg tttcgaactt
     8641 ttgaataaca actaaataaa ttcaaaagac atgtataaac aacaatttca tggatggagt
     8701 atgtccattt tttcgaacga cagtagaata ggtgaaagct atgacagatt tggtgattta
     8761 gtcatagtta tttcgttcag agtaggaaaa gatgtgatgc agtttttttg caaacagatt
     8821 tttctgctct tttttagtat cgataattgt tgttttacat gaaataggag tgtaaaacat
     8881 atggaaaatt tagcagtaaa tataacaaat ctacaagtca gctttggcaa ccagctagaa
     8941 ctatctattg attctcttcg tgtctatcag caagatcgta tagggatcat aggagaaaat
     9001 ggtgtcggta aatcgacttt gctcaaacta atagccggtg aactttttcc cgatcatggg
     9061 aaaatccaaa cagagatcac cttcaactac ctgcctcaat taacctatct cgctgaagca
     9121 aaggacctaa atttggaatt agccagtcat ttccagttaa gactggaaga aacttcagag
     9181 cggaaatgga gcggagggga agaacgaaag atcgagttga tacgtcttct ttcttcttat
     9241 gaacaaggga tgcttctaga cgagccaaca acccatctag atagaaaaag tattgatcga
     9301 ctgattgaag agcttcgtta ttattatggt acgctggttt ttgttagtca tgatcgctat
     9361 tttctagatg agctagcatc gaaaatctgg gaagtaaaag acggagaaat ccgagagttt
     9421 tcggggaatt atagtgccta tctcactcaa aaggaattgg agaaaaagac tcagctacga
     9481 gaagcagagt cgatcatgaa agagaaaaaa cgattggaaa aatcgatcca agaaaagaaa
     9541 aaacaagcgg aaaagttgga aaaagtgtcc agtaaaaaga aaaagcaaca aatcagaccg
     9601 gatcggttgt cttcctctaa acaaaaagac agtgtacaaa aagccatcca aaagaatgcg
     9661 aaaacattag agagaagact ccaaaaaata ggagaaacaa ccaagccgca gcagatgaaa
     9721 caaatccgtt ttccagtacc aaaatctctt gaactccaca gccgctatcc catcatggga
     9781 caaaatgtcc aattggaaag aagtgggaga acattactgg taaatggtga ttttcagttt
     9841 tctttaggta aaaaaatcgc gattgtcggt gaaaatggtt caggtaagac aaccttatta
     9901 gaacatatcc gcaaacaagg agaaggaatc cttctctctc cgaaagtaag ctttcaagta
     9961 tatcagcaaa agggttatca aatgacatct gaagaatcca tcattcgttt tgtcatgaga
    10021 caaacagagt tttcagaatc gcttgtccgt agtttgctga atcacttagg gtttgctcag
    10081 gaaactttga cgaaaccgtt atgtacatta agtgggggag aagcgacccg tctgacgatt
    10141 gctttgcttt ttactaagcc aagtaatgtg ttgctgttag atgaaccgac taattttatt
    10201 gatatggcaa cgatcgaagc tttagagaag ctaatgcaaa tatatccggg aacgattttg
    10261 tttacttctc atgattctta ctttgtcgag cgtacggctg atgaagttta tgaaataaaa
    10321 gggcagaaaa taaaaaaagt acttacgaga aatttttaaa tgtgctacga tgattttcgt
    10381 gacaaattta aaggagtggt acagattgcc tacaaataaa aaagaaggaa taatttttac
    10441 aacaatgatg tgttttttga tggtcttggg gatgagcgcg tataatttat ggctgcatca
    10501 agatcttcat ttatcaagtt tgttgattgg tctagtgcct ggattcgtgg tggctttcat
    10561 tttggatgtc ttcatcatcg gtgtgctggc gaaaaaaatt gcctttaaac taccagtaaa
    10621 tccagaaagc aaggtccaaa tgatcttaac tatttcatgc ttgatggttt taggaatggt
    10681 gacatgtatg tctttattcg gtgtgatcat ggaacaagga atacctgatc aattattctc
    10741 tgcttatatg aatgcttgga aaatgaatgt gattgccgct ttaccgttgc agttgatcat
    10801 cgttggcccg ctttcaagaa gtgtgctaaa agcaattcaa agtaatgaat gatggaacac
    10861 tgttatttga aaaaaggcct tggcatgctt tttgtcatag tctcttgaat gcagttctag
    10921 aaaaatagtg atccgaacga taggttttgg cctgtcgttc ggatcgcttt ttcttttcca
    10981 actgtattgc tcttttattg aagataattt gctcttcaat agatatttct agaaaggttc
    11041 tgtttatccc ttactcaaaa aactgctgca tttgttctct tacttgctga caccattgga
    11101 gttcctgctg acaaagcata attctgcgct ggatcacaac ataacgtcca aattcttgtt
    11161 tcttgtctag cgagaggtca ttcaattttt tttgattttt ttgcatgatt ttgttcagct
    11221 gttgttctct agtgaatagc agtccagtag ctgttgtttt atcgagcgtc gaaaaagcat
    11281 agatttttgc tagaaattca tctttttgcg taggagcggg agtttcctct ttgatccatt
    11341 cctctacaag agcacgtcct tcatcagtca agtggtagat ctttttttga gagtgctcgt
    11401 ctttgtccaa aacggtcaca taaccctgct ggtctaactt ggcaagcgtt gtatagatct
    11461 gactgtgatg ggcttcccaa aaaattgtga tcagctgctt caattcatac ccagaacgtg
    11521 gtttggataa aagcacgcat aataatatat aggctaaagt attcataaaa atcctacctc
    11581 tcgtcttatt ttctttatta tatcacagta aagtttccac gttttacatg tgcatattta
    11641 catgtaaaat aaatcatgta tgatgctaat agagagaaag ggatggaaat attggagaat
    11701 caaatagata tacaagaaca aacaaatgaa gaaacaaagg ggaaaagttc agcgctagtt
    11761 gtagcagcta cctgtatgat cgcttttatg ggagtgggtc tagtagaccc aatcttaaaa
    11821 acgattgctg cacagttaaa cgctacgcca gctgaaacga ccctgttatt tactagttac
    11881 atgttagtca ctggtgtggt gatgctattt acaggtttct tatctagtcg gatcggtttg
    11941 aaaaaacatt gatgacagga ttgtttatca tcgttgtatt cgctggtctt ggcggattat
    12001 ctgggaatat cggaatgctg attgggttac gtgccggctg gggtgtcggg aatgctttat
    12061 ttgtctctac tgctttagca acgatcgtca gtattttagt tggtaacaca gaaaaagcaa
    12121 tcatgatgta tgaagcagca cttggctgcg gaatggctgt tggccctcta gtcggtgggg
    12181 tcttgggcag tggttcatgg cgttatccat tttttggggt agcatttttg atgttttgcg
    12241 gttttatagc cctagcggta cttttacctg aaactgagaa accaaagaga aaagtaggcc
    12301 tttgggaagg tgtcaaagcg ttaagcaatc ataaacttcg ttcagttgga ctaatcgcat
    12361 tgttatataa tttcggattc tttactttac ttgcgtatgc gccattttta ttgattggct
    12421 attctgaatt agaagtcggt tttgtatttt ttggctgggg tgtgcttctg gctattgctt
    12481 cgattttcgt tgcaccggct cttgagcgga agtttacgac ttatcggaca attttagcag
    12541 catttgtctt attcattatt tgtttgctgt tattaggagc tggaacttct atgccagtcc
    12601 ttgtaccgat cagcattgtg ttagcaggat ttttccaagg aattatcaat acactgttga
    12661 cgacgattgc tatggaaata ccaggtctgc aacgaaacgt tgcttcgtcc agctatagtt
    12721 ttgtccgctt tttcggtgga gctttagcac catttattgc aggtgtgatt ggtgaacata
    12781 ttggtgaaag ctatgcgttt tattttgcag gtgccattgt tcttatcagt gtatgttgta
    12841 tcatttatca ccgtaattat tttgctacaa atgaagaaga ataagaaatc agaggttgtg
    12901 aaaaagctgt cctgcatcaa gtcataagaa
//
LOCUS       DACECY010000231         7959 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.guided.102, whole genome shotgun sequence.
ACCESSION   DACECY010000231 DACECY010000000
VERSION     DACECY010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.guided.102"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            128..1564
                     /locus_tag="IWO38_003073"
     CDS             128..1564
                     /locus_tag="IWO38_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0315065.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="IWO38_003074"
     CDS             1753..4200
                     /locus_tag="IWO38_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ0315066.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="IWO38_003075"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="IWO38_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ0315067.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="IWO38_003076"
     CDS             complement(5690..6793)
                     /locus_tag="IWO38_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0315068.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="IWO38_003077"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="IWO38_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ0315069.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2664 a         1359 c         1729 g         2207 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacatat ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattactt
//
LOCUS       DACECY010000232         5781 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.guided.118, whole genome shotgun sequence.
ACCESSION   DACECY010000232 DACECY010000000
VERSION     DACECY010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.guided.118"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(292..663)
                     /locus_tag="IWO38_003078"
     CDS             complement(292..663)
                     /locus_tag="IWO38_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0315070.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            808..993
                     /locus_tag="IWO38_003079"
     CDS             808..993
                     /locus_tag="IWO38_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0315071.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            1936..2595
                     /gene="vanR"
                     /locus_tag="IWO38_003080"
     CDS             1936..2595
                     /gene="vanR"
                     /locus_tag="IWO38_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ0315072.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDLEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFESDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            2598..3941
                     /gene="vanS"
                     /locus_tag="IWO38_003081"
     CDS             2598..3941
                     /gene="vanS"
                     /locus_tag="IWO38_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ0315073.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRNGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            4112..4918
                     /gene="vanY"
                     /locus_tag="IWO38_003082"
     CDS             4112..4918
                     /gene="vanY"
                     /locus_tag="IWO38_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAQ0315074.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            4931..5767
                     /gene="vanW"
                     /locus_tag="IWO38_003083"
                     /pseudo
     CDS             4931..5767
                     /gene="vanW"
                     /locus_tag="IWO38_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
BASE COUNT         1687 a         1213 c         1427 g         1454 t
ORIGIN      
        1 cgcgcagcac gatttttcgc tttctgtttg ccatgcttca ccgtcctttc cattcagggg
       61 tttggggatt tccccaacaa gcaaaacgga cgcggcccgg cagggacggg gcaggccgtt
      121 tttccgggag tggctacact ccctatgctt gctacgttac tgtttctcca ctaacaagac
      181 gtttccaaag ccccaaacta cccgcaaaat agccctctgg aaagaaaaaa gtgcaaaaaa
      241 atagaagcta cgctgtgcag ctcccgctaa aagtaaaaca gaggatgcca gttacacatc
      301 ctccgtttca ttcttcttcg gcttttcaat tagtatggcg ctgtcaatcg cgccctcgat
      361 gatcggcagg tattcttcgg ggcagagttt cagcttgtgg ctgacccttt gccgcagttc
      421 gctttcctcc cgcatgatct ccgggttaaa gtagcgttcc acaggaagcc cgcaggcttt
      481 aataagctgt atgacaaccg gcagactggg aatggccccg tcgttttcga tgttggcaag
      541 atagcgccag tcaatattta ttttctctgc cattgctttg cgcgaaacgc gctttgcttg
      601 ccgggccgct ttcacatccg cgccgaaagt ttcaaagccg ggacaatctt caatattcgc
      661 catatcgcac ctcccactta cattgtatat ttcatactct gcccgtggaa tgttggtata
      721 tgcacctttc ttaaagttta tatttcacat tttgtttctt ttattcttcg agcaaaagaa
      781 ttataatgta ccctgtggag gtgtactatg gactacatga cgttgaaaga ggcaggcgag
      841 aaatggggtg tgcccccacg atggataaac tactattgtg ctgcgggacg catccccggc
      901 gctgtaaaga tggcgacgat ctggctcatt cccaaaactg cggaaaagcc gattgacagg
      961 cgtacaaaga aaggaaaggc ggttactgaa tagtataatg aaaccggcgc attttggata
     1021 tggttttgaa gagagagaaa gcatggtatt ttgtcacgga aaaactataa gatgatagat
     1081 ggcaggctgc tccagaccga taaaaagtat tctcatctga aacagaaaga aaagatttac
     1141 acatggatgt atgaagaagc gaaactttat catgacagaa atggaaaatg cccgaaacaa
     1201 agacgaggaa gctgctgctg tatcagccgt atatggtaga attgaaaagg ccggcatctg
     1261 gattctttat ggtgaagtct ccagacattg gataactgct ggtctaatgc gcctttgagt
     1321 atggggtgat tgattttaat accggggagg tacggctttt tgaggaaaat attctgggat
     1381 ttacatttgg cgattattac atttcctgta actgcaagga cacggatgat gaagatatga
     1441 ggaatataga cttgctgatt ttctactgtc ttgaacggta ttaggactga atctgctgcg
     1501 cggagtgcgg caagagcgac cggagggagt acacacaggc cctgcctgtg tgaaaagcca
     1561 taccgcaagg tgcattcgca aaaaagaggc cgcgcatttt gctgaggctt ggggtgtccc
     1621 caacaagcaa aacgagaacg ccgcaggcgg gcaagtggtg gatcagaata tccgtaaagg
     1681 atattctgat ccattgcttg ccagtattat cgggatgttt tgacaaccgc tgtaatcgaa
     1741 acatcccaaa cattaagaag gtgttaatta ttattatctt gatttaattc gtttaaacca
     1801 ataatataga tacatacacc atgaaaagaa gcgagtatcc caatgacgga tgattaaaat
     1861 ccgaaaagaa atcgctttga aactacaggg aaactacaga ctgttatgtt atcttcttaa
     1921 aatggaggga tttttatgtc gatacgaatt ctacttgtcg aggatgatga tcatatctgt
     1981 aatacagtaa gggcgtttct ggctgaggca ggatatcagg tggatgcctg cacagatgga
     2041 aatgaggcat acaccaagtt ttacgaaaac acttatcaac tggttattct tgatattatg
     2101 ctgcccggta tgaacgggca tgaacttttg cgtgaatttc gtgcgaaaaa tgatactccc
     2161 attctgatga tgacagccct gtcggatgac gaaaaccaaa tccgggcgtt tgatctagag
     2221 gcagacgact atgtaacaaa gccattcaag atgcagattt tactaaagcg ggtggaagcc
     2281 ctgctgcggc gcagcggtgc gctggcaaag gaaatccgtg tcggcaggct gacacttctg
     2341 ccggaggatt tcacggtact ttgtgacggt acagagctgc ccctgacacg aaaagaattt
     2401 gaaatccttt tgctgctggt gcagaacaaa ggcagaacct taacgcatga aatcattttg
     2461 tcacgtatat ggggatatga ctttgagagt gatggcagca cagtccacac tcatatcaaa
     2521 aatttgcggg ccaagctgcc ggaaaatatc atcaaaacca ttcgcggtgt aggataccga
     2581 ttggaggaat cataataatg gaaagaaaag ggattttcat taaggttttt tcctatacga
     2641 tcattgtcct gctactgctt gtcggtgtaa cagcaacact gtttgcacag caatttgtgt
     2701 cttatttcag agtgatggaa ttacagcaaa cagtaaaatc ctatcagcca ttggtggaac
     2761 tgattcagaa tagcgatagg cttgatattc aagaggtggc agggctgttt cactacaata
     2821 accaatcctt tgagttttat attgaagata aagagggaag cgtactctat gccacaccaa
     2881 atgccaatac atcaaatagt tttagacccg actttcttta tgtggtacat agagatgata
     2941 atatttcgat tgttgctcaa agcaaggcag gtgtgggatt gctttatcaa gggctgacaa
     3001 ttcggggaat tgttatgatt gcgataatgg ttgtattcag ccttttatgc gcgtatatct
     3061 ttgcgcggca aatgacaacg ccgatcaaag ccttagcgga cagtgcgaat aaaatggcaa
     3121 acctgaaaga tgtaccgccg ccgctggagc gaaaggatga gcttggcgca ctggctcatg
     3181 atatgcattc catgtatgtc aggctgaaag aaaccatcgc aaggctggag gatgaaatcg
     3241 caagggaaca tgagttggag gaaacacagc gatatttctt tgcggcagct tctcatgagc
     3301 taaaaacgcc catcgcggct acaagcgttc tgttggaggg aatgcttgaa aatatcggtg
     3361 actacaaaga tcattctaag tatctgcgcg aatgcatcaa aatgatggat aggcagggca
     3421 aaatcatttc cgaaatactg gagcttgtca gcctgaatga tgggagaatc gtacccatag
     3481 ctgaaccgtt ggacataggg cgcacggttg ccgagttgct gcccgatttt caaaccttgg
     3541 cagaggcaaa caaccagcgg ttcgtcacag atattccagc cgggcaaatt gtcctgtccg
     3601 atccgaggct gctccaaaag gcactatcca atgtcatatt gaatgcagtt cagaacacgc
     3661 cgcagggagg cgaggtacgg atatggagtg agcctggtgc tgaaaaatgc cgcctttttg
     3721 ttttgaacat gggcgttcac attgatgata ctgcgcttcc aaggctgttc accccattct
     3781 atcgcattga tcaggcgcga agcagaaaaa gtgggcgaaa cggtttagga cttgccatcg
     3841 tacaaaaaac gctggatgcc atgagcctcc agtatgcgct ggaaaacacc tcggatggcg
     3901 ttttgttctg gctggattta ccgctcacat caacattgta aatatttaaa acttaaatga
     3961 ttttgaccga caggtataac cctgccggtc tttttttgct gctacaggaa aactacagac
     4021 tggctacagg gaaagtacag atgcgcttgc cataataaca atcgtaccag ccacaaatcg
     4081 tagttttatt gcaaaggagg cattcgatca aatggaaaaa agcaactatc attccaatgc
     4141 ggatcatcac aaacggcata tgaaacaatc cgtggaaaag cgggcttttc tatgtgcgtt
     4201 cattatttcg ttcacagtct gcacgctgtt tttggggcgg agactggctt ccgtattgga
     4261 ggcaacacag ataccgccca tccctgcaac tcatacaggc agcagcactg acgtagtgga
     4321 gaatttggag gaaaacgctc ttgccaccgc caaagaacag ggagatgaac aggaatggag
     4381 cctgatttta gtgaacaggc agaaccccat ccccgcacag tacgatgtgg agcttgagca
     4441 actatcaaat ggtgagcgga tagatattcg gatttctccc tatcttcaag atttgtttga
     4501 tgccgcaaga actgatggag tttacccgat tgtcgcatcc ggataccgaa caacagaaaa
     4561 acagcaagaa attatggatg aaaaaattgc cgaatataag gcgaaaggct acacctctgc
     4621 acaggctaaa gcggaagcag aaacttgggt ggccgtgccg ggaacgagcg agcatcagct
     4681 tggtcttgct gtggatatca atgcggacgg aattcattca acaggcaacg aggtttatag
     4741 atggctggat gaaaacagct atcgttttgg ttttattcgt cgctacccac cagacaagac
     4801 agagataacc ggtgtgagca acgagccgtg gcattaccga tatgttggca tcgaagctgc
     4861 cacagagatg tacaaccaag gggtttgcct tgaggaatat ttaaaaccag aaaaatgaga
     4921 aaaggatgtt atggacagaa aaagattgac acagcgtttc ccgttcctgc ttccaatgag
     4981 acgggcgcag agaaaaatgt gcttttatgc gggaatgaga tttgacggct gtcgctatgc
     5041 gcagacgata ggggaaaaat cgctttccca tttgctcttt gaaacggatt gcgcattata
     5101 caaccacaat accggatttg acatgatata ccaggaaaac aaggtgttca acttaaagct
     5161 ggcagcaaag accttaaacg gcttattgat aagaccgggg gaaacctttt ctttctggtg
     5221 gctggtacgc catgcggaca aagatacccc ctataaagac ggccttacgg tgaccaatgg
     5281 taaactcacc accatgtcgg gcggcggtat gtgccagatg agcaatttac tattttggat
     5341 gttcctgcat acgccattga caattatcca gcgcagaggt cacgaagtaa aggagtttcc
     5401 agagccaaac agtgacgaga ttaaaggggt ggatgcaacc atttcagagg gctggattga
     5461 tttaaaagtg cgaaacgata ccgactgcac ctaccaaata tgagtgacct tagatgatga
     5521 gaaaatcatc ggtcaggtgt ccgccgacaa agagccccaa gcattataca aaattacaaa
     5581 tggcagtatc cagtatgtcc gtgaaagtgg cgggatttat gaatatgccc aggttaaacg
     5641 gatgcaagtt gccttaggta ccggggaaat aatagattgc aagctgcttt atacaaacaa
     5701 atgcaaaatc tgttatcccc tcccggaaag tgtggatatt cagtctaatg gtgataagtc
     5761 aagatgaaat ttaagatttt c
//
LOCUS       DACECY010000233         3604 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.guided.141, whole genome shotgun sequence.
ACCESSION   DACECY010000233 DACECY010000000
VERSION     DACECY010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.guided.141"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..164)
                     /locus_tag="IWO38_003084"
     CDS             complement(<1..164)
                     /locus_tag="IWO38_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ0315075.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELC"
     gene            394..891
                     /gene="dfrG"
                     /locus_tag="IWO38_003085"
     CDS             394..891
                     /gene="dfrG"
                     /locus_tag="IWO38_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ0315076.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(963..2915)
                     /locus_tag="IWO38_003086"
     CDS             complement(963..2915)
                     /locus_tag="IWO38_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ0315077.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2922..3218)
                     /locus_tag="IWO38_003087"
     CDS             complement(2922..3218)
                     /locus_tag="IWO38_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315078.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(3393..>3604)
                     /locus_tag="IWO38_003088"
     CDS             complement(3393..>3604)
                     /locus_tag="IWO38_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0315079.1"
                     /translation="ELEQEESIRFQNCSLFPLYHGSAKSNIGIDNLIEVITNKFYSST
                     HRGPSELCGNVFKIEYTKKRQRLAY"
BASE COUNT         1115 a          622 c          583 g         1284 t
ORIGIN      
        1 ttacataatt caccatttat tgaagtatac atttctgtaa cttttatttt ttctttttct
       61 gatactctaa ccgaatctcg taaatgtagt actccactat aaaggcgtat atatgcaagt
      121 aagtagttca gttttggagt acaaaatgac aaaatgtact tcaaaatgga ctactttttt
      181 cattttaact tacataccaa attacataca aaatgattta taaaatgcaa ttgaaaatga
      241 taattgaatg aaaatttgtg attttgtggt attatgataa tataacttat agaaaaaatt
      301 gcttattcaa ctaacgggca ggataatgag aaaaggtttt ttgatagaaa taagaaatta
      361 gatggtaaat tagttaaaaa ggctgtgtta taaatgaaag tttctttgat tgctgcgatg
      421 gataagaata gagtgattgg caaagagaat gacattcctt ggaggattcc caaggactgg
      481 gaatatgtta aaaatactac aaagggacat ccgataatat taggtaggaa gaaccttgaa
      541 tcaatcggaa gagccttacc tgacagaaga aatattattc tgacgagaga taaggggttt
      601 acctttaatg gttgtgaaat tgttcattca atagaagatg tttttgagtt atgtaaaaac
      661 gaagaagaaa tttttatttt cggaggagaa cagatttata atttgttttt cccttatgtt
      721 gagaaaatgt acatcacaaa aatacatcat gaattcgaag gagatacttt ttttccagaa
      781 gtgaattatg aggaatggaa tgaggtattt gcccaaaaag ggataaagaa tgataaaaat
      841 ccgtataact actattttca tgtatatgaa agaaaaaact tattgagtta acgggtgcgt
      901 taattgaaga agaacagtaa agataaagca caagagcttg ggattactcc aagctctttt
      961 tgtcatttga aataaattat atctttccaa tcaaattgtt tatctgtaat ccctaatctt
     1021 tgagcaggtg tcaaaacctt tttatccgct gatttaaaag ggcgacaaaa gttgtagtaa
     1081 gttcttagaa ttgtaatcgc aaattgagca tacttcggat tgaagttagc gtaaatatag
     1141 ctcttcttat caccacgagc tgttgttaat ggtctttcaa gagatgatat ataacgtctt
     1201 atctgttgaa taaagctatt ggtcgaatgg tcattaacat ttaatagcat ctttgcgatt
     1261 tcgtttggtt ccagagcaga taaatccgtt ctacaatcaa ctgaataaaa tcctttatcc
     1321 ttagtggcta atggatgttt gatagggtta tcggcatatt tgcgatatct ttctccatct
     1381 ttactaatag cctcttcgtg gaattgatga cttgttagta attcagttag ataatgagat
     1441 gccaaagtcc gtagaaactt tgttttgaat cctctgatat ctccccaatc gagcagttct
     1501 gcttttgctt gtccgaattc ttttaagcat tgttttctgc ttttcgtctt gttaacttgg
     1561 caaataaaat gatgtgcatc gctcaacctt aattctttcg tgaatactct ataaaaggcg
     1621 tttctaatag agaagtcatc atcactaatc attcgccatt cgtcggcatt aaccatttgt
     1681 ttaattaacc aatagtgagc agttgtagta taaggagcat caacatttaa accatcgaca
     1741 aatctactcc tattagtaat tttccctaat tcgtgaagat aagcattcct attttcgcta
     1801 tcgttagcgg atggttcttg tggataataa gaccaatcta acctatcatt tttacgacta
     1861 aaagtattga gatggtcttc tttgaatttt cttgtatctt catttaattc atccattgaa
     1921 atattccaat cataggcaac atcagaacgg aacacatatc ttgataagac ctcagcagat
     1981 acaactatat acgtttgcat attaaggtct tcaaaccccg tatatttctt tgaaccttgt
     2041 cctttcttac ggacgttatt taggtagtag tgcatcttgt ccgtattaag ccacatctcg
     2101 ttaaatttct ttgtttgaag gggttgtgtc tcatatcgtt ctaaaaactc caaacaacgc
     2161 ctatacagcc actccagctt atgatagtag gttccaactc caattcctaa aatatcacaa
     2221 gtacgactga ctgaaaccct tccaacaacc atcttagcga acattggaag aatggtgttc
     2281 ttttgttggt gataagtagt ggtttcttct ctttttggta gaacattagt gaacttctta
     2341 cacgccttac attgatagcg ttgagatttg cccctacttt tccccctttt gtaaaattgc
     2401 ttcggctcat tgaatggggt tgattcttct tccgagcagc cttctttatg aaattgataa
     2461 tctggctcaa catcttggat gctattgatt tctataaggc gtttaatttc ttcaacaaca
     2521 gaccaattag ataaaggggt tactgtacag ttttgcgata cacctctgcc aataggattg
     2581 tcattgcagt acataccttt atgaccttta tctttggaac taccagtcat tttatatcga
     2641 cttggtttcc cttttacatc tttgaatttg tgctgttctt tcccaaaatt acaacaaaac
     2701 ggattgatgc agtaattata ttgaatggga tattcagaac cgttccattt aaaagacgtt
     2761 ggcaagaaca acattttgtt ataccttgtt gcaaatcgtt ttggagttaa taaatcatat
     2821 tgtttagccc tatcttttaa ttcttcgttt gaaatcggaa ttttaacatc tacaatttca
     2881 tcttccttcg tggctaatct tttaagcttg gtcattgtct atcaacccac tctatctttg
     2941 atacccattt tttcaacaat ccgcttatta cttcgcttgt tggcaatcca ttcaataaac
     3001 ttcttatcgt ctaaaatgtt caataagaat tcatccactg cttcgctttc agtacgctct
     3061 gcatattcag cgtattgctt aactatcacc cttacttgct ctgatatttc ccaatccaca
     3121 gacttagcgt tcttattttt tggctcaaga aattgcatag gaaaatgcac ccctttttgc
     3181 ttagtaaatt gtaaccattt taacacacca atttgcattc ctacaataat tatttcacgt
     3241 tcgcattttt atatacattt taacttacat tttgtggtgt aaattaacaa caaacgttga
     3301 tttaacaata aaaaacgagt atcaaattca gttgcaattt gatactcatt taggtttaca
     3361 ttttcacttt ttgtactcca aaactgaact acttaatatg caagacgttg tctttttttt
     3421 gtatattcaa ttttgaaaac atttccgcaa agttcagacg gacctcgatg tgttgatgaa
     3481 taaaatttat tagtaataac ttctataagg ttatcaatcc ctatattact ttttgcactt
     3541 ccatgataaa gagggaacag agaacaattc tgaaatctta tgctttcctc ttgttcgagt
     3601 tcca
//
LOCUS       DACECY010000234         2499 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.guided.154, whole genome shotgun sequence.
ACCESSION   DACECY010000234 DACECY010000000
VERSION     DACECY010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.guided.154"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IWO38_003089"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IWO38_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0315080.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..>2499
                     /locus_tag="IWO38_003090"
     CDS             2039..>2499
                     /locus_tag="IWO38_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0315081.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVG"
BASE COUNT          830 a          383 c          522 g          764 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcgg
//
LOCUS       DACECY010000235         2499 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.guided.155, whole genome shotgun sequence.
ACCESSION   DACECY010000235 DACECY010000000
VERSION     DACECY010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.guided.155"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IWO38_003091"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IWO38_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0315082.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..>2499
                     /locus_tag="IWO38_003092"
     CDS             2039..>2499
                     /locus_tag="IWO38_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0315083.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVG"
BASE COUNT          829 a          383 c          523 g          764 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcgg
//
LOCUS       DACECY010000236         2269 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.guided.164, whole genome shotgun sequence.
ACCESSION   DACECY010000236 DACECY010000000
VERSION     DACECY010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.guided.164"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..331
                     /locus_tag="IWO38_003093"
     CDS             <1..331
                     /locus_tag="IWO38_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0315084.1"
                     /translation="AGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNE
                     VILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVR
                     YMFNKIT"
     gene            429..1802
                     /gene="tet(L)"
                     /locus_tag="IWO38_003094"
     CDS             429..1802
                     /gene="tet(L)"
                     /locus_tag="IWO38_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ0315085.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT          667 a          346 c          456 g          800 t
ORIGIN      
        1 agctggaaca gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata
       61 tctttcacga gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt
      121 gaaaaataat gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg
      181 tagtgattta actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta
      241 ccatgttact accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa
      301 agtacgatat atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt
      361 ttcttgttaa ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg
      421 aattaattat gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc
      481 tttgcgtttt atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg
      541 atattgcaaa tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta
      601 tgttaacctt ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca
      661 aaaggttact cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg
      721 gccattcttt cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg
      781 catttccagc actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta
      841 aagcatttgg tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg
      901 gtggaatgat agcccattat attcattggt cctatcttct actcattcct atgataacaa
      961 ttatcactgt tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt
     1021 ttgatatcaa aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa
     1081 catcatatag catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac
     1141 atatcaggaa agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta
     1201 tgattggagt tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg
     1261 ttccttatat gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta
     1321 ttttccctgg aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata
     1381 gaagaggtcc tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa
     1441 ctgcttcctt tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt
     1501 taggtgggct ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac
     1561 agcaggaagc tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa
     1621 caggtattgc aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac
     1681 ctatggaagt tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa
     1741 tcattgtcat tagttggctg gttaccttga atgtatataa acattctcaa agggatttct
     1801 aaatcgttaa gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg
     1861 aattcaaatc tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc
     1921 tctcttgtat cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca
     1981 ataaaaattt tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata
     2041 aaaattggca agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc
     2101 tgtcccttgc tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag
     2161 agggcaggtt tttttgtttc ttttttctcg taaaaaaaag aaaggtctta aaggttttat
     2221 ggttttggtc ggcactgccg acagcctcgc agagcacaca ctttatgaa
//
LOCUS       DACECY010000237         2255 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.guided.165, whole genome shotgun sequence.
ACCESSION   DACECY010000237 DACECY010000000
VERSION     DACECY010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.guided.165"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..247
                     /locus_tag="IWO38_003095"
     CDS             <1..247
                     /locus_tag="IWO38_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAQ0315086.1"
                     /translation="EALESGKLGGAALDVLEGEDQFVYTDCSQKVLDHPFLSQLLRMP
                     NVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            240..1268
                     /gene="vanB"
                     /locus_tag="IWO38_003096"
     CDS             240..1268
                     /gene="vanB"
                     /locus_tag="IWO38_003096"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAQ0315087.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVHADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            1274..1882
                     /locus_tag="IWO38_003097"
     CDS             1274..1882
                     /locus_tag="IWO38_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAQ0315088.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT          612 a          470 c          605 g          568 t
ORIGIN      
        1 ggaggcgttg gaaagcggaa agctgggtgg tgcggcactg gatgtgttgg agggcgagga
       61 tcagtttgtt tataccgatt gctcgcagaa agtgcttgac catccctttt tgtcgcagct
      121 tttaaggatg ccgaatgtga tcatcacacc ccatacggcg tactacactg agcgtgtgct
      181 gcaggatact acagaaaaaa caatcaggaa ttgtctcaat tttgaaagga gtttacagca
      241 tgaatagaat aaaagtcgca atcatcttcg gcggttgctc ggaggaacat gatgtgtcgg
      301 taaaatccgc aatagaaatt gctgcgaaca ttgatacgga aaaattcgat ccgcactaca
      361 tcggaattac aaaaaacggt gtatggaagc tatgcaagaa gccatgtacg gaatgggaag
      421 ccgacagtct ccccgccata ctctccccgg ataggaaaac gcatgggctg cttgtcatga
      481 aagaaagcga atacgaaaca cggcgtattg atgtggcttt cccggttttg catggcaaat
      541 gcggggagga tggtgcgata caggggctgt ttgtattgtc tggtatcccc tatgtgggct
      601 gtgatattca aagctccgca gcttgcatgg acaaatcact ggcctacatt cttacaaaaa
      661 atgcgggcat cgccgttccc gaatttcaaa ttattgataa aggtgacaag ccggaggcgg
      721 gtgcgcttac ctaccctgtc tttgtgaagc cggcacggtc aggttcgtcc tttggcgtaa
      781 ccaaagtaaa cggtacggaa gaacttaacg ctgcgataga agcggcagga caatatgatg
      841 gaaaaatctt aattgagcaa gcgatttcgg gctgtgaggt cgggtgtgcg gtcatgggga
      901 acgaggatga tttgattgtc ggcgaagtgg atcaaatccg gctgagccac ggtatcttcc
      961 gcatccatca ggaaaacgag ccggaaaaag gctcagaaaa tgcgatgatt acagttcacg
     1021 cagacattcc ggtcgaggaa cgaaatcggg tgcaggaaac ggcaaagaaa gtatatcggg
     1081 tgcttggatg cagagggctt gcccgtgttg atcttttttt gcaggaggat ggcggcatcg
     1141 ttctaaatga ggtcaatacc atgcccggtt ttacatcgta cagccgctac ccacgtatgg
     1201 tggccgccgc aggaatcacg cttcctgcac tgattgacag cctgattaca ttggcgttaa
     1261 agaggtgata agtatggaaa atggtttttt gtttttagat gaaatgttgc atggcgtccg
     1321 ttgggatgcc aagtacgcca catgggataa cttcacagga aaaccggtgg atgggtatga
     1381 ggtgaatcgc atcatcggca caaaggccgt ggcgtttgct ctgcgcgagg cacaaatcca
     1441 tgcggctgcg cttggctatg gcttgctttt atgggatgga tatcggccaa gaactgcggt
     1501 ggactgcttc ctgcgttggg cagcgcaacc ggaggacaat ctcacaaaag aaaaatttta
     1561 ccccaatata gagcgagccg agttgattac aaagggttat gtggcctcac aatccagcca
     1621 tagccgtgga agcgcaattg atcttacgct ctaccacctg gatacagggg aacttgtttc
     1681 aatgggaagt aacttcgatt ttatggacga acggtcgcat catacagcaa aagggatagg
     1741 ggatgcagag gcacaaaatc gaagatgctt gcgtaaaatc atggaaagca gcggatttca
     1801 gtcttatcgc tttgaatggt ggcactataa gttgattgat gagccatacc ccgataccta
     1861 ttttaatttt gccgtttcat aatgaaagta ttggttttgt atcagatttt ggatttgata
     1921 aaatctatgc tggtcatgga caatctttta ctggaaagtg gttaaagcat cttttgtaat
     1981 acattgcaaa ttatagtatt caatctgccc agcggcagaa aagaaaaaaa ccgctgttgg
     2041 gcagaccttt cccacgccca ttttgtattt tgctggcggc ggttttgaga aatcaaggcc
     2101 gccgttcttt tctggcagca aaaggtgtgg cggcccatag gaggatgccg ctatgctgtt
     2161 ttgctattgc catgctctaa tccacttcca ccactcaaaa aagccttgcc cctccatgaa
     2221 ctgaacccca aaagttgaac tatttaatgg actgt
//
LOCUS       DACECY010000238         1970 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.guided.172, whole genome shotgun sequence.
ACCESSION   DACECY010000238 DACECY010000000
VERSION     DACECY010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.guided.172"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            47..439
                     /locus_tag="IWO38_003098"
     CDS             47..439
                     /locus_tag="IWO38_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0315089.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            440..1879
                     /locus_tag="IWO38_003099"
                     /note="aph(2'')-Ia"
     CDS             440..1879
                     /locus_tag="IWO38_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAQ0315090.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          819 a          149 c          337 g          665 t
ORIGIN      
        1 aacattgtat agcattttac acaggagtct ggacttgact gagtttatgg aagaagtttt
       61 aattgatgat aatatggttt ttgatattga taatttaaaa ggatttctta atgataccag
      121 ttcatttggg tttatagcta aagaaaataa taaaattata ggatttgcat attgctatac
      181 acttttaaga cctgatggaa aaacaatgtt ttatttacac tcaataggaa tgttacctaa
      241 ctatcaagac aaaggttatg gttcaaaatt attatctttt attaaggaat attctaaaga
      301 gattggttgt tctgaaatgt ttttaataac tgataaaggt aatcctagag cttgccatgt
      361 atatgaaaaa ttaggtggta aaaatgatta taaagatgaa atagtatatg tatatgatta
      421 tgaaaaaggt gataaataaa tgaatatagt tgaaaatgaa atatgtataa gaactttaat
      481 agatgatgat tttcctttga tgttaaaatg gttaactgat gaaagagtat tagaatttta
      541 tggtggtaga gataaaaaat atacattaga atcattaaaa aaacattata cagagccttg
      601 ggaagatgaa gtttttagag taattattga atataacaat gttcctattg gatatggaca
      661 aatatataaa atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat
      721 agtctatggt atggatcaat ttataggaga gccaaattat tggagtaaag gaattggtac
      781 aagatatatt aaattgattt ttgaattttt gaaaaaagaa agaaatgcta atgcagttat
      841 tttagaccct cataaaaata atccaagagc aataagggca taccaaaaat ctggttttag
      901 aattattgaa gatttgccag aacatgaatt acacgagggc aaaaaagaag attgttattt
      961 aatggaatat agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga
     1021 gcattacttt gataatttca aagtagatag tattgaaata atcggtagtg gttatgatag
     1081 tgtggcatat ttagttaata atgaatacat ttttaaaaca aaatttagta ctaataagaa
     1141 aaaaggttat gcaaaagaaa aagcaatata taatttttta aatacaaatt tagaaactaa
     1201 tgtaaaaatt cctaatattg aatattcgta tattagtgat gaattatcta tactaggtta
     1261 taaagaaatt aaaggaactt ttttaacacc agaaatttat tctactatgt cagaagaaga
     1321 acaaaatttg ttaaaacgag atattgccag ttttttaaga caaatgcacg gtttagatta
     1381 tacagatatt agtgaatgta ctattgataa taaacaaaat gtattagaag agtatatatt
     1441 gttgcgtgaa actatttata atgatttaac tgatatagaa aaagattata tagaaagttt
     1501 tatggaaaga ctaaatgcaa caacagtttt tgagggtaaa aagtgtttat gccataatga
     1561 ttttagttgt aatcatctat tgttagatgg caataataga ttaactggaa taattgattt
     1621 tggagattct ggaattatag atgaatattg tgattttata tacttacttg aagatagtga
     1681 agaagaaata ggaacaaatt ttggagaaga tatattaaga atgtatggaa atatagatat
     1741 tgagaaagca aaagaatatc aagatatagt tgaagaatat tatcctattg aaactattgt
     1801 ttatggaatt aaaaatatta aacaggaatt tatcgaaaat ggtagaaaag aaatttataa
     1861 aaggacttat aaagattgat tatataatat atgaaaagct attataaaag acattagtat
     1921 taaatagttt aaaaaaattc aaaatctttc agagaagctg ttaaaggaat
//
LOCUS       DACECY010000239         1759 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.guided.176, whole genome shotgun sequence.
ACCESSION   DACECY010000239 DACECY010000000
VERSION     DACECY010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.guided.176"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            47..1018
                     /locus_tag="IWO38_003100"
     CDS             47..1018
                     /locus_tag="IWO38_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAQ0315091.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            1011..>1759
                     /locus_tag="IWO38_003101"
     CDS             1011..>1759
                     /locus_tag="IWO38_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAQ0315092.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPE"
BASE COUNT          475 a          369 c          502 g          413 t
ORIGIN      
        1 gaataaaaac tattagacaa atgatatttt acgaggagga gaaccaatga gaaaaagtgt
       61 gggcattact gtttttggat gcgaacagga tgaggcaaat gctttccgcg ctttatcgcc
      121 ggattttcat attatcccta cgctgattag cgatgcgata tcggcagaca acgcaaaatt
      181 ggccgctggc aatcaatgcg ttagcgtagg ccataagtcc gaggtttccg aggcgacaat
      241 tcttgcgctg agaaaggtcg gggtaaaata catttctacc cgcagcatcg gctgcgatca
      301 cattgatacg actgccgccg agagaatggg aatctcggtt ggcacggttg cgtattcgcc
      361 ggacagcgtt gcggattatg ctttgatgct gatgctgatg gccatacggg gtgcaaaacc
      421 caccatgcac gccgtggcgc aacaagattt cagattggat cgtatccggg ggaaagaact
      481 gggggatatg actgtgggag ttattggaac cggccatatc gggcaagcgg tcgtcaaaag
      541 gctgcgggga tttggatgcc atgtgctggc ctatgataac agccgaaaaa tggatgcaga
      601 ttatgtccag cttgatgagc ttctaaaaaa cagcgatatt gttacgctcc atgtgccgct
      661 ttgtgcggat acccgccatc tgatcggtca gaagcaaatt ggagagatga agcaaggcgc
      721 atttttaatc aacaccgcgc gcggagcgct tgtcgatacc ggggcgctgg tggaggcgtt
      781 ggaaagcgga aagctgggtg gtgcggcact ggatgtgttg gagggcgagg atcagtttgt
      841 ttataccgat tgctcgcaga aagtgcttga ccatcccttt ttgtcgcagc ttttaaggat
      901 gccgaatgtg atcatcacac cccatacggc gtactacact gagcgtgtgc tgcaggatac
      961 tacagaaaaa acaatcagga attgtctcaa ttttgaaagg agtttacagc atgaatagaa
     1021 taaaagtcgc aatcatcttc ggcggttgct cggaggaaca tgatgtgtcg gtaaaatccg
     1081 caatagaaat tgctgcgaac attgatacgg aaaaattcga tccgcactac atcggaatta
     1141 caaaaaacgg tgtatggaag ctatgcaaga agccatgtac ggaatgggaa gccgacagtc
     1201 tccccgccat actctccccg gataggaaaa cgcatgggct gcttgtcatg aaagaaagcg
     1261 aatacgaaac acggcgtatt gatgtggctt tcccggtttt gcatggcaaa tgcggggagg
     1321 atggtgcgat acaggggctg tttgtattgt ctggtatccc ctatgtgggc tgtgatattc
     1381 aaagctccgc agcttgcatg gacaaatcac tggcctacat tcttacaaaa aatgcgggca
     1441 tcgccgttcc cgaatttcaa attattgata aaggtgacaa gccggaggcg ggtgcgctta
     1501 cctaccctgt ctttgtgaag ccggcacggt caggttcgtc ctttggcgta accaaagtaa
     1561 acggtacgga agaacttaac gctgcgatag aagcggcagg acaatatgat ggaaaaatct
     1621 taattgagca agcgatttcg ggctgtgagg tcgggtgtgc ggtcatgggg aacgaggatg
     1681 atttgattgt cggcgaagtg gatcaaatcc ggctgagcca cggtatcttc cgcatccatc
     1741 aggaaaacga gccggaaaa
//
LOCUS       DACECY010000240         1317 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20306_2017
            SAMN12640157-rid9879833.guided.188, whole genome shotgun sequence.
ACCESSION   DACECY010000240 DACECY010000000
VERSION     DACECY010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640157
            Sequence Read Archive: SRR10023221
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741746.1
            Genome Coverage       :: 297x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:27:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,104
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640157-rid9879833.guided.188"
                     /isolate="E20306_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..141
                     /locus_tag="IWO38_003102"
                     /pseudo
     CDS             1..141
                     /locus_tag="IWO38_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            162..245
                     /locus_tag="IWO38_003103"
     CDS             162..245
                     /locus_tag="IWO38_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ0315093.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            370..1107
                     /gene="erm(B)"
                     /locus_tag="IWO38_003104"
     CDS             370..1107
                     /gene="erm(B)"
                     /locus_tag="IWO38_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ0315094.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          494 a          211 c          225 g          387 t
ORIGIN      
        1 aaacagcaaa gaatggcgga aacgtaaaag aagttatgga aataagactt agaagcaaac
       61 ttaagagtgt gttgatagtg cattatctta aaattttgta taataggaat tgaagttaaa
      121 ttagatgcta aaaatttgta attaagaagg agggattcgt catgttggta ttccaaatgc
      181 gtaatgtaga taaaacatct actgttttga aacagactaa aaacagtgat tacgcagata
      241 aataaatacg ttagattaat tcctaccagt gactaatctt atgacttttt aaacagataa
      301 ctaaaattac aaacaaatcg tttaacttct gtatttattt acagatgtaa tcacttcagg
      361 agtgattaca tgaacaaaaa tataaaatat tctcaaaact ttttaacgag tgaaaaagta
      421 ctcaaccaaa taataaaaca attgaattta aaagaaaccg ataccgttta cgaaattgga
      481 acaggtaaag ggcatttaac gacgaaactg gctaaaataa gtaaacaggt aacgtctatt
      541 gaattagaca gtcatctatt caacttatcg tcagaaaaat taaaactgaa tactcgtgtc
      601 actttaattc accaagatat tctacagttt caattcccta acaaacagag gtataaaatt
      661 gttgggaata ttccttacca tttaagcaca caaattatta aaaaagtggt ttttgaaagc
      721 cgtgcgtctg acatctatct gattgttgaa gaaggattct acaagcgtac cttggatatt
      781 caccgaacac tagggttgct cttgcacact caagtctcga ttcagcaatt gcttaagctg
      841 ccagcggaat gctttcatcc taaaccaaaa gtaaacagtg tcttaataaa acttacccgc
      901 cataccacag atgttccaga taaatattgg aagctatata cgtactttgt ttcaaaatgg
      961 gtcaatcgag aatatcgtca actgtttact aaaaatcagt ttcatcaagc aatgaaacac
     1021 gccaaagtaa acaatttaag taccattact tatgagcaag tattgtctat ttttaatagt
     1081 tatctattat ttaacgggag gaaataattc tatgagtcgc ttttgtaaat ttggaaagtt
     1141 acacgttact aaagggaatg gagataaatt attagatata ctactgacag cttccaagaa
     1201 gctaaagagg tccctagcgc ctacggggaa tttgtatcga taaggggtac aaattcccac
     1261 taagcgctcg ggaccccttg taggaaaatg tcctaagtgt ggcaacaata ttgtatt
//
